LOCUS       DACEOE010000001        60512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEOE010000001 DACEOE010000000
VERSION     DACEOE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.001"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..140
                     /gene="mraW"
                     /locus_tag="IW774_000001"
     CDS             <1..140
                     /gene="mraW"
                     /locus_tag="IW774_000001"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HAQ1152383.1"
                     /translation="PDEFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKE
                     E"
     gene            145..546
                     /gene="ftsL"
                     /locus_tag="IW774_000002"
     CDS             145..546
                     /gene="ftsL"
                     /locus_tag="IW774_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1152384.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            547..2739
                     /locus_tag="IW774_000003"
     CDS             547..2739
                     /locus_tag="IW774_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1152385.1"
                     /translation="MSLKNKIRRFMEKKNLNPINNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            2765..3727
                     /locus_tag="IW774_000004"
     CDS             2765..3727
                     /locus_tag="IW774_000004"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1152386.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            3741..5102
                     /locus_tag="IW774_000005"
     CDS             3741..5102
                     /locus_tag="IW774_000005"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1152387.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            5128..6216
                     /gene="murG"
                     /locus_tag="IW774_000006"
     CDS             5128..6216
                     /gene="murG"
                     /locus_tag="IW774_000006"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1152388.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            6231..7451
                     /locus_tag="IW774_000007"
     CDS             6231..7451
                     /locus_tag="IW774_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1152389.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            7607..8932
                     /gene="ftsA"
                     /locus_tag="IW774_000008"
     CDS             7607..8932
                     /gene="ftsA"
                     /locus_tag="IW774_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1152390.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            8955..10196
                     /gene="ftsZ"
                     /locus_tag="IW774_000009"
     CDS             8955..10196
                     /gene="ftsZ"
                     /locus_tag="IW774_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1152391.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEIGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            10213..10890
                     /locus_tag="IW774_000010"
     CDS             10213..10890
                     /locus_tag="IW774_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1152392.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            10904..11503
                     /locus_tag="IW774_000011"
     CDS             10904..11503
                     /locus_tag="IW774_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1152393.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            11518..11805
                     /locus_tag="IW774_000012"
     CDS             11518..11805
                     /locus_tag="IW774_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1152394.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            11854..12636
                     /locus_tag="IW774_000013"
     CDS             11854..12636
                     /locus_tag="IW774_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1152395.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            12657..13364
                     /locus_tag="IW774_000014"
     CDS             12657..13364
                     /locus_tag="IW774_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1152396.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            13626..16424
                     /gene="ileS"
                     /locus_tag="IW774_000015"
     CDS             13626..16424
                     /gene="ileS"
                     /locus_tag="IW774_000015"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1152397.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(16473..17999)
                     /locus_tag="IW774_000016"
     CDS             complement(16473..17999)
                     /locus_tag="IW774_000016"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1152398.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(18014..18661)
                     /locus_tag="IW774_000017"
     CDS             complement(18014..18661)
                     /locus_tag="IW774_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1152399.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(18845..19174)
                     /locus_tag="IW774_000018"
     CDS             complement(18845..19174)
                     /locus_tag="IW774_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1152400.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            19351..20079
                     /gene="sfsA"
                     /locus_tag="IW774_000019"
     CDS             19351..20079
                     /gene="sfsA"
                     /locus_tag="IW774_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1152401.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            20095..21108
                     /locus_tag="IW774_000020"
     CDS             20095..21108
                     /locus_tag="IW774_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1152402.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            21108..22385
                     /locus_tag="IW774_000021"
     CDS             21108..22385
                     /locus_tag="IW774_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1152403.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            22448..25150
                     /locus_tag="IW774_000022"
     CDS             22448..25150
                     /locus_tag="IW774_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1152404.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            25302..25619
                     /locus_tag="IW774_000023"
     CDS             25302..25619
                     /locus_tag="IW774_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1152405.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            25649..25969
                     /locus_tag="IW774_000024"
     CDS             25649..25969
                     /locus_tag="IW774_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1152406.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            26077..27537
                     /locus_tag="IW774_000025"
     CDS             26077..27537
                     /locus_tag="IW774_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1152407.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            27605..27826
                     /locus_tag="IW774_000026"
     CDS             27605..27826
                     /locus_tag="IW774_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152408.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            27857..28039
                     /locus_tag="IW774_000027"
     CDS             27857..28039
                     /locus_tag="IW774_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1152409.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            28039..28452
                     /locus_tag="IW774_000028"
     CDS             28039..28452
                     /locus_tag="IW774_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1152410.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            28575..29756
                     /locus_tag="IW774_000029"
     CDS             28575..29756
                     /locus_tag="IW774_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1152411.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            30145..30218
                     /locus_tag="IW774_000030"
     tRNA            30145..30218
                     /locus_tag="IW774_000030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30179..30181,aa:Arg,seq:cct)
     gene            complement(30287..31426)
                     /locus_tag="IW774_000031"
     CDS             complement(30287..31426)
                     /locus_tag="IW774_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1152412.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(31725..32360)
                     /locus_tag="IW774_000032"
     CDS             complement(31725..32360)
                     /locus_tag="IW774_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152413.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(32473..33108)
                     /locus_tag="IW774_000033"
     CDS             complement(32473..33108)
                     /locus_tag="IW774_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1152414.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(33142..33603)
                     /locus_tag="IW774_000034"
     CDS             complement(33142..33603)
                     /locus_tag="IW774_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1152415.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(33733..34164)
                     /locus_tag="IW774_000035"
     CDS             complement(33733..34164)
                     /locus_tag="IW774_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1152416.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(34182..34502)
                     /locus_tag="IW774_000036"
     CDS             complement(34182..34502)
                     /locus_tag="IW774_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1152417.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            34801..35577
                     /locus_tag="IW774_000037"
     CDS             34801..35577
                     /locus_tag="IW774_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1152418.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            35592..35795
                     /locus_tag="IW774_000038"
     CDS             35592..35795
                     /locus_tag="IW774_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1152419.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            35811..36149
                     /locus_tag="IW774_000039"
     CDS             35811..36149
                     /locus_tag="IW774_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1152420.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            36136..36315
                     /locus_tag="IW774_000040"
     CDS             36136..36315
                     /locus_tag="IW774_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152421.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(36358..36828)
                     /locus_tag="IW774_000041"
     CDS             complement(36358..36828)
                     /locus_tag="IW774_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152422.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            36915..37613
                     /locus_tag="IW774_000042"
     CDS             36915..37613
                     /locus_tag="IW774_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1152423.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            37791..38132
                     /locus_tag="IW774_000043"
     CDS             37791..38132
                     /locus_tag="IW774_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152424.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            38125..38796
                     /locus_tag="IW774_000044"
     CDS             38125..38796
                     /locus_tag="IW774_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1152425.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            38802..39488
                     /locus_tag="IW774_000045"
     CDS             38802..39488
                     /locus_tag="IW774_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152426.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            39491..40240
                     /locus_tag="IW774_000046"
     CDS             39491..40240
                     /locus_tag="IW774_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1152427.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            40252..40521
                     /locus_tag="IW774_000047"
     CDS             40252..40521
                     /locus_tag="IW774_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152428.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            40684..40983
                     /locus_tag="IW774_000048"
     CDS             40684..40983
                     /locus_tag="IW774_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152429.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            40983..41285
                     /locus_tag="IW774_000049"
     CDS             40983..41285
                     /locus_tag="IW774_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152430.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            41325..41690
                     /locus_tag="IW774_000050"
     CDS             41325..41690
                     /locus_tag="IW774_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152431.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            42317..42505
                     /locus_tag="IW774_000051"
     CDS             42317..42505
                     /locus_tag="IW774_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152432.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            42513..42707
                     /locus_tag="IW774_000052"
     CDS             42513..42707
                     /locus_tag="IW774_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152433.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(42740..42952)
                     /locus_tag="IW774_000053"
     CDS             complement(42740..42952)
                     /locus_tag="IW774_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152434.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            43077..43280
                     /locus_tag="IW774_000054"
     CDS             43077..43280
                     /locus_tag="IW774_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152435.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            43277..43549
                     /locus_tag="IW774_000055"
     CDS             43277..43549
                     /locus_tag="IW774_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAQ1152436.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            43550..43735
                     /locus_tag="IW774_000056"
     CDS             43550..43735
                     /locus_tag="IW774_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152437.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            43722..44069
                     /locus_tag="IW774_000057"
     CDS             43722..44069
                     /locus_tag="IW774_000057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152438.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            44029..44505
                     /locus_tag="IW774_000058"
     CDS             44029..44505
                     /locus_tag="IW774_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAQ1152439.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(44651..45379)
                     /locus_tag="IW774_000059"
     CDS             complement(44651..45379)
                     /locus_tag="IW774_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAQ1152440.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            45427..45705
                     /locus_tag="IW774_000060"
     CDS             45427..45705
                     /locus_tag="IW774_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152441.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            45707..46093
                     /locus_tag="IW774_000061"
     CDS             45707..46093
                     /locus_tag="IW774_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152442.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            46090..46470
                     /locus_tag="IW774_000062"
     CDS             46090..46470
                     /locus_tag="IW774_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1152443.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            46582..47034
                     /locus_tag="IW774_000063"
     CDS             46582..47034
                     /locus_tag="IW774_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAQ1152444.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            47031..48758
                     /locus_tag="IW774_000064"
     CDS             47031..48758
                     /locus_tag="IW774_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1152445.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            48822..50009
                     /locus_tag="IW774_000065"
     CDS             48822..50009
                     /locus_tag="IW774_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1152446.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            49975..50550
                     /locus_tag="IW774_000066"
     CDS             49975..50550
                     /locus_tag="IW774_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAQ1152447.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            50563..51927
                     /locus_tag="IW774_000067"
     CDS             50563..51927
                     /locus_tag="IW774_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1152448.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            51929..52210
                     /locus_tag="IW774_000068"
     CDS             51929..52210
                     /locus_tag="IW774_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1152449.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            52188..52514
                     /locus_tag="IW774_000069"
     CDS             52188..52514
                     /locus_tag="IW774_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1152450.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            52504..52842
                     /locus_tag="IW774_000070"
     CDS             52504..52842
                     /locus_tag="IW774_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152451.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            52832..53197
                     /locus_tag="IW774_000071"
     CDS             52832..53197
                     /locus_tag="IW774_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1152452.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            53204..53830
                     /locus_tag="IW774_000072"
     CDS             53204..53830
                     /locus_tag="IW774_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1152453.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            53830..54294
                     /locus_tag="IW774_000073"
     CDS             53830..54294
                     /locus_tag="IW774_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152454.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            54489..56798
                     /locus_tag="IW774_000074"
     CDS             54489..56798
                     /locus_tag="IW774_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1152455.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            56810..57514
                     /locus_tag="IW774_000075"
     CDS             56810..57514
                     /locus_tag="IW774_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1152456.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            57511..60270
                     /locus_tag="IW774_000076"
     CDS             57511..60270
                     /locus_tag="IW774_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1152457.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            60283..>60512
                     /locus_tag="IW774_000077"
     CDS             60283..>60512
                     /locus_tag="IW774_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1152458.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKI"
BASE COUNT        20720 a         9917 c        12506 g        17369 t
ORIGIN      
        1 tacctgacga attccaacca gaactgaaag tcattactcg taaaccaatc cttccttcag
       61 aggaagagct aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa
      121 taagaatgaa agaggagtag aatgatggca gaattaaaga aaatgcaaga ttacgaatat
      181 gatttacctg aattggtgga tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa
      241 gaaatcattc ttccacaatc accggcaaaa cgattaaagc agatttctgc tttggaaaaa
      301 attgctgtgg caagcgtggc gattgctatt gttgctttat gtgttttaac agttatgctg
      361 cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat
      421 aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg tactgaacgg
      481 attaaacaaa tcgcagaaga aaaaggattg tctattaatg acgacaattt aaggaaagtg
      541 aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg
      601 ataaacaatc gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg attgttcttt
      661 ttatttgccg tccgtttctc gtatatcgtt attggaggtc atgttgctgg tacctcatta
      721 gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag taaaagcaaa aagaggaacg
      781 atttatgatc gtaacggtgt ggcgctcgca gaagatgcct cctcttattc aattaaagcg
      841 atactctcaa aaacatatac ctctggggat aaaaaactgt atgttgaaga aaagaatttt
      901 gataaaatag cagaaatcct gcataagaat ctttctatag acaaaaaaga tgcactgaat
      961 attttagagg acggcgcaaa aaaagagctg tatcaagttg aatttggtag ctatgggaaa
     1021 aatatctccc aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct
     1081 ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt ttcttctcat
     1141 tttattggat acgcagttcc agacaaagat gagaatggct tagttggtaa actaggtttg
     1201 gagtctgctt acaatgatat cttgagcggt aaagatggta agattattta tcaaaaagat
     1261 aattttcaaa atcctctgcc aggtacagtg gcagaagaag aaaaagctgt cgacggacaa
     1321 gatatctaca cgactttaga cagcagacta caaagttatt tagaaacact gatggatcaa
     1381 gtaaatgaag aatatcagcc agaagaattg acagcagtcc tgatgaaagc aaaaaccgga
     1441 gaaatcttgg caatgggaca acgacctact ttcaatcctg aaacaatgga aggattgact
     1501 ggaaaagatg ctatctggcg taatttcttg gtacaagata gttatgaacc aggatcaacc
     1561 atgaaagttt tcactactgc agctgctatc gaagaaggag aattcaacga gaatgaaacc
     1621 ttccaatctg gaaaaatcca agtagaagat gctacgatca atgaccatga ctttggtgaa
     1681 aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata
     1741 ctggaacagc gattaggcgg acgatggtat aactatttac aaaaactggg atttggacaa
     1801 agtactcatt ctggattaga tgatgaagta aacggtgctt tacctacatt aaatattgtc
     1861 gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa
     1921 gctttcactt ctattgcgaa taacggtaca atgattcaac ctcgatatat cagcaaagtg
     1981 gtggatcccc aaactggaga agagcgaaca actcaaacag aagttctagg gcaacctttt
     2041 tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag atgttgtcga aagcgaaaac
     2101 tacggtagtg cttatggtgt atatagtgta cctggataca atgtatcggc aaaaaccgga
     2161 acagcccaaa tcgcttctga tacaggggga tatcaaacag gagatacagc ctacctgtat
     2221 tcgattgtgg agatggttcc ttctgaagat ccagactatg tcttgtatct aacaatgaaa
     2281 caccctaaga catatgatcg aatggcattg gctaaaatcg caaatccatt gatgaaacgg
     2341 gcaatggatt ttaaagaaac tgaagaagat tcagatacag aaacaaaaac agaaaaagta
     2401 tccgtagccg attatcgtaa tcttgaagcg gacgttgcag cagctgatgc tcaaaaaagt
     2461 ggactgcaac ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc tactgccaac
     2521 ggtgatcagt tgatctctgg agaaaaactg atcctatata ctggtggtga taagctgatg
     2581 cctgacgtaa caggatggtc aaaagcagat atcatgaagc tggggaaaat cctcggtatt
     2641 gaagttagtt ttgatggaga tggttattgt gtcaaacaag aacttgctcc atatgaaaaa
     2701 atcactgaag acaaacttag ttttacttta gaagaataag aagagaaata ggagagaaaa
     2761 gagcatggaa tggacacaag cattgattcc cattgtcagc agttgtgcaa tgacaattgc
     2821 cgcaatgccg ctatttattg gttattttca aatgaaaaag caaggtcagg cgattcgtga
     2881 ggagggacca aagtggcaca atagcaaagc cggaactcct acaatgggcg gcttagtgtt
     2941 tctgatcggc tctattttga caggaatttg ggttggagct tggcaaaaac agctaactcc
     3001 tactcttttc atcttgcttt ttgtattagc gttatacgga gtaatcggct ttcttgatga
     3061 ttttataaaa atattcaaga aaagaaacat gggactgaac tcgaagcaga aattgattgg
     3121 acagattcta gggggaatca tcttttatct tgtttaccgt tctgaaggtt atccaggtgt
     3181 attgaatttc ttcggactag atctaccttt aggaatcatt tatggtattt tcgctctctt
     3241 ctggcttgtt ggattttcaa atgcagtaaa tctgacagat ggcatcgatg gattagttgc
     3301 tggcttagga agtatctctt ttgcagctta cggtctaatt gcttggcatc agcatcagta
     3361 tgatgtatta atcatttgct taagtgtttt aggaggttta ctgggtttct tcgtctacaa
     3421 cagaaaacct gccaagattt ttatgggaga tgtcggttca ttagcgttgg gcggactttt
     3481 agcggcaatt tctataatgc taaaccaaga atggacattg cttttgattg gtttgatcta
     3541 tgttatggaa acagcaagtg tgatgcttca agttacttcc tttaagctta caggaaaacg
     3601 aatttttaaa atgtctccaa ttcatcatca ctttgaaatg tgcggatggt cggagtggaa
     3661 gattgataca gttttttggt tgatcagcat tgtgacttcg ttgatcacat tatggatcgt
     3721 ttggtaggag atgacgggaa atgaaaaaaa tatcaacata tgaaaataag aaagtgcttg
     3781 tcttaggact cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa ttgggcgcac
     3841 ttgtcaccgt taatgatgga aaaccttttg acgaaaatcc ggaagcacaa gagctgcttt
     3901 ctttagggat aaaagtaata acaggtagtc atccaatcga attgttggat gaagaatttt
     3961 cattgatggt caaaaatcca gggattccct acagtcatcc tttagtagca aaagcccaag
     4021 aaatgggtat tccggtaatt acggaagttg agctagctta cgaagtagcg gaatgcccaa
     4081 ttattgggat tacaggaacg aacggaaaaa caacgacaac aacgatgaca ggactactac
     4141 tgaatgcagg agcagatcaa ggaattgcac gtttagcggg taatattggg tatccagcaa
     4201 gtggtgtagc gcaagaagca aaatcagaag ataaaattgt gatggaattg tctagcttcc
     4261 aactcatggg aatcacagat ttccgccctc atatagcggt gattaccaat atctatgaag
     4321 cacatattga ttatcatggt acaagaaaag aatatgtcaa agctaaatgg aaccttcaaa
     4381 aaaacatgac agaaaaagat tatctgatcc ttaactggaa tcagtcagaa ctacaagaat
     4441 tagcgcaaag aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg ttggaagacg
     4501 gtgtgtatgc tgacgattac tcaatatact ataaaaaaga aaaaataatg gaaatatctg
     4561 aacttggcgt accaggtaag cataatgtag aaaatgcact agctgctatt tctgtagcaa
     4621 aattgtatgg tatatcaaat gaagctatac gagaaacgtt gcattttttc catggcgtac
     4681 cacatcgtac gcaatatgta ggagaaatcc aaggcagaaa attttataat gattcaaaag
     4741 caaccaatat tttagcgact aaaatggctc taagtggatt tgaaacgtct aaggtcgtgt
     4801 tattggctgg cggattagac agaggaaata catttgatga attaattcct tcattaaaag
     4861 gaatcaaagc aatggtcgta tttggccaaa caaaagaaaa gttgatggat gctggaaaaa
     4921 aagcaggcat cgagacaata gtaacagccg attctgtaga acaagcagtt cctttagcat
     4981 tagaaaacag tacagatggt gatgtagttt tattatcacc agcgaatgcc agctgggatc
     5041 aatacccaaa ttttgaaact agaggaaaca gattcatgga agctgtgaac cgattaaaat
     5101 agaaaaagta gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg
     5161 ggacatattt atccagctct tgcctttgtc aattatgtaa agactcaaga accgaatgct
     5221 gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga
     5281 atgcctttcc ataccttgga gattcaagga tttaaacgaa aaatttccat gcataacatc
     5341 aagacggtcc gattgtttct taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt
     5401 caaccagata tcgttatcgg tactggagga tatgtttccg gagcggtagt gtacgctgca
     5461 gccaaaatgg gtattccaac agttatccat gaacaaaaca gtgtaccagg tgttaccaat
     5521 aaatttttaa gccgatatgt ggatcggatc gctctttcat ttaaagatgc ggcttccttt
     5581 tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag cacaagaagt agcagatact
     5641 aaaaaatcag atattttgaa aacatttgat ttggatccag aaaagaaaac agtcttgatt
     5701 tttggcggta gtcaaggagc acttaaaatc aatcaagcag tatcagtttt tttaagtgaa
     5761 tatattcctt cttcttacca agttttgtat gcttcaggag atagatatta tgaagagatc
     5821 ctaaataaaa taaatcctga aatacaaaat attagtgtcc agccttatat caaaaatatg
     5881 gcagaggtca tggcttccag tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca
     5941 gaattcacag ctctggggtt accggcagtg ttggttccca gcccatatgt tacgaatgat
     6001 catcagacta aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat gattgcagat
     6061 aatgaactaa caggtgaatc actaagtcag acagtcaacg aaatcatggg tgatgaagaa
     6121 ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt
     6181 ctttatgact tagtaaaaca aataatcaaa aaataaaggc ggtggtctta gtgagtaaga
     6241 aaaaagaacc aaaaaaattg tctcatccgt cttctgatga aaacaatttg actccttggc
     6301 aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag
     6361 aagacataac attagataca cccctttcag attctgaaga agaaaaaacg gttcaagagg
     6421 aacagcaaga agaaagccag tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa
     6481 agaaaaacgg tggaccatat aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc
     6541 aacggcacaa agtactattt cgtcggttag ctttgatcat cactgtattg ggtataccgc
     6601 tgattttttt gatctattat gtttctcctt atagcaaatt acaagcagtt gcagtatcag
     6661 gcaataagac agtcaacagc caagaaatca tctctgatac aaaattgtca ttaggagaaa
     6721 atgtttgggg acagtatttt catcgatcta cttatatcga tcgccttaaa aaagcacagc
     6781 caagaatcga aacagcaaat atacatttca aagggatgaa tgaattcgat ttggatgtaa
     6841 cagaatataa agagattgct ttgatcgcaa aaaacaatca atactaccca gtgattgaaa
     6901 atggaacagt attagatgaa aaagtagcaa atcctacaaa aaatctccct atcttggaag
     6961 agttcaaaga ctctgcaaaa atcaaagaat taacaaagca atacaatcag ttatcatcag
     7021 aattacaaaa agcaatttct gaaatcaagt acacgccaag agcaagcaat aaaaatctga
     7081 tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc
     7141 aaatgaaata ttattcgcaa gtagccaaag atatggatga aaaaggtgtg atcgacatgg
     7201 aagttggtat cttctcgcat ccttacggta gcactcaaga aacggaagaa tcgggccaat
     7261 cggaagaaac aactgaaagt gaagaaaatg cggatcaaac aaacgaatca agtgaagagg
     7321 cagaaacgca aaaagcacaa acacctggat cgtctagcac aaacggatca tctgattcaa
     7381 atgattcaag tgaaacaagt caaatagaag ataatccaag tacaactccg tcaagtttaa
     7441 atgaaaattg ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt
     7501 atggtagaat tggaagcagt gaatatatct attaataagt atattttaaa acggacgcta
     7561 gttgttcgct tgtatatgga atagtaagta ggagggaatc ccattcatgg caaaaacagg
     7621 aatgtatgta ggccttgata ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat
     7681 cgacagccaa atgaatatta ttggagtagg aaacgcaaaa tcagaaggga ttaaccgagg
     7741 cattatcgtt gatatcgata aaacggtcca agcgatacaa cgagcagtac gacaagcaga
     7801 agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga
     7861 agtagaaaat tgccagggaa tgattgcagt taacggagat tcaaaggaaa tcaccgatga
     7921 agatgtcaga aatgtggctt cagctgccct tgttcgttca atacctcctg aacgtcaaat
     7981 cgtgtcaata ttgccacaag attttactgt cgatgggttc gagggaatca aagatcctcg
     8041 tggaatgatc ggcgtaagat tagaaatgta cggtctgttg tttacaggac ctaaaacgat
     8101 catccataac atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat
     8161 tactccttta gcgctgactg aatcaattct ttctgacgga gaaaaagatt ttggtacgat
     8221 cgtcattgat atgggtggcg gccaaacaac aacggctgtt atgcatgata agcaattgaa
     8281 gtttaccaat cttgaccaag aaggtggcga atttgtcact aaagatatct ctattgtatt
     8341 aaatacgtca tttaacaatg cagaagcgtt gaaaataaac tacggggatg catacccaga
     8401 acggacatca ccagatgaag aatttccagt agatgtgatt ggtcagtcag aaccagtgaa
     8461 agtcgatgaa cgctacttgt cagaaattat ttcagctcga atggaacaga ttttcaacaa
     8521 agcaaaagat gcattggacc aaatcgaagc attagagttg ccaggtggcg tcgtattgac
     8581 aggcggagct gctagtcttc ctggtgttgt ggacttggca caagaaatct tcggcgtaaa
     8641 tgtaaaattg tatgttccaa atcacatggg gctgagaaac ccggtattta caaatgtaat
     8701 aagtatcgtg gattattcag caaacttgag tgaagtgtat caattggcaa aaattgctgt
     8761 tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa caaacacata
     8821 tgagagctat gaagcacctg aagaaacaat ctacgatgag ccggaaccaa aagagtccgg
     8881 agaaaatgta aaaaataaaa tcaagggttt ctttacaaat atttttgatt aattcatcct
     8941 gggaggaata agatatggaa ttctcaattg ataataatat caatgacggc gcagtaatca
     9001 aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg
     9061 ttaaaggtgt tgaattcatt acagccaaca cagacgtgca agcattaaaa aattcaaaag
     9121 ctgaaacagt tatccaatta ggccccaaat atactcgtgg tttaggtgct ggttctcagc
     9181 cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg
     9241 gtgcagatat gattttcatc actgcaggaa tgggtggagg aaccggtaca ggtgctgcac
     9301 caatcgttgc aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc
     9361 cattcacttt tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc gctcgtttga
     9421 aagaaaatgt ggatacatta ttaattatct caaataaccg tttattagaa gttgtagata
     9481 aaaagacacc gatgcttgaa gctttccgtg aagcagataa tgttttacga caaggtgtac
     9541 aaggtatctc tgatttgatc acagcgccag gttacgttaa ccttgacttt gctgacgtga
     9601 aaacagtgat ggaaaaccaa ggtacagcat tgatggggat cggtgtggct agtggagaag
     9661 atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa acatctattg
     9721 atggagctga acaagtactt cttaacatca caggcggttt ggatatgaca ttgttcgaag
     9781 cacaagatgc ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat atcattctag
     9841 gtacatctat caatgaagaa ataggcgatg aaatccgtgt gacggtcatc gcaacaggta
     9901 tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca caaatgcaat
     9961 cacctgctca aaagactgta ttagatatgg atcaagctaa accaacttct tcagaagaag
    10021 aaaacagttt tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct agagttgatg
    10081 attccaattt tgacaatatc gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa
    10141 aatctcatgg cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga
    10201 gaggaagagc acatgattgc tgacaacttg cagaaaatca agcaagaaat tcaggattcg
    10261 tgtgctcttg ttcatcgtga tccgaaagaa gttacattag tagctgtaac aaaatctgtt
    10321 gaatccgctc aagctgaaga attggttaga gctggcgtta tgaattgtgc agaaaaccga
    10381 gtagataaat tattagaaaa gaaacaagat ttatttgatt ttcctgaaat aaagtggcat
    10441 ttaattggta atttacagcg cagaaaggta aaattgattg taaatgaaat tgattacttt
    10501 catgcgttgg acagtttaag attagcagaa gaaatccaga agcggttaga gaaagaatta
    10561 tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat ttcaccagat
    10621 gagcttttac cgtttgtgga atctttagct gcctgcacaa aaatcaaggt tgttgggttg
    10681 atgactatgg cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt tggtacgctg
    10741 aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta
    10801 agtatgggga tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac atttatccgt
    10861 gtaggaacag cgctattcaa agacgaataa aggaggaaca aaaatgccgc ttttaaacag
    10921 aggagcaatt gctaattttt tcggattagc tgatgaggaa gagtacgaat accaagatta
    10981 tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac agaatcaatc
    11041 aactcattct gtatctgctg cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc
    11101 tgtaacgcat gaaagtattc agccggctga aaagaaaaaa gtaattgaac tacatcaatc
    11161 acctacaaaa actgtagcga aagaaagaac agcttcaaga acctctacac aacctaaagc
    11221 aaatcttgct ggaaaaatca ctgtaatgga accaagagct tattctgaag ctatggcgat
    11281 tgctaaacga atcattgctg gagaagcaac cttagtgaat tttcatttag tagaagaaaa
    11341 acaagcgaga agaatcgttg attttctaac aggaacagtt tatgcactag atggagatat
    11401 caaacgtgtt ggagatgaga tttttctttg tacaccgact gggatggaga ttgacagttc
    11461 aaccgctcaa tcttttgcag atgcgaattt gtttgatctg taagaggagg aactacgatt
    11521 tatactattg cactattact tcatttactt caacgggcag cctatttcta tagcattatc
    11581 ttgatggtat atgcattgct ctcttggttt ccaggcgggt atcaatcagg gctaggacga
    11641 ttattagcta agatctgcga accgtatatc agtttgttcg atcgtctgcc tttgaggatt
    11701 ggacccttgg atttcacaat tttaggggca atcctgatct tgaacttagc cgtacaaggt
    11761 ttagcacaga tcgttatatg gataactgtc catttagttt attgattgaa gcaacaaaat
    11821 ctttattctc agaatagata ggaggggaaa attttggatg caaatgtata ccagcacttt
    11881 cgggcagatg aacgtacatt tatcgattcg gtaggcgatt ggattgaaca ggttcaagga
    11941 caatatgcac cctatctgac cgaatttctt gacccaagac aatcctatat tttagaaaca
    12001 ctgattcgac aaagcagtga tttgcgtttc atgttttatg gcggatatga acaggcagaa
    12061 agaaaaagat gtttgatttt ccccgattat tacgaacctg tagaggaaga ttttgaaaca
    12121 gcattgtatg aaatccatta tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt
    12181 ggaactttag tgggaacggg gatcaaacgc tcttactttg gcgatatcat ctcggacggt
    12241 gatcgctggc aagtatttat tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca
    12301 aagattggaa aggttgctgt acgcttggaa ccaatcaaat acacagaaat gattatgccg
    12361 aaagatagct ggacacaaga aagaggaaca gttagttcca tgcgactaga cagtgtgatc
    12421 tctgagattt ataatatttc cagacagcgg tccaagcagc tgatcgaagc tggtaaagta
    12481 aaagtgaact ggacagaaaa tacaagacct gattttgtac ttgaactatt agatattatt
    12541 tcagtcagag gttatggcag agtccagatc caagaacttg aggggaagac gaaaaaagaa
    12601 aagtacaggg tactgttcgg tgtattacgt aaataaaata aaaaggtggt ttactcatgg
    12661 ctttaactcc attagatatc caaaataaga cattcccgac aaaaatgcgg ggatataatc
    12721 aagacgaagt agatgatttc ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa
    12781 gaaatcgaga attagaaaaa gcagtcaaac attctgaaga aaaacttgag tacttcaatg
    12841 aattaaaaga tgctttgaac caatcaatca tcgtagctca agatacggct gataaagtaa
    12901 aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac aaagcagatg
    12961 agttagttgc gaacgctgaa aaacgtgcac atcaattaac aactgatgca gaagaaaaag
    13021 caagaaaaat cttgacagat gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag
    13081 atcttaagaa aaaaacacga gtattccatc aacgtatcag cttgatgctt gagtctcaat
    13141 tagaacaagt gaaaagtccg gaatgggatg aaattttaca accattctcc agctacgtga
    13201 ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat gaaaatgata
    13261 ctgatgtaaa ctcagacaca ggagtcatag aagcacctat gacggcattt gatacacagg
    13321 ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga aaagcacttt
    13381 gttattctta aagcgagccg atgagagtgg aagttcggta agacactgca tggtgaaaga
    13441 tcctctactt gagacctgct tttccgaaat tagagtaaga agcagcggct gacctcgtta
    13501 ttggtcacag agagtttgca ctagatagat gcaactaaat ttgggtggta ccacggcatg
    13561 ttcgtccctt gcggctgaac atgctttttt gtgtccggaa aacaaataaa ggagtgtatg
    13621 gttgaatgaa aatgaaagaa accttgcaat taggaaaaac aaaattccct atgcgtggta
    13681 atttgccaaa ccgagaaggc gagtggcaaa aagaatggga agaaaatcga atctaccaaa
    13741 aaagacaaga gttgaacgaa ggaaaaccta gctttgttct tcatgatgga cctccatatg
    13801 ccaatggtaa tatccattta ggacattcat tgaataaaat cagtaaagac attatcattc
    13861 gttcaaaatc aatgtctggc tatcgtgcac catatgttcc aggatgggat acacatggat
    13921 tgccgattga acaagtattg gctaataaag gcgtgaaacg taaagaaatg tccatggcag
    13981 aatacttgga aaaatgtcgt gaatatgcac tttcccaagt agataagcaa cgagcagatt
    14041 tcaaacgcct aggagttgca ggagattggg aacatcctta tgttacatta gatccttctt
    14101 atgaagctgc acaagtacgc gtatttggta aaatggctga aaaaggctat atttacaaag
    14161 gattgaaacc aatttactgg tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg
    14221 aatataaaga tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa gacggcaaag
    14281 atattcttga tgaagataca gcttttgtca tttggacgac aacaccatgg acattgccag
    14341 caaaccaagg tattgctgta aatccaaaat ttacgtatgt attggtggaa gccgatggcc
    14401 gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca atcagaaatc ggttgggaaa
    14461 atgtaacagt tctaaaagaa tttgctggac aagaaatgga atatatgact gcaagtcatc
    14521 cattctatga acgtgaatca cttgtgattt taggcgatca tgttacttta gacgctggta
    14581 ctggattagt ccatactgct ccaggacatg gggaagatga ctatatcgct ggtagcaaat
    14641 ataaactatc tgtagtgtca cctgtagact caaaaggatt gtttacagaa gaagcacctg
    14701 gatttgaagg tatcttttat gacaaagcaa atcctatgat cactgatcta ttaaaagaaa
    14761 aaggagcttt gctgaagcta gatttcttta ctcatagtta tcctcacgat tggcggacga
    14821 aaaagcctgt gatctatcgg gcaactccac aatggtttgc atctatcgat aaattccgtc
    14881 agaatatttt ggatgaagta gaaaaagtag agtgggttat cccatggggt aaaacacgtt
    14941 tgtataatat gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag
    15001 taccacttcc gattttctat gctgaaaatg gagaagcaat catcacacca gaaacaatcg
    15061 aacatgtggc aaaattattt gctgaacatg gatcaattat ctggtttgaa aaagaagcga
    15121 aagaacttct tcctgaagga tttacccacc caggttctcc taatggcgaa tttacaaaag
    15181 aaaaagatat catggatgtc tggtttgatt ctggttcttc tcatgaagct gttttacgtc
    15241 agcgaccaga attatcattc ccagcagata tgtatttaga aggatcagac caatatcgtg
    15301 gctggttcaa ctcaagtatc acgactagtg tggctatcaa tggtgtagca ccatacaaat
    15361 cagttatttc tcaaggattt gctttagatg gtgaaggccg taagatgagt aaatctttag
    15421 gaaacatcat tgcaccagaa aaagtgatca aacaatttgg tgcagatatc cttcgcttat
    15481 gggtatctag tgtagattat gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag
    15541 tggcagaagt atatcgtaaa atcagaaata cgatgcgttt cttattagcg aatacagaag
    15601 atttcgatcc taaaaaagat actgtttcgt acaatgattt acgttctgta gacaaatata
    15661 tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga agaagggtac gacaaatata
    15721 acttcatgca tatttaccgt acagttatga acttcttgac ggttgatcta tcgtcattct
    15781 acctggattt tgctaaagat gtagtttata ttgaagcaga agatgattat caacgtcgtt
    15841 gcatgcaaac tgttttctat caaacagctg tagctttgac aaaattattg actcctatca
    15901 ttccgcatac agcagaagaa atctggtcat tcctgaaaga agaagaggag tacgttcagt
    15961 taagcgagtt tcctggttat gaagagtttg caaaccaaga agaattgatg gatacctgga
    16021 ctgctttcat ggacttccgt gataatgtct taaaagcatt ggaagaagca agaaacagca
    16081 aattgattgg taaatctttg gaagcaaaac taactgttta tccaaaacaa caagttcgtg
    16141 aaatgttaaa agctttggat gcagatattg cacaattatt gatcgtttca ccagactact
    16201 ttgaagttat ggcagcagat gaacaagtgc cagataacgc tatggtattt gatgatgttg
    16261 ctattacagt ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag
    16321 atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag
    16381 acttctatcc tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg
    16441 tgacatgttc tttgtcacac ccccttttgt tatcaatcgt ttaattttcc acgttcacga
    16501 taccagttat ctggttgcca gatccattcg aatccgtctt tttcaagtag ttcgaaagct
    16561 gctttaggtc ccattgatcc agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa
    16621 gcttttcgaa tgacatccac gatatgccaa gattgagcga cttcttccca atgagagaag
    16681 ttcgttccat caccattcaa cgcatctaat agtaattttt cgtaagcttc tgggctattt
    16741 tcaaccattt ctgcactatt tctaaattcc aatttgattg gttccgtttc aaatccttgc
    16801 cctacttctt tcccattcaa ggataaagaa aatccttcag ttggctgaat atagatcgtt
    16861 agaacattag gtggaagtgt cgtatcgtca catggttcat cgacagatgt tttgaataca
    16921 ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc ctttttcagt caaacgtttg
    16981 cctgtacgta catagaaagg aacacctgac caacggaagt tatcgatagt gaatttgcct
    17041 gcgacgaatg tttcagtctc tgattgagca tccactttgt cttcttcgcg atagcccttg
    17101 aattgtacac catccaattc accttgagca tattgcccgc gaacgaagtt ttctaacact
    17161 tcttcttcgt tataaatgcg gatagctttc aatgctttga tcttttctgt acggatttct
    17221 ttttcggaaa atgctactgg cggttccatt gcaagtaatg ctacaacctg taaaatatga
    17281 ttttgcacca tgtctttaag tgcgccacta tgatcgtagt agcccccgcg atcttctaca
    17341 ccgaggtttt cgccaaaagt aatttggata ttgtcgatat agcgattgtt ccattgagcc
    17401 tcaaaaatat tattggcaaa acggatagct gagatgtttt ggatcatttc ttttcctaaa
    17461 taatgatcaa tcctgaaaat atcttgttct gggaatactt ctctgatttg ctggttcaat
    17521 tcaaaagcag actggtaatc agaaccgaac ggtttttcta tgatcaaacg attgaagcca
    17581 ttttttgtca taatattttg tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt
    17641 gccatagcca aataatagac acggttaccg tttaattgat agtgtccatc taatcggtca
    17701 gacaattctt ttaatgtctg ataatgcgtt gaatcattta catcatggga ttgatagtaa
    17761 aaatggcttg caaaggtagt tgcttcttca tctgaaggat tcaatgcacg gattgtttct
    17821 ttgacaactt cacggtagtg ctcatcgctc cagggacggc gagcggttcc gattacagca
    17881 aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca aagacgggta caatttacgc
    17941 tgggctaggt cacctgtacc gccgaatatc gtaaacaata catttttttc attagacatg
    18001 tggatacact cctttatatt ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg
    18061 ggattttctt tctatcattt tggatagtga tcggtccttc ataagctgcg atgtcaacga
    18121 tctcatattc ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac aattcttttt
    18181 catcaagcac tcgtgcgata cgcacttttg taccgacagg ataggactcc aatgtttgtc
    18241 ggcgttcttc atggacaact tcctcttttt taggaatcga tcctccatga ggacaatgtt
    18301 caggatgatt caaatagttt tctaaatgat ctgcaagatg ttccgaagtc acatgttcca
    18361 atacttcagc atcatcatgg acttcattcc agctatagtc taaatgttca actaaaaaaa
    18421 cttcccataa gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg cttgcagtca
    18481 attgtacacc ttgataaggg gaatgttcta caagctcttc tttcacaagt ttagaaatca
    18541 tttcactgac agatgcagct gagacatcta gactagatac gatttgttta ttgttgactt
    18601 tcgttgtatc tccacctagt tctaaaatga ttttcaaata atcttctcga tttggtgtca
    18661 tattcgatca tcctttcatg gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt
    18721 aattgactat ttttctaaat aaaaaacaaa ggatttttag acgtttttac aacaaaaaac
    18781 gaaagatgat agagaagact cattctttcg tttataaagg atatatatat acattgggag
    18841 ggatctattt ttcagataac aacttatatg aagtgtctgc agattcgtca taaagagttt
    18901 gttcgggata agaaaagata agataaaaac catactgatc acgtacgact aaacgatatt
    18961 catctatttc taccacagat gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag
    19021 atgtaatctg taattgttgt ttttgctggc tatcactaaa gaaccatgta ccttgatact
    19081 ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt caagtaaata aaacttgaga
    19141 gaacggtaat gagtgaacca agaaaataga tcattttttt tatcaccaaa tcactccttt
    19201 atgagaaaat aatcgcatag acatattctt tcttctttat ttcattgtac cgaataaaag
    19261 ctaaaatttc tctaagactt tcgactggtt tttttactgt ttttctacaa attatgatag
    19321 gatacatagt aggaagaaag gtgacataat ttgaagtatc agcaagtgga aatagctttt
    19381 tttattgacc gcccgaatcg gtttatagca cattgtatga aaaaagacgg agaaataatt
    19441 attgcgcatg tcaaaaatac aggtagagga aaagaggtcc tgataccagg cgctgaagtg
    19501 gctattgtat ttgctcctgg acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa
    19561 aagaagggtg actggtttaa tatcgatagt caattgccta atcggctggc aattgatggt
    19621 attttagacg gcacgatcct tttaccagga ttaactggtc catttacggt atacaagcgt
    19681 gaagtaactt atcttcactc caaatttgat atttatttag agacggtggt cggacaaaaa
    19741 gcatttgttg aagtcaaagg tatgacactt gaaaataaag cgattggtgc ttttcctgat
    19801 gcgcccacac tcagaggatt gaaacatata ggagaattaa cgtatgccgc acaagatggt
    19861 tatgccgctt atgtcttgtt tattgctcag ttcgaacatc ttcatctggc aacgatccat
    19921 gaagaaatgc aaccagcact agctgatatg gttcgacatg cgcagcaaag cggagttcaa
    19981 atcctcgcct ataattgtca agtatcggca gatgaggtaa ctgtcaagca ggccattcca
    20041 tttgatgtag atatcccttt tgaagatcct atacaataga aagaaggtaa aataatgatc
    20101 aacttaggta ttatcggaac aaattggatt actcatcaat ttgtccaagc agctttaagc
    20161 acaaaaaaat atgaattgac agcggtatat tctagacatt tggaaaaagc acaggaattt
    20221 ggttctcatt atgaagggga tatcgaattc gcaacagatt tgaagacttt tttcaatatt
    20281 actcatatgg acaccattta tattgcttct ccgaatagtc ttcactttag tcaagctaag
    20341 caagcaattt tggcaggaaa aaatgtgata gttgaaaagc cagctttttc gacgccaaaa
    20401 gaaatggatg aaatcatcca tttggcaaat gaacacaatg tgtatttttt tgaagcagca
    20461 agaaatatcc atgaatcagg ttttaaaaag attgccgatt acttaccatt aaaagacgag
    20521 ataatcggtg caaatttcag ctatatgaaa tattcttcgc gctatgacca agtattagat
    20581 ggagaagaac cgaatatctt ctcgactcat ttctcaggtg gtgcattgat ggatttaggt
    20641 gtttaccttg tatatgcagc tgttgcttgg tttggaatgc caaaagaagt ccattatttc
    20701 ccaagaaaga tttctactgg tgtagatggt cttggatggg ggatcttacg ttatgagaat
    20761 tttgatgtat cgatccagcc aggtaaaatc ggtgattctt atttaccgtc agagatttat
    20821 tttgactcag gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat
    20881 gatcgcgcgc ataaagaaat cattgatttg gcggtaacag ctgcagaaaa tccaatggca
    20941 gatgaagcag aggattttgc caatgtcatg gctcatccga aagatccagc ttggggattg
    21001 ttgtatgaag agtggatcga gctagctaga aatgtgaacc aagtgattta tgatttaaga
    21061 aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag atttttaatg aacgaatttg
    21121 tacaagtatt tcccgaaaat tggcagaaaa aatatgagga taaagggttt atctcacctt
    21181 ctcttattca acgagcagtt tttcagccac taagtaataa acaaagtata gtagcggttt
    21241 ctcctacagg ttcaggtaaa acactggctt atctttggcc tctactacta aacgtggaac
    21301 caggtgaagc aagtgcgttg gtcatttttg cttcttctca agaactagct attcaagtag
    21361 cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa agtccaatct ttggtgggcg
    21421 gagccaacgt caaacgtcag attgagggtt tgaaggaaaa accagaaatc ttgattggta
    21481 caccaggaag aatattggaa ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa
    21541 caatggtatt tgatgaagca gaccagctat ttgacgctgg gaacagccaa atcattgatc
    21601 aaattcttca tcaagctcca acagagtatc agctagcttt tttttcagcg acagcagatc
    21661 gttcccttga aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag
    21721 cagaggacga ttctcgaaaa ggactgagac attacttttt acgtgtaccg atacgaaaga
    21781 aagatgacta cttgagacgt ctcacgcata tagaagattt tcatggtctg attttcttta
    21841 atcaactgac tgaattaggc gtaatggaag aaaaattgct gtatagaggt gtaccggtag
    21901 gaagtctggc ctctgatcag aacaagctac tccgaaaagc agcgctagaa caattcaaaa
    21961 agaaaatggt cagagcactt ttgaccacag atgtggcggc tagaggttta gatattacag
    22021 gtcttcctta tgtagtgaat gcagaagttc cgttaagtga agaagcttat ttgcatcgtt
    22081 cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat tacactcgta caagataata
    22141 atctaagaga tttaaaacgg cttttacgtc catcaaatat aacacttgaa gagatttttt
    22201 tatacggagg taagctgcaa actgagcagc caaccaaaga agatgcagct cctgcagtaa
    22261 ataaaaagta tgcgaaaact ttttctggaa aggagcagaa cccagaatat aacaaagaaa
    22321 aaataagaaa aaaagttaaa aataaatcaa aaaaagagag aaataaagga gcaaggcgca
    22381 aataagcgcc tttttatgct atgattatga aaacgggaac aaaattggtt gccagaggag
    22441 gggttatgtg aaagaacgtc aagaagagat cttgcagcta ttgacagaaa accaacaagg
    22501 attgacagca agtgatgtag ctgagcgctt aactatcgat cgaagcaatg caagccgata
    22561 tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc gctggccgac cagtcgttta
    22621 ctctttgcca acggaaaagt caaaatctga tgaagtgcat gtcgactctt ctacgcaagt
    22681 gacttttgaa acactcgttg gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa
    22741 agctgcgatt ctttatccac cgcgggggtt gcatacgatt atttttggag aaactggtac
    22801 agggaagtcg atgtttgctg aatgtatgta tcattttgca atcgattcag agatgctttc
    22861 tgcagatgcg ccatttgttt ctttcaattg tgcggattat gctcaaaatc cgcaactgct
    22921 ctttggacac atctttggga tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc
    22981 cggtctaata gccaaagcag acggcggtat attgttttta gatgagatcc accgtttacc
    23041 gccggaaggg caggaaatgc tgtttacttt cattgataaa ggtatttacc gtccgcttgg
    23101 agaaagtgca caagtacatg aagcatctgt tcagatcatt ggggcgacga ctgaatcttc
    23161 agaaagtttc ttatctacgt tcaatcgacg gattcctatg gcaatcacct tgccgaatct
    23221 tgcttcacgt tcactggatg agcgttatga aatcatctct ctttttatca aacaagaagc
    23281 gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg attcttgcct tcatgcttta
    23341 tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc
    23401 ttttttacat taccgtactc atcatgagaa aaagctaatc attcgaaaag aagaactttc
    23461 ccttcaagta caaaaaggat tactgaaaat caaggaaata ccggaacgtc tcgaccgatt
    23521 catggacagc aaaagtcagt atttgacatt tgaaccagga tttgctgatg ttgtctggtc
    23581 acaagatcca gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag tcacttctct
    23641 ttccgaaacc ggtgtggaga acattgatct agagaccctc atttcaaaag atgtggatgc
    23701 ttatttccag acatatgtca aagaattgac taaaagttcg attcccaaag atcttttgcc
    23761 agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg
    23821 tatttataat gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta cattggatcg
    23881 tgtgagagaa ggtcatatga tcgtccaccc agatttgaat aacgtacgga aaaattttaa
    23941 gagggaattc caggtagcaa tcgatctttc aacgatcatt gaagaagaag aacaagttga
    24001 gattcctttt gatgaaatcg gctttattag tatgtttctt tctatcaatc taggagaaag
    24061 tgagcctgtt aaagaaaaca aagtagatgt tctcatattg atgcacggca gacagactgc
    24121 ttcaagcatg ttggagatgg cacaagaact attaggaaca aaagtaggga aagcttttaa
    24181 tatgccactg gttatggaag ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa
    24241 ctatcaagag catttgacga atggcctact cttgttaaca gatatgggat cattaaacac
    24301 cttcagtgat ttgatttatg aagagactgg cattcggacg aaagctatca cgatgacaag
    24361 taccatgatt gtgatagaag cgatacggat ggcaagtgtt ggaagaagct tagaagatat
    24421 ttatcaaaat atccagcttt cctttgaatc gatcgtacgt gatcagttca aagcggaaaa
    24481 gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt
    24541 agctgccaaa ttatatcagc gtattatgcc agtgattaat caaaataagg tagaactgat
    24601 ccagatgcag tttttagaac gtgaatcatt caaaaaacac atcgatgcct tattggaaga
    24661 atatgagatc aaagcaattg cgggaacagt tgatgttgat tatcaaaata tcccattttt
    24721 ttcagcctat gacgtttttg atgatgaaaa actaaatatt ttaaagcgta tagctgatga
    24781 tgagatcccg acagctaaaa ttgtcgattc acttgctgga tcattaagcg atgtcttttc
    24841 tattgatgaa ctgatccgtt cattgcaaaa gacagttcag cagatccaga ccgatttgtc
    24901 gattatcgtg gaacctggag tagatactgg aatcgttatc catttagcct tcatgttaga
    24961 cggattgatc aaacaagaaa gccatcggca atttgagaaa ctgtctattt tctcaaaaat
    25021 ttatcgtttg gaaatggata tcgttcgggc aaacttgatg gcactagaac gaaaatatca
    25081 ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa atgtttcttg aaaataaaat
    25141 aaaaaaatga acacactaat catactgttt acacactaaa catctgtgtg cgcactataa
    25201 aaaagagcta aaaagcatga aaacgctatt tcagctcttt tttttattgg catgcttctt
    25261 gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca
    25321 tgttagtttg ttcagcaggg atgagtacga gtttattggt cacaaaaatg caaaaagcag
    25381 cagaagaaaa agggatggaa gccgatattt ttgcggtctc tgcctcagat gcagataata
    25441 atttagaatc taagaatgtc gatgtattat tattaggtcc tcaagtacgt ttcatgaaag
    25501 ctcaatttga acaaaaactt gcgcctaaag gtattccatt agatgtcatc aatatgcaag
    25561 actatggcat gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa
    25621 tcaagtatgc agttgggggt ataacaacgt ggaagatcaa caaaatctag aagccatcat
    25681 ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc
    25741 taaaatcggt gattttgagt tagcggatag gaaaatcgct gatgcagaag aatcacttat
    25801 acaagcgcat catgcacaaa cagacatgtt gacacaagaa gcacaaggaa atcatatgca
    25861 agtgacatta ttgactgttc acagtcaaga tcacttaatg acatctattg cgtttactga
    25921 tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac agcgaataaa tacaacttct
    25981 tttttgaacg gaagaagaaa aatttttgaa cggatttttt attttgttat aaggagaact
    26041 ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc aagttggatg
    26101 gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt agttgctttg
    26161 cgtgatgctt ttatcggcac actgcccgca acaatggcgg gatcagtcgc tgtaatgatc
    26221 aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga cagtttagca
    26281 gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg
    26341 aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta taatcttgcc
    26401 cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac cttgattcaa
    26461 ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc ttcaaatgtc
    26521 gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac actgtcagtt
    26581 ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt tacagttatg
    26641 atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa cattacgatc
    26701 aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat tttaccagcg
    26761 acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt agctgatggg
    26821 caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg attatctcaa
    26881 gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt ctttggtatt
    26941 catggaccaa atgtattggc accagtgcta gagggaatct ggggacaagc acaactaatc
    27001 aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc cgtgttagat
    27061 gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg
    27121 tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt ctctaaacgt
    27181 gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa tatcaacgaa
    27241 ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc attcttgatt
    27301 gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct agtcgctccg
    27361 gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt agcaacagga
    27421 gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg
    27481 gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca acaataagat
    27541 gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc taggaggaac
    27601 tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat tgttagaagg
    27661 atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca agctgttctt
    27721 tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc
    27781 acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa tggtttaaaa
    27841 agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg gttctttgat
    27901 cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta tggtcacagg
    27961 tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa gagaagaagc
    28021 aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc atcgtttttt
    28081 tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg caaatcagta
    28141 actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa taaccgggct
    28201 cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac atcgacaacg
    28261 aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt
    28321 cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga tatgctttta
    28381 ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa
    28441 gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg aagtaagcta
    28501 gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc ttttttgtag
    28561 gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg attgaggaaa
    28621 tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg cgagaacgtt
    28681 ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag gatcggctaa
    28741 caagtcaagt catggcgacc ccttttccag tgaattttta cggacaagag tacctaatgg
    28801 ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg atcaatcaga
    28861 tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct tatctggcgc
    28921 cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat aaaatcagtt
    28981 atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta
    29041 tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg ccggaaaacc
    29101 aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag caaaaaggca
    29161 attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat atcgtttatc
    29221 agcttgcaac gtcttccttt attattgcgg agtggggatt tttggatcat gatgcttttt
    29281 gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt gaatattcat
    29341 gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct tctgtaatga
    29401 tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa aattatccat
    29461 ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat gcgaaatgga
    29521 atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca aaagtagaac
    29581 aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta ttattagggt
    29641 atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa gaatatgaag
    29701 aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat ttttgataaa
    29761 aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg catttgaagg
    29821 aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt cttttgtttt
    29881 agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc gatttttttt
    29941 agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca ttggaaagga
    30001 cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact agtggaaacg
    30061 ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa aaaacaaaca
    30121 agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc aatcgcctcc
    30181 taagcgatag gtcgctggtt caattccagt caggaacgta taaataaccc ctgtatcctt
    30241 tgcggtacag gggtttgttt tgtatttgtg ccaaaaatgt gccaaactag ttatcatttt
    30301 tatataaact atcgaacaca gtattgattt tttctttatc ctgatcttca agctctcgaa
    30361 caatatgaag ataagtagac attgtagttt ctaaactgct atgtcctaaa cgtttcgata
    30421 cgctaagtat atttactcct tgataaagta aaatagatgc atgtgtatgc cttagtccat
    30481 gcaaagtaaa ttgcttttca atccctaatt cttttaattt tttgcgcaaa tatttgctga
    30541 cggcgtttga tgaaactaat ccatttttta agttgaaaaa aacaaaatta tgagggtttt
    30601 tgatttcaaa attctcgaat aattcttttt gattgatttt aaactttttt aagagatcaa
    30661 tcgtatgttt atcaatggaa attttcctat ttgaagtttc gttttttgtt tttccccatt
    30721 catttaattt ataattccat gttttattaa tagtgatgat ttgatcttcg aaatcgatat
    30781 cttcccatgt taatcctaga agttcagcaa atcgcattcc agtggcacca gcaactaaca
    30841 cgagcatagg agaagagtac tgcgcagaaa ggttttcttc tacgagtttc attaaagatt
    30901 taaattgatc ataatctaaa tatttatctt cttttggctt caatgaatct tttcctttga
    30961 ttactgcttt tctagttgga tcaaacggaa ttaatccttc ttctacggca tctttcaacg
    31021 acgctctaat atggttatta aacttaataa cggtagattt tacatggtct ttagcatact
    31081 tatttaaaaa gcgttgatat cccactctat ctaaatcaga aatcaatacc gctggcatat
    31141 atttctttat gttcattaac gtatcttcgt attttctata agtgatagga gatactgttc
    31201 cttctttata aagttgcatc caatcctcaa agtaatctga taataataga tttttgcgat
    31261 cccctttaag gcctttgttc aagttatatt ctaattcgtt ggcggcatct tttgcttctc
    31321 cttttgtctt aaatcctgat tttcttatct tcgtgtattt tccgtcatct tttttgtatg
    31381 aaatttcgta ttgccaacta tttccacgtt tgactaatct tgccataatt gaatcagctc
    31441 tctttctctg atacaatagg aactataaag aagcccattg tttgggtttt attatttttt
    31501 gcacatcctt tttgcttgcc ggcgcaagga tgtgctattt gtcgttttaa tggtataatt
    31561 ggatgtgtat tagatcatcc aatcagaggt gtttattatg attaaagtga tttttaaaag
    31621 aataactgaa cttccagagt ttagattatt tgttgattat tggtatgtct ggttttttat
    31681 tgttgcattg ctgataggtc tagatgtcat attagaaaga aaacttaagt aagagttgag
    31741 ttaataaatt gaattcctaa atgagatatt ttgtagccca tattttgttt gccgacatca
    31801 cctaatacta tggagctaat cgttttttct aacagaccaa aatcagataa tctatttaag
    31861 ctaccatcta ttagctcttg tgaatagcca gacatgctga tatttccagt gaaattttcg
    31921 tttccacgac tcattaagca tgctaacaat tgaatctcaa tcgatgttaa ttgattcagt
    31981 acaagaagat aatactcctc gttatcccat gaagcgctgt taattcgaag taaacaattc
    32041 ttataaagat ttttatacat atctatttta ttttgtgcct ttgatttttc tacctcgtca
    32101 tttatccctt cgaatattcc aatgagttgg tccttgttct ctaagttgaa aacatcttct
    32161 ggaatttgat cgcgaatgtt ttctaatgat tcgtttaaat ttttgtagaa tttctcgatg
    32221 cgtttgaaac gcttctcgtt tttagaccca aaataaagtg actgcacaac gccaccaata
    32281 taaggaattg ctccaatacc gccttcaact attaattcaa gatattcttt tttggttagt
    32341 ttttcattgc tattatccat agttacctcc tattttgtca tttcctttac aagcattgtg
    32401 actcctgatg gcactctttt gtataggttt tattattgtg cacaatctaa ctcttagtgg
    32461 ggcgtggttc atttattttt tacttacttt actattaatc atatcccatt gagcatagga
    32521 tttttctatg ttcgatcctc ttgaactaat ttctcctgta aaagttactt tatcgccctt
    32581 tgtaaaggta tcagcattgt ttaagtcctt cgcaaaaatt acatatgaaa ctttaggtgt
    32641 tgcagaaata tcattccaac ttttatcatc gtactctttg tctgctataa tggctattct
    32701 agtagtcatt gattcgataa cagttccttc aaaagtatat gttttaccac taatatattt
    32761 agtaaaagct gtattttgct cagtagctgg agtaatagaa taataactat ctaagaaagc
    32821 accccagtca tgattatttg tctctaaaaa ttcttcaaat tgagttaggc ttttattgct
    32881 gctgttagat gtagtagttt gagtatcaga tgatgagctt tcctgtttgt tattgtcatt
    32941 tgcctcgttt gatgaggaaa gcgagaaacc tacggtagta aaaataatcc cgataataag
    33001 gagaatcata ggtgttttct tatttgtttt tttgataaat cttacaatta acatgattaa
    33061 acctataata aataaaacaa taccagtaaa accaaatata ccgtccatta atagatcctc
    33121 tttctctttt gatattgaat attataaacc aagcaattct tttttcttaa aatcgaattc
    33181 ttcttgatta ataatccctt catctaacaa ctctttatac tttttaattt catctgcact
    33241 tgaaatttgt gtttctttgg aattattatc taatatagat tgttgcttct ctatatattt
    33301 ttttaaattt tccatttcat tccaatattt ttttgtaaac ataacggtgt tttcatcttt
    33361 cgtggccgct aatatacctc cgcgactttc gtttccgcct agcaaggtaa attgtatata
    33421 accattactc atacctggtt tttttaattg tacggctgat atatttttaa aggggatagt
    33481 tttttctcct ttaatacctt gattaactaa atttataaat ccttttctaa taattgaaat
    33541 agaatcatca gttactttaa tcaaggtttt tcctggagat tttattaaaa tctctttttc
    33601 cattttttct tcctcatttc tgtgatatga ttttttttgt agaagctcat atatgaggaa
    33661 agagcccgtg ttgcagcacg ggctttttta atgttttgct gaaatcggtt tttttaataa
    33721 ttcttgatgt attcatacat attgccttgc gtaagaatat tttttcttaa aatagcattg
    33781 gcagacagca taacaagtgt atctgagctt attctgaata gaataatact ccataaattt
    33841 ttcgagatta aactgagatt catcagtcag ttcgttctca atatagatat ttaataaaat
    33901 tagaatagct attttatctg cttctgtttc aaattttgag tgaaaagttg tagaagtatc
    33961 gtacaaaact gaaaattcaa aatgagaggc gctgaaatgt gcaagttcat gagataaatg
    34021 aaaggcttct gcagtttcac cgtatagatt ttcattcaaa aaaatgattc tgggttttgg
    34081 atagtagaaa ccaggctctt tcatctccat ataaactact ttcaaattgt attcgctcaa
    34141 catttctttc aatttcaaat acatacaaac catcactcca actattcatt ttcctctaaa
    34201 gctttagcaa ttgcaatcgc tttacgcatt gtctccttag atatttcttt tccatcaaaa
    34261 gaaaaaacag tatcgtcttc tgataaatcc acatgtttag gggtttctct ttcttctcta
    34321 cctagaaggt agtctacaga gacatcgaaa tagtcagcaa tccgacttaa ttcatctgaa
    34381 ttaggagtat tatttttcca ctttgctaga taaccgttag aatatccaaa attaatttct
    34441 aactgccgta tagatatctt tttcctcttc gccaagtctt ttattatttc ataggtattc
    34501 atcgataaaa caacctttct aaatgtttac aaaaaaagtt tagaaaaata cgcagaaata
    34561 atttgactaa ttctgagtaa tgagctatac tatatcttgt aaacagattt aacaactaaa
    34621 aagacaacaa aaaacactat tgatatgtaa atgcagaccg ccaagaaagc tttaaaatca
    34681 atgtttttat gtcttattta attatgctct tagtatagag tattactcag atactgtcaa
    34741 ttgaatttag aaaaaagttg ttaaatttgt ttacgaatat aaaagaaagg aatgagaaat
    34801 atgaacacac cgcaaatttt taatttcgaa caaaacgaag ttcggacaat tttagtaaat
    34861 gatgaaccat attttgtagg caaagacgtt gcgagtgttt tgggttattc aaacactaaa
    34921 gatgctttgt cgcggcatgt agatttggaa gataagatgg ggtcgcgaat cacgacctca
    34981 ggtcaatcaa gagagatgac aatcatcaac gaatctggtt tgtacagttt aatcttaaaa
    35041 tcaaaacttc cctctgccaa aaaatttaaa cgttgggtaa caagtgaagt gttgccagca
    35101 attagaaaac atggaggtta tctaactcca gaaaaagtag aagaagcttt gcttaatcca
    35161 gatacaatca ttcaattagc aactcaacta aaagaagaaa gaactggaag attaatcgca
    35221 gaacaaaaga ttgcagagta cgaacctaaa atctcctatt tagatagcat attatcttct
    35281 acagattcag taacaattag tcagattgca gcagattatg ggatgtctcc acaacagatg
    35341 aataaattac ttcataaact aggtattcag aaaaaagtcg gtaaccaatg gttattgtgc
    35401 aaaaaacaca tgaaccaagg atacacaaaa tctcatacaa ctgagatccc gaaagccgat
    35461 ggtggcacta aaattgtaat gaataccaaa tggacacaga aagggcgtct atttatctac
    35521 gaattactaa aaaaagaagg atattaccct caaatggatt tagaggaaat tggttagaaa
    35581 ggagttttag tatgactgac attgcagaaa tcactcaacg agatagagaa aaaatcaaag
    35641 aatatgtcga aagttcaaag ttcttaactt acaccatgct tgctgaaaga tttggaatta
    35701 gcaaaagcta cttatcttta attttaaacg gtaaaaagac ttctgcagaa gcaaacagaa
    35761 ttatagattc gattatcact atgtacgaat tgtaaaggga ggaacagcta atgcaatatc
    35821 tagaagcaaa aatcccaatt ccagaaggct atgtaattat ctcccaagtg gattatgagg
    35881 agttaaaaaa agctgatgat actggtagat ggatgacgtt gccagaagtg ctagaacgga
    35941 ttaacagaaa atatgattgg tttacttcta gagttttaaa gaacccaaga tatagaaata
    36001 ttatcgatat cgagaaaaac aaaaatggat ttgtctatta tccagttgaa ggaagagaca
    36061 catatctatt tttaagaagt aaaacacttg aatttttaga aacaaatttt tcggaaatct
    36121 taaggaggca agcggatggg aaaatttaat agagcgctag tgttcagcgc accgctaatc
    36181 atctacgctt taggactttg gggaagcagg caagcgttga taggaacgat tgtttacatg
    36241 gtttggattt ttatggggct tgatgaagct gaagctgagt acagagcgaa aaagccaacc
    36301 gagggggctg actaaaaatg aatagaaaag agaaactaga atgtatatta ctattactca
    36361 gtttaattct ttcactaatt tctctattgg gaagtttgta tttttgatat caacaatttt
    36421 gtggatttgg gttctattta gagtaaatat catttttgtt tggtgattaa ggagtttatt
    36481 aaaattaatt ttccctactt caaatgcaag gtaacaagat atagcacttt taggaggaat
    36541 atttagcggt acagcttcag agtatattct attatcatcg actgatcctc ctttagcaat
    36601 taatttctta aatggagtag ctgaccatct gcggtttaag ttagcggatg aaagttctaa
    36661 attaactaac atagctggtt ctgttgaata gttagaaatg attacttttg tataaatcat
    36721 atctgcagcg aagtaagaag cattaagttc aacttctagt tgagggcgca ttccttttat
    36781 ttttatccct gttaaaaacg tactcaatat aaaacttatt attgacatcc attgaaaaat
    36841 tgttaagtga aaaaaattca aaataaacac caccagtttt taactaaatt ataccaaaaa
    36901 ggagagaaga aataatgcaa gaattagtaa ttttgaaaaa taaagaagct gtgactacga
    36961 gcttacaagt ggcagaaagc ttcgagaaaa aacatcaaca tgttttaaga gatattgatg
    37021 cactaaaaaa agatgtgtcc aattttggac agatgtttgt agaaggtaat gaaccagatt
    37081 catatggcag aaatcgacga gttttcttca ttagtagaga tggatttttc ttgctggcta
    37141 tggggtttac aggaaagaaa gctatctact tcaaacaaaa atacattgaa gcattcaacg
    37201 aaatggaaga tgttattcgc aagaatactg ttcctcaaac aattgaagat atgatgatct
    37261 atcaattaga agaaatgaaa gatgttaaaa aagatgtttc catgcttaaa gatactatgc
    37321 gaattagcgg acaacaagag tttgaaatta agcaaaaagg aaatatgaaa gttatgggag
    37381 ttctaggggg aaaagaaagc cgagcttatg aagaaatcag caaaaaagta ttctcaaaat
    37441 tttggtctga atttaaacgt accttttcaa tcccaagata tggcgagtta cctcgtaaga
    37501 gattcgatga tgctgtttca tttattgaaa tgtggttgcc agaaactgcg atccgtatgg
    37561 aaattgatca actgaacaga caacagagac tttttggtga tgacaatgaa tagagctgaa
    37621 gcgctaagaa tagggacggc aattgctaat tgctggtgga aatactataa accaatcatc
    37681 ctaagccaac aacatattga caagcaaaaa gcatggcaac aaataaaaaa gtgactccgc
    37741 cggcaagcaa agagtcacaa agaaaacaca tcataaggag attttagcat atggaaaaag
    37801 aactttccac tctagatcaa tatttgattg atcctgattg gggcaagccg aaaattgagg
    37861 aaacaagtgg tcgaaaaatc agacgaaatc ttttgacgaa tgaagaacta gcttgggatc
    37921 aagatgattt aggcaaccat gtaactattt gggatcatgt ttatcttatc catctatcga
    37981 agcattcgaa taaacctgaa tatatttacg tcatcgaaga tggcttgatt gatgcgctag
    38041 aagagtacga cagagataac ttgattgata tctcttatta cggaccaggt aagaaataca
    38101 ttgctgaaat ggaggcagaa tttgatgagt gaaatcaaag ggacaacgaa ctttgaaaaa
    38161 ctttttagtc gtaagttaaa taaaattctc aagaaaaaag gaaattttga ttatttatct
    38221 tgggctcacg cgtgggagat tatgaaaaag aatgatccac aggcaacggt aactattaat
    38281 gagtacaaac actacagggt tgtttctgga actcatcaag actttcttgt tgaggaatat
    38341 aaaccttttc ttatggatga aactgggact tatgtatctg tctcagtaac ggttaaagga
    38401 cacacggaaa ccgaattatt tcctgtttta gattatcgaa accaaccagt tgttaaacca
    38461 aatgcaatgc aaatcaataa ctcattgaag cgatgctttg tgaaagcatt ggctctacac
    38521 ggactgggat tatatgtatt tcaaggggaa gatattccaa caccacctag aatcgataca
    38581 aagaaattaa acatgctaga gacgattcta gaagctttca atgagcagat gggtaaagat
    38641 atgaccaaaa ccttaatcga atatgttaat gagcagacag ataaattagg gctcttagct
    38701 gataacgttg aaactattga acagttaagc tatgagcaat gtgccttgat ggagcgagca
    38761 atagcagcta agagaaaaga attagataag aagtgatatg agtgtttaaa ccattaatcg
    38821 attcatattc agcggttctg aaaaagttca aaggaaaaga cataagcgca accatcaatg
    38881 aggaagtgaa cattgatcga ctaaagacga tgtatgacgg ctacgatggt gatcgagtca
    38941 ttgaaattcg ttttattgat cctagacgtt tcaccgtaca gcaacgaaac ttcatctatg
    39001 cgctgatagg cgatattttt atcgatacag gcatgccaac ggacttctgg aaggaattct
    39061 tctactttcg ttttgaaggt gtcacagggc gcaaaataag cctgaaagac gaatcgaata
    39121 caactgtgag tgatgccaat gtcttagcaa atatcatctt agatttcatc tttgaacatc
    39181 atattccttt caaagaaggc tatgagattt tacctgcgaa tcaagaatat tacttctaca
    39241 aatgcatcac aaaaagagtc tgctgcatct gtggcaaaac aggagctgac atcgatcact
    39301 ttgacaaagc gctaggaaga cgaaagcgca aagaagttga tcatgcagag tacacatttg
    39361 cagcactctg cagaatccat cacacagaga agcacaaaat aggtgtgatt aatttcaaaa
    39421 ataaatatca aatcaaaggg atcaaattaa accagaaaac aatcaaaaag ttaaatattg
    39481 gagggtaaaa atgacagaac atcgaagtta ttatgcgatt ataccagcca acgtaaggta
    39541 cgacaaaaga cttaaaccaa atactaagtt gttatacgga gagataacgg ccttgtgtaa
    39601 tgaaagaggc ttttgttggg caggcaatga gtactttgca gatttatatg gtgtgaataa
    39661 agagaccata tcgcgatggg taagtgattt gattaagttt ggatacttga atcgggaaat
    39721 catttacaaa gagggtacca atcaaataat caataggtac ctacgaatta atcaataccc
    39781 tattgacgaa aaacgcaata cccctattga cgaaaaagtc aaagataata atacatctat
    39841 taataataca tttaataata caaaagaata tataagagag ttaccgcctt cgaaaaaatc
    39901 gaaggctaag cccgtccgtc ataaatacgg agagtataaa aatgttcttt tgtcagatga
    39961 gcagatggag aaactcaaaa cagaattccc taatgattac caagagcgaa tcgaacgact
    40021 gtcagagtat tgtgaatcat ctggtaagac ttataaaaac tatttggcaa ctattcgaag
    40081 ttgggcaagg aaagaaaaaa gtgaacctaa gaacgcaagc agtggataca agcgcacagg
    40141 aagacgagag aagcttcctg aatgggcaat cgaccaagaa gcctatctca agaaaaaagc
    40201 gctagaacga gctaatagac aatcaaaagc accattctaa gaggtggaaa aatgaagatc
    40261 gattatctag aactaattaa tgaaatagcg aattataaaa agggcgagga attagacgtc
    40321 ctgagagacg tatatgatca actcgaagaa gctggaattg aaggaattaa gaatgatcgt
    40381 tcgagttgga gtaaactcag atactatttc gcactctata tcgatggaac gcaattaaga
    40441 aatttagcat ataccaaatt actattcatt gattgtgtta aaggcttgca aaaacatctt
    40501 aatgaacttg agcaggtgta aacaagatgg acctaaagac atttacagca cagatcgaat
    40561 taatgcatca agaagcttta agacaaagtg tgtcgtacga agacaagtgg ctcaacacat
    40621 ttcatggcgg acgtgagagc gcacttgatc aagtactcaa attactgaaa ggagaatgtc
    40681 gggatggata agaaagcggc aatgcagcga attatcgaat tgacttattc agaagattgg
    40741 caggaagata aaaaaatagt tgcagaagtc caaaagctcg gtaaatcaat gtggactgaa
    40801 aagcccaaac ggagaacgcc gagaaagatt gcaatctggc atggtgatcg aattctagta
    40861 acgggtaccg ctaaacagtt atctgaaatt actggtctga gcaaaaatat tatttgggat
    40921 agagctagga gcttatggat tgattcaaaa ggaagacagt ttaggtatgt ggaggagaaa
    40981 taatggacga actaatcgcg aaagtagagc agtgggcaaa agataaggga ttggatcaag
    41041 cggatttcag caagcaaatg ttaaaaacgg tcgaagaaat cggggaagtt gccgcttctc
    41101 tagctagaaa agatgaacat ggtttaagag acggaattgg cgatgtagta gtaaccttga
    41161 tcatcttggc aatgcaaaat gatatggatt tatacgagtg cttggatttt gcttatgacg
    41221 aaatcaaagg tcggactgga aagatggtca atggtgtttt cgttaagtcc agtgacctga
    41281 aataattcca ttaatggaag aaataactga ataggaggat agacatgatt gatgcaaaac
    41341 aagtgcaaaa acagaaagat ggaatgttga tgtttgaagc atatgttcta ccatttctta
    41401 atcaatttga agttttggaa tgctctgcca gcggagaaga attggagtat gttgtaattc
    41461 gtgaaaccaa agaaaacgtt cagaagctaa acgaatttct ttgcactatt aactgttggg
    41521 atatgatagc tccaggattt ttatgtccag ctatgggaga atttttggaa tattgtcgtc
    41581 ttgaagatgc aggcactttg gatttagctt atctggtata caactaccta aacatcaaca
    41641 cagatcattt gtggtttggc actgccgaaa gaaaatgggt agtccgctaa ccaccaatat
    41701 caatgaaaaa aggcggtttc ctgaaagtgg aattgccgcc attgctgtaa ggttctttac
    41761 gcgactcaag gtcacagaaa tataaatgca aatctctttt gaagtgatcc aaagctctcg
    41821 gaaaatggaa gttttttgtt tcaaagaata aatgagattt gcgtcaagaa cctgagagtt
    41881 atttccaaaa aagcttccaa agaaccgaat gagctaagga catcatgtgt aaacacataa
    41941 tatagctctc ctttcacggc taatcataac atgaaaaata ttcgatgtca gtaggaaaaa
    42001 aggtgaatta tgaaaagaat attaaattat cccggcagta acagattctg caattaaact
    42061 ccgaagctga tgtatcagta tcaactgtag aatattatga gcgtggagaa agtcaagcag
    42121 tcacaaatgt aaaaatcgat aacgtcaacg gtcttatctg tttagatgaa ggcgatagta
    42181 tgaatgaaat tgctgcagac catgaattat cagaaactgc tttaattgat caaatcgaat
    42241 tagctagaca atactttgtt ccatatgcag atacctggtc caagcataag aatgacatca
    42301 tttttccaga ggaacaatga gagcaactga tccagtaatt atccttgagg aagccaaatt
    42361 tatttggact cacgaagaga tagagcaagc acgcttgctc ttttctcaag gagttaagcc
    42421 gagcaaagta gctgaaataa tgggtcaaaa gattcttgat gtcggattgc ttttgctcca
    42481 tctagcagaa aaaaatttga tttgaggtga aaatgatgat tcaacttgca ggcatccaaa
    42541 cagggaaaat ttatttttct ggagaaagca aaagcgaagc caaccaatgg ctattgaaaa
    42601 cttacacgaa caataagaag ctaaggaaaa aatatcctga tttgttgcta aaggatgatc
    42661 agattatgcc ggaaccgatg attttgaccc gtaaggaggg aagttgaaac taaaaaacca
    42721 gccgtgtggg ctggagttgc tatttctttt taataatgag tttttctaaa tttaattcat
    42781 tctcacgagt ttgcgtccaa taatcagaat aatattttaa ttgtttaaga gtaggaatgt
    42841 cgtcttcggt aaaattagaa aataattctg gagaattatc tttttttaat tgttctaatc
    42901 ctgattcttt aaaacgttgt tcaacgagtt ttactaaatt gttgatattc atgataaatc
    42961 acctttttaa atttaatttc gatttgatta atcgatacaa gtattatatc aaataaaaaa
    43021 gctgatttag ggggtcttac aaaaacacta aaattttttc aaaaagagga ggaacggtgg
    43081 tgaaagtcag agaacaattc aagatagtga gcgacgaaga taatgtttac aaaataaaga
    43141 tcgttaatat taatgaatat cgagaagcag atcacaaata tttggttgac ttatacatga
    43201 acgataaata tttgggagaa ggattttgtg gagattcact ttttgatgat gagcgaattt
    43261 tgaaggagga aacagaatga tccacgaact aaaaatacta ccagaatatt ttgaagcagt
    43321 gacgagcgga cgcaaacaat tcgagatccg caagaatgat cgtgacttca aagttggtga
    43381 tcagttaata ctacgtgaat ataaaaaata cattaaacat gaattacctg gactaacgag
    43441 accaacattt agttacactg gtgactcata caaagcagag atcacttata ttactgatta
    43501 catgcagaag tccggatatg tagtgttggg gattaaaggg gtgaattaaa tgaaagtata
    43561 catcttagct atcacagagg gcacatggat gttcccagtc ggttccggaa aaatctataa
    43621 atcaaaaaca gctgcttata aagcatttga aaaatacaaa aaagaaaacg gtggcggcac
    43681 taacgcaaag attctagtgg ctgataactg gcatgaagaa ggtgaacgta attgatttac
    43741 ctgttcattt tcgtggtact aactattttt atgctagtaa tcttattcaa aaaaagatga
    43801 gtaaagacga acgaaaaatt ctggccaact tttatttagt attaaaaatc gaacaaaatg
    43861 tgttttcgga aaggagaatt gtatgggaaa aacaaaatca aagattaaga agaaaaaacg
    43921 acgtttggaa caaaaggcaa tccagaatgg aacggctaag aaaaaataaa aaagctaccc
    43981 ctttcgagat agcagcgcaa tactattcta ccataagggg tggcgtttgt gagatttcat
    44041 tggttaaaag attaccaaga actagatgag cagattcttt acttaaagtg gaatcttaat
    44101 aaaagtaagc ttgaattgaa tcgatgggtc tgtggtgatt tagcaaacgt ccgcatcgaa
    44161 aagaattcga gaacttcttc tttagaagag aatatccaaa agatagaaaa tgaattggaa
    44221 ttgttgatcg agcaaaaaga agaaatgtta ttgctgatag atagcttttc tggtatcgat
    44281 aatcaaattg ttaagatgaa atatgttgat caaatgagct tagaagatat tgctgaatct
    44341 gttgggtata gctcatcgta tgtcagacag cgacatgcag aaattagaaa gacattgaac
    44401 tttttagatg agtatgaaca cagacaagct gaccgattga aaaaagaaaa cgaaattgat
    44461 ttttacaata gcgagaagta caaagaacaa ctctctttat tctaaaatta cactgttcac
    44521 taaatgttcg gtcattgtag ctatgtaaac attgttagca gcatgatatt ctattagtgt
    44581 caaaaaaata tgaaagagcc aagatatccc aaccgtttta ttaattggta tctgtggctc
    44641 ttttattctt ttaattagta gttggcataa agttatttcc atttataaat tgatcagcta
    44701 aatcagcatc ataatcagcc gcgataaatt gcgctgctga tttaataaat gcatccatat
    44761 catgaacatc tttattagta tgtctacgaa catagtgcgt ttcatcatta ccaatccaag
    44821 tagcagcttt ggctaatctt tggagtttag gaaaatcatt caaataattt tcaattgttt
    44881 tacctaataa catttttttt attttatctt catcctctga atttttagat attgcataat
    44941 ctttaattag aaattctaga gcttttctat aaccaactcc agcaatttgt tccaattgct
    45001 cagatttagc ttgtgtggct tgagtataaa tatcaacaaa tgtaggagaa actttttcaa
    45061 tattttctgg taaatctatt ttaatcggcg gacggtaata atctttaatt aaaatatttt
    45121 cagaatatcc ttcatcttca ttaaacgtat ttataaattg taaactataa aatttgttgc
    45181 actcatcatt tatacactgt actaatacgc caaagattct atcattatcg aacgcactat
    45241 atgatgaaac cccaaaatac acatacggag tcattatatt tccgcaatgc ggacaactat
    45301 ttggcaaata aattgtctct gtattttgac gatcacgata cttaatatta acttcttgaa
    45361 gcattaaaac acctcgcatc ttttttataa atcataccac aaaatatctg ataggagagt
    45421 aaacaaatga atgattttca tgaagcggta cttagtattg aagtagaatc aagtttggct
    45481 aaagtttata agaaagcaat tgaagcagag aatagtccgt acagagaaaa ttggaatggt
    45541 aatcatgcac atgtgcaagt ggagaatgat gattacagaa ctggaatgaa tacgttagtt
    45601 atttctttat tgtcacacac tctacccaat ctgcaggaaa cgattgaatg gtatgagcga
    45661 atgggcgcaa aagtaatccg tacaaattac aaaggagaga attaaaatgg caatgatcaa
    45721 aattagaaca tccattagtg gaacagagta ttgggattca gaaaagaaaa ggacagtagt
    45781 tgttccgaaa gatcaagaac caaattttga agtaaaagaa gaaaaaggaa ttttgagtga
    45841 tggcaaatca tttgttgctg tcaatggcga actcatagct catagcagtg aaattcttga
    45901 tagcgatgga aacactgctg ctaattttga tggagacgaa gctactaatg atcagtccac
    45961 tgaagatacg gatgaactgg acaatatgac tgcaaaagaa ttgcgtgcat atgctaagaa
    46021 acatggtatt gatattcctg gtgctatccg tgcaaaaggc gatatcataa atcttattcg
    46081 tgaagtagaa tgaaatactg tcagtttgac ggatgcacga acaaaatagc aaagggtatc
    46141 tactgtactg aacacaagag atcaagcaga tcacgcaaga agaagcagca agcaaagtct
    46201 gtttatcatc atgagaacaa accattctat cgaacgcaag catggaaaga tatgcgtcaa
    46261 tttatttatg aaagagaagg tggtcattgt cagcggtgtg gtcagttcat atttggaaag
    46321 agagcacacg tccatcacat tgtaccaatc aaagacaacg aactgcttaa gcttgatcca
    46381 aacaatctca tgctattatg ttcaaaatgt catccaattg ttgaaaacga aacggaagac
    46441 aaaaaagttt ttccttcgta tttcaattga agccccccta ttcattttca aaattttttc
    46501 gcgtggggag atagggtagc ggggagtcac gcgcatcgtt aggtcaaatt tttcaaaaaa
    46561 caaagggggg tgtatacaaa aatgacgact aaagcgcaac gtaaagcgat tattgatgaa
    46621 aaagtaagtg ctgaaaaagc tcgtatctta gaaataatga atttgtctga tttgtacacc
    46681 atcactcttg atccattaat cgaatcatac ttggatattt ttgaaattta ccaacacaaa
    46741 tatttattgt ggaaggaaaa aggatttccg gagacgcaaa aattcacgaa taaatcagga
    46801 gctactaatc aatcaaaaca tccattggca caacaagtag aaacttgggc agataagaag
    46861 atgaaagctc tagatttatt aggattaaca aacaaagcaa aaactggtag actaattgct
    46921 ggaggatcga ccgcaagaaa agatgaagaa attacacgcc cagaagtaaa gccagtagat
    46981 gaactagcag cgcacagaaa taaatggcgt aagaaggcag gtgctgaaaa atgattgaac
    47041 ctggtgtaaa ttatgctgat ttatttgcaa aagaagtaag aaagaaacct aagaagtatc
    47101 ctaagaccgt tcgtttagca atagatcgct ggtatcagtg gaaaaaaaga aaagatattt
    47161 ggtttgatgt agatcgcgcg aatgaaatga tggattgggt agaatcattt attgttcaca
    47221 caaaaggaga aatggtggga aaaccgtttc ttttagaacc ctgggaaaaa tttatttatt
    47281 cttggattta tggttgggtt aaagaaaatg aaaaaggcca aattgtacgc gttactcgtg
    47341 aggcttatgt ccagattcca aaaaagaatg gtaaaacatt gattgctgtc ggttcattag
    47401 gatatgcaat gtacggagaa ggggcgttat cagttgattg ctatgcttgc gcatctgatt
    47461 ttgctcaagc gcaatatgct gccaaacctt ttgcagttac aatcttaaac aatccaatcc
    47521 tacttgaagg aactaaaata tttagggggc caaagggaac tgtttctagt attacttatg
    47581 actatattca tgaagatatg gcttactcaa ataaatttat tgttcaaacc aagaatattg
    47641 ataatatcga gggatccaat ccatattttg tattgaacga tgaattgcat aaacaggaga
    47701 aaatggaaca gtatgacaat tttaagtctg ctcagatatc tttaccacag ccattgatgt
    47761 ttaatatctc aactgctggg aaaggttcgt cgtctgttgg aatgcgtgta tatcgcgaag
    47821 caaaagaagt gttgaaacgt gatgataatg attcaaactt tgttctaatc tatgagccaa
    47881 ataaaaatta tgattggacg gatagaaaag tctgggaaat gtgcaatcct aactggggaa
    47941 tatcagtcga tttatctgct ttagaatcgg catttaaaac agcgcaacgg tcagctcatt
    48001 ccaaagctga atttctaacg aaacacttgg atgtatttgt gaacggcgcg gataatttct
    48061 ttgaacagga tcaagtagag ccatgtttag ttaccacaca agaacttggt gatttaagtg
    48121 gcgaaccatg ttatatcggt ttagatttat cacgtacacg agatttgacc tgtgtatctt
    48181 taaacttccc aacatgggat gaagatggta aagcggttct taaagtaaag caactttatt
    48241 ttattccgaa tgaagattta gagtttcgag aaaaagaaga taatgttcct tacagtgatt
    48301 tggctgaaca aggtttcgtt gaattttgcg acggaaaaat gattgatcaa gatcagattt
    48361 tgcagtacat agaggactgc atgaatttat atgatattca gcaagtgaat tatgatccag
    48421 cgatgagtga caaacttgtt gagaaattag aaaacttagg attggaatgc gttgaagttg
    48481 ctcaatatcc taaagtattg aatgctcctt ttgacgatgt cgaacggttg ttttatgaga
    48541 aacgaattca atttgataat ccattattcc tttattgcac tttgaacgtt gtagcaatta
    48601 ctaacatcaa tggacaaaaa gcgccaagta aacgtcaatc aaagaaaaag attgatgggt
    48661 tcgtggcgtt tttgtgtggt cataaggaaa cgatgaatca aatgacagat attgattcag
    48721 atgagttaga tgattaccta agttccatct atagataaat agaaaggcgg tgaaaaaaat
    48781 tgaaattacg tgatcggtta tcgaatgcag tctattcttt tatggaaaaa cgtggatata
    48841 tcgaggatat ttttggacac tacacacgtt atggtcaaag atatgtgacg gattcatcta
    48901 tcatggaatc ttctgatgtt tatgaattag ttcaagacat atctaatcag gtggcattgg
    48961 ccacaccaat tgttattggt cccgacggca acgaagtcaa agatcatcac ttactaaata
    49021 ttttgaagag tcccaatgat tatttgacag gattcgaatt taccaaattg gaaacaaata
    49081 ctttattgat caatggtgag acattcccat taacggatag ggatcagctt catttggcgt
    49141 acggtgtaac aactaaaatc aatgaacgac ttcaagaaga atttgaaatg aatggtcaaa
    49201 aaatacctgg tcaaatgatt cgacacatta agaacatcgg aactgattca ttaaaaggtg
    49261 ctggaataat tgatcttgca agaaacactc tagaaggcgt tctgagcgct gaaaaagttt
    49321 tgacggacaa atatactaaa ggcggtttac ttgcgttcat gcttaaacta gacgcccaca
    49381 tcaatccaaa taatagcgcc caaacaaaaa ttgtcaaagc tatattggat caactggaag
    49441 gaacgcaaaa tgagagtgat cattctgtta agatgattcc tttgggaaaa ggatattcca
    49501 tcgagacatt aaaaagtcct gttgatgacg cggcaatttt gaattattta ggtgtttaca
    49561 aaaaagactt aggaaaattt ctaggaatca atgttgatac gtatcaatcg ctgatgaaga
    49621 cagatattga aaaagcgatg atgtatctgc acaacaaagc aatcaaacca atattgaaaa
    49681 acaagagcga acattacacc gctctttttt ttatgcctaa ttctggctat agagtggaat
    49741 ggaaaattaa tattttggat tttgttcctt actcaacaaa aacaaatatt ggctacaaca
    49801 tcgttcgtac cgggattaca agtccagata acgtggcaga aatgcttggt tttcctaaac
    49861 agaatactcc agaaacacaa gctatctata tttcaaatga cttatctagg attggccaga
    49921 aaaatgcaac agatgattcc ttaccaacga atgatcaaga cttgaaagga ggtgatggaa
    49981 atgaagaaga aggaaattcg cacgattgac atcaccaatc tttcaacacg ttccgatgaa
    50041 gaaactcata cgaggaccat tagcggctac gctgctgtat tcaacagtca aacactttta
    50101 tgggatgact taagcgaagt gattgttcct ggtgcattct caaaggcaat tagtaattcg
    50161 gatgttcgtt gtttgttcaa tcacgattgg tccaatgtgc taggacgtac taaaagcgga
    50221 acccttcgcc ttgaagaaga tgaacgtggt ttgaaattcg aagttgattt accggacaca
    50281 acggtagcaa gggacttagt taaatctatg gaacgaggag acatcaacca atgtagtttt
    50341 ggttttgtgc cgactgaaga aacgtgggac tacaattctg aaccaatgct tcgaacaatc
    50401 catgaagtgg aattatacga ggtttctatt gtgcctttgc cagcatacga agatacagaa
    50461 gctgcattaa gaagtcgtga tgaattagaa aaaaacgtcg aagaaagaaa aaaattaatc
    50521 aaaaaaatta atcaagcgct agaagcgtag gaggaaaaat acatggataa agaattattg
    50581 aaaaaaatga aggcacgtcg tgagcaacga ttgactgaat tacgtgaaaa agttgaatca
    50641 ggagaattac gcgaagcaga tttagaagcc gtgaaagaag aaattgacag tgttattgat
    50701 gaattgaacg gaattaaaga cgaattaggc gcagattctg gaactgatga aacagacgac
    50761 aatacagatg atcaatcgaa tagtacggat tcagacgaaa gccgttctgg tgaagacaac
    50821 gatcaagaag aagattcaga cagtgaagat agttcagaaa atcgttctgg aatgattact
    50881 caacagcaac gagatggatt acttggatca attaagaacg gattggaggc acgtgcaaaa
    50941 atgaccaaga aacaaaaaga tcaacaacta cgaaaagcat ttgctaattt tgtagctgga
    51001 aatatttctg aagcagaagc tcgagcttta gggattgaag ctggcaacgg ttcagttact
    51061 gtcccagaag taattgcatc tgaagttatt acttatgctc aagaagaaaa tttacttcgt
    51121 aaatacggaa cagtggtgcg aacatcagga gatgtcaaat atccaattct tgtgaagaaa
    51181 gcagatgcga atgtaaacaa gaaagagcgt tcaactgata ttgctgaaac agctattcag
    51241 tttgatgaaa ttttgcttaa tcctgccgaa ttcgatgctt tggcaacagt aactaaaaaa
    51301 ttattaaaaa tgtctggtgt tccagttgaa gatattgttg tggaagaatt gaaaaaagct
    51361 tatgtgcgta aagaaatcaa ttatatgttc aatggtgatg acgctggaaa tgaaaatcct
    51421 ggtgcattag ccaaaaaggc tgtagcattt aaaaaacctt tagatctaac tgcttcaggt
    51481 gctgggcaaa aattatatga tgcattaatc gaatttaaaa atacaccagt gacagaagtg
    51541 atgaaaaagg gacgctttat tattaatcga gctgctttga ctgccattga aaaaatgaaa
    51601 acagatgatg gatttccttt gttgcggcca ttcacacaag cagaaggtgg aattggttac
    51661 caattagttg gctatcctgt ggattggaca gatgcagcag ataaaaaggg tgaaccagac
    51721 acgccagttt tatattttgg cgatttttct gcatttaaaa ttcaagaagt tattggtgcc
    51781 ttggaaattc aaaaacttgt tgaaaaattc tctggtaaaa atcaaattgg attccaaatt
    51841 tacaacttgc tagatggtca attggtttat tctccatttg aaccggcagt atatcgctac
    51901 gaaattacaa aaccagttgg tggttaagat ggaagagcaa actaaagaat tgtctttaga
    51961 ggaaaaattc aaatcacata ttcattttga agagggcatg gatgattctt tgctctcttt
    52021 ttatttaaat atggcaaaag attatgtcaa aacagcaact ggtggccaac aagaatatct
    52081 tattttgatg gttgccggca ttgcctatga atatagagtt tcagaggatg aactcgacaa
    52141 agctatgaat gccatgacgc catttatcgt gcaaggagcg attcaaaatg ccgaagagac
    52201 agaccaataa tctcagatgg aaagccgaat tgctagacat caaaacagga acagacggaa
    52261 acgatcgtcc aactacaatt tacgaattta agcgtctaat attctatgaa gaactcggtg
    52321 tgacttctca agaaaaatat ttatcacagc aagccaagac agacgttgtc agacgaatta
    52381 aagtcagatg ggataaatcc atcacagaga aattaagtgc gctcaaaatt gattctgtaa
    52441 cgtataacat tactcgcatt tatacgaatc ccgatgcaag agaaatggag ttgagtttag
    52501 cttatgtcga ttagctttga tgaattgaaa acagcgctga aatcaacaaa gttagcagtg
    52561 ttcagagaca aagccagatt agggacgatg tatccataca ttgtgtactc aaatgtgagt
    52621 aacagcaaaa aaatggcatc cggtaaagtc tataaaaaat taccttatta ccaagtctct
    52681 ttttttacac ttggaacaga acaggattta gttgttattg aacaagcgct gcaaaatgct
    52741 ggcattccat attcagattt tacaggtata caaggtgatg agaacgacga taccgtaacc
    52801 aactattaca catatgtgag gtgtgtggaa aatgccaagt aataagaatg gtttttctga
    52861 aatatcggat tatttaggaa acctttcaaa agttgatccg aaaaaattat cgttggaatc
    52921 tttagaagaa gcagctaact tttatctaaa gcagttgctt cctaatattc ctaagtcgct
    52981 cttaaaaaag aaacatatgc gcgatcaaat aaaagtagta gttgaaaacg atcaagtgaa
    53041 agttcaattt gaagagacgg ccttttactg gcgctttgcc gaaaatggca caacaaatca
    53101 acgtgcccaa cattttgcaa gtggtacgta tgaacaaaac aaagaaaaaa ttgaagaaat
    53161 tatgactaga aaaatacttg atttatggga agggtgattt taattgggaa aacaagatac
    53221 tttttatttt gaaggattgg acgacatttt aattgcgatg atggcaacgc cagattctgt
    53281 tggaactgca ccgacttata gtgaagtttt acgattgcca attgccacaa aaatcggtgt
    53341 caaagggaat ggaacagcat tagaaaaatg ggcatccagc aaaatgtttc gtcgtgtgtc
    53401 gcgagaaaca aaacatgaac ttgcgttgga tcacgtgggt attccgatag aagtgatgga
    53461 tgaaatcaaa ggattagttg ctaaaagcgg agtaacgttt agcaaaaata ccgcacgaga
    53521 atttccgtac ttcgcctttg gatttattgg aaatattgaa aatggcggta aaaaagcagt
    53581 ttggtatcct aatacacaac tatccaatgt gattgacgaa gagtatgcca ctgcagagga
    53641 agaaacaaaa attgacgatg taactgcaaa tctggtttcc atcggactga aatacaataa
    53701 tgtgatgtat gcaagttttg attcaaatag agacggagct tctctagaat tatttaataa
    53761 attcattgct caacctgtat acgatgaaga acagtggaaa acattggcta aagttggagg
    53821 tgcgggttaa tggctcggtt atctgattat ggaattcacg ttgaagactt aaaaaattct
    53881 gctgttgtaa ttattcaagg cgcagaattc cctatttcat ttactatgca aacgatggaa
    53941 tttatagcag atgtgtatgg tggagattat tcgcaatttg aatctgatat gaacgccatg
    54001 ctatctaaaa aagaaggaca aatttcttct gctaacttat cgcctagtga tctaaaaatc
    54061 atgcgtgcct tgatttatgc catgctgcgt actggtggtt tagaggaaga tccagaaact
    54121 attttcaaat ttttgggaat gagtggagag gtgttgtctg cttatagtac ctgtatggaa
    54181 atttttgcta gccagacatt tcaggtggaa gacctaaaaa aatccaagaa gccacaagac
    54241 tttcaaaaag cgcaagcaaa aagaaagaaa aacaaaaaga atcggaagag ataggaactc
    54301 cttggagttt ttatatttac gttgctctca ctctattgaa ttggagtgag agtttctttt
    54361 taaagtctac acctaacttg tggctcaaat cttatttaca gtggttacaa caaaacaccg
    54421 attttgaacc acctcaatct gtaactatgg ataaatcgcc ttggtggtag aaaggagcgc
    54481 taagataaat ggctggtaaa gaatctgatg tcgttcttaa ttttaaaacg aatggcgaag
    54541 tcagttattc gaaaacaatc aaagaaatca acaaagaaat gaacttagcc gctgccgagt
    54601 acaaaaacca agtgtctgcg atggacaagg atgcaactca aacagaaaaa ttgcgagcgg
    54661 ctaaacaaaa attagaaaaa caattaggat tggctgaaca acgatctcaa atgctgagag
    54721 aggaatatga aaaatctgtc aaagaaaccg gtgaatattc tgctgaatca gaaaaacttt
    54781 ataaacaatt actcaattct gaaaccggag aaaataaact tcgtacagca ttagaacaga
    54841 cgaacgatgc acttaaagaa caaggtgacg tttctgttga tacagcaaaa aaactccaga
    54901 aaatcgaaga aacaggtgag aaagtaaaag gcgttggtga aaaaatgtct gttggagtaa
    54961 ccgcgcctat tgtagcggca ggagcagcag gacttgcagc atttggtgaa gttgacgagg
    55021 cacttgatac catcattaca aaaaccggag caacaggtga tcaagctgat agactttcac
    55081 agtctttcaa aaacgttggt tcaaatactc atttaccttt acaaacggtt ggagaagcta
    55141 ttggtgaggt aaatacacaa tttggattca tggataaaaa actggaagat tcaaccaatt
    55201 atctcctaca gtacgctgaa atcaatgata cagatgtttc gcaatcagca atatctgctc
    55261 gacaagctat tgatgcttat ggactagaat atgatgattt gaattctgtc cttgatgtaa
    55321 caacgaaaac atcgcagaat actggtcaat ctgtagacga cttgatgcaa aaagcaattg
    55381 atggcgcacc acaaattaaa caattagggt tgagctttgg tgaaggggtc actttacttg
    55441 gacaatttga gcaatctggt gttgattcaa gcgcagcttt aagtagttta tctaaagcaa
    55501 cagtggctta tgcgaaagac ggaaaatctt taagtcaagg actagaagaa ctacaagaca
    55561 aggtaaaaaa tgcaggttct gaaacagaag ccatcaatgc agcagcagaa gtattcggga
    55621 ctaaaggcgg tcctagaatg gccgatgcaa ttcgtagagg tactttgaac ctggaagatt
    55681 tagcaaaaac tgctggagaa agtgggggag ctgtaggaga cacattcgat gctactctcg
    55741 acccaattga tcaagccgat caagctatga acaatgcaaa attagcaatg gctgacgtag
    55801 gtgaagcagt ccaagtaagc cttttgcctt tttttgaaaa agcaacttcc ttattacagg
    55861 aattctctaa atggtggggg tctctagatc aagatacaaa gaattggatc ataacaattg
    55921 ctggtatagc ggcagcagtt ggaccagtac taatcgttct tggttcactt atgggatctg
    55981 ttacaaaaat cgttggggaa gttaaaagtt ttataggagt ttggcaagga cttgctggtt
    56041 tatttggaat gtcgggaggc tggtttgcat tagcagttat agctattggc gcattagtta
    56101 ctggactaat ttgggcatat aataacgtta aatggtttca tgatggagtg aattctttct
    56161 tcaaaggtgt ttcagatgta gctgttgaaa tatttaattt tttaagtggt tattttggtg
    56221 gatttttcga tggtgttata gcaaatttca ataattttta taatgctgga aaacgaattt
    56281 tcagtggttt tctagatttt atcactggag tatttacagg gaattggtca aaagcatggc
    56341 aaggtctagt tgatattttt ggaggtattt tcgatggtat tgttgctgta gggaaagctc
    56401 ctattaatgc catgattgga ttgatcaatg gatttattgg tggattgaat aatataaaga
    56461 taccaaaatg ggttcctggt gtaggtggaa aatcattttc tatttcaaaa ctgccttatt
    56521 tagcacaagg cggtcatcta atcaatggac aagcgattgt tggtgaagct ggacctgagt
    56581 tactgactgc caagaatggt aaaaccacag ttactccatt atcagacgaa gaaaaacgca
    56641 aagggatcag tggaagagtt tctggaggta gcattgaaca gcatatacac attggcaaag
    56701 tcgatgcaaa caatccatct gagttagatc gtatgaatcg taaatttgct aaagcaaaca
    56761 aacaggctat tcgtgatttg ggaggtgttc ctatttgagt cgacaattta tgaatccaga
    56821 tgaaccaaat tttatttgga aaaatcggaa tgcagtaatt gatatgaatt gcatcattga
    56881 ggcagagctt ccagatatta tgccgaacaa gcgatacgaa acttacacga ttcagggccg
    56941 aagcggagaa ttgacggaga cgtttgatga ctatgaacct tttgatttgg aaatagaagg
    57001 gatcactatt ccacattcga aactgagaga agtcaaacga tggctcactg gtaaaagcca
    57061 attaatcaca cataatgatc cagacaagta tctagaggct atctgcagca tggataaaga
    57121 agtccctttc gaaaacgagt ggggcttctt ttatacattc gatgttactt ttcgttgtca
    57181 gccactcaaa agaaaaatcg gtgaagcacc taaaattttt cattctagtg ttatggatat
    57241 ttttgatcat ggtgacgaaa ttgcacatcc ctatctggaa atagaatcga acggaggaga
    57301 tataacgatc gctgtcggtg ataaaagcct aacaatactt aatacgttag ctgcaacaat
    57361 cgtggttgat actcagcttg gaaaagcaat acaagaaggt ttgaatttat ttacgcgtgg
    57421 tgattggcca gtattgcagc ctgaatggaa tcaggtaaaa atatccggaa aatttaaaga
    57481 agtgcggttt tggaatagga gtgtgtatct gtgacgcaag aatttattta tgcttataaa
    57541 aaaatgccag atgatttgag cgtgaacgga gcgtcgttag ttgattggga agatttgcca
    57601 gaaatcaatc gtgtgctgaa tggccaatat cgtttttatg gtaactattc aagaagcgga
    57661 cagtatcgct cttatttaaa aaaaggaaat tttataaaag cgaaggtacc agatggatca
    57721 tggcaatatt ttgaaattta taatgtcaaa aagaatttga cctccgtttc tgtaacagct
    57781 cggcatattg gttttatggc caataagaac tttattgtca agtcgtttac ggataacggc
    57841 aatgggtctc agatcatgac caatcttaaa aacagtttgg catttgatca aaaatttaac
    57901 tatctttcga atgtagggac aacgcaccaa tttacagcaa gacaagtcgc gccagttgaa
    57961 gcaatcattg gttcaaataa cggtaatcaa aatttgactg gcgtgactag cgcggaactt
    58021 gatatggata actatgattt gaaactggta aaacagattg gatcagataa tggttttcgg
    58081 attgattttg gcatcaattt agaagcaatc gaagaagaaa ttgatgaaga atcaattgtc
    58141 aatagtttgt atttggttgg cggtgttcct gataacgatt atgatgaaga caaagaacct
    58201 atcgaatatg gttatttaga aattgatgga gtaaccaacg agaatcgacg tattgccaaa
    58261 cgtgaaaacg gagattgtaa aacagttgat gaattgatca aatggggcaa aaccttattt
    58321 gacaatgatc gtatccatga gccaaaagct actcatacag tcagtatggt ggcgttggaa
    58381 cacacactcg aatacggtga catgtatcaa gagctagcgt ctttacactt tggtgatgtc
    58441 gctcatgtta gggcgaaaga gctagatatc gagataaaag agcgtatggt cgaatacact
    58501 tatttcccaa cgcttgggaa atataaggat attgttttag gtaatgattt atcactgtac
    58561 acttcggcag taaatactca agcacaagaa ttgaaaaaga aaatcgacaa tcgaacagaa
    58621 acgttagttc aaaatgtgtt gaatgcgacg gcatggatca ctggtaattc tggtgggcat
    58681 gtcgtttttc gtccagaaaa agctccatca gagattctta tcatggatac tccaaatgtg
    58741 gcgaatgcaa agcgagtgtg gcgttggaat ttaaatggct taggttattc agacaacgga
    58801 gtaaatggcc cgttcggtat tgctatcaca tcaaaaggag aaattgttgc tgacttcatc
    58861 aaagtaggaa caataaatgc agaagtattc gaaacttcct ttaatgctta tggtgatgtg
    58921 ttaaaacttg taaaaggcac gcttcaaatt tggaatgaaa acaaaaaaat catggagcta
    58981 accaaaaaag gtatggaatt ctggaataga aaagaatcca ttggaacgat cggaacaaca
    59041 gattcagcag gaaatccatt tccaaatgct gtaacaccta cacctcttga ggaaaattct
    59101 ttggtaatac gtacaaatgg cgatggaaaa tacattttaa tttctccaac tgcagaaaaa
    59161 ggatttgtgt tattaggaaa cggaaaagca tattatttcg gtgatttaga tattcaggga
    59221 aaacttactg ttagaggtaa agaagtgata ccaggacaaa atggcggtcc atctggcgga
    59281 ggagaaacac ctggtgggta tcctgatgaa ttaaaaacag atgcagaaaa gagagcttgg
    59341 agaatatacg atattttgtg taataacgga tttacgaaac aatctgcatg tggaatattg
    59401 gggaacattc aacaagaaac aggaggaact tttgatcctg atactgttca aataggtgga
    59461 ccagcatacg gattagttca gtgggatggt tcttcatatc ctcttgttgg tccagcaaca
    59521 tgggatggaa aagtttatgt tcaaaactta tttaatgctg caggtattaa agaaccaata
    59581 acgagcttag atgcacaagt tcgattgctt atttggacat ttacaaatgg acaatggatg
    59641 ggcgtagtac aacctacgac ggttgatgga tttaaggcct gtactgatcc aagacaagca
    59701 gcatatgctt ttgaacgaaa ctatgaacgt ccggcagcga cacatcctga acgtcaagat
    59761 tatgcagtta actggtataa caaatttaaa gatttaaaac caggaggagc tactggagaa
    59821 gcaggactaa aacatttaga gtctttgatt ggacaacgaa ttggcaatgg tcagtgttat
    59881 ggcttgtctg cagaatattc aggatatctg ggtggttgtg gcatgggtgc tggaacaaaa
    59941 tatggtttaa ctcatgtgat aggaaatact tctgcagcat ctgatattgg tattgcctat
    60001 gattggtctg ctgttggttg gaaagtgatt caaaacccta gatatgatca attagtagtt
    60061 ggtgcaatta ttaattgggc aagaggtgga caagtgggtt catggtttgc agacggaact
    60121 tatggacata ctggcgttat ccgaggctta gctaatggtc gtatgcaaac ttatgaacaa
    60181 aacacagagt taggtatgat ttgtggaaag ttagatcgcc agtattatag tgcaagtgca
    60241 atttcttcca ttgtcatacc accaaaatag ggaggtgatt taatggcaaa gtggaatgtc
    60301 atattaagta caacagaacc ctataattac gtggggatga ttcaagttcg acaaggcaat
    60361 aaaaacactg aagttatgga agctacaatt gtcgaaaatg gtcttccata tgatttatca
    60421 gaatgtaagg tatattttga atcagttgtt ggcggaaaat atccagtcca attagaaaca
    60481 agaattgtgg atgctaaaaa agggaaaatc aa
//
LOCUS       DACEOE010000002        53923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEOE010000002 DACEOE010000000
VERSION     DACEOE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.002"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(78..701)
                     /locus_tag="IW774_000078"
     CDS             complement(78..701)
                     /locus_tag="IW774_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1152459.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNKLFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(765..1433)
                     /locus_tag="IW774_000079"
     CDS             complement(765..1433)
                     /locus_tag="IW774_000079"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1152460.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(1445..1885)
                     /locus_tag="IW774_000080"
     CDS             complement(1445..1885)
                     /locus_tag="IW774_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152461.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(1944..2615)
                     /locus_tag="IW774_000081"
     CDS             complement(1944..2615)
                     /locus_tag="IW774_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1152462.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(2800..3198)
                     /locus_tag="IW774_000082"
     CDS             complement(2800..3198)
                     /locus_tag="IW774_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1152463.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(3195..3968)
                     /locus_tag="IW774_000083"
     CDS             complement(3195..3968)
                     /locus_tag="IW774_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1152464.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(3965..4648)
                     /locus_tag="IW774_000084"
     CDS             complement(3965..4648)
                     /locus_tag="IW774_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1152465.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(4815..5702)
                     /locus_tag="IW774_000085"
     CDS             complement(4815..5702)
                     /locus_tag="IW774_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1152466.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(5699..6568)
                     /locus_tag="IW774_000086"
     CDS             complement(5699..6568)
                     /locus_tag="IW774_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1152467.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(6705..7991)
                     /locus_tag="IW774_000087"
     CDS             complement(6705..7991)
                     /locus_tag="IW774_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1152468.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            8264..8842
                     /locus_tag="IW774_000088"
     CDS             8264..8842
                     /locus_tag="IW774_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1152469.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            8842..9771
                     /locus_tag="IW774_000089"
     CDS             8842..9771
                     /locus_tag="IW774_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1152470.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            9768..11402
                     /locus_tag="IW774_000090"
     CDS             9768..11402
                     /locus_tag="IW774_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1152471.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSINQLLSNAQHHYEQTIQD
                     EKKKRSLEIQTMQAQIQPHFIYNTLTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKK
                     EEVIPVKEEIKSVQSYIDLLSMRYGERISAKIMIPDELQSLSMPNMILQPIIENAYLH
                     AFHNKTQGFITVYGKTKNQLLLFEVIDNGDGFDTENTKQKNDHFSGIGISHVDERIRL
                     MYGDAYGLTIQSVIGTGTTVTICLPLLPKNQSEYQK"
     gene            complement(11465..12301)
                     /gene="pheA"
                     /locus_tag="IW774_000091"
     CDS             complement(11465..12301)
                     /gene="pheA"
                     /locus_tag="IW774_000091"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1152472.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(12298..12807)
                     /locus_tag="IW774_000092"
     CDS             complement(12298..12807)
                     /locus_tag="IW774_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1152473.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(12800..14095)
                     /gene="aroA"
                     /locus_tag="IW774_000093"
     CDS             complement(12800..14095)
                     /gene="aroA"
                     /locus_tag="IW774_000093"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1152474.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(14108..15199)
                     /locus_tag="IW774_000094"
     CDS             complement(14108..15199)
                     /locus_tag="IW774_000094"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1152475.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(15216..16382)
                     /gene="aroC"
                     /locus_tag="IW774_000095"
     CDS             complement(15216..16382)
                     /gene="aroC"
                     /locus_tag="IW774_000095"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1152476.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(16384..17448)
                     /gene="aroB"
                     /locus_tag="IW774_000096"
     CDS             complement(16384..17448)
                     /gene="aroB"
                     /locus_tag="IW774_000096"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1152477.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(17481..18503)
                     /gene="aroF"
                     /locus_tag="IW774_000097"
     CDS             complement(17481..18503)
                     /gene="aroF"
                     /locus_tag="IW774_000097"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1152478.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(19162..20136)
                     /locus_tag="IW774_000098"
     CDS             complement(19162..20136)
                     /locus_tag="IW774_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1152479.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(20117..22000)
                     /locus_tag="IW774_000099"
     CDS             complement(20117..22000)
                     /locus_tag="IW774_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1152480.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(22018..24243)
                     /locus_tag="IW774_000100"
     CDS             complement(22018..24243)
                     /locus_tag="IW774_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1152481.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            24358..25392
                     /locus_tag="IW774_000101"
     CDS             24358..25392
                     /locus_tag="IW774_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1152482.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(25459..26472)
                     /locus_tag="IW774_000102"
     CDS             complement(25459..26472)
                     /locus_tag="IW774_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1152483.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLIWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(26550..26738)
                     /locus_tag="IW774_000103"
     CDS             complement(26550..26738)
                     /locus_tag="IW774_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1152484.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(27178..27306)
                     /locus_tag="IW774_000104"
     CDS             complement(27178..27306)
                     /locus_tag="IW774_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1152485.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(27309..28682)
                     /gene="rlmD"
                     /locus_tag="IW774_000105"
     CDS             complement(27309..28682)
                     /gene="rlmD"
                     /locus_tag="IW774_000105"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1152486.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(28793..29848)
                     /locus_tag="IW774_000106"
     CDS             complement(28793..29848)
                     /locus_tag="IW774_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1152487.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(29858..31288)
                     /gene="gatB"
                     /locus_tag="IW774_000107"
     CDS             complement(29858..31288)
                     /gene="gatB"
                     /locus_tag="IW774_000107"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1152488.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(31285..32757)
                     /gene="gatA"
                     /locus_tag="IW774_000108"
     CDS             complement(31285..32757)
                     /gene="gatA"
                     /locus_tag="IW774_000108"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1152489.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGNPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(32757..33062)
                     /gene="gatC"
                     /locus_tag="IW774_000109"
     CDS             complement(32757..33062)
                     /gene="gatC"
                     /locus_tag="IW774_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1152490.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(33168..35204)
                     /gene="ligA"
                     /locus_tag="IW774_000110"
     CDS             complement(33168..35204)
                     /gene="ligA"
                     /locus_tag="IW774_000110"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1152491.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(35221..37455)
                     /gene="pcrA"
                     /locus_tag="IW774_000111"
     CDS             complement(35221..37455)
                     /gene="pcrA"
                     /locus_tag="IW774_000111"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1152492.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            37610..39169
                     /locus_tag="IW774_000112"
     CDS             37610..39169
                     /locus_tag="IW774_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1152493.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(39280..41211)
                     /locus_tag="IW774_000113"
     CDS             complement(39280..41211)
                     /locus_tag="IW774_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1152494.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(41229..42143)
                     /gene="pfkB"
                     /locus_tag="IW774_000114"
     CDS             complement(41229..42143)
                     /gene="pfkB"
                     /locus_tag="IW774_000114"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1152495.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(42140..42892)
                     /locus_tag="IW774_000115"
     CDS             complement(42140..42892)
                     /locus_tag="IW774_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1152496.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(43051..43260)
                     /locus_tag="IW774_000116"
     CDS             complement(43051..43260)
                     /locus_tag="IW774_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152497.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(43271..44599)
                     /locus_tag="IW774_000117"
     CDS             complement(43271..44599)
                     /locus_tag="IW774_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152498.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(44964..45551)
                     /locus_tag="IW774_000118"
     CDS             complement(44964..45551)
                     /locus_tag="IW774_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1152499.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(45634..46884)
                     /gene="clpX"
                     /locus_tag="IW774_000119"
     CDS             complement(45634..46884)
                     /gene="clpX"
                     /locus_tag="IW774_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1152500.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(47131..48420)
                     /locus_tag="IW774_000120"
     CDS             complement(47131..48420)
                     /locus_tag="IW774_000120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1152501.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(48574..49506)
                     /locus_tag="IW774_000121"
     CDS             complement(48574..49506)
                     /locus_tag="IW774_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152502.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(49663..50322)
                     /locus_tag="IW774_000122"
     CDS             complement(49663..50322)
                     /locus_tag="IW774_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1152503.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(50325..51350)
                     /gene="sppA"
                     /locus_tag="IW774_000123"
     CDS             complement(50325..51350)
                     /gene="sppA"
                     /locus_tag="IW774_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1152504.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEKGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(51363..51653)
                     /locus_tag="IW774_000124"
     CDS             complement(51363..51653)
                     /locus_tag="IW774_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152505.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            52062..53867
                     /gene="glmS"
                     /locus_tag="IW774_000125"
     CDS             52062..53867
                     /gene="glmS"
                     /locus_tag="IW774_000125"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1152506.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINCLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT        14995 a        11604 c         9592 g        17732 t
ORIGIN      
        1 ctttttgtct tctgatttcc atataatttt atcactcctt tatgaaaatt ataacatatc
       61 tcctttgaaa ggggatacta ctttttaatg atgctctgca aggtaaggat ttcattcaga
      121 tttcctacca cataatctgc cgggatctca cccagaaaat gatcgatgtc gtaaagacaa
      181 gtagcgactc ctgcattttt ccctgcttcg atatctaaac gacgatcgcc gatcatcatc
      241 gtgtctgttc tttctaaatg aaaagtatcg atcaaataat ttaaagatgc tggatcaggt
      301 ttacgaggaa agtcctgatc gatccctact acttcctcaa tcagatgtgc caatcgatgt
      361 tcttttaaca ggccccatgt tgattctgtc aaacggtggg tcaggatgaa atgacgaccg
      421 ccgttgtctt ttagcatttc cagtacttgt ttcgtttctg gaaatggacg ggaaacttcc
      481 gtactttctt tttcatatcg gtgaaataat ttattaaact ctctggcatt caagtttgat
      541 tcattgatca aataactagt agaatacttt ttcattttga agtagatttc ttttttgtcc
      601 atactgatac caaaatcttt tagtgcttgc catgcaccat ctaccattgc gggatacgta
      661 tcaaacaatg taccatcaaa gtcccatata taatttttca tgttcctttt gttccattcc
      721 tttgcttctt ttttctatct ctcactttta gataaaggtg ttctttaaag tatttttttg
      781 tccagaattg tcaaatcatc tgctaatgta tactggattg gttgtgcatt tgggatatcg
      841 atcgtatcca tttgatcttc aggaacatct tctaaatatt ttgtcagtgc tcggatgcta
      901 ttaccatgac ccacaactag taaatcttgc cgatctttta gttttggtgc tagatgatct
      961 tgccataagg gaatgatccg attcactgtc atttccagac tttctccgta tgggatcaaa
     1021 tgcggatcca agtgatcata tctgcggtca aatctatttt ctgcggttaa cggcggaact
     1081 tccgtatatc ctctgcgcca tgtttttact tgatcttttc cgtatatttc tttcatctta
     1141 tctttgtttt tacccacaag atccccataa tgccgttcat ttagacgcca tgtttttatt
     1201 ataggaagcc ataactgacc catttcttct agtgcgactt gtccggtaag aatcgcacgt
     1261 ttcaaaacag aggtataaac ggcatcgaat tgaagtccgg cttcaagcat tttttgaccg
     1321 gcaactgctg cttgttcttt tcctttttct gttaaggcga catctagcca tcctgtccag
     1381 taattttcaa aatttgcttc gctttcgcca tgacgtaata atactaattt cattcttttc
     1441 tcccttaaac attcgtttcg aattttatac cacatgtttt gcatgtcaca cgtattcgtc
     1501 cttttccttt aggtgcacgc tgcttttgat gacaattcgg gcactcaaaa aaagtataac
     1561 tttttcgatc cattttgact tgctttttgt tttttatctg atgatatttt tctttgactc
     1621 tatcaagata tttcaaataa cgttgattct catttagtct ggggtagatt ttttttgata
     1681 ggaaacgaaa tatcatccat ccgaaaaaga cgatgaacaa caattgtccg attctatagg
     1741 gcaaccaacg acttagcaaa ctaaagacaa gcgcagcgat caatagaccg cgattcaact
     1801 gatcaaattt cgcatatctt ccctgcataa gtttgtgata gttctgctga aatcgtatta
     1861 gcctttctaa ccatgtttgc atcatattat ctcctccatc atctaatatt tattatgaca
     1921 aaaaaatcca aaaagaaaaa gccttaattg gccttttcca ttgcaacttg gtgaaggaca
     1981 ttccgatttc tggtaaagtc aatcacaatc actttctttt tatcttctct gttgactact
     2041 cgaaatgtgt ccgtcaatgg aagaaacagc ttcttagatg gtagctgttg catacgtata
     2101 aaacgatgga tcatcgtaat caattgagga aattgaatag acgtgtgaag cagacctgga
     2161 tattttttca acagctgtgt cgttttgatc agattccgtt ttttactgat cacttttaaa
     2221 aagcttttta gaatcttttg ctgacgtcta aaaatagaaa aagaattttc gtcatctatt
     2281 tgataacgca tttgtttttg cagttctgct gcagtcagat ggatcttccc atcgtttgta
     2341 tcgatcatta aaccttctgg aaacagttca accactgctc gatcccaaga ttcaagagaa
     2401 agctcgatat aatcttttac tggtaattcc aattctacct taaaaaagtg caaaacagcc
     2461 gacagccctt ctgcttgata gatttctcct aatgttagcc cctctagatg gaactctggt
     2521 gaaaaatgca gcaatgtttt attttcctgc aattgaacat atagaaatgt agatcgctca
     2581 atcagagtat cttttaaaac aactaaaaaa ttcatttttc tccccctttt ttcatcattt
     2641 ttaaaatagc atattttgaa aagaaagtct gatgcttaga gtagaatttc ataaaaattt
     2701 actatttcat aagtattcac gagaaaaata caaacaaaaa agcggaaata acgctcatcc
     2761 gccttcttgt ttgtgtttat ttcctcttca aacgcttgtc tatgattcag acttttctct
     2821 atcgttattt tcatctaaaa gttcttgcat tccgataatt tctcgatatt gatgaatctt
     2881 agtgatacca atgactttgt ctacatcaat actaaaacaa ataccattac ctggaatatc
     2941 gatgttgatg ccttgaccaa tcgcatccat tactttattc gtcaaactat cgggtactaa
     3001 tgtcaacaca atatcttttt ctggcgaaat gtccaaacct aaaaatttcc ctgaatcatg
     3061 tacaccagaa cctcttccat gtaagatcgt tccgcctgct gcacctgctt cttttgaaaa
     3121 gtcgataaca tcttcgccca ttcctccgtc aacgatcgtc acaatcatgt gaagattcaa
     3181 tgctacaaga tctttcattt gctttccccc aatttttgca atctttcttt ttcgattctg
     3241 tctttttcat cttttcgttt ataaatatat cctagtacta aagtggataa tattggtgtc
     3301 aatgcaatca ttgcaaccac accaaacccg tcaatgatcg gatctcttcc ttcaatttgt
     3361 gcagcgattg ccacgctcat tgataaaata aatgtcgaac acatcgggcc cgttgctact
     3421 ccaccgttat caaaagccat tgctaaaaag atcttgtcca cttttcgtcc taaaataaat
     3481 gctagcccgt atcctgggat caagaaataa taaagagaga atccgacgag caaacgccac
     3541 atagacaatc ctactgctgc gccgatccca attgaaatta ccatcagcat gattttcgat
     3601 cggattctcc cctcgctcaa ttcttcaacc tgtttgatca ttacatgaat cgccggctca
     3661 gcaaacccta ctaagaaccc cattaaaaag ccaaggggaa ccagtatata attgtaaggc
     3721 aatcccgcaa tagcactgcc taaatgctgt cctaccggta cataagccat attgactccg
     3781 tgcaggaaaa ggatcaaccc aatagtcgtt atcaagaaac ctttcataat gtcaaagaaa
     3841 cgctttttgc gcatacgaaa actaatgata ttcattaaaa caaataggat tacgataggt
     3901 gccactgcca aaatgacttc catggtaacg atttcgattc catctatcac ttgctgccag
     3961 aaactcattg gaagatcacc cccaaaatca tcacggcaag aatcggccct aacgaagcaa
     4021 taccgaccat tccaaaactg tcattttcat ttttcttttg tttgatcata ctagtcatcc
     4081 ctccggcaag ggccaagata aatggaacag ttactggacc agtagtcact ccccccgaat
     4141 caaaagcaat cggcataaat tcggaactgg tgaaaaatga ggcgatcaaa acgcctatgt
     4201 agcctatagc catcaattga taataggata gcttgaatac gacccgaagc agcgcaaagg
     4261 ctagaaatac tccagtgccg acgctgacga tcccaatcag caacactctg ccaatctctc
     4321 cgtcagatat atcatatact tgttgtccca aaacttgcac ggaaggttca gcaatcgtga
     4381 tcacgatccc gatagcaaat cccaaactga ccaaaacggt caaacttttc tttttgatca
     4441 tatatttccc tactagatct ccgacttcca tcatagaata atctgctcca aacaaaaaga
     4501 gcgtcattcc gatcatcatg attgccgcac caaccagaaa tgaagcgagg tcttctcctt
     4561 ctaacggtgc aaaaacaaac gttaaaatga caatcaggac agtcatcggc aatatagcag
     4621 caatcacttc tttaaaattt tttagcatgt atcctcctcc tttactcttt aagtttacct
     4681 taaaatgaaa gagttttcga gcacaaactt aaaacgttat catttaattt ttctcaacat
     4741 aaaataacac acgtcccaaa agtatctttt tacttggaaa aatgactttg aaggaacgcg
     4801 cttattgtgt ttttttattt tacagagccg atcattcctt gaacgaattg cttttgcaaa
     4861 acaaaaaata cgatggcagt tggtaaagta gcgatgacta atccagccat gatcattcca
     4921 tagtctggtg tataggaagc acccatagca gataaaacaa gcggaaccgt ccgtttatca
     4981 ggagattgca aagctactaa gggccataaa taattgttcc agctactcat gaatgtgatg
     5041 attgcagccg cggcataggt atttttagcc gtcggcatat acactctaaa aaaaatactt
     5101 agttctttta gaccgtctaa tcgagcggct tctactagat cttttggaaa agatttaaca
     5161 ttttgtcgaa agaagaagat caaaaaagca gtgctaacag atggcaggat gacaaccaag
     5221 aaactgttga ttcccaaaac cgttccgttc aattgtgaaa acattcgata taaagggatc
     5281 atcaatgccg caaaaggaac catcatcgat agcagtagaa tattgaaaat taggtctttt
     5341 cgtttacttt gataaatctc aaatccatat cctgccattg acgaaatcag caatgcaaac
     5401 acagtggtca ccaaagcgat catcgctgaa ttccaaaggg aacgagtaaa attcagctca
     5461 ttggaaaaca aattttgaaa attgatgatc aagttactgc caatctttat ttttcccgat
     5521 gtgatatcca cgggtgtatt cgtcattccc aaaaccatcc agacaaaagg gaaaatggaa
     5581 ataaatgata ggatgcttaa tgctgtatac tttaatactg acccaattgt cgaacgaagt
     5641 tctaatgctt gttttttgac gttggggcgt tcgattgttt ctttttctac catatttttc
     5701 atcgactttc tcctcctact ttcgtttgga ttatcgacaa tatcacaata aaggaaacaa
     5761 taacaaagga aactgctgcc gcatatccaa aattaggcgt gaatttataa ctcaaattat
     5821 agatgtattg agatagggtg gtcgttgcat ttgctggtcc cccgccagta atattttgta
     5881 cttcatcaaa aagctgcaat gtcccgatcg tcgaagtaat ggaagtaaac aaaatgattg
     5941 gttttaaatt tgggatcgtc actttgataa actgctgcca tttagaagca ccgtcaatag
     6001 atgctgcttc ataaatctcc gaatcaatat tttgcatgcc tgaaaggaaa aaaatcatat
     6061 tgtatccagt ccaacgccaa gtgatcgaaa gaatgatcag aactctcgcc caaattggat
     6121 gagtcaacca gccaatcggt gtgtcaatca taccaatgcc caacaacaag ttgtttacaa
     6181 ggccagattg agaaaataga cttttcatga tcagagaata actgaccatc gaagtgatac
     6241 atggaagaaa gatagcggtt cgaaacaacc ctttatattt caacttttta tcatttaaaa
     6301 ggttggaaat aaaaagtgct agaaacaaca tgatcggtac ttgtacaagt aaatacagga
     6361 atgtattaaa aaaagctttt ttaaaagttg catctgtcaa catccgctga taattaccta
     6421 acccgttaaa tgtcatattg acagggggac cactttgaaa agacgtgatc aaagccaaaa
     6481 ccatcggaat aaaaaccatg attgatatca aaatgactgg caagattaaa aaagcatgcc
     6541 cgtttttgaa taaatctttc tgcttcattc ttttccctcc aattctatca ttgattatct
     6601 acatgaaaag acaagaaaga caatatctgt aaggcagacg tgatcttttt tgtcttttcg
     6661 tttgtctgat gaaaaagcga agaaattcac ttttttacca ccgatcaatt agccaattgt
     6721 gcttctactt gtttttgtgt atctgctagt accttgtctg tctcttctcc attgatgatt
     6781 cgatgaagag cttcagctac gactgcttca attgcatagg tctcatgtcc gtaattcact
     6841 tctggtattt cagatgtcca tgttgaaaaa tcgtcaaaga tcttttgacc actgtaaaat
     6901 tcagaagctt cttgataagc ttcttgttca ttcgcgctaa gcatcgtaga tactagtccg
     6961 atttctttcg caagagtgcc catcagttct tcattcgaag caaacgttgc ttctaggaaa
     7021 tctttcgctg aatcttctcc agctactcct ttgatcacat accagccagc gccgccaagg
     7081 ttagaagctt gcgctttttg cagattttct ggaagtgcag gtattggtgc aatcttccat
     7141 tttccagatt ggttttctgc tccttggatc gttgaagaat accaagcgcc agttgggcta
     7201 gaagctactg ctcctgattg tatcgcagtt actccagcat tccagtcaga ggtttgttcg
     7261 actagatctt ctttcaaaag agtggcaaac aattcaagac catactttaa agatgcattg
     7321 tcttggatcg tgactgtctc cccatcttca gatgtatacc attctcctgc ctgttgcata
     7381 atcatcctta ctcgaccaag gtcagaagga ttgacttcag cgattttctt tcccgtcttt
     7441 tctttgacat ctcgcgctac ttgaatatac tcgtcccact tcagattctc catatcctct
     7501 tctgtataac ctgcttctgc catcagatcc gtacgataga agtttgctgt tacgcccgaa
     7561 tcgaaaggaa caccgtatat tttatctcct actttgttta ccgcaaattt atacggtgca
     7621 aaatgatctt cctttacaat agcagacaaa ttagcgaatg catctggata agaagtcaaa
     7681 tagccttgta tcctataatc ttcaatcaag acgatattgg gcaatccttc tgtattcccg
     7741 ctcgctaaag cggtgttcag tttctgaacg atatcatcct gagacatcgt aacaacttct
     7801 atctctgttt cttcgttctc ataaactttt tttgcttcat tcactgcttt gatattgaat
     7861 gtctcatccc acgcccagac agtgactttt tcttctttgt ctgctgtact actagcactt
     7921 ccgcatcctg ttaagaaacc tgatgctgcc gctaaacaaa cgactccagc aattaacttt
     7981 ctgatgttca tttttatccg ctccttttcc atccattagt tgtttacgtt tgctacattc
     8041 atcataccaa aatttgaaaa cggtttcttt attattcaga tacttcaaaa atttctccta
     8101 aaataataaa aaaatacaaa tcaactatga caaaaggaat aatcttagca tctagaccaa
     8161 agatcgaaat ggtgaggttt ctttaaaata aaaatatcct tgcattttct cctagaaata
     8221 agtattttta aattacaaga ctattcttaa ggcgggtaaa aaaatgccaa tacgcgtaat
     8281 tcttatcgat gatgaacaga cgattttaga tggaatgaaa cagttgatca attggtctga
     8341 gcacgggttt caattaatag ggactttttc cagtccgctg aaagcgcttg atttttcttt
     8401 ggtacatgca atcgatatcg tggtcacaga tgtaatgatg cctgaattaa atggaatcga
     8461 attatcgcgg ctattaaaac agcaacaacc tgaaacacag atccttatcc tctctagcta
     8521 tgatgaattt gatatggtta aagattcttt taaagaaggc gtcgctgatt atcttttaaa
     8581 gcctcgattg agtcctgatt tttttctaaa ttccttaact gccgtttcac aaaaaatcaa
     8641 aaaaaagccc aacaaaagcc caatcaccaa tcgttatgag cagatctgcg agaatctttc
     8701 acagcagatt gctggacaaa ctcgtccatt gcttgaccct cttctttttg aacacacctt
     8761 gtttttctgt tatataccga aaaacggcca ttcagttcaa aacaaggctc taaacaaaaa
     8821 cagtcgatca cccattcatt gatggatagc gacgattcgt ttactattta tcctgtttct
     8881 accttttcgg aagaatccgg ctatgtaatc aatactgatt ccaaagatac acttaggaag
     8941 tttgttcaaa agtttcgtca agaagttttt ccaggtgatt tttttgttgt ttctgattct
     9001 tttgcaatcc aagatatcaa tgaaatattc caagagatca gaaattgcac caaaggacaa
     9061 cgtttctatc ataagggaaa gaaccttgct tatcagcatg aacttcttcc gttattaacc
     9121 aaccacgagc agcaagcaag tagttattta acaaaaatcg ttcataaaga ctacgcttta
     9181 gctgtcaaag aacttgagaa tttcttcacg acgatattgg aacagcctat tgatccagct
     9241 tatttgaaac atgaggcaat caatcgtttc tatgcattac tcaacgcttt ggctgacgag
     9301 catgttggga atgaagaact ttctttgctc aagatcgccc ttccttcttt attagccgat
     9361 gctgagtatt tcgaagattt ttctattctt gtcaaagaaa cacttgagaa actcacccac
     9421 ttttctgcaa atgcaaaaaa agacaatttc gatatcttga ctttgatcta cgagttcgtc
     9481 caggaaaatt atgagaaaga aatcaattta caacttttat ccgaaaaata tcatttttca
     9541 tatagctatc tctcagccat ctttactgaa aaatttggta tcaacttttc aaaatatcta
     9601 aaaaaagtcc gtatcagtaa agccaagctc tttttgacgg aaacacggct tactttgacg
     9661 gaaatctgtg agaaagtcgg ctataccgaa ctgggttatt tctccagagt ttttaaagaa
     9721 gagacaggaa tgacaccttc tcaataccgt aaaggaactt ttgtaaaatg agaaaaaaaa
     9781 tcaaagagca tgaactcttt gccaaacttc tgttcatttt gttcatcggt ctttttctgc
     9841 aagccatcgt catttctttg tttatttatc atcgatcaag ggatgcctat atccagctgt
     9901 tcaatcaatc gaatgatgtg gtactaaaaa agattcaatc tgaatttgaa agtttgaatg
     9961 atacaatcga gaatacatta gccgcttttg attctaatcc agctgtcaaa agctattttt
    10021 ccaatgatcc agcccaacat atggaacagc ttcagcaact aagaacaatc caaaaaatga
    10081 atgactctct ttcaaaaatc catccaatga tcgattacga tgtattgatc tttggagaaa
    10141 atggaagaac ttttgtcgga aacgacatgc tcactgccgt atctgccgat tctttttttc
    10201 aatctgcgat tgctcagaga gtgaatgaac gtgctgctga tacacaaatg ctttttgcac
    10261 accacggatt gactcttcgc gataagaaag cacccagcgt cttctttgtc aaaaaactga
    10321 aaaatacatt gaatcacgtt tacggatatg ctgttttatc gatctcatcc aagcaactag
    10381 cgaatctttt tcaatctgta gtaaatcctg agatatcgaa gatctccttc atgaacgaag
    10441 aacagacgat catcgcatca aatgaagaaa atacaattgg gagccgatca atgattttca
    10501 aaaaactaac agtaggtgaa acaacgattt caaatggaca gcagttgacc cgtcttcctc
    10561 tgtaccgcca ggattgtaca ttactcagtc aagtcgatct tcattcttta tctaaccaaa
    10621 tgggcgtgat ttttccgatt attttgtata atatcgtgac acttgctttt gctggctcat
    10681 tggtatttat gtatttggat cgacacacaa aatcgatcaa tcaattgcta agtaatgctc
    10741 agcatcatta tgaacagacg atccaagacg aaaagaagaa acgttcgctt gaaatccaaa
    10801 ctatgcaagc acagatacag ccccatttta tttataatac gctgactagc ttgaaatttt
    10861 tgatttggca gaaaaaaaca gatgaagcag ttcaaggaat cgacagtttc attactttac
    10921 taagaagtac gattgggaaa aaagaagaag tgattccagt caaggaagaa atcaaaagcg
    10981 tacaaagtta catcgatctt ttgagtatgc gatatggcga aagaatctcg gctaaaataa
    11041 tgattcctga tgaactgcag tcattatcta tgccgaatat gatccttcaa ccaattatcg
    11101 aaaacgccta tcttcatgct tttcacaata aaacgcaagg attcatcacg gtgtacggca
    11161 aaacaaaaaa tcagcttctt ttattcgaag tgattgataa cggcgatggc ttcgatactg
    11221 aaaataccaa acaaaaaaat gatcattttt caggaatcgg catcagccat gtagatgagc
    11281 ggatacgcct aatgtatggc gatgcatatg gcttaacgat ccaatcagtg attgggacag
    11341 gaacgactgt aacgatttgt ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat
    11401 aaagaatagc caagcgctga tttttttatt ttaagttaaa taaatcaatc agtattcttg
    11461 actttcatct acgcgtctct tctactgtca gcactggata acagcccaat atttgacttt
    11521 ctcctccgag catcttgatt tcttggagcg cattctctac taatgtcatt ggacggtcca
    11581 acaacaaatc gatcacgaaa aaatattctc ccaagctagt tttcaatggt cgggattcaa
    11641 tcttacttaa gttgatttcc cgccagccaa aagctgccag cattttatga agcatccctg
    11701 gccgattagc aggtagtgtc aagatgacac tcatttttac tggagctgac tgctggctgg
    11761 tcatctttcg gtcaccgacg atccaaaaac gtgtctggtt caactcatta tcttggatat
    11821 tctcagctaa gatctccaat ccaacatgct gtgctgtttc taatgaagca atcgctgctg
    11881 cgtcttcttt cggatgttct gctacataca ttgctgcagc agttgttgat tctgttgcta
    11941 ctaaaggaac attaggataa tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg
    12001 gatgtgaaag gatttttgta atctttgttg ctggattgcc taaaagctgc tgcttgatag
    12061 gaagaacgat ttcactttta acttcgactt ctggatgttt ggaaagaaga tcgattgtgt
    12121 gatgaacagc tccttccaat gaattctcta caggaacgac agccagatcg acttgtttgc
    12181 gaaacaaagc gcgcaaacaa acagggatgg aagcatacgc agtcaattgt tctgtaggaa
    12241 atagctgaca agccgcctga taagtgaatg aactttctgg ccctagatag ctgacgttca
    12301 tatgcttttg atcctttcta taatggtatc gatgatcttt tctggtgttt gattggtcgt
    12361 gttgattcga tgtgtcgcac tttcttcata ccaagaaagt cgttgtgcca ataatgcttt
    12421 tatttcttct cttgtttttc caagagctaa aggtcgaata gacgtttcat cttgttggat
    12481 acgatcaact agacagtccg cttctgcctc taagtaaaga acaatcggct gtgtttttaa
    12541 aaaacgccga ttttctgcac tttggacaat ccctccacca gtcgcgataa ttttgtcttc
    12601 atttgacagc tttcttaaaa tatctgactc cacttttcga aagctttttt caccgtaata
    12661 gtgaaaatat tcggcaatcg acaattggat atgttcctca atctttgtat ctaaatctac
    12721 atactctttt tgtaaacatt gagctaaccc tttaccgatt gttgtttttc cagcgcccat
    12781 gaaaccaatc agcaaaatgc tagccatgag cttctcctcc tgacacaagt tctgctaaat
    12841 cgtcaaaaaa tgccggataa gagattgaaa cagcttcagc tccttctagt tcgcaaggtc
    12901 catcagctat caaagcagct acttgcagca tcataccgat tcgatgatcg cctctgctgg
    12961 acacttttgc gccatgcaga ggtgtcggcc catggatgat cagaccgtct gctgtcgctt
    13021 ttatctttgc tcccattttc tgtaattctt ctgccaccgc atcaattctg ttggtttctt
    13081 ttacttttaa ttcttctgcg tctttgatga tcgtgattcc ttctgcttga gtagccacta
    13141 aagctagaat gggcaattca tcaatcagac gcggaatgat ttctccacca atcgttgctt
    13201 tcttcaaatg actcgtcttg acacttaaat cagccgattg gttctgttca ttgtgattcg
    13261 tctgggtaat cgctgcgccc atctcctcca acacatctaa gataccagtt cttgttggat
    13321 tgaccccgac attttttaac aacagttggc tttccggaac aagaagtcct gctgctagaa
    13381 agaaagcagc tgatgaaata tcacctggaa cagttatctg ttgaccagtt aatttttgcg
    13441 gtccggtcac tatgatcgtt ttatcttcca ctgtgatcct tccgccaaat tgacgaatca
    13501 tttcttccgt atggttacgg gaacgttctt tttcaacgat tacggatgtg ccttctgctt
    13561 gtaatgccgc aaacagcaaa gcagatttga cttgcgcact ggctacaggc atattgtatc
    13621 ggataggtga taaagagcgg gttcctgtca cagtgattgg tagatactgg tcattttctt
    13681 ctccttgcaa gtcagcgccc atctcgcgta aaggttccat cactcgcccc attggacgtt
    13741 tagacaacga agcatccccg aacagagtag tcgtaaaagg ctgtccagcc aaaataccca
    13801 tcaataaacg tatggtcgta ccggagtttc ccatgtcaat tgattcggtt ggttggacaa
    13861 atgagtggct accgcgacca tgaatcttga tcacctcttc ctcttcttcg atctcgacac
    13921 ctaattgttg gaaggcatgt aaagtactca gacaatcttc tgcgcgcaaa aaattttgta
    13981 tagtcgtcgt tccttcagca attgctccaa acatgatgct gcgatgggaa attgatttat
    14041 ctgctggtat cctaacagtc cctcttaatc catgtatttg ttgtaataat tgcatttatc
    14101 cagcctccta tttgatccag caatgataac tggtattaga cataatgatt ctttttgctt
    14161 gtaacaaatc ctgttggttt ttaaaactga tttgcagtat tcctaaaata tcttccctgg
    14221 tctcttggat cttcaaatta atcaaagaaa ctcccgcttt tccgatcagt ccagttactt
    14281 ctgcaataac gccaggttca tctggcacat cgataaataa atcaaaaaag gcaggaatcg
    14341 cgccttgctt atggacaggc aactggtctc gactttcttt tgcccgattg aaaaaagaaa
    14401 agatttccga ttggttctca gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc
    14461 aatctgccaa tcgttccaat aatatctgct tattgctcaa caaaatatct gtccacatga
    14521 caggatctga ggaagcgatc ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg
    14581 ctcgcggata ctcttgattg aattgttctg cctgattgac taagccagct gccagaatat
    14641 gtggaaaatg acttagcatt cctgttatac ggtcatgttc tcttgctgta agagttacat
    14701 attttgcttt ggttccgcaa aatagttctt gcagtttttc aacatctttt ttcgtctttt
    14761 ccaaaggtgt aaaaatgtaa taggcatttt caaatagttt agcatctgct gcctgtatac
    14821 ctgatttatg agaaccagcc attggatgtc caccgacaaa ccgaagattt ttctgctcag
    14881 cggatgtcac gacttgctgc ttcgtactgc tgacatccgt aatgatgacg tgatcagaca
    14941 agttgattct ttcaaggaca tcaagataag ctaaggttgt acggacggga acggctaata
    15001 agataacgtc tgcttgttct gcacctgttt caaaatcact tgcaacgtaa tcaacgatct
    15061 gctgttctac tgcttgctca cgggtttttg actgactatc ccaaccaact actatcagtt
    15121 ctggattggt ctgacgaata caaagtgcca tagaagcacc aatcagtcca agccccacga
    15181 taaaaacatg ttgtgccatc ttatttgccc cctttttagt aatgtttcaa gtagtcacgg
    15241 tactccttca ctgcagcttt caactcaaca agcgaatcac ttgggaattt tgacaatagc
    15301 tctgtcgcta ccacagtggc tacaacattt tccgcaacga cgctagctgc aggaactgct
    15361 gtactatccg aacgttctac acttgctttg taaggttctt ttgtatcgat atcaacactt
    15421 tgaagtggct tgtacaatgt aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg
    15481 ccattggtca ttccgccttc gaagccgcct agattattgg tacgtcggaa atagcctctt
    15541 tcttcttccc ataggatctc atccatcact tctgagcctg gtaatgaacc agaagtgaat
    15601 ccgaccccaa attctacgcc tttaaaggca ttgatactaa ccacagcttg tgcgattttg
    15661 gcatctagct tcgtgtccca ttgtacgtag cttcctaatc cagctggaac gcctccaacg
    15721 agcacttcta caattcctcc gatcgtatct ccatttttct ttgtctgatc gatcaaatct
    15781 tttattcttt gctcaactgc tggatcaaca actcgtactt ctgacgcttc agaaatttct
    15841 ttgatttctt gaatcgagat atttccaggt atttctgctt ggattccacc aagacaaatc
    15901 acgtgtcctg ctacgatgat gtcaagttct tccagaattt tcttggcaat tgcgccgatt
    15961 gctacacgca tggtcgtctc tcttgcagag gaccgttcaa gcacattccg aagatctcga
    16021 tgatggtatt tcataccgcc taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc
    16081 cgcagtgatt ggtctttttc aggcacttct gctacagaca tgacagttgt ccaatttttc
    16141 caatctttat tttctaccac taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg
    16201 ccagacgtga tccgaacacg atctttttcg atcttcatcc ttcctcctct gccgtaacca
    16261 gcttgtctgc gagcgagttc gtgattgatt tcttcttctg acagaggtaa tcctgctggt
    16321 agtccttcaa taatggctgt taattctggt ccatgggatt ctcccgcagt catataacgc
    16381 atcttacagt cctcctaagt aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc
    16441 ttgcccaatc ttttctagca agatgatatt caattggctt cctcttgctt tcttatcatg
    16501 ggttatcgcg tggaataatt catctggatg acttagatcg tacgtaatcg gcagatggaa
    16561 tttttgaagt atcctttcta aagcagctgt cgatccagtt ggagacaagc cgattttttc
    16621 tgcatgagaa gtgatggcta ccattccaat agctactcct tctccatgac tgactacccc
    16681 atatcctgct gtattttcaa ttgcatggcc aattgtatga ccaaaattta aaataagtcg
    16741 atttccttgg tcaaagacat cttcttccac tacttttcgt ttgatttcca acgtagcagt
    16801 aatgattgga acagcatgtt ccagaagatc tttttcgtct ttcagctcgt tcagctgttt
    16861 ccaaagcgtt gcatctgcta tagctgctga tttgatgatt tcagcgattc cttctcgtaa
    16921 acgacgtacc ggcaaagttt ttagaacctc aggatcaatc aatacaccag ctggctggct
    16981 aaatgttccg acaaggtttt tcgctttgtt tgtattgact gctgtcttcc cgccaatact
    17041 agaatctact tgtgcaagta gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa
    17101 cgtcgaggcc acgaatgctg ctagatcacc caccattcct ccaccaagtg caagtatccc
    17161 atcgctgcgg gtaaaatcat tatctgctaa aaaatcgtat aactcagctg cttgttctaa
    17221 agatttactg gtctcacccg ccggtacgat tgaaaggact ggttcaaaat tagcttgtct
    17281 caattggaac atgatttttt ctgcatatag tggagcaacg tttgtatcag tgatcacagc
    17341 tactttttga ggtctccaaa tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt
    17401 ttcaatatga atatcgtatt tttggttatc aacatgtaca gtaagcattc gtttcacttc
    17461 tttcttttaa aaagctggaa ttaatcgagt gtctgaatct tttccattgc ttctttcatg
    17521 atcgcaactt ctttcatcat gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct
    17581 gaccaagcat ttgcaggatc aggatggatc tctacgatca tgccatctgc tccgcttgcc
    17641 acacccgcac gagccattgg tggtacaagt tcccaaattc ctgtaccatg acttggatca
    17701 acaatcacag ggaaatgagt taattttttc atcaatggga cagcacttaa atcaaacgta
    17761 ttacgtgtcg ctgtttcgta agtccgaatc ccacgttcga taaaaatgac aggagatttg
    17821 tcttcgactg caatgtattc tgcagcatta agccattcat tgatcgtacc ggaaattcca
    17881 cgtttcaatc caacaggttt acctgtttta ccgacagcag atagcagttt aaaattttgc
    17941 atattacgtg cgccaatctg taggatatcg ctgtactcag ccaccatatc gatatgccct
    18001 tcatccatca cttcagtaat cactttcatt ccaaattcat cagccgcttg acggatgtat
    18061 ttcaatcctt cttcttctaa accttggaaa gcatatggag aagtacgtgg tttgaaggca
    18121 ccacctcgca gaatttttgc gcccccggct ttggccattc gcgcacattc tctgatttgg
    18181 tctaatcctt cgatagaaca aggaccagcc atcgtcacga aactgccgtc accaattttt
    18241 actccgtcta catccacgac tgtattgcca ggatggaatt ctcgagaagt caatttgtat
    18301 gtgtttaaaa tacgcacagc attttctact ccttcatagc gtgtaaatgc tccttcttgt
    18361 aaacttcgcg tatcaccttt tagtccaatc actactcgtt ctgttccttg atcgattgag
    18421 actttgaatc cttctttttc gatatgatcg acgacttttt ttatttgttc ttccgttgct
    18481 tcttttttca taattactat cattgttttt cccgctttct tttgtttact tatctatctt
    18541 ggctgatttt ctctactttt tcaaggtggt tttacattaa ttcctactaa atgaccgtgt
    18601 cctgttgttc tcattttcgc tccttctttc ctacttatga cccagataat caacaaaaaa
    18661 agatgtccct gtaggaaatt gcctctacag tgaacatctt caacgagcct taaaaagcgc
    18721 tgcaaaaata taaaatagcc actatagatg aatttctcta cgtggctgta agtcgtgttt
    18781 aaaaatcttt agccatcata gatacaaacc tggtttgttt gtacccacga agtcatgaat
    18841 acaaacctat ctaaaatagc taaagtaata cgtattcagt tttgtaagtt ttctagattt
    18901 cataaaaaat cgctccaaac tgatgtaaga attgtaccta gtatagacat aaaaaaatcg
    18961 actgtcaatc atttttatga aacttttttt aattttctga tagttaactt tgtttttcag
    19021 aaaaataaca attggtttta ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt
    19081 tttactctct tgttcatgac atatagaata aagcagcttt gtcttgtaaa ctccgttttt
    19141 caaataataa tctaaagatt gctactgaat aataaagccg atttcatggt cctcttcgct
    19201 gctgattcgg taaggtgact caacatccgc tccccagcta ttgatacccc cgactcctct
    19261 tacttttgca aaaatcgtaa gaaccgttcg tctttctggc ggtagttctt ctatatgtgt
    19321 tgcctgttcc agttcttctg ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg
    19381 ctgttcaaat cgcaacgaaa aatcttcttg atctttatcc gtattatcca acgtttgatt
    19441 tcttgttagt tctacccatt tcgtttccat atgcatgccg cattcttggg gaacaaggta
    19501 tggggaaacg gggagtcctg ccactttata aataccttcg acaccaccag ccatccgatc
    19561 cggatatgtt tcccctgata ggccttcata ctcaaaaaat tttgctttcg ttggcataat
    19621 gaaccttaaa cccaatgcgg gtaattccgg taatcctttt tgtccataat aatgcatatt
    19681 gacaagcatt ttaccctctc cagcgatttg gtaggaaatt gtcacttggg tttttggtac
    19741 tgtgttgatt tccagtgtaa aaatgatttc aaccgtttca gcgtattcat tacttgaata
    19801 ttgattattt actggagcaa tcggcaaatc gatcttttta ttgtccacca gtacttctat
    19861 ttctttgact tttggataca atccggctcc taaccacata ccggatttaa atccaaatcc
    19921 gctacctctg tcattatcgg tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag
    19981 ccattctttt ccgtttttat tgagcgattc cagtccgcct cttgtataag aaaaaatata
    20041 atgcgtattc cccgatttga cacctaatgt acagtcgccg aagacgattg caactttttc
    20101 cgtatccata tgacttttat ttactgtatt tttcataata atatcttacc tcctgcatgg
    20161 cgggtttttc ttgtccatct gcaaaaacga tcccattccc tgagaattca tagtcacttg
    20221 gtttatcgtc aaagtcaccg ccatagcgca tcacttctct gcctgtgatt ggatcagtca
    20281 cttggatcgc ctgatcttta aaatcccata tatagcctcc ttggtacatt tcataacgat
    20341 caagcaacgt catgtaagaa tccattccac ctaacgaatt ccccatgtca tgcatatatt
    20401 cacataagat gaacggtttt tccgctttat tttcaaggta gtcaatgatc tcttctggtg
    20461 gcgcatacat gcagcttttt acatctgaaa tcgaatcttc gaatgctgga tttggaaaat
    20521 tcccttcata atggacaagt cgatcggggt ctactttttt gaagtaatcg ttcatttgtc
    20581 gaatgttttc ttccgcataa gattcatttc caagagacca aaaaagaata cttggatggt
    20641 tttttaatgt ttcgaaattc gtttttgctc gatcaagaac agccgcctgc cactgaggta
    20701 ctgcacctgg tacgttgtaa gaaggctcaa atgcgcccat tttttgccat gagccatggg
    20761 attcgaggtt cgtttccgcc atcacatgga ttcctgcttg gtcgcataaa taataccaag
    20821 tgatctggtt ggggtaatga gatgtccgta cgccgttgat ctttgcattt tttatgatag
    20881 ccaaatcttt ttccatatct tctatagtga tggcgcgtcc ttttttcgga tgccactcat
    20941 gtcgatttac cccgttgata atcatccgtt ttccattgaa gtagatgatt ttatctttga
    21001 tttcgatttt acgaaaaccg aaggaatagt caatatactc cgttactctt ttttcttcgt
    21061 ctatcgtctc caattcaagc ttatataaat tgggctgaaa atgcccccac agtgccacat
    21121 tttcaaatcc atcgatgcga atcgtcattt gctcttttaa cggatattgc tgttgataga
    21181 tttccttccc atcaggatct agtatcgcta cattcagctg actgtttttg gctacttgct
    21241 caaaagacaa atctacttca agtttgccat tttgacattt gtcatctaag cgaggtttga
    21301 tccaaagatc ttctaaatgg ttttgtcctc gagcaactaa gaagacatct ctgaagatcc
    21361 caaaaaatcg gaaaaagtcc tgatcttcca agaatgcagc tgtacttctt ttgtaaacct
    21421 ccacagctag aatattttct tcttccaaat aaggtgttaa atcaaattca gacggagtga
    21481 acgaatcttc ggcatacccg ataaaatgac cattcaacca aataaacatt gcttgttcta
    21541 caccagcaaa atgaatcgag atttgttttc cctttagacc ttcttctaat cgaaaagtcg
    21601 tcaaatacga accaaccgga ttgtctttag cagctgaaaa gatcccttcc gcctctttct
    21661 cagggcttgt cgtgaaaggg ggacggcgaa atacatgtcc ctcccacgga tacatcgtat
    21721 tgatgtaatg gatcttgtca taatttgcta attcaatatg actaggaaca gtgatttcat
    21781 caaaagaaca ggcatcaaaa tcttttttat aaaatgtttt tggtcgtacc gaaggatttg
    21841 gcgaataaca aaaacgccat gatccattta aagactgttt aagggaggat gtggtttcta
    21901 cttcttccct tgttgcataa caaacatgat cgctatgtgc atttgattgg ttcactctaa
    21961 aaacttgtgg atcatctaac cattttatat ttgctttcat acttttttcc ttctctctca
    22021 taactttttg aaatgaagca gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag
    22081 tccaactgtc atcaattcgg cccccgaaaa tatccgcttc gaggtctgtt cctgataaag
    22141 tgcctcatca tctaacccca tcagatatac ttcatggaac gctggttgag ctgaagctag
    22201 tatcctccct aaaaacacaa tagcttcatc ttgtttagga gacacaaaca tccatgcggc
    22261 aatattttct tcaaaaggac tcgccaaacg ataataatgg ccatactgga tcaccggacg
    22321 aatcgtttga taggatataa tttggtcttt tacttgcttt tcttcttcag gagacagctt
    22381 tgtcaaatcc agttcataac caaaaacagc gctcatagct gtttctcctc ttgttttcaa
    22441 cgaagtagat ctgtcggtct gatgatttgg tacagctgat acatgagctg tgatggacga
    22501 aatcggataa gcaaggcttg ttccatattg gattttcata cgctccactg catccgtgtt
    22561 atcacttgtc catgactgcg gcatataata aataaacccg gcatcaaacc gtccacctcc
    22621 accagaacag ccttcccata agatttccgg ataatcagtt gtcaaatgtt caagtagttc
    22681 ataaagcccg agcatataac gatgcgctac ttctccttgt ctctgtgggt caagggtaat
    22741 cgaatagaca tctgaaagcg aacggttcat atcccatttg atgtagtcaa gaggaatcgt
    22801 atctaaaatc tttctcattt gtttctcaat cgtctgccga acatctaacc ttgtaaaatc
    22861 cagtacatgt tgtgatctgg aagcagaagg cattctacct ggctcctgca taagaaaatc
    22921 aggatgtgtt cgatacagtt cagaatcaac cgagatcatt tcaggttcaa accataaacc
    22981 aaatttcaat cctttgctat gagcataatc tgctatttca cgtaatccat ttgtcaactt
    23041 gccttcatac tcgaaccagt ctcctaatga agaattgtcc gcatctcttt ttccaaacca
    23101 gccatcatct aatacgaaca tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt
    23161 gattttttca ctattgaagt caaagtaagt ggcttcccag ttattgacca gaatcggccg
    23221 ttcagcaaat tgatgacgac ctcttgcgat tctttcacgt agcaactcat ggtggatctg
    23281 actcattttg ttcaatccct gacttgagta agaaaggatc atttcaggtg tttgaaaaga
    23341 ctggcctgca ttcaattccc acgaaaaacg atggctgttg atcccaccga cgactctcaa
    23401 ttgattgatc tgatcttttt caagttcgaa agaatggttg ccggaataaa caaattgaag
    23461 tccgatcgct tctccagtat tttctgtcgt atgatgatga actaaagcaa taaagttatt
    23521 catatggtga ctgctcgttc ctcgacggct ttcaaatact tttgttccag ataaaacaga
    23581 ttgccgtgaa atctgtcttt cattgacatg tgctcctggc aaagcaatga cttcatcaaa
    23641 tcgcctagta tgggcaaagt ctagctggaa actagccgct ttttctagtt tgattgtttc
    23701 tccggtttca tttcttatct ttacagatcg tgtgatcact gctctatcag taaatatggt
    23761 atagaacaaa tcgaaataaa tcgctgtttc tctatcctta agtgaaatca ccaacgtttg
    23821 tgcctctgac ttgtccatca cataagtttg aggcaagcct tcgatctctg gcttcccatc
    23881 tgtaatataa tggctgtcat atctcagatc cagtaagtct gatccattct tgcgctgaat
    23941 gatcgatgcg ggcgttcgaa agtccatgct gccatgagaa gagtattctt gcggtagtgt
    24001 atctttcgac agagaccggt caggatttaa gggaacattt cctgaaaatc cacgatctct
    24061 tcggggataa tttttattgt ctttgtactg cttgactggt ttgccaaaat aaatatgcgc
    24121 caatacattg ctttcttcta tctttatcag ataagaaaga aatgtatttc gtaaatggaa
    24181 gactcggctc ttttcatcaa ataagatgaa atttttatct gtttttctat gcgtaatatc
    24241 catcatttca ctcccaacaa aacggaaacc gtttactttt ttttataatt cctgtatact
    24301 gtttactaaa caattttgtc aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg
    24361 gttggaatta gagatgtcgc acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg
    24421 ttgaataacg atcagacgat ccatgtggct cagactactc ggcaacggat agtagaggca
    24481 gctgctgcgt tggattatga catcagtact cgtaaatata tcaaaaagag aatgccttcg
    24541 attggtgtga tctctacgat cggcaaagcg agcgaagaac aggatatcta ttacaaacat
    24601 ttgcgagtag ggatggaaga agaagctcga cgtctgcatt tagggatgaa tcgaatctat
    24661 aatctatctg ataatcctaa acaatgggaa gatctagatc agttaggtgc ggtgatcgtt
    24721 gttgggactg ttacgaaaga atcgattacc gccttgttaa aacaaaacat gcaaatagtc
    24781 gtcgtggata accctgacat ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg
    24841 atgactaaaa ccgtactgga attgttctta gaaaatggtc ataaaagaat agcctatatc
    24901 gggggatata aagtcgatat ggatgaattc ggtgggaagc agatttctat aaatgaaaag
    24961 aggatgcgtg cctatcagca ttttatgatc gatcatcact tagaaaaata tatgtcttat
    25021 catctaggtg gctggacaga aaaacaaggt gaggtacttg cgactaatct attagaaaat
    25081 gaatcccctc ttcctactgc cattcttgtc gggagcgatc caatggcaat tggtgtctat
    25141 cgggcattga aacaaaaaaa tatccaaatt ggagaagaga ttgttgtagc aagctttgat
    25201 aatatcgaga caatcgcaaa cctctcccct ggcttgacca gtgttcaaat caatgcgaaa
    25261 tcaattgggc aagcagctgt tcgaatggca ttggagcgaa ttgacggtat acgggacgaa
    25321 ccaatcattt tgacttatcc tgcacgattg attgttaggg aaagttttat cccaagaaca
    25381 aaaaggtatt gaaagagagg ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa
    25441 cggctttttc acaacctctt acttatactt attcacaaaa tccaatgtct gctggtaaca
    25501 attttgcgat tcttttagcg tgtaatcaaa ttggtattca tgggtaatct ctttttttgt
    25561 atctgtataa aacaaggatt gcacgggtac ctttaattct gtcaaccgat tttctaatgc
    25621 ttgaccttgc tcctggaatg agtatgcatt tccgtcagtt atatacgttg gcggaaattc
    25681 cttagaaaca tggtcagcca cactagcttc tttcaattgc tcactcgttt tccaatcttt
    25741 tgttccaatc aacgaccacg caacggtttt gacgaaaaac ttcataaatc gatcatccga
    25801 atgctgctgt gctgttagct tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag
    25861 actatctttt ggaagtagct gtttgatttt catttgttta gcatacgcat cattcgtttg
    25921 tatagctgca tattgcaatg cgatttgtga acctgcacta tctccaccaa aaaataattg
    25981 ttccatgtct agcaatttgt cgtctttttc ttcagctaat aaatctttga tcagttcatt
    26041 cacttgaaga acttgattag ggtattctga atcaggcgct aattcataat tcatcgcaac
    26101 cactgcgatg ttggcgtctg aaactaattt tgtcgcaaat tctttgacac ccgatttatc
    26161 tccgccgacg aatcctccac catgtaccca aattagaaca ggaacaggcc ccttgctgtt
    26221 ttttggatag tagatgtcat aagtattctc tttatacttg gactcatacg tcaaatctgt
    26281 ttttttcgcc acgttttttt ctgcttgatc gaacgttttt ttatcagtga tttgaacagt
    26341 atttgaaaac atatggttga tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag
    26401 aaagattgca aacgcgatcc caagaatgat tccgaagaac catccgataa atttaagaat
    26461 tttctttttc ataaggctcc ctcagtagtt agtattaagc attctgatta taccatagtg
    26521 ttatttccta tcaagctctc ctagatgatt taatgatttt ccccaaactt cttctgcaat
    26581 gcttttgaca aaagaaattt tttccattgt tcttcaaaca aaacattttc ttcttctgtt
    26641 gaagcaaatc cacattgagg acttaagcac aaacgatcca aaggcagata tcgtgtcgct
    26701 tcgtagatcc ggtcaatcac ttgctggtgc tcttccattt cgccattttt cgttacagtc
    26761 aaaatctaag acaactaatt tatcttcaga gactttctct aaaagaacaa aactcccagc
    26821 acgttcacta tcgtattcta aataataggc acggacattt tcttgagtaa aataatgggt
    26881 ctgctacgtc gtcatccccc ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac
    26941 atgtatattt agtgttgaat catttgagaa tatgaaaata aaaatctttt tgatcaatca
    27001 gttaatcaaa ttgaaataac gaaaaatctg agacagaatg acgttcattc tgacccagat
    27061 ttgattttgc acaactcaac agtccttatt ggcaaatcgc caaaataatg tagactaact
    27121 aaaaagacta agaaacaata acaaaagtta acacttctta gtctttcttt agcacgtcta
    27181 tttattaaag attttagggc cttttctcat gtttttacga tcattcttag gtgtttccat
    27241 ttgtttggat cggcctccac cttgtttctt tttgatcaat tctttcattt tttctttttg
    27301 gtccattttt attctccttt aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg
    27361 aaaagatcta caggctgaac tttcttcacg gcataacctg cttctgcaaa taacttagca
    27421 tctcttgcaa aagttgccgg attacatgag atatagacga ttcgttctgg tgacatttcg
    27481 attgctgatt caataaaatg atgatctaat ccttttcgtg gagggtcaac tacgatcaca
    27541 gatggtttga tccctttttc ttgccatttt ttcataactt tttctgcttt tccgacttca
    27601 taatgaacat tttcgatttt attatttaaa gcattgaact gtgcatcttc aattgcttct
    27661 ggaataactt ccatcccata caccttatcc actctgtctg ctaaagacaa gccaatcgta
    27721 cctattcctg aataagcgtc gatcacaatg tcttttttat ctaactcggc aaactcgatg
    27781 gctgtttggt acagatactc tgcttgttga gtatttactt gataaaatga ctttgctgag
    27841 attcgatagg tttttcctaa tagctgatct tcgatatagt ctctgccata taaaactttt
    27901 tctttgtctc ctaaaatcac atttgttttt tcttcattga tattttggat gaccgaaacc
    27961 acttctggca attcttcatg aatagcttca gcaatttcct tggatttaaa gaatttttct
    28021 ttacgagtaa ctaaaacgac catcatttca tgggaataat gaccacgacg gataatgatg
    28081 tgacgaagct gtccttcatt tttttcttca ttatatcctc gtacttggaa ccgttgcaag
    28141 atatctctga ctactttgat tgcttgatcg atgacaggat cttggatata aaaatcttcg
    28201 atagggacta gatcatggct attctttttg taaaaaccgg tggctaactg tccatcgata
    28261 cggcgaacag ggatctgtgc tttattacgg tagccgaatg gttctgccat tccgaccgtt
    28321 ggaagtacag gaacttctgg catcttagca atcttcgcca ttacatgctc tacttgtttt
    28381 tgtttgaaga caagctgttg ttcatacttc aaatgggcta aaggtgcgat acctatgcga
    28441 agtaatgcat tgttttctac aggctgacga tcaggtgatt gtgtaataaa tgtttcgact
    28501 tttccatatc caaacttatt gcctgctttt actactagta tttctacttc ttctccaggg
    28561 agtgcatttt cgataaataa tggataacca tcgatttttg ctacccccat tccttcataa
    28621 ctcaagtcga cgatttcgac tgtatatcgt tcgtttttct ttactatttg ctgttctgtc
    28681 atattttgct cctttaagcc tatttttcaa tcctactgaa ctctactata aatcagctaa
    28741 ttacacaact aaaaccggca ctgaccttac tacgttcagc accggctttt gattaattct
    28801 tttgattagg tgtttcgtct gctatttttc cgtcaccgtc gatatcttca tccgttaaac
    28861 gttcgacttc ttttacgaat tctttgctga caacctctaa ttcttcttca tctgtacctg
    28921 tgattgcttc atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat
    28981 tttggaaatg catcggtgct tccccgccgt attcaccatc taagttgatc attactggtc
    29041 gattgatatc atcttctgtt tcaacgaaca aactgcttgt tttggtatag attattcttg
    29101 gatcatcgac atgtcgaccg ccattcagca tgagtgctgc aatatgcaag atctcaaaga
    29161 tatttgctgt cttcacaatg attaaagaaa acttcccatc atccaatttg gcatctggcg
    29221 cgatctgttc gaatcctcca acagagttcg tcagccctag gaaaaacatc gaagcatttc
    29281 cgatatattc cccttcatca tacttcaagc gcattttgat tggctttact cgagggagca
    29341 tctctgctcc tttcgcaaga taagcaagat aaccaaaaat actctttagt tctgatggaa
    29401 cttcataagt caattctgtc aaatagccac ctgcagcgat attgatgaag taactgttat
    29461 cagaaaggcc gatgtccatt tttacggttt ggtgtttttt tatcacttct gctgctgctt
    29521 taatattatc tctaggaatt tttaatgctc gagcatagtc gttcgttgta ccagctggga
    29581 tgatggccat tttaggacgg tgttcaagcg gagcgattcc attaaccact tcgttgattg
    29641 tgccatcgcc accagctgcc actactagat caaaacctag ttctgcgact ctacatgctt
    29701 cattcttggc tgaatctggt tctggtgtag ttgcataggc gctggcttca aatcctgctt
    29761 cttctaatgc ctgtaggata tccgccaaat tttttttcaa taattctttt cccgaggtgg
    29821 ggttataaat tacgcgtgct tttttcattt tcagcctcta tcgtttcgct aattcatctt
    29881 taagtagctt attcacgaca ccaggatttg cttgtccttt agttgctttc atgatttgac
    29941 cgactaggaa gccaaccgca cgatcttttc catttttgaa atcatcgaca gattgttggt
    30001 tgttatccaa aatttcatta atgattggca gtagtttgtt cggatcagat aattgcaccc
    30061 aaccattttt ttcaactact tcttgggctt ctccgccatt tgtgatcaat tcacggaaga
    30121 cttttttggc gatttttgag ctgatcgttc catcggcgat caaacgaatc attcctgcta
    30181 agttctcagg tgtcaatttt gtctcagcta attccagttt ctcactgttc aagtaagcag
    30241 aaacttcacc catcaaccag ttagatgctt gcttggcatc tgcaccattt tccaaaacag
    30301 cttcaaaaaa atcagacatt tcttttgtaa gtgtcaatac cattgcgtca tactctggaa
    30361 gtccaaactc acggatatag cgttcgcgac gagaagctgg catctcaggc aaactagctt
    30421 ttactttttg aatccattcg tcatcgatca cgaatttcgg gatatcaggc tctgggaagt
    30481 aacgataatc actagaccct tctttgacac gcatcaaaac tgttttattc gttgtttcgt
    30541 caaaacggcg tgtttcttgt tggatttctc ctcctgaaag aagaactttt gcttgacgtt
    30601 tttcttcaaa agctaaacct tttttcacga aagataaaga gttcaagttt ttcaattctg
    30661 ctttcgtacc gaactcttct tgtccataag ggcgaagaga aatattggca tcacagcgca
    30721 tagagccttc ttccattttc acatcgctga cttctgtaaa ttggataatt gatcgcaatg
    30781 cttctagata agcgtatgct tcttctggtg aacgcatatc agcttcagaa acgatttcga
    30841 tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata accgccaata ccatgcatgt
    30901 tttttccagc atcttcttct aaatggacac gttcgatccg gatttttttc tttttacctt
    30961 ctacttcgat ttcgatccat ccgtcatgac cgattggttg atcaaattgg gaaatttgat
    31021 atgcttttgg attatctggg taaaagtaat ttttccggtc aaaatgagta tctttagaga
    31081 tttcacagtt taatgccaaa gctgctttca tcccgaattc cagcgcagct ttattcatta
    31141 ctggcagcac gcctggataa ccccagtcga tcacgtttgt attgctgttt ggttctgcac
    31201 caaaatgagc tggcgctgga gagaaaattt ttgagttggt ttttaactcg acgtggactt
    31261 ctagtccaat gactgtttca aaattcattc gtctttccct cctaaaatag ctggttgttg
    31321 tttatggaat tctgttgctt gttcaaacgc atatgccgct tgatacatcg ttttttcatc
    31381 aaaataatta ccgatgatct gcaatccaac tggtaatcct tctgataaac ctgccggtac
    31441 agacatacct ggaagtccag ccaagttgac agggatcgtt aagatgtcac tcatgtacat
    31501 tgtgattgga tcgttaatgt tctcaccgat tccgaatgca actgttggtg aagatggccc
    31561 gatgatcaag tcataatctg caaagacttt ttcaaaatct cgtttgatca atgtacggac
    31621 ttgtcctgct tttttgaagt aagcgtcata ataacccgcg cttaaggaga atgtacctaa
    31681 catgatacgg cgttttactt cttcaccgaa tccttctgaa cgtgagttga catatacatc
    31741 atctaatgat ttcacatttt cagagcgata accgtagcgg ataccatcga atcgttgcaa
    31801 attggaactt gcttctgatg aggcaatgat ataataaaca gccacaccgt attttgaatg
    31861 aggcaagctg acttcttcca caacagcacc taatgaacgg aatgtttctg ctgctttcaa
    31921 tacagcttct tttacgccag gggccacacc ttcgcctaag tattctttag gtaaagcaat
    31981 cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga
    32041 agaagtaccg tctttttcgt cataaccgct gatagctgtc aatgctaatg cattatcttt
    32101 caccgtacgg gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa
    32161 acgtgagaca cggccataag taggtttcat ccctacgatg ccgttaaaag ctgctggttg
    32221 gcggatactt cccccagtat cactacctaa agaaactgga atttgtccag ctgcaactgc
    32281 tgctgcagaa ccaccagaag agccaccagg tacttttgtt gggtcccaag cattttttgt
    32341 tttcttgaaa taagaagttt ctgtacttcc acccatagca aattcatcca tgtttagctt
    32401 accaacaggg atcatatctg cttgatagat tttatccatg accgttgcat cataaattgg
    32461 ttcaaaattg taaaggatct tagatgcagc ggttgtcaag atatcttttg ttacgatatt
    32521 atccttgata ccaattggat taccagcaag gacgttgctt tcatcaatcc ctttttcatc
    32581 taaagctttt gcctgttcca atgctttttc ttcgttcaat gtaatgaatg cctcgacctc
    32641 aggctctgtt tctttgattc gttcaaatgt tgtttttgtc aaatcttgta cagtgatttc
    32701 tttcgataca agcatatgat gcagttcttc tagagaacga ttgtgtaatt ctgtcattat
    32761 gcgccagcct ccccattatc gatgattgcg ggtactttga taaagccgtc ttctgtttcc
    32821 ggcacatttt tcaataattc atcgcggttc catccagctt gcgcccgatc ttgtcgcatc
    32881 acgtttactg tttcaaccac attcgacgta aatggaacac cttccgtatc tacttcttcc
    32941 agcatttcaa ccatatcgat gattttacct aattggtcgg taaaacttgc aagttcttca
    33001 tctgcgaacg caagttttgc taatttggca acgtgtttca cttgttcttc actaatcgcc
    33061 atactacccc ttctttctaa acgagttaaa aactttgctc tctattttaa cacaagagtg
    33121 ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac tcatttttca ttcttctact
    33181 gagcgacttt tagccaatgc atctgccatt tgatcttctg tccaaacttc gattcctaat
    33241 tcttgtgctt tggtcagctt gctgccagca tcttctcctg ctaccacgat atccgttttt
    33301 ttcgatacgc taccagtcac tttcccgcca agattttcga tcgtctcttt tgcttcttca
    33361 cgagtaaatc gtgttagttt accagtaagg actactgttt tttctttaaa aggagactcg
    33421 acttcttgca attgggcatt tcgaagccct ttgtattcga aattgacgcc tgcttgtttt
    33481 aattcattca tcaattcatg gacttcttcg ttactgaaat aagtgactac actgtcggca
    33541 atgatatcac cgatcgtatc aagcgcaata atcgactcat agtcactttt actcaatgtt
    33601 tctagatcac cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac atgtcggatt
    33661 cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa
    33721 atattatttg ctgatttttc ttttattttg tccaacgtca ataactcttc ttctgtcaat
    33781 ttgtaaagat cagcaacatc tgctactagt tttttatcat acatctgttc tagtacgcgc
    33841 ggccccaacc catcgatatt catcgcattt cttgaaacaa agtggttcaa accttctttc
    33901 atttgagcag ggcatttagg attgatgcac cgtaaggcta cttcttcatc caaatggaca
    33961 agttcacttc cacaaactgg acaatgtgta ggcaattggt attcttcgct gtctttaggt
    34021 cgtttatcta acacgacttg cgaaacttct gggatgatgt ctcccgcttt ataaatcaga
    34081 actgtatctt tcaagcggat atccttcaac ttaatataat caccgttatg caggctggca
    34141 cggcttactg tcgttcctgc tactcggact ggtgtcatga tagctgttgg cgttactacc
    34201 cctgttcgac cgactgtcca ttcgatgttt tcgattagcg tttcaacttc ttctggcgga
    34261 aacttgtaag ctgctgccca tctcggtgct ttgatagtaa aaccaagttg atcttgtagc
    34321 gagaattcat tcactttgat gacgattcca tcgatttcat agggcaaatc cacacgtttg
    34381 tcatgatatt cttcaatgta tgaccacact tcatcaatcg aatgacatag acgtttttcc
    34441 cgattcgtgt ggaatccaat tttttctagc tcttctagcg catcatactg ggtttttgct
    34501 tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg
    34561 attttagaat ctaactgacg caaagaacca gctgcggcgt tccgaggatt cgcaaagata
    34621 tcttttccct ctgcttctct gtcttggttc aattggacaa atgaccgttt tggcatgaaa
    34681 cattcgcctc ttacttcgat gttcattggt tcttttaatt tgattgggac cgaccggaca
    34741 gtttttaggt tttcagtgat gttttctcct acagttccat ccccgcgagt cgcacctctc
    34801 acgaaatttc catcttcata acgcaaagaa acggaaagtc catctatttt tagttcacag
    34861 caatagtcaa ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc tatcagctct
    34921 tctttgctga aaacgtcgtt caaactgtaa agcggaatat catgagtgac tttttcaaaa
    34981 ccttctaata cttttccgcc tactcgttga gtgggagaat cagacgtgat caaatcagga
    35041 tattgttcct cgattgctac taattctgcg tattctttat cataaacata gtcttccact
    35101 gttggtttat cttctacgta gtattcgcga gaccattggt tcagtcttgt acgaagttct
    35161 tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt ccatcattta ccctccacta
    35221 ctaagctttt tcaattggcg caaaggcagc caaaagacgt tttactcctt gacttgggaa
    35281 agccacatct agttctagat ccttcgacgt cccgcttact cggacgactg ttcctagacc
    35341 ccattttttg tgtttgactt tatctccagg tttccagctg ttattttccc ctccagtagc
    35401 acttttagag ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg tttttttagc
    35461 gaatacaggc gtttttttag gcgtaggacg catgccttcg atttccagta actcctgatc
    35521 gatttcttct acaaaacgag aaggtcggtt atattgcgtc cgtccatata atgttctgga
    35581 gaaagcattt gttaaataca acgcttcttc tgctcgcgtg atccccacat atgcgagacg
    35641 gcgttcttcc tccagttcgc tttcttccat caatgctcga gataaaggaa aaaccccctc
    35701 ttctagacca atcaggaata caactggaaa ttctaatcct ttggctgcat gcaaagtcat
    35761 caatgtcact tgggaagcat cttcttctaa attgtcaatg tcggaaacaa gtgccagatc
    35821 atttaagaag accgttagtt tttcttctgg tgcatcagcg tcttcttcat tttgctgttc
    35881 gaattgtttg tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc
    35941 ttgtgcttct aatgtatttt ggatttttaa gtcttctaga tatccgctac gatccaaaac
    36001 ctctttcgtc aattctgtca ctgtcaagta tgggatcatc tgagtgactt cttggatcat
    36061 ctctccaaat gcacctaatt gctgtcctgc ttttccaccg atatttgcca gatccacatt
    36121 ttgcgctgct tcaagcagtg gccattcatg aagagaagca aaactgcgta gtttttcgat
    36181 agatcctggt ccgatgcctc gtttgggcga attaacgata cgctcaaaac tgattgaatc
    36241 ttgaggattt accagcacat tcaaatacgc taaaatatct ttgatttctt tacgatcgta
    36301 gaacttgtgc cctccaacca ttttataagg aatatttgct ttcaacagag tttcttccat
    36361 tacacgagat tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat tccgatgatt
    36421 gcttcgaatc tcttcttgca tccggtctac gataaaacgg gtttcgtctc gttcattatc
    36481 tgcacggtag taagtgatct tgtttccttc tttgttttct gtccacaagt tttttggttt
    36541 tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg
    36601 atagttttgt tctagcaaaa ttactgcagc atctggataa tctttttcaa aatccaagat
    36661 gttctgcata tctgcaccgc gccagccgta aatactttga tctgcatctc ccacgacgca
    36721 caggttccta aatctacctg ccaataaatt gactagcgta tactgtgcgt gatttgtatc
    36781 ttggtactca tctacatgaa tataatggaa tttgttttga taatacgtga gtgaatcagg
    36841 atgttcttca aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca tacattgatt
    36901 atattgaagc tctttttggt aagcagcata acattttgcc gcaatctctt cgtaataact
    36961 gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt gagagattgt
    37021 tcctaatata cttcgaggat catatttttt cggatcaatg ttcagttctt ttagtatccg
    37081 tttcatcaag gtcagctgtt cagaagaatc gatgattgta aagttccggt tatacccgat
    37141 aaaatcaaca tctcttctta aaatacgcac acacatcgag tgaaaagtcg atacccagac
    37201 atcttcgcct ccagatgcga gaatcgcatt gacacgttct ttcatttctt ttgcagcctt
    37261 attggtaaaa gtaatcgcta ggatgttcca aggattgact tctttttcct caatcaaata
    37321 ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct gcacctgcca ttagcagtaa
    37381 tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt ttaataattc
    37441 agctttggat gacattaaca acatcctttc ttttatcaat cttgttaatt atagcatttt
    37501 cttggctttc attaaactag ctagttcaaa atcatttgtt tctttttatg gatttttcct
    37561 tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa
    37621 aggaaaaatc aagaaattag ataagactca gatatatttt attctaaaag gaatatttgt
    37681 tggactgctg acaggaatcg tcgttagcct gtttcgatta tctatcgaaa agctaagcga
    37741 tttagttcga agtatctatg aaatgagtcg ggaacagccg atttacttaa ttggcctagc
    37801 tatttgctgc atccttgcag catttttcgt gggttatctg gtcaaaaatg aaccggatat
    37861 caagggaagc gggattccac aagtagaggg acagcttcgc ggggaattat caatgaactg
    37921 gttctctgtt ttatggaaaa agttcattgg cggtgtactt tcagttggtg cgggcctttt
    37981 tctaggtaga gaaggacctt ctatacagct tggcgcatcc attggacaag gagctggaca
    38041 attgtttcgg agtccatctt cagaagaaaa gattttaatt tctagcggtg caagtagcgg
    38101 acttgcagca gcattcaatg ctcctatcgc aggattactc tttgttttag aagaaatcca
    38161 tcactccttt tctccacttg tttggctgac atcatttgct tcagcaatca ctgctaactt
    38221 cgtctctctt tactttttcg gcttgagacc tgttttatat ctaggtgacc ttccttcttt
    38281 gccgttgaaa tattatggtt ccttagtgat cttaggagta cttctaggca tcttaggttt
    38341 tatttatcag aatgtcttgc tggctttgcc aaaattttac aaccgattgc ctttaccagc
    38401 ttattttcac gggttcgttc ctttcttgct gattttgcca attggttatt tatggcctag
    38461 tactctaggc ggaggcaatc aaatcgtttt agcttttgga catgcttcct tgccaatcat
    38521 tatgattatc ggtttgtttg ttcttcgatt cgttttttcc atgatttctt acggtggggg
    38581 tcttccaggg gggattttcc ttccgatatt gacattgggg gctttaatcg gtagctttta
    38641 tggaaacatt atggttgatt tcgggatgga tccaatctac atcaaggatt ttgtcgttat
    38701 tgccatggct ggttatttca cagcgattgg aaaagcaccg ctgacagcaa ttattttagt
    38761 gacagaaatg gttggctctc ttaatcatct gatgccttta ggattggctg ctcttgttgc
    38821 ttatatcgtc aatgaccttc tcggcggtaa tccaatttac gaatcattat tggaacgtct
    38881 acttggaaat actcagaccg cctcactaaa aggtgcaagg acaacttttg attttccagt
    38941 aacggcagag agttctttag acggattaat ggtacgagac ttcaattggc ctaaaaatat
    39001 gctgctaatc tctatacgca gaggaaatca ggaaatcttg actcacggtg atacgatcat
    39061 gaatgtagga gatattttgg ttatcttgac tgatgaagca aacttgccaa gaatcaagca
    39121 ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa tcattttaac gaacatggaa
    39181 aaactatttt acaacaacta ttcatctaca ctacttgatt ctatatgatt ctataaaaaa
    39241 agaagaggct gggaaaaccc agcctcttct tttttatttc tatgcttctg ctactgcagg
    39301 agcttttctc aataaaccat aaatcacacc agatacgact gctccgatag caataaacac
    39361 caaatacatg accgggtgac tcagtaagaa tacaacgaaa atcccgccgt gaggagccag
    39421 taatttgatt ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa taataaaact
    39481 tggaatggca cgaatcgggt cagctgctgc aaatggaatc gatccttccg tgacaaacga
    39541 tagtcccatc acgatgttcg tcaaaccagc atcttgatct gttttgctga atttattctt
    39601 aaacaaacgt gtagcaacga aaattgccaa tggaggtacc attcctccag ccataacaga
    39661 agccatcacg acacttccgc cagttgcaac agtcgtagct aaagttgctg taccaaacac
    39721 ataagcggct ttgttgattg gaccgcctaa atctgccgcc atcattccag ctaataatgc
    39781 accaagtaaa gcagcattcg aaccactcat acttgtcaag aaaccgttca atgcatcatt
    39841 gattgctttc atagggacat tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa
    39901 cactggataa aagaggatcg ttttaattcc atccaatgat tttggtaagt tcttgaataa
    39961 tttacgtaaa aatacgatga cataaccagc taagaatcca ccaatcagtg cacctaagaa
    40021 tcctgcaccg cctgtattag ctaatgcacc agcagcaaat ccaacgatca aaccagggcg
    40081 gtcaccgata cttgaagcaa taaatcctgc taataccggc agcatgaaac caaatgcagc
    40141 ttgtccgatt tggttgaacc agctagcagc ttgattgtaa ttccctagat ttgataattg
    40201 atcatgtggc acgccaataa attggtcgat catgaaagaa agcgcaatag cgattcctcc
    40261 accaataacg aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt aaattctttg
    40321 tccgatagta ccatccgcat ttccttcttc tgaactgctt tcttttccat caccatggaa
    40381 tactggtgca gtaccactaa gggctagatt gatcaactct tctggtttac ggataccatc
    40441 actgacagga cgattgatca gttcttttcc gtcaaagcga ttcatctcta cttttttgtc
    40501 tgctgcaacg attacacctt ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat
    40561 tccttctgaa ccgttcgttt cgactttgat ctccacaccc atttctttcg cttttttctt
    40621 caaagcatct tctgccatgt aagtgtgagc gatccctgtt ggacaagctg taacggcaac
    40681 aacgaatttt ctttctggat gagcagaagc ttctgcagct tgtgtttctt gttctgcttt
    40741 ttcttctgct tctttttgct cttcagctgt ttggaacaag tcttggactt cttctggtgt
    40801 atttgcttct tttaattttc ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc
    40861 taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga aaaacagata
    40921 agttggctgt ccgtctaagg cttcataatc tactcctttt tggctttttg cgaataaaac
    40981 tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg gcgattccat caccaagacc
    41041 tgtagaagtt tgtgcttcgc gggctagaat tccttcttta tatgtttcga tatcggaaat
    41101 ccggccggca tcaaacattt tttggaccat ttcatcgatt gcgccttttt tatcagtcgc
    41161 ttgcaaatcc atgatcatcg cttctttgac cattaaatcc ttgatattca tgattatcct
    41221 ccttgaaact aatcaatttt tgtgatttca acttctggca gcaactcatc aatcaattcg
    41281 cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag
    41341 gcttcgattg gatctttcgt acggctgtaa gccccaataa agccagcaat cattgaatcc
    41401 cctgcaccta ctgaattttt caatgggcga actaatacat tcgttttata agttccttgt
    41461 tccgtgaaga gcaaagcacc gtctccagcc attgagatca gcacattctg tgccccttca
    41521 gctagtaatt ttttcccata cggaatgatt tcttctacag attggaaggt tgtgttgtaa
    41581 agctcagcta attcgtgatt gtttggtttg atcaacaacg gtttcttaac aagtgcattt
    41641 tttaggtcat cacctgttgt atcaatcacg aaatctgcac ctttttctcg aatgatatca
    41701 atcagttctt gataaaatcc ttttcgcagg ctagctggcg cgcttcctga caatacgacg
    41761 atatctcctt gtcctactgt tcctaatgct tgcttcaact catcgatttc ttcatctgaa
    41821 atagatgggc ctaagccatt gatctcagtt tcagtgtctg attttagttt gatattgatt
    41881 cttgtatctt cttttacggt tgtgaacaaa gactggattt gttctttttc aagccacttc
    41941 gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg agaatcgatc
    42001 cgtttcaaaa tacgggaaac attgatccct tttcctccag gtaacttgaa gtcttccttc
    42061 attcggttca aatcgcctag ttttaaccca tctacgcgta cgatataatc aattgatgga
    42121 ttaagtgtta ctgtgtaaat cattgtattg cctccttaat tgttgtcttt tcacggaatt
    42181 cattaacgaa ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa
    42241 cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat caatacaaat gtctcttcgg
    42301 aatttttcat ggctaaacgt ttaacagctg cctcttctgg atccggagtc gtgaatcctt
    42361 gttcaagatg ggcaccgttc attcccataa atgctttatt aaaatgaaaa ttttttagtt
    42421 gttctatcgc attagatcct aaaacagcat tagttgccag ctttatcgtt ccacctaaaa
    42481 taatggtagc aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta
    42541 ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta
    42601 aataaatgac atcttcgtca ttaatggttt cagctgccga tttggcgatt tgctgttttt
    42661 cgtgaacgtt tttgagtgat ttttcactca tattctgctc aaaattcaat aattgctctt
    42721 tcttagctcc gccatgaact ctttctagaa acccactatt ctccagctct tgtaaatcac
    42781 gtcttatcgt tgattcagaa gcattcaaaa ggtttacaag atcttgtgat ttcacgattt
    42841 tctgctgttc aagcaagtct aggatttttt gtctgcgttc ctctgtaagc attttcacga
    42901 tcacctcaat acaaataata gcatattttt tttctactag caatataaat cagtcaaaaa
    42961 cgttcataaa agttataaat cagtcaaaaa aaaataaaaa accagtcaga aagactggtt
    43021 ttttattttt ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt
    43081 tttcatataa acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat
    43141 cccgccaaac atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa
    43201 gagacagacg gataataatg atgccggaac cattattcga taatagagtt gtttatccat
    43261 tttcttcacc tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa
    43321 attgcacacg cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt
    43381 tctttattcg tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt
    43441 ctgacaggga ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt
    43501 catctgttac ctctacttct agatattccc taatataata acaataagct tcgattccat
    43561 tatccaaagc aacagtcgga tcgataactt tatagcacat catgcgtgat cgattctcgt
    43621 caacccaagg aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat
    43681 ctccttgttg gatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacag
    43741 gttgcatttg tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt
    43801 caacgaatgg cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa
    43861 gctggtccct aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt
    43921 ctcgataaag ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt
    43981 ggatgtcgag aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt
    44041 cccaacacga agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc
    44101 aatcgactac tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct
    44161 ttttctcaaa caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat
    44221 aagctcgcat ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa
    44281 tcaaaaattg cttttctttg aaaacagaga tagacaatac attttttatc aataattgat
    44341 tatcttccaa gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt
    44401 ctcttaccga tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt
    44461 gttgtgtgaa agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat
    44521 cgcttatttc ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata
    44581 atttatcttc gccaatcatt ttctcacctc tttcaaatct ttttataatt taattataac
    44641 caatgataaa gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat
    44701 agactaagta aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga
    44761 taatttagat aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa
    44821 agaagagatc gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa
    44881 acaaaagtaa gttaagctca aaaaaagact taccagccaa acttttatga aagagtttga
    44941 ctggtaagtc ttttttattc tttttatgat tcaattgctt gttcgattgc tgcccacgct
    45001 tcttcttttc cctgttttgt aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa
    45061 tccaacgctt ttttgatttc gctctcatgc ttgttccatt taccacgagg gatcttatct
    45121 gccttagtgg caacaacaat tacagggata tcataatact tcaaaaattg atacatctgg
    45181 atatcttctt gagaaggctt atgacgcaaa tctactaaag aaacgacagc acgtagctgg
    45241 tctctagtcg ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc agttttggat
    45301 acttttgcat acccatatcc cggaacatcg acaaaatgta atgcgttctc gatcaaataa
    45361 aaattcaatg tctgggtctt tcctggctta ccggaagtac gggctaaatt ctttcgatca
    45421 atcaatgtgt tgataaaaga agatttccct acatttgatc gtcctgccag agctatttct
    45481 ggcaattgtg tatctggata ttgttttggt gccactgcac ttatgataat ttcagcttga
    45541 tggactttca tggtctctct ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa
    45601 agccaaaatg acttttacgg cagcctcttt tcatcaaccc gcttttttct ctgaatcttt
    45661 atagattact gttggctcac cagacaattc agctgcttct tttgtgataa tcactttttc
    45721 gatgttttca tttgaaggga tatcaaacat cacatccatc atgatttctt caatgatgga
    45781 acgaagtcct cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc
    45841 ttcaggttcg aattcaagtt cggtattatc tagagaaaga agtttttgat attgtttgac
    45901 tagcgcattt ttcggctcag tcaagatccg taccagatca tctgttgtca atttctctaa
    45961 agctgccata acaggtaatc gtccgataaa ctcagggatc aatccgaatt ttagtaaatc
    46021 ttcaggaatg atatgctgca tgacactttc gtcttcagaa agtttttggt tttgtgtacc
    46081 aaatccaatc gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc catcaaatgc
    46141 accacctacg atgaacaata tgttcgtcgt atcaatctga ataaattctt gatgtggatg
    46201 tttacgtccg ccctgaggtg gcacacttgc aacagttcct tctagaattt tcagcaaggc
    46261 ttgttggaca ccttcgccgg aaacgtcacg agtaatagat acattttcgc ttttacgcgc
    46321 aattttatcg atctcatcaa tatagatgat ccctttttct gcaagttcca cattgtaatc
    46381 agcagattga agcaatttaa gcaaaatgtt ttctacatct tcccccacat aaccagcttc
    46441 tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc aatgtttttg ctaatgtttg
    46501 tgctaaaaat gttttaccag accctgtagg tccgatcaag cagatattgc ttttttgcag
    46561 ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt gattgtatac
    46621 ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat tcaatacttc
    46681 aagaatttct agtggtttag ggacttcagt gaattcgcgt acagcttctt cataaaattc
    46741 ttcatcaatg atttctttac ataggtcgat acattcatta cagatgtaga cccctggacc
    46801 agctacgatc tttttcactt cttcttgggt tttcccgcaa aaagaacagc gtacggtctc
    46861 attgctactt ggattgtcat acatggtctt caccccttaa cttgcaaaaa gtatgcaaga
    46921 aactatcaaa gactttacag tcttttacta tcataacata gtttagtaaa aagaaaaagt
    46981 gtttaacaaa ggatcaactg acctatacct ttgcattttt tactcttaac aaaaaataag
    47041 gaactgtgac aaaatcgtgt cacagtccct tatttcgaat aaacaatttt ctagaagtag
    47101 cttcttcaga aatgatttat tacttgattt ctattctttc gcttctgtcg ctgtacctgt
    47161 gatcaattcg attgcttttt tcattgtcac atcatgttcc agcatatctt ctgtcaacac
    47221 gcgtttgatt tgttcgattg gcatgttgta tgtttctgat aattcttgga tttctgcatt
    47281 gatttcttct tctgttgctt gaagattttc agcagctgca attgcttcga tcacaagatt
    47341 tgttcttgtg cgcatttctg cttcaccttc gaattgttta tgcaagtctt cttctgtaga
    47401 accagttaat tgatagtaca tttcaggaga gataccttga cgttgcatgt tgttcaagaa
    47461 ttcgtccatt gaacgatgta cttcatcatg aaccatcacg tgtggcaatt caacgatttc
    47521 tgcgttttca acagccatac ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc
    47581 ttttgtttct actaattctt tgcgatattt ttctttcaat tcgtctaatg ttgatacttc
    47641 gtcatcaaca tcttttgcga attcgtcatc taattcaggc acttcttttg cttttacttc
    47701 atggattgtc actttgaaga ctgcttcttt tccagcaaga tcttctgctt gataatcttc
    47761 tgggaaagtt acatttacat ctagactttc acctgattta tgaccaacta attgatcttc
    47821 aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag taattttcgc cttttccgcc
    47881 atcaaacgct tcgccatcta caaagccttc gaagtcgatt actactgtat cgccattttc
    47941 agctgcagca tcttctttga taactaattc agcttgtgct tcttgttcac gtttgatacg
    48001 tgcatccacg tcagcatctg ttacttcacg gtcttgtttt tcaacaacta gatctttata
    48061 ttgtcctaat tttacttctg gtttcactgt tacttcagca gtgattaccc agtcttgacc
    48121 tttttccatg ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt
    48181 gacagcagct tcataagctt ctggtaatac agcattcaat gcatcttcat ataaagcttc
    48241 ttcaccgtac atacggttga atacttgacg agaaactttc cctttacgga atccaggtac
    48301 gttcaagctt tttttcactt tattaaacgc agttgttaat cctttttgga tttcgtcttg
    48361 cgcaatcgta aattttaaaa caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat
    48421 ttaattccct ccgagtttag aaattcattt tatacatcaa aaacgtttca tgcatacctt
    48481 taaatagtac actaaccctt tcaaattgta aacatattga atggatagaa tccttgtttt
    48541 tcataacatt tttatctttt tcactgataa aatctaatgg aagaagatca tttgctgcaa
    48601 aattttttct ctttttttta aaaaatcttg tttttcatct tctgtacctt catacatcgt
    48661 ttttcctgta cacgcctcta cagtcagttg aaaccagcgc ttcgggtctt cgatgtatgt
    48721 atcatagaca ggataaacaa ttgcagattg caacgtgaat tcttctttta acacggaact
    48781 aagcagcgca tcttcattcc cgtaacgatc tgaaaataca cataattgct ggtaacttgc
    48841 ctgctgctgc ggctttggca gatcttttgg tatgactttg actaactttt cttcaatcgt
    48901 caaatagaaa acaggatggc attcgttcaa ttgcctaagt gtctccagta attttgccct
    48961 tgctaaaata tggacctctg gcgatacaat aagtttactc gctgtttgga taaactcatg
    49021 ataaggaagc tgttcgatct catcaataga cgcaagctga cgtgcaggtt ccaacgaagg
    49081 aagttcctcc gatttattgt ggagtgcctt gatcttctgc ctctccattt gtccggataa
    49141 ttcttctgca aattggattt tttcaatgac ccgctgctcg aaagcttcat aaaaatctgt
    49201 acttgctatc aattttctag catataaaaa atcttgtgta ctgattaaca agtcaaaata
    49261 aaattcagca aactcctcat tttgaagata ctgtttttca tgcaagagtg cttgcttcaa
    49321 tgcttctttt ttctcgtcca actcgtaaag acaaaaaaca agcaggctat tagcctcaaa
    49381 gctttctttc aactcgtaag ctcgttgaaa taattctttt gcctgctgat attgctggtt
    49441 tgctagtgct tgttgtgcca ctcgcattaa attctcatat ttttctggaa aatcaatcgg
    49501 cttcacgcag tgtcctcctc ttgctcatat tcagctcatg ttcaatagta gcaaaacaaa
    49561 aaaagcatga caattaaaaa taactgaagc accctctaaa agctagattt tagatctgac
    49621 ttttagaagg tgctttcaac tgttactgct ttttattgct catcagctgt tgacttcctg
    49681 gttgaacgct ttgatcagat tgatcgtaac aacacttgta ttagtgaaaa aatcgcccag
    49741 atgttgcttt ttctgattaa agactgctgg aagataccct aacatcagca aaggaaattt
    49801 tagaatgaac cggcaagcac cttctctaac taaaacggtt gaccagctaa gttcttcttc
    49861 atcaaagctg atgactcgaa taccaaagat cattttccca atcgtttggc catgattgaa
    49921 tttcgtcaat aaaataaaat acgctagata aataagtaag gctgtgaatc cgtagagact
    49981 cagataagaa tctgaaaaat taagccaacc tagatttttg gcaataccaa gtgttaaacg
    50041 cgtaatagct ccgatacaca aaagatcgac caagaaggca aacattcgga taaaaaaacc
    50101 tgcatagaaa tacttaggaa aatcatttgc ttttggcggt tcatttcttg tgtccgtata
    50161 ctttttccat ttacgttgtt tctttttgat ttcttcgggc gtcaaaggct ctctttcaaa
    50221 agagctcagt accttttggg aaaagttgga gtcaggtcct acagcaggct gctgttgctt
    50281 atctttttct tgttccttat tttcatttga aagctctgac atgcttactc acctccgtaa
    50341 taatacattg cttttggtga ttccgctgtc cctagattct caagaacgga taagatctga
    50401 ctagtctcag aaggcttcaa tccctggaat tctgctagtt tacttcctaa ccatgtacta
    50461 gcaaacccag atggcgtgga agaatattct actagttccg catcttcaag ctgttgttcc
    50521 tttcgtatag cttttaaagc atcttcagga aatcccaatt catcgactaa acctacttct
    50581 tttgcttgga caccatcata aatccttcca tcagcaattt ttttgacttc ttcttcagat
    50641 ttattccgtc cctcgctgac gattgctaca aaacgactat atgcgctgtc gatatatgct
    50701 tgaagcaccg cttcttcttc ttttgtctct ggtcgcgttc ctgaacccat atctttcaac
    50761 gctccgcttt taactgttgt atcttccaca cccagttttt ctagaagtcc tgaataattc
    50821 aatcctgaca tgatcacacc aattgagccg gtcactgttt cttctgttgc aaagatttta
    50881 tctgcttgag cagaaatata gtagccaccg ctagctgcca tgtttttcat gctgacatac
    50941 attgggatat catgttcttt acgaatcgta tccaatttct tagcgatttc cgcactttcg
    51001 tagacaccgc caccaggaga atttacttct aaaaagatac ctttgactgt tggatcttct
    51061 tcaatcgcct tcagttgttc cataaaattt ttatgattgt aaccttcaga agcaaataaa
    51121 cctgaagacc ctcccgcagc aatcgttcca tctactgata attttacgat tttttgcgta
    51181 ctgtctccct tttccaacac atttggagct agttcttcat ccccatataa ccaactgttc
    51241 aatccctcaa tttgatcgtt ttcctgaggc ttagtcaaac tagctgaaac taacgaaacc
    51301 acaaataatc ctgcagcaat cagaactgct gcccaacgtc ttttattcat tctttctcct
    51361 ttctaataca agttttttat gacgatcaca gtttctgact gctcagaagt gtcgaaccgc
    51421 tctgcttttt cttcccgcgc atacccggaa aacgactctt ttgttaattc atcttctacc
    51481 gtaaactcac gagcttgagc aacttccaag gcatcaggtg cgatttcttg gatgctatcc
    51541 cagccagtat ctaaaaatat tttcgtatca tctgatacac cagctgatgc ttcgaatttg
    51601 gacaattgtt tttttaacat tcccagtgtc atgatctcaa tttttgaatc catttttact
    51661 cctcctctat ccgattactt tctattgtac cgaactcctg aaaaagatga tagataaaaa
    51721 cccattattt tttgacataa aaaggacatt tgacaaagaa tttgatatat accaatttat
    51781 tttttattct ttttttgttg acaagaacat tcgcaaaagg ttatgatttt aaatgtttcc
    51841 agataaaaaa caaaaaggag gatagttata ccttttagga tagcgccagg tctgtgtaaa
    51901 cagatgacga ggagagggtt tatcgaaaga ttcggcggat ggccctaggg actgcactct
    51961 acagaaaatc cacaaaaaat gtctgcaaag ataaaacaaa aggattttcc tgcagctgga
    52021 agcattcata cgaattcatt atagaaagta ggatgttttt tatgtgcgga attgttggaa
    52081 tgattggatt aaaaaatgta acaccaggat tgattgacgg tctagaaaaa ttagaatacc
    52141 gtggttatga ttcagctggt atctttgtct cagatggcac aactgattat ctggtcaaag
    52201 cacaaggcag aattcaaaat ttgaaaaaca aaataaatat tgatactaca ggaaatatag
    52261 gtattggtca tacacgatgg gcaactcacg ggcaaccttc tgaagaaaac gcccatccac
    52321 atacttccca aagtggacgc ttcgtgctcg ttcacaacgg cgtaatcgaa aactttgaag
    52381 aacttaaaat tgcttattta gcaaatgacc actttattgg tgagaccgat actgagatta
    52441 tcgctcatct aattgaaact ttcgcgaagg atactacaac gcaagaggcg tttttaaaag
    52501 ctttgcgggt catcaaaggt tcttacgctt tcgctttgat tgatcggaca gcacctgatg
    52561 ttatttacgt cgctaaaaat aaaagtccac tattaatcgg actcggcgat ggattcaatg
    52621 tgattgccag cgatgcaatg gctatgcttg ctcatacgaa ggaattcgtt gagattgaag
    52681 atgaagagat ggttaccgtc acatcagaaa aagttattat tcaaaatttt gcaggtgaca
    52741 tcgtagaacg ttcttcattc gaagcacaag tagatgcttc agatatcgag aaaggtacct
    52801 atccttttta tatgctgaaa gaaatcgacg aacagcctat tatcatgcgt cgtatcgctc
    52861 aaaaatacgt aacagaagac aatcgtgtct ctcttgatca aaagctggtc gatacattgt
    52921 ctgatagcga tcgaatctat atcgttgctt gcggaactag ttaccatgcg gggttagcag
    52981 gtaaacagac tttggaaaaa ttgacacaga ttcctgttga ggttcattta gccagtgaat
    53041 ttggctataa cacacctctt ctttcaaaaa aaccattctt tattttcttg agtcaaagtg
    53101 gcgaaacagc agacagccgc caagtgctgg taaaaatcaa ctgtctcggc tacccttcac
    53161 ttacgattac aaatgttgct ggttctactc tttcgcggga agcatccttt acactgctat
    53221 tgcatgctgg acccgaaatc gcagtagcat caacgaaagc ttatactgca caaatagctg
    53281 tactaacttt attagccaaa gctatcggtg ataaaaaaga atttgaaact tctcttgtat
    53341 tcgatatcac tcatgaattg agtttagttg ctaatgcgat ggaatcggtc atcgcccaaa
    53401 aagattattt agaagagttg gcagaagtat atctcagcga tacaagaaat gccttttaca
    53461 ttggacgtgg tgctgactac aacgtttctc ttgaagctgc tttgaaattg aaagaaatca
    53521 gttatatcca ggcagaaggt tttgccgctg gcgaattgaa acatggaacg attgctttga
    53581 tcgaagaagg aacaccagtg atcggaatca tcagtgaaga agtgactggt gcgcatactc
    53641 gaggaaacct gaaagaagtc gaaagccgtg gcgctaaaac actagttatt gtctcagagg
    53701 gattagaaaa ggaaagtgac cagctggtgc taccaactgt ccacccttat ctaactactc
    53761 taaccacagt cgtaccgact caattacttg catattacgc aacgctatta cggggattcg
    53821 acgttgacaa accacgtaat ttagcgaaat cagtgacagt agaataataa aagaacaagg
    53881 accagtgtca gacatctgac actggtcctt gttcttttat ttt
//
LOCUS       DACEOE010000003        51976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEOE010000003 DACEOE010000000
VERSION     DACEOE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.003"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1156..1554)
                     /locus_tag="IW774_000126"
     CDS             complement(1156..1554)
                     /locus_tag="IW774_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152507.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1770..2825
                     /locus_tag="IW774_000127"
     CDS             1770..2825
                     /locus_tag="IW774_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ1152508.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2845..3198
                     /locus_tag="IW774_000128"
     CDS             2845..3198
                     /locus_tag="IW774_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152509.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3380..3676)
                     /locus_tag="IW774_000129"
     CDS             complement(3380..3676)
                     /locus_tag="IW774_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152510.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3680..4498)
                     /locus_tag="IW774_000130"
     CDS             complement(3680..4498)
                     /locus_tag="IW774_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1152511.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYNLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4724..4945
                     /locus_tag="IW774_000131"
     CDS             4724..4945
                     /locus_tag="IW774_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAQ1152512.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4947..5213
                     /locus_tag="IW774_000132"
     CDS             4947..5213
                     /locus_tag="IW774_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1152513.1"
                     /translation="MSYQVRYEREAQKTLKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5326..5865
                     /locus_tag="IW774_000133"
     CDS             5326..5865
                     /locus_tag="IW774_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152514.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANKVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKKESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVS"
     gene            6071..6253
                     /locus_tag="IW774_000134"
     CDS             6071..6253
                     /locus_tag="IW774_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAQ1152515.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAETYTSKATVLAVSS
                     FLTLLTFFVTLSFSFV"
     gene            6357..6641
                     /locus_tag="IW774_000135"
     CDS             6357..6641
                     /locus_tag="IW774_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152516.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6648..6893
                     /locus_tag="IW774_000136"
     CDS             6648..6893
                     /locus_tag="IW774_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152517.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6915..7169
                     /locus_tag="IW774_000137"
     CDS             6915..7169
                     /locus_tag="IW774_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152518.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALAYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7251..7436
                     /locus_tag="IW774_000138"
     CDS             7251..7436
                     /locus_tag="IW774_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ1152519.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7503..7898
                     /locus_tag="IW774_000139"
     CDS             7503..7898
                     /locus_tag="IW774_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ1152520.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7888..8265
                     /locus_tag="IW774_000140"
     CDS             7888..8265
                     /locus_tag="IW774_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152521.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     ITFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8266..8661
                     /locus_tag="IW774_000141"
     CDS             8266..8661
                     /locus_tag="IW774_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152522.1"
                     /translation="MKQEKRKMKQTQTETINDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDCYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIDRNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8967..9365)
                     /locus_tag="IW774_000142"
     CDS             complement(8967..9365)
                     /locus_tag="IW774_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ1152523.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9556..10323)
                     /locus_tag="IW774_000143"
     CDS             complement(9556..10323)
                     /locus_tag="IW774_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1152524.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPNKLKKT
                     GEIVLPVKAIIKYMDVDQNLETELKNIVNTLQRQAVYEISNLDKEYKTYATVSAIDQI
                     HWDLEEQIITFNFSQEVIPLILSIKKKYPYFVFQDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(10359..11582)
                     /locus_tag="IW774_000144"
     CDS             complement(10359..11582)
                     /locus_tag="IW774_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ1152525.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKMFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLENHFTQYTFDQVVNLTSKYAISFFQLFTKNFNLYEANRDNLTWSTKSLDR
                     LKKPVFSIAELRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TDIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKFMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            12546..13349
                     /locus_tag="IW774_000145"
     CDS             12546..13349
                     /locus_tag="IW774_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1152526.1"
                     /translation="MSQEVTENVIDNDVDLVSDQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNVFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQQETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFESFDYEKIYAKKGRKVDRLRFTFSEPESNLPSISMHNWLEED"
     gene            13956..14411
                     /locus_tag="IW774_000146"
     CDS             13956..14411
                     /locus_tag="IW774_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152527.1"
                     /translation="MDAINWHEFVNYMEKLLDDLKSFSIADAVKHGLPVSTTMKMRGL
                     KVPDEIIKLRTKTIIETAKIVDSMNLDLKGKKTTYEKDFVRFLKNVIGKYLGKKSFTE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIVEYLENKEQKKGTKKGR"
     gene            14530..15588
                     /locus_tag="IW774_000147"
     CDS             14530..15588
                     /locus_tag="IW774_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ1152528.1"
                     /translation="MEKDVNSASTKDQYNIIGGLLGDKDKRTRHGLRIMLATFLGVFL
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLTFARSGAEITISDVWTDKTRDVTVVKLG
                     YSGNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDYTADDDTKVNVNESDSVTKALSQYSMNDVDNEGVISFGKKAKPVN
                     TPDNINFRINPYSDTTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKTINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15630..17579
                     /locus_tag="IW774_000148"
     CDS             15630..17579
                     /locus_tag="IW774_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1152529.1"
                     /translation="MENSTEFFKNIQTEFNHALTLMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAKRQRQEGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TIIPRLKEYERILKSEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPEASLK
                     LSKKIPDELLEEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDIAKLEEELKSIKGQPVAVYTVTMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRKLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVNDTVSLSNAILFATTNAGAE
                     MYDSMNRYSSLSRVANQENSNEYSTELKREYRKVALEIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAHNEILKSAELVRKHLNVDLRLPPQKKHFYGQEFYADDLSMHLV
                     FDRASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSKAGGFEGKGDT
                     DAEIIAKPSIAESKENKFVFSVGGV"
     gene            17582..21271
                     /locus_tag="IW774_000149"
     CDS             17582..21271
                     /locus_tag="IW774_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152530.1"
                     /translation="MKFKKSLLQNRKIKRWVVRGLVIVGALAVTLGAPFGMNDPTSDD
                     QGNKQETVANTNVAYAESKPAEKGVWTLYARILMGDKEVNDSATKTGQMNLLGQGGIS
                     ADFPYSDISKQGNAIAGGNAGTQLASTLATYNYYGYIETVSGNAIASQASSFIGGIGR
                     TLGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGKGGSDVSNPVAKALTSLL
                     NNLGLNNQLFKAIASLSMLFFTAILGYQGMKLLSKEGVNSKGLSPVLEKWIVRIFVLF
                     VFLPVAGSLLGVYINGIQSAKEDGKLPTNVVGDYIINTRLWASTQNLAPSSDTGGNVP
                     NGKPTSEIKFVDTDYEPSKKSEMIRMINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NVNTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGNAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTTNNFDATQPWGVANNATFSTQSVALMLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFVRVWEVVLTGSLAAGAGVMAISLGVVTTSLIGLQLPIFLITFVRAFGDTAV
                     GFIPNTLLGDGVANIVRSAMVLGVGYLLVFKKGEKVNNNLINMLIGLPLEVAFGFDER
                     AKLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGRGSVKDMVAGGLGAVGAQGIAD
                     RLNNMGDSNADTKTIADKVVPETPASVPFNGGGVGGTINNTSFKDLVRQDQLRNNSQQ
                     YSDHLGRMVDEKGNFVDENGEVLKDSKFGKDAKGNFIDDKGRLVDEDGNLIDTNGKPL
                     EDNEDGNIGMDSQGRLIDDKGRLVDDEGNLVDENGRKLLNGDFGQDVNGRLVDDKGRL
                     VDEDGNLVDEFGKKLGKHSNGYNVNGDTVDEDGNIIDSRDALVADRNKELEQQQHTPI
                     AVAIPDDESDEVILDQAHQGLREEAKDKVVSDQAQEELKNKSNGEDVSNQYHQTAGNV
                     PVEGQSNELHNNFDNVADRNDTVLKEKESLRQETDKDQSKLKKTTIPTPIGSTVPKVN
                     QANDSETNTGLGMAKDNQSIREPKKTTESSKSNKSKSTSNKTGKSKEPSKAKSAVEYL
                     SKTGKNVYTSADKALAKAPKGSLAGMTYEAKNEAIKLGKGTAKVAKVTAKGATKTGKA
                     VKKSTMSVKDYIAKYSEQKPTDKKS"
     gene            21683..22021
                     /locus_tag="IW774_000150"
     CDS             21683..22021
                     /locus_tag="IW774_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152531.1"
                     /translation="MFDSIKNLVSGMTPEMKTLVQAILYLMATFFALKPGFTAMKHFG
                     DAKWGEGLKYVGAVVAIFIIPVITTVMLLTMGTKTGNDLNQKANMISALIPVVGIYLV
                     TKFPKEEQLG"
     gene            22133..22369
                     /locus_tag="IW774_000151"
     CDS             22133..22369
                     /locus_tag="IW774_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152532.1"
                     /translation="MDREKMLVHANEVLTSGFKGTDLAKIMNMNVNQFYDYRNGNKKI
                     EKARLETLIKFEKAYLYVLDKQKRTIDRKKGVLQ"
     gene            22369..24534
                     /locus_tag="IW774_000152"
     CDS             22369..24534
                     /locus_tag="IW774_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152533.1"
                     /translation="MAKFKDITESINYVAETVPVVVLSLKPQLLEVYQKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYVVIEDVQKLSSEEFPDEEDAILDSEKYVVLLKSSLN
                     YVVDLPEKFKEKLLAGVRFMDSNGQLLVIPDDPNMFEREVEEATDFYSAVDQLEVATK
                     GLERIAETFNSSDAVEFEAVAEAETEAVPDEEEALFEDNDEATEAGNQEASIEETQTE
                     TYQQPKIDEKPESQSDKENYVYEAEKIESESDVPKNLDADLLEKDFEQIIANKFQPVV
                     LDEVSDPVDLNEEMRSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLLLKGK
                     QMLADELLRIEEATSLSGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLEAELKKLEQRYDAEHLPEVKQQEDEFRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIEKSLTEVQEKTLEIVEKTKAENEAEFHDTKEKTTDVVVK
                     YFANKEEVEKLVAKDTNELESKLVAQQNIIEDYKNRVKEFKDLADGYKLNSQKLTEQS
                     EALREDLSRARNDLSQMAEKTKSLVVTTENQTESKRDFTSKKMATIIAVSAMACGTIL
                     GSSYFISHNHQEAQAQTETSISRVENSLGANTASTSTTDNNYEATPTTEFKVGEDVVI
                     SIDGKTTKAEVLSIDGDKAVVKAPDGNQYKIQAHLTEKN"
     gene            24546..24776
                     /locus_tag="IW774_000153"
     CDS             24546..24776
                     /locus_tag="IW774_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1152534.1"
                     /translation="MREENIVYPVIENQANTKSSQLLQEVQVVYPVNELPQTNETVSK
                     SISLIGLLIVATALTVGVYRKLKVKGSISDGR"
     gene            24766..25632
                     /locus_tag="IW774_000154"
     CDS             24766..25632
                     /locus_tag="IW774_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152535.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVDWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLRFIFIPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNTARVTMGKKKPFRGTEILKVRQDGTIMYRDGSVALMFNLDGATSLTAFP
                     EEIIAQEGKSIQYQNARDRSLAEIKITSSQPQNTERQIRNMKVQKRNAKSNKAVFDIV
                     DQQERFVSEYIDGVKTTVIQYLILKSQSQMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSEIYALK"
     gene            25650..27608
                     /locus_tag="IW774_000155"
     CDS             25650..27608
                     /locus_tag="IW774_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152536.1"
                     /translation="MELMSRSKLLNFRKKKHKKEQNADSSKAVNNTYVSDLTSLVGYQ
                     FTPSYIRTGNRWGTIVKLVNTYGMNRNYEFGWMVKAIPEVKVEGVKAYLIEADKPVSQ
                     KDQQEIMHENVGGSTNSYENNLNDKNSDAEINKTVMRIIDLNTASLLDSTANKIIDWK
                     LEILLVSDSPDKISEQLIKLQRLYDEKMYGLKLMSFGGDQRDLFDRLLSEPVGSVYEY
                     TSMSSDFAGNDHAIRKGLDDADGWAIGTMSTSYASGVAFMAMDRSFKGKVLVASHKDA
                     TIKGYSEEISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGETFVAKPSLNSV
                     IEKINLAEEGLNSLELFGDRKDIGDLYRRNLDKLTQIFYLLADRELNPATRVLLKKEL
                     GNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEIDRII
                     VLKDTLRTALDSFSKMFNQRTTLKEIGKSDKLQIYYNLSDLRTEINVMEAQFLNTFDF
                     ITSQAKKGDIVMIHGFDRLSVETVGLIKKRLTMLEEDGVRLAFLFDTIGSGEDKDGIE
                     TADIFNTDGKIYQSFESDFNYTIIGKMTSEELMKYEKKVAPARKGEDDQKLSVDLRQY
                     LTSGRSNKFQIRRPVDLTNNFVIGEFII"
     gene            27790..28284
                     /locus_tag="IW774_000156"
     CDS             27790..28284
                     /locus_tag="IW774_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152537.1"
                     /translation="MNNLPEKNRVFRLKGDLSQFKLDENGEFFVWMRHEHCWCNPFEL
                     RKENEIRTKIENGEWDYLFRWSDESFIANNMDHAFVSFLDNTWGTWYDCYLKKLGFQN
                     IDIDAIECLKEVDNKYEEEPYYLLQQKLANDSAFQVEVWENEDGTYEGLAQEIPLSEK
                     KCSI"
     gene            28272..29381
                     /locus_tag="IW774_000157"
     CDS             28272..29381
                     /locus_tag="IW774_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1152538.1"
                     /translation="MFDLKKVYQHFLCISYITLFSFIFLFGVSVIVGTIFIWLTDEDM
                     SLKWFMCLLSCIMLVLLCLEVREFLPSHWQEKRKELREEIEIKTSDWMDHTWLPLKKN
                     IKQKEFWVLQLWALGKFCSYVALLIFDLIFYSIGIFGTAYYLLTDHNRLAVSDIFTVL
                     MTLIFVIQLSIFSQLLIRFIRQKITIDKLFRSLVDLLIISVILMTLTERIEQSGKTVE
                     GLKEFLTMFIQFVNGHMYLFVSGIILSMIGYVTTKLIKRSIEKEIEIDLIPTLSGYDY
                     YVNYGKFEELLHTNIVRTSIREFTNKEIKDSIHGEPTRKFIDRQKKVYNFYEVEAILR
                     YGIGKLWLEGRNIRQNKPYVFWKSGVLEQFPRRNR"
     gene            29402..29737
                     /locus_tag="IW774_000158"
     CDS             29402..29737
                     /locus_tag="IW774_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152539.1"
                     /translation="MKQYLNKWKVIEGSLVAQRIKGLPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYEGQLTPFYTTWEMSEDGINNFPCESI
                     EESISQTEC"
     gene            29898..30086
                     /locus_tag="IW774_000159"
     CDS             29898..30086
                     /locus_tag="IW774_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152540.1"
                     /translation="MTSEELMKYEKRVAPARKAEDEQKVSVDLRQNLTSGQSNKFQVR
                     RPVYLTTNFVIDPQTFGC"
     gene            complement(30690..32285)
                     /locus_tag="IW774_000160"
     CDS             complement(30690..32285)
                     /locus_tag="IW774_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152541.1"
                     /translation="MFILDDEINKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALV
                     LDLIDKLSDLANIYEDDTSKKILEEIAKLRKKDPNSPRWEMELLEKTYNSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGDTWELREITKETCQDVIRKSYEIVFLKNPILGKKV
                     TNEIIQYASRIHSMSVTPEEFHSALKNSYLKKLSEKAKENLCKTAFKFVFKLPYGNEE
                     VDRDRVSTFRLLDALIFDSKEFYLKILKKEISNYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIASHPELQRDFSEEIIQNLKISIKDFEPKQPITTHIEDYYRLRSLAVLGNT
                     KKELQEHLNRLLDPILQQNMRVSSLATSTLETIYNQYLYFGEKEMFLDFMIEHLTDSP
                     AFKFSDKDMEVLPWIYDKLNQDKFNKLLYNLNRNNQFYHNGYFSSFISNLLDFYKQKF
                     HMDLRTHYNGLLYPNAVTMDQKFQLKDKELKKLYELAESKSDTYPEILNDLKLNLITK
                     SEQYLGEKINSEEKLLDYIKQIK"
     gene            32407..32991
                     /locus_tag="IW774_000161"
     CDS             32407..32991
                     /locus_tag="IW774_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152542.1"
                     /translation="MGLKQSVYIWLYPDNPMSIMADIFVGSILLCLLYLIFFHNLIGY
                     MILDWKGMFDIERKPFNNKQTYTTFAGGDLEEKEVDVTHEIGLALSGIQDAVALGSMI
                     TDTGYNAYEVFYLMYFIWRDSQDIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            33002..33253
                     /locus_tag="IW774_000162"
     CDS             33002..33253
                     /locus_tag="IW774_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152543.1"
                     /translation="MKKIIDQPTNRLIVEGVIFVTMYFTVGNGISNTIIQKDPQYLLF
                     SLYAWIVQMLWVSLLMLLFYWIVRVILLVLNKAKKKKDR"
     gene            33260..33982
                     /locus_tag="IW774_000163"
     CDS             33260..33982
                     /locus_tag="IW774_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152544.1"
                     /translation="MKATKLKLVLFSTAVLLTLSACGTSKSADNTKDTKSSSTVVTES
                     SSAQTSTTMSSMTENNAEKFQKYKDVNQGKKPEEVYNEFISSVKNDESYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIQEGFSYAPTLMHDITIGKPAYEYHAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTLAVVTPKKIDGTLYWDLMGMTTNTSDEMIESTSNYYM
                     DLGAYKSYQQSEPVMDDQSTIK"
     gene            34112..34855
                     /locus_tag="IW774_000164"
     CDS             34112..34855
                     /locus_tag="IW774_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152545.1"
                     /translation="MKEISASEAHISQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQELVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            34861..35226
                     /locus_tag="IW774_000165"
     CDS             34861..35226
                     /locus_tag="IW774_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152546.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            35242..35772
                     /locus_tag="IW774_000166"
     CDS             35242..35772
                     /locus_tag="IW774_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152547.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            35934..36323
                     /locus_tag="IW774_000167"
     CDS             35934..36323
                     /locus_tag="IW774_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152548.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     RKLAQSYLEEKAGAIKDKEEMEKFAIE"
     gene            36316..36705
                     /locus_tag="IW774_000168"
     CDS             36316..36705
                     /locus_tag="IW774_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152549.1"
                     /translation="MNEKTELFLKKVNFLVEKYFLTKHKIMYLIKGLIGGIILWILTF
                     FSLFGGFTNYKKLVDENVKQISASESLRHQAEDLVSNPLFIGGMVEVIIISFVIGYLG
                     MCIFQIIFDLIRGKVFQGFKFEKINLR"
     gene            36735..36959
                     /locus_tag="IW774_000169"
     CDS             36735..36959
                     /locus_tag="IW774_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152550.1"
                     /translation="MEQTNHITEETRKFICLESFYSEGRYCNKGETYTAYPIEGGFKL
                     VFENGDMNFTTELFECVVETWSDVLLEVTK"
     gene            37012..37272
                     /locus_tag="IW774_000170"
     CDS             37012..37272
                     /locus_tag="IW774_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152551.1"
                     /translation="MAYENLQLVVAGVSGDIYLAKAKDGIMDTNYRRVATDDVLSSAT
                     EWFRTNEESAVHFKGIDGNIHSLFYTKDKEKAKKILDILKES"
     gene            37611..39851
                     /locus_tag="IW774_000171"
     CDS             37611..39851
                     /locus_tag="IW774_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152552.1"
                     /translation="MSKYEFLDINVGSAFLINLDMFEAWAQSSGYNWAFSDDMIEFLS
                     RTMSWESYKVKQFLDVIAKPIYTSADDHWSGRELATLKELAEYNKNYNIPFQVSYTTD
                     DFSVIYALGKESRYLIPNRYLEIKMNSDYTNYTLGELKALRQSDSEEFSTLKNEVSTE
                     NFRISDIEELKKQQKQQLEKQKKLIEDIAYKRVDELAVQEAKIEKMRQELYEKQQSML
                     AEANKMRFELEKKVEELNTKIFYLETEIDAIEGYFGESTTFVQLRSGQKSLPEDPVVM
                     FQKVRYLDEELGRYFSMREVEGTADTMLTFEQALSSRDDLFDLFSPNKKSISMIRISK
                     SGYGYTNGQMESTNMLEKYKKYHGETLAILLRNGDNLWICWLDENKISFKDDLFYQNK
                     VTEQASETKNMVKEIDWTHTRNLEERLKDAKKQKRAVRGEFASRYILFSILQGVLDNG
                     TMMTLPEKVNVIQAINQKSPYFIASVADGWLEDTRLPSFSELIDKYSFYKETGEDRVL
                     EAEYELNPDKFENMRDYPHKQNDDIYVLYRLRGWSPTSANYTYYNSDNRSNSESGRDL
                     AYSIDLKDGIYQINLVKQGKFQKQMFSRTFVGVDSTTGENIYDYYYERETEYYIRGQK
                     AYNKSDYWGDKAKRLTANFRVYESEFINLTFLNSVFIDYVITTKRIGSYSSTYASLVP
                     MLNSMSRFLKEREEKELQYIKEIMPDFEMHEWQIALSDWKLTNKVRRITPFQAKRFLK
                     AMKFVQ"
     gene            39989..40786
                     /locus_tag="IW774_000172"
     CDS             39989..40786
                     /locus_tag="IW774_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152553.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKKIQKLEKKVHAWKYKDSMSGYGESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSKEEVRELVETPVRKKIETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYLQYNSNRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IKLPTGQISLGEKDYLIKVGKELIIKVPERLFKENFEIRETLVWKEE"
     gene            40826..41008
                     /locus_tag="IW774_000173"
     CDS             40826..41008
                     /locus_tag="IW774_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152554.1"
                     /translation="MAVNRKLQVLITDDFGGAEWFDVQSIDFEEEVVVIENVYGEEYS
                     YSLEGENVEDLRLIGE"
     gene            41052..41339
                     /locus_tag="IW774_000174"
     CDS             41052..41339
                     /locus_tag="IW774_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152555.1"
                     /translation="MINKKTGKRIFSSKDEVIRDLSENFEPIELFFHQLNLEIEKLLK
                     EPTEGRINKVLKLIACKDSHAYMLYPKWYADKNIAEINDYLASIKERNAIN"
     gene            41376..41663
                     /locus_tag="IW774_000175"
     CDS             41376..41663
                     /locus_tag="IW774_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152556.1"
                     /translation="MAEVKFGSKEWFEELNSMIQFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            41754..41993
                     /locus_tag="IW774_000176"
     CDS             41754..41993
                     /locus_tag="IW774_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152557.1"
                     /translation="MKELEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHFPKVRFYN"
     gene            42121..42450
                     /locus_tag="IW774_000177"
     CDS             42121..42450
                     /locus_tag="IW774_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152558.1"
                     /translation="MKLEGSLQNRMMEGRTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELIKTKNGWKVLKENTHFYIGDRE
                     KYYDPSF"
     gene            42503..43351
                     /locus_tag="IW774_000178"
     CDS             42503..43351
                     /locus_tag="IW774_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152559.1"
                     /translation="MFSQEEIISKSQPDKNGTITMKKKIPCYRCSGSGQYKFMSMGKI
                     TYGTCFKCDGDGFLVVTEKSFTPEYRAKLNALNKKRADRKIRITREKESQKNFDRYGF
                     EKGYIYVVIEENSYAIKDELHHQGATYDPLLGWFFKEKQEQYKTQKVFVEEFSVINDE
                     GIIELKLEEYSERQQEILRAKRREEGEFIGTIAQRLELDLKLTNTVTIDTQWGNVYLH
                     LFEDQNHNSLIWKTNKFLYYDEWDVGEIRKVKATIKAHDYYRDTKQTWVKNVKIQKNE
                     SEGIKV"
     gene            43348..43665
                     /locus_tag="IW774_000179"
     CDS             43348..43665
                     /locus_tag="IW774_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152560.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            43668..43901
                     /locus_tag="IW774_000180"
     CDS             43668..43901
                     /locus_tag="IW774_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152561.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIFQRFRLPVSE
                     LKKMMRLSAYLLMFLNVVTLIFVVIGVTFNLME"
     gene            complement(44017..44496)
                     /locus_tag="IW774_000181"
     CDS             complement(44017..44496)
                     /locus_tag="IW774_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152562.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            44617..45954
                     /locus_tag="IW774_000182"
     CDS             44617..45954
                     /locus_tag="IW774_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1152563.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            45977..46504
                     /locus_tag="IW774_000183"
     CDS             45977..46504
                     /locus_tag="IW774_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152564.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            46491..47054
                     /locus_tag="IW774_000184"
     CDS             46491..47054
                     /locus_tag="IW774_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152565.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            47476..48213
                     /locus_tag="IW774_000185"
     CDS             47476..48213
                     /locus_tag="IW774_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152566.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(48230..48646)
                     /locus_tag="IW774_000186"
     CDS             complement(48230..48646)
                     /locus_tag="IW774_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152567.1"
                     /translation="MNKKRKLSVLITLISILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            49039..50286
                     /locus_tag="IW774_000187"
     CDS             49039..50286
                     /locus_tag="IW774_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152568.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            50556..>51976
                     /locus_tag="IW774_000188"
     CDS             50556..>51976
                     /locus_tag="IW774_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ1152569.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKIKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEG"
BASE COUNT        18769 a         7568 c         9852 g        15787 t
ORIGIN      
        1 acttttgttt tgtgttaagt ttctttatat tactacgccc attatgtgta aaatttcttt
       61 attcatccca cgctccatgt tattttatat ttcctttttc atgttatttt tcgtcagaaa
      121 ccgatctagt ttttacctat tcccaaaatc catttattcg ttatttttta ccctgattaa
      181 ggataattat ttgatgctta aattagctaa aaaagcgatt tattgccgat ataatcatga
      241 tattaaagct gaattaggcg gaaaggcaaa atgttcgcaa aatcgaagtg tcaattatct
      301 tgttttttta taaattttta agtgaaaata tgaaaaattc tcttttaatt ggtataggta
      361 ttaaaattac ttttaatcac tcataaaagt atgcgctttc atcgtttttt atgagaataa
      421 tatcaataaa taacgatata aaaaccattt ttgatactgt tttataagga tataaattga
      481 tctaatttag aaaaataatt caaaatttaa aggttttaat attacaaata gaataatgtt
      541 atcgtaaaat ggtataaaat agctgaatat tttatttttg ttattttatt agcagaaaca
      601 tctaaataat tatacatgaa tccatagaaa tatattggtt tcaccttcag attaatattg
      661 gatcatatta atctgaatat aatatatgaa ctaataaata gcttaacctc gctattttgt
      721 tttttctgat cactacctac acagcattag taaatagaat ttaaaaaata aaaaaattaa
      781 gacaaaaaaa gtatcaacat aaacatatac atatacacgt ttatgttgat acacttaatt
      841 tgtcctttaa aaagcattaa ttattgaatt atgttatttt ataatttgat tacaagctga
      901 ttgttaggtg ttgtccttga attacatttt tcaacggtta ttttatcgtt taatttatta
      961 attatttttg atctaaaaat taccttaaca aatatataat taaccaaccg ccagataaaa
     1021 atgcgatcat tgctaataaa taagcaaaga taaaaatatg ttcgatcagc ttaaaccctg
     1081 atgcgattaa gcagaccaag taaaatagaa attctcttac agataatggt aattttctaa
     1141 ctagcatact cataattaac accccaaagt aatattgtat tttgaacatt ttacatccaa
     1201 tacagcatca atcaaccatt gccaatcaaa aatgcaaggc ttcattgaag tatgtttttc
     1261 agatgaaaag atataatata atttcttcgg aataatattt tcagttgttg ggatcaagct
     1321 aaatttaata ccatctaact catagctacc agctttctct ccattaaaaa tttccttagc
     1381 actttcttct aaagaatcaa aatatttttc gacagcaacc tttgaagctt ttactattga
     1441 agcatttccc ttttcgataa aatctgcttt tgttttaccg acaataagta attcttgatc
     1501 agtaaaatct tctaaaataa tagtagtgta attcttattt cttctcaatc gcatcaggat
     1561 cactccttaa tataatttat gtttttataa tatcataata tgataaattt gcaagtgttt
     1621 atttacacct ttttacttac actttattgt aattgattat acttttttga ttgcatttct
     1681 ttcttataaa cagttgcaac tattagtaaa taggtgtaaa ctattttcgt accgcaatac
     1741 cataaacaca aacagaaaga ggtcatacca tgaaagaacc attattgttt tccgttgact
     1801 tagggaataa atcagtaaaa gaaatgagta cacacgtttc tccactctcg cttccctctc
     1861 gtattttgaa caaaaatatc attaattcag gacgtttttc taatataggt tcctcattaa
     1921 aacgatcact aaacgtttct gaatatagcc tagtaaacac tactgaaagc tattattttg
     1981 ggaaggatat acacgagcta ggaaaagatg actacgtgat agaatcacta ggttttggga
     2041 tcagcagata caagcaggaa acctaccaaa atatcctatc tttctcttta gctaacctag
     2101 cacgtttaga agatcaatct gatgacgttt taaccgttaa tctagtttta ggtctgcctt
     2161 catccgacta taccgaaaca atcgtcaaat acgtgatgca acaagccaca cgacaacatt
     2221 cagttgaagt tgacggtaaa gcttataata taaacgtaga taccgtttgg gtactgccac
     2281 agccgattgg aacactatat aacctcctcc ttgctgatga tggtagtgta caagatgaat
     2341 cattgatcag tcagaagatc ggttttgttg atattggtgg tggcacgaaa ctttttgatg
     2401 ttgtccaaaa ttttgaaatt gacgaaaaca accgtgaaca aaaggatacc ggtattttta
     2461 ctttatataa tcgtatcgta aatatgcttg accttccgat cagtcctaat gctttccaac
     2521 ttgaaaagat tatccgagaa ggcaaagaaa aaggcgtata tctatttaag ccaagcggaa
     2581 atgaaagttt tgatatttca gaagtagttg aaagagaaat aaaaacatac accaatatgg
     2641 ttgttgcaga agttttagct acattcaaaa acttcacaac cttagataaa gttgtcttta
     2701 ctggtggtgg ggcttctatt atcgacaaac aacttgtaaa agataaaatt ccgcatacct
     2761 tgtttgttga agacagtgaa ttttctaatg taaaaggctt ttacaagttt ggtttaactc
     2821 aataaaacag cgaaggagat caacatgaat aagggaaaat cagagcgtta tgtagttata
     2881 atcaatagag aaaaggttag cgagttacct ttagcagagt ttcttgaatc tgaatatgaa
     2941 acactaggcg ttaaatactc tcagacgatc aaaaaagctt tgaatctata tcggcgtgta
     3001 aagaacaatg aagttacaat tattgaagca ggcaacgata gaaacgatag caaagtagca
     3061 gaagtaaaga ataaaccaac tgatcataac cctattcaac aagagcatcc ttcaacggtt
     3121 acatccgatc attcagcaaa gaaagaaaag ccaatgctaa aagggttcaa tgcaccaagt
     3181 aacaaagact tactttaaaa ataaaaaggc gaggttacaa tacctagcct ttttattatg
     3241 taaaaataca aacataaaca tatacgtata catgtatata aacatgtata cgtatatatc
     3301 cataaacacg tatacgaaat ttagttaagc tatacctaac cgcttattcc aataaaaaaa
     3361 gtgatcagct ttaaactgat cactcaccaa aaacttcatc atacaagtta tctatcattt
     3421 caccaagtaa agctgaatct gatcgaattc ctaacttttc gctgatttta gatagtttct
     3481 ttctttgtga tggttgaagc gtgaattgat aatttttctt cttttcagaa ctatcacttt
     3541 ttagtttagc taccaaagaa cttgttgttt ctttaggtgt ttgtttaggt tcttcaactg
     3601 cttcaaccac ttcaggaact tcttctactt cagcttcagc gaatttttta aatctatttt
     3661 ctttcacttc aatcactcct tattcttgtg ccgtatcaat tattttgttg tatagcgtaa
     3721 acgtgctttc aaagaaatcc ttattcttac gatagatatt cttatcctta gcgaactcag
     3781 caatattctt accttctaag ctagttgcat taagcaattc cttctcttgt acatatccaa
     3841 gataattaga taacgttggt accaattcta ggaactcacg acttgaatta gtattatgtt
     3901 tgatcctgct accgatcgta gcgtatttag tttccatcag tgtttcacca gtcttaatat
     3961 caatcaactg ttttgctagt gcatcaatat ctttaccaag cttcagtaaa gcttcaaaac
     4021 catctttaga tggatcagaa atgcctacga caagatcaga tacagcccaa gcattttgag
     4081 ctagtagacc tgcatcatta tgagtatcaa tcagaattag attatacttc tctttaagct
     4141 gttcatagtt aacaacgatc catctaaaca gtagggttct gttgttaggc atatcacgca
     4201 tttgcatctc aacttgtttt aaatcttcac ctgctactaa aatatccatg ttctcattta
     4261 tagctaaagg ttcagctgat ccatgaagat ataaatgatt gattgtattc tctcttttcg
     4321 ctttactgaa agcatcagga aagcgttttg ataggttttg cgacttatct tcatcaatat
     4381 acagcacctt catacctaaa ttttcgacag catatccacc tagattgtag attgttgttg
     4441 ttttacctgt tccacctttt tcaatgtttc cagtaatgat tgacaacttc ttttccatga
     4501 ttaaacctcc cttattaaac ttttgtaact ccattataca ctataaacac gtttatgtat
     4561 atgtttatat acataaacgt gtttatattt atatttgctt ttatattcta caacataaac
     4621 atatatatgt atatgtttat gtttatgtat atgttcatat atatagctat tgcatctgtc
     4681 atacaatcgt agtacaatgt aattgaaaag gagttgataa actatgccag taataagtat
     4741 tcgtgtatct aaagaagaaa aagattttct tgaaaaagtt gctgaattca acggagataa
     4801 catttctgaa tttatgcgta gtaattccct gcaaacagca gaaacgttag tagactttgc
     4861 cacctacaag gaactaatga aagagcatga ggaaaacgat caaagtattt cccacgaaga
     4921 tatgctaaaa gagttaggct tataagatga gttaccaagt aagatatgaa cgtgaagctc
     4981 aaaaaacgct aaaaaaaatg gataagtttc aggcaaaggt tatcctaaac tggatcgaga
     5041 aaaatcttgt aggcactgat gatccaagaa agcatggtaa aggtctaaca gctaataaat
     5101 caggctactg gcgatatagg gtaggtgcat accgtataat agctgatatt agcgagcatg
     5161 aagtgacaat tcttatattg tctattggtc acagaagtga agtttataag taacagacaa
     5221 gctgataagg cttgtctgtt tttttgttaa cccaacaaaa tcactgttta cgatcccact
     5281 ttacctaaaa aagaacaata tcctcttgtt attatacaca ccagtgtgta taatataagt
     5341 gtaaacaagc taattaactt tcaaaaggag cgatttttta tgggatggta tggtgcttat
     5401 tcatcagcaa ataaagtaat cgaagaaaag aaaggatacg ccaaaaatgg tttggaaatc
     5461 gtagatacca aagtaacgaa gacctatgga attatccttt tcaaagatgc agaaggaacc
     5521 tacgatattg acagcttcat ctttcgagat gaaatgtaca aaccaatcta cttcttaaat
     5581 gacagtcctg cttactttaa gaaaattcca aaaaaatggt tgaaattctt tgtcggtgat
     5641 caagaaaaaa tggttaagcg acaactagat agaattaaaa aagaaagcaa taaaccaaaa
     5701 cttgatgact tattagttgc aggtaaaact tactcaatct gggatggatc gtatgtcgct
     5761 gtttatcagt ataaactcaa acgtagcttt atttttcgac tacaagatgg atcactcact
     5821 cggttcactc gactatcagc aggagatgtt aaggaagtat cctaaaactt aaatacataa
     5881 acataaacgt gtatacgttt atgtttatgt atatgtatat gaatataaac gtattaggta
     5941 cgaacgcttt taaatgcttt tctgggattt gattttaggg tagggctaaa aacatcacca
     6001 tctgatccaa aacagctcag aaacgcattt aaacgtatct gataccaata ttacagaagg
     6061 agtgtatcgt atgattaacc tatttctttt agtgacagct agtacattat tctttactgg
     6121 aatatcactg ataatttcct atttgatcta taagaaagcc gaaacttaca caagcaaagc
     6181 aaccgtacta gctgtttcat cctttttaac tttgctgact ttttttgtta ctctatcatt
     6241 ttcttttgtt tagcacagct gatccatcag caaaaaacct ctctttacat tatacacacc
     6301 aatgtgtata atgtaattgt aaacaagcta attaactttt aggaaggagt gacaatatgc
     6361 taacagctac attacagcaa cctactcacg ttgctattgc agaaagctat acaattaatg
     6421 caggggtcgt taaacagctg atcaaggatc gagaattcaa aaagtttaac agtcgtcaag
     6481 cgtataacag acagttaaag aaagctattc taaagcacaa aaattccttc atcgggaaat
     6541 tggaaagtgt ggatgttaaa actgtttcaa cggcattttc agttgttgaa gtgactattg
     6601 acggtcgctt gatttctcta tttgcttttg gaattaattg atagggagtg tggactttgg
     6661 aaggtaagac ttttgcagaa atttggactg aatttattga ggtaccaact cgcattgatc
     6721 cgataacttc tgaaaaggta ttggatgcag acttctacaa tctcaaagca ggtactagcc
     6781 ttgaatgcgt ttggatgtat ttagaagagc atttcaaagg cgtaacgctc ggtcacgtct
     6841 tataccacaa tggtgcggag tgggatgcaa ttggaaatat ccatttttct taatacaaga
     6901 aaggagaaca caatatgaat tataccgatg aagaacaaat cactttggaa tacgttttca
     6961 caaatgaaaa aggggaggaa attgaaaaag tcttgatcat ttcatcaaaa gacttggaga
     7021 acgctttagc ttatgaacca tacgaatcaa ccgtaactgt caatgaattc ttagatacct
     7081 atacttctaa tgatacttgg gaaattatga atatttgcaa tgctaataac attcaatttg
     7141 ctgaaaaaac caataaatca ttttattagt tttgcaataa aacatcctct ttttctattg
     7201 attccataca caacaatgtg tataataagt tttgtaaagg gagggtagct atgcctaaga
     7261 cagcaagaga aattgtaaaa attctcaaat ctaacggctt caaaaagact ggacaaactg
     7321 gcagtcatgc aaaattttat aattccaaga ctaaaagaac ggtcgttgtt ccgatccaca
     7381 atggtgatgt accactagga acagaagcta atatctggaa gcaagcaggg ctttaacagc
     7441 cttgccttcc tatttggtat taggcatact atcctctctt acactagaaa aggagcgagg
     7501 aaatgaagac aaaaaatatt ttttatccgg caatcttcga gaaagaagga gaagcttata
     7561 acatcgtttt ccctgactta ccggaaatat ctacctttgg aaatagtctt gaagaagcat
     7621 atttaaacgc taaggatgct ttaggattag cgctatatag tgtaccagat gcagaatttc
     7681 ctaaaccatc agcagtggaa gaattaactt tgaaagccaa tcaagtggta gtaatcatcc
     7741 aactaaacat taaacttttc cgaagaagct taaatacaaa aacaattaga aaaaatgtta
     7801 gtgttccgga atggttagtt cttttaggga aggagaaaaa cataaacttc tctcaactac
     7861 ttcaaaaagc actagaagaa gagttgcttg aaaagtaaaa aggagaccga acctatgaaa
     7921 gctacacact taaactattt ctctcgaacg ttaatcaata atctattaaa agatacaact
     7981 ggcattagcg ttagtaccaa acactttgcc aatgatctaa ttacttttga ggataataac
     8041 ttgcgaattg agatgtttat tgaacaggca acccctaact caatacctga attctgctat
     8101 acgcacactg aaaaaatgaa acatgcaaaa tcagttaaag atgttgaagc attaagtgaa
     8161 tcactaacga acgctttaaa cctcgttaag aaggcaaaat tgttatttga atttcaaacc
     8221 gactaccaca atgagttgct tgctcaatct gaaattttag attaattgaa acaggagaag
     8281 agaaagatga aacaaacaca aacagaaacc attaacgata cgcttgctga tcaattagca
     8341 ggatattata aattatttga gatgagtaaa gcacagcgct tgatccacta tctttggaaa
     8401 tacaaaattt tgtttctttt ttgggaagca acactaacat tctcagttct tttttataag
     8461 tcagattgtt ataatcaaca cttagtttta gggagttttt tgcttatcta caccattttg
     8521 atgcttaatt tatataccta catcacactt gaagaacact tagtaattag cgatgcaggg
     8581 atcgatagaa acatcaaaag ggtatctttc aagatcacgt taaacaagcc taccgataaa
     8641 aagctctcag aagcgttctg agagcttttg acattttctt gtataattta tccaaacaca
     8701 ttacaaagca cctaattcga gcttatatca agcaaatttg ctatttattc ctcaatacat
     8761 atctaaaaat tacttgatgg aaaactagat acatggtaag catgaaaaga aaatcaattt
     8821 gagcagatgc agtataatac atagcaattt aaaagctctc agaacgcttc tgagagcttt
     8881 taatatattc tttgataatt tgtctttctt aaatccatac gtcctaaaac aagctgtttt
     8941 tgcttaatat gatcatctga gagacatcaa atcaactttt ctctcaacaa atcattcaaa
     9001 gctttcgaca gattaatatt ctcttcagcc gctaaaacat ttaaatagtg ttccacagtc
     9061 actgattttc gatcgtaatt ttgcttgttt cgagctttag caactttcgt attagctttt
     9121 acgacaaata aattaacatt ctcagatcgc ttgattcttg catttaactc ttgaacaggc
     9181 gtaggcgatg gaatttcttt ttcttgcaac tctaaaagca ccaagtatcc ttccaatact
     9241 tcttgcgcca tctcaaaagc atcttctaaa gtttcaccat gcgtatataa gtctggaaaa
     9301 tctgggaatt taactgcata ttcatttcct ttccattcga atactgcaca aaaaaagtat
     9361 ttcatcgcat tgcacctctc taattaaaaa ataatttttg ctttttagtt tcaaaaaaag
     9421 aatcgcctac caaaaaaatt gataggcgat cacaagctaa ttaacctcta aaggagatta
     9481 ataccttgct cccaaggcac tacatatctt agcatacgtc tgaagcatct tcaaacgagt
     9541 aagatatatt ttgcattaac ctaatttttt ttctctataa ggtttttcat caaactcact
     9601 agaaatatta aactggacag caaaattgta atttctgcct tttctaactt tagcaaaatc
     9661 aatcttcagc attgattttt cattaatttc ctcaactgat tcccttaaaa cacgtctatc
     9721 aaaattcgat attgttttgt actcattttc tgttttcgta atctcacgaa ttttaactat
     9781 ttcaatcagg ggattcttat atatttcagc tttctcaaaa tctcctttat ttttggcgat
     9841 aagatatttt ttataataca tcgccaaata tttgtataac acaatgctat attttttttg
     9901 aaagcttgct aaatcttgaa aaacaaaata tggatatttc tttttgattg aaagaattag
     9961 gggaattact tcttgcgaga aattgaaagt tataatttgt tcttctaagt cccaatgaat
    10021 ttgatcaata gcagatactg tagcataagt cttatattct ttatctaagt tcgatatttc
    10081 ataaactgct tgtctctgaa gggtgttaac tatatttttt agttctgttt caagattttg
    10141 atcaacatcc atgtatttga ttattgcttt aacaggtaaa actatttccc ctgttttctt
    10201 taatttgtta ggagagatat aactgaacag caattgataa agttcatacg actttaaatc
    10261 cattttcatg tcaattgttg ctaagaactc ctcgttgtac tttgtcagtt catctttttt
    10321 caaaagcttt tccttccttc acgcaagttt ttcagttgtt aatcataaag gttcacataa
    10381 ggtaaataac tcgtaattga tttttcgaga tatttaacag tattttttat tgtgtaatcc
    10441 tccttatgag tagcaacgta agatagatga ttgacaactg attcaaatcc tctatctttg
    10501 gataaacgat cataaagcgg ataaacttta ttctgaagcc gactcataaa tttcagatcg
    10561 cataagtcgg cagaactgat caaaaagttt ttgattagta atttagtgta tttgctattc
    10621 atagctttca agtacgattg attcattttc tctgttttat cttctgcacc ttttaaataa
    10681 gcagggtctc tttgttcttg cttataaggt accggtactg acatggtacg tgtaagcaag
    10741 aactttatat ctgtataagt atgacccttc ttgataagct catagtcaat tttaaaatca
    10801 gtgtatttat tcagttgggc aatcggttct ttaattacac gtctttcaaa gtctcgaaaa
    10861 gctttatatt ttttctcaat actgaatttc tttcgtaact ctgcaattga aaaaacgggt
    10921 ttttttaatc tatcaagtga ttttgtactc catgttaagt tatctctatt tgcttcatac
    10981 agattaaaat ttttagtgaa taattgaaaa aaactgatcg catatttgct tgtcaagtta
    11041 actacttgat caaatgtata ttgtgtaaaa tgattttcta agttcgttaa caaaggcata
    11101 attgaactac taaatttaat agatactgca tcagaaaaat cattgaattc agtaatttct
    11161 agcggtgaga tgactttgta ttcagtctta ccgttaggtt tagttctttg aattttaatg
    11221 acagattgct gatgtaattt ttcaataact tgttttaatc ttgaatgttt tgaagaagat
    11281 gaactagaag acatcaacgc aaataaagcc tttttagata ctttgacggt attattaaaa
    11341 ggcggatttt taggatcaag atgtcctata gcgtaaagat aaattttttg tggcatagca
    11401 tccatttcaa caatgctatc cacaagttga ttcgataaag taacagttaa atcaaacatt
    11461 tttagattgc tttcggtagg ttcaaatttc gggtttacat tcttttctgt tattttcata
    11521 cgattgcctc ttctttctaa gcttggtacg agcttaaaaa cttggaactg gtacgattcc
    11581 attgaataag tatagacctg ttttcgatac gatacaataa gaaatgtatc gtaaatttat
    11641 aacataactc aatctcagta aaacgcattt tatttttatt aatcaaattt cattgataag
    11701 acagcagaaa tcgctatttt agaaccttta caaaataaaa atccttttca aaaaaaatac
    11761 gtaattataa aaattcacaa aattaaaaaa caaatatttt tttagttttt agaaataaat
    11821 tcacatactt tgtctttttt tgtatatatt cggaatacca catcgctttt tgtccacatt
    11881 cacatcgctt tttgtccatg ttcgcatcgc tttatgtcca ccctaaaatc tatttaagat
    11941 tgatataaag cctttttgga gattgctaaa gttttaaaga ttaaaatata tattataaat
    12001 ataaataaaa ggggaagctg aagcttcctc cttttccttt ttttcaatta ttcaaaatta
    12061 cacaagcaaa ataaactgct agatagtgtt agttttggtt ttaaggtttt taatttttga
    12121 gattttgaaa taagagagga ggaatcctcc tttattaaat atctgttaat atatctgtta
    12181 aatatctgtt aatatatctg ttaggggggt cttaacccct tgggagagta agtattgaag
    12241 gggtatgaag gtaacacttt tggacagtaa gggtaacaaa agtggacagt aagggtaaca
    12301 tttttggaca gtaaaggtaa caaaagtgga catttgaggt atcaaatcag gataagacgt
    12361 taccttcggt tttcttgatt tgaaaatcga aaaagtagaa aactagatta gtaacaaatc
    12421 tttgttgact gttaccttta gttgtgattt aatttaggta acaagcagaa aaaaagcgtt
    12481 tcctttaatt tagtaccaaa ttaacatgat taaagggatg caacaagaag attggagagt
    12541 aatcaatgtc tcaagaagtt accgaaaacg ttattgataa tgatgtagat ttagtatcag
    12601 atcaaacaaa tgcaattgtg cgttataaca atggtttgaa cacaacagct ttgcgaggat
    12661 ttaaacctgt cgaaatggat gtgttttggg ctattttatc aaaaatgaaa cgtcaaggaa
    12721 cagcagaagt aacttttgat ttcaacgtct ttcgttattt ggtcaaatac gacagaagag
    12781 aaaaggacag tttttattct gtgttaaaag ctttgagtga caaactaggt actttaacct
    12841 accgctttga agattcagat gtctatgaac agctgtggct ttttcaaaaa ttccgaatag
    12901 ataaacagca agaaacagtt acaatcaagg caaatgagga gtttgaattt atcttaaatt
    12961 caattggcaa aaactttact cgttttgaac ttgataactt tacaaaacta agaagtagct
    13021 acacaaaaga gctgtatcgt catctcatgc aatatagaaa taaagagtgg cgaaatggct
    13081 attgggtagt caaagttgaa gattttagaa gagcgttaag tattccttca agttacagaa
    13141 tgacaaatat tgacaaaaga atattagagc aggcgaaaga agaattttta gctcctgatg
    13201 aaaatggtat acaggtattt gaatcttttg attatgaaaa aatctatgcg aagaaaggaa
    13261 gaaaagttga tcggctcaga ttcactttta gtgaaccaga atcaaacttg ccatccattt
    13321 caatgcataa ttggttagaa gaagactgaa aagaagcagg ttgtaatttt aatacaacct
    13381 gcttcttttg aaaaaagtat gggtaacact tttggacaat aaatgcaaga agtttttttg
    13441 tgaccaaact ttatattaga ttcctgtgat tttgtgtgtt agaaaagccc tgatagaaaa
    13501 gcaaattcaa acacttttta atatgtatgg gtaacacttt tggacatgtc aataataaat
    13561 atattttctt tcgtcttttg cctaactaaa ggtaacactt ttggacagta tagttttgta
    13621 aactaattag atcgggttta atgagatata tcttagcatt tatgtaagaa tgaaggtaac
    13681 attaatggac atgtcaactg tttgaacaaa aaaatataga atattttgat ctgatttttc
    13741 atttttccaa acgttaaaaa atagttagtt attttattaa tagatagtca tccttaatgt
    13801 ttgaaatagc agaaaataag atattgtttg ataaatgttg ttgttataag gaaaacttga
    13861 atttgttaat tgatttattt atgttattat gatactataa taacataaat gtgttacgcc
    13921 cgaacctcat tttacaaaaa tgaaggggtg agacaatgga tgctattaat tggcatgaat
    13981 ttgttaacta tatggagaaa ctactagatg atctgaaaag ttttagcatt gctgatgccg
    14041 taaaacatgg gttgccagtt agtacaacga tgaaaatgcg agggttgaag gttccggatg
    14101 aaattatcaa gctgaggact aaaacaatca ttgaaactgc aaaaattgtt gacagtatga
    14161 accttgattt gaaaggcaaa aaaacaactt atgagaaaga ctttgtacgt tttttaaaaa
    14221 atgttatcgg aaaatatcta gggaaaaagt cttttactga attgagagac gaagagattg
    14281 taccaactcc aatggttagc aaaattcgca aaaacaaagc cgagggaaat tactatcttg
    14341 gcacaatagc aaaaatcgtt gagtatttgg aaaataaaga gcaaaagaaa ggaacgaaga
    14401 aaggaagata gttcgtgtga taaatatgaa tagggaagaa tgtaccgaaa tgataaaaaa
    14461 acccgaacct agaattaccg aaaaactgac tgattgcttg ctcttttacg aaagggggca
    14521 ggcaaactaa tggaaaaaga tgtcaattca gcatccacta aagatcaata caatatcatt
    14581 ggaggattac ttggtgataa ggataagaga acacgtcatg gattacgcat tatgcttgcg
    14641 acatttctag gggtgtttct tttattaaca ggcttgggta gctacattta tcaattagga
    14701 aactataatt attatcttgt caacaataca acaccaatcg gctcatcatt gacgtttgct
    14761 cgatcaggag cagagatcac tatctcagat gtttggacag ataaaactag agatgtaacg
    14821 gtggtcaaac ttggttactc aggaaacgca agaaaattgc tatctacaca aggtaaaaat
    14881 tataacttgt atatggcaac aaaagaaaaa gatcgtccta agaagcttga aatgagttat
    14941 gggatgttaa gtaccgatgg tgatgctttc ttattcctaa aaggtaaatt agatgaaaga
    15001 gcttatcaag tttttattgc taaccaagta tcactaattt caggtgatta tacagctgat
    15061 gatgatacca aagtcaatgt gaatgaatca gattcagtca ccaaggcact ttctcagtat
    15121 tctatgaatg atgttgataa tgaaggagta atttcttttg ggaaaaaagc aaaaccagtt
    15181 aatactcctg ataatatcaa tttcagaata aatccatact cagatacaac taacgtatat
    15241 caaggaagct tccttaccaa agatggcgat attgattact caaaagttgt tgctgtgaca
    15301 agtaagaata atgttgtaaa taagctagaa gaccagttaa atgcttcaaa tactaacatc
    15361 aagaaaatcg aagccacaaa agctgagtat cttgaacgtt tacaacaaaa tcctgacgat
    15421 aaagattcgc aagaaagtat tcgtcaacta gacagtcgaa tggagaccga aattaaaaca
    15481 atcaatgata cacaaaaatt gattgatcag tacaaaaatg ctgactttaa caaagattct
    15541 tttggagata tgaagacaac atttaagtac ctaaccctga ctgactagaa acaaattgtt
    15601 cttattttag aggaagtgaa cacaaacaga tggaaaatag taccgagttc tttaaaaata
    15661 ttcaaaccga attcaatcat gctctcacac taatgaaacc accgaaacat aagttagtgg
    15721 ggcgtgatga cgaactccat gagttgtcac tgatcttaaa tagaccggaa acaccacaag
    15781 ctatcttgtt aggtgaagca ggaacaggta aaacagcact ggcaagtgaa tgggctaaaa
    15841 gacagcgaca agaggggaag aaagtacaag tatataaatt atcaatcgga caattgcgag
    15901 ttggaggaga tgacgtcctc caaacacgtt tggaaactat aattccaaga ttgaaggaat
    15961 acgaacgaat tttaaaatcc gaggacaaag ataatgaatt tgtcctcttt atagatgaat
    16021 ttcataaagt tgtcagttca tttgggagcg gtaataaaat tgggggagat tcgctgaagg
    16081 aacctttagc aaatgaatca cttcagttga taaccgctac aactagaaaa gaatatgaaa
    16141 actatatcaa acgagatgat gctttaggta gacgactaca accgttagaa ctcaacgaat
    16201 taaaaagtga tgtcgttgtc gatatactaa gacaatggtt gaatacatca tctgtcaaag
    16261 atgatcctga agcttccttg aaattatcca aaaagattcc tgatgaattg cttgaagaaa
    16321 tcgtacaagc aaacaagctt tatcgtgagg atttatccga accggctaaa tcaattgccg
    16381 taatggaaga tttgattgcg aaggatcgtg tagatggcaa tgcgtataat cgagaaagcc
    16441 tgttacaaat atttggaaaa tataatattc aactagattc tgattttgat atagcaaaat
    16501 tggaagagga gctaaagtcc attaaagggc aaccagtagc ggtatatacg gtcacgatgt
    16561 tgtttaaacg aatgaaactt ggatttgatc gttttaaagg gcgaaagcaa ccaaaagcaa
    16621 cggctttgtt tacaggatca acaggggtcg gtaaaactgc gatggctaaa gctgtagcaa
    16681 gagggctata cggtgatgaa aataaagtcc ttttagtaaa tatgccagac tataaaacta
    16741 aagatgatgc tatgcggttt agaaagctta tcggaacaca tgtccggcat gatccatcag
    16801 caatcattgt ttgtgatgaa tatgaaaaag caggcgtaga aacacttgat gcctttttat
    16861 ctattctaga tgaaggaaga atcagtttta ctgaaagagg atgggatgat cgagaagtta
    16921 atgataccgt ctcactttca aatgcgattt tatttgcgac aacgaacgca ggggctgaga
    16981 tgtacgattc gatgaaccga tacagctcac tatctcgtgt tgctaatcag gaaaattcaa
    17041 atgagtacag cactgagctt aaaagagagt atcgaaaggt agcattagag attaaacaag
    17101 ctctaattgg tgaaggtcta aaacccgaag tcatcaaccg atttggacgg attgttccat
    17161 tttgggcttt atcggaacaa actcttttgg aaattgcaca caatgaaata ttgaaaagtg
    17221 ctgaattggt aagaaaacac ttaaacgtag acttacgcct tccaccacag aaaaaacatt
    17281 tctatggtca agaattttat gcagatgatc tctccatgca cttagttttt gatagagctt
    17341 cacaagaaga ttctaaatca gggggtgcaa gaaatatctt gcatctggtc gaatcagaat
    17401 ttgaagaagc aattactgaa gcaataatta cctatcctaa cgccaaaata tttgacgtta
    17461 aattgagcaa agcaggaggg tttgaaggga aaggcgatac ggatgcagaa attatcgcta
    17521 aaccttccat cgctgaaagt aaagaaaaca agtttgtgtt ttcggttgga ggggtgtagc
    17581 tatgaaattt aaaaagtctt tacttcaaaa tcgtaaaatt aagcgatggg ttgtaagagg
    17641 tttagttatc gtgggggcac ttgcagttac tttaggcgct ccatttggaa tgaatgatcc
    17701 aacgtctgat gatcaaggaa ataaacaaga aacagttgca aatacgaatg tggcttatgc
    17761 tgaaagtaaa ccggcagaaa aaggggtatg gacactatat gctcgtatcc tgatgggaga
    17821 taaagaggtc aatgatagtg ccactaaaac tggtcaaatg aatttacttg gtcaaggagg
    17881 tattagtgct gactttcctt attctgatat ttccaaacaa ggtaatgcaa ttgccggtgg
    17941 taacgcaggt acgcaattag catcaacctt agcgacttat aattactatg ggtatatcga
    18001 aacagtttca ggaaatgcga ttgcaagtca ggcatcctct tttattggtg gtatcggtag
    18061 aacgcttggc agttttatcg gatcaattag cttgatactt tctaatattg ctgaatccat
    18121 caatgaactc cttggaaaat ttttcatgag tatgaatata ttttcactta tgggttgggg
    18181 taaaggtgga tcggatgttt ctaatccggt agctaaggcg ttaactagct tacttaataa
    18241 tttgggatta aacaatcaat tatttaaagc gatagcaagc ttatctatgc tcttttttac
    18301 agcaatacta ggctatcaag gaatgaaact gttgtcgaaa gagggtgtta actcaaaggg
    18361 attatcacca gtacttgaaa aatggattgt tcgtattttt gtattgtttg tttttctacc
    18421 agttgcagga tcgttattgg gagtttatat caatggtatt cagtcagcaa aagaagatgg
    18481 aaaattacca acaaatgttg ttggtgatta catcattaat acaagacttt gggcttctac
    18541 tcaaaatctt gctccatcat ccgatactgg tgggaatgtg ccaaacggaa aaccaacatc
    18601 tgaaattaaa tttgttgaca ctgattatga accatctaaa aaatcagaaa tgatcagaat
    18661 gatcaatact agcagttacg ctagacaaaa taatgatcta gattccaatt cagtcggcat
    18721 ggcattgtta aaagaatggt caagtaatga tacgtttaat gtcaatactt atgccggtga
    18781 tgtgaatgta gctgaaaatg atgcttatat gggaagagcg cctgctgttt ggacaggcta
    18841 taatttaggg gatggaaaag aaggaaatgc aattaagaaa cctaccaatt taaatggtta
    18901 tatttggtca gctaatcaaa acgttacaga cgacaatcgc aagcctgatg gaaaaggaac
    18961 aacgaataac tttgatgcta ctcaaccttg gggtgttgct aacaatgcaa cgtttagcac
    19021 acagtcggta gcgttgatgc tccaatcagc actggataca tcaggggcta agttttatgc
    19081 gtataatatc gctccatcag gaattcaagg tagtgctaag aatatgtcaa cagtaaagac
    19141 agaatggcgt acagcttctt tagttggtca tggagctatg ggtaaattcg gctcatggtt
    19201 agcgatgaac gctgaatcaa tcgtattagc agtgctttat ctagcttgtg cttgggcgat
    19261 gtttaacgtt aatattgtaa aagccttcaa acgtgagttt gtgcgagttt gggaagtagt
    19321 cttaacaggg tctcttgccg caggagcagg ggttatggca atctcattag gagtcgtaac
    19381 cacatctctt atcggattac aattacctat ttttctgatc acctttgttc gtgcatttgg
    19441 agatacggct gtaggattca tccctaacac attacttggt gatggtgtag caaatattgt
    19501 acgatccgct atggtcttag gcgtaggata ccttctagtc tttaagaaag gtgaaaaggt
    19561 taataataat ttgattaata tgcttattgg attaccactt gaagtagcgt ttggttttga
    19621 cgaacgtgct aagttattat ttggcaatag aaatgatgct gttcgagttg tggatggcgt
    19681 ttctaaagca atcaaaaaaa gccaaggggc taaaaatcct tattatgatg gtggcagagg
    19741 atcagtaaaa gatatggtcg caggaggact tggagcagtc ggagcgcaag gaattgctga
    19801 tcgtttgaac aatatgggag acagcaatgc tgatactaag acaatcgctg ataaagttgt
    19861 tcctgaaacg cctgcttcag taccatttaa tggtggcgga gttggtggaa caatcaataa
    19921 tacctctttc aaagatttag ttcgtcaaga tcaattacgt aataactcgc aacaatatag
    19981 cgatcatcta ggacggatgg ttgatgaaaa aggaaacttt gtcgatgaaa atggtgaagt
    20041 attaaaagac agtaaatttg gtaaagatgc caaaggaaac tttattgatg acaaagggcg
    20101 tttggtcgat gaagatggaa atttgataga tacaaacggt aaaccattgg aagacaatga
    20161 agatggaaac atcggtatgg atagccaagg acgtttaatt gatgacaaag ggcgcttggt
    20221 cgatgacgaa ggaaaccttg ttgatgaaaa cggacgtaaa ttacttaacg gtgattttgg
    20281 gcaggatgta aatggtcgtc tagttgatga taaaggacga ttagttgatg aagacggaaa
    20341 tctggtcgat gagtttggta agaaacttgg aaaacacagt aacggttata acgtaaatgg
    20401 cgatacagtc gatgaagatg gaaacatcat tgattcaaga gatgctcttg tagcagatcg
    20461 aaataaagaa ctcgaacaac aacagcatac accaattgcg gttgcaatcc ctgatgatga
    20521 aagcgatgaa gttattttag atcaagcaca tcaagggtta agagaagaag caaaggataa
    20581 agttgtttca gatcaagcac aagaagagct aaaaaataaa tcgaatggtg aagatgtttc
    20641 aaaccaatat catcaaacgg ctggaaatgt acctgttgaa ggtcagtcta atgagcttca
    20701 caacaacttt gataatgttg ctgatcggaa tgatacagtc ttaaaagaaa aagaatcatt
    20761 aagacaagaa acagataaag accaatcaaa attgaagaaa acaactattc ctactcctat
    20821 tggttcaaca gttccgaagg tcaatcaagc taacgattct gaaacaaaca ctgggttagg
    20881 aatggcgaaa gacaatcaat ccattagaga acctaaaaag acgactgaaa gtagcaagtc
    20941 gaacaagtct aaatctactt caaataagac agggaaatct aaggaaccat ctaaagctaa
    21001 atcagcagta gaatatctat ctaaaactgg taagaacgtg tatacttcag cagataaagc
    21061 tttagcaaaa gcgccaaaag gaagcttagc aggaatgacc tatgaagcta agaacgaagc
    21121 tatcaaatta ggtaaaggaa ctgctaaagt tgccaaagta actgctaaag gagcaactaa
    21181 aactggtaaa gcagtgaaga aatctacgat gtcggtgaaa gattatattg caaaatatag
    21241 cgaacaaaaa ccaaccgata aaaagtctta agaagaataa aaagagggag cattaatatg
    21301 ctccttcttt tttgttcttc tcttcaaggg gaatgtcaag aaaaacttaa ttgtcttcaa
    21361 aaatttcttt tttatttggt agtaaatcta tgaaatattt gcttttttag ttatcggaga
    21421 ataagagggc ttatgctagt tagttaaaat caggtaacta gtcattaaat gctgataaat
    21481 aagcaatttt tacgaaaaaa aaaagaaaaa aatttgaggg aaaatacctt tcaatctaca
    21541 aaaatttatg ttattatgat aacataaaga tgtaaatatc ggagcatctt ttctgtattc
    21601 cgatcattga caagtgaaca gagaaaagtt tgcgatgata taattatctt gtgaaaaata
    21661 atcgaaagga aggatttata atatgtttga ttctattaag aatttggttt ccggaatgac
    21721 gccagaaatg aaaacgttgg ttcaagctat tctatattta atggctacat tttttgcatt
    21781 gaaaccagga ttcactgcta tgaaacattt cggtgatgca aaatggggag aaggcttaaa
    21841 atatgttgga gctgttgttg ctatttttat tatccctgta attacaactg ttatgttgtt
    21901 aacgatgggt actaaaacag gtaatgactt aaaccaaaaa gctaatatga tctcagctct
    21961 tattcctgtg gttggtattt atctagttac aaagtttcca aaagaagaac aattaggtta
    22021 aaaggtaaat tgagcaaatt tggatgatat gccgatttgt tcaatttact ttttgctaaa
    22081 aaactacact cgaagcgtag tttttgaagt gagggaagac aatagatgta agatggacag
    22141 agaaaagatg ttggttcacg caaacgaagt attgacgagc ggttttaaag gtaccgattt
    22201 ggcgaagata atgaatatga acgtaaacca gttctatgat tatcgaaacg gcaataaaaa
    22261 gattgaaaaa gccagacttg aaacgctcat caaatttgaa aaagcgtatc tatacgtgtt
    22321 agataaacag aaaagaacta ttgaccgaaa aaaaggagtt ttacaataat ggcaaaattc
    22381 aaagatatta ccgaatctat taattatgta gcagaaacag ttcccgtagt tgtactctct
    22441 ttgaagccac aattactcga agtgtaccaa aaagaagaaa aagaagcgaa ggcaaaaaaa
    22501 gctaaagcaa aggtatggaa agaattcgat gcgttgctga ccaaagattt accaaagaag
    22561 aattacgtgg tgattgagga cgtacaaaag ctcagcagtg aggaatttcc ggatgaggaa
    22621 gatgcgattc tagattccga aaagtacgtt gttttattaa aatcaagtct gaattatgtt
    22681 gttgatttac cggaaaaatt caaagaaaag ctattagctg gtgtccgctt tatggatagt
    22741 aatggtcaat tattagttat tcctgatgat cctaatatgt ttgaaagaga agttgaagaa
    22801 gctacagact tctacagtgc agtagatcaa ttagaggtag ctactaaagg cttagaaaga
    22861 attgctgaga ccttcaactc ttctgatgcg gttgaatttg aagctgtagc agaagccgaa
    22921 acagaagccg taccagatga agaagaagct ctttttgagg ataatgacga agcaactgaa
    22981 gcaggaaacc aagaagcaag catagaagaa acacaaacag aaacctacca acaaccaaaa
    23041 attgatgaaa aacccgaatc ccagagtgat aaagaaaatt atgtttatga agctgaaaaa
    23101 atcgaatctg aaagtgatgt accgaaaaat ttagatgctg atttgttaga aaaagatttt
    23161 gaacagatca tagccaataa atttcaacct gtagttttag atgaggttag tgatcctgtt
    23221 gatttgaacg aagagatgcg atccattgca gagttaaaac cattgctgtt tttagctaaa
    23281 gagagcatga acgctcgtat ctctattgct aataataaaa tcaacagtga attaaataaa
    23341 acgaaagagc aattgctact caaaggaaag caaatgttag cggatgagct tttgcggata
    23401 gaggaagcaa catcactaag tggtcaaaat cagtttacaa atacgctatc ggtcgctaaa
    23461 gaaagctacg aagcagaagc atctaaaatt gatgatttag tgatggaaaa atccagtatc
    23521 attcgtaaag attatgatat ggcgaaacag gcttatttgg aagcagaact aaagaaacta
    23581 gagcaacggt acgatgctga acatctgcca gaagttaaac aacaagaaga tgaatttaga
    23641 aaagcaactg aagaaagtct tgatcaacaa taccaagaag caattgaatc tattaaaaac
    23701 gatgctttga aacaaaatca agaagcaaaa gaagttgtgg tggaacggat catcaattca
    23761 ttgaaaccgg aaattgaaaa atctttaaca gaagtgcaag aaaaaacgtt agagattgtc
    23821 gaaaaaacaa aggcagaaaa tgaagctgag ttccacgata caaaagagaa aacaacagat
    23881 gtggttgtca aatattttgc gaacaaagaa gaagttgaaa agctagtagc taaagatacg
    23941 aatgagttag aatccaaact tgttgcacag caaaacatta ttgaagatta taaaaatcga
    24001 gtaaaagagt tcaaggattt agcagatggc tataaattaa atagccaaaa attgactgaa
    24061 caaagtgaag ctttacgaga agatttatct cgtgcaagaa acgacctatc acaaatggcg
    24121 gaaaaaacaa agtcactagt agttactaca gaaaatcaaa ctgagtctaa acgagatttt
    24181 acaagcaaaa agatggcgac tatcatcgct gtatcagcaa tggcttgtgg aacgattttg
    24241 ggctcatcct acttcattag tcataatcac caagaagccc aagctcaaac agaaacctca
    24301 atttcaagag tggaaaattc actaggagca aatacagctt ctacttcaac aacagacaac
    24361 aactatgaag caacaccaac gacagaattt aaagtcggag aagatgttgt catcagtatt
    24421 gatgggaaaa ctactaaggc tgaagtactt tccattgacg gtgataaggc agttgtgaaa
    24481 gctccagatg ggaatcaata caaaatacaa gctcacttaa ctgaaaagaa ttaaggaggg
    24541 ctaaaatgcg agaagaaaat attgtttatc cagtaattga aaatcaagca aatacaaaat
    24601 cttctcaact tcttcaagaa gtgcaagtcg tttatccggt taatgaacta ccacaaacga
    24661 atgaaacagt gagcaagtca atatcattaa ttggcttgct tattgttgca acagcgctaa
    24721 ctgttggtgt ttatcgtaag ctgaaagtga aaggaagtat ttctgatggc agataactca
    24781 aactatggat ttaaacataa tttgcatcaa aaaccttctg aaacaatagt cagagttgat
    24841 tggcttgaaa aacatgtcaa cagcacctat tctgttacag caaaaagact aattgtttca
    24901 gctattgttg ttgcagttgt tgttcttggt tcaatcgttt atttggtttc cgatacgatt
    24961 aaattgagat ttatttttat tccaacgcta ttgatgataa ttggttttct tatactgatt
    25021 agtcagtata tgtttattga tccatctagc ggatcactga tctcaggctc tattagactg
    25081 tttaaatcag tagttaacac agcaagagta acgatgggga aaaagaaacc atttcgagga
    25141 acggagattt tgaaagttag acaagatgga acgattatgt atcgagatgg tagtgtagca
    25201 ctaatgttta atttggatgg tgctacctcg ttaaccgcct tcccagaaga aatcatagcg
    25261 caagagggca aatcaattca ataccaaaat gcaagggatc gttccttggc tgaaatcaaa
    25321 attacttcat cacaacctca gaatacggaa cgtcaaatac ggaacatgaa agtacaaaaa
    25381 agaaatgcaa agagcaacaa agctgtgttt gacatagtag atcaacaaga gaggtttgta
    25441 tctgaatata ttgatggggt caaaactacg gtgatccaat atcttatttt gaagtcgcaa
    25501 tctcagatgg tattggatga aggcgtagaa cgtttagaaa attacacgag tagagggctt
    25561 tactatagcg ttactcagtt gtcagctaaa caaaccaaag aaatattatc agaaatttat
    25621 gcactgaaat aatagaggtg agaaatttaa tggaactaat gagtagatcg aagctactta
    25681 atttcagaaa aaagaaacac aaaaaagagc aaaatgctga tagttctaaa gcggtgaata
    25741 atacttatgt cagtgattta acatcattgg ttggttatca atttacacca tcttacatta
    25801 ggacaggtaa tcggtgggga acgattgtta aattagtgaa tacgtatgga atgaacagaa
    25861 attatgaatt tggatggatg gttaaagcta ttcctgaagt gaaggttgaa ggagtaaaag
    25921 cttacttaat cgaagcggat aagccagttt cgcaaaaaga tcagcaagaa atcatgcacg
    25981 aaaatgtcgg tggatcaacg aacagttatg aaaataactt gaacgataaa aattcagatg
    26041 cagaaatcaa taaaactgtt atgcgaatca ttgatttgaa tacagcatcc ctgcttgaca
    26101 gtacggcaaa caaaattatt gattggaagc tagagatcct tttagtttcc gattctcctg
    26161 ataaaatcag cgaacagttg attaaactgc aacgcctata tgatgaaaag atgtatggat
    26221 taaaactcat gtcttttggt ggcgatcaac gtgatctgtt tgatagactt ttaagcgaac
    26281 cggtgggaag tgtttacgaa tacacatcta tgagttctga ttttgcagga aatgatcatg
    26341 ctattcgtaa aggcttagat gatgcagatg gatgggcgat cggtacgatg tctacaagct
    26401 acgcttcagg tgtagcgttt atggcaatgg atcgttcttt taagggtaaa gtcttagtag
    26461 catcccacaa agatgcaaca atcaaaggct atagcgaaga aatctcagca tcatcacttt
    26521 gggggcaatt gattgctaat aacgcaatgg caaatcataa acggacgttt cacatcgtat
    26581 tgaataaatt tgactacttt ggtgatccga caggggaaac atttgttgct aagccatctt
    26641 taaatagtgt gatagagaag attaatctag ctgaagaagg acttaattct ttagagcttt
    26701 ttggtgatcg aaaggatatt ggagatttat atagacgtaa tcttgataaa ctaactcaga
    26761 ttttctatct gttagcggac agagaattaa atcctgcaac aagagtattg ctgaagaaag
    26821 agttggggaa cttttatatt agtcagaagc tttgggatat taactatgaa gagcggtcga
    26881 aactgttagt aaacagcagt gatacaatgc cggttcttgg ggatttcatc agctatttaa
    26941 caaatgctga gacaaaagcg aaaaaatccg gtacagaaaa agaaattgat cggattattg
    27001 tattaaaaga tacattgcga acagcattgg atagcttctc taagatgttc aatcagagaa
    27061 ctacattgaa agaaattggc aagagcgaca aattgcagat ttactacaat ttgagcgatt
    27121 taagaacaga aatcaatgtc atggaagccc aatttttaaa tacctttgac ttcatcacgt
    27181 cacaagctaa aaaaggcgat atcgttatga tccatgggtt tgatagatta tcagttgaaa
    27241 ccgttggatt aattaaaaag cgactaacaa tgcttgaaga agatggagtc cggcttgcgt
    27301 ttctctttga tactatcgga tcaggagaag ataaagacgg tatcgaaaca gctgatattt
    27361 tcaatactga tggaaaaatt tatcaaagtt ttgaaagtga ttttaactac accatcatcg
    27421 ggaaaatgac cagtgaagaa ttgatgaagt atgaaaagaa ggttgctcct gcaagaaaag
    27481 gagaagatga tcaaaaacta tcagtggatt tgagacagta tttgacatca ggtcgaagca
    27541 ataaatttca aatcagaaga ccagtcgatt tgacgaacaa ttttgttatt ggagagttta
    27601 ttatttaagt tctattcttg tttttgaata tttatggcta agaaataaca tgcttatata
    27661 ggactaagta aacagtttta gagaacgtga tacctatcat gaaacgagag ctatagtaat
    27721 ttcacaaagt gaaaacttac tgagagaaag aattatagtg gaggtaaaat aatgaaaggt
    27781 ggttgaagca tgaataattt accagaaaag aatcgtgtat ttagattgaa gggtgatctc
    27841 agccaattta aattagatga aaatggagaa ttttttgttt ggatgagaca tgaacattgt
    27901 tggtgtaatc cttttgagtt aagaaaagaa aacgaaatta gaaccaaaat tgaaaatgga
    27961 gagtgggatt acctcttcag gtggtcagac gaatctttta tagcaaacaa catggatcat
    28021 gcgtttgttt catttctgga taatacatgg ggtacttggt acgattgtta cctaaaaaaa
    28081 ttgggatttc aaaatattga tattgatgct attgagtgtc ttaaagaagt cgataataag
    28141 tatgaagaag aaccatatta tctcttacaa caaaaattag ccaatgattc agcctttcaa
    28201 gtagaagttt gggaaaacga agatggaacc tatgaaggtc tagcgcaaga aattccatta
    28261 tcagaaaaga agtgttcgat ttgaaaaaag tatatcaaca ttttttatgt attagttata
    28321 tcaccttatt tagctttatt tttctatttg gtgtatccgt tattgtcgga acaattttca
    28381 tttggcttac cgatgaagat atgtcattga agtggtttat gtgtctacta tcgtgcatta
    28441 tgttagttct tttatgttta gaagttcgtg aatttttgcc ttctcattgg caagaaaaaa
    28501 ggaaagagct tagagaagaa atcgaaataa aaacgagcga ctggatggat catacttggt
    28561 taccattgaa aaaaaatatt aagcaaaaag aattttgggt tctacaatta tgggcattag
    28621 gaaagttttg cagttatgtg gctctcctta ttttcgacct aatcttttac tctataggga
    28681 tatttggaac ggcttattac ttattaactg atcacaatag gcttgcagtg tcagacattt
    28741 tcacagtgtt aatgacattg atttttgtca ttcaattgtc tatttttagt caattactta
    28801 tccgatttat cagacaaaaa ataacaatag acaaactttt tagaagttta gtcgatttac
    28861 ttattataag tgtcattcta atgaccctaa cagaaaggat cgaacaatca ggaaaaacag
    28921 tagagggatt gaaggaattt ttgactatgt tcattcagtt tgtaaatggg catatgtatt
    28981 tatttgtgtc tggcattatt ctttcaatga tcggatatgt aactacgaaa cttattaaac
    29041 gctcaattga aaaagaaata gagattgacc tgattccgac actttctggc tatgactatt
    29101 atgtcaatta tgggaagttt gaggagttgc ttcatacgaa tattgttcgc acaagtattc
    29161 gagaatttac aaacaaggaa ataaaagatt ccattcatgg agagccaaca aggaagttca
    29221 ttgaccgtca aaagaaggtc tacaattttt atgaagtgga agcgatccta cgatatggta
    29281 tcggaaaatt gtggctagaa ggaagaaata tccgtcaaaa caaaccatac gttttttgga
    29341 aaagtggagt tttagaacaa tttcctagga gaaatagata aaataaaagg agcaaatggt
    29401 tatgaaacaa tatttaaata aatggaaagt gatagagggg tcattagtag cacaaagaat
    29461 aaagggatta cctgattgct tagagaagga tcatttgttt caaatacgag aaatgttaaa
    29521 aaaagaacag tttgatccag accaattttt agtagtcgag tatccaacta ttggggtgta
    29581 ctgttgtaac catatcaatg atggaaaata ttttattatt caggaatatg aaggacagtt
    29641 aactcctttc tatactacat gggaaatgag tgaggatggg atcaataatt ttccatgtga
    29701 aagcattgaa gagtcaatat ctcaaaccga atgttaattg attataacta taaaaaagga
    29761 ttgaacatta tgttcaattc ttttttcaat aaaatgtttt ccggcatttc attatttggt
    29821 gtaaattcga ttttcaacac agatggaaaa agctatcaaa gtttaatagt gattctaact
    29881 acttcattat cgggagaatg accagtgaag aattgatgaa gtatgaaaag agggttgctc
    29941 ctgcaagaaa agcagaagat gaacaaaagg tatcagtgga tttgaggcag aatttgacat
    30001 caggtcaaag caataaattt caagtcagaa gaccagttta tttgacgacc aattttgtga
    30061 ttgaccctca aacttttgga tgctaaaatg tgacatgaga tgatttgtgt gcattatggc
    30121 acaaaaacta cttcctgact gcgacgaaag gaagtagttt tttcatgacc tagaataaga
    30181 aaggcagtga atgaatggat acagctatat tgtatattaa tatacttcca tctatatcct
    30241 ttattattac tcttacattt ttcgtacgta aaaaaatttt ttttacagag aagaagcttg
    30301 tatgcttttt ggtgaaatgt ggagtagtat ttcttttttc ttggttattt ctgattttat
    30361 caactctaag ttttctaatt atattatttg cgactgtgtg tcttttagac tttggtgtaa
    30421 tagcagtagc agtaatgtgg ttttggttaa ctagaaaacc taaaaaaaag aaaaatggtt
    30481 tggagaataa acgattattc agattaatgg aaaatcattg aagacatagg catattggac
    30541 atattaagag aaaaaaacta acaatttctt gattttttga aaaagagtat atatatcaag
    30601 tcaaaaacat aatgatttga ttttgttata ttgaaataga tattaagaaa aagcattcct
    30661 tgttaaaacg caagggatgc tttttatctt cacttaatct gtttaatata atctaacaac
    30721 ttttcttcgg aattaatttt ttcacctagg tattgttcag acttagtaat aagattcaat
    30781 ttaagatcat ttaatatttc tggatacgta tcacttttcg attcagctaa ttcatacagt
    30841 ttttttagtt ccttatcttt taactgaaac ttctgatcca tagtgacagc atttggatat
    30901 agaagtccat tataatgtgt gcgtaaatcc atgtggaatt tttgtttata aaagtctagc
    30961 agattagaaa taaaagaaga aaaataaccg ttatggtaaa actgattatt tctatttaag
    31021 ttatacaaca atttattaaa tttatcctga tttaatttgt cgtaaatcca tggcaatact
    31081 tccatgtcct tatctgagaa tttaaatgca ggagaatcgg tcagatgctc aatcataaaa
    31141 tctaagaaca tctctttttc gccaaaatat agatattgat tatatatcgt ttctaaggta
    31201 gaagttgcta gagaagagac tctcatgttt tgctgaagta ttggatcaag tagtctattt
    31261 aagtgttctt gtaattcttt cttggtgttt cctagaactg caagtgaacg tagacggtag
    31321 taatcttcta tatgtgttgt tattggctgt tttggttcaa aatcctttat tgatattttt
    31381 aagttttgaa ttatttcttc tgaaaaatca cgctgtaatt ctggatgact agctataaag
    31441 gtaagtaaaa agaagccttt tgtataagtt atctgttgat tttctccaag tgatttatta
    31501 atttcctctc tcgattcagc tgtgaacctg tagtttgaga tttctttttt tagaattttt
    31561 aaataaaatt ctttgctatc aaaaatcagg gcatctagta gtctgaaagt actcactcta
    31621 tctctatcta cttcttcatt cccataaggt aatttaaaaa caaacttaaa ggctgtttta
    31681 caaaggtttt ctttggcttt ctcacttagt tttttcagat agctattttt tagtgcagag
    31741 tgaaattctt caggtgtaac agacatagaa tgtattcgac ttgcatattg tatgatttcg
    31801 ttcgttactt tttttcctaa tattggattt tttagaaata caatttcata ggatttccgt
    31861 atcacatcct gacaagtttc ctttgttatt tctcttaatt cccaagtgtc accattgtaa
    31921 gtaactactg gatgagctga ataatttctt tgatccttaa tatattttaa atcctcatat
    31981 tcgtagctat caattagctc agtcgaatta taagtttttt ctagtagctc catttcccat
    32041 cttggggagt ttggatcttt ttttcttaat ttagctattt cttctaaaat tttcttagaa
    32101 gtatcatcct cgtatatatt tgctaaatca gataatttat caattaagtc taaaacaagc
    32161 gcactgtaat tgacagctat cgctgaacga taatttccct gataaaaact acttctgact
    32221 tcttctaata aaggaataat tctaggattt ttaactttgt ttatttcatc atctaatata
    32281 aacatataac tctccttttt ttgttgatat gacaagttta ccacaaaaaa taaattaaaa
    32341 aacgagacat atttatgtta ttatggtatc ataataacat aaatatatgg aatgagatga
    32401 tgaaaaatgg gcttgaaaca atcagtttac atctggttat atcctgataa tcccatgtca
    32461 ataatggctg atatttttgt aggtagcatt ttattgtgtt tactgtatct aattttcttc
    32521 cacaatttaa tcgggtacat gatcctcgat tggaaaggga tgttcgatat agaaagaaaa
    32581 ccatttaata ataagcaaac ttataccacc tttgcaggtg gagatttgga agaaaaagaa
    32641 gttgatgtga cacatgaaat tggattagct ttatcaggaa ttcaagatgc tgttgcatta
    32701 ggatcaatga taacggatac cggatataac gcctatgaag tattttatct catgtatttt
    32761 atatggcgag atagtcaaga tattatcaac ggtcgtgctg aaaaaacaat catttcaaat
    32821 actgaaattt ctttctgtat gggtgaaggg gaaatgattg tatataaaga tggatcagca
    32881 aacattaaaa ccgcctttgg tgatattgaa acaagtatta aagagcttag ggagtatgta
    32941 cagaatccct ataattttca atacttagca ggaagaataa atttagttta ggagtaagaa
    33001 tatgaaaaaa attattgatc agccaacaaa tcgcttaata gtcgaaggcg ttattttcgt
    33061 aacaatgtat ttcactgtgg gaaatggtat ttcaaataca atcattcaaa aagacccaca
    33121 atacttgtta tttagtcttt atgcttggat tgtgcagatg ctttgggtgt ctttattaat
    33181 gctgttgttt tattggatag tacgggttat tcttttagtg ttaaacaaag caaaaaagaa
    33241 aaaggatagg tgagtaaaaa tgaaagcaac aaaattaaaa cttgtactat tttcaacagc
    33301 agtattacta actttatcag catgtggtac atcaaagagc gctgataata ccaaagacac
    33361 aaaatctagc agtacagtgg tgactgaaag cagttcagcg cagacatcta caactatgtc
    33421 ttcaatgact gagaacaatg ctgaaaaatt tcaaaagtat aaggacgtaa atcaaggtaa
    33481 aaagccggaa gaagtttata acgagtttat ctcttcagta aaaaatgatg aaagttatgg
    33541 gaatgatcca ttacaaaata aaaatctgac tattgttctt tacaaacctg attgtcctga
    33601 ttgccaaaaa gcagaatcca aaattatgca agaaatacaa gaaggattta gttatgctcc
    33661 aacattaatg cacgatatta ccatcggcaa acctgcttac gaatatcatg cagttgcggt
    33721 agatataacg gatggcgtac ctgaatgggt gcaaaatatc cctggtctgg ctgatgagaa
    33781 caaccaatat cacacgccaa cactagcagt agtaacacca aagaaaattg atggaacact
    33841 atactgggat ttaatgggaa tgacaacaaa tacatccgat gaaatgattg aaagcaccag
    33901 taactactat atggatttgg gagcctataa atcataccaa cagtctgaac ctgttatgga
    33961 tgatcaatct actattaagt agtttttagg gcgtaagccc tattttttta aaattatgcg
    34021 ataacaaatt aatgatttct gaaacaaacg ttcttactca actactcgaa acacacaagc
    34081 taattaacta gattataaag gagaatgcgt aatgaaagaa atcagtgcaa gtgaagccca
    34141 tatttctcaa aagaatcagg ttggtatata taaggtgctt catggtgaag ggatttataa
    34201 aatacagtta cttactggtt acaataacgg tgcaaatggg aagtggttaa aggcaacaag
    34261 tattcgtgaa cttagaagtg aagtagaaaa aattaggctt ggtctcacaa gtgaagaaat
    34321 agctaaactg aagggaaata attcttttgt aattgaaaaa gaaaaggaaa aacatctact
    34381 ttatcgtgag ctgaaaattg aagaatacta tgaaattgat ttacctgaaa aattaagaat
    34441 tattgatcaa cgttcagaag aaaaatggaa aactgaaata tatccaagct taaatgacta
    34501 caagacatca ggaaagccca tgtataacta cacaggcttt ttaacctaca cacaatacaa
    34561 atatgccttt cgcaaattaa aaggcattaa acaagctgaa attgccctgt atgagatgaa
    34621 aaaagtggat agattgaaat tgattcaaga gttagtgcaa aatacatgga atcaagcgat
    34681 tgtcgagcta gaaaaagaaa atgggaaaat tttaactaat aaatatctga tcgtaaatga
    34741 aattgaagaa ttaattggtt tattttcatg tgaaaaaggt gaagtcttct tgaagtcatc
    34801 accaacctat tcaattttgg acagtccgta tcagtttata caggcaagga gatgagttag
    34861 atgtctagct tgaaagatga ctatatccaa gcaaaacaag agtatgaaaa aatacagatg
    34921 ttgaaatctg agaaagtcga attattttta acacaaaaat tagcagagga tcatattgct
    34981 aacttagatg ctaaaacttt agaggaatat gatcggcagt ttaatcagat acatcacaat
    35041 atcattttgc ttgttgagca ggcaaagcga aggtttgaaa aagcggaata tgatctatta
    35101 gatcaaggaa ttgtatgctt ggataaggga ttgcgaagat attatggtgc agagttaaaa
    35161 aagcaaaatg tagatagaaa tgcactaaaa catgaaatct tagaattgat aatgcaacga
    35221 atttgaaggg atagaaagca aatgtcacaa aacattagtg ctgtaaaaca attaatagaa
    35281 gaagatttag agaccacagt tcagagatta gcggaacgaa tccattttgc aaattatcaa
    35341 accatctcta actggtcaag caggaacaga aatccggaag aaattccaat gaaatttttt
    35401 tatcgaatcg gaaaagaata tggtgttgat ccgataaaaa ttttcgagaa gaccatgatt
    35461 tattactatc atggaacacc taacgaagtg ttagaacatc ttttggaaga ggcagaagaa
    35521 aaatcatcaa tattagagct atcgcaaaat gacaaagtta ttcaagtgaa atgcgttgat
    35581 ccaacaggaa agattattgt cggttttgat attgaaagaa aatccaaaat agattttatt
    35641 actcaattaa aaaagataaa taatttagat attctaacgc ttagaccaga tcatgaacag
    35701 ttgtttatga gttttaaaaa acaattgaaa gaaatacaaa aaagatataa caaggtgttt
    35761 cctaatgcgt gaaaagctca aagaaatcag agttttttgg ataattaaca aagaatttat
    35821 catcttatat actcttggat ttctcagcat gatcttgttt tggtggttat atttaaaaaa
    35881 gtaattggag agggtcaaga ggatttttat caagataagg atgtggaaac tgcatgtgga
    35941 ataatagatt aaaaacagga ttgttattaa tagttatttc ctgcgctatg atgattggta
    36001 tgaggataca aagggaacaa tcctattttg aagtttctgc taacaacgta atagaaaaat
    36061 gctattatgg tcaacattat tggagtgagg aagtcagaga aaatatagat cgtgaatatg
    36121 ttcaaagaat tgtttgggat gcatattcta tcaaagatta tcctaaaagt ttaacgagta
    36181 gattatttta cagcgaaaaa gataatcaaa aactctcaga tttgatgatg aaaaaagtaa
    36241 ggaaacttgc acaaagctat ttggaagaaa aagcaggagc aataaaagat aaggaagaaa
    36301 tggagaaatt tgctattgaa tgaaaaaacg gaattatttt taaaaaaagt gaactttctt
    36361 gtagaaaaat attttttaac aaagcacaaa attatgtatc taataaaagg attgatcggt
    36421 ggaattattt tatggatact tactttcttt tctttatttg gtggatttac taattataaa
    36481 aagttagtag acgaaaatgt gaagcaaatt agtgcatctg aatcattaag acatcaagct
    36541 gaagatttag taagtaatcc attatttata ggaggtatgg tagaagtgat aataattagc
    36601 ttcgttattg gttatttagg tatgtgtatc ttccagataa tctttgatct aataagagga
    36661 aaagtctttc aaggatttaa atttgaaaaa ataaatttac gttagagata ataggctatg
    36721 agaggagaag aatagtggaa caaactaatc atattactga agaaactaga aaattcattt
    36781 gtttagaatc gttttattcg gaaggtaggt attgtaacaa aggtgaaact tatacggcat
    36841 atcctattga agggggattt aaattagttt ttgaaaacgg agacatgaat tttaccactg
    36901 agttgtttga gtgtgttgtt gaaacgtggt cagatgtgtt gcttgaagtc actaaataat
    36961 caatgttatc tagtagaatt aagcaactaa aaaagaaaga aggaagaaaa tatggcgtat
    37021 gaaaatttac aattagtagt agcaggagtt agtggtgata tatatcttgc aaaagcaaaa
    37081 gatggaatta tggacacgaa ctatagaaga gttgcgacag atgatgtgtt aagctcagcg
    37141 acagaatggt ttcgaactaa tgaagaatct gctgttcact ttaaaggaat tgatggaaac
    37201 attcatagtt tgttctatac aaaagacaaa gaaaaagcca aaaaaattct tgatattttg
    37261 aaggaaagtt agttaattaa ctaacgatat aagagaaggc gagaccaagt atttgcttgg
    37321 tctctatcat tttaaccact agtcaattaa ataaatatca aaaatgagta tggctaacca
    37381 cttgatcacg aacagcatcg ctacaaatac tgtatcagta tttgatagaa tacatattct
    37441 attttaaaaa aagaaaagaa atttattgat gatatacaca atagtgtgta taataaagag
    37501 gaagaagtga ttagaacttt gagcaactca atcattgctt tcgtaggaga ataatagaac
    37561 agttcctaag aagtccgttg ttcagtaaat ttttgagtaa aggagacgtt atgagcaaat
    37621 acgaattttt agacataaat gttggttctg cttttttgat taatcttgat atgtttgaag
    37681 cttgggcgca atcatcagga tataattggg catttagtga tgatatgatc gagtttttaa
    37741 gtcgaacgat gagttgggaa agttataagg tcaagcaatt tttagatgtc attgcaaaac
    37801 caatctacac atctgctgat gatcattggt caggcagaga gttggcaacc cttaaagaac
    37861 tagcagagta caacaaaaac tacaatattc cgtttcaagt ttcttacact acagatgatt
    37921 tttccgttat ttacgcatta ggtaaggaaa gtagatattt aatccctaat aggtacttag
    37981 agataaagat gaatagcgat tataccaact atacgctagg tgagttaaaa gctcttagac
    38041 agagcgacag cgaggaattt agcacgctca aaaatgaagt aagcacagag aattttcgca
    38101 tatcagatat tgaagaatta aaaaaacagc aaaaacagca actagaaaag caaaaaaaat
    38161 taattgaaga cattgcatat aaacgagtag atgagcttgc agtacaagaa gctaaaatag
    38221 aaaaaatgcg acaagaactc tatgaaaaac agcaatcaat gttagctgaa gcgaataaaa
    38281 tgcgctttga attggagaaa aaagtagaag agttaaatac taaaattttt tatttggaaa
    38341 ctgaaattga tgcgattgaa ggctattttg gtgaaagtac aacttttgtt cagttgcgtt
    38401 caggtcaaaa gagcttaccg gaagatccag tggtgatgtt tcaaaaagta cggtatctag
    38461 atgaagaatt aggaagatat ttcagtatgc gagaagttga aggtacagct gataccatgc
    38521 tgacttttga gcaggcgcta tcctcacgag atgatttgtt tgatttattc tcaccgaaca
    38581 aaaagtcaat ctcaatgata cgtatttcca aatcaggata tggctacact aacggtcaaa
    38641 tggaaagtac aaacatgcta gaaaagtata aaaaatatca tggagaaact ttagcaattt
    38701 tactgagaaa cggtgataac ttatggattt gttggttgga tgaaaataag atcagtttca
    38761 aagatgattt gttttaccaa aacaaagtaa cagagcaggc atctgaaacc aaaaatatgg
    38821 taaaggagat agattggaca catactcgta atttagaaga gcgattgaaa gatgctaaaa
    38881 agcagaagag ggctgttaga ggagagtttg ctagtcgtta tattcttttt tcaatcttgc
    38941 aaggtgttct agataatgga acgatgatga ccttaccaga aaaagtaaat gtgatccaag
    39001 caattaatca gaaaagccca tattttattg caagcgtagc ggatggatgg ttagaagaca
    39061 cacgcttgcc gtcatttagc gagttgattg ataaatacag tttctataaa gaaacaggtg
    39121 aagacagagt attagaagcg gaatatgagt tgaatcccga taaatttgaa aatatgagag
    39181 attatccaca caaacaaaat gatgacattt atgtgttata caggttacgt gggtggtcgc
    39241 ctacatcagc aaattatact tattacaaca gtgataaccg ttcaaatagt gagagtggac
    39301 gtgacttagc atattcgatt gacttgaaag acggcattta tcagattaat ttagtaaaac
    39361 agggaaaatt ccaaaaacaa atgttttccc gaacgtttgt tggagttgat tctactacag
    39421 gagaaaatat ctatgattac tattatgaaa gagaaacaga gtactatatt cgaggacaaa
    39481 aagcctacaa caaaagtgac tattggggtg acaaggcgaa gcgattaact gcaaatttta
    39541 gagtgtatga atccgaattt ataaacttaa cctttcttaa tagtgttttt attgattatg
    39601 tcattactac gaaacgtatt ggaagttatt caagtaccta cgcatcctta gttccaatgt
    39661 taaactcgat gagccgtttc ttaaaagaaa gagaagagaa agagctacag tatatcaaag
    39721 aaattatgcc agattttgaa atgcacgaat ggcaaattgc tttatcagat tggaaattga
    39781 cgaataaggt tcgtagaata acaccttttc aagccaagcg ctttttgaaa gctatgaaat
    39841 ttgttcaata gaaaaatcca ccagacaagc tgatggaaac taacccaatt actaacagtt
    39901 gtaattggta ttagcttaca agttaattat ggtgtttagt caatcccaaa ttgtgaaacg
    39961 gcaccaataa ttggaaagga aaaatgaaat gtctgaagat ttacgagagc tgaagttacg
    40021 aaagaagcag tatgatcagt atttttccgg tttgttgaat aaacatattg aagaaggact
    40081 tgatcatgat tttggaaaag atgaatattg tttgtattgc gagcgttaca atgaaaaaat
    40141 gtctgaatat ctacagctaa agaaaaagat acaaaaatta gagaaaaaag tacatgcttg
    40201 gaagtataaa gattccatgt ctggttatgg tgaatctcaa aataagccaa gaaaaaagaa
    40261 gcaagcgtat gttctaaaga tggtaaatgg ggaagagtta attgtattag cttacagtaa
    40321 agaagaggtt cgagaactcg ttgaaactcc tgtcaggaaa aagatagaaa caataaatga
    40381 gatagacgac agccaataca atagcatcta tattgaaaca agtcatggtg gatataagaa
    40441 attagcaact ttccttagaa gttctaaagt tgggataatt tctaaaaaag acataagtgc
    40501 tgttaccgat agtttttgta ctttatctgt tcctaaagca tatttacaat acaacagtaa
    40561 tagatttgaa cgcttagaga aattctgtag accatatact gtattgaaaa ctaaaaaatt
    40621 caataaaact aaaagagttt caatcaagtt accgactggt caaatctcgc taggcgaaaa
    40681 agattatctg ataaaagtag gaaaagagtt aataatcaaa gtgccggaac gcttatttaa
    40741 agaaaatttt gagatacgtg aaaccttggt atggaaagaa gaataatagt ttggaagaca
    40801 tagcagggta taaggaggaa ttaaaatggc agtaaataga aaattgcagg tattgattac
    40861 agacgacttt ggcggagcag agtggtttga tgtgcagtct atagattttg aagaagaagt
    40921 cgtagttata gaaaatgtat acggcgaaga atactcatat tcgcttgaag gagagaatgt
    40981 tgaggattta cggttgattg gtgagtagaa aatagcagaa atatcaaaat ggagatgaaa
    41041 tcagcgtgta tatgattaac aagaaaacag ggaaacgtat tttcagcagt aaagacgagg
    41101 taattcggga tttgagtgaa aactttgaac caattgaatt atttttccat cagttaaatc
    41161 tagaaattga aaaattacta aaggaaccta cagaaggacg aattaataaa gtattgaaat
    41221 taatagcatg taaagatagt catgcttaca tgctttatcc aaaatggtat gcagacaaaa
    41281 atatagctga aataaacgat tatctagcta gtataaagga acgcaatgca attaattaac
    41341 aatagacgaa aaatgaaatt aaaggagata ggaaaatggc agaagttaaa tttggatcaa
    41401 aagaatggtt tgaagagtta aactcaatga ttcagtttcc agaaaagttt actttttact
    41461 acaacaaaag agacaatatc attcaagtag ctttacttga aactaaggga agattagaaa
    41521 atactcgatg gagtatgata aaaccatcag cactcagatt tcatcctaaa gacttgacag
    41581 cggttgtagc ggtaaaagag gttcaagact cacaagaata ctatgaagta gtaaaaaatc
    41641 tgaaggatat ctggtgtaaa taattctaaa ttgaccagtt atttggtcaa aaatagtatg
    41701 tgattatttc atccaatatg gcatcataat tggtatgaaa ggggataaat tatttgaaag
    41761 aactagaagg tattaagaaa agaaatcagg aaagattgga actgttaaat gaaaatagaa
    41821 ataggatgtt tagttactat gaatccatga ggtgtttaaa taaatctaga agtgaaatta
    41881 tagaatcatc aaagtataaa caattgatgg aagagagagt ttctctcatt caagaaatag
    41941 aaacagagtc tatttttcca gatcattttc ctaaagtccg attttataat taaacattaa
    42001 caaatgaaac gatgctaaat aggcatcgtt ttttatctat ttgttaaatt tgtttattta
    42061 caaaatgtgt atcggtgtgt ataatatatt tgcagataag tcaagaaagg atgatctgaa
    42121 atgaaattag aaggtagctt acaaaacaga atgatggaag gcagaacaac aaataaggag
    42181 attgtagttg gtatgggagc aaccgagctt ctatataccg acagaaatcc ttatacagtg
    42241 attgaagtaa aaagcaaaaa cagaattttg gttcaagcgg atggggcaat caataaagct
    42301 acttttccag atcaagagtg ggaatacagc agaaatcctg aaggtcaaat attggaatta
    42361 attaagacaa aaaatggatg gaaagtgctt aaagaaaata ctcattttta tataggtgat
    42421 agagaaaaat actacgatcc tagtttttaa acaaactgaa gatgagcgag gttttatcct
    42481 cgctcagaaa ggagtatgaa ggatgttcag tcaagaagaa atcatcagca aatcgcaacc
    42541 ggataaaaat ggaactataa ccatgaagaa aaaaattcct tgctatcgtt gtagcggatc
    42601 aggtcagtac aagtttatgt ctatggggaa aattacctat ggcacatgct ttaagtgcga
    42661 tggtgacgga tttcttgttg taactgaaaa atcatttacg ccagaatata gagctaagct
    42721 aaatgcttta aataaaaaaa gggctgatcg aaaaataagg atcacaagag agaaagaatc
    42781 tcagaaaaat tttgatcgtt atggttttga aaagggttat atttacgtgg ttatagagga
    42841 aaatagctat gcgatcaaag atgaactgca tcatcaagga gctacttatg atcctttatt
    42901 aggttggttt ttcaaagaaa aacaagaaca atacaaaact caaaaagttt ttgtagaaga
    42961 attttccgta atcaatgatg aaggaattat tgaattgaag ctagaagaat acagcgaaag
    43021 acaacaggag attttaaggg cgaagcgtag agaagagggt gagtttattg gtacaatcgc
    43081 tcaacgattg gaattggatt tgaagcttac caataccgtc accatagata cacaatgggg
    43141 caatgtttat ctgcatttat ttgaagatca aaaccataat tcattgattt ggaagacaaa
    43201 taaatttctt tattatgatg aatgggatgt tggagaaata aggaaagtta aagcgacaat
    43261 aaaagcgcat gactactaca gagataccaa gcagacttgg gttaaaaatg ttaaaattca
    43321 aaaaaatgag tcagaaggga taaaagtatg aaaagagagg aatacaaaca aagattaaac
    43381 gaactgcttg aagaggacga aacattaact catggttcgc ctgatgaaat tctatacatg
    43441 attgataata tggtgatctt tggtggatat gaacttggta ataggtcagt tgatcataat
    43501 attttagaat ttgatgatgt atcatgggaa gaaattcttg attggggaat tttggcggta
    43561 cctgaaacta aaacctatat tagtgacact atggttcctt tttttgagga attggattat
    43621 aaaagacttc ccaaaaatga aaatcatata ctgggaggaa actaacaatg ttaagtgact
    43681 tatttatttt actgatctat ctaggtgtaa gtgaaattat tgcgtatttt ttgtttgtca
    43741 ttgctaatta tggtatttcc agtgcaatct ttcaaaggtt tagattacca gtaagtgaac
    43801 taaaaaaaat gatgagatta tcagcatatc ttttaatgtt tttgaatgtg gtcacgttaa
    43861 tctttgtagt aatcggtgtg actttcaatt taatggaata gagggaaatc tataatggaa
    43921 aaaagagaaa agaatgaaca tattttttga atcaatattg ttgatggttt ttgcgatcag
    43981 cgcttaccag aaatctaaaa aaagagtaat gattgtttaa tcatcactct ttttttgtag
    44041 ggaaattgaa atatgtggag cttgtattgg tttagttagg attgcttggc tataaggagc
    44101 tagttcttta tcaaaaccat caaagtaggt tttcagtagc gatttaactg atcgctcatc
    44161 ttgtttgatt tggataattt cagcatccaa atcggttgtg agatcaggag cttttgatgc
    44221 gattacctga ttttttttat cttccaactc tcttaggctg tttaacaagt tgtaaaaaga
    44281 atcattttga ttgttcattc caacgtgagt tgcaatttta acagaagttt cgtccgtatt
    44341 gtcatcaaaa gaactcattg gaatatcaag tagtaacgag gatgtttctt ttactgactt
    44401 tttctttaca gcattttttg tttttccatc ttttttgctc tttactcgag atgtttttgg
    44461 agctagttta ggtaaatcac ttgttttatc agtcatttat tagcttttcc tttctattaa
    44521 tttatgttat tatattatca taataacata aaaaaagtac actttttggg ggcgtagtta
    44581 ttgaaaaaat ctaagagaaa gattgccacg ttgcttatgt taatggtgca atttacaggg
    44641 attgcaatga gtgtaatcac aataaatccg caagtgatac atgctgatgg gatagctact
    44701 tatgatgcac aatttaactg tactactgaa ggtagtgggt caggaacaga tagcgatgga
    44761 aacgatactg gaagtggcgc tgtaacaggt gatgtagcca aaaatattga agatatttac
    44821 aaatacatga atggagaata tggcttatca ggtgctagta ttgcaggtat tttaggtaac
    44881 tggaaacaag aatcaggtat tgatcctcga gcagtagaag ggtattacgg agatggacag
    44941 agagaagttg acttcatcaa acaatttact gatggtctga tgggtattgg ctatggtcaa
    45001 tggacagctg ggcgacatac agcattagtt gattggtcta agaaaaataa tggcggttct
    45061 tggtggacta cagaagccca attggacttt atgttcaaag gtgatggggg attcgtagga
    45121 atattaaaag attatgcttt aaatgcttct gatgatgtcg tagaaaatgc agtaactttt
    45181 cataaaaact gggaaatttc agctgattcc gaatcagctg taatagctgt acgtggtgaa
    45241 aatgcaaaag aaatctggaa gtacatgaaa agcaagggta tggacggtgc aaaagatgaa
    45301 agtaagatca ataaaatatc cggaggttca agtaccggag atggatcgaa taattcatca
    45361 agttcagcat caggaacagt agaagatgcc tgtgcaacta cggatgacgc taattctggt
    45421 tctggatcaa ttgggaaaga aggaaaaatt aacggtaaaa aaggtcaagt aatagcgaat
    45481 atgacttatg aagaagcttt gaaaaaatat gggaaagcga tcacgctacc aaaattcaaa
    45541 gaaccagatt ggtcaacttc accttttaac ggaatatatc aaggtcagtg tacagagttg
    45601 acgtgggctt atatgagcca attatacgat gggaatcaac cgactactgg aaatggtggc
    45661 tatgtatggg aaagctacaa atcggcagga gcaaaaataa cgaacaaacc aatcgtaggt
    45721 tatgggttta gtgcttcgga tggatggtta tgggctgttg gagatgcagg tcatactggt
    45781 gtagttatag cggtgtttga agatggatca tttctatgtg ctaactttaa tgttccacca
    45841 acccaagctc caacaagggg agtttcggta acgttgattg atggagtgga cggatcagat
    45901 aatgtgcatt tctttagcgg tgtaggaaaa gctaaatttg aaatcgaaaa ataaattgga
    45961 atgaaggacg atatagatga atagtaaagt gaaagataca attatcattt ctttgctttc
    46021 tggtatgtta ttagcactgc ttgttggaat cctattcttc aataagcaga atcaggacat
    46081 agaagctgaa aaacacagtg tgcatattga agataagctg acattcttta aagaggttaa
    46141 accaaatgag gtggatggat tacggaaaga tttagaaaat gatattcaag aaatgttgaa
    46201 tggatcagtt caaaataagt ttgcagatca atcaggtgaa gaatttttga gaaaactatt
    46261 tattgtaaca ggtaaaccac caattactga taagagtagt gagaaagaaa aaatcgagta
    46321 ctataagcct tttactttta agctaacaaa ctttggaatt aagacatcta gtagtgggtt
    46381 gtatgaagca ttgaccgatt ttgaagttca atacaatgga aaaaaagtgt atgataccac
    46441 attaaccatc acactaaata atgataagaa agtgactggg ggtgcttttt atgcaaaatc
    46501 atgaaatcac tactgctaaa aaggcgatcg gaatactttc cataattgtg atcctttgtt
    46561 gtgtggtact atttcaaaag aaaaatgctt atgataataa tgtcaaagaa ttggaagacc
    46621 ttaaaagtaa acaaatcgaa gtagtcaaga ataaccagaa agtgaaagct atgcaggaag
    46681 ctcaatacaa agaattggga aatgctgaaa ttaaggaaca aagtaggaaa ttctttaatt
    46741 tattttacaa ctggaattca tgggaaaaat atacgaagaa tatgaaggaa atacaaaaag
    46801 aatttcctca gatagtaaag tctagtcctg ttgatatttc tggcacgatg gtaggcactg
    46861 gtaatagtcc aatatctagc tatactgctg attactatac aacaaatcaa aaaggtaaaa
    46921 tggttgaatt gattacacag acaagaaata ctccaacaac agcaacaaca acaatttggc
    46981 gtggagtaac aagcatagga gaaaatcaat tgttttattt ggaggattta gatagctatg
    47041 taagagcaga gtaaaataaa aaaagagcta acggtaccaa cgttagctct ttgtgctgac
    47101 gatgataaca aaacctaccg aaaaaggatt gtttcatcac aaacacaaac agaaaagaat
    47161 tgataaaaaa cccgaacctc agaattttta ttaatcaatt catttagatt ataataaatt
    47221 gagctgaaca ttacaatacg tatatgttgt aaaacgtaaa aaaagtcaaa agtaaatact
    47281 ttcttaaaaa gtcaaataaa actattaaag acaatatata tggtttattt gacataagaa
    47341 aatggtctag atgataattg taatatagaa ttgtagactt aaataaagca taaaatacct
    47401 tttatgataa ggtatagctc ttaattttaa gcagttttag atataagatt gaaagtaaag
    47461 tgggggagaa taataatggc agatcataaa ttagaaaagt tattagaaga aacgtggaat
    47521 ccaaaagaat ttagtgaatt ttttatggag aattttgaaa ctgacttagc agtgattgtt
    47581 aaagatgctc ttagagagca agggtatcct gaaactgcaa actatataaa cattaatttt
    47641 actttgtata ctgaaaataa aggaacttgg gatttttggg ctactttagc aaataaggag
    47701 ttaagtgata aatcagacac aggaattcgt aacttttttg aatcaaacag agatgattat
    47761 atgtatgcta acaatcaaaa taaattgaac tttagagtag agtttgatga aacacctgaa
    47821 gaatttatag aaagacaacc gcctaaagaa aatgtagcaa aggtattgga agatcgttgg
    47881 aattcagatg aaatagtttc aacaattagc gaactaggcg gacaatatga accgctagta
    47941 gaagcggtta gagaagagct aagactgaat aagtttcctg atgttcaaaa tattgatgtt
    48001 tctcaaattg aaataaacgt caagatcacg aataaacttg attatggtag ttgggcagat
    48061 atagcattag aaaaatacat ctactcaacg ttaaaagagt ttatcgaaaa tagaatggat
    48121 atcatgtatc tacagcatcc acagtattta aactttggtg ttgaaatagc tacacctctt
    48181 gaagaatgga aaatggagca agggcttgac tagcctttgc tccatttttt tattttacaa
    48241 atgaatacag ttcgtcgttt ttaccaagaa tgattgtttg cccaactttg agattgtcag
    48301 gtgatccttc agcggtttgt aaggagaacg ttccattgaa cttatcggat accgttatag
    48361 gtagctttaa ttctgatcct tcctcatctt tgagaattgc tgtttttcgt tcatcaaaat
    48421 aaacagtctt tattaaataa tttttcgtag tgatctcagc agatttgtct ttttttgtag
    48481 tagtaccagt ttctttagaa ttagaggtat tttctacgac tattccatct ttggcatttg
    48541 agcttgtgtt tatttgattg aatattttca gcgacaagat accgctaaga atagtcaata
    48601 tagaaataag tgtaattaat actgataatt tccttttttt gttcattttt tcttaacttc
    48661 cttttttgtt ttgattagtt agatattcct tatctaacaa gcatttttag gctattaaat
    48721 gaagatgaac cccaaaaaaa tattgcaatt atgacagtgc aatggtacta ttattataca
    48781 ataaattttg cgaaaggggt aggtctaaat cgcaaacaaa aagaaattga acctagacaa
    48841 atacaacttg tataccgcaa gggaagcaag tttgattttg ggtaaaggta gaaattatgt
    48901 tacaaacata attaactatt atccaaatcg tttgcctaaa ggttcgttca agaaagtggg
    48961 tgacaaaacc tatattataa cggaagaagg aatagaagca ttgcgatcac aaacagaaaa
    49021 taaaaacaaa aagaaggaat gaatatgaag aaaaattcgc ttggtatggt tttatttgca
    49081 tcagctgtac ttgcggggtt atcagtacca gtaacctcac atgctgatga atggactgca
    49141 aacacaccgg atcaaatcca aattacaaca ggacaatcaa gttatgcgtt caaaaaagga
    49201 gatactattt ggtcaactgt agagaagctt aaaagcttag gttacaagac tactgttgct
    49261 gagtttgccg aattgaacaa tgttgatttg gcaaaaggag aacaacggac tatcccaatt
    49321 ggacgaactt tcaacatcag taaagataca ttaacagtta aggaagcaga tggcgaagta
    49381 gttagtcaaa ctgttcaaaa ggataaacct gatcctgttg aaaatacgga gcaagagaac
    49441 aaaaaatccg agactacaaa agaatcggaa ccaacagcaa acacttctga taagacagaa
    49501 acaagtgctt caagtgttga tggagcaagt tcatctaata caaataattc tacaagtgag
    49561 aaagaagcat cacagggaac aagtgaaagt agcactacgg aagctgatac tggaacatca
    49621 acaaataact cacaatcaac atcaaatgat gcaaatgaat caaccagtac atcttctagt
    49681 gatcaatcac aaacaggttc gagtgatgaa actgacagtt ctacttcacc aagtaaaccc
    49741 gaacaaccaa ctaatgttgt gctaggcgtt ccatttatta gtcaaggtaa cacaatgctt
    49801 tgcgaaggaa catctttgct agaagcgtta cattataaag gtgttgcaac aaatcaagat
    49861 ttaatgactt ttgttaaatc aatgccacta tctcctgata ataatccaaa taatggtttt
    49921 tctggtgaat ggagacacaa tgtggatgga acttatcaag gtatgaatcc ggctcctgta
    49981 gtttcatggg gtgttgctaa tggcggaaat gttgtagata tttctggttc atcggtacaa
    50041 ggattaaaag atgagattgt aaatggaaat cctgtagtag catgggtaac ttatgccttt
    50101 gaacctgctc aacataaatc aatgttttgg ggcgatgcta tttggaatcg acatgtagta
    50161 gccgttgatg gatttaaaga cggtttttac catgttgtcg atcctgtgtt tggggcaagt
    50221 tggattaatg caggatcatt tgaaacagca tataacgtat ctcaattagc ggtttcggta
    50281 cgttaataaa agagatcgtt tatatactaa gtagttaagt atagtcttta tttgccctta
    50341 cttaactact tagaaaactt gttgctcatg gtgggtagaa ctaaaagggg tgatgctatg
    50401 aataatagag cgttgaaaca tcttgctttc aacgaaaaaa gtgattaata ggtgtggtga
    50461 ttttaactta ggaatgggcg cgaaggtcat taccacaccg acatatcgtg gctcattttc
    50521 ttcgcctaac tggtataaag aaggagtaac gagagatgaa gcatgaaaaa acgataattt
    50581 taaaatcaaa aataatttat tgttttgcga ctttagtaat ggtgttatct acgcttataa
    50641 tgcctttttc agcaataaga gctgaagcag atgtcacaaa accgacaagt gcaaaattta
    50701 cagaaactgg cgtaaaaaca gacggttata ttagggttaa tatgccgaat catccaaatg
    50761 aatggatgat ttcaagtcaa tttaaagatt ctcatggaaa tattggatat tgtatggatt
    50821 cagaattgcc atccccaaca ggaagtggag ccggaagctt gaagtacaag ggagctggca
    50881 gtgatgagtt ctatcgtatg tttaaaggtg gttttccatc aaaaacagca aaggaactgg
    50941 gtgcaggaaa tgatacagaa gcatggtatg caacacaatt agtttcatgg gttttagccg
    51001 gaaactttaa agtttcacag attgtatggt cacatccaaa ccataccgcc gcagaaacag
    51061 ctcgtgtaaa aaaagcattt gaaaaaattt atgactatgc taagaatgga aaagatacgc
    51121 ccaacactga gttttctatc acagcttcta agacagctga cgaaggaaaa tatcacactt
    51181 tcacatataa aacagcatca aataagactg gtaatgcgaa gctcactttt acatcagcaa
    51241 aacctgctgg tatgaagatt tatgatgcag atggtaaaga aattactaac aacactgtaa
    51301 aattaaattc aagttttact attaaagtac ctgtgactac accaagtggt actttgtcat
    51361 ttaaaggtac tgctaatgtt agcacgacca atccttttac ttttgatgga cgaggtgtgt
    51421 atcaagatgc tgtagttatg atcactactt cagaaacaaa ggactctaaa tctttgagtg
    51481 ctaaatggac aagagctgtt ggtcaggtaa cagttaagaa agttgatcaa gatggaaatg
    51541 ctttaaaagg agcggagttc actctaactg acaataacaa tgctaaatct gttaagacaa
    51601 ccggagatga tgggttagct acgtttgatt tgcaacaagg cttaacatac agcttagaag
    51661 aaacaaaagt tcctgaaggt tatcaaggtt catttaaaaa ggatggtatt actgttaata
    51721 ccgatggaca agaatttagt tacactgcta cgaacacaat taaaaaaggt aaaataaaag
    51781 ttaaaaaggt tgaccaagat ggaaaaccat tagctaatgc tgaatttacc ttgacggatg
    51841 attcatccca aacacaagca gaaaaaacag atgaaaacgg acttgcaagc ttcgatattg
    51901 tagcaaacaa gacctacagc cttgaagaga ccggagtacc aactggttat acaggcgcat
    51961 tcaagcaaga aggaat
//
LOCUS       DACEOE010000004        49927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEOE010000004 DACEOE010000000
VERSION     DACEOE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.004"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            52..1272
                     /locus_tag="IW774_000189"
     CDS             52..1272
                     /locus_tag="IW774_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1152570.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1269..3989
                     /locus_tag="IW774_000190"
     CDS             1269..3989
                     /locus_tag="IW774_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1152571.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3973..4917
                     /locus_tag="IW774_000191"
     CDS             3973..4917
                     /locus_tag="IW774_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1152572.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(4986..5996)
                     /locus_tag="IW774_000192"
     CDS             complement(4986..5996)
                     /locus_tag="IW774_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1152573.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6176..6520)
                     /locus_tag="IW774_000193"
     CDS             complement(6176..6520)
                     /locus_tag="IW774_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1152574.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6513..6947)
                     /locus_tag="IW774_000194"
     CDS             complement(6513..6947)
                     /locus_tag="IW774_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1152575.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7085..7822
                     /locus_tag="IW774_000195"
     CDS             7085..7822
                     /locus_tag="IW774_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1152576.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7815..9026
                     /locus_tag="IW774_000196"
     CDS             7815..9026
                     /locus_tag="IW774_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1152577.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9233..10012
                     /locus_tag="IW774_000197"
     CDS             9233..10012
                     /locus_tag="IW774_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1152578.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10082..10744
                     /gene="trmB"
                     /locus_tag="IW774_000198"
     CDS             10082..10744
                     /gene="trmB"
                     /locus_tag="IW774_000198"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1152579.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10842..12596)
                     /gene="ade"
                     /locus_tag="IW774_000199"
     CDS             complement(10842..12596)
                     /gene="ade"
                     /locus_tag="IW774_000199"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1152580.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12728..13657
                     /gene="cpaF"
                     /locus_tag="IW774_000200"
     CDS             12728..13657
                     /gene="cpaF"
                     /locus_tag="IW774_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1152581.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13614..14663
                     /locus_tag="IW774_000201"
     CDS             13614..14663
                     /locus_tag="IW774_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1152582.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14660..14938
                     /locus_tag="IW774_000202"
     CDS             14660..14938
                     /locus_tag="IW774_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1152583.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            14913..15383
                     /locus_tag="IW774_000203"
     CDS             14913..15383
                     /locus_tag="IW774_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1152584.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15400..15702
                     /locus_tag="IW774_000204"
     CDS             15400..15702
                     /locus_tag="IW774_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152585.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            15719..16090
                     /locus_tag="IW774_000205"
     CDS             15719..16090
                     /locus_tag="IW774_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1152586.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16062..16421
                     /locus_tag="IW774_000206"
     CDS             16062..16421
                     /locus_tag="IW774_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152587.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16593..17600
                     /locus_tag="IW774_000207"
     CDS             16593..17600
                     /locus_tag="IW774_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1152588.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17625..18809
                     /locus_tag="IW774_000208"
     CDS             17625..18809
                     /locus_tag="IW774_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1152589.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19246..20829)
                     /locus_tag="IW774_000209"
     CDS             complement(19246..20829)
                     /locus_tag="IW774_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1152590.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEVTVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21407..21550)
                     /locus_tag="IW774_000210"
     CDS             complement(21407..21550)
                     /locus_tag="IW774_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1152591.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21773..22252)
                     /locus_tag="IW774_000211"
     CDS             complement(21773..22252)
                     /locus_tag="IW774_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1152592.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22325..22609)
                     /locus_tag="IW774_000212"
     CDS             complement(22325..22609)
                     /locus_tag="IW774_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152593.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22687..22881)
                     /gene="ssrS"
                     /locus_tag="IW774_000213"
     ncRNA           complement(22687..22881)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW774_000213"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22953..24239)
                     /locus_tag="IW774_000214"
     CDS             complement(22953..24239)
                     /locus_tag="IW774_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1152594.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKSAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24377..25048
                     /locus_tag="IW774_000215"
     CDS             24377..25048
                     /locus_tag="IW774_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1152595.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25014..25502
                     /locus_tag="IW774_000216"
     CDS             25014..25502
                     /locus_tag="IW774_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1152596.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25515..26462
                     /gene="prmA"
                     /locus_tag="IW774_000217"
     CDS             25515..26462
                     /gene="prmA"
                     /locus_tag="IW774_000217"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1152597.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26463..27227
                     /locus_tag="IW774_000218"
     CDS             26463..27227
                     /locus_tag="IW774_000218"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1152598.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27460..29673
                     /locus_tag="IW774_000219"
     CDS             27460..29673
                     /locus_tag="IW774_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1152599.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29684..30130
                     /locus_tag="IW774_000220"
     CDS             29684..30130
                     /locus_tag="IW774_000220"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1152600.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30301..31560
                     /locus_tag="IW774_000221"
     CDS             30301..31560
                     /locus_tag="IW774_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1152601.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31649..32596)
                     /locus_tag="IW774_000222"
     CDS             complement(31649..32596)
                     /locus_tag="IW774_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1152602.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            32990..34282
                     /locus_tag="IW774_000223"
     CDS             32990..34282
                     /locus_tag="IW774_000223"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1152603.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34297..36066
                     /gene="aspS"
                     /locus_tag="IW774_000224"
     CDS             34297..36066
                     /gene="aspS"
                     /locus_tag="IW774_000224"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1152604.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36175..37050
                     /locus_tag="IW774_000225"
     CDS             36175..37050
                     /locus_tag="IW774_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1152605.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37316..39199
                     /locus_tag="IW774_000226"
     CDS             37316..39199
                     /locus_tag="IW774_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1152606.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39274..39558)
                     /locus_tag="IW774_000227"
     CDS             complement(39274..39558)
                     /locus_tag="IW774_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1152607.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39548..40282)
                     /locus_tag="IW774_000228"
     CDS             complement(39548..40282)
                     /locus_tag="IW774_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1152608.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40411..41034
                     /locus_tag="IW774_000229"
     CDS             40411..41034
                     /locus_tag="IW774_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1152609.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41226..42347
                     /locus_tag="IW774_000230"
     CDS             41226..42347
                     /locus_tag="IW774_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1152610.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42354..45482
                     /locus_tag="IW774_000231"
     CDS             42354..45482
                     /locus_tag="IW774_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1152611.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45501..48695
                     /locus_tag="IW774_000232"
     CDS             45501..48695
                     /locus_tag="IW774_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1152612.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48841..49548)
                     /locus_tag="IW774_000233"
     CDS             complement(48841..49548)
                     /locus_tag="IW774_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1152613.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16278 a         8792 c        10416 g        14441 t
ORIGIN      
        1 aaaaaaacat tgaaaatggt atgataaaag aaaaaaagga ggaaattttt aatggtgaaa
       61 tttatccatg cggcagattt acatatggat cggtcatttg aaggactgac aactcttgat
      121 aaaactgtac aggaaaaact attgaagacg aatctgaccg ttttatcaaa tatcattgaa
      181 caggcaataa taaataacgt tgattttgtt ttacttgcag gagataattt tcatcaaaat
      241 cgtccttcat taaaaataca gaaacatttt tcagaacaga tgaaacggtt agaaaagaac
      301 catatacctg tatttataat attcggaaat catgatttct atcaaaaaga gcggtattgg
      361 ttctcctttc ctaaaaatgt ccatttattt acatccgaaa cggtagagac aaaaaaaata
      421 acgataaaaa gtggagaaac tgtctctctc agtggattta gttatcgtca accgtggatt
      481 caaaaagaca aagtgatgga atttccttct agagaactca cagattacca tattgggttg
      541 taccacgggg aacctggcgt ctctcaaaaa ggaaactatg ctcctttcca accctcaaag
      601 atgcaagaaa agggatacga ttattgggct ttagggcata tccatgtgcc aacggtctta
      661 aatgaaaagc aaacaattgt ttatccaggt gcgcctcaag gtcatactaa aaaggaacaa
      721 gcttctacat ctattctact agttgaatta agtaaaggaa gttgccaaat acatccgatt
      781 aaagtagcag aggtctattg gaagacaaag gaaatatcat tgaggaaagc aagaacgaca
      841 aaagaggtca ttgttcacat tcgtcagcag ctttcaaaag tagaggatgg atttagttta
      901 attgagataa aattaaaaga ttatgaccat ctcaatacag atgttttgga acggatacaa
      961 tcaggagagc tattagacta tttgttagag gaattctcag ataggataaa tgatttcttt
     1021 atttggcgta tttctctcat agagaatact ttttatacga agaccccact tgctgcttct
     1081 gctaaattga tggaacagtt atttcaatac tatcagacac ctcaagattt ccaacaaatt
     1141 ttaaatgaag tatatagtca tcaagaagcg gctagaatat tgagcgaact gccggaatat
     1201 caagaagaaa cacttgaaaa agccaagcag ctgcttaatc aagatttttt atttgaggag
     1261 gatcaggaat gaagatttta aggatcgaga ttgctgcttt tggaaaatgg cgtcaaaaaa
     1321 gttttgactt ttattcaggc aatcagctga tctatggggg aaacgaagca ggaaaatcaa
     1381 cgatttatca atttattcaa gcaatattat ttggtttccc ttcaaaaggc cgtaaaaaaa
     1441 aggattatac accaaaagac ggctcagcct atggcggcaa aatatggctt aaacatcctg
     1501 tatatggtga atttgctgtc gagcgctaca agcagcaaaa tcgcggaaaa tcgaaggtgt
     1561 ggctggggga tcaagtggga agtgatgagt tactagaaaa actgatcgca cctttgacga
     1621 aagagttatt ccagcaggta tttactttcc agcaagagca gttgatggaa ctagatcatt
     1681 tgagagaaaa agagctacat gatgcgctga tttctttagg catcacgggc agttctcaag
     1741 tattccaaaa gcggcaagaa tattatcaga aagcacaaca attattcaag aaaaaaggcc
     1801 aaaaattact aatcaatcaa aaattaaatg aatggaaaga gctgcaagaa aaaattcatc
     1861 aaaaacaaga acaggaagct tattttggca agttgatcca acaagaacag gaatacgagt
     1921 cgcaacagca gcagttattg gcacaattaa aagaggcgaa caaacaattt tcattagcca
     1981 gacagcaaga acttaatttt tctttatatg aagaatggca atctctcaaa aaaaacagca
     2041 ctacaggtct tcctgatgag caacttcttt tagatctgga gtctttctcc aaacggtatc
     2101 agcaaacaaa tgagcgtttg gaagaattag aagcaacact ggaaaagcat agcgggatgg
     2161 atcagcagtc agccagatat tatttttatt tagagcagga gcaggaacta aaagatctcc
     2221 agcaacttca atttacagcg gaacaattaa acgaagaatg gcaaagatta caaattgaga
     2281 ctagagaaac aaattcagaa ttgaagattt tggaacaaag gtggcattgg cagaaaaatc
     2341 cgttaccgga agaattttta tatgaagaag aatggaagca tcttctggct gaagaaagaa
     2401 ggattgctga tctagaaagc cagatttctg tgcgtctcca aatagctaaa gaacagcaag
     2461 agatgattga agcagaagtg aatgacttgg aagagaccta tccagcatta ttacaaaatc
     2521 agtcagagat agcaccagtc tcttcatcta aaccgtatat gctgatggct ggcggagcaa
     2581 taatcattgc aggtatattt gtaccaatgc ccttgaaagt gatcctttta ctaattggtg
     2641 caggcatgat tggctatttc ttctacaaaa ggcaaccaaa aattaaaact aaaaatgatt
     2701 tctctgagat aaaaggaatg tggcaagaaa aactaggaca acttgatcta gccttagctc
     2761 aattagctga agcagaagaa aaacaaaaga gaactggaga agaaaaacag attcttcagc
     2821 aagaagtata tcgttttgcg gatcatcggc acttaggaaa gatggatacg ctcaaactca
     2881 tgaaagaata tggacaggaa gtcatagatt atcagaaatt atatggcgta tttgagcgga
     2941 aacataaaag agaacaggaa atacaagaaa aactacgaca aatcgaccaa aaatttgagt
     3001 ttgttaaaga atggttaccg atacaagata agacaatttc agaaaaaatg gtattgcttt
     3061 cccgttttat acaagagatg gaacagatga aatttgccag aagctatcag caaaatactt
     3121 tattgaagca agaaatcaat caattgaaaa agaaacaaaa agtatggata caagaataca
     3181 gcgaacaatt gactcaagca ggcttatctt atccttcgga agtacctgtc tttttacaaa
     3241 aagccaaaca agccgaacag cagatgaaaa gatacaatga attatcggaa atgcttactc
     3301 cactatttcc tgaagagctg acaaaagaga aattatctgt aagactgcgc caactgaaag
     3361 aaaagcaatc ccaactccag caagaagaaa aagaagcaag tgaaaatcgg caacgactcc
     3421 aacttcaagt agaagagttg caaagagacg gcacattgga cgaactttat caacaagcta
     3481 gccggcaaaa agcaaaatta caggaattac tggaggaatg gggagcactt gaaatcgccg
     3541 ggacattctt aggtgatctt tcaacagaga tgagtgaaag acaattaccc cagttgttaa
     3601 gagcagcagg tgattattta ggtattttaa caaaaggaaa ttatcagaag atattactga
     3661 aaaatgatac actgacttta acagacggat atacagaatt tcccatctat gagttatcta
     3721 caggtacgaa agatcagctg attatggcga tgagatttgc ttacctctca ttagaagcta
     3781 accgttcgat atgcccgatc atcattgatg atggctggct tcattatgat catcagcgaa
     3841 agtatcaatt agctgaactt ttagctaaat ttggagaaaa gtatcaaata atttgttttt
     3901 cttctgataa agaaatggta agctattacc atgagatgaa ccaaatcgta aaacaattag
     3961 aaggagcaaa taatgaaaaa aattcgtgat ttagtaatcg atgagcagtt tgaacaattt
     4021 gtcctgatca aaagtgcaga tgtaagaatc gctaaaaacg gaaaaaaatt tattgctttt
     4081 actttccaag atacttcagg tactatcgat ggtaaatact gggatgcttc ggaggaggaa
     4141 atcaataaat ttacagccgg cacagtcgtt tttctaaacg gtaaaagaga ggtttatcaa
     4201 ggaaaccctc aagtaaagat tcttcatatg cgcttaacaa gggtagatga gccaggagag
     4261 cctagtttat acatggaaag agcccctttg aagaaagaag aaatggaaga agaaatcaat
     4321 caattgttgt ttgagatcac caatgcgcat tggaatcgta ttgttcgcta tctacttaat
     4381 aaatatcaaa aacaattttt tgagtttccg gcagcaaaaa gaaaccatca tgcatttgcc
     4441 agtggattag cttatcatac tttgacaatg cttcacttag ggaaatcaat cgccgatgaa
     4501 tatcctgagc tgaatcgagc attgttgtat gctggtatca ttttgcatga tttaggaaag
     4561 gtgattgaac tttccggtcc tatgtcgaca gaatatacgc ttgcaggaaa tttgatcggt
     4621 catctggtat tgattgacga agaaataact aaagcctgtc ttgcgttgaa aatcagcgaa
     4681 gctgacgaag atgttctcgt attacgtcat atggttttag cacaccatgg cttattagaa
     4741 tatggctcac ctgtacgtcc aagaataatg gaagcagaaa tattacatca aatcgacaat
     4801 atcgatgctt cgatgcagat gatgttgact tcgattcgtc aaacagaacc aggaaaatac
     4861 accgatcgga ttttcggtat ggacaaccga agtttctatg taccaaaaga tttatagcaa
     4921 aaatttattt ttttcattca aaacaaaaaa agcgagacat aagtcttcgc tttttttgtt
     4981 ttcttttatt ttgatgaatc agttgtttct tcagttgaag attcttttgt tgaatcagtt
     5041 gattttgtgt cagaactctt tgttgaagct gtagtttcac ttgagtcttt cgtacttgat
     5101 gaagttgttg tgaatgcact caacacattt tcaaaggcat catctttgat ttttacattt
     5161 gcatctttta attcttcacc gattacttta gttgtgaatg cattgtcact tagtttagtt
     5221 tcagtagcga tatcttttag ttgatctttg tatttatcca tgtcattgcc tttattttgg
     5281 tttttcacca ttttgacaac gtagtattct gtcgcataag aagtaggatt tgttgtagta
     5341 ataacatcgg agatctcacc gtctttcaat ttgaaagctg cttcttttac ttctgcagga
     5401 atagttgttg tcgttgagtc gaatttaact ttaccgccat cttcttttgt ctctgtatca
     5461 gttgatttgt ctttggctaa tttagagaaa tcatctccat catccgctga ttttttcaca
     5521 tcttttgctt catcttcact tgataatttg atgatttgtg cttctacttc tggatggaaa
     5581 gatttccaag cagttttcaa atcgtcatct gtaatatcta cgtgagcttt caagccagct
     5641 tcaaaagcta aattgttacg aatatattct ttataagtat ctttcgtata tccagcagct
     5701 tctaattgtg attcaaaagt atcacctaat gatttcgctt gtttgtcgta ttctgcatcc
     5761 acttgcttat cggttacttt atcgccatat ttgttattga atactttata aatgatcatc
     5821 cgttgtacta aggattggtt cgatgattca agttttgctt catcatagaa atcagataca
     5881 gtgatcgttc cgcctttcat cgttgcgata tctttattag tatcgcctga acaagctgct
     5941 agagttaaaa cagccaacgc actagtagct gccaaaataa ttgatttttt tttcattaaa
     6001 atttgcactc ctgtaattag ttattatttt tatatattaa ctataccata tcccatttcc
     6061 tttaaaaacg ttgtcgtggt agtttcaaac aaaattcaaa atttcatcac attcctagga
     6121 aatacgcaaa cttttctcac ttatatagtt aaatctgttg agaaaataat cattattata
     6181 tttgttcagg atactctggc agatcagctt ttagtttttc tacttgttca ttgatttgtg
     6241 tgatacgtgg ttcggcttgg aatttaaaat cttcgatatc ttttttgatt gcttcttgga
     6301 aagcaggaat cacagattca gctgtttctt ttgtttcgat caatgcgctt ttaaaatgat
     6361 tcaggctgtt atttaaatca tttgtcagag ttgttgcttc ttccaattct tcgatcagtt
     6421 tctttcttgt ttcattcccg ctcctgggcg ccaataacag acctccgaca gtgccagcag
     6481 cagctccaaa taacagtcct ttgaaaaagt ttttaatcat cgcaggacac ctgctttacg
     6541 attgtttctt gaatagcttt tagttcttcc gatccataac tatcttgatg attgacaaag
     6601 tgaatagaaa aatcatcctc tttgctgtag cgagggacta aatggatatg tgaatgaaaa
     6661 acagattgat aggctagttc acggttgtta ttcaagatat taagaccttt catttctgga
     6721 aaagcttttt ccaatgcttg agcaatctta ggcacacgcg aaaaaacctc tcctgcaagt
     6781 tcgggatcat attcaaaaat atctgctaca tgacgtttag gcacaactaa tgtgtgtcct
     6841 ttcgttgctt gagagatgtc taaaaaagca tagaccttgt cgtcttcata tattttatag
     6901 cttgggattt cttgattgat gattttgcaa aaaatacagt tttccatata aaacatcctt
     6961 tctataaaga ataaatactt cagcttaact ttaccataag ttaaagctat tgtaaggaaa
     7021 aaaacaaaat accgtcattt tcttcgtggt ataataaata aaaataaatg gcggaggaaa
     7081 aagaatgagt ttaattatcg atcatgtcac aggggggtac ggacatctcc ctgtgttaaa
     7141 agatatcagt ttcgatgtac agcctggaga gatagtaggg ttgattggac tgaacggagc
     7201 agggaaaagt acgaccatca agcacattat cggtctgctg gaagcttcaa aaggaacgat
     7261 agaaatcaat catctatcct taaagaaaaa tacggaagaa tatcgcaaac aaattgggta
     7321 tattccagag actccttctt tatatgaaga attgacattg agagaacata tcgaagtcac
     7381 tgcgatggcc tacgatatac ctatggatat agcaatgaaa agagcagaaa agttgttgga
     7441 gacttttcgg ttatctaatc gtcttgactg gttcccagct aatttttcaa agggaatgaa
     7501 acagaaagtg atgatcttat gcgccttttt gatcgagcct tctttatata tcatcgacga
     7561 accattttta ggcttggatc cactagctat caatgcattg cttgaattga tggtagaaat
     7621 gaaaaaagaa ggagccggta ttttgatgtc tacgcatatt ttagctactg cagaaaaata
     7681 ttgtgatcgt tttgtggtct tacataacgg agagatacga gcagacggtt ctcttgagat
     7741 gttgagaaaa gaatttcagc tgccggattc ttctttggac gaaatctata ttgctttaac
     7801 aaaggagcag gcacatgagt agtttttttc aaaaacgttt agcaagacat caaaagaaaa
     7861 tgatgaaata tatgcgttat gtattgaacg atcattttgt tttagtttgt cttttcttga
     7921 ttggcggtgt gggattatat tattctaatt ggttgaaaac cctgcagcca ccgtttgcaa
     7981 ttggagggct cataatctct gtattttgga tgctctgttt attcgtgggt aagatggcta
     8041 cttttgcaga ggcagcagat attgtttttt tacttcctaa agaaaaagaa atgcaaacct
     8101 atttggaacg agcttttcgc tattcttgca tttttccgtt gatctttttg atattggcat
     8161 gcggattggc catgccatta gtggtggtta gtacggagca atccttttcg atgtttttcg
     8221 tctatctgat catattatgg agtttgaaat tcagtcatct gcaaatcaag agagcagaat
     8281 tgtttcgact agatcaacaa gttattcgtt attggaaatt aggctggttt ttttcttctc
     8341 tgatcttttt gtttctatca ttttattggc taatatgggc aggaatagca ggtgcattag
     8401 tacaggcaag tctatattac tggtttttgt ggaagcaaca agaaaaagga ttggattggg
     8461 agaaaatggt gcatgtagaa gaacataggt tgcatcagat ctatcaattt atcaatttat
     8521 tcacagatgt tcctgaaatt acggcaaaga tcaaaagaag acgctatttg gatcctatat
     8581 tgactcatat taaaagagaa tcaaaaaaca cttatcttta cttgtttgca agaagattgg
     8641 caagaggttc tgattttagt ggtctttatt tccgattagt aattataggt gggttattga
     8701 ttgccggagt agtcgacttt tattttagtc tgattatcgg tttcttattt ttatatttga
     8761 tcggtttcca actgttacct ttagcgaacc agttccgcta tatggttctc gttcagcttt
     8821 atccaatcca taagaatcag aagaagcagg cgattcagta tctactcaca tgggtattaa
     8881 ttatcgcgtc ggttattttt ggtttgattg ctgcagtcgt attagatggg cagcagcgat
     8941 gggttccgct agcggcttat ttgattgtta ctatgctctt tgttaaacta tatgtaccaa
     9001 ctcgtttgaa gaagatgtca gactgaaaac aaagctgtgt agggtaaaaa tgagaatgaa
     9061 aaatagagcg ttgacagtaa gaatgctgtt cgttctattt tttttgagaa aagctggttt
     9121 tttgaatggc aattaaatga aaaagaacgt atatgtgctt gacgtgtagt gttcaggcga
     9181 ctagaataat aagtactgca aaacaaagag taagaggacg ggagaatgca tgatggagtt
     9241 tcagttagac caagagtggc ggttacgtcc catcaaaggc gacacaggaa aaacatacat
     9301 aggcatgcgt gatgaggaca aagtctttat taaacgaaac acgactccga tgttggctgc
     9361 tctttcaagg gaaggaatta caccaaaact tgtttggaca aaacgaacgg ggaatggcga
     9421 tactttgaca gcacaagagt ggctggaagg tcgcttgctg actcctaatg agattggtca
     9481 aagaaacgac gtgattgatg ttttgtacca tttacatcat tcaaattctc ttaaaaccat
     9541 gctgacccgt atcggtggtc gtgtcatgac gccggagaag atgctgcaaa aatatgaaaa
     9601 agaattaccg gaagaactcc agaaaaatca atttctgcaa attgtccatc gttttcttcg
     9661 tgagcagatc ccgtcaatag ataaggaaaa cttatcagta gtacacggag atgtaaacca
     9721 tcgtaattgg ttagtatgta aaaattattt atatctagtc gattgggatt caatcatgtt
     9781 tgcagaccct gcggtggata ttgcaacgat tttaggaaat tatgtgccac tgtctagctg
     9841 gaatcagtgg ctgatcagtt atggcatacg cccgactgac gaaatgcttg aaaaatttca
     9901 ttggtatgcg gccatgaatt tgctccaaga aatcaccaga tattgcaaac gtgaagatca
     9961 tcgacggatg aacgaagaaa ttttacaatt aaaacgaatt ttcagtggct gaggtttggc
    10021 ccaaactctg cggagatttg gccagacctt tttaattaga ggaaaggaat ggagataaat
    10081 aatgcgtgtc cgaaatcgtc cgggagcagc ggaaatgtta gcggctcacc cgaattttgt
    10141 aattagtgat ccaacattgt ggaagggaaa atggaatgaa ttgtttgaaa acgatcatcc
    10201 aatccacatt gagattggta tggggaaagg ccagttcatc actggaatgg caaaagccca
    10261 tcctgaaatc aattatattg gagtagaaat gcaggtcagt gttgtttcga ttgcactaga
    10321 caagttgatc gaacaaccgc ttcctaacct gaaacttcta catgtggatg gatcagcgtt
    10381 gacagagtat ttcgctgaca gcgaagtaga tcaaatctat ctgaattttt ccgatccatg
    10441 gccaaaaaaa cgtcatgaaa aacggcgatt gacatataag acttttcttg ctgtagatga
    10501 acagattctt cgtccaaatg gagaaatcca tttcaaaaca gataatcaag gattgtttga
    10561 gtattctctg gcaagttttt ctcaatatgg catgatcctc aaacaggtat ggttagattt
    10621 acatcaaagt caatttgaag gcaatattat gacggagtat gaagaaaaat tttcttcaaa
    10681 aggtcaacgt atttatcgag tagaagctca atttcaagat aagaccaaaa agaaaagtct
    10741 gtgaaactaa ctttctgctt tcataaaaac attaatatcc ataaataaat cccagaaaat
    10801 gaacattaca gatgtaatgt attttctggg atttatttga tttaaaatgt atttatttca
    10861 tcggtttttc cacaaggtgg aatcaattgg aagcgagaaa atgaaaataa tcctttgtcg
    10921 gtgatcttca attcgggtat gacagataat gttaagaaag aaagtgtcaa aaaaggatca
    10981 aaggttttag ctgctcctag ttgatgtgct ttagttatta attcattcaa ctgttcatag
    11041 acgatcgaaa aaggttcctg tgtcatgatt ccgcctatag gaagagaaac acaagctatt
    11101 tcctgctgtt tgttgacaac ggtcatcccg ccaccttttt gaatcaaaag attagcagca
    11161 tacagcatat cctcgtcgtt tgtcccaacg acaactagat tatgcgagtc atgggcaatc
    11221 gttgtcgcta tcgctccttg tttcagctga aatcctttca ctatgcctaa tccaatgttt
    11281 cccgtttgat ggtgacgctc gatcaccgca atctttaatt ggtctttttc gattgaagga
    11341 atgaatcgtt cgccttgtcg gtccactttt tctaccaaat ctttagtcac tagactattc
    11401 gggataattt cgatcacatg ggcttgtgaa gaagaaagaa gcagctctaa cgaatgtgga
    11461 tttaatggtt gagcgtttag ttttggcaga caacacgtat atgaatttga atgatcagca
    11521 aataaatggt cttggagttg cccagattga acaactaatg ttcccttata gaaaacttca
    11581 tgaattggca gcttggtaag atcgtctgtt aagaagaaat cagcttgata cccaggggca
    11641 atcgcaccta gtgttttcaa atcaaaacat tctgctgcat tcaatgttgc catctgaatg
    11701 gcttgtaatg gatcgattcc aagtgcaata gcttgacgga cgatatgatc gattgatcct
    11761 tcttttacta aatcattgat cagtttatca tcggtgacaa agaagcatct tcttgaattt
    11821 tcaggtgtga tcgccggtag tagcgatgcc agatcttttg caacggtccc ttctcgaacc
    11881 attaaataca taccaagatc caaacgttct ttagcttctt ttgcagttgt agcttcatga
    11941 tccgtccgaa tacctgctgc aggataaaca tttaactctt ccatgccgat accagctgca
    12001 tgcccgtcaa tttttttctt atgctggtgc atcaattgga tcttttctag gatgtctgtt
    12061 tcattatttg ctactgcttg atagttcatg acttcagcta agcctaaaac ttcgggtttt
    12121 tgtaaaaaag gggctaattt ttttgcattt aaatttgcac cattggtctc aaaaggggtt
    12181 acaggtacac aagaaggtag cataaaaaag atattcatga gtgtttgtct tgcatcttct
    12241 atcataaact caagacctgt actcccagca atgtttccta tttcatgggg atctgtcact
    12301 acagtagtaa ctccatgaag caaggatact tttgaaaatt cgcttggtga taaaagtgag
    12361 ctttcgatat gcacatggcc atcgatgaat cctggcacta gatattgccc attagcatta
    12421 tgtacgcttt ttccttcgta ttcaccgatt cctacgatca ttccttcgca gattgctaca
    12481 tctttttcca tgatttcttt cgtaaagacg ttgacgatct tagcatgctt gatcactagg
    12541 tcagcaggct tttttttact tgctacttcg atatatttcc ttaatgttgt ttccaatgga
    12601 gtgcctccca agataaattt ttataagtat aacataaatt cggtttttgc caatattttt
    12661 ttgtaaattc attttatttg aaacaaaaag atcaaaaaga cgtatgtttt ttgtggaggg
    12721 gagaataatg gaaataaaac gattatcgga tcaaatgata agtgaaagtt ttgccaaaca
    12781 tgcccaagac gtatatatat tgccagcttc agaagtattt tacatctatt ttcgctatgg
    12841 aaaaaaacga acattctgga agaaagtctc ttcggcagat ggacagcaac tgatctcaag
    12901 atttaagtat ttgggacaga tggatatcgg agaaaaaaga aaagctcagt taggtgcatt
    12961 tacttattct ttagaaaaac aggaagttcg tctgcggttg tcttctgtag gagattacct
    13021 tcaacgagag agtttagtga ttcggctatt atacggtact tttgaaacac cccatcgttg
    13081 ctttagttct cgagattttg caactatttt acagaaaacc gtcagacgtg gattatacct
    13141 tttcagcgga ccagtcggat caggcaagac ttctctaatg tatcggttgg ctttggaaga
    13201 aaaattgcaa gtgattacta tcgaagatcc tgtagagata gaagaaccgt tttttctgca
    13261 gctacagatc aatccgaaaa tagcacagac ttatgataca cttttgagac tcgcattacg
    13321 gcatcgtcca gacttgctaa tcattggcga gataagagat gaatcaactg cacatgctgc
    13381 ggtaagagca gcactaacag ggcaccgggt ttttgcaacc gtacatgcac gcaatcttga
    13441 aggtacattg gtcagaatcc atgaactgac aaagaaaaaa gaagagctat ctgaatgttt
    13501 agctggtgtc atttatcaag aacttttgga agatgccaat caccatcctt ctgttttatg
    13561 gagttatcaa tttatggagg aagaatcagt aaaaaaatca tgggaggaaa gttatgaaga
    13621 agctcaggaa aatatttggc agtcagaact attctaaagg attaacaaga agccagcaaa
    13681 accagtttgc acaaaccatg ggagaactgc ttttatctgg ttttagtctg caagaatccc
    13741 tttatttatt actcacgatc cgttcgtttc cacctgttat tttggcaaat atccaacatc
    13801 agctaaagga agggcaagcg tttagtgagg tactttcgca aataggatat ggagcagagc
    13861 aactggtaca gatcgagtta gctgaacgtc atgggaatct gccgacaact ttgttggaaa
    13921 ttgccaaaca gtttcggctt gttgaaggat ttcgcaatga tttgaaaaag ctgcttgcat
    13981 atccgatgct attgctcacc tttttgctag gtatcttgtt tagtatgcgg ttgttcgttt
    14041 tgcctcagct tattggttca ggaatggtca tgcaagagca ttggggtatt cagctgatac
    14101 aagtctttca ttggtatgtt ctactattca tcctagcggt tactctattg gctttactag
    14161 taaggtatcg tctgatgaag ttgtctcctg ttgatcagag tgaatggata agcagacaat
    14221 tattgattgg accgatatat tccttgtatc aatcggctta tttgtcacta gagattggaa
    14281 agctttttca tgaaggtttc gaattaaggc aaatcatagt ctgcttaaag gaaacgaaac
    14341 aagaaagtct gattcagcat cttgcctcta agttgattca aggattggag gcaggtatgt
    14401 cattggctga gcagttccag aattatcgtt ttctatcaga agaattcagt aaaattgttt
    14461 tacagggaga ggcaaaggga agtttaggta aagaactgat tttttacagc gaatggacaa
    14521 gaaaacagct tttcaaacgt cttcatcact ggatgcattt tattcagcca atcatttttt
    14581 tgggcgtagc ggctttgatt ttattagttt atgccgctgt tttattacct gtttacggaa
    14641 gcatagagga ggttttgcca tgaaaaagag aaaaatatct tattatagcg gttttacatt
    14701 gatcgaaatg ctaatcgtct tactgatcat cagtgtatta gtgttgttgt tcgtccctaa
    14761 tttgtcgcgt tataggaatc atgtagacca agaaagtcgt gaagcgatca tccagttggt
    14821 agacacacaa aaagagctct atgcgcttca aaataatgga agagttccta ctgtggagga
    14881 attgttgaat gaaggctata tcaaacgaga acatgcagaa atctatcagc ggccttgaag
    14941 gtaaggtttt agcatcagga tatacgttgt ttgaatcact cttactgatt ttattactta
    15001 caacgatcct ttcttttcca gttctcgcat tttctgcatg gaagaatgaa ttggcagtct
    15061 atcaattttt cggccaattt gaaaaacgaa tctattctac gcaaaaaatc gctattgtga
    15121 atcagatcca aacaggcttt tattggaata gtgaagagaa tcaaatcatt tttcatgttc
    15181 cgaatccaga taaaataaat tgggaagtat tagatattcc tgatgagatt accttgaaac
    15241 gccatgcttc tattactttt gctgctagaa ccgggaatga aagttcacta aaagcttatc
    15301 aattttattg ggaagaaaaa gagcggacga ttacgtatca attccagata gggagtggtc
    15361 gctatacaaa aagaattgaa taaaacagga tttgttttgt tggagtccct tacagcgctt
    15421 atgatcgctt ctgtctgtgt ctacttagtg agctttggcc agctgttttt gcttcaggca
    15481 gaaaagaaaa gtggggagga actgctattt attcgaatga tttatgaaga tatcaagacg
    15541 cagcggatgt atcacaaaag accagagcga agggaagaaa tgatcccata cgatatccaa
    15601 attgtcgata ataatggagt aatcgaaaaa gtgtttgtca atatggagaa agaagcgatc
    15661 agtattgaaa aaacaagtaa ttccagcatt tacgctgatt gaatgtctaa ttggactgat
    15721 gatattgagc agtctattgc tcacattcaa tcttttgatt cagcaaacca tccaagtaag
    15781 tgaatttttg cagcgtaaag atcaaaaaga gtggctaata tttttagcac aattagaaga
    15841 agaactcaag agcagtcaca atgtctctgt gaaaaatcag caactgcact acacgatcga
    15901 ggataaacag tatatcatcg aaagatatca ggcaatgata cgtaaaagaa gaacttccgg
    15961 cggacatcaa cctatgttaa caagtatcac tgagcttcaa ttattggaaa aagaacacac
    16021 aatttctttt tatgtacact ttgaaaatgg agaagatgga tatgccatat ggacaaaaaa
    16081 cgatcagtag aaaatgttat aagggctcta ttttattttc tgtcttgctt ttatttcttt
    16141 tatgtacgtt tttattgatg gcagcattag aaagctaccg tttgtcaatg gatcttaata
    16201 tacgaatcaa aaattattat atagcaaaaa taatgaagca gatggcttta tcggaacagt
    16261 ctacgcttgc cgaaagttcg gaaggagttt tttactacac aactggatct gtaacatatc
    16321 agtgggtaca acagtcccta ttcttagagg tggaagtctc accgtttatc tttcggttca
    16381 cagaagaagt aaaaaatgat acagatacta gtacagaatg atggaatgtt cgtgaaaacg
    16441 ccctcttttc tgttataaca ctgtttttaa taaaaaaatg tttgccgttt cacaaataaa
    16501 aaacagtgaa aacgattgaa attttgtcaa ttgttcgata gaattaaaca ggtgaaaaaa
    16561 ttgttataat atatctagaa atgaggtgca ccatgttacc agaaaaaatg gaacaagggt
    16621 ttaatcaaaa cctggaagct attcagcttt tacagaatgc attgggaact tcctttttag
    16681 aagcctatgt cgaaaatgca gaaaatttaa tagatgatta tcaagtacgt gtggtggatg
    16741 gagtgccgac aaaagagaca acgcaacgga tcacagctct ttatgaagag ttgaagaaac
    16801 tttctttcga gccagaagaa tggcgcagac tgtctcaatt acttttattg aagggcagcc
    16861 agacagaaca tttgcaggcg aaccatcaac tcactcctga tagtatcggt tttttatttg
    16921 tttttcttat tgaacaacta tacacaaata aaaaagcacc ggttaagatt ctggatattg
    16981 cggcaggaat gggcaacttg ttattaacag ttcttttaaa cctatcgaat gcggggtacc
    17041 aaacagaagg aattggtgtg gatattgatg atacattgtt ggctgtcgca gcttctacaa
    17101 gtgatttgac acaagcaaat gttcaatact ttcatcaaga cgggttgcag gaattattaa
    17161 tagatcctgt cgattttgca atcagcgatt tgccaatcgg ttattatccg aatgacgaaa
    17221 aagcaaaaga gtttttaaca agtaccgaag aaggccatag ttatgcacat catttattgc
    17281 tagagcaatc aatgaaatat gtgaagccgg atggttttgg tttgtttttg atgccgagtg
    17341 gatttttaga aacggatcaa agtgaagaaa tcaaaaaatg gtttaaagaa gaaggttatc
    17401 ttcaaggaat gattcaattg ccagatgaat tgttccgtaa taaacaatcg caaaaaagta
    17461 ttttgatctt acagaaaaaa ggaccacaag cacaacaagt aaaagaagtc ttgcttgtga
    17521 aacttgcttc attaaaagaa ccagagaagg tgacggaatt tttcaacgag tttaaaaact
    17581 ggaaatcatc tgctctgtaa tcattcaacg agaaggagag tcatatgtct aaaacaattg
    17641 caatcaatgc tgggagttca agtttgaaat ggcaattata tcaaatgcca gaagaaaccg
    17701 tagttgcgaa agggatcgtc gaacgtatcg gtttacaaga ttctattttt accatcaagt
    17761 acggcgataa tcaaaaattc gaacaaatca tggatatcga aaaccacgaa gtagcagtaa
    17821 aaatgctgtt agatcagttg attgaactgc aaattttaag ctcttatgat gaaatcaccg
    17881 gagtagggca ccgtgttgtt cacggaggag aaacttatgg ggattcagta gtgatcgatg
    17941 agaaagtaat ggaccgcatt gctgaattag cagaatttgc tcctttgcat aatcctgcaa
    18001 acttaatggg gatcaaagcg tttaaaaaga ttttacctga cattttgagt gtggctgtat
    18061 tcgatacatc tttccactca acaatgccaa agcaaaatta tttatatagc ttaccggtgt
    18121 cttattatga agatttcaaa gtaagaaaat atggcttcca tggcacaagt catcgttatg
    18181 tgtcggaacg tgcggcaaaa ttattgaaca aaccgatcga agaactaaaa atcatcactt
    18241 gccacttagg aaatggtgta tcgatcacag cagtcgatgg aggtaaatcc atggatactt
    18301 ctatgggatt cacaccatta gctggggtga caatgggtac acgttcaggt gatatcgatc
    18361 cagctgttct cccatattta atggaaaaat tagagattga tattgatgaa atgatcaatg
    18421 ttttgaacaa gaaatctggt ctgctaggcc taacaggatt gtctagtgat atgcgtgatc
    18481 ttgaaaagaa ctatgaaaaa gaacatattc gtttagctta tgacattttc gtggaccgca
    18541 tccgaaaata catcggcggt tatgtgacaa cgatgaatgg tgttgatgca attgtcttta
    18601 ctgctggtat cggcgagaat gacggacatg tacgtagtga aatcatcaaa ggtatgtctt
    18661 ggtttggttg tgaaatcgat ccagcgttga atgaattgcg tggtgaagaa gtagatatct
    18721 ctacaaaaga atcaaaagta agagtattag tgatcccaac agatgaggaa ctaatgattg
    18781 cccgtgatgt ggagcgtctg cgcggctaag ccaaagcatt cgttaggata aaagatgaaa
    18841 cgcatgcaag agggaattct tgcatgtgtt ttttttgcgg agaagaccgc aaaatggtaa
    18901 tttaacttca atttcccttc ttttttacag gatcttctta gggaattttg cttggaaaag
    18961 tggtcaaaag ggaaaagact ggcattttat ttattgattt tcaggaaaat cccaatattt
    19021 tattttcatt catctttata agggacatta catatgtaaa aagaaaaaac atttttagat
    19081 cagcctcgtg ggctgtcgac tttattcttc gctttatgtt tagctagaaa accgatccga
    19141 aacattcatt gatcgaataa taaaaacttg attttccacc tgtactttgc ctgaatcgtg
    19201 aaagcacagg tatgaaaatc aagttttttg ttgcgaaaaa acgtcctagt caatcagttg
    19261 gccgagcggc tggcggctga tctgctcctg atcaataacg agtgcttctt ggaagtgttg
    19321 ctgcaaaaag tagcggtcat ggctgactgc taggagcgtt ccttgatagt cttgcagcat
    19381 ttcttcaatt tcttctcgag catagctgtc gaggtgattg gttggttcat ccaaaatcag
    19441 gacgttgatt ttttctaaaa agagtttcag caacgccaaa cggatttttt caccaccgga
    19501 taaatctttg atccgtttac ccacatcgct ttgataaaag ccacttttgg ctaattgccg
    19561 tcgagcgact tcttcgtttc cagtcaatgt gatggcgtaa gccagcaaac ggctatcggg
    19621 atcggcaaac tgcagctgct gcggcaaata accgatttgc aagctactac ctaatttgat
    19681 ggtgccaccg taaggctttt cctcaccgag taataatttg agtaaggttg attttcctgc
    19741 accattgtcg ccgatgatgg ctaaatgatc tccacgatag actgcaaaac tgctgtcttt
    19801 gaatagtact tgatcagcgt actgctggtg caagttttga atgatgaaaa cttctttgcc
    19861 agattttcca ccattagaca agctttgcag ccgatttttc gggggaggtg gcggtttgac
    19921 gagtgtcagt ttcgccaggc ggcgttccag ttctttggct tttttgaaga aggactcatt
    19981 gtctccttca tgtgcccact gacgaaaacg acgaatcatg actttcaacc gctggacttc
    20041 tttttgctgc agttcgtaat tttttgtcag ctcagccaag cgagcttctt tcaagactcg
    20101 aaaacggctg tagtttccgg gatactcaat caatgcacct tcttccagtt cccatgtttt
    20161 tgtggtgact tgatcgagaa attgccgatc atgagaaacg accagaaccg atttttttgt
    20221 attctgtaaa tagttttcca accagcgaat actgttaaga tctaaatgat tggtcggttc
    20281 atcgagtaaa agtaaatcgg catcggctgc taaaattttg gctaaagcga ctttaactcg
    20341 ttcgcctccg ctaagttctg ttaaccgtgc ttcctgacgg tgtggaatcc ctaagccttg
    20401 caagatcgct gcaatccggt cttctaatac gtaaccgcct agttcttcaa agcgatgctg
    20461 cagctcaccg taacgggcca gacacttctc taagtttgta tctggttctg tcatcaatgc
    20521 ttcgtagcga ctcatttgct gtttgaggtc ctgtatctca gcggcagaat ggagcaagta
    20581 gtggttgacg gtcacttcgg aaactgtaag ttcttgttcg accgtcccga tctttaagcc
    20641 ttttttgcga ctgataacac cgctgtcgac accttctctg ccggtcaaga tttgtaaaaa
    20701 ggttgatttg ccagttccgt tgataccgac taaggcaatt ttttcaccag cttcaatttg
    20761 acaggataaa tcctcaaata atggattccc gccgattgtt tttttaatag aaattgcttg
    20821 tatgatcatg atctctctcc ttgagaccac tcaaaaaggc catggttatc catggcctgt
    20881 gttatttaaa ctcagattat catgagaaaa ataccttgag atggccattg attttacgtg
    20941 aattcaactg ttttggacaa acttgtcccg tcaacagcaa ctgtaaaacc aattgcatgg
    21001 ctaaatgcga tatggtatcg ttggtaattt tcattacttg ttattttagt catgcgcatc
    21061 atagtatttt ccctccttcg tgtagttgtc ttttccagtg tacctgaaag tgtcagaaag
    21121 ttcaactagc gctgacattt acttaatatt tatcgatgtg taattctgtc agagagcttt
    21181 ttttatctga ctgagggtaa tgtctcgtgg accacatcat tgtattctga gctaaaccag
    21241 cgttcaggcg aaatcatatg gtgtttgata aaaaactttg tctacatgac ttccttttgc
    21301 catttgctta ataccgttgc attaaacagc tcttctcttt ggaaatctca ccaacatcgc
    21361 gttcaggagt atcgttgtgg ttgcaatggc aagcggtagc ctttcatcaa ttcttgaatg
    21421 atttaccgaa tgtagcactt gccttctcgc ggtacccgaa cccgaaataa aattttagtt
    21481 tggcgataaa atcggtatcc gccatcgata cttcatattt tttcgtatca gattcatcgg
    21541 aaacttccac attgacgaac caataagctt tggcatgctt aggtcgtttt aaaccacacc
    21601 agggcgacta atcgccctgg tgttcacttt tttttatttt acttgaactc attgactgtt
    21661 ttccctttat tttaaacatt ataaagaccg atgagtgctt gccgcgtctt ttgtttttat
    21721 cacggttttt tgtgcaaaaa aacctgccga tagttggcag gtcgtctacg tgttaagctt
    21781 tggtgtggtc tttagctggc aattggtttt ctaaatcagt tcggacaaca agcacatcgc
    21841 aagctgcgtt tctaataaca tattcagaaa cagaaccaat aaataaacgt tctacagcat
    21901 tcaatcctgt ggctcctaac ataatcaaat ctacttgatt atcttctggg atttctctgg
    21961 caataatttg ttttggtgag ccatattcta cgacagaggt tacattattc aagccagctt
    22021 tcttcgcatt gctttcataa tctgccaatg tttgctttgc catttcagta gcttgttctg
    22081 caagcattcc atcaaaggat gaaacggttt ggaaagaacg tgtgtcaatg acatgtgcta
    22141 atagaagttc tccgttattt cggatagcta cattgaccgc ctttttaaaa gctaattcag
    22201 cttcatcaga accatctacg gcaaccatga tttttttgta ttgttgtaac ataagcattc
    22261 actcctcaag tcaatcgtat tgtactcttt tattgtagaa cactttaggg aaaaagagaa
    22321 ttatttactg ctttggttca gcaatttttt ttgcaaatcg gatactgaag gtggcagaaa
    22381 caataattac gatcagtaaa aagaccaagg caataaagcg ttgattgaaa aaagctgtta
    22441 aaatacccat tacgcctaaa acagcatatg cagctccgta aaattgtagg aatccttgat
    22501 ttttatcatt tttgttgatc aatacaaaaa agatatcctg tttattaaat aaataatagc
    22561 taacgaataa taatactaaa gtggcgataa tcattaaaat cctaatcatg aacaaatcct
    22621 ccaaaataag caacaaatag aaaaagcaat ttgccaagag caaattgctt tattctttaa
    22681 aagatggtaa tctccgttaa tgccgttgtt tgtcctgtag tattgatccc gcgaattcaa
    22741 gcaggtgggt cccatggtcg tagcttcgaa ctaccaaatg ataaggcatg ttaccagcag
    22801 tgacacaaga cgggtcccaa gatatcaata aaaatgttcg gtcaacacac aaagaggaca
    22861 attcgaacat cacagatttc ccacctctat agtagcatat gaatttaaaa agagcaatga
    22921 gagagctcgc agaaacagaa ccgttttctt gtctagtttt ttggaggatg ctttttccac
    22981 tcttttatac ggtaatactg ttgacccaga gcctgttcat atttccctgt atcttttggc
    23041 tgataatact gagcactctt cagtttatct ggtaaatatt gctgattgac ccaagcctga
    23101 tcaaaatgat gaggatactg ataacccacg ccacggttga gtgatttcgc acctttgtaa
    23161 tggctatctc tcaaatgatc aggaacatct ccagctttgc cctctcgaat atcagcaagt
    23221 gctgcatcta atgccatgta ggcagaattg gattttggcg acaagcaaag atcgacgacg
    23281 acgtctgcta gtgggatacg tgcctcaggc aatcccaatt tttcagcagc taatacagcg
    23341 ttcactgtac gagccgcagc agcgggatta cctaaaccta tatcttcata accgataacc
    23401 atcagacgcc ggcaaatact tgccagatca cctgcctcaa ctaaacgggc gagataatga
    23461 agtgcagcat ccacatcact tccacgaatc gatttttgaa aagccgaaat aacgtcataa
    23521 tgagcatctc catttttgtc atgggtaaga gcttttcttt ggacacattc ttcaatgatc
    23581 gacagtgtaa gatgaatcct tccttcttta tctgaaggag tcgatagtgt agccagttcc
    23641 agcccattca atgcactccg caaatcgccg ttcgttgcac gcgaaaggtg aagaagagca
    23701 tcttcgtcta actggattgc ttgttgtcca aggccgcgtt ctttatccct taaagcatgt
    23761 tcgactgcca actggatgtc ttgttctgtt aatggtttga cttcaaagat ctgtgtacgg
    23821 ctgcggatcg ctggattgat ggtaatataa ggattctcag tagtcgctcc gatcaagatg
    23881 attcggccac tttctaaatg aggaagcaga aaatcctgtt ttgttttatc taatcggtgt
    23941 acttcatcca ataagaggat cactgtacca ctcatttttg cttcttccgc tacaacttgt
    24001 agatcctttt ttgaatctgt agcggcgttt agcatgcgaa atgcataatt agtagagccg
    24061 gcgatcgcac ttgcgatact cgtttttcca gtaccaggag gtccatatag aatcatggaa
    24121 gagagcatgc gagcatcgac catgcggcgt atgatttttc cttccccgac tagatgctgc
    24181 tgaccaacga cttcatcgat cgttcgaggt ctcatgcgat aagccaatgg ctgttgcata
    24241 ggattttcct ccttcttgtt tttacgtccc ttaattatat catatttagc aagatgccta
    24301 gaaagaacag ccttctgttg ttttgttttc atagcttatc caactaatat tgtgtatgat
    24361 aaagtaggtg agaaaaatga cggataaaca aaagatagat gaattttttt ggaataaaac
    24421 gacgccagaa gtggcacggg atttattggg gatgtatttg gagcatgaca caccagaagg
    24481 aagattagct ggatatattg tagacgcgga agcataccta ggtccagaag acgaggccgc
    24541 ccatagctat ggtttaagaa gaacaccgcg tgtgcgggca atgtatgaaa aaccaggtac
    24601 gatctacctt tacacgatgc atactcatcg gattttgaac atcattactc agccagaagg
    24661 gattcctcaa ggagtaatga tacgtgcaat cgaacccgcc gcaatgattg atcaaatgag
    24721 taaaaatcgt ggcggaaaaa ctggtccaga tatcagcaac ggtccgggaa aattagtgga
    24781 agcattagca attcctcaag aactttatgg tcagtctata gcagatagtt cgttacgctt
    24841 agtttttgag aaaaaaaaga caccaaaaaa gatcaatgct ttgcctagaa tcggtatccc
    24901 aaacaaaggt gtatggacag aaaagccgtt gcgttttgtt gtgtcgggaa atccttatat
    24961 ctctttacaa agaaaaaacc aaatcgagaa agattggggt tggaggaaag aaaatgaaaa
    25021 agaaggatct attaacatat ttagatgaaa taattgaaaa acaagtgaca gactatgatg
    25081 tagctttaga ttgggataca aaaaatcata cgatcgagat cgttgttcgc ttgtttgcag
    25141 aaaataaagc acaatttgag attgatgatg cagaagggat cgtttcgcaa gaaccaatta
    25201 tcgaatttga agacggcgta cttcttttta atcctcagaa atctgttttt gatgaacgca
    25261 attatctagc agtaatccct tatgaaggaa aaaaagggtt ggcaaaatca gtagctgatt
    25321 ctttagtgga atatctaaat gaagtattag ctcaagggca aagcgatttg cttgattttt
    25381 tggatgaaga cgatgacgaa gctgtattcg agcttcactg ggataatcag caactagcag
    25441 aattagtaga agtaaaacag caaaatgaag cagatactta tttaccgtac ccaagttatt
    25501 aaggagtgac agatatgaaa tggaatgaag taaaagtaga aactgaaagt gaagcagtcg
    25561 aagcagttgc taatattttg atggaggcag gggcgagtgg tgtcgcgatt gaagattctt
    25621 tagatgttga acattttcag tcggatcaat atggtgagat tttagataaa aatgatttta
    25681 catcgctgaa agaaggcgca ctagtcatgg cttattttcc ggaaacgact ttcttaccgg
    25741 aaatcttgcc ttttatcaaa gatcgtatca cacaattacc tgagtttggt ctagctattg
    25801 gaaaaaataa agtaacagtt agtgaagtag aggaaagcaa ctgggcaaca gcctggaaga
    25861 aatactatca tccggtgcgt attacgcgaa tgctgacggt tgcgccaagc tgggaaaact
    25921 accagacaga agatcctcta gaaaaaatta tcacactaga tcctggtatg gcatttggca
    25981 caggcactca tcccacgact tccttgacat tgacagcact agaaacagtc ttgcgtggtg
    26041 gtgaaacatt gctagatgtg ggaactgggt cgggcgtgct aagtattgca gcaaaatatc
    26101 ttggtgcaaa agatgtctat gcctttgatt tagacgaagt agccgttcgt tcggcgaaag
    26161 aaaatatgga tatgaatgaa atagccaaag atgtccatgt atcagctaac gatcttttga
    26221 caggaatcga gattgaaagt gatgtaatcg ttgcgaacat tttagcagat atcatcttat
    26281 tgatgatccc agatgcttgg cgtttactga agcaaacagg cacgctgatc gtttcaggaa
    26341 tcattgaaga gaagaagcaa atggttctgg atgccatgtt agaacaaggg tttatcgtgg
    26401 atcaaatttt ccagcaaaaa gactggtatg caattatttt gaaaaaacca gaggtggaat
    26461 agatgcagcg gtatttttta aaagaatcat atgaatcaaa agactatttt gttttagaag
    26521 gggaaaacta tcaccacatt gtacgggtta tgaggatgga acccgaggat gaagtttttt
    26581 tggcattcaa tgatcaaata gccattgttg cagaaatcac ggagattact gaagaggctg
    26641 tttatctgaa agaaattaaa aaagaagaaa atcaaaagga actgccaagt tcgattacca
    26701 tagcgagtgg ctatcctaaa ggggaaaagc tggattggat cgttcagaaa tccactgaat
    26761 taggagcgca agcttttctt ggtttcccgg catcagcctc tgtcgttaaa tgggatgcaa
    26821 aaaaacgtat gaaacgacaa caacggcttg aaaagattgc tcaagaggca gcggaacaat
    26881 cccatcggca gatcgtgcca tcagtagagt tattggaaac gaaacaagat ttatttgagc
    26941 ggctagatga ttatgacgtg gtcttggttg cttatgagga atcagcaaag caaggcgaaa
    27001 aatcaaatgc agccaaaatt cttagtgaaa caaaaccaga tagtcgaatc ttagctgtct
    27061 ttggaccaga aggtggatta tcccgcgatg aagtcgatta ttttgtagaa cacaaaggga
    27121 tactttgtgg attaggacca agaatccttc gtacagaaac agcacctctt tatttattaa
    27181 gtgctgctag ctatcaatgg gaattaatga atccttcttt cagctaacgt tcttcattaa
    27241 taaaagagta tcgacagaaa gaattgaaaa aatagtcaac aatttgtata atagaaacaa
    27301 ttatttatac aatggatacg aaaaatcgtt tgaaaaagct tttaaaaagc gaacatttcc
    27361 gtaaaaaaac gtataactgc acggattttg ggacaattat caaacagata tttgtcccga
    27421 ttcccgtttt accgaaaaaa gaaaggggga tcgattcctg tgacaaaaga agaaatttta
    27481 acgggacccg gtgtcataaa gctcgttagt caatacatgg gtcccgaaca tgtagcattt
    27541 gttgagaaag catgcgaata cgcgactgct gcacatgacg gacagtttag aaaatcaggc
    27601 gaaccctaca ttattcatcc tatccaagtc gcaggtatat tagcagattt aaaaatggat
    27661 ccccatacag tggctacagg cttcttacat gatgttgttg aagatacaga aatcacttta
    27721 gaagatctga gagaagaatt tggcgatgac gttgctatgt tagtggacgg cgtaaccaaa
    27781 ttagggaaaa taaaatataa atcccacgaa gagcagctag cagaaaacca ccgaaagatg
    27841 ctgctagcaa tggctcaaga tttacgagtt atcatggtca aattagctga tagactacat
    27901 aacatgcgta cgttgaagca cctgcgagaa gataagcaaa gaaggatcgc tcaggaaact
    27961 ttagaaatct atgcgcctct tgcacatcgt ctagggatca gccggatcaa atgggaatta
    28021 gaagatacag cacttcgtta tctaaatcca aaacagtatt accgtatcgt ccacttgatg
    28081 cagacgaaga gagaagaacg tgaaaaatac gtaagtggta ctgttgaaga tattcgaata
    28141 gcgacagaag agctggggat ttttgcagaa atctatggac ggccaaaaca catttattca
    28201 atctatcgta aaatgaaaga tcagaaaaag cagttcaacg aaatttatga cttgctagct
    28261 attcgagtga tcgtagattc aatcaaggat tgttatgctg tattaggagc aatccacaca
    28321 aaatggaaac caatgcccgg cagatttaag gattatattg ccatgccaaa agctaatatg
    28381 taccaatctt tgcatactac cgtgatcggt ccggcaggaa atccggtaga aattcaaatc
    28441 cgaacacagg aaatgcatga aatcgctgaa ttcggggttg ctgcacactg ggcctataaa
    28501 gaaggaaaaa atgaaaaagt agaaccagat ggtatgacga aacaattaag ctggttccat
    28561 gagatactcg aacttcaaga cgaaagctat gatgcttctg aatttatgga aggcgtaaaa
    28621 ggagatatct ttagtgataa agtctacgtc ttcacaccaa aaggagacgt tactgagtta
    28681 ccaaaaggat ccggaccatt ggactttgca tacagtatcc atacagatat cggtaacaaa
    28741 accactggtg caaaagtaaa tggcaaaatg gtgcagcttg attacaaatt gaaaaacgga
    28801 gatatcattg agatcatgac ttctccaaat tcatttggcc caagtcgcga ctggttgaaa
    28861 ttagttgcta ctagcaaagc aagaaataag atcaaacgtt tcttcaaagc ccaagatcga
    28921 gaagaaaatg tgatcaaagg ccacgaatcc gtggtcaaat gtattacaga tctaggattt
    28981 acgcctaaag atattttgac gaagaacaaa ctgcaagaag cactcgatcg ttttaattat
    29041 caaacagaag atgatctcta tgcagctgta gggtatggag aagttagccc cttgacgatg
    29101 gccaatcgtc tgactgaaaa agaacgtaaa gaacaaaaaa tcgagcagca aaagcaagaa
    29161 gcagaagaaa tcatgaatca gccgaaaaaa gaacctgaca aaatgaaagt acgtcatgaa
    29221 ggtggcgttg tcattcaagg ggtagaaaac ttactaattc gtatcagtcg ctgctgtaat
    29281 ccgattcctg gtgatgatat cgttggttat atcactaaag gcagagggat atccattcat
    29341 cgtcgagatt gtccgaatgt tcagcctgac aaaccaaatg tagcagaacg tttgattgaa
    29401 gtcgaatggg aagatacatc gaatacacga aaagagtatg atgcagattt ggaaatttac
    29461 ggctataatc gttcaggctt attgaatgat gtacttcaaa cagtcaatgc gctaacgaaa
    29521 aatctcaaca gcgttgaagc acggacgaat aaagataaaa tggcgacgat ccatttgacg
    29581 gttggtatcc agaatttatc ccacctaaag agtatcgtgg ataaaatcaa agcagtacct
    29641 gatgtctaca gtgtacgccg gacgaatgga taggaggaca gatatgagag cagttgttca
    29701 acgggtaagt aaagctgctg tgtcagtcga tcagcaagaa attggaaaaa ttggctacgg
    29761 cctacttgtt ttattaggca ttcatgaaac cgatacgcaa aaagatgtgg actatctggt
    29821 aaaaaaaatt gcacaaatgc gtatttttga agatgaacaa ggaaaaatga atattagtgt
    29881 agaagatgtg ggaggacaaa ttttaagtat ctctcaattc acactgtttg cggatacgaa
    29941 aaaaggaaat cgaccaagct ttgtggaagc cgcacgacca gaaacagcta ttcctttata
    30001 cgaatcattc aatgaaggaa tcagaagtcg cggcatcaca gtcgctaccg gacaattcgg
    30061 agcagatatg tcggtttctc tcgtcaatga tggacctgta acgatcatta ttgattctaa
    30121 aaacaaataa agtaatctgg ttttcactag taaaaactat gaaacacgtc gcttaagtta
    30181 gactgataaa ctctaactta agtggctttt ttgtctgcaa aaagcgcttc ttttgtcagc
    30241 agtcggaaaa aaagttaagc ttaaattata caagttattt tttataggag gttgctgaag
    30301 atgaaagaag taccagtagt agatatcaaa atcacagatt taaaaaaatt gtatcaaaaa
    30361 ggatacaaca tgttggagga gttacgccat gaagcggacg ctcccgtcgt caaagcaaag
    30421 attttcaata aagaagctat cacgatctac ggatcatcag cagcaaaagt attttatgat
    30481 ccaaggaatt tcaaacgaaa aggtgctatg ccaaaattag tattgaagac actttttggt
    30541 caaggtggag tacagacttt ggatggagct gctcatcacc atcggaaaaa tatttttatg
    30601 gatctaatga caccggaaag aatggaagat tatcatcgta tcttagataa gaatctgaca
    30661 caagcacttg aagcacagca tggtcaattt gaactatttg atttaagtaa gatggttttc
    30721 tttacatcta tttgcgagtg ggcaggtatc aatttatcag cgatttctaa agatgaggtc
    30781 gaaaaattag cagaatatca aatctctatg atcagcggaa cgtttacttc tccgattgat
    30841 cacataaaag gtgtcgaaaa tcgcaaaaaa tcagaaaaat gggcgcaagg cttgattgaa
    30901 gaagctcgac aaaatcctgt ggctggaaaa gaaaatgtgg cattatatgc ttttgccaat
    30961 gccacagatt tagatggtca gcttttacct ctggaagtgg cagctgttga attattgaac
    31021 atcatccgtc caacggttgc tctaactgtg tgggcggcat tgatgggaca tgcacttttc
    31081 agtagaccag atttgtatca gcaattaaaa aatgactttt cgacgttaca agatccgttt
    31141 attcaagaaa tgagacgcta ttatcctttc ttcccaatgc tacctgcgat ttcgctgaaa
    31201 gaagtagaag tagatggtta tcgtatacca gaaggcagtt gggttatttt agatttatat
    31261 ggaacagacc atgatgaacg aacagttgaa gcgccagatt cctttatgat caaacggtat
    31321 gtagggaaag caaaagatat ttcctataaa gaagaatatg agatgattgc acaaggcggg
    31381 ggaaattttc gacaaatgca tcgctgtgca ggagaatgga tcactcttca tagtctacgt
    31441 gtgttttccg atcagctagt aaataagttt gagttctctg tacctgaaca agattggacg
    31501 attccattca atcagtttcc gacttatccg aatagccgag cgctacttta caagaattag
    31561 attagcataa acttatcgaa aaaaaggaag atcaaacagt gctgatcttc cttttttcat
    31621 attactataa attgataagg tcaattgatc aaaatccacg aaatgcttca tcaatggttt
    31681 gataatcttc attggataaa tgcaaatcta aagcttggac attgtcagat acttgctctg
    31741 gacgcttagc tccaggtata actacagaca ctcgtggatt cttcatgtac caagctaaaa
    31801 caagttgcgc aggagtggct tggtatcttt tagcaatggg gcgaagtaca tcgacagcgg
    31861 ttaaaatttc tctgaaacgt tctccttgga aatctggatt tctttttcga ggatctcctt
    31921 caccaaattg tttttcctca ccaagttcat attttcctgt cagcaaaccg gaagcaagag
    31981 gaaaataggg aacaaaactg attttgtttt tttcaagata agggaataat tctttttcag
    32041 cctgccgatg gatcaaactg tatttatctt ctactacatc gacatatcca tctgcattcg
    32101 cttctttcaa ttgttctaac gtgaagtttg acacaccgac tgctcggatt tttcctgctt
    32161 cttttaattc gtgcaatgta gcaactgatt cattttttgg tgtgctttca tccggaaagt
    32221 ggatataaaa aatgtcgatg taatcagttt gcagacgttt tagtgcatct tccaccgatt
    32281 gtttgagaaa ctcaggagaa ttatcaaatg ttcgcccttt atttggtaca tgagcagctt
    32341 ttgtagcgat gacgacacgt gagcgatcat attctttttc cttcagtacc tcgccaatca
    32401 gttcttcgga acggccgttt ccgtaagcat aagcggtatc gatcaaattg atcccgctat
    32461 tcaaagctgt acgaactact tgttttcctg tctcatcttc caaaccgtca aaaagattat
    32521 gtcctccaac ggcatttgct cctaatccaa gctgttctgc gtagacttgt gaatgtccga
    32581 ttcttatttc tgccatagct gattcctcct ttttgtctag tctaaaacaa aataccaaaa
    32641 agctgaacta aaacgttttt gtgaggggaa atagtcagat atctcttgac tttgagactg
    32701 gtttttagta ggttataagt aatatcgaat gtcaatgaaa gagcgagtaa gtttttgaaa
    32761 ctttacaaga gaggatcatc gaggctgaaa atgatctaaa gtttcattag cctaaagaca
    32821 ctctggagat ggacgagtga aaagccgtcc cgcgtacgag cgttattcgt aagaggaaag
    32881 agaaatcttt caaaattagg tggtaccgtg tatgaatttc attcaccctt gttcatgatg
    32941 aacaagggtg atttttttat ttacatagct aagaaaggga gagaaaacta tgagtttcca
    33001 aagaccaaaa ggaacaaatg atattttgcc aggcgaatca gaaaaatggc agtttgtcga
    33061 agaaacagca cgtttattat tcagagatta tcagtataat gaaatgcgta caccgatttt
    33121 tgaacactat gaagtgatat cccgaagtgt aggggataca acggatatcg tttcaaaaga
    33181 gatgtatgat ttttatgaca aaggggaacg ccatgtgacg cttcgaccag aaggtacagc
    33241 accaatcgtt cgttcttttg ttgagcataa attgttcggc ccagagtatg cgaaaccgtt
    33301 caaaactttt tatatgggac caatgtttcg ctacgaacgc cctcaaaaag gacggttacg
    33361 tcaattccat caaatcggag tagaagcatt tggatcagaa aaccctgcaa ctgatgtgga
    33421 gagcatggtg atggcacttg atttcttcaa acagttgggg atcacgcaaa tccggctagt
    33481 catcaattca ttaggtgata aagaaacgcg tcaagcctat cgtcaagcat tgatcgacta
    33541 tcttttacct tttgaatcag agctgagtga agactctaaa cgccgccttc atgaaaatcc
    33601 attacgcgta ttggacagta aagacaaacg agatcaaaaa tttgtggcag aagctccatc
    33661 tatcttagat tatctaagcg agcctgctca aaaacatttt gataccgttg tttccatgct
    33721 ggatgcattg gctgttcctt atgaaatcga cagtaatatg gttcgcggac tagattatta
    33781 cacacatacg atttttgaaa tcatgagtga cgcaccaaaa atgggggcac aagcaacgat
    33841 ttgcgcaggc ggacgttatg acaatctagt ggaagaacta ggtggtcctt caacacctgg
    33901 cttcggattt gcaatgggta tcgagcgatt actcttgact atggaggcag aggaagtagt
    33961 tatccctgca tttaatgagt tggatgctta cgtggtcgca ctcggagacg aaacaaatat
    34021 cgaagcattg aaagtggtcc aagcgattcg aaactttggt ttttcagctg accgcgactt
    34081 catgaaccgt aaagcaaaag cgcaatttaa gacagcagat aaagcgaatg ccaaattagt
    34141 attggtgatc ggtgaagatg agcttgctag tcaaacgatt aatgtcaaag caatggcaag
    34201 ccgaaaagaa aaaagttttc cattagaaga gatatatgaa cattttgatg aagtctatga
    34261 tgaaatgact ttattcgagt aggaggaaaa aaataaatgg cacaaagaac gatctattgc
    34321 ggattagttt ccgaacaaca attagaacaa acagttacat taaaaggatg ggtacaaaaa
    34381 agacgagacc ttgggggagt aatctttatc gacctacgtg atcgtgaagg aatcgttcaa
    34441 atcgtcttta atcctgaaat caacaaagaa gcatgggaaa tcgcggataa atgtcgcagt
    34501 gaatacgtga tcgaagtgac tggggaagtt agaagacgcg gagaaaatgc agtaaatcct
    34561 aaaatggcca ctggtgaatt cgaagtgatt gcaagtgaaa tcgtgatttt gaatcaagca
    34621 aaaaccccgc catttcaaat tgaagatgat caagcaatca gtgacgaaat acgtttgaaa
    34681 tatcgttatt tggatctgcg tcgtccaaaa atgactaaaa acatcaaatt aagaaatgcg
    34741 acaacaaaag cgattcgtca ttatttagat gataatgact ttctcgatat cgaaacacct
    34801 tacctaggga aatctacacc agaaggcgca agagactacc tagttccttc acgtgtccat
    34861 gctggacatt tctatgcgtt gccgcaatct cctcaattgt ttaaacaatt gctgatgaat
    34921 gctggattcg accgttatta tcaaatcgtt cgttgtttcc gcgatgaaga tttacgtgga
    34981 gaccgtcagc ctgaatttac gcaagtagat attgaaacaa cgttcttaac agcagaagaa
    35041 atccaaactt acacagaggg attgattgcg aaggtaatga aagaagttcg cggtatcgaa
    35101 gtcacattgc catttcctcg tatgacctat gatgaagcaa tggctcgcta cggcagcgac
    35161 aaaccggata cacgttttga tatggaattg atcgatctaa gcgatacagt caaagaagta
    35221 gaattcaaag tcttccaaat ggctcttgaa aacggcggtg tcgtgaaagc attgaatgct
    35281 aaaggcgctg cagatcgcta ctcacgaaaa gacatggatc agttaggtca atatgtagga
    35341 caattcggag ctaaggggct tgcatggctg aaagtagaag aagacggatt gaaaggtcca
    35401 attgctaaat tcatgggaga agcaacagaa gcaatcatta aagcaacaga tgcaaaacca
    35461 ggtgacttgc tgatgtttgg cgcagataaa agtgaaatcg tcgctgctgc attaggtgct
    35521 atccggacac gtttaggaaa agaattagga ctaattgatg aatcgaaatt caatttctta
    35581 tgggtaactg attggccaca atttgaattt tcagaagaag aaggtcgata tgtctctgct
    35641 caccatccat tcacgatgcc aaaagaagaa gatatcccgt tattggctga agaccctgca
    35701 aaagtctatg cagaagctta tgatatcgtc ttgaacggtt atgaactagg cggaggttct
    35761 atccgtattc atactcgtga gcttcaagaa aaagtactag aaactctagg ctttacaaaa
    35821 gaacaaatgg aagaacaatt tggtttcttg ttaacagctt tagattacgg attccctcca
    35881 catggcggta ttgcattagg attggaccgt tttgcaatgt tattagccgg agaagataat
    35941 attcgtgaag tcattgcctt tcctaaaaac ggaaaagcag cagatgtgat gagtgatgca
    36001 ccaagtatgg tttcgccttt acaattgttc gaattgaata ttgatgtaac gaatatagaa
    36061 gaatagccga taaaaaggta aaaaggattt gtcgaatacg cttcttttga caaccttgat
    36121 ttaattgaaa aacactttat aatgaagcca taacatataa agcggggctt ttccatgaaa
    36181 aaaacattcg aaaattgggc ttatcatgat tacacgacca agctttcggt atctatcgtt
    36241 tatgcgttga tcgcatcagt cgcattgaat ttcttttatc agccaggaca tatttattca
    36301 agtggtatta ctggtctagc tcagattttg acgactttgt caaaatcact gataggattc
    36361 gaagtaccgg tatcaacaac tttgtattta ctgaatatac ctttgtttgt attggcgtgg
    36421 ttaaaaatcg ggaagaagtt tacaattttc acttttatca cagtcacttt gacctccctt
    36481 tgtatccata ttttaccgga aacagtccta tctaccgatc caatcatctg tgcgttgttt
    36541 gggggggctg tgatgggact aggtattggg ctagcgttga gaaacggcct atcatcgggc
    36601 ggtctagata tcgtgagtat cacgattcgt aaaaaaacag gaaagtcgat tggttctatt
    36661 tcgatctact tcaatgcatt gatcgttttt gttgccggaa ttctatttgg ctggcaatat
    36721 atgttttaca gtacgttgtc tattttcgtc agtggaaaag taactgatgt cgtttatact
    36781 aaacaaaaga agatgcaggt aatgatcgta acgaaacatc cagaaaaagt agtaaatgaa
    36841 atccaaaact gtatgcgtag agggatcacg atcatcaatg aagcagaagg tgcttataag
    36901 catgataagc agacagtatt gatgacgatc gttacacgat acgaactgcc tctacttaga
    36961 gaagcaatga aagcttctga tccacaagct tttgtaagta ttgcagaaaa cgtccagata
    37021 cttggtcgtt tctatgagga tggcatttag atcgagtttg acaaaaattc atgaaatact
    37081 gctgtagaca gacgttcgtt tgtctgcagt ttttttctac catagtagaa tcagcaaaga
    37141 aaaactgctt ttttatttga aattttctta aagttgaaaa aaagcatgtt tattttttta
    37201 aatttatggg atgatagaaa aaaatagtag agaaaggaca cgattctact tgctattttc
    37261 acttaatacc cgtatattac gaatgaaaaa agtttatgaa aagttggaat attgaatgga
    37321 aaaacaaaaa aggttaaaaa acagccgcta catcactggg tttgatgggc tccgttcatt
    37381 agctgtggtg ggtgttatct tctatcacct tctgccaact agtatgaaag gcggctattt
    37441 gggcgttccg atttttttcg tcgtatctgg ttatttgatc acggatctgc tgcggcagga
    37501 atgggaacaa aatggaaaaa tcaatatttg gcaattttat gtcagaagaa tgaagagact
    37561 ctatccggca cttgttttta tgcttgtgac agcttcggct tatatcactt tgtttcagcg
    37621 agggctgtta aacaatcttc gaggagttgt ggtcagcagc ttactgtatg tcaacaactg
    37681 gtggcaaatc aataacggtt tatcttattt cgatcgattt gctaatgagt caccgtttac
    37741 gcatatctgg tctttagcag tagaaggtca aaactatttg gtttggccga tcttgtttgt
    37801 attgttgatg gtatttgttc gtaaaagaaa atggatcttt tatactgtgt taggtacatc
    37861 cttcctctcc gccatcttga tgatgatctt gtatacacca ggaggcgatc caacccgtgt
    37921 ttattacggt accgacactc gtctgttttc tatatggttg ggaagtgcgc ttgcgtttgt
    37981 ttggccaagt acgcgtttaa agaaaaacat cccgaaacaa gcgaaaaata ttctgaatat
    38041 cgctggagga atctcgcttt tggcactgat tctttgcttc ttctttttag atgatcatta
    38101 caatttcatc tattacggag gaatgctcct agtgagtctg ttatgcgtag tgctagttgc
    38161 agtgacagca caccccggag caagtttgaa tcgatggctg acaaatccag tatttacttg
    38221 gattggcaaa cgaagctatg gcatttatct gtatcagttt ccagttatga ttttttatga
    38281 agcaaaggtg actgatattg ccgatcatgt tttgttgcat actttagtgg aaatcacatt
    38341 gattctaatc atcagtgaac tttcttatcg atatgtggag cgtccgctag cacgttttga
    38401 ttatggtcgt acattccaag tgatgaaagg atggttcagc aaaccgatca tcagtaagag
    38461 caaaccatgg gttgttccag gcacattact tgtagcaatt gcactcgttg gtttcgttac
    38521 ggcaccgaaa aacgctgtaa cagccgatca aaaacaactg caggcaaaaa tcgctgaaag
    38581 taaaaaatta gcagaagaaa ccagaaacaa ttcagaaaac accactagtt cggttgacca
    38641 agcgatcctt gataagtacg agttgtccaa ggcacaaggg aataaggcac aaaaattaga
    38701 actaacggca tttggtgatt cagttatgtt ggatgctgca acggatctac aagaacttta
    38761 tccaaaagta gtggtagatg gagatgtcgg acgacaatta tatgccagtg taccgtacat
    38821 tgaaaaatta aaggaacaag atttgttgaa agacactgtt ctgatcggac taggaacgaa
    38881 tggctcattc tcggaagctc agtttgatag tgtgatggat gcaatcggtg atcgaaaagt
    38941 atactgggtc aatgtccgag taccgacgaa gcgctggcaa aatgacgtga atgcgatgct
    39001 tgaacagatg acgaaaaaat acgacaatat gacgctgatt gattggtaca gctacagtaa
    39061 tgaccatagt gactggtttt atgatgatca agtccatcca aatcctgaag ggatgacaca
    39121 ttacattcac ttagtcagcg aagccttgct aggaggaagt tcaagtacga acagcagtga
    39181 gacaaacgtg acagaatgat agctacaaaa aacttcaggc aaaaacattt gtttttgcct
    39241 gaagtttttt tatatttacc gtgtttttaa cattcatata gattcaggaa gcaaaacggt
    39301 tgagtgtgtc tgtaaataac gttctttttg ttttcgggtg agttttttga atgcagcttc
    39361 tgcttttgtt gcttcgcttc ttgaagaaaa tacttctgca tggatcatct taacaggtct
    39421 tcttttttga agacgagtat atttagcacc agtaccattg ttatgttcct ctaatcgtct
    39481 agaaagatcg gttgtatatc caccataaaa tgtctgatct ttacagaata atacgtaaaa
    39541 ataatgttca tttgccatag agcatctcac gaatctctgc agtgtattct ccatccatat
    39601 tgtaagtgat caatggtgga gcgactttga atccgtctaa tttgccttct ttgattcctt
    39661 caactaacaa aatatttgcc tcttttccag gttttgggta aacaaattgg atccttttag
    39721 gtgcaattcg atagtcttcc atgacctgaa tgatttctaa gaaccgatca ggacgatgga
    39781 ccatagtaaa gcgtccattt gtttttagta gcttacttga aatatcgatc acttcttcta
    39841 acgtcgtatg aatctcatga cgggcgattg ccaggtgttt attagggttc gttttatttg
    39901 ttggcagccc tttgaaataa ggtggattac agacgactaa gtcacaagaa ttatgtcgaa
    39961 cgacagataa gctgtctttc agatcgatgg tgtggacagt catttgttca tttaaatggt
    40021 taagcgcgat actacgtctt gccatgtctg ctagacgagg ttgaagttcg atagcgtcga
    40081 tagccgcctg ggttcgttta cttaaaaaaa gtccaactgc gccatttcct gcacaaaggt
    40141 caacaatctt ccctttttta ggtacacggg aaaagtgagc aagcagcaca gcatctaaag
    40201 aaaaagaaaa cactttggaa ctttggataa tcttgatatc ttctgcgtat aattggtcga
    40261 ttcgttcgtc ttcgtgaagc atggattccc tccaaaatga aaaaaatagt cgtctgtatt
    40321 atactatttt tattaaaaag aaccaaggtg gcttgtcaaa cctctatttt ttatgcatac
    40381 tatgaccata agaagggaaa ggaagagact atgttttatc gatttatgcg cggggtagtt
    40441 cgagtactat tagccttagt taatggaaat gcccgctatg agaataaaga agttttaccg
    40501 caagatgaga attacatact agttgctccc catcgtactt ggtgggaccc attatatctg
    40561 gcggtagctg cagcacccaa ggaattcagc tttatggcaa aagaagaact gttcaaaaat
    40621 ccagttctgc gatttatatt aactcgatcc aatgcttttc cagttaaaag agaaaatcca
    40681 ggaccaagcg tcattaaaac acctgtaaag attttgaaat cgacaaattt gagtttgatc
    40741 atgtttccca gtgggacaag acatgctaca gaacttaaag gcggaatggc attgatttcc
    40801 agaatgtcta aagcgaaaat cgtgccagct gtatatcaag gtccgttgac acttggggat
    40861 ctgttcaagc gtcagagagt gacagtgcgt ttcggcgaac caatcgactt atcagatatc
    40921 aagaaaacgg ataaagaagg tatggcggag atcgaacgac gaacacaagc agcttttgac
    40981 aaattggaca aagaagtgaa ccctgatttc aaatatgaag tcaaaaaaaa ataaaatgat
    41041 aagtttatgc gtgggcggtc ttttgaatct actgtcctgt tcagttcaga ctttttcaat
    41101 gaaaacgagg gagagacgtc aagtctgccc tctttttgcg ttccaatatg taaaatagaa
    41161 gacaggtgtc atgtaatgga tcaaagtgtt ataataatta gaaggacatt ttttgaggtg
    41221 attgaatgcg ttttttacat acagccgatt ggcatatcgg aaaaaaacta caaggttatg
    41281 acttgattga agaccagcgt catgtactat cttctatact agatatcgcg aaagaagaaa
    41341 aagtagaggc aatcgtgatt gccggagact tatatgatag gagtgtccca gctgttgatg
    41401 ccgtagaatt atacaatgaa ttgatggtcg attggaattt aaaggaaaag atcccgattt
    41461 ttgctatatc aggaaatcac gatagcagta cgagactttc agcgggtacg ccatggttca
    41521 gttattcgaa ctattttttg cgaacacagc tagcagaagc atttgaacca gtcgagttta
    41581 aaaacgtaca atttttcttt cttccttact ttgaaccaat tgcagcgaga ttatattttg
    41641 aagatgacaa tatccgaacg atcaagcaag cggtagccaa ggtgatagat aaaatgcagg
    41701 agctatttga ttcagaaaaa aaacaagtat tgattagcca tttttttgta atgggaagtg
    41761 taaaatctga ttcggaaacg aaaatcgaag taggcgggtt ggattcgatc cctggtgaac
    41821 tacttacacc gtttgattat gtagcactag gacatttgca taatcaagct gctttgagac
    41881 aggataatgc tcgctatagc ggttccccat tgaaattctc tttatctgag atcaaccaaa
    41941 aaaaaggcgt atggatcgtt gacctgacaa caggcctgga gttgaatttt agggagatcg
    42001 aacccctgca tgatattgaa gaaataacag cgagtttcaa agaattgctg gatcctgcct
    42061 tttatcagtc cgtcaatcgg gagaattaca tctatatcta tctcactgat agaacgatca
    42121 tcccgaatat gatgaaccag cttcggcaaa tatatcctcg tattttgggg attgaacggg
    42181 caaacggtcg agcagaacaa acaaagcgca aacgaaaagt actagaaatg aaagatcctc
    42241 aaaaattagc agaagcattt tttagtgaaa tgacggaaga gcagctaact gcaaaccaag
    42301 aaaaatggct cggagaaacg ttgcaagaaa tcatggaaat ggaatgatca agaatgaaac
    42361 cgaaaaaatt acaacttaaa aattttggtc cttttatcaa tgagaccgta gatttttcac
    42421 gtttgacaga agcaccgctt tttttgatca gcgggaagac aggagctggc aaaacgacga
    42481 tttttgatgg gatcaccttc gcactttttg gtgaaacttc cggaagactt cgctctggaa
    42541 aagagatgcg ttctttgttt gctacaccag acgaagaaac aagcgtgact ttttcttttg
    42601 aacatcagca gatgagctat gaaatcgaac gaaaaccaga acaagtttta gcaaaacgaa
    42661 aaggaaacgg aacaaaaaaa caggcagcca aagtaacgtt gaccattttt gatgggaaag
    42721 gaaaagaaat aaaacaaatc acaaaacgta cagaagcaga tcagttgatc agagaactga
    42781 tgaatctcga tgcgaaacag ttttctcaaa tcgttttact accccaagga gaattccgta
    42841 attttttggt ttcatcaagc agtgataaag agactgtttt acgtcattta ttcggtactc
    42901 aatttttcca acagttcaat gagcgattaa aagaaaaagc aaaacaacag caacaaaaat
    42961 tggatcattt agagcaagag ctgcatcttc tgcaaaaaag gtttgtttta gtcaaagaag
    43021 aggaaccagc agcagggttt gaagccgttt tagcccaatg gacaaaccat caggcaatac
    43081 tcgaaaaaca aatcacggaa gaaaaagaag ctctaacgga acttcaggaa aaacaaaagc
    43141 aactagaaaa ttcctactat cgttttgaaa gcttgaaaaa aagttatgaa gaaaaaacga
    43201 tattaataaa caagcaaaag gaattagctg ctcaaaaaga cgaaatagag gaaaagaaac
    43261 gatggattgg gtattatgaa tttacgcaac gattagtcga accaatccag cgcctacaag
    43321 aaataaaagt tgaaaaaata gaactgtcac aaaaaagtag tgagcaaaca aagaaactag
    43381 cgatgactac gcagaattat gaagagtggc aaaaaaatga agcaaaccgc gtacgccgac
    43441 aaacaaaaat caaagaagct gctcaaaaac tgcaaaacaa acagatgatg gtgccaattg
    43501 tcgaatcctt acgtagtaaa aaacaagacg cagaacaatt aacaatttca ttggataaag
    43561 aagaattgaa tgccgagaag ctgtatcaaa atctcgctat ctatcaggaa agaactcgaa
    43621 ttcttcaaga aaatttactg gatcaggaaa aactgcaaga agatcgtctt gcttttggac
    43681 agttaaaata tctagatgaa aagcagcaag aaatagaaaa acaaaaagca aaagacactg
    43741 ggtcgctcgc acagataaga gaagaactca aaaaagcaac tgaagaaata acaaaaaata
    43801 gtcagctatt gaaagaacag acagaacaag cagaacagct gaaaagccaa tgggcaaagc
    43861 agcaaattgc tcgtcttcag ctttttttgc gtccagggga gccatgtctt gtttgtggat
    43921 caacggaaca ccccatacaa caattaaaac acgaacagcc aaataaggaa gatatcttaa
    43981 aagtagaaca acaactggca gaagctgagt ctgctgtaga aagaactcag caaatcattg
    44041 ccaaaagtga agcacatgaa aataatcttc aacaacaatt atcagattta gaacaaaaaa
    44101 tcatggcaac gaatatacaa ttggctgaac aagaagaaca cttgcaacag gctttgaaaa
    44161 aggcatttcc tgagataaca gaacaaaaaa caaaaacaac tcttgtagta atcgaacaat
    44221 ctttagatag acgaaaaagc aagatggcca atgcaagaat agagctggaa gaaataagac
    44281 aacaagcaga aaagctcgaa caacaattag ctgtctacaa acagcagcag acagaaaaaa
    44341 ataatcagct ccagcaactg caaggagaaa tcaaaagttt agaacaacaa ttgcaagata
    44401 cggatagtaa ctgtttagaa gaggagatac gacgacttga aaaacagtta gaagaagact
    44461 cgaatcaact agctgttgag cagaaagcag gagtgcaatt gttgcaagat cttcatacgc
    44521 ttcggacaca gcttgatctt caacatgaac aaatagaaaa aatagatgaa aaaatagaaa
    44581 aggaacaaga aagaatagct aaaaagttag aaagccagtc gtatttttca acaattgaag
    44641 aaatctgtgc atttgcacaa cagcaagcac aatataaaga aaaccaacgt acagttactt
    44701 cttatcatga agagcaatta gtgacagctg accggctgcg tcaattagaa aatatccagc
    44761 taactgagac attcccagat acagaagaga tcagccaaca gttagaagaa ataagacgta
    44821 tgatcgaaat aaagcaagaa aagatatacg gtttacaaga acaaaaaatt agtaatcaaa
    44881 aaatctacga tgaatgtctt tcgatttatc acacaagcca gacgaaatta gatgagctta
    44941 cgcaattaca gcagctttcg caaacattca acggagaaaa tcctaagaag actagtttgg
    45001 aacggtatgt gcttcaggtt tatcttcaag aagtgttaca agtagcaaat gatcatctcc
    45061 aacgtttaac gaaaaatcgt tatcaatttg agctagcgga taccattggc agttatcgag
    45121 ggaaaaccgg attagaaatc aatatctatg atgatcaggc aggtatgtct cgaagtgcgc
    45181 ataccctgtc tggcggtgag agttttattg cagcactttc tttagcatta gctcttgccg
    45241 atgtgatcca gacacaagct ggtggtgtag caatcgatgc attgtttatt gatgaaggat
    45301 ttgggtcatt agatgaagaa gctttggaaa tggcaatgga agctttagaa acgatcgaaa
    45361 gtgaaggaag aatgatcggc atcattagtc atgtaagaga attgaaagaa cgagtattac
    45421 aacaaataag aattgataca gaaggtagcg gacaaagcaa aatacgctat caacttggat
    45481 aaaaagggag attggtaact atgaaatgga gtattccaga aaaagtcatt gcacgtggac
    45541 gtcaatactt gcaagaaggc cgagtgttat cggttgtccc tgaccaagaa gaaaacgtgt
    45601 ggcatgcaga agtcttgggc agtgaattgt atatggttga tttggatggt acagcaaaag
    45661 agatagatta ctgccaatgt ccctattggg aggaacataa atattgtaag catacagttg
    45721 ctgtggaact ttatttacgg tcgaaaaatc tatctcgtat catgaaaaaa gaccagccaa
    45781 tgctagaacc ggtaaaagca acaagtgagg gagaaatcat tgcaaaaggt ttttctcgtc
    45841 taaaaacaca aaacaatcct caagcggtgc aacctttgat tattgaatac caagtagaaa
    45901 cgattgagac taatcagtat cacccagagc tttctatttt ggcactttac ttgcgtatcg
    45961 gttcattggc atcaccgaaa aaaacatata tcgtaaaaaa catctatgat tttttacaag
    46021 cttacacaga aaaagaaact tacactgtta ataaacagta ccagtttcgt ctagataaac
    46081 gggcgtttca aaaagaagat caaaaaatac tcacgcatct tgcaggaagt gctcagacgc
    46141 atcaattatt aggtacgaac ggcttacaag tcaaaggaaa actagataaa agatatctgc
    46201 ttctcccagt agaacaagcc cgcttcctgt tagagcagat gaatcagact acacgtttgc
    46261 atctgcagat aaatgaacaa aacgatcaag gaatacaatt ttccgatcaa aacaaacctt
    46321 tatcattcaa agtggaaaaa tttggagaag attatttgct gcaagcattg aatgattttg
    46381 attttttctt tatgcattat cagtggggag ttatccaagg gacattttat tctctgacga
    46441 aatatcaaca aacggtttat caaacttgga aacaattgat tcgccgattg gagaaaccag
    46501 aagtagtgtt caagaaaaaa gaattaccgt ctttgtttaa agagattatc cctctgctgt
    46561 cagagatcgg cgacgtagct attgcccctg cagtagaaga ggaagtagca gatatccccg
    46621 tcgaatttat tttctttttc cgaaaagcaa aaggaaaaat ccaagctcga gtcgatttcg
    46681 tctatggcga agtcatctat tcgactgacc cgaagcatga ggtctcgaca gaacaggctt
    46741 ttcaggtgtt acgtgacaca gtagaagaga aacgagtact tgatctattc agaatgtatc
    46801 gctaccagaa aaatgaaact ggttatgaac gtccattgcc aagcggggaa gaactatatg
    46861 cctttttccg cacagaacta tctgtcttcc gtcagtttgg tgaagtcaga cttggaagaa
    46921 aattgcgaga gttgtattta gatgcgcata aacatcaacc gaaaatcgaa gtaaccgaga
    46981 ccggttcatg gctcgatatt cgattcgatg tgacagggat agaggagcaa gaaatcgatc
    47041 atgtcctgca aagcttgctg agaaatgatg ctttttatac attagaaaat ggtcaggttc
    47101 tttcttttga ttcagaagaa tttcaacaaa caagccaagt gttgcaacaa ttacgggaat
    47161 cgatacgtac agaagaagga acgatccatg tacctaaaaa tcaaggattg atcattcaaa
    47221 atcaattgga aaaaagcaat gctactttta gtgaatcgtt ccaaacgatg gtacaagatc
    47281 tcattcatcc agaaagatac caggcacagc ttcccaaagg attgaatgca acgatgagag
    47341 attatcaaaa gcaaggtttt cgatggttga agatgttagg tcattatcag tttggcggta
    47401 ttttagccga tgaaatggga cttgggaaaa cactacagac aattgccttt ttactttcgg
    47461 aaaaagaaga acggaaaagc ttctctgctt tgatcgtagc gcctgcgagc ttaatttaca
    47521 actggcaggc agaggtgaga aaattcgccc cttctttaag tatccaagta atcaatggga
    47581 ataaaaaaga acgtgaagaa ttactggcta aagacacaga tatccgagtg acttcttatg
    47641 ccagcttaag acaggatcta gctaattatc agtcgcaaaa aattgattat ttgattcttg
    47701 atgaggcaca aatggtcaaa aatagcagta cgaaaactgc acaagcattg agagaattag
    47761 ctgtacctca gcggtttgca ctgagtggga caccaatcga gaataattta gaagaattgt
    47821 ggtcattgtt tgcaacgatt atgccaggat ttttcccgac taaaacaaaa ttcagggaac
    47881 ttgccgcaga agaaattgcg caaatgattc gaccgtttat tttacggcga gataaacaaa
    47941 ctgtgttgaa agacttgccg gaaaaaacag agatgaatct atattctgca cttacagaag
    48001 aacaaaaaac tgtttattta gcttacttgc gtcaaatgcg tgaagaaatc acatcaatgg
    48061 attctgaagc attcaagaaa aaccggatcg ggatacttgc tggtctgaca agactgcgac
    48121 aaatttgctg cgatcctcgt ctgtttattg aagattacca aggcggttcc ggcaagctcg
    48181 aacaagtaaa agatctactg gtagctgcca aagagaacaa acgaagagtt ttgctgtttt
    48241 ctcaatttac agggatgctg actatcttgc aagaagagtt agccgagcta gggatctcga
    48301 cattttattt aagaggcagt acgaaaccac aagatcggtt aagtatggta gacgcattta
    48361 atgctggcga aaaagatgtt tttctgattt ccttaaaggc tgggggaact ggattgaatc
    48421 tcacgggagc agatacggtc atcttgtatg acctttggtg gaatcctgcc attgaagaac
    48481 aagctgctgg acgtgcacat cggatcggac aaaaaaatgt ggtagaagtg tggcgtatga
    48541 tctctgaagg aacgattgag gaacgcatgg attcactcca gcaagaaaaa cgcgaactgt
    48601 tccaaaaagt gatccaaggt aatgaagaac agttgacgaa actgactgaa gaagacatcc
    48661 gcatgatttt aagtgtagga gaaatcgagg agtaagagac tcgaaaaagc gttcagtaat
    48721 gggtaagaaa gcctttctaa agaagcgatt gtttatttga tttaaaaaat agcaaggagg
    48781 ctgggattcc attttatccc agcttccttg ctatcatttt tttatttcta tttttaaagg
    48841 ttatacaaag attgtcttca ttgtctctaa cagctgatca aaagaagaaa tctcgatatc
    48901 gcaaattgga gtctgttcaa ttttgcgttc tcgatggttg aaccaaagcg cttgccagtt
    48961 agcacttttt gcacctagaa catcattgtc gtaattatcg ccaacgtata aggtctcttc
    49021 tggaagcata tgaaattctt tctcagctag ctgaaaaatc tctttttctg gtttttggaa
    49081 acctgttgct tgagaaatga gcatattgcg ggaaggaatc caattgttca attgaagttg
    49141 cttcagtttt tttgtctgat ggtcagtcgg cccatttgtg ataattccta atgggacatt
    49201 cttttccttc aagaaatcga gggttttttt gacctcctca tgcaagcaga tattatcaag
    49261 ttcttcctcg taaatttttt gaaataagag gccatcttct tccgtaatat gagggtagtc
    49321 taaatctttt agtgattggc tgatccgatg tgctcgcata tattctagtg tccaatctcc
    49381 tgccattact tttgggaagt tttcatcact atgatggcgg aaccggatat atagtggatg
    49441 catatctgca tctgaaacaa gcggaacgac tcttttgacc gcgtttcgaa atggctgctg
    49501 ctgatcatac atcgtatcat caacatcaaa cacaactgat ttaatcaaaa ttttacacgc
    49561 ctttcggttt tcattgattt tttcacaaat ccttgactat tgtaacagaa aaaattgtct
    49621 aaaaaagctg ataaataaag gtttttctta ctatatttct atttccttga cagaataagg
    49681 tttttatatt tcagtttttt tcactaaaaa tcgtgaaaca cgtcaataag tttgatgaac
    49741 aaattaatca ttatggtaga agtcgattga tttattttaa tgcaatctct gtggaatttt
    49801 cgaaagattt atttttttaa tacagtctca atataactat tcaaacataa aataacgtta
    49861 tttgatttat ttgtttatag taaattttat ttagtaatca ttgctttaaa atactacgct
    49921 tatataa
//
LOCUS       DACEOE010000005        49711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEOE010000005 DACEOE010000000
VERSION     DACEOE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.005"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW774_000234"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW774_000234"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1152614.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IW774_000235"
     CDS             complement(937..1518)
                     /locus_tag="IW774_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1152615.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW774_000236"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW774_000236"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1152616.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IW774_000237"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IW774_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1152617.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IW774_000238"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IW774_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1152618.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IW774_000239"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IW774_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1152619.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IW774_000240"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IW774_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1152620.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW774_000241"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW774_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1152621.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IW774_000242"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IW774_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1152622.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IW774_000243"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IW774_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1152623.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IW774_000244"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IW774_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1152624.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IW774_000245"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IW774_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1152625.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IW774_000246"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IW774_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1152626.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IW774_000247"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IW774_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1152627.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IW774_000248"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IW774_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1152628.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IW774_000249"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IW774_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1152629.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IW774_000250"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IW774_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1152630.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW774_000251"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW774_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1152631.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IW774_000252"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IW774_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1152632.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IW774_000253"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IW774_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1152633.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IW774_000254"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IW774_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1152634.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IW774_000255"
     CDS             13972..14157
                     /locus_tag="IW774_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1152635.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IW774_000256"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IW774_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1152636.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IW774_000257"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IW774_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1152637.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IW774_000258"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IW774_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1152638.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IW774_000259"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IW774_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1152639.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IW774_000260"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IW774_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1152640.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IW774_000261"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IW774_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1152641.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IW774_000262"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IW774_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1152642.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IW774_000263"
     CDS             18232..18879
                     /locus_tag="IW774_000263"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1152643.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IW774_000264"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IW774_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1152644.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW774_000265"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW774_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1152645.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IW774_000266"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IW774_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1152646.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IW774_000267"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IW774_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1152647.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IW774_000268"
     CDS             20317..21255
                     /locus_tag="IW774_000268"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1152648.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IW774_000269"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IW774_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1152649.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IW774_000270"
     CDS             21935..22774
                     /locus_tag="IW774_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1152650.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IW774_000271"
     CDS             22750..23619
                     /locus_tag="IW774_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1152651.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IW774_000272"
     CDS             23609..24406
                     /locus_tag="IW774_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1152652.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IW774_000273"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IW774_000273"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1152653.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="IW774_000274"
     CDS             complement(25688..28357)
                     /locus_tag="IW774_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1152654.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /locus_tag="IW774_000275"
     CDS             complement(28595..28783)
                     /locus_tag="IW774_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1152655.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="IW774_000276"
     CDS             29053..29415
                     /locus_tag="IW774_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1152656.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="IW774_000277"
     CDS             29467..31149
                     /locus_tag="IW774_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1152657.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="IW774_000278"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="IW774_000278"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1152658.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="IW774_000279"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="IW774_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1152659.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="IW774_000280"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="IW774_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1152660.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="IW774_000281"
     CDS             34984..35988
                     /locus_tag="IW774_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1152661.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="IW774_000282"
     CDS             35989..36933
                     /locus_tag="IW774_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1152662.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /locus_tag="IW774_000283"
     CDS             36933..37895
                     /locus_tag="IW774_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1152663.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="IW774_000284"
     CDS             37922..38836
                     /locus_tag="IW774_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1152664.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="IW774_000285"
     CDS             38862..40643
                     /locus_tag="IW774_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1152665.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /locus_tag="IW774_000286"
     CDS             40991..41677
                     /locus_tag="IW774_000286"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1152666.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="IW774_000287"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="IW774_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1152667.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="IW774_000288"
     CDS             45287..46096
                     /locus_tag="IW774_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1152668.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="IW774_000289"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="IW774_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1152669.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="IW774_000290"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="IW774_000290"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1152670.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
BASE COUNT        16453 a         9062 c        10740 g        13456 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tccgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacg atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga g
//
LOCUS       DACEOE010000006        47530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEOE010000006 DACEOE010000000
VERSION     DACEOE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.006"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            508..1698
                     /locus_tag="IW774_000291"
     CDS             508..1698
                     /locus_tag="IW774_000291"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1152671.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            1800..2753
                     /locus_tag="IW774_000292"
     CDS             1800..2753
                     /locus_tag="IW774_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1152672.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            2822..4171
                     /locus_tag="IW774_000293"
     CDS             2822..4171
                     /locus_tag="IW774_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1152673.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(4354..4677)
                     /locus_tag="IW774_000294"
     CDS             complement(4354..4677)
                     /locus_tag="IW774_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152674.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            4806..5969
                     /locus_tag="IW774_000295"
     CDS             4806..5969
                     /locus_tag="IW774_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152675.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            6039..7388
                     /locus_tag="IW774_000296"
     CDS             6039..7388
                     /locus_tag="IW774_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1152676.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7433..7864)
                     /locus_tag="IW774_000297"
     CDS             complement(7433..7864)
                     /locus_tag="IW774_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1152677.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            7931..8146
                     /locus_tag="IW774_000298"
     CDS             7931..8146
                     /locus_tag="IW774_000298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152678.1"
                     /translation="MEYDQKEMKIKIEILTVINRMRILNKYEKDMICFLRLTENSFLA
                     EKRIVTNQIAPFFYFGRNTDAAQRYRV"
     gene            8252..10894
                     /gene="alaS"
                     /locus_tag="IW774_000299"
     CDS             8252..10894
                     /gene="alaS"
                     /locus_tag="IW774_000299"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1152679.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            11094..11690
                     /locus_tag="IW774_000300"
     CDS             11094..11690
                     /locus_tag="IW774_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1152680.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            11812..12960
                     /locus_tag="IW774_000301"
     CDS             11812..12960
                     /locus_tag="IW774_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1152681.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(12998..13411)
                     /locus_tag="IW774_000302"
     CDS             complement(12998..13411)
                     /locus_tag="IW774_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1152682.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            13490..13921
                     /locus_tag="IW774_000303"
     CDS             13490..13921
                     /locus_tag="IW774_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1152683.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(14020..14253)
                     /locus_tag="IW774_000304"
     CDS             complement(14020..14253)
                     /locus_tag="IW774_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1152684.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            14436..16106
                     /locus_tag="IW774_000305"
     CDS             14436..16106
                     /locus_tag="IW774_000305"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1152685.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16253..16639
                     /locus_tag="IW774_000306"
     CDS             16253..16639
                     /locus_tag="IW774_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152686.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            16626..17324
                     /locus_tag="IW774_000307"
     CDS             16626..17324
                     /locus_tag="IW774_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1152687.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            17329..17793
                     /locus_tag="IW774_000308"
     CDS             17329..17793
                     /locus_tag="IW774_000308"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1152688.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            17790..18701
                     /locus_tag="IW774_000309"
     CDS             17790..18701
                     /locus_tag="IW774_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1152689.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            18936..19475
                     /gene="pyrR"
                     /locus_tag="IW774_000310"
     CDS             18936..19475
                     /gene="pyrR"
                     /locus_tag="IW774_000310"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1152690.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            19489..20772
                     /locus_tag="IW774_000311"
     CDS             19489..20772
                     /locus_tag="IW774_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1152691.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            20785..21711
                     /locus_tag="IW774_000312"
     CDS             20785..21711
                     /locus_tag="IW774_000312"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1152692.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            21730..23010
                     /locus_tag="IW774_000313"
     CDS             21730..23010
                     /locus_tag="IW774_000313"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1152693.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            23015..24091
                     /gene="carA"
                     /locus_tag="IW774_000314"
     CDS             23015..24091
                     /gene="carA"
                     /locus_tag="IW774_000314"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1152694.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            24096..27281
                     /gene="carB"
                     /locus_tag="IW774_000315"
     CDS             24096..27281
                     /gene="carB"
                     /locus_tag="IW774_000315"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1152695.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            27335..28111
                     /locus_tag="IW774_000316"
     CDS             27335..28111
                     /locus_tag="IW774_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1152696.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            28108..29040
                     /locus_tag="IW774_000317"
     CDS             28108..29040
                     /locus_tag="IW774_000317"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1152697.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            29037..29753
                     /gene="pyrF"
                     /locus_tag="IW774_000318"
     CDS             29037..29753
                     /gene="pyrF"
                     /locus_tag="IW774_000318"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1152698.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            29760..30389
                     /locus_tag="IW774_000319"
     CDS             29760..30389
                     /locus_tag="IW774_000319"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1152699.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            30377..30691
                     /locus_tag="IW774_000320"
     CDS             30377..30691
                     /locus_tag="IW774_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152700.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            30729..31370
                     /locus_tag="IW774_000321"
     CDS             30729..31370
                     /locus_tag="IW774_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1152701.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            31401..32105
                     /locus_tag="IW774_000322"
     CDS             31401..32105
                     /locus_tag="IW774_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1152702.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            32102..33004
                     /locus_tag="IW774_000323"
     CDS             32102..33004
                     /locus_tag="IW774_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1152703.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(33162..34868)
                     /locus_tag="IW774_000324"
     CDS             complement(33162..34868)
                     /locus_tag="IW774_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1152704.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            35058..35681
                     /locus_tag="IW774_000325"
     CDS             35058..35681
                     /locus_tag="IW774_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152705.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            36131..37180
                     /locus_tag="IW774_000326"
     CDS             36131..37180
                     /locus_tag="IW774_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1152706.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            37177..38058
                     /locus_tag="IW774_000327"
     CDS             37177..38058
                     /locus_tag="IW774_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1152707.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            38061..38831
                     /locus_tag="IW774_000328"
     CDS             38061..38831
                     /locus_tag="IW774_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1152708.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            39110..39964
                     /gene="aroE"
                     /locus_tag="IW774_000329"
     CDS             39110..39964
                     /gene="aroE"
                     /locus_tag="IW774_000329"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1152709.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            40142..41305
                     /locus_tag="IW774_000330"
     CDS             40142..41305
                     /locus_tag="IW774_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1152710.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            41422..41832
                     /locus_tag="IW774_000331"
     CDS             41422..41832
                     /locus_tag="IW774_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1152711.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            42006..44234
                     /gene="topB"
                     /locus_tag="IW774_000332"
     CDS             42006..44234
                     /gene="topB"
                     /locus_tag="IW774_000332"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1152712.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            44372..44689
                     /locus_tag="IW774_000333"
     CDS             44372..44689
                     /locus_tag="IW774_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152713.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            44849..45085
                     /locus_tag="IW774_000334"
     CDS             44849..45085
                     /locus_tag="IW774_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1152714.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            45082..45480
                     /locus_tag="IW774_000335"
     CDS             45082..45480
                     /locus_tag="IW774_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1152715.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(46147..47091)
                     /locus_tag="IW774_000336"
     CDS             complement(46147..47091)
                     /locus_tag="IW774_000336"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1152716.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(47387..>47530)
                     /locus_tag="IW774_000337"
     CDS             complement(47387..>47530)
                     /locus_tag="IW774_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1152717.1"
                     /translation="FALELIQETSFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWR
                     NKH"
BASE COUNT        15569 a         8249 c        10173 g        13539 t
ORIGIN      
        1 agcttctcgt gtttgatgaa tattgtaact tgtgattcgg aattggcagg agaacaccgt
       61 ttattcggaa gaataggact gtgacaagac ttttgtcaca gtcctttttt cgaattcaac
      121 aagtaaaact tttaagaaat attcagagag taaaattaaa aattgctgat aaacgaagat
      181 ggaaatttta tgtattttta ttggtctatg caataaataa attaaatatt cagaatacaa
      241 ttgattttaa cgaaagggct tctatcaatg gttgtgttgt atttatctgc ccaggtagag
      301 taaaatagat cattatctaa aaggtaattg ttcatactat ataagtcatt tgaaagtaaa
      361 aagatactgt aaataaaaaa tgtggttaaa taattctcat aatcagtgta agcgttatca
      421 aaaatatagc atacttgctt tgtagatgaa tgacacgata tatgtttggt atgttctgca
      481 agacagatga atatggaggt ggagacaatg tcttccaaag aactaacaat gtatttagag
      541 aaagaattgg atcaactgaa agaaaagggg ttatacaata cgatcgacat actagaaagt
      601 gaaaatggtg cttgtatcat cgtagacgga aaaaagatga tcaatctagc atccaacaat
      661 tatcttggat ttgccaatcg cgaagaattg aaaaaagcat gtattgaagc aaccgaaact
      721 tatggtgtag gagcaggagc agtccgaacg atcaatggtt cgctgaaaat ccatcagcaa
      781 ttagaagaaa aaatcgcaga attcaaaggg acagaagcag caatcgcctt tcaatctgga
      841 ttcaactgca atatgggcgc tatctctgca gtaatgacaa aagaagatgc tatcttatcg
      901 gatgaattaa accatgcatc tattattgac ggttgccgct tatccggagc aaaaatcatc
      961 cgaattaaac atcaagatat gaaagacctt gagaaaaaag caaaggaagc gatagaaagt
     1021 aagaaatata aaaaaatcat gtatatcaca gatggtgttt tctccatgga tggagacatt
     1081 gcacgattgc cggaaattat tccgattgtt gaaaaatatg gactgatcac ctacgtcgat
     1141 gatgcgcacg gctcaggcgt aactggaaaa ggtgcaggaa ccgtcaagca ttttggatta
     1201 agcgacaaaa tcgatatgca aatgggcacg ttatcgaaag ctataggtgt tgttgggggt
     1261 tatgtagctg gatcgaaaac actaatcgac tggttgaaag ctagaagtcg gccattccta
     1321 ttttctactt cgctaactcc aggtgcagct gcagctgcat tggcttcgat tactttgatg
     1381 caagaacatc cggaacttgt cgaaaaagtt tgggaaaatg ctaattattt taaagaagaa
     1441 ttgaaaaaag taggctataa catcggtatg tctgaaacac ctattactcc agtcattttg
     1501 ggagacgaaa aagtgactca gactttctct aagaagctga tcgaacatgg gatttatgct
     1561 aaaccaatcg tttatccaac tgtgcctctt gggacaggca ggatcagaaa tatgccgact
     1621 gcggaacata cgagggaaat gcttgatgaa gcagtagcag tttatcaaaa aataggaaaa
     1681 gaaatggaaa ttatttaact aaatttagtt ttttcgtcta tttaaaatta gagaacatgt
     1741 tcacgcatat attgttaatc atctgaagat agtagtaaaa aagatcaggg aggatcatca
     1801 tgaaaaaagt attggtgaca ggatgtttag gacaaattgg ttcagagttg atcacacggc
     1861 tacgtcaaga actaggcagt gaaaatgtca ttgcgacaga tatacgcatg cctgaagtca
     1921 ttgagaaaaa cgatatcttt gagatcctag atgtaatgga ttacgaaaaa atgcgcagca
     1981 tagcagaaca atatcaagtg gacacattga ttcatttggc tgctcttttg tcagctgttg
     2041 cagaagcaaa accacaatta gcctggaatt tgaacgtgac tgggctggtc aatgcattgg
     2101 aagtcgctag agaattccag ctgaaatttt ttacgccaag ttcaatcggc gcgtttggtc
     2161 caaatactcc aaaagatcat accccacaag atactcttca gcgtcctaca accatgtatg
     2221 gggtaacgaa ggtagctgga gaactactat gtgattatta ttatgtgaaa tatggcgtag
     2281 atactcgtgg tgtccgtttc cctggattga tttcttatgc aaccttgcca ggaggcggta
     2341 cgacagacta cgcagtcgat atttattatt cagcaatcaa agaaggaaag tacactagtt
     2401 tcattgatca aggtacatca atggatatga tgtatatgcc agatgcgatc gatgccatta
     2461 taaaactcat gaatgcagat cctgatcgat tggttcatcg aaatgccttc aatatatcgg
     2521 cgatgtcttt tgacccggaa gagatcaaag catctattca aaaaatcata ccagattttg
     2581 aaatggatta tgatgtggat cctgtacgtc agtcaatagc agaatcttgg ccaaattcac
     2641 tagatgtttc ttgtgcgcaa gaggaatggg gcttttcgcc gaaatacgac ttagatgtga
     2701 tgacaaagga tatgttggaa aaactgaaag aaaaattggt agagacggta taaaacaaga
     2761 accacatcca aaaagttttt tatacttttg ggattggcta tggtatgaaa ggggacaagg
     2821 gatggaagaa tatcaagcca cgccagtaaa agaagtaaaa atagaaaatg aattaaaacg
     2881 tacgatggga ttttttactg ctttatcgac tgttatgggc acagtgatcg gtgcaggggt
     2941 attttttaaa gccgcaagcg tagcagaagt tacaggatcc gccagtttgc atatgttttc
     3001 ttggtttctc ggtggtatga tttctgtctg tgcaggacta acaggagctg aactggcagc
     3061 tgctattcca gaaacaggag ggatgattaa atatatcgaa cggatttacg gaaatacagc
     3121 agctttttta ttgggctggg cgcaagtcgt gatttatttt ccagcaaatg tagcagcctt
     3181 gtcgatcatt ttcggtacac agttcgtcaa tctatttggt ctttcccagt caatgattgt
     3241 tcccgtagct gttacagctg ctgtatcgat cctgttgatc aattttttag gttctaaagc
     3301 tggaggggct tttcagtcga ttacgcttgt ctgcaagctt attcctttgt ttgtgatcgt
     3361 cattttcggt ttgttccgac aagaaggagt agacttccaa ctgtttccga tccaagcagg
     3421 agaaaatctt tcatttttct cagccttggg agcgggcttg ctagcaacga tgttcgcata
     3481 cgacggttgg atacacgtag gaaatatttc gggagaattg aaaaagccgg cgaaagattt
     3541 gccaaaagca atttctttag gaatcatcgg aatcatgatc gtttatttat tagtaaatgc
     3601 ggtgttctta agaaccgcat ctatcgacgg agtcgcagga aacagcaatg cggcaagtga
     3661 cgttgcaaaa atgatttttg gcggcttcgg tggaagactg gtcacagttg gtatcttgat
     3721 ttctgtttat ggaaccatca atggctatac ccttactgga atgcgtctcc cttatgttat
     3781 ggcaaaagaa aacaacctgc cttttagcaa gctctttgct aaacttcatg ataaaactaa
     3841 agttcctgta gccgcaggta tcttagagct agtcatcgct attggcatga tgatgatcgg
     3901 tggttttgat acactaacgg atatgctgat ctttgtcatt tggatttttt atacgatggt
     3961 ctttgttggt gtcattcttc ttcgtaaaaa agaacctgat ttgtttcgac catacaaagt
     4021 tccaatgtat ccgtttattc cactagtagc aatcattgga gggactttta tcgtatcaag
     4081 cacgcttatc actcagacct ttttagcttc gatggggata gtattgacac ttgctggtat
     4141 cccgatttat ctctatttaa aacgtagata gctaaaacat tcgaatttta ttagaactgc
     4201 acttctctgc caaacaaaat attttattta aatgaggagt acgtggaaga aatgtcaaaa
     4261 gtactcgtta attttgatca cgtaaatgtc gttttgtcta cgatgaaata gcaaagtaaa
     4321 agacagaaat cgggctcaac cgatttctgt cttttacttt gatttgcgta actgaactat
     4381 tcggcgtacg ccgcgataaa tatttaataa aaccaagcaa ccagaaatga aaatgggaag
     4441 ccaaatcaga tagacagggt agccaagcac tttagacaaa gcatgtggag tcattttatc
     4501 catttcaaga agtgcttgct gccaagtctg aagattgaag actagcatga aacccgaaat
     4561 aatcaggaga ataaatccta aataatgaat tgtagttctt tttcttacat aagcgatcgt
     4621 agcataaagg aacagtaaaa aaataggtac gccaaaaaca aagtacatat tcagcatctc
     4681 gacaccccct ttgtgtttat cttactttac tttgaaagaa atgagaaata atagcctcgt
     4741 tacttgcttg aaacagcaaa ggactgctaa gataagagag atacaaagaa tcaaggagga
     4801 gtcatatggc aagttatatc gaaccgattc gttttgcgct gattgttttt ccatttttag
     4861 ccttagcgat ctcatctgtt ttttttattt atgaatatcg aaagtatgga acatttctag
     4921 taacaagggc agttattttg tattctttcg ttttttattt attgtgtgcg tattttttgg
     4981 tgatcttacc acttccggca aaggagacgg ttgcccaaat gacagggcca aagatggagc
     5041 tgcatttatt tgcttcatgg caacatttca gagacaacac ggtattggtc ttgtctgaac
     5101 ctagtaccta cctgccggca atgaagcaga gtgtattttt ggaaccagct tttaatgttt
     5161 tattactcat accatttggc atttatttaa gatattattt ccggctttct tttatgaaaa
     5221 cgatgattgc tagtttctgc ctttctctat tttttgaatt gacgcagcta agtggacttt
     5281 attttatcta cccaagacca taccgcttat ttgatgtgaa tgatttagtg acaaatacat
     5341 tgggtggaat cattggtttt gtcgttacgc cgttatttac atttatgctt cctacaagaa
     5401 gacgaatgga tgaactttct tatgaaaaag gaaagactgt ctcattgact cgacgcttgg
     5461 tcgcttattt gatcgactgg acatttttat ctcttattaa tttggtagcg gcgattttct
     5521 ttaaaattgc gttcgattca ggggcagtta cttctcatat ctggtggctt ttattagaag
     5581 taggggtata ctttatgatt gtaccgttta taaccaatgg atggacactt ggcaaaaaaa
     5641 ttgtgcgtat ccgtttggtg gaagaagacc gcgaacgaat ttctttaaaa gcattgatga
     5701 tccgctacgg gtatctgtac tttctttttt atggtttaag ctggttgggg cagttgcttc
     5761 agcaaatcat gaattcgaca acgaatacgg tgttgcagat gagtgcagta ttcatttctc
     5821 tcggtgtctt gttactacaa ggtattttta ttttaaatat cttgctatca tttttgagaa
     5881 aaaaacggat tctgttttat gaaaaagcta gtcacacata tactgttagc acgattggcc
     5941 aaacaaagga tgatgattct cgaaattagc tgagcaaaaa cgctttgtct gtgttataat
     6001 gccaaagtcg tgataattta aagagaagag gtgcacgaat gaataatttt gatatattcc
     6061 gcttcaaacc gtttatcaat gaagcgttaa ccgcaaaggg cttcaaacag cccacagagg
     6121 tccaggaacg tttgattcct ttaattgcaa aaggaaaaaa tatcattggg caatctcaaa
     6181 ctggtacagg aaaaacacat acgtttttgt tgcctttgtt tgaaaaaatc aatgcagaaa
     6241 aagaggaagt acaagttgtt attacagctc caagtaggga actagctgca cagatctatg
     6301 aagcagctac acaaatcgct aaattttcta caccggagat ccgtgtctcg aattttgtcg
     6361 gcggtacaga caaacagcgt caattaaatc gtctgcatca ccaacagcca caggttgtag
     6421 taggcacgcc tggtagaata ttagacatga tgaatgaaca agcgctgaag attcacaccg
     6481 catttgcatt tgttgtcgat gaagcggata tgacattgga tatgggtttt ttaaatgaag
     6541 tagaccaaat tgcaagtcga ttaccagaaa agctgcagtt tttagttttt tctgccacta
     6601 ttccagaaaa acttcgtcca ttcttacgaa aatacatgga aaatccaatc attgaagaaa
     6661 tcaaaccaaa agcagttatt tctgaagaca ttgaaaattg gctgatttca acaaaaggta
     6721 aagatgagaa tcaaatgatt tatcaactgc tgacaattgg tcatccttat ttagcaatcg
     6781 tatttgccaa tacaaaacag catgtggatg aaattgccga ttacttgaaa aaccaaggct
     6841 tgaaagtagc aaagattcat ggagacatca ccccaagaga gagaaaacga gtgatgcgac
     6901 aagtacagaa tctggagtat caatatgttg tagcgactga tttagctgct agagggatcg
     6961 atatcgaagg tgtctctcat gttattaatg cagaaattcc agaagatcta gacttcttta
     7021 tccatcgtgt cggacgtact ggacgtaatg gcttaaaagg aacggccatt acgttatatt
     7081 cgccaaatga tgagcaagcc attgatgcga tagaaaaatt gggcgtctca tttgaaccaa
     7141 aagaaatcaa aaacggggaa atcgtcaaaa catacgatcg taatagacgt gccaaacgag
     7201 aaaaatcaaa agatgtatta gatcccacgc tgattggtct tgtgaagaag aaaaagaaaa
     7261 agatcaagcc tggttataag aaaaaaatcg attgggcaat tgcagaaaaa aataaaaaag
     7321 aacgcaaaat cgaacgcaga cagcaaagcc gtactgctcg taaaaataag aaaaattcac
     7381 ataaataaaa aacaagaggt tgtgacaaaa ggcgtgtcac aatctcttgt ttttaagcta
     7441 gacttttttt cattccgata caaagtactt catcttcaat aaaaggttct gtgcaagtat
     7501 gatacccttg tttttcataa aaatctacag ccgtcatttc agctactaca taactagtct
     7561 gacaaccttc tgaaaccgcc cgtttttcag cagcttcaag caactgaccg cctaaccctt
     7621 ttcctcggta ttcttttgct acgcaaaatc gttccggctt gaagcaagta ggtgttttct
     7681 tttcataacg caaagtagct actggtcttt tttgatcaag aaatagataa taggtttgtt
     7741 gcgggatatc ttttccgtcg aattctagtt ctgcaggaat cccttgttct aatacaaaaa
     7801 cttgatatct caaaaagcag ctagctgcgc gtatccattc atccgctcca actatttttt
     7861 tcatcatatt cctccttgtc atgaatagca gagatgtatt gattccttta ttcttatcgt
     7921 accaaaaatc gtggagtatg accaaaagga aatgaaaata aaaattgaga tattgacagt
     7981 gatcaataga atgcgtatac taaataagta cgaaaaggat atgatttgtt ttttaagact
     8041 cacagaaaat tctttcttgg ctgaaaaaag aatagtcact aatcaaattg ctcccttttt
     8101 ctattttggt agaaataccg acgcagctca gcgttatcga gtttaagagg taatgattac
     8161 acatcattgc aatcaaggtg gtaccgcgag ttttcgtcct tggctgtgat ggtgtttttt
     8221 tgtatttaat ttatagaaag caggtaatga catgaaacaa ttaagtagtg cacaagttcg
     8281 acaaatgttt cttgatttct tccaatctaa aggacatacg attgaaccta gtgcgtcttt
     8341 gatccccgtg aacgatccaa ccttattgtg gatcaactct ggtgttgcaa cattgaaaaa
     8401 atattttgat ggttcagttg tgccagaaaa tccaagaatc acaaatgccc aaaaatctat
     8461 tcgaacaaat gatatcgaaa atgtcggtaa aacagcacgg caccatacga tgtttgagat
     8521 gctgggtaat ttttcgattg gtgactattt caaaaatgaa gccatccact gggcatggga
     8581 atttttgact gatccaaaat ggatggcatt cgatccagaa aaactttatg taacagttta
     8641 tccaaaagat acagaagcaa aacgcatctg gcatgaagaa gttggcctgc cactagatca
     8701 cattattgat gtagaagaca acttctggga tatcggtgca ggcccatgtg gtccggatac
     8761 ggaaattttc tatgaccgtg gagaagctta taacgattta gcagaagacg atcctgaaaa
     8821 ctatccagga ggagaaaatg aacgttattt agaaatctgg aacttagtat tttcagaatt
     8881 caaccataag cctgataaca catttgaacc acttcctcat aaaaacattg atacggggat
     8941 gggattggaa cgcatggtct caatcgttca agatgcgtca acgaactttg agacagactt
     9001 gtttatgcct attatccacg ccgtagaaaa gctaagcaaa caagtaaaat atggtgaaaa
     9061 cccagtaaca gatatttctt acaaagtaat cgcagatcat attcgcgccc tttcatttgc
     9121 tattggcgac ggtgcattgc catcgaatga agggagaggg tatgtcttac gccgtttgct
     9181 tcgtcgtgct gtcatgcatg ggaaaaaatt aggtattgaa caagcattct tatatcgttt
     9241 agtccctgtt gttggggaaa tcatggtaag ttattatcct gaagtacttc aacaaaaaga
     9301 atttattgaa aaagttatcc gtacagaaga agaacgtttc aatgaaacaa tcaatgaagg
     9361 tttggaaatc ttaaatcaag tgatccaaga agtaaaaaac gaaaacggga cgactctagc
     9421 aggaaaagac atcttcaaac tatacgacac atatggcttc ccggtagaat taacggaaga
     9481 ggtggcagaa gatgaaggac tgaaagttga tcatgctggt tttgaaaaag aaatggatgc
     9541 acaacgcgaa cgtgctcgct ctgcccgcag tactgaaaaa tcaatggggg tacaatctgc
     9601 tttattgaca gatatcaaag tagaaagtaa attcattgga tacacagaac aagaatcaga
     9661 tagtcaatta ttggtcatcg ttaaaggtga gtctcttttg aatgaagtcg cagaaggtga
     9721 agctcaaatg atctttgatc aaacaccttt ctatgcagaa atgggtgggc aagtagcgga
     9781 tcaaggatgg atcatgaatg aagtaggcga aatcgtagca gttgttacag atgttcaaaa
     9841 agcgcctaat ggtcaattta tgcacaccgt agaagtaaaa gcaccaatga gtgaaggaca
     9901 aacctatcat ctattgatcg atcaatcgat gcgcaatcgg atcatcaaaa accatacagc
     9961 aacccatcta ctgcacaagg cattgaaaga agtacttggc gagcatgcaa atcaagctgg
    10021 ttctttagtc gctcctggac atttacgatt cgactttacc cactttggtc aaatcacttc
    10081 agatgaatta gctgaaatgg aaagaatcgt caacgaaaaa atctgggaag ctatcccagt
    10141 tgaaacaatt gaaacagata tcgatacagc caaaaacatg ggtgcaatgg ctttgtttgg
    10201 tgaaaaatac ggacatgacg ttcgagtagt aaatattggc ggctggtcaa tcgaactgtg
    10261 cggaggcaca catgtagcaa atacagaaaa catcggaatc ttcaaaatcg tttctgaatc
    10321 aggaattggt gcaggcgtgc gacgaatcga ggctgtaaca agtaaagaag cgtacgaact
    10381 acttgaagaa gaagaaaaac aactaaaaca agtagcaaca attgttaaat cacctcaatt
    10441 gaaagaagtt gtttcaaaag cggaacaatt gcaacagcaa gtaagagagc tacaaaagga
    10501 aaatgaacaa ttagctagca agttagcgaa tcaacaagct ggtgatgtat tcaaagatcc
    10561 agaaacgatc aatggaaaaa caatcattgc agcacaagta aaagtgaaag atatgaatca
    10621 gctgcgtcaa ttagctgacc aatggaaaca aaaaaatatt tcagatattc ttgttttagc
    10681 aaacgaacaa gaaggaaaag tcagtctgct agcggcaatg acgaaagaat caaatgaagc
    10741 tggactaaaa gctggcgact tgattaaagc aatcgcaccg aaagtaggcg gtggcggtgg
    10801 tggacgtcca gacatggcac aagctggcgg gaaaaatcct gctggtattc cagatgcact
    10861 taaagctgcg aaagaatggg ttgcgcaaca atagggcgtt tacgtttgac agaaaaacaa
    10921 aagttcaaac attgaacgaa acatgattat gggggcgaag acaaacaagt cttgccctca
    10981 tacttttttc aattggatgg cgcatctaat caaatggtcg gattcggatg gtctttagtt
    11041 gacgaaagtg acagaaattt ttacgattag tataaataaa aaggggtgaa aaaatgaata
    11101 gtagaaataa gacctttcga ctagtcttac gtgcgatctt gttagcaatc atcatcgtac
    11161 aagcgatggt tccttggctg gggtttattc cattaggatt cattagttta acaattatcc
    11221 atatcacagt aattattgct gcagtagtat taggcccaaa agatggcatg gtgattggct
    11281 tgttttgggg aatcgctaca attgttcgtg cctatgccat gccaacaacg ccatttgata
    11341 cattagtgtt cacgaatccg attatctcgg tagttcccag agtacttgtt ggcttagtag
    11401 caggcttggt tttccactgg atttatcaaa aaaataaatc aataacgatc agctcagcct
    11461 ttgcaggggt actgggttca ttggtcaata cagtcttagt gttaggattc atgggattat
    11521 tttatacagg tgcaacggca gatgcctatg gtgtagactc tgctctttta tttaaaacct
    11581 tagcaggggt tgcagcaatc aatgggatac cagaagcaat aggtgcggga atcataacac
    11641 cattactagc aaaagcgtta tttgcagcaa caccgttgaa accagaataa tgaccatctt
    11701 ttaatagtaa taggacgcac gatagataga aaaaggaaga gaggagagga aaacaatcgt
    11761 cctctccatc tttttacctg agcaacaagc aaactagaag gggtaaaaaa cttggctaag
    11821 aaacaagaag aaaaaaagaa acaaataccg caacagcgga caaaaaaagg agtactagcg
    11881 ggtatcttag gattggttac gaatctgatc ttgtttgtct ctaagctaat gatcgggctt
    11941 ttttcaggaa gtgtctccat tatggctgat gcagtgaaca gtctatcaga tactgcttca
    12001 tccatattga ctttgatcgg atttaaaatt gctgcaaagc cagcagatca ggaacatcct
    12061 tatggacacg aacgcttcga atatatcagt gggttatttg tttcgatcat cattacttat
    12121 gtaggatttc aatttttaga gtcttctgct gaaaaaatca tccatccaga aaatgtctct
    12181 ttaacaccca tcgtcttttt tgtcctaatc atttccatct ttttgaaatt tgcacaaggc
    12241 aaaatgtatc aggttattgc gcaatccatt gattctgaaa cattgagagc tacagcaaca
    12301 gatagttata atgatgtctt tactacactg gctgtattgc tttcagcagc aatcgaatgg
    12361 gtaactggat ggcgtattga tggatatata ggttttgctt tagcattata tatcctttat
    12421 agtggattga cgatgcttcg aaattttgta tacgaactat taggaagtcg cccaacgcca
    12481 gcagagattg aagaaatgga agagcattta aatgcttgcg atgatatact tggctatcat
    12541 gatttattgg tgcataacta tggacccaat aaacgctttg catcagtcca catcgaagta
    12601 gatgaatcaa tgaatttgaa tgtggctcat aagattattg atctgataga aaaagacttt
    12661 aaaaagtctt tggatgtgga acttgtctgt cacttggatc caatgcctgt tgataacgaa
    12721 aaatactata atatcttgcg gcagttaaag caaattcttc ttgaaacagg tacagggctt
    12781 aatattcatg attttagagt aattcatcaa ggaaaaacgc tgcaatttga tgtagtggtc
    12841 cctgagaaat tccagtttgt tgatacagaa ctagaaagaa tcttacgtag tcaaatagaa
    12901 gaaaaaatcg gagattacca attggaaatt acgtttgatc acaattatct tttatattaa
    12961 atgagaaact ataaaaagac tccttaaagt ttttaattta aagagtcttt ttttctttta
    13021 attgtttatg gagagcctga cgtgcaagat tatctgctcc tttatttttg ttttcgggca
    13081 accattgcag gatcaataaa gaaaattcgg atagaagttc ttggattctt tgaaggtagg
    13141 gaaggaagag ctcgttcttc gttgcgtttt tgtcgaccgt agaaactagt gttttgctgt
    13201 ctgaaaaaca gaaaatcgtt tcattctgta agtcgttttt ctttagaaat tctaaagttt
    13261 ttaaaaagac ttcgaactct gcttgatggt ttgtccctat agaaagcggg atagatagct
    13321 gttcttgttt accttgatag acaatcaaga ttccacctcc actaggacca gggttgcctt
    13381 ttgtagaggc atcaatgtat acttttaaca taaaaagaga ttctcctttt tgttcaaact
    13441 aatgttatgg taagattata tcattactga atcggtgaga ggttagagaa tgaaaaaaaa
    13501 tattcgatgg cagcctgaac tggcacatac gatcatctat tggtcattga ccttgatcat
    13561 tctgtttttt agtctgattc tttctttaga aaacacaggt ccttattgga aaagcaattt
    13621 tgttttaggt atttttcttg tttttctatt tctaggtttg cggcgttctt ttattttaaa
    13681 atcaaactct gtgattatac gttatgctgc tttttggaaa gaccgagaaa tatctgtcgg
    13741 acagattcga tctttgacgc caactaatac tggggtgcaa atgatgttgc aagatgagaa
    13801 agccccatct gttttcatga tgcgaaaaaa agatcaagta gtcctttttg atcattttga
    13861 aaaaaacgga cccaaatcag aatcaattcc ttatccttat acgaaaaaaa gaaaaagcta
    13921 attttaaaaa tcagaaccta aaaaaacgaa tttttacagg aaaagaaaag agtgagacgt
    13981 acggttcatc ccgtttagcg tctcactcta tttgtttatc tattcatgca tactagtatc
    14041 ggtagtctga acgttgcctt tcttgacatg ggcagcttgt gttcctcgat tcccttcgat
    14101 gatctcaaat tcgaccactt gattttcttc caaagttttg aatccctctt cttcaatcgc
    14161 agtaaaatgt acaaaaattt cttctgtgtc atcataactg ataaagccgt atcctttttt
    14221 gttgtcaaac cattttacaa ttcctgtggt caaagcactc acgacctttc taaagtctga
    14281 tcatagcctt cattataaag agtatttata agtacgtcaa ataaattaaa aacctgtaat
    14341 tttatgtttt ttttctgaat aaaaaaaggt ataattggca tatcttaaca aagaagatat
    14401 tttgttgaat aaagttcgtg aaaaggggta atcaaatgaa gaatgatctg caaatttcac
    14461 aagaaacaga gctagaaccg attgttgaag tggctagtaa attagatctt aaggaagacg
    14521 atttggaact gtatgggaaa tataaggcga aaatcaattt ttctggaatc aatcgatcaa
    14581 aggataacgc tgaggggcat ttgatcttag ttacttccat caatccaaca ccagctggcg
    14641 aaggaaagtc aacgatcact gtagggttag gagacgcgct caataaaatc aataaaaaat
    14701 ctgttattgc attgcgagaa ccctcacttg gtcctgtgat gggaatcaaa ggtggtgctg
    14761 caggcggagg atatgcacag gtattgccta tggaagatat caacttgcat tttactggtg
    14821 acatgcatgc tatcacaaca gctaataatg cattatctgc tcttttagat aatcatattc
    14881 atcaaggaaa tgaattgaac atcgatgccc gccgagtgat ttggaaacgg gtagtcgatc
    14941 tcaatgatcg tgaactgcgt aaagtaattg ttggcttggg tgggccaatc caaggtgttc
    15001 caagagaaga tgggtttgat atcacagttg ccagcgaaat catggctatc ttgtgtcttg
    15061 cggcagacct tgaagattta aaggctcgtc ttgcaaggat cgttgtcggc tatacatttg
    15121 accgtcagcc tgtgactgct ggagacttga aagcagaagg cgctctggca ttactgctga
    15181 aagatgcaat caagccgaat cttgtacaaa caatttacgg aacaccagct tttgtccacg
    15241 ggggaccgtt tgccaatatc gctcatggct gcaatagtat tttagctacc aagacagcat
    15301 tgagattagc agattacgtt gtcacagagg caggatttgg tgcagatctt ggcggagaga
    15361 aatttttaga tatcaaagta cctaacttga aaaaagcgcc ggatgctgtg gtgattgtcg
    15421 ccactgtccg tgcattgaaa atgcatggcg gaatgaaaaa agatgaactt aaaaatgaga
    15481 atcttgatgc tttgaaagta ggttttgcaa atttaaagcg ccatatacgt aatatggaac
    15541 aatatcagct acctgtgatc gtcgcaatca atgaattcgt aacagataca gacaatgaac
    15601 tcacactgtt ggagcacttg tgtgaaaatc aggggattct tgccaagaga gcgagtgtat
    15661 gggcagatgg agcagaaggc ggcgttgatt tagcaaaagc tgtagtacga ttgattgata
    15721 gaaaagaagc taattacaag ccattatacc gcctagaaga aacgatacaa gaaaagacag
    15781 aaacaatcgt caagaaaatt tacggaggaa atggtgttgt tttcagcaaa aaagcgcaaa
    15841 aacaaataga agagtttacg aagaatggat gggacaaact gccgatttgt atggcaaaaa
    15901 cacagtattc attttctgat gatccttcac tgctaggtgc accggaagat ttcaccatca
    15961 cgatccgcga aatcatccca aaattagggg caggattttt agtagccttg actggagatg
    16021 ttatgacgat gcctggtttg cctaaaaaac ctgctgcatt gaatatggac gtgacaaatg
    16081 acggagaagt gttgggattg ttttaagctt gattataatc taccatcgaa ggagctgtat
    16141 tttgactaca gcttcttttt ttagacgata gggaattaat tttgtttaca ctaggatcat
    16201 tctttttctt tattgtattt tgagctatac taaagcataa taggagggta tgatgaaaac
    16261 gacatttata agaaaaaccg gtttttacgg tatgggtagc tcgattcagc taagaatcaa
    16321 caatgaaaaa cgcagtgttc tcagtcatga acaaaagata acgcttgatc tggaagtgcc
    16381 ctttacgatg caggtcacct tttattggct aaaaagcccc gtttatacag tgaaagaagc
    16441 aaagcctgct tatgtgatca caatgaatta cttgattctt caaatctatc cgtttttatt
    16501 tctagcaact gggttggggg ccgttttcct acaaaattta tttttcagtt tttttgcagt
    16561 tattgtgttg attgcgttct ttttctatat aaaaaaccgg gcatatttga tcaaggagga
    16621 aaattatggc aaactctgat ctttttttaa tcagtttcaa tcgaatcgaa aaatggatga
    16681 gagacgagat gggaaatgca cgaaatatgg gatttaccga acttgtcaga cgcctagctc
    16741 aaagaaaaca attaatgatc agaaaatacg aagatgatct tttacagctt gctcagttaa
    16801 ggaatgcgat cgtccatgat cggatcgcag tcgattttgt cattgcagag ccaaatgatt
    16861 ggacagtcaa gcgaatccag ctgatcgaac aagaactgat tcagcctgaa accgtgctgc
    16921 ctcgatttgc aaaacgtgta acaggttttg aacaagatct tccgttactt gaactgttaa
    16981 agatagtagc agaaaaaagg tattcacaat ttccgttata caataaagga aaatttgtag
    17041 cattgattac tttacgtaat atcgggtttt ggctagcaaa agaaagccaa aaaggtccgg
    17101 ttgatttaac aaataaacga gcgttggatt tgatcatcca aaacggaaag tacacaaatt
    17161 atcattttgt tccagctaaa acacatattt acgaggttga agcgatgttt cgtgagcaag
    17221 ggactttgga agccgtactt atcactaaag atgggaatcc cgatggtaat ctgctcggga
    17281 tcgtacgacc tcgtgatatt tataaacaaa tagaaaagga ttgatttttt gttaattatt
    17341 tattggatat tcagcgcttt gctagtagga gtggatcagt tggtcaaatg gtggatcact
    17401 gataatctag cgcttggaga gacgaaaaat cttataccag gtattttgtc actgaatcat
    17461 attcgaaaca ctggagccgc atggagtatg ctagaaggga agatgtggtt ctttactata
    17521 gtgacactca ttgcagtcgt tgtgattctc acattgatga ttaaaaatcg tgaaaaaggt
    17581 aatcgctggt tcatgattgg attgagtttg attctagctg gtgcgattgg caattttatc
    17641 gaccgtattc gtcttggcta tgtagtagat atgttccaga cagattttat gagctttccg
    17701 atttttaacg tagcagatgt gacacttgta tgtggagtga tttgtatgtt aatttatatt
    17761 attttagatg aaaaggatca aagaaaaaaa tgacagaact taaaacgacg attcaccatg
    17821 aatctggtag gatcgataaa gtattgacta atttattcac cgattacagt cgttcgcaaa
    17881 ttcagctgtg gctgaaaaat ggagcggttt ctgtcaatgg acagccagtc aaagcaaatt
    17941 acaaagtaaa acaaggagac gagattactg tcttgatccc cgaaccagaa acattagatg
    18001 tggtcccaga aaatatcccg ttgacgatcg tttacgaaga tgaagatgta gcggtgataa
    18061 acaaacctca aggaatggtc gttcatcctt cagcaggtca tacgcatggc acaatggtca
    18121 atgcgctgat gtaccatatc aaggatcttt ctacgatcaa cggcgttatt cgtccaggta
    18181 ttgttcaccg aatcgacaaa gatacttccg gactgctaat ggttgccaaa aatgaccagg
    18241 cacacgaatc attagctcag caattaaaag ataagacctc tttgcgcaaa tatgttgctt
    18301 tagtccatgg agagattcct cacgaaaaag gaaccatcaa tgcgccaatc ggccgttcga
    18361 aggaaaaccg gaaaatgcag gcgataagag aagatgggaa gtcggctgtc acacatttta
    18421 ctgtattgga acgtttcgaa ggatttactc tggttgaatt aaaactagaa accggaagaa
    18481 cacaccagat ccgtgtgcat atgaagtata tcggttatcc gttagctgga gatccagtat
    18541 acggaccgaa gaaaactttg aaaggcaatg gtcagtttct ccatgcgcaa atgttaggtt
    18601 ttacgcaccc gactacaaac gaaaagatga tctttgaagc acctcttcca gaagtttttg
    18661 aaaaaacttt agaaaaatta agaaaaaaca ttgcatttta atttagataa cggtatagta
    18721 attaacaacg aaagcataaa acttaataga attcctttaa gtttagtccc gtgaggctaa
    18781 gaagggtggg agcaaggata cgtatggtgc gccataagta gattgctcat tgacatagta
    18841 tagctatcag tgtatcagta tacgctcagt ctaaacattc ctcctgtcct cttagggcag
    18901 gagttttttt atgcaaaaaa gtagggggaa tgaaaatgca agcaaaagaa gtagtagatc
    18961 aggtaaccat gaagcgagca ttgactcgaa ttacttatga gattatcgag agaaaccata
    19021 gtatccaaga tatcgtattg gtaggtatca aaacacgagg aatctacatt gcatcaagaa
    19081 ttgccgaacg cctgaagcag ctagaagata tcgatattcc tgtaggagaa ctggatatca
    19141 ctttataccg agatgacaaa aaagaaaatc cagaagaacc ggaacttcat tcttctgaca
    19201 tcccagtttc cttagaagga aaagaagtta ttttgattga tgatgttttg tatacaggac
    19261 gaacgattcg agcagcaatg gacgcagtaa tggattttgg cagaccaaga aaaatttcat
    19321 tagcagtcct ggtcgatcgt gggcatcgag aattgccaat ccgtgcagat tatgttggga
    19381 agaacattcc aacatctcgg gcggaagaga ttttagtaga gatgcaagag cttgatggac
    19441 aagatcgcat catgattttg aaagaggaag attagaggag agagaaacat gtcggagaaa
    19501 aaaggatttc ataatgatga cgtggtgtta gatatccacg atcgcccaac cccaggacat
    19561 tgggtaggat taagtttaca acacttgttc acgatgttcg gagcaaccgt gttagtcccc
    19621 atcttagtag gtatcgatcc aggtattgcg ctagtcagtt caggactagg tacattggtg
    19681 tacttattta ctactaaagg aaagatacct gcgtacttag gaagcagctt cgctttcatt
    19741 gcagcgatgc agcttttgat gaaaacagat ggttatccag cgattgcaca aggagcagtc
    19801 acaactggat tagtctattt gattgttgcg ctgatcatca aaaagatcgg ttcagcatgg
    19861 ctggataaaa tcttgcctcc aatcgtcgtc ggtccagtca tcatggttat cgggttggga
    19921 cttgcggcaa atgcagcaac aaatgctatg tataatcagg gcacgtatga tttcaagtat
    19981 attgctgtag cgctgatcac attaggatta acaattttct tcaacatgtg gctaagaggt
    20041 tttctcggat tgattccgat tttactaggt attattagcg gatacatcgt agccttgctg
    20101 tttggaatcg tggatactaa gccaatcgta gacgctgcct ggtttgctgt tcctaacttt
    20161 gagattccct ttgtacaata tgaaccaaag ctatatcttg gagcaatcac aacaatggca
    20221 cccatcgctt ttgtgacgat gacagagcat atcggccatc ttatggtatt gaataaacta
    20281 actaaacgta atttctttga agaaccgggt cttcataaga cgttgacagg tgatggatta
    20341 gcacaaatcg tggcagcctt tgtcggaggt cctcctgtaa caagttacgg tgaaaacatt
    20401 ggtgttctgg ctatcacacg tgtccatagc gtgtttgtga tcggtggagc tgcagtattt
    20461 gcagtcattt taggattcgt aggaaaatta agcgctttga ttttaagtat ccctggacca
    20521 gtcatctcag gaatcagttt tatcttattt ggagtaattg ccgcaagtgg aatgaagatt
    20581 ttaatcgaga acaaaatcga ttttgataaa aagaaaaatc tgttgattgc ttcagttatc
    20641 ttagtagtag gaattggcgg actcgtcttt gaagtaggca catttacatt gtcggcaatg
    20701 gcattagcaa ctgtacttgg catcatttta aatcttatct taccagaaac ttcacgaagc
    20761 gaagaaaact aaggaggaaa cattatgatc atcaaatcag aacgtatcag cttaaagcat
    20821 ttattgaccg ttgaggcact tactgatcaa gaagttatgg gcttgatccg cagagggcag
    20881 gaattcaagc aaggtgctaa ttggactccg caaaagcgcc agtactttgc tacaaacctc
    20941 tttttcgaaa atagtactcg cacacataaa agcttcgatg tagcggagaa aaaactaggg
    21001 ctagaagtga ttgaatttga agcaagcaca agctctgtcc aaaaagggga aacactatac
    21061 gatacagtac tgacgatgtc agcattaggc gtagacgtag cggttatccg tcatggtgat
    21121 gagaactatt atgacgaact gatacaaagt aaaacaatcc agtgttcgat tatcaatgga
    21181 ggagacggaa gcggacagca tccaacccaa tgcttgcttg atttgatgac catttatgaa
    21241 gaatttggtc attttgaagg tctgaaagtt gccatcgtag gtgatatcac tcactctcgc
    21301 gtagctaaat ccaatatgca gatgttaaaa cgtctaggcg cacaagtatt cttctcaggt
    21361 cctagagaat ggtacgacga tgaattcgaa gtgtacggac attatatgcc attggatgaa
    21421 ctactagatc aagttgatgt catgatgatg cttcgggtgc aacacgaacg ccatgatgga
    21481 aaagaaagtt tctcaaaaga aggctaccat caggaacatg ggttaacagt tgagcgtgcg
    21541 aagaagttgc aaaaacatgc aatcattatg cacccagcac cagttaaccg tgatgtagaa
    21601 ttagctgatt cattagtgga aggattacag tcgcggatcg ttcagcaaat gagcaatgga
    21661 gtattcgtca gaatggcaat cttagaagca gtgctaaatg gaaaagcgta attataggag
    21721 gatgaaaaaa tgaaaacact cattaaaaac ggtcagatca atacacgaaa aaatatgacg
    21781 acaccagcag aaatttggat agaagatggg cgaatcaaag caatcggtac aggattctca
    21841 gaagccgaat ttgatgaagt gtttgatgca aagggacaat tgatcacacc tggtttagtg
    21901 gacgtccatg ttcatttgag agagccagga tttacataca aggagacgat cgaagctggg
    21961 actcgctcag ctgctcgtgg cggtttcact actgtatgcg cgatgccgaa tttaaatcct
    22021 gtacctgata cggcagaaaa gctgagacaa gtatatgaca tcattagaaa agatgcagtt
    22081 gtgaaggtat tgcagtatgc gccaatcaca gaaaatctgc gaagtgagaa attagttgat
    22141 caagaagcac tgattgaaga aggtgctttt gctttcacaa atgacggtgt aggtgtccaa
    22201 acagctggga cgatgtatct ggctatgaaa gaagctgcaa aaaacaataa agccctagta
    22261 gcccatacag aagatgaatc tttattattt ggcggcgtta tgcacgctgg caagaaggca
    22321 gaagaacttg gtcttcccgg gatcttaagc gtaaccgagt cttctcaaat tgctagagat
    22381 ctgttgctag ctgaagcaac tggtgttcat taccatgttt gccatgtttc tactaaagaa
    22441 agtgtccgtg taatcagaga cgcaaaaaaa gcaggaatcc acgtaacagc agaagtttca
    22501 ccccatcatc ttattttgat cgacgaagat attcccgaag atttcggctt ttggaaaatg
    22561 aacccgccgc taagaggacg ggaggacaga gaagctttga tcgaaggctt gcttgacggt
    22621 acgatcgatt gcatcgctac agaccatgcc ccccatggat tagaagaaaa aagccaaagt
    22681 ttcatgaaat caccatttgg tatcgtagga agcgaaacag cgtttcaact gatctatact
    22741 cattttgtag aaactggtcg attcacgttg gaacaagtga tcaattggtt ggcagttaaa
    22801 ccggctgaga tttttggttt gaatgcagga acgctaacgg ttggtgcccc cgctgatgta
    22861 gcagtttttg atattacaca aacttgcacg atcgataaag aagacttttt atcaaaaggg
    22921 gagaatacac cttttatcgg ctggaaagta aaaggcgaaa cacaaatgac ttttgtcaac
    22981 ggaaaacttg tgtggcagaa gggagaataa aagtatggaa cgtttgctta tattagaaga
    23041 tggaacagtt ttcgaaggaa aggcttttgg tgcagatgct agtgtcttcg gagaaatcgt
    23101 ttttacaaca agtatgactg gttatcaaga aactatcact gatcaaagtt ttaacggtca
    23161 aatcattaca tttacctatc ctatggtggg taattatggg gtaaatagag atgattatga
    23221 atcgatttct ccaacatgca aaggagtagt agtaaaagaa catgcacgtg tagcttcaaa
    23281 ctggcgaaac caaatgacgc tagattcatt tttgaaacaa aaaggaattc ctggaatatc
    23341 aggtatcgat acgcgcgccc ttactcgcaa aatccgtgaa gtcggaacga tgaaagctag
    23401 tatagttgat gctggagatt ccttcgaaca tgcatttgat cagctgaaag caaccgttct
    23461 agcaaccaat caagtgcagc aagtctcaac taacaaacct tatccaagtc caggaacagg
    23521 tcggaaagtc attgtcattg acttcggctt gaaacatagt attttacgag aattgagtaa
    23581 aagaaactgt aacctcacag tacttcccta taacacagat gccgagacga ttttagcgct
    23641 atcaccagac ggagtcatgc tgacgaatgg acctggtgat ccaaaagatg tgccagaagc
    23701 aatcgaaatg atcaaaaaaa tccaaggaaa agtaccgatt tttggtattt gtctcggtca
    23761 ccagttattt gctttagcca acggtgcaga tacctataaa atgaaattcg gacatcgagg
    23821 attaaatcac ccggtacgag aagtagcgac cggaagaatt gattttactt ctcaaaatca
    23881 tggctatgca gtcgatgaag ccacgattga tccagaaaaa ctcatcgtaa ctcatgtaga
    23941 agtaaatgac ggaacagtag aaggagtcaa acacagagat tatcccgctt tcagtgtgca
    24001 gtttcatcca gatgctgccc ctggtccgca tgatgcacta catttatttg atgaatttat
    24061 ggaaatgatg gacgcaggaa aggagcagta aacagatgcc gaaacgtaca gatatcaaga
    24121 aaattatggt gattggatct ggtccgatca tcattggtca agccgcagaa tttgactacg
    24181 caggaacaca agcctgcctt gccttaaaag aagaaggata tgaagtggtt ttagtaaatt
    24241 ctaatcctgc gacgatcatg acggataaag aagtggcaga tcaagtatat atcgaaccga
    24301 tcacacttga atttgtatca aggattcttc gaaaagagcg tccagatgct ctccttccaa
    24361 ctttaggcgg acagacaggc ctcaatatgg caatggagct ggcagcatca ggtattttag
    24421 aagaattgaa catagaactt ctaggaacaa agctttctgc gatcgatcaa gcagaagatc
    24481 gtgatctatt caaacagttg atgaaagaat tgaatcagcc aatccccgag tctgaaatcg
    24541 tcaatacagt agaagaagct gtagattttg caaataaaat cggctatcct gtcatcgttc
    24601 gtccagcctt tactttaggc ggaactggtg gaggtatgtg tgaaaatgaa gaagaattac
    24661 gtatcgtatc agaaaatgga ttaagtcttt cccccgctac ccaatgtttg attgaacgaa
    24721 gtattgcagg ttttaaagaa attgaatatg aagtgatgag agattcggct gataatgcaa
    24781 tcgttgtctg caatatggaa aattttgacc cggtgggtat ccatacaggt gattctatcg
    24841 tatttgcccc tagtcaaaca ttgtctgacc atgaatatca aatgcttcgt gatgcttctc
    24901 tttcgattat tcgagcactt aagatcgagg gaggctgtaa tgtccaactc gctcttgatc
    24961 cacatagttt taattattat gtgattgaag tcaatccgcg tgtttctcgt tcatctgcgt
    25021 tagcaagtaa ggcgactggc tatccgatcg ctaagctagc tgctaaaata gcgcttggat
    25081 taacattaga cgaaatgaaa aatcctgtta caggaacgac ttttgcagaa tttgaacctg
    25141 cattagatta tgttgtagct aaaattcctc gctggccgtt tgataagttt gaaaacggag
    25201 aacggctttt aggcacgcag atgaaagcaa ctggtgaagt gatggctatc ggtcggaata
    25261 tcgaagagtc tttattgaaa gctgtgcgtt ctttagaaat cggtacacac catatcgaac
    25321 ttcctgagtt ggcagacatc acggacgatg acctaatcca aaaagtggtt cgagcacaag
    25381 atgatcgcct gttttatgta gctgaagcta ttagaagagg gtacaccata gaagaattag
    25441 cggatttgac aaaaatcgat ttattctttt tagataaact gttgcatatc gtagaaatcg
    25501 aacagcagtt gaaacagcag ccgttggatg acaagatatt gaaaacagcg aaacaaaacg
    25561 gttttacaga caaaaaagtg gcggagctgt gggaaataaa cgaagaagcc gttcgccaga
    25621 tgagaaaaga aaatgatttg attcctgtat ataagatggt tgacacttgt gcagcggaat
    25681 tcgaatcaaa aacaccttat ttttatagtt cctatgaata tgaaaatgaa agttcacgat
    25741 cagaaaagcc ctcaattcta gtactgggat ctggtcctat ccgtatcgga caaggggttg
    25801 aatttgacta tgcgactgtg cattctgtca aagcaatcca gcaggcagga tacgaagcaa
    25861 tcatcatgaa cagcaatcct gaaacagttt cgactgactt ttctatttcg gataaattat
    25921 attttgagcc attgaccttc gaagatgtga tgaatgtgat cgagctagaa cagccgatag
    25981 gagtaatcgt tcaatttggt ggtcagacag caatcaactt agccgaacct ttgactgctg
    26041 ctggtgtaaa aatcttaggg acaacgatcg aagacttgga tcgtgcggaa aatcgtgatt
    26101 tattcgagca agcgctgaaa gagttggata ttcctcaacc tttaggggat actgctacaa
    26161 gtaaagaaga agccgtggtt atcgcttcga gaattggtta tccagttctt gttcgtccaa
    26221 gctatgtatt aggcggacgt gcgatggaaa tcgttgaaaa ccagcgagat ttagaagatt
    26281 acatggaaca tgctgtgaaa gcatctccag aacatccagt actagtggac cgttacttgc
    26341 ttggtggtga atgcgaagtg gatgcgattt gtgacggtga aacagtactc atcccaggga
    26401 tcatggagca tattgagcgt gctggtgtcc attcagggga ttcaatggct gtgtatccac
    26461 ctcaaacatt atctgaatcg atcaagaaaa cgatcgaaga ttacacgatc cgcttagcat
    26521 taggtttgaa ttgtgtcggg atgatgaata tccagtttgt catccatgaa aatgaggttt
    26581 atgtgatcga agtcaatcct cgagcaagtc ggactgttcc attcttaagt aaggtgacag
    26641 gaattccaat ggcacaaatt gcgacgaaag ctattttggg agagaaacta aagacacttg
    26701 gttatgaaaa cggactttat ccagaaacaa aagaagttca tgttaaagca cctgtctttt
    26761 cttttacgaa attgcagaaa gttgatactt acttagggcc tgaaatgaaa tcgactggag
    26821 aagtaatggg atctgacaga caattagaca aagctttgta caaggcattc gaagcttctg
    26881 gattacactt gcctgaatat ggagccgtat tatttacgat tgcggacgaa acaaaagaag
    26941 aagcactaga attagctaaa cgttttacag aaattggcta tagtctgata ggaacgaaac
    27001 atacagctgc ttatttcgaa aaaaatggtc tggtcgttga aacagtagcg aaaatttctg
    27061 aggaagcagc agaaaaaaat gtagtcgact taataagaga tggtaaaaca caagtggttg
    27121 tcaatacgat tgataaagat cggggaaatg cttctaaaga cggtttcatc atccgaagag
    27181 aagcagttga acatggtatt ccattgttta cttctttaga cacagcagat gcgatcgttc
    27241 gagtgatgga atcccgtgct ttttcaacta accctattta acggcgagca catgttaaga
    27301 caaaaagacg aaaaaccgtt gcaggagggg acagatgaaa caagaaatga tggaaatcgt
    27361 tcagcaaaaa cagctggcac caaagatttt tgaaatgaca ttgaaaggtg atctagtaaa
    27421 agaaatgcag caaccaggac agttcttgca tattctcgtg ccgcgtgcag acttgctatt
    27481 acgccgaccg attagcttga atcatatcga ccacgaaaca aatacttgcc gtatcattta
    27541 tcgggtggaa ggtgagggga ctagagtctt ctctactctt tctgccggag accatttaga
    27601 tgtcatgggt cctttaggca atggttttga tttgtccaat cttcatacag gagacgaagt
    27661 atttataatc ggtggaggaa tcggtgtccc tcctctatat gagttatcaa aacagttagt
    27721 cgaaaaagga attaagccaa cgcatttttt aggttttgcc acacatgaag tcgtttatta
    27781 tgaaaaagaa tttaatcgtc taggagaaac aagaatcgct actgacgatg gaacatacgg
    27841 tatgcacgga aatgtcggta atttattgtt aggaacgaag acggaaccta cagctgtttt
    27901 tgcatgtggt tcaaacgggt tattgaagac agtggaacag ctgttttctt cgcatcaaaa
    27961 tgtccagtta tcattagaat cccgcatggc atgtgggata ggggcttgct atgcttgcgt
    28021 ttgtcatacg ccggaagatg aaacaggaac aaaaagtgtg aaagtttgtg acgaaggacc
    28081 gatttttaaa ataggagagg taattatatg aatgagaatc ctttagcggt caaacttcct
    28141 ggcctagaat taaaaaatcc tatcatgccg gcaagcggct gtttcggttt tggcaaagaa
    28201 tacggaaaat actatgattt gaatcagctt ggatcgatca tggtcaaggc aacgactcca
    28261 aatgcccgct ttggcaatcc cacgccaaga gtggcggaaa caccatctgg catgttgaat
    28321 gcaattggtt tgcaaaatcc tggtctagac gttgttatgc ataaacttct tccagaatta
    28381 gaggagtaca atgagttacc catcatcgca aatgttgctg gtgcttgtga agaagactat
    28441 gtagaagttt gtagcaaaat tggcgaagca ccaaatgtga aagcaatcga gctgaacatt
    28501 tcttgcccaa atgtcaaaca tggcggaatt gcttttggaa cagattctga tgttgctttt
    28561 caattaacac aagcagtaaa gaaagtttct tctgttcctg tctatgtcaa attgtcccca
    28621 aatgtaacag acatcgttcc aatcgctcag gcaatcgaag ctggaggggc agatgggttt
    28681 acgatgatca atactcttct tggtatgcgg attgacctta agacaagaaa accgatttta
    28741 gccaatcaaa caggtggact ctccggacca gcaatcaagc ctgtcgctat tcgtttgatt
    28801 caccaagtag caagtatatc cagccttcca atcattggga tgggcggcgt gcagacagtg
    28861 gatgatgttc ttgagatgtt tatggctgga gcaagtgctg tagctatagg gaccgcgaat
    28921 ttcaccgatc cgtatatctg tcctaaacta attaaagagc tccctgtgcg aatgagcgaa
    28981 ctaggcatcg aatcgctgga aagattaatc aaagaagtga gagaggaacg tgaacgatga
    29041 atcaactaga ccaacgacca attattgcat tagatttttc aactaggcaa gaggtagaag
    29101 actttcttcg atttttcccg aaagaagaaa aattgtttgt gaaaatcgga atggaattgt
    29161 tctaccaaga aggacctgaa atcgtacgtt atctaaaaga ggctggtcat aatgtatttt
    29221 tagatctgaa acttcacgat attccgaata cggtcgaaaa agcaatgcgg ggattggcaa
    29281 agcttggtgt tgatctcacc tgtgttcatg ccgctggcgg tattcgtatg atggaggcgg
    29341 caatgcgagg attagaagag gggactccag aaggtggaaa acgtccgcta cttctagcta
    29401 tcactcagct tacttctaca agtgaagaag aaatgcatgc cgatcaacta atcgaagttt
    29461 ctttagaaaa aagtgtgatc cattatgcta gctgtgccaa aaaagcagga ttagatggtg
    29521 tggtttcttc tgcttgggaa gtagaagcga tcaaagaaat ggcaggagac gaatttgttt
    29581 gcttaactcc gggtataaga ccagaaggaa cagtcgcagg agatcagaca agagtagtca
    29641 caccttctca ggcaaaaaag atcggctcaa cttttatcgt tataggtcga ccaatcactc
    29701 agtcaactaa tccttataaa gcatatcaaa cgattcaaac agaatggagc taacctaaaa
    29761 tgaatgttga acaaagtatc gcaaaagact tattagaaat tgaagcagta tttttaaatc
    29821 caagtaatcc ttttacatgg gcaagtggaa tcaaaagccc aatttactgt gataaccgaa
    29881 tcacgatgag ctatccaaaa gtccgaaaag agatcgcaaa aggattagca agcaaaatca
    29941 aagaagctta ccctgaagta caagtgattg ctggaactgc aacagcagga atccctcatg
    30001 ccgcatgggt agcggaaata ctggatttgc caatggtcta tattcgaagt aaggcgaaag
    30061 atcatgggaa aggtaaccaa atcgaaggac gtatatttga aggtcagaag atggttgtga
    30121 tcgaggattt gatttctaca ggcggaagtg tcttggaagc agctgaagca gcaaaaagag
    30181 aaggtgcgga tatccttggt gttgcggcta tctttacgta tgagttgcca aaaggaaaag
    30241 caaatttcga aaaagccgaa atccctctta tgacgttgac caattattct gtattgattg
    30301 aagcagcttt agaagatcgt tatatcgatg agcaagaatt aactctgcta aaagaatgga
    30361 aaaaagatcc tgaaaattgg cagatttaag cgcgcttgat gaagatagag aaatccgatt
    30421 tgtgtctcca gaatcatcaa ttggaatagc aaaaatccaa aaactgattg cgagaagcat
    30481 cttttcatca ggtatgaaaa gaaacttcta tcgtgatcag tttttgattt ttttaaaatt
    30541 catttttcta agactggtta gcagaaagaa gagaataatc tcttctcttt tttctattat
    30601 atatagattt tctctatttt ttggtaaggg agctgtttca aaaatggtaa agagcggaaa
    30661 tgttgcgaat atcagttatt taatgatatg atattcggaa aaagaaatct caaagggagg
    30721 taacttttat ggggttgaat gaatttattt taggattgaa caatttagag acgatcacaa
    30781 gggcacctgg attttttaaa tatacagagc atacagttgc agcacattcg tatcgtgtag
    30841 catctattgc gcaagttcta ggagacattg aagaagcgaa tggacaaccg atcgattgga
    30901 aactgctcta tgaaaaatca ttgaatcatg actacacaga gcgttttatt ggtgatatca
    30961 aaacgccggt gaaatacgca agcaaagagt taagagggat gctgcaaaca gttgaagaaa
    31021 agatgacgga cgaattcatc aaccaagaga ttccagctga gttccaagaa gtttatcgcc
    31081 gtcggttgtt cgaaggaaaa gatgaaaccg tagaaggaga actattagcc atttcagata
    31141 aggttgacct tctttatgaa tcttttgatg aaatcatcaa aaataacccg gaaaaagttt
    31201 ataaggagat gttcattgag gcagtgacta cgattaaaag ctttgaacat cggccttctg
    31261 ttcagtattt ctttaaagag atatttccgg aactattgaa ccaggaattt tatggaaaag
    31321 aggcgttttt agaaaccgtc tcaacgattg taaagaaaga agaaaaataa aaatgatatg
    31381 agatgcaggg ggaagtatag ttgaaaaaga atatggatgt cgaatggggc tattctggag
    31441 acaaaggtcc ggaaaattgg gcgagtttat gtgattggtt tgcgagaggt gcggaatttc
    31501 cattgcagtc gcctattcat ttgaccaagg gggaagaaac caaaatagaa ggagacacgc
    31561 tttcctttca ttatcaaaag caacggttta ctgacaagga attcaaaaac acgattcatt
    31621 tagtaccttt tgaccaactc agttatgtag agttcaagaa agaacgctat tatttgacag
    31681 atattcattt ccacttgcct tctgaacata tcatcaacgg aaaacaggaa gatattgaat
    31741 ttcattttgt tcatatgaac agcaaaaaag agaatctagt tgtgggtgtc atgtaccagc
    31801 taatggtaca ggaaggttgg ctgtataatc aagaaaatgg ggaaagttgg aacttagaaa
    31861 aacatgagca ttgggtaaat ccggatgtgt tctttccgga gcaaaaaagc catttccatt
    31921 atattggctc attgacaact ccgcctacca gcgggccaat ccaatggttt gtaatggatc
    31981 atgtaggaaa actgaaccaa gagtttcttc tccccttcga tggtcagtat gctcgaccga
    32041 acaaccgacc aatccagccc ttgaatggaa gagaaattta ttatttcgag gagtttgagc
    32101 aatgaatatt cagcagatga aatacgtggt cgctgtagca aacaatggta gtttcagaga
    32161 agcggcaaag aaaatgtttg tctcccagcc aagtctttca gctggtatca aagaattaga
    32221 aaccgagtta gggatcacgc tgtttactcg aacgaatcga ggggcatatt taacagaaga
    32281 agggcaagac ttcttaaatc gtgccgagaa aatattggtc cagcttgata cattagaaac
    32341 tcactatcta acgagtgaaa agagagacaa tttttcgatc gctgcccagc attatgattt
    32401 cttaggtcct ttgaccgcac agctactcga ggaattcaaa gacgagatca aacaatttcg
    32461 gatcatggag acgacaacag cgagagtgat cgaagaagtc aaagagcagc acagtgagat
    32521 cggcattatt tatatgaatg agcataatca agctggtatc agaagatatt tacaacaagg
    32581 agagctaagt ggccgcagca ttggaaaatt caaaacgcat atttttttaa ggcaaggaca
    32641 tccattagct gaacacgcag agatcaccaa agaagaattg ttatcttatc ctcaagttcg
    32701 ttttacacag gatggaaata atttcccgta tttttatgaa gaccttatcg agatccctga
    32761 tcaagaaaca gtgatttata cgagtgatcg aggtactttg atgaatatcg ttcttgaaac
    32821 tgatgcttac gcttcaggat ctggtattgt gattggaggg attcgcgagc acttgcggtt
    32881 aatccctttg gcagaaggcg aactaaatga attttatatc atttattcgg cgaaacgtcc
    32941 gctcagtgaa atcgctcaaa gatttatcaa agagcttact cggttactgg accagcagag
    33001 ctaagaaaag cagatgagaa cgctgtttat tgaacaccgt tgatcaaaaa agaggggttg
    33061 tgacagaatt ttgtcacaac tcctcttttt gtagcaaaca atttataact aattgctgtt
    33121 gtgggaaagt acgcaaatat gattctagaa acactcattg attacctgtt ttgtttcata
    33181 gctgtaattt tttcttcttc tggtgtcacg ataatcgtct tttggttttc ataaataaca
    33241 taaccaggct tggcgccatt tggttttcgg atatgcttta cctgtacaag atcaaccggc
    33301 acttgggcag agtatcggta ttttgaaaaa taggcagcta actcggcagc ttctgtaatt
    33361 gtttcatctg agggcttgtc acttttaatg atgacgtgcg aaccgggtat attttttgca
    33421 tgaagccaat agtcggtttt tttagctgtc ttcattgtca gctggtcatt ttgaagattg
    33481 tttttaccaa caagaatgag cgttccgtca gaagaaaagt attgatcagg ctgagatggt
    33541 ttcttacgtt tttgtttttt taagttcttc tttttcaggt atccttctga agtcaattct
    33601 tctttaattg cttcgatatc cattgggccg gcaatttcca gttgagataa gacagattca
    33661 agatattgga tttcatcttt ggcttcttgg atttgttcgc caatcagctt aacagcgttc
    33721 ttcagctttt ggtatcgatg gaaatatttc tgtgcatttt gatttggtgt caatgccgga
    33781 tctagtgcta ttttgattgg ccgatcttct tcataataat taggtaaaac aacttctttt
    33841 gcgccgcgtg gtacttgtgt cagaaaagta gtaagtagtt cgccatttct acggtaattc
    33901 tcagcgtttt cggattcttt gagtgtttgt tcacgttttt tcaatttatt tttatttcgt
    33961 tttagttcat tttcgatttt tcgaatcagt tctccgcctt gttgttttgc ccgatctttc
    34021 tctgcttttt cgtgataata gctatctaat aattgactga gtgttggata agctgttaca
    34081 gcagatgcct gttcggacaa tacctgataa gcaatcggtg tgaaaaaatc tttattttcc
    34141 gtttcgtaaa aagtaggaat aggctgatgg ctgatggctg agaaaaattc gtcccataca
    34201 accatctttt cattaggacg ttccagtaat cttttggtca gctcttgagc gatatcgaaa
    34261 ccgattccct gaaactgacg ttggattgct tttggatcta gttcagtttg ggaaagccgc
    34321 tgaaagattt tttctttctc ggagctaaat gggttcaaga cctcttgttt tggcggagca
    34381 acatattcta caccaggaag caaagaacga taagtgtttt gagagcttcc aatgtgtttg
    34441 atcgcatcca gtatttttcc agtttctcga ttaactaaga cgatcgtact atgacgtccc
    34501 attaattcaa cgatcaatat gatattttgt agatcaccca actcgtttcg tttagcaaag
    34561 tggaaatgga tcactcgatc attttcgatt tgttcgatgt cttctaaaat agcgccatcc
    34621 aaatattttc gaagcatcat cacgaagtta ggcggattat caggattctg ataatcgatt
    34681 tgggtaatct gtacacgagc ataacttggg tgcgcagata ataataatcg atggtttttg
    34741 ccgcgtgaac gaatcaccag cacgatttca ttttcataag gctgatggat tttcgagatc
    34801 cggccagata gtaacgtttc acgcaattcg ttgaccatcg cgtgggtaaa tacaccatca
    34861 aaggacatgg ttcattcctc atttcttttc ataagtatat tcattataac gaaaaaaaga
    34921 agaaaaacca atttggccga atagtaaagc aaaagtctag aaaaatcttg gactaattac
    34981 ataaaaaaca gtgtttctat cgttgaaatc caaattttta ggtataataa aaaataggag
    35041 aaaaagtaga aaagaaggtg tttgttattt gggaagtagt taaatcgaga gtacaagaaa
    35101 tgaatgaaca tttctcagtg tcattcgcag aaaatccaag taagtatttt cgacgtcttg
    35161 tttttctgct catcttatca ttgctgttaa cagttgcaat gtttttattg ccggaaaaaa
    35221 tccgagaaga gcaagtaaat agtctcgttt attcgtccat accaaccagc aaattgaaac
    35281 cttttgatta cagcaaagct gatcagaaaa tcaaacaaag ccgggcgata tcggtgatgt
    35341 tcagcaaacc gaacgggaaa acctatcagg acatgcttga tgtttttaat gatcctgagc
    35401 ttatggaaga attgaatcga ccgatctttt attatccgat cgtttatgat gtacatgaac
    35461 tggaacaaaa atataacata cgaacggatg aagtgacatt tattttcttt gatggaggaa
    35521 aagaagcaaa tcggataact gcgggtaagg gaggaataac tgattttgat aaggagttga
    35581 tccctgaact gaatcgactt ccgttagcca atatcaaaca attagaagaa gaattggata
    35641 gaacgggagt aacttcccaa aattccgaga ttgctaattg agacattggc gcgatgaaac
    35701 ggtgggtcta tatagcgcta tttttaatta tcagaacaag tgatgtttga tcttcaaaat
    35761 aacaaataaa aaattaaaaa atatagttga cgaaattcaa aaattcgatt tgacttaaat
    35821 caattcttaa ggaaagggtg acctgtaatg aacttatcaa aacgtatttc agcagaaaac
    35881 aaaaaaaatt ggcgcagctg gcgtagataa gttgtattaa tggtgagact ttcccataga
    35941 tacaactttt tagagtgcat ttctaaagct tgtatctatg ctagacatga acattgactg
    36001 ccgcatagat tagaatctat gcggtttttt gtgtaaagac aatgaccgct ttggccattg
    36061 tctttttttg tagactggat acttgcctga ttataataaa taaaaagatt gactttgcag
    36121 gaggaaaaaa atgaaaaaca aacgattaat aacagttgta gtaattattt tagtttattt
    36181 agcaggtgca tttatttatg gaaaagcctc aggcgaaaaa gaaagtactt cgacgaacca
    36241 gacaaaaaaa acaacagttg ttggggtatt gcaatacgtg agccatccgg cattagatga
    36301 gatttatcga ggaatcaaag atggtctgaa agaagaagga ttaaaagaag gagaaaatct
    36361 tgagatcaaa tttcaaaatg gacaagccga tcagagcaag ctagccacaa tgagtcaaca
    36421 gctagtacaa tctgatccag atgccttgat tggaatcgca acacctgccg ctcaatcatt
    36481 agcaaacacg acttcatcga tcccagttgt gttaggtgct gtaacggatc ctgtgggggc
    36541 tggattagtc gcttctttag agaaacctgg tggtaatgta acaggtgttt ctgatcagcc
    36601 gcctgttact tcacaaatca agcttgcttc tgatttgttg ccaaatgcaa aaaaagtcgg
    36661 aatcttgtat gcttcttcag aggataattc aaaataccaa gtagcacaag cagaagaagc
    36721 tgcaaaaaaa gaagggttag aatcagtaaa atatgcggtg ccttccacta atgagattgc
    36781 acaaacggtt caagtgatga gccgacaagt cgattttatc tatgttccat tagataatac
    36841 cattgccaat gcgatgcaaa cagtggtaaa agaagcaaac aaagcaaata tccccgtcat
    36901 tccatctgtg gatacaatgg tggaacaagg tggactagca acaatcggga tcaatcaata
    36961 tcaattaggg cttcaaagcg gtaagatggc ggcagaactt gcgagtggaa aagaaaaacc
    37021 ggaaacgacg cctgtttata catttgatca aggggataca gtgatcaatc aatctcaagc
    37081 tgatcgctta ggaatcattg ttccccaatc gatgaaggaa aaagccaaaa tcattacaga
    37141 tgactcgcag caagaaacat ctaaggagga tgacaaatga tcgtatcagc tatttcacaa
    37201 ggcatgcttt gggcaatatt aggattaggg attttcatga cttatcggat cttagacttt
    37261 cctgatatga cgacagaagg gtccttccca ttaggtgctg ctgtctgtgt gacagctatt
    37321 actcatgggg tagcgccgat tgtagctaca ttattaggcg taggtgctgg catgatagca
    37381 ggactcgtta cggggttact ttatacaaaa ggaaagatac cggttatttt agcagggatt
    37441 ttagtgatgt caggattgaa ctcagttatt ttgtttgtga tgcgaacacc aaatctttct
    37501 ttattgaatc atccgattct acaagacagc ttccatcgct ttgggttacc tgattacttt
    37561 gacacagtcc ttcttggctt catctctttg ggcatcttga ttgctgtact gctattcttc
    37621 ttcaatacag atttgggtca aggctatatt gcgacaggag acaacgaaac gatggctcgg
    37681 tcgttaggga taaaaaccga tcgtatgaaa attttaggtt taactttgtc gaacggtgtc
    37741 attgcattat ccggtgcgct gattgcccag aacgacggat atgcagatgt aaataaaggg
    37801 attggcgtaa tcgtcatcgg tcttgcttcc atcatcatcg gtgaagtgat cttccaagaa
    37861 ttgacgctag cagaaagatt gattgctatt gttgtcggca gtatcattta tcagctattg
    37921 attttagtag taatcaaact agggtttgac acgacttatc tgaagttgtt ctccgctatc
    37981 atcttagcta tttgtctgat gatcccgcaa ttgaagaaag cactgaactt aaaaacgggt
    38041 tttgagaagg aggcatagta atgactgata tattgacaat caaaaatggc agaaaagtcg
    38101 taaacaatgg aatgaatgaa gagaaagtac tattggatga aattaacctt acaatcagag
    38161 aaggggactt catcactgtc cttggcggga atggtgctgg aaaaagcaca ctgttcaata
    38221 cgatcgctgg tacgcttcaa ctcagtcaag gatcgatctc ttttaaagat cagctgatca
    38281 caaaagattc agaagaaaaa agagcacgat tcctttctcg agttttccaa gaccccaaga
    38341 tgggaacggc tcctcggatg acagtagcag aaaatttgct acttgcacaa aaaagaggtc
    38401 aaaaacggaa attccgttta cgtaacttga aaaatcaaaa agaagatttt ttcagactat
    38461 gtcaagaagt cggtaatggc ctagagcaac acttggatac tccagcgggt gaattatctg
    38521 gtggtcaacg ccaagcatta agcttgctga tggcgacgat cacagctcct gagttactat
    38581 tattagatga acatactgca gctttagatc ctaaaacagc taaagcatta atgcgattaa
    38641 caaatcagcg gatcaatgaa caaaaattga cttgtctcat gatcacgcat cgaatggaag
    38701 acgccttgaa atacggaaat cgtctgatcg tactccaaaa agggaaaatc gttcaagata
    38761 tttctgaaaa agaaaagcaa catttgacaa tgggcgacct tttgcagttc tttgaagaag
    38821 tagtggattg atttcgacag aaaaattcct ttcagtagaa ggacttgaaa actcattgac
    38881 atcttccttt gattcatcct atacttaaaa caataaacaa ttaaacatat ttgggtgttt
    38941 tgtaggatca cgtagtcaat gattttaaca attaaataga atattctttg ctctgacaag
    39001 taaacgatga gaaaagaatt tgctactttg ccacctagac gtttcgtact ttttcgtatc
    39061 aaacgctaag tggctttttt tattgaagta agaacaaagg aggaataaga tgatttctgg
    39121 acaaaccaaa ttagctgggc tctttgctga tccggcttct cacagtttat ccccattgat
    39181 gcacaatact gcttttgaag caaacaacat cgatgcggtg tatttagctt tccaagtaaa
    39241 tccaatgaat ttgaagcaag cagtcgaaag tattcgaact tttgatatgt taggtgtaaa
    39301 tctttcgatg ccgaataaaa cagcggttat tccttatctg gatgaggtaa gccaggaagc
    39361 gcaattgatc ggagcagtga atacgatcgt gcacgaaaag ggtcgtttga ttggctataa
    39421 tactgatggt gcgggcttca tgcaatccgt gcaagaagct gggatttcga tcaaaaagaa
    39481 aaaaatgact ctactgggag ctggaggtgc agcaaaggca gtcactgttc aagcagcgtt
    39541 agacggcgct gaggaaatcg tagtttataa acgaaacaac caaacattcg ctcaagtaaa
    39601 agaaacattc gaacaactca gctatcgaac ttcttgtaag ataaaagttc gtgactttgc
    39661 cgatacgcat caattaagaa aagacttgaa agaaagctgt cttctagtca atgcaacaga
    39721 tatcgggatg ggaaagaaaa tcaatcagtc accgctctta gatataagca tgatgcatga
    39781 gaatctagcg gtttttgatt tgatttatgc acctagagaa acacgtttat taaaagaagc
    39841 aaaaaaagta ggagcaaaag gatacaatgg acttggcatg ttgatctacc aaggagcaat
    39901 cgcgtttgaa ttatggacaa aggaaaagat gccgatccat gtgattgaaa agctgtttga
    39961 ttaaaaaagg ataatagata aagatctttt attctcgcat aaacggtctc aagaaaagta
    40021 atcctttatg tatcgaagca tttatttcgt tattttttat taaattatca aaaaatgata
    40081 ggcttttgtc gattggttat cgtatccttt atttatcagt aagaaacgag gaggaaccac
    40141 aatgagtcgg atggatcgct ataaggacat ccataagaag gcgaaacctc tgaagcggga
    40201 tacggggagt atttttgcta gaagagaacg aaaacgaacg cgtatggaaa atgaggaaga
    40261 tgctacccgt atttatgaaa gagagcagca ttcatttgaa catttgaacc aatacgagca
    40321 acagatatac agagatgcac caagagcaga agaacaacaa aaaaataaaa aatttttcaa
    40381 tcggcaaaag aagaacaaac cgttaaaacc tcgaagaaag cgatcgtgga aaaaaatcat
    40441 tctatcgatt ttgcttgttt tgatccttta ttcaggtatc tcatttttta ttggtaaata
    40501 tgtagctgaa catgacacaa gcttagaggc agcgacttca gaaacctttc atgggcaaaa
    40561 agcgacgaat ggtgcgcata atattttgat ccttggaagt gatacacgcg gagaagatgc
    40621 cgggcgagcc gatacgatca tggtcttaca gttggatggt ccttcccata agccgaaact
    40681 gatttctttt atgcgtgaca gcttcgttac gatcccaggg gtaggacaaa ataagatcaa
    40741 ttcagcttat gcatacggag gagccgatct agtgcgtcaa actttagtag aaaattttgg
    40801 aatcgattgt caatactatg cgaaagtcga tttcaaatcg tttgaaaaag tgatcgatgc
    40861 cttattcatg aacggagtga agatcgatgc agaaaaagat ctgaatctgg atggtgtaga
    40921 tattaaaaaa gggggccaga agatggatgg acatgttctt ttgcagtacg cacgtttccg
    40981 aatggatgaa caaggagact ttggccgtgt cagacgccaa cagcaagtaa tgaacgctat
    41041 tttcagccaa ttgaagaatc cattgaatct gatccgtgca ccttatgcag ctggtaaagc
    41101 tttaggatat acatccacag atgtcactgc ttggttcttg acgaaaaacc tcttttcgat
    41161 tgcacgagga gctggtggca ttgatcgaat aagtgtgcca gtagacggtt cgtggaatta
    41221 tgggcaaagt gattatgctg gcagtattct tgaaattgat aatcaaatga atcaatctgc
    41281 tattacagat tttttgaata aatgatcata aaaaagatca agaagaagct gcgcaaggct
    41341 tttgtcatga atcaatcaaa cgattcactc gtaaaaaacg ctttcttatg gtagatttaa
    41401 ccaagagagg agctgtttac attggatatc tatctgaact tcaaaaatga gtcgcgtgaa
    41461 gccatcgctt tttatgaaaa agtttttcag actgaagcat caggtatcac cacatacggt
    41521 gaaatgcctg gaatagaaca aatgaacttg ccagaagaag caaaaaatct aatcacgaat
    41581 gcacggctga acatttatga tacattagtt atgtttgcag atacgccaga aggaattggt
    41641 cctgaattag ttgtgggaaa taatgtttct ttagtcatcc agacaagaaa tgaagaagaa
    41701 acgaagcgcc tatttgaaag cttagctgag ggtggtaaga ttgagatgcc actggatgct
    41761 gttccgtggg ctcagttgta tgggatggta actgatcgat ttggcatcaa ctggcagatt
    41821 aaccaagagt gacagctaaa gatgaatatt ttctggaaac atcaagcagc aaacggatca
    41881 tcgtttgttt gcttgatgtt tttgtgtcta tttcgactat acggtatctt ttttttattg
    41941 ccagatgcga aaatatgttc cataatagag aagtccgcaa aaaattaagt aacggaggtc
    42001 agaagatgaa gacggtgata ctagctgaaa aaccttcaca agcaaaagcc tatgccgaaa
    42061 ccttccaaca gcaaaagcgg catgaagggt attatgaaat cagcgatcca ttgtttgctg
    42121 gtgaagtaac catcacttat ggttttggac atttggtaga tatggttgct ccaggagctt
    42181 atgaagagcg ttgggcaaaa tggtctttag aaaatttgcc tattttccca gaaaccttcc
    42241 gttacgaagt tccaatggac aaacgtgcac aattttcgat cgtaaaacga gaattgcagt
    42301 cagccgatac catcattatt gcaacagatg gtgatcgaga aggtgaagcc atcgcgtggt
    42361 cgattattcg acaagcaaac gcattttcta aggaaaagaa ctatttacgt ttatggatca
    42421 attctcttga aaaagaagca atctatgaag gttttaagaa tctccaacct ggaaagaact
    42481 attttccaaa atataaagaa gcgcaagcaa ggcagaacgc tgattggctg attggaatga
    42541 atggaagtcc attatatagc ttgctacttc aacgaaaagg gattgatggc agtttttctc
    42601 ttggtcgtgt acaaacaccg acactctata tgatttatca gctgcaagaa gaaatcaaac
    42661 acttcaaaaa agaaacgtac tttgaaggag aaggtttaat tacgacaaac aaagggcagt
    42721 tttctggaaa aatcgtacca aaaaaagcat ttaaaaccca agaagagtta acgaaggaaa
    42781 tagaaaaact tggagcgcat ttaggtaagc aaaacggacg gattcttgaa gttacgaaaa
    42841 aggaaaagag gttgcatagt ccacgtttat tttcgctatc tagtttgcag acaaagatga
    42901 atcagctaat gaaagcaagt gccaaagcta cacttgatgc tgctcaagga ttgtacgaaa
    42961 gcaagtttct aagctatcca agaacggatt ccttttacat tacagaaaat gagcatcagt
    43021 atctggttaa acatctgcaa aattataaaa aatttttagg aatagaagaa gtacagacaa
    43081 ctgagatcaa agcaaagaaa cgatatgtcg atgcgaaaaa agttcaagaa catcatgcaa
    43141 ttattccgac aaagacgatt ccgactcctg cacgctttgc gaagctttcc aaattacagc
    43201 aagcaatcta tttgcaagtt ttgcgcacta ctgtggctat gtttgctgca gattatatct
    43261 acgaagagac gaccgtaatg acgggcgtac agcaattaaa gctaaaaagc atcggaaaga
    43321 tcccattaca gcaaggttgg caggaaatct tacgcatcaa gtcaaaaaag aaagaagctc
    43381 agactcttcc tcctatcgta gaaaatgaag aagcagaagt agaactgaaa agtatggaga
    43441 aagaaacaca acctccaaaa ccctataccg aaggaacgtt gatcacagca atgaaaactg
    43501 cgggaaaaac gctggataat gatgaagcgc aagaaattct caaagaagta gaaggaatag
    43561 ggacagaagc tacacgagcc aatatcattg aaaatcttaa gcagaagcat tacatcgaag
    43621 tcaataaaaa cgaaataact gttacgccaa aaggaatcac tctttgtaag gcagtagcca
    43681 atgaaccatt actgacgagc gcagaaatga ctgcgcgctg ggaagggtac ttgagaaaaa
    43741 ttggtaaaca agaggggaca ccatctgttt ttttagagaa tatcaaaaaa ttcatcctac
    43801 atttattggc agaagttcct aatcaaatcg acaaggtaga cttttcagat gagattagcg
    43861 ggacacgtca gataaaagcg atggagaaga aaaacgatca attaggaaga tgtccaaaat
    43921 gtaaaaaggg aatagtcatg ctttatccta aaatcgctac atgcttgaat ccggaatgcg
    43981 acttcaaatt gtggccaaca gtagctaaaa agaagctgac taaaacgaat ctcagagatt
    44041 tgctttcaaa aggaaagaca agtaaagttg tcaaaggttt tactggtaaa aaagggaaat
    44101 tcgatgcagt tttagtatta aaagaagata tgacaatcgg tttttctttt ccatggatcg
    44161 aaaacagtgg aacaaaagca gttgatggaa acaaagaaac atcaagaaaa aataatgaaa
    44221 acgaagtaga gtaaatgaaa acaatgaaat agcggaatat ttattccgaa aagtgagaat
    44281 ataaatacca ataaaactaa aaaatatcag tgaatcaagc aaaaacgttg gggtaggctg
    44341 attttatata gatagcaagg agttttttta tttgaaaaaa gtgcattcat ttttaaatta
    44401 tcttatggga gcaatcacga ttgtggctgt gtaccaagca tttctaaagt atcctgatta
    44461 cgggacgaag ttgttgtcat tgacgcacaa tcaaacagca tattcaccaa cgttgatttt
    44521 atcgactttg actgcaggag ctaaaagcgc tacttatttt tttcataaaa cgacaagcat
    44581 gctaaagcga aagaaacagc gaaaacaatt gttggtggtg gtagcggcta tgatcattat
    44641 ttattttcta ccagttatta cacaagtatt tcatcaatat tttggttaag aatacgagag
    44701 tttgtgacaa aagtcatgtc acaaactttt ttctttgtca ttttttcgta agaaaaagca
    44761 aaataaaagt atatacattg attaaaagta tatacttgga tataatgtac ttactataga
    44821 atgagaatgg tatagagggt ggcgaaatat gaaaaaaatg aaaaagtata aaattagaaa
    44881 atctggcaat agcgatgtaa caactatccc accagaagta aaagaattca tgggtgtgca
    44941 gacaggtgac gcaatcagtt atgtttttca atctgatggt tctgtacgaa tgatcaaagc
    45001 acaagaagaa cctgatattg attcattagt tgattcgatc atgaatcaat atgaagacgc
    45061 gttaaaagat ttggttgatt tatgaggtat ctttcgacta aagaaattat caaaatcaac
    45121 gcgaaagtta ttttacgtca ctcgccaggc gaaatgatcg gtgtaaaaga tgcaaatgca
    45181 ttagatatgg ccgtcaaaca gccttcacaa gcagtattca atcaagaact ctatcctgaa
    45241 atctatgaaa aagcggcgat tttagcaatt aatttagcta aaaaacatcc ttttcataat
    45301 ggcaataaac gaactgctct tgttgccatg ttgttgtttc ttcaattgaa taattgtcca
    45361 gttgcttttt ctcgtcaaga agctgttgat tttatcataa tgattaccac tagttcattg
    45421 gatttcgatt cactaaagtc caagataacc agttatttac gacaaaccgc aataaaatga
    45481 acgaattaaa aactttattt ctcaattaga tcagcatgtc attaaggaat atgctgattt
    45541 ttttatgttt aatcatacaa aatagttttt tcataaaaat atggaaaata tggattaatt
    45601 ttgaaataat tgatttaaaa ggaatacaaa agagtgaata gtaggaaata gtggtttaga
    45661 actagtgggc tgatttttta tatattttag tttacataat atatattata caaagtagat
    45721 ttaaaaaatg ataaacctct ctcgaacggt tttttatcaa aaatcgtcat ttttccatca
    45781 aaatctgctt atgcaaaatt ataaaaaaat tttgcagttt ttcttacttt ttttgtcgtt
    45841 taattgaatg ataaaaacca gtttctttat tcagttaatc aaatatgaac gaattagata
    45901 tgaatcattg tatttgccgt tgcggaagga ttttttaatt aaccacagat aaacattgat
    45961 aacgattcgc ttattgtaat caacagatta aatttcatat gttcatatgt acggatacat
    46021 tttcggaccg attaatttta accattctgt ttatttgctt acctattaga tatcaataaa
    46081 aaactgctta acagctattt cagctgttaa gcagttttag gatttttagt tcatattcat
    46141 tgtttcttat aatacagtgt tcaatacttc tcttaaagca cttgttgatt tgctgaacat
    46201 tgctttttct tcttcgttga ttgataattc aacaacttca cgaacaccat tttcgtcaac
    46261 gattgatggc acacctgtaa agatatcttt ttcgtcgtat tcaccagtta aataagcaga
    46321 tacaggcaat acagcttgtt cgttattcaa gatagctttt acgatacgtg cagtactcat
    46381 accaatacca tagtaagttg cttttttacg atcaatgatc tcataagcag cattacggac
    46441 tttatcagca atcacatcta attcatcttg ggcaatacga tggtcttttt ctacaatttc
    46501 aaacactggt ttaccaccga cagttgtatg tgaccatgca gcaacttctg aatcaccatg
    46561 ttcacctaag atatagccgt ggacgctgcg tgggtcaact tttaatttca atgcgatttc
    46621 tttacggaaa cgagttgtat ccaatgttgt tccagtaccg ataacacgtg atgttggcag
    46681 accagattcg ttccaagcga tatatgtcaa aatatctact gggtttgaag caacaacgat
    46741 aataccgtcg aatcctgatc ccatgatttc ttttacgatt tggcgcatga ttgacgcatt
    46801 tgttttaact aaatctaaac gagattgacc aggtttttgg ttgataccag ctgtgataac
    46861 aacgatatca gcatctttac attcttcata gccacctgac catacagcca cattttcatc
    46921 gccccaagcc ataccatcta aaaggtccaa tgcttcccct tctgcttttt cttgattcac
    46981 atcgatcaat actaattcat tagagattcc ttggttgatc attgcataag cgatacttgt
    47041 tccaacaaaa cctgtaccga cgattactac tttacgactt gtttttttca tgaataaaca
    47101 gctccttttc ataactattt gttactttca tgaaaagtat agtcttgttt attcatttta
    47161 tcaacatgtt aattataata gttgttatta taatttttga tggaaacgaa ttcctgaata
    47221 cgcataacag ataaatgaca aatgtttaca cctatatccc ctgataatac aaccaaaagt
    47281 ttagaactga aaataaatcc aaaaaaattc gctgcaacga tataataaga cagcaatcgc
    47341 acctaaaaca attttccttc atatgcttta gtgaaagatt ttcttcttaa tgtttatttc
    47401 tccattcatt gggagtacat gttgaatact tcttgaataa tttgaataaa gaagtcgttt
    47461 gtttatatcc gatacgatta gcgatttctt ccaacgaaaa actcgtttct tgaatcagtt
    47521 ccaacgcaaa
//
LOCUS       DACEOE010000007        39097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEOE010000007 DACEOE010000000
VERSION     DACEOE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.007"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2013
                     /locus_tag="IW774_000338"
     CDS             <1..2013
                     /locus_tag="IW774_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1152718.1"
                     /translation="ESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNSE
                     SQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDYE
                     FYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVYV
                     DALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIGE
                     NINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYL
                     ALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHL
                     AEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGL
                     LFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQ
                     RKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIE
                     FLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWF
                     AIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSAS
                     LKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2010..3803
                     /locus_tag="IW774_000339"
     CDS             2010..3803
                     /locus_tag="IW774_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1152719.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            4003..4317
                     /locus_tag="IW774_000340"
     CDS             4003..4317
                     /locus_tag="IW774_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1152720.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            4338..4715
                     /locus_tag="IW774_000341"
     CDS             4338..4715
                     /locus_tag="IW774_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1152721.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            4725..5498
                     /locus_tag="IW774_000342"
     CDS             4725..5498
                     /locus_tag="IW774_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152722.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            5520..6923
                     /locus_tag="IW774_000343"
     CDS             5520..6923
                     /locus_tag="IW774_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1152723.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            7105..8289
                     /locus_tag="IW774_000344"
     CDS             7105..8289
                     /locus_tag="IW774_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1152724.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            8286..8576
                     /locus_tag="IW774_000345"
     CDS             8286..8576
                     /locus_tag="IW774_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152725.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            8573..8794
                     /locus_tag="IW774_000346"
     CDS             8573..8794
                     /locus_tag="IW774_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152726.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            8836..9615
                     /locus_tag="IW774_000347"
     CDS             8836..9615
                     /locus_tag="IW774_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ1152727.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            9682..10176
                     /locus_tag="IW774_000348"
     CDS             9682..10176
                     /locus_tag="IW774_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1152728.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            10232..10870
                     /locus_tag="IW774_000349"
     CDS             10232..10870
                     /locus_tag="IW774_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152729.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            10942..11337
                     /locus_tag="IW774_000350"
     CDS             10942..11337
                     /locus_tag="IW774_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1152730.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            11321..13774
                     /locus_tag="IW774_000351"
     CDS             11321..13774
                     /locus_tag="IW774_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1152731.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            13771..15798
                     /locus_tag="IW774_000352"
     CDS             13771..15798
                     /locus_tag="IW774_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152732.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            15795..16817
                     /locus_tag="IW774_000353"
     CDS             15795..16817
                     /locus_tag="IW774_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1152733.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            16834..17763
                     /locus_tag="IW774_000354"
     CDS             16834..17763
                     /locus_tag="IW774_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1152734.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            18008..18124
                     /locus_tag="IW774_000355"
     CDS             18008..18124
                     /locus_tag="IW774_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1152735.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            18140..20059
                     /gene="tet(M)"
                     /locus_tag="IW774_000356"
     CDS             18140..20059
                     /gene="tet(M)"
                     /locus_tag="IW774_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1152736.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            20157..20345
                     /locus_tag="IW774_000357"
     CDS             20157..20345
                     /locus_tag="IW774_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1152737.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20403..20756)
                     /locus_tag="IW774_000358"
     CDS             complement(20403..20756)
                     /locus_tag="IW774_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1152738.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21287..21709
                     /locus_tag="IW774_000359"
     CDS             21287..21709
                     /locus_tag="IW774_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1152739.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21706..21936
                     /locus_tag="IW774_000360"
     CDS             21706..21936
                     /locus_tag="IW774_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1152740.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22434..22634
                     /locus_tag="IW774_000361"
     CDS             22434..22634
                     /locus_tag="IW774_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1152741.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22661..23854
                     /locus_tag="IW774_000362"
     CDS             22661..23854
                     /locus_tag="IW774_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1152742.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23922..25487)
                     /gene="guaA"
                     /locus_tag="IW774_000363"
     CDS             complement(23922..25487)
                     /gene="guaA"
                     /locus_tag="IW774_000363"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1152743.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25546..26469)
                     /locus_tag="IW774_000364"
     CDS             complement(25546..26469)
                     /locus_tag="IW774_000364"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1152744.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26665..27270
                     /locus_tag="IW774_000365"
     CDS             26665..27270
                     /locus_tag="IW774_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1152745.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27578..28594
                     /locus_tag="IW774_000366"
     CDS             27578..28594
                     /locus_tag="IW774_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1152746.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28735..30036
                     /locus_tag="IW774_000367"
     CDS             28735..30036
                     /locus_tag="IW774_000367"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1152747.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30082..30744
                     /gene="deoC"
                     /locus_tag="IW774_000368"
     CDS             30082..30744
                     /gene="deoC"
                     /locus_tag="IW774_000368"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1152748.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30765..31163
                     /locus_tag="IW774_000369"
     CDS             30765..31163
                     /locus_tag="IW774_000369"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1152749.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31229..32311
                     /locus_tag="IW774_000370"
     CDS             31229..32311
                     /locus_tag="IW774_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1152750.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32523..34085
                     /locus_tag="IW774_000371"
     CDS             32523..34085
                     /locus_tag="IW774_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1152751.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34078..35211
                     /locus_tag="IW774_000372"
     CDS             34078..35211
                     /locus_tag="IW774_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1152752.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35211..36167
                     /locus_tag="IW774_000373"
     CDS             35211..36167
                     /locus_tag="IW774_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1152753.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36266..37432
                     /gene="deoB"
                     /locus_tag="IW774_000374"
     CDS             36266..37432
                     /gene="deoB"
                     /locus_tag="IW774_000374"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1152754.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37456..38274
                     /locus_tag="IW774_000375"
     CDS             37456..38274
                     /locus_tag="IW774_000375"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1152755.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38291..38998
                     /gene="deoD"
                     /locus_tag="IW774_000376"
     CDS             38291..38998
                     /gene="deoD"
                     /locus_tag="IW774_000376"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1152756.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        13031 a         6653 c         7984 g        11429 t
ORIGIN      
        1 gaatcagatt tttttcatgg aattcaaaat gggtggcaag ggcattggat tattatctct
       61 gcgacatttg aaggaatcag cgaagaagaa tttgacaatg agatttgtgc ttccatttct
      121 ttaaattctg aaagtcaaac aatcttagaa gaattgattt caaatgcaga taaaggtgta
      181 ggtagtattt ctctatttat tagacctaac aaagctatta gaaagcaatt atttgatatt
      241 tccactgaat tagactctca tcaatttaac gaatttagaa attctattcg tctatccgac
      301 tatgaatttt actacacatc taaatcaaat ctaaatttct gtatcaatga aaattatgat
      361 aaattggtag gaagactcaa tgacaatata gctagtaatc ctgaagaaga cgatgaagct
      421 ctgttgggct caagaattga catgtcagat attttcaagc atatttcggt tgtttatgtt
      481 gacgctcttc gagatgttct tagagaaatg aaaaacaatc gaaacccagt caaaagaatc
      541 atggaaacaa ttgaatcaaa aatatcatca gataatgttg agtcattaaa aacaattata
      601 cagcaattaa atgagactat taccagtgtt cctgaaatta ggaaaattgg cgaaaatatt
      661 aatcggcaac taaatagcat aattggttca gtgtattctc ctaatcttct tctttcctca
      721 actatgtcag atgatatggg ttctttagca aaatttcttt caatgaagcc agaacaaaat
      781 atcgacttag acttactagg gttaggacat ctaaatatga tttatttagc tttgaaaata
      841 gtcgaatttg aagcgtgtcg ttcaagagaa ctacttaata ttatgttgat tgaagaacca
      901 gaagctcata ttcatcatca tattcaaaaa actctgttcg aaggattaaa tcttcaaaaa
      961 aactatactc agattctcat gaccacacat tctgtacatt tagctgaagt gtctgaaata
     1021 tctcggatga atgtattaaa atcgtttaat ggcaaatcta tcgttatgaa tccagcaaat
     1081 aatctgaact catttgctaa agaaaagtta aaaaagaaca atctaaatct aataaacgca
     1141 gtagagagat atttagatgt taaaagaaat ggattgcttt tttctaaagg tgttatctta
     1201 gttgaaggag atgctgaaga aatacttatt cctcaaatag taaaaaaatt atttggtata
     1261 aacttagacg aacttggaat aggtttgatt aatgttggta gtacttcgtt tgaatatatc
     1321 gctagcttgt ttgacgatga tagaatacag agaaagtgtg ctattgtaac tgatttagat
     1381 gaacaggcaa tcccatcaga acatcgattg tataaaagca ctgcacaaga gaaaggtcaa
     1441 gcaagaaaag aaaaattatc ctcccttttt aatgaaaaca attgggttaa ttgtttttat
     1501 gctaatacaa catttgaaat tgagttttta gatgtgaata aagaaaatat tctaacttat
     1561 gtcagtccaa ttatagatga cgcattcaaa gataaaaaaa caataactgc ttataaagaa
     1621 gaaatacaaa gtgaacaata tttagaaaga aatttagcga tgttaaaact agccaatcat
     1681 gttggtaagg ggtggtttgc aattgagtta ggtgaaaaaa ttgacttttt aatcaaaatt
     1741 cctaagtata ttcttaatgc tattgctttt gcatgtcagg aaatagtaga tctctcaatt
     1801 tataaaaaaa ttctactcca tacaatcgac ttgtacgagg aaactacaga attgtcagct
     1861 ttatcagcta gtttaaaaac aagtgataca gctgatttga atatagcaat aatttcttta
     1921 ttagagtact ttggtaaaga tgaggatgtt gtgtatctaa tcaatcagat agaaaattac
     1981 acaaaaatac tttatgctga ggtttcaaaa tgacaaatat cttcgaaaaa atcgtaaatg
     2041 atttaaattt agaacaaaaa gatgttgtgg aaagtgaaga taacctttat gtaaatgcat
     2101 gtcctgggag tgggaaaaca agggtactta caaggaaaat tgcttaccaa gcaattaaac
     2161 acgatggatc actaaaaaga ataattgcta tcacatacac taatagagct gctgaagaaa
     2221 ttaaggaacg cctaagttta cttagtatag atgatgatat taatatttgg gtggggacaa
     2281 tacatcagtt ttgtcttgaa tttattattt atccttttaa aatgaatctc tcaagagtat
     2341 caaaaggatt tacaattatt gatgattata ctcaaagagc ttacgtcagc caaattaata
     2401 atatgctgaa tttaaatctc aaaacgtatg aactgaacaa aattgatttg tcactttctc
     2461 atgattttaa aattaatgaa gttcagtata ctcaagtttc tgaaatgtat cacactatgt
     2521 tggaaaaaaa taaagaaatt gattttgata tgattttact agtagcgtat aaaattttaa
     2581 ctgtaaacag taacgttgca cgtaatattg ctagtaatat aagggcgatt tatgtggatg
     2641 aatttcaaga tactagagaa ttacaataca atgtaatagc taaattactt caaaacaacc
     2701 ctcaaattca gtcaatgttt gttggagata ttgaccaagc aatttatgga tcattagatg
     2761 gtatagctaa atcagttgaa gaactagaaa tgctaactag tcacactttt caatcaaaga
     2821 cgctacatgg ctgttacagg tctaatcaga gattagtaga cttctactct aattttcaga
     2881 gttgtcctta cgagattgaa agcagaggga ataataaaaa cgaccgtggt tttataagtt
     2941 atgattgcaa agttactaag gaggatttac ccaacataat aaaaaatata ataaaggaaa
     3001 agattgaaag cggcattcat gaaaaagata tttgtgtact tgctcctcaa cactatcctc
     3061 tgttttcact gggtgagtat ttaaagaaag aattaccgta ttgcaatttt gatgctataa
     3121 atatatcacc aattaaggta aataatcata gtttattttt caagttagca attttgtact
     3181 ttactgaatc aggtgaaaag gttcgaagaa gaaagctaat tgcaaacgat attttaatta
     3241 tattaaaaaa tgaatttgga atagaagtta aagattattt tattgactta gatttattac
     3301 agctaataaa cagtgtgaaa agaatgtata gtggagacga caacggtgtt gatttataca
     3361 aatttgttac caaacatata tttgttaatc ttaatattga cgagaaaaat tacccgtcta
     3421 tttttaagca ctatctattt tttattaacg aagtagaaga tcggataaaa aataattcat
     3481 tatctagtag tgtatctagt tttaagaaga tgtttaaatc tcgagatggt atcacactta
     3541 ctacagcaca taaagttaaa ggaaatgagt atgatactgt tatcgctatt aatcttctaa
     3601 acgggaaaat tcctcattgg aatgaaattt ttaatgtttc tgataaagga attagttcag
     3661 cgagaaaact actatacgta atttgttcaa gagcaaaaaa taatttattt cttttctcag
     3721 aggtgggtta ttttacgagt aaaggagact tgctttatcc cactcaatta ttagataaaa
     3781 tagaatttga ttataatatt taaaatacct aggtagtcgt tgttttaaat atagtaggaa
     3841 tcggacgctc agtacggagc gtctattttt ttacccaatt ttgaaaggaa gtgaacttat
     3901 gaaaataaaa aatcaagaat caaaaggtcg ctctcccccc tttaaaacca tcacacaaat
     3961 aagattcatt caaacataaa aacagaaagg ataggtaaaa atatggaact taaatatgtc
     4021 attcccaata tggaaaaaac atttggacaa ttagaatatg ctggtgaagg gaacattgaa
     4081 caacgtcgaa taaatggcag accaaccatt ctctctcgta gttacaattt atactcaacg
     4141 attcaacgag cagatgatat tgttgtcatt cttccctccg aagctggcga gaaacatttt
     4201 gaatccgacg aaaaagtcaa actcatcaac ccacatatta ccgcagaagg ttataaaatt
     4261 ggcaatcgag gatttaccaa ttacattatg cacgcagacg atatggtgaa agcataagaa
     4321 aggaaaggtg aaaaaatatg agattagcag aaggtattgt gattgaaaaa gaccagacat
     4381 ttggcacatt gaaattttcc gccttgcgtc gtgaggttcg tatccagaat gaagatggta
     4441 cggtttccga agaaattaaa gaacgtacct atgacttaaa atcaaaagga caaggacgca
     4501 tgatacaagt cagtatcccc gcttctgttc ctttgaaaga atttgactac aacgcagaag
     4561 tggaattaat caatcctgtg gcagataccg tagcgacggc tacttttcga ggggcagacg
     4621 ttgattggta cgtgaaagca gacgatcttg tattaaaagg acagacaaaa cctcatcaga
     4681 aatataaaaa agaagcgtcc gaaagtgaaa aataggtgtt gcttatgaca aacacaccat
     4741 tgattttgaa agaaattccg aaggatgaag ccatttcttt tattcggcaa taccattatt
     4801 caaagatact ccctagattg tgtaaatact ttctagggat tttttcagaa gaaaaattat
     4861 taggcgttgt ggaattaggc tggggtacgc aacccctaca aaccatacgc aaactctttc
     4921 ccgacagttc ccttcaaacc acagattatt tagaaattgg aaagatgtgt tttcttccag
     4981 aaatgaatca aaccaattat tttggcagtc aagctctttc agctctaatt aagtggttga
     5041 aagaacatac ggactgtcat tttttatata cactggcaga tggaattgag ggaaaatgtg
     5101 gctatgtcta tcaagcgtcc aacttctttt actgcgggta tttcaagaca agtgtctatc
     5161 gtgataagca gtcatgggaa aagatacacc ctcgaagtgc tcgcctttta ttagaggaaa
     5221 atgctcgctt tgaacaggta gagaaaaagc attggctttc ccaggcattt tgcgaataca
     5281 aagggattga gaaaatcaat ggacgcatgt ttcgctatct ctatccgttg acaaaagaag
     5341 cgaaaaaact gttaggacac actctctatc gacgtcatta ttatccgaaa gaaaaaaatt
     5401 tacgctttga aaagcgaatc gcttatcaga aatatgaagc catttctcaa ccaacttttg
     5461 ataaacaggc tcgtatctac aatacacaat tattttgata ggtggaaagg aggattttta
     5521 tgccattgct tcatgacaga ggagagcgaa tcaaagccaa cgataagcac ttggtctatc
     5581 attttgtact aatttcattg ctggtagttt ttctagtcgt cctactccct tttcattgga
     5641 gagcgttaca atcggtgaac tggcaagcag ttcagttaga tacatttttc tatcaacttc
     5701 atacgccata cttttggagc agtattctca ctgctctttt tgtatgcgga cttatcagct
     5761 ggctttatta tcactatcgt atagattcta taaaacagtt agagcaccgt caaaagttag
     5821 ctcgtatgat tttagaaaat cattggtatg aagccgaaga aactcaatca gagggattct
     5881 ttaaagattt acccagcaag aaaaccaaac aacgaatccg ccattttcct aaaatatatt
     5941 atcgaatgaa agatggacta atccatattc atgtagaaat caccttaggg aaatatcaag
     6001 atcagctttt gcacctagaa aacaaactag aaacaggatt gtattgtgaa ttagtggaaa
     6061 aaacgttgaa agattcttat gtagagtatg tcctacttta tgatactatt ggcaatcgaa
     6121 ttggcattga agacgtggta gctcaagatg gacgtgtcaa attgatggaa tcggtctatt
     6181 gggagttcga ttctctccct cacatgttaa ttgctggggg cactggtagt ggaaaatcct
     6241 attttattct cacattgatt gaatctctcc tgcatacaaa tgcaaagctt tatattcttg
     6301 accctaaaaa tgctgatttg gcagacttag gggcggtcat gaataatgtc tattaccgta
     6361 aagaagatat gttggcatgt atcaatcaat tttacgaaga tatgattgat cgcagtgaaa
     6421 ccatgaaaca acaccctaac tataaaacag gagaaaatta tgcttatctt ggtttgtctg
     6481 ccaattttct tatttttgat gaatatgtgg caatgatgga tatgctaggt cgtgaaagta
     6541 ccaccgttat tcataaactc aaacaaattg taatgttagg acgacaagca ggcttctttt
     6601 taattcttgc ctgtcaacgt ccagacgcaa aatattttag tgatgggatt cgggacaact
     6661 ttaacttccg tgtggctttg ggtcgtatgt ctgaaatggg ctttggtatg atgttcggaa
     6721 gcgatacaca aaaacaattt ttcttaaaac ccatcaaggg aagaggttac gtggatacag
     6781 gaaaaagtgt gattagtgag ttttatacac cccttgtacc aaaaaggtac gatttcttag
     6841 gagaaatagg aaaagtcatt caaaaaaagc aatctgagcc tgtaccgcac gaagtgaaag
     6901 gtacaggttc agacttgctt tgattggtgt ggcgttagcc acgccaatcc tttagccccc
     6961 tctatctaac aggggggcac aaatacaact gaaaacaggc aattctcatc aaccaatgtt
     7021 gatggtacaa aggattgcgg aaaatatgaa aggtgtaaac tcaagggtca tagtttacat
     7081 cttttttaga ttggaggttt gcgaatggaa aacgaaaaaa tttggattaa ggaattgaaa
     7141 gaaaagcgac tggcttatgg cgtttctcaa aataaactgg cggttgcttc tcatattacc
     7201 agaccttacc tatcggatat tgaaacaggc aaggcagtcc ctactcaaca ggtgaaagaa
     7261 gatttactga acgctttgga acgcttcaat cccgataacc cattggagat gttattcgac
     7321 tatgtacgga ttcgattccc taccaatgat gtggctcaaa ttatcggtga ggttttacgt
     7381 ttgaatatgg attatatgct acacgaagac tttggctatt attcttatcc cgaacactat
     7441 cgtttcggtg atattgttgt cctagtctct catgatgtaa gtaaaggtgt tttattagaa
     7501 ctgaaaggaa aaggctgtcg gcaatttgaa aactttctat tggctcaaca tcggagctgg
     7561 tatgatttct ttcaagattg catggaacat aaaggtattt tcaaacgctt ggatttagct
     7621 atcaacgata aaacagggat tttgaatatt ccagatttgg caaagaaatg taaacaggaa
     7681 gaatgcattt ccgtctttcg aagctttaaa aattatcgca gtggtgaatt ggttcaccgt
     7741 gatgaaaaac catacatggg aaacacactt tatattggct cattaaaaag tgaagtctat
     7801 ttctgtatat atgagaaaga ttatgagcaa tacgtgaaaa atgatgttcc cttagaagac
     7861 gcagaagtaa agaatcggtt tgaaattcgg ttaaaaaatg accgagccac ccaagccatg
     7921 aaagatttat tggcacatca acaggcggaa aaaacagcct ttgaaattat caatcgctat
     7981 atccgctttg cagataaaga cgacactaaa cgtcggagtg attggaaaac taatgaacgt
     8041 tgggaatggt ttatcggtaa aaatcgtgga gcattacgcc taaccacaca gccagagcct
     8101 tattcttttg aacgaacatt gaactggtta catcatcaag tcgcccctac attaaaaatc
     8161 gcaagcattt tagatgttct caacggtaca accattatct ccaccatgat tcaggaagcc
     8221 aaactgacag aaaaacatga gaaattaatt gaacaacaac atctagccat ggaggatttg
     8281 attacatgag ccttttaaca aaagaattaa agaagttagg gtttcagtgt ggcattgagt
     8341 ttcaagcgta tatacaaaat acagggaaat atacgtctct cattattgag ggaaaacggc
     8401 aagctggtga taccatctat acctatgatt tttacaaggt gagattttat aataattata
     8461 ccaatcgagt aaccgtctat ggagagcatt taaccccttt tcaattatta aggcgtgtaa
     8521 aaagctatat ctattatcga gaaaaatatc ttaaggaaag gaggacaatc acatgaattt
     8581 tgggcagaat ttgtacaact ggttcttgga taatgcacag tcattggtat tgttagcgat
     8641 tgttgtcatt ggactgtttt taggttttaa acgagaattt tcaaaactca ttggcttctt
     8701 gattattgct ctaatcgccg tcggacttgt gttcaatgca gctggcgtga aagatgtgtt
     8761 attaaactta tttaaccgaa tcattggtgc ttaacagata tttttcatga taaaatagga
     8821 agtatatatt ttgttatgaa aggatttatg aatatgttta caaatgatca atttaaatct
     8881 cttttaatta aaacattaga aagaaagagc tcttcaaatt attatgaagg aggtaacgag
     8941 attgtttcta aaatgaaaat atcagaggta actttagatg agactgacga ttttacttat
     9001 tataaaggat ataaaaaggg ctggaatacg ctttgcacat atcttaaaat aagagttcct
     9061 tttgatgatt tagacttttt tgaaagtcat aaagatttga ttacccagac agcaagctcc
     9121 atatatgata aacaaggtga taatgtatta gtggatacta ttttagtacc acttccagag
     9181 aattacgaag taattaactt tagtcagttg aaaattagtg atgtggtttc acaagctatt
     9241 gaagacgctg agtcttttat gagtaatggt gaatatcaac gtgcctttga cagagttcac
     9301 actgcttttc atggatattt aattgaaata ctaaaaaaat atgaaattac agttcctaga
     9361 gacgaaaatc tatctaaatt atatagtagg atacaacaat taattgaaaa agaaattcaa
     9421 cctactgaac ttgctgatat agtgaagaca acgataaggt catcaaatgg aatgattagc
     9481 tctcttaatg aagcaagaaa taggcattca cttgcacacc ctaatacaaa tattattgga
     9541 aagagagaag caaaactaat aataggtatt tcaagtacag ttacagatta tattagcgga
     9601 taccttgata agtagcgtca ttaactgatt gctacttttt ttatgactat ttttataaaa
     9661 aggatgtgat aagaattgga aatgaaagtc tatgtggcaa atttaggacg gtataatgaa
     9721 ggtgaattag tcggagcatg gtttactcca ccaattgatg aggaagaaat ggcagaaaga
     9781 atcggtttaa atgaggacta cgaagaatat gcaatacatg attttgagct tccttttgat
     9841 gtggacgaat acacgccgat tagtgaaatc aaccgtctat gtgaagccat tcaagaaatc
     9901 gaaggtacac ctatttataa tgaactcaaa gaaattcaag gcatgtggtt tagtagcttg
     9961 gaagaattac tggaaaacaa agaggatatt cattgttata gtgattgtga ttcgatggaa
    10021 gatgtcgccc gttattacgt ggaagaaaca ggacagcttg gtgaagtccc ttccaattta
    10081 caaaattata ttgactacca atctttggga cgtgatatgg aaatagaggg taattaccta
    10141 gttacctctc atggggtatt tgaatattgt cagtaagttt aacaatccgt gttataatta
    10201 aattaataaa tagcacggag aggtataaat tatggtaaat gtatttgaaa atcttaaact
    10261 attaaaacta gacatgaaag ataaaggctg ggtgatagat tccttttact ttcgttacaa
    10321 acagcaaaat tatattgtac ttgttaagct atttgaaaaa gaggaaaaag ttccagaata
    10381 tgctctgtta aaactagaat ttttaaagga aaacgatttt tcagatatgc tagctgtcta
    10441 tgctaattcg gtaaaattat atacagacac gaaaacaata agagaatatt ttggtatcga
    10501 atacagtagt aacttgggag atgttttatt tcaatttagc caaactcttg ctcgatttat
    10561 tccgacagaa gttagtgaga agaaaaatga agaccaaaaa gaagccatgt gtttctcatt
    10621 aagtcaaagt gattcagaag atcccagaaa aaaatattgt ttttcagtta gaagaaatcc
    10681 tttaaaggga aatggtgagc tgggaaaacg aagtcctttt aatgacaata aaacgagatt
    10741 gtatagaccc agcttatatg aacgattagg gtcagatacc aatttaagtt tcagatattc
    10801 aatgaatcca gatgatgaag aaactgatga aggtattatt gcaaaatgga caaaaaataa
    10861 aattgaatag ttaaagcatt gaaagagcaa tcacctattt tgattgttct ttttctattt
    10921 ttacggaaag gacgtgaaga catgaaaaaa ataagaagtt acaccagtat ttggtcagta
    10981 gaaaaagtga tttatgcgat taatgattta caactccctt tcccaattac ctttacgcaa
    11041 atgacatggt ttatcgtttc tttgtttgtc gtcatactct ttggcagttt accaccactt
    11101 tctctgattg acggagcact actcaaatac ttagggattc ccattgttct cactgggttc
    11161 atgtgccaaa agacctttga cggaaagaaa ccctatggat ttctaaagtc tatcattacc
    11221 tatgctctac gccctaaagt aacctacgct ggcaaacaaa tacgattaaa aaaagaaaca
    11281 atgaatgaat cgattacgat tgtaaggagt gaacaactag atggcatatc cgattaaata
    11341 tatggaagat aatctcgtat ttaatcatga tggcgaagta ttcgcttatt atgaattaat
    11401 tccctataat tacagttttt taagttccga tgaaaaaatt caagttcatg ataatttctg
    11461 tcaattgatt gcccaaaatc gtgatggcaa gattcatgct ctgcaactag caaccgaatc
    11521 aagtattcga gccacacagg aacaatcgaa aaaagaaatc actggacgat taaaagagat
    11581 agcttacgaa aaaatagatg aacagaccga agcgttaatt tctatgattg gtgacaatca
    11641 agtagactat cgctttttta ttggttttaa actctacctc aatgaacagg aagtatccgt
    11701 aaaaaatgtt gggaaagaaa ttcaaaaagc atttgcggaa ttctttcatg gtgtgaatca
    11761 caaattaatg ggcgattttg tttctatgcc tatggcggaa atcgaacgct tttctaagat
    11821 ggaacagttg ttagaaagta aattatccag acgctttcag attcgtaaat tggagaaaga
    11881 tgattttggt tatattattg aacatcttta cgggcaaaca ggtgtccctt ttgatgacta
    11941 cgactaccat ttaccgaaac gatacgaaga taaaaaagtg ttggtaaaga aatacgatat
    12001 tctcaaaccc actcgctgtt taatcgaaga aaatcaacgc tatttacgga ttgaacagga
    12061 agataccact acttatgtgg cgtattttac aattaatagt attgttggtg aattggattt
    12121 ccctaatagt gaaatttttt attaccagca acagcagttt actttccccg ttgataccag
    12181 tatgaatgtt gaaattgtcg ccaataaaaa agccttatct accgttcgca ataaaaagaa
    12241 agaactgaaa gatttagata gccacgcatg ggaaagcgac aacgaaacca gtactaatgt
    12301 ggtggacgct ttagaatctg tcgatgaatt agaaagtaat ttagaccaaa ccaaagaatc
    12361 tatgtacaaa ctaagctatg tggtacgggt atcagcaaag aatttggacg agctgaaaca
    12421 acgttgtgat gaagtaaaag atttttatga tgatctgtct atcaaattag tacgaccttt
    12481 tggcgatatg ctaggcttgc ataatgaatt tattccctca agcaagcgat atatcaatga
    12541 ctatattcaa tatgtcacgt cggatttttt ggctggtctt ggtttcggtg ctacacaaat
    12601 gattggtgaa aattctggta tttacgtcgg ttacaatctc gatacaggca gaaatattta
    12661 tctccaacct agccttgcaa gtcaaggaat taaaggctct gtaacaaatg cattagcgtc
    12721 cgcttttatt ggttcattag gtggcggaaa atcattctct aacaatatgc tggtctatta
    12781 ttcagtactc tttggcggac aggcaaccat tgttgaccca aaagctgaac gtggtgaatg
    12841 gaaagaaacc ttacctgata ttgcccatga aatcaatatt gttaatctca cgagtgagaa
    12901 cgacaataaa gggttacttg acccttatgt gattttaaaa ctcccgaaag attcagaaag
    12961 ccttgccatt gatattctca cgttcttaac aggtatttcc agccgagata gcgagaagtt
    13021 tcctgtcctg cgtaaagcta ttcggagtgt aactcaaagc aaacaacgag ggttgttatt
    13081 ggtgattgat gaattgcgaa aagaagatac gcccatcagc aatagtattg ctgaccatat
    13141 cgaaagcttt gtcgattatg attttgccca tctgttattc agtgatggca gtgtcaccca
    13201 atccattagc ttggaaaaac aactcaatat tattcaagtg gcagatttgg tcttacccga
    13261 ttccaatagc tcttttgaag aatacactac catggaatta ttaagtgtgg caatgctgat
    13321 tgtcattagt acctttgctt tagactttat ccactctgac cgttccattt ttaaaattgt
    13381 tgaccttgat gaggcttgga gttttttaca agtggcacaa ggaaaagctc tatctatgcg
    13441 attggttcga gcaggacgtg ccatgaatgc aggggtctat ttcgttactc aaaacgcgga
    13501 cgatttacta gatgaaaaga tgaagaataa tattggcttg aaatttgcct ttcgttccac
    13561 tgacctagtg gaaatcaaaa agaccttaga attttttggt gtggacaagg aagatgaaaa
    13621 caatcagaaa cgcttacgtg aattagaaaa tggacaatgt ttagtttcag acatctatgg
    13681 tcgtgtaggg gttatgcagt ttcacccgat ttttgaagag ttgcttcatg cctttgatac
    13741 cagaccgcct gtgcgaaatg aggtggaaga atgacacaaa aacaaaagaa atttcttcac
    13801 tatagttgga taacgctcct ttgtgtgggc gttattttac ttttattagg cacgctgggc
    13861 aatgcagtac aagcgacagg cttagttgat gaaaccattg atacaagcaa tgaatactcc
    13921 aagtatggtt tgaatcaata tcagcttgat tattatgtgg acaatagctg gggctggctt
    13981 ccatggaatt ggagtgatgg cataggacaa tcggtgatgt atggattgta tgccattact
    14041 aattttattt ggacaatttc tctttatcta tcgaacgcca caggttattt ggtgaaagaa
    14101 gcctatgcct tagatttcat tagtcaaaca tcccaagcaa ttggcgaaaa catgcagacg
    14161 attgcaggca ttacaccgaa tggcttttcc agttcagggt tttatgtcgg tttcctcctt
    14221 ctttttattc tcattgttgg aatctatgtt gcctatacag gattggttaa gcgagaaacg
    14281 acgaaagtga tacgagctgt tttaaacttt gtcgtggtgt ttattctatc ggcttccttt
    14341 attgcgtatg cacccgatta tattgcgaaa atcaatgatt tttccaaaga tgtcagtacc
    14401 gccagtctga atattgggac aaaaattgtt cttcctaaca cggatagtca agggaaagac
    14461 agtgtggaca tgatacgtga cagcttattc tctgttcaag taaagcaacc atggctattg
    14521 cttcaatatg gctccactga tgtagaagcg ttaggggaag aacgtgtgga aagtttattg
    14581 tctaccagtc cagatacaaa caatggtgaa gatagagaaa atgtagtgat tgaagaaatt
    14641 gaagatcatg acaataccta tttaacccta ccgaagacga tttccagatt aggaacagtc
    14701 ttctttttgt ttgtattcaa tattggtatc tcgatttttg ttttccttct aacaggaatc
    14761 atgatttttt cgcaagtgct atttattatt ttcgctatgt ttttgccaat cagtttccta
    14821 ttaagtatgt tgccgagttt tgatggcatg ggcaggcatg ccattaccaa gttatttaat
    14881 gtgattatga caagggctgg tattaccttg attattaccg ttgcctttag tatttccact
    14941 atgctttatt cgctgtcagc aagttctccc tttttcatga ttatgttttt acaaattgtg
    15001 acctttgcag ggatttattt taaactgggc gatttaatga gcctattcac tttacaaagc
    15061 aacgactctc aaaacatggg cagacaagtg tttcgcagac ctcgtcaaat gatgaatcga
    15121 caaaccagaa gatttcaacg cagtatgagt aagatttttc gtggaaaagc agggtcaacg
    15181 aagcaaaaga caatgacaac aaagcagaat caaaccaata aagctaacca tactcgtcaa
    15241 aatgaacgga ctacaccaaa gaaagaaaca gcactttcca atgtgaaaaa gcaacaaggc
    15301 ggtatcgggc aacgattagg tgaaaagaca gcccatgtat tggacacgaa aaatcgtcta
    15361 gtcaataaaa gaaagcaggc aaaacaacag atacaggcga caccaaccca tgtcaaatat
    15421 gcccttcatc aaggaaaaga gaatgtcact cataatgtaa cagactttaa acaatctatg
    15481 gcagatacca aagctcaacg caaacaacag cgtatgaacc agcaggagca aaaacgaaag
    15541 aacattgctc aaaagcgttt ggagctggat aaagcaaaag aaaaaagacg aaaagaaaac
    15601 ccatcatctc ctgttcagaa taaccagcag catgttcgca agcgtcagca aaccgtcacg
    15661 cctgttttgt cacaaaacac taaacgtgaa aatcatcagc gaccattgaa aaaagagcaa
    15721 aaacaaaaga caccaccgaa gcaattcaat cagaggaagc taaataaaag aacggataag
    15781 cgaggacaac aaacatgaag ctaaaaataa cagtgatttg tagtgtggtc ttctctctct
    15841 tcttttttct cttattattc ctagtcattt tctttgcaga tgatacggac agtggcgaaa
    15901 acaacaagga ttcttctatt ccacaaggcg gtgtgaccgt ttcaccagaa gtgttggcac
    15961 accgtccgtt gattgaaaaa tatggtaagg aatatgggat tgaagactat gtttcttata
    16021 tcttagccat tatgcaagtg gaatcgggcg gtacagcaga agatgttatg caaagttccg
    16081 aatctttggg tttaccgccc aacagtttga gtaccgaaga gtccatcaaa caaggggtta
    16141 aatatttcag tgaattatta accagtgcag aacaacaagg agtggatata gattctgtta
    16201 tccaatctta taattatggc ggtggctttt taaattatgt gaggagtcat gggaaaaaat
    16261 atacctatga attagccgaa cagttttcta aagagaagtc gggtggtcaa aaagcagact
    16321 atcccaatcc tatagccata cctgtgaatg gtggttggcg atataactat ggaaatcagt
    16381 tttatgttca gttggtttca caatacctaa ccgacacttc cccaacagaa tttgatggtg
    16441 aaaccgtgca agtcattatg gacgaagcct tgaaatacga agggtttccc tatgtctttg
    16501 gaggggcgtc gcctaccacc tcttttgatt gtagtggctt aatacaatgg gtgtatgaca
    16561 aagctggcat ttctctccct cgtgtcgccc aagatcagta tgacgctacg caagaaatct
    16621 ctatggaaga agcccaagca ggcgacctta tcttctttca ttcaacctat aatgcaggca
    16681 catatgtgac tcatgtggcg atatatttag agggaaatcg cttttatcat gcaggcgacc
    16741 caatcggtta tggcgactta agcagtcgtt attggcaaga ccatctgatt ggggctagac
    16801 gtgttattca taactaagaa aggaatgaat caaatgatta taaaaatcga gcgaaaagaa
    16861 aagaaggaga aaccaaagaa atctaaaaaa ccgagcaagc aaagaaaagt tccgatgatt
    16921 aaagttggca cgcataagaa attaaccttt gttttatggg ttttattgat tggtagtgtc
    16981 ggatttggaa tctataaaaa ttttactgcc attgacactc atactgtccg tgaaacagaa
    17041 atcattaaac agcagattgt cgataccaac caagtagaaa gttttgtgaa atcctttgcg
    17101 aaagactact tttcgtggca acaatcccaa gaagccattg ataaacgaaa tgaaaaattg
    17161 acccattatt tgacggaaga attgcaggta ttaaatgaag aaatgattcg taaggatatt
    17221 cctactagtt ctagtgtgaa cgatatacag gtgtggcagg tttctcaagt gaatgagaac
    17281 acctttgagg tactgttttc tgttgaacaa gttattaccg aagacaaaga caaggaaact
    17341 atatcgtcta gctttcatgt ggttgtccat atagatgaat cagataatat ggttattatc
    17401 aaaaatccga caatgagtaa gaaacctcaa aaatcggact accaacccaa acagctagaa
    17461 agtgaccata cagtagatac agaaacgatg gacgaaatca ctagcttttt agaaacgttc
    17521 ttccagctct atcctaccgc cacagaaaaa gaattgactt actatgtaag taatcatgtg
    17581 ttacctatga ttaacaagga atatgttttc gaggaattgg tgaatcctat ctttaccaga
    17641 aaagataacc aagtaatcgt gaatgtcgct gtgaaatatt tagatcaaga aacaaaggca
    17701 acccaaatct cgcaatttga gttgatatta gaaaaacaag ataattggaa gattgtaaaa
    17761 taacaaatat tggtacatga ttacagatac tttgtaatca tgtactcttt ttgataaaaa
    17821 attggagatt cctttacaaa tatgctctta cgtgctatta tttaagtgac tatttaaaag
    17881 gagttaataa atatgcggca aggtattctt aaataaactg tcaatttgat agcgggaaca
    17941 aataattaga tgtccttttt taggagggct tagttttttg tacccagttt aagaatacct
    18001 ttatcatgtg attctaaagt atccagagaa tatctgtatg ctttgtatac ctatggttat
    18061 gcataaaaat cccagtgata aaagtattta tcactgggat ttttatgccc ttttgggttt
    18121 ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc
    18181 aggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg
    18241 aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat
    18301 tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga
    18361 cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg
    18421 ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca
    18481 tgcacttagg aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg
    18541 aattgattta tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat
    18601 caaacagaag gtagaactgt atcctaatat gtgtgtgacg aactttaccg aatctgaaca
    18661 atgggatacg gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc
    18721 attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt gttctctgtt
    18781 ccctctttat catggaagtg caaaaagtaa tatagggatt gataacctta tagaagttat
    18841 tactaataaa ttttattcat caacacatcg aggtccgtct gaactttgcg gaaatgtttt
    18901 caaaattgaa tatacaaaaa aaagacaacg tcttgcatat atacgccttt atagtggagt
    18961 actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag ttacagaaat
    19021 gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg gagaaattgt
    19081 tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa aactattgcc
    19141 acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg aaccgagtaa
    19201 acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata gtgatccgct
    19261 tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt
    19321 acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa
    19381 agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat
    19441 cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt
    19501 gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcatttca
    19561 aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa ggattatatg gttggaatgt
    19621 gacggattgt aaaatctgtt ttaagtacgg tttatactat agccctgtta gtactccagc
    19681 agattttcgg atgcttactc ctattgtact ggagcaagcc tttagaaaag ctggaacaga
    19741 attgttagag ccatatctta gttttaaagt ttatgcacca caggaatatc tttcacgggc
    19801 atataacgat gctcccaaat attgtgcaaa tatcgtaaat actcaactga aaaataatga
    19861 ggtcattatt attggagaaa ttcctgctcg atgtattcaa gattatcgca atgatttaac
    19921 tttttttaca aatgggctta gtgtttgttt agcagagcta aaaggatatc aggttaccac
    19981 tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg atagataaag taagatatat
    20041 gttcaataaa ataacttagt gcgttttatg ttgttatata aatatggttt cttattaaat
    20101 aagatgaaat attctttaat atagatttga attaaagtgg aaaggaggag attgttatta
    20161 taaactacaa gtggatattg tgtcctattt gtggaaataa aacaagacta cgaatacgag
    20221 tggatactat acttaaaaat ttccctttat actgccccaa atgtaagaac gaaactttaa
    20281 ttaatgttca aaaaatgaat ataataacaa tcaaagagcc agacgccaag acgcagagcc
    20341 gataatttga gaaatgaaac tctcatctta tcggctcttt ttgtttatct gaattttact
    20401 gactagcctt caatatttct tgcttcattg ataccttttg ctagtgcttc cattaaggat
    20461 agttctttgt ctgtaaagct atccatgtat ttttctacct gtaaccttcg agtgctttta
    20521 atcaaattat ctgtaggtaa gaaaaattca tcaatagata catggagtaa tgatacaaga
    20581 tcatacagca cttgtgtact tgggtgttgt cctttgtttt ctatatttgt taaataacgt
    20641 gggtcaattt caatcattgt tcccacttgt tcacgagtga gaccccgttt cattcgagcc
    20701 tctttaatcg ctaaaccaac ggctctaaaa tcgtatttat cttctttttt acgcatacta
    20761 aatcacctct atacatttta ctgttcctat ataaccataa acaggtatag aaaaacatgt
    20821 tatatagttt atgagttcct atagaacact gtggaattta ttaatgcgat gaatccaatt
    20881 tcaaatagaa aggtcgctga ttttatttac ctatacaact ttataaaatt tagtcatact
    20941 cctagaaagt agataaaatt ggcgacttaa tggagggaaa agtcgccatg agaaagataa
    21001 tctgtcatct agttctctta attaagttct tatcaaaaaa acttatccac ctgtactgga
    21061 tagatatgac catcaatgtg gttttgcaga ttacatagat agaaaaatca aagcatatga
    21121 acagagaaaa caatctgttt atgtgctttt ttggtcactt aataaatatc ttttaggatt
    21181 tctcttcatt tatgcaacaa atcccccttt cgtgtcgggg tttagtatga agggagataa
    21241 agaagcacat gccactccct ttcacgacag aaagggggtg aaaagcatga aaccatcttc
    21301 ttttcaagaa accatagaaa atcagtttga ctatatctgc aaacaagtta ttgaagatga
    21361 acgaaaagat tacttcaaac atctagacag actatcagca aaagaagtct cgttttctga
    21421 tatagataac taccttgtca actctttttc tactgttgat acgtacgtca ctgatttcca
    21481 actatttaaa ttatttggat taagaatcaa tgttgaaagt gatttattaa gtgaagccct
    21541 gcgtcatctt tcagagaaaa aacggaacat tattttactt cattacttta tggatatgag
    21601 tgatgtggag attgcggagt tactaaattt gaatcgttcg actgtttatc gtcatcgaat
    21661 aagtggacta gccatgatta aagaattcat gaaggagtgt aaagaatgaa aacagcttat
    21721 ccaagggttc ccttccccct cattgtaaag gcgacagatg gcgacgtgga agcaattaat
    21781 cagattgtta aacattatcg agggtatacc tcaaaacgtt cattacgtcg catgacagac
    21841 gaatatggaa attctcatat ggttattgat gaaaccctac gtggcagaat ggaaacgaaa
    21901 ctcatctcta agattttatc gttcgaaatt aaatagtccc attttttcgt ggaagcgtgt
    21961 cgaatcgtat ccacgcttcc cgatttggga ggtttgttat tccatcaaag catattgtgt
    22021 gttcagtagg ttttgatagg ctaacaagcc aaacgttctt tgacaaccaa ataaaggcaa
    22081 tcgaatacgt ttcggtaaga aaagagctga tgaactaaca cgccatgacc tacaaaatta
    22141 gtgagcgatt catgttagta attcgaaagt aatctttagc aggattgctg ccatgacttt
    22201 cttatcgtga taatgatact cccgtatagc catagtccga gcgttagaag cgtcacaggc
    22261 aatgggtacg gctacatgag aaccatgcag aagtgaaatt cctgtgagct ttgctagagc
    22321 tgttcgattg ctaagatatt cttctcttgt cagtgtatta ttataaaact atttgatata
    22381 attataggtg aatattggag ttttcctagc ctataattgg gaaaggagta ataatgataa
    22441 cggtcacaaa aaaggattta gttgagctag gatatggaac ttctttttca gcagacatca
    22501 taaaaaaagc aaaagaattg atgattacaa aagggcatat ctactatcaa tctcgaaagc
    22561 tagatagagt ccctcgtgaa gctgttgaag aaattctagg aattcggttt tcaaatgaaa
    22621 acaggtctga gtagtttttg cacagtaagg agtaaaaact atggttaaag acccaataaa
    22681 aaaagcaaag aatggaacct attattttcg tgcaaatcta ggttatgatt ctaaagggaa
    22741 aaaaattcaa aaatatcgta gtggattcac tactaaaaaa gaagcaagag aagaatattc
    22801 gaaactgcta ttgatgaaac cagaagaatt aagtgagaat aaggataaaa tgacgtttaa
    22861 acattatatc tttgaaattt tccttccatg gtataagaca caggtaaagc tcagaacata
    22921 tgaaaatcgc ttaccaacta ttaagaagca tttctcttat tttgataaaa tggctgtttc
    22981 cgatattgag cctattgatg tgcaaaattg gcaactaaaa ttatcaaaga agtgtaagtc
    23041 ttcttatgta agagctgttc aaggactatt ttcagttgct atggatcgag caattgtcct
    23101 tgggataacc accacaaatc catctaagat tattggcaat gtgaaaaagc aaaagtctaa
    23161 aattgaattt tggacaaaag aagaatttga aaaagtaatc tctctaattt acaaagaaga
    23221 ttactatcaa cactttttat ttatctctct gtggttttta tttatgactg gaatgcgaat
    23281 tggtgaagct actgccattc aatgggaaga tattgatttt gactcaggag tactaaccat
    23341 caacaaaaca ctctattaca agaatcaatc gaattataga tttgttgaac caaaaacaaa
    23401 ggcgagcgtt cgtcatattg tattagataa atgcacatta acttatttat ccgaatggaa
    23461 ggaagtacaa caaaatctaa ttcagacaga ctttgtaatg agctataacg gtattcctac
    23521 ccaaaaacat acactatcat atgctattga acgctatgct aagctggctg gtatccatcg
    23581 aatcagaatc catgggttac ggcattctca tgcttccttg ttaatcagta tgggagaaaa
    23641 tccacttatc attaaagatc gtctaggaca cgaagatatt gagacgactt taggaacgta
    23701 tggacatctt tatcctaaca gtaatttcga ggtagctaac aaattaaatg gagccatttc
    23761 ctttaaagaa gcaaaacaaa attatgatac ttcaccaaag aatcaattta ctgtagacta
    23821 cttgcgaaac aaagaaatga aaaaagtgca ataaaagtgc agtaaaaaga taagaagagc
    23881 cagaaaccct tggtataaga ggatttctag ctctcctaca actattccca ctccactgtt
    23941 gctggtggtt tagaagtgat gtcatacacg atacggttga cgtgggctac ttcgtttacg
    24001 atacgtacag agattttttg caagacttcc catgggatac gggcaaagtc agcggtcatt
    24061 ccgtcgattg aagttacagc acggataccg actgtgtagt cgtaagtacg tccgtcaccc
    24121 ataacaccga cagagcggat ccctggaaga acagtgaaat attgccagat atcacggtca
    24181 agtccagcag ctgcgatttc ttcacgcaag atagcatcag attcgcgtac gatttctagc
    24241 ttgtcttcag tgatttcgcc aagaacacga ataccaagac caggaccagg gaatggctga
    24301 cgccaaacga ttgaatcagg catgcctaac tgagtcccta attcacggac ttcgtctttg
    24361 aataatgtgt ttaatggttc gatcaattca aattgcatat cttctggcag tccacctaca
    24421 ttatggtgag atttgatcgt ctgtgctgtt tctgtaccag attcgataac gtcagtatac
    24481 aacgttcctt gtgctaggaa agagacgcct tcttcaccag caagttttgt tgcttcgtca
    24541 tcaaaaacat atacgaattc attcccaatg attttacgtt tttgttctgg gtcagataca
    24601 cctttcaact tgtctaagaa gcgatctctt gcatcgactt tgatgatgtt caagccgaat
    24661 tttccgccta gactttccat tacttgttcc gcttcgcctt tgcgtagcag accgtggtca
    24721 acaaagatag atgtcaattg gtcaccgatc gctttttgta aaagaacccc aacgacacta
    24781 gaatcaacgc cacctgaaag accaagcaag acttttttgt caccaacttg ttcacggatt
    24841 ttttctactt gcatatcgat aaagttatcc attgtccagt taccttcaca gtgacaaaca
    24901 tcaaaggcaa agtggcgcag caattcgatt ccgtattcgg aatgacgtac ttctgcatgg
    24961 aattggatac cataaaaatt acgtgttgtg tcttggatag aagcaatcgg acaatctttg
    25021 cttgttccga ctgtttcaaa gccttcagga actttagtta ccaagtcacc atggctcatc
    25081 catactgttt gttgttttgg tgtttcttta aacaagacag cttgatcgct tgttacttct
    25141 aacaaggctt tcccatattc gcggttttta gcaggctcta cggaaccgcc aagtttgtac
    25201 gtgatcaatt gcataccata gcaaatccct aatacaggaa tacctaaatt gaagatctca
    25261 ggatcgatcc caaatgcatt ctcatcatat acactgtttg gtccgccaga aagaatgatt
    25321 ccctttggat tcatcgcttt tacttcttct gcagtaatgc ggtggctcaa taattcggaa
    25381 aagactccaa gatcacgaat acgtcttgtg atcaattggt tatactgact accgtagtct
    25441 aatacgataa tcttttcaac gttcgtcata tcggcaacgt ttgtcaaatt tattacccct
    25501 atccctttaa attttatctc aaaatagtca aactatttta aaatgctaat attttcgcat
    25561 gaaaatcgaa tcgatgatat gattttcagt tttatggaga attatatcgg ctcttcctcg
    25621 tgtaggcaag atatattcct gtaaattttt caaattcaca tttttccata catcttttgc
    25681 cattttaaag gcttcttcac gatctccgat agcaaaacga taataataat tctctggatt
    25741 ttggaaagcc gtatccagta acgcaccaaa acgatccaaa taccattttt cgatcagcac
    25801 gggatcggca tcgacataga cagaaaaatc aaaaaagtcg ctgacgtaga tttgctgatt
    25861 cgcaggtaat tgaagcgtat tgatcccctc aacgatcaag atatcaggct gctggatcaa
    25921 ttcgtacttg tctggaatga tatcatacac atcatgtgag taaacaggta cttttatctc
    25981 ttcctgacca gattttacac ggttcaagaa atcgatcagc atctccatat cataactttc
    26041 aggaaatcct ttacggtcca tgattccacg ctcttttaat atacgatttg gataaagaaa
    26101 tccgtcagtc gtgatcagct gaacgtttct tcgtttgaag gtacgagaca aaatcgtctg
    26161 cagcagtctg gctgttgtac tttttccaac ggctacactt ccggcgatcc cgataataaa
    26221 aggcggtact tctacgtatt tatgaagaaa taaaccttta ctcaagaaca aagattcaaa
    26281 ttctttcata taaagataaa tcagatgagc aagaggtaca tagatatctc gaacatcctg
    26341 catggaaatg cggtcattca aacttttgat attatctagt tcatcttgtg tcaaaggtgc
    26401 ttgcccatta cgataaaatt ctcgccattc ttctcttgcc acacggtagt agttcattgg
    26461 atcattcata ttagcctcct gcgttctccc atcaatggaa ctattttaac acaaaataaa
    26521 cgcaaacccc accacttaag agagattgcc gatctttttg tataatcttt ataatagaaa
    26581 gaaacggagt cttattcttg acttgcgcac ggatacataa aaggatagac tgttgtcatg
    26641 aaagaaatga gaaggatgga agacatgaca aatcattact attctgaacg cccagatacg
    26701 gcgcacgatt ttgaacaatg ggcttttgag ttaaaaggca gaacctttca atttgtaaca
    26761 gattctggtg tattttcacg tgaaacagtg gattatggtt cacgtgtcct gattgatgct
    26821 ttcgattggg aagagttacc tgatgaagga aagatcttag atgtgggatg cgggtatgga
    26881 ccgattgggt tagctttggc atttgcgaca cagcggtttg ttgaaatggt cgacattaat
    26941 gaacgagcgg taagtctagc gcaaggaaac gcaaagcgta accaaatcga acatgttgat
    27001 atccatcaat cgaatatcta tgaagcagtt catgaagaaa cctatgcagc tatcgtaagc
    27061 aatccgccga tccgtgctgg aaaaaaagtt gtacatgaaa ttttgacagg cgcatatcct
    27121 cggttgaaga agggcggtac actgacaatt gtgatccaga agaaacaagg agcgccgagt
    27181 gcgcaaaaga aaatggaaga aacatttggt aatgcagaaa tcgtcacaaa agataaagga
    27241 tattatatca tcaaaagtgt gaaagaataa ctagctgaaa aatagtggag cgacgaaaga
    27301 gtggaactgg gcgtgcatgt acgggtacgt atcagttctt tttttgttga gaagttttta
    27361 aaaacaaaga actatttttt cttcaaaaag aaatagtagg aacagagtcg ttgtttttta
    27421 cgataaaata aataagatac agaacttgta tagaaggctg ccaagtcggt agataaagga
    27481 ggtttttatc aaaaaacata ctttcctttt ttctctattc ttttcttggg ggttttggta
    27541 cactgatagg gaatttagat agaagggagc caataggatg aagctgacca tcagagatat
    27601 cgcagaaatg gcaggcgtat cagtaacaac tgtctcacag attttgaata acaaaggaag
    27661 cagatttagt gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa
    27721 gcccgattat ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg gaatgatcgt
    27781 ccctgatgtc actgatttct tcttctccaa actgatcgaa ggtgtagaga gctatttgaa
    27841 tcctttgggc tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa
    27901 gtatttacaa gagctttccc accgctcagt ggacggaatc ttgctggcca caccaaatat
    27961 tttacctgag aaatatgcat tagacagcgg tttttatcaa aaaatgccaa tcattttaat
    28021 cgatcgcgga ttgaatcgtc gggataacgg acggcttatc gtaaaggaat atgagggcgc
    28081 ctatcaagct gtttcgctgt ttattgaaaa tggccatacg aagatcggca tgctgaagga
    28141 aaccactgga tactatcaat tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga
    28201 tcatggcctg ccttttaatg ggaaatttgt tgaacatgga gagttgactg tccaaggtgg
    28261 atatactgca tcaaaaaaat tactcaaaca taaagatatc acggctattt tttgcgggaa
    28321 tgatgccatg gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga
    28381 agatatctca gtcataggat tcgatggatt aaagctgtca gaatacatga ttccgcaatt
    28441 gacgacagtg cagcagccaa gttttgatat tggattttat gctgcacgtt tcctgatcga
    28501 tacgatcgaa tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat
    28561 tcgtgagagc gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc
    28621 atttacaaag gctagttagt gcttttttta tttttagtaa accagtttac taaagtggtt
    28681 tacctttcct aaaaataatg tataataccg aatgaagaca aggaggatca ggcaatgcga
    28741 atggtggatc tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag
    28801 tacatcgtca cgaactatac gaatggaaag attccagatt atcaagtcag tgcattattg
    28861 atggcaattt tttatcaaga catgacaaat gaagaaatca cgaacttaac attggcgatt
    28921 gctaattctg gtgatgtcat cgatttgagt tctcttgaag gaataaaggt agataagcat
    28981 tcgacaggtg gcgttggtga cacgaccaca ttgattttag cccccctagt agctagtgtc
    29041 ggagtgacag tggctaaaat gtctggacgc ggtctaggtt acacaggtgg tacactagac
    29101 aagctagaag caattcccgg atttcagatc gagctttcgg atgaagaatt tgtacgcatt
    29161 gttaatgaaa gtaaagtagc ggtaattggt caatcaggaa acttggcacc ggcggacaaa
    29221 aaattgtatg ccttgcgtga cgtcacagcg accgttgatt ctcttccgct gattgccagt
    29281 tcgattatga gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga tgtgaccact
    29341 ggagacggtg ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg
    29401 agcattggga aactggctaa ccgagaaaca gtggcggtta tctcagatat gtcagaacct
    29461 cttggcgaag ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac attacaagga
    29521 aatggtccag aagatctagt agaaatgtgc tacgccttag gaagccaaat ggttgttctt
    29581 gctggcaaag ctaagacaac cgatgaagca agaacgttgc ttcaagaagc tctggaatca
    29641 ggtaaagcgt tagcaaaatt caaagaaatg atccaaaatc aagggggaga cccagcgatc
    29701 gtggaacatc cagaacgaat tttgactgca cgctacacaa tggagcttcc tgctaaacaa
    29761 agcggagttg tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt
    29821 gctgggcgta aaaccaaaga agaagatatt gatcacgctg ttggattgaa actgcataaa
    29881 aaaattggtg acactgtgac aaaaggtgag tcactattga cgatctatag taacgacgaa
    29941 gaaatcactt ctgtcattga attattatat aaaaatattg aaattggtga atcggcaatg
    30001 aagccaacgt tgattcatga catcatcaca gaataaagga aaaaagcgga ctttaccgtt
    30061 ttaaagaaaa ggggaaaaga aatggaacta aaccgaatga tcgaccatac tattttgaaa
    30121 gcagacgcat ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt
    30181 tattctgtct gcatcaatcc agcatgggtt gcgctagcga aagaacaatt acaaggtgag
    30241 ccagtagccg tatgtacagt aatcgggttt cctctaggag caaacacacc cgagacaaaa
    30301 gcatttgaaa caacagatgc catcaataac ggtgcagatg aagtcgatat ggtcatcaat
    30361 atcggtgctt tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga agcagttgtt
    30421 gaagcagcaa aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt attagataga
    30481 gaagaaatca tccgagcttg cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca
    30541 tcaactggtt tctcaacagg cggagcaaag gtagaggatg tccgtttgat gcgtgaaact
    30601 gtaggaccag aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga agaagcaatg
    30661 gcaatgatcg aagctggtgc tacaagaatc ggaacaagcg caggagtagc aatcgtatct
    30721 ggttcaactg gtgaaggcta ttaatttttt caaaggagaa gctcatggca gaactcaaac
    30781 aagaatggat caacacagct attgaagcgt tagataaagc ctatgttcct tattcccatt
    30841 ttccggtagg cgcttgtctc gtaacggaat caggcaagat ttatcaaggc atcaacattg
    30901 agaatgcatc atttggatta acgaattgtg ccgagcgcac agcttttttc aaagcagtat
    30961 ctgaaggaga aaaaacattt acgcatctag tcgtagcggg gcacaccccc gatcctatct
    31021 ctccttgtgg tgcatgtcga caagtaatgg cagaattctg ttcccctgat atgccggtta
    31081 cgctagtagg agacaatggt gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg
    31141 catttactga aaaagatcta taactcatgt tcattcagtc agctgactgt tgaaaaataa
    31201 aacaattatt ttaggaggct ttacagaaat gaaaaaagca aaattatttg gtttaggtgc
    31261 tgtcgcacta gcagcagggt tatttttagg tgcatgcggc aacaacggct ctactgattc
    31321 atccggtggc aaaagctcat cagacacaac aactgcagca ttgatcacag acacaggtgg
    31381 tgtagacgac cgttcattca accagtcagc ttgggaagga ctagaaaaat gggggaaaga
    31441 tcaaggactt tcacgaggaa atgatggctt ccaatatttc caatcttcaa atgaatcaga
    31501 ttacatccca aatattgacc aagcattgaa tgctggattc aaaacgattt ttggtattgg
    31561 ttataaacta aaaccagcga tccaagaaca agcaacaaac aatacaggta cgaactttgt
    31621 tattatcgat gatgtcatcg atggattaga taacgttgta tctgcaactt tcaaagacaa
    31681 cgaagcttct tatcttgcag gtgttgcagc agcttatacg actgaaacaa acgtagtcgg
    31741 ctttatcggc ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg
    31801 tgttgatgca ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc aatatgctgg
    31861 tgacttctct gctccagata aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc
    31921 agatatcatc ttccatgctt caggcggtac aggtaacggt gtattccaag aagcaaaatc
    31981 attgaacgaa tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg accaatcaga
    32041 cgaaggcgaa tacacattaa acggcgaaaa gaaaaacttc acattgacat ctacactgaa
    32101 agcagttgga acagtagtag aagatttggc acaaaaatca gcggacggta aattccctgg
    32161 cggcgaacac actgtttatg gattgaaaga agacggtgtt ggtctaacag aaggacaact
    32221 ttctgacgaa gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt caggtgacgt
    32281 aaaagttcca gaaactcctg aagaaaacta aaaccatttc tatagccttg ttagacacat
    32341 gtttgacaag gctatggttt gattagaaca agaattgttc atttatccaa gctgccttgt
    32401 gacagtttgg ataagggaac aactccctga agtaatgttt gtttcaaaag aagaacgctc
    32461 actaagcgtg aatcttagtc aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa
    32521 aggtggttca agaaaatatt gtcatagaaa tgcagaatat taccaaagaa tttggaacat
    32581 tcaaggcaaa tgacaacatc aatttacaag taaaggcagg agaaattcat gcgttgcttg
    32641 gagaaaacgg tgctggcaaa tctacattga tgaacgtgct ttccggatta ttagagccga
    32701 catcagggaa aattttgatg cgtgggaaag aagtaaagat cacaagtccg accaaagcca
    32761 accaattagg gattgggatg gtccatcagc actttatgct tgttgatgcc tttactgtaa
    32821 cagaaaacat cgtgttggga agcgagccta gtcgcgcagg gatgcttgac cataaaaaag
    32881 cacgaaaaga gatccaaaaa gtttctgaac agtatggatt gtcagtcaac ccagatgctt
    32941 acgttcgtga tatttcagtt ggaatggaac aacgggtaga aattttaaaa acactttacc
    33001 gaggagcaga tgtactgatt tttgatgagc cgacagctgt attgacccct caagaaattg
    33061 atgaattaat cgtgatcatg aaggaattag tcaaagaagg caagtcgatc attttgatta
    33121 cgcataagtt agatgaaatc aaagcagtag ctgaccgctg tacagttatc cgccgtggaa
    33181 aaggaatcgg tacagtcaac gttaaagacg taacctcaca acaattagcc gatatgatgg
    33241 ttggaagaac ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt
    33301 tgtctattga aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga
    33361 acttagaggt tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga aacgggcagt
    33421 cggagttgat ccaagctttg actggtttgc gaaaggcaga aagcggacat atcaagctaa
    33481 aaggggaaga catcactaat aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg
    33541 tgccagaaga ccgtcataaa tacgggttag tcctagatat gacattatct gaaaatatcg
    33601 cactacaaac ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat tattcagtaa
    33661 tgaatgaaca tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg
    33721 ttcccgcaaa agctttatca ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg
    33781 accgagatcc tgatctgttg attgttgcaa atccaactcg tggactggat gtaggtgcga
    33841 tcgaatttat tcataaacgt ctgatcgaac aaagggacaa atacaaagca gtgttactga
    33901 tcagtttcga attagaagaa attttaaatg tttcggatcg tattgctgtt atccatgaag
    33961 gagaaattgt cggtatcgtt gatccgaaag aaacatctga aaatgaatta ggtctattga
    34021 tggcgggcta taccttagaa gaagcacgaa aagagttaga aaagggggct ggcgcacatg
    34081 aatgatcgca gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt tgttttagga
    34141 ttgatcttag gcgcgatatt gatggctgct tttggttttc agcccatcca aggttattct
    34201 tctatgctag atgcagccct tggttcgcaa agagggatag gtgaaacgtt aaggcaagca
    34261 acgccgttga tttttacagc attaggtttt tctgtagcta actcagctgg gtttttcaat
    34321 attgggcttt ctggacaagc actttgcggg tggatcgtca gtgtctgggt ggcacttgcg
    34381 tttccagatc tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg tgccttagct
    34441 ggtgcgatgg cagcggctgt tccaggttta cttcgagcat tttttggaac aagtgaagtg
    34501 atcgtaacaa tcatgatgaa ctatatctta ttatacgtaa gcacatttgc tttacatgaa
    34561 gtgatgcctg aaagctatcg ctcaagtttg gactcttcca atatgatcag tcaaaatgcc
    34621 tcactgcggt taccatggtt aacacaagct tttggcggct ctcgcgtaaa ctcaggatta
    34681 tttttagcaa tcatcgcgct tattttcgtt tggctgctga tgaagaaaac gactttagga
    34741 tttgaaatcc gttctgttgg attgaatcct tttgcttctg agtatgcagg aatgagtagt
    34801 aaacgtacga tcgttttgtc gatggtcatt tctggcgctt tggcagggct tggcggtgtg
    34861 gttgaaggat taggaacgta tcaaaacttc tttgttcaaa cgacttcgct atctattgga
    34921 tttgacggaa tggctgtttc cttgcttggt tctggatcag caatcggtat tcttttatca
    34981 gcgatcttgt tcagtatttt gaagatcggt ggacttggca tgcagacagg agcaggtgtt
    35041 ccgtttgaga tcgtcaatgt atcgattgct ttgattatct tctttgtcgg gatcaacttc
    35101 ctgattcgtt tcgtcatggc taaagtcttc caagggaaaa aacaagaaga gatcgttgcg
    35161 acaatagaat cgaaaccaac gaatcaaaac catgaagggg gcgaactata atgtcgacta
    35221 tcgaattaat tgccttgatc cttacttcta cactggttta ttcaacaccg ctaattctaa
    35281 cgtcactagg cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa
    35341 tcatggtcat gggtgctttt agttccatcg ttttcaactt gacgatgagt ccatcacttg
    35401 gttctatgac accatggatc gctattttag tgggtggcgt cgtaggtatc ctattttctc
    35461 tgattcacgc agtagcaaca gtcagtttgc gtgctgacca tattatcagc gggacagtaa
    35521 ttaatctgat ggcacctgct ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc
    35581 aaactgataa catcacgcag aatctggggt atttttcttt cccaggatta gcagatattc
    35641 cggtcattgg accaattttc tttaaaaata cattcttacc tgcttattgt gctattttga
    35701 ttgctgtcct agcttgggtg atcatcttca agacgaaatt cggcttgcgc ctccgttcag
    35761 ttggtgaaaa tcctcaagct gccgacacat taggtatcaa tgtatatgct atgcgttatt
    35821 caggcgtact gatttctggc ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga
    35881 tttccggtaa cttttcagct ggtacgatcg ttggacaagg gttcatctcc atggctgcta
    35941 tgatctttgg taagtggaat cctttagggg caatgggtgc ctcattgttc tttggttttg
    36001 ctcaaagctt gagtatcatt ggcgcacaat taccagttat ttcttcgatt ccgccagtat
    36061 tgctgcaaat tgctccatat ttgctgacaa tcattgtgct ggttgttttc ttagggaaag
    36121 cttctggtcc aaaagcaaac gggaagaact atatcaaatc aaaataatct tttagcgaat
    36181 aaaaagggtg ggttacggga agtgtttttc gtctcgccct ttttgtttta aaggcaataa
    36241 aaataaaaag aaagaaggaa taatcatgtt taaacgcgta catctgatcg tcatggattc
    36301 agtaggaatt ggagaagcac cagatgccga taagttcggt gatgttggtt ctgatacatt
    36361 aggacacatt gcgaaagaag caggattgaa cgttccacat ttagagcagc ttggtttagg
    36421 aacgatccgt cctcttgaag gtgtggaatc agtgaaagat catcaaggct atgcgacgaa
    36481 attagaagaa gtttctgtag ggaaagatac gatgacaggc cactgggaaa tcatgggatt
    36541 gaatatcaaa actccatttc gtgtgtttcc taatggattc cctgatgatc tgatcaaaca
    36601 aatcgaagaa ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt
    36661 tattgacgat tacggtgaac accaaatgaa aacaggcgat ttaattgtat atacttctgc
    36721 cgatcctgtc cttcagatcg cggcacatga agaaatcatt cctttagaag aattatatcg
    36781 gatttgccaa ttcgttcgtg atataacaaa agatgatccg tatatgattg gccgaatcat
    36841 tgcacgtccg tacgtgggtg aaccaggaga cttcacacga acaagcaatc gccatgatta
    36901 tgcgttgaat ccattcggac acacagtcct tgattctttg aaagaagcag gaaaggacgt
    36961 gattgctgtt ggaaagatca acgatatttt caacggacaa ggaatcactg aatctgtccg
    37021 cacgaaaagc aatatggatg gcgttgacca gctgctgaaa gttatgaaaa acgatttttc
    37081 aggaatcagc tttaccaatt tagtagattt tgatgcatta tacggtcatc gtcgggatgt
    37141 agtcggctat gcgcatgcga ttgaagaatt tgacttacga attccagaat tgcttgaagc
    37201 gatggaagaa gacgacttgc taatgattac agccgatcac ggaaacgatc cgacgtttac
    37261 tggtaccgat catacgcgag aatatgtacc gttattggtt tacagtaaaa aaatgaaaga
    37321 acaaggggca ttgcctcaag gatattattc agatatttca gcaacgattg ctgagaactt
    37381 tagtgtttct tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat aatcttttta
    37441 aataggagaa agcaaatgac caagttaagc gagatgttac aagaaacgac ccagtttatc
    37501 cttgataaag gagtaaaaga aattgatttt ggtttgattc taggttcagg attaggtgaa
    37561 ttagcagatg aaatcgaaga tgctgtaatg atttcttatg atgagatccc gttctttcct
    37621 gtttcgacag tagttgggca tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa
    37681 gtgttagcca tgcaagggag attccactac tatgaaggtc attccatgca aacagtgacc
    37741 tatccagtac gtgtgatggc cggactaggg gcccattcag tcattgtgac taatgcatgt
    37801 ggtggtgtca acgaatcatt tgtaccagga gatttgatgc tgattaccga tcatatcaat
    37861 tttactggtg aaaatccttt gatcggtccg aatgaagatg caatgggccc tagatttcca
    37921 gatatgagtc aagcttatac gcctgactat caagcaacag caaaagcagc agcagaaaaa
    37981 ctaggtttgg atcttaaaga aggcgtatat atgggctatt ctggaccaac ttatgaaacg
    38041 cctgcggaga tccgaatgtc tcggacaata ggagccgatg cagtagggat gtcgactgtt
    38101 ccagaagtca tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc ttgtattacc
    38161 aacttagcag caggtatgca agcgaatctg agccatgaag aagttgtaga aacaactcag
    38221 agagtcaaac aatcttttaa agcattggtt aaagaaacat tgacattatt gtaataggag
    38281 gaacaataaa atgagtgtgc atattgaagc gaaagaaggc gagatcgccg ataagatttt
    38341 acttccggga gatcctttgc gagctaaata tattgcggag acttttttgg aagatccagt
    38401 ttgttacaat caggtacgcg gtatgttagg ctttaccggc aaatataaag gagaacgtat
    38461 ttctgttcaa ggaacaggaa tgggaatgcc atcagccacc atctatgccc atgaattgat
    38521 ccagtcgtat ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga
    38581 cgtccatgtt cgtgatttag tactagcaca aggtgcagcg accagttctt cgatgatcga
    38641 aaaaaatttc caagcctttc attttcctcc aatcagtgat ttcaatctat tactgaaagc
    38701 atatgagata gctaaagaaa agggctatac ggttcatgta ggaaatgttc tttcggaaga
    38761 ttctttctac aaagatgatt tgacagagac gttccaatta gctgaacttg gcgttttagg
    38821 agtggaaatg gaagcagcgg ctctttatta cttaggcgcg aaataccatg tgcagacatt
    38881 aagtttgatg accgttagcg accatttgat caccggtgaa gaaacaacag ctgcagaacg
    38941 tcaatctacg ttcaatgaaa tgatcgaagt aggattggaa acagcgatag cagaataaga
    39001 aggaaatgaa aaagctgccc tgcattaagt aataagaaca tccaagcaaa aatagctcct
    39061 catgtttttg acttggctgg acttattatt gcaaatg
//
LOCUS       DACEOE010000008        35438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEOE010000008 DACEOE010000000
VERSION     DACEOE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.008"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1152
                     /locus_tag="IW774_000377"
     CDS             <1..1152
                     /locus_tag="IW774_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152757.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IW774_000378"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IW774_000378"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1152758.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IW774_000379"
     CDS             2728..3003
                     /locus_tag="IW774_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1152759.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IW774_000380"
     CDS             3009..3776
                     /locus_tag="IW774_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1152760.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRIYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IW774_000381"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IW774_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1152761.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IW774_000382"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IW774_000382"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1152762.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGRNKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IW774_000383"
     CDS             7920..8222
                     /locus_tag="IW774_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1152763.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IW774_000384"
     CDS             8225..9388
                     /locus_tag="IW774_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1152764.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IW774_000385"
     CDS             9414..12842
                     /locus_tag="IW774_000385"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1152765.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IW774_000386"
     CDS             12863..13987
                     /locus_tag="IW774_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152766.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IW774_000387"
     CDS             13977..14414
                     /locus_tag="IW774_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1152767.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IW774_000388"
     CDS             14398..14736
                     /locus_tag="IW774_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1152768.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IW774_000389"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IW774_000389"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1152769.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IW774_000390"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IW774_000390"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1152770.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IW774_000391"
     CDS             15893..16936
                     /locus_tag="IW774_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1152771.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IW774_000392"
     CDS             17095..17823
                     /locus_tag="IW774_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1152772.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IW774_000393"
     CDS             17939..18184
                     /locus_tag="IW774_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1152773.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IW774_000394"
     CDS             18181..18399
                     /locus_tag="IW774_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1152774.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IW774_000395"
     CDS             18535..19074
                     /locus_tag="IW774_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1152775.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IW774_000396"
     CDS             19067..21340
                     /locus_tag="IW774_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1152776.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IW774_000397"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IW774_000397"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1152777.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IW774_000398"
     CDS             complement(22513..22767)
                     /locus_tag="IW774_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1152778.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IW774_000399"
     CDS             complement(23167..23436)
                     /locus_tag="IW774_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152779.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IW774_000400"
     CDS             complement(23906..24361)
                     /locus_tag="IW774_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1152780.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IW774_000401"
     CDS             complement(24499..26427)
                     /locus_tag="IW774_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1152781.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IW774_000402"
     CDS             complement(26624..27457)
                     /locus_tag="IW774_000402"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1152782.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IW774_000403"
     CDS             complement(27649..28353)
                     /locus_tag="IW774_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1152783.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IW774_000404"
     CDS             complement(28376..29002)
                     /locus_tag="IW774_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1152784.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IW774_000405"
     CDS             29328..31703
                     /locus_tag="IW774_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1152785.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IW774_000406"
     CDS             complement(31727..32218)
                     /locus_tag="IW774_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1152786.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IW774_000407"
     CDS             32405..33325
                     /locus_tag="IW774_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1152787.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IW774_000408"
     CDS             33479..33823
                     /locus_tag="IW774_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1152788.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IW774_000409"
     CDS             complement(34013..34513)
                     /locus_tag="IW774_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1152789.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IW774_000410"
     tRNA            35041..35124
                     /locus_tag="IW774_000410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
BASE COUNT        11777 a         6169 c         7295 g        10197 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tatacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtaga
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaa
//
LOCUS       DACEOE010000009        32867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEOE010000009 DACEOE010000000
VERSION     DACEOE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.009"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(31..879)
                     /locus_tag="IW774_000411"
     CDS             complement(31..879)
                     /locus_tag="IW774_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1152790.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1103..3043)
                     /gene="thrS"
                     /locus_tag="IW774_000412"
     CDS             complement(1103..3043)
                     /gene="thrS"
                     /locus_tag="IW774_000412"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1152791.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3380..3559)
                     /locus_tag="IW774_000413"
     CDS             complement(3380..3559)
                     /locus_tag="IW774_000413"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1152792.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3606..5429)
                     /gene="cadA"
                     /locus_tag="IW774_000414"
     CDS             complement(3606..5429)
                     /gene="cadA"
                     /locus_tag="IW774_000414"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1152793.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5707..7986
                     /locus_tag="IW774_000415"
     CDS             5707..7986
                     /locus_tag="IW774_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1152794.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8079..8726)
                     /locus_tag="IW774_000416"
     CDS             complement(8079..8726)
                     /locus_tag="IW774_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152795.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8905..9624)
                     /locus_tag="IW774_000417"
     CDS             complement(8905..9624)
                     /locus_tag="IW774_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1152796.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9755..10906)
                     /locus_tag="IW774_000418"
     CDS             complement(9755..10906)
                     /locus_tag="IW774_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1152797.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11123..11863)
                     /locus_tag="IW774_000419"
     CDS             complement(11123..11863)
                     /locus_tag="IW774_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1152798.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11867..12190)
                     /gene="rsfS"
                     /locus_tag="IW774_000420"
     CDS             complement(11867..12190)
                     /gene="rsfS"
                     /locus_tag="IW774_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1152799.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12247..12840)
                     /locus_tag="IW774_000421"
     CDS             complement(12247..12840)
                     /locus_tag="IW774_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1152800.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12824..13471)
                     /locus_tag="IW774_000422"
     CDS             complement(12824..13471)
                     /locus_tag="IW774_000422"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1152801.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13484..13795)
                     /gene="yhbY"
                     /locus_tag="IW774_000423"
     CDS             complement(13484..13795)
                     /gene="yhbY"
                     /locus_tag="IW774_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1152802.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13811..14920)
                     /gene="yqeH"
                     /locus_tag="IW774_000424"
     CDS             complement(13811..14920)
                     /gene="yqeH"
                     /locus_tag="IW774_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1152803.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14917..15444)
                     /locus_tag="IW774_000425"
     CDS             complement(14917..15444)
                     /locus_tag="IW774_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1152804.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15624..16409)
                     /locus_tag="IW774_000426"
     CDS             complement(15624..16409)
                     /locus_tag="IW774_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1152805.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16402..17271)
                     /locus_tag="IW774_000427"
     CDS             complement(16402..17271)
                     /locus_tag="IW774_000427"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1152806.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17272..18651)
                     /locus_tag="IW774_000428"
     CDS             complement(17272..18651)
                     /locus_tag="IW774_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1152807.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18659..19078)
                     /gene="fabZ"
                     /locus_tag="IW774_000429"
     CDS             complement(18659..19078)
                     /gene="fabZ"
                     /locus_tag="IW774_000429"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1152808.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19103..19579)
                     /gene="accB"
                     /locus_tag="IW774_000430"
     CDS             complement(19103..19579)
                     /gene="accB"
                     /locus_tag="IW774_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1152809.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19582..20817)
                     /gene="fabF"
                     /locus_tag="IW774_000431"
     CDS             complement(19582..20817)
                     /gene="fabF"
                     /locus_tag="IW774_000431"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1152810.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20836..21573)
                     /gene="fabG"
                     /locus_tag="IW774_000432"
     CDS             complement(20836..21573)
                     /gene="fabG"
                     /locus_tag="IW774_000432"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1152811.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21585..22502)
                     /gene="fabD"
                     /locus_tag="IW774_000433"
     CDS             complement(21585..22502)
                     /gene="fabD"
                     /locus_tag="IW774_000433"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1152812.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22609..22833)
                     /locus_tag="IW774_000434"
     CDS             complement(22609..22833)
                     /locus_tag="IW774_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1152813.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22886..23848)
                     /locus_tag="IW774_000435"
     CDS             complement(22886..23848)
                     /locus_tag="IW774_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1152814.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23858..24298)
                     /locus_tag="IW774_000436"
     CDS             complement(23858..24298)
                     /locus_tag="IW774_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1152815.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24604..25569
                     /locus_tag="IW774_000437"
     CDS             24604..25569
                     /locus_tag="IW774_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1152816.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25626..25856)
                     /locus_tag="IW774_000438"
     CDS             complement(25626..25856)
                     /locus_tag="IW774_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1152817.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25942..26829)
                     /locus_tag="IW774_000439"
     CDS             complement(25942..26829)
                     /locus_tag="IW774_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1152818.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26918..28141)
                     /locus_tag="IW774_000440"
     CDS             complement(26918..28141)
                     /locus_tag="IW774_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1152819.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28158..29237)
                     /locus_tag="IW774_000441"
     CDS             complement(28158..29237)
                     /locus_tag="IW774_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1152820.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29368..31095)
                     /gene="ptsP"
                     /locus_tag="IW774_000442"
     CDS             complement(29368..31095)
                     /gene="ptsP"
                     /locus_tag="IW774_000442"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1152821.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31095..31361)
                     /locus_tag="IW774_000443"
     CDS             complement(31095..31361)
                     /locus_tag="IW774_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1152822.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31840..>32867
                     /locus_tag="IW774_000444"
     CDS             31840..>32867
                     /locus_tag="IW774_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1152823.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKK"
BASE COUNT         9140 a         7164 c         5702 g        10861 t
ORIGIN      
        1 aaaaaactta tataaaaaat agtaaggttt ttatttcgaa tccataaaat attcttggat
       61 tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca tatagaaata
      121 aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg cacttcgatt
      181 cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc taagcgggtt
      241 atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca gctcggctaa
      301 tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt tctttttctc
      361 attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga ttggcttacc
      421 gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc caaccccata
      481 taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg tttcttcttc
      541 tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat
      601 agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc cagtctgtag
      661 ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt ttttggaaaa
      721 acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag ctgtcattgt
      781 agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg ctgaagaata
      841 atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca cccttttaat
      901 gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac tggataaaaa
      961 acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag attctattga
     1021 gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc tatctactac
     1081 ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact tctgcttcca
     1141 tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca taacgacgta
     1201 cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa gggatctttt
     1261 gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca acttctacac
     1321 gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca tgtgcatcaa
     1381 cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt
     1441 atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg cggtggataa
     1501 ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca aagcgctctg
     1561 gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga gctgttttga
     1621 cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta
     1681 gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac attgcgtcat
     1741 catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa cgataatctg
     1801 taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta aattcatctt
     1861 tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct cgtacacgtt
     1921 gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat tcagcgatac
     1981 gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg tgatgagggc
     2041 agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc gggaacatgt
     2101 cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct cccattattg
     2161 gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa cgttcgatcg
     2221 tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct acttctgggg
     2281 aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct tttgcttctt
     2341 cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac
     2401 gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact gataataatt
     2461 taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa tcgatgaatt
     2521 cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc agttctactt
     2581 tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca cggcgttcga
     2641 tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc aaatcttctg
     2701 cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc
     2761 caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc aaatgagctg
     2821 ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc tcaatacttg
     2881 catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta ccagctaaag
     2941 ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt gttccagact
     3001 caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt tcctccttga
     3061 aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc atctcttgaa
     3121 aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc
     3181 tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc
     3241 tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga agactctcaa
     3301 attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat aaatagaaaa
     3361 atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat gttccttttt
     3421 tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag
     3481 cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct tctactaatt
     3541 cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt ttcaactcga
     3601 ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg atcttttttt
     3661 gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac agctccgaat
     3721 aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat aaagatgcca
     3781 atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac tgcttttgct
     3841 acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc agctgtagaa
     3901 ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc gtcattcacg
     3961 ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa aatggtcagt
     4021 ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt
     4081 tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc aagttttctt
     4141 agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag gatcgttcct
     4201 gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat ttcttcagct
     4261 gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc ttggattcct
     4321 tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt cacgttgttc
     4381 actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc atacagaagt
     4441 agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa tatccctttg
     4501 gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt
     4561 gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc aactaaagca
     4621 atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc agcaaatcgt
     4681 aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat caacgtgaaa
     4741 gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt tgattcttcg
     4801 actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc gatcatttgg
     4861 atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt cttggtttcc
     4921 gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt ggtcactcga
     4981 ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat atttatttca
     5041 tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct tggggtgtta
     5101 tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc tagactatct
     5161 ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac ggctaatgtt
     5221 gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg taacgtcttg
     5281 atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac aatcgcaaat
     5341 gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc aataagcaaa
     5401 gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg tgacttagag
     5461 aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt gactagcact
     5521 ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc caaaattcat
     5581 cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca gttaaaaagt
     5641 accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg aaggagcaaa
     5701 catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat cgaactaatc
     5761 cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga aatgaatcat
     5821 tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc tttttatgaa
     5881 tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca tactcttgaa
     5941 gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta ttttgcccgt
     6001 atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc ttctttaaga
     6061 gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa tctttattat
     6121 gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga tgtcgatctg
     6181 cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt agatacgtcg
     6241 gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag caaccatatg
     6301 aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga cacaacaagt
     6361 aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt gcttcaacgt
     6421 attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt tcttctattt
     6481 tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt aggtgaaagc
     6541 ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga gttttcttta
     6601 ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc gatacaagtg
     6661 atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg catcacaaac
     6721 tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc aaaggaatcg
     6781 attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag actttctcta
     6841 ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac acgtcagcaa
     6901 tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga tccaaatctt
     6961 gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca aatcgtacgt
     7021 aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa tataacgaaa
     7081 caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca taaaatcagc
     7141 gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca aagagaattg
     7201 cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc agtgacggtt
     7261 acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc atcacaagtg
     7321 gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga tctaaatcaa
     7381 aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt ggaccgtgaa
     7441 gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac tgattttgct
     7501 aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg agaccttcct
     7561 ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga taccattcga
     7621 acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac acaagaagaa
     7681 tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca gctgatttta
     7741 aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt agcaaaaggt
     7801 ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc ttctcagcaa
     7861 gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac cctttttacc
     7921 tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga gaaaaaagta
     7981 ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt atttacaata
     8041 ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata taaatccaaa
     8101 tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc ttccatgttt
     8161 tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt gtcgatcaat
     8221 cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct gattggatag
     8281 ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc gatcacttta
     8341 ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg acgatgtttc
     8401 aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc aaataaccga
     8461 aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct ataaatcagc
     8521 attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt accgacatag
     8581 tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa ttttgaacaa
     8641 tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat acgttcaaaa
     8701 ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca tacttcatat
     8761 catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa agaaaataaa
     8821 tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga aaaagactga
     8881 atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga acacatgctg
     8941 tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt gatcttctgg
     9001 agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa aatcagtgat
     9061 tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac cgtaaacgat
     9121 gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca ttaggtattc
     9181 aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt caaacatgta
     9241 agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg cccgtacgtc
     9301 tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg agccgcttgg
     9361 tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt ttgctttttc
     9421 gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat caatgacgaa
     9481 acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg cgtagatttc
     9541 aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt tctttgctac
     9601 gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat agtttcataa
     9661 cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc tttttttctt
     9721 tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg atttgcccga
     9781 aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta actaacagtt
     9841 ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct tttttttctt
     9901 ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt ttattttgtt
     9961 ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat cgttggatcc
    10021 gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa ctatcagcag
    10081 ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag atatctgtca
    10141 gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa gattcccaag
    10201 tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt ccagctattc
    10261 tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc tgatctgtga
    10321 tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc atagggtgga
    10381 catacttggc attttccttt gcataactta atcccaagat atgatttggc gaagaaaaat
    10441 caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc ggataactat
    10501 acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc tcttggacaa
    10561 agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa cacaaactgt
    10621 cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca gctgactgaa
    10681 ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa gcttcattcg
    10741 cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc ccgctcatta
    10801 cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga tagcgatgtc
    10861 cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc atcctccttc
    10921 atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc ggaaaaaata
    10981 cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa gctgcactgc
    11041 aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat tcttttcttg
    11101 ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag cgctttgatt
    11161 tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa aagccgctgt
    11221 tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt agttcatctt
    11281 cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga aaatgttcga
    11341 tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta tccccttgat
    11401 aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt acgtcaaata
    11461 aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc tgtacctctt
    11521 gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga tattgtccga
    11581 tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct tcttcatatg
    11641 ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc actttatatc
    11701 catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc aaaatatttt
    11761 ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa agtgtttcgt
    11821 ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa tccaaccatt
    11881 cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag aaatttcgtt
    11941 ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc cattttccgc
    12001 cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta tcaatgattt
    12061 cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg aaataatctg
    12121 ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt tttgaatctg
    12181 ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc tcctaatatg
    12241 agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg gataaatctg
    12301 ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta cagcttcgtc
    12361 tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc gacctggttc
    12421 aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag ccccagtcgt
    12481 atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc gctctacaaa
    12541 cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact ccggatcaaa
    12601 accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg cataatcatg
    12661 tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg caagagccac
    12721 ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc tcatctgtat
    12781 tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt ccatattcat
    12841 acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag cggatagaac
    12901 agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca ggtacatcta
    12961 cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata cccacaaagt
    13021 tgaccataga agtcagctca tcgattttat accattttgg caaatattct accatatccc
    13081 caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc ttcatagtat
    13141 cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc aaaaaaggat
    13201 tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc gtctgtttct
    13261 catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga cctaaattcc
    13321 ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat gttccgccca
    13381 agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga aataattgtg
    13441 aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt gcttttactt
    13501 cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca ataacacgtc
    13561 caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct gcttcttctg
    13621 caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt tctaaagctt
    13681 cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga aaaatcggct
    13741 gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa ctcataattt
    13801 tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt ggcgcccagc
    13861 ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa acgatgtctg
    13921 ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg acttcatcag
    13981 gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct gctttttcca
    14041 gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa gcacgattgc
    14101 cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct tcattcagct
    14161 gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct tttttaccta
    14221 agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa aaatgcccgt
    14281 catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa gttgtgatta
    14341 catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat tttccgacat
    14401 ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt ttttctaata
    14461 gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact ggacgaattc
    14521 ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc aatgatttag
    14581 gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca aaacggtgta
    14641 gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg atcaatgcat
    14701 ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat gagacatctt
    14761 gggtctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc ccggaagcta
    14821 aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg tttgtttgta
    14881 taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac ctcgccagcc
    14941 catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa aacgattaaa
    15001 ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac gtatccctgc
    15061 acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta tctcatcatc
    15121 tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt tcattgctcg
    15181 tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg aattattgtt
    15241 tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag taagcaactc
    15301 ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg tcaatactgc
    15361 tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt ctaccatcca
    15421 tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga aggagcacat
    15481 aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac ctctatactc
    15541 gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga ttgatcacag
    15601 aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg tttttccagt
    15661 aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc ttttttcata
    15721 atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg aataactcga
    15781 tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc agctgcttct
    15841 gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga aagcacagca
    15901 taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag ggcaccgcca
    15961 cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga catttcgagc
    16021 agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc gcaatatgca
    16081 cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg tttcatcagt
    16141 cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg gatattttcc
    16201 tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc taatcgagcg
    16261 attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca ttcctcgaaa
    16321 gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc tcttgctaac
    16381 tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg tatgcaattc
    16441 aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat cgacaaaacc
    16501 atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct tgatcgtctg
    16561 ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta gaataatatc
    16621 gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta aaacagttag
    16681 ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg ccatctgcat
    16741 tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca agacgactgg
    16801 aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga caatcgttcc
    16861 catacttccc atgataaaat taggatccat cactccaata atgaagggaa tcccctgaat
    16921 ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca tttttttaat
    16981 tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg tatcccattc
    17041 ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga ttcgaaaagc
    17101 gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa ggatatgttt
    17161 gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag caggtttgct
    17221 ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca tctatgcgcc
    17281 tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa atgctgtgct
    17341 gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt ctgcgttcgt
    17401 gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta atgcttcaaa
    17461 acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg gcgggatcgt
    17521 atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg gaagcagtaa
    17581 gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg cattaatccg
    17641 gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca aaggctcacc
    17701 ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt ctgttactgg
    17761 atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt cttcatccat
    17821 cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc gaacagcagc
    17881 gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt cttccagtac
    17941 tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga catgaccaaa
    18001 tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt tttccaaata
    18061 catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa aatgctgtgg
    18121 gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg cagctgcttt
    18181 cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta ctgtagataa
    18241 tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt gacgcgcatt
    18301 gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg tgatattgca
    18361 ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc ctggatgaat
    18421 tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca gatatgaatc
    18481 aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat gaagtgcttc
    18541 ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc atgcacgaat
    18601 gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca tatttcacct
    18661 atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact ttagcgattc
    18721 ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg tataaggtat
    18781 ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa taagccgtct
    18841 ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc attgcttcaa
    18901 caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa aagggctcat
    18961 tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct tccactcgat
    19021 caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt atgttcattg
    19081 ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc tgtccatatt
    19141 cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc tcactagtta
    19201 tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg gcaacttgat
    19261 cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg attcccacga
    19321 ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc attatttctg
    19381 gagcagttgt tgcttgttca ggtatgacat tgactggctc cgaaactggc aacgtttcgt
    19441 ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac aattcaaatt
    19501 gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg actaattctc
    19561 tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa ccgcattatg
    19621 tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt tgctttcgtt
    19681 tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg taggtgggat
    19741 aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg ctgctcccaa
    19801 taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat tttcacctaa
    19861 tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg ttccgtgagc
    19921 attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa gcttcattgc
    19981 tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt cacagtttgc
    20041 accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat gttctaaaga
    20101 ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat tttcgtcaaa
    20161 cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag ctgcaaatcc
    20221 agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga catcagaata
    20281 gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag cacaagctgt
    20341 tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc cagctgccat
    20401 gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt tttcatgtag
    20461 acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga tgatgacgcc
    20521 aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg cttctaaagc
    20581 agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt taataaaata
    20641 tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt ctgaagcatc
    20701 aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca aaaagctgtc
    20761 tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc gattcatttg
    20821 tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga tgacttgtcc
    20881 ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt ctacttgtcc
    20941 gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg ctaaaacttc
    21001 cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc ctctagttgc
    21061 agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag ctgcataatt
    21121 tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga tggccccttc
    21181 tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc caatcaaatt
    21241 gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt tgtcatttgt
    21301 gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg cttctttgat
    21361 catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc attttacacc
    21421 gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat tcaaaatgat
    21481 atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc ttgtactacc
    21541 tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag tattgctatt
    21601 gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt atcgattttt
    21661 ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc gatgccgatt
    21721 gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg ctgttcaagt
    21781 agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat gataggaaaa
    21841 gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc tgccggttct
    21901 aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt gaaccctttt
    21961 tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat cacgatctgc
    22021 tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc atgacagctt
    22081 tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct tccagctggg
    22141 gcagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc agtgaaggat
    22201 agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa agctgcatct
    22261 gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt tagaatagct
    22321 ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag ttcagccata
    22381 tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc ttcttcatat
    22441 aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa tgctgttttc
    22501 atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa aaaggccgag
    22561 acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt atgcagtttg
    22621 tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg tttcgatttt
    22681 cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc tgtcagcttc
    22741 taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc ctaattcttc
    22801 tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg atattgtttg
    22861 ctttttattt ttataattta acagtctaca actccatgag aatacttccc catgttaaac
    22921 ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc tttccactag
    22981 aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca tatttatcca
    23041 tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc tcaataattc
    23101 tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg acttcttgga
    23161 tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc atttgcaaat
    23221 aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg tagccagcgg
    23281 tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat tttttctgtg
    23341 gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca gtcgtacgat
    23401 cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc ttagttgagc
    23461 cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag cacgcagcac
    23521 tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt gctgctacag
    23581 atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa tctttagggt
    23641 ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca gaagtctcct
    23701 gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat tcatcagagg
    23761 tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg acgctggctg
    23821 ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct tgcaagaagt
    23881 cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc attccgtcta
    23941 agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt aactttcctt
    24001 ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata cgttcgacat
    24061 agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta attgatagtt
    24121 ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg atcgtgtgca
    24181 tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct tcgattgtca
    24241 aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct ggttccatgt
    24301 tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact atatcttaac
    24361 ttttttcaaa atgcaacctt aaaagatcat ttttttacta tatgagcttt ttttaagatt
    24421 ttactcattt aaaacaactg ataaatcagc atttataatt tcttttatta gaaaaatcat
    24481 ctatctcaca tattttgatt atcaaagtat tttctgtgaa cagaactata gaattcccta
    24541 aaacttgtcc attatttcaa aatttgttat agtcttttta ttcaaagcgt aaaaggagat
    24601 cctatgttaa aagaaaaaat gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa
    24661 gaccgagatg gtaaggtcat ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc
    24721 agctattttc atcagaataa acagcattca aaaaaagtat tacgtatgat tcatcatata
    24781 acagattttg atttcgtcgt agtagacacg gaattagatg ctttattgct cgagtgtcaa
    24841 ttgatccaac attaccgtcc tttttataac cgacaaatga attatttcag taattataat
    24901 tatgtacata ttaccaataa gggatttgta ttaacagata ctcctacggc tcgaacatac
    24961 gggccttttc gtctctataa aaaaatgccg tctattttgc gtataatgga agaaacttat
    25021 caaatgcctt ggttgtctga aattagtttg ttagctttgc gagtccagct tcctgaccta
    25081 caggaaatga cttttgaaca gaaaaaaaag gaactccaag ggctgtttca aggccgaaat
    25141 aaaaagctac tgacttactt gaaaaaaaga caacaacatt tcatctatca gttgaatttc
    25201 gaaaaagccg gtatgcttca aaaagatatc gaattagtta cttattttat tagacggatc
    25261 caggaacaaa agcagttttt aagaacacca tcgctcacct tcagcatgcc attagcagca
    25321 gatgagagtc agaaaaagca ctacctcgtt tgttatggtc aacttgcaga aacaatgatt
    25381 acctctggtg acgtttctcc tgacttctat tatgaaaaga aagaagctca tttatctttg
    25441 aaacgtcaat tgtcaaaaga agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa
    25501 aaattagaaa aagaacagtt tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa
    25561 ctaaattagt tgcttctcgg cttcttttag gcatcttcaa cttcaaatgt caattatcct
    25621 aaaatttatt ggattaagtc atagatttcc atcgctacga tatcgatatt atcaaattcg
    25681 tattgctgat gagtatctgt ttcagttaat tcaaatgtat cggtcgcttt atcataagtt
    25741 actgtacact tctctacgcc ttctttttca aaacggcgaa cttgcagttc attatctgct
    25801 gactcgacca tagcctccag tcgttttatg attgcgacta attgtgaatg tgtcatcgga
    25861 ggtcctcctt ttcttatttc tgttatattg tatcaaaagc atagcctact tgcattgatt
    25921 ttctctaaaa aaactcgttt tttatgattt tttttctggt atttttcctt caggccacca
    25981 aagtttgatc aaagcttggg ttccgtcatt ctcaaaacct ttatctgcca actcttcata
    26041 tagtttcaat gcttgggtag ttgctggcaa tacaagatcc attttttctg cttcttctaa
    26101 tgcaatcttt aaatctttga taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt
    26161 taaaatacga ggagaataat tgcttaaaga ccagttagct gcacttccac ctgaaagcgt
    26221 ctcaatcact ttctttaaat caagtccgtt tttttgtgca tagacaagca tttccgtcat
    26281 tcctgtcatc gtacctgcaa tcatgatctg attagccatt tttgtatgct gacctttccc
    26341 agcagaacca tgcaatgtaa atgtcgtacc aaattctttg aataaaggca gtacttgatc
    26401 ataaacggct tcttctccac cgaccatgat cgtcagtgta ccattctttg ctcctaagtc
    26461 accaccggat accggtgcat ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat
    26521 tttttctgcc aggcttggtg tacttgtagt caaatcaact aaaatatgtc ctgagatatc
    26581 tgcagaaaaa atccccgact gtccatagta gatttcttct acatctttgg ggtaacctac
    26641 cattgtaaag agaatattac tttgttctgc taccgctttt ggtgtatctg cccagatagc
    26701 tccttctgtt actaaaggat cagcttttgc ttttgtccga ttatagacag ctacttcgtg
    26761 tcctgcttct agtagatggc gtgcgacagc acttcccatg acgcctgttc cgataaaacc
    26821 aagtttcatt ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc
    26881 tagaaacaag gattttttca taaaaaatgt ttgaatttta tcttttaaat gaatttaatt
    26941 ttattttgat tcgttcaatc gagtcttctt cttcttttct tcgcatctct tcttcatgat
    27001 aaacaaccat gtcttgataa aattcgatca tagcttcgcc gaaatgtgta gatgaaatat
    27061 cataaagttt agcttctaaa acttttggat ctttaggaac ttcactggaa atataatcaa
    27121 taagtgctgg tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa ggatgatcga
    27181 acaatcggtt caaataagca tttccttcca cgacacaagg tgttccggct gccatcgctt
    27241 ctgtataagt aagcccttgt gtttctgaag tagaagcact gacaaaataa tctgccgctt
    27301 tataataaag tgctacatca ttgttgtcca cttctcctgt gaactgcaca aattcttcta
    27361 actgcagttc tgagatcaat tcctctaact cttctacata aggtcctttt cctacgataa
    27421 ctaatctggc gttaggataa gcggagatga cctgaggaaa cccattaatc aaggcttgga
    27481 tatttttctc ataagaaatc ctgcttaaag aaagaatcat caaatggttt tcctgaagac
    27541 ctagtgactc ccttaatttg ctgatatcct gctgagtgat atcagaacgt ttgaattttt
    27601 caatgtcgat accagtagga atcacgcgaa gcggtgctat tacaccatag ctgcgtaatg
    27661 tatcaataac acgttcactt ggacaaacta cacctgtcgt atgatttgca aataatctag
    27721 acaagtattt tacatgggat tgcctgatca cttttccttt accgatataa tgaaggtagt
    27781 cttcatacat cgtatggtaa gtatggatca gaggtatttt caacttctta gctaccatct
    27841 tcccaagtat ccctgctccg aattctgtat gggtatgaat aagatctagt tctagttctt
    27901 tggcaaccaa ataagcatac cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa
    27961 taaatggtac acttggcatt cgaatcacat ctttttccaa cttatccgca ttaggatcgg
    28021 tcgttgtaaa aatatagact tcatgcccat gtctttctaa ttcttgtttt aatgttttga
    28081 tcgatgtagc aactccgctg acttgcggaa agtaggtatc ggtaaaaaag ccgattttca
    28141 tgtttttcgc ctccaaacta gtccttgacc tgatttactt tttttaattt gctagagact
    28201 ttttcattca gtacactttc gtatacattt ttaagttctt caccgatttt attgatactt
    28261 cgctcttttg ctgtttgata accggcttgt gtcgtactcg gtagttcttg attggtaatc
    28321 tgttggatca gtcggataaa ttcttcgttt gtcttcccca tatagcagtt cttgctatct
    28381 tctaaccagc cattatacac tggaatatct cgaacaagta cgttttgttt gcttgctaaa
    28441 gcttctaaaa caacgatccc ttctgtctct tcataagaag ggaaaaagaa tagatctgca
    28501 cctgaatacg caccttctat gatctctcca cgaatgtaac caggaaacag gacattttca
    28561 ggatgatctt cttttacaat tttccggatg ttccgaggaa tcgagtacat gggaacatga
    28621 ccaaaccaaa taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt
    28681 cctttacgtt cgaaaaacaa accgacacaa atgattactt tttgatccgg agacagtttg
    28741 aaatattctc tgaacttttg ttctttcatt ggatcagggt gatatttctc taagtcgatc
    28801 ccattggaaa tagcttgaat cggcacacgg ataccatagc tttccaacaa ctgttttgaa
    28861 taaggtgttg gcgtgatcag atagtccgct ttttggtaca atcctaccag atattttttt
    28921 acgataggag aaagctggtt cgatccgata aaggaatttc ggaaatcttc ctctgtagaa
    28981 tgcgcatgat agacaacttt ttttccttct cttctggctt tattgaccat attatgacta
    29041 ttgattccgt atgtattgat gtgcaggatg tcataatctt ctttttcatc tagtgtataa
    29101 gagataccga cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc gattccagat
    29161 tttgcaagca tcttttcact ttcaaaatat aataatacct tcaccttacg tcccccttca
    29221 cttatatagt ataacatagc ctacaaactg tcactcaaac ttaagcccta tttttgtcta
    29281 ttcttactga caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta gctcttttac
    29341 aagccacacc agcttttgat acgttgttta ttttacatat tcatgaacaa gttcaacgac
    29401 ttcttccatc gtatcgcatt ctttcaatgc acgatcagca agttcagcca ttttcgctgt
    29461 gtccaagcgt ttcattaaac tacgcgtttt aagaatagat gtcgcactca ttgagaactc
    29521 atctagaccc atacctacta gcaatggaac agctgtttga tctccagcca tttctccaca
    29581 catacctgcc cattttcctt cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa
    29641 aattgatggg ttatatggtt ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc
    29701 cattgtgtat tgaatcaaat cgttcgtacc tacagagaag aaatctactt cttttgcaaa
    29761 cttgtctgct aagactgctg ctgctggaat ctcgatcatg atgccgactt ggatcgtatc
    29821 agatacttcc ttgccttcac ttatcaattt ttgtttttct tcttcaaaga tcgcttttgc
    29881 tgcgcggaat tctttgagtg tagctaccat agggaacatg atacgtaaat taccatgaac
    29941 agatgcacga agcaatgcac gcatttgtgt acggaacatt tcgtcgcctt gttcagataa
    30001 actgatacgc aatgcacgat atcctaagaa tggattcatt tcatgaggta attgcagata
    30061 tggtagttct ttatctccac cgatgtccat tgtacgaaca actacaggtt ttccttccat
    30121 accttctaat acagctttgt aagcttcata ttggtcatct tctgttggga agtctggaga
    30181 atccatatac aggaattcag tacggtacag accaactgct tctccaccat tattatggac
    30241 accaactaga tcttttggtg ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc
    30301 tgtgacagtt tctgcatttt ttaatttttc ccattctgct ttttgtgctg cgtaatctgc
    30361 acctgctttt tcaaattcag ctttttgttc atctgtagga tcgatgatta catcgccttc
    30421 gatcccgttg acagccaaaa tatcgccttc tttgacttta gcagtaattt cttttgtccc
    30481 aacgattgct ggaatttcta aagaacgtgc catgatagca gaatgtgaag tacgtccacc
    30541 aatgtctgtt acaaacgctt ttacaaaatt gcgatctaat tgtgctgtat cacttggagt
    30601 caagtcatgt gcaacaacga ccacttcttc gttgatcatt gatggatttg gtaaagtaac
    30661 gcctaataag tgcgctaaaa tacgtttagc aacgtcacga atatctgcag cacgctcttg
    30721 catataagca ttatcttcca ttgcttcgaa cataccgata tacatatccg ttacttcttt
    30781 tagcgctgat tcagcattta cgctgttgtc tttaatattt tgcttgattt gacctaccat
    30841 ttctggatct gaaagaacca tcaaatgggc atcaaatact tgcgcttctg cttcacctaa
    30901 gctttgcgct gctttttcac gaatttgctg caattcttca gtagatttag ctaatgcagc
    30961 atccaaacga gcttcttcag ccgcagtatc ttctactgtt gccttactga atgttaaatc
    31021 cggttgaact agcaggtaag cttttgcaac ggctacaccg tcactagcgg caatcccttt
    31081 tagcatttca accattattc agataagcct tctttcttca ttgtttcaac gattgctgcc
    31141 attgcatcag cttcgtcagc accttctgca gtaatagtaa catcagaacc ttgacctaca
    31201 cctaaagaca taacgcccat gattgatttc aagtttactg atttaccttt atactctaag
    31261 tttacatcag agttaaattt gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg
    31321 atcccagttt ctgctactac gtgaaattct ttcttttcca tattaatcgg tctcctttaa
    31381 gtgagtgttg tttttatgtg agggttttct tttttagtaa ttcagagaat cctaacaaat
    31441 gccacccttt catctgtaaa gattaacatt ttttttcgtt agatacaaca gttttcattc
    31501 aatttcagat aaaagtattc aaattattcc ggattctttt tcatcctttt cttcacattt
    31561 tataacaaac gtgtttattt cctttttctt gtcgctttct cttctcctga cgtttatcat
    31621 tctttccttt gaccctcttt gttttcatat gaaacgaatt acttgcttcc ctttttattt
    31681 ttagtataat aagagtcaaa ggtcaggaat ggtcaataga gatttcacct tttctacctc
    31741 ataaatctct taaaaaacta ggggttattc ttttacctca tgaaaaacga gatgtaaaat
    31801 atggatgtac ccatttttgt gaaaggacgt gaatgattca tgctttgtca aaactgcggc
    31861 aagaatgagg caacgattca cttgtatgcc actgtcaatg gtcagcggac acaactcgat
    31921 tactgtcaga gctgttatca aaaaatcaaa aatcaacaaa atggaggtct gactaatatg
    31981 gcccaaaatg atccatttgg atttggcagt ttggacgatc ttttccgttc catgtctcgc
    32041 caaatgcaac agcaaggaaa ttatgagcaa acaccaccaa cacaatttgg cggaaacaac
    32101 aacttcaatg gtggtcagcc acctcaaggc aatacagaag gtttattggg agaatacgga
    32161 atcaatatca cagaacaagc acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac
    32221 gaaatcaaac gagtcattga aattttaaat cgccgaacca agaacaatcc tgtcttgatt
    32281 ggagaaccag gtgttggtaa aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat
    32341 ggtgatgtac cgcaaaaatt aatggacaaa gaagtcattc gtttagatgt cgtatcttta
    32401 gtccaaggta ctggtatacg aggacaattt gaagaacgta tgcaaaaatt gattgaagag
    32461 atccgtcaag cagaaaatgt gatcctattc atagatgaag ttcacgaaat agtcggtgct
    32521 ggttctgctg gtgatggaaa tatggatgct ggtaatattt taaaaccagc tttagctcga
    32581 ggagaattgc aaatggttgg tgctactacc ttaaatgagt accgcatcat cgaaaaagac
    32641 gctgctttag aacgtcgtat gcaacctgta cgggtagatg aaccaactgt cgaagaaacg
    32701 atcagtattt tgaaaggttt gcaaaaacgt tatgaagact atcatcatgt aaaatatact
    32761 gatgaggcca tcgaagcagc cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg
    32821 ccggataaag ccatcgattt gctagatgaa acaggttcta agaaaaa
//
LOCUS       DACEOE010000010        30729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEOE010000010 DACEOE010000000
VERSION     DACEOE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.010"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(121..3399)
                     /locus_tag="IW774_000445"
     CDS             complement(121..3399)
                     /locus_tag="IW774_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1152824.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3670..4215)
                     /locus_tag="IW774_000446"
     CDS             complement(3670..4215)
                     /locus_tag="IW774_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1152825.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4227..4919)
                     /locus_tag="IW774_000447"
     CDS             complement(4227..4919)
                     /locus_tag="IW774_000447"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1152826.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(4938..5222)
                     /locus_tag="IW774_000448"
     CDS             complement(4938..5222)
                     /locus_tag="IW774_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152827.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5235..5798)
                     /locus_tag="IW774_000449"
     CDS             complement(5235..5798)
                     /locus_tag="IW774_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1152828.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6026..6688
                     /locus_tag="IW774_000450"
     CDS             6026..6688
                     /locus_tag="IW774_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1152829.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            6812..8008
                     /locus_tag="IW774_000451"
     CDS             6812..8008
                     /locus_tag="IW774_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1152830.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8109..9485)
                     /locus_tag="IW774_000452"
     CDS             complement(8109..9485)
                     /locus_tag="IW774_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1152831.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9522..10388)
                     /locus_tag="IW774_000453"
     CDS             complement(9522..10388)
                     /locus_tag="IW774_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1152832.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(10477..11502)
                     /locus_tag="IW774_000454"
     CDS             complement(10477..11502)
                     /locus_tag="IW774_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1152833.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11520..12194)
                     /locus_tag="IW774_000455"
     CDS             complement(11520..12194)
                     /locus_tag="IW774_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1152834.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12378..13271)
                     /locus_tag="IW774_000456"
     CDS             complement(12378..13271)
                     /locus_tag="IW774_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1152835.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(13345..15042)
                     /locus_tag="IW774_000457"
     CDS             complement(13345..15042)
                     /locus_tag="IW774_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1152836.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(15035..16348)
                     /locus_tag="IW774_000458"
     CDS             complement(15035..16348)
                     /locus_tag="IW774_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1152837.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(16348..19866)
                     /locus_tag="IW774_000459"
     CDS             complement(16348..19866)
                     /locus_tag="IW774_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1152838.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(20159..20617)
                     /locus_tag="IW774_000460"
     CDS             complement(20159..20617)
                     /locus_tag="IW774_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1152839.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(20891..21433)
                     /locus_tag="IW774_000461"
     CDS             complement(20891..21433)
                     /locus_tag="IW774_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1152840.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVICINCTNKA"
     gene            complement(21435..22052)
                     /locus_tag="IW774_000462"
     CDS             complement(21435..22052)
                     /locus_tag="IW774_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1152841.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSFYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(22049..22903)
                     /locus_tag="IW774_000463"
     CDS             complement(22049..22903)
                     /locus_tag="IW774_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1152842.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(22900..23811)
                     /locus_tag="IW774_000464"
     CDS             complement(22900..23811)
                     /locus_tag="IW774_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1152843.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(23852..25186)
                     /locus_tag="IW774_000465"
     CDS             complement(23852..25186)
                     /locus_tag="IW774_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1152844.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            25394..26224
                     /locus_tag="IW774_000466"
     CDS             25394..26224
                     /locus_tag="IW774_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1152845.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(26468..26717)
                     /locus_tag="IW774_000467"
                     /pseudo
     CDS             complement(26468..26717)
                     /locus_tag="IW774_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26908..27102)
                     /locus_tag="IW774_000468"
     CDS             complement(26908..27102)
                     /locus_tag="IW774_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152846.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(27607..27792)
                     /locus_tag="IW774_000469"
     CDS             complement(27607..27792)
                     /locus_tag="IW774_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152847.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(28642..28983)
                     /locus_tag="IW774_000470"
     CDS             complement(28642..28983)
                     /locus_tag="IW774_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1152848.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(28983..29213)
                     /locus_tag="IW774_000471"
     CDS             complement(28983..29213)
                     /locus_tag="IW774_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1152849.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            29500..29838
                     /locus_tag="IW774_000472"
     CDS             29500..29838
                     /locus_tag="IW774_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152850.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(30043..30282)
                     /locus_tag="IW774_000473"
     CDS             complement(30043..30282)
                     /locus_tag="IW774_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1152851.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(30258..30605)
                     /locus_tag="IW774_000474"
     CDS             complement(30258..30605)
                     /locus_tag="IW774_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1152852.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         9008 a         6560 c         5256 g         9905 t
ORIGIN      
        1 cagcaaggag aacctcatct ataaaaagat agaaggaagt gagacatcta ttggtctcat
       61 ttccttctat ttttactttc ctcagtcaat tgttttacat cataaaagga gaaattccac
      121 ttattttttt cgctgtttca tgacataaac aactacaccg attaacaaaa ttcctccagc
      181 taatgctagc aacgggttat taaattctcc cgtttttggg agtccttgag tgccattttt
      241 ttcactttct tttgctgtcc tatccggatg agaaggtttt ccatttggtt ttccatttgg
      301 tgattcattt cctggatttt ctctttctgg tttgtcattt cccgggggca catcggcagt
      361 ttctcgcaca gtgatcgctt tttgctgggt aacagattct gtatgagcag accatttttc
      421 cccatcaaat atttccgaac gataagtgac ttctagcgta tattctcctt ttgaaggtgc
      481 tgaaaaaata gcggtatgat tagaaaattg agcacttgac tgtgtttcaa cgatttggat
      541 cttttctggg aaataacgca tatcttcaaa gacaggcgtt ccttgatttg gtgtgaaagt
      601 tgacacaaaa actgtaaaag gcttcagttc aatgatattt ccttctgcat tctccgccac
      661 accatttgct tcttgtataa taatcggatt attttgttca tttggttttg tctgatttgg
      721 ttcttgtacg attgcatact ctggatattc cgaagacggt gaggtttctc cgtcaaaaat
      781 agcacctaca gataatggaa gaccatccac tctttccttt tctgtttgac gattccccgg
      841 tttaatctgc atcagtccat gaacatggat acttccattt tcatagcgcg taacaggctg
      901 ctgaccttct ttcgaacatg tatttagaga ctcgatccaa tcttttgata tggtatgtcc
      961 aagagtattt tctgattgtt tctctcgttc attccaatag tagttcaacg agtggtagat
     1021 ttgatcttcg ttttgattca atgggcggat ctgctctctc atctcaagat tctctgttcg
     1081 gaaagataag ataccagaag catggtagtc tgtgtttcct gctgaagagg tataaagagc
     1141 cacattatat gaaccgttat taaaacttgt actgctattg acgataatat ctgttccgct
     1201 attggaatcg attcctttcg caagattttc gaaagacaaa ctattgatca attcatgagg
     1261 gcccgttaaa gaagaaccgc ccattttgaa tccattaccg ttaccttctc cctcaattcc
     1321 tggtacccag ccgtttcgat aagcaactga gtttttgatg attacttttc cgatcgaacc
     1381 tgtttcattt ttagcaaaaa ggtcccaacc gtcatctgca ttatgatacg cgatacaacc
     1441 gtcaaataca tttccttctc ctattgtcag tttggccgca aatccatcgg catcttcaaa
     1501 tcctggatcg gcattttcaa aagatgtaca gttcttgact aagttatgcg cgggccatgc
     1561 ttcgaaagga tcatttgagc taccgctgat ttggattcct gtatttccat tccgataagc
     1621 atgaatctct tcgatcaaat tatgatttcc agaaatctgt aatccttttt gcatagctgc
     1681 actatttgta atgtcaaacc ctcgtaaacc ccagtagtga ctgaacagtg tcatcccatt
     1741 tccagttcct ttaaaatcaa gtacaggacg cttgttttct ggatttttct ctgcaatcaa
     1801 ctgaatcggg ttttctcttg tcccgttaac gccttttgga atcgtcaatc ctctttcgaa
     1861 ggaataagtt ccaggcgcca tgacgattgt ctgcccagca tatgcgtatc tcaatgcttg
     1921 tgtcagagat aatggatttt gtctgcttcc gtttcctgca tctgaccctt ctggtgttac
     1981 ataaactgtc gaatatttag gtttacggta gtccactgta tgaagaatct cttttgtttc
     2041 ataactagtc atctctacgt attcttctgg cggatagttt ttatctggcg taaaactgaa
     2101 agtaaattcg ttcgttcctt ccatcagttt tgtctgataa tcaaattccc gattcgcagt
     2161 gatagccaag ttttttgcgt tcttaagcac ctctcctgtt gctttatttt taattgtcaa
     2221 taatccgtca gcatttgctc taaaggacat agtatgatct tggtttcctg aatgattact
     2281 agaggtcaca gtgtaaatcg gatcaatcaa gcgagtaggt ctttcttgcc caggaggatc
     2341 tgtcgcagga tctgagtaag caacttcgat tccttctacc ttgatcgtca tattccttgc
     2401 tgcagcaaat cctgcataaa taaccgattc atctgaagcg tataattttt cccagtcata
     2461 catgatgtca gaagccattt cttctcctgt ttcaggatcg taataatatg cctctaaacc
     2521 agtattcgtt tttttcatct ctacgtccag tttagtaatc gagttcgtgg ccggaaacag
     2581 attcccatga ccgtttccga atatattata agaacctgtt tctttttcca agaaccctgc
     2641 tgaagtctct aaaggtgttg tttccactga gacactccca ggagctggcg cagcttgcgg
     2701 atcttcagat gatattcctg tgattgcccg tgtccccaat ccaagtcgca tcgtatattt
     2761 gctgccgctc gtgttgacca cttcttgtct atccgagtcc cagacatatt cgatccgact
     2821 gcctagtatg gcaaatgaat ttgaataaaa tgcctgccca aacaaatgat tttttggtac
     2881 tgcgtctctt accattaaag caaatccttc ttgggcattc gtataggtcc aagattctat
     2941 ttcgatcgtc gcccgcatcc taaagttgat gtctttgggg attgcttgat aataataggt
     3001 cattccatct gatccgcttg actggaattt tccgccgtta ttcgtggaag agagccgcac
     3061 gccgtcttct aatggttcaa tggcatacct actagcactt gtgctttctc cgaaaacaac
     3121 ccctttccac tcttcatttg cttcttcaga ttgctttttt gtcttttcat tttctgattc
     3181 tttcatcata ggatcatttc cttcttccgt atccatatct tcactagttc ttggtatttc
     3241 ctccattgtt gacatccctt caatgttcac gtgtgcattt tcttcgttca cagactgttg
     3301 ttcgttagca gcaacttcaa cgacagaaat taaaaccggg gtactgagca taaggagatt
     3361 catcattagc gcactgtatt ttatacgttt ttttaacaag tgtattcctc ccgtaacaaa
     3421 ttagaaagcg tttacttaaa gtattaccta atttattttt actgtcaata aaaaacaaaa
     3481 ttataataag tgtattttat aaaaagaaaa aatataactt aagttgttga taataaagag
     3541 atttattaac gtaaaaagaa taagtttttt taaaataacg tataaaaaag agcataaaac
     3601 aaagtcgttt gttttatgct cttcttttat tattttgtgc tttttatttt atcaaatcac
     3661 tttgtttttt tataaaattt ctgcgcctag tgtggactca aaatgtctca atgcccattc
     3721 atgtccaacg ggatcgaaac ttgccacggc atctttgaaa atatggagtt tgtatcctag
     3781 attatacgca tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata
     3841 aatatctgtg atctggcgtt ctcgcaaacg gatgtctaga tctgttccac taaaagcgga
     3901 ataatgccgt ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact
     3961 ttttaaagag ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa
     4021 gcggttctcc ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa
     4081 atccttttga tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc
     4141 tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga
     4201 gatcaatgcg ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt
     4261 tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga
     4321 tctgctgtgt cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt
     4381 tgtccaacag cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt
     4441 tctttgattt tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt
     4501 gcgttcaaat tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct
     4561 tcataatata gaggcattcc tggtaattgt cctgggttgt agccaaatcc tgttgcatct
     4621 gcatcaaaat aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg
     4681 attccgccag ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga
     4741 cttgtggtaa tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg
     4801 ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt
     4861 aggttctctc tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc
     4921 tttttaattc cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg
     4981 ataacaataa taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg
     5041 ctgatccttg ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca
     5101 atcttttttt cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg
     5161 cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc
     5221 ataagccgat ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc
     5281 gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga
     5341 tgatacaact ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc
     5401 ccttgtgcat gataaatatg aaggacttca tttgcaaaac ctggtgaaag atacatcgaa
     5461 gtaagatggc tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct
     5521 gtcatttcgg gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc
     5581 actttttcta aaggttttcg aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa
     5641 gcaatgatcc cgacaccgcc aggatgaaat accagttctc tcttcgaagt tcctccatct
     5701 ggcaaacgaa cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga
     5761 ctgattgttt tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta
     5821 atcataactg cttttttttc atttttcaat ttttctcaaa aactccttat tagtttacca
     5881 tagataaacc ttagattttc tctaaatata gaaaatcctt ctttagtccg atactcttta
     5941 tagtagtccg tatgctaaaa taagtatgta atcgaataac gaaaagaatt caatactcaa
     6001 cgataaatga catggagggt ttattatgat aaaacgaaat tggccgtggc ttctagcatt
     6061 ggtcttgcta tttctctttg tccgaaacaa tagtttttct tttgttctag gacttttatt
     6121 gataggtgta gttttagttt tgtggggact ctttggttcg cgcaggaaaa agggaaaaca
     6181 agaagaaatt cctgcactga ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac
     6241 agcgaatgaa atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca
     6301 attgcaaatg aatatgcagc agacagccaa acttaaagca atcgatctcc gccatgatcc
     6361 agtcaaagca gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc gactgcatga
     6421 agcaagtcag tttctttata cgcatctgcc gaatctagtt gatttgacaa ataagtataa
     6481 tgagatcaat gaccatgaga tcaaaaacaa acagacctat gaaaaactag aagaaagcgc
     6541 gcagatcatc gaccagctgt cagctttaat cgcacaagat tatcaaaaat tcgttgctga
     6601 ggatctagat gatatcgacg tagagatatc tgttgcgaaa cagagcttaa agcgtgataa
     6661 taaatatgaa ccagaacaaa aggattaact cttttgttca taaaagaaga ctcggaacat
     6721 tcagatgatg tctggctgtt tcgagtactt tttctaaagt agagacattc atgtaagaat
     6781 cgagaacaga ttcaacaagg aggaattttt tatggatcat aaaccgactg aaacaaaaga
     6841 tgttacacca gttaacgata cacttgaaga tttattgaac aacccgtttt caactccaac
     6901 agatacgctg accacttcac aacaagcgga gattgatcag ctccaagagc aacagacagc
     6961 tgctcgttta attgacaaac taccggcgga acgccaagaa caagctaaac agctagcagc
     7021 aaaaatcgat gctaatgatg cacagtctgt catcagctac ggatcggctg cccaagcaaa
     7081 attaagtgaa ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt
     7141 aggagattcc ttaactgaac tgatgtatcg tctccaagaa gccaatcctg acgaactgcg
     7201 tgccggagaa gggaacatct tccagcgtgt cttcgggaag gtcaaacaat cgatctatga
     7261 agtgacagca aaatatcaaa agattggtgc acaaattgac aagatttcag tgaaattaga
     7321 taaagaaaaa gacggtctgc taaaagataa tctgatgtta gaacagcttt accagaaaaa
     7381 caaagactac tttgatgcac tgaatattta tatcgctgct ggagaattga aaatggaaga
     7441 acttcaaaca acgatcattc cagaagcgat gaaaagagca gaagaaactg gcgatcagat
     7501 ggatgttcag attgccaatg attacacgca attcctagat cgtctggata aacgtacgca
     7561 tgatcttcgc ttagctagac agatcacgat ccagcaagca ccacaaatcc gtttgatcca
     7621 aaacacaaat caggccttgg ctgaaaagat ccaagcatct atcgctacag ccattccgct
     7681 gtggaaaaac caagtcgtga tcgcattgac cttgcttcgt caaaaagatg cagtaactgc
     7741 tcaacgccaa gtctctgaaa caacaaacga cttattgaag aaaaattcag aaatgctgaa
     7801 aatctcagcc atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct
     7861 tcaaaccact caaaatgatt tgatcgaaac aatccaagag acattgcgta tccaaaaaga
     7921 aggaaaagaa aaacgtcgtc atgcagaagt agagcttgga cacatggaag aagatctgaa
     7981 acaaaaactg ttggatttaa cccaatagat cacctttcgc ttgttactaa aacaaacgcc
     8041 caacaacagt ttattacaac tgttgatggg cgtttttctt atctttcttc taacttctcc
     8101 attggagtct atttctcatt catcttgctg tgtccatagc catagccaaa ataaatgatc
     8161 agtccaataa ccattacgat accgaacacg atccatgtca cagtttgcag acgaagcatc
     8221 aatacaatac aagtcaagat ggagacgatt ggcaatagcg gaacgaatgg cactttgaat
     8281 tctccagctt ttggtttgcc aaacattttt ctcaacttca gtaagcctag actaacaagc
     8341 atcaaatagg cgagcgccat gatattcgtt agttccgcta atacattcaa tgggaaaaat
     8401 ccggcaaaga atacagctgc cacaccagcg gcataagtcg cttttttcgg tgtacgatgc
     8461 ttttgatcga tttctgtcat aaacttagga agtaagccat ccttgctgac agcaaacagt
     8521 aaacgagcaa gtgaatacat catcgagatc gttaccgtca gtagcgttaa aattgcccct
     8581 acagcgatca ccgcgcccac tgttccatgt cctacatacc tcattgcaaa agccactggg
     8641 tcattgactc ctagttcagt atacggaacg attcctgtca agatcagcgt cacgatgatg
     8701 taaaggacag tagcaatcag tatcgaacca atgattcctc ttggcacatc tttttgcggg
     8761 tttttgactt cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca
     8821 agagctgcac catctagcac accggaaaaa ccaaaaggca tgaatggctg ccagttatca
     8881 ggtttaacga aaaatattcc tacaatgata aacaacgcga tgataccaaa tttgacatat
     8941 accatggcat tattcaaacg taatgctttc ttggcttcaa gcgacacgat gtaagtgacg
     9001 aatatcacta cgagcaccgc aatcaagtca atgtatgtac cattttccgg attgtacccg
     9061 ccagttaatg cttgtggaag ttcggcccct aaacttttca aaaaaccttg catatagcca
     9121 gaccatccgg aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct
     9181 gtcagccagg cagcaaattc accaaataca acatacagat aggcatatgg cccaccaatc
     9241 actggcactc gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac aacgattgca
     9301 gctaagacaa atgaaagtga cagtgctgga ccagcatttt gattggccgc aacccctgtc
     9361 accacaaata ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa atcagacgtt
     9421 tttagttctt ttttcatctc actcgtatga ttgagattga tctctttctt acgaaacaaa
     9481 tccattgaag ttcctcccca ttttaggttg gaaaacagcg attaaactgt tttcatatac
     9541 cctaaaagga taccacctaa aatgactaaa aggcaaccaa caataacata aatgagctct
     9601 tttttcgttt ttctttcgcc aaggatgaaa atcccaccta aagtagagat aatgatcccc
     9661 atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt tgactgttag aagcatgcag
     9721 atgtttccta agccccataa taatccacag atcatgtttt tccacacaaa ctgatcgact
     9781 ttaactttac ggaaagcaaa gaagcttgcg cctaagatca ttccgatact ttgaggtaag
     9841 ataattgaca tcgcatccaa attcgcccag ttgacgatga tggtatatac accataacca
     9901 atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat caagcatttt attatctgta
     9961 tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac aagaagagct
    10021 aaagcaataa taccgtatac ataatctttc gtttgtgtcc attcatggaa gaaaacggct
    10081 cccgcaatcg tattcaaaat taattgaaaa ccagtagaca atggtagccc tacagaaaca
    10141 cccatgtact tcattccatg gaactgtcca ttttgtccaa cactccaagc taagccggat
    10201 aaaaatccga tcaagaaaat ccaaggggtg attacaggac taacaacgaa aaagacaact
    10261 aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt gttgattggc atcgccacca
    10321 attttaccac ttactaaacc aatactaccc catgccaaca tagggactaa tgctattaaa
    10381 atttccataa ccaatctcct tttatttcac atgataagaa acttatcagc gaaaacgtta
    10441 acaacacaag cacttttttt cgtttctaac gattttctaa cgtttttcat tataaaaatt
    10501 taagattggg aaaaataaag tcattgttgt cccttttcct tcagttgatt cagccgtcaa
    10561 atggatacct aatttttcgg ccagattttt tgctaaataa agtcctaatc ctgttgaatg
    10621 tgttttgcta agtcgaccat ttctcccagt aaatccctta tcaaagatcc gtttcaagtc
    10681 ttccgctggg ataccgatcc ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt
    10741 ttctatgctc acaatgatgt ttccgtgatc cggcgtatat ttgatggcat tactaagcaa
    10801 ttggttgaag ataaaagcga tccatttagc atcggtcaaa acagtatgat cctcaccaac
    10861 gatctcgtag cgaatatttt tttggataaa ataatttgcc tgactgcgta tcacaggctg
    10921 aatgattcct tttagtccat attcttggat gaggtagtcc cgcgagaaac tgtcgagtct
    10981 tgcataatac aaaacttgtt ctacgtattc atcgattttt gataattcat tttctagtaa
    11041 tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga actagcaatt tagaagcgga
    11101 aagtggcact ttgatttcat ggacccatga gtcaatataa tctttttggt cttgttgatt
    11161 actgattgct gattgcatta cttcttggtg ttcacggatc agctgattga tatattcttc
    11221 taccagtttt tcttcttctg tcctcccacc actcaaataa ttttgcaaag gggaagtttt
    11281 ttgtatgtcg gctaattttc cccaccatct tctcttgctc aagtaaccac ccagcagata
    11341 aaaaatcaga aagacgccct caatcaaagc taaataagca attgtgctca aatcaacttt
    11401 gatgtttggc gccagccaca tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa
    11461 gatcaatgtc cactgatctt tcaaatattt aaaaaaattc atcttaaccg ctcatccttt
    11521 cagggaacga tatatccctg tcctactttt gtttcgatat atccttggat acctgcctgt
    11581 tcgatttttc ggcgtaaccg attgatgttg actgtcaatg tattgtcatc tacaaatcgt
    11641 tcgtcatccc acagtgctcg cagcaatttt tcccgactta agatctttcc attgtgcttc
    11701 atcaaaataa ataatagacg atattcattt ttgctcaaat caatgatctc tccgttgatt
    11761 tccatacttc cgttgtcaac attcaacgta atgccattgt ggctcatcat ttcactagat
    11821 aattctgtgt agttgtaaga acggcgaagt aatgcattga tttttgcaat cagtacatcg
    11881 atttggaaat gttttgtcac aaaatcatct gcccccatgt tcatagccat gatcatatcc
    11941 atattcgtat tcctgctgga gataaaaatg atcggtactt tcgatacttc acgtattttt
    12001 tgacaccaat aatagccatc caaaacggga agattaatat ctagtaatac aagctgtggt
    12061 tcttctcttt caaagtctga gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat
    12121 tgccatttct caagttcctc catgatcaat tgtcgaatga tctcttcgtc ttcgactacc
    12181 atgatttttg ccataaaaaa tccctccatc tttcttcttg ctactacaat atcaaagaag
    12241 cccttgtgaa acaagttggc ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac
    12301 tggaaatatg cgatttaatc gcgtttgtct cttccaagca gcttaaattt attatgttat
    12361 tcttaataat tggtctatta tctgttattt ttctttcgtt gtttttgtct tttaaatttt
    12421 cgataacacc aataaccgaa aagcgttagc actagcaata tacctagaag gagtataagt
    12481 aatcgataaa gatggtattt ttttgtctgt tctatctttg aagagacttc ttctgtaaag
    12541 ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg
    12601 ataagtgtga ctagatcctg cttttctcta attgctaatg tatttgtttt tgttggaagg
    12661 acaacaattt tctcaacgac ttcataagcg agcatccgac catacacttc aagatagaat
    12721 cggtcatctt gctccatttc ttcaagatcc gtaaagagtt ttttctcagc aataccgcta
    12781 tgggcggtaa tcacggaatg tgtactctct cctccagttg gaaaagaggt gccttgcagg
    12841 actgtcgctc ctctatctaa caaaagatca ttcgtttcat caaataaagg caagctaaca
    12901 ttgattttcg gaatatagat ggctccgatc atatgttgtt cgtaatattc ttttcctgga
    12961 ttttggacat ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat tcctgggatt
    13021 actgatgtat gagcaagcgt ctgatttttc ttttcatgca ctgctaattg ttgctcatgt
    13081 ttttcttctt tatcgcgttc aagctgcttt tgataattct cgatccttct ttgatccaaa
    13141 taattgctga gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac
    13201 atcactgcca taaataattg taataaaaga ctgatttggg tttgaggtct attacctatt
    13261 ttctttttca ttttcttcac cttcttattt aaaaaaactg cagcaacctt tataaaaccg
    13321 attgctgcag cttatttttg ttgcttatac ttcagacttc attttagatt tacggtacca
    13381 gctgtacgct ccaatcatca aacttattcc gattagtaag aatgccaaga gcccgttccc
    13441 accagtagca ggaagtgacc cgcgtttcat attcggtact ctttctggcc ctggattttc
    13501 tactcctacg atctgaattt cttcattata gcttccataa gagatgataa attcagtaaa
    13561 ggctgcctca cctgttggaa gtacataacc ttctggcgcc atggtctctt tcatttgata
    13621 ggttccataa tctaaccctt gaacctcaaa gtatccattt ccatctgcga caaattctgt
    13681 tgcttgtccg cttgttgccc actcaattct atctgctcct gattcataaa cagctaaagg
    13741 cgactcccct ttataaaata ttgcgtattc agaacggtct tcattccaca attcgaatct
    13801 tgcatcttta agcaattcac tgcttgatgc atcatatttt tcaaactttc ttccacctgt
    13861 tgtgactttc tctggcggct ctactccttc ttcataatca aattcatcat ttcgtccgat
    13921 ttcaataacc gctgtgttgt tatggaaatc atccgcttct agttctgtgg aaaccttcaa
    13981 attatacgtg atcgttaaat ctcctccacg tgcatctgct aatctaccta aagtatctac
    14041 tttggctact ccattttctt ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc
    14101 tactatataa tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg
    14161 atcataataa gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc
    14221 gcttccgttt tcaaggacag aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga
    14281 agccgttttt cctctttcta gatcaatata agaaatcgtt tcttgttctc cagcttcatt
    14341 aataatcgtt acttgtctaa ggtcgctttc ctctacattc atctgtttat ctgcttctct
    14401 gatttcgttt ttcggataca aatgcacatc tgtattgtat gtgtctttcc atgtagaacc
    14461 atcccacacg ccatccacca tatcaggcat catcactggc atcataacaa ccattggaga
    14521 cgcgattctg gaaatatgag ctggcgaata agtttctaag aaaagataga ctttatctct
    14581 agaagaaggt gcttcttccg tcacttggac ggtagagaat accgcttctc cagcttcatt
    14641 cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg
    14701 tttttgaatc cattcaatcg cttcggcaga attaagaccg tcttctcgtt ccggtgttaa
    14761 aggattatct cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat caaaatcgac
    14821 tcctggaata ttttcttcac caaaatccgt agaaaccaat ccgctattcg gtcgttcttc
    14881 cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc
    14941 gacttcttcg gctttgacta gttttccgtt cccgaaaatc cctattcccg cagtaacgag
    15001 taacagtaca ctcattcccg tgacaatcat ttttttattc atttttttct ccccgttctt
    15061 tttttattaa taaaacagca cctaacaata aaagaattcc tagtaaagaa atcaacgttc
    15121 ttgcttcatt cgtttgcggg aatacggacc attttcctga ttgacctgga ttgcttggac
    15181 cactcggctc tttcggtttg tccgttgttt cttttacggt ctcgttataa acacgtggtg
    15241 ttgcggattc ataggcagag actggaactt tcccggattc taattcagcc atctcctgtc
    15301 cgttaactac tacatattgt ggttggcctt ttgcatccac ccaaaattca gggatgatga
    15361 cctcgattct cttacttgct tcatccatct cgtaaccttc cacacctttt aattcttcaa
    15421 aataataggt gccagaaggc aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg
    15481 aaatgaattt gcttacattt ttgtcattca aaggatcatt gtctgctggt ttgatccatt
    15541 gatttttcaa gtcattagca ggagccatat ctaaataata tttcccccct tctatcatct
    15601 gatatagagc aaaaacagca ccttctaatg gttttccttt ttcagttgtt cctctttgac
    15661 gtccatactt gaagaagtat ggatctctta gataaccgac atttttagga tagatatgaa
    15721 tctcttgcag ttcctcggtt gattctgtcg ggtgataaat tggcaaaata gcagcaattg
    15781 ggatagctgt ctgatcgata tcaacatcca atccaacttc tggatcaatc gattcttcga
    15841 aaatcaaata aactgcatcc cgatcttggt ttttcttagg tgctttgata cgtgcaatcc
    15901 cgtctcctaa tccgccaacc gtatctttcc cagttgttac cgaagtccct atttggacaa
    15961 gcctttcttc tgtgattagc tgacggatat tgtttctgtt cgtttcagcg atttgctgga
    16021 cgaattcttt aggagtcatg ttttcagcag cttttttttc atgataataa tcggtcatgt
    16081 cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg tgaaatgata tctgctgttt
    16141 cccctgcttc ctcagagata gccaagccat cattttcatg gtattcaaat tgatcgttat
    16201 aatcgatatc tcgaatcatc cgtttatgca acacgaattc cacgacatct tcccctgatt
    16261 cttctgccga catcgcatgt ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt
    16321 gaagcatcaa acacaacagc tttttcatta ccttgctccc tcctttttag attgtctgac
    16381 aaacaaacca taggctgctc ctgcaagcgc catgaagaat gcagcaagca ggatatagaa
    16441 taatcgtccg gatcctcctg tagcaggcaa cggattttca ggatcgttcc cgacactaag
    16501 ctgtaatctg attcgatttg tctccaaaag ttcatattct aactcttgct gtagatcagg
    16561 cgtctctgct tcttcgtttg gataatacga aatctcaact tttagtgtac ccgcttcttt
    16621 tcctggaact acccgatcat tttctatgtc aggttcttct cgataactaa tttctaaaat
    16681 aaagtaacct ggaagttttc tatagctgtc aggtgacttc acttccttga tccgatagac
    16741 cccttccaac aagtctgaag cattgaatag ggaagaatcg tttggatctg gtgccaagcc
    16801 tccctcgatg atttcatact ctcctgcact attttgtttc tcaattgaaa attctgcgcc
    16861 ttcaagacgt cggctattca tactgtcaat tttcaaaagt tccaaatcga ttggcgctag
    16921 ttcatcttct aaggttactt gtagtacacc atcgatgatt tcatagcctt ccggcaaggg
    16981 agcagtttcg tcttcgatga ccgtatcttc aatgataaac ttaccttctt cattcagtgc
    17041 aaactggaac acttgatcag atagccgata cccatttggt gcgacagatt ccgagacctc
    17101 atattcacca atagacagat gcagaggttg gtcgtaattt aaatcgacat acttttctac
    17161 tcctacggca cgagaataga agtatccgat cccaggcaaa cgtttcgatt cttctggttc
    17221 taacggatta ccagtgtatg ctcgatacag cgggaaggaa gaatccccca ctttcctgat
    17281 atcgaaacct gcaccatcta atgtatggcc ttcatggtct aattttctaa ctcggatctc
    17341 ccgataatca aacgtattgt aagcagtgaa tgctacccat aaacgatcct cttcatctgt
    17401 tgtatacgtg agatattgcc aattttcttc acctgtttgc ttgagttgga tagtccctat
    17461 agcattatcg taaagtcgca agatccatgt gtcattttca tcaggttctt tgaaaccttc
    17521 tgggttctgg ctctcaatga ttttgtattc tgcatagttg gtagtttctt cactatcctt
    17581 ataaacaaat gaatctggct ctccgattgg atgaataact ttctcaccat ccacgattac
    17641 atatttcccg tcttcatcta ctgaatagaa cgtgagtctc cctttctctt cctctacttc
    17701 gttcacacca gaaagccctt tcgctactgg atatccgatt tccagtccgt taccatctgt
    17761 ttcataaagt tcaaactctg cgccattgat tggcatatta ctgccttcaa ttagtttatt
    17821 gatattcatg tggattggct tgaattcatt tcgcatttcc agtttgacat acaactcatc
    17881 atactctgtg ccttctatgt taggctcttg tacaacagaa agcttgtgat attctccttc
    17941 ataataggaa ttcggatctt taccttccgg tccgccgact ctcgaaccag atgtatattc
    18001 atctacaagc tcgtagtgac ctaacatctc atcttcgttg gctgcatatt ttttgatttg
    18061 gaattgatac gtctcatcta ctaactgata acgtggtggt ccagcgatct ctctgatcca
    18121 aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat
    18181 cgctattccg tccgtggtag ttttacttgt ttcgattggt tcggcttccc cactctctgg
    18241 attttggtca tataatgcaa attcgactcc agccaacggc tcgccatttt gacccttctt
    18301 gctgacttgg aaattacggc ctttatgttc gtaacgaatg gaagaagatg ggaaatacat
    18361 ccgtccagta gtgatatcat tctcataatc ctccaggttc tgaatatagg ccatattgtt
    18421 cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt tttacttggt acttgatcct
    18481 gaccatttcc ccttcaccta aagaaatcgg agaaaattta taagcttggt tttcccgtcc
    18541 gacatacgtt gtcggagtcg ccactacttc tggtggggct tgtccatgac gttcttcatc
    18601 ccatatttct ggctcttggt acttcatgcc gttttctcct ggatctagat ggtaaaactg
    18661 aatatctgcc gatctgccag ctccaaatgg tgagacatta aaaccagttt catcataaag
    18721 tgtggcttgc tggatcgttc tcttgaaggt agaagagata tcctccaagg ctgtaacaat
    18781 gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc
    18841 aatatttttc aatacatttc gtccaatagc atctagtcgt tgtctcccgc taacgtttcc
    18901 atcgattcct aaaccaatgg agaagatact tggcgggttc tcttgatact tgtttttcag
    18961 ccaatgatga taagcaactg tgttgatcgc agatgacttt tcttgcgaag taggactgcc
    19021 attcgtattg ttattagaag aaccatcacc aacccagtga gtcgctttat tatctccttc
    19081 ttttacctct ccataacgat agtgtacatt tacttcatct tctggacggt taaagtcaga
    19141 tgcataggta actggatgag taaaccctgc attagaagta ttcccagaaa actcctcaaa
    19201 atttctgaac aaaacgtcat tatcttcagc aatcttatct tccatcacag tccagttgct
    19261 ccaagctcca ttagtgctcg tacgataact tgactgaatc ggtgcataac tgaaggtcgg
    19321 tgtcccatct cccacaacga ttaggatttt ttctggattt ctattttctc cgccattgtc
    19381 tttatacaat gtttcatagc catttttaag ccctagtgta agcggagttc ctccgattgg
    19441 tgtacgattt aatgctgtat tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg
    19501 aaagatgtcc gaactgattt gctcatggtt attgatagct gtactattat ggtaaaaatt
    19561 caccataccg attcgaatat ccagactcgg gtcagagagc agatctttac tcatttcgtt
    19621 tactgcctca atcaacgcag catttttagt ttgactattg gtcccagcgg ttaattcact
    19681 catactcgct gacttatcta gaacaaaaac gatatcgatt ggactgattt cctttttctc
    19741 gcccccgatg acatctagat agacattgta aagggttgtt gcatcttcag gatgtagatc
    19801 agatgctgtg ctgcttggca gaacaggttc tacccatttt ttgatgtacg ccccgttata
    19861 atccaagtaa ctgttttcaa aattccgttc atttccccaa agctgaacgg tttgagctgg
    19921 aacgtttcta tcttcttcgc ctgcagttga tccattatta gtagcatagt cgtaatttcg
    19981 ccagttcgct cccccgtttg tataatcact atcatcgatc aagttctctc cgtcatatgg
    20041 cgagcctttg tctccttgat agcgttcatt gctttgtgtc caatttccta acacttttgt
    20101 atagtcattc gttgtaaact tcatctattc tttcatcaac caccacttga agaggtagtt
    20161 acccttcgtt cacatcgggg atcttttctg tttcaaaaat caatgtgccg gtttctgctt
    20221 cttttgaata aacttttccg acataccatt cttgaccatc gatcgtctcg acttttaggt
    20281 cttctccatc ttcatcatag gaggatagac ctgttccacg aacgtttttt acggtaccaa
    20341 tcccggcggc tgccgcatcg attgctaaac gtattctacc ttcattggca ctttcctttt
    20401 tttcaaaagc cagatgccat tcaagggttt cttctttttc aatatacttc ccttcaattt
    20461 ttgcatagtc ctcatctaag aatacctcat cctcctttgt ttctgttgac tcagcagcta
    20521 catccagggt cgtcatatga tagactgact ggaaatatgg tccaaatagc aaactgacag
    20581 ttactaactg caaaagcttc aattttaacc ctttcacatc cttatactcc tttcaaaaat
    20641 atttgtcttc tttcacaaca atcaacaaaa caatgcccat ctaattataa tttaatttaa
    20701 ttttaatttt aaatcaaatg atatcctttt tagttaaaaa acaccaatta tacaacattt
    20761 attattaaaa ttaaattata aaattttata acgttctcat ttaacaaaac ttaagttatt
    20821 aagacaaatg aataaacaac tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc
    20881 atttacgcac tcatgcttta ttcgtacaat taatacagat aacggcagcg cataggatgc
    20941 caataacact tcctaaaata gctcctccta aaatgtcagt aggataatgt aaaagtaaaa
    21001 aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc taatattcct gcaactggac
    21061 ttaattcgaa caacgcagct actgataaaa aggctagcgc tgtatgtcct gaaggaaaag
    21121 aagaagtctt cggtttactt gtgtaaagga caagccgacg atttttttca taaggtcttt
    21181 tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa aaaatagatc aaactgccga
    21241 taagaatggc tagaccatat tctctagtat caggtatcaa gactaaaaca gcagcaagga
    21301 gcaaccaaat aaaaccacca ttagagatat tactgataaa tttcaaaaaa ttgatttttc
    21361 gactatccgt tttccgttgc ttggcgaact tgttgataat actatcatcg attttagcaa
    21421 tcaaacgcaa catgttattc ctccttttta agaacctttt cttgcgtacg atggataaaa
    21481 agcaggactt gaaaataaaa caagaaactg aaacctaata aagcaaaata gattggtcct
    21541 gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg
    21601 aaatcattaa aaaacaagaa aaatgcagta atccaagaag cttctttttg ttccagttga
    21661 agcaagattt tgtaactaaa gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac
    21721 atcaatgtat agccaattcc cccaccccaa tagcggaaaa tctgataatc gatcaataga
    21781 agaagaaaat aaacacttgc taaactatct tgctttccat aaaaagagaa gttctcacga
    21841 aactttttcc agaatagtga accgagcttt ttctcttcat tttttttgta atgataaatc
    21901 atatacagaa ctgttcgact tgcagttcct aatgtgaaaa caggcaagac gcacaggaac
    21961 catagtagct gtaaatggat aagacgtgga atccattcaa tgatttctaa gaaataatca
    22021 ttggcttttt taccaatcga tcgattcatc atccttttga acctcctgtc aaatccatcc
    22081 cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg
    22141 ttgctcctgc catcagataa ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc
    22201 caatctgtaa cgtgtatttt tcttgatctt ggatatataa cagaggacct aagaaatcat
    22261 tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa
    22321 gcatcaaatg actccaaata tacaaatgat tcgctccgtc gatttcagca gcttcgatca
    22381 attcattatt gatcgataag taaaattgac gcatcagaaa tatattgaac gcattgccga
    22441 agaacgatgg aacgatcagg ggcaaataag tacctaccca gccgatcttg gagaataata
    22501 catattgagg gatcatcgtt acaaaccccg ggatcatcat cgtagaaaga accaaagcaa
    22561 acatcagctt tttcccagga aaatcaagct tcgcaaatcc ataagcaata aaagaatttg
    22621 aaaggacatt accgatcact actagcacag tgatgaataa tgtatttctt gcatagcgcc
    22681 aaaaaggaaa ggcggcaatc gtatccagat agttttccca tacgattttt tctgggaaaa
    22741 aggtcggagg ataacgaacg atttcttcca ttggtttcaa tgaagtaaac accatccaaa
    22801 gcaatggaat gatcaaaatg attcctatta ctgtcatgag gacataactt agtatttttt
    22861 ttgtttgccg attcacactg gtctttttga aataatccat catttgccgt cacctccaga
    22921 ataatagacc cacttaggtg caagcttcaa attgacaaac gtcagaacca acacgatcag
    22981 aaacaggatc attgacatag ccatcgcata acccatatca aaagctacga acgccttgtt
    23041 ccacatatgc agattataaa aaagcaaaga atttgcaggt ccgcctgttc catttttcgt
    23101 catgacataa gcttcttgaa aaatttggaa agaaccgatc gtagaggtga tcacatcgta
    23161 aaaaataata ggagtgatta atggcaaagt aatacttttg aactttcccc aaacgcctgc
    23221 tccatcgatt tctgctgatt catataaaga tctaggaaca ttttgtaatg tagctaagta
    23281 aagtagcatt gctcctccag aactccatat tttcatcaaa atcaacgcag gtttcgtcca
    23341 ttgtggatca aacagccaag ctggtccagg gatatggaac cagcccagaa atgtgttgat
    23401 caatcctgca gatggagaaa gcagctgcat ccataagatg tagactgcta ctcctgataa
    23461 gactgccggt aaatagaaag ctgtacggaa tacactgatt cctttgattt tttgattgag
    23521 tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata
    23581 aacggtgttg tataacgatt tccaaaataa atcatcgtga acgaacattc ttttgaaatt
    23641 tgctagacca atgaaattca tcttggatgt caaattataa tcggtgaaac taccaataac
    23701 tgaaaaaagc ataggcccta gcataaatac caagaacccg ataataaaag gcgagataaa
    23761 taaataggcc catcttgctt cttttttctt tcggatagat aaacgcttca aattttcttg
    23821 cctcctctgt acctagacag tcaaaaatgg attattgatt ttgcttgact aagttttcta
    23881 ctgctttttg tgcatctgcc aatccttctt ttggtgattt tgttcctaaa agaatctggt
    23941 caatctgttc attcactaat gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca
    24001 ccgtattttt aaagttttcg tccgccattt gatagatcat ttttccttta tccgttaggt
    24061 tgtcattatt cactaaattc tcattggctg tcttgctcgc catgatatca aaactttttt
    24121 cgccgaattt ttcttgtact tctggagaag aaagatattt gatgaattca taagaggcct
    24181 caggatcttt tgctccgtat ggtacttcca agacgaatcc gccaccccac gaccaatggc
    24241 ctgaaccttc ttctttttct gggagctgca tcacgccaaa atcaaaatca tccggtgcat
    24301 tttccatcaa actagagtaa taattgatat tttgggcacg catggctact aatccggaaa
    24361 taaatggatc agatacacca gacccaaatt cggcttctaa tttattgatc gtatttttcc
    24421 cataataatc ttgccagtct tggatccact ccaaggcttc cactttattt ttagtatcaa
    24481 tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt atctgcgttt agcgcccata
    24541 catcagcccc taaattccat aatggataaa aaccgatgcg tgtaaattct tttccttctt
    24601 ttttatctag tttacgtgct gtttcttcta attcatccca agttgtcggt agatcttgtt
    24661 cactgattcc agcttcttta aataactttt tattataaaa aatgacttgt gtatctgtat
    24721 tgaatgggat cgcataagga tcaccttcat attctactgt atcccaaagc tgcggataaa
    24781 aatccttaga agtgtctttt tctacatatt tggagagatt agttgcttgc tgtgcttctg
    24841 ctctttgggc aacggaattt atgtcctgta caataacatc gggtgcattt ccagctgtta
    24901 ttgcagataa agatttagtc cagatgtcac cccatggctg atacttataa tttacatgga
    24961 tctcgttttg tgattgattg aaatcctcaa tgatttcttc gatagcttct tttcgtgcac
    25021 cggatcccca aaaggaccag aaatcaatat tttttcttcc atcacttgtt ttttcagaag
    25081 aatttgctgc acttgaactg gcatcgcttg cattacttga tccgcatcct cctaataaaa
    25141 acaatgctga acttgccagc aaccctgtaa acaataattt tttcatattt tacctccaaa
    25201 ttttaatgta accgatgccg gttttaatat aacgatatca ttttgttctg aaaataacaa
    25261 tttataaggt atgattctaa aaaaattatt aatgatacca atactttata taattcgcga
    25321 gcgctttctt tgaatttttt aatctttcgt agcagaatga gaatgtatta aaaatagatt
    25381 aggaggaatt ttgatgttta gacaccagaa agaattgcag tttgaagcaa aaccagatcg
    25441 tccgaatcca ttaatcgcga aatatctgca ggagctgatc ggcggccaat atggcgagat
    25501 gagtgtagct atgcagtacc tgttccaagg ctggagctgc cgaggagaag aaaaatataa
    25561 agatatgctg atggacattg caacagaaga aatcggccac gtggaaatgt tggcgacaat
    25621 ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga
    25681 tccaacaatc ggtgccatta tctctggaat gaatccacag cacgcgatcg tagctggctt
    25741 aggacctcgt cctgctgaca gcgtgggcaa tccatggagc ggtgcctata tcattgcgag
    25801 tggcaacttg ttagcagact ttagagctaa tttaaatgcc gaatcacaag gaagacttca
    25861 agtagcacgt atttacgaaa tgatcgatga tcgtggcgta aaagatatgc tgtctgttct
    25921 ccttgcaaga gatagctatc atcaaaatct atgggaatgt gcagtgaaag aattagaaga
    25981 aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt gacaaagaac gaaaagacat
    26041 tgcttatgac ttctataact tctcagaggg agaccaaagt agtgaaggtt cttgggctag
    26101 tggcaaagca ctggatggcg aaggcgaata tcaatggttg aaagaaccta agatcgaagg
    26161 cgctgcacca aaattagaac cagtaacacc aaaaatgtat gggacacctc cattcaagaa
    26221 ataaatatct acccgtagaa acacccgttt tttgttccac acgctttgtg agcaaaactg
    26281 actaaagtca ttaatcataa ccacccttca aaagggtggt tttctctaga gctataagct
    26341 caaatcaccg gccagcgcct aaagacgctg gctttcatat tttatgttca agccatattg
    26401 cctttgccag ttttgcccac ctcttaaaag gtttatgtac tactccttag cccttaaaag
    26461 gatcgtctta ctctcgtaca ctcagcttat ctaacgcact atcatgtttt tcttgttctt
    26521 gaatgtactt tttgatggtc gcttcatttt gtcctacttt tgtcacatag tatccttcca
    26581 cccaaaagtg tcgataccca aatttatatt ttagatttgc acaatctctt gatgatctcc
    26641 tgaatattgg ctcgatattg attataaatt atttttcttc tatactttgg tgtgaataca
    26701 atgtggtatt tacacatcta ctttgtacgg gctaaactat ttccctttat tgctccaccc
    26761 gcatagcaag tggttttgta tttcaatcgc tcggcgcgct caattaggtc taaagacaat
    26821 aaatctaaaa acccgctgct tcactgacag aaacaacggg ttttcagaaa aaaatgaaat
    26881 gaaaaagatc tcttacggca atctatttta tttttttgat tttttataaa tggaaaatgc
    26941 ttcaagaagt ccaagaaata aagccgtata aaaacaagaa cctatgaaat ctttatttat
    27001 tagatattca acagaaatcc cgataacact agccaacaat atatttatca gaagtgtaaa
    27061 ataaggatgc tttttataga atagaactag tgttgttttc atagactctc ctttcttcat
    27121 atttccaatt aatcacaaat tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg
    27181 aaaaatactc ttcgactttc cctacaaacc aggccatctt gtgtttaata actataccaa
    27241 atctaaggta cctctaaatc taaattccat ttgtggcggt gacctccaat ttttaactct
    27301 tctatctccc atccagtata cataaccttg atacttcgac cacttatata tcgaacacac
    27361 aaatacctat ccattgcagg agtactacct aacttagttg ctacttgcat ctaaataaca
    27421 gctgaaaaga taggacttac tcttagtaaa gtatctgaac tattaatttt taaactatca
    27481 tttttatcct caactacatg cacatctgtt gcaacttcac tttctgtctc atccagataa
    27541 atgattaagg aatcgggaat tgcaatatct tcagctgacg ctggaatagt tgagctaaat
    27601 tagtcatcat atctttatta taaaataacc aacagcaaaa aatacaatga aaaacaaact
    27661 tggaactaat gtatcattaa taaatgttga aaaattcagc aactcattac tgtatgattt
    27721 tagcagccca accaacatat accctataaa agctaataag atggttagga atcctttaga
    27781 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
    27841 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
    27901 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
    27961 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
    28021 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
    28081 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
    28141 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
    28201 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
    28261 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
    28321 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
    28381 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
    28441 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
    28501 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
    28561 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
    28621 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
    28681 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
    28741 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
    28801 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
    28861 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
    28921 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
    28981 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
    29041 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
    29101 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
    29161 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
    29221 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
    29281 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
    29341 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
    29401 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
    29461 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
    29521 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
    29581 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
    29641 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
    29701 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
    29761 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
    29821 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
    29881 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
    29941 tatatgcagc ttatgagcat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
    30001 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
    30061 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
    30121 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
    30181 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
    30241 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
    30301 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
    30361 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
    30421 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
    30481 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
    30541 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
    30601 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
    30661 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
    30721 gtgaatggt
//
LOCUS       DACEOE010000011        30475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEOE010000011 DACEOE010000000
VERSION     DACEOE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.011"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            146..1015
                     /locus_tag="IW774_000475"
     CDS             146..1015
                     /locus_tag="IW774_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1152853.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1224..2249
                     /locus_tag="IW774_000476"
     CDS             1224..2249
                     /locus_tag="IW774_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1152854.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2288..3238
                     /gene="manA"
                     /locus_tag="IW774_000477"
     CDS             2288..3238
                     /gene="manA"
                     /locus_tag="IW774_000477"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1152855.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3332..5035
                     /locus_tag="IW774_000478"
     CDS             3332..5035
                     /locus_tag="IW774_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1152856.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGEIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5037..7070
                     /locus_tag="IW774_000479"
     CDS             5037..7070
                     /locus_tag="IW774_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1152857.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7081..7521
                     /locus_tag="IW774_000480"
     CDS             7081..7521
                     /locus_tag="IW774_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1152858.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7721..8848)
                     /locus_tag="IW774_000481"
     CDS             complement(7721..8848)
                     /locus_tag="IW774_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1152859.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8851..9528)
                     /locus_tag="IW774_000482"
     CDS             complement(8851..9528)
                     /locus_tag="IW774_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1152860.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9792..11558
                     /locus_tag="IW774_000483"
     CDS             9792..11558
                     /locus_tag="IW774_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1152861.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11573..12010
                     /locus_tag="IW774_000484"
     CDS             11573..12010
                     /locus_tag="IW774_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1152862.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12023..13174
                     /locus_tag="IW774_000485"
     CDS             12023..13174
                     /locus_tag="IW774_000485"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1152863.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13236..13934)
                     /locus_tag="IW774_000486"
     CDS             complement(13236..13934)
                     /locus_tag="IW774_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1152864.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13927..15279)
                     /locus_tag="IW774_000487"
     CDS             complement(13927..15279)
                     /locus_tag="IW774_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1152865.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15502..16902)
                     /locus_tag="IW774_000488"
     CDS             complement(15502..16902)
                     /locus_tag="IW774_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1152866.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17063..17800
                     /locus_tag="IW774_000489"
     CDS             17063..17800
                     /locus_tag="IW774_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1152867.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17925..18404
                     /locus_tag="IW774_000490"
     CDS             17925..18404
                     /locus_tag="IW774_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1152868.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18401..19168
                     /locus_tag="IW774_000491"
     CDS             18401..19168
                     /locus_tag="IW774_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152869.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19398..19967
                     /gene="lepB"
                     /locus_tag="IW774_000492"
     CDS             19398..19967
                     /gene="lepB"
                     /locus_tag="IW774_000492"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1152870.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20071..20673)
                     /locus_tag="IW774_000493"
     CDS             complement(20071..20673)
                     /locus_tag="IW774_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1152871.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20779..21248
                     /locus_tag="IW774_000494"
                     /pseudo
     CDS             20779..21248
                     /locus_tag="IW774_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21354..21938
                     /locus_tag="IW774_000495"
     CDS             21354..21938
                     /locus_tag="IW774_000495"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1152872.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21941..23014
                     /gene="prfA"
                     /locus_tag="IW774_000496"
     CDS             21941..23014
                     /gene="prfA"
                     /locus_tag="IW774_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1152873.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23007..23846
                     /gene="prmC"
                     /locus_tag="IW774_000497"
     CDS             23007..23846
                     /gene="prmC"
                     /locus_tag="IW774_000497"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1152874.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23839..24018
                     /locus_tag="IW774_000498"
     CDS             23839..24018
                     /locus_tag="IW774_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152875.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24040..25065
                     /locus_tag="IW774_000499"
     CDS             24040..25065
                     /locus_tag="IW774_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1152876.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25240..26484
                     /locus_tag="IW774_000500"
     CDS             25240..26484
                     /locus_tag="IW774_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1152877.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26637..27584
                     /locus_tag="IW774_000501"
     CDS             26637..27584
                     /locus_tag="IW774_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1152878.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27718..28347
                     /gene="upp"
                     /locus_tag="IW774_000502"
     CDS             27718..28347
                     /gene="upp"
                     /locus_tag="IW774_000502"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1152879.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28486..28728)
                     /locus_tag="IW774_000503"
     CDS             complement(28486..28728)
                     /locus_tag="IW774_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152880.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28849..28921
                     /locus_tag="IW774_000504"
     tRNA            28849..28921
                     /locus_tag="IW774_000504"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28882..28884,aa:Thr,seq:cgt)
     gene            complement(29040..29678)
                     /locus_tag="IW774_000505"
     CDS             complement(29040..29678)
                     /locus_tag="IW774_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1152881.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29801..30433)
                     /locus_tag="IW774_000506"
     CDS             complement(29801..30433)
                     /locus_tag="IW774_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1152882.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        10375 a         5357 c         6156 g         8587 t
ORIGIN      
        1 ttataagagt ttttactcat taatttacaa aaaacaagca aaatgcgaat tttctcacat
       61 tttttttaac aaaatcaaag ttattgcttg aaagaagaag aatccatcgt taaaataaag
      121 cttgacgtag aggggagcaa gaaatatgaa agattttcaa ttgggggctg ccacctattt
      181 aaaaagtgta gccattcggg tgaaagaccg agataaaatg attgattttt acaagaacgt
      241 tgttggcttt gatttgaaaa gagaagaaaa cgaattagcc attttaggaa cagaagaagc
      301 tggaagtgaa atgttgctgc tggaagaaag tcctagagcg aatgatcaca tgggggtgat
      361 caaaaagctg aaccgttttt cattgattat ccctagcaca gaagaaatgg cagatatcct
      421 ttgtcgtatc cgtaaaatga attatccaat caaagaagct ttagaagatc atggacgtat
      481 ggggattttg ctcgtagacc cagaagagaa tgaattggaa atttaccatg aagagaataa
      541 gaacgaccaa agagaaattc cagaaacaat ggatcaagaa gcattattaa gaaaatctga
      601 agaaaaattc agtaaactat caacaggttc ttattttgaa aaaatccatt taaatgttac
      661 taacttaaca aaagaacgcc aatttcttaa tcatgtactt ggtttgaagg tacataatga
      721 agaagagggc atccatgtat tgaatgaagg agatttccat gttggtttga cagaagctag
      781 cggaggcgca attgatttgc ctacagatga agtgattgga cttgattttc tgaaattcat
      841 gatcgatact gaaagcatgg aaggactgat tgctcacttg cagcaactat cagttgattt
      901 cttcttagac aaaaagcaca cagtactcac aatctatgat gctataggaa tcgaatggtg
      961 gttcgtcagt gagccaaaag aaaagaataa aactgacatt cgatgcgacg cttaactttt
     1021 tctttcttaa cgattgtcgc atgtctcgtc attttatgaa cggtcatact atcgcttatt
     1081 ctagagatag aaagagaaaa tagtataccg ggggaaactg cgcggttttt cgcgcagttt
     1141 ttttgtgtaa aaaaggttga ttcctcatag attaagcttt aagcttggac tgtaagaatc
     1201 tgatagaaaa gcaggagaaa aagatgtatc cacacacgaa acagcttatt ttgaaaaaga
     1261 tggaagaagg attttatcca ggtgtagcag tcgccttcat aaaagaaaat caagtagaaa
     1321 catttacagc cggctttgcc caagtcgttc ctgaaaagca gctgatgaca gaagatctgt
     1381 tgtttgatgc agcctcactg acgaaagtcg ttgcaaccac ttcattagtt ttacgtctat
     1441 tagaagatgg aaagatagaa ctcgatcaac cgcttcataa atatcttcct gaatttgaaa
     1501 atcagacaat cactttgcgt catttactga cacatacttc tgatatcaaa acatggatac
     1561 caaacagaga taagctatct caggaagaat tgatcgccgc ttatttgaag ctagaaccag
     1621 gtgagcaact aggaaaagaa gtaaaatata cagatgccgg tacgattctt cttgggttga
     1681 tgctagagga aatttaccaa gaaactgcag tggaaatctt tcaagaacaa gtattggaac
     1741 cattaggaat gatgaacagc ttatttcttc cttttccttc caaaaaaatc gttcccacag
     1801 aacaactgcc ttcgggtgag gttttgcgtg gacaaactca tgatccgaaa gcaagagttt
     1861 tggctgaaca tgcaggtaac gcgggtctct ttattgattt gaaagacagc attacttttg
     1921 tccaaatgta tttgaacaag ggaaaaatcc gtaatggtcg ttttttagaa gaagagacga
     1981 ttgattcttt actgcaagat caaacaccga aaaaaaatgg ctatcgttct ttaggatggg
     2041 acttaaaaaa ggatgtagaa gacggtcatg cgattctttt tcatactggt tatacaggta
     2101 cgtttttctt gattgatata aaaacacaag aagcgttgat atttttgtca aatcgcgtac
     2161 atcctgtcga tcatcgtgcg gagtatctca tatgtcgaga tgaaattgtt gaaaactttt
     2221 tgaaagagaa agcggtatta ccgaaataat gctataatag ctatgaaaaa tgagaggagt
     2281 ttgaattatg caggaaccta tgtttttaaa accagttttt caagaaaaaa tttggggagg
     2341 aagtcgttta cgttcagtct ttggttttga tatccctaac gataaaatcg gagaagattg
     2401 ggcgatcagt gcgcatcctc atggtgtcag tgtagtagaa aatggcgaat tcaaagggaa
     2461 aagattagat gaactatggt cagaacacaa ggaattgttt ggccatcctt cagaaccagt
     2521 gtttccatta ttgatcaaga tcttagacgc agaagatgat ctatctgtac aagtacatcc
     2581 agatgatgcc tatgggatga aacatgaagg tgaattagga aaaactgaat gctggtatat
     2641 catcgatgct gaacctggtg cagaaatcat ctatggtcat catgcaaaga caagagaaga
     2701 actagctgag atgatcaaag atgggcgctg ggatgacctt ttgaagaaag taccagttaa
     2761 aaaaggtgat ttcttttatg taccaagcgg aacgatccat gctattggta aaggaatcat
     2821 gattttagaa acacagcaaa gttctgacac gacttaccgc gtgtatgact acgacagaaa
     2881 agatgcgcaa ggacaaacaa gagagctgca tatccaacaa tcgatcgatg taacaactgt
     2941 ccctgcgaaa acaccagaat tacaaatcaa agaagtccga aaagggaatt catcgatcgt
     3001 cacttattta gaaactgagt ttttcaatgt atatgagtgg gatattaaag gaatcacatc
     3061 tttcaaaaaa caagcccctt atacattgat gacggtaatc gaaggagcag gagaccttgt
     3121 cgttgacgga aaaacatatc cattagaaaa aggaacaagc tgtattattc caaatggcgt
     3181 ttctgagtgg gcggtccaag gtgaactagc aatcattgcg tctgaaccag gcaaatagtt
     3241 cccagatagc tgaaagcagc agacggtttt tcgtttgctg ctttttctac ataaaatccg
     3301 tccatcaata aatagaagtc tggtgaaaaa gatggaatac aaatggaaag caaacaacag
     3361 cagtcgcgtg tctatgaaac aactgagaaa agctggacat cacctaaatg aaatgatcat
     3421 gccaaatcta agtctgttta tcgcttgggg aatcttgtca ttagcaacaa aaggtgtgtc
     3481 tggcgatttg cacaccacat tagaagaggt atgccattgg atgatccagc tgcttttacc
     3541 catcttgatc agttattcag gcgggaaatc ggtaggggga caaaaagggg cagtcgccgg
     3601 tgaaattgct tctttagggt tgatcgctcg ttccaatgct ccacaaatcg taggagcgat
     3661 gattatcggg ccgcttgccg gcatcgtata tgaacaattt gtttctagat tccatgtgaa
     3721 attcaaggct ggatatgaaa tgcttgtcag caacctacta gtaggactga ctggtagcgt
     3781 tatttgtata attggaatcg tttttattga accagtttta aatagtgttc acttgttgct
     3841 cgcctccgtt gtgtcttggc tagtcacaat gaatctgtta ccacttgtca gtctgatctt
     3901 agaacctcta aaagtactat ttttcaataa tgcaatcaat catggtgtat tgacaccttt
     3961 aggaatcgaa ttctcacaaa cagtcgggta ctcttcctta tttttgatgg aggcaaatcc
     4021 tggacctggt ttagggatct tactttctat cctttgtttt gcagaaaaac aggaaaaagt
     4081 aaatgcgagt ggagcattga tgatccatgc gattggcggg attcacgaaa tctattttcc
     4141 atttgtcttg ctgcgaccgt atctattttt agctgtaatg gctggtgggg caagcggcac
     4201 gttgatattt caatggttag atacgggatt agctgcacct gtatcgcctg ggtcactact
     4261 cgtgattttt gccaatgcgc cttatccaca actagctggg gtaagtatag ggatagcaat
     4321 cagtacattt gtgaccttca tttgtgcagc gatactattg aaattaccaa ataaagaaaa
     4381 agagaacaaa acggtcaaag aaaggggaga aatcttggaa ccatctatca ttcattccgt
     4441 catttttgct tgcgactcag gtatgggatc aagtgctatg ggagccgccc tgctgaaaaa
     4501 gaaattagca gcagaaaaaa tcgatctttc tgtcggatac agctctatct atgaattgaa
     4561 taaccaagta aatcagctgg ttatcgtgca acatgaacta gcgggtcttg caagagaaaa
     4621 agtacctcaa agtcaaatct atgtactgga tcactttttg gaaacagata tcttgctgtc
     4681 acgaataaaa gaagcgatcc aatttccaaa gcgggaagaa aaaaaagaga tagcagatat
     4741 cacagaacaa aaaacgtcgc ctgctaatat tgtatttttg tataaaaaca acagacgcgg
     4801 ctcacaaact atgggcatga cgattttaag tcaattagca aaaaaagcga aaaaacagtt
     4861 tgttatctca aaagaatcaa tagaaaaaat caaaatccaa gaagaaacga tttatatcgc
     4921 acaaagagaa ctagctgtaa ctgaacagct agaagaaaag atccctcggc tagttctagt
     4981 tgaacattgg gtagctacga atgaatacga aaaactcgtg aagggagaaa tataagatgt
     5041 atttatcgcc cagagaaaaa caattattag cagaatttct cagtagcccg tcacctgtat
     5101 ctatccaaaa aatgatgaat ttattgaagg tcagtaaacg gactatttac cgcgaattag
     5161 ataatcttga agtctcattg aaatcggtaa atgcttcttt gaaaaaagca gctagaggct
     5221 catttgtcat tgaggcatca gaagaagtat tagagcaatt aaaagaaaat acagatggga
     5281 aaagtaatga cttatccact gctaaacgac aacacgcgat tttagccgag ttgttactga
     5341 cgacagagcc gttgagtctt gctcatttca tggaatcata tcgcatcagt aataccactt
     5401 tttatgcaga tatcaaacag ttggaagaaa gtattcgcca actgcctttg gaaatcattc
     5461 ggaaccaggg atacgagatc gaaggaccgg aaaaataccg ccggcttctg acagcgaatg
     5521 tattagaatt agaaatcaat gaatatgaac tttttcatag tatttctttc gatgcatcac
     5581 tgaattattt ttttcgattc gttgatccgc aacatctatc tcttgcacgc aaagtaatcg
     5641 gggaagaatt gatccaaaaa aagaccaatc taagtgatag aaagcttgaa catttggttt
     5701 tgatgctgac attgacgatg gagcgtgtga cgaaaaatca tttactgaca aatgagacat
     5761 ataatagtct ggcaaacaaa gaattactac agacagctaa acgattgttt tcacggatcg
     5821 cttctgagac aaaacaactt tatccagtaa atgagatcgt cttttttgca ggattattaa
     5881 atgacttcac gaactcattt gatcagaatt tctttgaaga gacgttcgat acacagcttg
     5941 cttatttagt aaaacaactg attcaagaag taagtgaaga gacgaatgtc catttttatg
     6001 aagatgaaac tttgtacaaa atgctgttga ctcatttatc tggtgtgttt tcaagagccg
     6061 ttttacaaga agagacactg accaatccga ttttagaaaa aatcatggtt caatacgagg
     6121 aagttgcgaa tgcaatccgt aaagcagtag agcggatttt tcaagaaaaa aaattatccg
     6181 aagaagagat cgcttatatg gtccttcatt ttgcgaattc attggaacga agtccgaaag
     6241 aaagcaatat cagtattgca ggattttcgc caagtggatt agcttctact agcatgttgg
     6301 aactacgatt gaaaaggtat tttcccttca tccaacaaat tcatttctat cggattgctg
     6361 atttaaaaaa aatcgattta caagaagaat atgatgttgt tatttctaca tctattttac
     6421 caggatacgg tggaaaatat attttagtct ccccgctact gctagaagat gaagtcaagc
     6481 agctaaaaga agctttttcg catattgaga agaaaaaaag gcaaaaaatt acgtataaca
     6541 gcaataacga gtggatgata gaagatactt atgaggaaac aatgcatttc atggatcaaa
     6601 tcaatcaatt actcggtcgt ttttttatcc aagaactgga taatcctgag acgatttccg
     6661 aaacgattcg cagcttattt ccttattttc ctgaaggact agtaacggat gaagaggtgg
     6721 ttcatcgccg gctgcttaag cgatatgaac aagcacctat cggtattcct catacaaata
     6781 tggggttatt ccatacatcc agctcattga tttctaaacc gatcttttgt attttcaatt
     6841 tgaaacatcc gttgaccatt gaagggatgg atagacagcc agtcgatttg tcccgtatgt
     6901 tagttatgct ggcgccttcg ccaatccaag aaaaagacgg gattctttta ggaaagatca
     6961 gtggagcaat catcatgaat gacttgaaca ccgagatctt ccgctcagga aataaagaaa
     7021 tcgtttatca gttgttagcg aaaatcctga ttgaagaaat aaagaaatga aggtgaaaag
     7081 atgatacagc taccagcaga aaaaatattg ctcgatcaat cctatcaaaa ttggaaagaa
     7141 gcgttgaccg agatcggcca gtggatgacc caacaagggg gaatcaaacc ggcatatatc
     7201 caatcaatga tcgaaagaca agaaaaagcg agtgtctata tcgggaattt cgtagccttg
     7261 ccacatggga ctgacaaaga tgaagaagta gtagaagaag gaatctattt agtccaagtg
     7321 cctgatggtg tggactttgg aacaaaagag gaaccaaaaa tcgcgacact cttatttgta
     7381 gtggctttaa aaaaagaaca tcagttagcg gtgctccaag atttagcctt tttctgttca
     7441 gacatcgacc aagtaatggc gctcagtgat gcggaaacca ttgaagaagt ccaaatgatt
     7501 tttgaacatg gacatatata accaatgttc acagtaaaaa aagctgcctt gcatcaagta
     7561 ataacttcaa ccaagataaa aataagagga gctatttttg ttgggaggtt cttattactg
     7621 cggatgcaag cttttgaaaa ctatttattc tagaaaaccg tttatttgaa ataggggacg
     7681 gtgacaaggt ttttgtcacc gcccctatta tttttgtgtt ttaatagctc ttttttatat
     7741 agatagtgcc catacctatg aataataaaa cgatactcca tataccaagt gtcaagatac
     7801 cattcatgaa agtaacctgt tctgaagcta gtggcgagaa aatatctcct cctatgatca
     7861 cattttgaaa atcaacataa gaagttggca agtaatgact gattgattct atccatccaa
     7921 tcgaagaaaa caagcgatac tctgctaaga aaagacaaag aagtgtacct aaaagattga
     7981 ctagagaatg ttcaaataaa gcagatagaa gaaaactgat tgtacttaaa aagagcaccc
     8041 acaacaacag gaacagaaag ctctggaaaa gatactcata catcggcagg attcgtatct
     8101 cctgaccaat gatcgttcct acaggataac ggaagtctcc caaaccattt ttgattatta
     8161 ctagtataga gacgatcaaa aatggcatca cgagacttag catagctgca ccataataaa
     8221 tggtaaaacg gatgctatgt ttctttacca tactatccgg taatagattg actagagaga
     8281 ttacctgctt tcttttttca taagagatga tggaagaaag aaacaaggcg gttatacata
     8341 aaatcaaaaa actagaaatc attcctgaaa aaatattttc gtagtaattt gctaaagaga
     8401 gatgcgctgg caagtcaaaa atagaatatc tttggatctg atgttctttc atataacgga
     8461 gcaactcgac acgtttttgt tgttcaatga ttggaattcc ttgtctttga ccagaaatca
     8521 gtcgatccag tatatctttt tcataatgat aagttgcttc gaccatttgc tggacattcc
     8581 ctgtgttttt tgcatgtaag attgcttcga tcagtttgtt gcttttctct aaactttcca
     8641 caaattcttt ctcattctca tcttctttca acgtttcaat caaacgttgc atctcttcat
     8701 agttatgatt cagttcatcg aattctgtat aagcaagttt atgatcaaaa aaaagtggga
     8761 atgcaaagag cacgaccaac aatagaggaa ataaaaagtt tactttgttt tttagtaaga
     8821 gattgcgttc aaatgtaagt aaagacatag ttacacctca tataatttac gataaatagc
     8881 taaaatctct tccatatttg aggattctgt gacgatttct ccatttttta gaaaacatgc
     8941 tttagaacaa atcgattgga tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc
     9001 ctctttctgc cattgtctga tgaggctatt caagatatca atacttcctg ggtctaatcc
     9061 gttcaacggt tcatcgaata acatgacaga tgaatcactg atcaagtaag cagcaagcaa
     9121 gacatgttgt ttcattccta gagacatttt tccaatcggc aaagtaaggt actcagtcat
     9181 cttcaatcgt tggaaaacag tttggcattc tactttgctc tgccaatagt atttgatcaa
     9241 agtcaaataa tcaacagccg atagatagtc ggataactga ttcgttgatt ctagaaaaaa
     9301 gaaagaccgg cgataatctg tataagcagt aggagagatg ttgtgaaggg acaattgatc
     9361 ataagttgtt ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc caaatccgtt
     9421 cggggcaatc aaagcagcta cttctcctga ttctactaaa agattcaatt gtcgaatcac
     9481 acgtttttta ccacgtttca tgttcatgtt tttgatcagt aatgtcatag ataggcctcc
     9541 aatataaaat ttaataacat aatagaatgg aaaacatcta aattaaatgc taacaaaagt
     9601 tattgtttgc atattataaa aaaattaaaa taacagtttt atttaaaatt gaagttttct
     9661 gataccgaaa tctttttatt ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc
     9721 aaagtacgac tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag
     9781 ggagagacaa aatggaacgg acgattccca aaaacaacat ttcgctaaaa gcgagggtac
     9841 aaaaattagg cagtacttta tcaagcatgg tcatgccaaa tattggagca ctgattgcct
     9901 ggggtgtatt aacggctcta tttatcccag atggttattt accaaatgag gctttcgcta
     9961 ccatggtaag cccaatgcta acttatctga ttcctttatt gatcggttat actggcggaa
    10021 aagtaattgc tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg
    10081 tcggaacaga tattccaatg atgctaggcg cgatgatcat gggacctctt ggtggatatg
    10141 ccatcaagaa atttgatcag ctattccaaa aacggatcaa atccggtttt gaaatgctag
    10201 tcaataattt ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga
    10261 ttggtccagt tgtcgatgca ttgactcaag caatggcaaa aggtgtagag atcattttat
    10321 ctgcccattt gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga
    10381 ataatgcaat caatcatggg attttgacac cattgggaac tgaacaagta gcagaggctg
    10441 gaaaatctat tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg
    10501 cttttacttt gtttggcaaa ggagcagcaa aatcttcagc accaggagcc atgatcattc
    10561 actttttagg cgggatccat gaaatctatt ttccttacgt tatgatgaaa ccgttattat
    10621 tcttatcagt tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg
    10681 gactaagagc gccagcttct ccagggtcga tcattgcgat tctagcgatg acaccgttca
    10741 attcttacct tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg tttggtattt
    10801 ctgcagtaat tttgaaagcg gatgcgaaag atacaaatga agacttcgaa aaatctgttc
    10861 aagagacaaa ggcagctaag caagcttcca aaggtgccca aggtattcct caaggagcta
    10921 cattctctgg cgtacaaaaa atcatttttg cttgtgatgc aggaatggga tcaagtgcga
    10981 tgggcgcttc gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacca
    11041 atcaagcaat cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac
    11101 tacaagaaag agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt
    11161 tgaattcacc tcgatatgat gaaatcattc aagaattaag cagcaagcag aaaacatcag
    11221 tttctgaaat tgagaatgag ccagcagaag agtttctttc agcaaacatc aaacaaatca
    11281 aagagattct acttgtccac gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc
    11341 tcaaagatat attagacaag aaccatttaa ctatcccgtt aagaaaagtc aatatccaag
    11401 atttaaaaga tgaacctgat tcactgatcg tagccaaaga aagtttagcg gaaaaagtac
    11461 agatggctgc acctcaatcc attcatttat cagtcaacag catggtcaat ccgcaaaaat
    11521 acgaatcgat tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag
    11581 tttgccattc gacatgatcg agctcaatcg atcttttaaa accaaagaag aagcgatccg
    11641 ctattgcggt cagatgcttg tagaagcagg gtgcgttgat gaggaatata tcgatgccat
    11701 ggtagataga gacaaaatgt tatctgttta tatggggaat tttatcgcga ttcctcatgg
    11761 aacagatgaa gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga
    11821 tggtgtagat tttggtaccg aagaagaaga aaagattgcg actgttttgt tcggtattgc
    11881 aggagtcgga gatgagcatt tgcaacttgt acagcaaatc gcactttatt gcagtgatat
    11941 ggacaatgtt gtccagctag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc
    12001 aattgcttaa ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg
    12061 aggatttatt ggagaggttt tggcagctaa tggatttgag attactttcg tagatgtgaa
    12121 tcacacgatc attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga
    12181 atcaaaagaa aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa caaatccaga
    12241 aaaagtaatc caagaaatcg ctgaagcaga tttagttact accgccattg gcccgaatat
    12301 cttacctttt attgctgagt tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag
    12361 cacaacatct ttagatatca ttgcctgtga aaatatgatt ggcggttcag catttctaga
    12421 aaaagaagtg tataaatatc ttccggaaac atcatttact gacaaatata tcggctttcc
    12481 aaatgcagca gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca
    12541 agtggaacct ttcaaagaat gggtcattga cgatagccaa agaaaaaata aaaaaatcca
    12601 attaaaaggg gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag
    12661 tgtcaatact ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat
    12721 tgatgaagca atgcaagatg cgttagttgt ggcacaatta aaatcagttt tgcaggaaac
    12781 aggaagtttg ctgattgcca aatgggggtt tgatgcagag cagcatcatg cgtatattga
    12841 aaaaatcatt catcgcttcc aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg
    12901 gacaccatta cgtaagttag gttatcaaga aagatttaca cgtccagtga gagaactaca
    12961 agaacataac ttgacttgcc cacatttgac ggcaacaatg gggattattt ttaattacta
    13021 tgatcctgaa gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg aaaacttgga
    13081 acaattgatt caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca
    13141 aaacgtgaac cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg
    13201 acgaaatttt tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag
    13261 ggcagatcca attctttacc gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc
    13321 aggttctcat ttcttgcaag gataggtcca tggaaataag aaccgaagac atttcggtaa
    13381 atgacacctt cagattggtc ttcaccatta tttccttgac cttgtaccac tttccccaaa
    13441 ggtcgttcac cttcacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt
    13501 tcattgaatt cttcgttatg gataacgata tcaccgataa accggttatt ttcctgactt
    13561 aacgtgtagt gatccagtgc actgattcct tggatctttt ctccattagc tcccatgtag
    13621 tagtgaccta gcaattggta gccaccacaa atggctaaca tgacgccatc attttcgata
    13681 tagttgatca aagaatcttt ctttttctgg atgtcttttg aaatgattaa ttgctcaaag
    13741 tcttgaccac cgccaaagaa aaccaagtca tatttatcag catcaaaagg ttcatgtata
    13801 ctaacgatct ccgtatggca agaaaccccc attttttctg ctatatattt caacatcaaa
    13861 aggtttccgt tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt
    13921 tcataattag ccacggttca ttcctccttt gataaatcct tgagcagcta gttctttacg
    13981 aagctgcaat acagcagtgt aagtagctaa aatatagaca tgctcagttg gcaattcttt
    14041 gattttttcg attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc
    14101 ggcaactttt agacgtaaag tcatatctgt atgacggtct cctccagaaa taacagctgg
    14161 gatatccata tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc
    14221 gtctgcgtaa ttggcattta ataaagccac taaagaaaat ggataaggag ctagaccaat
    14281 catatcgatt acttgattga tacctacagg atttttgaca agtactaatg tacatttttt
    14341 cccattgata ttgatgactt cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg
    14401 tttgatttta tcaggatcga cattgaagta ctctgccact gcagtagctg ctaaagcatt
    14461 gtagacatta tacataccgc caacttcgat tccgtattca tgaccatcga tcacgaaatc
    14521 tgctgacaca ttgtccatcc gtaccatatc agttaatcga taatccaatt caggacgttt
    14581 gaagtcacaa tttggacagt aatatttccc taagtttgca taagtgatca ttttataatg
    14641 caagatgtga tgacaatttg gacataagac accgtctgta ttgtaatgag ccatttgttc
    14701 acgatcttct tcatggtcaa agccataata ttttctagga ttgactgttt cgatagaatt
    14761 gaagattggt gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc
    14821 tacaatcatt ttataggtcg tatagatttc tccataccga tccatttgat cacggaaaat
    14881 atttgtaaat aagaacagtt ctggtttcat atattctgtg actttgctca aacttgcttc
    14941 atctatttct aggacagcaa actttttctg tccttttttt ggttttgcct gcaagaaagt
    15001 cgatacaatt ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg
    15061 ctggcgcaaa atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac
    15121 aacgacgact tggtagtctt ttgccaaact atctagtata tgtggatcga ctttcagcgc
    15181 tagtttacct ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt
    15241 ttttccaact gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat
    15301 ttgctgacga ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt
    15361 tcttattctt aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa atcgcatgat
    15421 ttaacaaaaa aacagcttga aacaaaatga ttgtttcaag ctgctgtgat tttattattc
    15481 cctcttacct atattcccat tttaaccgat aataattttc tcgcttggat attcatagcc
    15541 tagatctcgt gtttcttttg gcacgaataa catcaatgta tatagcattc cgatccgccc
    15601 aataaacatc agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc cggtaatccc
    15661 taaagataaa ccagttgttc caaacgcaga agttacttcg atgatgatcg agatcagcgt
    15721 ttgttcttct gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat
    15781 aaagacaacg actgattttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat
    15841 gttatcttca cttttgagga aggaatatag atacaaaccg atgattgcta cagtagttgt
    15901 tcgaatcccg ccgcctacag aactaggact gcatccgata aacatcagta aagaaaaaat
    15961 gatcagtgtt gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag cattacgagt
    16021 cgtaatcgaa taaaacatcg aattgatcca tttgacactc atattggaat cttggaataa
    16081 atggtccttt tccaatagat aaatcaagac agttcccgaa acaaacaaga taacaaaagc
    16141 taacacagct aatttggtaa acaaggaaaa acgaaacgga agcttcgcat tggaacgttt
    16201 gtataatagc cattcccggc actccatcaa tacaggaaaa ccaattcctc cgacaaaaat
    16261 caaaaacatg atcaggatca agaaaaaata gtcatgtgca aagggcttga tggaatcccc
    16321 agtgacgtca aacccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac
    16381 ggcatcacgc caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc
    16441 aaaaagtaat tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc
    16501 agacaaacgc ggttgattca tatccgtcat gatcagctgt cgctgtttca gtgtgatccg
    16561 tcgtttagaa aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg
    16621 aaataatatt tccagcagta taataccgtt atcattgaac acagaattga tatcaaaagt
    16681 cgatagtcca gtcacactca ctgtactgat tgccatgaac agcatgtcca caaaagaaac
    16741 atggctatct ggttcgcgaa aaatcggcat ataaaataga acgagtgata agaccgtcat
    16801 caatatataa taaaaaacga tgatttggat cgatgagaaa tgattggcag caaacttctg
    16861 tcctcgccgc tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca
    16921 atagtcgttt ctaccagtat acaaggggaa ttaggaatac tcaaagaaat tatcaaacag
    16981 ataacaatac agctttcgat taaaatagaa atggcttata ataaggaaaa tcctagaaaa
    17041 aagaaggagg aggaataaca aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa
    17101 tcaaaaaata aattatgata cagttctgaa aaaaatgatc atgcaatggg agaaagaaga
    17161 agtaaagcct aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacattgga
    17221 attcatgtct caatatgcag agatcacgtt atttttttca aattcgaata ttcatcctaa
    17281 aagtgagtat ttgcgaagat tacatgaaca aaaacgattt gtagaccaat ttaatgagaa
    17341 aactgaccat catgttgcgc taatcgttga tgaataccgt ccaaatgaat tcaccaaaat
    17401 ggttttaaca aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt
    17461 caatatgcga ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg
    17521 aagtgccctg accatctcgc caaagaaaaa tgcagctttg atcaatcaga tcggaatgga
    17581 tatccaaaag atttacggga cacaatactt gccaagtgat ttcaagaaaa acaaaggcta
    17641 tgaacgctct ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt attgtggttg
    17701 cgtgttcgct gccaagcagc aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc
    17761 ttacttaaaa acatcaaccg tagtttttga ggaaaattaa agaaatcttt cggaaaaacg
    17821 taagaaaaaa ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt
    17881 aaagtaagga aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac
    17941 ctattttgaa gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta
    18001 ttgtgaatag ttccagctac cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa
    18061 caccaactac ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca
    18121 ctacgacaag tacatgggac gatacaacaa ctagttcgtc gtatgatgag acatatacaa
    18181 ctgacagctc aagcgaaaca agtaatacgg taccagatgg cactccatcg gattcaacca
    18241 atccaagtac aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg
    18301 tagatgataa cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag
    18361 gaaataccca aacgtataac cagcaagcag gtgattctca atgacatttt tactagacct
    18421 tatcaacggg gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg
    18481 tggatatacg attttaagtg tgattcccac gctttattta ttgagaattg tttatggact
    18541 ttggcaaaac caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt
    18601 ttattttact gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt
    18661 cactcagctt tttgtgaagt atttacctga tgaagcgttc aacatccaga cagaagaatc
    18721 gatagataat ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga
    18781 cgaagattat caattgaaat tagccgagaa tatgcggtat ttgattggaa atggtgagat
    18841 caaaacaaat gaactcggtc gcatggatgg atttctggtc gatagaaata cattgtatcc
    18901 ttattactat gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta
    18961 ctcggacatg gaaaaagtag ggaccattcg acttgactca acagaagaca cattggaacc
    19021 tgtcggattg ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct atcatgagcc
    19081 ttatcgtctg aaattgattg caaaaaaggc acagtcgcag gaagaaccag tggtttatag
    19141 tcgtagccaa agaagaagac attcataatg tgatgaaacg ccgtgtatca gtggttgcga
    19201 taaaaacgtt tattcggaat aagggggatg tgacacgtct tttgtcacgt tcctttttta
    19261 tataacgaga aagataagac gaacacatca cttattcttt cctttcattt ataaacattg
    19321 tagaataaag aaagtacaac gaagtattgt gaaaaaacgt aataaataac gaggaaaata
    19381 caagtaggag gtaagctgtg aaagaatttt tgaaaaattg ggggattttg atccttgtat
    19441 tagcggctat tttattagct agagtgtacg tgtttacacc agttactgta aacgggcatt
    19501 cgatggaccc tacattaagt gatggacaac gattgatttc atctaagatc tcaaactatg
    19561 aacgaatgga tattattact acaaaggaac cgggggatga agaacggatg attgttaaac
    19621 ggatcatcgg tatgcctgga gatacagtca agatggaaaa tgaccagttg accattaatg
    19681 gcaaaaagta tgatgaacct tatctggatg agttcaaaaa agaattttca gaagataagt
    19741 tacaaggaga atatgcttat agctcaggat tccaagcaca agcagaaagt tcatccacct
    19801 ttacgagtga ctttgaatat actgtgccaa aaggaaagta tcttgttcta ggcgataacc
    19861 gcttgatttc aaaagacagc cgaatgtttg ggttagtcga caaagatatg atccaaggaa
    19921 aagttgtttt ccgttattgg cctctttcag aaattaaaat catgtgatga aaaaataaaa
    19981 agaagctgtg acatgttttt tgtcacagct tctttttccg aaaattcctt cttatttctt
    20041 ggagttgctc actttttttg caatctcatt ttaagctttt tggctgatgg ataagaaaag
    20101 cgaagcgttt ttgttcataa atagcttttc cccacgatgg aaggtatgac tagtagcaga
    20161 gacaaagccc gttttttcca atcgattatt caattcttct tggctaaagc cattatggac
    20221 tttcggatga ctaatttgtt cattcttgtc aaagtctacg atgatcaatt gaccgccagg
    20281 agctaaaatt tcataaagtt ttgtaagaat attctccgta tcaggaatgt gtaaaagtac
    20341 aagtgataga aggatgagat tagcttggac agcaggaaat tcgacagaag catcagcgtg
    20401 gatcgttcgg acatttttca gatctgctgc ttggatcttt tcttctgcca ttttcagcat
    20461 agtttctgaa gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg gtaaactaac
    20521 aagcccagtt ccgcctccgt aatccagcag gactttttgg tggctgtcgc gcggtagata
    20581 agtagttagt tcttcgcgaa taatcttggc taatgcttga cggtcagggg aatcataatg
    20641 gttagcgatg gaatcaaaaa tattttttgt catcaaatag ctccttacgt aaaactttga
    20701 aattttaact tattatagca cgtgaaaaga aagaaagcat gctatactca attcaaaaag
    20761 ggagaatcta gaaagaatat gccgatatcg tttgtaaaag atagagagga aaaaggaaag
    20821 tgtgtccgag aaatattact agacttgcct gagtggtttg ggcttcctga atcaacggaa
    20881 aaatatatcg aggaatcaag taaactacct ttatggtgtg aaaaaagaaa agaagaatat
    20941 ttaggcttta tcactctttc acagacaagt gaagatactg ccgaaatcta ttctattgta
    21001 tgggaataaa aaaagactat catcaccaag gattaggaac aaaacttttt aaagaagcag
    21061 aaggttacgc agcaaaacat tacaaatatt tgcaagtgaa gactgtagac gaggggcact
    21121 actctatata tgatcaaact atttgtttct acgaatcatt aggattttca aggttggaag
    21181 tttttccaaa tttatgggat gaatggaacc catgtttagt tcttgtcaaa aaattggaac
    21241 aaaagtgagc aatagattta atactaatct gtgcttgatg aaaattctca ctgtaaaaaa
    21301 tgctgaaaat caagtataat accataggaa caaactggaa ggtgaaaaag agcatggcac
    21361 aactattttt taagtatggc gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg
    21421 cacataatta tgaagaacaa aataagccag ttgtcttaat gacaagcggt attgatacaa
    21481 gagatggttt aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg atgccgatat
    21541 ttgaagaaac agacgtattt gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc
    21601 tgatcgatga agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg
    21661 acgaattgga tattccggtt atggcatttg gtttgaaaaa tgattttcga aacgagttat
    21721 ttgaaggatc aaaatattta ttgctttatg cagacaaaat cgaagaaatg aaaacaatct
    21781 gttggttctg ccacaaaaaa gcaatcatga atttacatta tattgatgga aaccctgttt
    21841 atgaaggcga tcaagtgcaa attggcggaa atgaagctta ttaccccgtt tgtcgaaaac
    21901 attactttca cccatcaatg attacagaag gagaataagc atgtacgatc aattacaagc
    21961 aattgaagac cgttatgaag agcttggaga acttttaagt gacccagaag tgatttctga
    22021 tacaaagcgt tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt
    22081 ttatcgtcgt tataaagaag tagtacaagg aatcaccgat acagaagaac tgcttggtga
    22141 aaaactggat gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga
    22201 aaaagaagtc ttagaagaaa aaatcaagat cttgctttta ccaaaagatc caaatgacga
    22261 taaaaatatc atcatggaaa tccgtggagc agcaggcgga gacgaagcag cattattcgc
    22321 tggcgatctt ttcaatatgt accaaaagta tgctgaagca caaggctgga aaacagaagt
    22381 gatggaagct aacgttaccg gaatcggtgg atataaagaa gtgatcatga tgatcaccgg
    22441 agacaacgta tattcgaaat taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc
    22501 ttcaactgaa tcgcaagggc gtatccatac ttccaccgca acagttgtcg tgatgccaga
    22561 agctgaagaa gtagagatcg atattgaaga caaagatatc cgtacagata tctatcatgc
    22621 ttcaggtgct ggcggacagc acgtcaataa aacagcatct gctgtacgtt tgactcactt
    22681 accaacaggg atcgttgttg ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa
    22741 agcaatgaaa atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag cacaaagcga
    22801 gtatgatgcc aatcgaaaat cagcagtcgg aactggggat cgttcagaac ggatccggac
    22861 gtataatttc ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta tccaaaaatt
    22921 agatcagatt ttagcaggga aattagatga gatcgttgat gcattgatca tgtatgatca
    22981 aacatcaaaa ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac
    23041 gggcttcttc ttttttagag gatcatggga aagaagcata tagtatccaa tttttatttt
    23101 tggaacgaaa acaatggaaa aaacttgatt ggctccttca tatgaacgaa gagatttcag
    23161 aagaagaaca gcggttgatc gaaaccgatc tgcgacttct gcttgcagat catccacctc
    23221 agtatttgct aggatatgca gatttttatg atcatcggtt aaaggtaact gaagcaacac
    23281 tgatccctcg accagaaaca gaggagttag tcgaatggtg tttagacgag acgcctggtg
    23341 taccgttaga agtgatagat attgggacag ggactggagc aattgctatt agtttgaaag
    23401 cagcaagaaa aaattggcat atatctgctg tggatctttc agaagaagca ttagaagtag
    23461 cgaaagaaaa tgctcagaaa gaaggaacga aaatcagttt ttatcatgga gatacattag
    23521 gaccagtaat ggatcagcaa tttgatgtga ttatttctaa tccgccgtac atcagtcgga
    23581 atgaatggga actgatggat gaaagtgtcc ggtcattcga acctaaaatg gccctttttg
    23641 ctgagaacga tggattagct atttatgaaa aaatagcagt tgaagcacca agcgtactaa
    23701 aatcaaatgg aaagattttt ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa
    23761 tatttcagca agcatttcca gataaaaaag tggcgataaa aaaggatctt tttggcaatg
    23821 aacgaatgat ccaagtttat ggttgaagtt gtggatgaaa aattttttat ttgttttttt
    23881 attataaaaa aacacttatt tgacaaggat gtaaacgact ttttggttta tcaacaagtt
    23941 tatccactat tgacaaatag attatccaca aacagtggaa aacttgagga taaactcaga
    24001 caaaataaga atgtatgaaa aaggggttgt ggataaactg tggaaacgaa acgatatgat
    24061 gaaacagaat tgcaagaggc agccgaagca ctgaaagaag gagaacttgt tgcttttcct
    24121 accgaaacag tatacggatt gggcgccaac gctcttttac caaacgctgt caagaaagtg
    24181 ttttctgtaa aagggagacc gcaagataat ccattaatcg tccatgtcgc ctcttttgaa
    24241 caagtaaaag agtatgtgga caactttcat ccggcagcag agaagattgt gaagaacttc
    24301 tggcctggac cattaacttt gatatttaag atcaaaaaag acactttgcc ctcagttgtg
    24361 acgggaggat tatccacagc tgcatttcgt ataccggata acaaaaaaac actagaagta
    24421 atcgaacttt ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt
    24481 ccaacgactg cagatcatgt atatcatgat ctacaaggaa aaattaccgg gattattgat
    24541 gatggagcta cgcgaattgg tgtagaatcc actgttcttg atttaagtga tccaacagcc
    24601 atgcctatga ttttacgacc aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa
    24661 tctcctgttg ctattgatca gcatcttgta aaagaaaatg aaacaccaaa agcccctggg
    24721 atgaaataca aacactactc acccgataca cgtgttctga tggtcagaga tggcgattgg
    24781 tcaactgcag ttcaatgggc aaaaaataag aagatccgtg caggtgtcat agcaagcccc
    24841 gaaattgcgg atcaagtgcg gacagatact gcagctgttt atatgtataa cgacaactca
    24901 gtagaagcgg cggctaaagg attgtttgca ggtctaagag gattagatga accaacactt
    24961 ggattagatt tgatttttgt acaagtgtat ccagaaactg gattggggaa tgcttacatg
    25021 aatcgcttga aaaaagcagc aggacaaaat tattttgaaa aatgatcaat tggttattgt
    25081 cgcattcttt tagtatgata caatgctatc aagtaaatct tctatcagtg aacgaaaaac
    25141 taagcgtaac taatcgtaat agaagagata cgaatatttg gcaatttgtt tttgatggga
    25201 aaaataaaat tgtaggaaaa taaaaaggag ctgttgtttg tggtagatta caaaacgttt
    25261 gaccctgatc tttgggctgc gatcgccaaa gaagaagaaa gacaagaaca taatttggag
    25321 ctgattgcct ctgaaaactt tgtctccgaa gcagtgatgg ctgcacaagg aagtatcttg
    25381 acaaataaat acgcagaagg ttatccaggg catcgctatt atggtgggtg tgaatttgtc
    25441 gatatcgttg aaaatttagc gattgaccgt gcaaaagaat tattcggagc gaaatttgcg
    25501 aacgtccagc ctcactccgg ctctcaagcc aacacggctg cttatctcgc tttggtagaa
    25561 ccaggggaca cgatcttagg aatggatctt tccgcaggcg gacatctaac acatggatca
    25621 ccagtcaact tcagtggcaa aacctatcac tttgttgctt acggtgttga tccgacaaca
    25681 gaagttattg attataatgt ggtacgtatt ttagctagaa aacatcaacc aaaattgatc
    25741 gtagcaggtg ccagtgctta cggacgaacg atcgactttg cgaaattcag agaaatcgca
    25801 gatgaagtag gcgcaaaact gatggtagat atggcacata tcgcaggatt agtagcagca
    25861 ggacttcatc ctaatccagt tccttatgca gatatcacaa cgactaccac tcataaaaca
    25921 ttgcgtggcc ctcgaggcgg catgattttg acaaacgatg aagcattagc aaaaaaaata
    25981 aatagtgcgg tatttcctgg tattcaaggc ggccctctag agcatgtgat tgcgggtaaa
    26041 gcagtagcat tcaaagaagc attagatcct gcatttaaag aatatagtga acaaatcatc
    26101 gccaatgcaa aagcaatggt caaagttttc aatcaagcaa ttggaaccag agtcatttca
    26161 ggggcgacag ataatcatct gatgttgatc gatgttcgcg agttaggtat aaatggaaaa
    26221 gaagcagaga gcattttgga cagtgtgaat atcactgtaa ataaaaactc gattcctttt
    26281 gaaacactaa gtccattcaa aacaagcggt attcgaatcg gcacgccagc aatcactact
    26341 cgcgggttca aagaagaaga tgctgtaaaa gtggctgaac tagttgtcaa agcattacaa
    26401 gcaaaagatg ataacgctca actagacgaa gtcaaaacag gtgtacgcga attaactgaa
    26461 aaatttccgt tgcataaaaa ataaaaattt aaattccatt agtaaacaga aaagttcaat
    26521 gttgggcttt tctgtttatt ttttgggaaa acggattctt tgacaatatc ggtgatgctc
    26581 tctagtatat gaggtgtaat tttagtcttc acactaaatc tcagggagga aaagtcatga
    26641 ctgatcgtat ctcgttagaa aaggaagcag tagcattcag tgatgcaaat gcgcctcatc
    26701 cgcgtatcta tgaactagca ccggaagaag gaagagtttt actagaagaa gtacaagaat
    26761 cacctgtgaa caaatattcc gtcgatattg aagacttgat ggtggatact ggcgaatggg
    26821 gaaaactcaa tattcgtctc gtacgaccag aagggaatat ggataaatta ccagttattt
    26881 tttatattca tggagcaggc tgggtttttg gcagtgctca aacccatgat aaattgatca
    26941 gagaacttgc ggttcgtacg aattccgtcg ttgtctttcc agaatatagt cgatcaccag
    27001 aagcaaaata tccaacagca attgaacaaa attatgctgt gttgcaacag ctgaataaat
    27061 tagccaagtc aaaaaatctg gatctcgaga atctaatcgt tgcaggagat tcagtaggtg
    27121 gtaacatggc tacagtcatg acgatcttaa caaaagaacg aaagggcctc ccaatatcca
    27181 agcaattgct gtattatcca gtcacaaacg ctgattttga tacagattcc tatcatgaat
    27241 tcggagaaaa ctattttttg acaagagaag gaatgatgtg gttttgggac caatatacga
    27301 cgaatgaaga agagcgtgct gaaatcacag cttcgccttt gagagcaaat ataaatgaac
    27361 taaatgatct tccttcagca atgattttaa cgggagaagc ggatgtatta agagatgaag
    27421 gtgaggctta tgcgcgcaaa cttcgtgatg caggggttcc tgttacacaa gtacgcttcc
    27481 aaggaatcat ccatgatttt gtcatggtaa atgctatgga tcagacaaag gcaacgcgtg
    27541 cagcaatggc attatctact tcttggatca agaaaaaaga ataagctttg cttgtccgca
    27601 ggaaaacttc ttttcaaaaa cggattttct taggaaaaat ggcgagtttg actagtcaat
    27661 cacttgattt tgttttacaa ttaaaaagaa acgacagaac aaaggagagc ttaaaaaatg
    27721 ggcaaatttc aagtgatcga tcatccattg attcaacaca aactaactat tattcgggac
    27781 aaaaactgcg gaacaaaggt ttttcgtgaa gtagtcgatg aaatcgctat gttgatggca
    27841 tatgaagtat cacgggatat gccattggaa gacatcgtga tcgaaacacc gatcactgaa
    27901 acaacacaaa aaacgttgtc tggtaaaaaa gtagcgatta ttccaatctt gcgcgcaggt
    27961 attggaatgg ttgacggtat cttggaattg attccagctg ctaaagtggg acatatcggt
    28021 ctttatcgag atgaagaaac attggaacca catgaatatt tcgtcaaact tccagaagac
    28081 attgatgcac gtcagttgtt tgtagtagat ccaatgttgg caacaggcgg atcagcaatc
    28141 atggcaatcg atgcattgaa agaacgtggc gcgtcgaaca ttaaatttgt ttgtttggta
    28201 gcagcacctg agggagtaaa agctttgcaa gaagcgcatc ctgatattga catctataca
    28261 gcttctctag atgaaaaatt gaatgaacat ggttatatcg ttcctggact aggggatgcg
    28321 ggtgaccgtt tgtttggtac aaaataagta aatacgaaaa agggtgtgac acaacttttg
    28381 tcacatcctt ttttggtcat ttttaacgtt tgatgcagaa cagctttttc acagctttcg
    28441 tttgcatata aaaaaaccat cccaaaaatg cagagatggt caaattcaaa tcttttttag
    28501 ttctttgtag ttaacgatta ctacaccctt actttgcgtc atcaagttcg cattttctgc
    28561 agtttcatca atctcaacaa cagcggaatt tttcaattgc tttgaaatcg ttccagtttg
    28621 cgacttgccg tgaattgtga acattacgtg agtattgatc ttgaattttt caccaatttc
    28681 cgatggtacg atagttgtat caaattttcc aaaagttgcc attttcataa aacctgcctt
    28741 tcacatcaat tataccacgt tttatgaaaa atatcatgaa attaaactaa ttttccatcg
    28801 gactctttac aatcagccaa atagagtata taatttcttt tggataaggc cagcttagct
    28861 cagttggtag agcaacgcac tcgtaacgcg taggtcgtag gttcgatccc tatagctggc
    28921 attaaaagag aagacagata tatcaaggat ttttaatcca ttgatacatc tgtctttttg
    28981 tttaaaggaa ataaagtatg ctttcttagc aatagagcat gtattatttc tataaaaatt
    29041 taggagaata aaacgactag caatatagct gtgaaatcaa atatcacatg aactgcccaa
    29101 cttgtaatga tcgagttgct tttaatggca gcttggttga ataacaatct tgccacccct
    29161 tgtatcaaaa ggatgtgtgc tacagtgtgt aaaatatttc cattatcata agtactgtaa
    29221 tggataaatc caaagatgat tgctgacaag aaactagcga caggaatgct gaacttacta
    29281 gaaaaaatag cgagaaaata cactgagaac aattcttctc caagtatcat gataggcaaa
    29341 ataataaata ataacgggct gaaagcctca tttattggat tgcctgttaa accccatttc
    29401 aaaatttgtt gcaaaaatac cgagactgcg gcactgatta ctacattgat aaaaaaatat
    29461 ttggctatat ttttgacagg agcgacaggt ttcgagaaca tttttttcgt atctttttta
    29521 ccaaaaacaa gtgtaaatgc tataaatcca gtgatagtta aagaaaaaat gctaataatg
    29581 gatggcgcaa atagcaaccc taacaaaccg agtacaacga caatagtgcc caatatccct
    29641 tttttaagtt ctattttttt tcctaaaatg ttattcatga taataactcc tactattcag
    29701 tttttgaaga tagagtcagt atacatgaag agttaaaata ggcaatgttt ttttatggat
    29761 atttgtattg ctaaaaattt atcttatata ttcattttga ttatactact tttatttgta
    29821 aaaattttct agcaaaacca agtgcttgca actgattaag cgtacaattt tttagatgtt
    29881 ccgagcgtac ttcaatcatt tcaaaggagt tgctacttaa atcgatattt tttaaatcag
    29941 tatcaaaaaa gctagcattt tctaaatcat tagtagaaaa cattatttta gtgagggaac
    30001 agtctacaaa atctgcgctt gataagacag agtgctcaaa ggtactttct tttatgtttg
    30061 aaccagataa atttacatag tccattttac aattctcgaa taacgtgttc ccaatataac
    30121 tttccatcaa tttagtacct gttaatttac aattgacaaa tttcacgcga tgcaacatgc
    30181 tttttgtcaa tatacagcct gaaaaatcac agttttcgaa tatacagtcg gtaatctcca
    30241 ttctttgaaa atcattggaa gcaaaaaatg catacttgaa aatagatttg taaataattg
    30301 gatggcagat tatttggttt tcgatgacct ctttttgtga aaggatatta tacagttcaa
    30361 aatcttcgtt ttcatagata tgaaaaaagg atcctttatt caagcttgct tcattaactt
    30421 taggaaaaat catatttaat tacctccatt ttcataaagt catattaaca aaagc
//
LOCUS       DACEOE010000012        30330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEOE010000012 DACEOE010000000
VERSION     DACEOE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.012"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1044)
                     /locus_tag="IW774_000507"
     CDS             complement(<1..1044)
                     /locus_tag="IW774_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1152883.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSVMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPY"
     gene            1124..1810
                     /locus_tag="IW774_000508"
     CDS             1124..1810
                     /locus_tag="IW774_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1152884.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(2011..2538)
                     /gene="msrA"
                     /locus_tag="IW774_000509"
     CDS             complement(2011..2538)
                     /gene="msrA"
                     /locus_tag="IW774_000509"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1152885.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(2622..3299)
                     /gene="pgmB"
                     /locus_tag="IW774_000510"
     CDS             complement(2622..3299)
                     /gene="pgmB"
                     /locus_tag="IW774_000510"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1152886.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(3296..4426)
                     /locus_tag="IW774_000511"
     CDS             complement(3296..4426)
                     /locus_tag="IW774_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1152887.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(4426..6609)
                     /locus_tag="IW774_000512"
     CDS             complement(4426..6609)
                     /locus_tag="IW774_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1152888.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(6606..7631)
                     /locus_tag="IW774_000513"
     CDS             complement(6606..7631)
                     /locus_tag="IW774_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1152889.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(7676..8503)
                     /locus_tag="IW774_000514"
     CDS             complement(7676..8503)
                     /locus_tag="IW774_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1152890.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(8500..9567)
                     /locus_tag="IW774_000515"
     CDS             complement(8500..9567)
                     /locus_tag="IW774_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1152891.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(9593..10429)
                     /locus_tag="IW774_000516"
     CDS             complement(9593..10429)
                     /locus_tag="IW774_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1152892.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(10426..11295)
                     /locus_tag="IW774_000517"
     CDS             complement(10426..11295)
                     /locus_tag="IW774_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1152893.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(11365..12648)
                     /locus_tag="IW774_000518"
     CDS             complement(11365..12648)
                     /locus_tag="IW774_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1152894.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(12662..14347)
                     /locus_tag="IW774_000519"
     CDS             complement(12662..14347)
                     /locus_tag="IW774_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1152895.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            14622..15647
                     /locus_tag="IW774_000520"
     CDS             14622..15647
                     /locus_tag="IW774_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1152896.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(16242..19895)
                     /gene="rpoC"
                     /locus_tag="IW774_000521"
     CDS             complement(16242..19895)
                     /gene="rpoC"
                     /locus_tag="IW774_000521"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1152897.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(19974..23591)
                     /gene="rpoB"
                     /locus_tag="IW774_000522"
     CDS             complement(19974..23591)
                     /gene="rpoB"
                     /locus_tag="IW774_000522"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1152898.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            24319..25287
                     /locus_tag="IW774_000523"
     CDS             24319..25287
                     /locus_tag="IW774_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1152899.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            25305..26210
                     /locus_tag="IW774_000524"
     CDS             25305..26210
                     /locus_tag="IW774_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1152900.1"
                     /translation="MINLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            26207..27013
                     /locus_tag="IW774_000525"
     CDS             26207..27013
                     /locus_tag="IW774_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1152901.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            27170..28138
                     /locus_tag="IW774_000526"
     CDS             27170..28138
                     /locus_tag="IW774_000526"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1152902.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(28455..28979)
                     /locus_tag="IW774_000527"
     CDS             complement(28455..28979)
                     /locus_tag="IW774_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1152903.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            29266..>30330
                     /gene="scm"
                     /locus_tag="IW774_000528"
     CDS             29266..>30330
                     /gene="scm"
                     /locus_tag="IW774_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1152904.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         8522 a         6544 c         5720 g         9544 t
ORIGIN      
        1 atagggatga aaaatcagac cattggcacc cgctggcaca aaagcgatag ccgaaagcag
       61 tgcatcatat ggattttctc ctttttcttt agcagattga acgacttcct gtaaaaatgt
      121 atgccctgcc caatcaaaga tgccagcacc attgctagtc gctcctccca ctacccaatg
      181 tttctgatcc agaacataac aaaacgtctc catttctgga tggaactgag gctgagtagt
      241 aataaaacga agtgctccgc tcgttcctac ggtcaaagcg gcgatcgtat caccaaaagc
      301 acctaaccct agattagaaa gcggcccgtc tgcccctccc aaaacaaatg atatatcatt
      361 tggcaaacct agttctgctg ctgtttttga tggtagacct gttacttgat aggtggaagg
      421 aaagacttgc ggcaattgct ctattcgaat gttcaagtag tcaagcactt cttctgccca
      481 ttggaattca tggatatcaa aatatccagt tcctgaagct gttccgtagt cgctgatcag
      541 ttcgccaaac aatctataaa agatatagtc tttgataccg acaaaataag cagttttttt
      601 gaataattca ggttggctgt tttgcagcca cttgattttg ttcaatggtg tcattggatg
      661 aatcggcgtc ccagtctgcc gataaaattc tttgccaagc acggtatatt tcagtttttc
      721 tgcttcgtcc gctgctcggt tatctgccca agtgatcatt cgagataacg gtgttttctc
      781 ttcatccaac aatatcaggc catgcatcac agaggagaag gagacaaatt gaatgattgt
      841 gtctgaaggc aactgtttaa ttacttcttt tataacggtg cagacacctt gaaagatcaa
      901 ttcctgatct tgttctgcca tcccttgtgt ttcttggatc aaagggtagc gaaaataagc
      961 ggatgctgct tcttgggctt catctgtgaa agcaactgct ttggtttgtg tggttccgat
     1021 atctaatccg attgaaacgt tcattttttc acctcaaatt ttatgtatgt acaaaaattt
     1081 agtttaagct tgcgtatctt ggttttgaaa agggggagat ggcgtgttac tttattttat
     1141 cattggttgc tgtatcggat catttttatg tttaaccgcc cagcggattc cgttaggtca
     1201 ctctattatt tatcctagat ctcattgtgt caaatgctgt cgttcgcttt cttggtatga
     1261 attgattccg attctctcga tcatcgtcca acgatttcgc tgccgctatt gcaggtgtcg
     1321 gttgcccttc tactatttgt tggcagaagc cttatgtggt ggtctttttg cctggttctt
     1381 tactttttct tccgcaagaa atttttcgac gttcatatgg atgctttcgg ctttgctatt
     1441 ttcgttgatg gatttatttt acttcatggt ccattctcac acgctattct gttcttgggc
     1501 aattttatgg attttttggt tgcaggccgg cgtcttccaa tggcaaagtg tgctgttaac
     1561 gtttatcgta ggcggtgtgt gccttcgcta tggtcaatcc tatctcggag ctggcgatac
     1621 tctgttgatg ctttcttgga gtggtggctt gtctctagaa gagttgcttc agatcttgtt
     1681 tcttgctagt ctgtttggaa tatccgtttt ttctgtttac tttatctgtc ataaaaaaaa
     1741 gttggaaaag ctgcctttca ttcctttttt gagcagtgcg ttgctgattg tccttcatct
     1801 taggtattga aaaaaggttg tgacaaacga cttgtcacaa cctctgatat atagtgttct
     1861 cttaccaaat ccttgtgact tggagctagg cgacaggttc acaacctctc cactaaatgg
     1921 tgttcaatca gctgaccctc agtattagct caaaatgctg gttttacaac ctctgatata
     1981 cggtgttcca taagtagctt tttcggcttc ttatttcttg acgccccaat gtcgacgaag
     2041 gaaacctgca cgtccagaac cttctcgata aagattgtaa tggagcttgt ttttcttgta
     2101 ataatcctga tggtaatctt ctgcaggata aaattcgctt cttggttcga tttttgtcac
     2161 gattggctta tcgaatcgcc cgctgtcttg caatgcttgt tttgaacgct ctgcaatatt
     2221 tcgttcttct tcatcattat agtagatcac tggacgataa gaatcaccgc gatcagcgaa
     2281 ctgtcctaat gcgtcagtcg gatcggtttg ctgccaatag atttccacca actgatcata
     2341 agaaataact tctggatcat attcgatctc tactgcttct gtatgccctg ttgtgcctgt
     2401 tgttacttgt tcataagttg gatttggtac atgcccgcca gtataaccgg atgtcacaga
     2461 aatgatccct ggctgctctt caaatggttt gaccatacac caaaagcatc cgcctgcgaa
     2521 aactgctttt tttgtcatat aaaatccccc ttttttctca tcataccttg atttccaaaa
     2581 ggaggataac gttttgcttg tgacagaaaa aacggaaact attatatacg tgatacggct
     2641 tctgataaat gccaaaacga acgtacttga tcaaaaacgg ttaaatcagg aatcacatat
     2701 tcagcagaag gaagatttat gttttcacct attcccaata catgcatgtt accagccaaa
     2761 gctgcttgac aaccagcttc tgaatcttca atgaccaagc atgcatttgg cggtacattc
     2821 aattgctgtg ctcctttgag aaaaacttct ggatcaggtt ttgcttttga tacttgcgtg
     2881 ccgtcaataa ggacatcaaa ataacttgtc aaattcgttt tttctaaaat gaggcgtgca
     2941 tttttgcttg ctgatcccaa tccgatcttg atgtttgttt ttttcaagta ctcaagaaca
     3001 gctattgcac caggcaaaac ggatgtttca tccatttttt gaatcgcttg aagatataga
     3061 ttgttttttt gctcagcaaa ggctgatttc tctttttctg agaatttctg ttccattttt
     3121 ccaaatgaca gcaatcgctc tagtgactcg gttctggata ttcctttcag ttgctcgttt
     3181 tctttctcag tgaatggaat ggacagctgg ttagctaaaa ccagccacgc ttcgtaatga
     3241 taacgagctg tgtctacaag aacgccatct aaatcgaata gtgctgcttt gattttcatc
     3301 gattacctcc ttgaaaatga acgacttgaa gtaaagatgc gatgttttta gctgtgcgat
     3361 gaagattatc agtggcttgc tgtaaagctt cttgtaaagg cacagctgca ggaaggatcg
     3421 gaaaaacggc cgaaatgcca acatctgctg ctttttcgct tcctttacca acacttccgc
     3481 aaatagctat cacaggaacg ccctctggta ctcttctagc aatcccaact ggcgcttttc
     3541 cttttagact ttgtccatct agacgtcctt cgcctacgat tacgaggtct gcttgctgta
     3601 cccgtttatc gaactccagt aaatccaata cgtagtcgat gccagagact attttccctg
     3661 aagcaaatgc taacagtcct gcacccattc caccaccagc actactgccc gatgcctcga
     3721 ttacttgagg ggcaaatctc atataaaaat catgcatggc ttggtcagcc actgctaatt
     3781 cattttcttt caagcctttt tgtggtccga aaacaaaaga agcgccttct tttccgcata
     3841 aaagattagt aacatcagta atgacagtga ttttggcttt aatcagctca ataggcacgt
     3901 tcgttttatc aactgtagca attttaccta gattttctcc cagtgcttct actttttgat
     3961 tatcttgatc gtaaaaagca taacctagac cttctgccat gccgatgccg ccatcgttgg
     4021 tactacttcc gccaacgccg atgatgatgt gtcgaacacc ttgttgcaac aattgtcgta
     4081 tcagttctcc tactcctcgc gtggataacc taagtggctg ttgttggttt agtgggacat
     4141 ctttcaatcc gacgatttct gccatttcaa aaatcgcagt ttgaccgtct gaagcgtact
     4201 ggatttcttt gtttttgcca aatgcattag taacgactgc tgtttgccgc ttcaacctta
     4261 aactgtctgc taatgatgaa accgttcctt ctcctccatc gcctataggt agcagatcat
     4321 attcagcctg aacaaaaact tcttgaaaac ctgattgaat ggcttgtgct gcctctaagg
     4381 ctgtcagact ttctttgaaa gaatctggcg caatcagaat acgcattatt ttcccctccg
     4441 ttccaagatc agttcttttt ccagctggta ttggtctcca ttgacaaaaa taggtaatgt
     4501 ttctttcgtc tcttttttta ttactatttg tttttttgtc aaaacaaata gcaacctgtg
     4561 atttttccaa ataaaaggaa aagaaatttt ttcccatgct gaaggaagtc ttggagaaat
     4621 agaaagagtt ccattcgtta ctgtaatacc tgaaaaacca aatataactg ctagccatag
     4681 tgctcctaat gaagcagcat gaattccgtc atctgatgaa tgcggatctg gtcctaaatc
     4741 aatcaggcac gcttcttgga ataaatgata cgcccattcc atttcttgga tacggcaagc
     4801 ctctatggcg tgaatcgctt tactcaacga tgagtcgtgg attgttttgt tttcatagta
     4861 atgcaaattc ttagaaacga ttggttgaga aaaatactca ggtaataagt ataacagcat
     4921 gacaagatct gcttgcttgc tgacttggag ttcatttact tcgctacggg cataatcaag
     4981 taaaatagac tggctgccag gacgttcttt gtaagtggtc aaatcaataa ctggcttact
     5041 gagatacgtg tcatcttgcg gaatcagctg ttctttgtta ggcactggca aatataactt
     5101 atctaaaaaa ttttgacatt ctttcaaaaa aagcggatcg ccagcttgat atttaacagc
     5161 taaggccaca ttataatgcg ccagatagtt cgtatatgca ttattgtcga tatattctgt
     5221 gtattcatct ggaccaatga ctctgtgtat ctctaaatga tcatcgactt ttacagctct
     5281 gctcagccaa aaagccgctg tttcatgaat gagcgttttt ccctttgttt ctaaaaacga
     5341 ttgatcctct gttgcttgga catactcttg aacggcataa gcaaggtctg cactgatatg
     5401 atgttctgcg gcagctgaag caacttcttg tcttgttccg gttttgatat tcaatgcagc
     5461 aaatttgggt gtctcttcct gaccagtgtg tgcgctttcc catgggaaaa gtgcaccttg
     5521 atagtgataa tctcttgctt tttgccgtgc gccttctaat cgatcataac ggtacagcag
     5581 caaattcttt gctacttcag gaaactgata taaaaagaaa ggcaggataa aaatttccgt
     5641 atcccagaaa acatgccctt tataaccttc gccagttagt cccttcgccc ctacactaac
     5701 acttggatca ttgcggggag tcataatctg taaatggtaa gcagcgaagt ccatcgctaa
     5761 ctgatcgaaa gaagaatcgc cttggatcgt tatccgatgc tgatgccaaa aatctgtcca
     5821 ataagacatc gactgctcta aaatttctgt ataactaacg cttttctggg cttgggcaat
     5881 acttttctta aacgggtctg tttgctgaag agaggtagag agcacgatga tcttctccag
     5941 cgtaaaggac gtattttttc tgatcttttc tgaaaaggaa gcggtgatcc ggcgattttt
     6001 tgcttggaat acacctgctt tattgaattt agctgcaatc ccaatatcaa ttttactttc
     6061 tgttgtctgg taggatgcac tgatcaaatc gtcaccaaat actcgcagtt ctttttcaac
     6121 caactgctgt gtaccaaaat tggtttgctg tccatcgata cccgttttca catctacttg
     6181 gcagtcttcg gtcaacggct tgatgaccac tttcagggct gcctgatgga gattattttg
     6241 atcagcaaca cgacaagtaa tgacttccac tttttttcct tgagcagttt caatgagcgc
     6301 atagcgttta agctctccca tttctaaatc tagtgcccgc tcgtatgcaa gaactttcat
     6361 cgtctcaaga gaaaaatgtt caccgtcgat ttttatttcc catctgatga catctgggag
     6421 attcactaac tcggctgatt ctgctcctaa aggtcggtta tatacaccag ctgcaaacat
     6481 tccccttact tgattccgat agtcttcctc ctggcttcct ctgataccaa aaaatccatt
     6541 tccaatggtc atctgactcg catacttatt gatatactct aaatcaaact gtttttcttc
     6601 tactttcata aggtaataac ttctcctgtt tctgccgaac gatataaatt ttccaccaga
     6661 ttctggatga catatccttg ccagccatct gcaatcatct gagtctggtc accgagcgcc
     6721 cgattcacaa aatgagccat acttagttca tgtgggtcat taactggcgt ggtcttttgt
     6781 aaggaaacca gctttccatc tacatcttcg aatagttcgc ctggatacag attagcacct
     6841 gcctgatcgc cacaaaaagc cacattcagt ttgtcttctc tgatattcaa agcaaacgaa
     6901 gtattcagtt ggatgatctt gccgttttct aattccagtg tgccaaaaat tgcatcttct
     6961 actgtgaatt tgcttggatc ccatttcccg aatttccctg aatttttttt cgtaccaatt
     7021 ttttggaagc tgtacgcccg aacgcttttt actttaggaa atccagtaat atacatcgct
     7081 gtatcaagca tgtgaatgcc ataatcgatc aaaggcccgc ctccttgaag ttctttgtcg
     7141 ataaacgttc cccagcctgg aacaccagac cgacgaagcg cattcgcttc tacgtaataa
     7201 atatccccga gaacatcaag atgattcttg atcattgttg cttcgtcaga aaaacgatgc
     7261 tggaatccat aagccaaaat ctgcccgttt ttatcggctg tctctgccat ctttcttgct
     7321 tgatcagctg tcatagctgg tggtttctcg cacatcactg aagcattagc ttcgagggca
     7381 gccaatacat gttcgaagtg aaagcgattg ggtgtacaga cactcacgat atcgggagaa
     7441 atctcattca gcatttgtgt aactgatgta tacgcatgag aaaatcctgt acgtcggcaa
     7501 aagtcttctg ctcgctggct gactggatca accacagcgg ccaattctaa gacatctgat
     7561 cttttttgat aataagcagc atgtactttt tctgctactt gtccagcgcc tatgattgct
     7621 actttcttca tttatttctc tctcctttgc ttatttactt attgttattc tttctctatt
     7681 ctttctctaa atcatttaaa tgacgcgctg tttccagaca gtttttcaag tattgcaatg
     7741 attcctgata aattttcact tcattttctc ctcgcaaacg acattcatat accatataac
     7801 cttcatactc attttgaagt agcgtttgaa aatggcgagt aaaatcaatt gcgccagatc
     7861 ctggttggta gcgatgatta tctgctaaat ggatatgacc cacatactct ttttgcgtag
     7921 tcaatgcttt tgaaatatca tcttcttcaa tattcatatg ataaaaatca gcgatgatct
     7981 gcacatgttt caattgatta gagtcaatgt aatgacgcgc atccgctaac aaattgatca
     8041 tatggtcttg gtatcggttc aaaggctcca aataaataac cgtacctgtt tcttcggcta
     8101 ctttatctaa ataacgaagt gattcgctga ctgctttata gtctccttct ttgcttcggg
     8161 gtgaaaccat cggcggtaat cgataagtga acattcccca agctgctggg ataacgattc
     8221 ctttgcctcc aacctcgctt agtgcacgca aaatctcagc aatttgacgt acaccattaa
     8281 gacgccgttc ttcgataaag tcaccgatcc acccatcgta gccgttacaa gctgtcacta
     8341 ctgggatccc tgtttctttg attgcctgtt tgactgtgtc gatattttct attaacaact
     8401 taccgtcgat ttcgtattct tcaaatccac agtctttgat aaatctaaat ttttctaaaa
     8461 tgtctcttgg gaaaaatgct tgatcttgcg taccgaattt cattgttctc ctcctcggac
     8521 ttgaacgccc attttgatac tttcttctgg gtgctggtct acataacgca tgtaagcttc
     8581 tgcgctttct gcaaatggca caactggatc aatgatttct tcacaatcca agtaaccgtt
     8641 catcaataat tcccaacatg tgtcctcgat tcgtttacgg ttccatcttg ggtaatctgg
     8701 gtttggttca ctagatgctc gagcaaagac gatttttgca ttattgaaat gtgcttcgcg
     8761 accaagatta aacccagctg gaaaaggttt agcaaaggca acataagaaa tgatcccgcc
     8821 atagccgatc cctcttaaag aagcttgtaa tgcggcagca ctgccactcg tttcaataac
     8881 tgaatctact ccacgtttat ctgtcaattc ctttagacga aaaccgacat ctgctttcat
     8941 aggatctagc gtgtaatcgg caccgtattt ttctgctaat tctctacgct gcgctatggg
     9001 atcgatccca acgatggtag tagcacctgc ttttttagct aattgaatcg caatttgccc
     9061 gatagcgcct aaaccgaaga ttgctacagc atcacctgct cgaacatgtg cttcccggat
     9121 accacctaaa gcgaattgag caggatcgta acatactgca tttttccatt gatccaaccg
     9181 atcaagttta cgtagtttat aattatcaac accgtttgta atcagcgttt cacgaatggg
     9241 tccataagtg cagactaact ctcctttttg atactctgtc accaaactgc ccgtttcttc
     9301 gatcgtaccg acaatcatat ttcctaattg aaattcgcca aataccacac cgcgttggct
     9361 tccttcttct cttggcaaga ataggcgcca ttcttctgaa aattcttcat cgataaatgg
     9421 acttaatcct ctaaagtcga caatttctgt tcctggtttt ggagtagcga aaattactcg
     9481 gatacgtact tcgttctttt cgatctcacg gtcttcgtaa tctttgaatc cggctattct
     9541 tggttctaat gcaactaatt ttttcatctt ttctttcctc tttcttttct ttttatcctt
     9601 ttacgccgcc ttcgactaaa tcacctttaa taaatcgttc agataacgca tacataatga
     9661 tgactggtaa agcggtcacc aatgaagcgg ccatcattcg tccccaaatg tagtctggtg
     9721 tactgaataa tgtgttcaaa ccgattggta aagtaaattt ttcggaggtc gacaggaaaa
     9781 tcgaagcaaa taaataatca ttccacgaaa ccataaagca ataaacgaag acagaaatga
     9841 ttccagaaat agccaatggt ataatgatcc gaataatgat ctggatccgg ctaagaccat
     9901 cgatcattgc tgcttcttca atatcagtcg gaatcgtgtc aaaataactt tttagcatga
     9961 atatcgcagt aggaagtgtt tgaacgacca tcgtgataat cagtgctgta ttggtatcat
    10021 acaaacctag accggaaatg attttaaata atggaacaac gatcaaaata ccggaaaaca
    10081 tataaacggt gtaaaagcta gcattgattt tcgagcgagc tttgaagcgt agttttgaca
    10141 aagcgtatgc cccgctgatc ccaatcacta ctgcagttac ggaggctatc aaggagatca
    10201 ctaaactatt tttgaaatac atgatgaatg gaaaaatctc agcattgaaa atatcctgat
    10261 aatgttcaaa agtccattct ttcggaaaaa tagtttgtgt cgttgcaatt gcttcttgtg
    10321 aatccttgaa tgatgtcatc agcatgatca aaaatggaaa caaattgaca gctaatagca
    10381 acaaaatcgt cgcgtaaaat aagattttcc tgctcttttt atttttcatt tcaagtccac
    10441 cttctttcgt gagagcaaga cgaccgctgc aataatgatg aacaatagaa tcgatatagc
    10501 tgctgctttt ccaagatcat tgaacgcaaa ggctgtctga tacaaataaa cgcctaagat
    10561 attgacttta ttcgtcagta gatagacatc tgtaaacata tagaacatcc agatagatcg
    10621 taaaacaatc acagttgcta gtactggttt gattgccggc agtgtcacga tcttgaactt
    10681 ctgccaagca tttgcaccgt ccatttcagc tgcttcataa agcgttttgt cgactgtttg
    10741 caaaatagcc aaaaatgaaa taaacgcata cggatagtat ctccagatag caaacaaaac
    10801 gaccataaag aagctagcaa ctggctggtc gaaccacagc ggtgcttcgt tgaaaagctg
    10861 caacctatct actaacagat cattgacgat tccataacta ttattgaaca tatatttcca
    10921 agcaaaaatc aaagaaatag atggcgtaac ataacttaat aaaatcagtg aacgcaccgt
    10981 tttcctaaat ctgaacttgc ggttaagcaa aatagctacg gctaatccta aagctgtact
    11041 gcctgctaca actaagaatg tgtaagcaac tgttaaacct aaagaacgat aaaattcact
    11101 atcacttaaa atttcaagat aattgcttaa tccgataaaa acggcgtctg agttaggatt
    11161 cagcggcata tcgaaaaaac tgatctgaat attggaaagc attggaatca gcactaaaac
    11221 agctaataaa agcatgcttg gtgctaaaag tcccatggct aattttaagt cagaatgttt
    11281 ccttggacga ttcattgaaa cctccccctg ataatctgcc aatagctatc tacttttttc
    11341 gaacaccatt ggcagaactt cttgttattt cgtatttttt tgtgcgtcag ctaaagcttg
    11401 atcgacatct tcgtttccga cagttacttg gttcactgct tttccgatca cacctgagct
    11461 tgtgatatct cccattttcg tgaaattctt ttccccgact agtccaaaga cttgtacttc
    11521 atcaaaagca ctagcaatct cagatggcag ctcgccaaaa gacttgatga cttcattttc
    11581 tttgtaacca tccagttcga ctacttgttt attcactggt tgagcaccac caggagacat
    11641 caagacccac ttttccatgt ttttcggttg agataagaaa gctgtgaatg cttcagcagc
    11701 ctctttttgt ttctcgtcta gaccagaaga aatcgtcaat ccagatactg tgccatagac
    11761 agcttgatct ttttgtgttg ggatagcaaa accgatattt tctgagttcc ccgcttcata
    11821 aacagaaggc aaaatgtaag ttgaatagat tgccattggt gctgttccat tcatgaaagc
    11881 atctttgatt tctgtcacat cattagagcc tggcatcgtg tattgggata agttttgata
    11941 aaacgacaat gcttctctca tcttttccgt atcgatcgtt acctcgcctt catcatccaa
    12001 aacatttgct ccatttgaca aagcaaattg agagaacgct tgctcactca ttgttccttc
    12061 agcagtaggc atagcaattc catattgttt attctcttta tctgtgaaat gttgagcaat
    12121 ttttaaaata tcatcccaat tttctggttc tgaaaaacca gcttctgata atttttcttt
    12181 gtcataccaa ataccttgaa cccagccgct aattggtgca gcaatatagc tttttccatc
    12241 ttctgaacga acaaggtttt tggctccgtc ataataatta tcttctccag cttcctcgat
    12301 aacgttttgg actgccgtct gatcaatcag ttcatcttta tccatcactt tagcaaaatc
    12361 ctgactgact tccattactt ctggtaattt accggaacga gctaacgtaa cgatttttgt
    12421 attgaacgct gattcttcta ccggcacttg cttgattttg atgtctggat tttctttttc
    12481 aaaatcagca accagttcat tgatcacact taatcgttcc tgttcgacag aagagtgcat
    12541 gaattgaatc gttgtctgtt gtgattcgtt agagctttga ctttcaccac aagcagtaag
    12601 aagaaatcca gtagctagca gacaggtagt aacaataatt tttttcattt ttttccacct
    12661 cttattttat tattttcagc cacatatatt catacggctg gaatatcaac tttcgttctt
    12721 gactgcaatg gataatttgt ccagaaaaca ggtctaggta atctcctttt ggtaaaagat
    12781 aacctgctga ttgattggaa aaattatgga taactaagac tttctcttcc ttcgcttcgc
    12841 gaataaaact gataaaagaa tctgctgagt ccagtaagta aatcaaacta ttcggatgga
    12901 aggctttttc tttttttcga atagatagca tctgggtcaa ttcaccatat actgctgttc
    12961 ttaatggatc tgtctgcaac tctttctcaa tatctgctaa caaaaatttt tgccgattga
    13021 tttttcgatt ctctcctgtt ttttctacgc ctttgtaatc atttcgactt cccagcacac
    13081 tttgaatata aattgccggc acgcctggta acgttaccaa aaatgaatga gcagctaaaa
    13141 agcgtttgct tctccattcc gtcgtatctg ttggagaact taacgcatct agatacgtag
    13201 cgtttatctc atatggactg gtcgttccat ctgggttttt cttgtagttt actcgggcac
    13261 cctctttctt cagctgttcc actaattcaa ggatcgtttt ttccggcaga atacctcgta
    13321 ccggattcaa tccgataccg tcatgggagg ctaaaaaatt aaaaaaagtt gtttttttcg
    13381 atgcgaaatc gatggttttt aaccattctt tcaacttact tgtattaccc gaacgaatcg
    13441 cttgaagggt caaaggtggt agcgaaaact gatagaccat ctgtgcttca ttctcgccat
    13501 tcccaaaata actgatatta tctttgtgtg gcacatttgt ctcagtgatc aataccgttc
    13561 ctggagctgc ttcatcagcg attgcacgga ataattggat gattttatgc gtttctggca
    13621 ggtgtataca agaagttcct agttctttcc ataaaaatcc cacagcgtcc aagcgaataa
    13681 atgaagcgcc tttttctaag taaaacaaaa gaatatcgat cattttcaat aaaacttctg
    13741 gatttttaaa attcaagtct acttgatctt cgctaaaagt cgtccatacg tgtttttctt
    13801 tcccgttttt gtcaatgaac gttgtaagta acgggctggt ccttggtcta gtcacgctgc
    13861 ttgtatctgt atccagtggc aaatcaataa aaaaatcttt gtacgtatca ttcaagttta
    13921 aatattcctg aaaccaagta ctttcggcag acatatgatt gcagacaaag tcgaacatta
    13981 accgtgtttc gcttctcatt tcttctatat cttcccatgt gcccaaacgg tcgttgattg
    14041 cctgataatt tacaacggag aaaccatcat cagatgtata tgggaaaaat ggcaagaaat
    14101 gtacaactct aaatgtatct gccaaatact tttgataaaa tttcttaaat gtacgcaaag
    14161 ttggttgctg ctcttcaaaa aattgatccc cataagtaat caagaaaata tcttcttcgt
    14221 cccaatgcag tttgcctgcc tggcctttct tctgctgata aacactgact ttttgttcaa
    14281 tcatagaggc ggttttctca gacaaatctg agtcgcgata aatcgcttgt aaaagttttg
    14341 tatacatcgg attccccttt cttttttggg agcgttccca acgatttgat catattatgg
    14401 aaaacgctta ctgtcaatag cattttttta aataattttg atatacacaa aaatacaaat
    14461 aagaaagctt atgtatcaat aattaatagc ttcttaaaaa aatatggtct ctcatgatca
    14521 tgcaagctat tcctaaattt tcaaaatcat agacaaaaaa gaatgtctat tgtactatgg
    14581 aattattcta aaacgctccc aaaaaggagg tgcatataaa attgaaacct actatttatg
    14641 acattgcgaa aaaagctaat gtctcaaaat caactgtctc cagagtatta aataaccagc
    14701 caaatatctc tgataaagca aaagcaagtg tacttcaagc aatcaaagaa ttgaattatg
    14761 aaccgagcaa aattgcaaga ggactgtcag gaaatggttt tgacgcgatt ctagcattat
    14821 ctcatcgtac ttctcatacg acctatggca atcctttttt ttcagatgtt attcaatcca
    14881 tttctgccgt ttctgaacaa aataacttcg acttgattct acaaacggca aaaacaccag
    14941 aagaagagct agaaaaatgc gcgaaaaaga tccaagaaaa aatgatccga ggaattatta
    15001 ttttaagctc tacgatggat gaaacacttc tttttgaatt ggatcaatac aatattccta
    15061 ttgtggtaat cggaaaagtc ggaaacgact acacgaacat ctactcagtg gacacggata
    15121 atttccagga tagttacgat ttggtggaaa cactgatcaa gaatggacac cgtcatatcg
    15181 gctgtctcca tgctccgaca gaagtttatg tatccaatga tcgtctcaat ggttaccgtc
    15241 aatgcttatt tgatcaccac ctagatatcc gtaatgaatg gattatagac ggcggctttc
    15301 gcttagaaga tagcatggca gcaatgaatg atttaatgaa gctacgagaa agaccaactg
    15361 cgattttttc taccgatgca ttaaaatcat tgagtattta caaagtggca gaacaatctg
    15421 gaaagcagat acctgatgat ctttcgatcg caggattcag tgatccctta ttgtcctccc
    15481 tattttcgcc acagttgact cggatagaca ttccgacaaa tcagttagga atcaaagcca
    15541 cagaactttt attccaacta atccaccacc aataccctga agaaaaaaga atcattcttt
    15601 caaccgagat tactctcaca gattcgatcc aacaacaaga aatgtagaac agaattataa
    15661 aagaacagaa acactgagga taagtcagtt actctgttct tttttcaatc actcatccct
    15721 cgttaccccc tcccggtatt ttagcgaatt tttcttattt tccatctgtt ttagatagct
    15781 gtggataact ataatgtttg tgttaaacta ttttttccac agggaagaaa aaactatttt
    15841 taaccacgtt gtgtataagt ttttatagtt cgttttttta aacacattta cccttatttc
    15901 aatccatgtt atttggggat acttatctct aaaacataaa tagaataaca gacatgtcta
    15961 ctggttttgt tttattcgag gaattattca ataatagcta gcaaaatatt aaaaataacc
    16021 gctctatctt cccatcaatt tattttgcat tcaaaaaatg atctatcttt acttttgttg
    16081 attgacttct cttggaagat gatcgttccg ataccttaat tggatacgaa ccgatgttgt
    16141 taagatcctt tcccactttt cttctagtta gacaaattat tttagacaaa ccaaaaaaga
    16201 ggttgtgaca ataatttgtc acaacctctt ttttaaaata tctattttgt ttgattgttc
    16261 attgcgtctt ctgcagccat ttgcgcttca atatctgaaa tactgtagac attttcgctt
    16321 gctacaccga cttcttttgg ttccatgtca cgataacgtt gcataccagt accagctggg
    16381 atgatcttac ctataataac attttctttc aaaccaagaa gaggatcttt cttaccgcgg
    16441 atcgcagcat ccgtcaagac acgagttgtt tcttggaatg atgcagcaga taagaaacta
    16501 tttgtttcta atgatgcttt tgtgatacct agtaagacag gtcgagaagt tgcaggaacg
    16561 cctcctgaga ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc taataatgta
    16621 cctggtaaga tatctgtatc acctggatcc attacacgaa ctttacgcag catttgacga
    16681 accattactt cgatatgctt gtcgccgatt tctacccctt gcatacggta aacacgttgt
    16741 acttcacgaa gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa caattgtttt
    16801 ggatcgattg atccttctgt caatggtgca ccacggtgga tcatatcgcc ttctgctact
    16861 ttcatacgtg ctgtataagg aacagtatac gtacgtgtat ctgtttttcc tttgattgtt
    16921 acttctttgg tacgtgttgc aggatcttct gagatatcga tgacatcccc tgttacttct
    16981 gtgatcactg cttgcccttt cggattacgt gcttcaaaga tttcttggac acgaggaaga
    17041 ccttgagtga tatcgtctcc ggcaacccca cctgtatgga atgtacgcat cgtcaactga
    17101 gtaccaggtt caccgattga ttgagcggcg atcgtaccaa ctgcttctcc gacttcaacg
    17161 tcagagccag ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg ttttgtatta
    17221 catgtgaaga cagaacggat tgtcactgtt tcgacacctg catcaacgat ttgttttgcg
    17281 atgtcttcgg aaatcaattg gtttcctgca atgatcaatt catttgtttc aggatgacgt
    17341 acttctttac gggtgtaacg tcccactaaa cgatcttcta atgattcgat gatttcattt
    17401 ccctcgcgga tggcttggat ttcaagacct cggtcagtac cgcaatcttc ttcacggatg
    17461 attacatctt gggccacgtc aaccagacga cgagtcaagt aacctgaatc ggcagtcttc
    17521 aaggctgtat cggtcatccc tttacgagcc ccgtgagttg agataaacat ttccaagacg
    17581 gatagtcctt cacggaagtt cgaaatgatt ggcaattcca tgattcgacc attaggagcg
    17641 gccatcaaac cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc acgagctcca
    17701 gaatcagaca tcatgaagat tggatttttc gcttccaaac cttccatcag tttttgttgg
    17761 atgctgtctt ttgcagcatt ccaaacagca atgacacgtt cataacgttc gtcatctgtg
    17821 atcagaccac gacggaattg ttttgtaata gtttcaactt gtttatgggc attttcaatg
    17881 atttcttgtt tttcattcag tacactgatg tcagcgatcc ctactgtgat accggcatgt
    17941 gtcgaatgtt tgtaccctaa gtctttcata cggtcaagca tcttagaagt ttctgttact
    18001 ttgaatcgtt tgaagacttc tgcgatgatg tttcctaagt ttttcttctt gaacggtaat
    18061 actaattctt gctctttgat atgtgcagga atatcagtac cagcttcaac aaagtatttg
    18121 tccggtgttt ggacggtcaa gttgaagtct gttggttcat tcagataagg gaattcagga
    18181 ggcatgatct cgttgaagat gatcttacca actgttgtga tcatcgtacg ttctttttgc
    18241 cattcagtaa atggtttttc acctaatgag ttagggttca caccgatacg tgaatgtaaa
    18301 tgaacatatc cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa gaccattcct
    18361 tcgccttcgc gtccttcttc ttccattgtc aagtagtagt tacctagaac catatcttga
    18421 gaaggagtaa caactggttt accatcttta gggttcaaga tgttttgagc agctaacatc
    18481 aacatacgtg cttctgcttg tgcttcttca ttcaatggaa cgtggacagc catttggtct
    18541 ccatcgaaat cggcattata agcttcacat acaagtggat gcaaacggat tgcgcgtcct
    18601 tgtactaaaa ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg tgcccggttc
    18661 agcaatactg ggtgttcctt gattacgtct tctaaaacat cccatacttc gtcttcttga
    18721 cgttcgattt tacgtttcgc atttttgatg tttgaagcta gttcgcgttg aacaagctca
    18781 cgcatcacga atggtttgaa taattcaatg gccatttctt ttggaagtcc acattggtac
    18841 atcttcaaga aaggtccaac aacgataacg gaacgacctg agtagtcgac acgtttacct
    18901 agcaagtttt gacggaaacg accttgtttc cctttcaaca tatgagaaag agatttcaat
    18961 gggcggttac ctggtccagt aactggacgt ccgcgacgac cgttatcgat caatgcatct
    19021 actgcttctt gcagcatacg tttttcattt tgaacgatga ttccaggtgc attcaagtct
    19081 aacaaacgtt tcaaacggtt attacggttg attacacgac ggtaaagatc attcaagtca
    19141 cttgttgcaa aacgtccacc ttctaattga accattgggc gtaaatctgg tggaataaca
    19201 gggataacgt ccataaccat ccagcttggt tgatttcctg aagcgcggaa tgcttctaaa
    19261 atatccaaac ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt caattcttct
    19321 ttcaattcgg ctgcttctgc atctagatca acattatcaa gtaattgttt aatcgcttct
    19381 gcacccatag cagcttggaa ttcttgtcca tattgttcac gtttttcgcg atattcgcgt
    19441 tctgtcaaca attgtttttt ctctaaattc gtatttcctg gttcgatgac tacatatgaa
    19501 gcaaaataaa tgatttcttc caaagcgcgt gggctcatgt ctaatacaag acccatacgt
    19561 gaaggaatcc ctttaaagta ccagatatgt gaaacaggag ctgctaattc aatatgtccc
    19621 atgcgttcac ggcgaacttt cgaacgagta acttccactc cgcatcgatc acaaacgatt
    19681 cctttataac ggatacgttt gtattttccg caggcacatt cccagtcttt tgtaggacca
    19741 aaaatccgct cacagaacaa cccttcgcgt tcaggtttta aggtacgata gttaatcgtc
    19801 tcaggttttt ttacttcacc gtaagaccag cttctgattt tttcgggaga agccaaacct
    19861 atttgcatac tttcgaattt atttacatcg atcaaaaggg gttccctcca tttcatttaa
    19921 tggcaaaccg caagacggct gatcttcgac cgccttgcag ctcatagacc ggattagtct
    19981 aaattatctt ctgcggtttc aaagttttgg atgacatcgt ctttttcatc ttccacttgt
    20041 tcagcagctt tcttttctaa ttctttggca gtttgctgtt cggcaaattt tgtcaaagca
    20101 tcaacagtga tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat ctctgtttct
    20161 tcgatatcca agacacgcat gtccagacct agtgattgca attctttcac caatacgcgg
    20221 aatgattccg gtacacctgg ttttggaatt ggttcaccct tgacgattgc ttcgtatgtt
    20281 ttcacacgtc cgactacgtc atctgatttg tatgtcaaga tttcttgtaa agtataagcc
    20341 gcaccgtaag cttccagtgc ccatacttcc atttcaccaa aacgttgtcc accaaattga
    20401 gctttacctc ccaatggttg ttgtgtaacc aatgagtaag gtccgattga acgtgcatgc
    20461 aatttgtcat cgaccatgtg agcaagtttg atcatgtaca tcacaccgac agacacacgt
    20521 ccatcaaatg gttcacctgt acgtccgtcg tacagaacag ttttcgcatc gctggccata
    20581 ccggcttcac gaactgtttc ccatacgtct tcatcacttg caccatcaaa tactggcgtt
    20641 gctacgtgga tacctaactg acgtgcagcc atacccaagt gtaattccaa tacttgcccg
    20701 atgttcatac gtgaaggcac ccctaaagga ttcagcatga tatcgattgg tgttccatca
    20761 ggtaggaatg gcatatcttc ttccggcata atacgggata caaccccttt atttccgtga
    20821 cgaccggcca ttttatcccc ttcatggatt ttacgttttt gaacgatata aacacgaact
    20881 aacatgttta cgcctggtga caattcatct ccagcttcac gagtaaagat tttcacatcg
    20941 tgaacgatac cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac ttcacgagct
    21001 ttttcgccaa agatcgcatg caatagacgt tcttctgcag ataattctgt tacccctttt
    21061 ggcgtgactt tccctacaag caaatcgcca tcttggactt ctgcaccaat acggatgata
    21121 cccatttcgt ctaaatcttt taatgcgtct tccccgacat tagggatttc gcgtgtgatt
    21181 tcttcaggtc ctaattttgt atcgcgggct tctgattcat attcttcaat atggatagaa
    21241 gtgtatacgt catcttttac caaacgacgg ctcatgatga tcgcatcttc atagttgtac
    21301 ccttcccaag tcatgaaacc aactaaaacg ttttggccta gagccatttc gccttgttcc
    21361 atagatggtc cgtctgccag agtatcccct ttttcgactt tctcacccaa atgaacgatt
    21421 ggacgttggt tgtagcttgt tccggagttt gaacgacgga attttgttac atcgtattta
    21481 tctaatgcac cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta ttcaacgaca
    21541 ccgtcatgtt tacataacaa agcagctcct gagtcatgcg ctgatttgta ttccatacca
    21601 gtaccaaccc atggtgcttg aggattgatc aacggcactg cttgacgctg catgttggca
    21661 cccatcaagg cacggttgga gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg
    21721 gcaactactt gtttaggtga tacgtccatg tagtctactt tatcaataga tacttctaag
    21781 ttttcacttt gagcacgagc cataacaacg tcttgagcaa atgttccatc ttcattcaat
    21841 gggctgtttg cttgggctac gatgtaatgg tcttcgatat cagctgtcaa gtagtcgatt
    21901 tggtcagtaa cacgacctgt ttcacggtct acacgacgat aaggtgtttc gataaatccg
    21961 aatttattta ctttcgcata actggacaaa ctattgatca acccgatgtt tgggccttca
    22021 ggagtttcga tcggacacat acggccatag tgggagtagt gaacgtcacg aacttcataa
    22081 ccggcgcggt cacgagtcaa tccgccaggt cctaaggctg aaagacgacg tttgtgtgtc
    22141 aattcgccaa gagggtttgt ttgatccatg aactgtgaca actgagaaga accaaagaat
    22201 tctttgatac ttgccacaac tggacggata ttgatcaatt gttgaggtgt caatgtttca
    22261 gtatcttgga tcgacattct ttcacgaacc acacgttcca tacgtgctag cccgatacgg
    22321 aattggtttt gtaaaagttc accgactgaa cggatacgac gatttcctaa gtggtcgata
    22381 tcatcgacat tgccgatacc ttccatcaag ttcaagaaat agctcattga agcaatgatg
    22441 tcggctggac gaaccgtttt tacagctgct tctggatagc cgtttccgat cacgtttact
    22501 tcacgttcag ggtctcttgg tgaaaatact ttgatgactt ggacagtcat tggatctgtg
    22561 accacaccgt cttctgatgg atagtaagtc actgaattta ggccattatc aatgaaagga
    22621 gctagtgttt ccatgacttg gtgagtcaga actgttcctt tttcaacgat gatctcgcct
    22681 gtttctggat caactaatgt ttcagctaat gtcagattca atagacgtgt tttcaaatct
    22741 aattttttgt ttactttgta acgaccaacg tttgctaagt cgtaacgttt tggatcgaag
    22801 aaacgagcat tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc tggacgcaaa
    22861 cgttcgtaaa cgtctttcaa accttcttct gttcttgagt cgcttgcgtt tttatgcaag
    22921 tctttttcga ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa gatcgtatca
    22981 tcggaaccaa atcctaacgc acgaactaat acggttaaag gaatcttacg tgtacggtcg
    23041 atacgtacat aagagatgtc ttttgcatca gtttccattt ctagccaagc tccacggtta
    23101 gggataacag tagaaccgaa tccttctttt ccgtttttgt cgacttttcc atggaaatag
    23161 acacctggtg aacggactaa ctgagaaacg attactcgct ctgccccgtt gatgatgaat
    23221 gttccttgtt ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc tttgatttcg
    23281 ccagtctcgc ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta gtttgcatca
    23341 tgcgcacgag cttctgctac tgtatacttt ggttctttca attcataatc tacaaattcc
    23401 aaagatagat tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat ttctctaagt
    23461 ccctcatcta agaaccattg gtaagagtct gtttgaattt cgatcaaatt tggtaattcc
    23521 aacacttcac tgattcttgc gaaacttcta cgttcgcgat gttttccgta ttttactacg
    23581 tgtccagcca agctcttcac ccctctaaaa tgtcattcaa aaagactacc cgatcaattg
    23641 ttacaataat atttcaaata ttatttttag gtaacaacag acattttttg cggcttttag
    23701 gcaaaaaaaa accaaaaata gaacattctc ttgaaacctt acttcgtgag cttctacttt
    23761 tgtttcctta tattaaagcc gaaacggaca atatttcgtt tctgcctttt cttcatcaga
    23821 tagtttttgc aacttcatat actacactta ttttctataa aagtcaacac ttcctggcgt
    23881 ttcttctgct ttttcgcaac attttcttaa tgttttcaat tttatgtttt caattatctg
    23941 acaattctta attgacagac ctttttatct cggttatact ttagaaaatt cattttgtgg
    24001 aggaacttct atgcgaacca acatcaacca aatcgcacca acacaactga ataactttta
    24061 ctttagctac tttagatagg tttgtattca tcatgatgga tacaaacgtt aaacgacagt
    24121 ttgtatctat gatggctaaa gaactttgta ttgcagcaaa aagccacgta gacaaaatgc
    24181 taagtggctg agcagtgaaa atacaaagta aaagtatcaa gcattctact ttttcaattt
    24241 attttccgac ttttcaatcc atttggacat tttgtctgaa tggatttttt gtttcccaaa
    24301 attaaggagg aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc tgccctgcta
    24361 ctagctttag gaggttgcgc acaaaaagac aattccgcag aggcatcaga tggacaaaaa
    24421 acaattggcg tattgcaact tgtacaacat aactcgttag atgctgccta taaaggattc
    24481 aaagaaggat tagcagaaga agggtataaa gaaggcgata atctaacaat cgattatcaa
    24541 aatgcccaaa acaatcaaga caacctgaaa agtatgagtg agaaactcgt caaagaagat
    24601 cctgatttgc tacttggaat cgccactcca gcagcacaat ctttattaaa tgaaacaacc
    24661 actatcccga tgactgtcac agcggtcaca gacttggaac aagcccattt agtcacatcc
    24721 aatgaaaaac ctggcggcaa tgtgactggc acgacagata tggtgccaat cgaaaaacaa
    24781 atcggcttac tgttaaatat cgtgccagac gccaaaacaa tcggcatcat gtataacaac
    24841 ggtgaagcaa actctaagat ccaagctgat ttggcagaaa aagaattgaa aaaagcgggc
    24901 gtcaaagtca aagtactgac agcaaattcc acaaatgatg tccaacaagt gacgaaaagt
    24961 ctagctaaag atgtagatgg aatctatatt cctactgata acacgttcgc aagtgcggca
    25021 agtgttgtcg gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg cgcagttgat
    25081 caagtcaaag acggcggtct agcaacagta ggaatcgact acgaaagctt gggcaaacaa
    25141 acagggatca tggcagcaaa aatcttagac ggcaagaatc caagtgaact tcctgtcgaa
    25201 acagcgaatg atttggaact gtatgtcaat aaagacatgg ccaaagcatt aggcatcaat
    25261 ccagacagca tcaaagaagc tgaataaaac acaggaggat tctcatgata aatttgctct
    25321 ctcttttctt atcatctgca tcgcaagggg tcttatgggc cctacttgcg attggtgtat
    25381 acgtcacttt ccgtatcttg gacattgccg acttgactgc tgaaggaagc tttcctttag
    25441 gtgcagggat cgcggcagta agtattacaa atggctatca tccattgaca gcttgtcttc
    25501 taggcttttt agggggtgcg gcagcagggc ttgtttccgg actgcttcac acaaaattga
    25561 aaattcccgc ccttcttgca gggatcatca ccatgactgg tttatattct gtcacatccc
    25621 gtgtgatggg tgcgccgaac atctctttat tgggtcagcc aacgatcttt cattgggtcg
    25681 agtcattcgg cataacaaaa gacaacgctg tactgatcgt cggtctgctt tttgctttga
    25741 tcgttgtatg tttacttgtt ttgttcttac gtacagaaac aggacttgct attcgtgcaa
    25801 ctggcgataa tctggcaatg agcgaagcaa acgggatcaa cacagataat atgaagatca
    25861 tcggttatat gatttctaat ggatgcattg ctctttctgg ctccttactt gcgcaaaaca
    25921 acggctttgc tgatttaaat tctggtattg gaacgatcgt aattggactt gcttctatta
    25981 tcatcgcaga agttttgtta cgtaaccagc cactatttct ccgtctcttg actgtcgttt
    26041 taggtgcggt catttatcgt tttattttag cgttagtctt cgaactgaat gttgaaccaa
    26101 gtgattcgaa acttgcttct gctttcgtgc tagtgatctg tctttctttc ccgcagatcc
    26161 agcaaaaatt ccatttacct caaaaaaaga aaggcggtag tgtcaaatga atgacgtctt
    26221 aacattggaa aatctccatc atacctttga aaaaggaacg atcaacgaaa atcatgtcct
    26281 gaaaggcatt aatctttcct tagatcccgg cgacttcgtg acgatcatcg gtggaaatgg
    26341 ggctggtaaa tctactttgc tgaacagcat cgccggtaca ttttctgtcg atcagggtca
    26401 aattttattg aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt cgaaatccat
    26461 cagtcgcgta ttccaagatc ctaaactagg gacagccgtt cgtttaacgg tagaagaaaa
    26521 tcttgctctt gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg tcaaaccaca
    26581 agaccgaaca tttttcaaag accagctggt tcggctaaat ttggggttgg aaaatcgttt
    26641 atcctcagaa atcggtttgc tgtctggagg acaacgtcaa gctattacgt tattgatggc
    26701 cacattacag cgtccagaac tgatcttatt agatgaacac actgcagcgc ttgaccctgc
    26761 tacttccaaa acggtgatgg aattgacaga aaagctgatc actgagcaga atctcactgc
    26821 ttttatggtc actcatgaca tggaagacgc gatccgctat gggagtcgac tgatcatgct
    26881 gcatcaagga aggatcgtcg tagatatcca gcaagaggaa aaacagcagc tcacagtgcc
    26941 ccaattatta gcgttgttca aacaaaacag cggcacggca ttaaaagacg atcaagtact
    27001 tttatcttca taatcagaaa gaggatggga caaaaaatga acgcccaccc tcttcttttt
    27061 tctctttcct tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg aaaactattt
    27121 tgttctctcc ttcttcctga tagaatggat gggaaaggag cttgatgcca tgacaacgaa
    27181 agacaaggta cttcaattat tgaaaaagac accagatttt ctttccggag aaaagttggc
    27241 acagcaactg gaagtttccc ggacaagtat ttggaaagcc atcaaagaat tggaaaaggc
    27301 aggctatcgt tttgagcacg ggcctacagg gtatcgttac ctgccctctg acgtactaga
    27361 tgcaaaagaa atccagcaag agctacggtc gtttattcct gatttgaaag tacaaacgat
    27421 gttgacttct gaatctacga tgaaagatgc caaactggca gcagctcaag gagattctgt
    27481 ttcgaagtta tttgttgctg atacacaaga acaagctcgt ggacgttttg gacggccttt
    27541 tttcgctgaa cctggacgag gaatctacat gagtctcttg ctccatccag acaaacggtt
    27601 tgatgaattg cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag ccattgaatc
    27661 tgtaaccggt cgttctgctt cgatcaaatg ggtcaatgat atctacatgg atggaaaaaa
    27721 agtctgtggg attttatctg aagcagtcag tgatatggaa agcggcagga tcagtcatat
    27781 catcattggg atcgggctta atttttctgt tcctcaaaat cattttccag ctgatctcca
    27841 agaaaaagcc atctctgtct ttcccgacgg acaagctagt gtcacaagga atcagctaat
    27901 cagcaaaatc tggcaagaat tgttcactta tctttctcac ccagaaaaag cgttggactt
    27961 ctataaagaa aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa aaggaacgac
    28021 ttatgtcggt cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc aaacaaatga
    28081 acagaagatg agtttgtctt ctggcgaaat cagtctcaca tctatccgta cgcaataatt
    28141 atcagatttc acaaaaggac acctgatctc atacagtgtc cttttttctg aattctcttc
    28201 aaaagactct ccttattcta tcagctgaca gtttctctat cctctagaaa aaggtattct
    28261 aaaaatatct ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa aaacaagaga
    28321 gtcatcagtt tacacgatca aaagagatag caggccgctt cctttttcaa tcttatagat
    28381 agaccatggg taacgataaa aacaaaaaag aacttgcgac ttattttgcc gtaagttctt
    28441 ttttagctag tgccttaatc tcttaatccc aacaaacgtc tcgcatagat cacctgacca
    28501 gagtgcatag cagcatcatc aataatagaa acgagacgca ttcctcgtgt gactggtggt
    28561 gtccagttct cgtcaattac ttcatctagc tcggaaggtt ccagccttct taagtaagaa
    28621 atagcaaaat cagcagctcc tcgcaagtat cctagcagat cttctatttg attagcttgg
    28681 atctgctttg cttgttccag actatgcttc caatcttgtt cgttatctgc tatcgtcatc
    28741 ggaaaccggc tttcccagtc ttgactatgc caaattggag aagttcctgc taaatcagca
    28801 atctggaaat ctaattctcg agcggtatgc caagccagcc aagtcattga tttgatctgc
    28861 ggggctctat cttccactgg aaaacgattg gcctcctcaa tagagacgcc ttcaaacaaa
    28921 cgttcaaacc gatcgtatgc ccgatccaca ctctcgcata gcatatctaa ataatccatt
    28981 tattttcctc ctttttattt atcctactta ctttttccta attctaggat acctacttat
    29041 atgttactac acacccttat tttttacact ttaacagtca ccatcatcaa tagtttggag
    29101 attgaaagaa aaagatagtg tcgaactatc aattgaacaa gaagcaacaa tatgaacgtt
    29161 tcattaatat tctttaaata aacaatgatt tttcaaacga acagtttaag actaggagaa
    29221 aaagagtata ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa aaaaagggca
    29281 agtttactag tcctagttgc tttgttgttc aatatctttt cgtatggtgt ggccgtttca
    29341 gcggaaacat acattcctaa agaccctggg aatatttata atgatctaac tggtaactat
    29401 ggcttgttag gaattgctag tcaattccac gtatttgcta aaaataagac aactgtgaat
    29461 gcacatacag acggaaatat agcaacacat gaacttgatg caaaaaataa ttttggtaca
    29521 gagcttcatg aaggtttatt gaaccaagaa ataaattata tacaaaaaac tgattctttc
    29581 gctaattctt caggcatacc tacaggtagc acaagaatga ataaatttgt cgttggcagt
    29641 aacgtggatg tagcacttca agatggtcat cctacagtca atggatccag gcttgatcac
    29701 ttaactccag atgattttta taaagatcgt gctgggcatg aatatattga tttcgagcag
    29761 gaatttgcta aattgaatgt agcagcaaat gatcttgcaa cgatcactcc agctaaaaca
    29821 tatactgctg cagattttcc agacatgaac aatcggacaa tcgatttaac tgatttatca
    29881 gataccgggt ttttattggt aaatatcgat gcagaagtgt taacgatgaa tacgccactt
    29941 caaatcatta atccaaatga tcaagtagtt gtatttaatg tcatcaacag ttcttctgca
    30001 ttgaacgtac agtcaccaat caaatacaat gatcgttcaa atcatgaaac agaagatttt
    30061 tcggatgcga atatctcttg gaattttgga aatgagatga ctgatttgac aatcagtgct
    30121 ccttttcaag gaacaatcct tgcgcctaat gctactatcc gcattactca aaaccaagac
    30181 ggaacgatta taggaacaaa tgtgatttta gatgcagcta ccaatcgttg ggatcctaac
    30241 gaaatattta tcagcgacaa tggtacagat acgacagaca caagcgacag tacagataca
    30301 tctaccacaa gtgatagctc agattcatca
//
LOCUS       DACEOE010000013        29218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEOE010000013 DACEOE010000000
VERSION     DACEOE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.013"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..680)
                     /locus_tag="IW774_000529"
     CDS             complement(<1..680)
                     /locus_tag="IW774_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1152905.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELR"
     gene            complement(746..2035)
                     /locus_tag="IW774_000530"
     CDS             complement(746..2035)
                     /locus_tag="IW774_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1152906.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(2122..2826)
                     /locus_tag="IW774_000531"
     CDS             complement(2122..2826)
                     /locus_tag="IW774_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ1152907.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            3026..3292
                     /locus_tag="IW774_000532"
     CDS             3026..3292
                     /locus_tag="IW774_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1152908.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(3460..3690)
                     /locus_tag="IW774_000533"
     CDS             complement(3460..3690)
                     /locus_tag="IW774_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1152909.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            3762..3917
                     /locus_tag="IW774_000534"
     CDS             3762..3917
                     /locus_tag="IW774_000534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152910.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(4226..4450)
                     /locus_tag="IW774_000535"
     CDS             complement(4226..4450)
                     /locus_tag="IW774_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1152911.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            4717..5052
                     /locus_tag="IW774_000536"
     CDS             4717..5052
                     /locus_tag="IW774_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152912.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5285..6226
                     /locus_tag="IW774_000537"
     CDS             5285..6226
                     /locus_tag="IW774_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1152913.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            6228..7118
                     /locus_tag="IW774_000538"
     CDS             6228..7118
                     /locus_tag="IW774_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1152914.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            7161..7961
                     /locus_tag="IW774_000539"
     CDS             7161..7961
                     /locus_tag="IW774_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1152915.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7976..8827
                     /locus_tag="IW774_000540"
     CDS             7976..8827
                     /locus_tag="IW774_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1152916.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            8824..9183
                     /locus_tag="IW774_000541"
     CDS             8824..9183
                     /locus_tag="IW774_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1152917.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            9198..9359
                     /locus_tag="IW774_000542"
     CDS             9198..9359
                     /locus_tag="IW774_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1152918.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            9356..9682
                     /locus_tag="IW774_000543"
     CDS             9356..9682
                     /locus_tag="IW774_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152919.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            9679..10494
                     /locus_tag="IW774_000544"
     CDS             9679..10494
                     /locus_tag="IW774_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ1152920.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            10633..10944
                     /locus_tag="IW774_000545"
     CDS             10633..10944
                     /locus_tag="IW774_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152921.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            10941..11171
                     /locus_tag="IW774_000546"
     CDS             10941..11171
                     /locus_tag="IW774_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152922.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            11178..11381
                     /locus_tag="IW774_000547"
     CDS             11178..11381
                     /locus_tag="IW774_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1152923.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            11378..11677
                     /locus_tag="IW774_000548"
     CDS             11378..11677
                     /locus_tag="IW774_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152924.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            11920..12333
                     /locus_tag="IW774_000549"
     CDS             11920..12333
                     /locus_tag="IW774_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1152925.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILVALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            12346..12732
                     /locus_tag="IW774_000550"
     CDS             12346..12732
                     /locus_tag="IW774_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152926.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            13283..13507
                     /locus_tag="IW774_000551"
     CDS             13283..13507
                     /locus_tag="IW774_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152927.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            13525..13953
                     /locus_tag="IW774_000552"
     CDS             13525..13953
                     /locus_tag="IW774_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152928.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            13931..15349
                     /gene="terL"
                     /locus_tag="IW774_000553"
     CDS             13931..15349
                     /gene="terL"
                     /locus_tag="IW774_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ1152929.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            15361..16896
                     /locus_tag="IW774_000554"
     CDS             15361..16896
                     /locus_tag="IW774_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1152930.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            16976..17854
                     /locus_tag="IW774_000555"
     CDS             16976..17854
                     /locus_tag="IW774_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1152931.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            17856..18173
                     /locus_tag="IW774_000556"
     CDS             17856..18173
                     /locus_tag="IW774_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152932.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            18222..18923
                     /locus_tag="IW774_000557"
     CDS             18222..18923
                     /locus_tag="IW774_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1152933.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            18938..19828
                     /locus_tag="IW774_000558"
     CDS             18938..19828
                     /locus_tag="IW774_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ1152934.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            19851..20066
                     /locus_tag="IW774_000559"
     CDS             19851..20066
                     /locus_tag="IW774_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152935.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            20078..20419
                     /locus_tag="IW774_000560"
     CDS             20078..20419
                     /locus_tag="IW774_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152936.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            20419..20790
                     /locus_tag="IW774_000561"
     CDS             20419..20790
                     /locus_tag="IW774_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152937.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            20783..21178
                     /locus_tag="IW774_000562"
     CDS             20783..21178
                     /locus_tag="IW774_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1152938.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            21180..21557
                     /locus_tag="IW774_000563"
     CDS             21180..21557
                     /locus_tag="IW774_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152939.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            21558..22166
                     /locus_tag="IW774_000564"
     CDS             21558..22166
                     /locus_tag="IW774_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1152940.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            22166..22507
                     /locus_tag="IW774_000565"
     CDS             22166..22507
                     /locus_tag="IW774_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152941.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            22749..25538
                     /locus_tag="IW774_000566"
     CDS             22749..25538
                     /locus_tag="IW774_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1152942.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNGGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVASSMESFLKVIAANIPRAVSFLSNLFDAVQKFGSALKFMMPFLVPA
                     TTAFGALMFQLKGIPTIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIVGAVVGAI
                     AVFGYFMATNEEVRNKVISVWNDVKDSVLGVLKNIKDWGIDTWNSAKEMASNAVESVK
                     DAWSGIKEWFSNTWQGIKNGATGLFDKTVETSMNAVDSVKNAWSNTKQWFSDIWQSIK
                     DSATEKWNEIKGSIMEVAGPLITGLKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LTPVLFITSMISGGWEETKNNMIGVWNNIKESALNIWESIKNIFVSYITNIYFAALNI
                     WTGFKLTLINIWNEVVSQAKSIWINVKYFFINLWIDIKYFAIQKWIELKFGIIQTWID
                     LKYNAITTWNNIKQFFKDTWQNIKDIAYNTWISIKNSMINTWNNIKESFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            25528..26268
                     /locus_tag="IW774_000567"
     CDS             25528..26268
                     /locus_tag="IW774_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1152943.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            26265..28976
                     /locus_tag="IW774_000568"
     CDS             26265..28976
                     /locus_tag="IW774_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1152944.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            28989..>29218
                     /locus_tag="IW774_000569"
     CDS             28989..>29218
                     /locus_tag="IW774_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1152945.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKI"
BASE COUNT        10269 a         4418 c         6038 g         8493 t
ORIGIN      
        1 tttcttaatt caccaatgta ttttttgttc gtcaacatat ttttaacact ttgataatct
       61 ctaacgattc catactgaga gcctaaataa taaacagttt ttcttaaact accactagaa
      121 agaaagtagt tgaatatatc tatcacgata ggagcatcat catttattac taatttctta
      181 ttttctatag agtagcctaa cggagttttc ccgcttacta cttcaccttg tgcaatttta
      241 tttgccataa cagctttgat gcgttctgaa tcaatttggg cttccaattc agcaaaactc
      301 attacttgtg caatgaaagt tctgccatac gctgtagttg tatcataata ttgttgagaa
      361 acagcattcc aagaaacatt gtgtttttct aaaatttctt gagtgtttaa ataatgtcgt
      421 agatttctaa accatctatc taattttgta aataaaatta gatctatttt attttctttt
      481 acatcttgta atagtttttg aaattcatca cgatttattt tttgaccaga aataccaccg
      541 tctacatatt cattaactac tttcaaatcc ttgtgttgtt cgatatattc atagagggtt
      601 tctttttgtt ctctaagact atctccatgt ttggcttgtt gttcagttga tactctcata
      661 tataatgcta ctcgtttcat gttatctcct ttcgaatgta tgttcttatc gttgtaaaag
      721 aaaagcccga aggctaatct ttttactaca catctgttaa ttctaatgat cttctgtatt
      781 gttcagcttg agctagttta gtaaaagata cagttccgtt ataattttct aaaacaactt
      841 tttcaatttc atcaagagat actttaaaga attcttttcg ttgattaact ttattgactt
      901 cgttttttct aaaatgcgaa tgtagttgat tttctaatgt tggtgcatct tctgaaaata
      961 tcattgcatg aacatcaaat tcaaaaggta cagaagcgct actaagttcc ttaattctat
     1021 ccataggttc taaccgtcta gtcattccta ttttatagac atcttcaccg aaagatccga
     1081 tattactgat gacataaaca tagccagctc tagtattaag ttctctttgc aatacatctt
     1141 ctttgacagt ttctagttct cttaatttct cttccagttc tttaatttta tcagcataga
     1201 gttctttttc aacatcagca ctagcttttt gcatgtatgc cattaactta gttatctctg
     1261 agttaaactg tcgttcttct ttatcaatgc gtttcttttc acgttctatt tcacgtctta
     1321 ccttttcttc ttcaatcatt tgttctttta ttgctttttg ctgttctttt tcatcgttaa
     1381 ctttaactat gtatgaatag ttgagttcta attcttgaag ttttaattca agtaattctt
     1441 ttttgaattc tgcttcatcg tttttaaaaa gcttgtttat tttatcaaaa gttcttataa
     1501 ttttttctct agttgaatct acgttagaaa tagtaagttt attgagaagt ccagttattt
     1561 ctgattctaa tggtataagc attttctttt taatattatt agcttctcta ttattcaaat
     1621 aagaaggaat attaattgca ccgtctaaaa taaaatcctt ttctttaatc tttaataaag
     1681 aaagtttgtt ttttatttcg gatgaagaag tttcatctat attcggaata attactgatt
     1741 tatagatagt gaccagagat gtgtcatatt tttgattaat ttcatcgtct aaccgctggc
     1801 tttcagcatt aagaatatca attttgtttt ctagcgttga caagttttta ttcaatttac
     1861 ttatgttttt ctctttttca gatataaacg tttccctagc atttaattct tgagaatatt
     1921 cctttacctt actctctaag gcagaagttt tatcttttaa ttttttagct tcagataatt
     1981 tgctactacc cataattatt aaaactattc caactaaaaa caatagtact cccattcaaa
     2041 acgcctcgct tcttttataa aataattgtc aaaatcatat aaacccttac cgaagttgac
     2101 ggctccggca tgggaatttt tttaaaatac aacttttccg ataattctaa tctgttctgg
     2161 cgatacaatt ctatcttcgt ataagtcgtt tatagaacgt aaaatgattg tttgattttc
     2221 gtaatcttta tatatctctt tacatgtaac tccgtcacca cctaattcca caatagcaat
     2281 ttccccatct tctacgtccg gttgttcttt aacgaatacg aattgtccat tcctgataac
     2341 aggttccatt gaatcgccta taataggtac tgcgaagtca gcagcacttg gaactctctc
     2401 aaattcttcc tcttcgacaa ccgtatcacc gtaagtaagt tctgttggat tggcagccga
     2461 agccgaattg tgaacagttg ggattttttc ttttttagga aaatctagga cattttcttg
     2521 tttttgttct tctaatcgat tacttgcaaa ttcataggta gcaactttat tttcgtcgtt
     2581 aagctgatta tatataggaa ggatattata tatctgatta gatatagata aatcaatatt
     2641 catcaaatca tctatatcaa cattgaatat cttagctatc tcatttaagg tttttaattt
     2701 tggtgtatat tttccttttt cccattcgct tattgaggat gaactttttc ttcctagttt
     2761 ttcggctagt tcttgttgat caatattata cttttctcta agaaacttta gattttcagc
     2821 gaacatagta acacctcctt gagttaattg taacaccgtt tcggaaaaaa tgtaacaggt
     2881 ttcttagaaa atattttagt gtttttaaag aagtatttac gcaaaataga aatcgatttc
     2941 aggaaaactg aaatatgcac ttgacttcgg aaaaaccgaa gtatataatt tagtcagaaa
     3001 gttaaagaga ggaggcgaat aaaatatgga taccgcgaag aaagaaaaat atacattgag
     3061 acaattaaga gctttaaagg gttattcaaa agaagaactg tcaaggaaaa gcaaagtgac
     3121 atcaagaaca atatttattt acgaaaacga tgtagataaa atgaggaacg gaaagtacgc
     3181 cactctagaa aaaattgcta aggcattggg tgttagagtt tccgatattt ttttagaccc
     3241 tgattcggaa aaaccgaaat caataacagg tgaaacaaaa caagtcagct aggaggtgtg
     3301 aatagtggaa gagaagaata taaaagtacc gactcttgaa gaatatacaa aaattgttgt
     3361 cgaaacagac gagcagaacc cgaaaacaat cgcagttatt acggcgaatg atattgaatc
     3421 atgcgaaggt tttcgagttc gaatgacccc tagatatgat taatgttctt tatcacgtgg
     3481 aggtatagga tcgtttccaa aactatcttt tgattgtatt tgtccattct ttttttggac
     3541 aataaactct gattgctgtt ttttagaaat ctttttacca tactcaacag catctgattt
     3601 attatcaaat agttttgtat cttcggaatt accttcacca cgtacagccc atttgccatt
     3661 tctaggagat acccattgat ttttacccat aacaattcac ctcgctttca agttaattct
     3721 attaagaggt gaaaagaaaa caaactgatg gaggtgagat tgtgagtaac aagccaaaaa
     3781 tcactttgaa tggcacacct aatcttcaaa gattagcaga tgaaatggct agaatacgtt
     3841 ctagacattg gggagttact gtggtggcaa ccataattga acaaaaagaa aagcccacac
     3901 agccggcaag caagtagtgg gcaccataga acaataatat tatatcacac actcgccact
     3961 tccgaaaaga ggagttgctg ctctcctttc aagtttgcca aagtccacgt ttcttcattt
     4021 tggcaactcc tcttcttgga ggtggtgacg gcgagagacg aaggagggat gacatgaagg
     4081 aagaatcaaa aatagggatt ttgaatgaga acaattacac aagattaatt attgaaacag
     4141 aagaagggga aaaagtagca gaagttacat tgaccgatgc aactcctgct gacggttatc
     4201 gtattcgatt aactcctaaa tatgattaac cttttggtgg gtgtggatcg tttccgtgac
     4261 tatcacggct ttgaattttt ccatctctgc catgaataaa tagctcagat ccttgatttt
     4321 tagaaatctc tctagcgatt tttactgctt ctgattttgt attagtatgt gcagtcgctt
     4381 tagaatttcc agcacctttg acattccaac caccattgga attaggtaca acatgttgat
     4441 tttttcccat agttttcacc tccatttcta tagaaatatt ttatcaaata gaaatggaga
     4501 aattcaataa acgaaaggag ggatcaaatg acattacaag agcatcaaca cttgatgcga
     4561 aaactaaatc aggaatacca cgaggaaatg aaacctagat tgctagggga acaatggcgt
     4621 gaacgccaga agaagtggtt agaaataaaa aagtgactca gccgtccaaa gcaatgagtc
     4681 acaaaccaaa aattatacct aaggagattt tatcacatga ataagaaaat tgaaaatctt
     4741 atcgaagaat tgaaacgtga atgccaaaag caaggtgttt ctattatttg tacagcccaa
     4801 aaagaagggg aacttaaaag tctcgtttat ggtgagacaa cagaaatttt actttgtctt
     4861 gctatgcaag aagaacattt agatgaaaat ctcccattgt cagcacatat tatgcgtaga
     4921 attgctgtgg atgcttacga acaagcgaaa aatgaagaag aaaatcaacc ttcaaatcat
     4981 acttttgtta ttaataacaa ggaagattta gctgatgtga tgactcgtat tctcaagggg
     5041 gaatttcaat gatgaatgaa aaaacaattg atctactcat tattttattt ttcacgttag
     5101 tgattgtagc gaaaagttat gtaatctttg gcttgttatt cgcaattacc atgcttgtgc
     5161 tttgcgtgtt agcaagcaga aaagaggtta aacatgaacg agttagcaaa tcttgacaac
     5221 tatttaactg atcctgatta tgctaaacca ccatatgaag cgccaattga tgaggaggat
     5281 gaagatgagt aaatctacct tagaaatgag ccatcaagaa tggctcgaag accgcaaaag
     5341 aggtattggt ggctcagatg ttgcaaccgt acttggatta aacaaataca aatctcctta
     5401 tcaattgtgg cttgagaaaa cgggtcaaat tgaattgaaa gatttagaaa gcgaaccagc
     5461 ttattggggt aatgttttag aagaagtggt tgctaaagag tttcaggaac gtacaggcaa
     5521 aaaagtacgc agaagaaacc aagtatttga acatccatta catccatttt taagagcaaa
     5581 tattgatcgg gaagtagtgg gagaaaatgc cattctggaa tgtaaaacag ccaatcaatt
     5641 tctcggaaaa gagtgggaag gtgaagaggt accgcttagc tatctctgtc aagttcagca
     5701 ttacatgaac gttttaaaca aagactattg ttatatcgct gttttgatcg gtggacaaaa
     5761 attcatttgg aagcggattg aacgagatca agagctgatc gatacaatca ctgaacaatt
     5821 agtagagttt tgggaaacga acgttcttgg aggtatcgag cctgtaattg acggaagtgg
     5881 agcgactgct gacttcttaa aagaaaaata tgcagatgta gaagaaaatc aaacagctct
     5941 accatcacgt tttgatgaac ttatcgagca aaaaagagaa ctcaagcgga cgaaaaaaga
     6001 aattgaatca tctattcgtc aagtagacaa cgagatcatc agtgagctag gtaaacgtga
     6061 agccagtatc ggtatcacac aaagaaacat tatcagctgg aaacttgttc gtacaagacg
     6121 gatgaacacg aagaaactag cagagaaata tccagatgtc gcaaatgatg aagagattta
     6181 taacgttact gaatcaagaa ggctaaccga aaaggagatc aaataatatg gcaacaaatg
     6241 aatcgttaaa aaatcaattg gcagaaaagc cacagaaaca agttgcacca ggacagttag
     6301 ggcttaaagc tctaatgaat acaccaacaa tgagaaagaa atttgaagaa gtacttcatg
     6361 acaatgctaa tgcttttatg tcgaatgtta tgactcttgt atctaatgac agttatcttg
     6421 cagatagtga accgatgtct atcatgagtg gtgcgttaac tgctgcaaca ttaaatcttg
     6481 ggctagataa gaatttaggt tatgcatatt tagttccatt caatagtaaa aacaagcaaa
     6541 caggaaaatg ggaaaagaaa gctcaattca tgcttggcta taaaggatat atccaattag
     6601 cccaacgatc aggtaaatac aaagcattaa atgtgatcga agtttacgaa ggagaactaa
     6661 aaagctggaa ccgactgaca gaagagtttg agtttgatcc aaatggtaga acgtctgatg
     6721 aagtcattgg atatgttggc tatttcgagt tactgaatgg attcaaaaaa actgtctatt
     6781 ggaccaaaca agaaatcgaa gctcatcgga ttgctaacaa taaagatcgc gataagacaa
     6841 agttaagtgg tgtgtgggca tctgattaca atgcaatggc acgaaaaact gttttgagaa
     6901 atcttctttc taaatgggga atcttgtcca tcgaaatgca agaagccacc acatcggatg
     6961 agagagtcca aagggttcaa gaagacggca gcattattgc tgaaacagaa gttgaagaag
     7021 atattcctga aagaaaagaa gcagaggtta tatctgaaga aaatgaagat gtacaaactg
     7081 gattatttga tgcatctaat ccgccgttaa acaaataatg agggagcttt ctcccttgct
     7141 tttctagagg agaaatacga atgaacacag gatatgtcaa attacatcga aaagtgatga
     7201 attcattcgt ttggaccaat ccttatatgt ataaattatg gagcttgtgt ttgatgaaag
     7261 cgagtcatga gaatcgcaag atacttttta atggaaaaga gatacaagtg aacagcggag
     7321 aattcgtcac agggcgcgat gctatcacat ctgagatgaa cgaaggcgtg aaacgtgaac
     7381 atcaagtgaa cagcggttct gtatggagat ggctaaaaca atttgaaaaa aacggaatgt
     7441 tgaacatcaa atcaactacg aaatacagcg ttatatcaat aaaaaactgg tctttgtacc
     7501 aaagcagtga acaacaaatg aacatcaaac gcacaacaag tgaacaacaa gtgcacacaa
     7561 acaagaatga aaagaattat aagaatgaaa agaataataa aacatcatcg tcgcaaccac
     7621 gcaaaaagcg tgtttacgac accgactcag tttactacat tctcgcggag gagttattca
     7681 aacagatttg tcagaatcag gaaatcaaaa agccgaatct gcaaagttgg gcagataaca
     7741 ttcggaaaat gatcgagatc gacaagcgga ccgaaaacca ggtacgggga atgattgaat
     7801 ggagtcagca caatgtgttt tgggcatcga atattttatc tgctaaaaaa ttgcgagaaa
     7861 aatacgacac aatggcagcg caagcaaatc gtgattataa aacaaaacaa actaaaacgc
     7921 ttgaatacga gaaatttagc acagatgagt tgcctatttg agaggaggcg taggcatgga
     7981 aactgttggg gaaatcatgg aaaagctgat acagaaagtg cttgtccaac gtggcgaatg
     8041 tcctgaatgc ggacagcctt tgtatggatg gcgtacgaag aaccctgatg gctcagaacg
     8101 ttgtaagcca acctgtatgc aatgtgggta taaagcattg cgagttcagg aagacttaca
     8161 aacagaacga atttacaacg agagcctgaa agcaagagca atcaattttt tcaaaggtgg
     8221 ttctgttgtg cctaatcaag cgttgtttga ttgcacattg cagaattatc aaattgtcga
     8281 tcaagaaaca agacaagcgg ttgaagtaac caaacgcttt gttaattcag tcttgttagg
     8341 aaatccaagt caccttgttt taactggaaa acaaggaaca ggtaaaagtc acctagcgat
     8401 ggcagcggct tgggaagtct tgaagcgatc aaactacgat aaaaaaatct tatttatcgg
     8461 gttacaggaa atgctggatc aaatcaaatt ttcgtacaac aatcctgaac tcagaaaaac
     8521 gattgaggga tcgttgattg cagatatcaa aacagcggat ttagtcatca ttgatgatat
     8581 cggttcagaa ctaggaaaag atgcatcaga tagtcgagcg tttggcataa acacgctaaa
     8641 ttcgttcttg gatgcacgac agaacctagc aacgattatc acaacgaact tgcttggtga
     8701 agaactgaaa aaagcttacg gtacgagaac aatatcaaga atgtttgtca actctgatgg
     8761 atttacgatg gtattttctc aaacagcaga caagcgcata aaaccagtga aaggtagtat
     8821 cgcatgaata aataccgtaa taaaaaaact gttcatcgag gtatcaagtt tgattctatc
     8881 gcggaagcag agtattacga tctagccttg tggcaagctg aagcgaatgg ttggaaagtg
     8941 aagctccagg aaaggtttga gctgatgccg aagtttgaac tagagggcaa gaagtatcgc
     9001 aagatcgagt atattcccga cttcacattt tacaaaagcg gcaagctggt caaagtcgta
     9061 gatgttaagg ggatgcagac aaaagacttt aagatcaagg caaagttgtt ctgtcatcaa
     9121 tatcaagtgc cgttgatatt agccaaaaaa tatcggaata cgttcaagga agagcgtttt
     9181 taacgaggtg gtccatcatg acaacagaag aagtgattca aatgcgaatt cgaagccttc
     9241 ggcgtgagat tgacgatctg gaacgaacaa aggcagtgat ggtcaatgaa acggctagaa
     9301 aggcaatcga tttgcacata gagaatttaa gaagggaaat ccgtagattg gaggaatgag
     9361 cgtggataag gaagcggctt ggcgaaaatt aatgttgctg attcaagatg agaactggca
     9421 agaagatgaa gcagtggttg ctgaagttca gcgtctagga aagattgcta acggacgtat
     9481 acgaaaaaag ccagacaaaa gaaaacagcg caaagggaaa atcgtcgttg ttttacacga
     9541 aggcaaaatt ttgatgcaag gaacagctag tgagctgtct gcagaaactg gatatacgcg
     9601 tgggactatt cgaacgtacg cttggcgaaa tcacaccgat cgaaaagggc acgaatataa
     9661 gtatttggag gaagaaaaat gaacgaaaac aaattaatca aattgggtgt agcaggagca
     9721 gtaatcgtag gtattggagt tatcggagga tttaagttct tcgaaaaaat cgataatgga
     9781 tatgtgggtg tgcgctattc aatgaacggc ggtatcaaag atgaagcact gacgcaaggt
     9841 gtgaaatttg tagggattga caaagtgatc caatatccaa ttcgcttgca aactatccaa
     9901 tcaaaaaata tttcagtatc tacaagcgac ggcaaaaaga caacgattga tatcaaatat
     9961 gactacaaag ttgattcaac taaagcagca aaaatgtaca aggaatttgg gaatatcact
    10021 tcggaagata tcgaaagtgg atggttaaaa tctaagcttc aaaaggtcgc tcgtgaagtt
    10081 tatgcgaaat atagtctgct tgatgtcctt tcaggagatt cctctaaagt tgaagctgag
    10141 gtattaacga actttgctaa atcagttgaa tctaaagggt ttgaagtcga agacgtaaca
    10201 cttggtgttc cagatgtcga taaagaaaca caaaaatcaa tcgatgcgat cattcgagct
    10261 ggtcaagaaa atgaaaaagc gaagctagac gcagaaactg caaaaactca agctgatagt
    10321 gaagcttaca agaaaacaaa agctgcagaa gcagaggcag aatctaatcg caaagtcgcc
    10381 gaatcagtaa cagacaattt gattcgttat gaagaagctc aagctcgcaa aaagcatgga
    10441 tgggtaacag taaatggagc agatactgta gttacggatg aagcaggcaa ataatatggg
    10501 attctttatg gctaaaattc tcttgttctt aggtttagta ggagcagcat atctcgtgta
    10561 tgcgctcctt tcccaaactg atgacaaaga agatgacaac aacgatgaaa tgaaatttta
    10621 ggaggagaaa taatggacga actaatcaca aaagtagagc agtgggctaa agacaaggga
    10681 ctggatcaag cagatccaaa agcacagttt ttgaaagtag ctgaggaatt cggggaaata
    10741 gcttcggcga tggcaagaag taatgatgaa ctatttaaag atagcgtagg agacgttatc
    10801 gtcacgctga ttatcctttc catgcaaaaa gggacaaacg tacaagagtg tttagaaatg
    10861 gcatacaacg aaatcaaagg acgcacaggg aaaatggtag atggtgtatt cgtgaagtcg
    10921 agtgatttgg aggatgtgaa atgaaatacg aaataccact aagtgaagcg ggcattcaag
    10981 caattatcaa tggtcgggag gttaacatag aacttcctga tggtactgaa ttagtcatca
    11041 gacaaagtta tttgaaagat atggcagctc cagtattaat tgatcgtttt aacgtgactg
    11101 attctgtggt agagaaccac ttaaaagaat ttcgatcaag tatagacgac actttcagat
    11161 tagggagttg attgacaatg aacaacagac atcgcagaat aacaaaacta agaaaacagg
    11221 aactgaatgt actaaagaca aagtttgaaa aagaatatgg aatttcagca gaagaaacat
    11281 ataaagtggc aagtcagttt gttgctgatg caagtgatgc tattcgtaag tttgggattt
    11341 cgatattaaa tgatgatcgt aaatgggagg aaatgagatg aaactaaaag acggatttta
    11401 cgctagtagt cacggcatcg gcggtttaat gctagatatg ccgacaaaga accctaaaac
    11461 acgtaagaaa ccaaaagtca aagtcggtga catggttcgc tgtgaagcag aagagttcat
    11521 ttatccgttt cgtggatatg tagaacacgt ctataatcac tcagcgatca ttcgtattga
    11581 aaacacaatg gaatgcgata agtggttagc gaaaagcaaa gagaatttag ctgtagcgag
    11641 attggtggat atggaggtta taaacaatga agtttaaaat ctttgaagag gacactcgtt
    11701 ataaattaga aaaagaatta aacgattttg cgaaaaacaa tgagattcag catatatctt
    11761 tagcaacttc taagagaggt tatgcaaatt actatgcagc tgttgtgagc tatgtaagtc
    11821 gagaagtgta actcggcaaa taaaaaaagc cggatcgctc cgactgattc aataaatcca
    11881 acacatttat tatatcacat aaaggagcgg tttgacttga tgcaattgtt acgagaggta
    11941 gatttcaaac agacaagatg taatgcgaga gatgtgctga agaactttcg gcgtttggag
    12001 cggatggcag gtcgctcttt gatagatatt aagtcgccga ttattacgga tatgccgaag
    12061 gcaccgaagc acggcaataa ggcagaagac gcgatcattc aaatgatgga tatagaagcg
    12121 gagagagacg cgattctagt ggctttgatg gctcttagtc tgattagtcg tcagatactc
    12181 tactacagct tctgtgtgcc agacagcttc tcaaactaca gaattagccg tgaagtgggt
    12241 tattcagaaa gaagtataca acggatgaaa tcggaagctc taatagagtt tgcagaagca
    12301 tataaacacg gaagaataat tgcttataaa tagggggacc atataatgtg gaaagattat
    12361 gtatcgttga aagagttgaa aaaagatctt gttttcaaaa gaattgtaga atggtcagag
    12421 agtgaattaa ttttagaaga tggaaccaaa atggaagttg tatgtagcga atcagattgc
    12481 tgcgcatggg ccgaaggtga attcaaaaat gtaaagcttg atgcagtgat tacagatata
    12541 aagatttttg ataaaggtaa ccgtctctat aatggtgacg gacatacctc ttatgctgaa
    12601 gtcgttgttt atcataatcg taatgagatc gctaaagcag aatgcacagc gaatgatggg
    12661 aatggcggct actattatag tgtttgtgct ttaaaggtca aagacaaact ttgtatagtg
    12721 actgacgcat aaaaaaatgg cggttttttg gcggaatgat ggcggttttt agccatttat
    12781 cagtgatatt atggtagtgt cgaaagatta gtgataggtc tgagacaaaa taataataaa
    12841 aggaacatcg ttttattatt gtttcacaat taagcttcga tagacagcag cggaaatatt
    12901 aagaataagg atgtgaattt taactccttc taaattgttc ttattatcta tcatccgttg
    12961 ctgtctattg ttacatacag atcgcttagg cggtcttttt attttggccg aaaacctaca
    13021 ttttcgatgg ccgattattg gaggaggaat agcatgttca aactatctga aatcattaag
    13081 aagtccgacg ttgagaaatt agagatgtta aaaaagaagt tgaagaaata gcagtctctt
    13141 cgtgaggctg ttttattttg ctcacaaaaa tagaccacta tcgggtaata gtggtcagga
    13201 attaaatgaa aaagatgtta gggttgttag ctaagtataa catcataacg cttacaaaac
    13261 aatgcaagaa ggataaatta ttatgagaac ttactggtat gtgtcattaa acaataaata
    13321 tccgaagcca atgaaaggac agcataggcg tgtagtcatg tctgttcaaa tgaaggcgaa
    13381 gtattcgatt gtagaaatga tcagagaggc aacgccagta gagattgatt attgcaagct
    13441 agtctattgt gggtgcggcc gttggaaaga ggatcatgta caaaaaaata tcagcaagta
    13501 catatagaga ggcggtgatt aaggatggaa aaaagcgata cgaaagcgaa cagatacaaa
    13561 cctactgctg cagagaaaaa actcttggaa gtattgataa atccagataa cttaggcaaa
    13621 ccggtgcaag agttgtgttc actggcgaaa ataggtagga ccaaatatta cgacgccatg
    13681 agcaaagatg gctttgttga tctagttaac gaaacaacaa tggatctgat caaaggaaaa
    13741 gcttctgatg tccttaatgc cacttacaag tatgcattga ctgaaaaagg tcatcaggat
    13801 aggaaaatga ttctgactat tgctggaatc tatgcagaca aacaagaact caaacattct
    13861 ggcggcgtcg atatccgaaa acagtatgag aaaatgtccg atgaagaatt agaagagttg
    13921 gtgaaacggt atgaaaaaat caacgactct taatcgtgtt gagtatgccg ttgttcaaag
    13981 agagctagcc attcgaaaag ctaggaaaag tttttggcta ttctgccagc ttctttatcc
    14041 agagttttac aaagatgatc ggttgtattt gaaagattta tgtcatacgc tacaagattt
    14101 ttacgaagat aaaatcgaca aaaaaatttt ggttattaac atgccgcctc gacatgggaa
    14161 gacttttaca gcgcggctat tcgttttatg gatgtttgga caaaatccaa agacgaaaat
    14221 tattaccgga tcttataacc aaatcctgtc atctttattt gcgcaacaaa cacgtgatgg
    14281 aatcatggtt gaaaacgaaa atgttaaggc tgaatacttt aaagatattt ttccagaggt
    14341 gcaaatcaaa tatggagatg ctgcaaaggg tttctggtct ttagatggtt cagaagaaaa
    14401 gaattattta gcgacttctc ctggtggaac atccactggt attggcgcga attatgtaat
    14461 tgttgatgac atcattaaaa ccgcagaaga agcctataac gagcgtgttt tagattctca
    14521 ctgggaatgg tataacaaca cgttggccca acgaatggaa cggccacgta aacagatatt
    14581 gattatgaca cgttggtcaa gtaatgattt agccggaaaa atgttgacga gacgaaaaaa
    14641 taatgttcat cagatctgtt ataaagcagt acaagaagac ggctcgatgt tgtgcaacga
    14701 gattatgacc cacgatgagt acttagatgt ggtacaagaa atgaatgtag atattgcaga
    14761 agccaactat cagcaagaac caattgacca aaaaggcagg ctatatcaga agtttcttac
    14821 ttatgatacg ttgcctgata acattatcaa aatatggaac tacaccgaca ccgccgacaa
    14881 gggtgccgat tactttgcgt ctcctgtttt tgctgagacg tctgatcatg atgcttatat
    14941 tatcgatgtc atgtatacaa aagagccgat ggaaacaact gaaatagccc acgctaatat
    15001 gattattcgc aatcgagtta ataaggtacg gattgaagga aacaatggcg ggcgtggatt
    15061 cagaagaaat tcagaaagaa ttacaaaaga gcgaggctat cgaggtgcag tatttgaaga
    15121 ctttcatcaa tcggataata aactgtctag aatactggcg aatagtgctt gggtggaaaa
    15181 tcatgtttac tatccagatg attgggcgac acgttggccg gacttttatg aggctatggt
    15241 tagttatcaa cgtgaaggta agaacgctca tgatgatgct ccggatgcag ttacaggaat
    15301 tgcagaaacg attatagaag cagatgactt cgagtttatt gcttattagg aggtgggtct
    15361 ttgttttatt ctggaatcac attgaaaaaa tacaaacgtt taagaacgaa atattcaacg
    15421 caaatcgaag aaggattatt cgatcctaac gcttttatgg aagatatgaa accctttttc
    15481 gatgatcgag aaagaaagta tcttgcttat actagcgaga aaaacgagat cgacagtcgt
    15541 gagaaaccaa aaacaaaaat cattaaggtc aataacaaga ttcatgcggg aatgtattca
    15601 gtggtcgttg atcaagcagt taaccatttc acaggaatac caattaaatg ggattatgat
    15661 gtttcggaac agaaaaggac attcattcaa cgaatgagag acaaattact gggtaatgat
    15721 atcagcgccc cttctacccc agaagaattt gaaacactca cgagacaatt aagtactatg
    15781 cggtttggaa tgttagaccc cgatactgca acatttcaag gagcgacagg gcttgctttc
    15841 agattactgg agcctgttga gacaaatgat ggttgggaac taagagcaag caacattgag
    15901 ccgtggaaag ctgaaaagta cgaaaacggc gcagtgtaca ttaaagagaa atacgatcca
    15961 tatcaaaaga agttttatga agagatgaag gttgtcacgc cgaaatcaat atttacttat
    16021 gctcgatata ctgattaccc tggcacggta caaggagcat ttaaaacgct gggtaatgtt
    16081 cctaatcctt taggcgttat caatcttacg gagtttaaaa acaatacaaa ccgttactgt
    16141 gactttgaag ttgcagagga gattagtgat gctattgatc gtgttttatc tgatcagcag
    16201 aacgagattg aacaattcaa gttagcttat atgctgatca ctggggcaga tttgtccgag
    16261 gataaagcca agaagatgat ggagcaacta gggatcatca atctgaaaga ccctaatgcg
    16321 aaagccggct acgtgacaaa ggatctacaa aaggatttca acgagtatca ttttgatctt
    16381 cttaaaaagc aattctacac ggtgtgtaaa gcaattgatt tcaatgacga agtctttaag
    16441 tcgaatagtt ctggggaagc ccgaaagtgg caaatcatta gtttagaagc taagacaaat
    16501 accaaagaac agtactttcg agaaggattg aaagagtgtg ccgaggttat ggctgcttat
    16561 ttggattttg ccgagaaagt tacaattgat ccggaaaaga tcattttcac cttctctcgt
    16621 tcgcttccaa cagatttaag ctacctagct gaggcattgc ctaagttggc accatacgtt
    16681 tctaagagga cgattcaaag tcagatcccg ttcgtcacag atgttgatta tgaaaacgaa
    16741 atgattgacg tcgaaagcag acaatcttac ccagacggcg agtatggagg tggcatcgtt
    16801 gagtcaaata gaaacgaagt actggaccaa acgtcgagaa ctggaagatt tagcaagact
    16861 caaaatggaa aatcaaacgc taaagaaact aagtagtgtc tttcctgatg cgttgaaaga
    16921 aattcaagcg gagttattat cccaagctga tttacacgat attacctatg ctgagatgat
    16981 ggaattttat agtacaagtg atcagaaaaa atatcgtgaa tacgttgaaa agaactacaa
    17041 atcactgaag atgtatgatg caaaatacaa agagttcatc gaagaatact ttccttcttt
    17101 cgactacgcc aaagtgaatc ggctgcttca aatgaggtca gatatttttc gaattcttgc
    17161 agagagtgca atagatgcag atgtgaataa atatttttcg gatcggttag aagatatctt
    17221 gcaacgttcg tatgtatcaa acgcaaacgt atttgcccag attttgaacg tagatttgcc
    17281 gaattactta tcaaaggaag aacttgaacg ttacctgaac tatccttggt caggtaagac
    17341 attctcgcgt cgtttatggg gcaatatttc atctctagaa caaaagctat ctaacgccat
    17401 tacaactagt gtagcaagtg gcgaaggagt tctcgtggca ttacgaacaa tgcgagaaga
    17461 cccagaaata tgcgatatgt tcaaaaggga ggaatcgaag tacaacaagg cgatcgaaaa
    17521 tcttgtgaga acagaatacg ctaaattcgc tcaagatggt attgagaaat cttatattga
    17581 aactggcgta gacgaataca atgtcttaac tgcaaaagat gaaaaggtat gttcaatatg
    17641 cggtggcaaa gcaaagaaca acccatacaa gttgagtgaa gcgatcattg gtgagaaccg
    17701 tgcgcctttt catggccgat gcagatgtac agatgttcct aatatgccga aattaggaaa
    17761 agatattgac gaagagtatg agcgtttatt tggcgattta ttagatgagt ttgcacatga
    17821 ttcttttgga attaatttga aacggaggaa gtagaatgaa agattttttt gaagcagtac
    17881 taacaattaa tgtaaatgct gatattgcag aagcctacaa aacagctatt gagtctgaga
    17941 accatcctaa tggcttgaga gaccattgga atggcaatta tgcctacgtg gttattggcg
    18001 atcaaactgt taattatcaa gataatactc cagttgataa gaataccgtt aatttaacga
    18061 ttcaattatt atctcattca ttaccaaatt taaaagaaac agttgattgg tatgaaaata
    18121 tgggatgtat tgttgttaga actgactaca aagaaggaaa gtctagtaat taggcttttt
    18181 tattttgtcc gaaatgacgt taaactagcg caatactggg cttggttgaa tggtggggcg
    18241 caactattaa aactcaaagc aatgcggggc gtgaaaacga atcgtggggc gaaaggagaa
    18301 tgattatgaa acacaaatca ttaatgccaa tgaatttgca atactttgcc gaaggtgacg
    18361 atcaaaagtt ttcttttgat gacttcaaat cttttgtgga atccaatgaa gaagctcaaa
    18421 aatttattca gtcacaatct caaagtgttg ccgacaaaca attggaagct tggaaacaaa
    18481 ataacttaga taaaattaaa caggatacca tcaaggaata tgaggaatct aagaaaaaca
    18541 agtcacctga gcaaattcaa ctggaaaaac tacaagctga atttgaagca gaaaaagcgt
    18601 tgcgtgtgac aagtgataat aaagcatttg ttgcagaaca aattgctgga ttagaactag
    18661 atggagagtt aaaagagtct atttctcaat ttatgctaaa taatcttgtt agttcggata
    18721 cagatttcac taagaatgct gttgaaggtt ttacaagtgt tttgaatgca attaatgaga
    18781 aacatgcaga tgcattaaaa gaactacaaa tgaagtctgc atttggtgga actcaacaat
    18841 cgaataacca ggttcagcag aacaatgaaa cattaacaaa tccagaagaa caattaggac
    18901 aaattcttca acaatttaac taggagagtg aaaaattatg aaaaaaacat ctttaaataa
    18961 tttagagtat ttggatattt caccagcgat taatgctatg caagtaccaa atacaccttt
    19021 tttaagctat ttacttggtg ctggaaaaac agagccagca aactcgacag aaattaaatg
    19081 gcgtgaatat gatatcaaca acgatgattc ttctgaaaaa cttgagggcg gagaatatcc
    19141 agatgctgaa tcaggtcgaa cttggtttaa caactatact gaaattttta gaaaatcaac
    19201 ctctgtatct ggtacattag atgctattaa tgtgaatgga gtcggaaatg aattaactaa
    19261 tcaagtagca ctacgtggta tggaaatgaa aattgatttg aaccgaaaat tgattactgg
    19321 tgtaaaagct gatgaaaatg gttctaaagg tcgtcgaatg aatgggattt tgaacttgat
    19381 caattcagca aataaggcag aaacagccac tgcgggtgca gtaacaagaa aagatatcga
    19441 tactttattt aaattgatgt atgaaaaagg ttatatggga gaaaaactat gcttgatttc
    19501 tccagatatg caggagttaa tgactgatga gttagatgga aaatcaacaa aaattgttca
    19561 gttcggggaa agagtaactt ttggattgca aattggaaat atcgtgtcta attacggtac
    19621 aggtattgct ctactagaac catcattgcc aaaaggaaca attgccgcaa tcgatactaa
    19681 ttatgtgaaa ctacgtccat tacgtgaatg gagagcagaa gaacttgcaa aaacaacgga
    19741 ttctagacgt atcggtcttg taggtgaata ctctcttgaa tacaatgctt caaactctgg
    19801 ggcaatttta aatttaaagt ctgaataaaa gggagttagt actccctttt ttgataggag
    19861 gaattacagt ggtaaaaaaa gatgaaacta aaaaagatga agtcgtgaaa tatagagtag
    19921 gtaaaactaa aaattttgtt gggtttgttc atcctaaaac tcgtagattt atcacagcag
    19981 attcaaataa cgaatttatc atttctatag atgataaaga agcaattgca attttggaag
    20041 atgcaattga tgttaatgaa atttaggaag tgatctgatg gatgaatcgc taaaaacgga
    20101 aatcattgag tctacaaaag aagattttcc agatttgagt gaagaacgca taactaattt
    20161 attagaaata attttgctag aaattgaatc atacaacact tgtaaaaatg atatttcatg
    20221 ggaaaagtta aaaagcgtga ttagtgaagt gttgtataaa ataataaaaa atgaatcaga
    20281 aaaaacagta tcttcagtta gacgtggcga tacaacgatt agttatgctt caacaacaaa
    20341 tgatgttagt gagttacttc taggctatgg tgatttgata cgaagagtta ttggttgtgg
    20401 aggattggag tttttttaat gaatgaagca gatattttag aaactactta tgaagatagt
    20461 tgtattattg aaagactgac ggacattgaa gattctaata caaatattac tattcaagat
    20521 tacaaaaaag tatatgataa tcctatttct tgcgctcttt cacaaggtca aatcgatgga
    20581 ctagcagtca tagaagatgg agagatggta aatgtttcaa ctgacacata taaattattt
    20641 gttcatccta agattaaact aaaaaaagga gatcgaataa caataactca aaaggccagt
    20701 ggcttaattt tttctctatt tgctactaag cctttttact atcctagtca ttgtgaagtg
    20761 aatttgatag gaagtgagaa aaatgggtaa tctcaaatat gaatcaaatg cagaaaagat
    20821 cattgagaat tttaaaaata tgactgtaat tgctcaaaaa gaaggaatat ccttcgttaa
    20881 cgattcaatg aataaagtcg ttagtttagc taaaccatta actcctgtaa aatcaggtaa
    20941 tttaaggcga ggatatcgtg tagtaaaagc tagaaaacta tcaagcggtc gaattgttgg
    21001 agcagttata aacaatgaac attattttaa atatgtagaa gaaggtcata ggactaaaaa
    21061 tggtggcttt gtcaaaggga aattcatgtt aactcgtgca acgaatcttg caaatatgtc
    21121 ttatattcct cgaagattta aacaaatggc aataaaaatc gttaagaaag gaaagtaaca
    21181 tgaaagatga aatcattgct gcaatcagta gcaaattaaa agaaatctat cctgatggga
    21241 caatatatct tgattcagtt atgcagtcaa ctaaagattt ttatttcgtt ttatcagtaa
    21301 tggaatctgg aactgaaaat gtaggaattg atattcaaaa tgtttctttc ttaattgata
    21361 ttgcattgat tgataataaa cctaatagaa atttaattaa tgaattagtc tcacgttgtg
    21421 ggactttttt taatacgatt acaatcgatg agcaaatatt atttccaaaa gcctatttac
    21481 ctgatgaagc agatggtgtt caacatattc gttttacatt agaatttcca caatatattg
    21541 aatggagtga aagataaatg ggagaaaaaa gaagtaaaac cggaattatt tctgtcgaaa
    21601 aacctacttg gtttcctttg gaagatgaga cagggaattt cccggtatat ggtactgcgt
    21661 tgccaatggg aacagcagta agtatcaaac caacagctaa ttatgaaaca acacaagatt
    21721 atggggattc agtggttcaa gatcaattta cagcttttgg tggtgcagaa gttgaactgg
    21781 aagcaaatgg atatactcat aaagttttat ctacaattac tggagggaaa attgttaaag
    21841 gtggtgcctt gcgttctgga gaagatattg cgcaagatgg tgcgtttgca tatagacgta
    21901 aaaaatcgaa tgggaaatac cgttatacgg tcttttataa aggacagttt gctctagatt
    21961 cagatgaaac gtcaacgatt gaaggaagta aagtaagttt tactcatcca acttggaagg
    22021 gttcatttgt agatgtacca ggacttggat atatgtattc agttgatgaa gatgatgaag
    22081 gcgtggataa agcaatgatc ggaaattggt ttacaaaagt agcaattcca attgaagaag
    22141 cagaactttc aggaggtaca gaataatgtc aaaatatcaa acaacgatta aattaacgaa
    22201 aaaagatgaa gaaggtaaat atgagcaagt acaatttaaa tctgctgaat ttttaccagg
    22261 aacagttgta gaagatgctg caggagttat ggaagaaatg caaacagcga ctgataaaca
    22321 atcagttaaa aaagctttaa gtcgtgctta ttcatttatt gcagatactt tatttgaggg
    22381 acaatttact ggtgaagatt attgcaaagg aattgatgct cgtgagattg cctcattgac
    22441 aggaaaatta ttgaagtctg ttactgcagg ttttgatgaa acttatacag agacgaaaaa
    22501 aaagtaagtg aggcgctcaa gtcaccttca tttaagtatt cgattactta ccgagaatta
    22561 gatataaaga cgcaattact cgaagcaggt tggacgttac cagaaattga acgtactgac
    22621 ttggatgagt taatgcgtct ttttgctttt agagatgcgg taaaagagca tgaagatgtt
    22681 gagtactatg ataatttcac tcaattttag gaggtgatac tttgaataac gatgacctaa
    22741 ttctgaagat gatactagat gaatctggtt ttactgccgg tatgaataat gctgtcaaaa
    22801 agttgaactc ttttgatgaa acaatcgaaa gaaaaagtag aaatggtggc agttcattag
    22861 gtagtatttg gaaaatattt gctggaagtt ttctagctag cggagtaact agaatcgtag
    22921 gagctggctt tgatctaatc aagggttcca taagtggagc ggttgatcga gtagatacga
    22981 tgaataacgc tctacgaaat ttccaaaata tgggatttag taattcagaa attatgaaga
    23041 atatcggaaa gaatgggctt ttatctcaag gtattcaagg acttcctact gctttgaatg
    23101 atgcgataag tcatgttcaa cttcttgctt cttctacagg tgatttaact cgttcaactc
    23161 aaatatttaa agctttaaat gatggaattc ttggtttcgg tggttcaact gatcaagtta
    23221 atgaagctgt tattcaatta tctcaaagtt tctcaaacgg aaaagtagac gcacaaactt
    23281 ggaactcaat gatcaacgct caacttggac ctactctttc tgctattgct aaaaagatgg
    23341 gaattacaat gggagatctg aaagaaggtt tatctcaagg taagatttct gttgaagaat
    23401 tccaaaatca attaatagaa atggatacca aaggtggcgg aggacttaaa tcattaagtc
    23461 aaatcgctaa agattctact aagggaatta aaacctctat acaaaatgca aaaacagctg
    23521 ttacacgtgg tgttggtgag gtaatagaag gattaaataa agcattagta gattcagatt
    23581 taggtggatt taaggggatt attgataaag ttgccagttc catggaatct tttttgaaag
    23641 taattgctgc aaatattcct agagcagtgt cttttttaag taatcttttt gatgcagttc
    23701 aaaaatttgg ctctgcattg aaattcatga tgccatttct ggttcctgca actactgctt
    23761 ttggcgcatt aatgtttcaa cttaaaggaa taccaacaat tataaaaagc ttcaataatt
    23821 ttaagaatgc cataatcggt gttggaaatt cactaaagat tatgggggct atagccgccg
    23881 caaatccgtt tgttttgatt gttggagccg ttgttggagc gattgctgta tttggctatt
    23941 ttatggcaac caacgaagag gttagaaaca aagttatatc cgtttggaat gatgtaaaag
    24001 attccgtact tggtgtatta aagaatataa aagattgggg aattgatact tggaattctg
    24061 ctaaagaaat ggcatcaaat gctgttgaga gtgtcaagga tgcttggtct ggaataaagg
    24121 aatggttttc aaatacttgg caaggcatta aaaatggagc aacaggttta tttgataaaa
    24181 cagtagaaac atctatgaat gcggttgata gtgtaaaaaa cgcatggtca aatacaaagc
    24241 aatggttttc tgatatttgg cagagcataa aagattcagc aactgaaaaa tggaatgaaa
    24301 ttaaaggttc tatcatggaa gttgctggtc cattgattac aggtttaaag aacgcattct
    24361 tgcatgtcac tttttattta gagaccttat ggaataatct agttgagatt ggtaaaaatg
    24421 tttttgagat tttaaaaaat gttatactta cgccagtttt gtttattacc tccatgattt
    24481 ctggtggatg ggaagaaaca aaaaacaaca tgattggtgt atggaataat attaaagaaa
    24541 gtgctctaaa tatttgggag tctataaaaa atatttttgt tagttacatt acaaatattt
    24601 attttgctgc gcttaatatt tggacagggt tcaaacttac tttgataaac atttggaatg
    24661 aagtagtaag tcaagctaaa tcaatttgga ttaatgtaaa atactttttt attaatcttt
    24721 ggattgacat taagtatttt gctattcaaa aatggattga attaaaattc gggattattc
    24781 aaacttggat tgatttaaaa tataatgcta ttactacttg gaacaatatt aaacagttct
    24841 tcaaagatac ttggcagaac attaaagata tagcatacaa tacatggatt tctataaaga
    24901 attccatgat caatacttgg aataacatca aggaatcttt ctggaatatt gttactggaa
    24961 ttgttaactc cgctgaaaat gcatggacta atctaaaaaa tggtgtttca aaagcaatta
    25021 atcgggtgaa agaaatcttt gattcattaa gggaaatcaa tttattcgaa atcggtaaga
    25081 atatcattga tggacttatc aatggtgtag tagaaaaatg gaatgcattg aaaaagacta
    25141 ttaaaggaat agctggaagt atcaaagatt ctattaaggg tgcattaggt attcattctc
    25201 catctagatg gatgagggat atggtaggta aaaatattgt tcaaggtatt atcgttggta
    25261 ttgataaaga aaaaagtaaa ttggatcaga caatgactga tttagtgaaa actccgtcgg
    25321 ttcaaccagt tattacaggg tctaatagtc aaccagtggt acaagctaaa caaaatacat
    25381 cttcaaatgc agtgaatgaa attcacttgc atttaaatgt ttatggagat ctacctgatt
    25441 cgatgattaa acaaatcgcc aagaagatga aaacagaatt gacgagacaa atgaaacgag
    25501 atgctgatgc agtaggaggg acattatatg caacttaaaa gaggacaatt ttttatcaat
    25561 caacattatt cttctgagtt taacgtatat attcaaaata ggcctgcctc tgtttcagct
    25621 agtcgtgtaa ttgaattgag agaacgtgaa ggcaatgact caattattat cgataaagcc
    25681 tattacaaaa acgttactag aaaaattgaa tgttattaca aagcaccatc gattgattta
    25741 gtgcaggaat gggaagatcg aattactgaa tggttagata tgaactctta cagtgatttt
    25801 attctttatt acgatcaaca atatatctat caagctatag taaccgaagc gccagaattt
    25861 aaaggaacaa gaaaaacagg gaacatagtt ccatttgaat ttacagttag tcttcgacca
    25921 ttcaaagaaa attatagtgg tcgttttgct attcaacaga taaaagcttt cgagttattt
    25981 aatccagaga agtatgcttc aaaaccgctt attaaattga gtggttctgg agatgcttct
    26041 ttttatatta ataatgataa atatgatttg aaactattag atagagaatt atatatagat
    26101 tctaaactag aagagtctta tcgaaagcta gatgacaatt tagaacatca agatcatgtc
    26161 actttatttt tagattttcc atttctatac cctggaaaaa atgaaatcaa atggacgaat
    26221 aacatacatt cgtttgagat aataccaagg tggtggagaa aagtatgata ccaagaatat
    26281 atagtcctac cgagacagat ttttctacga atggtttagg gattttgaag gacaccacaa
    26341 aatgtgagat atatgaagta gcaaatggaa aatatgaatt agagttggag tatccattag
    26401 gtactcgatt tgatgaatat tttgaaaatg actatcaaat aaaagcaaag tcaaatgatc
    26461 aagaagagta tcatatcttt tttattgatg ataaagatat tgataccttt ttagataccg
    26521 taactattta tgcccagagt cgtacaaatc ggcttggaag acgagcggtc acttttgctg
    26581 gagtcgactc taaaactggt cgagaagcaa tggcaattat tgaaaacaat atggataaaa
    26641 aatcagatat acgcttatat tctgatatta caactgtatc aagtacaact tttgaagcaa
    26701 gaaatgtttt aaattgtatt gctggtgaac aaggatcttt acttcagtat tggggcggag
    26761 aaattaaacg tgagcctttt aagctatctt tattaaagcg aagaggtcgt gataatattg
    26821 gcacaatccg atatggaaaa gatttatctg ggttaaaagt taagttagat tggacaggcg
    26881 tgaaaacaag aattattccg tatgctgatc ctcaaagtga tgcaggtaca actagccgaa
    26941 tttatggctc gccagtagat agcgaatata ttaataatta tcctgatgta tatacagaac
    27001 acgttcaatt cacagaagaa cagggagtta aagacgttaa tagcttaaat aaaatagcta
    27061 agaattattt caaaacaatt aatccaggct gtgataaacc gaagatttct attaccgttg
    27121 agttcgataa attaacggac accgaagaag gaaaagaatt cgctaagata agaaattatg
    27181 ggttatttga tacgtttaaa atatatcatc gaaaatataa actatatttt gaatcgaaag
    27241 ttagcggtgt tcaatatgat tcattatctg aaaaggtttt gaaattggaa gctggggatg
    27301 ctcaagttgc tttttatcaa caacaagctg ttactattca agataaatta aaagattatg
    27361 ctacgaataa ttatatgagt agttttaatg attatgtgtc ctctatgatt gcaggtcaag
    27421 gaaatgcagg cggttatgta gttttatggc caaaagaaaa accttcaaat atttttataa
    27481 tggatagccc tgatttaaac aaggcaaaag aagtattaag aatgaacaaa aatggaattg
    27541 ccttttctaa aaatggttgg aatggtcctt ttaattccgc ttggacattg gatagtatat
    27601 tcaatgctaa ttttattcaa acaggattaa taaaggcaga tatctttcaa aactctttta
    27661 ataaaactgg agatgtattg aaattagtta atggactact tcaaatttgg aataacaaaa
    27721 agaaaataat ggagttaacc aaaaaaggaa tggagttttg gaattctaat agttcaattg
    27781 gaacgattgg aacaactgat tctgctggta atccttttcc tggagcttct actcccacac
    27841 ctattgaaga taattcttta gttattcgta caaatggaga cggcaaatat attttgattt
    27901 ctcctaaagt tggtaaagga ttagttttat taggaaatgg taaagcaatt tattttggag
    27961 acttagatgt acaaggcaaa ctcacagtta acggaaaaga aatcacaggg aacaacagtg
    28021 gaggaagtga tcctggaact atccctcctc aattgacgac ggaagctgaa aaaagagcat
    28081 ggaaaatttg gacaatgctg aaagctcgtg gctattctga atatgcagct gcaggtattc
    28141 taggaaatat tcagggggag gttggagcaa gtatgaaccc tgatacagaa caacttggtg
    28201 gtccagctta tgggattgtt caatgggatg gttctgctta tccgttagtc ggatcaccaa
    28261 cttggaacgg acgagagtat gttcagcgtt tgatgaacac cgcagggatt caagaagatt
    28321 atcgaagtat tgaagcccaa gtaaaattat tagattggtg tatgttcaac ggtcaatggc
    28381 tcggaaaagt aaatccaacc acagtatcag gatttaaatc gatcaatgat gccaaaagtg
    28441 cagcgtatgc ttttgaaatg aacttcgaac gcccagcttc tgcacatcca gaacgccaaa
    28501 attatgccca atcttggtat aacaaattac atggattaac tagtccagaa cctggaggga
    28561 atttcatttg tccaattcaa aaaccagtga cagttacttc agaatgtgga tggagaacta
    28621 gtccaataaa tggcggccaa gaatttcata atggaattga tcttgtaaat ggaaatccta
    28681 atacacctgt ttttgcagca ttagatgggg aagttgttca ggctggtgct aattattatg
    28741 actggtatgg taattacgtg gttattaaac ataataatgg gaagtggaca ggttatgctc
    28801 atttgtctcg tattgatgtt tctgttggac aaaaagtcca gaaaggtgct caaataggct
    28861 tgatgggaac aactggtcca tctacaggag aacatctaca ttttcaaatt atgaaaaatt
    28921 attggccaca gccagttgtt gattttgaga atccaagaaa ttatatccaa ttttaaggtg
    28981 gtgattctat gagtaaatgg aatgtcgttt taagtacaac agaaccatat aattatgtgg
    29041 ggatgattca agttcgacaa ggcaataaga atacagaggt tatggaagcg actatagttg
    29101 aaaatggtct tccctacgat ttatcagaat gtaaggtata ttttgaatca gttgtaggtg
    29161 ggaaatatcc agtccaatta gaaacaaaaa ttgtggatgc taaaaaaggg aaaattaa
//
LOCUS       DACEOE010000014        29103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEOE010000014 DACEOE010000000
VERSION     DACEOE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.014"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1030)
                     /locus_tag="IW774_000570"
     CDS             complement(<1..1030)
                     /locus_tag="IW774_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1152946.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IW774_000571"
     CDS             complement(1112..1801)
                     /locus_tag="IW774_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1152947.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IW774_000572"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IW774_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1152948.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IW774_000573"
     CDS             complement(2328..2831)
                     /locus_tag="IW774_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1152949.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IW774_000574"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IW774_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1152950.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IW774_000575"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IW774_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1152951.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IW774_000576"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IW774_000576"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1152952.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IW774_000577"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IW774_000577"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1152953.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IW774_000578"
     CDS             complement(6443..7462)
                     /locus_tag="IW774_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1152954.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IW774_000579"
     CDS             complement(7635..8522)
                     /locus_tag="IW774_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1152955.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IW774_000580"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IW774_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1152956.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IW774_000581"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IW774_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1152957.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IW774_000582"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IW774_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1152958.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IW774_000583"
     CDS             9753..10397
                     /locus_tag="IW774_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1152959.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IW774_000584"
     CDS             complement(10453..10956)
                     /locus_tag="IW774_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1152960.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IW774_000585"
     CDS             11553..12518
                     /locus_tag="IW774_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1152961.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IW774_000586"
     CDS             complement(12617..13789)
                     /locus_tag="IW774_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1152962.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IW774_000587"
     CDS             complement(13776..14921)
                     /locus_tag="IW774_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1152963.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IW774_000588"
     CDS             complement(14935..15867)
                     /locus_tag="IW774_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1152964.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IW774_000589"
     CDS             15984..17063
                     /locus_tag="IW774_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1152965.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IW774_000590"
     CDS             17060..17689
                     /locus_tag="IW774_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1152966.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IW774_000591"
     CDS             complement(17799..18026)
                     /locus_tag="IW774_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1152967.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IW774_000592"
     CDS             complement(18129..20717)
                     /locus_tag="IW774_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1152968.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IW774_000593"
     CDS             complement(20737..21396)
                     /locus_tag="IW774_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1152969.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IW774_000594"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IW774_000594"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1152970.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IW774_000595"
     CDS             complement(22169..23035)
                     /locus_tag="IW774_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1152971.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IW774_000596"
     CDS             23144..23785
                     /locus_tag="IW774_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1152972.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IW774_000597"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IW774_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1152973.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IW774_000598"
     CDS             complement(25226..26125)
                     /locus_tag="IW774_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1152974.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IW774_000599"
     CDS             complement(26127..26924)
                     /locus_tag="IW774_000599"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1152975.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IW774_000600"
     CDS             complement(26902..27606)
                     /locus_tag="IW774_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1152976.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IW774_000601"
     CDS             27737..28312
                     /locus_tag="IW774_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1152977.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..>29103
                     /locus_tag="IW774_000602"
     CDS             28430..>29103
                     /locus_tag="IW774_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1152978.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8561 a         6169 c         5232 g         9141 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taa
//
LOCUS       DACEOE010000015        28621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEOE010000015 DACEOE010000000
VERSION     DACEOE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.015"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1097
                     /locus_tag="IW774_000603"
     CDS             <1..1097
                     /locus_tag="IW774_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1152979.1"
                     /translation="LEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLARSVKL
                     HYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANLFLIF
                     YLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAAVPFK
                     KGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAGYQMT
                     HSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLLFSLC
                     MRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYGGTSY
                     LILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1189..1944
                     /locus_tag="IW774_000604"
     CDS             1189..1944
                     /locus_tag="IW774_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1152980.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2002..4041
                     /locus_tag="IW774_000605"
     CDS             2002..4041
                     /locus_tag="IW774_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1152981.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGELLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4150..5301)
                     /locus_tag="IW774_000606"
     CDS             complement(4150..5301)
                     /locus_tag="IW774_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1152982.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5455..5985)
                     /locus_tag="IW774_000607"
     CDS             complement(5455..5985)
                     /locus_tag="IW774_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1152983.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6084..6434)
                     /locus_tag="IW774_000608"
     CDS             complement(6084..6434)
                     /locus_tag="IW774_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1152984.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(6591..8120)
                     /gene="ahpF"
                     /locus_tag="IW774_000609"
     CDS             complement(6591..8120)
                     /gene="ahpF"
                     /locus_tag="IW774_000609"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1152985.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8130..8693)
                     /gene="ahpC"
                     /locus_tag="IW774_000610"
     CDS             complement(8130..8693)
                     /gene="ahpC"
                     /locus_tag="IW774_000610"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1152986.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(8837..9499)
                     /locus_tag="IW774_000611"
     CDS             complement(8837..9499)
                     /locus_tag="IW774_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1152987.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            9683..11329
                     /locus_tag="IW774_000612"
     CDS             9683..11329
                     /locus_tag="IW774_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1152988.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11320..12402
                     /gene="uxuA"
                     /locus_tag="IW774_000613"
     CDS             11320..12402
                     /gene="uxuA"
                     /locus_tag="IW774_000613"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1152989.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(12510..14063)
                     /locus_tag="IW774_000614"
                     /pseudo
     CDS             complement(12510..14063)
                     /locus_tag="IW774_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            14211..15352
                     /locus_tag="IW774_000615"
                     /pseudo
     CDS             14211..15352
                     /locus_tag="IW774_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(15478..16557)
                     /locus_tag="IW774_000616"
     CDS             complement(15478..16557)
                     /locus_tag="IW774_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1152990.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(16660..17100)
                     /locus_tag="IW774_000617"
     CDS             complement(16660..17100)
                     /locus_tag="IW774_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1152991.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(17157..17567)
                     /locus_tag="IW774_000618"
     CDS             complement(17157..17567)
                     /locus_tag="IW774_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1152992.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(17747..18181)
                     /locus_tag="IW774_000619"
     CDS             complement(17747..18181)
                     /locus_tag="IW774_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1152993.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(18281..19129)
                     /locus_tag="IW774_000620"
     CDS             complement(18281..19129)
                     /locus_tag="IW774_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1152994.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            19518..21284
                     /locus_tag="IW774_000621"
     CDS             19518..21284
                     /locus_tag="IW774_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1152995.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            21386..22645
                     /locus_tag="IW774_000622"
     CDS             21386..22645
                     /locus_tag="IW774_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1152996.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            22792..24093
                     /locus_tag="IW774_000623"
     CDS             22792..24093
                     /locus_tag="IW774_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1152997.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            24096..24947
                     /locus_tag="IW774_000624"
     CDS             24096..24947
                     /locus_tag="IW774_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1152998.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            24960..25775
                     /locus_tag="IW774_000625"
     CDS             24960..25775
                     /locus_tag="IW774_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1152999.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            25926..27092
                     /locus_tag="IW774_000626"
     CDS             25926..27092
                     /locus_tag="IW774_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1153000.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            complement(27273..28574)
                     /locus_tag="IW774_000627"
     CDS             complement(27273..28574)
                     /locus_tag="IW774_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1153001.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKRRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         9542 a         5181 c         5374 g         8524 t
ORIGIN      
        1 ttttagaagt atacagtgcc agttcttatc gtttattgca agccgatgaa aatacaaaaa
       61 gtctattatt gcgtcaacta attttcatat ttttgagttg gagcgtgatc ttcttagctc
      121 gttcagtcaa actacactat ttacttcacc ctaaaatagc aggatacggt ttagccttat
      181 cgattttctt tttagtatta gtaagaatag ggatattcgg tgtcactgtc aacggcgcac
      241 aacgttggat ctctctgttt ggcattcaat tccagccttc tgaactggca aatctttttt
      301 tgatttttta tttaagctgg ttttttcgtg acggaaatag tagcccaaaa gatctaaaaa
      361 aaccattcct gattacagta ggtataactt ttctgatttt atttcagcca aagattgctg
      421 gagcattgat gatcctttcg attgcgtggg tcatattttg ggcagcggcg gttccattta
      481 aaaaagggat ctatctaatc gttacttttt ctgcattgct gattggagca gcaggcgggg
      541 tattatattt aggaaataaa ggttggcttc cacagatgtt taatcatgct tatgaaagga
      601 tcgcgacttt aagagattct tttatcgata gtcatggtgc gggctatcaa atgacccatt
      661 cgttttatgc actatataat ggtggcatat ttgggcgggg attaggaaat agtatcacaa
      721 aaaaagggta tcttccagag acagaaacag attttatttt ttcgattatt acagaagagc
      781 ttgggctaat cggtgcattg tgtgtgcttt tcttattgtt ctcattatgc atgagaatct
      841 tttgtttaag cagtcgctgt aaaaatcagc aagctggatt gtttctttta ggattcggaa
      901 ctttgctatt cgttcagacg ataatgaacg tgggaagtat cgcggggctg atgccaatga
      961 ctggtgtgcc attgccattt gttagctacg gaggaacaag ttatctgatc ctttctttag
     1021 gaatcggaat cacgttgaat atttcttcaa agatacaggc cgaagagttg gcgctctatc
     1081 gacctgaaaa acaataaaga gaaactgaga aactctcagt ttgaattatc ataaagtaca
     1141 atcttttatt tttcaagcga gaggttcttt tagaaagagg aattcgcaat gaaagtatct
     1201 caaaaaatca tattatctat tttgttactg cttacattag tagttggcta tttttcgatt
     1261 gagtttatcc atggattttc gtctgcaaaa cagagttcaa ctgtgaaaaa ggtagagccg
     1321 aaaagtatcc ctaccacact aaatgtggct ttgattggtt cagatgctcg ttcgaaagac
     1381 gaaaacggtc gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca
     1441 aaactgatct ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag
     1501 ataaatgcag cttactctta tgggggagtt gatttattaa atcaaacatt aaaggaaaat
     1561 ttcaaatttg aagctcccta ttatgcaagt atcacatttc aagattttat cgattgcgtt
     1621 aatgaactgt ttcctggtgg agtcaagatc gatgcagaaa aatctctaga tttagatggc
     1681 gtatatataa acaaaggaga gcaagtgatg gatggtaata ctttactcca gtatgctcga
     1741 tttcgggaag acaaagaagg agacttcggg aggatcagaa ggcagcagca agtgatcaaa
     1801 gctatctctc agcagctgaa agacgtaact tcaatattca agctacccaa agcggttggg
     1861 aaattacttg gtagtatcca aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg
     1921 gatttcctca aagacatatg ttagaataaa caggtataaa tagtgaaata aaggaatgac
     1981 aagcaagaga aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc
     2041 gcttatattg ccggcgcagt gatcttaata gcagctgcgg gtggcggtta tttttactac
     2101 cagcactatc aagaaaccca agcagtagaa gctggagaaa agacagttga gcaatttgtc
     2161 caagctttga acaagggaga ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca
     2221 aataaaagtg cattatctga aaaagagatc ttagataaat accagaatat atacggtgct
     2281 gctgatgtca aaggacttca gatatcgaat ctaaaagtag ataaaaaaga cgattctact
     2341 tacagttttt catataaagc aaagatgaat acctcattag gtgaattgaa agatctttct
     2401 tataaaggaa cattagacag aaatgatggt caaaccacga tcaactggca acccaacttg
     2461 gtttttccag aaatggaagg aaatgacaaa gtaagcctga ccacacaaga agcagcaaga
     2521 gggaatatta tagatcgaaa tggtgaacca ttagcaacaa caggcaaact aaaacaatta
     2581 ggagtcgttc caagtaaact tggagatgga ggcgaaaaaa cagccaatat caaagcgatt
     2641 gcttcctcct tcgacttaac agaagatgct atcaatcagg cgatttcaca aagctgggta
     2701 caacccgatt actttgtccc attgaaaatc attgatggag caacgccaga acttccagct
     2761 ggagctacca ttcaagaagt agacggcaga tattatcctt tgggtgaagc agctgctcag
     2821 ctgattggtt acgtgggaga tatcacagca gaagatattg ataagaatcc agaattaagc
     2881 agtaatggaa aaatcggtcg ctcaggtttg gagatggctt ttgataagga tcttcgtggg
     2941 acaacaggtg ggaaattaag cattacagat gcagacggtg tagagaaaaa agttctgatc
     3001 gaacatgaag ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa
     3061 acagcttttg acagtctagg aggaaaagct ggatcaactg ttgcgacaac gccaaaaacc
     3121 ggtgagcttc ttgcgcttgc tagctctcca agctatgatc caaacaaaat gacaaacggg
     3181 atctcacaag aagattacaa agcttatgaa gaaaatcctg aacatccatt catcagccga
     3241 tttgcgacag gttatgctcc tggatctacg tttaaaatga ttacagcagc aatcggtctc
     3301 gacaacggca ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa
     3361 gacagttctt ggggatctta tcaagcaacg cgtgtcagta gtgatgtatc acaagtagac
     3421 ttaaaaactg ctttgatcta ttccgataat atatatgcgg cacaagaaac gttgaaaatg
     3481 ggtgagaaaa aatttcgtac aggcttagat aaattcattt ttggtgaaga ccttgatttg
     3541 ccaattagta tgaatccagc acaaatttct aatgaagata gctttaactc agatatcttg
     3601 ctagctgata ctggatatgg acagggcgaa cttctaatta ctcctatcca gcaagcagca
     3661 atgtattctg tttttaccaa caatggcaca cttgtctatc ctaaattgat tgcagataaa
     3721 gagacaaaag ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat
     3781 ctgagagaag ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt
     3841 ccgttggcag cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa
     3901 gagaacagtt tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg
     3961 ttggaaaata aagaagatga tgattcagca accaaacgag catcggaact attgcaatac
     4021 ctcaaccaaa attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt
     4081 atcgtcacag tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt
     4141 ttgatttact cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat
     4201 aagtgtactt aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg
     4261 actttgctgt ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc
     4321 accgaccatc aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc
     4381 aagcaatttg gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact
     4441 aaagtcagaa aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc
     4501 atatcctaaa gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc
     4561 tatcccagca aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga
     4621 cattcctaaa caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc
     4681 tgcaaatgaa agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata
     4741 aattagctgt tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag
     4801 aaaagatagg ctgaaactta gaactaaaac gactaaaatg tcatctacca cagaagcacc
     4861 caaaattgtt gatccttctt ttgtattcac tacgtttagt tcttttaata cttctactga
     4921 aatactgaca gaagttgccg caagaataat cccaaagaaa atcgcttcat ttcccgaaac
     4981 ttggaatgct ttgcctgtca accagccaaa aacaaccggg aagatgatcc caagcaaagc
     5041 aacgaacata ccaggtcgga aatatttttt caaaagagat aaatcacttt ccagaccagc
     5101 taaaaacatt aataagatca caccaatctc cgaaaagtca tggactagaa tatcaggttt
     5161 gacccaattc aatccagctt ggcctagcaa aacaccaaca agtaattgtc caatcactgc
     5221 tgggatacct aatctccgcg agatatgtga gccaatcgtt gttgaaatca atatcaggca
     5281 taaaatacca ataaattcca ttctcatcat tcctttgtat aaaatatagc ccgaacttag
     5341 cataacatac attattgaaa aaataggaaa atatactcac aaaaaaacca acaatccaaa
     5401 agattttttt ggattgttgg tttttatacg gtctatttta ataactaaaa atgattaacg
     5461 tatagtcatt tttcttattc tgatgaaatt gataatctcc tggatgccca tgaaaatcaa
     5521 aataaagccg aatatttgga atagaagcat caaacttttg aatggattga acaataagac
     5581 gaatccgcca atcaacaaca gaatactact ataaacactc caacttttag cttttgtatt
     5641 catgctgaag aacaactgga acaagctgtt gatgataaca gctaatccaa gaagaatcgg
     5701 caggatagaa acgattgcag gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa
     5761 aagaccactg aacagaccga tactccaaga acctgttttc cttttaaaac ggtaagcctc
     5821 aaaaagattc aacaacccaa gcaaaacaag ataggcggtg atcagataac cgataaaatg
     5881 aaaaacagca gaagggttta gcacaatcgc aatcccagaa ataatataaa cagcagctct
     5941 taataaggcg tatttttgaa tattttgttt aatcgtctca aacatataag aaaacctcct
     6001 ttgacttatt ttaacgtgaa ctagtcattt taccaaatta aatgttacaa aatgagcgaa
     6061 ggagaagaat atctctggaa aaatcaactt ctgaggatct tttccatttt tttgcctttt
     6121 gctagctcat cgatcatttt atctaaataa cgaatttctt tcatcaagtt atcctcgatg
     6181 tcttctactc gtacaccaca aacaacacct gtaatcaagg aacgtgaagg attggggaca
     6241 ggggcttccg caaaaaattg ttcgaaagtc acttgttttt gtagttgata ttctagttct
     6301 gtttgggaat aacccgtcag ccaacaaatg atttcatcta cttcctcttt tatacgatct
     6361 tttctctccg cttttgctac atacatagga taaacgcttg caaaacttgt cgtaaaaata
     6421 cgattttgcg ccataatcag tcacctttct ttttttcaaa ttatttacta cttatagtat
     6481 ataagaaaaa gagaagaaaa agcgattatt caaaaaaaca aaaaggattg tgacaaaatt
     6541 cttgtcacca tccttttttc cgctctctga ctttttacaa tcttcatagc tcaagtgcgc
     6601 atcagataat cgaatgctcc taaagctgca gtagctcctg aacccattga aacaatgatt
     6661 tgtttgtatg gactatctgt acaatcacca gctgcgaaaa cgccggggat attcgttgcg
     6721 ccatttttct cggtcactat ttcatttctt ggattcattt cgatcgttcc atctagccaa
     6781 tccgtattag ggagtaagcc gactagaatg aatactcctg aaacagctag tgtatgcatc
     6841 tccatcgtac ggcgatcgat ataggagaga gattctacct gattcgtacc gctgatttct
     6901 ttcgtcgctg catgtttgat aacagtgaca ttcgcaagag agactagttt atcctgtaag
     6961 atttgatctg cttttaattc tggtaagaat tctagaacat atacatgttt tgctaaccca
     7021 gccaaatcga ttgctgcttc aacaccagaa ttaccaccac cgataacagc aatctctttt
     7081 cctttaaata aagggccgtc acagtgtggg caataagcaa ttcctttatt tttaaactct
     7141 ttttcgcctg gtacattgat agaacgccag tgcgcacctg tcgcgatgat ggcggttttt
     7201 gctttaagtg atataccgtt agctaagcca atattaataa aatgttgctc tttatttaag
     7261 gaaacggcct gttgattcat cataatgtcg atagggtaac tacgcacatg actttctact
     7321 tgctgcatca attttggacc ttctgtgtaa gaggtaccaa tcatattttc aatccctagt
     7381 gtttcaacga cttgtccgcc gaaagaatca gcgacaagtc ctgtacggat tcctttacgt
     7441 gctgcataga tagctgcact actagctgct ggtccaccgc caatcactaa tacatcataa
     7501 ggttcttttt caaacaactc ttcttcaatc aaagggccag tgattttttc aacaatttgt
     7561 tcgatcgtca tgcgtccgct actaaattct tctccattta aaaacacagt tggcactgcc
     7621 atgatttttt tatcatcgat ttctttttgg aacatcccac cttcaatcat cgtatgagta
     7681 atatttggat ttaatacagc taatatattc aatgcctgta caacgtcggg gcaattatga
     7741 caagtcaagc taacataact gtcaaaatgc aatgtttgtg ttattttttt gattttaaga
     7801 ctcaaagatt cactgatttt cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa
     7861 gaggaaaatt catgaccgag ggggatacca gcaaaaacga ttccactttc tttgccaact
     7921 ggattgatag aaaaactagg tgtgcgcatc gaatctcttt tttcccaaga gattttagga
     7981 gacatttctg ctatttcctt cgtaaattca gctagttttt gactattttc actattatcc
     8041 aaactaacag aaaaaacaat aggagattca atcagtgcta aatattgttc gagattttgt
     8101 ttagtttctt tgtctaacat atccaccacc taaatctttc caacaagatc aaaacttggt
     8161 ttcaatgttt cgccactttc tttccatttt gccggacaaa cttcacctgg gtgggtacgt
     8221 acatattgag cagcgcgaat cttatcaatc agtgcactag cgtcacgacc gattccatct
     8281 gcattgattt ccatagcttg aacgattccg tctggatcaa taataaatgt tcctcgctga
     8341 gctaatcctt gctcttcatc caaaacatca aaaatacgag aaatctgatg agacggatca
     8401 ccgatcatca cgtattcgat tttaccgata gcatctgatg tatcatgcca tgctttgtga
     8461 gtaaagtggg tatcggtaga gcaagaaaag acttcaacac ctaaagattt caatgtactg
     8521 tattgttctt gtaagtcttc taattcagta ggacaaacaa aagtaaaatc agctggatag
     8581 aagcaaagaa tactccagtt ccccataaga tctttttcac tgacttctaa aaattctcct
     8641 tgatgataag catgtgcatt aaattcttcg atttttttcc caatcaatga catagtaaaa
     8701 cctccaataa taattattta tttataatta ttgtaattta gttggagttg caaagtcaat
     8761 ggatattttg ttatttaata aagattactc aaaaacgaag gttaattata aaaatagaag
     8821 aggtcgcaag aaaatgctac tgctggaaat actcgggata acgttgagta acagtttcct
     8881 gttctttcag catcatgtgt aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt
     8941 tatcaactaa agccttgagt aaatcataat gttgttccat gatcgtttcc catggaagcc
     9001 cctgaatggt cactcgtaac cagcggaaac gttctaaatg ggtattatgg tcatctaacc
     9061 agttccaaac ttcttttctt cctgcaattt cgaaacaagc ttcatgaaat aaattatctg
     9121 cacggaaaaa agcgcgttcg tcatttattt tgactgcttt ttcttgttct tctaaaatcg
     9181 tttctagcac tattcgtgtt tgtgtgtcca atttggaaca acattcaatc atgatttgct
     9241 cttccaactg ttctcggaca aagcgagcat tttctgcaga aacaagatta attttggaaa
     9301 catacgtacc actttgaggt atcgtataca cgagttcttg ctgacgtagt tgaatcaatg
     9361 cttcgcgaat gggagtccgg ccgatagcaa gatgttcctc cagtgccttt tcagatattt
     9421 tccttccagg ttctaagtca gcatagataa tttgtttacg aatcgcttga taagcttgtg
     9481 attgcaagct gcttttcatc tttattcctc caaaatcatt tctatgaacg tagtataaca
     9541 gaagaggtct gaaaaacaat ctggtatgtc acattaacta gtaaatttgt ataaatagta
     9601 aaattttggt tgacatagcg atttttattg cataaaattc acttgaatac aaaaactaat
     9661 atgttagttt aggaggggaa aaatgattga tttaaacaac tggaggaact ttatccctga
     9721 attatctcaa aaagaagtag aaatacctca ttttgaccgt gcatcattaa gagaaaatgg
     9781 acaaaaaaat cctgtctgga tccatttcgg tggcggaaat ctttatcgag gatttcatgc
     9841 agaaatcgct caacgtttag cggatctagg cgaattgtcc actggtgtca ttgtttgtga
     9901 aacattcgat gaagaaataa ttgaaagggc ttaccaagat tttgatgatt tgattcttga
     9961 agtggtcatg ccaagcagtg gagagttcgt caaacgtata ttagctgcga cagctgacag
    10021 tctgtattgt catccagatt gttctgaaca ctttgataag atgattcgac actttgaaaa
    10081 tcctagttta caattagtta cttttgctat tactgaaaaa ggttatcgat tgaaagatac
    10141 aaataataaa tatataggac cgattgcagt cgatataaaa aatggtccaa agaagccatc
    10201 acatacaatg agtattcttg ctgtgctact ttataaacgt tttttatctg gagcttggcc
    10261 gattgcactt gtctctacag ataatttttc acaaaacggt agtcaattac gtacttcttt
    10321 gctaacgatc gcaaaagaat ggcaaaagga aggttatgta caaaatacat ttatccaata
    10381 cttatcgaat gaagaaattg ttgcgtttcc atggacgatg attgatcgta tcaccccgaa
    10441 tccttccttg tcagttagca aaatgttggc aaaagagggc ttttcaaata tgtcgatttt
    10501 ccaaaccgca aaaggaaata atattgctcc atttgtcaat acagaggaca tccattattt
    10561 aatcatggaa gatcattttc ccaatggacg tccgaaattc gaaaaagctg gggtattctt
    10621 gactgatcgt gcaacggtag ataaagcaga tacgatgaag gtgacgactt gccttaatcc
    10681 tttgcatacg tctttagcta ttatgggttg tttactgggg tataggagca tagctgagga
    10741 gatgaaagat ccagatttat cgctgctagt ttctgaaatc gggtacaatg aaggcctccc
    10801 agttgtggag tctccaggta tcattgatcc aaagatcttt ctggaagaag taattactgt
    10861 gcgcttttct aatccaaata ttcctaatat gcctcagcga atcgccacgg atacctctca
    10921 aaaggtggcg attcgttatg gtgagacgat taaaaaatat atggaaatag aagttaaaga
    10981 cccgcagaca cttgtattca taccattgac tctcgctgct tggcttcgat accttattgg
    11041 cattgatgat caaggaaaac cattcatccc gagtccagac ccactattga cagaccttca
    11101 gcataagctt tccgatattc aactgggtac gcaagataca tcacatattc atgagaaaat
    11161 ggctcctatt ttgtcaaata aagaaatttt tggtgtagat ctttaccaag cagagcttgg
    11221 cagaaaaata gaagaaatgt tcatcaagat ggtagcagga ccaaatgccg tacgaaaaac
    11281 attacaggat tacttacaag aacatggagg aaatttatca tggaaatgac ttttagatgg
    11341 tacggatcac aggaagaaaa gattacctta gatgaaatca gacagattcc tgcggtcaaa
    11401 gggattgtag gcacattgat ggatatacca gtaggagagg tatggcctag ggagaggatc
    11461 aaagaactaa aagaagaaat tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc
    11521 aatgtgcatg atgccattaa aattggatta ccttcccgtg agcagtatat cgaaaactat
    11581 aagcagacaa tccgaaactt ggcagaatat ggaatagaag tcatctgcta taattttatg
    11641 cctgtatttg actgggtgaa atctgacctt gactaccgct tagaagacgg ctcatccacg
    11701 ttagctttta tatctgatga tattccagat gatccaagag aaatcgtcgc gcgcatcgaa
    11761 cattccagca atgaatttgg attgccggga tgggaacctg agcgcttagc tcctatcaaa
    11821 tccttgcttg aagcctatgc atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat
    11881 tttctccaat caatcatccc aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca
    11941 gatgatccgc catactcgat ttttggatta gctcgaatcg taaagaatcg agaagattta
    12001 gattggttgt gtcgcatagt agacagccct tataacggaa tcactctttg tacagggagt
    12061 attgcagaag atccagaaaa tgatgtatat gggattttag cagaattttg ccaaagagat
    12121 cgaatcccat ttgcacatgt tcgaaatatt aaatttttag ctaatcgaga tttctatgag
    12181 gcaccacata aatccgaata cggttcatta gacatgtacc gcatactaaa aacaatgtat
    12241 gacaacggat ttaacggata catccgtcct gatcatggaa gaatgatctg gaatgagaca
    12301 ggaagaccag gatacggctt atatgatcga gcattaggtg ctgtttatct aaatggatta
    12361 tgggaagcaa tcgaaaaagg aaacaaaagt aaagataatt agtgaaaaaa actgtaattt
    12421 tattatgtaa ataagaagga tgttcaaatt cttgatacat aaatccggag gttgttaata
    12481 agcattcgga tttatgtatc ttatttggtc taggaagtaa cttgattttc ttttttcgat
    12541 tgatccatga gtgcttcttc ttttaattca aagatatatt ctgagatcct cataatcact
    12601 tttttccagt cttctggtgt gatgattcct tttaaaatat agattttttt gatatgagaa
    12661 ggcaattgtt tcaaagggca gtcgctgata acgatgttgc agttctccat aaaatcattt
    12721 tgtattttga tcgattcatt tgaaaaaatt tgttctatct taacgatcag atctttactg
    12781 atataaaaat cacgcgtata gttcacacca atcacaattc gcggctgttt tttgtagcga
    12841 tcatacaaat gtactaggtc ttcaaccatc cattgcacat tttccttttc taaaataact
    12901 ggtaacaagt gttgattttg ttctctgaaa tccatataaa attgttcaat ggcggtttgg
    12961 acttctttca atacgctatg ttcttgataa agcatgaaag taggaagggg cgtatttggt
    13021 tgattgaagt taagaaatcc attataaagt ttatttcgtg cgaatgccaa tgagtaaaga
    13081 atcttttctt tttcaggtat aaacaaatca aatacagtac aaagggactc caccatttct
    13141 cttgaataaa gaacatgagg aacgtttgct ttttttaaaa gtttgtattg ctccaagctt
    13201 gtttcttccg attcgatttt cgtgatagaa agtcgagcta aaatattcac taagatgcgt
    13261 tgttcgcgac aaattggcag attaggtcgc aaaatatcta cagagagatc aataaaaaag
    13321 tcaatatatt catactctgt gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa
    13381 ttctctttac atatttttaa tagaaaatat aatttatcta tcataccgct atttagtcgg
    13441 tcaagtactt cttttttaaa aaaagtattc atcataaaag ccactacttg cggatcttca
    13501 tcataaaatt ctgtagcagt acttgaaaag atgttccaat aaatatctaa tgctgcatat
    13561 tgaatttgta cctcttttcc agaaaaagac tttctgccat tattagaaaa ggagatagac
    13621 aaaccgtact atgctaaaaa tgcattaatc tttttcattt tcgtatacaa ataagaggca
    13681 ctatagtttg tttgctgact gaaactgata atcgaatttg tctgacagct gaacaaagaa
    13741 gtcaagatct gataactgac tgattcgttg cagtatcttc caaatagacg ataatagcag
    13801 taagccggat caattccttc aaaattcaac tttaaaaaag gtttttgctt atcaatatgc
    13861 agttctgtta gattgagacg ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa
    13921 tttgattcag acatatggaa atgatctgct aaatattgtg tagtaataaa gaaacgatgt
    13981 tcagatagga aaagaaaaat atctaatttt gtattgcttt ttttcttcaa gataaacgtt
    14041 ttaatcgctt cttgagtttg catgaaagag gctccttttt acttaaattg ttaaaacttg
    14101 aaaatattac tgtttttttc ataaatttag acaaaaaaac agtaaacata taggtagaat
    14161 ctctactatc ttatttcttg catacaaaaa aatcaaggag tgagagagtt atgagatatt
    14221 caaccatctt aatatcaaca tttgcagcct taagcttatc gcttactata aatcagacag
    14281 tccacacaga ggagacagaa acagccaaat ggacagaaac caagtgggcc aacgtcaatc
    14341 gaattacctt tcaggatgaa ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag
    14401 gacaaacagc gaccatcaaa gcaaaaattg actattcggg agacttagca cctatactac
    14461 aatcagctgt tacatttgag aatgttgatc ccactttgtc gattggctat gacgagaaca
    14521 ctttgactat ccaacacaag caagcgatat ttaccttcac cgttacatta aatcaggact
    14581 tgttaaaacc agcgcttttt actgtcaagg tatcagatgg actaccgaac gatcatcatt
    14641 taacgccgta tagtcagcgg cagacaattg agcaagattc agccatcgac tcaggaggag
    14701 atcttgttga agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta
    14761 cagaaaatcc agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag
    14821 aaactcaacc agagccagac aataaatcaa aacctgaaat aacccctgga gaaaaaccag
    14881 atgtaactcc agaaccagat acggattctg gaaaccaaac agtaccggaa acaaatccag
    14941 acacagataa tgaaactgaa aatccagaaa aaccagaggt agatccagaa gaaaaaccag
    15001 atgtgactcc agaaccagat acggacgcaa gagaccaagg aataccagaa aaaataaaca
    15061 aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa
    15121 aaataaatga agagcagctg aataaaaaat cacgaatatt tgatagcgcc caatcagctg
    15181 aaaccttaaa aagtagcaag gatacaacct ttgctgtcct gaaacgaaaa ataaacagct
    15241 accaaaatcc ggagagtcac aaaacaaagt tatactgtgg agcggaatca ttctattaag
    15301 tatagcaaca atgctttctg caaaaagatt taaacaaaac agatctctct aacatttgaa
    15361 taatgtctgt gaacgcattt ttcaaataaa aagaaatatc cagaaaatga ccccaaaata
    15421 ccagattgaa atctgatatt tggggtcgtt catactttat ctttcttcat atttatttta
    15481 agggatatct gtttcttttg gaagatggtt cgggatcttt ttcactttca atacatctaa
    15541 aaagaaggta aagatcggaa tgccgacaat caagccccaa acgccaaaga accgttcgct
    15601 aacaagtaag accacaaatg tataaaaaat cggcagttct gttttgctgg acatcaactt
    15661 cggattcagc acataagact caattagatg gacaatcaaa atcgtaatca ggatataaac
    15721 aacatctcgg atgcctcctt gggaataagc tatgaaagat agaggaatac aagaaatgat
    15781 aacacctgct actgggatca gacttaaaat gaaaatcatg atcgctaaac tcagcagctg
    15841 atgaaagcca aatacagcca aggcgattgt agtgagcaac gtatttaaaa gagcgacaac
    15901 aaattgagct tctaatacca aaccaaatgt attgacaaac ttatcggcaa aatagtaaat
    15961 atcttgaaag aaccaagaaa attcactttt taaaaacaat cttgagaata caattgtttt
    16021 tttcttttca atcataaaaa agaaacttaa gatgaatgac atagcaaacg ccactgccat
    16081 ttttcctata tcctgtaaat agccaagcgc aatcgaagcg ccattttgaa gctgttcaaa
    16141 aaaatcgttt cgctcaatat aagtatggat atattgtaaa acaggatctg catcttttgg
    16201 ctgatgctcg taaaaattaa taacggactg gatcatgtca aatgtttgat gaataagcat
    16261 cgggatatac tttgtgaatg ccagatagat aaggtaaacg atcagtgtat aaatcatgat
    16321 agctaaaaca cctgtaggga ttcggacaaa tcgctgaatg atttgaacaa gatgaatcat
    16381 caaataagta aagataaatg tgagcaaaat ggttgtaatc atacttctaa ctaagaaaag
    16441 aacaaaaacg acaaataata aaacagtgag acggcgtatt ttttcattcg ccaaaaaacg
    16501 ctgataaacg gtcaaatcga caacctccaa ataacaaaaa ataaacacgt tattcattat
    16561 atgatggaat atgaaaataa gaaagagcaa aaagaaaaac cgctaaatga cgttatctta
    16621 tgagttggtt ttcctcataa aatcgttcat ctagcagttt cagccctttt tctggtcttg
    16681 atgaaaatta tctagcattt ttcgggtcag atgttgcagt gtatccaatt cttcctgttc
    16741 aagtggaaga taagacttga tcttttgtgg aataccttct gcttgttttt gcaacgcagc
    16801 accttgagca gtcagcgtaa catttacgat tctttcatca cccgtttcac gctgacgatt
    16861 aatataattt ttttctgcta acttttttag taagggagtc agcgtaccag aatccagaca
    16921 caaatcattt cctagctctt tcactgaagt ctttttcttt tcccataaaa ctaacatcac
    16981 aagatactgg gtataggtaa gattcagcgg cttgaggagg ggagtgtagt gtctaatgat
    17041 ttctttagag gcggcatata aaggaaaaca tacttgatta actaaatata gatcttccat
    17101 ttttccacct ccgctgtttt actatagtgt atcatagcgg atttacacaa aaaagtctat
    17161 tcactaactg ctttcagagt aacttctata tttccagctg tagcttttga atacggacac
    17221 actttatgcg ctgcctctaa taattcttgc gtttcttcca gagaaagacc ttcgatagat
    17281 ccttcaatct ctacacctaa gacaaagctc gtttcacttt ctgaataaag ggaaacttgt
    17341 gcactgacag tagaagcccc agagatattt ttttgctgtt tgatcaattc taatgcactg
    17401 ttaaaacatg cactgaaacc agctgcaaac aattgttcag ggttggttgc attttcgacg
    17461 cgtttaccag gagctgcaat attcaattcg aatgtacgat cgggattatg cacttctcca
    17521 gagcgtccgc ctgtgttgat cattttcgta gaatacattt ttttcataaa attaccacct
    17581 ttaattatat tttatacaat tgaattttac acaatataat tgtggaaagc aaagaaatgg
    17641 attggcataa gataaataag agaaagcaca tgaaacagac gcgctttctc gaatagcaaa
    17701 catatacaaa aaatatcaga gacaaaaaat aatctcatca taaacattac atatatttct
    17761 caaaaagttg ttttctttct tcaaaaatta cttcacgatg gacctgatga agcatccata
    17821 aataaccaaa aggatctctg aacattgagt tagtcacacc aaagtccatc atttctgtca
    17881 tttcttgaat ctctacacag ccatttcgta atgctttatt atgtgtgtcc tcgatatcag
    17941 agacgatgat attgaaccag atcgtatttg gatgttcagc agtaggagca gtcaaaccaa
    18001 gagacgtgtt ttcatccagc atatggaatc tcgtaccata caaggtaaaa atgacttcat
    18061 tattgcctct agggtaatct gttgcctcaa tcagttgtat ttcatcaaag attgtttgat
    18121 acagttctag tgctttcaaa ctatcattta atacaaaatc gatttccgtt cctttatgca
    18181 taaagatctc ctctcctttt cagtcgaaca gttactagat tcttttatta taccagctaa
    18241 agaaaacgca ttcaaaaaaa tagcggattt acttaccaac tcattcgatt ggatgataag
    18301 taaatccgcg tcccattact tctgctacgc tgctgacaat gacaaaggca cttggatcga
    18361 tttcttctat caatggtttc attcgggaaa attcatgatc agtcacaacg atcaataaaa
    18421 cctcttttga ctttcgatta tacccgcctt ttgcctctag cagggtaagg ccacgtccaa
    18481 tctcagcact taagcgaagg cggatgtctt ccaaataatg ttcactcatg accataacag
    18541 atttctttcg attgatacct gtttcgatat aagtcatgac aattgaagtg attgcaatag
    18601 ataaaatagc ataaagaaac tcttctattc caaaaataaa cagattaaga gaaacaacga
    18661 atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg gaaatatttt ttaaaaatga
    18721 ggggagggat cgtcgtaccg ccgctagaag agttgttttt atataaaatc gcaactccta
    18781 aagcaaaaat cgcacttcca aaaatgacag acaacaaccg atctgaagta atcattgttt
    18841 ctggaactat tgcaatgatt aaaggaaaaa ccaagcttcc gaaaagtact ttaaaaaagg
    18901 gttttttacc taaaaataga agagctagta ccaacataac gatattcaac actaaaataa
    18961 ctatagctcg gtcaaagcct aaagcagctt ccagtaatat tcctagaccg ctgacacctc
    19021 cagcagcaat atgatgaggc cctaaaaaca tattgatagc gactcctaat atgaagaggg
    19081 caaaaataat cttaatgtat gtccatataa attgtctcgt ttctttcatt tattcacttc
    19141 cttaattcat taataatact cttatcttat tataacttac ttttcacctt aagttaactt
    19201 taattaataa aaaaattagg ctagtctaaa ttattatgtt tcattcttct agaatatact
    19261 tctacgccaa aaactgttaa aaaactttta ctttagaaaa ttggagaaat tgatcgctta
    19321 agataaattc aggacactaa atagtacagg aagtagacta aaacttttaa aagttttaag
    19381 tctatctatt tggcaagttt aaaaaataaa aattcggtat caaaaaactg ttaccgataa
    19441 catttttgaa agcgttgtct tttgcttctc tatcacttat agtgatgacg tcatattaaa
    19501 acacaaggag gaaatcaatg gatacagctg caatttatca tcgaccggaa agtgaatatg
    19561 catttctata tacgaataca agatttcgta ttcggcttcg tactaggaaa aatgatatca
    19621 aaaaagtata tgtgctatgt ggagatccat ataccataac aactgaaaaa tggtatcaaa
    19681 aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca tgattattgg gaaattgaag
    19741 tgacgagcga aacaagtcgc ttacagtatg cgttccatgt tgttggggaa gataacacgg
    19801 actgttttta cggtgatcaa ggaatttttc cttatcgaga aaatgtgatt actgaaccaa
    19861 atttttattt tcgaatacct tattttcatc aaatcgatcg attcactatt ccagaatggg
    19921 taaaagaaac agtatggtat caaatttttc ccgaaagatt tgcaaacggg gacaaagcaa
    19981 atgaccccga acacacttta ccttggggaa gtaaagatcc agaccgagaa gactttttcg
    20041 ggggagattt gcaaggtgtg atggatcatt tggattactt gacggacctt ggcatcaatg
    20101 gcatctattt ttgtcccata tttaaagcca catccaatca taaatacgat acgattgatt
    20161 attatgaggt ggatcccgct tttggcgata aaaaactttt aaaaaacttg attgatgaag
    20221 cacataaaag agggatacgt atcatgttcg atgctgtatt caatcatatg ggtgtccatt
    20281 caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc gatctataaa gactggttcc
    20341 acatccgttt tttcccagta gattcttatc agatgactga cttaccagaa actgctgaaa
    20401 atatcccata tgatactttt gcttttactc cattcatgcc gaaactaaat acagcaaatc
    20461 cagaagtgca aaaatatttg ctggatatcg caacttattg gataaaagaa ttcgatattg
    20521 atggatggcg tcttgatgtt gctaatgaag tcgatcatca tttttggaaa aaattcagag
    20581 aagcagttac agaaatcaaa ccagacatct atatccttgg agagatttgg cattcctctc
    20641 aagcttggct gcaaggggat gaattccatg cagtgatgaa ttatgcattt acagattcga
    20701 ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca aatggtcagt ggaatgaatc
    20761 atcagcaaat gctttacaga gatcaggtga acgaaggaac attcaactta ttagattctc
    20821 atgatactgc caggatcttg accctttgcc aaggtaacaa agaattgatg aagtctgtga
    20881 tggcctttat gtttcttcaa aaagggtctc cttgtatcta ttacgggaca gaaataggga
    20941 tgactggtga agatgacccg gattgccgca aatgtatgat atgggagaag gaggaacaag
    21001 atctagagct attcggtttt ataaaagaac ttgtttctct aaggaaacag ttgtctaaga
    21061 ttatttctga aggttctact cagtggctaa tagtaaatga tcgtgaagat aagctctatt
    21121 tcacacggaa actagaggga cagatcatct atgtttactt taatcagtca aaagaacctt
    21181 gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa ttgtcagttg ttagaagacg
    21241 ggaaagcaga gatacgacaa tacggatttt tgatcatgtg ttgaaaactg ttaccggtaa
    21301 caacaagaaa gcgtttgcaa atgaagattt gtgcccatta taataagggt gtaacagcac
    21361 gttgaataca aaggaggaaa tgaagatgaa aaaaagaagt gtgaaacagt tgggtttagg
    21421 gttaatctct ttaggaatct taggcggatt agcggcttgt ggcaatggta actcttcagc
    21481 agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc gttgattctg gatataaaga
    21541 ttatataaac gaaataaaaa acactttcga aaaagaaaac gatgtgaaaa tcaagctagt
    21601 agaaaaggac atgttcgatc agttggaatc attagcgtta gatgggcctg ctggaaaggg
    21661 acctgacgtt atgatggcag catatgaccg gatcggtgcg ttaggacagc aaggacatct
    21721 ggcggaagta aaactgggaa atgaagcagc ttacgatgaa acagacaaag cccaagtgac
    21781 ttatgatgga aaaatctacg gagaacctgc agtaatcgag acccttgttt tgtactataa
    21841 caaagattta gtcgatacag caccatcaac gtttaaagat ttggagaatc tatccaaaga
    21901 cagtcgtttt gcttttgagt cagaagcagg aaaaaataca ggatttttag ctaaatggac
    21961 tgacttttat tattcctatg gtttaattgc aggttacggt ggctatgtat ttggtgatga
    22021 tggcacaaat ccttcagata tcggtttgaa taatgcaggt gctgtagaag gaatctctta
    22081 tgcaacagat tggttcaaaa atgtttggcc aaagggaatg caagacataa aaagtgcagg
    22141 agacttcgcc agccagcaat tcatgtccaa taaaactgca gcaatcattg acggtccatg
    22201 gcaagcgcaa acatacaaag aaaacaatat caattatggt gtagccaaaa ttcctactct
    22261 aaataatgga cagccttatc aaccgtttgg cggaggaaaa ggatgggtag tcagcaatta
    22321 tgctaaaaac aaagacctct ctcaaaaatg gcttgattat gtaacgaatc aggaaaatca
    22381 agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac caacaagcta gggaaacagc
    22441 aaaggctaaa aacgatgaat taacaacagc tgtgattgaa caatatgaaa cagctcaagc
    22501 gatgccaaac atcccagaaa tgggcgaagt ttggacaggt gcagaaaact tgatgttcga
    22561 tgcagcatca ggttcaaaaa caccaaaaga atccgcagat gaagcagtaa aaacaatcag
    22621 tgaagccata gaacaaaaat actgattaaa atgataaccg tccattttta taaaaaatgg
    22681 aacaggtgct tcagataaaa gtggcaagag cgagatggaa aattatcttg ctcttgttgc
    22741 cattatctta aaagcagttt gcggtttagc aagtttagag ggggcattca gatgcagcaa
    22801 gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt gttcacttat tccagggatg
    22861 gggcagtttg taaatggcca agcttttaaa ggtatcgttt ttttcgccat attcgtccta
    22921 ttcattggtc agatgcttct gggcggattt catgcattgg taggattggt tactttggga
    22981 agtataccaa tggaagatca ttcattattt attttgattg aaggcacttt gcaattcatc
    23041 attacaatca ttttcttgat tttttacggt ttgaatcttt tagacgctta tcgagtagcg
    23101 aagatgcgtc aagagggcaa aaaaatcaat cggacgatga aggaagtcat acataatata
    23161 ggtgatgaag gttttcctta tctattgaca cttcccgctt atcttctcat gatttttacg
    23221 atcattttcc cagtcctagt tacattgttc actgcattta ctaattatga cttcaatcat
    23281 attccaccag ccagtttaat tgattgggta ggggctaaaa acttttttag tatcttcttt
    23341 ttaagctcat atcgtaatac tttttttgca gtcttttcgt ggaccgtgat ttggacttta
    23401 tgtgcaacaa ctttgcaaat cacccttggt attctcacag cggttgttgc tcaccagaaa
    23461 tttattaaat tcaaacgtat tttcggtatc attttcttat taccatgggc ggtcccagct
    23521 tttattacta tcatgagttt ttcgaatata ttcaacgata gcgtaggtgc catcaatact
    23581 caagtgattc ctttcataaa ccagcttcct tttgtagata tcagtgcaat ctcttggaaa
    23641 acggacccga actggacgaa agtagcaatt atcttagtgc agggctggtt aggatttccc
    23701 tatatctatg tgatggttac aggaatctta caatcgattt cagaagactt atatgaagca
    23761 gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa acatcacgct tcctgctgtc
    23821 ttcctagtgg cagcgccaac atttattaca cagtatacgg ggaatttcaa taatttctcc
    23881 atgatctatc tgtttaacaa tggcggtccc ggaagcgtag gcggaggagc tggttctact
    23941 gatattctta tttcctggat ctataaactt acgactggaa cttctccgca gtattctatg
    24001 gcttcagcaa tcacgttgat tatctccgtc atcgttatct gtgtgtcatt gctggtattt
    24061 aaaaagacaa atgcgttcaa tatgggggat taacaatgaa aaaatttcat tcgagtgctc
    24121 gattccaaaa acgactttct cactttttta cttacttttt tcttttaata ctgtcagtca
    24181 ttatcatcta tccgcttttg atcacagcaa gtactgcctt caagtcagga aatattgcag
    24241 cttttagttt agatctttca agtgactgga ctttaggaaa ctttactcgg ctattcagtg
    24301 aaacacttta cgggacttgg tataaaaata cattgatcat ttctgtccta acaatgatcg
    24361 tacaagtgac tgtagtaact ttggcgggct atacttatag tcgctatcgt tttatcggcc
    24421 gcaaatcaag tttgatgttc tttttagtga ttcagatggt accgactatg gcagcattga
    24481 ctgcctttta tgtcatggct ttcttattag aagcgctcga taagtactgg ttcttgacaa
    24541 tgatctatat aggcggtgga attccaatga atacttggct gatgaaagga tactttgaca
    24601 ctgtaccaat agatttagat gaatctgcta aacttgatgg ggcagggcat ttcaggattt
    24661 ttttccagat tgtattaccg cttgtcaaac cgatgctcgc cgttcaagca ttatgggctt
    24721 ttatgggacc ttttggggat ttcatgttag ctaagttcct gcttcgaaca cctgaaaatc
    24781 agactgttgc tgtcggctta caaacattta tttctgatgt gacaaaccaa agagtctctc
    24841 tttttgctgc tggggcaatt ttgattgcac taccgatttg tctattattc ttctttttac
    24901 aaaagaactt tgttaccggc ctgacatctg gcggaacaaa aggataggag cagagtatca
    24961 tgcaaacatt tccgttaaac tatttttccg cattacgtac gccaactcaa gtgtttgcgg
    25021 gacgcaaact attatcatgg cctaaatttt tcttgatatt tgtattttta gtttccttga
    25081 tggtcatgcc tgtgactctt ttttatgcca atcagatcca agcgattcca ttagaacaat
    25141 ttctatccgt tcattcgctg atcgatgaac aaggaaccca aaaattttct gaacttgaat
    25201 tatctgaaac tgggttacaa gcatcgcaac aaacgatagc tgtgacccct gaaattttgg
    25261 taggtgtgtc tttatctgag aaacaacaat ccgatcatgg aacttttatt gattttgaga
    25321 aggaacaatg ggtgatccaa caaaaggata aatctggtat acgccgctat acgatgaatt
    25381 atagcccatc tttccaacca gattctgtac gtacgcccga agatttccaa cgatttttag
    25441 aaagagaatt ttatgccagt aatcgtccaa caatcatttt atcttatagt ttaagtcttg
    25501 gtcttgtcct atttgtcatg acgagtctca ttttattcgg ggcttccttt ttcttgtgga
    25561 tgacaagaaa aagccaactt tcatccattc atacattcaa agagagtgca aatttgatgc
    25621 tgaatgttat gggaataggt tccatggcag cagcagttgt agggtttatc cactttgact
    25681 ttatcttgat gctaagtgtc caaacaacga taactgtctt gctgctttta tgggtttttg
    25741 tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt ttaagcgatt tatgcgacaa
    25801 tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa atttctgaaa aaattaaact
    25861 tcttagttga ttcttttctt ttttagtatt aacgtaatag ggtagttttt ttaaggagga
    25921 aaatcatgtt agagaaaact ttatacaatc aattattttc acgttctttc tcgctccctg
    25981 tcgaagtaaa ttattgggac ggtacaacga aacaatacgg agatacggac actcctcccc
    26041 aaatcaaaat caatatccat gaagaaatcc caatgaaaga gattacaagt aacgcatctt
    26101 tggcactagg agaagcctat atggatcatc gaatcgagat cgagggaagt atccaagcat
    26161 tgatcaacga tgcttatcag caagcaaaca gctttttacg aggaaaagat tatctaaaat
    26221 ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa agaagatatc catgcgcatt
    26281 atgacttagg taacgacttt tataaacttt ggctagatcc aactttgact tattcttgtg
    26341 cttatttcaa tacacctgaa gatacgttgg aacaagcaca aatcaataaa gtccatcata
    26401 tcttagacaa actctttatc aaagaaggcg aaacgttgct tgatatcggt tgtggctggg
    26461 gaacattgat gttcactgct accaaagagt ataatgtcaa ggcaactggg atcacactaa
    26521 gtgaagaaca atacgatttg attaccaaaa gaataaaaga agaacattta gaagataaat
    26581 gccgtgtatt attaatggat taccgcgaac taaaaggcga aacgtttgat catatcacaa
    26641 gtgtaggtat gttcgaacat gtaggttctg aaaatcttga aggttatttc aaagtggtaa
    26701 aagatttatt gaaaccaaaa ggaacagctt tgatccatgg aatcagccga caacaaggcg
    26761 gtgcgacaaa tgcttggatc aataaatata ttttccctgg cggttatgtc cctggtgtta
    26821 ctgaattagt tgataatatt acaaaaaacg atttgcaact gattgatttg gaaagcttac
    26881 gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc
    26941 aagtcacgaa agaaatgggt gattccttct atcgaatgtg ggatctatat ttacaagctt
    27001 gtgcagcttc atttgaatca agtaatatcg atgtgattca atatttgctt gttcaacctg
    27061 gaaacaatga catcccaatg catcgtgcat aattgaggca aaagtactgt ttaaagacta
    27121 tttaaaaaga agctaagttt tatgatgctc cagaagtgaa cttttttgtc taacttctag
    27181 gattgtctct aaagcttagt ttctttggct ctttgtcaaa taggactgat agagtgagat
    27241 aagaacatct aaggtagaat tccttttcta ctttagatgt tttttgattc ataggtaatc
    27301 agattattta gtaagaaaat tctggtcttc attttccgaa agcttcgaaa gccataagcg
    27361 actcgtttca atgctttgat gtgggtatgc agattttcta ttttggcatt tgaataaggc
    27421 aattcgaggg ctaaacgaat gccggattca tagtttataa aagctttttt tatagaatgc
    27481 agttcttttg gaacggaatt gggcatcgtt cttacaagtt taaagaaagt gtccgcctct
    27541 ttgtcgtgaa acgcttgaag aatttcttga tagtaaaaat aggcgattcg taattcgtca
    27601 gagatcgaaa gcagacgatc caccacttca ctttcggtga gatacgccca accaaaattg
    27661 cgccgtttgg tccatttgtc ataaacaagc ttgtcctgtc gtttgatcag aagccgagac
    27721 aacgccttta attgtcgata atatttggct tgtgttggat cgtttcgatc aaagcttttc
    27781 atgattcgta cgcgaatatc ctcaaattta cgaatgagat gtttgacaat gtgaaaacga
    27841 tccacaacga gttttgcctt tggaaaacat tcctttatca gagaggcata agaaacattc
    27901 atgtccgtca cgatgacctt taccttcaat cgtgcgcgtc gttcaaattg cataaaataa
    27961 gaaacgagag cctttttctt ccgtgaagga agaatatcca gtaattttcc agtatcgcca
    28021 tccatacaag aaaaggagta gggaccgact tgatttttca gtgaacgaaa ttcatccatt
    28081 aataaacaac gcggtaacca atttcttttt acttttgtca tggggatcag agaagcgagt
    28141 gtccgagaaa cggtcgacca agaaacgccg caatgcttag caattgtcgt cattgcttgt
    28201 ttttcagtta gctcttctaa aattttatag cgcacttgtt tagcgataaa gcagtttcta
    28261 tcgacaatgt aggtattcgc cgtaaaatag gtgtggcagc cacgacagtg gtagcgttgt
    28321 ttcgctaaag caagataagt cagttggttc gcacatttat taagttgaat gagcgatttt
    28381 ttctttccgt tcttaacgat catgccttca tgattcatct gtttacacaa aggacagcga
    28441 cgaatttctt tacttaacgt cgcagaaaca acaagactct tgatattctt aatgctcttc
    28501 gattccacat ttgtgatgaa aatgttttta tctttgattc caagtagtag tttgatatga
    28561 tgaagatcag gcatacagaa cgctcctttg attgtgttgt ggtgattcaa ttctaacgga
    28621 g
//
LOCUS       DACEOE010000016        28546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEOE010000016 DACEOE010000000
VERSION     DACEOE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.016"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            227..715
                     /gene="purE"
                     /locus_tag="IW774_000628"
     CDS             227..715
                     /gene="purE"
                     /locus_tag="IW774_000628"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1153002.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            699..1832
                     /gene="purK"
                     /locus_tag="IW774_000629"
     CDS             699..1832
                     /gene="purK"
                     /locus_tag="IW774_000629"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1153003.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1845..3140
                     /gene="purB"
                     /locus_tag="IW774_000630"
     CDS             1845..3140
                     /gene="purB"
                     /locus_tag="IW774_000630"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1153004.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3320..3697)
                     /locus_tag="IW774_000631"
     CDS             complement(3320..3697)
                     /locus_tag="IW774_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153005.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            3953..4681
                     /locus_tag="IW774_000632"
     CDS             3953..4681
                     /locus_tag="IW774_000632"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1153006.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            4681..4935
                     /gene="purS"
                     /locus_tag="IW774_000633"
     CDS             4681..4935
                     /gene="purS"
                     /locus_tag="IW774_000633"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1153007.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            4937..5608
                     /gene="purQ"
                     /locus_tag="IW774_000634"
     CDS             4937..5608
                     /gene="purQ"
                     /locus_tag="IW774_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1153008.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            5609..7831
                     /gene="purL"
                     /locus_tag="IW774_000635"
     CDS             5609..7831
                     /gene="purL"
                     /locus_tag="IW774_000635"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1153009.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            7816..9255
                     /locus_tag="IW774_000636"
     CDS             7816..9255
                     /locus_tag="IW774_000636"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1153010.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            9287..10330
                     /locus_tag="IW774_000637"
     CDS             9287..10330
                     /locus_tag="IW774_000637"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1153011.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            10327..10905
                     /gene="purN"
                     /locus_tag="IW774_000638"
     CDS             10327..10905
                     /gene="purN"
                     /locus_tag="IW774_000638"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1153012.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            10902..12437
                     /gene="purH"
                     /locus_tag="IW774_000639"
     CDS             10902..12437
                     /gene="purH"
                     /locus_tag="IW774_000639"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1153013.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            12453..13727
                     /gene="purD"
                     /locus_tag="IW774_000640"
     CDS             12453..13727
                     /gene="purD"
                     /locus_tag="IW774_000640"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1153014.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            13849..14763
                     /locus_tag="IW774_000641"
     CDS             13849..14763
                     /locus_tag="IW774_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1153015.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(14896..16461)
                     /locus_tag="IW774_000642"
     CDS             complement(14896..16461)
                     /locus_tag="IW774_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1153016.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(16454..17905)
                     /locus_tag="IW774_000643"
     CDS             complement(16454..17905)
                     /locus_tag="IW774_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1153017.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(17919..18935)
                     /locus_tag="IW774_000644"
     CDS             complement(17919..18935)
                     /locus_tag="IW774_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1153018.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            19155..20243
                     /locus_tag="IW774_000645"
     CDS             19155..20243
                     /locus_tag="IW774_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1153019.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            20297..21823
                     /locus_tag="IW774_000646"
     CDS             20297..21823
                     /locus_tag="IW774_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153020.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            21825..23030
                     /locus_tag="IW774_000647"
     CDS             21825..23030
                     /locus_tag="IW774_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1153021.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            23287..24264
                     /gene="guaC"
                     /locus_tag="IW774_000648"
     CDS             23287..24264
                     /gene="guaC"
                     /locus_tag="IW774_000648"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1153022.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            24528..25952
                     /locus_tag="IW774_000649"
     CDS             24528..25952
                     /locus_tag="IW774_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1153023.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            26413..27522
                     /gene="pdhA"
                     /locus_tag="IW774_000650"
     CDS             26413..27522
                     /gene="pdhA"
                     /locus_tag="IW774_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1153024.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            27525..28502
                     /locus_tag="IW774_000651"
     CDS             27525..28502
                     /locus_tag="IW774_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1153025.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         9221 a         4877 c         6447 g         8001 t
ORIGIN      
        1 agcttctcat gtttgttgaa tattgttcct tgtgaccgag gagttggcag gagaacaccg
       61 tttatccata aaaaggaatg tgacctaact tttgtcacat tccttttttt tatctttaat
      121 aaacacgaac aataaaatgt ttttctttgt taacattcga aatttctttg acatcaaaaa
      181 aagtgattgt taaaatatga atgtagaaaa actgggagga aaaacaatga cagcaaaaat
      241 ctctgtgatt atggggagta cttcagattg ggaaacaatg aaagaagcat gtcaaatatt
      301 ggatgaattc ggtgtgtctt atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt
      361 gatgtttgaa tatgcacaaa acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc
      421 agggggagcc gcgcatctgc cgggtatggt cgcatcaaaa acaactttgc cggttatcgg
      481 tgtaccggtc caatcacgaa cattaaatgg tttggattcg cttctttcga ttgtccaaat
      541 gcctggagga gtacctgtag ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg
      601 actacttgcg atacagatgc tttccatgta tgatttagaa ttggaggcta aattggctga
      661 aagacgttca atgttggcta aaactgttat agaaagtagt gatcaacttg gttaaacctt
      721 tgttgcctgg ttcaacgatc gggatcgtcg gtggcggtca attaggtcgt atgatggcga
      781 ttagtgcaaa agaaatgggc tttaaagtag gtgtgctgga tcctgtgagc gattgtccag
      841 ccgcacaagt tgcagattgg cacattgaaa gtgtctatga tgacaccttt gcgctgaaag
      901 agcttgccag aagaacagat gtcattacct atgaattcga aaacgtcagt gtagaggcgt
      961 taaatgcgat tttacccatg tcatttattc ctcaagggac agacttgctt gcgattacgc
     1021 aagaccgcct gttagaaaaa tcttttttgg aaacgaataa catcgtgatt gctccttatg
     1081 caacgatcgt cagtccgacg gatatccaag atgcgattga cgggatcggc tatccttgtg
     1141 ttttgaaaac cactcgaggc ggctatgatg gcaaggggca gtatgttttg aaaagtcggg
     1201 cggatctagc tcctgccatg gaccttttaa gagaaggaac ctgtgtttta gaagcatgga
     1261 tcccgtttga aaaagagatt tcgatcatgg tggcaggaaa tggtcaagga gatttcacga
     1321 cttttccggt cgtggaaaac atccaccaca acaatattct tcatgaaacg attgctccag
     1381 ccgcgattga tcaggatgtg atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag
     1441 cagtgagcct ttcaggtgtt ttaggggtag aaatgttttt aacaaaaaca ggcgggctat
     1501 atgtgaatga attagctcct cgtcctcaca attctggaca ttattctatt gaagcttgtt
     1561 ctatgagtca gttcgatgcc catattcgag gtatttgtgg ttggccatta ggagaaaaag
     1621 tacgtttgat aagcaatgca gtaatggtca atgttttggg agaacaactg ctggaaagct
     1681 atcgcttgat tgagaataag ccagactggc aattccattt ctatggaaaa aaacaagcaa
     1741 aaaaaggtcg caagatggga catatcacta tcttgacaga agacatccag caaacgctgg
     1801 aagaaatcaa acaaacgaat atttgggatt agaggagaaa aaacatgtta gaacgttata
     1861 cacgccctga aatggggaat atctggacgg aagagaacaa ataccgtgcg tggctcgaag
     1921 tcgagatttt agcagatgaa gcttgggcag aacttggtga aatcccgcaa gaagatgtga
     1981 aaaaaattcg tgagcatgca tcatttgata tcgatcggat cttggaaatc gaaaaattaa
     2041 cacgccatga tgtcgttgcg tttacacgag cggtttctga aacattggga gaagaaagaa
     2101 aatgggtcca ttatggtcta acaagtacgg atgtcgtaga tactgcttat ggctatctca
     2161 tcaagcaagc aaatgatatt ttacgggaag acttgaaacg ttttacggaa atcattggcg
     2221 aaaaagcaaa agaacacaaa tatacagtga tgatgggtcg gacacacggt gtccatgctg
     2281 aaccaactac ttttggattg aaacttgcca catggtattc agaaatgaaa cggaacatcg
     2341 aacgatttga acatgcggca aaaggagtag aagcaggaaa aatctcagga gcagtgggaa
     2401 cgttcgcaaa tatcccgcca tttattgaag agtatgtatg taaacaattg gggatccgtc
     2461 cacaagaaat ttctactcaa gttctccctc gggatctaca tgctgaatat ttctcagcga
     2521 tggcgcttat tgctacaagt attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat
     2581 ccgaaacacg tgaagtagaa gaattttttg cgaaaggtca aaaaggttct tctgctatgc
     2641 cgcacaaaag gaatccgatc ggttcagaaa atatggctgg tctagccaga gtggtgcgcg
     2701 gacacatggt cactgcctat gaaaatgtca cattatggca tgaaagagat atttctcatt
     2761 cttcagcaga acgaatcatc ttgcctgata ccacgatttt attgaattac atgttaaatc
     2821 gttttggcaa tatcgttaaa aatctaacgg tctttcctga aaacatgaag cggaatatgt
     2881 ctgcaacatt tggtttgatt tatagccagc gcgttatgct gaaattgatc gataagggaa
     2941 tgacaagaga agaagcttat gatcttgttc agccaaaaac tgcgtacgct tgggacaacc
     3001 aagtgcagtt ccgcccgctg ttagaagaag atccaaccat cacatcggta ttatctaaag
     3061 aagaaataga cgatgccttt gattatcatc atcatttgaa aaacgttgac gtgatattca
     3121 aacgagtagg tttggaataa agaagagtga gttgcaaaat tcaattaaaa tagaatagca
     3181 aaaataacca aaaaagaagc agatccgtta gcggttctgc ttcttttagt ttattatatt
     3241 ttcttatcaa taataggaac ctgattttaa aagcggctac ctctttaccg tgcctcttta
     3301 catacaaaaa atagaggaat taagcgtaaa tggcttggct attataacta aaatgctggt
     3361 tatggagatg ttgcagcaag ttttcgagaa aagcgtattc catctcttgt ccattgaata
     3421 agatctggat acgataatga tttttgaaac gcttctgttc atgaatctga ctgattgaaa
     3481 gttcgccttt gtgttctgtc tgataaagaa aagtcctttt acggcattca gcacagcgct
     3541 tggttgctcg gcggattttt cggaatgtat aaaaactagt atctttattg cataaataaa
     3601 cgtctttgat cgacagcaaa tcattatata gacaggaacg ttcttcagct gttaattctg
     3661 ataaaaaaag ctgaccttgt tttattgcat cttccatcgg caagtgcctc ctcgaaccca
     3721 ttatccaaat ttttagttaa ttctaatata ccatcattaa gtataaatgt aaacgcttgt
     3781 tgatggtata agattttcgt ttctgataaa acacgaacat taaaagaatc taatgcctta
     3841 aatgtacata tttttgttga tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac
     3901 gaacaaaaaa cgatgtattt gcttttaatg taaataaaaa ggagagacgt acatggagaa
     3961 gaaagaactt ttgtatgaag gaaaagccaa aaaaatatat cttacttctg atgagaactt
     4021 gatttggata gaatatttag atcaagcaac tgcgttgaat ggcgcgagga aagaccagat
     4081 aagtggaaaa ggagaattga ataatcaaat cacaagtgca attttccgtt atctgactca
     4141 aagaggaata aaaaatcatt ttattgaaca attgtctaaa accgaacaat tagcaaaaaa
     4201 agtacaaatt atccctctgg aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa
     4261 acgtctaggt gttcccgaag gtaaagaatt catcaaacca atcattgaat tctactataa
     4321 ggatgaccag ttggatgatc catttatcaa tgaagagcat atctatttct tacaacttgc
     4381 aaagccggaa gaaatcagtg agctgaaagc ccaagcgttg gctattaatg aaattttgac
     4441 cgaattattt gctaaggtcg gtttggtttt agtagatttc aagctagaat ttggccggtc
     4501 gaatggacaa attttgcttg cggatgaagt aacaccggat acttgccgat tatgggatga
     4561 acgtacgaat gaacatatgg acaaagacgt ttaccgcaga gatctaggtg atttgatccc
     4621 agtctatcaa gaagtatata accgtctatc ggaactaact gaagaaaagg agcgagtatg
     4681 atgtatttcg taaaagtata tgtaacctat aaagattcag tgttagatcc tcaaggggag
     4741 gcagtaaaaa aagcagtcca tcgtatggga tatgaaacaa tcgaagatat ccgaatagga
     4801 aaatactttg agatacaact tgctgatgca gaagaaccaa tcgatacagt gattgaaacg
     4861 atttgtgaca agctgcttgc aaatgtaaat atggaaacat accgatatga aatcaaaaaa
     4921 atggaggaag tataacatga aatttgcggt catcgttttt ccaggttcaa attgtgacgc
     4981 agatatgtta tgggcagttc gcgagatcat gggcgctgat gccgaatttg tacggcatga
     5041 tgagtcctct ttagaaggat ttgatggggt tttactgccg ggaggattct cctatggtga
     5101 ctatttgaga tgcggtgcga ttgctcggtt ttctacaatc atggaagaag tgattcgttt
     5161 tgctgaagaa gggaaaccag tattcggtac atgcaatgga ttccagatat tgacagaagc
     5221 aggacttttg ccaggtgtac tgagacgaaa tgagtctttg cattttattt gcaaaactgt
     5281 accattgaaa gtcaacccca agacggcctt tacttcagag tatgaagccg acgaaataat
     5341 ctatcttcca gttgctcatg gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga
     5401 actaaaaaaa aataatcaga ttgcctttac ttataatgac aacatcaacg gcagcgtgga
     5461 aaacattgca gggattacga acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga
     5521 acgtgcagta gaagacttgc taggatcaga agatgggaaa cggttctttg cttccatttt
     5581 aaaaaacttc ggaaaggtga ccgtatagat gaaaacattg aaagaaccga cagctgaaga
     5641 gatcaagaaa aaaagaatct atgccgaatg gggattgact gatgaagaat atcgaatgat
     5701 cgaagaagat atcttaggaa gaatgcctaa ttatacggaa actggactgt tttctgttat
     5761 gtggagcgaa cattgttcct ataaaaattc taaaccggtt ctacgcaaat ttcctactag
     5821 tggacctcat gtgttgcaag gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg
     5881 actagcagtt gtcttcaaag cagaaagtca caatcatcct tcagccgtgg agccttatga
     5941 aggagctgcg acaggagttg gcggaatcat cagagatatt ttcagcatgg gggctcgtcc
     6001 aattgcgttg ctagattcat tgaggttcgg cgaattagat aatccaagaa cgaaatattt
     6061 actagaagaa gtagttgcag gtatcggcgg atacggcaat tgtatcggca taccgactgt
     6121 aggcggcgaa attgcatttg atccttgtta tgaaggaaac ccattagtca atgcaatgtg
     6181 cgtaggtttg attgaacaca aacatatcca gaaagggcaa gccgctggcg tcggtaattc
     6241 gatcatgtat gtaggtgcaa aaacaggtcg cgacggtatc cacggagcaa cttttgcctc
     6301 agaagaattt aatcaagaag aagaacagca acgttctgct gtacaagtag gcgatccttt
     6361 tatggaaaag ctgctgcttg aagcgtgttt ggaattaatc ttgcatcatt cggattctct
     6421 aataggtata caggatatgg gagcagcagg acttgtttca tctagtgcgg aaatggcttc
     6481 aaaagctggc tcaggactta tattgacatt agatgaagtc ccacaaagag aaactgggat
     6541 gacaccttat gaaatgatgt tatcggaatc acaagaacgg atgttgattt gtgttaaatc
     6601 aggagaggaa gaaaaaatcc aagaactatt tcagaagtat gatctagatg cagtcactat
     6661 cggtaaagta acagacgacg ggcaatatcg gctatatcat caaggagaag aagtagcaaa
     6721 tctgccagtc gatgcattag ctgaagatgc accagtttat cataaagaaa tgaaagaacc
     6781 agctcgaatc gtagaatttc agcagttagc tccttatcag ccagttatcg aagaacctgg
     6841 acaagtattg cttgatctgc tgcagcaacc gacgattgct tcaaaacgtt caatctacga
     6901 aacttatgat tcacaagtac aaaccaatac agttgtccgt ccaggaagcg atgctgctgt
     6961 gatgagggtt agagggacga ataaagcgtt agctatgacc acagactgca acgcgcgcta
     7021 tctgtatttg gatccaaaaa tcggaggaca aatagcagtg gcagaagctg ctagaaatat
     7081 tattgctagc ggtggtaagc ccttggcgat caccgactgc ctgaactatg gttctcctga
     7141 taaaccagaa gttttttggg aattatcgac ttcagctgac ggaattgctg ctgcgtgtga
     7201 aacgttagga actcctgtta tttcaggaaa tgtctcttta tataatgaaa cggatggtca
     7261 agcaatctat ccaacaccaa tgattggtat ggtcggattg attgaggatc ataaacatat
     7321 cactactcag gaattcaaaa aatcagggga tctaatctat atattaggta agacgtttgc
     7381 agattttgat ggcagtgaat tacaaaagat gcagcttgga agaatcgaag gtgtgatccg
     7441 aaactttgat ctatccattg aaaagagaaa ccaagagtta gtattgacag ccatccaaaa
     7501 cggattgatt gaaagtgctc atgactgttc agaaggtggc ttggcaattg cactggcaga
     7561 atcagctttc aaacatcagc tagggatatc tgtacagttt gaattgtcct ctgctcaatt
     7621 atttgcagaa actcaatcaa gatttgtgtt gacagtcgca ccggaaaata agacacgatt
     7681 cgaagaaatg atgggagatg ctgctgtttt agcaggtaag gtgacggatg aagcgataat
     7741 cgaaatttca gcaactgacg gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg
     7801 ggaggatgca atcgtatgtc ttatgaagtg aagagtttaa atgaagaatg cgggatcttc
     7861 ggtgtttggg gtcatccaga cgccgctagt gtggcatact tcgggctcca tagtcttcag
     7921 catcgaggac aagaaggtgc aggtatcgtg tccaatgatc gaggaaagct taaggggcat
     7981 cgagatttgg ggctgctttc tgaggtattc aaagatcaaa gaaaattgca gcagttaaca
     8041 ggagaagctg caatcggaca tgtacgttat gcaacagcag gcaatggaag tgtggacaat
     8101 attcagcctt ttctattcaa atttcatgat cagtcgatcg gcttagcgca taatggtaac
     8161 ttgatcaatg ctcgttccct tagaaaagag ttagaggaaa atggggcgat cttccattct
     8221 aactccgata cggagatatt gatgcatttg atccgacgaa gtgaagaaac agatttttta
     8281 aataaattaa aaagtgcttt gaatgaagtc aaaggtggct ttgcctattt attaatgaca
     8341 gaaacggcaa tgattgccgc tttggaccca aatggttttc gtccattagc aatcggacaa
     8401 atgaaaaacg gagcatacgt gattgcctcg gaaacttgtg cattagaagt tattggggca
     8461 cgttttataa gagatgtcca gcctggtgag atagtgatca tcaatgacga aggaatccag
     8521 attgattgct ttacgaagga cacgcagctt tctatttgtt cgatggaata tatctatttt
     8581 gccagaccag attcaaacat cgcagggatc aatgtgcata ctgcacggaa aaatatggga
     8641 cggaatttag cgaaagaatc gccagtaaaa gcagatatgg tcgttggcgt acctaattct
     8701 tctttgtcag ccgcaagcgg ttatgcggaa gctagtggaa tcccttatga aatcggatta
     8761 gtaaaaaatc aatacattgc ccgaacattt atccagccaa cgcctgagct aagagagcaa
     8821 ggtgttcgaa tgaaattatc tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg
     8881 gtcgatgatt caattgttcg gggtacgaca agcagacgga tcgtccatct tttgaaagaa
     8941 gcaggggcaa aagaagtcca tgtgaggatt gcttctcctc ctttgaaata tccatgtttt
     9001 tacgggattg atattcaaac gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa
     9061 atcaaagaat gtatcggtgc agattcttta ggttttttaa gtgaagaggg tctgatcgaa
     9121 tcgatcggtc tggaaaaaga tgctccgtat tcaggattgt gtatggctta tttcaacgga
     9181 gattatccga cgccgttata tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa
     9241 gtatcgttct tttaaaagca gaccaagaag ggagtatatg aattcaatgg caaatgcata
     9301 cgcaaaagct ggcgtagatg tcgaagctgg atatgaagta gtagaacgga tcaagaagca
     9361 tgtcaaaaaa acggaacgtt taggtatgat gggcgctata ggaggattcg gcggttgttt
     9421 tgatttatcg gtctttcaat taaaagagcc agtccttgtc tcggggaccg atggcgtggg
     9481 gacgaaatta gcagtggcaa tcgaagcaga caaacacgat acaatcggga tcgactgcgt
     9541 agcgatgtgt gtcaatgata tcttagcaca aggcgcagag ccgttatatt tcttagacta
     9601 cttagcaacg gggaaaaatg atccgttacg tttggaaaat gtagtaaaag gtgtagcaga
     9661 tggatgtgtt caaggaaata tggcgctgat tggcggagaa acagctgaaa tgcctggtat
     9721 gtactcaggt aatgattatg atctagccgg atttgctgta gggatagctg aaaaatctca
     9781 attattgaca ggagaaacga tcaacccagg agatgtgttg cttggaatag catctagcgg
     9841 gattcattca aatgggtact ctcttgttcg caaaattttc tttgagcagc agcagttttc
     9901 tttatcatca aaactgccag aattgaaaaa aacacttggc gaagaattgc ttgagccaac
     9961 gaaaatctat gtttcttcct tgcttccgct gatcaaagaa aagaaaataa aaggagctgc
    10021 acatatcact ggtggagggt ttgtagaaaa tatcccgcga atgttaccag aatctatggc
    10081 tgcacaaatc aaacttggct catggccgat tcttccaata ttcggctgtt tacaaaaata
    10141 tggtgaaatt cctgagatgg agatgtatga aatcttcaat atgggcatcg gtatggtcct
    10201 ggcagtcgaa gaatcgaaag taaaagaaat agtacacgaa attgagcaag ctggcgaaaa
    10261 agtttatgaa atcgggaaag tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga
    10321 agaacgatga gaatcgctgt ttttgcttct ggaaacggaa gcaactttca agcgctagcc
    10381 gattatctat cgaaaaaagg attggaatct tcgattgact ggttgttttg tgatcagcct
    10441 gaagcatatg ttttgaaacg tgcaactgcg ttgagtgtac cggcagattg tttttcacct
    10501 aaagaatttg attcaaaaaa agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa
    10561 aagatcgatt tgatcgtatt agctggatat atgcgaatca ttggaccggt attgctgaaa
    10621 aattatgata aaagaatcat caatatccat ccgtctcttt tacctgcctt ccctggtttg
    10681 catggcatac gtgatgcctt tgaagctgga gtaaaagaaa cgggtgtcac aattcattat
    10741 atcgaccaag gtgtagatac tggaccaatc atccggcagg aaaaagtgag aattgaacag
    10801 gaggacacgt tcgattcttt agaagaaaaa atccatagag tagagcatcg aatctatcca
    10861 gaagtgatta gtgaaattat tgaaaatgga ggctatctga aatgacaaga gcattgatca
    10921 gtgtatcaga taaaactggg attgtagcat tcgcccaagg tttgattgaa caaggagtag
    10981 aaatcatttc aacaggagga acaaagcatg cgttggataa agctgggatc aaaacgcttt
    11041 cgatcgaaga agtgacaggg tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc
    11101 ctaaaatcca tggtggtctt ttaggaagac gcgatctccc tagccatatg gaggcaatgg
    11161 aaacggaaaa tatccaacca atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa
    11221 cgattatgaa accagatgtc gaaatagcag aagctattga aaacattgat atcggtggtc
    11281 ctagtatgct ccgtagtgca gcaaaaaacc atgaatttgt cacctctgtt gttgatccat
    11341 ctgactatga agaagtactt tcagaattac gtcagtatgg gcatacttca ctttctttca
    11401 gacaaaaact agcggcaaaa gtctttcgtc atactgcggc atatgatgca ctgatcgctc
    11461 aatacttaac ggattgggtg gaagaaaaag aaccggaaaa attgacactt acgtatgaac
    11521 gaaaacaaac gcttcgttac ggtgaaaata gccatcaagc cgctgccttt tatcagtccg
    11581 ctcttctgga aaaatattcg atcgcagcag caaaacaact ccatgggaaa gaactttctt
    11641 ataataatat ccgagatgca gatgctgcgc tacggatcat gaaggaatat gacgagccaa
    11701 cggtagtcgc cttgaaacac atgaatcctt gtggtattgg gacagcatct acgattttag
    11761 aagcgtggga agcagcgtat gaagcagatc ctgtttcaat ctttggtggg atcatcgttt
    11821 tgaatcgaga agtagatctg ccaacagcag aacagatgca taagctgttt ctagaaatca
    11881 tcattgcccc ttcttataca caagaagctt tggaattact gcaaaaaaag aaaaacatcc
    11941 gattactgac agtagacttt gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag
    12001 tcttaggtgg acttcttgta caagatcaag atctggcaat agaaaatgaa gaagagtgga
    12061 aagtcgtgac aaaacgtaag ccgactccag cagaagcaaa agcattagtg tttgcttgga
    12121 gagctgtcaa acatgtgaaa tctaatgcga tcgtactggc aaacgaacgt cagacagtag
    12181 gaatcggagc cgggcagatg aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc
    12241 aagagaaaat ggaaggtgca gttttagcta gcgatgctta ttttccaatg gatgattcgg
    12301 ttgaatatgc agccaaacat ggtataaaag caatcattca gccaggagga agcatcaaag
    12361 accaagattc tattgatatg gcgaataaat acgatctagc aatggtcttt actgatgttc
    12421 ggcattttag acattagaga ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg
    12481 gtcgtgaaca tgcaataagt aaaaaacttt tagccagtcc gaaggtggat acggtgtatt
    12541 gtgcgccagg aaatccgggg atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc
    12601 gagatcattc agcgttaatc cattttgtta agaaagaggc tattgattgg agtatcgttg
    12661 gtcctgaagt tcctttgtta aatgggatcg tcgatgattt tatggcggaa ggattgaaaa
    12721 tcttcggtcc gacaaaagca gcagctctga tcgaaggttc gaaaagtttt gctaaacaat
    12781 taatggatac ttatcaaatc ccgacagcta aatccagaag ttttgaatgt tacgaaaaag
    12841 cagcagcgta tatcaaagaa aatggtgcgc caatcgtagt gaaagcggac ggcttagcag
    12901 ctgggaaagg tgtagtcgtg gctaccacag tgaaggaagc gctggatgcg gcagaacaaa
    12961 tgttgaacca gcatcgattt ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg
    13021 caggagaaga attctccctt ttggcgtttg tccgtaatac caaggtgtat ccgatggtca
    13081 tttcgcaaga tcataagcgg gcatttgata acgataaagg gccaaataca ggcggaatgg
    13141 gtgcttattc accagtaccg caaatttcaa aaaagatgat tgaaaaagct gtggaagaag
    13201 ttttaaaacc agcggctttg ggaatgaaag aactaggacg ctctttcacg ggtgttttat
    13261 atgcgggttt gattgctacg aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg
    13321 gtgatcctga aacgcaagtc gttctttctc ggttgaagag tgactttgcg caagtaatcg
    13381 atgatttatt agagaatcgt acagtagagt taaagtggca gcaagaagga tacaatgtag
    13441 gcgttgtggt tgcagctgcc ggttaccccg aagaatatga gacaggaatg gttctaccag
    13501 attttctgga agaagagctt tctgtttact atgctggagt ttcctcaaat aacggcaaac
    13561 tagttggagc aggaggacgt ctttatttag tagaagcata cggtgagacg atcaaagaag
    13621 cacagaaaaa aatttacacc gcgttaatgg ataaaacgac agatggaaca ttttatcgta
    13681 cagatattgg agaaaaagct gccaatagcg aactgatcgt gaagtaaatc agctgcttcc
    13741 atttataaaa aatacaacaa acataatcgg aagcgccgat tttagtgaag ccttttcata
    13801 aagagtgtgg tacacttttg ttgtgtatat aaataaggag gagttcagat gaaaaaaatt
    13861 caattaggta cttctgatca gaaagcagct gcagtgatct taggctgtat gcgtatcaac
    13921 aatgcggaag atccagtaaa agtgattgag acagcttatg aaaacggcat tgattttttt
    13981 gaccatgcag atatttatgg aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca
    14041 aaaacatcaa tcaaacgaga agatcttttt attcaaacaa aatgtggaat cgttccaggt
    14101 gtgatgtttg atttttctaa aaaacatatc attgaatctg tagaaaaaag tttgacccgt
    14161 ctgaaaatgg attatgtgga tgctttactt cttcatcgtc cagatacatt ggtagaacca
    14221 gaggaagtag ctgaagcgtt tgatgaattg gaacggtcag gaaaagttcg ttattttggc
    14281 gtcagcaatc aaaaaccaat gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta
    14341 gtagctaatc agctgcaatt tggcttgaaa catactggaa tgatcgatca agggattcat
    14401 gtcaatatga cagatgaagc aagtatcgat catgacggta gtgtacttga ttattcccga
    14461 ttgcatgata tgacgatcca agcatggtca ccataccagt atggattttt tgaaggtgta
    14521 tttatcggga acgaaaaatt ccctgaatta aaccaaacat tagaagaact agcagctaaa
    14581 tatcaaacga caccaactgg cttggcttct gcttggattt tacgccatcc agccaatatg
    14641 caagtgatcg ccggtacgat gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa
    14701 attaatatgt ctcgggaaga ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct
    14761 taaagattgt cataagcagg tcaaaagggc tataaattaa aaagacagaa taacttgaaa
    14821 gctgttttca gcttcgagat attctgtctt tttttattgc aaatggaaag agtatcattg
    14881 tacggatttc gtttattagt tatttttgtt tttaaggaaa agctgtgtcc gatattcttt
    14941 tggagaaatc ccttgttttt tcttaaataa ataactgaaa tagttagggt cattataacc
    15001 aatttcaaaa gcaatatctg aaatacgata gtctgattgc tgcaataatt tttttgctaa
    15061 ttcgattctt tgttctgtca gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat
    15121 cgtactgaaa tgagagggac ttaatgcaac ttgttgagcg acccaagtta aggacatatc
    15181 tggatcggta aaattttctt tgataaaatc caatgcatgg ttcaaaattg gctgatattt
    15241 gttccttgaa ggctgtgagg gcagagcagt taagaaagaa atgaacatat aaactgtcga
    15301 acggtaaagc gagtcgtcgg cgctgattgc taagcgttga ctggtggatg tgcaatgaac
    15361 catttcttgc gggattttct tttctttcag gcagacatgc tggagtagct tagtcaagac
    15421 aaataaacga tataatcgtg ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat
    15481 gtacttagat acttcgtttt tcgataatga agacaaatca agtgagaggt caaagtaaaa
    15541 gacagaatca actggaagtt tcgcttcttg aatcaaatcc tcatagctga taatttgttc
    15601 tttaagggaa ctggcggttt caagtatcat ctgcgtgatc tcaaaagatg caggtaattc
    15661 gctgattctt tctgcgacag gaccaatggc gacagaaaaa ttattttttt gtcccttttc
    15721 taattcatgg accaacgttt gggctacctg ataactggtc tcgatcacat tttcttcaag
    15781 agaatcaact attaaaaatt tgatgaatcg agaggaaaca gcagagaaga tcacttgttg
    15841 attctcccta aataaaaact tcaaataatc actgagatga gcgtaatctt caaagtctgt
    15901 actgacttgg tttgctgcta ataatacggt catttttttc cctgctaaag agcgattgaa
    15961 agattcagca agtttcaatg tttcactgat cgtaagtgag cctttgaaca aaccgttcaa
    16021 gaaatgattt tttttcaatt cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg
    16081 caaatctaaa gcagttactg ctttttctat tgcttgtttc aattcgctac ttttaatcgg
    16141 tttaagcaga taatcatctg cctgtacctg gattgctcct ttcatataat cgaaatcgtc
    16201 gaatccacta ataaatatga gacgagtcca aggaagtagt ttccttgctt cttttgaaaa
    16261 agtcaacccg tccatgaaag gcatttggat atcagtcaga atgatgtcgg gctgtaagtc
    16321 gataattgaa gacagtgcca tctcaccatc tccagcttcg ccgacataag tgattggata
    16381 ataagaacca aggacagtta tttgttcccg caagttattg cgaatgaggt attcgtcttc
    16441 gatgataaaa actttataca tgtttgtttc ctccaattgt tttgggagat agagggatca
    16501 tcagtgtgac agttgttcct tctcggtatt tgctgtcaat cgtgataggt ttttgatcat
    16561 ttccgtaata aagaaggaga cgcttgttta cgttgtaaag tccgtagccg atactgaaat
    16621 ctgaatcgat ccctttggct aactctttac gtatttctgc cagtttttct gcagggatgc
    16681 caatcccgtt gtctataact ttgaattgga tcatctcatc aattttgcat gtggtaatac
    16741 gaataaatcc tttgtgacgg acgtacttcg ttccatggta tacagcattt tcaatcaatg
    16801 gctgcaagat catttttaaa atagaaacag gaaaaagtgt ttctggtata tcgatcttat
    16861 agtcaagcat atggccgtat cttattttta agatgactag ataatcattg atatggctaa
    16921 tttctttttc aagaggaatc cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg
    16981 aaagtgcatg ggtcatagtc agagcttctt caaagcagtc ttgttcgatc aaagaaataa
    17041 tggcatccaa actgttataa ataaaatggg gagtaatctg agcttgcaat acacgaactt
    17101 cactttgtgc taaatggtat tgtttcaaag cgttctcccg taaagactgg tttagatctt
    17161 ccgccatttt attcatgcta gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta
    17221 aacgaaagcc aaggttccct gaagaaagtt gttctgacat ctcgatcagc tgattgatcg
    17281 gttcttggac ctttctctct aaaaagcgac gattcttttt cgcatagaaa aaaatggtac
    17341 taagaactcc aagttccact attgttaata gaatgagcga gcggacaagt gtcgtatttt
    17401 ttttgcttgc taagtcgatc tccacacgaa caaaatcttg taagatatct gacagcagtt
    17461 gagtgacaga atctacctga ttgatgatct cttcattggt atccataaaa tcttttgcca
    17521 agatattatt gatcagctcg ttttgataat cttccagtgt atcgatcgta cgtagcgata
    17581 catctaacgt actaatttct ttaagtgtat ttgtattatt tttgatctct cgaatatcat
    17641 tcctaagttc ttctaggata ttttcctcat tgaactcatc tacggttact tggccagaaa
    17701 tcaagtccca catactttcc aaaacttttt gatcaacctt caaagcaatt gcatctgctt
    17761 tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc
    17821 tcagtagcaa agggataaca gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct
    17881 gttttatgct taaatttttt gtcatgatac ttctccagtc aataatttct ggttgggatg
    17941 ttagctatat tctgctggga aaagacagtc tcggaaataa aaatatcttt aggtaatgtt
    18001 tttcctgttt gaatatttcc aaaataacga gtaacggtat tggctacata ccagccgaca
    18061 tcaggtttac actcgactac acaattgacg attcctgagc gtagcttctc gatcatatct
    18121 tcttgtccat caatagttac aatgatcaaa tcttctccag gtttgattga tgtttctttt
    18181 atcgcagcta aagcaccgtg agtcatttca tcactatggc tgtataatac atcgatttcg
    18241 gataaatcat ttgtttctaa atatcgtttc atctggtctt ttcctttcat ccgaataaaa
    18301 tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat ggtcgatggt tccatctttt
    18361 tttagggttg gtttacgatt gattgattcc aaaaatcctt cggagcgtag cttggtcggt
    18421 gtggagttct ctgaaccagc aagttctagg attctgattt cctgtttctt tgaatttgtg
    18481 aagtgattgc tgacaaataa acccgcccga tttccttctg ctttgaaact ggggccaatg
    18541 tgggtgacga aaagctcggg atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg
    18601 atccctgctc gctttgcttc ttttaataca gagtcccacc cggattcttg caaaggagta
    18661 aaaatgatca tatccacttt ataggcaatg aacgtccgta tatcttggat ttgccgttcc
    18721 tgattcatgt agccatttcg gtagagaact tggtaatctt ctttttcaag cgcagtgata
    18781 attgattcag tatgcttttt ccgccagcta ctctccgttc ctgattgcga aaaaccaacg
    18841 actagtgggt caggtgcttc ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt
    18901 acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa atcactccat ctggatttgt
    18961 tagtactatc ttatcagata aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa
    19021 tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat
    19081 cccataaaaa aatcaatgtt aacgctttct ttattagcta tactcggtgt atcaattgaa
    19141 atgggggata aaaaatgaaa aacaaattta gaaagattgt tgggactatc gctattttga
    19201 ccgcaagcat tctattaagt gcgtgtggca attcaggatc tgcagatgat agtacggggt
    19261 atgtaggaat cgctatgccg accaaatcag cagaaagatg gatcgctgat gggaacaaca
    19321 tggtctcgga attagaaaaa ctgggttata aaacagatct tcaatatgga gaagataaag
    19381 tagaaaatca agtagcacaa atagaaaata tgattacaaa aggggtagat acacttgtga
    19441 ttgcttctat cgatggttcg gctttaactg atgtgttagc taaagcaaaa gaagcagata
    19501 tcaaggtgat cgcctatgat cgtttactga tgaattcaga aaatgtcgat tattacgcga
    19561 cttttgataa ttttggagta ggtgtatcgc aagcagctta tattgaagag cacttaggat
    19621 tgaaagaagg aaaaggacca ttcacgattg aattatttgg tggctcgcct gacgacaaca
    19681 acgcactaat caattacaac ggagtgatgt ctgttttaca accatatatg gacaacggtc
    19741 agttggtcgt gccatctgga cagacgagtt tcagtcaaat cgctacgctt cgctgggatg
    19801 gttctacggc gcaagcaagg atggataatc tgttaagcgc aaattatact gaccaaacat
    19861 tggatgctgt tttgtcgcca tatgatccaa taagtttagg aatcatttcg tcattaaaag
    19921 gcgtggggta tgggagtgaa agtaaacctt tacctgtgat tactggacaa gatgcgacag
    19981 ttgctggcgt taaatcgatc attgccgggg aacaaacaca aacgatcttt aaagacacac
    20041 gtatcttagc aaaaaatacg attgaaatga tcaaagcaat cagtgatgga gaagaggttc
    20101 cggtcaatga tactgaaact tatgataatg gggtgaaaac tgtccctacg tatttagcaa
    20161 atacagtatc tgtcgataaa gataattatc aagcagagct gatcgacacc gattattata
    20221 aagaatccga tctgaaaaat taagaggaac caaacaagga actaaagaga tctaagtcag
    20281 agagaggata agaattatgg cggattgtat tttaaaaatg gcagatatca ccaaaaaatt
    20341 ttctggaatc cgagcgttag attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc
    20401 attgtgcgga gaaaacggag cgggaaaatc aacattgatg aatgtcttga gcggcttgta
    20461 tccatatggc tcctatgacg gggaaatctt ttatgatgga aagctgtgtc aatttaaaga
    20521 tttgaaagat agtgaagaaa aaggcatcgt catcattcat caagaattag cactaagtcc
    20581 ttatctttca ataaaagaga atatttttct cggaaatgaa caagcagaaa gaggcgtgat
    20641 tgattgggaa ctgacagaaa agaaaacata tgaactgctg aataaagtcg gtttgtctat
    20701 cgacccgaat cttctagtat cccagattgg cgtagggcaa cagcaactag ttgaaatagc
    20761 taaagcatta tcaaaatccg tccgtctgtt gattttggat gagcctaccg ctgcattgaa
    20821 tgaagaagaa agcgcgaatt tgctgagatt gatccgagag ttgaaagagc aaggtgtaac
    20881 ctccatcatt atttcacata agctgaatga aatcgtggat atcgcagatc gaatcaccat
    20941 tttacgggat ggacagacga ttgagacact agaaaatgaa atgatcagcg aagagcatat
    21001 tattcgcgga atggtgggca gagatttagt tgatcgttat cctgaaagaa aaccggatat
    21061 tggagaagtt tattttaaag tagaagactg gacggtctat caccctcttg atcacgagcg
    21121 aatcataaac gaccacatca acttgcattt gaaaaaagga gagattgtag gaatagctgg
    21181 actgatggga gcgggcagaa ctgaattcgc tatgagtcta tttggacatt cttatggaag
    21241 caagatatcc ggacgtatat taaaggaagg ggcagagata tcagttaaga ccgttccttc
    21301 agccattgca aacggactcg cttatgtctc ggaggaccga aaatctttag gactgaatct
    21361 gctgatggat attcgggaaa acgtgacgct gagcagtttg gataaaataa gtcaaaaagg
    21421 agtattagat aaagaaaaag aagtaactga ggcagaaaat ttccggaaaa agatgcgtat
    21481 aaaaacaaac tccatccatc aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt
    21541 cgttttatcc aagtggctga tgacacaacc cgatattctt tttttggatg agccaacccg
    21601 tgggatagat gttggggcaa aatatgagat ctacacgatt atcgaagaaa tggcagcaaa
    21661 tggcaaagct gtctgtctta tctcttctga acttcctgaa atattaggga tgtgtgatcg
    21721 aatctatacg atgaacgaag gacgtattac aggagaagtc tcgcgagaag atgcagatca
    21781 ggaagtcttg atgaaattaa tgacgaaaga agaggtgggt taagttggaa caggcaaaaa
    21841 atcttacgga taaaaaagag aaaaaactaa atttgaaaag tagagcagct gatctttttg
    21901 gtcgctacag catggtgatc attttagcgg gactacttat tgcgtttcaa ttgatgacgg
    21961 atggtatttt ctggcgtcca ttgaatataa ctaaccttgt tctgcaaaat agccatatat
    22021 tagtactagc tgcagggatg ttattagttg tgctcttagg tcatgtggat ttgtcggtag
    22081 ggtcagtgat ggcgtttgtt ggtgcaattg cgggcatgat gatggtcaat cgccaaatga
    22141 acccgtggat gacagttatc gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag
    22201 gtttttgggt agcttatgca gggataccgg cgtttatcgt gactttagca ggattgctga
    22261 tgtttcgtgg actgacacaa gttgttctag gaggccaatc tctagcacct tttccagctg
    22321 ttttccaaaa aatgtctacg ggttatttac cagacttaag cgaaggaagc agccttcact
    22381 tcttgacatt gattttagga atgatcatca cgatcgtgtt agttgttggt caatggcgaa
    22441 caagaaatcg tcggaaaaat catttatttg aagtggaatc tccaacagct ttcttggtaa
    22501 aggcggtatt tacagcagtc gttgtactag gaataactta tatctttgcc tcttatcaag
    22561 ggtatccgat tattttgatc attcttggtt tgatcgttgc agcatatggg tttttgacaa
    22621 ataaaacagt agcaggtcga cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac
    22681 tatcagggat caaaacaaag cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg
    22741 cagctttagc tggattagtt ttagctgctc gattgaatgc ggcaacacct caagcaggta
    22801 catcgatgga actggatgca atggcggcag tatattttgg tggtgcttcg acttccggcg
    22861 gcattggaac aatcatgggt gcgattgtcg gtggcttggt catgggtgta ttgaacaatg
    22921 gcatgtcgat tttaggcgtc ggggtagact ggcagcaggc aatcaaagga ttgatcttgc
    22981 ttttagccgt tgtgctagac ttgtataata agaaaaaacg aacaacgtag gagaacgccg
    23041 gttattcaga aaaaagactg tgacaagaca tttgtcacag tctttttttc gctaaaagcg
    23101 aacattcatt gtttcgtttg tatagactaa aaataatata tgttttttta ccattcgtat
    23161 aaattctctt tttgctcttg atttttttgc ttaattgagt taaattacat agggcgctaa
    23221 aaaaagcctt gattaaatta ataatgttcg tgttttgctg gatgtgaaca tcaggaggat
    23281 aatgtaatga aagtatttga ttacgaagat attcaattaa ttcctaataa atgtatcgta
    23341 aatagccgtt ctgaatgtga tacaacggtc acattaggta aacatacatt caaaatgcca
    23401 gtagtgcctg ccaacatgca gacgattatt gatgaaacaa tcgcagaatt cttagcggaa
    23461 aatggttatt tctatatcat gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa
    23521 aaaatgaaaa agcgtggatt gattacctcg atcagtgttg gcgtcaagaa agaggaatat
    23581 tcctttatag agaagttggc agaagaatcg ctaaatcctg attatatcac tatcgacatc
    23641 gcgcatggac atgctaactc agttattgat atgattcagc atattaaaaa atatttgcct
    23701 gaaacttttg tgattgccgg taatgttggt acaccggaag ccgtccgtga attggaaaat
    23761 gcgggcgcag atgcaacaaa agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc
    23821 aagacaggct ttgggactgg aggctggcaa ctggcagctc ttagatggtg tgcgaaagca
    23881 gctcgcaagc cgatcatcgc tgacggtggg atcagaacac atggcgatat tgcaaaatct
    23941 gtccgttttg gtgcgacaat ggtcatgatt ggttcattgt ttgccggtca cgaggaatct
    24001 cctggcgaaa caaaagtcga aaatggaatc gtgtataaag aatactttgg ttctgcttca
    24061 gaattccaaa aaggcgaaaa acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa
    24121 ggatctttga aagatacact agtggaaatg caacaagacc ttcaatcatc tatttcttat
    24181 gctggaggaa gagatctgga agccattcga aaagttgact atgtcatcgt gaaaaattcg
    24241 atttttaatg gagacacaat ctaaaaaata aataaaatat taggatgttg tgaaaaagtt
    24301 gtcctgcatt aagtaataag aacagccaaa caaaaatagt tctttatatg ttttgtttgg
    24361 ctggaattat tatcgcagat acaagctttt gaacatcatt attcagaata agggactgtg
    24421 ataagatttt tgtcacagtc ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa
    24481 attcggtaaa atcaaaagaa aaaatagaca atagaaaaga gaagcttatg tgggaaaaag
    24541 tagagaaaaa acaaggaccg atttatcgtc aaattatgga acagattatg agagaaatcg
    24601 aaaacggaag gatcaatcct ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt
    24661 ttggtgtgaa tcgttccacc gttgtccatg cattggaaga gcttagggag cttggggtac
    24721 taacgagcaa acgtggcagc ggatggtttg ttaatcagac tgaatggggg aaattcgctg
    24781 ttccaagaat cgattggcgg caaatgatct ctcctcgtta tgagcagacg gactggtatg
    24841 agcaaaaaat aagagaagca aagcagatcc aaaaaacatc gtttcttgat ttatacgcta
    24901 gcgagatgcc agatgagctt ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa
    24961 tcttagcgga agaaaaagaa atggatcttt taggctatac tccgttacgt aacaaaatca
    25021 aacggtattt ggaaagtaaa tttgaattgg cattttcccc caaccagctt cttgtgactg
    25081 gtggcggtca gcaagcaatc tttttgattt tacaaacgat tctttcagct ggagagggga
    25141 tagctgtaga gtcaccttct tttttttacc gattacctct tttcaaagct tcaggaacgc
    25201 gtttatttgg gataccgatg gatgaagaag ggatcgactt agaaaaactg aaagaatcga
    25261 tccataaaaa taaattgaaa gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa
    25321 aagtgatgag tcaaaaacga cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc
    25381 caattattga agacgatgta tttacggatc tttcatttct aaaccaagaa gaagtccttc
    25441 ctatacgaac gattgatccg gataatgtgt tatacgtcgg ttcgttatct agggttttag
    25501 gaaaaacaac gaaaatcggt tgggtgatag gacctgagac ttttatctcg caactagctc
    25561 aagcgcaaga aatgatggag ttttcgatga atatccttac acaaatcaca gtggctaatg
    25621 tatttaatga agcaattgat ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa
    25681 gccgactgat aagcgcttgg ggagattcac aatcgttttt caagcttcat tcaattttag
    25741 gtggttacta tgcctggatt acttgggatg ggaaaaaact agaccccgaa ctagcagaaa
    25801 aagtagtaga tatgggtctt ggaattgcac caagtctact ttttgggcaa gatataaatg
    25861 gaatcagaat caattacagt aggctggatg aaaaccaaat accgttgttt tattcaaaga
    25921 tgaaaattct agaaaagtgg ttaacagaat aatttcacaa agtaaataga ttaaagaata
    25981 taacagcttg gatttaaatt tcaagctgtt tctttttttt cggaaaaaga atacgatata
    26041 aagaaactaa gaaaaaacca ttatttgtac gtccgttcac gatgtgtaaa taaaataaat
    26101 gtttgcaaca aaactgtata acaccaacgt ttttaaatag aaacagatgg ttgtaagttt
    26161 gattagaaaa tagaaaaaat gcagatgaaa tataaaacgc tttccttatt gtgattttat
    26221 tcactaatgc tgttaatatg cggattggca taatttcctt tcatcgtgaa aatttgtgct
    26281 tttagcttgt atttctcata aatccatgat aatttattaa tgaagacggt taaatagtga
    26341 tacagtttta ttcactgttt caatcgcaga atgaagagat tttcttatag atgagagagg
    26401 aatgtgtgac aaatggcaaa caaaaaagca ccgatcgact tccaagcact gctagatgaa
    26461 gtcaatgcag atttcccagt atatcaagca cttgatcaag atggaaacgt agttaacaaa
    26521 gatttagtac ctgatttatc cgatgatgaa ctcgttgaat taatgactca tatggtatgg
    26581 tctcgtgtat tagaccaacg ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt
    26641 gcaccaactg ctggacaaga ggcaagtcag ctggctagtg cttacgcatt tgataaagaa
    26701 gatgttcttc ttccaggtta tcgggatgta cctcaactag taaaacatgg tttaccttta
    26761 tctcaagctt ttttatggtc tcgaggacat gctgcaggta attcttatcc ggaagaattg
    26821 aaagctctac caccacaaat tatcatcggt gcacaatatg tacaagcagc cggtgttgca
    26881 ttaggtttga agaaacgaaa caagaaaaat gtcgtcttca cttatactgg tgatggcggt
    26941 tcatcacaag gtgattttta cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc
    27001 gtattttata tccaaaacaa cgggtatgcg atctctacac ctcgtgaaaa acaaactgca
    27061 gcaaaaacac ttgcacaaaa agctgtcgca gctgggattc ctggtattca agtcgatgga
    27121 atggatccgc ttgcagtata tactgtatct aaaatggcac gggaatgggc gatcagtgga
    27181 aatggaccag tcctaatcga aacactgact tatcgttatg gaccacatac actatctggt
    27241 gacgatccaa cccgctaccg tacaaaagac acagatgacg attggcagaa aaaagatcca
    27301 ttgatccgct tccgtaaata tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa
    27361 caagtcatcg aagcaactaa agaagaaatc aaagcagcta ttgctgaagc cgacaaagta
    27421 ccaaaacaaa aggtttcaga tttcttgaaa aacatgttcg aagtatctcc acaatcaatc
    27481 aaagaacaaa ttgcaattta tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa
    27541 tgatccaagc aatcacagat gccttagcac ttgaactaga aaatgacgag aatgtcgttg
    27601 tttttggtga agatgttggg aaaaacggag gagttttccg ggcaactgaa ggattgcagg
    27661 aaaagttcgg agaagaccga gtattcgata ctcctttagc tgaatctgga atcgcaggat
    27721 tgagtttcgg tcttgcgtta gaaggatttc gtccagttcc tgaaatccaa ttctttggtt
    27781 ttattttcga agcaatggac gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga
    27841 gcggcactag aaatctaccc atcacaattc gttcgccatt tggtggcggt gttcatacac
    27901 cagaattgca ttcggataat ttagaaggtt tgatcgctca gtctcctggt attcgagtag
    27961 tgattccttc caacccttat gatgcaaaag gactattgat ttcttctatt agaagtaacg
    28021 atccggttgt tttcctagaa cacatgaaac tatatcgctc attccgagaa gaagtgccgg
    28081 atgaagctta cgaagttcct ttggataaag cagcagttac tcgtgaggga acagatgtat
    28141 ctattatcac atacggagca atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa
    28201 aagaaaatat atctgttgaa atcattgatc ttcgtacagt tgcgccatta gatgtagaaa
    28261 cgatcattca atccgtggaa aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac
    28321 aagcgggcgt tgccgcgcaa gtagtttctg agatctcaga acgcgctatc ctttcattag
    28381 aagccccaat cggacgagtt tctgcacctg atacagtctt cccatttgga caagcagaaa
    28441 atgtctggtt gccaaatgca aaagatatcg aagacaaagt aaaagaaatc gcggaatttt
    28501 aagaaaagaa gggacgaaac aaagtcatgg cttatcaatt taaatt
//
LOCUS       DACEOE010000017        28455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEOE010000017 DACEOE010000000
VERSION     DACEOE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.017"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            79..1125
                     /locus_tag="IW774_000652"
     CDS             79..1125
                     /locus_tag="IW774_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1153026.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IW774_000653"
     CDS             1474..2106
                     /locus_tag="IW774_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1153027.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IW774_000654"
     CDS             2694..4133
                     /locus_tag="IW774_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1153028.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IW774_000655"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IW774_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IW774_000656"
     CDS             4895..5110
                     /locus_tag="IW774_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153029.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IW774_000657"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IW774_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IW774_000658"
     CDS             complement(5770..6681)
                     /locus_tag="IW774_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1153030.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IW774_000659"
     CDS             complement(6700..7704)
                     /locus_tag="IW774_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1153031.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IW774_000660"
     CDS             complement(7701..9824)
                     /locus_tag="IW774_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1153032.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="IW774_000661"
     CDS             complement(9829..12276)
                     /locus_tag="IW774_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1153033.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="IW774_000662"
     CDS             complement(12263..12652)
                     /locus_tag="IW774_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1153034.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="IW774_000663"
     CDS             complement(12712..13215)
                     /locus_tag="IW774_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1153035.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13452)
                     /locus_tag="IW774_000664"
     CDS             complement(13228..13452)
                     /locus_tag="IW774_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153036.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="IW774_000665"
     CDS             complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="IW774_000665"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1153037.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16212..16331)
                     /locus_tag="IW774_000666"
     CDS             complement(16212..16331)
                     /locus_tag="IW774_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1153038.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(16346..17530)
                     /locus_tag="IW774_000667"
     CDS             complement(16346..17530)
                     /locus_tag="IW774_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1153039.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(17789..18079)
                     /locus_tag="IW774_000668"
     CDS             complement(17789..18079)
                     /locus_tag="IW774_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153040.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="IW774_000669"
                     /pseudo
     CDS             complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="IW774_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19444..20118)
                     /locus_tag="IW774_000670"
     CDS             complement(19444..20118)
                     /locus_tag="IW774_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1153041.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20196..21536)
                     /locus_tag="IW774_000671"
     CDS             complement(20196..21536)
                     /locus_tag="IW774_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1153042.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(21619..21954)
                     /locus_tag="IW774_000672"
     CDS             complement(21619..21954)
                     /locus_tag="IW774_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1153043.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(21964..22338)
                     /locus_tag="IW774_000673"
     CDS             complement(21964..22338)
                     /locus_tag="IW774_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1153044.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22351..22665)
                     /locus_tag="IW774_000674"
     CDS             complement(22351..22665)
                     /locus_tag="IW774_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1153045.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="IW774_000675"
     CDS             complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="IW774_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1153046.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(26419..27585)
                     /locus_tag="IW774_000676"
     CDS             complement(26419..27585)
                     /locus_tag="IW774_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1153047.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            27610..27936
                     /locus_tag="IW774_000677"
     CDS             27610..27936
                     /locus_tag="IW774_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153048.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            28089..>28455
                     /locus_tag="IW774_000678"
     CDS             28089..>28455
                     /locus_tag="IW774_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1153049.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNF"
BASE COUNT         8331 a         5791 c         4944 g         9389 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaacccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    13441 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    13501 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    13561 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    13621 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    13681 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    13741 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    13801 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    13861 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    13921 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    13981 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    14041 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    14101 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    14161 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    14221 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    14281 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    14341 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    14401 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt tcttgtgata
    14461 cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct ttttcagcac
    14521 tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata tacatttcct
    14581 ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta ccgcttttat
    14641 tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt tctacccaat
    14701 ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc ccttgcggag
    14761 ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct tttaatatcc
    14821 tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc tgttttatca
    14881 gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc acataatgta
    14941 gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact gacctattag
    15001 gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct aatacttgtt
    15061 ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt ctggttttac
    15121 catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca tgtggactgc
    15181 ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg ccaatatctt
    15241 taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg atatttcgat
    15301 atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg aaaatatctc
    15361 cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa tattcagcgt
    15421 gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg cttcactcct
    15481 ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa acaacataat
    15541 acttttctaa tatatacacg acttgagggc attcctccat tctcattaca agaatttctt
    15601 aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg tcagtttatt
    15661 ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata catatatatc
    15721 cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta tatggcattt
    15781 cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt tgcatttcta
    15841 caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata gaacattcta
    15901 accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc tggatagttt
    15961 tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct ttaccgagct
    16021 tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag gcgtttcaga
    16081 gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca aactttttcg
    16141 ttcttttatt agtgccttag cttttcacta aggaacgagt cgcacataaa ttttgtccga
    16201 aattcatctt gtcactttcc tttcttttcc attgctcgat ccgctgctgc acttgcattc
    16261 atcaagcaca tcacagtaac accaagcata ctgccaccaa ataccaaaaa aatatctttt
    16321 acaattccca tgacgattcc tcttttcata taatcaaatt ttcaattgcc atactttgtt
    16381 gctccaaaat ctttttatgt cggtctgata agcgtgcatt ctgtaccata tcctggatat
    16441 aggttgtccg attcaatcga tccaactcct tagccatttt ccacgtaggg gcgacttgtc
    16501 ggcctagcca attcaaggtt cgttcaatgg tatacggttt tggctcggaa gttaattgaa
    16561 tctctttccg attccgtcca ataaaacgcc cccattgttc attcaagggc cagttggttc
    16621 gccttttacc ttctacttta tccgcaaatc gcaagtaacg attgataatg gaaaacgtgg
    16681 tgctctcaat gctacgacct tttaacaaat cttgaatcgc atgaaaagca cggtcatttt
    16741 tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc taatggaatc cctaacttga
    16801 tatattgttc atagtctttt tcatacaaac agaaataaac ctcactcttg agactgccga
    16861 tatataaggt attgcccatg ccgtcctttt catcagcctt taaaagttcc ccggaacgat
    16921 aacttttaaa ggtacgaaac aaggagatac attcttcctt ctgacatttt ttcgttagct
    16981 caggtatatc caatagcccc actcgatcat tgattgccaa gtccaagcgt ttcatcacgc
    17041 caccggcctt tagacaatcc tcgaaaaagt cgtaccagct acgcttttga gcgagtaaaa
    17101 aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa aagcacacct ttatcttctt
    17161 catgagacaa catcaccaca atatcaccca tgacatactg ttcttgatag gagtaaaagg
    17221 cataatcttc atggagcata tactcaaatt tcaaatggag aatttcttga ataatttttc
    17281 gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa cagtaacgtc aaaggcaaat
    17341 cgggattaaa gctttccagt tggtcaaaaa ttttcctcat ggtttttata gatgcggtta
    17401 cgccaccatt ttctatctga tttaagtacg gccgactaag tccacaagcc gtagccaatt
    17461 tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa tagccgataa tctaaattcc
    17521 gttgtgccaa gttctcacct ccaaattcaa ggtgttaact ttttcaaatg agttaacacc
    17581 tatacaaatt acttactatc tttgatacaa caagcattct taatggttgc ttacttattt
    17641 tttgtgtttt tgtacccccc tgttagatac gggggtgaga catcatggtc ggctaacgcc
    17701 gtccatgaac aggcacagca ccacggagga tttctgcttc gcgaatcctc cgcggtcagc
    17761 gcctaacagt cagttgtgtt ggtttaggtt actcatctgt aataccattt acttccaact
    17821 ctgctttcat ttctaacaag tattgacgaa tatctggttc ttttgcttgg tccgcaatcc
    17881 gctcaaattc tgcaattact tgatttattt caattcgagc atccgtctcc tcattcatta
    17941 aatatggatc gccagcgact ttgacaccat tcttgcgaat atcaaatttt aaccgtcgca
    18001 attgttcttc taattctggg tctgactgaa tctttccaat cacccaataa atttctccaa
    18061 tcaactcatc ataaaccatg tacatccaac tcttttaatc ttctggcaat atcaaaaatg
    18121 accggaactg ttacagagtt tccggcttgc ttatacaact gactatctga attgacttga
    18181 gaatctcgat caaaagccca atcgggaaag ccctgaagtc gccaacactc tctaggtgtt
    18241 agcttccgaa tttgatactg atccgtgacg atcgcttgtt ggtctcctgt tagtaaggta
    18301 tgaacactct gttttcccag acgacctcgg cgacttgtgg aattaggaaa agccagattc
    18361 acactgtctt ctggcttcac ctcttgatat tctgtttcat tttttcgaat catgattttt
    18421 gtaccacctt gtttcgttga caaggtgggg gcggttccag taacttcata aactcgatta
    18481 ccttgttcaa actttcccgg taatagtcct gctacagaga ttccatccct tgctggcagt
    18541 gcgattttga ttccttcatc acctgttaag gttggcgcta cgccatctga accatagact
    18601 tgtccaccca ttcctcgatt ggaaggattg atatttacca aatttctgat gttttgatca
    18661 gatgttgaag acggtccaat gataggaaat acttgttcgg tacatgcgtc tcgagaatgt
    18721 ccgataagga atatccgctc ccggctttgg ggaatgtagg cctttgagtt aagcacctac
    18781 cactccacgt cataccccaa tccatccagc gttcggagga tcgtctcgaa cgtagcccct
    18841 ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata aaagcttagg tcggagaaga
    18901 gtggtgaacc gagcgatttc aaagaataaa gtacctcgag tatcggaaaa tctttgccgc
    18961 tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc aaaggatgtc cactggtccg
    19021 agggattgaa taaactcatc tgatacactt gtgatgtcat gcatttccac ctccttactt
    19081 gtatcatgaa ttgctttata actacgtcta gcaaatttat caatttcaca aaaaccgata
    19141 caatgatggc tcgcctgttc catacctaaa cgaaagccac caataccggc gaataaatct
    19201 aaaaatctca tacattacct cattcattgc aaagaaaaaa gccaagaaca ttttgctctt
    19261 ggctcataca ttattaattt gtcctactat tttttattta atccaactaa tttccgttgt
    19321 ttttcattgg cctgatcggc tacatattta atcaccgttt tgaactcttt tgcagtagca
    19381 ttttccgata aatccgcaat gctcttattg tatttcttta caattttcgt tacttgttct
    19441 ttctcagtca tgtaattgaa cctccaaatc tttcctacta taccctagtg gtaaaaataa
    19501 ttctagatta tccgtagcat aatgaacttc ggaaatatta ttcgtataat cttttattat
    19561 gatatgaagc aatacatctg gatgtttact ctttactttt gcaatgttct ctcttgattt
    19621 gaaaaaagcg ttaataaatg cctttttcgg cacatgacgt ccctctgcaa tttcacgttc
    19681 ttgtgtaaat ttccaagcga ttttagggtc ttgataaaca tagaaaatcg tcactcgaaa
    19741 atgttttttc aacgcccgcg agatattttt ttcagcactt aatattgcaa aagttgcatc
    19801 cagaataaaa gaatagccat gatctaagac atatctaaat gcctgctcta ccaaccaaga
    19861 agatgctttt tgaaagagac tagagttttt tccattataa tcaggaaact gggtgcgaaa
    19921 gtaatccgca tcaataatca catggttatc ataattctca gctaatccaa tggcaacctc
    19981 tgattttcca gccccaggac tccctgccat aaaaactgcg tctcgtccaa cttcggggga
    20041 tttccctttg ataatataag aaataaactt atccttattt gcttttgcat actctaaata
    20101 agaatcatta ctttccatat taattatccc ctttcttcct actatataaa agtattatac
    20161 gttacttgaa gtataaagtc agtattgaag atcactcaat cattcatctt ttctaattta
    20221 ctaattgaat ctaagaaatc ataccctttt ggtacaagtg gcgtataaaa ctcactaatc
    20281 acactgccac ctgtatccac ataacctcga cctttgatgt gcttcataaa gaagtttttt
    20341 tcgacctccc caaacatcat ggaatatccg agttcactca tacgacccaa agccacgcga
    20401 aagttgaatt gatcgcggat tccgtctcct aaatatttcg catccggcct ttggcaagcc
    20461 aaaatcagaa agaaaccaga ttgacgaccc aacatcacga tttgtttgag cttattcaaa
    20521 atcaccgcac tttctttcgt cgttaacatc tccatataag ccacgtattc atcaaagatt
    20581 aaaaagttgg gtggaagtcc aagataggca taattctctc ctgttttgta gttagacatt
    20641 tctttcattg ccttgctacg agccatcata cgttcataga aatcttccac acaagcagaa
    20701 atttcttcct tttgtgaata aacatgaggc atcaccgtac ctaaatcagc taaatctgca
    20761 tttttgggat cgaggataaa caattctgca tctgacttca atagtgcttc gataatggtg
    20821 aggaggaaat aggtcttccc tccacccgtg ccaccagcga ttaacatatg gggtaaagaa
    20881 tcataagccc atacttgatt tttcatcaat cgcagagtcc cattttctgc cactacttcg
    20941 tctatcccaa ttcgattggc gatcatatca tacagtaaag tatactccac ataggagtct
    21001 ttgagttcct tttctactaa ctcacaatac agtccgcttt ctaacttctt ttccaatttt
    21061 aaaagctgat cttggtattt cccaagtgaa atttgaacgc gaatagacag taatccatct
    21121 ttcattcgat aatagatttt ggggaagtaa ctgatagtct ctctggttcg gctactattc
    21181 aaatctttga aaaagccttc actttgagtt tgtttcactt cataccaatg gttctctaga
    21241 atcatccgag ctagcttttg tcgatgccac aagcttcgcc aatgatcaag aaaaaagtga
    21301 atataaagca atatcatagc tcctgaaaaa gcgagacttc caagcagaca tactaaatct
    21361 tttatagacc aaacgatttc aatagtagaa agtgatagct tagaaatatc aaattgtcta
    21421 aactcttgaa aataaaagac gctcatccaa ccgatgaaca gagcgagtag ccagccaaga
    21481 acaaagtgat aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt gtacattttg
    21541 atggtttcca cctccaatcg aaaaagccca ataggatttc attaggcaat ttcaaaaagt
    21601 tatgctagtc atttactgct acgaggaggg gagttgtttc gtttcaacca attgatttaa
    21661 aaagccttca atagcatccg gcatctccac accaatttct tccaaaatgg attctgcctg
    21721 ccgacataag ttttcattta cttcgacaac tataaagaaa gactcttctt ctcctaacca
    21781 gtctagtaca tcagagtctt cttcactgtc gctattttct ccaagtaaac cttgatgtat
    21841 aatcagaaac attttttcat cagaggttaa tgctttaacg agttgttcct cagtcagctc
    21901 gccttttcca tattgatcga gacaatctaa aatatgttca acattaattt ccataatctt
    21961 cacctatttc tttctttcat tttggttcgt aggatttcct tgattcggct gccgcttcaa
    22021 gactaaatca tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa aaacataatt
    22081 ggcaacggta tccacgattg gattgaccaa ctctacctct gcatcataag caaactcgcg
    22141 taagggtaca ttagctggga tactaacttg aatcattcgc ccttgtgcag aggattttaa
    22201 attataggtt cgttctttta cctcattacc aattgttcca tcctcatttt gcttccgtac
    22261 ttctcggcgt aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat ctacgacaat
    22321 accttctgtt aatctcatct tcttttcctc ctatgctttc actaaatcat caacatacaa
    22381 cacgtaatca gtaaacccac gattctcaat ctcatagcct ttggcaacca aatggggatt
    22441 cacggctttt agcggttggt cttgatcaaa atccttttca ccggcttccg cagctactac
    22501 cacctcaata tcatcggccc gttgcttact ggaatacaaa tgataactgc gaccaatcac
    22561 cgtagtattc cgtccatatc cttctgttat aatttctcct tcaccggcat aacttaactt
    22621 tccaaaggtt tcagccatat ctggcacgac aaatttcaat tccatcttct tttcctcttt
    22681 tcattgttag ttttggatac aaaaatagcg cgactacttt tttaggaggc ggtcgcgcac
    22741 gttggttctt aggatttact tgtatcatct catcacctta gttccggcga ttccagaaat
    22801 aatagcccgc cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca ttggaatttt
    22861 tctccccggt ttgagggaag gtcttcactg tgggactaga ttcttgagag ccacttggaa
    22921 cagttggtgt ttccggttgt ttcggcgtac ttagtatggt tggaacttct tttggtgtta
    22981 aggtttgaga tttttctttc aaatcttcat tgtgttttcc attgatattc ccatctttat
    23041 cgtaattgat ttccgcaaaa gtaaacttgg ttccttctgg atattttccg gtatctactt
    23101 ttttcgcaag cacagttttg gtgaattctt tatcatttac ttcataatca atcttgccag
    23161 atttccaaat ttctttgttt gtaccatccg gtaataaagc atacagaatt gtttcgaaag
    23221 cttcttttga gccatccagt acatcatgag taatcgacac atcatcaaac atatccacca
    23281 catcaccatg agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt tggatagaaa
    23341 catggtgttc cactgtacaa ctgacagttt gggcttggtt attcaagcta acatccttcg
    23401 ctactggctt atcgcctgct tcaaaagctt ccttattttc atacacatag ttgaataaga
    23461 caatagtttt atttaaaact tgttcggcgg ttaatttatg ctgaattttc caagttcctg
    23521 ttttctcttt gttcgccaac gttgcgcttg tttctgcaat caccactggt ttggcttttt
    23581 catctttttc ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct accacatacc
    23641 agtcttcttt gatttcatgt agattgtaac tcaatttatc aactagctcg gtagaatcca
    23701 gagaagttaa ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa ctatcttctt
    23761 cctcaggcaa atcatagagc attaaacggt tcaaactaac agaaaatgct ttgttcgtca
    23821 gcttctcgta aggaacggtc accgttttaa ttggttgttt ttggtcttgt tcggtaatgg
    23881 taaagacata ctcactcttc acaaagtctt ctttgttttc cttaaaagta gaacggattt
    23941 ctaatggcgt aatcttttga aaaccttctg gggcttctac ttcttcaagt aaataatccc
    24001 cataaggaag attttcaaac ttgccataac catcaaaccc taattgctcg ttataagctg
    24061 tggtcgcttc atcttcggca cctgtgattt cattggtccc ttccaatggc gacactttaa
    24121 aggtcagatc attgaagcca gtttcggtag tgcccgcagc tgatccagca aatttaaaga
    24181 aatcaaagcc aaagcgaatc acttgttctt ttgccgtaac atttcgggta atcacggcat
    24241 ttttttcgct atcatccacc tttttgatgg atacaggata ctttgtttca tcaaggctat
    24301 atccttcggg cgcttttgtt tcttgccaat aatactcgtt gatagctaaa tgtttaacgg
    24361 caacttggtt cttttcgtct aaagtcaaag tgaccgtttc atcagaacct ttcgttccct
    24421 tcgctaactc tggtttaaaa gcctcactcc atttaacagc ttttccatcc ttagcagtaa
    24481 acaaggtgta ttcagcaccc tcaaagactg ccttaccttg tgctttatcc ccagtatctt
    24541 tgtcttcttt cgtcaaagtg gtttcgccag tgacttcttg attttgccct tttacgttac
    24601 tggtaacaag ggctacggtt tgattggcat acttcaattc gacttttaca ggtttgaacg
    24661 tattcacgaa cccatgacta gctttagttt cggtcacata ataggttccc aattccaacg
    24721 cattagcaat ctcttttggt gtttccgcat gaccgttttc atccgtggtc atttcttgaa
    24781 cgatttcacc ggttggactg tctttgcgaa tggcaaacgt attaccagct aaggaataat
    24841 tgtcattcca aagatcactc cctgtttcaa ctccgctttt atccagaatg atttgccctt
    24901 tttcccgtgc gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg gtggtagtca
    24961 tgggggtggt atcaatcgta taaggcgccg gtactgattt ttcggtaatg gttacttttg
    25021 taccatgagg aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc acgtcttttg
    25081 caggtaaagt ttttccaaag tctaaatgga aaaccgttcc tggtacaaca tcacccgatt
    25141 ctttatcgac ttttttgatc tttaaagaac cctccgtttc cacatcaatt ttgattgtgt
    25201 aggtattagg tttatcaatc gccccggcca ttaaagtctg ttggccagcc tttttatagg
    25261 cgacaggtgt tccggtacca gcagatttct taagcgtcag cacaccactt tcttttgaat
    25321 tggcatttgg ggtgattatc aactgattac cattcacacg ataatcaatg tttgcggcat
    25381 tttccacaac ctcatcgaac tctgacaaat tcagattatt cgtatccgtc acagtagtcg
    25441 attgccctaa tactgtttta gccgtactat tatggaaggt tggtttcttc tgataatcgt
    25501 caatggcttg atttattttt gcctcaatgg ctgcgatatt tacggcacta ccatctgatc
    25561 gttttattga gtgaagggta tacccattaa cttcttgcca gatcatcttc tgtgccacaa
    25621 tatgagtatc tgcatctgta cctacttttt tccataaaac ggaaactaat ttggctttat
    25681 cggacatatc tggtaacggg tttttctcat agccaggagt gaactcattg taaatcggga
    25741 ctcctggttc aatacagaaa atatcttgtt taaagccgtt gtacactgca taatgcggaa
    25801 cagtgacttc tgacattgga cgaccatcac taaaagttcc ttcaacagca tattgatgtg
    25861 ctaaatctag ttctacggtt accgtttgtg gatgggtaat ttcatcagca aaagtaatcg
    25921 taggagcaat tgctccaaca attgcttgaa cagccaaact caaaatagtt aatacacata
    25981 atttcttttt ttgtttttta tacatgaaat gttctccttt caaattaagc acaaaaaaat
    26041 aagatgctcg cttgaacatc tttgtttata aagtcttttt aaaattttta ttgactgaat
    26101 agaatggatt tgagaatgaa tgagcctaaa aatttaatct aaaatacgaa atatactgcc
    26161 tttaaattag aacacatttt agaacacaaa catttcaaat ttaatcaaat ataataaaat
    26221 atatttacgg gaaaaccttt ataaatcaag gtttttcaaa cttattcaat gatacgaaga
    26281 aaccaggtct taagaaagca cgtaaagctt cacagttttc aaaacgttaa tcgttgttat
    26341 accaacgatt tcagacactt tctcattgcg agaaggtgtc tttttttcgt tctggtaatg
    26401 agaaaggtaa tgacaatatt ataaaccagt gtaagcaatg aatttttcta acgcatttcg
    26461 ttgagctttt tcagtaacat gagaataagt gttaagggta gtttcagcat ttttatgacc
    26521 caatcgatct tgtgtttctt tgatacttgc tccaccttct agtagaagag tagcgtgagt
    26581 gtgccggaat ccatgaatag taacgtgctt aaatttatat ttcttctcta aacgtttcaa
    26641 tatattattt gaatagtccg catgcaaagg ttgattaatg tcaccgtctg catttacata
    26701 ggtaaagata agttgatttt ttgtatttgt atttatgcca aactgcaaaa gctctcgagc
    26761 ttgttctttt ttccagattt ttaacatttc aatagtttgt gcatcaattg gtaatattcg
    26821 ttcggaagat atattttttg tatttttttg aatcctgaca gtttttcttc ttgcattctt
    26881 ctcttcattt cgaatagttg catagctctt tctaacatac attagctgtt cttcaaaata
    26941 aatgtcagac caatgaagag cagctagctc tcctttacgt ataccagtag taatcaaaag
    27001 ctggaagatt aaaatatgtt ttaaaggctc ttctttattg agaatatccc aaaaagcttt
    27061 tagttcttct aatgaaaagt aattttcatt ttctttcttt tcccgctttg gtagatttgt
    27121 atttgccata ggatttcggt gtaatagctc tgcaaaaatg gcatactcaa atatttgaga
    27181 gctgtaagac ttaagctgtt tgatattctt gaattttaaa gcccattcat tgactgcatt
    27241 ctgacagtga acaggagtaa tcttagaaat ggacttattc ccgaattttg gtagtatatg
    27301 caagcggaaa aggtcttttg tacgttgata tgtcgtttct tgtacacgat tcctataaga
    27361 aggcaaccat tcttcataca ctgttataaa caagtcatta gaaggcttat gatagccggt
    27421 atcttcaatc tcgacctttt ttcttgcttc gtgtaacttg gcttcttttt cagttttaaa
    27481 cccttttttt acatagtatt cttgctttcc attttcatct ttacgaacat acaagcggac
    27541 actatatcgt gcaccgcttt ttgttgtgta cttttgtatt gacataacta atctctccaa
    27601 tctgataact tggcgagttg gctgattgaa gaaatagaaa atgagaggag aaaaataatg
    27661 gatgcaggaa ttacagtaat gttagataaa caacaaacga atcaattgaa aaattatgtt
    27721 tttgaaatga caaaagaggc tattgatcaa gcaagaattg ataccggatt agaaagacca
    27781 tttctaaaag gaaaagagat ggctaaatat ttgaatgtat catatacaac ttttttgaaa
    27841 tttaaaagaa tgggattgcc agttatttta ttagaaaaaa tggagttgtt ttcaaaagaa
    27901 gaatgcaaaa aatggatatt gagtcatcaa atttaaaaaa cacctcgttt tttaaatttg
    27961 atgaagtaga aaaaattaaa aagagtgttc ccgtcagttt gcagatcggg ggaacactca
    28021 ataataaagt tctgagaaaa ctttatttga atctattata gcaaataaaa tcagaagcag
    28081 gtgtgacaat gaatgaaaaa gaagttgaaa gcttagttga aaagcaaggc tattctaatt
    28141 ctgatatata tgcagaatta tactatcaat atcctatttt tttaaacgta aatgaaaaat
    28201 ataagaaaat gaaactaagt tctaagttag tttatatgtt actaaaagat agaaatagac
    28261 tttcattgaa aaataacttg tatgatgaga atcaaaaaat ttttttgata tttgaaatca
    28321 aaaatctcgt agaaatttcg ggaatgtcaa aaaatacaat cttatcagca ctagatgaac
    28381 ttcaaaaagt taaacttctt gtgaaaagaa aaaatggctt caataaaagt aaaatgcaaa
    28441 tgttcccaaa ttttt
//
LOCUS       DACEOE010000018        26880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEOE010000018 DACEOE010000000
VERSION     DACEOE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.019"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            513..1220
                     /locus_tag="IW774_000679"
     CDS             513..1220
                     /locus_tag="IW774_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1153050.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1279..1923)
                     /locus_tag="IW774_000680"
     CDS             complement(1279..1923)
                     /locus_tag="IW774_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1153051.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            2049..2336
                     /locus_tag="IW774_000681"
     CDS             2049..2336
                     /locus_tag="IW774_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153052.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2444..2764
                     /locus_tag="IW774_000682"
     CDS             2444..2764
                     /locus_tag="IW774_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1153053.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2845..4158
                     /locus_tag="IW774_000683"
     CDS             2845..4158
                     /locus_tag="IW774_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1153054.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            4155..4874
                     /locus_tag="IW774_000684"
     CDS             4155..4874
                     /locus_tag="IW774_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1153055.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            5002..5553
                     /gene="raiA"
                     /locus_tag="IW774_000685"
     CDS             5002..5553
                     /gene="raiA"
                     /locus_tag="IW774_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1153056.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5775..8309
                     /gene="secA"
                     /locus_tag="IW774_000686"
     CDS             5775..8309
                     /gene="secA"
                     /locus_tag="IW774_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1153057.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8435..9536
                     /gene="prfB"
                     /locus_tag="IW774_000687"
     CDS             join(8435..8497,8499..9536)
                     /gene="prfB"
                     /locus_tag="IW774_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1153058.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9641..10327
                     /gene="ftsE"
                     /locus_tag="IW774_000688"
     CDS             9641..10327
                     /gene="ftsE"
                     /locus_tag="IW774_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1153059.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10311..11204
                     /locus_tag="IW774_000689"
     CDS             10311..11204
                     /locus_tag="IW774_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1153060.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11356..12804
                     /gene="cls"
                     /locus_tag="IW774_000690"
     CDS             11356..12804
                     /gene="cls"
                     /locus_tag="IW774_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1153061.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNIGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13141..13998
                     /locus_tag="IW774_000691"
     CDS             13141..13998
                     /locus_tag="IW774_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1153062.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14113..15033
                     /gene="pstC"
                     /locus_tag="IW774_000692"
     CDS             14113..15033
                     /gene="pstC"
                     /locus_tag="IW774_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1153063.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15033..15917
                     /gene="pstA"
                     /locus_tag="IW774_000693"
     CDS             15033..15917
                     /gene="pstA"
                     /locus_tag="IW774_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1153064.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15932..16738
                     /gene="pstB"
                     /locus_tag="IW774_000694"
     CDS             15932..16738
                     /gene="pstB"
                     /locus_tag="IW774_000694"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153065.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16754..17512
                     /locus_tag="IW774_000695"
     CDS             16754..17512
                     /locus_tag="IW774_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153066.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17525..18202
                     /gene="phoU"
                     /locus_tag="IW774_000696"
     CDS             17525..18202
                     /gene="phoU"
                     /locus_tag="IW774_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1153067.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18439..19470
                     /locus_tag="IW774_000697"
     CDS             18439..19470
                     /locus_tag="IW774_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1153068.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19467..20234
                     /locus_tag="IW774_000698"
     CDS             19467..20234
                     /locus_tag="IW774_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1153069.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20334..21902
                     /gene="liaX"
                     /locus_tag="IW774_000699"
     CDS             20334..21902
                     /gene="liaX"
                     /locus_tag="IW774_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1153070.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSVIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22202..22519
                     /locus_tag="IW774_000700"
     CDS             22202..22519
                     /locus_tag="IW774_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1153071.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22519..22869
                     /locus_tag="IW774_000701"
     CDS             22519..22869
                     /locus_tag="IW774_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1153072.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23036..23974
                     /locus_tag="IW774_000702"
     CDS             23036..23974
                     /locus_tag="IW774_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1153073.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23989..24819
                     /locus_tag="IW774_000703"
     CDS             23989..24819
                     /locus_tag="IW774_000703"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1153074.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24863..25888
                     /locus_tag="IW774_000704"
     CDS             24863..25888
                     /locus_tag="IW774_000704"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1153075.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25903..26841
                     /gene="galU"
                     /locus_tag="IW774_000705"
     CDS             25903..26841
                     /gene="galU"
                     /locus_tag="IW774_000705"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1153076.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
BASE COUNT         8831 a         4647 c         5807 g         7595 t
ORIGIN      
        1 taccacaatc gtaagctctc gttagttcga gagctctttt ttttgctttg ctaccttgtt
       61 ccttgaagat tctgctgatt taagatagag ttaagttaac tacaagaatt ggagggatcc
      121 acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa gttatatgat ttggtcaaat
      181 cagaaaaata caatattgaa gtgaatattc cgaaaaaagg cggaaaagcg gtccgggtaa
      241 aatcaaaacg cccaacgaat catacaaaaa aatggatggc taaaaaccgc taataataac
      301 aaaagatcca taagtcggca ttttcatcga ttttagggtc ttttgttatt ttctaggttg
      361 gacatatcta aaaaattgtt tctgacaata ggagtaggaa gaacgatgtg tgtctaaatc
      421 ctctgttgat ggatagaaac ctttatcttt ttcagttgtg aatcaggtat ataccagtta
      481 taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa tcaattacct gtatatatac
      541 agattcatga ccaattaaaa aatgaaatcg aacaaggtat atggaaagtc ggagaccgtc
      601 ttccttcaga aagagaatta gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag
      661 caattcaaaa cttggcagat gaaggaatct tagaaagaaa gatcggttcg ggtacatatg
      721 ttgcacgaga aaaagtacaa gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg
      781 aatcacaaaa tcgagtaccg tctagtcgga caatctctta tttcttgact tcgccaagct
      841 caagtgaaat ggaaaaacta aacttgagta aagatgacac gatcttgcga atggaacgta
      901 ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc cagtgtgcca caaaagctga
      961 tcgatggcta cagcaaagca gagattactg aatcgttgta tcgcacgcta gaagaaaaag
     1021 gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa
     1081 ttgctgacta tttagaggta aaacgaggag atgcagttct tcgtttgcgc cagatttctt
     1141 attttgaaga cggtacgccg tttgagtatg tgcgtacgca atacgtagga aatcgttttg
     1201 agttttattt ggaaaaataa aaaacaggtg atccctgatt ccgtattaga aataaaagag
     1261 aatcagaatc acctgttttt atatatgatc agttaccagt tgctcgtgat aagaaacagg
     1321 accctcttca aattggactt gtccgttcag cagctcaatg atggattctt taaagtggtc
     1381 agcacttttt tcatctacag cacagatgac gctaacttga tcagtatatt ggatttcttt
     1441 tataaaaaaa tgttggagtt caagataatt ctgtaatttt cctagttgcg gatacgtgat
     1501 catcacagag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg
     1561 agagaccgca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa
     1621 atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga cttccaacat
     1681 cggtatgccc gccgttccac ttggttctcc atcatcgctg cttcgctgga tttcattttt
     1741 ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt tttttatttc
     1801 ttgaatgaaa ctttttgctt cgtcctctga ttctattcgt ctaagagaac agataaagcg
     1861 tgattttttg atctcgatct cactttgtcc attttctttt attgtacgat agcgctcgat
     1921 catcttgtca cctctttttt cattttcttt attcagtata aataaagttc taggttgttt
     1981 caacactgta acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg
     2041 atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca
     2101 atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta
     2161 acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc
     2221 ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg
     2281 acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa
     2341 accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt aacagacgcg
     2401 aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta aaaaaacaat
     2461 catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc
     2521 tgctgaagca aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa
     2581 caatcttgca aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa
     2641 agctgatttt gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc
     2701 tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga
     2761 ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc
     2821 gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga
     2881 gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca
     2941 gtggaacgtt tgagcaactg gattctttgt catcgctgtg gaatgagaac gtccgtcatt
     3001 gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt agggcgatgc
     3061 gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc
     3121 tttttttgga caggacagct tactgaaaaa caaacggaaa tctcaaagag aatactttgc
     3181 cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg
     3241 ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg
     3301 cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa
     3361 atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt
     3421 acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc
     3481 gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa
     3541 ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact aaaggagatc
     3601 aagcagacgt ttgacatcca caaacttgct ttacgctttc atcggcgttt gcttcctgaa
     3661 cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa gaagataaaa
     3721 tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt attttgtcca
     3781 agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc gagatatgag
     3841 atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa aatgcgagaa
     3901 gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac atttgaaaga
     3961 atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc tttggtacaa
     4021 atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata ttttttctat
     4081 gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat gaatgaagaa
     4141 gggaagaaga tactgtgagg tgcagttgct gcagtaaaga tatcgtccaa aatttaacat
     4201 tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca gtttgtcgcg
     4261 cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca atccatttca
     4321 atctagagag tacgaaaaag aaagtaaatt ctatccccgt ttactgcagt gactgtcaag
     4381 aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt gtgtacgatg
     4441 aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga ctagcaggca
     4501 cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc tattgtccaa
     4561 tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc gagatgttga
     4621 aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg ctggctcaag
     4681 caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt gcagtcacct
     4741 tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca acaggacaga
     4801 ctcttttcca cgcagcagat tgtttgcaga tcgcacagcc gaaaatcatc cgaactattt
     4861 ctttggcgag atgaattaga tgtggaaatt tgatagcgat ttaatttgca aataacctag
     4921 agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc ttgattgtgg
     4981 tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa tatcgaagta
     5041 actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg ctatttcagt
     5101 gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa
     5161 gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac ttctccagat
     5221 ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg caaattcaaa
     5281 acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcaaatgc tgctgcttta
     5341 ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg tacgaaacgc
     5401 ctttcattga aaccaatgga tagtgaagaa gcagtcttgc aaatgaacat gctgggacat
     5461 aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa
     5521 gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata acaacgaaaa
     5581 cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc ttttagatga
     5641 aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca attatctata
     5701 actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag aaggaaaagg
     5761 agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat aagaaagaac
     5821 taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa atggcagcat
     5881 taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac caaaaaggag
     5941 aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca gcaaaacgtg
     6001 tattaggttt atatccatac cacgtacaat tgatgggtgg aatcgtgctt cacgatggaa
     6061 atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg ccagtatatc
     6121 taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat ttagcaaccc
     6181 gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg gggttgaata
     6241 tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc acttatagta
     6301 cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac cgccatcaaa
     6361 tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc ttgattgatg
     6421 aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct ttatatacaa
     6481 gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc gatgtacaat
     6541 caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca ttcggtttgg
     6601 ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa gcattgagag
     6661 ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa gtactgattg
     6721 ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga cttcaccaag
     6781 caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg gcgacgatca
     6841 ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt acagctaaaa
     6901 cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt ccgacaaatc
     6961 gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa agcaagttcc
     7021 acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg ttagtcggaa
     7081 ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa cgtattcctc
     7141 atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg aatgctggtc
     7201 aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat atcaagttag
     7261 gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga catgaatctc
     7321 gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct ggtatgtcac
     7381 agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa cgaatcaaag
     7441 ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc aagatgctga
     7501 cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac acacgtaaaa
     7561 acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat aaacagcgac
     7621 aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg gtcaaacgga
     7681 caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaactgg aatttggaaa
     7741 gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact cttgcagata
     7801 tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca caagaagtat
     7861 ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt gaaaaagtcg
     7921 tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgacgcaatg gatcaattac
     7981 gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa tatcaaacag
     8041 aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc actcgaatct
     8101 ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa ggaacagctt
     8161 ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa aaacagccaa
     8221 tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg aaaaaatata
     8281 agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg agacaaattc
     8341 tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt agactagata
     8401 taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa ttcgaaatct
     8461 cctgacagaa atgcaggaaa aggtcacaag tttcaggagg tctctttgac ttagatcagc
     8521 tggaagaaga catcgcggaa gcagaagcaa aaatggctga acctggtttt tgggatgaca
     8581 gcgaagccgc acaaaaagtg atcagtgaaa ataacgctaa taaagaaacc tatgaccaat
     8641 tccattctct tgccgaagaa ctagaagaaa tcgaattgat ggtggagatg ctgcaagaag
     8701 aacctgatgc agcaatgcaa gaagaagcag aaacacgtat caatgcatta gatgaaaaaa
     8761 tgaagacgta tgaattatct atgctgttag acggacctta tgatcgaaac aatgcgatcg
     8821 tcgaattgca tccaggggct ggcggaacgg agtcccaaga ctggggcagc atgcttttac
     8881 gtatgtatac acgttgggca gagcagcatg gctatcaagt agaaacacta gattatcaag
     8941 caggtgatga agcagggatc aagagtgtga ccctgctgat taaaggacat aacgcttttg
     9001 gttatctaaa atcagaaaaa ggtgttcatc gccttgtacg gatttcgcct tttgactcag
     9061 cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt gatgaaacga
     9121 tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca agtggtgcag
     9181 gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg cctacaggaa
     9241 cagtagtagc tagtcaggca caacgctcac aattgaaaaa ccgtgagcaa gcgatgggga
     9301 tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa gcagctgcac
     9361 ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat gttttccatc
     9421 cctattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc caagcagtta
     9481 tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta caataagaac
     9541 ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac attgccatgc
     9601 tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa tgaaggatgt
     9661 aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcgattg tcatcgaaca
     9721 aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat tcatcaaatt
     9781 gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc atgatctaat
     9841 agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg tgttccaaga
     9901 ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta tgcaagttat
     9961 cggcagaaaa cctcgagata tcaaaaaacg agtaatggaa gtgctggacc ttgttggttt
    10021 gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc aacgtgtctc
    10081 tattgctcgg gcaatcgtga atacgcctaa agtacttatc gcggatgaac cgacaggaaa
    10141 tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca atgcccaagg
    10201 aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc ggcaccgagt
    10261 aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat atggatacga
    10321 tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa cgtaacggct
    10381 ggatgacagt tgcttcagtc agtgcggtaa cgatcacgtt gactttagtc ggtgtattca
    10441 tggcagttat catgaatgca actaaattag ctcaggatat agaaggaaac gttgatgtat
    10501 ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa aaagaattga
    10561 atgacttaga tcatgttaaa aaagttgaat tttcaagtaa agaacaggaa ttgaaaaaaa
    10621 tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac ccgttatatg
    10681 atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca gaagaagcag
    10741 cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat cggattttcc
    10801 aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg ctgttcgtag
    10861 cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag cgtgaaattc
    10921 aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt ttccttgaag
    10981 gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca tttggttatg
    11041 catggttttt ccaattgatc aatccatcat tattacgttc ccattattca ttgattcatc
    11101 cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg atcattggtt
    11161 cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac aatataaata
    11221 aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta ggagaataga
    11281 aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta agttggtaga
    11341 ggaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct tttgattatg
    11401 aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc tcaaacatgg
    11461 gcatggctgc tcgttttgat gttcattcca gttgtaggat ttattttata tattttttta
    11521 ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa aatcggtaag
    11581 aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc tcatccgcct
    11641 actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga aagtacgctc
    11701 tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt cgatgcgctg
    11761 attgaagata tcaagcaagc agaagaccat atccacttcc agtattatat ttatcgcagt
    11821 gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg aggggtaaaa
    11881 gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt ttttcagaat
    11941 ttgaaaaaag caggcggaga aatcgcattt ttctttcctc tatttgttcc gtatattaat
    12001 cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa aatcggatat
    12061 acaggtgggt tcaatattgg aaatgaatat ctaggattgg taaagaaatt tggttattgg
    12121 cgagacaatc atttgcgtat ttacggagaa gcggtgtata tcttgcaaaa ccgttttttg
    12181 atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt ttttccgtcc
    12241 attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga taccgaacat
    12301 gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga gatattgatc
    12361 caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt ggctcttctt
    12421 tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct cgtttactgg
    12481 gcaacgtatt ctttcgctgc agagctgatc gagtatggag caaggatcga aacatatgag
    12541 aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc agttggctca
    12601 gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact gatttatgat
    12661 gaacgaatgg cttcacgtgt aagaaaagca ttttttgaag actcgaaaat ttctacgcat
    12721 ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga aggattagca
    12781 agattgattt cgccgctcct ttaaaacaca aaaaggacat gagaaccatc aacagatgga
    12841 gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat ggagaaagcg
    12901 tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa gcaagaaata
    12961 agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata gaaactttta
    13021 atgagatagt tttttttaca attatgagga tttattttgt tatttaatga ttttgattcg
    13081 ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag gagatttcta
    13141 atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt atcaggctgt
    13201 tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc tttacagcct
    13261 ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt catcaatgta
    13321 caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt cgacatcgga
    13381 aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt agtcgatcat
    13441 cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt aaaagacatc
    13501 agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag tgaagtcggt
    13561 ggaaaagatc agccagtagt tatattgaac cgtgcttcag gaagcggaac ccgtagcacg
    13621 tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca agattcaagc
    13681 ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt tgctttttct
    13741 tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac agatgaaaat
    13801 gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag agatcatcca
    13861 aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca agataacatc
    13921 gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga ttggcaagga
    13981 aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac ttttacatgt
    14041 tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa cctgattaag
    14101 ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa acgttcaaaa
    14161 atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt gttggttgta
    14221 ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac tgataagatc
    14281 aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg agcaaatggg
    14341 aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac ctttttatct
    14401 gctattgttg ccacaccatt tgccattggt gcagcagtat tcatgacgga gatctctcct
    14461 aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat tccttctgtc
    14521 gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat ttttggcggg
    14581 acagggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt gccgactgtc
    14641 acaacgatga ctgttgatgc attaaaggca gtaccacggc actatcggga agcttcatta
    14701 gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc agccgtgccc
    14761 gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc cttagctatc
    14821 caaatggtca tcggtaatgc agcattgatg ccaacaggac ttactactcc tgcttctaca
    14881 ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt tgaaaataat
    14941 gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa tatcgtgatc
    15001 cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca gataaaattg
    15061 ctacaggtgt tttatatgta gtttcgggga tcatcgtatt gattttagca gctttactac
    15121 tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc gaaccttcca
    15181 aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt tatttgcttt
    15241 tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat ctttcagaat
    15301 atgctggaaa aaactggctg acagatgtga ttcgcacgtc gattgaaatc ttgagctcat
    15361 tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa ttccagtatg
    15421 gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct cttttgacta
    15481 gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca ggattggctt
    15541 taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg acaccaggga
    15601 ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct gcattgatct
    15661 atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca ttaagtgttt
    15721 caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt tggaagatca
    15781 atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct gccgtgttga
    15841 tcattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga ttataccgaa
    15901 aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga atgaaacaaa
    15961 tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc tacatgtctg
    16021 gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa ataagatcac
    16081 tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt tgaatcgaat
    16141 gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag gcgtagatat
    16201 taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg tttttcaacg
    16261 tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga aacagcacgg
    16321 cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac aagctgcctt
    16381 gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg gcgggcaaca
    16441 gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc tgctagacga
    16501 gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac ttgtaaactt
    16561 gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcgg ctcgtatcag
    16621 cgactatact gcctttttct atctaggaaa agtaatggaa tatgacaaaa ctaacaagat
    16681 atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt ttggttaaga
    16741 cggaggcctc aacatgacaa aagaaattat ttcatctaaa gatcttcatc tgtattatgg
    16801 aaagaaagaa gcactaaaag ggatcgatct aagtttcaat caaggggaaa tcactgcgat
    16861 gatcggtcct tctggttgcg ggaaatccac ttatctgcgt tctttgaacc ggatgaatga
    16921 cctgattccg aatgtaacga tcaccgggag tgttttatat aaagacaaag acatctatgg
    16981 tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc agcagccaaa
    17041 cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa aaggggaaaa
    17101 agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt ctgtttggga
    17161 tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc agcagcagcg
    17221 tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg atgaaccaac
    17281 aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag aattaagaga
    17341 gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta tttcagataa
    17401 gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga agatattcat
    17461 gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat aaggaggaaa
    17521 cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc agttctacga
    17581 aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca tcaaacacga
    17641 taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca tggaaacacg
    17701 cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa ctgatttgcg
    17761 tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg atcatgcggt
    17821 gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa
    17881 agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc tagttgctta
    17941 tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa gagtcaatga
    18001 ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc ctgagacagt
    18061 catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg gagactatgt
    18121 gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg agttaaatac
    18181 gaaccacaat gagattttct aaactaaagc ggtgattctg ccgtttgttt aggaaaagag
    18241 gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga acagtcaaac
    18301 aaaaatagct cctcatattt ttcatttggc tggacttatt accgcagatg caagcttttg
    18361 aacactgttt attcgggaaa gggtttgtga caaaatatgt cacaaaccct tttcatcatt
    18421 tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt ttttatttgt
    18481 gtgcttactt catgggttat tcagcatcaa gctgctttat atgaagtacc tgtcatcgag
    18541 atcgaaaaag tacgaataaa agaggatcat caattgatta caggaagact gatgaacacg
    18601 aatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca aatagaaaca
    18661 gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga atggcagcta
    18721 gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat tggcgccatg
    18781 ttgcttattg gcggaaaaca tggtctattt gctttaggaa gtctcatcat caatatcgtt
    18841 ttacttttac ttttgttgct catctttact aaaatagaag ggatttctct attaaatata
    18901 tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga tggatggaat
    18961 agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc attttttatt
    19021 tgtcttttga caatgaatct ttggaaagac caaggactgc gctatgagga actgaattat
    19081 ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac agctggagga
    19141 acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa gcgacaacca
    19201 gaaataacag cgaaacagtt atggctttcg ggaaggcaag tgggtcgaga tgtgattggc
    19261 tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc gcttctttta
    19321 tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc attggaattt
    19381 ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc ggtcctcttt
    19441 tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt gggattttag
    19501 caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta ggcttattca
    19561 tcaatttagg gatctttttc ttgttgttat tcggttatca tcagaagtgg ccgatactgg
    19621 cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt atcaacggtt
    19681 ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt tgtttcctac
    19741 tattgcttcc tatcactgat tttttagcaa tccaaggttt tacaacgata gaactagaag
    19801 aattatctgg cttagataag actttagcga ttgattttag attacttacg agatcgttgc
    19861 ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct agtgggacat
    19921 tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat tctagtttcg
    19981 ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca ttcatgggaa
    20041 gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag caagtgatca
    20101 atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg tccgcactac
    20161 ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag aaagaacaac
    20221 tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa tcagtgcata
    20281 atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt tccatgaaag
    20341 aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa gaaggcttag
    20401 atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa gaagcagaca
    20461 aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat aaacttgaaa
    20521 acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga caagatcaag
    20581 aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact tcggcgcgtc
    20641 tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag aaacaggaag
    20701 cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa gaattagcac
    20761 agcgtcagac gttagaagca gaaatcaaag agttagaagc tagtggcgat acactgttag
    20821 aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa tggtctgaga
    20881 aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat caagcaacag
    20941 atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta ggaaaattct
    21001 tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa tggaaagatg
    21061 tcagcttgcg tgtccctggg attgcgacaa cgaaatttga gcatgaattt tactatgaag
    21121 ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg ttaaaaacgt
    21181 gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa atgggtgctg
    21241 aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat catatctcct
    21301 tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct aaacgagtat
    21361 acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca ttggaagcta
    21421 aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta actgcaacga
    21481 tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac atcttagatt
    21541 ctgttatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa aacctttctg
    21601 tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg aaaaaagtag
    21661 aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc ggtttggaag
    21721 gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac gaagtagttc
    21781 gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca gatggtgaaa
    21841 tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat acagataaat
    21901 aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt gtaaaatagc
    21961 ttcccctatt ttacaaaatt gtgccttatt gccgataggc tatctcttga acaccgttta
    22021 ccaagagatt atgaacttgc caattatcgc cgagtcacaa ggaataattt caaaaaaagc
    22081 tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcaaaaa gacaccgatt
    22141 aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga ggtgagacat
    22201 gatgaaaaaa aagttgacaa aatccaataa aaatgttgtt ttgacaggga ctttagcagg
    22261 aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg tattcctgag
    22321 cttagttgct tttggaagtc caatcatttt atacatttta ttagctttga ttatcccatc
    22381 tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa aaaacaatta
    22441 tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa tcgatgatga
    22501 cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac gttaacattc
    22561 atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg gacggccgtt
    22621 cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt gacgatactg
    22681 tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa tgcagcgatt
    22741 cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc attcggtgca
    22801 gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga acataacagt
    22861 agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta gaatcaggaa
    22921 tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg tagtactttt
    22981 ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag catgaatgtc
    23041 agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg tgacaggcga
    23101 cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag gactagagtt
    23161 gactggttat tttaattatt attcccataa tcgtttacag ctatttggca gtaaagaaat
    23221 cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa gattatgtag
    23281 tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag aaatgatcca
    23341 ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt ctcgcttatt
    23401 aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg tccacggtgt
    23461 tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga taggaaaaag
    23521 tgaaacagcg ttggagttga tcaaacgcgg tcatcgattg atcgcagacg atcgagtaga
    23581 tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc tggaacatct
    23641 gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg cgagtgcggt
    23701 acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa aagataagaa
    23761 atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtag gcattccaca
    23821 aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag tagccgcgat
    23881 gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa acatttgaag aaagattaag
    23941 tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat gttagcacaa
    24001 atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc attgatcatc
    24061 gtatcaggta tcattatcgc catgtggctt agcagcagag aggctgttcg ggtaggatta
    24121 aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat tattggcgct
    24181 cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat ccaaatattc
    24241 aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc gatcacgctg
    24301 tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat cgctgcacca
    24361 agtgtattat tagcacaagc aatcggtcgt tggggaaatt tcatgaacca cgaagcatat
    24421 ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt catcattgat
    24481 aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc actttggagt
    24541 ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt gaaaaaaggt
    24601 gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat tgaaggatta
    24661 cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt atctgctgtg
    24721 ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa tttaccggat
    24781 tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg ttaaaaatga
    24841 aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac caggctcttg
    24901 gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga tttggggaca
    24961 tcgtgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt atttaccaga
    25021 tcattgtcta cccgattcca tccaagcatt caccaatatg gaagatgcag taaaagacgc
    25081 agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa aagaactagt
    25141 gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag aacaggggac
    25201 acataaaagg atctctgaca tcttgttaga ggaaatccca gtagaaaaaa gaaaagctgt
    25261 tgttgttctt tctggaccaa gccatgcaga agaagtagcg aaacatgaca tcactacgat
    25321 cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt tcatgaataa
    25381 ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg cagctttgaa
    25441 aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg ataatgcaaa
    25501 ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag caatgggtgc
    25561 caacccgctg acctttatcg gattaagcgg agtaggcgat ctagtcgtta cttgcacaag
    25621 tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc ccctagaaga
    25681 ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag cagcgatgga
    25741 actagcccaa ataatggaaa tcgaaatgcc catcactcaa acaatctatg aagtgctgta
    25801 tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg ggaaaaaaga
    25861 aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt caaaaaagca
    25921 gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc tatggcaaag
    25981 gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga agcgttgaaa
    26041 tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat cgaagatcac
    26101 tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac cgaattattg
    26161 aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc tcatccaaag
    26221 ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc atttgtcgtt
    26281 atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt aatcgatgat
    26341 tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag ttcctcatga tgaaacttca
    26401 aaatacggaa ttatcaatcc aggagaagta ttggaagatg ggttatacaa tgtcaaaaac
    26461 tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat tggacgttac
    26521 ttattgactc cagaaatttt tgatgtattg tccgagcaac aaccaggagc cggaaatgaa
    26581 atccaactaa ctgacgcaat cgatacattg aataaaacac aacgcgtatt tgcccgtaaa
    26641 ttcacaggta aacgttacga tgtcggtgac aaatttggtt ttatgaaaac aagtattgaa
    26701 tatggtctaa cacatccaga agtaggtgca cctttacgtg agtacatcct gtctttaggt
    26761 aaaacattag aagcagaagg aagcaagatc gatcataatc caaaaggaat aacagaaatc
    26821 aaagaagaaa agaaaaaata accctttttt ctaacgagct tcagctgcta attgaataaa
//
LOCUS       DACEOE010000019        26872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEOE010000019 DACEOE010000000
VERSION     DACEOE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.020"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            327..890
                     /locus_tag="IW774_000706"
     CDS             327..890
                     /locus_tag="IW774_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153077.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGEFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
     gene            877..1761
                     /locus_tag="IW774_000707"
     CDS             877..1761
                     /locus_tag="IW774_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAQ1153078.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            2155..2652
                     /gene="dfrG"
                     /locus_tag="IW774_000708"
     CDS             2155..2652
                     /gene="dfrG"
                     /locus_tag="IW774_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1153079.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2724..4676)
                     /locus_tag="IW774_000709"
     CDS             complement(2724..4676)
                     /locus_tag="IW774_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1153080.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4683..4979)
                     /locus_tag="IW774_000710"
     CDS             complement(4683..4979)
                     /locus_tag="IW774_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153081.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            5545..5799
                     /locus_tag="IW774_000711"
     CDS             5545..5799
                     /locus_tag="IW774_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153082.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            6196..7797
                     /locus_tag="IW774_000712"
     CDS             6196..7797
                     /locus_tag="IW774_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1153083.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            8031..8330
                     /locus_tag="IW774_000713"
     CDS             8031..8330
                     /locus_tag="IW774_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1153084.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            8464..8841
                     /locus_tag="IW774_000714"
     CDS             8464..8841
                     /locus_tag="IW774_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ1153085.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLIC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            8890..11253
                     /locus_tag="IW774_000715"
     CDS             8890..11253
                     /locus_tag="IW774_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1153086.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGSYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
     gene            11571..12170
                     /locus_tag="IW774_000716"
     CDS             11571..12170
                     /locus_tag="IW774_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153087.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            12282..12506
                     /locus_tag="IW774_000717"
     CDS             12282..12506
                     /locus_tag="IW774_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153088.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            12858..13094
                     /locus_tag="IW774_000718"
     CDS             12858..13094
                     /locus_tag="IW774_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153089.1"
                     /translation="MLSDYKEELKLIENRVLGFVECKDNLDDTLEVLESIIFNTKVLD
                     LKAINGDMTTHIILDEISAKEIGETLIAWANKEL"
     gene            13174..14028
                     /locus_tag="IW774_000719"
     CDS             13174..14028
                     /locus_tag="IW774_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1153090.1"
                     /translation="MMKSNFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            14041..14478
                     /locus_tag="IW774_000720"
     CDS             14041..14478
                     /locus_tag="IW774_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153091.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            14496..14795
                     /locus_tag="IW774_000721"
     CDS             14496..14795
                     /locus_tag="IW774_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153092.1"
                     /translation="MDHKSNYSYKENYIWKYVVAVFLLVGGIIALVKWAIIPNALVFI
                     NWFVPNFWSFFGYILIIWFMFKVLSFFWSPVYSLLGKWSYLILIATAIYIIMQIN"
     gene            14798..15241
                     /locus_tag="IW774_000722"
     CDS             14798..15241
                     /locus_tag="IW774_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1153093.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLSHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSVVFYT"
     gene            15386..15709
                     /locus_tag="IW774_000723"
     CDS             15386..15709
                     /locus_tag="IW774_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153094.1"
                     /translation="MARSRIAKIEQTKKDKKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKNKG
                     IMIPA"
     gene            16127..18937
                     /locus_tag="IW774_000724"
                     /pseudo
     CDS             16127..18937
                     /locus_tag="IW774_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19033..19788)
                     /locus_tag="IW774_000725"
     CDS             complement(19033..19788)
                     /locus_tag="IW774_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1153095.1"
                     /translation="MSNYYHLLNKLEDLNLKHTREILPEYLDTIVQENLSFVEALNHL
                     MTEEINSRNERQKENRLRKANLPFKKTINDFDFGFQPKINRTEILDLCTLRFMDTNNN
                     ILFIGNSGVGKTHLAIAITLEAITKGYSCHFALSNELVNRLNRANQRGNLDRLLKKYA
                     SYDLLIIDEIGYLPFTPDGAKLFFQLINLRYEKKSTIITTNIPLSQWAETFQDKKLTN
                     ALIDRLVHHAKIIQITGKSYRIKDYKEKKLSYK"
     gene            complement(19803..21029)
                     /locus_tag="IW774_000726"
     CDS             complement(19803..21029)
                     /locus_tag="IW774_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1153096.1"
                     /translation="MRKDIYEGVLPFIMTHTKPNYAALAKQYNCDYRTVKRYYEAGIS
                     NKLPLLAQKNKNKKTLVSDFEDIIADKLALGCSSSAIYHFISKRGYRGSARTLRRYCR
                     NIKADKIQKATIRVETTPGLSAQVDWKEDLKLVNKFGEVISCNIFLYVLGYSRIKYLE
                     LTFDRSQTTLFRCLTNAFQYTGGVPTEIWFDNMKTVVDRSRSQFSKVIFNERFRQYAK
                     EAGFLPIACRAFRPQTKGKVEALARTIERIRVFNYEFESTHELEKIIQTIMDSLNTEE
                     ISQAIHECPSDRWSYEKEQLSSFDANQLKIYASVEKQIKRKVSNEAMVQWNQNKYSVP
                     VNYIGKILTLRVKEDKLYIYDDSTLIRTHTMTHQLLNYHRNDYIEILRSDVFKHLEDE
                     ELERFVDENLQAYDEL"
     gene            21137..21964
                     /locus_tag="IW774_000727"
                     /pseudo
     CDS             21137..21964
                     /locus_tag="IW774_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22024..22386
                     /locus_tag="IW774_000728"
     CDS             22024..22386
                     /locus_tag="IW774_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153097.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSIVFTDMVHKMLNGEPINFNFDYMLEKAQSNQIVAFIVSIYYFIEKSIFSLKDF
                     FFEMLTKLTTFYNKISKN"
     gene            22620..23042
                     /locus_tag="IW774_000729"
     CDS             22620..23042
                     /locus_tag="IW774_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153098.1"
                     /translation="MIYVAFFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETAD
                     YKFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            23054..24469
                     /gene="cpaF"
                     /locus_tag="IW774_000730"
     CDS             23054..24469
                     /gene="cpaF"
                     /locus_tag="IW774_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1153099.1"
                     /translation="MRVVEEKKISPKIEETPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSASEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            24486..25301
                     /locus_tag="IW774_000731"
     CDS             24486..25301
                     /locus_tag="IW774_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153100.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYLNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            25301..26098
                     /locus_tag="IW774_000732"
     CDS             25301..26098
                     /locus_tag="IW774_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153101.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKKDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIRPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            26116..26478
                     /locus_tag="IW774_000733"
     CDS             26116..26478
                     /locus_tag="IW774_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HAQ1153102.1"
                     /translation="MQEQSVMEIIKWFIGLMMIMAMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDRRFTIESDKLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVTLSFNGNSVSQIR"
     gene            26650..26871
                     /locus_tag="IW774_000734"
     CDS             26650..26871
                     /locus_tag="IW774_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153103.1"
                     /translation="MEAIQENRKDNQETSEKNKKKRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
BASE COUNT         9886 a         3507 c         4859 g         8620 t
ORIGIN      
        1 agaattataa tcacaaactt aatagtaatt actattggga ttacaacatt aacaaataaa
       61 gctttaatat caagtatgat tttaatagtg tttgatttta ttatcattta tagtttgctg
      121 tttttaataa agaaagtaac tgaatattta aatccgttag gcacgaaata aattttaatt
      181 gaggttgaat tatagcggta aatcatacaa aatagaaatg tagtcttgtt aagcgtcttg
      241 actatctaat tttgaatgag gagggttgta tatagaaaaa gggtagttaa attcgtagat
      301 caaaaggcta cgaaggagaa taaagaatga cagctattta taaagcacta aaaaattcta
      361 acgtatcaga gtacgttaag tcgagagtat taaataataa tttagattac attttagaaa
      421 atggtgttgc tgatttaatc agtaaattaa gtgatcctat agatatagat catttggtgg
      481 tatggcaaga tggtacttta gaagtatcaa tatatggaga attttatagt agaaataaag
      541 taactacatt caaaaatagt attagtggtc gagaagattt aaaaggaatc aataaaaata
      601 gtaaattcta tagaacaaat aaagaattgc tagaaaaaat ttcaaagcgt gtttttgaat
      661 tggtgaatcc attttcaaaa aaacgtagta aagaactttt tggaattttt tatgatggtt
      721 ttagtgcaaa aagtaatgta gaaaaatttt tttattttaa tgattcagct tatattgcgg
      781 ttaaagacag taaaagaatc cctgaagcaa ctttagaata tttagagcca attaaattgt
      841 ctgtttttta ttcactttat gagaaagaaa tcgctgatga agcggtttaa ggggagtaaa
      901 atgagtaaaa aaataggcaa agagtcatta tcgctcggat tgaaaaaaga aattgttaaa
      961 tttaaaagta tgtatagaga gccggaattt agactcattt ttcaattaga tatagtgaga
     1021 gggctgaaaa ttagttttag ttgtttaggt tatctggtct ggctattcat tgctagagta
     1081 ggatggagca atgcttatag cttttataaa ctgttcgccg gaactgatcg attaaaagaa
     1141 tcattagagg tgaaagaagc cttaaatatt ttaggaagtc ttgctttcag cctgtttatt
     1201 tttgctttat ttatcttaca agtaggaaaa ataatgatgt caacaaatga caattatatt
     1261 aattatttga agccttttca aaaaggaata aaaatcattt gtagacaggg cacagtttta
     1321 tttctactta gcctgtatga gcatgaaatt cattttcaaa attattataa tacgacaact
     1381 aaacttggtg catttttact cctatattta gttagtaata tagctgaaaa aatgctgttt
     1441 aaacatattg taaagcaagt cttttctgat attcaaaagg aaagctattg tttaaaaaca
     1501 ccttatggaa atttaaaaaa tgttgagctg tttttattta aaatggatag taagtgggat
     1561 aaatatatct atagaagtaa agaagtattt ttaactggta gttataacaa aactcttcag
     1621 gagagcctgt acgacacagg agcaatattt aataatgaac ctatggtttt gaaagatttc
     1681 tttggagatt atgaactagt ttggaatcca aaacagctta gtgaacaggg ggcagaaaat
     1741 gaatttgctg aagttcacta acatagtgta cagaaaaaca ttatctgaaa gaagaacgcc
     1801 ttcaaaagaa agtacaagaa cgattgggcc gtacagaaga ctaacgaaag atcgtaaaaa
     1861 aaaggaaaga caatgaaaat taagtagttc agttttggag tacaaaatga caaaatgtac
     1921 ttcaaaatgg actacttttt tcattttaac ttacatacca aattacatac aaaatgattt
     1981 ataaaatgca attgaaaatg ataattgaat gaaaatttgt gattttgtgg tattatgata
     2041 atataactta tagaaaaaat tgcttattca actaacgggc aggataatga gaaaaggttt
     2101 tttgatagaa ataagaaatt agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa
     2161 gtttctttga ttgctgcgat ggataagaat agagtgattg gcaaagagaa tgacattcct
     2221 tggaggattc ccaaggactg ggaatatgtt aaaaatacta caaagggaca tccgataata
     2281 ttaggtagga agaaccttga atcaatcgga agagccttac ctgacagaag aaatattatt
     2341 ctgacgagag ataaggggtt tacctttaat ggttgtgaaa ttgttcattc aatagaagat
     2401 gtttttgagt tatgtaaaaa cgaagaagaa atttttattt tcggaggaga acagatttat
     2461 aatttgtttt tcccttatgt tgagaaaatg tacatcacaa aaatacatca tgaattcgaa
     2521 ggagatactt tttttccaga agtgaattat gaggaatgga atgaggtatt tgcccaaaaa
     2581 gggataaaga atgataaaaa tccgtataac tactattttc atgtatatga aagaaaaaac
     2641 ttattgagtt aacgggtgcg ttaattgaag aagaacagta aagataaagc acaagagctt
     2701 gggattactc caagctcttt ttgtcatttg aaataaatta tatctttcca atcaaattgt
     2761 ttatctgtaa tccctaatct ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa
     2821 gggcgacaaa agttgtagta agttcttaga attgtaatcg caaattgagc atacttcgga
     2881 ttgaagttag cgtaaatata gctcttctta tcaccacgag ctgttgttaa tggtctttca
     2941 agagatgata tataacgtct tatctgttga ataaagctat tggtcgaatg gtcattaaca
     3001 tttaatagca tctttgcgat ttcgtttggt tccagagcag ataaatccgt tctacaatca
     3061 actgaataaa atcctttatc cttagtggct aatggatgtt tgatagggtt atcggcatat
     3121 ttgcgatatc tttctccatc tttactaata gcctcttcgt ggaattgatg acttgttagt
     3181 aattcagtta gataatgaga tgccaaagtc cgtagaaact ttgttttgaa tcctctgata
     3241 tctccccaat cgagcagttc tgcttttgct tgtccgaatt cttttaagca ttgttttctg
     3301 cttttcgtct tgttaacttg gcaaataaaa tgatgtgcat cgctcaacct taattctttc
     3361 gtgaatactc tataaaaggc gtttctaata gagaagtcat catcactaat cattcgccat
     3421 tcgtcggcat taaccatttg tttaattaac caatagtgag cagttgtagt ataaggagca
     3481 tcaacattta aaccatcgac aaatctactc ctattagtaa ttttccctaa ttcgtgaaga
     3541 taagcattcc tattttcgct atcgttagcg gatggttctt gtggataata agaccaatct
     3601 aacctatcat ttttacgact aaaagtattg agatggtctt ctttgaattt tcttgtatct
     3661 tcatttaatt catccattga aatattccaa tcataggcaa catcagaacg gaacacatat
     3721 cttgataaga cctcagcaga tacaactata tacgtttgca tattaaggtc ttcaaacccc
     3781 gtatatttct ttgaaccttg tcctttctta cggacgttat ttaggtagta gtgcatcttg
     3841 tccgtattaa gccacatctc gttaaatttc tttgtttgaa ggggttgtgt ctcatatcgt
     3901 tctaaaaact ccaaacaacg cctatacagc cactccagct tatgatagta ggttccaact
     3961 ccaattccta aaatatcaca agtacgactg actgaaaccc ttccaacaac catcttagcg
     4021 aacattggaa gaatggtgtt cttttgttgg tgataagtag tggtttcttc tctttttggt
     4081 agaacattag tgaacttctt acacgcctta cattgatagc gttgagattt gcccctactt
     4141 ttcccccttt tgtaaaattg cttcggctca ttgaatgggg ttgattcttc ttccgagcag
     4201 ccttctttat gaaattgata atctggctca acatcttgga tgctattgat ttctataagg
     4261 cgtttaattt cttcaacaac agaccaatta gataaagggg ttactgtaca gttttgcgat
     4321 acacctctgc caataggatt gtcattgcag tacatacctt tatgaccttt atctttggaa
     4381 ctaccagtca ttttatatcg acttggtttc ccttttacat ctttgaattt gtgctgttct
     4441 ttcccaaaat tacaacaaaa cggattgatg cagtaattat attgaatggg atattcagaa
     4501 ccgttccatt taaaagacgt tggcaagaac aacattttgt tataccttgt tgcaaatcgt
     4561 tttggagtta ataaatcata ttgtttagcc ctatctttta attcttcgtt tgaaatcgga
     4621 attttaacat ctacaatttc atcttccttc gtggctaatc ttttaagctt ggtcattgtc
     4681 tatcaaccca ctctatcttt gatacccatt ttttcaacaa tccgcttatt acttcgcttg
     4741 ttggcaatcc attcaataaa cttcttatcg tctaaaatgt tcaataagaa ttcatccact
     4801 gcttcgcttt cagtacgctc tgcatattca gcgtattgct taactatcac ccttacttgc
     4861 tctgatattt cccaatccac agacttagcg ttcttatttt ttggctcaag aaattgcata
     4921 ggaaaatgca cccctttttg cttagtaaat tgtaaccatt ttaacacacc aatttgcatt
     4981 cctacaataa ttatttcacg ttcgcatttt tatatacatt ttaacttaca ttttgtggtg
     5041 taaattaaca acaaacgttg atttaacaat aaaaaacgag tatcaaattc agttgcaatt
     5101 tgatactcat ttaggtttac attttcactt tttgtactcc aaaactgaac tacttaaatg
     5161 aaaatgtctt tccttttttt ttgactacaa gtagcctgaa aatgaaattt caaaagacta
     5221 caagtagtct tttgtggcaa aaataaagag ccaacgaaag ctaccaacaa tcgttgactc
     5281 gtaagactgt tcgtagtaag aaaaatcttt ggcaatccac gaagtcttta tttcataatt
     5341 tatataaatg attatacagt cactttcctg aatttgcaag taaaaaagct aagaaatatt
     5401 gtttaagcgt ttccttaaaa gaatctttaa ataaattttt ttccaattga aaacatgact
     5461 acaagtagac tatttctttt attccaagtt gactacaagt agtcaaggtg atataatata
     5521 tatatcaaga aaggagaaac aaaaatgtat aacgtaggag atcaaataga tgagatcgta
     5581 aaaataggta atgttaaact tgttaaaagt aagtactata tttcagaaac tgaaataatt
     5641 tttaatactg aatcaggatg tattctaatt tggaaactgc cttttcccaa agacgaaaca
     5701 acactgaaaa aaataaatac tttaaaaatc agatattgca ttcatgaaat tattgataaa
     5761 aatacttatg ttataaatca agtaaaagtg atggggtaaa aatcttttaa aaaaactctt
     5821 gttaaaagta attctattag cttataatgt tggtattagt tctgaagcca aaggaacact
     5881 aaaaaattct agtagaaaag ttagttacag actctctact atgtatgaat gtaacgaaaa
     5941 aaatagcgaa aaagataatt aaataagact accaatctta tttaatggaa cttctgtagt
     6001 aagaaaagcg atttggcaat ccacgaagtc caccaagatg agctaccaac tcattttaat
     6061 ttttgcacgt ttaaagttta gcatttaagt gcttactcta aagacatagt gtaattttag
     6121 gactccttat tacggaatct aaagttaccc tatgtctttt ttgctattca aaattgatag
     6181 caaggagaaa gtgcaatgtt gaatagttgc gagtatgcgg acgttttagc taaaattctt
     6241 aaaaatggaa ttaaacagtt taatagcaaa aatctagttg cttttaaaaa gaatagcaat
     6301 aagattggtg ctattgccgg atttacttct aaaacaaaca tgataaagac taaaggtctt
     6361 atatatcgtt ctattgatcg tgtcttggaa gattcagatg tattaacaca ctggactcct
     6421 aacgtgtatt catggcttgg tggcggtgaa aacaaaacaa tttttggtca caaagaagct
     6481 aacttaattc agattaatac ttttgtagca gatattgact atccagtagg agaaaagaaa
     6541 acggatttga atttactagt tctttatcta ttggaagaag ggttattgcc tacactgatt
     6601 gtagatacgc caaaaggtta tcattgctat tttctgattc aaaactataa ccaagaaact
     6661 gattcatttg ataaggcaag ctatatttct aattcaaatg attttaaatc tttgagagtt
     6721 gctaaaagaa tatcagaaaa tatcagaaaa tctatccaaa atcgtttacc acaagtcgat
     6781 atgggctgta atcattttgg tatttttaga tttccaacta aaaaaaatat tgtacattat
     6841 gaacctaatt tcgtagatac gtttgaaggg tatttgaaat ggtcaaaaga ctttgaagaa
     6901 aaagaatatg ctttaaagag gtctaacttg tcattattag ctcctaattc tactggcaag
     6961 aaagggtcta aacgacaaat tgatactaaa tggtacgact ttttaattca tacggatatt
     7021 gctgaaggag atagaaataa aactattttt accttagccc tggcatgtaa atcaagtggc
     7081 ttatctattg gcgagtgtat ggatcaattg gatcaacttc aattcaataa tgatttgtcc
     7141 tattctgaag ttgagagaac aataagaagt gcttatcgtg gcgattacaa aggtgctaaa
     7201 agttcatatg tcaatgaaat tatcgaaaat tatgctacac ctaccgagtt tgaaaaatac
     7261 tcaataaatg taaatagaca agctgttaaa cgtaaaggga atcgtgtcga tccgtctaat
     7321 tgggtgaaat ttgctaaacc tagagaagaa cgaaaatata gtcattttga agaaagtaaa
     7381 gctgatttat tagcttactt agaaagaaaa caaagatcat tgaaaaaaaa tgagttgttt
     7441 ttaagtgtct ctatgggaac tatatctgaa gaaacacaaa ttcctgtatc tacgttaaag
     7501 gtgattttaa aagagcttag aacggcgaat acagtgatat tacgaacgtc tcgtggtcgg
     7561 tatggatcga ctaaaatagc aacaagcaaa tttgtggaag ctaaaatatt aaatacagtt
     7621 attcagaata aaaaagataa acaagtattt gtcttagaat tattaggaac agcaaatagc
     7681 cacgtagaaa cgattttaag acgttatgaa gacaaaacag taccgttcca tgcgaacata
     7741 ctgaaagaaa gagaaaagag caaacatgcg ccaattagag gagctaatac aggttagagt
     7801 tctatttatt tttttgattt ttaaatagct aaattgccaa ttaacatatc tagtgtttct
     7861 gcccactcct attcctgttt tagcgtatac taatgattag tagttagtac tctcttttat
     7921 gtatggagtg tatagaacta tttttacttg tgtcttttcc cttaatccac gtgtacggaa
     7981 tacagcttat aactgcgtat gcagttatgt ttgaaggagg aagattttct ttggtaatta
     8041 agaaatatgt ttactcacac ccgatcaatg tatttattat cggtaagcta ggtttatctg
     8101 ttgaggtgtt ttgtgagtta tatggattta aacaaggaac tctatcgtct tggattacta
     8161 gagagcgtga ggtagctgat ttgcctattt cttttttgta tgctttgtct ctagcttcag
     8221 gagggaatat gaatgaggtt tatgattgct tgtgtgagta tgaaaaagag tataaagagt
     8281 atttgaaaat ttcagcccca aaacggaata aaaaatatat tgaagattag agaaagaaga
     8341 aaagaataat ttgttttttg actacaagta gacagatagc tactctgttt tttgatagtt
     8401 tgatattggc tgtttttttg ttagaattaa tatatatcag gtaaataaaa aagggggcta
     8461 ttaatgaata ttgatgggat ttataatgaa tcagatttta aaaaaggcat agtagatatt
     8521 gatagaaaag agtatttatt tcctgatgaa ataggtttat ttaaaattgt aggtgaaact
     8581 gtctgtaggt attgggggaa gaaaggaaat ataattttac ttgttgatac gattgaggga
     8641 agaaagttaa aattttcgtt ttggagtaat acaaaatatt gttgtgaacc tgtacctaaa
     8701 agggaagtag atgtggctaa agaaagtgta cgatttttac ctgatcgatc aaaaataact
     8761 ttaatttgtg aacggacaag taatgctaaa tttacaagaa ttagagatat actactttcg
     8821 actacaagta gtcagtcata gagtaagaac ttttggttaa aaatcaattg aagaaagtgg
     8881 aggaagtata tggcagtagt tatattagca gaaaaaccag atcaaggagc aaaatttgct
     8941 tcagcattgg ccggaaaaaa attatcgaaa aagagtggga agtatgaatt taaaagtgaa
     9001 atatttggag atactattgt tacttgggga ataggtcatt tggtaggatt atcattacct
     9061 gaaaagtatg agtggctacc gaataaagat aaatggcatt tagataattt gccttttcta
     9121 cctaaattca atgaattaaa gtatgaagtc tctaaaggaa aaaaccaaca attttcaata
     9181 gtgaagtcat gtttagaaaa tgcagatgag ataataattg ctactgatcc ggatagagaa
     9241 ggagaaaata ttgcttataa tattttcaaa ttatgcaagc agtcgatttt taataaaccg
     9301 ataaaaagat tatggattaa ttctttggtt caaaaagaaa ttatcaaagg atttaaaaat
     9361 ctgcgaaatt ctgaagaaac gatctcttac ttcaaagaag ctaatgctag acaaattgct
     9421 gactatctag tagggatgaa ttatacagag ttgtattcat taaagttgca agaaaaaggt
     9481 atatcgggtg tggtatcact cggtcgagtt cagacaccgg taaacacact tgtggttgaa
     9541 aacgatttgg caattagaaa ttttagaccg gaaaaatata aaacgattga atgtcataca
     9601 accgataagc aaccaaaagc aatatttaag aatagtcttg aatactttaa attagaaaaa
     9661 tttgaagaag acactaaaaa attcggcctt gattccgcca gtactggaat cataaaaaaa
     9721 attgaggtta gtaaaaagca aacagaacca caaaaattgt ttagcttggg tggtattcaa
     9781 gaatacgcta ataaaaaatg gaaatattct gctaaaaaaa cagataaaat tatccaaagt
     9841 ctatatgatc gtggatattt atcttatcca agaaccgata gtgaattgat aactacaaat
     9901 gaatttgagt atttaaaagc gaatttagac gtgtataaga gtgttttagg gatcagtatt
     9961 gatacacctt gtttagagcc taaaaagcgg tttgtagata atgacaaagt attagagcat
    10021 tatgcactaa taccaacaat ggatattcct gatatagcaa atctgacatt agatgaaaaa
    10081 aatatttatg agatcgttac taggcgtgcg tgtctaatgt ttgcagaacc ttacaagtac
    10141 gaaaatacaa aagttacatt agatgtaaac ggcttagaat ttaccacaag tggtaatgta
    10201 cctatatctt taggttggaa gcaagtagat actaatttag atgaatctga tgataaagag
    10261 gaaacattac ctaaatttca tgaacaagag acaataaata tagatattag atttattgat
    10321 aaattaacta aggaacctaa aaggttgact gaaggtaaat tattaggtaa aaccggtatt
    10381 atggcgaaat tagggctagg aacgcccgct acacgttcta atattgtaga cactcttcta
    10441 aatagggaat atattaagat agaaaacacg aatgtttatc ctactccaaa gggatattta
    10501 ttatgggatt tgacaaagaa taaagaccta ttgattggaa aaccagaaaa tacagcgata
    10561 tgggaagaat cacttgggaa aattgcgcaa ggtagctata cacaggaagc ttttttaaga
    10621 aatatctata actatatcaa taagactgtt tcagatttga aaaaagtaga atttacttcg
    10681 gattatttag aaaagactgt aaatcaaaat aaatttgata taggtagtta tcaagtggaa
    10741 gaaaagaaaa aagtttattc tattttaaat attactagtg gtgaacagtt cgtgatattt
    10801 aaaaatatat caggaaaagc aatcaataaa aaagttcttc aagaactatt actaaataat
    10861 cgtacagata aagtcctttc cggctttaag tcaaaagaag gaaaagcttt taatgcaaga
    10921 ttacttttcg atccaaatgt gatgaaagtg gtaatggagt ttgaaaagaa cagtccaaaa
    10981 ggcgataaaa agagcaaaca cgatacagta aatggttatg aaatagttga aaaagcaaaa
    11041 gtatttgaaa taaaagaatt agcaacaggt gaaagtttta ttttctataa aaataatagt
    11101 gggaaaactt taagttcaaa aacagtcaaa gaattgttag aaaatgggaa aactaaagag
    11161 aaagtaacgg gttttataag taaagacaat aaaaaatata gtgcttatct tgtctttgat
    11221 aataaaaaca aagttataaa gaaagaattt taattcttag tggcggaaat catttgtttt
    11281 tccgcttatt tttattcaaa agtgcccaaa atataggtat actgaattaa ggataaatac
    11341 aaaactagtt agaggacagt tgaaaaaata caaacaaatt agattgctat tttgttaaat
    11401 tggaagtaga ataataccgt tttgatatgt atagtgtgtt ttaccaataa aattacataa
    11461 acgtgaatat atttttatga attaaatgta aaatgagagc aattagtttt aaaaaagatt
    11521 ataggctgtt aatcttagca tatataatcc attggaaagg gggagatcca atgaatctaa
    11581 agaatacaaa ctcaaacaaa tctacactat atgtaatttc agatgattat ataaataagc
    11641 tgaaaaaggt ggactatcgt gtccaaaaca actatcgaga acaacgatta tattttaaat
    11701 caaacttgaa aatttctaac aaaaatgaag aaataaatta ttatgtacca ttggcttcag
    11761 caaaagaaaa tcaacgttct ttgactaata aatcagtgtt taagatttat ggtgatcgag
    11821 aagaaaagga ctttttagga gtacttcata taaataatat gattcctgtg ccaaaagatt
    11881 gtgcaaaagt atggacacct aatagcccaa atgttgatga gaagtataag atgttagtaa
    11941 tcaaacaggc aaaatatttg aaattacatg agtctgaaat aaatgaatca tgcgaattac
    12001 tttataattc taagttaggt aaacttgatc ctagttactt taagaaaaat cgtaaagaag
    12061 taattactta tcaaaaaata atgacgaatg tagaatcatt ggaagctgtt agtatagcag
    12121 aatcccaaag aaatattcat gtacaaactc aaagtataga tgtagagtaa gataggctaa
    12181 acctatctta ttttattatt ctattggata ttatgataca ttgaatttta atcgaatatg
    12241 tatttaaatg ttattatact gtagaagata aaacttaaag gatgatttct atggcgagac
    12301 cgagaaaata caatacagaa gaagaaagaa aagaagcaat gaaaaaggct aggaaaaaat
    12361 gggttgaaaa aaatcaggaa actattgcct atataagagc caaatcattt gctaaaaaat
    12421 tcattgaaat gtctgaagat gaagatattg aattgttgga aaaatggtta gaagatcgga
    12481 aaaataacgg tcaaaatgat gaataataac cattgactac aagtagtcaa tggtgtagaa
    12541 tggttttagg gatagaaata tcccttttct aaaagtctaa tgactacaag tagtcgatag
    12601 agggatcggg aataaaaaca gacgattatg gcctaaagaa aggaggaata tgaatatttt
    12661 gaggaacgat ttattaaatg agcgttgata aatttcacta aatattttct taatatctat
    12721 gtattgttca agcgtagaaa aaaatattga aattattaca aaaccgctca taatggaaaa
    12781 taacgaatta tattaatgaa tcgttcactt ttgacctttt ttactagatt tttttgtagg
    12841 acttttagag aatttttttg ttatctgatt ataaggagga gctaaagttg atagagaata
    12901 gagtactggg gttcgtagag tgtaaagata atttagacga tactttagaa gtccttgaaa
    12961 gtattatatt taacaccaaa gttctggatt tgaaagcaat taatggtgat atgacaacac
    13021 atatcatatt agatgaaatt agcgcaaaag agattggcga aactttaata gcatgggcta
    13081 ataaagagct gtaaaagata attttaaaat aaagtggcca attgactaca agtagtctgt
    13141 atagtataat aaattatcaa aagtgaggta tacatgatga aatcaaattt tttgggatat
    13201 gtaattgcaa tcaacatgtt taaaggggga gttggtaagt caactcttac tcagctaatg
    13261 gctgatattc tatcggctag aggctttaaa gttttagtta ttgatgttga tccacaagga
    13321 acagtaacta aaaagtttca aagacaatat gatttaaaag ttgttgggaa aatgacattg
    13381 tttgaagcag taattaatat gtctttctta gactgcattt ctcctgttaa agaaaactta
    13441 gatattgtgc ttggttcaga acaaatgtca aggtttacca aagtattaag agatatgtgg
    13501 agaagtactg gttttactga tgaaggtgaa gaagtctttt ggaatttgtt taattatatg
    13561 atcggtaatg ataatttaag agatcaatat gattttattt tgtttgacac tattcctaca
    13621 gtttcagaat ttacagataa cgtttacgtg gcgagtgatt ttttagtaat tcccacgcaa
    13681 actgagcaag attctataga caacttgcat agtacggtta ctaattatca aactgctgta
    13741 aaagaagtaa acggagattt agaaatttta ggtattgttc catatctagt ggatactaaa
    13801 ttaaaaacag gattagaact tttatcagaa cttcaagaag aatatggaga cttagtttat
    13861 tctcatattg tttcagaacg aggcgtaatt aaacgttggg gtagagaagg actaaataaa
    13921 tctatcccac attctaaaac tgcttatagc atgtatgtag acattgtaga agaaacgtta
    13981 gtacgtttag aaaaagctac aggagaaaat attttagaac gtgtttagga aaggtgttgc
    14041 atgatgtcga aaactaaaaa agtaactagt gccggcagta atggttcggg taatccgttc
    14101 ggaataaaaa gcggtagtgg tggatttaat ataggacaac aaatgttaaa aggtaaagct
    14161 ggcattaagt ctactggtga taaacggaaa tctgttaggg taccagttga attatacgat
    14221 gaagttgtta aagatttatc atacggggct aaatattcat taatgacaga aattttagct
    14281 ttatttttaa aagaatttga aacacatgga aatagtatag ttactgattt tggcaagttg
    14341 aaaacgagac cagtaaaaat ttctgaagaa gcctttaaag ttttgaaagt tataaaattt
    14401 gaaacagatc aatctaatac taatattatt gcttcagctt tgacatggta tcttgataaa
    14461 agaaaaggaa aagcataatg aaatgaggta agactatgga tcataaatca aattattcat
    14521 acaaagaaaa ttatatttgg aagtatgtag tagccgtttt tctactggta ggtggaatta
    14581 ttgcattggt taaatgggct ataattccta atgctttagt atttatcaat tggtttgtcc
    14641 ctaatttttg gagttttttt ggctatatat tgataatctg gtttatgttt aaagtactct
    14701 cttttttctg gtcgcctgta tattctttgc ttggaaagtg gtcgtaccta attttaatag
    14761 cgacagctat ttatatcatt atgcagatta actaattatg cagattttag aagatattaa
    14821 aatttattgg aaaaggataa taggctatac attagttctt cttgtagtaa atttattcct
    14881 ttcgatgata gcgttggtct ttgttaatca aagaaaagac aatgtaactg aattagtaac
    14941 gtttatttcg tttttcataa gtgggatttt tgctcctttc tttgaagaaa ccttaatgag
    15001 aggaattatc caaaaccaat tggaattaaa aacaaatttg tctacaagaa gcatatatat
    15061 aattgttgca attttgttta gtctatccca cttaaattta tattttattc cttattttat
    15121 gacgagttta attttgtctt ttgtctatga taattcagat aaaaggctga ttgttccggt
    15181 tactattcat tgtttttaca atttaattgt aatactgtta agtgtagttt tttatacttg
    15241 aaaaaaatag acgaaaacct aaaaatacaa ggtttttatc tatttttttt attttgtttt
    15301 tatctatcag gttgatagaa attgtcataa atgatataat taagtatgaa ctgatttagt
    15361 aaaaaaatta aaggagtgat agctgttggc aagatcaaga attgctaaaa ttgaacagac
    15421 taaaaaggat aaaaaaattt ctaaaaataa aacaaaattc tttaaagctg aagatgttaa
    15481 gaggatagca tggtctgtga aaacatttga tgaagatcgt tatgttaaag tttcaatgaa
    15541 tggtctattt gtacagcttg gtaacttcag taattttaaa acgattgatg aactttatcc
    15601 ggaaataaga aatattgtgg atagtaaagg tttaatcgtg gctaaacgga aatcaagaaa
    15661 ctctggtctt gcttactgta aaaataaagg gatcatgatt ccggcttaaa tcagtgaaat
    15721 tgttctttga aaacttaata ctactcgtac tgtagcaatg ttgctatgtt acagataaaa
    15781 gcgtaaggct aataagcaaa atagcaacca agcatactgt tatttccttt gccaagaggt
    15841 atgtaaatac gttagcaata gaagtgataa ggttagtatt tcacgccaaa aaatacaaaa
    15901 gtcgatccat tgaaactact aattatgagg tggcgtaaat ggtaattatt cagaatttct
    15961 atctaggaag aacctatgaa aaccgtccta gagatcagaa tattgacact aaaaaattca
    16021 gcacagtaat aaatgtgcta gatacagggt aactctttac tacaagaaat gtagtaaaga
    16081 gttgttatga ttctttatta catttctttc taaaggaagt gtgataatga aaaaactaaa
    16141 actgattagc attgctctat tcagcctaat cactatgtca acggctcgcc aatcaattgt
    16201 ttcggctgaa ggaacgtcta cgaaagcagt agtagaagag tctggcgata aaaaagttgc
    16261 tatagacgac aaagtgaaaa acgaagcgga aaaagcgact gtggttcaag gggcagacgg
    16321 cttgatgtat gtggtcgttg atgaaaatga tcctgaaaaa ttgctagaag ctgaatctcc
    16381 tttgaaaaat caacaagaaa ataccggcgc tgttgaagtc ggtgttgatg aaaagagaca
    16441 agaaaaggct gataaagcta acgaaaaagt gaaaaacttt gacgatattg attacaacga
    16501 tattagttcg ttgcagtcag cgttgaatga tccgaaattc ccttttttat cggaagatga
    16561 attaaatgaa aaattgcaaa agacaaagaa tacatttgag tctgaagcgc taatgaaaaa
    16621 gacaagggga tatttcagtc aaaggatttt agagttaagc aataatttaa atgacgtacc
    16681 gaaaagagcg aaagtgagtc ggcatgcttt agttagtaac cgtgcttcac aagtaagtga
    16741 aacacgaata aaccctccaa atggttatcc gtgggtgatt tatttaatta ggagcaatgg
    16801 ttatagttat acagaaaatt atgctattag aagaactatt gatgggaaag aagctttttg
    16861 tttacaggcc ggtgtacaag cgacgaatac atacactggt tcaacaagtg gttatctttc
    16921 taattcgcaa agagaaatga ttagttcgat tttatcagca tataaagaat taggcggttg
    16981 ggctaataat gatgccggaa aacaaagatg gatagcaact caaatgcttg tttgggaaaa
    17041 cagtaatgaa aagcctaaaa gtataactgt tacgccaaat tggaaaaacg gagctactaa
    17101 gtggaaaaat gatttagtgg ctcgtgcaaa tgctattaga gtaagaacaa aatgggattc
    17161 tacgtctaag acggttgatg agggagataa agcaaccttt aaagtaacta atacttatgg
    17221 agatcataat ttgcatgtga aatctgtatc tggtggcggc aaagctacca taagcaatgg
    17281 tactttaact gttgatacta caggagccac tggaaacaaa atcactgtag atatgattaa
    17341 aggttctcac ccttcagacg cgcaccctac aacggtatgg agtaatggtt cttatcaaaa
    17401 gcttgttacc ggttcttttt acattagtac ttctgttacg gtaaatgtta agagaaaagg
    17461 aactgttgaa gttttaaaag ttgattcaga aacaaaaaaa ccattatcag gagcaacatt
    17521 tagatttagc tactcaggta aaacaaaaga tgttactact gattcgaatg gtaaagcaaa
    17581 attaactgaa aggttgaaag ctggaactgt agtaaaagtt aaggagataa aagctcctaa
    17641 tggctaccaa ctagattcaa gcgaattttc aattacagtt aaagaaaatc aaaatgttac
    17701 taccacaaga accaataaga aatccacagg ttctgtagaa attgaaaaga taggcgattt
    17761 agatggatta ggactaccga atgtagtatt cacgatttat aattccgata acaaagttgt
    17821 aaaagataac ttaaaaacag atagtaatgg gaaaataaaa gtcgatgaac tgcaatttgg
    17881 taaatattat gcagtagaaa aacaaggtgt tactggttat gatcccgatg gtaaaaaata
    17941 caattttgaa ataagtcaag atactccttt tacaaaaccg gctaaagtga aagtaacaaa
    18001 tatttatgaa gtatccataa gcgaaaaagt atcggacatg aatgaaagtc aggtattaaa
    18061 aaataattgg tctgctgatc caaacgatta tcttcaatat gacattacaa ctggaaatct
    18121 aaaaaaatcg ggagaaagaa acgtccaaag tttcattatc gaagggtttt acgacaataa
    18181 gaatgtagat gtgtcaaaaa gtgaagtata tgttggatca acaaatgtaa caaactcttt
    18241 tgacattaat aacgatacta acaatggaaa agtaattgct aaggcaaaga caagcgtatt
    18301 aacgaataat gatttttata ataagtccta taatttacgg atcactatga aaattaaaaa
    18361 atctactctt gaaaacactg aaaagcaaaa aaatttaacg attgttaaaa atagtaaagt
    18421 tactgtcggt tcaaaaatct ctaaatctgc tagctctaat gatgtagaaa ccgttttata
    18481 ttcaagaaaa gttactatca atcatattga tgaaaaagac aaacaccttt tgaaacaaga
    18541 tattgattat aaatacgatg gggaaacgta tgaatataaa cctagaaccg atttatttga
    18601 taaagatgga aacaattata aatctgatgt aatccataaa gggaaaattg atggaaaaga
    18661 tatagtttta aacactccct atcatattcc ggtactttct gtgaatgtgg atcggattca
    18721 aattgatacg gctagagctg ttaaaaatgg ctatttacct acaaaaatag aactaagtaa
    18781 aaaagaagaa tatgaaaaag aattagaaaa aattgtattc aaagttaaaa ttacagatat
    18841 agataacaat aaagttgttt atgatgaaaa ctttaaatac aaagaattta atagtcatat
    18901 gtcgcttgat ttacctacag aatatttaac aaagaatgtc aatggccatt caaaaatgta
    18961 ggtttttggc catttagaaa tgtcgttttt tggccaccaa aaatgtaggt ttttggccca
    19021 taaataacaa ggttacttat acgacaactt tttttcttta taatctttaa ttcgataaga
    19081 ctttccagta atttgaataa tcttagcgtg gtgcactaat cgatcgatta gtgcatttgt
    19141 taatttttta tcttgaaaag tttcagccca ttgagacaaa ggaatattcg ttgtaataat
    19201 ggttgatttt ttttcatagc gtagattaat taattgaaag aataactttg ccccgtcagg
    19261 ggtaaaaggc aaataaccaa tttcatcaat aattagtaaa tcgtaagaag catatttttt
    19321 taacaatcga tctaaatttc cacgttgatt tgcacgattt agtcgattga ctaattcatt
    19381 gcttaaagca aaatggcaag aataaccttt ggttatagct tctagtgtaa tggcaatagc
    19441 taggtgagtt ttccccactc cgctatttcc aataaacaat atattattat ttgtatccat
    19501 aaagcgtaag gtacacaaat ctaaaatctc tgttcgatta atctttggtt gaaatccaaa
    19561 atcaaagtca ttgattgttt ttttaaacgg taaattcgcc tttcttaaac gattttcttt
    19621 ttgtcgttca tttcgtgagt ttatttcttc cgtcattaaa tgatttaaag cttctacaaa
    19681 actaagattt tcttgaacaa tggtatcgag atattctggt aaaatttctc tcgtatgttt
    19741 taaattcaaa tcttctaatt tgtttagtaa atgataataa ttagacataa tgattcacct
    19801 ctctataatt cgtcatacgc ttgtaaattt tcatcaacaa aacgttctaa ttcttcgtct
    19861 tctaaatgtt taaaaacgtc agaacgtaga atttctatat agtcatttcg atgataatta
    19921 agcaattgat gagtcatcgt atgtgtgcga ataagagtag agtcatcgta aatataaagt
    19981 ttatcttctt ttacacgaag tgtaagaatt ttaccgatgt aatttactgg cacagaatat
    20041 ttattttgat tccattgaac catcgcttcg ttagatactt ttcgtttaat ttgtttttca
    20101 acagaagcat aaattttaag ttgattcgca tcaaaagaag atagttgttc tttctcgtat
    20161 gaccaacggt cactaggaca ttcatgaatt gcttgagaaa tttcttcagt atttaatgaa
    20221 tccatgattg tttgaataat tttttctaat tcgtgagtag attcaaattc ataattaaat
    20281 acacgaattc gttcaattgt tcgtgcaaga gcttcaactt ttccttttgt ttgaggtcga
    20341 aaagcacgac aagcaattgg aagaaaacct gcttctttcg cgtattgtcg gaaccgctca
    20401 ttaaaaataa ctttagaaaa ttgactacga ctacggtcaa caacagtttt catattatca
    20461 aaccatattt ctgtaggaac acctccggtg tattgaaaag cattagttaa gcaacgaaat
    20521 aaagtagttt gagaacgatc aaaagttagt tctaaatact taattctgga atatcctaaa
    20581 acgtaaagga agatattaca agaaataact tcaccgaatt tattgactaa tttgaggtct
    20641 tctttccaat caacttgagc agataagcca ggagttgttt caacacgaat cgttgctttt
    20701 tgtattttat ctgctttgat attgcgacaa tatcttctca gtgttcgtgc acttccacga
    20761 tagccacgtt tagaaataaa atgataaata gcagaagaag aacaacctaa agctaatttg
    20821 tcagcgataa tatcttcaaa atcactaacc aaagtttttt tgtttttatt cttttgagca
    20881 agtaaaggta atttattgct tattccagct tcataataac gttttactgt acgataatcg
    20941 caattgtatt gtttagctag agcagcataa ttaggttttg tatgagtcat aatgaaaggt
    21001 aacactcctt cataaatatc ttttctcaat acactttcct ccttgaataa aataacacaa
    21061 ggaaagcgta ccagatgacg cataaaccta catttttatt tggccatttt tctacatttt
    21121 aacttggcca tttacaacaa agaataaaaa aatcaattat agtgttgata ttcttttagt
    21181 ggataatcca gataaaaaca agttcgaaac tgaaactaaa gatttaagaa cgatcggtta
    21241 tacttcttct gaaaaagttt taacaaacaa agatatgaca aataaccaag tcaactattc
    21301 agcagtagtt agaacagtga aagaaagaga gaataataat ccggtcaaag agttcaatga
    21361 gagtatcaat tataaattta ctgatagcac taaaacaaaa acaggatatg gtatatcagt
    21421 tgatttagag ccaacatata gaaatgaaat tgggaaacaa atagatatga aagtctatag
    21481 ttatccgatg aatgaattga ttgacagcaa aattgacgaa atctatcaaa gtgggggaaa
    21541 agaaacagaa gttaagatgg atcgcttaaa gaatgatggt aaagttgagt cgggtattag
    21601 tactcaaaat ctttcattcc aatttccaca tatgaatatc gaaagaaaaa ctggaaatat
    21661 atatgctgac agtcaaagaa atgatgaatc tattaagtat gaattgatcg atggcggtag
    21721 aaaattatat gtgcctattt ggtgtgattt gggaaattat tctattgcta tcaaaagtag
    21781 taactttggt ggtaactata tctcgttgga tatgaaccag aacatagatg tttatgctta
    21841 tatgtatgca acgatagata gtgaaacaaa aaaagatgat gaattactgc ttgaacctgt
    21901 ttatccagag tctactctac cagatggttg gagcaaaaat gagattgatt ggctgaataa
    21961 ataagtttga aaagttttta tctatctgat agaattaaat aatgatatag ggatggtgat
    22021 agaatgaggg ttcaaagcta tggtaacagt gtctttgatg ttaaaagaga atttcaagat
    22081 tctagaagaa aaaccaaaaa gctgatagct tacttcattc tatcagctat cgttttctat
    22141 attgtcaagt ttgttttgga attaaactca atagtattca cagatatggt tcataaaatg
    22201 ttaaatggag aacctattaa tttcaacttt gattacatgc ttgaaaaagc acaaagcaat
    22261 caaattgtgg cttttatcgt gagtatctat tattttattg aaaaatctat tttttcttta
    22321 aaagattttt tctttgaaat gcttaccaaa ctaacaacgt tttacaataa aatttcaaaa
    22381 aattaaggag gaagtttgaa tgttagatca aaatacagac agaagttggt ggatgattgg
    22441 tgccgtgatc gtaggtgcca tcttaatcgg gacagctaaa gtagcttttc ccgaagtgtt
    22501 taatatcgtc attacatttt tcaaaaacat gatccctaca aacttaggat agttccacta
    22561 aaaaattcac gtagtttgat gcgtagaaaa ataataaaga atgaaaaagg ctacataaag
    22621 tgatttatgt agcctttttt ttggatggaa gtgatgtaat gctaagagat atgcccaaaa
    22681 tgattctttt aatgtttgtt gggttctttt taattttagt gattgcgtac tattcattaa
    22741 atggctatac acaagattcg tctgtgaaag gtatgcaaga aacgttaaga actacagcaa
    22801 tagcaaacag ggatgatagt attcgagtag aaaaaggacg atttgcttta attaagtctg
    22861 attttgaaaa agattttaaa gaaaaatttg ggaaaagtaa aaatctaaat aatgaaaccg
    22921 ctgattataa atttgattat cttacagatg ggaaggacgg tattaaagca attaaagtga
    22981 aggttgaaag tggatcaaaa gaatatcaag caacttgtat tttaaatgtt gcaaatgatt
    23041 agaggtgtga aatttgcgtg ttgtagagga aaagaagatt tctccaaaaa tcgaggaaac
    23101 tcctaaaagt aaaattaata tcgcaagtat tattaaaaaa aatggaatta aagaaaactc
    23161 tgaagcgata gaaggaaacc ttgatttttt aactaagctt ttagaagaag atagtggaaa
    23221 ttatgaacca ccaattgaag atgcaaaatt aaatatgatt cgtgatgatt taaccagaaa
    23281 ctattcccaa gaagttttac aagctttaac tgatgatgaa attaggcgaa aacttgtaca
    23341 agttgttttg aaagaacatg gtactttctt taaaaaagat aaagaggttg cagaatatgt
    23401 agtacgtgaa tgtattggaa cagggattat tgagcgaatt atgaccaacg atgaaattac
    23461 agatgttggt tggaatggct ctcaacttag cattgaaact aactctaaca aatggttgtt
    23521 tgacggtgat aaattaaata tcacgcaaga gtatattgaa cgtgttattt ctaaatatgc
    23581 tgttgtaaat gatcgggctt ttaatagtgg taatcctatc atggatggaa tgtttaaaaa
    23641 tgttcgtata tcagctacac acatagataa ttcaccagat ggctctacgt tttctatgcg
    23701 tgtggcaagg cctagattag cattgaataa agaaaacttt gaagattttg ctcctgatta
    23761 tattttggag ctatttgaga aaatgatgaa aacaaaagca aatatctgta tatcagggga
    23821 gacaggtaca ggtaaaacgg aacttcaaaa attactgcta agttttattg ataatcatga
    23881 tcgtatgatt atgattgagg acgttcaaga aactcacgct aaagaattat ttccggataa
    23941 agatattttc tcttggatca ctactcccaa aaaagcaatt tcagatttag taaaagcttc
    24001 tcttagaaat aatccacgat ggatcattgt ttcagaaaca cgtggttctg aagcttatga
    24061 aatgcttcaa gcggttttgt caggtcacta tgttgtaacg acacttcatg cggttaatgc
    24121 acgtgcgata cctagacgtt ttgtaaatat gtgtgcaagt ggttttaaaa taaacgaaaa
    24181 tgcggttgag gaagacataa atcgttattt cgattttggt gtccatatca aaaaaacagt
    24241 ggttgatggt aaagtgatcc gttatttgaa tgaaattgtt gaattttctg ctagtgaacc
    24301 gactacagtt tttaagcaaa aatttatcaa tggaaagttt gtcattgaaa atggtagtct
    24361 ttccgaagat tttaaagaaa aaatggctga aaaatttatt acgtttgaat ttcctgaaga
    24421 tcaaactaat acagaagaat cacttcctga agaagaagag gtgatttaag ttgaaagtac
    24481 aggctatgac aaagcgaaaa ggtctcatgg atgtttcgga actaaacgcc tatcaagtag
    24541 cgtatggtaa accgttagat tatagagatt atttgaacta tgttggatta ccttgtttgt
    24601 ttatgggtgg cttttctttt gctttatgtt actactggtg gattgctttg atttgtggag
    24661 taattggagg cgtatatggc tttaaagtta taatgcctaa aagtattaaa cgctcttatg
    24721 aattaaagag tttacaggaa agaaataaat ttattaacaa catgactcaa attttgaccg
    24781 atgatagtaa aacagttact agagcgttat ctatagcaaa acagcgaaca aaaggtgaat
    24841 taagagaaga tattcaaatc ttagaatcac gtttacaagg tgctgataaa tttcagattg
    24901 ctgaagcttt tcgtgaaatc aacaataaat attatagtga tgttgttttt acacaatact
    24961 ttgagcaatt ggaaacagct atttacgaag gtagaaataa ttcagatact atgaagcaaa
    25021 taaagagcta tcataatgat gtgaaacgta aaacagaagg ttttatgaaa atcaaagacg
    25081 gctatcttca agatatgaag caaatgatgt ttattgtagc tgtttttatt ggagctataa
    25141 cttttgcttt tggtttttct actttctatg aaggttttgc tcgatcacct atcggatggg
    25201 tatttggatc tatttacttt ttgattatgg ttagcttcat gaagtcattt ttcaaacatt
    25261 attttgatga tgaaataatg agtttggggg taagtaatta atgaaagtta aggctttaaa
    25321 aaagaaaaga aaatcaagta ttagcccctt gaaaattacg aaaacagaac ataaaagctt
    25381 ttggattaaa ttactaggtg atgtttatta tcaaaaatta cgtgaaatga acaattccaa
    25441 tattgcgatt attaagtggc aaaaacaacg gttaaaaaag tcattgattt ttatcctagt
    25501 cggattctta atgtatctat ttttagggaa agaatggtat tacttagccg gtggaattgc
    25561 tacttcagta ggtttttatg tttggcaagc aaaatcaatc aatggtatct ataatcaatt
    25621 tcggtttgaa cgacacttgc aatttagtaa atttacacgg ctattgatcc cctatctgaa
    25681 acaaaaaaaa gatggcggaa atttatatgg tgtttttgca aaaatcgtaa aaagactaga
    25741 ttatgatacc gataaaaagc tattaatgaa gcttatgcaa gaaatgacag atcagcctaa
    25801 cgatattaga ccttttacgg aatatgcaga acagacttct ggaacagaca tgtccgtatt
    25861 atttatgtct acagtttatg atattcgaca aggttcggca gatttggatg ttattgaaga
    25921 gttggatcgt ttagcttctg aagagttaat ggccggaatc gattcaatca ttgactataa
    25981 gtcgaggcgt tttgtcttct ttcctacaaa aattgcaatg acttcattta ttttagttgt
    26041 cggttttgca gtaggcgttc ttttcatgaa cctaaaagat attaactttg ggcaataaag
    26101 gaaaggagaa agtcaatgca agaacaatcc gttatggaaa tcattaaatg gtttataggt
    26161 ttaatgatga ttatggcaat ggtttctata ggactttttt gtgttcaatt acaagatatt
    26221 aatactttca agcaacaagt aaattatcaa attgaacgaa aaggaggtct gacgacagaa
    26281 gctgttgaag caatcgacaa atattcggct actaattatg atagacgttt tacaattgaa
    26341 agcgataagt taaatcagaa agtatcttat ggagaagaaa ttgattatac gatcaaagca
    26401 actattccaa ttcaaatttt accgcttcct gatgtaactt tatcgtttaa cggaaatagt
    26461 gtttctcaaa taagatgaga ggaaggataa aatgcaagag aataatgttt ccagaataat
    26521 ttatttgatt ggcggattga taattttatc cttagctatt gccggtcttg tagagcattg
    26581 gccggaaata aagttattaa taagaaactt ttacgacaat aaaatgatta aaatgtagga
    26641 ggtatagatt tggaagcaat tcaagaaaat cgtaaggata atcaagaaac aagtgaaaaa
    26701 aacaaaaaaa aaagaaaaaa gaaagtagat aaatttacgg aaatgaccga taaaatcatt
    26761 gaaaagaaag gacttaaacc taaagacttt gaccgtcaag caatcaaaga agcacgacaa
    26821 aaactgtatg atgattttgt taaagaaaac atatcacagc ttttagacta ac
//
LOCUS       DACEOE010000020        26097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEOE010000020 DACEOE010000000
VERSION     DACEOE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.021"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(130..825)
                     /locus_tag="IW774_000735"
     CDS             complement(130..825)
                     /locus_tag="IW774_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1153104.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IW774_000736"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IW774_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1153105.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="IW774_000737"
     CDS             complement(2024..2215)
                     /locus_tag="IW774_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1153106.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="IW774_000738"
     CDS             complement(2236..3126)
                     /locus_tag="IW774_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1153107.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="IW774_000739"
     CDS             complement(3113..3880)
                     /locus_tag="IW774_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1153108.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IW774_000740"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IW774_000740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1153109.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IW774_000741"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IW774_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1153110.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IW774_000742"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IW774_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1153111.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="IW774_000743"
     CDS             8496..8834
                     /locus_tag="IW774_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153112.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="IW774_000744"
     CDS             complement(9002..9760)
                     /locus_tag="IW774_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1153113.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="IW774_000745"
     CDS             complement(9778..10596)
                     /locus_tag="IW774_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1153114.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IW774_000746"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IW774_000746"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1153115.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IW774_000747"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IW774_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1153116.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="IW774_000748"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="IW774_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1153117.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13256..14386
                     /gene="dnaN"
                     /locus_tag="IW774_000749"
     CDS             13256..14386
                     /gene="dnaN"
                     /locus_tag="IW774_000749"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1153118.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14615..14857
                     /gene="yaaA"
                     /locus_tag="IW774_000750"
     CDS             14615..14857
                     /gene="yaaA"
                     /locus_tag="IW774_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1153119.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14844..15968
                     /gene="recF"
                     /locus_tag="IW774_000751"
     CDS             14844..15968
                     /gene="recF"
                     /locus_tag="IW774_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1153120.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15965..17911
                     /gene="gyrB"
                     /locus_tag="IW774_000752"
     CDS             15965..17911
                     /gene="gyrB"
                     /locus_tag="IW774_000752"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1153121.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17931..20402
                     /gene="gyrA"
                     /locus_tag="IW774_000753"
     CDS             17931..20402
                     /gene="gyrA"
                     /locus_tag="IW774_000753"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1153122.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20638..20937
                     /locus_tag="IW774_000754"
     CDS             20638..20937
                     /locus_tag="IW774_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1153123.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20987..21499
                     /gene="ssb"
                     /locus_tag="IW774_000755"
     CDS             20987..21499
                     /gene="ssb"
                     /locus_tag="IW774_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1153124.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21525..21761
                     /gene="rpsR"
                     /locus_tag="IW774_000756"
     CDS             21525..21761
                     /gene="rpsR"
                     /locus_tag="IW774_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1153125.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21904..23877
                     /locus_tag="IW774_000757"
     CDS             21904..23877
                     /locus_tag="IW774_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1153126.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23883..24335
                     /gene="rplI"
                     /locus_tag="IW774_000758"
     CDS             23883..24335
                     /gene="rplI"
                     /locus_tag="IW774_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1153127.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24609..25976
                     /gene="dnaB"
                     /locus_tag="IW774_000759"
     CDS             24609..25976
                     /gene="dnaB"
                     /locus_tag="IW774_000759"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1153128.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         7921 a         5135 c         5178 g         7863 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact
    13201 tttattattc tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa
    13261 agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc
    13321 aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt
    13381 gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga
    13441 aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag
    13501 cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca
    13561 agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta
    13621 tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac
    13681 taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac
    13741 aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg attctcaccg
    13801 tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc
    13861 tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat
    13921 cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt
    13981 gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt
    14041 tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga
    14101 aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa
    14161 ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc
    14221 attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat
    14281 gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga
    14341 tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat
    14401 gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta
    14461 aaatctcttt taaggctttc tgtttatttt tgtttaaatc acctttttta gattaaatcg
    14521 tcttatatgg aaaaatatat ggataaattt gttttattgc gaaaaaaaag gtataataga
    14581 gtatactttt aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac
    14641 ggaattcatg acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca
    14701 agcaaaatgg tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg
    14761 tggacgcaaa ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat
    14821 ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa
    14881 attacgaaga gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg
    14941 cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc
    15001 ggacaagtag tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag
    15061 agatcacaaa aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa
    15121 aaaccaagat caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg
    15181 tcattttatt tgcaccagaa gatctttctt tagtaaaagg cagcccgcag accaggcgga
    15241 agtttttaga tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat
    15301 accaagcaat tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa
    15361 ctgatttgct ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat
    15421 tgaaagcaag agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc
    15481 aaatcaccca tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag
    15541 aaacacatac gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa
    15601 agaaagacct ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt
    15661 ttattaatca gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac
    15721 taagtgtcaa attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc
    15781 ttttgttaga tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa
    15841 caatcgaagg aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata
    15901 aaatgactgt tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa
    15961 ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat
    16021 tcaagtactt gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac
    16081 tagttctgag ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt
    16141 attagcaggt tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt
    16201 tattgacgac ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt
    16261 tgaaacggtc tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt
    16321 ttctggtgga cttcacggag taggatcttc tgttgttaac gcgctttcta cacagcttga
    16381 tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt
    16441 ggacgactta aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc
    16501 agaccctgaa atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat
    16561 ccgtgaactt gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga
    16621 agaaccagtc atcaatgaat accattatga cggcgggatt aaaagttatg tcgaatactt
    16681 gaatgcaaac aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga
    16741 tatcgcagtt gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt
    16801 tgccaacaat atccatacgt atgaaggcgg aacacatgaa tcaggattca agactgcttt
    16861 aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa
    16921 cttgaccggt gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga
    16981 ccctcaattc gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac
    17041 tgatcggtta tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg
    17101 tcaaattgtt gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg
    17161 tgaagtaacg agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga
    17221 ctgctctagc aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg
    17281 cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa
    17341 aatcttgaat gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc
    17401 tttatttact gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta
    17461 ccacaaatta gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct
    17521 gctgactctt ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc
    17581 tcagccacct ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa
    17641 aaatgcagaa gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag
    17701 cgttcagcgt tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat
    17761 ggacccaagc aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca
    17821 gatatttgaa atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa
    17881 tgcacactat gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag
    17941 aaatcagaga aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg
    18001 attatgcgat gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac
    18061 ccgttcatcg ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc
    18121 ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagact
    18181 atgcgatcta tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag
    18241 tggatggtca tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata
    18301 ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg
    18361 ttgatttcca aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct
    18421 tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc
    18481 cacctcataa cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg
    18541 tgacgaccaa tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg
    18601 ttatgggaaa atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac
    18661 gtgcaaaagt tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat
    18721 tgccttatat ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca
    18781 agcgtatcga agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg
    18841 tcattgacat ccgtcgtgac gccagcgcat ctgttatctt gaataactta tataaattga
    18901 catccttgca aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag
    18961 ttttgagctt gaaacaaatt cttgaaaact atatcgaaca tcagcgcgaa gtcatcacaa
    19021 gacggacagt ctttgaaaaa gaaaaagcag aagcaagggc acacatttta gaaggtttgc
    19081 gtattgcttt ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg
    19141 acgaagcaaa agcgacgatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta
    19201 ttttagatat gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat
    19261 accaagaact cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg
    19321 ttacagaaat catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac
    19381 gcacagaatt actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag
    19441 aagaaatcgt tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat
    19501 tcagagctca acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact
    19561 tcgtcaaaaa tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg
    19621 gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata cgggcgcaca gccaaaggaa
    19681 taccaattat caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg
    19741 tgacgggaga agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa
    19801 aacgtacttc tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt
    19861 tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta
    19921 ttggaacaca taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac
    19981 gtacagcttc cggggttaga ggaatacgcc tgcgtgagga agattatgtc gttggggcag
    20041 ccttaatgaa ggaagatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga
    20101 caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca
    20161 tcactgaaaa aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca
    20221 tgctgattac agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg
    20281 gacgttcaac attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga
    20341 tggcagcagt cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat
    20401 agccatcaag actaattgtg taagctagca agaatgaccg attaaaaaat ctggttattc
    20461 ctgctggctt ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta
    20521 ttgcttttga ccaaaataga gtgtataatc agagcttgtg agtagtctaa ttaattagat
    20581 aactctcctt gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg
    20641 gaaaacacga aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa
    20701 actgctttaa tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa
    20761 tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc
    20821 tatcacatcg ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt
    20881 cttgctaaaa tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg
    20941 ttgtttcatg tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt
    21001 tgtattagtt ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc
    21061 agtcgcaaca tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga
    21121 ggctgatttt atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc
    21181 tcgtaaagga acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca
    21241 gcaaggacaa cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag
    21301 tcgttctgca tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc
    21361 tggcggattt ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc
    21421 tgatttcgac cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga
    21481 cgatgattta ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa
    21541 gaggcggacg taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg
    21601 attataaaga tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac
    21661 gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg
    21721 ctcgtatcat gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat
    21781 gactgatcct tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg
    21841 tttagtcgct cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa
    21901 attatggaaa ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata
    21961 ttaatcgaag tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt
    22021 ctattgatga atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta
    22081 aataaacgtg aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc
    22141 tcagaagtca tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg
    22201 ctcaatccat tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta
    22261 gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca
    22321 tttcagtttg atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac
    22381 cagttgttaa aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat
    22441 aactatgcag atgccattga taaattggat gacaaagaga tttcttattt gaactctttc
    22501 gtcactacac tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac
    22561 gcagaacggt ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa
    22621 tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt
    22681 agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat
    22741 aatttagatg ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa
    22801 ggagcaaaac ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt
    22861 cgttctcgag cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt
    22921 atcatggggc acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt
    22981 ctggcacgct tcaaccagaa aaaagcttac atcgttatca acgagaacga gatcattcca
    23041 gatgtagaac ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc
    23101 tctccagaac gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac
    23161 cataaacctt ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc
    23221 attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatatcgaa
    23281 tcttcagctt cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa
    23341 acaaatcgct tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca
    23401 aatagtttca atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct
    23461 tgtggagcag acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg
    23521 gagatcagca atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca
    23581 gggacagacg aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt
    23641 tcgatgatca atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg
    23701 atcagtgcca gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc
    23761 ggaggacact tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa
    23821 cagaagctat ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc
    23881 aaatgaaagt aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag
    23941 aagttccagc aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa
    24001 cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg
    24061 aaatcttgga agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg
    24121 aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa
    24181 ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag
    24241 aaaatccgat ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga
    24301 cggctacgat caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct
    24361 ggtaagtata accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag
    24421 caatgaaagc aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca
    24481 gcaaaatagc ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc
    24541 ggtacgaatg atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag
    24601 cgatacgtat gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac
    24661 aagcggtttt aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca
    24721 tagagccaaa agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt
    24781 taaatgatcg taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca
    24841 atttgttaga agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga
    24901 cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat
    24961 tgatccagac agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa
    25021 caattttaga tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg
    25081 gctttttatc tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc
    25141 aaaacaatga agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag
    25201 caggtttcca gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag
    25261 cgtttgcatt gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat
    25321 ttagtttgga aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga
    25381 ttgaggccag ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg
    25441 ttgcgatggg gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa
    25501 tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga
    25561 tattgatcga ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag
    25621 tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg
    25681 ctctttcaca gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg
    25741 atattcgtga atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag
    25801 atgattatta tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca
    25861 acgtcatcga agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac
    25921 tgtttatcaa agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa
    25981 gaggttgtga gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc
    26041 aggaacgact agagtactta atttaaacag atctccaaag ggattgtgac aaaagtc
//
LOCUS       DACEOE010000021        25751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEOE010000021 DACEOE010000000
VERSION     DACEOE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.022"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            9..851
                     /locus_tag="IW774_000760"
     CDS             9..851
                     /locus_tag="IW774_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1153129.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            848..1798
                     /locus_tag="IW774_000761"
     CDS             848..1798
                     /locus_tag="IW774_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1153130.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1813..2046
                     /locus_tag="IW774_000762"
     CDS             1813..2046
                     /locus_tag="IW774_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1153131.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2377..2961
                     /locus_tag="IW774_000763"
     CDS             2377..2961
                     /locus_tag="IW774_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1153132.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3092..3759
                     /locus_tag="IW774_000764"
                     /pseudo
     CDS             3092..3759
                     /locus_tag="IW774_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
     gene            3761..5185
                     /locus_tag="IW774_000765"
     CDS             3761..5185
                     /locus_tag="IW774_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1153133.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            5203..5772
                     /locus_tag="IW774_000766"
     CDS             5203..5772
                     /locus_tag="IW774_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1153134.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5882..7636
                     /locus_tag="IW774_000767"
     CDS             5882..7636
                     /locus_tag="IW774_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153135.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7590..9362
                     /locus_tag="IW774_000768"
     CDS             7590..9362
                     /locus_tag="IW774_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153136.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            9885..10154
                     /gene="rpsN"
                     /locus_tag="IW774_000769"
     CDS             9885..10154
                     /gene="rpsN"
                     /locus_tag="IW774_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1153137.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10169..10348
                     /gene="rpmF"
                     /locus_tag="IW774_000770"
     CDS             10169..10348
                     /gene="rpmF"
                     /locus_tag="IW774_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1153138.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10380..10757
                     /locus_tag="IW774_000771"
     CDS             10380..10757
                     /locus_tag="IW774_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153139.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10778..10927
                     /gene="rpmG"
                     /locus_tag="IW774_000772"
     CDS             10778..10927
                     /gene="rpmG"
                     /locus_tag="IW774_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1153140.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10951..11934
                     /locus_tag="IW774_000773"
     CDS             10951..11934
                     /locus_tag="IW774_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1153141.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11964..12086
                     /locus_tag="IW774_000774"
     CDS             11964..12086
                     /locus_tag="IW774_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1153142.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12104..13627
                     /locus_tag="IW774_000775"
     CDS             12104..13627
                     /locus_tag="IW774_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1153143.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13651..13890
                     /locus_tag="IW774_000776"
     CDS             13651..13890
                     /locus_tag="IW774_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153144.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14083..14544)
                     /locus_tag="IW774_000777"
     CDS             complement(14083..14544)
                     /locus_tag="IW774_000777"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1153145.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14739..14957)
                     /locus_tag="IW774_000778"
     CDS             complement(14739..14957)
                     /locus_tag="IW774_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1153146.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15075..16691
                     /locus_tag="IW774_000779"
     CDS             15075..16691
                     /locus_tag="IW774_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1153147.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16932..18272)
                     /locus_tag="IW774_000780"
                     /pseudo
     CDS             complement(16932..18272)
                     /locus_tag="IW774_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18548..18703)
                     /locus_tag="IW774_000781"
     CDS             complement(18548..18703)
                     /locus_tag="IW774_000781"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1153148.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(18999..19331)
                     /locus_tag="IW774_000782"
     CDS             complement(18999..19331)
                     /locus_tag="IW774_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1153149.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19845..20036)
                     /locus_tag="IW774_000783"
     CDS             complement(19845..20036)
                     /locus_tag="IW774_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1153150.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20130..20516)
                     /locus_tag="IW774_000784"
     CDS             complement(20130..20516)
                     /locus_tag="IW774_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1153151.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21210..22415
                     /locus_tag="IW774_000785"
     CDS             21210..22415
                     /locus_tag="IW774_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1153152.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            22432..22866
                     /locus_tag="IW774_000786"
     CDS             22432..22866
                     /locus_tag="IW774_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153153.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            23483..>25751
                     /gene="esp"
                     /locus_tag="IW774_000787"
     CDS             23483..>25751
                     /gene="esp"
                     /locus_tag="IW774_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1153154.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         8873 a         3681 c         4821 g         8376 t
ORIGIN      
        1 ggaataatat gcttcaaaat tttattaatg gtctgtatga ctttcacttt ttacagaatg
       61 ctctaattac atctgtagtt atagggattg tttctggaac agtaggttgt tttattattt
      121 taagaggaat gtctcttatg ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa
      181 tttcttatat tttaggtatt aattttttta ttggtgcgat tgtatttggt ctattagctt
      241 ctatcattat tacttttata aaaagcaata gtgtaataaa aggagataca gcaattggta
      301 ttactttcag ttctttttta gcattaggag ttatccttat aggagttgca aatagctcaa
      361 cggatttatt tcatatctta tttggaaata ttttagctgt acaagatatt gataagtgga
      421 ttacaattgg cgtttctgtt ttagtgttgg ttacagttgt tctatttttt aaagaattat
      481 taatcacttc ttttgatcca ttaatggcaa aagcaatggg gatgaatgtc agtttttatc
      541 attatctatt aatgattcta ttaactttag tagcagtaac agctatgcaa agtgtgggaa
      601 ctatattaat tgttgcaatg ttaattacac cagctgctac agcatattta tatgcaaaca
      661 gcttaaaaac tatgatattt atttcggcga cagtcggagc gtgctcatca ttgctaggtt
      721 tgttcattgg ttatagtttt aacatagcag caggatcaag tattgtttta acagcggcat
      781 tcttatttgt aattagtttt ctaatctcac caaaacaaaa atttttaaga aagaaggaga
      841 gaaagttatg atgagaaaat ggaaagcagt actgggaagt ctggggattt tgattgctct
      901 ttttatattc ggggcatgtt caacaaatag taaagacaaa gatacagtgg cttcgaacga
      961 aaaattaaag gtagtagtta ctaattcgat tttagcagat attactgaaa atatagcaaa
     1021 agataaaatt gatttacaca gtatcgtacc tattgggaaa gatccccacg aatatgaacc
     1081 tttgcctgaa gatgttcaaa aaacttcaaa agcagatttg attttttata acggtgttaa
     1141 cttggaaact ggaggaaatg cttggtttac aaaattagta aaaaatgcga acaaagagga
     1201 aaacaaagac tattttgcag caagtgatgg catagatgtt atttacttag agggtcagag
     1261 tgagaaaggg aaggaagatc cccatgcttg gttaaattta gaaaacggta ttatttacgc
     1321 taaaaatatt gaaaaacagt tagcggaaaa agatcctgat aataaaaaat tctataaaga
     1381 aaatctagat aagtatattg aaaaattgga ttctctagac aaagaagcta aatctaaatt
     1441 tgcttcaatt ccgaatgata aaaaaatgat tgttacaagt gaaggatgct ttaaatattt
     1501 ctcgaaagcg tataatgtgc cttctgctta catttgggaa atcaacactg aagaagaagg
     1561 aacaccagat caaataaaac acttagttga aaaattacgc acaacaaaag ttccctcctt
     1621 attcgtagaa agcagtgtgg acgatagacc aatgaaaaca gtatcaaaag ataccaatat
     1681 tcctatctat tcaacgattt ttactgattc aattgcagaa aaaggacaag atggtgatag
     1741 ttactatgcg atgatgaaat ggaacctgga taaaattgct gaaggccttt cgaaataatt
     1801 tggagggtag gtatggtaca aattaaaacg actactatac taatttattt actagcaatt
     1861 caggaattat caaaaagaaa aaagaaatta aaaaataatg atttagcaaa agttctaagt
     1921 gttagtccag catctgtaag tgagatgctg gctaaactta gatatgagga ctatttagat
     1981 ataggatttc aattaactga taaagggaaa aaatttattg acgattacaa aaaaggtttt
     2041 aagtaaaaga ctttattttt agggaggata agaaaatggc tattggagtg attatgatag
     2101 gacttaccct aatagctgga tataattttt ttaaatcttt ctcggattat tttagaaaag
     2161 gtgaatgatt tagatgagtc tattaattct tgtaggattg ctttttgtat cagttatttt
     2221 gctctttctc attagtttag ctctaaggaa tgagttcagt aatagacgtt aacgattgaa
     2281 acttcaagtc ctgtgtaaga taatatagtt aattcatctt attaggagga agtacccata
     2341 gaaaccagag attatattac aactcaccaa aaattaatag aaagtggact aagccatttt
     2401 gaaaaagaag gatttcagca aactaagata aggcaactgt gcttgaccgc aggcgtaact
     2461 acgggtgctt tttataaaca ttttacttct aaggaagcgt tatttgaaga aattattcag
     2521 ccgcatatta atgcgcttat taatatgtat aatgaagagt ttaccaaata cctcttaaac
     2581 ttagaaaata tagatataga gaaaagttgg agagagaact tcaatgaatt agatccattt
     2641 attaattata tttttgagaa taaacgtata ttcgatttgg ttttattcca atcagctgga
     2701 accaaatatc aagatattgt tgaaaaaatt acatcatttg tgacaaaaaa gacagtctgt
     2761 tgtctagata agttaaagac tatcggaatg atcaatcaaa atttagtctt taatgaagaa
     2821 gaaatccact tctacattta ctcattctac gctacattct atgatatatt gaagcatagc
     2881 tattcaaaag aaaagactat gctattaacc agaagattgt atcaattttc tgttcctggg
     2941 tggattgaat tactgagtta attaaaaaaa agcttgcatt gagaattgtt ctcagttatg
     3001 ctttttgata agatcgcact gttatctgaa taacggtgca tttttgtcag cttaagataa
     3061 cagtgcgatc ttattttttg gaggtgaaag agtgtcaaaa atcaatccgt taaccttgat
     3121 tactttaaat atttttttcc ggtcattatt tttttaggaa agggaaatat atttcaagtg
     3181 gggtgtttca ttattgctac agttttagta ttgatgtttc gtaaaataaa gacatgtatt
     3241 tatttcatct tgatttattc agttctgttt ttagtaatga aaattattga tgctatcaag
     3301 atagagtttt ttactatatt ttttgggaca tccttatata ttgtatttcg aatgttgccg
     3361 gtcgtaatga tctcatggtt gttggttagt gtctatacta gcaatgaatt attatcctct
     3421 ttagaaaaaa tacatttacc gaaaaaaata atgttaagta ttacagtagt gatcagattc
     3481 tttccgactt atcgttctga aataagaatg attaaagaat ctttaaaaat gagaaacatt
     3541 cgcttgtctt tttttcagcc tctgcaatat ttggaatttc tgttagttcc cgttcttata
     3601 cgtgcaacta tgattgctga agaaatgacc gctaatgcaa taacaaaggg gatagaatct
     3661 cctgtgaaaa gaagttctta ctataaaaca aacatgagct tattagacta tatcatcctg
     3721 ttttccgtta ctattttttt cttaagtatt ttgatttgag gtgaaagaat tgtttgaatt
     3781 taacaatgtt tctttttcat ataaggatgt taaggaacaa gcgcttgttg accttaattt
     3841 ttcaataaaa aaaggcagtc ttacattgat tactggttct tctgggagtg ggaaatccac
     3901 actattaaag ttaatgaatg gacttattcc aagactttat gacggaaaat tggttgggga
     3961 aattttattt gaaaataact cgctacaatc cttaacacaa gaaagtatat caaaaaatat
     4021 tggctacgta agtcaaaatc ctagaggaca attctttaca acaaatacaa cgagtgagtt
     4081 agtattttcg atggaaaact ttggatttac aaaagagcat atggataaaa gaatcaatga
     4141 attagctgaa ttattagatc tttcaaatat attagataaa aacatttttt cgctatcaag
     4201 tggtgaacgg cagaaaattt ctattggatg tagtttatcg ctaaactcta aattaattat
     4261 attggatgaa ccgtcttcaa atttggactt caaagcgact aagaaacttg ctgacttact
     4321 ttgtgattta aaaaataaag gatttactat tgttatagct gaacatcgtt tttactattt
     4381 gaaagaatta attgatcaag ttctatttgt taaaaataga actgtaaaaa ctacaactat
     4441 tcagttgtta aaaactgaaa acaataacgc attaaggaaa tttgacattt ttaatagttc
     4501 gattgaagtt ccctatatcg aaaaggtaag tgcagatgta ttaaaagttg aagaaattac
     4561 ttatgaaaat attctaaaag acataaattt tgatgtcaaa gttggagatg ttattggttt
     4621 ggtagggaaa aacggtgtgg ggaaaacaac cttactaaga atactcatga agattattcg
     4681 ccctaccaaa ggaaagataa tcgaaaataa aaaaaatttg tcatcgccgt ttttagtaat
     4741 gcaagaaatg gattaccaat ttttcacaga atcagtgagg agcgagttag ccctggggaa
     4801 tgaaatagtt agtaaagatc agcaagaaaa aattttaaaa aaaatggaac tttataaaat
     4861 gaaagatcaa ttaccgtttg atttatccgg tggagaaaaa caaagattat tggtttctat
     4921 agcttcattg tcaaaaacta atttatttct atttgatgaa cctacaagtg ggttggatta
     4981 tatcaatatg gaaaggattg ctgaccttat taaagactta aaagaaaagg gtgcagtagt
     5041 tatagccact catgatccgg aatttcttta taaaacatgc aatagaatta tttatttaaa
     5101 aaatggtgga ataaaaaaag atataaaact tagagtagaa gattcaactt tgatagaaca
     5161 gatttttaat gacataacga aatagaattg aggagaatat atatgaaaac aaaagattat
     5221 atttttattg gtttagctac agttatttcc ctagtaattt actttttttc aataatggtt
     5281 agtagtattg gtggggcgtt tggtcacagc atatccccag ggatttttgg attattatct
     5341 ggaatcatat ttgtttatat tagctataac taccctagaa aaggaatttt tactatttac
     5401 acaattgttt tattgttatt ttttacgtta atgggcgggg cgtacttacc ttggtttatc
     5461 agttcgatta gtatggcgat cttagctgat attattctat ctgtatttgg atatgatcga
     5521 gcaattcctc aagttgtgtc ttgggttcta atgcaactag gtagtgctgc cggacagtgg
     5581 attcccatat ggttctttac agatcgtttt cgtcaagact ggatagatag aggacaatct
     5641 gctgcaacaa tggatgcaat gatccactac gcggtgggta tatggggaat tatttcagtg
     5701 ttagttgtag caagtttatc tatgattggt gtattgattg gaagaaaagt cttaaaaaaa
     5761 tacaaaaaat agaacgtagt ttaccttcta tctgtttgta catctctaat ttacgctatt
     5821 acggaaagca ttaacagttg taaacgaata agagaagaaa ttgaatgcgg gggaggaaaa
     5881 aatgaaagta tacaaaaaat tatttagtta tgtccctaaa atgaaatact acggatggtt
     5941 agccacttta ttttctgatt tttctgtcgt attaacagtc tatggatatt acagtattta
     6001 caaatttctt cagtctctaa ttattgtagg agatgaatat ttagctaaga gttatgcggt
     6061 tcaaactgtt gcttggttaa ctttaggagc tttattttat attttttctg gcttattttc
     6121 tcatatcctt gggtttcgct tagagactaa tttgagaaaa aagggaatta gcggtttaac
     6181 agaagcaagt tttcgttttt ttgatttaaa ttcttcggga tatattcgaa agacaattga
     6241 tgacaacgcg gctaaaacac atatggcaat tgctcacttg attccagata gtgctcaagc
     6301 catagtaaca cctgttttat cagttgcctt aggctttttc attaacttta aaattggatt
     6361 ggttctaatc gccatgttag taattggtgt attcttgtta aagaaaatga cagagaatac
     6421 aaattttatt caaaaatatc aagaatcgtt agatgtgtta agctctgaaa cggtagaata
     6481 tatccgcgga attcaagtta taaagatatt tggtgctaaa gtaacatcat ttaaagcctt
     6541 gaataaagca attaacgatt atgctaagtt tgcatataag tattctttga gtgggaaaac
     6601 accttttgtt ctttttcaat ggctattttt tggcatagtt tgcattttga tagttcctat
     6661 aacctttttt ataacttctc ttaccactcc caagatctta gctgttgact tgttgatgtt
     6721 gtttttttta agtggaatta tttttgtttc tttcatgaga ataatgtatg taagtatgca
     6781 cttgtttgaa gcaagatatg cagtcgaaac actagactcc ctttatttag aaatgaagca
     6841 agaccaacta aaatatggga acgagacaca gattaaaaat tgtgacattg catttgaaaa
     6901 tgtcagcttt tcatacaaag aagagtgtgt tttagaaaat ttcaacttag ttttagaaca
     6961 aaaaagaatt tatgcattag taggaaaatc aggaagcgga aagagtacga tagctaaact
     7021 aatttcaggt ttctacaatg tggataaggg acagattaaa attggtggta agcctttaaa
     7081 tcactattca aaagacacaa ttattaacac aattgctttt gtctttcaag atacgaaact
     7141 attcaatcag agtatttacg ataacgtttc tatagcaaat cctcaagcgc cagcaagtaa
     7201 agtatttgac gcgctaagat tagccggttg cgaatcgatt ttagaaaaat ttcccgaaag
     7261 agagcatacc ttaattggta gtaaaggagt ttatctgtca ggaggagaaa aacaacgtat
     7321 agctattgcc agagcaattt taaaagatgc aaagattgtt attatggatg aagccagtgc
     7381 atctattgat ccagataatg agtatgaact acaaaaggct tttcaatatt tgatgaaaga
     7441 aaaaacagtt attatgatag ctcatcgatt atccagcatt aaatctgtag atgaaattct
     7501 tgtcttgtca gaaggaaaaa tagtagaacg tggaaatcat aatgaattaa tggctaaaca
     7561 tcgagtgtat aaacaattgg tcactaatta tgagaatgca aatgattgga ggcttgacaa
     7621 tgaaaaattt ttataaaaaa aaatttgctt taactgatca gggcgcagaa gctttaacta
     7681 aagcttctat ttctagtttt tttgtatact gcattaatat ggtaccagca ttcattataa
     7741 tgatgctgat agacgaatta gttttagaaa atgcaaagcc ccgttggctt tattttgcag
     7801 tatcgtttgt tactttgctt ttcatgtatt ggttgttaga tagggagtat gaaaacttat
     7861 ataatagtac ttataaagaa agtgcgcatt taagagtgca aattgcagac gatttgtcaa
     7921 atttaccatt atcctatttt tcaaaacata atttatcaga tttatctcaa actatcatgt
     7981 ctgacgttga aggtattgag catgcgatga gtcatgcaat acctaaagcc ggcggtatgg
     8041 ctctgttttt cccttttatt tcagtgatgc ttttggttgg taatgtcaaa atgggattag
     8101 ctgttatttt gccaacgtta tttagttttg tcttaatctt gttatcaaag aaatcccaaa
     8161 cgaaagccaa tactaaatat tacgatactt tgagagaaaa ctcggaagaa tttcaagaaa
     8221 ctattgaatt gcagcaagag attaatagct ttaatctatc taaaaaagtt caagacagac
     8281 ttttcaaaaa aatggaagag agtgaaagga ttcatttaaa ggtagaatta agtacttttt
     8341 cagtcatggc cttatcctct attttctcat atgttagttt agcagtagtc attctagtag
     8401 gtgttcactt actgttaacg ggtgaagtga ctatactcta cgtcgttggt tacttactag
     8461 ccgcaataaa aataaaggat tccttcgatt ctatgaaaga agcagtgctt gaaatatttt
     8521 atttagctcc taaaatacag cgaattagag ctatgaagga aaccagtatc caagagggat
     8581 cagatagtcc tctgaaatct tttgacattg agttgcgaga tgtctctttc tcatatgata
     8641 acaatacccc aattttagat catatctctt ttacggctaa acaaggagaa gtgactgcgt
     8701 tagtaggtgc tagtggttcc gggaaaacta gtatcttgaa attagtgtct agactgtatg
     8761 attatgatga aggctgtatt ttaattgatg ggtatgatat taaacgtgta tctccagctt
     8821 ctttgtttag caaaatcgct attgtttttc aagaggtaac tttgtttaat acaagtattt
     8881 tggaaaatat acgtattgga aatagtcaag cgtcagatga ggaagtgaaa aaagcagctc
     8941 gtttagctaa ctgcgaagac tttattgaaa aactacctga cggttatcat acattggtcg
     9001 gagaaaacgg aagttcactt tctggaggag agcgacaacg cctatccata gcacgggctt
     9061 ttttgaaaaa tgcgccaatt ttaattttag atgaaattac tgccagttta gatgcggaaa
     9121 atgaaaaacg tattcaggaa agtttaaatc gtttaataca agataagaca gtactaatta
     9181 tatcgcatcg cttgaagtct attgaaaagg taaataaaat tgtagtgatg gatcaaggaa
     9241 aactagttga tcaaggtact cataatgagt tgtatcgaag gtcagaaata tacaaaaatc
     9301 tcataaagaa aactaaacta tctgagaaat tcgtctatga aaaagaagct caatcacgat
     9361 gatgaaatag gctttaaatt aacctacaat ttttgggata tagttataat tctttttcaa
     9421 aaagaataat tttattaaac agcatctaat tttgaataca cgaaagaatc agtatgattg
     9481 aattagttca cttaatgtct attcttataa tataaggaat aatcaaagtt ttaaaaaatt
     9541 ggcgataaac cagttaaaca taaaagattg aataaaaagt tatagtttct ttcatattgc
     9601 cgttttaagt agaggaaaag ctacttaaaa cggtatttgt gtttgattgg actaataaaa
     9661 gtgaattctt atttttcaga gcctaaactg catattcttt taaatgattt gattatctct
     9721 atgtaccgat aaattcgttt tcaaatagtt atctattcat atgagtttta tttcatgtta
     9781 tttattgttg acaagagcaa ctcttgttgg taaagtatca gtgtcaaaac gtaacgatta
     9841 cgatttgagg tgtctaaatc aaaataaaag gagtataaag gtatatggca aaaaagtcga
     9901 aaattgctaa agcaaagaaa caaatggcga tgattgaaaa gtatgcagat aaacgtcagg
     9961 aattaaaagc cgcaggagat cgcaccgctc tcgctaaatt gccaagagat tcaaatccga
    10021 atcgtctgag gcttcgtgat cagacggatg gtcgtccaag agggtatatg cgaaagttcg
    10081 gtatgtcccg tattaagttt agagaacttg cccatcaagg gttgattcct ggagtaaaaa
    10141 aagcaagttg gtaaaaggag gaaatatcat ggcagtacca gcaagaaaaa catctaaggc
    10201 gaagaaacgg ttaagaagaa ctcatcaaac tgtagtaaaa ccagaaattt catttgacga
    10261 agcgaatggt gattatagac gtagccatca tgtatcttta aaagggtact ataatggaaa
    10321 gaaagtaatt aaaggagctt caaaatagtt taatatataa cttttaggag ggaatcaaaa
    10381 tggaaattat ttacccacct ttagttgaag aaggattaaa gtattatctt gaaacgaccc
    10441 agcaatcgct agataaatgc actttttatc gttcgatggt agagagagga attattactg
    10501 aaacgggttt accgacccaa caggcaattg aaaatggatt agtcaaagat tactatgagg
    10561 accaaggctt atcatttgat gattttttaa gaatctatcc aatttttgaa gagtatgacg
    10621 aggagttatt tcaatgtatc gatggttatt gggagatacc tgttgatatg aaagaaaatt
    10681 tagtttcaca attggaatct ggcgagttaa cttttgaaga tgcacaacaa attcaagcat
    10741 atcttgaaga tcgataagta ttgaaggagg aagatttatg cgtcaaaata ttattttgga
    10801 atgtgtcgaa actggagaac gtctgtatct caccagcaaa aataaacgga ataaccctga
    10861 acggttagaa ttaaaaaagt attcgccaaa attgcgcaga cgggcaattt ttaaagaagt
    10921 gaaatagcta agtggtaaaa aggtgacaaa atgggcatac cagttacaat cgtttctggg
    10981 tttttagggg ctggaaaaac aacgctaatc aatcaagcct tgcaagtctc accctacccc
    11041 agggaagaaa ttattataat tgaaaatgag tttggtgaag ttgggattga tcacaaatta
    11101 ttagttcata gcgaggaacg ggtgttacaa ctaaataatg gatgtatgtg ttgcagtctt
    11161 cgcagtgatt tattagcaat attgatttct ttactggaaa caatagaaga acatcaacaa
    11221 ccaatttcac aaatcatcat tgaaactaca ggtatagcag atccccaacc aatcattcaa
    11281 acacttttaa cagcgcctca actaagagga agagtatata tcgatagttt attgactgtt
    11341 attgatagtg acagttttga acttattgaa agagaaccac aagcccttaa gcaacttgtc
    11401 atggcagatc gcatttttgt gtctagaaaa caaaacttga atttatctgc actaaaaaaa
    11461 ttacagagag aaatcaaaag tattaaccct ttatccgata ttcgaatgtt cacacatagt
    11521 gaaacgattc aaaaaaagga tttttataat ttgaaaaaat ttaatcatcc cttagttttt
    11581 gaaagtgatt catcagaaaa tcactataat ggcaaatact atcatcaaca ccatcaagta
    11641 catgagttta aatcaattct cttaacttca gatcaagaca taaaaaaaga ttttttactc
    11701 caatggattg actggctgat tttaaccaac caagggagcc tgtatcgttt taaaggattg
    11761 attgctttac aagagcagga ttttatagtt gccatgcaag aggtaaacga acaggttaat
    11821 tttcaataca ccactcgaca aaaagaccaa accactatta ttctaatcgg aaaaatgttg
    11881 gatgaagaaa ggatcaaaca gagttttgat aaattactgg cggaaagcaa ataatgataa
    11941 gtagtgattg ggggtaattt ctaatggaga aaacggatat atctagtgca tatcgacgac
    12001 ttaaaagccc gaatattaaa actagaaaaa gggcattaaa aattatcaaa gaacataaac
    12061 gaaataaaat gaagaaatta gcataattaa ggaaggaaaa agaatgaaga aaatcagtgt
    12121 aggacttatt gggatagcag ctctaggatt attgggagct tgctccagca ctaatgatgc
    12181 aaaagtatcg aatgataaag atggaaaatt agaaattgta acaacattct atccaatgta
    12241 tgatttcacc aaaaacatcg tcggcgatga agcgaacgtg gatttaatgg ttccagcggg
    12301 atcagaacct catgactatg agccctcagc taaggatatg gcaaaagctc atgatgctga
    12361 tgtttttgta tatcataacg aaaacatgga atcatgggta ccaaaagcta aagaaagttg
    12421 gaagaaagct gggcctaatg tagtagaagg aacaaaagac atgattttat tgccaggtag
    12481 tgaagaagaa gatcatgatc acggagaaga agatcaccat catgaactag atcctcatac
    12541 gtgggtttca cccaaaatgg caattaagga agtttcaaat atcaaggatc agttagttaa
    12601 attgtatccg aaaaaagcaa aagtatttga gacaaatgct gaaaagtatt tgactaaact
    12661 gaaacggttg gatgctgact ataccactag tcttaaagaa gcgaaacaaa aaagttttgt
    12721 gacgcaacac gcagcctttg gatatttagc tttggattat ggattgaaac aagttcctat
    12781 tgctggacta agtcctgaag aagagccatc ttctggtcga ttagctgaat taaaagagta
    12841 tgttaaaaaa aataagatca attatattta ctttgaaaag aatgctaatg ataaaattgc
    12901 aaaaactttg gccaacgaag ctgggataaa attggaagta ctgaatcctc ttgaaagtct
    12961 tacaaaagaa caaatggata atggtgaaga ttatgtttca gtaatggaag acaatctaaa
    13021 agcgttagaa aaaacaacta tggttgctgg tgaagaggta gttccagaaa aagaagcgaa
    13081 ggatgaaaag acagttgcta atgggtactt taaagatgcc gatgtgaagg atcgcgaatt
    13141 gagtgattat acaggcgaat ggcaatctgt ttatccgttg ttaaaagatg gaattttgga
    13201 tgaagttttc gattacaaag caaaaatcaa taaagacatg acagctgccg agtacaaaga
    13261 ttattatact actggctata agacagatat tgatactatc aacattaaag acaacaccat
    13321 tgattttgta gtcaacggtg aacatcatca atacacatac aaatatgttg gatacaaaat
    13381 tttgaattat gaaaaaggaa accgtggtgt tcgttttaat tttgaaactg atgatgcagg
    13441 tgctggtcgt tttaaatata ttcaatttag tgatcatgga attgcaccaa gtaaggcaga
    13501 gcatttccat attttctttg gtggagaaag tcaagaaaaa ctctataatg aattgcataa
    13561 ttggcctact ttctatccag cttcattgtc taaacatgaa attgctcaag aaatgatggc
    13621 tcattaaaat tcaatttttg gaggaaatac atggtaagaa aagtattatt gtctatggat
    13681 tccaagaata ttgatgctta tcggcgttat cgatttgcaa agcaaatgag attaaatggg
    13741 agtacattag ctgatattgc tgcaacttta catgttagta ttgacacagt tcatagagta
    13801 ttaaaacggg atcctgattg ttgttttatc aaggtcaata ctggaagaag tagacgttta
    13861 gcaaaacggc ataatagctt ggccaattaa ttgataatac cagagtagat catttttccc
    13921 ttatttggat ttgatctgct ttttattatg tcttgaattg tcaaatcaag cgcaacactt
    13981 tatcaaagaa gacttcataa ggtgttctcc aatctaaaca ctttctaggt ctgttattca
    14041 gtttttcaat catctgattt atcgttgagt tatctaccgt agctatctct ttcgattttg
    14101 gcaagtattc tcgaatgagt ctattcgtat tttaatttgt tcattgttgc caagggcatg
    14161 aggatctgga aaataaaagg gcatattatt caatgcttct gtaactgcga aatgattgga
    14221 gaattctttt cctctatctg gagtgatcgt tttgaccgcc tcatttggaa tactgcttaa
    14281 aagttcaatc attttctcgc tcacataagc ggctttcttt ctggggacct tctttcctgc
    14341 taaaaggaag catgattttc tgtctgtcaa agtcactaaa caagtaccac cagttttgcc
    14401 agctacaata tcggctttcc aatgaccaaa atcacttcta tcatctgcgg attttggacg
    14461 gccatggatc gaatgagtaa ggcaaatctt tccacgagac tcgacatgat tttttgtatg
    14521 tcttgttttt cctcgatggt gcaatgaacg gatcaggcct ttattgccgt gtgagagggg
    14581 ttctgtatcg aaaagatcat tgcaaattgc acgttagatc gtgataaaac taattgaaaa
    14641 gtccatatgt tcatgcttca aacgattcga gatttcctct ggcgaccact gaaaatcgac
    14701 aaataaatga cgaacaattg accaaactca cggatcattt agaatgtgat aacgtccgca
    14761 tttttttctt cgataagcat aatttttttc cgctttggaa ggtgaatagt ttttttcttg
    14821 tttcttctca gttcatgtaa aatagtaaac gggctatgct gaatgtcgtt gccaattata
    14881 ctaatagaaa atccttcttc gtgaagcaga aagattcttt ctcgttcgag tatagtaaga
    14941 tgacgatact ggctcataat tattttcctt ctaggagtgt tctcgacaaa cttatactag
    15001 ttggatatcg attatgactc atttttatgt gttgcactta aagtgtaaat tcaaggtata
    15061 gaaagaggtt ccctatgaaa aaaataccta ttacaatctt aactggctat cttggttcag
    15121 gaaaaacgat acttctaaat catattctgt acggtgatca cggaaaggaa attgctgtca
    15181 ttgtaaatga cataggggaa gtaaatgttg atggggtatt agtcgacaag aaaggtttta
    15241 aaagaactgg agaaaaactt gtttcgatgc cgaatggatg catttgttgt acattacgcg
    15301 aagatttgat tgttgaacta gatcaattag tgcaattttc tgacttgaat caaattgtta
    15361 ttgaagcttc aggaatcagc aaaccaattc caattattca agcaattata ttagctaaaa
    15421 accctttaga cattgatttg acccaacgat tagagattgg tgccattatt atagtagtgg
    15481 atgtaaaacg tttttgggat aatttcgaaa cgggtgaaaa gattcaggaa agaaaagctg
    15541 aaggtaatgt agttgaagaa gaacaggata ttcgtggctt gttaattgat caattcgagt
    15601 gttgtaacat attactttta aataatagtg acttagtgtc aaccaagacg atttacaaaa
    15661 ttaacggtct aattgctaag ttacagtcca atgccaaaat aattaggaca attaaaggtg
    15721 aagtagactt atcgaaaatt ctaaatacaa atctttttaa tatagatgag acttcctatt
    15781 cagtgggctg gttcaaagag ttagagcttg gctatcaaaa tcatattcct gaaacagaag
    15841 aatatggtat ctcaagtttt atctatcgaa atcggttact ccttgacgca caaaaatttg
    15901 cccatcttac ggataaaaat tttcctggca atattacaag agcaaaaggg tttctttggt
    15961 ttgcccaaca tagtgatttt tcgacgatct ttgaaacatc gggtagctat aaaagtattg
    16021 aatcattaaa ttattgggta gctagtttac ctaaaattga gtgtcaaatg attatacaaa
    16081 aaaatcccga attgaaggaa gcctggcatc cgttgtttgg tgaacgcatt aataaactag
    16141 tttttattgg tatgaagatg gatcaacaat gtattaagga acaacttgag ggttgtttaa
    16201 ctgattctct taatgcagat aatttccgaa aaatttcacc atttccatgg aaaaagtcta
    16261 atatgtttag ccagatatcg ttaatgaaat ttttaagaaa agctggtgtc aagataggcg
    16321 atgtcataat tattggcatt ttgattatta cctcttttat tcccttattt gtattctcta
    16381 tagaaaagga gaatgaaaca actgaagatg ctatcaaata tgccatcgtt cgaattgatg
    16441 gcaaggaaat ggatcgcttc gatttggata agatcaatca taaactcgtg aattatcatc
    16501 ctgcgaaggg acaatataat attgttgaga ttaaagatgg aagaattcga gttaaagaag
    16561 ataatagtcc tgatcaaatc gcggttagaa ctggatggat ctcaaaaccc ggtcaaacga
    16621 gtatttgttt accccataag ttagttatta gtattgagaa aaaagaaagc acagattact
    16681 acatttacta atctggaaat agatgaggag gtttttaata agaaaaagat attattatta
    16741 ataatagcag gggctttatt aagtgtgtta agcgcttgtg gacaagaaaa agaagcggtg
    16801 acaacaggca caagtatctt taacgggacc tgcaatggtt tagttaacag tcggttagat
    16861 tacttatttt ggctcttcgt caatgagtgt tggtgaagaa attcaataga ataatcgaga
    16921 tactcacacg gctagacagc tatcttgtct agccgtgttg ttgttctggc tttagtcagt
    16981 tgggtagtat tcttagtttt cttaaaatat tttcctcgat ataaaaagat tcgttctcgg
    17041 tagttttgaa agtttctata gccataggca attcgtttta gtactttgat atggttattc
    17101 aaacattcta tagggccgtt ggaataggga acgcgcaacg ctcgtttgat ttctgtttgg
    17161 tatttcttga aggtcgtgaa cactggttgg tagtgctctg gcaactcctt gtagtcttct
    17221 tttaggagcg cgacgaattc aggaacatct tgtcttctga taacggaaag aaaatcctgg
    17281 tagacttcgt agcctcgctt taaggaatca tcataagata atagccgatc gactatctct
    17341 gcttctgtca gatgatctcg aaatgacgga cgccagtagc gatgctgacc tttcaattcc
    17401 cacgcattct tttgaagtag tttccagtat ttctttaagt gcttcgaccg acgatcccct
    17461 tttttaaagg tcttcattac ttgaattcgg tggttggtaa aagcccgacc aatgtgttgg
    17521 ctaatatgaa agcgatcggc gacgattcgg gcattcggaa aacactcttt caccaatgtg
    17581 cgatagggtg tatagaaatc tgaaaccact agacgcactt gttcacggac ttttataggg
    17641 tagcgcaaga aatagtcgcg taaaggattg agttgccgat tttccacaat atcaatcagt
    17701 tgatgggtct tgccgtccat catgacaaag ctcatggctc ccgagacatt tttcactgat
    17761 ttgaattcat cgaaacacac cacttctggt aacagctggc tcaaagaaat ggttttaggc
    17821 cgaaggctgc gaagtattcg aatgacagtg gtcggagaaa tgtgcttcat acgtgcgatc
    17881 aaagacatag aaagaggttc gcttaatagc tcaagaatgg cttgtttgac tctgcgagca
    17941 atggagcagc gacgatcact aactgaatct tctgctaaaa aagtctttcc acagtgttta
    18001 catttgaagc gctgtttgtt aatccgcaga atggtcttgt agttactgac atcattcaat
    18061 aagatggata cttttttcca gccctattta atgatcgttt gttcattttg atagtggcga
    18121 tgaaagcatt cctccggttg ataggtcaat gtgccatcaa aagcaaaaca tgtttcgtca
    18181 ttgatgcgct ctttgcgaaa ggcatcctca tgaaaggtaa gatttaggtc tagaatatct
    18241 agtaactctt tggtaaactc tgaataggac atcttgaaac actactttga atggtttttg
    18301 tcgactttat tctaaaggat tttaaggtgt ctgtttgcgt ttattagaaa aaagaaaaag
    18361 tattggcaac agaaggcgtc accaacacta gatattatag atcccttatt ttcttcgctt
    18421 caaattgtca aggagtcatc ataaattttg tatggattct tttgtttggg tagtagtctt
    18481 ctaaataatc ttggattaac accttcatct ctattttact tttatatttg ttggagtcga
    18541 ttaattctca tttgattgat cgatgaaacg cttcaattac tgcattgtca acaggagtcc
    18601 cagtttggct cactgaatgt gtgatgtggt ggtcgttcaa cagctcttga taaataaaag
    18661 accgatgctg gctacctcta tcggaatgaa ttaccgtagg caattctcct taataaattt
    18721 gtaatggacg gacgacaatc tcttttttta tgtgtgaatc taccatatag taacgtagaa
    18781 tacgagttgc taagtcaata taggtggaca aatagaggcg accgtcatta catggaatgt
    18841 aagtaatatc catcacccaa acagcatcaa tttggttctg ttcaaattat cgattcaaca
    18901 tttttttacc atttctttga ttgttttttc tctaccatga gaagatttgc gtcgatgaaa
    18961 tcctcttgtg cataaattca tttcacgcat aagaacagct acaacacgtt tattgatttc
    19021 gattccttca tcgtaaagtt tttgagtgat ccctggagat ccgtatcgat ctttgtgttc
    19081 gtagaacact cgtcttcctc ttttagtcgt ttattttcct gttcaagaat gtagaaatct
    19141 gctttatcag gtaatctttt tccttgtcca gcaaaagcat ttttcccgta tcgacgatat
    19201 tctgaaaccc atcgggaaat tgttggacga gagatattgt attcttcagc aattttcttg
    19261 gtagttactt ttttctcaag gatatcctgt gaaatcttta gtttaaattt acgatcatat
    19321 gtttttctca atagagttca ttccttccgg taacgtaact gatagtcaag aaaagtgtat
    19381 catagcccaa cttagtatca gggtttgtca actaaactgt ggaagttgat agataagagt
    19441 ttttaacaac caaaattctc ttgactattt ttgaaatcag acaaattttg attggacaca
    19501 tagttgaata aatctaccgt cgttacggaa ggtaaatcgc atgcttttca attctagagg
    19561 agaagtcctc gttgaagtac catagcccag ttctggattc gacctcctgc ccacttcata
    19621 cgtaaataaa gtaattttag gagtgagact gtgacaaaag ttatttttaa gctaaaaacg
    19681 aattaaaaaa cgttgataat taaaatcatt cgttttagat tctatgaatc attatagaac
    19741 gctgtaaaat cgatacttta taattataaa gaagatagtt tcaaatttat tgttcgaaat
    19801 ttgaaactat ctttttactt atgtcacaga ctcccttagt tgagttattc tttaatttcg
    19861 aatttcgaga tagctagata tattgcaagc atacattctt gataacaatc ttgatcatat
    19921 tttccagttc tccaggattg cttatgaatc aaagagctaa atcttttgag aattgcctca
    19981 acagcttctt catttccagc ctttgctaaa atgaaatctt cttttaaaga attcataaat
    20041 ctaccttctt cccatggact tataatctat ttctttggga aggaggagaa cgagcaaaga
    20101 agcatacaaa attagttatt atgcagatat taaaccagca ttttttgaat ttttttgtaa
    20161 agtcgatgtt ttaaatttgt cgcaccctgt cttgtaatcc ccagaacctt tccaatttct
    20221 ttatcagttt tatcaaagat aaatttttcg ataagaaaaa tttgttcagg ttctttcaac
    20281 aaaggaagga tttttgctaa ttttatatct tcaacaagga gagtcacatt taaaacatta
    20341 atcggttgtt taaattttat agtatcttta gtaaacaaat atggtgggag ttgatcaagt
    20401 tgttcatgtt tttgaatttc ttcttgtttt ttataataag tattagcggt gttcaaaatg
    20461 accttttcaa agaatcgaat caactgggca tttacgcgtt cgtcgttatc tgacatattg
    20521 cacacctcct ccattaagat ttaaagggaa aagaggcaaa aaacacgaaa agaagctaat
    20581 gcaattatgc tgcgctgcga ataacttcta taagctcaca tgcattgata ggactgctga
    20641 tgtttatttt caaaaatgtt tttttagttg ataatggata gttatcttta tacttagaaa
    20701 agaaaattca tatatcttct taaatatcaa acatgtcctt atggctaaga aaatgtcttg
    20761 agctttttac gtataataaa tcttttctaa aacctccttt tggataatat tcttattcgt
    20821 gtatagattg tattttttat ttcttccccc ttcataatta acaactagaa aagagttcga
    20881 atagacacgt atttgaacga aaatttttat tggattccca aattggaata tttagaaacg
    20941 aaaaattcct attatctgta tagtgcttag ataataacgg atcataataa ttattgtctt
    21001 tgaactagat atatgtaaat agtattcatc atgatgaaaa atgatgagtt ctcggtgata
    21061 aaatgtactt tttacggtgg aatatacatg aaagcgataa gtggatattt tataatgaag
    21121 taagttagct tattttgaca gaggaatagt attttagata acgtataaaa tactatttaa
    21181 tgatgtagaa aggaaagaga gaaacaaaag tgttttcaga taaagaagtc ttattaaagt
    21241 ctcttcatgt ttgtttaaag ttacctatcg tggtttttaa ttccgaattc agtattataa
    21301 aagagtatcg ctcagataga actacttatt ttttttatga ttataaaaag ttactaactg
    21361 ataatattaa aaaaactaaa gatttccatt tctttactgg tgattttaat gagatgtttc
    21421 ttctatacat gtttaaaacc agatattact tatttggtcc atttagagca aataatattg
    21481 ataaagtttt ttttaaacta aaaatgaaca atttgaatgt tgcaatggct gatagagaac
    21541 gactctataa ttcattgcaa aatttgactt tgtattcttt aggagacatt cgagatattc
    21601 tgattttagt tcattatttt tttactggaa aaatagaaga tctattccat gaaccgttaa
    21661 ttgaatatac agaaaatcta agtaaaacta tcgagcaaat caaaatagat aatctgttat
    21721 cacaaaatta cgatccagaa atatatctgt ttctttatga aaataaaata ctggaatatg
    21781 ttaaaaatgg agatattcga aatttagaaa acatggtttt taatctgagt aatggaataa
    21841 ttccaagtgt tagtggagat acgattcggt ctgaaaaaaa ttattctatc attgtttttg
    21901 aaaagttagc acaaacgtct ataacattag gaatggatat aatagaagcg tatcaaagta
    21961 gagatgcttt gatacaagaa aacgaattag ctgtatcgtt acctgaggtc ttaaaagtga
    22021 gggattctgg gattgtttat tatactaagg aaatcggaaa aactaagata gagcatcttt
    22081 ctcccttaat ttcttctgtc gttcaattca tcggcttaaa tatctataaa agaattactg
    22141 taaaggaaat agctaattat ttttctgtaa gtgaaactaa attaagagaa agctttaaga
    22201 atgaaatgca tattacgatt tataattata tctctaagcg aaaaatttct acggctaaaa
    22261 taatgttgaa atcaaaccat actatcagtg aagtttcttt aggtttagga ttttcagatt
    22321 cttcacactt ttcgagagta ttcaaaaagt acgcaggtgt atctcctaag caatatcaat
    22381 taggtttagt ggacaatttc aacaacatta gctgagaggt tttattttat tatgagacta
    22441 tatcatttac gtagtttgga ggagttgagg aaattggatt ttaacaaaaa atttttttgg
    22501 gaaatttctg atttgagttg tttcgtagaa tctgagcgtc taagtaatgg taataatagt
    22561 gtgttaaatg aatatgacaa agtagtaatt aataaagtaa aaagtttatg tgaagaagct
    22621 agagaatcta tcagtcatat taatggtagc aattttaaac aaacattatt taatctgatt
    22681 aagctagatg ctaaaatatc aagttattta ttctttttga tacatgatga atttatggat
    22741 tatcaaaaat tggatcagtt aattgaaaga gaaagctggg aagattatta cgtgggtcta
    22801 actttttcag aaaagttaaa caactacaag ttaatagatt ataagtattt gagtgaaagc
    22861 aattaattat tcatgtagag tactagacac tttaaatatg gacaattcaa ttataaagaa
    22921 aaagaatcaa taatttaatg aggctgtgac aaatgtcatt ttttgtttcg ctaaaaacga
    22981 gtgaaaaaat gttgataact ccaatcattc gtttaagatt ctaggaatca ttcagaaatg
    23041 ctgtgaaacc gatactttat aattataaag aaggtgattt caaattccga agaaggaatt
    23101 tgaagtcacc tttttactta tgtcacagtc tcattcagta atgcgggata aggctcctta
    23161 ttccgttatt tgaatactct gaagtttaat ctaagaaagg gtaatatttt gaaaacatgc
    23221 ttcatcgcat agaaatgaac ttaatttcag aaagaataca tacatatctt tttatatgtt
    23281 ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag aaatgtactt
    23341 tttttgttgt aatgttcatg aaattctcta agtaaattag ttaacataag gttagtaaga
    23401 ttcctattct gtgaataata cgtatcagat ttatctttta ttcttactag gaaagtaatt
    23461 attaagggag ttgatttgaa taatggttag caagaataat aagagagtat ttctagaaaa
    23521 aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg ttgcatctgt
    23581 tttgattggt gttggtcttg tcttgggaac aactgaacta gttaaagcac aagatgaaat
    23641 ttctccaagc agcccacttg aaacagcgat aagctccgta caagtaggtg acaaagtagc
    23701 ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact tttcggattt
    23761 acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa ttggatttga
    23821 agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt
    23881 agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga aaggaattgg
    23941 atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta caaatatttg
    24001 gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggcagaaa caaccgatac
    24061 gcaaaccgct cctaatgggg taattacgtt tgaaaagagc ctagatgaaa tttttaaaga
    24121 gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact tggtaagtca
    24181 tcaagacgat gataaaattg taccgggtat tgatagtacg ggctactttt taacagattc
    24241 agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt ttaaacatgg
    24301 atcagtaagt gcaaaatatg agaaacctaa tactcaaaca aaagatggtt caggatccac
    24361 aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata atttctcata
    24421 ttcatcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg aacgattagt
    24481 tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa catatgatgt
    24541 ttcgtttgaa tccggaacaa aagtagctga tttagcaatc gagcgacggg aaggtcatga
    24601 aaattatggc ataggttcaa taactaataa tgatttaact aaacttatcg attttgctaa
    24661 tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc cattagatga
    24721 aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat ttggtgaaga
    24781 tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga acacttatgg
    24841 aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg ataaagaaga
    24901 gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat atgatgttga
    24961 cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc acaaagccca
    25021 acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa atgaaaaaga
    25081 tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt
    25141 aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata aaccagaaga
    25201 aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg tggtagttgg
    25261 atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg aagatgcaga
    25321 gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga aaggtattgt
    25381 aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg taaaagcttc
    25441 tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag aaacgacagt
    25501 tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag atgccttttc
    25561 aatttcagat gtctataata aggacttcgg taatgcaagt gttgatgcaa acactggtat
    25621 tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg gaacaattcc
    25681 tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag taactgtaag
    25741 taaagatatt t
//
LOCUS       DACEOE010000022        25501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEOE010000022 DACEOE010000000
VERSION     DACEOE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.023"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1252
                     /locus_tag="IW774_000788"
     CDS             <1..1252
                     /locus_tag="IW774_000788"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="catalyzes the hydrolysis of a monocarboxylic acid
                     amid to form a monocarboxylate and ammonia; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1153155.1"
                     /translation="PLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQAEAIGLVPF
                     GQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVPLAGASDGGG
                     SIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEALFYGMRSIH
                     SGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDFLGKEGHDLT
                     EIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWTLFQYGQKLS
                     AATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAIRAQMEHISD
                     CSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLGIQFMAARGR
                     EDLLFQIGYLFEANHQFHLPNPWK"
     gene            1273..2109
                     /locus_tag="IW774_000789"
     CDS             1273..2109
                     /locus_tag="IW774_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1153156.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            2109..2768
                     /locus_tag="IW774_000790"
     CDS             2109..2768
                     /locus_tag="IW774_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1153157.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            2894..3583
                     /locus_tag="IW774_000791"
     CDS             2894..3583
                     /locus_tag="IW774_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153158.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            3589..4545
                     /locus_tag="IW774_000792"
     CDS             3589..4545
                     /locus_tag="IW774_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1153159.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            4589..5101
                     /locus_tag="IW774_000793"
     CDS             4589..5101
                     /locus_tag="IW774_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1153160.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(5357..5905)
                     /locus_tag="IW774_000794"
     CDS             complement(5357..5905)
                     /locus_tag="IW774_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1153161.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(5994..7175)
                     /locus_tag="IW774_000795"
     CDS             complement(5994..7175)
                     /locus_tag="IW774_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1153162.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(7411..8919)
                     /locus_tag="IW774_000796"
     CDS             complement(7411..8919)
                     /locus_tag="IW774_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1153163.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            9078..9269
                     /locus_tag="IW774_000797"
     CDS             9078..9269
                     /locus_tag="IW774_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153164.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            9472..10389
                     /locus_tag="IW774_000798"
     CDS             9472..10389
                     /locus_tag="IW774_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1153165.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            10382..11455
                     /locus_tag="IW774_000799"
     CDS             10382..11455
                     /locus_tag="IW774_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153166.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            11599..12117
                     /locus_tag="IW774_000800"
     CDS             11599..12117
                     /locus_tag="IW774_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153167.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            12098..12490
                     /locus_tag="IW774_000801"
     CDS             12098..12490
                     /locus_tag="IW774_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1153168.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            12566..13501
                     /locus_tag="IW774_000802"
     CDS             12566..13501
                     /locus_tag="IW774_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1153169.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            13634..14413
                     /locus_tag="IW774_000803"
     CDS             13634..14413
                     /locus_tag="IW774_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1153170.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            14406..14723
                     /locus_tag="IW774_000804"
     CDS             14406..14723
                     /locus_tag="IW774_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1153171.1"
                     /translation="MTKLYLLLLVVCLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            14967..16577
                     /locus_tag="IW774_000805"
     CDS             14967..16577
                     /locus_tag="IW774_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1153172.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            16734..17582
                     /locus_tag="IW774_000806"
     CDS             16734..17582
                     /locus_tag="IW774_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1153173.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            17813..18292
                     /locus_tag="IW774_000807"
     CDS             17813..18292
                     /locus_tag="IW774_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153174.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            18325..20553
                     /locus_tag="IW774_000808"
     CDS             18325..20553
                     /locus_tag="IW774_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1153175.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            20682..22325
                     /locus_tag="IW774_000809"
     CDS             20682..22325
                     /locus_tag="IW774_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1153176.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKINK"
     gene            complement(22398..22943)
                     /locus_tag="IW774_000810"
     CDS             complement(22398..22943)
                     /locus_tag="IW774_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1153177.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(22966..23451)
                     /locus_tag="IW774_000811"
     CDS             complement(22966..23451)
                     /locus_tag="IW774_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1153178.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(23441..24253)
                     /locus_tag="IW774_000812"
     CDS             complement(23441..24253)
                     /locus_tag="IW774_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1153179.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(24392..>25501)
                     /locus_tag="IW774_000813"
     CDS             complement(24392..>25501)
                     /locus_tag="IW774_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1153180.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         8314 a         4665 c         5090 g         7432 t
ORIGIN      
        1 tcctttaaaa atgctcggtc aagaaaaaaa gggatggcta gctacttcag gatctcgttt
       61 atttgaaaca caccgagctt ctcatacatc taattatgtg cggcaagcag aagcgatagg
      121 tctagttcct tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc
      181 agtcatctat ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag
      241 tggaggagct gctgctgcag tttcatcagg aatcgttcct ttagctggtg ctagtgatgg
      301 cggtggttct attagaatac ccgcttcgtt ttgcggattg attgggctga aaccttcgcg
      361 agggacgatg ccagttggtc catatgcttg gagaggatgg caaggagctg ctattgattt
      421 cggcttgaca gtttctatga gagacacgga ggctttattt tatgggatga gaagtatcca
      481 ttccggtgcg ccatatcaag tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg
      541 gttgaagata gctttttgtg ttgattcacc gatccactca cagattagtg aagaggcagt
      601 acttgctgta aaaaaagctg tagatttcct tggaaaagaa gggcatgatt tgacagagat
      661 tccttaccca ctagatggtc gtaaactgat cacgtcttat tatcagatga atggcgcaga
      721 aacagcagca atgatccgct cgattgaaac aggcatccag cgagctgtca gaaaagaaga
      781 acttgaaccc atttcttgga ctttgttcca atatggtcag aagctctctg ccgctactta
      841 catcgattct ctgcatgtat gggatgaagc agcaattgtc atggaaaacc tacatcaaga
      901 atacgatcta ttcctttcac caacgactgc atttaccgcc cctaaaatca gcgaagattt
      961 gcaaagtgat gcgataagag cacagatgga acacatcagt gattgttctg aagtagaagg
     1021 tttagacgtt gtttcttcca tgtttgagga gagcttgaag atcacacctt atacacagtt
     1081 ggctaatctg actggacaac cagcaatcag cttgcctact catttgacaa aagaaaacat
     1141 gcctctagga atccaattta tggctgccag aggaagagaa gatttgctat ttcaaatcgg
     1201 ctatctattt gaagcaaatc atcagttcca tttaccaaat ccatggaaat agcagaaaca
     1261 aggagaagaa gaatgaagaa aaaaagattg tttgctctaa taactagtgc cttttttatt
     1321 ttaagcgctt gcggtacagc tgccgctaat caggaagata acagctggag taatgtagaa
     1381 aaaaagaaag aattagtagt agcgacttct ggcacactgt ttcccgcatc ttattataat
     1441 gatcaaaatg aattagttgg ttatgatgta gatttggcaa aagaggtggc taaacggtta
     1501 aatgtcaaaa tctcctttaa agaatacaac gtggatggac aaatcacttc tgtttcaaaa
     1561 agagaatcag attttgctgc gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc
     1621 tccctttcag agactttcaa atattcattt gacagtatga tcgtacgaaa aaaagatgat
     1681 tcaggaatcc actcacttga agatcttaaa gggaaaaaag cagctggcga acccaatact
     1741 agctatatgc gtttagcgga aaaacacgga gctaaattag tcacatatga caatgccacg
     1801 aatgatcagt atttaagcga tgtagcaaat ggtcgaacag atgttattct aaatgattat
     1861 tatctgcaga aaatggccgt atctgcttta ccagatatcc ctgtaaaaat actggagaac
     1921 gtttatttca atgaaaacga aagtggtttt ttattgaaag aaggaaatga tgctctgaaa
     1981 aagaaaatcg atggcgtatt aaaagagatg aaagaagatg ggacgacaaa gaaaatatct
     2041 gaaaaatatt ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg
     2101 agtgagtgat gtactttcct ctaattgatt tttccctcat gtggacttca cttcctttcg
     2161 tactgcaagg cttggtatac acattaggga ttggcttcgt gtcgtttctt ttaggaaatc
     2221 tggtagggtt gcttttgaca gttttaggtc ttttggattg gctgcctctg aatgtattta
     2281 ttcgttttta tttatcgttc tttagaggca ttcccgcact agtcctgctg tttctgttat
     2341 attttgggtt accttatcaa ttaagtgcct taacggcttc agtcatctgc tttacgatta
     2401 ccagcagtgc atttattggt gaaatctatc gaggatcact agcaggcgtt tcttcaggtc
     2461 agtgggacgc tggtttagct tctggttttt ctttctttgg ggtattgaaa tatatcgttt
     2521 tgccgcaagc ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg gatctagtga
     2581 aaggtacttc gttagcagca acaatcacag tgccagaaat atttcaaaaa gcaaagatca
     2641 taggcggtcg gacattcgat tatttttctc tctatatttt agttgcactg atttattggg
     2701 tcatttgtat cgggatcgaa aaagtgcaac agtatttaga aaaacgttct atgcatcagt
     2761 caatctgaaa aatagaaatg gtgctagcgt ggctggcacc atttctattt tttgtttctt
     2821 gtatattctg cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc
     2881 ggaggtagaa aacatgaaga ccccgctaac catcgaaata gaatcctcgt tataccaata
     2941 ttgcatcgag caaggcggct tggttgtaga agaagtcacg atgcctgatg accaaggaat
     3001 tgtagatacg cttagctgct tgacaaaatt tgatggaagc cgagaatggc gatgctatga
     3061 gctgaaagtg acaaaagcag attttcgctc tacagccaaa ctgtcatttg ttggacatta
     3121 taattatttt gttttaccaa gtaaactata tgaagaagta aaaactgaaa tccctacagg
     3181 aataggtgtt ttagtgtatc gtccgtacac ggttgaagag gagatgcctg cctctggtac
     3241 atttctgatt gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag aagctttgac
     3301 aaaccgcttc atggcttctt tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg
     3361 cgtaaaatat tttccaacag atcaattgta caaagaattg aaaagacgtt cagaaaatca
     3421 agatccattt tctaacgaac gatattatga acggttttta gaggaaatag ctagtcaggc
     3481 aatcaatgag ttgcaagaag aagttatcgc gcttcagcat gattatgaat atttgaggca
     3541 acaacagcag atccgtcggt tgcctacaga gccattggag tgaaaaatat gtactatcct
     3601 tatttaagag gaaaacaatt tgacctatta gcattaaaag aagcactgtc gcgaggattg
     3661 ctctccaata aaatccaacc agtgatcgaa ccagtacgtg attccgctac gcttaaaaat
     3721 gtgatcgaat tatttcaaaa aaagcaccat cgaatcgctg tgatccaaaa tccccaagta
     3781 ggacaattca aattattcga tcagcatgtg cacagttgga cagtcaaaga aaatagctct
     3841 gtcgtccctg cacagatctt aacgccagaa aatctcagtg aagtactgga ttcacctcca
     3901 gcgtttcttg ttttcgatgg acagcactac ccaaaagata cagaggtgtg gaaacagttg
     3961 gcaggagttg attcgaaatt tttgatacca gatactagtc gctttcgcat ctggctgccc
     4021 gaaaacaaaa ttgtgatccg tgactctttt caaacgagaa agcatgttga aagttatgca
     4081 gataaaacag atgacttttt tagtgatgac tatctttttt tccacgcaga cggatatatt
     4141 ggattttctg atttcacgat cgaaggtagc cgttatttcg ataaagggtt ccctagccga
     4201 gcgcttgcta tccatctaac gtatattgat gcttacggca atatacgcgt gaaacatttt
     4261 gtctctgaca gtaacgactc tgctaaagac caagcattga agtttcttga agcaggagat
     4321 aaaatgaaaa aatggatcat gcgtcaccat catcaactct tgattacttc aggaatgatt
     4381 gaattattgg ctctttacca gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg
     4441 tcacttatgc atcatttgga acttattagt cacctgctag atcatccaaa agattggctt
     4501 aaatattatt caaaagttca ggaactatat gaagtgatcc aatgattaga taatgtaaat
     4561 gaaaaaggaa tagaaaagag gaaactggat gtatattcgg caaacaagga aagaagaagt
     4621 agaagaagtg gtagaaatca ttaatgccgc agcggatttc attgcttcgc aaggctctcc
     4681 tcaatggcaa aacgggcaga aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa
     4741 agaaagctat gtattaatag tggaagatca aattgctgga acagctgcat tagtctcaag
     4801 cgtggatccc gtttacacaa aaatagatgg tagttggaaa gaatcaactg gtccttattt
     4861 atcgattcat cgtgtcgctt tagcagaaag tactcgagga aaaaaactag caaaaccgtt
     4921 cttagaacat ttgttgacga ttgcagccag caaaaatatc caagatatcc gtatcgatac
     4981 ttatccagct aatgaaccga tggaaaagac catctattct gctggattca cttatcaagg
     5041 gatgattgaa tttccttttg aacacggcga aagaaaagca tatcaaataa tcatcaattg
     5101 actataaaat catctgagca aagataaaga cattaacact actgaaaaaa acaatcatat
     5161 gatagccaag aggttgtaac agaagtggcc agcttcaaaa aacaagaaag aatttccgaa
     5221 aatgcttctc aaatttttgt ggaattttag cgtattttac gaaatagctg ctttctgaaa
     5281 ctcccttcat cgagaggtcg tgacatagct tttgtcacga cctctcggct tatcctacga
     5341 gtattattcg atacatttat atcaataatc ctttttttat tttatctgca gcttcttgga
     5401 acgctccatc gtttgacacg actaaaagtg aggggatagc tttcaaacgt ttcaactgtg
     5461 ctaaatcttc tacataaacc gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt
     5521 ttttcaggcg cgattgcaat gtttctttag agatcgtaat aaagatagga cggactaccg
     5581 tcccgaaata ctcatataaa tgccaaaaac catttgctgt caaaacggcg taacaatttc
     5641 ctactgtcag tttcttttta atttctgctt tactggaacc atatttatgt tgagcatatt
     5701 ggtcccactc tgcaaactca tttcccttga tcatctgatt gaatgtattg tcagaaacaa
     5761 aataataatc ctcttgatct ttttccccag gtcttggtga tcgattcgtg taagtaatga
     5821 tcttttgttc cggctgaaaa atagcttctg cgagtgaagt ttttccagaa ccagaaggac
     5881 cgataaatac atagcaataa ttcattgtca tttctccttt gatgattgaa caagtgaaaa
     5941 agctttggaa aatatttcca aagcttttaa aagtaacttt gcttttttgt atattatacc
     6001 agcaagccag tcacaatagc acttaaaaag ctgactaaag tcgctgttag caaaattttc
     6061 aatccgtgtt ttgcgacaag atttcctttt tcttcattta atcccttcat cgcaccagaa
     6121 atgatcccga tagaagagaa gttggcaaat gaaacgagga agacagatat aattgctgtt
     6181 gtacgctcag taaaatggaa attaccattt gctaattctg tcatagcaac aaattcattt
     6241 gtaaccagtt tagtcgccat gatactaccg gcatccactg cttctttcca tgggattccg
     6301 ctgatgaagg caagaggggc aaagatgtaa cctaggatct cttggaaagt gatgccgaat
     6361 atcccgtgga agatagcatt gatcatggca atcaaagcaa caaaaccgat aagcatagca
     6421 gcaaccgtga tcgccacatg gaaaccatca agaatgtatt ctcccaacat ttggaaaaat
     6481 gtctgttctt tgttttcttc aatgatcagc tcatcttctt tttcttctaa tacataggga
     6541 tttacaattg aagccagaat ataaccgcca aataagttca aaacaagggc agtgatcaca
     6601 tatttagaat cgatcagtgc catataagat ccgacgatcg acatagaaac tgtagacata
     6661 gccgatacac tcatggtgaa caaacgtttc tcagatagga agggtaattc cttttttatg
     6721 gaaataaaaa cttccgattg tcctaaaata gcagaagcta ctccattata agattccaat
     6781 ttgcccatcc cgttgatttt acttaatcct agaccaacag ctttcataaa taatggaaga
     6841 atcttgatat agcgtagaat accgataata gcagaaataa atacaattgg catcaatacg
     6901 gaaagaaaga aaggtgcact tcctttattt gctacaccgg caaaaacaaa gtcaacacct
     6961 tgcccagcaa aagcaagaag atgatcaaac atttttgcca atacagaaac aaccgctgta
     7021 ccaaaagttg tacgtaataa tacgaaacca agaacaagct gtaaaacgaa catcacaatt
     7081 agtggtttca atttgattgc ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg
     7141 acaaataaac caataatacc taataaataa ctcaaataat cactcctatt ccaaattccg
     7201 ttttatttta cctgttttgc aaataaaata ctaatcttta gtgaaatttt cattactttt
     7261 ttagagtgaa acgcattcat tttccattag aaaataaagc gaacaaaata aaaggtttaa
     7321 aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac tatcgattaa gctaatagtt
     7381 tcgttttcaa catttataag taaacttttt ctattggtaa tatagatttt cggaagggta
     7441 gacaggtgcg caggtaatat cgattcctag ttttttcaat gtttgttcat catttttatt
     7501 gaccatcaca gtagagtggg cttgtgctcc ctcaagttca gctagtttat cataagcaac
     7561 ttgagcgatt ggattcgtta ctgcactgat tgataaagcg atcaagactt cattgatgct
     7621 tagtgcatcc aaagaggaat gaagtgtctt tgatttcaat ccttgtattg tttcaagaac
     7681 taatggtgac aataaaggaa tgtcatctgc gatatgggct aaatgtttta ctgcatttaa
     7741 gataactgcc gctgacgcat ccatcaatga acttgtacgc ccggtaatga tgacttcatc
     7801 tggtaattcg attgcaatca cagcagaagg atcttttgta ggactttgcg cttgaatctt
     7861 atctgcatag tcatgggcag ggactaccac gtttcggtct tcaggacgta aaccgacttc
     7921 ttccataatc agtttgattc gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc
     7981 gcattctgtt tggaaataac gacggatgat ttcttgttta gaggcttctt ggacaacggt
     8041 atcatccacg atcccaaatc ctacacgatt cacacccatg tcagtaggag attgataaac
     8101 agattcttta ccagtaattt tttcaataat acgtttgatt acaggaaatg tctcaacatc
     8161 ccgattatag ttcacagcta ctttattgta ggcatcaaag tggaaggaat cgatcatatt
     8221 gacatctttt agatcgactg tggctgcttc ataagcaatg ttcagcggat gtttcaacgg
     8281 aacattccat actgggaaag tttcgaactt ggagtaacca gctgcatgtc ctttttggct
     8341 ttcatgatac agttggttca aggaagtagc gagctttcca cttcctgggc caggagctgt
     8401 cactacaacg attggtttag ttgtttcaat atactcattt tttccaaaac catcttctga
     8461 gacgattttt tctacgttga ctggatagtc ttcgatttct gagtgtttat agaccttgat
     8521 gtttcgtctt tctagtttat tgataaagac tttcgtggca ggctgtccac tatagcgagt
     8581 gataacgaca ctgttgatta ataagccata accacgtaat tcatcgatta accgcaagat
     8641 atccatatca taagtaatcc cataatcacc acggatctta ttacgttcaa tatctccagc
     8701 atagacgcaa atcagtattt cagcttgatc tttcaatttt tgcagcaatt tgattttcgc
     8761 atcttcttca aatcctggaa gtacacgttt cgcatgcttg tcgtcaacta gcttcccacc
     8821 aaattctaaa tataatttgt catagtggtt cactcgttcc aaaatatacg ctgactgctc
     8881 ttcaatatat ttttctgaat caaaaccaat ttttttcact aggttccctc cttaagtact
     8941 aaagcatttt ttcatttttt tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa
     9001 acaaagaaaa tggtttaaac caacttgtct agatagttat tcattggtat gatagttttt
     9061 gataaggaag gtgctacatg aaaaaaagac agaaaaagaa aaacgcatat aaacactata
     9121 tccgttcgat ttttaccggt tatgaaagaa tgcttgaaga tcccgaattg gaacaattga
     9181 cgtttactta tctaaacgaa gaaacgcagc taactcgtga tgagcatcag cgcattcatt
     9241 tcacaacaag agatctgcct agtaaataaa aaataagata aaggtcatcg gattcgattc
     9301 tgtcctatca gatggaaaga ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca
     9361 gcctgtttta tggagcattg cccgctcttt tttataacaa ttttttacag aaaatggtac
     9421 tattgaagta ttaataagtg aaaaacagat gagtaagagg aggcaagtag tatgaaagag
     9481 ccagatacaa atgcaaatta tgagccagac gaactagagg agacgaatgc ggatcaggat
     9541 acaggacaag aatcgtcttc tttttacgat atcaaagaaa aagcaagcat gaatctttcc
     9601 caattattag aagacttaca ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag
     9661 atctaccgtt tatacaatgg ccgattgaat gaagaattaa aaagaagctc aaatgaaaat
     9721 cacgagatcg atcaattgct gatgcgtaag atccatgatc gttttttaca ggaatttcct
     9781 tttatggaac aagtagaaaa catctcgcca acgatgagct attacaaaat cggcacttat
     9841 taccatgatc ggcccacggt cggtattgat gcaagtctgc cagaaatttt tgttttgcct
     9901 aagatcgatg aagagtggga gaaatattcc caaccggagt caacggacta tgacaaaaaa
     9961 atcaatgagc tagacgcaaa ggtgatcact gctcagacag aaattgaacg tttagatgaa
    10021 caaataaaag aaatcaatcg acaaatgact gatttgaacg acaataaagg attcttgaat
    10081 cgtaaaaaaa tcgaggaaga aattcaagaa ttagagaaga agaagcaagt actatctaat
    10141 gaaaaattag gctggcttcc ttatattgag acgcctgaaa caatccagca gcaaaaagaa
    10201 gcattgaagc aagaagcaag agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa
    10261 aggcaaatca gacgctattt cggtagcaaa gagggattcg gtcaagcaat ccatgaattc
    10321 ttgatgaatt atcttggcaa tgagaatcct aataatcaat ctaatgaagg aggttccgag
    10381 tatgaatgaa cacgatgaag cagaatggac aaaaaatgaa gaacaaacag aagatcatga
    10441 agaaacgaac acttcattgg aagaacaaac aagtaatcct ctggatgaca cagaagaaat
    10501 ccctatgcta gaggaaacaa aaatacacga aaaagaaaaa gtaactccga cgaatacgaa
    10561 tactgcagaa aacacccatg cttacgaaat aactgaaaat cccgggtatt tagaaggaaa
    10621 agagcaagat catcaagata tcgtcagttt gattgaaaca gcggaagcag aattagcgga
    10681 tttacgtatg agaaaagatt tgatcgaatc agattttcca caattattag aatcatatga
    10741 tcgttttata ttgaatgaag aaccaatctc cacgatcgga aagacaagcc tgatcgaata
    10801 cttcacgtat gaattactga aaccgttgaa tgatattggt ctggaacggt caaattcagc
    10861 ttatgacaca tggaaaacaa gacatttttc gatcaattat acgttgaatg gacaaaacga
    10921 attagtcttt tcctttgtat taccgaccat cgatcaacga taccaagtgg aatcgatgag
    10981 tctcctaaac gtatcaccag agacaatgga aatcaatgtc caagatgacc gggtgctttc
    11041 tttgatccga cactggtcag tggatcgagt attctctgca gggcagatca cgatattcaa
    11101 tcacaaattg aatcaattat tagcccatgc aagaacactt ggttttaccg ttaatcagac
    11161 attactagac aatacgaagc cacttcgttt gaatcttcag agcgaatttg aattgacgaa
    11221 tgaagtattg gatgatattt ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa
    11281 gatcaacgaa actacttatc aagtacgatt agataaagga cagtctttaa cgataacaac
    11341 aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga gactacccac gatctgtcat
    11401 tgatttcttt atcaattacg aattcttggt cccattaatg gtgagaaagt cctgattgga
    11461 taagttggat taccagatgt cagataattc tgttccaaag gacttgcggc agttatccag
    11521 aataattgtc acaagtcttt tctgtatgta ctaaatggac gtgaataact tgctgatgaa
    11581 ggagggattt cattaggaat gtccacaaat aaaatagcac agcttagctt attgtctgct
    11641 gcttgtgtag caggtaggat cgcttttcaa tttattccga atttccagcc aatgactgct
    11701 atttttttat ttatagtact ttacttaagc ttaaaagatg cgttaattgt catgagtctt
    11761 tctattgcga tcagcagttt ttattttggt gttggaccat gggtcatcgg ccaatggatc
    11821 agctatactg ttgttctgag tttgttttca atcgtctgct tgagaaaaac agtgcaaagg
    11881 aatctatggt taaagggagt ctgttttttt ctggcgggca tggtttacgg aataggtatg
    11941 accgtttttg atacgctgtt atatcgtttg cctcaacctt ggatctatta ccttcaaggg
    12001 gtgtcgtttg atttgatgca cagtataggc aatgttgtgt tctttcttgt ttttctgcca
    12061 gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg aaaaaaataa tttatagttt
    12121 gcttgcaatg agtgtcagta tactcatttt gacaagttgc agtacaacct atacacaatc
    12181 ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa gcaacaatca cgttacaaga
    12241 aaatggtcaa aacttttcgg aaaaagaagt gttcttcaaa aaaggcgatg atttactgag
    12301 tattttgaaa cgaaattttg aagtcaaaga agataacggc ttcatcactt cgcttgaagg
    12361 gcactcacag gatgagaaaa atcaactcta ttggatgttt actgtggacg ggaaaagtgc
    12421 tactactggt gccaaagaga tcaaattgaa tgatggacaa tcggtcattt tcaatttgtc
    12481 taaactataa gtttcataaa aaactgttaa agggcgaata ttgccataat tcagtgagtt
    12541 cagttgatga aagaaggaaa ataacatgga aaattttaaa aaaagcggaa tgttttccgt
    12601 aattgctgct ctaggggcta atattctagt tgctatcagc aaatttgtcg gctatgcaat
    12661 cagtggcagt gccgccatgc taaacgaaag catccacagt atcgttgatt gcagcaatca
    12721 gattttgctg ttattcggtg ataaacaagc caataaagga cagagcgagt tgcatcaatt
    12781 tggtgaagga cgagcaaaat actttttcag tacaatcgtc gcaatgatgc ttttcttcgg
    12841 cggtggggca cttggggtga tggaagcttt tgaaaagttg acgcatccat cccatgaagt
    12901 gggtaatccg atcattgtta tagcaatact gttatttggt ttggtaatcg aaggaagttc
    12961 gttacgcgtg gcaatgaaag aaatcaaaga attgaatact gaaaaacttt ccacgatgcg
    13021 ctttttgcgc gaaagtcgac acagtgaaat actgattatt tttacggaag acttgtgtgc
    13081 tgtaatcggt ttggtcttgg ctttagcagg aacaatattg acttatgcaa ccgggattgc
    13141 tgcatttgat gcgatcagcg gattgctgat cggtttcttg ctgatgggag ccgccatttt
    13201 cctagcaaaa gaattttata gtctgatcat cggtgaaagc gtgacagcaa gcgatttatc
    13261 aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg ctgatcgata taaaaacagt
    13321 tcatttaagt ccaacggaga ttttagttgc agcaaaagca gatatcgttg gtaacaaaga
    13381 gaatgaagct tactcggtca tcaatgatat tgaaaaaaat atgcgtcagt ctcttcctga
    13441 caagaagatg tatatataca ttgaaacaga taaatatgac ccaaattatt ctcgtaaata
    13501 aaacgtttat tcatgaattg aagcaaaaat gagaaaaata taatcttgtt tttaaataaa
    13561 acggctacaa tttagcgaat ttatgttaca atgagtccgc tgtttatttg ttgtaattaa
    13621 aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg cttcaagctt tgcatgcagt
    13681 gatgccactt tgtataagct atataccagt aggattagct tgcggagtat tgttgcaaaa
    13741 agttggattt gatcctctct tatcaggact gttatcgatt ctggtctttt ccggtggcgc
    13801 gcagttttta gttgcatcta tgcttacgac gcaagcatca tttgcaacga cattattgat
    13861 ggttttcttt ttagaattac gctatacatt attaggagca agtcttgccg gttttatgaa
    13921 aaaagaaaag cggacgtttt tggctgtctt ttctcaatca ctgaatgatg aaaactatgc
    13981 ggtgaactac ttaaagtttt caactgaccc ttcctggaat aaaagaaagg ctttatatgt
    14041 caattggttc tcaatgactg catgggccgg tagcaatatg atcggtaatt ttttcggctc
    14101 ggtgattcga gtagatgctg accttgtcca ttttgctttg acggccatgt tcattttcat
    14161 gtttatcatg cagatgaaaa gcgcactact gatctttaca ggtttattct caggcgtatt
    14221 aggggtcgta ttcatggtat tattccaaaa tactttcgga ttgattgcag caacagtcat
    14281 tgcttcattt gccggattta tgctggagaa ggcattcaaa aaagaaaaag ctaagaaact
    14341 aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac ttcccggaac aggaggccaa
    14401 tcaccatgac taaactatat ttgctacttt tagttgtttg tctgttcatc gtggcatata
    14461 tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa agtgccatcc tggttcagtg
    14521 actggatgaa atatgtaccg gtggcattat ttgcggcgtt agcttttaaa gatgttttca
    14581 ttactcatga acatctggat attgcttgga atatcaaaat agctgcaatg attctagtag
    14641 caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt tttgacagga cttgcgtctg
    14701 tctttttgct gtctatgctt taaactgttt tattttatta aaaacagaga ttgcgacata
    14761 agttgttcaa actgatctat ccgaactgca tttgcaacag tcaattctgg atgcaagttt
    14821 attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc aagctctttt ttattttagg
    14881 aattagagag ggatttgtcc ttctatgatt ttttgctata atagggaaga tgtttatttt
    14941 atgaaagaat agttgaggga atttaaatga ataaaaaatt gatgcaagga acattttggc
    15001 taacctttgc taatcttcta tgtaaagttt taggggtagt ctatttgatt ccctggctaa
    15061 gtatgatggg gaacaatcaa gatggtatgc ttgctacgac attgtataat gttggctatc
    15121 ttccttatgg attgttcctg atgcttggta cagtcggatt tccaaatgcg attgcaaaaa
    15181 aagtagctgt agccacaaaa gaaggagata acgctgcttg tcggctgatt tttagaagta
    15241 cgatcaatat catgtttgtc atcggtatcg cctctgctgc actgatgtat ttatttgctc
    15301 ctttactagc cggaatcagt ccgattgcta acgttaataa tgggatacta gctatcagaa
    15361 gtttatgccc atctttgatt gctatcccaa ttcttagtgc aatgagagga tatttccaag
    15421 gaaaaaatga tctgcggcct tatggaactt ctttgattat cgaacaggct gtgcgagtga
    15481 tcgttatctt ggctggtact tattacttgc gcgtattgac tgacggaacc atattagaag
    15541 ctgtattgat cagtacagtc gcttctttct tcggtgggtt agctgcgatt atccatatgt
    15601 atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga ttttttgatt tccagccgtt
    15661 atttcgaaag agaaaaccga tctgcctctg tctctatcat tcgggaaaca ctaccgttta
    15721 tttttgtcgg ctcagtcatt actatcgttc aaatgatcga ccaagtaacg atgaaaccac
    15781 tgcttcactt tttccgtcca gagatcgcag atcagcaact ggaattctta ttcagccgtg
    15841 cttctgtcaa tccaaataaa ttgacattga tcctgatctc catggtagga acagtagcta
    15901 taagcagctt gccaatatta agcacgatga agaaaaaaga tcggatacaa atcgaaaaaa
    15961 cagtaggaga tagtttttcg attgcgttat tgattcttct tccttcattg actggaatgt
    16021 ctttattagc tggaccacta tacacattgt tttttggcta tgatcctgaa agtgtcggat
    16081 atttccaaat ggctttgcta gcttctttgt ttttctcttt attcacgatc ctatctacga
    16141 tgcttcaatc tttgaatcac catcgttttg cgatccgatt aactgtagaa gcaattatcc
    16201 tgaaagtcat cttccaagta atcggattag gattagttgg cggatacggg atgagcttat
    16261 caaatggctt agcattcggt attgtctttg ttcgcggcta tcactttatg tgcaaagaat
    16321 accgaatagc acctttagca aagatcagca attttttctt aaaaactttc cgctccaccc
    16381 tgattatgct tgcagtatgt tttgccgttt tctttttgct gaatcagcga ttgtcaatgg
    16441 aatcaaaaag ctatgcaatg atttattgtg tggctgtagg aagtattggc ggcttagtat
    16501 ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt gaaaaatgtt aaacatcata
    16561 gcagaagcaa agaataggtt aactaatcgt tttttgctgt cacttgaaaa acggcaactc
    16621 gtatgattac gcttgtcgtt tttctatttt ccaattttag tttcatatct aatcttactt
    16681 atgatatagt tataaggtct ggaaaaattc tttgaaacga ggaaattaaa cacatgaaac
    16741 ttgcagtagt tacagatagt tcggcatatc tgccggaacg tatcaagaac catcccgact
    16801 tattcgttat tccaatccct ttgattatgg atgggaaaat ttataacgaa ggaatcgata
    16861 tcgaagcgga tgaatattat ggacttttaa aaaacagtaa agattttcct actacttcac
    16921 aacctgtcgt aggggaagtt ttgggtttat atgaggaatt gaaagagaaa gggtatgaca
    16981 cgattttaag tatccatctt tcttcaggga tttctggctt tgtcaacaca ttatttgcga
    17041 tgaaagatga catcagcgga gtaacggtca ttccttatga ctctaagatc actagtatgc
    17101 cgatgggaca catggtagaa gctgccttag acttaaatga tgaaggtcag tcccttgatg
    17161 aaatacttgc acatatcgat cgaatccgag ataataccta cgcttatctg attgtagatg
    17221 atttgaacaa tttagtacga ggaggacgtt tgacaaatgg tgcagcgctg attggcagct
    17281 tattaaaaat caaaccgatc ctgacatttt ctgaagggaa aattgtttta ttcgaaaaaa
    17341 tccgttcaag taaaaaagca tttgctcgag ctgaagaaat tattggtaga agaaataacg
    17401 aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa taatttagaa gtagcgattg
    17461 cggagaaaga gaaattgcag aaaaaatatc caaatgcaga aatcgaaatt ggtcatttcg
    17521 gaccagtcat tggtacacat ttaggtgaaa aagccatcgg cattgccatt tcagcgcaat
    17581 aattcctttt attcaaaaaa cataccaaaa atattccaac atcatcttgg actttctaca
    17641 tgttactaat gagcgtgtaa aagaacgaga tggtgttttt atttctatca atgaagctct
    17701 ttttctttgc aatcctcact tcaatcctct ataatgtcgc atatagttta gtaaatgagt
    17761 aaactactaa tttacttatt tactaaaaat aaaaggatga ggagttaaaa taatggaaat
    17821 accaacaaat ttaaaacata aaccagtaat cgttgtagaa gactatgata aagttgatgg
    17881 aagaaatgca ttaaatacgg atgcaaaagg gctttcactg ggccttgctc agtggaatga
    17941 tcgtggaaaa gtcgatatct caggcaaggt atggcgacat actggagaaa aatggtcacg
    18001 acagtcggaa gaattaccaa tcacacgtat tttagattta gcgatcttag ctgcacaagg
    18061 aagtttatac ttccaagatg cttatcgtta tgaaaaattt tatgatccag aaaatccagt
    18121 cgtagacatc attggattac aagggaaccg aatgacggta gaagtagata ccaaaaatcc
    18181 aaagatcgat gaagacatca ttttgtatta cgatacacta caaaaagacg gagaactgat
    18241 tggggaacga ttccgtattt taaaacgtct tttagatgag ctaggatatt aatagacaga
    18301 gatgatcaaa aggggaagag aagcatggag aatttctcac taacagaaaa ccgaaccttt
    18361 tcacggctag aaaagcaaat gatctggaag aaaccgaaaa gccatcggac aagtgcagaa
    18421 gaatggcgga tcgcctccga aatcaaaggg aattggcaag atcctgaaat gaaaatcagt
    18481 aacgtacttt tggaaggaga tgccgggtcg gggaagacac aattagccaa agcactatct
    18541 gctgatctgc aacttcccta tacaaaagtg acctgttttg cagatatgga caaatcagat
    18601 gttttcggag ccttattacc tgtagtagat tctaataagg aacaagacca agaattacta
    18661 gaagctattt accaaacaga tacacttgaa gctgtactga cactgatcca gaaccattac
    18721 ggcattgatc ctttttctgc taaagaaaaa ttagcagagc tagttcaaag gatcgaagct
    18781 gattatccag atgcaatcca ttacagctat tacccttctg aaatcgttcg agcgattgaa
    18841 aaaggatatt tattagagat tcaagagccg actgtcatcc gagatgcctc tgttcttgtg
    18901 gcacttaact cagcgctcga accaaatggg atgttgaatt taccgacagg aatcattcgt
    18961 cgccatccag attgcgtcat tgtgatcact acaaatcgaa attaccaagg aaatcgccca
    19021 ttaaatgaat ctttaagaga tcggatgcaa catgcggaaa aaatggatct tcctgcctta
    19081 gaagtcatgg cagaacgtgc gatggcgaaa actatgatcc aaaaacaaga acttctctta
    19141 aagatggcag agattatccg tttgttggat gaaactgcaa aagctaacgc catcaaaggt
    19201 gtagcaggaa tgcgttctta tttttattgg gcaaatacct ataagcaagg acaagattta
    19261 ctccagtcga tttatccaaa agttttgtat aaattatcca ctgattcaga tgaattggca
    19321 attcttgaac aagctttgtc tgacagtgga ttgctactgg aacttcagga catattgaga
    19381 aaagaaaaat ggggtcatcc tcaatctgag catacaaaag gaagaaccat tagcgccgaa
    19441 gaagcaaatg aacgaaacat cactgaaaat gctgaagaaa ttctgcgctt agatgaaaag
    19501 aaatcgttgt ctgctgaaga accaaatgag ggagaagaga aacaagaaga aacaatagaa
    19561 aaaaacgaga ttcctcctca aatgcaaaaa gaacgcagta gcgatatcga aggaaaaaat
    19621 caaaaagaac aacaaacagg agatgattct caagaaatgt ctgcttctga catggaagca
    19681 catgataagg aaatcaagaa gcaactgaat aaagaagcgc gacaaatcat gaaggaaacg
    19741 attcatgaaa aagaaggtct catcgttcac cgaccagctt tccaaggaaa aacgccggaa
    19801 gcacgtgagc tacacatgca agtaatgcca atagtagaat cacttagccg gcaaatttta
    19861 gagttactgg ataatgaaca aacagagacg taccaaaaag gaaaatacga aggtcagcgg
    19921 ttcaatgcaa gcaaagtagc atatggtgat ttgagaaatt ttgacaaaaa gaacccgcca
    19981 catgagcaac cttctttagc ggtagctgta cggatcgatg aatctgggtc aatgatacgg
    20041 gatgatcgga tcgaagcggc aaaaaaagca gcgattgcca ttgccgaatt ttctaagaaa
    20101 gtcgatatcc cattgttgat ttacggagat acagcagacc gttcgtcaag agaaaagact
    20161 tctttgtttt cttataaaga atttgaagac ggatttcaat ggttaaatga aaaattagtc
    20221 actatgaaac caaggcaaaa caatcgtgat ggtgctgcac ttcgtttagc agcagaaaaa
    20281 ttaaacagac aatccgcaac aaccaaactg ttgcttaata ttagtgacgg tcagccaaaa
    20341 gcgttgccgg attatacagg caaaaaagca aaagaagata tccagtctgt tttgaaagaa
    20401 tatgaacgtc aaggaatcct atttgtctcc gcagctattg gccaagacaa agaagaaatc
    20461 aagtcgatct acggtgaaag tcgttttgta gatattactg acttgaatga atttcccaag
    20521 caaatgattc aactgattgc ccgctatcta tgataaaata aagctcaacg aacgcactta
    20581 gcagattcgt tgagcttttc gtttctagtt accggtaaca aggatacttc taaaatagaa
    20641 tcggttataa tcaagaggga ccgttaaagg agtgaaacga tatgatacaa gaacaaaaat
    20701 ggtggaaaga agcagtaggc tatcaaatct atcctgctag tttcaaagat agcaatggcg
    20761 atggtcaagg agacatcaat gggatccgtg aaaaacttaa ttatttgaaa gatcttggta
    20821 tcggctttat ctggatcaat ccgatctatc aatctccatt tgtcgataat ggttatgacg
    20881 taagtgacta tcaagatata gaagagagat tcggaacgat ggaagatttt gatttattgc
    20941 taaaagaagc acatgaatta gggatcaaag tgattatgga tcttgtcatc aaccatagtt
    21001 cggatcagca tcaatggttt gaagaatcga aaaaatctaa agaaagcccg tatcgcgatt
    21061 attacatttg ggtcgacggg gtagatggca aagaaccgaa taattggaca tcgattttcg
    21121 gcggttctgc ttgggagtat tctcatgaaa ctggacagta ttatctgcat gtttttgcaa
    21181 aagaacaacc agacttaaat tgggaaagcg agaaattgaa agaagaacta ttcaatatga
    21241 tccgatggtg gttggataaa gggatagatg gatttcgttt agatgcgatt tctcatgtaa
    21301 aaaaagatga atattcagta aaagccacag agaacccttt ctcacccttt caaaatgtct
    21361 caggaatcga agaacatttg accgatttga aacatgtttt tgaagagtac gatatcatga
    21421 ctgtcggaga agcaagtggt gtcactgcag aagaaggacc acaatgggta gggaaagacg
    21481 gttatttcga tatgatattc gaatttgatc atatccatat ctggcagcaa gaaaaagaag
    21541 ggcaattaga tgtccttaaa ttgaaacttg cattaagcgc ttggcaaact tcattggatg
    21601 gtatcggatg gaacgcttta tatatggaaa accatgatgt tcctcgtgcc gtttcagtgt
    21661 ttggagatac tcgtcccgat ttttgggcta tgtctgcaaa agcaatcgct atgatgtatt
    21721 tctttttgca aggtacgcca tttatttatc aaggacagga aatcggaatg acgaatatgc
    21781 cctttgaatc gatcgatcaa gttgatgcag tagattcgaa aagactttat aaaaggctgc
    21841 ttgcggaagg taagacaaga gaagaagcat tggatatcat tcgtgaaaca acgagagaca
    21901 acagcaggac acccatgcaa tggacttcgg aacaatatgc tggtttttct acacatgaac
    21961 cttggctgat cactaatcca aataccaaaa cgatcaatgt agaacaacaa gaatatgaac
    22021 cagagtcggt cctgcaattc tataagaaca tgatccgcat tagacaaacc aataaaggtc
    22081 tgatatacgg ttcttataaa gaatatcttc atgaacatcc acaactttat gtctatgaaa
    22141 gatatttaga agatgaacaa tatcttatta tggtaaactt aacagaatca cttgctgact
    22201 atgaacttcc aaaagaagca gatcagtcct ggacattact cttgtcaaat tcctctagcg
    22261 gagaatttga agctaaaggc atactagcac cttatgaagc tcggttatat aaaataaaca
    22321 agtaaaactt cctcgttaaa gttcattagt tggactttgc gaggaagttt ttcagattgc
    22381 tctgtcaatt actcatttca ataattcgct ctaggaccac cgatatattt aggacgagag
    22441 cgaagggctg tcacgtgagc ttgtccaagg gttttcaaac gaggtctgac aggtacttgg
    22501 acatgtttga tatgcatacc gattgatgtg tcaccgatat cgataccggc ttgtccaact
    22561 acgtgttcca cttcgacagg atcattgaac tggtcaaaag cagcaacaga acaagcacct
    22621 ccagcatgta gtgcaggaac aacagagaca atttcgaaat tttttgcttc tgccactgtt
    22681 cgttctacta ctaatgctcg gttcaaatgt tcacaacctt ggacagctag cgcaatttct
    22741 ttaggatcca acaactcttt taacgtacgg atgatccatt gaccaacttc cgcactggaa
    22801 ttttttccaa tatgtcctcc aacgacttcg cttgtgctgc atcctaaaac aaaaagatca
    22861 ccttttttca agtgtgcctc agccaatatg tcattaacta tttcagtcaa ttggtctttt
    22921 aattcttttt ctgacaactt cattttctaa aaccttcttt ttcaattaat attgcttccg
    22981 tgcttgaata cgttgaagac ttgcagttat aggaaaggca agtcccattc caatcagatt
    23041 ttggaagatg tttcctggta tagaagccac tccagccggc caatcatata aaatccaacc
    23101 ggcgatacca tagccgatga tcataactaa gcttcctaat aaaagcccta atccccacat
    23161 acgagtagaa gatttgctga aagaaccagc aacatatcct tgtaacccgt ggatcactaa
    23221 tgaaaaaact gcccactgcg gatatcctga aatcaaatcg atcaatagac cactggaagc
    23281 accaacaact aatccacccg ttggaccata aagaaaagca gcggtataga tcccggcctc
    23341 gcataatgtc acaaatccat tcgtagccgg tacagggatc aaaattgtta atgaaagggc
    23401 aactgtcaac gcaatcagca tagcatagat cgttaattgc ttagttttca tttctcattc
    23461 ctcctttttg aataccgcca gaccagacac ttccggtatg atcgttcatc agcacgccat
    23521 tagaaataca atgggcaaca aattttttac tttctgctac tgctttggta aaatctgccc
    23581 ctaacacaag attcgctgtt attgcagaag aaaaggcaca accggccccg tttactgttg
    23641 tagtgtcgat ttttttatga gaaaatacct cataatgatc aattgaatag agaagatcca
    23701 cagcttcatc tccagccatc cttgtgccgc ctttgatcac aatgtttttt gttcctgaag
    23761 cagtgagttc tttagcgaga tggatcattt ctgttaacga agagggaaga gcttgaccag
    23821 aaagtaaagc agcttctgcc aaattaggcg taatcatcgt aacaagtgga aaaagttctt
    23881 cgatgagctg cgaaatatac aattgattga tcacagcttc agtttctttg aatgccaaaa
    23941 cagggtctaa aatgattggg atattttgtt ttgtttttat gaagtctcga acaaccatga
    24001 ttgcttcact tgaattcaat aacccaattt tgataccatc taatgaagtc attttatcaa
    24061 tcgaaactaa ctgtcgttta agcaattcca aagatataga ttccagcata aatcgctcat
    24121 taatatctag tgcccctata gaagtcaagg cgcttagtcc gaatagacca aaattctcaa
    24181 aagtttttaa atctgtttgg attcctccac cagcaaatgg atcagaaccg ccaattgtca
    24241 agattctttt cataacattt tcctccatga ataagagtat aacgaaattt tcaaaaaaga
    24301 aaaacagcca attggcatat ttttatctca tccaatttaa aaaaacaaaa agaaaagaaa
    24361 gaccaaaatg gatctttctt tccttaaata gttaggactt ttgtgatttt aattctttct
    24421 cgatttgaag ccgttgcact ttcattgtag ctgttcgcgg gattgcatca aaatcacgga
    24481 tgatagggac gttcaagcga ggaagttcat ggacttgctc ccaccatcga ttccaatcca
    24541 tttctttatc aggagctaaa gcaataatcg gttgtggaga atgattttta tcccttacaa
    24601 tcactacttc tgccaaaaaa tctaacgaat ctaataaaaa gtcttcaata gctaatgtac
    24661 tattgatcgt ttcgatcaaa tccacttgcc gatctttcaa aaataggtgg ccatgcttat
    24721 ccataaaacc gtaatctccg ctatcccacc aatcgccata aacattttcc tggaaccgtg
    24781 catcttcctt gtaataagtc aaagcccgtc ctttagacag taattgtata tgaccatctg
    24841 tgttttcagg taggacattc ccaactgaat ctgtaatccg agcgcttgtc atgtcttcca
    24901 gtccaacgcc catatctctt gcatcacttg ttttcaaact ctctagtgta tgagccttaa
    24961 gaatcattgg tccgcattca ctttggccat atacttgaag aaataccgca tcctgtgcca
    25021 tcgacgttcg caaaaatgtc ttcattgtct ggttgtttat agcatcaaaa gtggagtgat
    25081 agtatcggat acccgaaaaa gcttctggat gctccttagc agtaaaacgc cactgaacaa
    25141 agttattcgg atgggtctct agtgcaattg gccggtgagc ttccaacata tgaacaactt
    25201 ttgaactttg tgcgtttgct aaaggcatca taggaaatcc catggacatc aaagaagaga
    25261 taccaatatt gaatctggaa tgcactggag agatgtgaaa agcgattaat ttcttttccg
    25321 aaatttttgt gaaaactgtt ttttgccatt tcgtccgcca tcccatagaa tgagcagaat
    25381 gacaaataag tttagggata ccagttgtac cagaagtatg ggtcatataa gaaatttcat
    25441 ttttgtctaa aaatgattga gaaacagaca aagcaggttg ttccattaaa taaagaatag
    25501 g
//
LOCUS       DACEOE010000023        24892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEOE010000023 DACEOE010000000
VERSION     DACEOE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.024"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..742)
                     /locus_tag="IW774_000814"
     CDS             complement(<1..742)
                     /locus_tag="IW774_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1153181.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSN"
     gene            963..2177
                     /locus_tag="IW774_000815"
     CDS             963..2177
                     /locus_tag="IW774_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1153182.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2199..4214
                     /locus_tag="IW774_000816"
     CDS             2199..4214
                     /locus_tag="IW774_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1153183.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4297..5670)
                     /locus_tag="IW774_000817"
     CDS             complement(4297..5670)
                     /locus_tag="IW774_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1153184.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(5696..5980)
                     /locus_tag="IW774_000818"
     CDS             complement(5696..5980)
                     /locus_tag="IW774_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1153185.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(5982..7997)
                     /locus_tag="IW774_000819"
     CDS             complement(5982..7997)
                     /locus_tag="IW774_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1153186.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8176..8481)
                     /locus_tag="IW774_000820"
     CDS             complement(8176..8481)
                     /locus_tag="IW774_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1153187.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(8570..9601)
                     /locus_tag="IW774_000821"
     CDS             complement(8570..9601)
                     /locus_tag="IW774_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1153188.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            9808..10683
                     /locus_tag="IW774_000822"
     CDS             9808..10683
                     /locus_tag="IW774_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1153189.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(10835..11677)
                     /locus_tag="IW774_000823"
     CDS             complement(10835..11677)
                     /locus_tag="IW774_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1153190.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(11769..13646)
                     /locus_tag="IW774_000824"
     CDS             complement(11769..13646)
                     /locus_tag="IW774_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1153191.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(13643..15058)
                     /locus_tag="IW774_000825"
     CDS             complement(13643..15058)
                     /locus_tag="IW774_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1153192.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETEMVQITLHKLLFPNGQL
                     PKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGASS
                     GMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVVLP
                     VHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVNIL
                     DYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKYAG
                     KKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPDKR
                     LAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLKEI
                     EAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLGVV
                     GMVFASFAIWLFIKKRTGVKK"
     gene            complement(15061..18450)
                     /locus_tag="IW774_000826"
     CDS             complement(15061..18450)
                     /locus_tag="IW774_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1153193.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            18707..20099
                     /locus_tag="IW774_000827"
                     /pseudo
     CDS             18707..20099
                     /locus_tag="IW774_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(20172..20819)
                     /locus_tag="IW774_000828"
     CDS             complement(20172..20819)
                     /locus_tag="IW774_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1153194.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(20827..21513)
                     /locus_tag="IW774_000829"
     CDS             complement(20827..21513)
                     /locus_tag="IW774_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153195.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(21519..22592)
                     /locus_tag="IW774_000830"
     CDS             complement(21519..22592)
                     /locus_tag="IW774_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1153196.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            22814..23488
                     /locus_tag="IW774_000831"
     CDS             22814..23488
                     /locus_tag="IW774_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1153197.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            23493..24833
                     /locus_tag="IW774_000832"
     CDS             23493..24833
                     /locus_tag="IW774_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1153198.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
BASE COUNT         7296 a         4659 c         4603 g         8334 t
ORIGIN      
        1 tattactaag atagaccgga ttgtagccaa aatgagctgc tgtttctttt aacgtacaag
       61 aagtaaaatg cttatcgata taatctaata ctctaagcat ctcattttct gcgcatccat
      121 ctgctgatga aaagctttgt tgttctaata aatgagtcaa ttcgatcagt aatagtgatg
      181 tatagagatt taacgaacgg atcttatgac tgtatgtatc aaaataaatc aacagcatat
      241 tttcaatcaa atgcgtcaaa cgacttgttt ttccgccgcg aaaatgaatg aattgtgtat
      301 gttccccttt ttcttttgat gcatttatca aaaaagaaga aagtagatta tttgtgcttg
      361 ccaaatgatt caatatctct gtagataagg tttcttcttt taataagatg ttgattaaaa
      421 tatcctcttg ccctgtgtat tcgattcttt gagtagcttc tttatcaatg atcaccacgt
      481 cactttcttc catctctatc ctttctccat taataatttg taaacatgta ccgctataca
      541 tataattgat ttcaacaaaa tcatgtgtat gctcgggaac ttccgcatat cgacggtgtt
      601 tactgatatg aatatttccc tgttgaaata gatcccctcg gtggattttt ggcggtgtct
      661 gaagatggta agctaagttt aaatcattga tattcattcc ttgttcacgc tgcagagttt
      721 cgattgattc ttcgttacgc aaaaaaaatg tctaattcag ctagttccaa ttgactccct
      781 cctttttgta agcggtttct ttcgcttatt agttcacgct gtactgctat tatatgcaaa
      841 gctatttttg gtttcataaa gactttctat ggttgggaca tgaacttcct gtgattccgc
      901 tattgagaga ataaccactt catactatat ttttgataag cgcttacaaa aatgaaagga
      961 acatgcttat gaataaaaag atgatgaaat tttctctgct gtcagtctct cttattttga
     1021 cttctgctgg tgcagtcacg tctgcaattc ccagtatgtc agctacttat gccacgcgtt
     1081 ctttatctgc tgttgaatta ttaacgactg ctccctcttt tatggtcact atttttgttt
     1141 tgattagtag ttttattgct aggaaaatcg gacaaaaaca aactgtgatc atcggattag
     1201 tgattgcatc gatttgtggt atcctccccc tcttctcaca aagttacagt attgtcttag
     1261 cttcaagagc tggactaggt attggctttg gattgatcaa ttctttggca gtcagcatga
     1321 ttgctgcctt ttatcacgga gatgaacgag cctctatgat tggttttcaa agtgcatttc
     1381 aaggatttgg tgcagcttta atgacatttg ttgcaggaca gctagtacag ttcggttggc
     1441 aatatacctt tttagtctac gccatcagct tacctattct tgtcttgttt gttttgtttg
     1501 ttcctacacc tgaaacaact gaacttcaat cgcatggcac agccccaaca agacaagtaa
     1561 tcaaaacgaa tcctaaaatg attagttatg gtatttttct cttgctggtc atcgtggtat
     1621 ataatgctgt atcgattaaa ttagccacag tcctgacgac ttattctcta ggaaacgaaa
     1681 ctaatgcagc gaatatctta acaatcatgc aattggcaag tatggctgcc ggagtttgtt
     1741 ttggctggct tcagacaaaa gcacatcgct atatgcttac actttcttta ctactgatgg
     1801 gaatcggctt tgtattgatt gccactgtga taaacctgta tgtgattggg tttggtgcga
     1861 tcctaacagg aatttctttt tcgttattca ttccttactt attcaaccaa gtaagtatcc
     1921 aagcgcccaa gtacgcggag agtttcaata cttcaatctt attggttggc gctaatttag
     1981 gtactttcat tgcaccatat ggtttgctct ttctctcttt tgcttctctt gggagtaaaa
     2041 cacttgctgt tttcgtcaat ggcagtatct tgctgttgat tagcgcgctt attagccttc
     2101 tagtagtaca caggacgaat agaaagacag tgataagagt agatgaagaa ataaaagaac
     2161 aattgacaac aaaataaatt tgatggaggt cgtgtaaaat gacaaatgaa aaacataaag
     2221 atagatggct ttggtatcct ggagactttg agatccgcca tggactgcta caaaatttcc
     2281 aacgggaaga acgaggtttt gactggccag cgtactggta catggatgat tgccatcgga
     2341 acgtcaagtt taagagatat tactttcttg atcaaccttc catgtttaaa gtgacgatcc
     2401 aaggagttgg gtatgtagaa attaatggac aaaaacatcc atgtggaaaa tggctgactt
     2461 gtccagctgg taaagcaaaa attcgtatct ttgtaggaca tacatcaggg ctaccagcga
     2521 tgtttatcga gggagacgaa gtaaaatcag atattggctg gacagcaagt aattttattg
     2581 aagaatatcc agctggctgg agtccgcttt atgtcgatat cgcaaaagat ccaaatcaaa
     2641 tctattatca aaaagaatgc gtccatccag tcactgaaac ggagattggc ggaggaatct
     2701 tattcgattt tgggcgagca gtgaatggct gtgtagaact aatatctctg gatgaccata
     2761 tcgaatctat cactctatgt tatggagagt ccgatgtaga agctatggat atcgattatt
     2821 gctactataa gcaaatagga atgacaagaa aaatgaaagc tcgtaaacgc gcttttcgct
     2881 atttgtttat accagaggtt tctactggaa tggttcaact tcaagttttt catgaatttt
     2941 tatctctttc gaaacaagct gctttttcaa gcgattcttc tttactgaat cagatctggt
     3001 cagtatcaga agaagccttc aagttatgta gtgatttgtt ttttatcgac ggtatcaaac
     3061 gagatcgctg gatttggtct ggagatgcgt atcaatcatt catgatcaat caatatcttt
     3121 tttttgacga ggaaatcaat aaacgaacga tattagcatt aagaggacaa aatgaaatca
     3181 agcagcatat gaatactatc gttgactatt cgatcttgtg gttgattggt atcgaaaacc
     3241 actatatgat gacgaaagac tatgaatttt tacgcatcgt ctaccctaag atgcaatcat
     3301 taatgagata cttgatggag caaacgaatg aattagggtt tatttatggg agagcaaaag
     3361 attggatatt tattgattgg tctgaaatgg ataaagaagg aacgcttgct gcagaacaag
     3421 tcttattact gaaagcctat cattccatga tcacttgcgg agatatctta gctgaaacat
     3481 atggagagaa cgaaaattat catactcatc tctatcaaga aaaatatcaa ctattgaaac
     3541 aaaacctatt tgactacttt tgggatgaag aacttggcgc ttttatcgat tgctatgaat
     3601 ctgggagaag aaatgtgacg cgtcatgcga atattttcgc tgttttattc gattttgcta
     3661 acaaagacca acagcaatcg atcttaaaga atgtcttatt gaatgacact atcacacaga
     3721 ttactactcc atatttcaaa ttctatgaac aagatgcttt atgcaaactc ggacagacga
     3781 atatcgtcta ccaaacgatt cttgattatt ggggagggat gttatctcat gatgcggtaa
     3841 ctttctggga agaatacgac cctactcaaa caggaaatga aaagtacagt atgtatggag
     3901 atccttatgg aaaaagctta tgccatgcat ggggagcaag cccaatctat cttttaggac
     3961 gctattttat cggtttgtat ccaacaaagc caggttatga agaatttatg atacaaccta
     4021 atctagattc atttgaaaca ctgaattgta cgctacctat caaagatgga aagatttcgc
     4081 ttgaaaaaac aaaagataag atcgtgatta cttctgatag aacaggcgga acagtgaaaa
     4141 tgaatggtaa aagttatcct cttctagcaa aacagccact tgtcttaaca agctattcaa
     4201 aaataaatgc ataaaacaaa aaactgcttg cgacatatca gaagacttta aatcttcttc
     4261 tgatattgtc caagcaattt ttattttgaa gtttgcttaa ttctgttctt tagaaacaat
     4321 gtttgttctt tttccggcaa aaagaaagct agataatacg acaagtaaaa agaaagcgat
     4381 gattccaact gtcaaggcta tttgaccgta ttctcctagt ttcccaagta caagtcctgg
     4441 tactagataa tcagtatcgg caaatgtaga agaagcgatt ttaagatctc ctaatactgg
     4501 aaggataaat aatggcagcc aactaatcaa taagctattg aaagcagaac ctaataccgc
     4561 ccctcttcta ccgccagtcg agttgccgaa gacaccagaa gcagctccgc agaagaaatg
     4621 tcctactacc ccaggaatga taactgtagt ttgcaagcct atcatagcaa acatggatag
     4681 tgttcctaca aagaagctaa caaaaaagcc aattaataca gcattaggtg catatggaaa
     4741 aacgatagga acatctaatg ctggtttaga atcgggtacg agttttttag caatcccttg
     4801 gaatgctgga acgatttctg ccaacaccat tcgaacccct tgaaggacga taacaaaccc
     4861 agctgtgaat gttccagctt ggatcagtgc aaagattaca gcgttgtctc cattgcttag
     4921 ttctctttca acaaatccag gaccagctaa caaagcaagt gcgacatata cgatcgacat
     4981 caagagcatg atcgatacag tagaatctcg caaaaagctg aaacttttag gaatctttat
     5041 gtcctctgta gaacggtctt tgtttccaaa aaactttccg atccatccac tagaagcata
     5101 accgatattt ccggtatgcc ctaacgcaac ttgattgttt ccagtgatgt tcttcataaa
     5161 cggttgacaa agagcaggcg taatagttaa tagtgttcct tcgaagaccc cgcctaataa
     5221 gatcatccac aaatttgcag taaaccccgc gcttactaaa atgactgctg ttagacaaga
     5281 aacataaagc attgcttgac ctgtaagaaa aatgaattta tatcttgtga atctagcaag
     5341 taaaatattc acgatcatcc ctactagcat gatcaaagca gtaggtgtac cgtattgttg
     5401 caaagcaatc gccactactg cttcattgct aggaactact ccttgagtat gcagagctgt
     5461 ttggaacatc gaagcaaaag gggttagtga attactgatt aaaccagcac cgccgatcag
     5521 tactaggaat ccgacaaatg ttttgatagt tccttggatc acttctgaaa atttcttctt
     5581 ctgaagaacg agaccaagca gtgcaatcaa agaaacgagg atggagggct gactgacgat
     5641 tgacacaaac aaattcaaca atttatccat tgatagttcc tcccttgaat catttttatt
     5701 cttttgacaa gatcttcgtt actttttctt ttacttcttc gctgtcaatg atcgattgca
     5761 aaggtacgag cttttcatca ggtatagaag ttagcgtatc tttcaaagaa tgctcaatga
     5821 agaaataatc agccatatcg ctagatacac ttccaacatc cgtatgactt acttcaaact
     5881 cgtccacatc tgctccaaga tctgcaagtg cttgtttgat gttcatctct accataaaac
     5941 ttgttcctaa tccactttga catactgcaa aaattttcat atcattcgtc tccttttctt
     6001 atgatcgcta atatttcttc ttttgttgat gcgtgtaata aatcttgaag attttgttta
     6061 ttagaaagta gctttgttaa atttgccaaa gcccgaagat gtgcctcatt atcgatagca
     6121 gctaaacaga tgaacaaact aatgggatgg gctggattat caagcagatc cacaggttgt
     6181 tgaagtttca gcaaagacat ccctacttcg tttacgccat cttctggacg agcatgaggt
     6241 aatgcgatgt gatcgccaat atggataaac gcaccatact gctccacgcg ctcgatcata
     6301 gctttgatat aactttcttc tattttattt tctgataata gcggctgtgc ggcaaatgtg
     6361 attgcttctt gccagttttt tggttgatcc gataattgga tcatttcttc tgttaataat
     6421 tcagatagca taggacgttg atcctccttc ttttctaaaa gtttgttcat tttacgattt
     6481 aatacttgat agagcttttc ttcgtctacg ccttctttta attcgatata aggcagtagc
     6541 gccgacaaca attcttgggc agaaggcatt gaaaatcctg gtatcagcca atctctttgt
     6601 acttgattga tcagctgatt tttttccaag tcagtcatga ttggatgcag aacatagaga
     6661 gactttttct tatcagtaag tgtgagaggt acagtagaaa aaataatatc gtaagaagtt
     6721 tcgtcaacct cgtgtaagcg gtcagctgaa ttagtctctg taaaaaagat catcgggaat
     6781 aattgctgaa gttcactttt taagatcaat gaagagctaa tgccacttgg acagacgatc
     6841 acggctctta tttttctatt tctttcttct gcgtccgctt ttctgatctc tccgccaaat
     6901 agtatagtga aaaaaccaat ctcttcttca gggatgctct tccctattcg tctttctaac
     6961 ggaagtaatg cttttcttgt catttcgtag atggaagcat attgatgctt aatttgatcg
     7021 atcatcacat taggtaaata aaaagaatag gaaatacgaa aataggcagg gaccaagtga
     7081 taaaagacat tcatcaataa ttcacgaaac gaagcaaatt ctacggccat caggaattcc
     7141 atgcgatgga tgacttctga agaacattcc agcaaaaaat caagtgcagg ctcctggaca
     7201 tctccttcaa cgatcgatgc taagcaaaga gagaaaaata taatctccga attttgatgt
     7261 tcggaatagc caaggccagt caatacttgt cgactaatgt cagtgactgc aagagaagcc
     7321 aacagttcag catcttttac cagttccatg gatgtttttc caattcgaat ggttgtcaaa
     7381 ctcaaataat aagctaattc atccattcga tttggtgcga atcgaagatc tgatagtgaa
     7441 atcgttttat ctagcaaatc tctaactttg gcgtaataat caatggaaag ggttgcggtt
     7501 aaatacgtga gcccaaatct tccagcaagt gactgcatca aacgagcagt gcgatttttt
     7561 gcttctcttc ttaatattgc ttctgaccct agcagcctaa aaccagtttt cctagtatac
     7621 gacagctgac aatcttccac ttctaattca tttcttatct ttttgatatc tgacaggact
     7681 gtccctttag aaacctgaag aaaatcttga aaatgaaaaa cagaaacaga cgttgcagtc
     7741 gaataaacca tcaaataaag aagatctctt cgctcttgtt cggtatacac agcttcaaat
     7801 cgagtaatat ccaaaaattt ttctgcccag tcgattgctc ggaatttctt tatacggata
     7861 cgggtatcat ccacatattc ggcaaaagga gttaattgtt tgatcgcctc gcttgtattt
     7921 acttcgatga tctctgctgc tttttctaga ctgatttctc ttggtgtttt caggagataa
     7981 ctaattttgt agttcacagc gctgttcacc tcctacttat attctagtat ggtagcgctt
     8041 ttaaatcgag gggtgtttgg tacaacttac agttggattt tttttacgac tgtaccgaac
     8101 gatttctatc gaatggatac agaaaaaaag caggtgacca aatgtctcct gcttttatga
     8161 attgatttcg tccgatcaat gatgatcatg tttatttttt gaggtcagtt cttcaaattt
     8221 ttctttgaca tcttcaaccg ctttttcgat atctttttca aagtgattgg gctcattagg
     8281 ctttgtttcc tcttctacag aatttgaacc agataaagct gtttctttat tttctgtttc
     8341 accagagttt gtgccagcta atgattcttc tttgtcttct gtttcaccag aattagttcc
     8401 tgctaaagcc acttcttctt ctgttttagg ggccattgaa cctcttaatc gtttgtgttt
     8461 ttcaccgtca taatttgtca tgttggttcc tcctagtagt tctatacttt ttcatcgtac
     8521 aataagacag tttatgcgtc aaaaaacaaa cccgattctt acaaatatgt catatttttc
     8581 cttctgattc ttgaatgctt ttgattcctt cttccaaaat tttcatttgt aagattgttt
     8641 cttcatcttt gatgactttt tctgcgccat taactaatgt ctcgtaaatc ccttcgtaga
     8701 cacggctgta atcaccaacc tcggaaatta ctttttcttc atgataactc ccttgttcat
     8761 ctatataggt caatacacca taatgttcgg gcaaatcgat accaaagtct tcatgatcag
     8821 gcatgtaaaa cattttcaga tgttcttctt gtcgatcttt tgtttgtttc acgaacatcc
     8881 ctttttttcc gtaaacgacg aaacttggac gttctttgat gcggaaataa ctggatttta
     8941 tcgaaatttt catatttcca tagaaaaaat ccagatcgaa gtaatcattc attcgtcctt
     9001 tccctaaaag ttgacgtaca tcatagctga tatcatctgg ttcgccgaaa taagaaagaa
     9061 cttgatctac tgtatgacaa gcatgtccat ataaatagct gttcgcagta gaaaattcat
     9121 gcgtattttc tggtacttcc ggtctgaaat aatcataatg catttctact tctaaaatat
     9181 cccctaattt tccgctttcg attacttttt ggactgtcaa gaagtcagaa tcgaaccgtc
     9241 tgttttggta acattggata aatagacctt tttcttttgc taatgcaaat aactcttctg
     9301 cttcctttga cgtttctgca aaaggtttct ctactagtac attcttacca tgttcgagtg
     9361 ccatttttcc atattcataa tgaaattgac taggtgtagt gatgacaact aattggatct
     9421 caggatcatc ccaaagatca gacagctgat cagtatattc gatgtcttcg aatgttttcc
     9481 acactggttc agatggggaa aaaatttttt tgatattgat tttgttcgta agttttcttg
     9541 aaaaaggcaa atgatagcga tttgtactct ttccattgcc aagataagcg attgtaagca
     9601 tgagtggtgc ctccttttat tgatgttttc agtatatcct agaaaagcgc agaaagtatt
     9661 tataatagct attctggtaa aaaagttcag aaaaacactt gttatcattc ggtttggtga
     9721 aattaatgtt caaagtaagg ataaaaatca aaaagatagt agcggaacca agtgtttatc
     9781 agtaagatag aatttggagg tcagatcatg cttattgaag aaaaactaac aaaacaagag
     9841 ttgtttacca cgacagagaa acggattgtc gattacatcc gtaggaatat cgaagctgca
     9901 gtctacatga cgattgaaga attagcaaaa gctacgtata cttcccattc agctatcatt
     9961 cgtttatgca aaaaaatggg attcagcgga tttaaagaat tccgtttcga gctggggcgg
    10021 gaagttcatc aattgattgc tcagttcaat caaacgattg atcctaattt tcctttttca
    10081 agtgaagata gcactcagca aattgcaaag aaaatggcgg atttatcgat ccagtcaata
    10141 aaaaaagcac aattgcagat tgaacatgaa gatttagatc aaatcggtaa agtattgaac
    10201 aaggcacagc gaatctttct ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc
    10261 cagaataaaa tggtcaagtt gaataagttt ttgatcatgg ctgaagaata cagtgattct
    10321 tcttggaatg cagccaattt gactagcgag gattgcgcca tttttattag ctacagcggt
    10381 cgcatccatc attatgaacg aataatgacc tatttgaagc atgtagaggc ccctaccttg
    10441 ctgattaccg gaaatcagca ttcagaaatg gccaaacagg ctgatatgtg tcttgttata
    10501 tcgcaggagg aatatgattt tgctaaagta gcgactttct cctctcaaat tgccttcgat
    10561 tacgtattaa atacgttatt ctcagtgata tattctcaaa attttgaaga gaatatccgc
    10621 aacttgaaaa acaaacaaga attgatgcaa aaaggccttc ttaaagaata tcggaaaact
    10681 taagaaaaga tcctcattta gtaataagaa agatagtgtt tgataggctc tctttgatat
    10741 catcataaaa acaggctgaa gttttctttt tcaaacctca gcctgtttgt ttacggaacg
    10801 attcgaatag atatattata gaaatgagga atagctaact tctgtttctt attttgaacg
    10861 attgacgaaa aagatatata agaaggagga aaatcagtat acatgcagct aacaatccat
    10921 acaggaaaaa tcgtttgttc tcggatactt tctgtacttc tttttgaact tcttttgcat
    10981 gataaggaac ccggtgccct ctgacaagta agcgatgact gttgaccata taaggtgtac
    11041 aagttaggag tgtcacgaaa tcctgccctt tttcgattct cagatctttt gtatcagtcg
    11101 gttctaccgt cttgatctga tcgacttgat aagcgagtgt ccgtccattg atttcgatat
    11161 agaaagaatc acctaaagtc agtttcggta agtctgtgaa caactttgct tgaggcagtc
    11221 ctcgatggct ggaaataacg gcatgtgtat tttctccccc cgtaggataa gacgttcctt
    11281 ccaataatga ggcacctttt tctagcagca cagtattcgt ttcatcaaaa attggcaaat
    11341 ggacattgat ctcagggatc gtcaaaatac cgatcgtatg tttctcaaaa taggttctat
    11401 cctttttcgc aggttctttt ttcttagtaa agggatctgc acctggattt ccaccttctt
    11461 ttgctaattg ctggtttttc tttgtcatgt tttcttggat tttagccatg acttcttcat
    11521 tttctttgac cgcttgttgc tgataatgag agatgatctg ctgatccaga taattattca
    11581 gcgcgtcgct aacaaatgga taagctagta tccctattcc tgagagaagc aagaaaagca
    11641 tcagcagatc tagaagtttc cttttctttg gtttcatttg tgttctcctt gtgatgactg
    11701 tttgataata aaagaaaaag gagcatttgc gtaatccgct gctccttttt ccaattcttg
    11761 caaaaagact aaatctgact gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga
    11821 aggactactc ctgcaccgat atacacatag attcccttac cgcctgttga aggaagtgta
    11881 cctttgtgtt tatttggtat tttttcagga gaaatcaact gtcctgctgt tacataagat
    11941 tgttcatcga tagtaaatgc tacacggttt gttaatggaa catatttttc tggcgctttc
    12001 gtttcttcca gatagtacgt gccatatttt agacctgtca catcgattaa accgttactt
    12061 gtggttgtaa aaaccgttgc tgattctttc gccgataccc agctgacggc ttttgtggat
    12121 gggtcgatcg ataaatattt cgctgtgtca ctatcttgat cacgaacgac gaattctgct
    12181 ccagcaagtg tttggtctga tgtaacgtca ccatctactt taacaaatcg tttgccccca
    12241 gtaacgacat cgactgacgg tggtgtttga tcatttgtat gcccgttatc gacatttgct
    12301 tggttagaaa atcctttggt tggatctgct ttttcgttca aatgcatata gtaaacgaat
    12361 ttcaatgtac cgccaggagt taatgaagga atataattcg gatcaactga aaccgtgaat
    12421 ccgtctgttt gctcagtgac agaataattt actgggtcga tttctttatt tccatcatat
    12481 aaggcataag catacgttcc tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta
    12541 tcgataagtt tgaatgttgt gtacttgttt tgcgttcctt ctttatcagc gatacctaat
    12601 gggatattga cagaaatctc atattgaatt ttttcaccga ttgcgacatc ttttccattc
    12661 aattgaggtg ttgttttatc aactttagac gtatcatttt tgatggtctt ttctactggt
    12721 gtttggctat ttgccaggat ctcaaaaggc gtgattgttt gcttttcaat catttccgca
    12781 ttatcaggag cttttacttc ttctaaatta tattttccaa cttctaaatg attaacgatc
    12841 aactggcctt tttcaataga tgcctcggca aagtcattgt tgccgatgtc ataagaatga
    12901 ccagtaatga aatgtttggc tttggtttga tcagttgtcc aagtgtacaa tccatctgtg
    12961 acactttgga tgtatttttt gacgcttggt gttccttctt ctttagaaat aataaattct
    13021 gctccattta gtgcttcgtt ttcggcagta ccgatttttg taactttcaa cgttccatca
    13081 ttaccgactg tatttttagg gtagagatgg atagtatcta gttcttctgt cccgtattta
    13141 taagagccat ctgcttgttt gatcatctca tatacaggga aagctaaaac catgtttgcg
    13201 gcagctgaca ctccgtcttt tggttcttct ttgatcgtgt agactgcatc tttcccattt
    13261 tgttttttag gtaaagataa agtgacattt ccatcagcat ctgtcgttcc tgaagcaact
    13321 ggtgtaccag gagtcaaaga ctggactgct tgttttgctg catccacgga cgctccttta
    13381 tctcgttgcg cataaaattc ttgagtgacg ttataaactg aaaatgaaat atcggctaat
    13441 ccttggtatt gatcgaattc gctcatttct ttcccgctgt tttggattaa aggatcgggt
    13501 aaatcagtca ttttcttttt atgaaggatg acttgagctg ctgtctcctc tgcaaacact
    13561 ttttttgcgc cgaagctgtt tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata
    13621 acgtttattt ttttatgatt tttcattttt tcactcctgt tctttttttg ataaacaacc
    13681 agattgcaaa gcttgcgaat accataccaa caacgcctaa aagtgtattt ttcgtttcgt
    13741 ttgtttttgg aagaaatgga cggctatttt cttttttatt gactactttc aatatactgt
    13801 ccgcctcttt atcctcagaa agaaaagttg aaatggtaaa cggaatttct gtttcactta
    13861 aaatataacc agaaggtgct tcgatctctt ttaatcgata acttcctgtt ttcaacccgg
    13921 aaattgaaaa agcgccattt ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg
    13981 tccacttgta tccgttggct gtttgctgga ggtaatttcc tgctttgttt ttaataagga
    14041 atactgcgcc agctaatcgt ttatctggtt tatttgtctc gattttgaca aattgcttac
    14101 tccctgtata tacagagact ttctttgtac tgacaccaaa accaaaatcc agtgttccgt
    14161 tgttgttgat ttccttgttc gcctgtgctg tactgcttag ctgcatctga taagaaatgg
    14221 tcagtttttt tccagcatat ttttccaacg aagggatcga aaataaaaca tcaaatccgt
    14281 gaggtttctt gtgtatgaca aagccttctg tcagcttttc tccattcgat gaaatcgtta
    14341 aactattagg taaaaacgtt aatgcttcat ccgcactatc tgacaattcg aacttttgat
    14401 agtcaaggat atttacagga attgtcgtat gcaaggaata agtgattttt tcaccaatcg
    14461 taaaatcatt tctaggctct tctaatacct tctcaaaagg tggatcaggg tagtcgtttt
    14521 cttcattttt aggatacaaa tggatagttg aaagtttttg attgttttgt ccatgtacag
    14581 gaagaacaac taccatattc tctgcctttt ctttgacgac ttctggtact ttggattcaa
    14641 tgaaaagata cgctttatct cttttttctt gatctttagc ggccagagaa aaagaagcga
    14701 taccatcttc gttgttaaga gttgtagttg ttgcttctgc ggtaaacata ccggaagacg
    14761 caccgttttt tgcgatctct gcttgtgctt cttctaccgt tttgcccttt tcccgtagat
    14821 ggtaaaaaga atctgtgaca tcataaactt ggaatgtgac accatttaat cctcgatacg
    14881 tttgtaataa agctttttct tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg
    14941 ggaaaagcaa tttgtgtaaa gtaatctgaa ccatttctgt ttctgttgct acctgagtaa
    15001 aagctggttt aaatagtaac aagaagagac acatactaag ccaaccaagt tttttcatat
    15061 ttagctgccc cctctttctt ttttcaaaaa aagataccaa atagaaaacg cacaaccaat
    15121 catccctact agatagattc ctaaacggcc aattcctccc gtttcaggta atggtacttt
    15181 tgcctgattc gtgatgtcta aagaaatctg gttgtttttg gctcctggtg acagaacaga
    15241 ttcatgatct tgtccatcca cttgaattga cccatcttcg tgtataacta tagtaactgg
    15301 ctcttttaga ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat aagttccagg
    15361 ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg attctaagtc
    15421 catttctggt ccctgcagtc tgaattttgc tccttttaat gtctgtccgt tgtcttcttt
    15481 tttgtgtacc gttagatcaa acgcttttag tcgattttga tgagtgagcg tccatacatt
    15541 atttgtcatc tcggtatttt ttcccgtgta aatgattttt ccatatttat cgatttggaa
    15601 ttcataattt cccggcgaag tatcatatcc ggcaggacca gctgtttcaa cgactcgata
    15661 tttaccaggt gtgagtgaat caaaactggc aagacctttt tcattcgttg tttggctgtc
    15721 aattggctga taagtaccat ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag
    15781 atttatctct ttgtccgtcc cttgcatcgc atacttacgt actgctacag gaacttcaac
    15841 aaaaggattt tcaatagtca actggatcgt atcatcggaa gtagtgactg tttttccatc
    15901 aatggttacc gtaccatcag aagcgatctt gatttcccaa gtagtctttt tgcttaatcc
    15961 atgcccttct ggagcttttg tttctgtcag cgtataggtc atttcttttt gcaatttgac
    16021 attttctgga agagaatagg ttccgtcgcc atgatctgtt agtaaagtat caatagaatc
    16081 tcctgttaat ttgaaaacag cgccaataag atccttttca cctgtagagg aatttttcgt
    16141 tattttcaag tttaacggtg tgaattgttt agtatttttc catgtcattg catctatttg
    16201 ttgagaatcg tatcctggta caggtaattc tttgattgtt tggtaactga atgcttgacc
    16261 ttgattattg tattgaggta gtgataatat ctcttgataa ctttctccgc tatttgcaga
    16321 taatttgtca atgtctgcac gttcccacgt atttgttgta tctttagctg gtttaatgat
    16381 tcgaatatat ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa
    16441 agtaacttga tctggacgat cagctagatt gttgtcaaat tcttcccata actttttgat
    16501 gtgaagactg actccgggag ctttagcaga tggtataccg aattcagcta attcattggt
    16561 atcaatgttt ggctggaaag ttgtcctgcc gttcatttga taccagaaat ttggatggaa
    16621 gtcctcattt tctgtttgga ttctcacttg gtaatggatt tggatttctt ggtcttttcc
    16681 taaatagatc tgattgctct tgatggtatt tccttctatg gaaatagatg gagtgaccgt
    16741 tgtaggactt gtccccacac tcttgactga aagtgtacca ggctgataaa tgaatggttc
    16801 tgcgattgga tcatttattt gtccattgct tacagttgct gagatctgta cagctttttt
    16861 ggctaggtat tcagagatat ctgttgcatg atcagctgat tcatagtaaa gatcgccttt
    16921 ttcatctgat gaaaccattt gtcgcatacg agactctact tctgcttttg agagaccagc
    16981 tgccggatcg ctttgaagtt ggatgccaag accatgtatt tcgattcctc gttctttgag
    17041 tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg attcttctgg atagattgtt
    17101 ttggtcaggt gcatcataga ttcttgagat tagagaagta tttcccggcc gatcctgcgt
    17161 attagaaaac tgagttccgt aataattgct gcttgattgt gcgtgtaccc gctgtacttt
    17221 ataggaaaat gttggtacac catccgtcag caaaacgatc acttttttat gtccatttgg
    17281 aacggatagc atgcttcctg catctcttag tgctttttgt gtaaaagtac caccatttgt
    17341 ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa tcaaatgaac ccatctgtac
    17401 tgcaccgtta ctgtagctat atccttcgct tgagtagccg acatatccca tattgatttt
    17461 gtctgtgata ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa tctttacttc
    17521 accgatccga ttattgtcgt tcatacttcc tgaccagtct acgaccaata cgagatcaag
    17581 aggcgtgata tctttttgta catttccacg agcattcaaa taaacatcaa acaatccggg
    17641 tgtacttgtt tcctttgcat actttcgtat cgcatagtct gcttgattac cggttcctcc
    17701 atattcgata tacgaatggg tccgatcatt aggatctcca ttccaagaat ttataccatc
    17761 ccattgattt gttccgtttt tattgccttg atgatcaaga acatttgtat ttcctgtagg
    17821 ttgccaactg gctttaggat aagtgccctg atcatctgtt gtatattctg taggaatcgt
    17881 ttgattagat ggcacactca cagctggcaa ggataatgta ggcaatgtaa atgggctgac
    17941 agaattttcg ttttcttttg atgattcttc ttctatttcg tctggctttg gtactgacac
    18001 atctgtatct ttcttatttt ctgtctcatc aataatgtca tattctttat tatcgacgat
    18061 cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt
    18121 ttgtttagtg tttgatgaga tttccaacgt aattttcccg ctatttacct gataagcata
    18181 gccatctcct tcttttcttt ccacatcact taagtaattt tcgactagac caatttcttt
    18241 ttctatcttt tcagcactgg tgttattcgc atttagcttg atgttcagtg ctttttttcc
    18301 agactgatcc gaaatgactt cagctgaaat tgtttctaaa actgttggat catctgtagt
    18361 gtctgctatt gcagataatg gcacaaagtt tgataaactc aatattatca gcatgaaaat
    18421 aactttcagt ttcttccctg tggtcgtcat ttttcccctc ctttttcttt ttttctgttt
    18481 tgacgtgaat agtgaaaatt attaaaataa acaggcgtta cataatttta tttattttaa
    18541 tatgataatt attatacgaa gggcgctaaa gggaatcaca ccaattttca taccaattgg
    18601 tttcatttat gaagttgatt ccttcgatta atgagcgttt cattgaaaga aaagggttga
    18661 gggagaaaaa catatgatca ggttgttgga acgaaactat agaaagatgg aaaaaatcat
    18721 tcgaattctg tccatttctg acaagtggat gactaaaaaa gaacttgcca aacgaattgg
    18781 tagttcagaa tctacatttg taaggtataa tcgaagagat caaaatccgc tggggaacgg
    18841 atgtaacgat tcaatcttca caaaaattgg ggtatcgtct tgaacatttg aatgtaccag
    18901 cttatttaaa tgttctgatg gaaatgtcgc aaagttctac aaacagtcgt ttattaaatg
    18961 agattattgt caatccgggt aaaacgattg agtattattg tgaaaaaatt tccatcagcc
    19021 ggtcttcttt tacacgtaag ttgaaacaat gcaatcaaat tttagaaagc tatttattaa
    19081 aagtgatcgt tgaccaaggt tttcagttaa ctagccttaa aagtgagctc cagttgcgta
    19141 tctttgttac ctttttcttt ttaagttact acggaagacg cacgttgcca tttccaatcg
    19201 aaaaaaagaa gatcaaagaa attatggtca agaataattg tcagatgaat ttgtttcagg
    19261 aagaaagccc atatgagcaa tctttcttta ttatgtattt tgttgtgcat ctcatgagag
    19321 aatcccaagg aactccattt gaatggaaag ctggagactc ttatgtacag ttgataaata
    19381 tcgataaaaa agacttctta atcttgaaaa atctcttagg aaaattgaag tacagcagct
    19441 atataaaagt agtgaactat tttgtcaata cgattttttc agaattttcg gagaaagaaa
    19501 aaaatgaaat cagtgagcag atacgaaaaa atatgtccta ctgttcgttt tcatccgctg
    19561 attgcaaaga aaaaaacgaa gaatatgttg ttcaattatt aacgcaaatt tattttttct
    19621 cccgtatttt cccatacgat acgactcatt tgacgatccg tgcaacttac tttgcacagc
    19681 agatgcaaca ccgccagata cgtctatatg ctggatttcg tgcagaactt caattttatt
    19741 ccactctact gaatcagaat ctgatttatt atttatctag ctatatcttt tggatgctta
    19801 tcggtaatcc tgaaattcac cattatactt ctgataaaaa aatactgatt atcagcgact
    19861 taagtcttcg acatagtcaa tatattgaag aatatatcag tgatattctt gctgtgcata
    19921 aaattcactc tgagacaact gctattactg aagatcagct gtcgaaatac aatcttctcg
    19981 agtatgatct gatcgttacg aatcaaccca ttttgaatgc ccatgttcct catatattga
    20041 ttgatgatag tgtgtcattc gcaaatgaag aagaactcat ccgcttattc gagttatgaa
    20101 taatcaaaaa acagctatgt ataaaccacc ctgataaaaa tttcaggaca aatacacata
    20161 gctgtttctt tttattgtat tccttcagca atccaacgag atttatagcg aaagtctttt
    20221 aacaactctt cttttgtcca attatttgca atgagatccg tcaaagtgtt cattacgagg
    20281 gtcatggaaa agtataggaa actaactgct tcttccgacg attgaatgga gaaatgattg
    20341 attcttaata attccagcca tttttcgttc attggagaat cagccgatag ttctctacgt
    20401 ttggttgtct gctgatcatt tcgctgggtt aacaagtata acctttgcca atagatactt
    20461 tcgtgatcta agtcgcagca ccactctaaa tatttttcga tccccaaaaa gaaatgttgt
    20521 ttgttttcgc taatgaattt taagatatct tgatggacta tgtgagaact gcttttgatc
    20581 gtatacaaat aagcatcttc caaatcttga aaatatttat aaaaagcacc gcgcgacatc
    20641 cccatcttgt caacgatttc tgagactttg acttggctga tgtgctgtga gcaaaatatc
    20701 tccagtaata ggtcatcgat tctctgtttc tttgcgttcg ataaattgaa aaaagtttct
    20761 tttggcatcc acatctctcc tttatctctg attatacgtg gatagccctt cattgtcatg
    20821 aaggattcat tccttctttt tctcttcttc tgtcaagtac ccatcttcca ttcgataaac
    20881 gcgatcactc cattgaatca tacggacatc atgagtaacc ataatagtcg ctttttgttt
    20941 ctcatgagct tctttaacta gtaatttgac gacttcatat gcatgatctg tatccaaact
    21001 agctgttggt tcgtctgcta agatcaagct tggctcatta tataaagcgc gagcaattgc
    21061 cactctctgt ctttctccac cagaaaggtc tttaggataa ctatttttaa gtgactggat
    21121 atctaaagat tgcagcaagg aatcgatttt tgttgaatct tctttttttg aagtaacttg
    21181 gtcaactagt tcaaattgtt cctttaccgt taaaaaagga atcaaattag agctttggag
    21241 aataaatcct atttctttaa agcgtaaatc tgctcgtttt ttttctggaa gctcagttag
    21301 tgagtgaccg ttgatcacta tttctccgga agtcggtgtt tgcaatcctc ctgaaatggt
    21361 aaggaaagta cttttacctg aaccagaagg accaatgatg ctgacgaatt ctccttcctt
    21421 tacataaaaa tcaattgctt tcaaagctga gacctttgta tgaccagaac caaatgtttt
    21481 ggtaactttt ttcatcgtta atattttttt catttttatt aacctcctat tgctgtaatt
    21541 gggtctactt tggttatcgt acgtatcgaa aacaatccgc ctagcattgc gacaatgatc
    21601 aaaatcccac tatatagcaa ccattgtgac caaaaaacag caaaaggcat cgcgcttggt
    21661 aaaatcagac tagtcaaata agcaaacaaa agagcaagca gaacgcctac caccccaaca
    21721 ataaaggact gtgctactaa agatttagcg atgaagaaat ttttgatccc ttgtgctttc
    21781 attactccaa aaatagcagt cttctgaagt gtcatcacgt acatgaaaat accgaccacg
    21841 gcagcaacca ctaagaacaa aaagtatatc atcgcattta aggtcatatt ttgggcagaa
    21901 tagccaggaa tactttcaat gaatgtccca atagttaata actgtagacc cttcgatgct
    21961 ttttctttcg aaattttagc tgaatccttt attacgatcc cattgatcgg ctgttcttct
    22021 tcagaagcaa acggctgttg tccatacttc aaagcagtcc acgtatctag ggacgcatag
    22081 atgacgggag aaactgtgta ataagtttca ggaaagatac cgacaatcgt caaaggatcg
    22141 tcgtaactgc caattttgat cgtatctcca attttatagc ctgaatcagc cagattttga
    22201 gagatgatga tttcattctt tgttttaaat tcatgccctt tcgtgagttt tggtaataaa
    22261 aatgcttctt tcgttgtgcc gaaaactgta acattttctt tgttttttcc tttgattgct
    22321 ccgctgtaca atccgattgg tgctttctct cctccttcaa tgtatttcag atctcctctt
    22381 gttaattgtg aagctgcaaa tacttgattt gcatcctccg acaaaacaat ctcttgtgcg
    22441 tcccaatcat cgattgcttt tttaaactct tctgaaagtc cattcgctag tcctgacaac
    22501 atgaatacga catacgcaat caagaacata ataccgacga tcaagccata tcgtaattta
    22561 gaatagcgaa tttctttgat agctagaaac atttgttttt cctccctatg atagattgat
    22621 agtgacaaaa tgtcacttgt tgaatagtgt aactctaaag tgacaatttg tcacttgtgt
    22681 tgtttttttc ttatcaataa attgtctaag acatctttcg ataaaacctt aacatgagtc
    22741 ataaaaaatt tatttaagcc ttatttatgc tttccatgat aaactaaagt gaccaaatca
    22801 atgaggtggg aaaatgatta aactattagt agtagaagac gataaagtat tatccaataa
    22861 tataaaagaa atactcgctg caatcggcga gataacacaa gtttatgatg gtagtgaagg
    22921 actgttcgag ttggaaagtg gagtttatga tcttgcaatc ttggatttga tgatgccgga
    22981 aatgaatgga tatgaagtgt tagcaaaaat acggaaagaa aaaatacaga ctccagtatt
    23041 gattttgact gcaaaagata gtttggaaga taagattgaa ggatttgaac gaggtgcaga
    23101 tgattatcta acaaaacctt tctatcgaga agaactgatc atgcgcgtga aagcattatt
    23161 gaaacgctcg ctaggtctct ttgatgaaaa tgttctgaag tataaggata tcagttgtga
    23221 tttaaaaagc aatgaagtct tgtataaaga acaagttttg cccatacaag gaaaagagtt
    23281 tgaattgctc gtttattttt tacaaaataa gggaatcatt ttgacaaaag agcagatatt
    23341 tgaccggatc tggggatttg attctgatac gacgattaca gttattgaag tttatatgag
    23401 caacttacga aaacatctgc gaagtactgg attggataaa gaaatcaaaa ccttacgtaa
    23461 tgtcggttat atactgcaaa gggtgtgatg caatgaatgg agatattaca cgaaagcaac
    23521 gtagtcgctt ttttgcgaca aatgtagcag catttgcttt tatctttttt cttttgggat
    23581 tcattacttt acagatatta aaccaatctg catacagaga aacagatctg tcattagaga
    23641 aaataaattt tgattctcga acagtacaat tagagataca aagatatcag caaggcgatc
    23701 cctttttaaa cccgactcaa acggaacaaa acagaacaaa catggaacag ccattaatgg
    23761 aaccatcaaa tgaccgtttt aacactcaag tgatcttgtg gtctaaaaat ggtgagatat
    23821 tgaataaagc agctattggt gggcgtttca accaaatcaa agaattaaaa atggatacga
    23881 aaaatctgaa taccatccaa aatattgaat taaatgaaga cagtggctca tctgccctct
    23941 ctttccgttc gattacgaaa aaagcggaga cgaatcaaga tggcgttgcc tacatccaaa
    24001 ttttagaaaa tacaaatcaa atcaagaatt cattagcgac ttttcgcacg attatgattt
    24061 tgtgtatgat tgttttttgg attctctcta ttggtatcag ttattacttg tctagtctaa
    24121 gcatgcgccc tattattctt tcttggagaa gacaaaaaga atttgttgag aatgcttctc
    24181 atgaattgcg cacaccgtta acgattattc aaaatagttt ggaacatctt tttaccaagc
    24241 ctaatcatac tattattgaa gaatccgaaa gcatcgctca agcattgtca gagacgaggc
    24301 gcctcactgg attgacttcg gatttgctga caatcgctcg gaatgattct gatcagcagc
    24361 tgctttcaaa gcaaatgatc aatacacaag aatatatcga acaacttgtt aaaccatttc
    24421 aagagatggc catcatagat ggaaaagagt ttattctcca taacgatgca acattagaag
    24481 taccaattga tgaaaaaaaa atccatcaag ttttggttat tttgctggat aatgcattaa
    24541 aatatacgaa tccagaagat aagatcattt tggattcatc tgttaaccat aaagaatggc
    24601 tgattcacgt aaagaatact ggcccttcta tcagtgaaga agatcaagca aggatttttg
    24661 atcgatttta tcgggaagat caatctcggg cgaaagagac tggcggatac gggcttggct
    24721 tggcaattgc aaaacaggta atcgaacagc atcatggaca gatttctgta tccaattatc
    24781 ttccacaagg agtcgatttt aaagtccaat tgccgttgaa gcctaaaaaa taatgcttat
    24841 ccctaaaaaa gggctgtgac aaaaatcttg tcacagccct tttttgttgc ga
//
LOCUS       DACEOE010000024        24305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEOE010000024 DACEOE010000000
VERSION     DACEOE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.025"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(7..1375)
                     /gene="nhaC"
                     /locus_tag="IW774_000833"
                     /pseudo
     CDS             complement(7..1375)
                     /gene="nhaC"
                     /locus_tag="IW774_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
     gene            complement(1484..2905)
                     /locus_tag="IW774_000834"
     CDS             complement(1484..2905)
                     /locus_tag="IW774_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1153199.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3148..5025)
                     /gene="tdc"
                     /locus_tag="IW774_000835"
     CDS             complement(3148..5025)
                     /gene="tdc"
                     /locus_tag="IW774_000835"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1153200.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5316..6572)
                     /locus_tag="IW774_000836"
     CDS             complement(5316..6572)
                     /locus_tag="IW774_000836"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1153201.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7152..8453
                     /locus_tag="IW774_000837"
     CDS             7152..8453
                     /locus_tag="IW774_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1153202.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8522..9121)
                     /locus_tag="IW774_000838"
     CDS             complement(8522..9121)
                     /locus_tag="IW774_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1153203.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9375..10256
                     /locus_tag="IW774_000839"
     CDS             9375..10256
                     /locus_tag="IW774_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153204.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10269..11879
                     /locus_tag="IW774_000840"
     CDS             10269..11879
                     /locus_tag="IW774_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ1153205.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(11956..12615)
                     /locus_tag="IW774_000841"
     CDS             complement(11956..12615)
                     /locus_tag="IW774_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1153206.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12898..13563)
                     /locus_tag="IW774_000842"
     CDS             complement(12898..13563)
                     /locus_tag="IW774_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153207.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13710..14315)
                     /gene="dhaL"
                     /locus_tag="IW774_000843"
     CDS             complement(13710..14315)
                     /gene="dhaL"
                     /locus_tag="IW774_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1153208.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEPLKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14343..15326)
                     /gene="dhaK"
                     /locus_tag="IW774_000844"
     CDS             complement(14343..15326)
                     /gene="dhaK"
                     /locus_tag="IW774_000844"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1153209.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15328..15723)
                     /gene="dhaM"
                     /locus_tag="IW774_000845"
     CDS             complement(15328..15723)
                     /gene="dhaM"
                     /locus_tag="IW774_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1153210.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15877..16197
                     /locus_tag="IW774_000846"
     CDS             15877..16197
                     /locus_tag="IW774_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1153211.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16309..17178)
                     /locus_tag="IW774_000847"
     CDS             complement(16309..17178)
                     /locus_tag="IW774_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1153212.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17274..18578)
                     /locus_tag="IW774_000848"
     CDS             complement(17274..18578)
                     /locus_tag="IW774_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1153213.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18575..19084)
                     /locus_tag="IW774_000849"
     CDS             complement(18575..19084)
                     /locus_tag="IW774_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153214.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19259..21394)
                     /locus_tag="IW774_000850"
     CDS             complement(19259..21394)
                     /locus_tag="IW774_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1153215.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21477..23132)
                     /locus_tag="IW774_000851"
     CDS             complement(21477..23132)
                     /locus_tag="IW774_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1153216.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23134..23448)
                     /locus_tag="IW774_000852"
     CDS             complement(23134..23448)
                     /locus_tag="IW774_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1153217.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23618..23878
                     /locus_tag="IW774_000853"
     CDS             23618..23878
                     /locus_tag="IW774_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1153218.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23892..24206
                     /locus_tag="IW774_000854"
     CDS             23892..24206
                     /locus_tag="IW774_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1153219.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6930 a         5020 c         4390 g         7965 t
ORIGIN      
        1 cttccttcaa ttttttacac gtcgacgatt caataaaaaa ccgcctaata ttgtaaagac
       61 aggtgcaaga atcgagaaaa aggcgaatgg caagtaatgc aacgcgctaa ctttcatcgt
      121 tcccataatg aatgtgccgc tgactcccca cggtacgaga gaattgacgg tcgcacctga
      181 atccgctagt gttcgtgtca aataggtttt tgataagcca acgtcatcaa aagaacgctt
      241 gaatgtttct cctggaagaa tgatggacag atactgttct ccaacgatca gattgattcc
      301 gatacagctc aaggcagtaa acccgactaa acgagaaggt cggtccatct tttcttttaa
      361 ttcttgaacg atggtatcaa cgatcagata tttgatcagt aacccaccta atgccaatgc
      421 aagtaaaatc aaactagcag aacctaacat actcatgatc ccgcctcgag aaagcagttc
      481 atctagcttt ggatcaggtg tatttgcgac ataaccattc atcaagatgt ctgctgcttt
      541 tccgaccgtc aaacttttct catggataaa agaaagaacg agagcagctg tcgatcccgc
      601 tagtaatgta gggatagccg gaattttttt gattgccccc cccaaataaa ataactagtg
      661 gaatcaatgt ccaaggagag atccaaaagc tcgctttcat gttttggatc atttcattga
      721 tactagctag atcggctgaa tgactgttat gtcctaatat cgtataaaaa atcaacgaaa
      781 ggacgaatgc tgggaaatcc gtataacgca taccagcgat atgttcgaat agattaactc
      841 ctccaatacc cgctgccaag ttggtcgtat ctgataaagg cgagcagtta ttccctaaaa
      901 aggcaccgga aacgactgcg cctgtagtca ttgcatcatt gaatccaagt aaatgaccga
      961 tccccaaaaa agcaattcca atcgtcgaaa tcgttgtaaa tgaactgccg acagtcactc
     1021 cgacaattgc acaaacaaga aatgctgtcg gtaaaaagaa acgcacagaa acaagtttaa
     1081 atccataaac cataatcgta gggatggttc cagataaaat ccatgaagca actaagacac
     1141 caatcaaaag aaaaatcagg attggaatga tccctggagt gattcctttg atgatcccgt
     1201 catggatctc atcccatgtg aactttttaa atctgccata aaacaacaaa cacatgaaag
     1261 ctgcaaggat cgggatatgt ggcgagagct ggaatccgat gatcagtact cctaggagtg
     1321 ccaacaacac gatcaataca gcgatacttt cttttaaaga aatattttta ttcatcgtcg
     1381 atctcctatt tcaaatgaaa aggccctgcc atgaaaaaac tattcacttg gcaggacctc
     1441 ctactaaatc agcgacaaaa cgtcggctga aagacttatt tttttatttt tcttctaaac
     1501 ttttttctac ctcaatcccc caagaatctt tcttgaagtg attgatgatc aatggaataa
     1561 cgaacatgac cactgtgata gctgctacta ataatgcatc actcaatgtc gctgtaccta
     1621 atgtagcaaa tacgacaacg atgatagaga agatcagcat gcagctgact acccaagcta
     1681 atccgtttcc gtttttacca attcggtaag gacgttcgaa ttctggatgt ttcttacgca
     1741 ttttaagcaa tgaaacagca atcaaaatgt acacgataca ataaataaca gttgtagcat
     1801 ttgtaagtgt caagaataca gcacttacgt catccattac accataaagc aaagcaaata
     1861 atgaaataac gattgattgt gttagaacga catttctaga tacgccgtat ttgttttctt
     1921 tatggaatcc gaattttggt ggaaggaatc cttcacgagc tacttggatg attgtttttg
     1981 atggtccagt aacccaagct gataattgta aaagcacacc aataaatacc atgaagctga
     2041 agatatttcc gatcacttct ggtaagccta ggatttgaca gtcaatcaag ataggctgcg
     2101 tgatatttgc taattccatt tttccatctg gtacgacatt tgctacaagc atcgcattga
     2161 tgacattcaa taaaactaat ccgatcaaag cgataaatac gccttttgta taattttttg
     2221 ttgcgtcttt caaacgaggc atgtaaacag aactcatttc gatcccaaca aaaataaatg
     2281 tgataccagc taagtatttc aatgaatcaa tatgttccaa gtcagggaaa gcttttgacc
     2341 atgaaaagtc acctagatag ccgtttgaga ctagtccgac tttgaaggct gccaaaacac
     2401 ctaaaacgaa catgatgact actgggatat atacccctag ccatacaccg atgttcccgc
     2461 cgacttttgc catatcgaat ttcaagttca agatcgtgat gatccagtag ataactaaaa
     2521 tacaacctaa aacgaaccag tgattattgc caagttctac attaccaaat gtgtttccta
     2581 acaatggtcc taaagtagaa gcgaccatta ccatacctgg gaacatttgc acccataata
     2641 gccatgcaac gacaaatccc catttgctgc ccagtgctgt ttttacccat aactgaggac
     2701 cgccttcttg aggaagcatc gtggaaagct caccggaaac cattgatatc ggaccggcaa
     2761 aaaacaaaac tgcaaaaatt gaataaaaaa tcaatgtcca accgactgcg gctaacgtag
     2821 gaatactccg aaccgttgca cacagtgcca ttgtcagacc gataaaggtc ccaagaccca
     2881 ttttacttac ttcagattta ctcaccgttg acactctcct tgattttttg gataaaataa
     2941 cggtgtcttt gaagtagttt cagcaataaa cgaaacaatt cagcgaatat ttgaggggat
     3001 tctttgagtt tattgcttga agggtttctt caaagacaac ctcaaaacaa agggatcgaa
     3061 gagcttaggt ctccccttgc tcttcagatc caaacatgcg tcagaaacag ttagtattca
     3121 agctgttttc taacatgatt accttgctta ttttgcttcg cttgccaata attggtctgc
     3181 gtagattttt tccaattttt cttgaagagc tgctttgatt ttttcagcat attcttcaaa
     3241 gtgttcttct ttgttcatgt atggtgtcat tacgctggca cgcaatacag ttacttttcc
     3301 ggcacggttc cattcttcat ctgagaatcc tagttggttc acaaattgta atgggctgtt
     3361 gccatagtct gggatagcaa agtcagtatg tgaagttaag aactcattac cgtaaatgct
     3421 tcctttcacg tatgaagaat aatcatatac gtcatggttc aatttattca ttgcaactaa
     3481 gtcatcgttg cctttttctt tgaatacata gtctaccata ttgaaatctg gataagttaa
     3541 tggatgaact tcgatttctt tgtctcctac tttaaatgac aagtcattca agaagttgta
     3601 gaaacgatgt gcaccttcga ttgatgcacc cattaattta ccatatcctg tgacattcaa
     3661 aggtaagaca tggtgtgctg cccaaacgct ggctgctgtt gcccctgctt ttgaaccttc
     3721 caagatataa gccccaagta atgctgggat atctgcgcct ttttcaaata catatgtcgc
     3781 aaagtaagag ataacgtctc tcatgcggat atctttgatg acgataccac cggcagaata
     3841 tggaacatag cccattttat gtgggtcaat cgttacagat tctgcttctt cgattgcttt
     3901 atatgcactg tgtacttctt ctaagatata atctttgttt tctgtaaaga cattgtattt
     3961 gtaatgaaca tcttttaaat cttcaaatgg aatgaagttg ttgtcttcgt ctaagaaaat
     4021 cgcacggccg tatccgccat aagcagcatc tacgtgtaag tagaagtaaa taccgtcttt
     4081 ttctaatacg cgacgcaatg caacgatttt atcgataccg tcgattgctc cttcttctgt
     4141 tgatcctaca acaccgacta cacctaggat tggtgttttt tcagctgcta gaccacgaac
     4201 gattttttca agttcgttga tgtccatgcg atagttgtgg tcaactggca ctggaatcac
     4261 ttggtctaaa ccgataccga tgatgtcggc agcttttagc catgaatagt gtttagtttg
     4321 tggaaccaac cattttccta atttttctaa atgttttccg ctacgtgctg agtgagcttt
     4381 gatttcgtcg attttttctg ggacgctgtc taacagattc atgatttctt ctgttgacaa
     4441 gttcattaat tcccaatcgc tttttccagc aactagttca ggtgtcactt ctttcattgc
     4501 aagcggcaat gatttgatgt tgcgggcata ccaaagtcct tctaagtttg ctaaagaacc
     4561 atcagcaacg atgtggcccc agccatcttt atagctcatt aattttgcaa attccattcc
     4621 tacttcttct tccatttggc ttgttgcagg agaagattca taggcaacgt tattaccatt
     4681 ccatagcata gcaaaattgt acgctagtaa agatggcatc aatgtttcag agttcatatg
     4741 accccagtaa cgtccggcat tatgccaagg aacggaatgg gtacgcatcc gcgctgaaat
     4801 ttctgataga acatcctttg ttttattcac agtgtgttca aaactagcac tgcttttttc
     4861 ttctggcgta ataataggca tgtcttgagg catgtagttt tgacgccagc ctaaatgttc
     4921 atccaccaat tcattcaaca aagctttata gatttggccg ttctcagctt tgtccccaat
     4981 gaatagggca tttaggttta gatctttcga caatgattca ctcatagtga aaccctccta
     5041 aaaaaatatt taaattttgt cgagccctac attacacctg ttcttctatt ctgtaaacta
     5101 agttttggcc tgatttttaa tcatatttta atatgtatca attatccgaa ttgttcacac
     5161 ttttgaaata attaaaaaaa acgagggaat ttacaaatgt aaactccctc gcaaagattg
     5221 tattaacgac aaccagcgtt ctattttagg atgggtttca aaattggaat gttgtaatag
     5281 tatttccctt tttcaaggta aaaacttact aaattctaat caattacttt ggccaaaaag
     5341 taatttttct taccttttcg aataaccaca aacttaccat cgaattcatc ggatggattt
     5401 atgacaaaat ttaaatcggt aacgcggtca ccgttaatac ttatagctcc gtttgaaaca
     5461 tcttcacgtg cttgacgttt agatggttca atttttgtgg aaacaagcag ttccacgata
     5521 ttttcaggtg tgcttgaaat ttcaacgttc ggcattttac cgaatccttg cgcgatttct
     5581 tccgcattta gttctttaat atttccagag aacaatgctt gcgttatttt ttgcgcttct
     5641 ttcaagtctt cttcactatg cacaaatctt gtaacttctt ctgccaaacg gcgttgtgct
     5701 tcacgtttct caggttctgt ctctactttt ttcgctaagt cttcgatttc ttcttgagac
     5761 aagaaagtaa agaatttcaa gtatttcact acatcgcgat catcttgatt caaccagaat
     5821 tggtagaact caaatggtga agttttcttc ggatcaagcc aaacggcacc gcctgcagtt
     5881 tttccgaatt tcgttccgtc agccttcagc atcaatggga tcgttaaacc gaacgcttct
     5941 gcttctggtc cttctaattt tcggattagg tctaatccag ctgtgatatt tccccattgg
     6001 tctgctccac cgatttgcaa ttgcacatcg tatgttttat gcagatgcaa aaagtcgata
     6061 gattgcagga tttggtaagt aaactctgta aatgagattc ctacttccaa acggctggca
     6121 acgatatctt ttgctaacat cgtgttgatg ttgaagtttt taccgtagtc tctcaaaaat
     6181 tcaagtaaag agatttttga caaccagtcg tagttgttca caaatgtgat atttgcatct
     6241 ttaccaaaca attttctcat ttgattggaa agtgcgtcca cgttatgttg cacttgttcc
     6301 atcgtttgca acacacgttc tgttttgcgg ccgcttgggt cgccgatcgt tcctgttccg
     6361 ccgccaatca gaatatatgg atgatggcct gccaattgga atcttttcat catcataaat
     6421 ggaatcaaat gtccgatatg catcgaatca ccagttggat caacgccgca ataaagtgaa
     6481 atgctctttt cttcaacgag ctcgcgaagt ctttcttcgt ttgtttgctg attaatggcg
     6541 tcgcgccaag ttaattcatc aataatattc attttactga cctccgtgta gtttttttat
     6601 gaaagcagga acctgctagc atgtgtaatt aattatttct tcaggatagg ataggaaata
     6661 aaaaaagtcc cttggcatat aagccaaggg acgaaatctc gcggtaccac ccaatttgta
     6721 tgtctagata tccaacatac ctctcgaaca tttaacgctg tcgcgtggca atcactgcca
     6781 aagactcctg aaatgtaatt cgctttaaag catgtactgg tttgcagcga ccaccagctt
     6841 tctgaaacat tggctttaac aactacttcg tttcgatctt cgtcttgtta ttattaatat
     6901 gtgtctatca tagagcgcaa ctttttcttt gtcaacagaa aaaggatatt ttttttaaat
     6961 tcctcatttt ttcttttttg aatccgctct aattccgaac attttatttt caattattgt
     7021 taaaagaaat aactttttct gtattttttc tgcaatttat cgagaagtca ctttactttg
     7081 cctcttattc tttcttcgtt acaataagga atgggacaag taaaaaaatg aaaaagcaag
     7141 ggacgaggta tatggaaaaa aagcaaaaaa ttattttagg agccacttct gctttaacta
     7201 tcgttatttt aattctaagt ttgttttttg tcatccgatc atctacaaaa aaagaaccaa
     7261 tcattcctga aaaaaaaata acagaaaaag caaaagaacc gacagatgat aagcaacaac
     7321 tagttactca agcaaaaaat ctggctcaaa gctacgatta tgaccaagcg attgcgttat
     7381 tggaaaaaga tagtaagaac ggatctactg aaacacaaca gcttcttcaa acactaaaaa
     7441 aggagaaaga acaactgatc gactggtctg atcctactca gatctctcat gtctttttcc
     7501 acagcttgat cgtggacccg caaaaagcct tccactctca acaggcacaa ggatacaaag
     7561 attatatggt gactgttgaa gaattcaacc gttcgatcga acaactttat caaaatgatt
     7621 ttgttttagt taatttgaat gatttgatcc aaaaagacga acaaggaaat ctttccttta
     7681 acgggcttgc gctacctgaa ggaaaaaaac cattggttct ttcacaagat gatgtcagct
     7741 attatgaata tatggataat tccggttttc ctagccgatt ggtcgtatca aataacaatg
     7801 aaatcaaaaa tgtctataaa gagaaagata aagaagaaat cggtgacttt gacatggttc
     7861 ctctgatcga tgcctttgtc aagaaacatc ctgatttttc ttatcgaggc gcaaaaggta
     7921 ccttggccct gacaggttac aacggagttt taggttatcg aacatcaaaa tctcaatatg
     7981 gagacaatga aaaaacaaaa aaagagatta aagaagctaa aaaagtggcg gaacagttga
     8041 agaaagacgg ctggagcttt gcttcccata catggggtca cttgaatatg actcaagctt
     8101 cactagaaga tgtaaaaaaa gacaatgaat tgtggcagca agaagttgca cctatcctag
     8161 gaaaaacaaa tattctgatt tatccatttg gtgcagacat cagcgactgg cagccttatt
     8221 cagcagaaaa tcaaaaattt gcttacttga agagtcaagg tttcgatatt ttctgtaatg
     8281 tcgatgcttc tacaccagct tggggacaat taggtacgga tttctatcga aatgctcgga
     8341 tcaatatcga tggcattcgt tttcaatctg atttagacgg aaaaaatcct attcttgatc
     8401 cattcatcaa tgtccatgat gtctatgaca aagaagcacg cgaatctgct tgattttacg
     8461 tcatttatga accaaaaaag ccgaactgac attttatcgt cagtttcggc ttttttatgc
     8521 tttatttctt cttcggtgcg attcccttga tcaggacttc tgtccattct gctgtatatg
     8581 gaagcagttt tgcgtatatc tccggcttag aaattccttc taccattgct tgaaaataga
     8641 ggatcaagaa ctcatccgaa tatttcaaat ccacttttcc ttctttccgc cctcgatgga
     8701 aaagatcaag catgatactg aaactttctt gcgtatacgc atccatcgcg tcagaaaatt
     8761 cttttttttc tttttttgta tagtatgtca tcaaatcgat ataaaattgt ttgttcactc
     8821 gattgagata acggattttg ttttcactca tggcaatcag tgtttcttca aatggaagat
     8881 tttccgcaat caacgctttc gcttcttgcc ctaaatcatt cataaatgca aaaaatactt
     8941 cataaatcag cccttccttg ctgccaaaat atttaaaaat cgtcgcttta cttacctgag
     9001 cgttctcagc taccttatcc attgtcactc cagcggttcc tatgtctgtg ttcatcaagt
     9061 caaaagctgc ttgtaaaatc tctttctttt tctcttgtgc tctcttttca aaaccgttca
     9121 tttgtagagc ctcccgtaat tttaaacttt tataatatat atagttcaaa tttattctga
     9181 ggtttattat aactatctat tgagaaagta tcaagctagc aattccttgt ttatgaattc
     9241 attgacagaa atttataaaa agaatattat gtactaaaat aataaaaaaa gttttataaa
     9301 aaggtataaa acctttttat taaactattt aatcttgttt agtatatatt tttagaaaaa
     9361 ggagtggggt ttttatggca gaaatcgtca aagtcacaca tttgaaaaag agctttggtc
     9421 gtttcgaagc tttaaaagat gtctctttca ccgtacattc aggtgaagtc gttggattta
     9481 tcggaccaaa tggttctgga aaatccacga cgattcggac acttttaggg attataaaaa
     9541 aagacggcgg aacggcaacg atttttgggc aagatgtatg ggaagatagt ttatctatcc
     9601 atgaacgtct ttcttatgtt ccaggcgata tctctttgtg gggaaatttg actggaggag
     9661 cgatcatcga tctcttcatg aagcttcatg gaaaagggag tgctcaaaaa aaagatgaac
     9721 tgattcaacg tttctctctt gatcctaaga aaaaagcaaa aaactattca aaaggaaatc
     9781 gccaaaaagt gggcttgatt gctgctttgg ctgttgattc ggatctctat ttatttgatg
     9841 agcctacttc tggtttagat cctttgatgg aaacgatttt ccaagaagaa gttgaaaaat
     9901 tgaaagaaca gggcaaatcc atcctacttt cttctcatat tctcagtgaa gtcgagcgac
     9961 tagcggataa agttgtgatc atacgcgaag gaacactcga tgaagaagga acactcgatg
    10021 aattgcgtca tttgactcgt tcgaccatca ctatagaaac agaagcagag aatagtcact
    10081 tagcacaatt gaacggtgtc catgactttg tgcaaaaagg tcacctcgct caatttgctg
    10141 ctgataacga agctttcgat gtgattttag ctgaagcgtc caaattaggt gtgaaaaaat
    10201 ttgaatctat tccaccgact cttgaggatt tattcatgag acattatgaa ggatgaggag
    10261 gaagcgaaat gactgataaa ttttcacgct ggtccaccct tttttctcaa aaccttagcc
    10321 gtgactggaa aaagatcctg atatggctag tcggtgtcac tgctttttct ggtggttttg
    10381 tccctgcgtt tgaagaattg acttccggac aaggattagc tggattatac gaaaccttgc
    10441 aaaacccggc aatgattgca atggtcggac caactccagc ttcgaatgct gctgagtata
    10501 ctttaggagc gatgtatgca aatgaaatgt tgcttttttg cagtttgttt gctgctgtga
    10561 tctctgtttt acacgtggtg agccaaacga gaaaaaaaga agaacttggt ctgattgaac
    10621 ttattcgttc ctttcgagtc ggacgtcaag caaactcttt agctgtcatc gctgaaagta
    10681 ttctgatcaa tttgctttta gcaatatcga tcagttgtac gatgattttt ttccaagcgg
    10741 attcgatcac tgcagaaggt gcttggcttt ttgggctatc tgtaggtagt atggggattt
    10801 tggggagtgc cgttgcactg tttgctgcac aactgatgcc aagttcagcc agttcgaatg
    10861 gtctgtcttt agcgttttta ggtggacttt accttctacg agcaataaca gatgtagctg
    10921 attcctccct ttcgatgtgg aatcctttag gctggtctta tttgacctat ccatttacaa
    10981 ctaacaactg gattcctttg ctttatttgt ttttgttcag tctaatcttg ataatcagtt
    11041 catttgtttt agaggaacat cgagatttga acaccggcta tatccccgaa cgtgaaggaa
    11101 aaccgtatgc taaacgttct cttttatcct tgcgtggttt attattctat ctaaataaag
    11161 ggatgacaat cagttggctt atcacttttg gtttgatggg ggcggcatac ggctctatct
    11221 acgggaatat gcaacagttt ttagaaagta acgacttaat gaaacagatg tttatccaat
    11281 ctggcgtctc aattgaaaaa aactttacaa gcacgatcat ggtcgtcttg atttgtctag
    11341 ctgctatttt accaattgct agcatcaatc gtttatttac agaagaaacc cacggacgta
    11401 tgaatcagct gttcgcaaca aaaatttcta ggaaccaact ttattggacc aatatgttac
    11461 tagctattct atttgctata ttggcaatcc tgattagttc tggtgcacta gggctttctg
    11521 ctttggcttc gatgaaggat gcgaaagaca tggatctatt gatgtttctt aaagccgggt
    11581 tcaatttcct gcctgccgtt ttagtgttta tcggaattag tggctttcta ttcggatggc
    11641 ttcctcgaat cggtaaactt gcttatatct acttagctta ctcctttgca ctcaattact
    11701 ttggcggaat tttggattta ccagaatggt tttccaaaac agctgtccct agctgggttc
    11761 cacggatgcc aaacgattct tttgatcttt ccgtgttcct tacgttatca tgtatcagcc
    11821 ttgcactgtt cgtccttggt tctattggtt ataggcgaag agacttacta gaaggataat
    11881 aaaaaagcat gtacaatttc atgctagcct ccaaatctgg gtcaaaaatc caacatttgg
    11941 aggtcatttt tttatttaca agtcttctaa ctgctcttta tcaatgatcc agacttctcg
    12001 attctttctt tttacataac cattctcctc taataattta aattttctag ataaagtttc
    12061 gggggtcgta ccgataaatg cagcaagttc tttcattttc attggcagga aaaagtgatc
    12121 cttccctgtc gctttagcta agtcaagcag atagacagcg actcgttctt ctactttttc
    12181 catcatcaaa aaagttgtct gctgctgtac ttttgttaat ttctcaacgt tgattgctaa
    12241 aagtttcaga ctaagggcgg gataatgcag taagagttcc gagaaatctt tttgtctcaa
    12301 tatgcaaatt tccgtttttt ctagtgcttc gccaaataaa gactcatttg gaacgcctag
    12361 caactggctc tctccctcat atccgcccgg ctctattact cgtaaaagct gttcctttcc
    12421 gctagcagat aattgataca ctttcatact gccggaggcc acgatcacta attgaggttc
    12481 tccattcggc gcaagaatct gttctccttt ttccacatag tgatgtttga caagtgcgtg
    12541 tactttttct tgatcttgct gtactaagtg attgaacaaa ggaacaaatg aaacacataa
    12601 atgatcatga ctcataatct ctcctccttc taaacagcta tttctaataa agaaacataa
    12661 cttcccttat tccaactgat aattttcttt tatcatagca taaacgtcaa gcgaaccaga
    12721 taccagcaaa tagagtggat ttctttttgt cccttgcttt attttccttc ttttgagacc
    12781 ttaagtaatt gattcctatc aattttttaa atatttactt tttgaataat caaaaacaaa
    12841 ccttcactcg caagccgaga gtaaggtttg tttttcgcat tgcttatttc gtgttgtcta
    12901 ttgaaatttt tccgcaccta gcttttgata tagtggtgct agttcttgta aggtttcagc
    12961 ggttatttgc tcaacgtgtt cgatttcttg cggatagaag aatagtttac ccatttgaaa
    13021 ggttcggtct ctcatcgctt ttttcaactc ttcttgtttc tttgtatcgg acaaatagtc
    13081 agacaaagct aagtggtcgt caagttcatg ttctggttgc ggccatatgt aataatcctt
    13141 gatatcgact gttttgctcc actctttcag tatcgcaagc aactggttat acgctgcaat
    13201 gctaccatat cgttcttcgc ttttataatg ttggtacatc aaatagattt ggtattgttt
    13261 ttgattcaac aaaactgcta gacaggcatt ctcgtcttga cgagattcgc ttcggtacgc
    13321 gctccaaaaa tgactgcgta aattccagcc attcgtccag ctttcgattt taggtttgct
    13381 catgccgtac atatcaggca aaagtgcagc tgtctggagc tgaatctctt tccatttttg
    13441 ccagtgttct ttatattctt tcttgatttg ttcaatctta tctgttggat ggctctcttt
    13501 gagctgtttg aatgcgtaga ttggtcgatc aaacaactga taatctgatt ttccaagttc
    13561 cattgtttcg cctctttccg ttatcttttg tattctacct gaattttgcg atatggacaa
    13621 atagaggttg tgaaaaagct gttctgcatc aagtcataag aacagccaaa caaaaaactg
    13681 cgatgagcat caccgcagtt ttattgaact taaggttgat tgatcacatc taaaagcgtt
    13741 gaaaataaaa tggcactaga agcagctcct gggtcgatat gtccgattgc acgctctcct
    13801 agataggaag ctcgtccttt tttagctacc aagactttgg tgtgttcttt tgcctggtca
    13861 atgatttctt ttgttaaatt ccctgccttc aatggctctg tgactggcag ccaaacatct
    13921 aacatcgttt tatcgtcagc ttctgccttt cctcggcttt gaatacccgc tgttccctgc
    13981 tcgatcactt cgcctaattt ttcttgagaa tcaatagaat cgacctcttt tgtcgctttc
    14041 atcatgttca tgaatgcaga tccgtataac ggaccagaag ccccaccgac tttcgatatc
    14101 atcgccatag agaatacttt gaatgtatcg ctgattgttt caggatgatc ggcttgaaaa
    14161 gcttcttcat aagctgaaac ccctcttccc atgttatttc catggtctcc atcgccaatc
    14221 ggggtatcta gatcactgag atattgtttg ttctgattga ttgcctctgc aaattgcgtg
    14281 agccattctt ggatttcttg aacggttagt ttcatattat caccctttta tcttgagatt
    14341 ttttaccatg aaatcgtctg tacattgctt tctaaagaat ctttccattg tgtctccgtc
    14401 aagtcgatca gtgttagaga aagaccttgc atatctagtg aggtcataaa attaccgact
    14461 tttcggaaaa ctacattgac gcctctttct tccaacagag ctaaaacatc attcatgaac
    14521 acgaactgtt ccatcaaagg cgtcccgccc attccgttga ctagtacacc tgcttctttt
    14581 ggtaaattcg agtaatcatc taaaatctta gtaacaagtt cctttgccag tactttagaa
    14641 ggctgcatct tttctcgacg atagcctggc tcaccgtgga taccaacacc atactcgatc
    14701 tcatcttctc ctagttcgaa tcctggtttc cctacttctg gtacagttgc tgctcgaagc
    14761 gctagaccga tcgtcttagt tgcctgtacg accttttctc ccagttcttt caactcagct
    14821 agggaagcac cgtttcttgc tgcatcgccc acgattttgt gtacaaggac agtacctgca
    14881 actccgcgtt ttccagcagt atacgtacta ttttcaacgg cgatatcatc atctacgatc
    14941 acactttcta cttcaatatc gtccatagca gctaattcct ttgccatatc gaagttcaat
    15001 gcatcccctg tataattctt aacgatcagc aaaactcctt ttccactatc ggcttcctta
    15061 atagccgtct gaatctgatc tggagttgga gaagtaaaca catcaccaag caccgctgca
    15121 cttaacatcc cttcccctac aaacccggca tgggatggtt catgtccgct tcctccacca
    15181 gaaaccaaac caacttgttc cgctttctga ttcttggcta caacacggga agattctaca
    15241 cgatgaacaa gttctggata gctttttact aatccttcca gcatttcttc gacaatattg
    15301 cttggatcat tcatgatttt tttcaattta ttcccatcct ttctttccgg cattttgtgc
    15361 ttctgttaag atttgctgag tatcatctgt tataccggca gtgatggctg ctgcaaaagc
    15421 tccttccact aaaggcgcat ccaccagatg ataacgttgc tgctgctctt cttccaacat
    15481 gtcttttgcc agttccgagt tcaataccgc gctaccgata tccgcaaaaa caaaataact
    15541 agctgcatcg cttgattcat tgactgcttc tacgattttc atcgggtctg atccgatctc
    15601 gccatttatt ccgccgagag aaaaaatttg cacctcttcg gatttttgca tctgctcaat
    15661 catttcttta ataccatctg ttactttttg actatgagaa ataagcaaaa tacttttttt
    15721 cacaagaatc ttcctttctt ttacatagtt gctaacgatt ggtttcgctt tcattaatga
    15781 acaattcaag tatataataa atttctaatt ttgggaaaat atgtgtagtg cttcttgaag
    15841 tttgttatac tttaattaaa agagaggagt gatcgaatga ttacaacaac aacgaacagt
    15901 gtagagggaa aagccgtagt agcttataaa gggatcgttt ttggtgaggt cattactggg
    15961 atccatgcat ttaaagactt ggaggcgggg ttgcgaaata tctttggcgg ccgctcaaaa
    16021 agctatgaaa acgaactaat gggtgcaaga gaagaagcac ttgccgaaat ggccgaacgt
    16081 gcaaaagcat taggagctga tgcgatcatt ggcgtaaaaa tggactatga agtattaggc
    16141 agcgacaatg gcatgctgat ggtcacatgt agtggaacag ctgtctccac gagataagta
    16201 ggggacatct gttttaataa ctaaagagag atgggacatg tagtggcact caagaattag
    16261 atttttcagt ctaacttcga ggtactctac cttcccatcc tctctttttt atttcttttt
    16321 gcggatgatt ccgtaagctg ctgcgactaa cacacccgcc cctgctaagg aataactgac
    16381 agaagctgtt ttttcatttg tttttggcaa agtctgactt gttgatttgg atggctctgt
    16441 cgtttgctga actgtttctg tcgtgcttcc agaggtcact gatttttctc ctgccgaatg
    16501 caaaatatct tttattcgtt ggaaactagc tgctaagtca gctttttttg cttcaaaatc
    16561 attagcaact gtttgtgctt cgctgatctt tccgtggatt gcttgactgg ccgctgtgat
    16621 atccgcttga gtttgactta gctttccaga agctgtttga gcaacctgat ttaattgctg
    16681 attgaattct tttgcttgtt ggatcttccc gtcaagatct gtttggacag cttccgcttt
    16741 tgctttcgct tcggcaagct ttttctcaag ttgtgcacgg tcatttccca ctttattttt
    16801 aagatcagct aaatcttgat cgattgctgc ttggtcttct ttcaatttgt tttttgcagc
    16861 ttctaatgct tcttcgagtt cttttctttt attttccaag tcttgcaaat gatcgatttg
    16921 gtcagaagag gtagaactat ttattgatgt atcttctgaa gatgaaatcg tttctcctga
    16981 attatctgtc ggtacattgg aagtcatttc tgtttccgag gcaccgtttt cagcaccaga
    17041 agagcttatg gttgttgaag gttctgtttg agcaggttca gaagtttccg caaaagctgt
    17101 ctgtcccaat aagaaaatac tggaaacagc aattggccag attaacggct tcattctttt
    17161 tttcatcact tttcccatcc ttactacttt ttaactaatt tcttattata agcaaaaatg
    17221 tttggaagaa ccataaacac aacaggctgt ttgtctaaaa aacggtgcat cttttatttt
    17281 tttgtacgtt ccgggctttt ccatgtcccg cttccataac ctaaaatcgt tttgactgct
    17341 ggaatgaaag tcgtcacgat cgttatcgcg ttcactatcc agaacaaaag catatatagt
    17401 ggtgcgaaaa gaaggtattt caattttctt cgtcgatcat ctacaatcag cgaagctagc
    17461 aactggaaaa atcctgcgac catttcaaag cagatgaaca caaaactgaa tgtaaaagta
    17521 atataaatct gttcgtagtt tccttgatat acataatgcc cgattaatcc agcaaaaaga
    17581 actacggaaa tacaaaagaa tattgaccag acaatactca atgtttgatc aatgaagatg
    17641 atcgttcttc caatatgttt aaaaggatgg agcatcactt ttttgaaatt cgtcagccag
    17701 acttctgttc caccttttgc ccaacgtttt ctgtgtcgat atagcatctt caacgtcaca
    17761 ggtacttcca tgaaaaagat aatctcagga gcaaaaacag ataaccagtc atttaactga
    17821 tgatcccaag caatactgat gtcttctgtc gctcgatctt gtctgaatcc gcccacatcg
    17881 atcaaggctt cttttcgata catcgtattt gctccactgt acgcataaag accaccgaaa
    17941 acagctgact gtgttcgttt aatgatgccc acgatacttg agaattcaac cgtttgagat
    18001 tttgcaatca atttggtacg attttggaca tccatatttg cagtgaccgc agcaatatgt
    18061 cttgcaccag ggcgaatgaa ataattcaca tacctaatca gtgcatctgg ctccggtacc
    18121 gtgtctgcat cattgcttaa aatcaatttc ccttttgcaa aggcaagtcc gatattaaac
    18181 gcgtgggctt ttcctttatt tttttcgatc cgaaccacac gaagattagc gtattttttc
    18241 atcagtctag ctaatatttc tggtgtttgg tccgttgaac catcgtctgt cactaatacc
    18301 tcatagttgt gataattcaa tttcgtcatc aaatactcga ttgtatattc gatcacaatt
    18361 tcttcattat gggctggcac catgatcgtg atgaacggtt cgacactcaa gggaacatct
    18421 acccattctt tttgcttatg tttgtataaa aacacataac accagacacc aataaaccat
    18481 acaaatccgc ctaaaatcgg ataagaaacg agaaaaaaat acataataga caaacaagta
    18541 tctaagaata aattacctgt atgggtgatt gagatcattt cagttccacc ccattttctt
    18601 tatacaaatc tgcaatgaat gtcgtatcta gatttttctc ttcggagaca gaataaaaac
    18661 ggaccgtttc tctctcttct tttgttccaa aacgttccgt atacacttct tcaagtaatt
    18721 cttgtctttt tttcaatttt tcatcttcat attcaaaaga tttatttact tttttgtata
    18781 gatttcgatt gttccaccta gtcaatacaa atgaggtaat caggaaactg aaaaaaatca
    18841 atacaaaaaa gccgattaag aaatacagca ttttcttttc tgataaaaaa gcgtacaagg
    18901 aaaaatcctt ggctctttct ggtctcgtta atgggaaaaa gacccatata aaaggaagaa
    18961 caacgatcat ccatcctaaa agagccacta acgtttgcca aatctttaac cagtaattac
    19021 cttttgaaaa atacttatct ttcaccaaaa ttgatgcaac ttcttctctt gttacttttt
    19081 tcatatcctc acctcacgtt tttttgttac gacgtaggag tttaccatac atttcatttc
    19141 ttttcacata aagtgttcct aaatttttcc taagaaaaag aacaaaacat ttctcatcag
    19201 caaatgaaaa atattttgtt ctttttatca gttgttcttt ttatttttta tctcttaatt
    19261 atgttgctct ttccaatacg acaatgcttt tcattcctct agatatcgcc gtgtacattt
    19321 gtctgcgtct tctttgctca tctccatcgt tttcaaatgg atataagatt acatgatcaa
    19381 attccagtcc ttttgctaga ctcaaaggaa tgatttggca gttttctaat ccatatgaat
    19441 tttttttaat cgattcttgt agcgtttggg tttcctcttc tgtttgggtg ataactgccg
    19501 ctttttctcc ttttagagaa accaaaatct cagctagtat tttcaacaat tcttcagagg
    19561 atcgaaaaga gcggtattcg ggttcttttc cttgcggtcg gatcgatacg atgtcgatct
    19621 tctcttgatc cacagcatag gttttgaaaa gctctgtgat tgctccgcta gaacggtaac
    19681 tatttctaag atcataccga tggatcggca gatggtagtc gtcgaaacaa cgcataatat
    19741 cagcaaaagt agtggaagaa ttaaagattg cctgattctc gtcacctact aaagtaaagt
    19801 gagtttttgg aaataattcg atcaatagac ctaattgtgc cagggtataa tcttgtactt
    19861 catcaatcaa tagatagcga aatgctggta catttacctt ttcaacaagt aaatgacgaa
    19921 tagctaatat aatgactgct tcatcaacag tacgtgtggg ttgccaagca taagctgagc
    19981 catatcgttt ttcataaatt ctttcaaaca actgatgctc atccacccat gccatttctt
    20041 cgatttgtct ggttattttt cgatattttt tttgtaaaag tttccttgca taagctacaa
    20101 tactttgttc cgaatcatca gaaatcaatt taccgaataa ttccaactgc ctatcttctg
    20161 tcaacgagag tacctgattc tgcacagcag gactttttgc ttgtttcaaa agatgattct
    20221 tccattcgct catcagtgcc tttttcgttg cttgaagcct atcgatcaat ttaggatgag
    20281 caggaacttt ctcatatatt ttttcgatcg tttttttaga aaggactgtt tttccatttt
    20341 tagtaagtcc acggataaaa ttcggatgat ctaaaaacaa aggatctgat tgttttagat
    20401 tatcgataaa ctttttgcta cgcaaacgtt ctgtctgctc cgatacattt tctcccgtga
    20461 tacgtttgaa ataagcctct tcaccttcta tttcctctgc tgaaagctgt gaaaccagct
    20521 gtatgatggt tagattcagt ggatttcttt cacctaatga tggtaggaca ttcgaaatat
    20581 agtcgataaa ccgatgatta ggtgatagaa tcagcaagtc atcggatgtc atcgtatctc
    20641 gatataaata gagcagatac gcaatacgct gcatgatagc agaggtttta ccacttcctg
    20701 ctacgccatt taccaagata ttttgacttt tcacatcccg aatgattgtg ttttgttccg
    20761 attggatcgt agcagtgata gccttcattt cattggtctc atcttggcct aagacttcta
    20821 ataatacgtc atcttgaata gctacagacg tatcaaaaaa cttgaccaaa cgatctcttt
    20881 ccaaaagaaa ttgtcgtctt tctttaataa ccgtatcgat tttattttga cgaaccgtat
    20941 aagaagatgg accaatcatg ttattgtaaa aaagttcggc aattggtgaa cgccaatcat
    21001 aaacaagatt ttcatctgca tcatctgtga aatttgctgt accgatataa aaggattctt
    21061 ttggctcatt atccagaaag tctacaacga cttttccaaa gtagggactt ttcaataaac
    21121 gttcgatctt tgctagctga ttttctgcgt ttttctgttt gatattcatc tgatcgattt
    21181 ctctattttt catttcaatc atcgaaaaag tatccaggtt atctaagtaa ctgtcaaaat
    21241 tcaatcgaag ctcgccagcc atttctttca acgactggtt tccttctatt tttgccgtat
    21301 ccatcgattc ccgcagttcg ttttttttat caattagctt ttgatacacg ttcttcagat
    21361 atgcttcttc taactgtttt tgtgtgttga ccatacacat tctcctcttt ttttaaagtg
    21421 gtggaaccga ttagtttacc tcttttaaga gtcgaatgca agctttttaa cgagtatcat
    21481 ttgttaggat atatcagttc ttcaggtcgg acagtttgat ataaagaaaa tgcaccatca
    21541 aggtttttta cgtggtagcc agactgcttc aagattcttt ccgctagata gctacgtaat
    21601 ccgctatgac agctgacgat atattcttgt tggctgtcta attcatttaa tcggtcccga
    21661 agttcattca aagggatatg tttggcttcc ttgaatttgc ccttttccaa ttcttctgct
    21721 gttcgcacat ctaataaaat ttttcctttt gctagttctg tcggcaattc atgccattgt
    21781 acggtttcac taagcccttc tgcaatgttc attgctgcat atcctagcat gtttactgga
    21841 tctttggctg agccaaatgg cggtgcatac gtcaattcta attccggtaa atcgaagatc
    21901 gtcaaatgcc ctttgattgc tgttgctaaa atatcgatcc gtttatccac accttttgcc
    21961 ccaacacctt gtgcaccata aatctcgcca gtctctggat ggaagatcaa cttcagtaaa
    22021 atatctgttg cttctggata atagcttgca tgatcttttc ctgatacatg aatgacttca
    22081 aaagacagtt cgttttcttt ggctgttctt tcacttaaac cggtggatgc ggcagtcatt
    22141 ccaaacgttc gtataattgc tgttccaata ccgccttgat ttcttcgaga aattccggaa
    22201 atcgtgtccg caacttgtct accttgacga ttggctggtg aagcaagaga aataagtgca
    22261 tcttttccag tgatttgctg ttttacaacg atagcatccc ccaccgcata aatatcagga
    22321 atattcgttt gataacgttc gtctactagg atacccccgc gtatgcctaa ttcgattcca
    22381 gcggtcactg ctagctggtt atcaggacga acaccaatgg ataaaataac taaatcagca
    22441 gggatggtac gcccatcatc cagtcgcaat cgtttcccgt ggtcttcaaa tccagcgaca
    22501 gcatgagaag taatcacttc tactccttga tgagacaatt ctttttctat aaaagctgcc
    22561 atttcttcat ccagtgttgg aagaatatgt ggtgcttgtt cgatcaccat gacttctaaa
    22621 cctcttcttt ttaggttttc tgccatttcc aatccgatga atcctgcacc aacgatcacg
    22681 gcacgctttg gctgtttttc taatgcatgc atcactttat cgatatctgg tacatttcta
    22741 atagaaaaaa cattgtcggc ttctgttatc cctggaatcg atggaacaac tggttttgca
    22801 ccaggagata aaaccaatgc atcgtactgt tcaatgtgtt tttgaccctc atgtacgact
    22861 tctaccgttt tgttttcggg aaagatggct gtcacttcat gatttggacg cacatctaaa
    22921 caaaaacgag cggataatga ttctggcgtt tggacaagaa gattttcccg ttcactgatt
    22981 tctcccgaaa gataataagg caagccgcaa tttgcaaaag aaacatacgg tcctttttca
    23041 aaaacaacga tttctgcatc ttccattaat cggcgaagcc gtgtagcagc agacattcca
    23101 cctgctacac ctccgacaat aacgattttc atcttatttc cctcctactg ttcgatcaaa
    23161 ccattgattc atgccaccac gtacattcac tacatcataa ccagatttct ttaattcttt
    23221 agctgcttgt ttactcctca tccctgattg acaaatcaca tagacggtct tttctttgtc
    23281 tccatcatat ctgtttatac tttgtaatgg aacattttta gctcctttta tatgcccgcc
    23341 ccggtattct gcaggtgttc taacatcaag taacgctgga ttttccaaaa gcttttcctt
    23401 taattctggg actgttatcg attttattga ttgaaatagc aaattcattt tctgcacctc
    23461 atttacattt ttataaatac ctcatggggt atattaaaca ggagctttat taatatgtca
    23521 accagaatga ctcttcattt ttttcactac tttttatatt tgcataaaat accgataagg
    23581 ggtattatct aattataaaa atagaggaga atgtgttatg gagattgata ataaaaatat
    23641 cctgaaccgt ttgaaacgta cagagggaca aattcgtggt atccaaaaaa tgatcgaaga
    23701 cgaaaaagaa tgtatggatg tcatcactca attaagcgct gttcgttcta gtattgatcg
    23761 cgtaatgggc atgatcgttg cagataatct gaaaaattgc tttgagaaac ctgatagtaa
    23821 tccagaagaa caagcggcta aactagaaca agcgatcaag atgattatta aaaagtaaag
    23881 gaatgatttc aatgaaagtc gttttccata ttgatgaatt agaaaaatgg tcagaaacag
    23941 ggaaaaacgt aaaaaacctg ataaaagctt ctccagaaac gacgattgtc gtatctgtaa
    24001 atggcatcgc gattaccggt tatctagact ctgccaatgc agaattctta gatttgcaag
    24061 gagtcgtttt tcacgcttgc gccaatgcta tgcgtgctaa tcatatttca gaaagttctt
    24121 tacctgaaca agtaatcgtt gtaccagcag gtgtccttga tttagttgaa ctgcaatccc
    24181 aaggttatgc ttatatcaag ccttagtcag tagaggttgc gacagaagtg atcagcttca
    24241 agaaatttcg gcttattttt cgaagaagct acttctggaa aaccgtgaat caaaggttgt
    24301 gaaaa
//
LOCUS       DACEOE010000025        23509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEOE010000025 DACEOE010000000
VERSION     DACEOE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.026"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..600
                     /locus_tag="IW774_000855"
     CDS             <1..600
                     /locus_tag="IW774_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153220.1"
                     /translation="ADAEFTLTDEKGVKEVKKTDATGLATFNIIANKTYNLEETKVPT
                     GYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQGKALANAEFTLTDDKGV
                     KVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEGITVADDGQIFEYTVINH
                     RVPETVRTGGIGTSAFLLVFLVAGLGMGLLYYREQRKVK"
     gene            649..2484
                     /locus_tag="IW774_000856"
     CDS             649..2484
                     /locus_tag="IW774_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1153221.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSFGLGEQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGKPVSDIDNKGKEIALPNGVQPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPVQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     DYEKYVVKDVLGEELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTDSGMDKLQKKFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNRVTYLKQDPTTKAITWVEDKSKATEFVSGTDGKFQ
                     VEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSK
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            2553..3416
                     /locus_tag="IW774_000857"
     CDS             2553..3416
                     /locus_tag="IW774_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1153222.1"
                     /translation="MSMKKKNIKLIILRIGMLLCLGVGLYPVITQAYTYYQTQQTLNA
                     YKEKYDEASEKENEEIFKKYSEINRKRKETEQTLVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYNGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFINVAGKKLAYKVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     ENSHRLLVRGVRIPYKKNIVEKEKKKEDLKVKAQINWYPIIVVGVLSIIALIVIIKKI
                     VAKRRGMKNEE"
     gene            3406..4485
                     /locus_tag="IW774_000858"
     CDS             3406..4485
                     /locus_tag="IW774_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1153223.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIDK
                     KQTPSKEEVAKNVGQILSNNENQKVNETQESDNESFDMDEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYIYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRSDVIKNYGQNSTLTDIPDL
                     DMLTLYTCHPFPQNYDRLLVRSLRDDKAKYITPQVTVGNTAYADAIDSESVSAKPETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQTSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVLGALASLKVLLLLIKEFRKA"
     gene            4824..5624
                     /locus_tag="IW774_000859"
     CDS             4824..5624
                     /locus_tag="IW774_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153224.1"
                     /translation="MVVISKTALDSLAKVFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            5712..6518
                     /locus_tag="IW774_000860"
     CDS             5712..6518
                     /locus_tag="IW774_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153225.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            6750..7619
                     /locus_tag="IW774_000861"
     CDS             6750..7619
                     /locus_tag="IW774_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1153226.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVKAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            7639..7986
                     /locus_tag="IW774_000862"
     CDS             7639..7986
                     /locus_tag="IW774_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153227.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            7999..9261
                     /locus_tag="IW774_000863"
     CDS             7999..9261
                     /locus_tag="IW774_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153228.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDTREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            9341..10717
                     /locus_tag="IW774_000864"
     CDS             9341..10717
                     /locus_tag="IW774_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ1153229.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGDGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            10714..11343
                     /locus_tag="IW774_000865"
     CDS             10714..11343
                     /locus_tag="IW774_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153230.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            11362..11841
                     /locus_tag="IW774_000866"
     CDS             11362..11841
                     /locus_tag="IW774_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153231.1"
                     /translation="MLELLEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQVEIDKAVKLNRLSKFNVLGNAF"
     gene            12343..12396
                     /locus_tag="IW774_000867"
                     /pseudo
     CDS             12343..12396
                     /locus_tag="IW774_000867"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12400..12999
                     /locus_tag="IW774_000868"
     CDS             12400..12999
                     /locus_tag="IW774_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1153232.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGESIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            13015..13491
                     /locus_tag="IW774_000869"
     CDS             13015..13491
                     /locus_tag="IW774_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153233.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGMKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            13508..14410
                     /locus_tag="IW774_000870"
     CDS             13508..14410
                     /locus_tag="IW774_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153234.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQANNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            14483..14674
                     /locus_tag="IW774_000871"
     CDS             14483..14674
                     /locus_tag="IW774_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153235.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            14838..15425
                     /locus_tag="IW774_000872"
     CDS             14838..15425
                     /locus_tag="IW774_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153236.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            15425..16324
                     /locus_tag="IW774_000873"
     CDS             15425..16324
                     /locus_tag="IW774_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAQ1153237.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVSDATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            16324..17238
                     /locus_tag="IW774_000874"
     CDS             16324..17238
                     /locus_tag="IW774_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153238.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            17290..17505
                     /locus_tag="IW774_000875"
     CDS             17290..17505
                     /locus_tag="IW774_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153239.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NPVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            17696..17950
                     /locus_tag="IW774_000876"
     CDS             17696..17950
                     /locus_tag="IW774_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAQ1153240.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            18128..19171
                     /locus_tag="IW774_000877"
     CDS             18128..19171
                     /locus_tag="IW774_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAQ1153241.1"
                     /translation="MKKILVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTIDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNIISRQGWKIEGTGWYAMPNS
                     APNPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPRAN
                     DNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAGTYE
                     KSFNVTGMAVAVR"
     gene            19347..19577
                     /locus_tag="IW774_000878"
     CDS             19347..19577
                     /locus_tag="IW774_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153242.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            19580..19837
                     /locus_tag="IW774_000879"
     CDS             19580..19837
                     /locus_tag="IW774_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153243.1"
                     /translation="MTNEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEEIMKREGFQTDNDSKKVE"
     gene            19952..20923
                     /locus_tag="IW774_000880"
     CDS             19952..20923
                     /locus_tag="IW774_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153244.1"
                     /translation="MKTKGFNVKENAIGGNKIFKKDIYVWFNGKKYMTTNKRWSELVQ
                     NTVKKVMPDGYHFNYIQCSQTTESIYFSILNENRLYYFRVSSHHRKKREDNVTTFLSG
                     KYNGFKELSEAIGEYLKSNRFVRISDKHILILLLYLNNSFKLFWRDDTFYYIENGMDI
                     YSGINGTLKHYIKALLRYGVLYQEDLSNQIKISNSGKSLLAKYKKLLSPIYKGIDMEA
                     VNSETLFNLVVDEKFANEFFKSKKIESLNNLFPENAIKAYVDNNKSRLYNWFIPKRLR
                     GKIKIGDQVKVINLNHTKIVTVTELYIAEKEEITKMKPVIKILDNKE"
     gene            20933..21727
                     /locus_tag="IW774_000881"
     CDS             20933..21727
                     /locus_tag="IW774_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153245.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLGLAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMDKYRV"
     gene            21756..22874
                     /locus_tag="IW774_000882"
     CDS             21756..22874
                     /locus_tag="IW774_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153246.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKKTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETNTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            22907..23227
                     /locus_tag="IW774_000883"
     CDS             22907..23227
                     /locus_tag="IW774_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153247.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
BASE COUNT         8981 a         3192 c         4813 g         6523 t
ORIGIN      
        1 gcagatgcag aattcacctt aacggatgaa aaaggtgtaa aagaagttaa aaagacggat
       61 gcaactggtc tggctacgtt caacattatt gcgaacaaaa cttacaactt agaagaaaca
      121 aaagtaccaa caggttacac aggcgcattc aaacaagaag gaattaccgt caaagatgat
      181 ggacaagtct ttgaatatac agctaaaaac actttgaaaa aaggtgtgat caaagttcat
      241 aaagtggatg atcaagggaa agcattagca aacgcagaat tcactttgac ggacgacaaa
      301 ggtgtaaaag ttgtacaaaa aacggacagc aatggcttgg caacatttaa tattgttgca
      361 aacaaaacgt acaaaatcga agaaacacaa aaccctaatg gttacacagg aaaatggtct
      421 aaagaaggca tcactgttgc tgacgatgga caaatttttg aatatacggt aattaaccat
      481 cgtgtacctg aaacagtccg cacaggagga atcggtacat cagcttttct tcttgtattt
      541 ctagttgcag gattgggcat ggggttgttg tattatcgag aacaacgcaa agtaaaataa
      601 aaaatcggta atggaaaaga agtaaatatg aaaaaagaag gctgacgtat gaaatcgaaa
      661 aaaatttttt caaatctaat gctatttggt acattgctag gagtgggatt gggcgcaagc
      721 tcaatctctt ctagttttgg acttggggag caagtagcat atgcacaaca aaactataat
      781 tatcaaggaa ctggaactat tcatatccat aaagtaacaa atactaatgg ggatgggaaa
      841 cctgtctcag atattgataa caaagggaaa gaaatagctt taccaaatgg tgtacaacct
      901 cttgaaggcg ttgagttctc ttatgtgaaa cttactaaag atgaagctaa atccttggga
      961 tggacagata gtgatactac aaaaggtcaa catattgatg atgctaaagc acgattatta
     1021 ctaaacaaaa aatacagtga agtaagtaga atgaagactg attcacaagg gaatatagac
     1081 atacctgtac aagtaaatac agacgattta acagataact tgtatttgat tgttgaaaca
     1141 ggaacagtca aaggtgtagt tgaagaagcg tatcctatga ttgttaacgt acctgaacta
     1201 aacggatcag atgatcaata ctttaattat gatgtacatg tctatcctaa aaactatttc
     1261 caattaaact cagcagaact tgataaaaaa gacgaagatg gaaaggctct tgccggaact
     1321 gtttttgaat tgcgaaaaga tgatgggtct gatcaggtcg ttggaactta taaaacagat
     1381 agcactggta aattaactgt agacggtttg gaattaggtg actattattt cctagaaaca
     1441 gatttgggaa cggatgctaa taaaacaacc tatttactag atggaaaaaa attcaaattt
     1501 agcgttaaac gtggggagac tgctaaagta gttcttgatg ttaccaacta taaacgtcct
     1561 gtaattacta aggtgattga taaagaaact gttgatactg gtgaagatgt tcagtataca
     1621 ttgaaaccat ctattcctgg taatattgga gattacgaaa aatacgttgt caaagatgta
     1681 ctaggagaag agttgactta tgattcttca gaagtgatag aaggaatgac tttagatact
     1741 gactacagca tcgagcgtga cggacaaacg acaaactatg tctttactga ttctggtatg
     1801 gataaacttc agaaaaaatt tgatgcaggg gatcgcttct ttacaatctt gatgaatgct
     1861 cacacgaata gtaaagctaa aatggataca ccacttaaaa atgacgtttc aacaatctac
     1921 aacaatggat atgagcctga agacgaaacg ccagttgatc atgcttatac aatcattggt
     1981 gaccaccaat tcattaaaac agatggggat acaagcaaag cgttgagtgg agcgaaattc
     2041 gtagtgaaga acaaaattga tcctaacaga gttacttacc tgaaacaaga cccaacaacg
     2101 aaagctatta cttgggttga agataaatcg aaagctactg aattcgtatc tggtacagac
     2161 ggtaagttcc aagttgaagg tttgcaatat acctatgatg tagattttga taaacaaggt
     2221 aatccaacaa ttacagataa aaaaatcaat gattatgctc ttgaagaagt tgttgctcct
     2281 tctggatata caccaacacg aaatgatgtt ccatttgtag tatctaatga tgatgcttca
     2341 agtaaagtga cagacataaa aaacctgaaa actcctgaaa cagttcgtac aggaggaatt
     2401 ggaacagtag tattcctagt ggttgcaggt gtcgcaggaa cagggttgta tgttgtttac
     2461 aaacgggaac aacataaagc ttaattatta aaaatcccct agagtgtttt tggctctagg
     2521 gtttcttttt aaaaagagtg tgaaaagtag gtatgagtat gaaaaaaaag aatatcaagc
     2581 taatcatctt aagaattggg atgttgctat gtctaggagt aggtctatac cctgtaatta
     2641 ctcaggctta tacctattac cagactcaac aaacgttaaa tgcctataag gaaaaatatg
     2701 atgaagcttc tgaaaaggag aatgaagaaa tcttcaaaaa atattcggaa atcaatagga
     2761 aacgaaaaga aacagaacaa accttagtta cgattcctga agataataac aatattccgt
     2821 caaatggaaa tcttcaaaag gataaattgg gtgaattagt aggtgagatt cttataccaa
     2881 aaataaatgt ggatttaccg atttataatg gcgtaaacga ttttcaatta tcagcaggag
     2941 caggaatact tactggaact gattatccga ccggtgggat tggaaaccat tctgtcatat
     3001 cagctcattc aggtattcct aataaaaaat attttacaga tttagaattg ctcaaacttg
     3061 gtgataaatt ctttatcaat gttgcaggaa aaaaacttgc ttataaagta gatcggataa
     3121 aaacaattct acctaccgaa tttgaggatt tgcttcctgt agcagggaaa gattatgtaa
     3181 cgcttatgac gtgtactcct gttggagaaa attctcatag gttacttgtt agaggagtta
     3241 gaatacccta caaaaaaaat attgttgaga aagaaaaaaa gaaagaagac ttaaaagtaa
     3301 aggcgcagat aaactggtat cccatcatag ttgtaggtgt tttatcaata atagctttaa
     3361 tagtcattat taaaaaaata gtggcaaaga gaagagggat gaaaaatgaa gaataaaata
     3421 ctactttttt tgactcttat ttgcactttg gttttaacaa tttatcctgc aaatcagctc
     3481 tatcatcaat ttaaaacgaa agagaatgtg gcggaaatcc aagagaaaat tgataagaag
     3541 caaactcctt caaaagaaga agtagcaaaa aatgtcggac aaatactatc gaataacgag
     3601 aaccaaaaag taaacgaaac acaggagtca gataacgaat catttgatat ggatgaagca
     3661 gaaggaaaag tcaatccata tacaggagat gttttttcca agaaccaaac gaaagatttt
     3721 gaaccatcta cctattcagg agcggttggc tatatatata ttccaaagat caatgtgcta
     3781 gagcttttgt ttctaggcgc ttcacaggaa catttatatc gaggtgtggc gcaaatgagt
     3841 gctactagcc taccgttatc aggtaaaggg catcaaacag taatagcagg acatcgtgga
     3901 ggttatacgt caccacaatt tctttacgtt gatcagttag tagcaggaga tcacatttat
     3961 atagattatc tcggcgaaaa agcagtctat acagtttttc gatcagatgt tattaaaaat
     4021 tacggtcaga attcaacgct gactgatatt cctgatttgg atatgttaac actgtatact
     4081 tgtcaccctt ttcctcaaaa ctatgatcgt ttattagtca gaagcctaag agatgataaa
     4141 gcaaaataca tcacgccaca agtaacagtt gggaataccg catatgctga tgccattgat
     4201 agtgaatccg tttcagctaa gccagaaact gttacatctt ctaatgattt atttgaggat
     4261 gttgatcgtg caaacaagga attagatgga aataatgatc ttaatattcc ggaacaaact
     4321 agcgtagaga tgcaagacag aacgtttgct gaaaaggcaa gttcaatatt tacaggaatt
     4381 accaataaac atcagaaaat gcttttagtc atttggactg tattaggagc attagcaagt
     4441 ctaaaggttc ttcttctttt aatcaaagaa tttagaaaag cataatgaag attataaaaa
     4501 ttgatagagt acagtaaaaa ggtattgata ctattattga tctctttttt attttgaata
     4561 gagctagatt cacttttaaa taaaatcaaa gctgatctta attccaaaga aaaagtcaag
     4621 aaaaaaataa aacaattttt ttaatcagaa tatttatcag aaaattatta gaaaagataa
     4681 tcttaaaagc ttaatttaat aggattattg gttgtattta aaaataatgt atggaaaagc
     4741 tataaatcaa tagataagat gcagaaaatc ttatttgtga atgaaaaaca agtcatcgta
     4801 aaatattaat aggaggtgta gtaatggtgg taatatcaaa aacagcatta gactcattag
     4861 ctaaagtatt tagtagcagg tttgatggtg cagaatttgt tgaatatcta agccaaaacg
     4921 aaagtggaaa aactctgatc gaaacactga aaaaagacta tttgaatgaa aatgactttc
     4981 cgacgtggac gaatattgaa gatgctacat gggatgtagt gtgtacgata tattctcctt
     5041 gggtaacgat tgcagatttt gttttagata aaattggagg ggatacatac cctgatgcca
     5101 aatctcttgc tacaagtgac tttaaaacgt tctatggtta tctaatgggt ctaataggca
     5161 atggaataga tttgaaagtt acgccaaaac aaacacctga agaaattgaa aaagaaaaag
     5221 gagaggttca aatggcttat gcagatgaca gagaaaaagt aaaattattg gaaggtaaat
     5281 tatctgaaga agaacttgga aaaatggtgc aagaaatcaa tgaagacagc aactattttc
     5341 ctgaattgaa ggtatatcaa aacgacgaag atttctttaa tgaaatgttt agtaatagtc
     5401 catgggaagc agtgagtaaa acaaatttag gagaatggaa ctttcaagat gatctagttg
     5461 catttagcaa tgatgggtat ttacgatcat attcagaaag tgagtataga gctgaattaa
     5521 aaacgtatga aacggatatt ctgatgagct atttaaatct attagagagt ggtcagggag
     5581 agttaagaga taaagttgaa gaactattaa aagaagagct atagggatta tttcaacata
     5641 aacacaagca gaaaactacc aaacaaaacg taccatgcaa aaagccggtt aaaccggctt
     5701 tttgctctat tatgagagga gatcaaacca tgagtgcaac aaccgatatg gctttagaag
     5761 cagtaataaa agataataca ggaaatgtcg aaaatgtaga agactttagc gatattgcaa
     5821 tcaatctttc agcgtggatt tcagctgtag gagaagaaaa tatcgaattt gagatcaaga
     5881 atatggatga cttaaaagca aaatttcctt ttatcgcatc gaaactaagt caatttgccg
     5941 aggagaaatc gtggaatgaa ttagttagtt tttccgaaaa aatttatgat attacgcaag
     6001 aagatgagtg tgtttcatgg gaaatgaata atcaatacaa gaaaattgat gctttcgtca
     6061 aatcacgtat agaaggaaca gcttttgagt ttgaaggtat agataccgca gataattggt
     6121 taaataaaga atcctttatt aaaagtatag cggatgatct tgaaaaacaa tggtctaagg
     6181 aagactatgc agagtaccta aaaacagaaa gagatacaga ggtgaaagaa aaactcactg
     6241 aggtatgttt ggatgaacat attgatccca atatcgtaaa acaagaagac tatgaagtgg
     6301 ttattgataa agttcctact gattttgagg aagttgtaaa gtatatagtt gaagaagcat
     6361 tatcgcaata ctcttcagat gaggaagtgg acgaagaaac tgttcgtacc agagtttggc
     6421 aactggtttt tgatgacaaa gattataaat atttgagtga tcaaaactcg gttaattgga
     6481 gtgtacgatt attaattgat caaaatgata ttgaataaaa tagagctgaa aagctctatt
     6541 ttttttgata aaaaaactag ctattttttt aaaaataatt tggttcaaaa tttatttttt
     6601 taacttgata attttttgcc atcctttttt aaattggcta tatatcaagg tttatagtgg
     6661 ggataaataa aaatacattt gacaaaattt acaatatagt ttattatata tttatcttat
     6721 tatgataaca caataaaagg aatgatggta tgaaaaagaa gaagtttagt cgatcgttgt
     6781 taggaatttt agttttagtt gcggttggtc ttagtggcaa gggttacttt cagaataaca
     6841 gcactcagga acatgatttt cagatcaaaa gtagtgttca ggtaggtaat aaagcagaca
     6901 gtgaatcaaa agaattttta gggatacgtt atgatccaaa caaatatcca aataatatcg
     6961 taaacattaa tgatgggaaa tatacactga caaaagaaga ccatgaagca ttatcagaaa
     7021 aaggcagtca aaccttttgg gtaaattatg cagatttaga tcaacttgga agagcaggag
     7081 atgttgaagc gttgatcacc tatgatgctg taaattctca ctcatctaaa aatgtggaac
     7141 gtccatcttt caaaagttct gtccatattg caggagaata caaggatgga gtatttgatc
     7201 aagagagaga tacttgggtc ggcaaagatt caaacaatga aatcactcag ctagaaggat
     7261 ataaaggtta tctctataac aaaagtcatt cattagcatg gtcgctagga ggagatatgg
     7321 aggtacacaa tttaacactt ggaacgagag cgcaaaatgt tgggaaaaat gatcagaatg
     7381 gagggatggc ttactcagaa acgattgtac gtgatgcaat ctataaaaat aagaatgaaa
     7441 aagtattgta taaagttgat ccaatttata aagggaatga attggttcct cgtggttctc
     7501 atgtcaaagc tttctcagtt aacgataatg gaaaaacggt aaaccttaat gtttatgtat
     7561 ttaacaatca aaaaggaatc aacatcaact atcaaaatgg tacttgggta agagattaga
     7621 aattgagaag gtgaagaatt ggaaacaata agcatcaatg gattagataa atcagttgca
     7681 aatcgaataa aaaaaggaaa tggctactat cttctgaaga agggtaaata ttcagttgct
     7741 agagagttag aaacaatgag tatcgtgtat gtagctaaga atcattttga cgtagaacag
     7801 gctatttttg atccagattc tgaaataggg aaatgtgttg aattatcaga gaaaactgtt
     7861 agtacaaaag gggcgatgat cattgagaga gaaatgttag acgaaatcag agtaatcatt
     7921 cctaaagata cgcagatgtg tgaggacagc ataagtgacg atgaaccaaa atcagaaaga
     7981 aaataaggag atattcagat gagcgaaaaa tccattgaat taaaggaata taagcgaagc
     8041 gatggagaaa aatattatcc tgtagccagt ttttctaaat cagtatatct ggttggacgt
     8101 gcattggtag ttggtgcaat tggcatatat attttgcatt gggtaacaaa ggttgtacaa
     8161 ctgcttgtag ataccttagt aagtattctt gtctcgttaa atgatgttaa cggaacagat
     8221 gatttaggat taaaagaaat gtttaaagag ttactcaatg cgttttttcc caattattta
     8281 agttattcat ataatgtaca aagctttttt gcaaagctac acttggtaga catctctatt
     8341 ttagtattag aagcaggaac ggtattcttt gttggatatt tggttacttt gatgttcagg
     8401 catcactatg gagaacaaag accatttttt gatgatatgg aaagtagaag gttgaaaaga
     8461 gaagctctag aagctatgga tgtgaagtat gatccaaaca agattatagg agatgaaacg
     8521 aacaacaaaa aaagaaaacg aaacaaaacg caattagaat ccgatgtgct atcaacgctt
     8581 aaaaatatca aaatttctgt tcatacaaga cgtgatctaa gtgtcaatga agacactcga
     8641 gaatatatcg taaaaatccc gcaaccaaaa gggaagaaag ttagagaaac agttttacga
     8701 caaattcaag atttagggtc gatcctgaca agagcaacaa gcggacgagc caaatttggt
     8761 gaaaaagaag atgaagtttc aggcgattat ttcattttcc gagcatcagt aaatattgaa
     8821 agagaacgta aggaaagagt aagtaccaaa aacaaagaaa cggaaagagc aacatcttca
     8881 atcgcagttt caaattcaca attttctttt ccaatcaatc tctttgccga taattccaaa
     8941 aatatccaaa accaaaccga aaaagctaga aaatatgccg aaactcaaaa gcaatcttta
     9001 gaactttatc tatcttctgc tgacgtacca gcaagtctaa cagatatgca cgttgggaat
     9061 acatctattg aatatttgtt tactttatct cgtaccagaa aaattcaact aagctcaatg
     9121 aaagaagaaa ttgaaactta cttgcatata tcaggtgtaa tcgtgcggat caacgcaggg
     9181 cgtattcaaa ttactttacc attaggagaa aatgatcgta ttccaattga tgtaccatca
     9241 atgatcaata gcgtgtttta gaagacatca caaatagaaa cagaaaatac aaactaccag
     9301 ttcacaaaca gaaaagtacc gagaaaagtg aggaatagaa atggctatga aattattaga
     9361 gaaagtcaaa aagagagccg aaaaagctga atctattttg ctagataaag aacctaatat
     9421 aaagcttaat ggaaaattca gtgtcaatat gttgaacaca aaggctaaag atccgacaga
     9481 tgctattttt ggaattaagc ctgatggcac aattgctcat ttttgcatca gcgaaatgcc
     9541 acacatgttg gttgcaggaa cgacaggatc agggaaatca gtattactga atgaaattct
     9601 tgttacagca atgtgtcact caacgcctga tgaattaaaa ataggaattg tagacccaaa
     9661 aagggttgaa tttggaagat acaaaaaatt gccgttcatg ttggctgatc caattacaga
     9721 tatggatgaa gcttatgatt tttttgaata tcttgtgatc ttgatgcatg aacgttacaa
     9781 actgatggaa aaagcagggt tacaaaacat cacgctatat aacgaatatg ccgaaaaaaa
     9841 tggattagat agatttccct atgtgatctt attagttgat gaatattcac agttggttgg
     9901 aacacataaa gaagtagaag gtttgattgt tcaattagga caaatggcaa gggcagcagg
     9961 tattcatgtt atcttagcta cacaatctcc tcgatcaaca gttgtcactg gtattatcaa
    10021 agccaatttt ccgtcacgtg tttgccttat ggtagcaagt gatttagaaa gtaggatcgt
    10081 attagatgaa ggcgatggag aaagcctaag tccaaaggga gatatgatta ttaagctcgt
    10141 taatggaagt atggttagag cgcagggagc ttacatcagt aacaaagaaa ttgaaacaat
    10201 tttcaatcac cttagaaata ctatgccaga accagaatat gttgattacc gagcaatcgt
    10261 agctgaatct cgtggtgagg gtggagaaga cgaggatttt ggtggaattg gtaaagctcc
    10321 tgtaagaaaa tctgctaaaa gaaatttgaa cacaccttta gcaaattcat catctaaaga
    10381 gccatcaatt gaaaagaaga gtacgtttgc tctaaaactt gaaaaaaaca aagaagaaaa
    10441 agaaaaacaa ggagctaaaa cactagaaaa acttcaagcc gaaaaattag aaaaagtttt
    10501 aaaagcatta gataaagctg attctgaacc gcaaacctca aacaacacga ataatgaaca
    10561 gccgatttca tctaaaacta aaccgaaaac tactgatccg aaagtttcag taaacgtgac
    10621 catcacaacc gataagtctg agaagaaaca agtaaacgga acgacttcac ctatcatgca
    10681 aaaaatgttg aaagccaaaa aacaggtgca taaatgatca ttgtcagttc ttttttgagg
    10741 tggcttacgc catataaata tacagatgaa gtagttgtga aacgaacgaa gaaaagagat
    10801 aaaaaagcgg tactaactcc taaacagatg cttgatcatg gcattttagg ggtgaaggtt
    10861 ttagaagtga ataaaccgtt tgaatcaaat agtgcaagag aaaagttgct tggagctgaa
    10921 gccgatgtcg catattttta ccatgacaaa agcaagcatc aaatagctat taaattcaat
    10981 ccaaaatcgg catggcggta tcaagcaaga gatgacttga aacgtaggaa acttagacca
    11041 atgctctatc cagaaggaaa agaagcattt gatagaaccc acatcgttcc cattggtttt
    11101 catggtacag aatcagataa ccgcctatta gtaggttgga acagcaatca aaatagagtt
    11161 cagatcaata aatttgagaa gaaagtaagg aaattcaacg atcgaaaaac gatcctatgg
    11221 tttgcagata tttgtaaaca gccggatgga acagcaaagt gggaagcggt gatatttgac
    11281 gaaaatggta aaaaagtaat gtctgaaaca tggcatgata aagctaaatt tgtttggcag
    11341 tagcagtaga ggtgaaaaag attgctagaa cttcttgaag caaatagaac aggctttaat
    11401 caaaaattag aagtgggaga aactatagaa tataaagata aaaactatat tattatcgga
    11461 atctataaca cgagaattaa cagaataggt gatccacgta taaccagtga cttggtttgt
    11521 caatctgtta actattcacc tgatctgaca agcaggtata agaaatacgt gttattagaa
    11581 tatagacaca agattactga tgaaattggt gtaaacaaca cacgaaacat atttaagatc
    11641 ggaacagtga ttccttatag taacaacgaa gaaaaaatat tttatcagat ttacggaata
    11701 gaaaagtttg aatatgaaca tgtcgatctg ctagtgacgt accaaatgcg tttaatagaa
    11761 ccttggtcac aggtagaaat tgataaagca gtaaaattga accgattatc aaaatttaac
    11821 gtattaggaa atgcctttta aaaatcgcaa tacacaaaca gaaaaatacc aaaaaattag
    11881 caatgaacgt aggtaagaac atgacaataa ataataataa ccctaaaaaa tataatgctc
    11941 ttgtccaatg ctatatcgaa aacaaaatta caatgcaaga agtcgctgat gaattaggca
    12001 aatcaaagag cctagtatca cacgatttta agcacaatgt ttttccgaac ccgattgagc
    12061 tgaggaaaaa atttcaaaag agagtttatc aagaaatagg taagtggatt gatgaaggga
    12121 atgcaattga ccctttaatt gaaaaatatc cttttttaaa aagcaaaaat aaaattcaaa
    12181 ggttaattcg tcaaggcgat attcaagcta aagaacctgt tttgacaacc aatgatttgg
    12241 ctaaaataaa tcttcataaa caaattattg aagactttga aaaaaatctt tcaacaactg
    12301 aaattttaaa aaaatataat attccgagag gaaagctaac atcgactctt tttagagaat
    12361 atggagaatc ggcagttact aaagcaaagg aagtagcgaa tgagagaaaa aaacaaaaat
    12421 agtagtcgat acctagtagc gactgtagtt tttgtttgtg tattgatttt cctgtttgat
    12481 tttttgcaga ttggagaaag cataattgga aatacattga gtgcaaagga aatggttgct
    12541 ttaggtggtt tgtatgaagg acacttgttc ccgaatatat tcatttctat cgtttctcat
    12601 attaacttgt accatttaat ttctaattta ttgttcttag tagttcttga agaagcgctg
    12661 aatgatcagt atgagtggtt aacattattg atcgttttaa tcatatcagg atttctagga
    12721 agcttagttc cttttctgct acacagcaac atcgtaagta tggggcttag tggaggtatt
    12781 tttggatgga ttgctttatt tgcgattgca tacatccgtt ttgatagcaa gaaacattta
    12841 ttactaagtg tttttgtatt attgataatc ggcattttac tggtatctac ttttgaatca
    12901 ggcgagacaa acgtcttgtc acatgtaatc ggatttgtct gtgggatgat cttgggattt
    12961 attctaaaga acgtttcatt gatcaaacaa aagaattaaa agggagtgct aaaaatgcca
    13021 aaaaaaaaag aaaagaaaat tccagtgttt ttgtctgtct cagaaatgtt atcacttcaa
    13081 atactgttaa gaaaaaagat tgaaacaata gacgaagaag ttagaaataa aagggctatg
    13141 tacgaacaac gtgaagcgct aagagaacga atccactatt ttgatgatat acacactaaa
    13201 atctatgaag cacgtacaaa tcacgaagac gaaagcaaat taggattagc tcaacaacga
    13261 atatttgaat ggctgaatac gaatactgag caagaaccgt taagagcaat tagtaatctt
    13321 tttgaatttg gaatgaagca ttggtttaac gctgtagaaa aggatgctga cttacaagaa
    13381 gcgttcgata cactaagcga ggatagcaaa tccatgcgag acttattgat cacttatcta
    13441 aatgagcatg aatttgaaca gacgaaagtt aaaaagaaaa ataaaaaata gaaagttgtg
    13501 atgtatcatg ctgtttacct tattaggtta tttatgttta gttcttgctt tttttggagc
    13561 aattgctata tttgtcggtc agtttcaagc aaataatccg ataatgcgta acataggcat
    13621 tggaattgtt atcatatcct tgattgtatt tggtgccaca aagatttttg ggagtaatga
    13681 tcagcagttt ggtatttctg gagttgataa tagttatgct ccagattcat tgggaagagt
    13741 agtgatagat ggaacgtttg agaatgtaac gagctacaaa aatgtttcat tgacagtgaa
    13801 agaagatgac aagaaagcat cagatgacgt tctcgctacg atcaagccgt ctcaatcaca
    13861 gtttgaagtg ttttatacag tcaaagagaa taaccctgta cgcaagctaa cattagtttt
    13921 taacgttgac ggagaagaat actataaaga tgtaaaaatc aataaagcat caacgaaaaa
    13981 gattgtcgaa tcagcgaaaa aagaagtagc tacagaagaa agtacaactg aatcaagttc
    14041 tactgtcgaa gttgaactac cagataaaat tgattttgat cctgaaaaag tcaaatcgtt
    14101 tgaatctgtt tctggttcta tggcagatcg gtttaacact gaagttttca aagctatcaa
    14161 aactgttaaa ttggatactg aaaagcaaag gattaatgtt accttattca attcagttga
    14221 atctatggat caggataaaa aagttacggt tgcaagagaa ctaggaaaaa gagtaattgg
    14281 tttgtatcgt ggtactgcta aaccagattc agacggtgca gatttaccag ttcatgtagt
    14341 ttatgaagac ggtaaagttt ttggagattc tacagcaaac gaagcattaa cgattaaatt
    14401 ggctaaatag ccagaaagtt ataataaaca aatggtgtca caccaataat atataggtgt
    14461 gacaccttgg agatgatagc tgatggtagt aaataaagaa aaagttagaa agtatgtgac
    14521 tttaaaaata gcttctgtta aatatttaga aaagcgagct aaagaagaag aaagagcaag
    14581 aggtaagaaa gtaacagttt cagagttgtt agaagaattg atcgagaatg atcaaacaat
    14641 aagacgtgca tttactaata ggtctatcag ataacaatat aattaataaa aaagaaagag
    14701 tgatttggtc ttagactaaa tcactctttt tatataaagt ttctttacac ttattccgaa
    14761 taatgttaaa attctttata cgatatatag ttacttgtca gagaaagaga taaccagcaa
    14821 tattggaggg ataataagtg gaaaacaata atgatattag acattttagc attggggaaa
    14881 gagttacaac agtaagtgaa ttatcaaagg attatagaga taaagaggga acgattattt
    14941 ctcttaataa gaatcgaaga ggcgtttggc aatatgttgt tgatttaggt gatggcgtta
    15001 cagttgagtg taaagatagt caactgatca gaaatgatgg cagtcattcc attgaaaaag
    15061 caattcttgt atacccaaaa gtatcaagta agatcatgaa tgactggttg cttggaaaat
    15121 ttggtgagta caataaaaaa gaaatcgcag aagtgatcgg caaatcttat ccaacagtat
    15181 tagggtatct aaaaaagata actaacaaag aagcgaaagt tgaggatgtt ttaacagtaa
    15241 gagaacttca gtcattaatc gaatatcgga ataaacaaat aacaaatcct gagtacctta
    15301 aaaaagaaga tggtctattg aaacaattgg aacaggtaaa taaaatccgg aagatgcaaa
    15361 aggtagctcc tttaagccta gataacttta aaaaaattta tttgaaagag atcgaaaaag
    15421 actaatgtgg agtgtcactt taccgtttca acctgtatct gcaccaagac caagtgcaag
    15481 aaggaatgtt tctgatgcaa ctaatgacgt agcagagaaa aggatttcaa cctattatga
    15541 taaaaagtat ctcgattatt tgaaaaaaat aaaaagcttt atcgaagatc aaggtttgtt
    15601 agatgatgaa ttttactcga tcgtaaatga tccgatgggt gcaattatgg aagtggactt
    15661 ttactatcag ataccaaaaa gctataaaaa agtctataac attatgaaga cgacagcgcc
    15721 agacttagat aatttagtaa aaagtgctat ggatggtatt ttcaacatct cagctatacg
    15781 agatagccat atcacaggct taattgcttt taaatataat gtggcgaacg aaggaaaaac
    15841 agttgtgagg atcaaacgat attcagaatt tgagtgggat acttctgaag gattgaacgg
    15901 agatatatgg gaagctacat ttgatttcaa accagtagca acgccaagac caaagtctaa
    15961 atttagagga aatgggatta tcacttacta tccaaaagag tacaacgact atttggaaaa
    16021 tatcaagaat acgcttaaag agaaagattt ggtaaatgat caactgaaaa aggtaatgaa
    16081 tgctcctatg ggggttatag ctcagataga ttatttttac caagtgagaa aagataagaa
    16141 aaagcttgat tcactgatgc gtacttcaca gccggatatt gataatttag tcaaaggaac
    16201 attggacgga atttttaaca aggaaatagg catcaaagat agcagggttg tagggttaat
    16261 tgcttttaaa tacaatactt ttgaaaagtc gaaaacaaac gtgagactac aggagatggg
    16321 ctgatgaata ataatgaaaa aattcttcat gtacttgata gctttgaaac aattcaagaa
    16381 gaactaaaga aatatcggga tgttttggaa caaagatatg attttgtcaa tcagcaaaag
    16441 agtaatcaca tggatttcat tctcaatatg aatgacttga agaagaaatt agttgaacga
    16501 aaagaacaag aaaagttgat caaagcttac tttgaactcg gtgaaaaaga agttaaaaag
    16561 gcaatggaac tgaatgaaga tcgcagggtt ttagatcagc tgttagaaca attattggtg
    16621 atgtttcaaa aaggacgaat agatgaggat ttaattgaag aagggttgag aaagtatcct
    16681 tcaaactcag gtatcggaat agtgctgaaa gcgattgatg aagaagaaat tgaagctttc
    16741 ataccgccag aggattttga atcggcaatg gaatatataa aatattatag ccaagggatc
    16801 actgctttta gagagtttga tcctgaagat gttattcatg acttgaacaa cctaaaagaa
    16861 tggtgtgaaa gttatgaagt cgatgattcc ggattggact acttgatcag tattatggaa
    16921 atcgaagaag agatgccgga taagcctgat ccaacagata ttttagaatt gatccatgaa
    16981 gctagaaatc caattgccta tatttctaga ggatatactg tattggaata ctataagcct
    17041 tatatatcag cgatgaacca tttaagacgt gtattaaggg aaaaacgtga ataccgctca
    17101 gtgttgaatg caacgaatag attagaaaaa gctgtttcag agctagatgc ttattatcgt
    17161 gaacattacc tgcaagcagg tggtatgcca agaaatacga aagcaaatat cagtaggtat
    17221 ataaaaaaag ctgagtaggc ggagttagcc cgtaaaatgg caaaaggtaa cctgaaaggg
    17281 gcattttgaa tgaaaaggga tgtttttgaa ttgtacgtat tagaacgtct agaagacggc
    17341 atgatggcac aagagataat tgatgaaatt gatgatatga tcagcagatt tggtcaattg
    17401 gagttgaatg acggaaacaa aaatccggta gtgctaaaac aattttggtt gaacaatttt
    17461 tcgtataagg attttatcaa agaaaaggca aatacattgt actgactaaa ttcacgagga
    17521 gcagtgatga gagcttattg ctaagagcag ttaggctctt ttttttgttt gtgtaaaatt
    17581 atttgtttta caatacacat ttatacacaa aaaatattga cggatagtac acaaatgtgg
    17641 taggatatat ttgtaagcaa gttagattaa caagttaaag gaaggaagag cagttatgaa
    17701 aaaagaagaa ctaatagata tatttcaaat tgttgaacga gcaaataata tgggaattat
    17761 gttttttgat cgtatttctt taaagatgga tttaagcgtt gcccaccaag aatttaatct
    17821 acgattaaaa gccttgttga attctgatga tgttaatttc gctcatgatg ttgtaggcat
    17881 acagaaccat atagatagag aaaacaaacg tatgggtgat ggattcttac caagatattc
    17941 aagtttataa tttgaagaaa gattcaagag tatgtgtcat tattgatagg tactcttgaa
    18001 ttggaaagga ggaatgaatt taagacaaca acgattatga tcttaaaaca atcgtttatg
    18061 taattgacaa tcttacgaat aattttcatg caataatgac aataaaaatg aaaaggggta
    18121 aaatcaaatg aaaaaaattt tagtaggaat tctattcaca atggggctag tgctattatc
    18181 accttcagtt tcagcagaaa gctctatttc catgtatcgt atgtacaatc ccaatagcgg
    18241 agagcatcta tacactagaa gcgttggaga aagagacaat ctgaaacgtt atggatggca
    18301 ttatgaaggt gtcgcatggc aagctccaac aagcggtact cctgtttatc gtttgtataa
    18361 tcggtataac ggtgaacatt tttacaccat tgatgccgga gaaagagata agatttcgca
    18421 cacaggatgg acgtacgaag gtgttgcgtt ctattcctac aacggagcaa atagagttcc
    18481 tgtgacacgc ttgtacaaca aatacgtgaa ttggcatcat tacacgttag atcaaaatga
    18541 aaaaaatatc attagtcgac aagggtggaa aatcgaggga actggttggt atgcaatgcc
    18601 aaacagcgca ccaaatcctg cacctgctcc aagcaatgaa aaagtgttaa atgttcctgt
    18661 catttggcaa ggtaatacga tgttgtgtga aggtgcttcc ctgttagagg gattgcacta
    18721 caaaggagta gcgacaaatc aaaacttaac cagttttgta aactatatgc caagagctaa
    18781 tgacaataat ccatatcatg gctattctgg cgaatggcgc tacaacgtaa atggtacgta
    18841 tcaaggcatg atgcctgatc cagtagttaa atgggcaaaa aatgttggtg gtaatgccgt
    18901 taatatcact ggttcaggaa cgaatgggat caagaatgaa attcgcaatg gtaatccggt
    18961 tgtaacttgg attacttata actatgcgac gccagagttc aaacaaatgc cttgggggcg
    19021 tgccgtttgg aatgggcatg ttgttttggt agacggattt aagaatggcg cttaccatgt
    19081 agtcgatcct gtatttggag caaaatggat caatgcaggc acgtatgaaa aatcgttcaa
    19141 tgtaactggc atggcggttg ctgtacgtta attgatagaa ttaataaata aatgaaataa
    19201 gagcttaatt actgaaatgg taaataggct cttgtttttt aggaaaaaac tgctttttgt
    19261 aataagaata tataacaaaa aaattgaatt tagtacacaa aagtggtata ttaaaaccgt
    19321 agagattatg cgaagaggta attttgatga accaaagaag aagaaaaaga ctagaagaaa
    19381 taagcgagcg actatccgag ttaaaggatg agttagaagt aattcaagaa gaagaggaag
    19441 agtacaaaga aaatatacct gaaaatctac agggatcaga aagatatgaa aaagcagaag
    19501 aatccgttga tcaacttgaa gaagctgttg atggtttatc cgacatatta gaattgattg
    19561 aagatatagc tagctagaga tgactaatga agatttcagg agtgagcaaa aaatgattga
    19621 tatttcactg atagaaaagc tacttttttc aaataagtac acagccaaag agctggctac
    19681 aagatggcaa atatccgaat cagctattaa agcctatcgt caaaataagg gaagcgtagg
    19741 atggcgtgat tggaaaggaa tcagcattgg aaaagcagaa gagataatga aacgtgaagg
    19801 atttcaaact gacaatgatt ctaaaaaagt tgaatagttt aatagtcgcc aagaaaagta
    19861 ccagggcgaa aggtagatta caaaggaaaa cacttttaaa taaacttcgc aaaagcatca
    19921 ggttatgagt ggctagggct atacgaatga gatgaaaaca aagggcttta atgtcaaaga
    19981 aaacgcaata ggggggaata aaatttttaa aaaagatatt tatgtttggt ttaatggaaa
    20041 aaaatatatg actactaaca aaaggtggtc agagctagta caaaatacag ttaaaaaagt
    20101 catgccagat ggatatcatt tcaactatat acaatgctca caaacaactg aaagtattta
    20161 tttttcaata ttaaatgaga atagactcta ttatttcagg gtaagttcac accataggaa
    20221 aaagagggaa gataatgtta cgacattttt atcgggaaaa tacaatggtt tcaaagagtt
    20281 aagtgaagca attggtgagt atctaaaaag taatagattt gtcagaattt ctgacaaaca
    20341 tatattgatc ttgttacttt atttgaataa tagcttcaaa ttgttttgga gagacgacac
    20401 tttctactat atagagaacg gcatggatat ttattctggt atcaacggca cattaaaaca
    20461 ttatattaag gcgttacttc gatatggggt tctttatcaa gaagatttaa gtaaccaaat
    20521 taagattagt aattctggaa agtcattact ggcaaaatat aaaaaattgc tttctcctat
    20581 ctacaaagga atagatatgg aagctgtaaa ttctgaaact ttatttaatt tagttgttga
    20641 tgaaaagttt gcgaatgaat tttttaaaag taaaaaaatt gaaagcctaa ataacctgtt
    20701 tcctgaaaat gcaataaaag cttatgtaga taataataaa tcacgattat acaactggtt
    20761 tattcccaaa cggttgcgag gaaaaatcaa aattggcgat caagtgaaag taattaatct
    20821 aaatcatact aagatcgtta ccgtaacgga gttgtatata gctgaaaaag aagagattac
    20881 aaaaatgaaa ccagttatta aaatactaga taacaaagag tgagtggaat atatggtagc
    20941 aaaaaagaat actctaaaag aagcttctga aaaatatcaa gatatgaaaa gacgaggatt
    21001 aataccaaag gacaaaaaag gtatatggag agaatttgtt aaggaagtca gtagtgatcg
    21061 aagtggcggt gactttgttg gacgatatac agcaaaaggt tatagcaggc gcttaaggat
    21121 cactgttgaa tatggaacaa atgaatcagc tacagagctg aagcaaaggt acttagatac
    21181 acgaagagaa gcttcaaaaa aatatgatga tttcaagagc agaggtctaa ttcctaagaa
    21241 gaagaagggg atttggcgtg agtttttcaa agaagaagat agcaatcgga ataagggaga
    21301 cttcattgga cgatacacag caaaaggcta cactaagaat ttagggatca aggttgaata
    21361 cggaactggt gaaagttcat gggaattgag gaaccgctat ttaaatacat tattggagaa
    21421 atacaaagca gaattattag agctgttcga tgttaatgat ttcatagaga tggctgaaaa
    21481 atatggtcat gaagaaggct ttatgatcaa tgacattatg gttattgata attttacgga
    21541 cttattagga cttgcgaaaa aagcaagaga tttaagagaa acacagatgt atctattcag
    21601 aaataaaaac ggaagaaacc ggagaactga atcgagcgag atcgaagatt actatgatgc
    21661 ctttacacac gatctaggag cctttgtaga cgagatcagt ggtgttatgg acaaatatag
    21721 ggtctagaat aaaaaagccg gagatgagca aataaatggg aaataatagc atatatacac
    21781 ctgaaatgca gaataaaatt aaaagacttg tcggattagg gtggatgaca agaggtaagt
    21841 tagattcttt tagtacgatc aaatatcttt tgatggatat ctaccgccaa aacaagaaaa
    21901 caggaataca ttttttgggc tatgataagc cggaagatgc gcctttcttt gttcctagtt
    21961 cagcctatag agccagtact tccggtagca tgtataactt ggatttgttt gaggaaggag
    22021 caaagatgat cgaatatgat tttaacgaag cctattcgag gatcatgaga aattatcctt
    22081 tgccgtcaaa cacttatttg aaagcaaaat ataatacaga tcaagctatt ttgagagcga
    22141 taaatagacc aaaagtaaga ccgtatgaaa atttaaaaac gtatatgttc tttttggtta
    22201 attttgaagg aacagccaaa gaaggaacgt atgctaatcc aaaatcacag tcgatgtttg
    22261 cggattatgg cgaaaatccc aaaacagcta atgagcctat ttggctatcg gagattgagc
    22321 taacactaat ccttgattat tatgatatca aagagatgga agtcctcgat agtcacacat
    22381 ttttgacgaa aaaaggatta ctcaaagaat atttcgaaag aatcgaacca ttaaaaactg
    22441 atcctgatac gttgaaactt tataaaaatc ttagaacgaa agtatttggt gctatcggac
    22501 aaaaatcatt aagagaagga gaaacaaaca ccttcgataa atacagtatg tataatcgag
    22561 cgtattcctc catggtagca ggcgtattta gagatcgtat ggttaggtat gaacagaaat
    22621 acgttcatag cgcatatggg ctattaaaaa tccgtactga tggattgtat ttcagatccg
    22681 aagtaccaga gtttgaagag ctgtataaac ttggagtagt taagaaaaaa gttcatacaa
    22741 tatccaaaga agaagtcgaa aaagctttag aatttaaaaa caaaggattt gaaaataagg
    22801 gctcctttgg gaactctaaa gatgaagtta agggtgttgt gatagacgat gatttcgagt
    22861 ttgattttga ctaaatactc tttggaaaag atggtaggag gtaaatatga agaagaaaat
    22921 tgtggaagga aatgtaggtg ttattattct aaaagattca actgactatg ctaaatcaat
    22981 ggcactaaaa gacgaaaaag ttgcacgcat ggataaagat ttagttgaat ttctgatgga
    23041 tgatacagaa agattaaaag gggatcagtt tttgagttta atgaaagaca aaggcttaaa
    23101 cacatatggt ttatttgatt ataagaaatt agctatagag tgggtaaaag aaggagaaat
    23161 attttttatt gaagaatggg aaggtactga gaacttagtt cttcatcagt atcttttgaa
    23221 agcttaatct aagtaaaaca gttctgacta gttatgcttg tcagaatttc aaaggatgtt
    23281 ttccataacc atcatcattc aaaaataaag gatttgaaaa taagggttcc tttgggaaat
    23341 ctaaagatga agttaagggt gttgtgatag acgatgattt caagtttgat tttgactaga
    23401 ttttttgcaa tgtttaagag taaacaaggt aagaactaag atcatgaaga ttgagagtaa
    23461 agaagataga ggacgaatgt atgagaacac actatattta tttagaaga
//
LOCUS       DACEOE010000026        23154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEOE010000026 DACEOE010000000
VERSION     DACEOE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.027"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(110..181)
                     /locus_tag="IW774_000884"
     tRNA            complement(110..181)
                     /locus_tag="IW774_000884"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Arg,seq:ccg)
     gene            241..1575
                     /gene="rpoN"
                     /locus_tag="IW774_000885"
     CDS             241..1575
                     /gene="rpoN"
                     /locus_tag="IW774_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1153248.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            1778..2815
                     /locus_tag="IW774_000886"
     CDS             1778..2815
                     /locus_tag="IW774_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1153249.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            2856..3857
                     /gene="gap"
                     /locus_tag="IW774_000887"
     CDS             2856..3857
                     /gene="gap"
                     /locus_tag="IW774_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1153250.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            3967..5157
                     /locus_tag="IW774_000888"
     CDS             3967..5157
                     /locus_tag="IW774_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1153251.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            5261..6016
                     /locus_tag="IW774_000889"
     CDS             5261..6016
                     /locus_tag="IW774_000889"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1153252.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            6132..7430
                     /gene="eno"
                     /locus_tag="IW774_000890"
     CDS             6132..7430
                     /gene="eno"
                     /locus_tag="IW774_000890"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1153253.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            7829..8968
                     /locus_tag="IW774_000891"
     CDS             7829..8968
                     /locus_tag="IW774_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1153254.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(9035..9610)
                     /locus_tag="IW774_000892"
     CDS             complement(9035..9610)
                     /locus_tag="IW774_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1153255.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(9615..10589)
                     /locus_tag="IW774_000893"
     CDS             complement(9615..10589)
                     /locus_tag="IW774_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1153256.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEPICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(10613..10657)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            10872..11840
                     /locus_tag="IW774_000894"
     CDS             10872..11840
                     /locus_tag="IW774_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1153257.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            11937..12230
                     /locus_tag="IW774_000895"
     CDS             11937..12230
                     /locus_tag="IW774_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1153258.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            12348..12584
                     /gene="secG"
                     /locus_tag="IW774_000896"
     CDS             12348..12584
                     /gene="secG"
                     /locus_tag="IW774_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1153259.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            12696..13442
                     /locus_tag="IW774_000897"
     CDS             12696..13442
                     /locus_tag="IW774_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1153260.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            13445..15802
                     /gene="rnr"
                     /locus_tag="IW774_000898"
     CDS             13445..15802
                     /gene="rnr"
                     /locus_tag="IW774_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1153261.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            15824..16288
                     /gene="smpB"
                     /locus_tag="IW774_000899"
     CDS             15824..16288
                     /gene="smpB"
                     /locus_tag="IW774_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1153262.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            16590..17309
                     /gene="atpB"
                     /locus_tag="IW774_000900"
     CDS             16590..17309
                     /gene="atpB"
                     /locus_tag="IW774_000900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1153263.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            17362..17577
                     /gene="atpE"
                     /locus_tag="IW774_000901"
     CDS             17362..17577
                     /gene="atpE"
                     /locus_tag="IW774_000901"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1153264.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            17668..18192
                     /gene="atpF"
                     /locus_tag="IW774_000902"
     CDS             17668..18192
                     /gene="atpF"
                     /locus_tag="IW774_000902"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1153265.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            18179..18721
                     /locus_tag="IW774_000903"
     CDS             18179..18721
                     /locus_tag="IW774_000903"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1153266.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            18744..20300
                     /locus_tag="IW774_000904"
     CDS             18744..20300
                     /locus_tag="IW774_000904"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1153267.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            20314..21216
                     /locus_tag="IW774_000905"
     CDS             20314..21216
                     /locus_tag="IW774_000905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1153268.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            21239..22645
                     /gene="atpD"
                     /locus_tag="IW774_000906"
     CDS             21239..22645
                     /gene="atpD"
                     /locus_tag="IW774_000906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1153269.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            22659..23081
                     /locus_tag="IW774_000907"
     CDS             22659..23081
                     /locus_tag="IW774_000907"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1153270.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         7799 a         4203 c         4950 g         6202 t
ORIGIN      
        1 atctatacag atacaacaaa aaactgtcca ccaattctct ttggcggaca gttttcactt
       61 aaaaataaag aagcaactat tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag
      121 ggaatcgaac ccccgtctca agaaccggaa tcttacgtga tatccactac actaaggacg
      181 caagcactgg gataattgta actaaacaga taaaaaatta caaggggatt ggacaaaaaa
      241 atgaaatttg agcaaaattt ttcacaaaaa cagcagcaga cacagaaact agccatgacg
      301 caacagttgc aacagtccat ccaagtttta caatataaca gtgaggagct gttggcattc
      361 gttgagaatc aagcaatgga aaatccattg gtggaagttg ttgagccgga atggcagcca
      421 gattacataa aagcttcttc atcttcttat gaaggagaag agacaaatta cctaaatcag
      481 attcctgata caaaagggtc tttatttgat tcattgatcg aacaagtcca tttgaattac
      541 cgagatactt ttttgaggaa aatcgttctt tatcttgtag aatacatcga tttgaacgga
      601 tttttgacga ttagtttaga agaagccgga aaagagattg gagctacgcc tatccaaatg
      661 ctcgatgcgt tgactctgat tcagcaatta gaaccagctg gagtaggtgc acgtagtttg
      721 caagaatgtc tcatgcttca aacagaacga gatgattatg cgccggaatt agcttatatc
      781 gtgttggaag aatgttttga ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt
      841 ttttctgttg atctacatgc tgttcagcag atattcgact atttacaaac gttgagtcca
      901 agccctggcc ggattttcga ccgtagcagc gaattgtaca ttattcctga tgtccgggta
      961 ttagtggacg acgataaaaa tgtacaagtt atctcaaacc gaaaaaatca gccaaatata
     1021 cgctttcaag agagttactt caaacaaatg tcacaacaag cagataaaga aactgaaaat
     1081 tatttaaaag aacggaaaca ggaattcgaa tggttgaaga aaacgatcct ccaacgtggg
     1141 gatacaattt tgcgggttgc tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac
     1201 aaggaacgtc caatcaagcc tcttacgtta aaggaagttg caactgagat agatgtgcat
     1261 gaatccacgg tcagtcgtgc agtcaacggg aaatatttgg aaacagattt cggtgtattt
     1321 gaattaaaaa aatttttcac tacacgaatt cctacgaata gtacagaaca gacagaagac
     1381 ctttcagcag atacagcgaa gaaaaagctg caggaattag tcgatcaaga agacaaaaac
     1441 aagccgttgt cggaccagaa attggttgaa ttgctgaaaa aagatgagat agcgatctca
     1501 agacgaacag tagcaaaata tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa
     1561 cgttatgata actaatcagt caaaagacta gaaaaagtat ttcacttttt ctagtctttt
     1621 tagttgaaaa acgagagcaa aactgttaga cttacagtcg tgaggttgag atttcccttt
     1681 attgatgtga aattcgaata gttaatttct ttttttatcg tatatgggtc gttttaagtc
     1741 tacattggac gtaaagtgtc caactaagga gatcgctatg ctggatgaaa taaaaatgat
     1801 tgaatctgtt gcaccagata ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa
     1861 tatctattgg atgcagccga ttgggcgtcg aagtttatcg gaaagtatgg ggatcacaga
     1921 acgagtctta cgaacagaaa cagacctttt gaaaaatctg aatttgatcg acacctcaaa
     1981 aagcggtatg acgctgacaa ctaagggcga agaagtttat caaagccttg aaaacttcat
     2041 ggatcagctt ttaggtacgc atcaaaccga acaacagttg gcagaatatt ttggtaacca
     2101 acgctgtatc gttgtttcag gaaacagcga agagcagacc aaagtggctg atgcattcgg
     2161 cgaagcgctg agcgaagcct tagaccgact acttccagaa ggcgagaata tcatcgctgt
     2221 gatgggagga actactatgg ctgtggtagc cgaacaattg tctaatctgg aaaataagaa
     2281 acggcataac ctgtttgtcc ctgcaagagg cgggattggt gaagcgataa ctgtacaggc
     2341 aaattctgtc agtgcaagaa tggctgctaa agcaaacggt aaccaccgcg cactttatgt
     2401 tccggaacaa ttaagtctgg caacttacaa ttctttactc aatgagccat ctatccaaga
     2461 agtcttaaac ttgattagtg aagcgaattg tgttattcat agtattgggc gtgcattaca
     2521 catggcagca cgtcgtaaga tgactgaaaa agaattagtc atgctgaaac aagcaaatgc
     2581 tgttgcggaa tcttttggct actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc
     2641 tagaatcggc atagaattgc aagatttgga aaaagtcccg atcatcatgg caatcgcggg
     2701 cggaaaatca aaagctaaag caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg
     2761 gctcatcact gatgaagctg ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa
     2821 atattttttt gattttcata aggaggaaat cttaaatgac agttaaagta ggtattaatg
     2881 gttttggacg tattggacgt ctagcattcc gtcgtatcca agatgtagat ggaatcgaag
     2941 tagtagcaat caacgacttg acagatgcta aaatgttggc acacttgtta aaatacgaca
     3001 caactcaagg acgtttcaac ggaacagttg aagttcatga aggttcattc aacgtaaacg
     3061 gaaaagaagt taaagtatta gctaaccgca acccagaaga attaccatgg ggcgaactag
     3121 gcgtagatat cgttctagaa tgtactggtt tcttcacttc taaatcagca gctgaaaaac
     3181 atttaacagc tggtgctaaa cgcgttgtta tctctgctcc tggcggaaac gatgttccaa
     3241 caatcgttta taacacaaac cacgaaacat taactggtaa agaaacagtt atctcaggtg
     3301 cttcatgtac tactaactgt ttagctccta tggctaaaac attgaacgac aaatttggtg
     3361 ttgttgaagg attaatgaca actatccacg cttacacagg tgaccaaatg actctagacg
     3421 gacctcatcc taaaggtgac ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta
     3481 actcaacagg tgctgctaaa gctatcggtt tagtaattcc cgaattgaac ggtaaattag
     3541 acggagctgc tcaacgtgtt cctgtaccaa caggttcatt aacagaatta gtaacagttc
     3601 ttgaaaaaga agtaactgtt gacgaaatca atgcagcaat gaaagaagct tcaaacgaat
     3661 cttacggata caacacagac gaaatcgttt cctcagatat cgttggtatg acttacggtt
     3721 cattatttga tgctacacaa actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa
     3781 ctgttgcttg gtacgacaac gaaatgtcat acactgcaca attagtacgt actttagaat
     3841 acttcgctaa cttataagat agacaaacgt acaagtgtaa attatagaca tgtacattta
     3901 aaagcgggga agcaatcgcg cttcttccgc tttttttgat aataagttta ttcaggagga
     3961 aataaaatgg ctaagaaaac agtgaaagat atcgatttaa aagacaaaaa agttcttgtc
     4021 cgtgttgact tcaacgttcc tttgaaagac ggcgtgatca ctgacgacac tcgtatcaaa
     4081 gcagcattgc caacaatcaa ctacgttcta gaacaaggcg gaaaagcaat ccttttctct
     4141 caccttggac gtgtaaaaac agaagaagat aaagaaggaa aatctttagc tccagttgca
     4201 aaacgtctag gcgaattatt aggtaaagaa gtaacattcg tacctgaaac tcgcggagaa
     4261 caattagaag aagcaatccg caacatgaat gacggcgacg ttgtcgtatt cgaaaataca
     4321 cgttttgaag atattgacgg taaaaaagaa agcaaaaatg atacagaact aggtaaatac
     4381 tgggcttcat taggcgatgt atttgttaac gatgctttcg gtacagctca ccgtgcacat
     4441 gcttctaacg taggtatcgc ttcaactggt atcccaacag ttgctggatt cttgatggaa
     4501 aaagaaatca agttcatcgg tgaagcagta gaagaaccaa aacgcccaat gattgcaatc
     4561 cttggtggcg caaaagtttc tgataaaatc ggcgtaatcg aaaacttgat tcctaaagca
     4621 gataaaatct taatcggtgg cggaatgact tacacattct acgaagcaaa aggaatcaag
     4681 atcggtaact ctctagtaga agctgataaa gtagaactag caaaagaatt aatcgaaaaa
     4741 gcaggcgaca aacttgtctt gccaatcgac aatgtctgtg caccagaatt ttcaaatgat
     4801 gttgaaacac aagtaatcga aggagacatt cctgatggtt tgatggcttt agacatcggc
     4861 ccagcatcag taaaattatt tgctgataca ttacaaggtg caaaaacagt tgtttggaac
     4921 ggacctatgg gtgtatttga aatgagtaac tttgctaaag gtacaatcgg tgtttgtgaa
     4981 gcaatcgcta acttagaagg cgcaacaaca atcatcggtg gcggagactc agctgcagca
     5041 gcagaacaat taggattcgc tgacaaattc acacacatct caacaggtgg tggcgcaagc
     5101 ttggaattgc ttgaaggtaa agaattacca ggactagcag caatcaacga taaataaacg
     5161 accaagtttg ctcaattctg acgagtaatc gatcaaaagg tgaagggagt tttcatgact
     5221 ccctagccat ttttcaataa attaaggagt gtgttcctca atgcgtaaac ctattatcgc
     5281 aggtaactgg aaaatgaaca aaactgcatc agaagcaaaa gaatttgctg aagcagtaaa
     5341 aaacaaaatc ccttcaaatg acaaagttga ttcagtcatc ggatctcctg cattattctt
     5401 acaagaatta atgacatcag ctgaaggtac agaactaaaa atctcagcac aaaactgcta
     5461 ctgggaaaac tcaggtgcgt tcactggtga aacttcacca gcagctcttg ctgatctagg
     5521 tgttcattat gtgatcatcg gacattcaga acgtcgtgaa tatttccatg aaacagacga
     5581 agacatcaat aaaaaagcaa aagctatctt tgcaaatggt atgacaccaa tcctatgctg
     5641 cggcgaatct ttagaaacct atgaagcagg caaaactgct gaatggatcg aaggacaaat
     5701 tacagctggt ttagctgatc tttcagctga acaagtgtca aacatggtta ttgcttatga
     5761 accaatctgg gcaatcggta ctggtaaatc agctgacgca caaatcgctg acgaaatttg
     5821 tggagttgta cgtcaaacag ttgaaaaact ttatggaaaa gaagtatcag aagcagttcg
     5881 tatccaatac ggcggttctg taaaaccaga aaatattgct gaatacatgg caaaagaaaa
     5941 tgttgacggt gctttagtcg gtggcgcaag cttgcaagca gattcattct tgtcattgtt
     6001 agacgctgta aaataattgt tgaaaatttg tgaaaaatgc aagaaatgtt tgcactaatg
     6061 agcaaaatta actaaaatcg tatatgaggg aaatccctta ggaaaaaaca aactcaaagg
     6121 agagacaaaa catgtcaatt attactgatg tttacgctcg cgagatctta gactcacgtg
     6181 gtaacccaac aatcgaagta gaagtataca cagaaagcgg agcttttggc cgcggaatgg
     6241 ttccttcagg tgcttcaact ggtgaatacg aagcagttga attacgtgac ggagacaaag
     6301 ctcgttatgg cggtaaaggt gtaactaaag ctgtcgacaa cgtaaacaac atcattgctg
     6361 aagcaatcat tggctacgat gttcgtgacc aaatggctat cgacaaagct atgatcgctt
     6421 tagacggaac tcctaacaaa ggtaaattag gtgcgaacgc tattttaggt gtttctatcg
     6481 ctgttgcacg tgcagctgct gactacctag aagtaccttt ataccactac ctaggcggat
     6541 tcaacacaaa agtattgcca acaccaatga tgaacatcat caacggcgga tcacatgctg
     6601 ataactcaat cgacttccaa gaattcatga tcatgcctgt tggcgctcca acattcaaag
     6661 aagctctacg tatgggtgct gaagtattcc acgcattagc ttcaatcttg aaagctcgcg
     6721 gattagcaac ttctgttggt gacgaaggtg gatttgctcc aaaccttggt tcaaacgaag
     6781 aaggttttga agtaatcatc gaagcaatcg aaaaagctgg ctatgtacct ggtaaagacg
     6841 ttgttcttgc tatggatgct gcttcttcag aattctacga caaagaaaaa ggtgtttacg
     6901 tactagctga ctcaggcgaa ggcgaaaaaa caactgacga aatgatcaaa ttctacgaag
     6961 aattagtttc taaatatcca atcatctcaa tcgaagatgg attagacgaa aacgactggg
     7021 acggattcaa gaaattaact gacgtattag gcgacaaagt tcaattagtt ggtgacgact
     7081 tgttcgtaac aaacactcaa aaattatctg aaggtatcga aaaaggaatt gctaactcaa
     7141 tccttatcaa agtgaaccaa atcggtacat taacagaaac tttcgaagct atcgaaatgg
     7201 ctaaagaagc tggctacaca gcagttgtat ctcaccgttc tggtgaaaca gaagattcaa
     7261 caatctctga tatcgctgtt gcaacaaacg ctggccaaat caaaactggt tctctatcac
     7321 gtactgaccg tattgctaaa tacaaccaat tactacgtat cgaagaccaa cttggtgaag
     7381 ttgcagaata caaaggtttg aaatctttct acaacttaaa aaacaaataa ttcttttcta
     7441 atgaagatac atgatcgact attgaaagat agtcgagtat gatcacttag ttaagtaatc
     7501 gaactctcta cttgctgaaa tatcagcagg tagagagttt ttttgcaact agcttctgta
     7561 tatgatgttt acaaagaatg gtacaagaaa agatcatcct tgaaaaagga aagctaacaa
     7621 taactagcat catataagag cttttaagga tagaacagta gaataggaat ctatgagcaa
     7681 gtcctctcgt ataaataaaa taggagggaa gtagcagcat atttttcata aagtggctct
     7741 ctttcttcat ttttactttg gttgctccta tttgaaaatt cgagcacact caaatacaat
     7801 aaaaagaaat ggcaagggag aggattagat gaaaaaagaa gaaaaaatcg cgattttaca
     7861 agagatcatc cgaattaaat cagtgaatgg aaacgaaggt gaagtagcag cctatttgaa
     7921 taagcttttg gcaaaacatg acataacagg agagattgtt tcttacagag atggtagaga
     7981 taatttgatt gctcgttacc aaaagggcca atcaggaaaa gtgttaggcc tatctgggca
     8041 tatggatgtc gtggcggctg gtgatgaatc gtcttggacc tatgcaccct ttgcagcaga
     8101 aatccatggg aaccgtttgt acggacgtgg tgctacagat atgaagagtg gcttggcagc
     8161 aatggtgatt gccatgatcg aactgaaaga gtcaggaaaa ccatttaacg gaacagtcaa
     8221 attattagcc actgtcggag aagaagtggg tgaactaggt ggggaacagc tcacgaaagc
     8281 aggctatgta gatgacttag atgccttgat catcggagag ccaaccaatt acagtttgat
     8341 gtatacgcac atgggatcaa tcaactatac agtcacctcg cacggaaaag aagcgcatag
     8401 ctctatgcca gaccaaggtt ataatgcaat caatcaccta aatgaattca ttacgaaagc
     8461 aaatgctgaa atgaatcacc tcgctgaaac aatcgagaat cctgtcctag gaaaaacaat
     8521 ccataatgtt acattgatca gtggcgggaa tcaagtcaac agcatcccaa gccatgcgca
     8581 gctccaaggg aacattcgat cgattccaga atacccaaat gataaaatca ttgcattgct
     8641 gcaatcaatc gtcaatgaat tgaatcaaga gacagactat catttggaat tgatgatcga
     8701 ttacaacaaa atacctgtca aagctgatcc agattctcct ttgattcatt gcattcagca
     8761 acaattcagc cagccattac ctttagttgg agcggcagca acaacggatg cagccgaatt
     8821 tacgaaagct aaccattcct tcgactttgt cgtctttggg cctggtgtcg ttactttgcc
     8881 tcatcaagta gatgagtatg ttgaaatcga taattatttg gatatgatcg aaaaatatca
     8941 aggaattatt ttatcctact tagcgtaaga tttttttaat aaatgaaaaa acagagccag
     9001 attagcatta ttttgctaat ccagctctgt ttattcagcc tatttttctt tttgaatata
     9061 tcaagcgttg tggcaacata gtgatgacta atgaaacaac gacgatagac aacaaacgat
     9121 caaggtagtc tgtacctgcc tgtaccaaga tcacacttgt tgtctgactt aaacctaacc
     9181 catgtaatag ctgaacgatc atactggatc ctgaggaggt gatcccgcca aataactgaa
     9241 cggtaatgat cgaagaaacg atcgttccag gtaccgacaa aatcaaagcg agtaataaca
     9301 gtttcaagct gtttttatct ttgccgagat atcttggcaa aatcaatcca gcaagcaagc
     9361 ctgtgagaat ttggattggc gaataataca gagaaaaaat atctgtagtc actccactta
     9421 aaataccgct ggacaatcct gtcagcattc caggtacagg cccaagcaat gcaccagcaa
     9481 aaatcgttcc gatagaatcg agatagattg gcaaacgaag ccataaagca atcgttcctc
     9541 caatataatt gatcccaata cacaaagcca tcactgtaag tacgcgagtt gccattttgt
     9601 ttttattcat tttgttagcc catctttaac ctttccaaat ctttcataat cgtttcttct
     9661 tgtgcattta aaagaacggt taaaaacttt cggaaaaaaa gacgagtatc tacttttgtc
     9721 ataattttac tgttagcagg tttctgccaa aattcataac gatcaaccaa tgtttgtcca
     9781 cgacttattc cttgagtttc cacagcagtg aagcttttga aaccttcaca gatgggctcc
     9841 tcgataaaat aagcaacagc taacggatca ttgatgacac atcctaaaat tctttcttgt
     9901 ttccagtgga agtcaaaata aaaacgagtg atcgctttta gatactctcc ctcttcagga
     9961 ttcatttgac agcagtattc gaggatagtc ggtgtcaaga cgatttctct tgtcacatct
    10021 aacccaacca tttctatcgt ttgtttgaga ttctcgaata cataagaagc tgcttcagga
    10081 tcacaccagt aattgtattc agcaactggc gaacagttac catggctttt gtaagttcca
    10141 cccatcgaga caaaacgttc catatgtttc ccaatatttg ggttgacttt caaagcagaa
    10201 gcgatattgg tcaaaggacc caaagcaatg actgatgtat ttgtcttttc tttgaaataa
    10261 tcagccaaaa aatcaacggc atggagaggt tctgcctgtt ttctcaagat catcgggaaa
    10321 tttgtttccc ctaaaccatc cataccatga gtgtcttgtg cactgatgaa aggttgcttc
    10381 aaaggttttt ccattccttg ataaacagga atgtctaaac gatcacagcg ttccaggcat
    10441 ttcagcacat tctgcgtacc tatatctgca gggacattcc cacaaactac ggtaatagca
    10501 actacttcga tatcagggga tttgacagct aatgataaag ctaaagtgtc gtcgatccct
    10561 ggatcgcaat caataattac ttttcgcatt atttccaatc tcctttcttt agagatactt
    10621 agttttttat tctgggaagt ttccgaacca gtccaagacg agcgtcttga ttataatgca
    10681 cgaaattagt atactggctt ttattgcgaa tgtaaatgct ctcttacaag taaatctatt
    10741 gaggagaact ttaagtaaat gatacaatga aaaagaaatg gaatttacgt gtgccgaaaa
    10801 taaagaatcc tatggatcgg tacattggat aatagataga ttatagtttg gcagaaagcg
    10861 gcgggtgtac aatggttatt ttttatttca ttcttattct gataattata gggatcagtt
    10921 accattttct ttcttatttt tatgcaaaga aattagtaag agcgggagta cagagaggat
    10981 atgactggta tgaagacact ggtcatcgtt tgatggaccc tgctgcagtc acgtcagtag
    11041 aaaaaaaacg acaaaaatta gaagaagagt gtgaaagctt ttgggagcag ggaacaccca
    11101 aaacaatcaa aagttatgac gggttgaaac tagctggtca gatgttcatc catccaaata
    11161 agcagaataa atgggtcatt tgcgtacatg attaccgaag cacagggaaa agagacatgt
    11221 ctcatatagg taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg
    11281 cgcacggaga aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa
    11341 ttgcctggat ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg
    11401 gatcaatggg agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca
    11461 aaggatttat tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa
    11521 gtaaaatcac tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga
    11581 aatacgccgg ttactcctta aagcaagcat cagctacgag acagcttggc agcaatcatc
    11641 tgcctttatt agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca
    11701 cgattcaaaa tgctacggca ggggataaag cactgctgct tgttccggaa gcagaacact
    11761 tggaagcatc gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac
    11821 aaagaataac gatgaattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat
    11881 tgtttataga tcaggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga
    11941 ggagtgcggc atatggaaat caaagaagaa aaaatcgtct tgctttattc aatgatgaac
    12001 aacaagagat cggagaaatg acatggtcag atgcgggccc agacatcatg atcattgacc
    12061 atacatttgt agatccagca tatcgtggtc aaaagttagc tgaaaagcta gtgtatactg
    12121 gagtagaatt agcaagacga gacgggaaaa agatcattcc tttatgccct tatgcaaaaa
    12181 aagagtttga aaagaaacca gaatatcaag acgtcttacg tcagtcataa gaataacagc
    12241 tgaaagtttt gaactttcag ctgttattcc atttatatag agttgtcacg gacttttatc
    12301 tatgataaag ttaaagagac gatatctttt gggaaggagg ctgcgatatg tataatttaa
    12361 ttttgggaat cgtgatcgtt ttatcgattg ttatggtcat tgcgattatg atgcagccaa
    12421 gcaagcaaaa tagtgcagcg agtgcattta ccggcggtgc ggatcaacta ttcggcaaac
    12481 aaaaagctcg cggctttgaa gctgtcatgc agcgatcaac tgcagttatg ggagctatct
    12541 ggatgatctt attatttgtc ctagcatatt tatcttcgaa gtagttgaat aagcctctcg
    12601 caaaacggcg ggcggctttt tttattgcaa agagaacgct taagaagaag ttatgaaaga
    12661 aactatggta aaatgaggcg aaagaggtga gacgagtgag acaattacca aaaccccttt
    12721 atgcaaaaca tggaaagcga gcggttttgc tgcttcacgc ttactcagga agtccaaacg
    12781 atgttcgaat gctcgcacga tttttggaaa aagcagatta tacagtttat gcgccgcttt
    12841 ttaaaggaca cggaacaatg gagccatatg atatcttgca agaaaaagca gaaagctggt
    12901 gggcagacac taaaaaagcc atccattttt tacagtctga gcagttttct gatattgccg
    12961 tattaggatt atcaatgggc ggcatctttg ctgtacgagc actagaagaa gaatcagtga
    13021 ttggcggcgg ttttttctgt tcgcctttgt ctcctgtaaa aacaaacgtg cctgaaaact
    13081 ttgaaaaata cgtacgtcaa gtgctgaaga cagcaggtaa atcagagaaa gaaatcaatg
    13141 aaaaagcagt tgcttaccgc tcgttagctg aacaacaatt aatggatata caagatcagg
    13201 cagcaatcgt agaaagtagg ttgtcagata tccaacagcc gatattttta gctcaagctg
    13261 ggaaagatga aatgatcgac cctaatggcg tatttgaaac tgcacgcaag ttgagccgtc
    13321 agcgagtgac acttcaatgg tatcctgaaa gtggtcatgt gattacagtt ggtacagcaa
    13381 gacgcgaatt agaaaaagac gtcttggaat ttttagaaaa attaccttgg aatgaggaat
    13441 aaatatgaca aaagaaacga taaaagagaa aattctcttt ttcatggaaa gcagtcgcaa
    13501 acgcagtttt tctatggaag aaatcgctga aggtttaggg ttccaaaaaa gcgatgattt
    13561 taaattacta gtacaaacga ttgctcaaat ggaaagagaa cagagtatag ttttcaataa
    13621 aaaaggaaaa gtgaaacttc ctttaaaacc aatcttgatc gaaggaacat ttcgagcaaa
    13681 tgaacgagga tttggatttg taacgattga tcccgaagaa gatgacatct atatcccaaa
    13741 agaagcgact ggttttgcaa tggatggaga caccgtagca atcgatatca tgaagcgagc
    13801 aaatacagat atggatcgcg gagcagaggg gaaagtagtc gagattcgag accgggcgac
    13861 tactcagtta gcaggggaat tcgttgctta ttcagaagaa gaaatacaag aaacagatct
    13921 ttacggcgtt gttatcccga aagatagaaa gctcaaccaa tttaaagtat atgcagcagc
    13981 agagggagtt cgtccagttg acggaagtat tgtgctgatc gaactgaccc attaccctga
    14041 aaaagggtat gcgactagtt tagaagggat aatcaagcaa gtgattggtc ataagaatga
    14101 ccctggcatg gatatcctct caattgtcgt ggctaatggt atccctacga aatttcctga
    14161 tgatgtattg tctgaagcag atgaagttcc agatcaaatc agcgaaaagg atataactgg
    14221 acgtagagat ttaagaaatc agctcattgt tacgattgat ggagaagatg caaaagatct
    14281 agatgatgca gttacggtta aaaaattaga taacggtaat tattttcttg gagtacacat
    14341 tgcagatgtt tcttactatg tgacacaagg cagtcaattg gacagagaag catatgaaag
    14401 agggacaagt gtatatttga ctgatcgagt aattccgatg atcccgcaac gtttatcgaa
    14461 tggtatctgt tcactgaatc cccatgttcc aaggctgacc atgagttgcg aaatggagat
    14521 tgatccaaat ggacaagtag tctctcatga tattttccca agtgtgatcc aaacaacaga
    14581 acggatgacc tacacagctg tcaatcaaat cttagaagat caagacgaag aaacgatgca
    14641 acgctatgct cagctcgtac ctatgttcca agaaatgggt gaattgcacc gaatccttga
    14701 aacgatgcga atcaaacgtg gcgcgatttc ttttgaggat cgcgaagcga aaatccttgt
    14761 tgatgctgaa ggacacccaa atgacatcca gctaagaagc agaggaattg gtgaacgctt
    14821 gatcgaatct ttcatgcttg cggccaatga aacggtagct gaacattata ataaattaaa
    14881 tctgccgttt atttaccgaa tacatgaaca gccaaaagaa gaaaaaatgc agcgcttctt
    14941 tgattttgct tcagctttgg gcattctggt ccgaggaacg aaaggaacga tcacaccaaa
    15001 agatttacag catgtgatcg aaggagtaga agacaaacca gaagctgcag ttatcaatac
    15061 tatgttgcta agaagcatgc aacaagcacg ctattcagaa gacaattttg ggcattatgg
    15121 cttagcggca gattactaca cacactttac atctcctatt cgacgctatc ccgatctaat
    15181 cgtccatcgt ctgatccgtt cttacagtga agataaaagt gaaagcaatc aaagttcgtg
    15241 ggctgcggaa cttcctgaaa ttgcagatca tagttctaag atggaacgtc gtgcagtaga
    15301 aacagaacgt gaagtagatt ccatgaagaa agcagaattc atgatggaca aagtgggaga
    15361 agaattcgat gggatcatca gctctgttgt gaaattcggc ctgtttatcg aactgccgaa
    15421 tacagtagag ggattgatcc atatcaatga attgaaacag gactacttcc attttgttga
    15481 aaatcattta gctcttgtag gtgaaagaac tggtctgact tttaaaatcg gacagaaagt
    15541 gcgagtaaaa gtagtcaaag ctgatccaga tgaacgagca gtagattttg aacttgtttc
    15601 cgcagaagag gttacagcgt tggaacggcc aaaaacacga aatggaaaat caggaaaacg
    15661 cgaaggaaaa caagcaggaa aacgcccaga aagaaatggc aagaagaaca aacgatatag
    15721 cgatagtaag caaacgttcg gtaagaaaaa agggaagaag ccattttaca aaggtgttaa
    15781 aaaaaagaaa aagaaaaaat aaaatgagtt ggaggaaaca gctatgccaa aaggcgaggg
    15841 aaaattaatt gcacaaaaca agaaagctcg ccatgattat tcgatcatcg acacgatgga
    15901 agcagggatg gtcttgcaag gaaccgagat caagtcgata cgaaacagcc ggatcaatct
    15961 aaaagatgga tttattcgcg tccgcaacgg agaagctttc ttgcataatg ttcatatcag
    16021 tccttatgaa caaggaaata tttttaatca tgatccgttg cgcacgagaa agttattatt
    16081 acacaaaaaa caaatcatcc ggcttgaaaa tgaattgaaa aatactggaa tcactgttgt
    16141 tcctttaaaa gtctatattc gtaacggcta tgccaaggta ttgattggtc tggcgaaagg
    16201 gaaaaaatct tatgataaac gggaagattt gaaacgaaaa gatatcgatc gacaaattga
    16261 tcgaacatta aaaaatttct ctagataatt tcattatcct ttttcgcata ttcggtgctt
    16321 ttgcttttac ttaatgataa aatttgttag catttaatct gataaaacat gattatcata
    16381 ggttttttct tagatcagga gcaccgtgta taaagaaaaa ttcgcttcgt ttttgactga
    16441 aataaaaaag tgtctaattt tcatgaaaat gttttgaatt ttctgtttca tggtggtatg
    16501 ataccgtgtg gttagaaacc gattggaaaa aaccagatac gcacatgaat tgattttatg
    16561 tgaatttcgg ggaaaagagg tgaaatggat tggatgaacg ctcgctaacg ttccacattg
    16621 gaccaatttg gtttgatggt acagtttgta taatggtgtt actgacctgt ttgattgttt
    16681 tcttcttagc ttactttttt acaagaaatc ttaagttgaa accaacaggc aaacaaaatg
    16741 cgatagaatg ggtcattgat tttagtcgag gaatcgtaac agataatctg cctagaaaag
    16801 aattgagcaa tttccatttg ttggcattta cattgttttt atttgttttt gttgcaaaca
    16861 acatcggttt agtaacaaaa atcgttttac ctaacgaaac aacactttgg aaaagcccga
    16921 cagcagaccc gtttgttact ttaactttgg catttataat gatcttactt actcacttat
    16981 ttggggtgaa aaagttaggc ttcaaaggat atttcgtaaa ttcgttcttg aaaccatata
    17041 gttttatgtt tccgatgaaa ctgattgaag aatttacaaa cttattaaca cttgctttac
    17101 gtctttatgg gaatatttat gcgggggaag ttttgctaac cttgattgca aaaatggtaa
    17161 taagcttagg ctggttctca ttgccgttag caattccatt agagatggtc tggatcgcat
    17221 tctccttgtt tatcggaagc atccaagcat ttgtcttcgt tactttatct atggtttata
    17281 tgagccataa gattgaagtg gaagaataat attcgtttta aaaacaaact actatttatc
    17341 taatttagga ggaaaacaat tatgaattat atcgcagcag caatcgcaat catgggagca
    17401 gcaatcggag ccggttatgg taacggtcaa gttatctcta aaacaatcga atcaatggct
    17461 cgccaacctg aaatgtctgg tcaattccgt acaacaatgt ttatcggggt agctctagtc
    17521 gaagctgttc ctatcttagg ggtagttgtc gccttgattc tagtttttgg tgtttaattg
    17581 aatcagtatt aaggcagaaa caatttggtt tgttactgct tttttacatg aaaactagaa
    17641 gtacttcaaa gaaaggaagg ctcgcctatg ctgaatcaat tggcaattgc agaagttggc
    17701 aatgcgatgt taggaaatgt catcgttgtc agcggctcat ttttgatact actggctctc
    17761 ttgaagcatt ttgcttgggg accaatcagc gatattttga aaaaacgtga agacaagatc
    17821 gccaatgatt tggattctgc agaacaatct cgcatcaact cagcgaaaat ggaacaagaa
    17881 cgcgaacaac aattgttagc ctctcgttct gatgcagctg atatcatcaa aaatgcgaaa
    17941 gaaagtggag aattaagccg ccaaaatatt ttgaaggatg ctcaagaaga agcagctcgt
    18001 ctaaaaagta aagcccaagc tgatatcact gtagaacgtg attcagcgct gaactctgta
    18061 aaagacgatg ttgcagaact atctcttcaa atcgcggaaa aaatcttgaa caaagaatta
    18121 tctccagaaa tgcacgaatc attaatcaat caatatatcg aaggtctagg ttcttcaaat
    18181 gaaactagat aaatatacag taggaagacg ttacgggaaa gctttatttg aactggcgat
    18241 cgattcaaat caagcagaag aaatctatca agatctcgta agcttacgtc aaatctatga
    18301 acaagtacct ggattaggta atatgttaag tgatgttcgt ctagaacctc atgaaaaacg
    18361 agaaatcatg gacaagcttg ttagtggcta tgatggaatc gttaaaaact ttttagaagt
    18421 tgtttatgcg tataaccgaa tggatgatct tttgttcatg atcgaagaat atgaacgtcg
    18481 atatgatgaa catcaaggtt tgctgttggg aagtgtcaca acagcagttc cactttcaga
    18541 cgaacaacga atacaattag aaaagaatgt tgcaaaaaca atgaactatc aatcagtcga
    18601 attaaaacaa atcgttgatt cttctattat tggtggcgcg attgttgaag ctaaccatcg
    18661 agtaatcgac ggaagcattc gcacgcaatt agaaaatatg cgtaatcaat taaatagata
    18721 gtacaaagag gtaggtgaat cgaatggcca tcaaagcaga agaaattagt gccttgatta
    18781 aagaacaaat tgaaaattat caaaacgtgc tttcagtcga agaaatcggt accgtaactt
    18841 atgtaggtga cgggatcgct cgtgcacatg gtttagaaaa cgcgatgagt ggtgagttgc
    18901 ttgaattttc aaatggctca tacggaatgg cgcaaaactt agaatcaaat gatgtaggta
    18961 tcattatcct tggcgatttc gagtccattc gtgaaggaga taaagtaaaa cgaacaggaa
    19021 agatcatgga agttccagtt ggagaggcct tgatcggacg ggtagttaat ccgctaggtc
    19081 agccaatcga cggactaggt gaaatcgtta ctgacaaagc tcgtccagta gaagcaatgg
    19141 caccaggcgt tatgcaacgt aaatctgtta acgaaccaat gcaaacaggt ctaaaagcaa
    19201 tcgacgctct tgtaccaatc ggacgtggcc aacgtgaatt agttatcggt gaccgtaaaa
    19261 cagggaaaac ttctattgcg atcgatacga tcatcaacca aaaaggtcaa gatatgatct
    19321 gtatctatgt agcaatcgga caaaaagatt ctacagttcg tacacaagtt gaaacattga
    19381 aaaaatatgg cgcaatggat tacacaatcg ttgtgaatgc cggtgcgtct caaccagcgc
    19441 cgttgcttta tatcgcacca tatgctggta ctgcaatggg tgaagagttc atgtacaacg
    19501 gtaaacatgt cttgatcatt tttgatgatc tttcaaaaca agctgttgct tatcgtgaac
    19561 tttccttgct acttcgccgt ccaccaggtc gtgaagctta tccaggggat gttttctact
    19621 tacactcacg cttattggaa cgtgcagcaa aattaagtga tgatttaggt ggcggttcaa
    19681 tgactgcctt accatttgtt gaaacacaag ctggagatat ctctgcttat atcccaacaa
    19741 acgttatttc gatcactgat ggacaaatct tcttagaaag cgacttgttc tacgcaggta
    19801 ctcgtccagc ggttgatgcc ggactatctg tatctcgtgt agggggttct gcgcaaatca
    19861 aagcaatgaa aaaagttgct gggactttac gtctagactt agcaagttac cgtgaattag
    19921 aagcatttac acaattcggt tctgacttgg atgcagcaac acaagcaaaa ctaaatcgtg
    19981 gacgccgtac agtagaaatc ttgaaacaaa aattgcatgc accgctagct gttgaaaaac
    20041 aagtagtgat tttgtatgct ttgacacatg gattcttaga tagtatccct gtagacagta
    20101 ttttagactt cgaacatgaa ttattcgaat atctggatac caatcattca gatatctttg
    20161 aaacaatccg tacaacaaaa gatcttccag aagaagaagc gttgaacagt gcgattcagg
    20221 aatataaaga catgttctta gctacaaaag gaaataattc ctctacagaa gataaattga
    20281 aatccattca aaatgcatag tgaggtgaga tgaatgggtg cttcattaaa tgaaatcaag
    20341 acacgtatcg cttcaacgaa aaaaacgagc caaatcacgc gggcaatgca gatggtgtct
    20401 gcttctaagt tgacaaaatc tgaagcttct tcacagaagt ttcaggttta tgcaacaaaa
    20461 gtaagagaaa tcgttacgca tttgactgct actcagttaa atgatattgc ctcagaaaat
    20521 cctagagggg atatcaatta caacagcatg ctgctcagcc gtcctgttaa gaaaactggt
    20581 tatatcgtca tcactgcaga tggcggttta gtcggtggat ataacagctc gatattgaaa
    20641 caaacgatgt ctattttaga agaagaccac caatcacccg atgattacgt gatgatcgca
    20701 attggcggaa ctggtgcaga cttctttaaa gcacgcggta tcaatctagc atatgagctg
    20761 cgcaatctat ctgatcagcc aagctttgat gaagtccgca aaatcgtaaa tatggcaaca
    20821 actatgtacc aaaacgaagt attcgatgaa ttatacgtat gttataacca tcatatcaat
    20881 tcgctaacta gtcagtttcg tgtagaaaaa atgctgccaa tctcagactt agatccagaa
    20941 gaagcaacta ctttcgaaca agaatatatt tttgaacctt caaaagaaga aatcttgtct
    21001 cagttattgc ctcagtatgc tgaaagtttg atctacggtg cgatcgttga cgccaaaaca
    21061 gcagaacatg ctgctggtat gacagcaatg aaaacagcaa ctgataatgc tgctaatatc
    21121 attgatgagc tgacggtttc ttataaccgt gcaagacaag gggctattac ccaagaaatt
    21181 accgaaattg ttgccggagc ttcggcacta gaatagtatt gagattggag gaaaaaagat
    21241 gagttcaggc aagattgttc aagtaatcgg tcccgttgtc gacgtggaat tttctttaga
    21301 ccaatcctta ccagatatca acaacgcatt agttgtttat aaaaatgacg aaaataaatc
    21361 aaaagttgtt cttgaagcag cgttagaatt aggggacgga gtgatccgga ccattgcgat
    21421 ggaatcaaca gatggtttac aaagaggaat ggaagtcatt gatacaggca aagcaatctc
    21481 tgttcctgta ggtaaagaaa cattaggtcg cgtattcaac gtactaggag atacgatcga
    21541 tttagaaaca cctttcccag aagatgcgga aagaagcgaa attcataaaa aagcaccagc
    21601 ctttgacgaa ttaagtacaa gtacagaaat tttggaaaca gggatcaaag ttatcgattt
    21661 gcttgcccca tatttaaaag gtgggaaagt cggactattc ggtggtgccg gtgttggtaa
    21721 aaccgtactg atccaagaac tgatccataa tatcgcccaa gaacatggtg gtatttctgt
    21781 atttactggt gtaggtgaac gtactcgtga aggtaatgac ttgtattatg aaatgaaaga
    21841 ttcaggagtt atcgaaaaaa cagccatggt gttcggacaa atgaacgaac caccaggtgc
    21901 acgtatgcgt gttgctttga ctggtttgac gattgcggaa tatttccgtg atgtagaagg
    21961 tcaagatgta ctgttgttta tcgacaacat tttccgtttc actcaagctg gatctgaagt
    22021 atcagccttg ttaggacgga tgccttctgc ggttggttat caaccaacat tggcaacaga
    22081 aatgggtcaa ttgcaagaac gtatcacatc tacgaaaaaa ggttcgatca catcgatcca
    22141 agcaatctat gtgccagccg atgactacac tgacccggcg ccggcaacag cattcgccca
    22201 tttggatgcg acaaccaact tggaacgtaa attaacagaa caagggattt atccagcggt
    22261 agatccattg gcttcttctt caagtgcttt agcacctgaa atcgtgggag aagaacatta
    22321 taaagtagca acagaagtcc aacacgtatt gcaacgatat cgtgaattac aagatatcat
    22381 tgctatctta ggtatggatg aattatccga tcaagaaaaa gtgttagttt ctcgtgcccg
    22441 cagagtccaa ttcttcttgt cacaaaactt taacgtggct gaacaattta ctggtcttcc
    22501 aggttcatat gtccctgtag aagaaacagt gaaaggattc cgcgaaatct tggaaggcaa
    22561 atacgatgat cttcctgaag aagctttccg aagtgttggc cgaatcgaag acgtcgtaga
    22621 aaaagcgaaa acactaggct actagaaagg ggtgccccat ggatcaatat ttaacagtaa
    22681 atgtggtaac tcctgccggt ttggtctatg atcaccatgc agctatcgtc gttgcacgta
    22741 cgacagctgg tgagcttggg attttgccta aacatgcacc aatcatcgtt ccattgacga
    22801 ttgatgaagt tcgtgtaaaa cgaacagatt cagataccca cgtcgattgg atcgctgtta
    22861 atggcggaat catggaagtt cgtgataatg ttgtttcaat cgttgctgat agtgcggaac
    22921 gtgaacgtga tatcgatgta tctcgtgcgg aacgtgcaaa acaacgcgca gaacgccaaa
    22981 tcgaagaagc aaaagcaaaa caagatacga acgaattgaa acgggcaact gttgcgttgc
    23041 accgagcaat caatcgtatc aaagtatcta agcactcata aaatgagatg cggatgttta
    23101 tccgtgttta agaacagtaa aaagaggtta tgacagaagt ggtcagcttc aaga
//
LOCUS       DACEOE010000027        22640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEOE010000027 DACEOE010000000
VERSION     DACEOE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.028"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1182..1847)
                     /locus_tag="IW774_000908"
     CDS             complement(1182..1847)
                     /locus_tag="IW774_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1153271.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1973..4009
                     /locus_tag="IW774_000909"
     CDS             1973..4009
                     /locus_tag="IW774_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1153272.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4013..4651
                     /locus_tag="IW774_000910"
     CDS             4013..4651
                     /locus_tag="IW774_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153273.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4679..5107
                     /locus_tag="IW774_000911"
     CDS             4679..5107
                     /locus_tag="IW774_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153274.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5564..6475
                     /locus_tag="IW774_000912"
     CDS             5564..6475
                     /locus_tag="IW774_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1153275.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6692..7129
                     /locus_tag="IW774_000913"
     CDS             6692..7129
                     /locus_tag="IW774_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153276.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7224..8108
                     /locus_tag="IW774_000914"
     CDS             7224..8108
                     /locus_tag="IW774_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1153277.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8332..11499
                     /locus_tag="IW774_000915"
     CDS             8332..11499
                     /locus_tag="IW774_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153278.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11519..12064
                     /locus_tag="IW774_000916"
     CDS             11519..12064
                     /locus_tag="IW774_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1153279.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12092..12547
                     /locus_tag="IW774_000917"
     CDS             12092..12547
                     /locus_tag="IW774_000917"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ1153280.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12544..13302
                     /locus_tag="IW774_000918"
     CDS             12544..13302
                     /locus_tag="IW774_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1153281.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13388..14128)
                     /locus_tag="IW774_000919"
     CDS             complement(13388..14128)
                     /locus_tag="IW774_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1153282.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14189..15442
                     /locus_tag="IW774_000920"
     CDS             14189..15442
                     /locus_tag="IW774_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1153283.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15764..15991
                     /locus_tag="IW774_000921"
     CDS             15764..15991
                     /locus_tag="IW774_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1153284.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16114..16440
                     /locus_tag="IW774_000922"
     CDS             16114..16440
                     /locus_tag="IW774_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1153285.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16508..17221
                     /locus_tag="IW774_000923"
     CDS             16508..17221
                     /locus_tag="IW774_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1153286.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17531..18382
                     /locus_tag="IW774_000924"
     CDS             17531..18382
                     /locus_tag="IW774_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1153287.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18503..20368
                     /locus_tag="IW774_000925"
     CDS             18503..20368
                     /locus_tag="IW774_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1153288.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20416..21825
                     /locus_tag="IW774_000926"
     CDS             20416..21825
                     /locus_tag="IW774_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1153289.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            21934..22635
                     /locus_tag="IW774_000927"
     CDS             21934..22635
                     /locus_tag="IW774_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1153290.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
BASE COUNT         7630 a         3690 c         4657 g         6663 t
ORIGIN      
        1 ctaatatatt catatgagct aattttatga ttaaatggta agtgtttcct tgtttgtttt
       61 agtttaaact agtgtgtaat ttaaaaatct atatggaagg gtctgtgggg taatacttaa
      121 ataaaggcta ggaacctgat aacatcaagg ttcctagcct ttttataata aaatgaagcg
      181 aaaataattt cttttaaaga gccgagatta tcagctatat aagagtgaat ttgatgcaaa
      241 aaacagagcc gaaacagtct tggtgttcgt agctctgctt atatgtttcg aatttaagac
      301 aaagagttgg caaaagatgt tgataaggaa gataagataa ctgtgttata taaactaggt
      361 aactaactgt aacaagctaa ttttaagaga ttcttattag aagacgttta ggaatataac
      421 taagaaaatg attatacagg taattgtcat ttcaataatg ttttctaatt ttcgcgtctt
      481 tgttttaaga aaaaggccag aatcagttgg aaaaaagttc gtacgatagt gaagaggggc
      541 aaaatttcct actaatattc ccactaacaa gagataaaac ggccaggttg gtgtgattcc
      601 tctataaaaa aatgcccatg ctaccgaaat cccatagatt aacaactgaa caatttttac
      661 aatgaaaaaa ttgttctgcc attactcaaa gtctcttcct ccttgtatcg tttatttgtt
      721 tcattttatc attatgatag gcgcttgaac aatccatttt gtattttatt gagtactctt
      781 ggataaacta ccagcgatta tcatatttat ttatgttact gtaagccatc atataaaagt
      841 aaatgagcag atcatagaaa tataaaaaag aactaaaatt gatctaaatt atgtgcagct
      901 tgactacgta ccaatctata ttacttacta gattggtcgt taatctattc ggatcgaatg
      961 agcaaaatat ttttgtgaat gtccatcctt gattatgatt ccaaaaagaa tcattgggag
     1021 gctgtggcgt aaatattata ctataagcca gaaataccta tcattctgaa cttttttatt
     1081 taggaacttt catctcaagg ctttttcaat taatttgtac ccctgttttt tggataagat
     1141 catgtgcatt gattttttct atctctacgt tcatctccta cttaatgcct atctggctcc
     1201 tgagggcttc tttttaaggc gtcgtcatag tcttggctga ggatttcttg gtaatctgag
     1261 ggaatggctt cccaagtgag caggcgaacg ttcatgacgt gttttccttt gtgtttgatt
     1321 acagcatggt ttttctcttt ttctacctct ccagcgtgaa aagacagcca tgtaccatgg
     1381 ccaaagatat cattacatac ccaacgttcg atggaattgt ccacgtatcc tacagggagt
     1441 gtggtatagc cgatttgtgg tttcaaaaaa ggattgaatc gatcgatcgt ttcatttttg
     1501 tattgataat tgataagaaa aagatagacc aaacaaaaga cgagcagaaa gaaagatagt
     1561 gtctttttga gtgagaaggg ttgttttgat gaataagatt tcttgttaac aagaggttca
     1621 tctggcaatg ttggttctat tgattcggta gctgattcaa tagaaggaaa atcaatcggc
     1681 agacctaaag tatgatgtaa aagtgacagc atttcaaggt ctggcattgt acgagaattt
     1741 tcccaactag aaatcgtttt atcggaaaca ccaatttttt cgcctaattc tttttgcgta
     1801 agcttacaat ttctgcggta agttcgtatt ttttctccgg tgttcatctt cttctccttt
     1861 cgattctaca gtatatagat ttattctaca tgacctatac acttgataga tacgtttttt
     1921 tgatgaaaaa cggtatgatt ttatcaaaga atcatttaag taagggagag tggtgataat
     1981 caaaaaaaag ttcacagttt tactcacaat catagtgacc ggtatcatga gtggttttgc
     2041 cttggttagt ataaataaac aggtactcta tcggacaaat gaatttttcc atcaaacaga
     2101 tacttatact ccgattcgat tgccacaaca ttttattggg caagaagaat atgagcagct
     2161 aatcatggta tttgaaaatg tctcatcaga aactgagctt tcttatatga aaaaaacagt
     2221 aaatgaatcc aatgaagtag atacatcttg gaacttcaag atacagccaa agcttgaaat
     2281 gacattgtat tatcaagggg atgtcaaatc ttctcacgag aaggtaccct attctaatac
     2341 ctcctttcgc gtaacgtcca ttgaagaatc gatcaaaata aaagattttg aaggtgaatt
     2401 tttcttgatg acgagagaaa aagaagccta tgaacggttc atttcactat tcactgatcg
     2461 attcaatcaa gcatttcaca ctgattatgc cactaatatt tttgaagact ttgaaaatag
     2521 ctattcgtat tcggttattt caaatacaga cgtatataat tatcgagtgt atttaactat
     2581 tggattgatt cttttattcc ttttgttatc atcctttttc ttattgatga ataaagaaat
     2641 cagtttattg ttgaataatg acttttcatt acatcaagta ttgtggtgtt tattggggaa
     2701 aaagaaaatc attattacat tcataattgc aggaatcatt ggtggtttta tttttatgta
     2761 ttataaggag caatttcttt atttgttcca gatgtacgtt ttcttgttca gcggaatata
     2821 tggtatctct ttgatttctc ttttattagt tgaaaaaatc gcttcgttaa gagaagcgtt
     2881 caactatatc caatacattc tttttacatt tggattgttg ttttctcttc ttttgaccgt
     2941 tgccaatgtc gaattagcaa ctatcgtttt ttcttcttca cagttagctg tttcccatga
     3001 accaccaaaa gaaatcgctg gtgaggacta tcatgtattt tatccagtaa ccatcgggaa
     3061 aaaccacgtg gattttattt attccaatcg ttttgcatca gcagcagatc aggaattata
     3121 tgaaactttg aataaaaacg gaagtatatt agtcaatgta tcagattact tgaatgaaga
     3181 aaatgaacag tttggcagag ggataaaaat aaatctgaat tatcttaaga aatttccttt
     3241 gattgatgtc tctggaaggt tgatccaaat aaccgaaaaa gaaacgcacc ctgttatatt
     3301 gatccctgaa cgatatagac atacagattt aaaaaatgac ctggcgcaaa taacagaata
     3361 ttatcaagaa atcagtgaaa aagaaccgaa atttctattt ataaaaaaca atcaacccat
     3421 ttatacattt attccttcta tcccctggat cgaacattat ccggttgttg aaatactgac
     3481 attgaaaaac agtatgtatt tggaaagaaa tattctcagc ggagaaatct atccaccgtt
     3541 aaagatcaag ataggctctt tgtcgaaaga aaggttattt gagctgattg aagaaagtca
     3601 gttacgtgat aacttgcagt tatcatttcc gtatactcag actgaagaga tagctgtcaa
     3661 agtgttaagc agatcatttc attacttgat acagatattt ttactcactt tcttttcttt
     3721 cttcttgttg agctatgtca tgttatgcat atattttgtc tacaattgtc ggaaaatcgc
     3781 tatctttcgt tcaagtggtt attcgctctt tgaaacgtat aaagatttct tcatgatgaa
     3841 cctcatcaaa tggggaacga caagtgtaat cttccttttt cttatagaaa gagagcctaa
     3901 atatcttttt aatatttttt tctttagctt cattgatttt attctttcag ttgtttttat
     3961 tttgtctaca gaaaagaaaa gtcagttatt gctgatgaat ggaggttaac aattggagcc
     4021 gttgatagtg ataaaaggtg cgaaaaaaat cattcgaaac aaaacgattc ttcatttgga
     4081 tcgtttcact gcggatgctg gggatcttgt gactgttgtt ggagaaagtg gagcaggtaa
     4141 aagtacgtta ttgaatatat taggattgaa tgatcagttc agttcaggac aattctattt
     4201 gttcgatcaa gagacgaaac aattaactgg gagagagaag cgcttgataa aaagaaatac
     4261 catatctttt ctttttcaag attttgggtt gattgaatat gaaacaatcg attttaattt
     4321 agatattgga ttgaagtttt cgaatttaaa tgtttcagat aagcgaacag caaagtatga
     4381 ggcattgaaa aaagtaaata taaacaagaa gctaagtacg cctatctaca cattatctgg
     4441 tggtgagaag caaagaatcg cctgtgcgag agtcttattg aagaatagca aaatcatttt
     4501 agccgatgaa ccgactgggt cattggataa aagaaataag gataaagtag ttgaaatctt
     4561 atcgcaacaa gcttcccaag gtacgttagt tatcgtagtc acgcatgatc catatctgac
     4621 caaaatagga aacaagaaga tcatgcttta aaaatcaaaa aggaaaggat gtcgttttat
     4681 gaaaaaagca gttattattt gtgcactagg gataggtttt gttggaggaa acgctatgtc
     4741 atctgaagtg gaagcaaatt cagttcacgg tgaaatctat tacaaatctt taaagccagg
     4801 agagattcat tttgatgcta aaggtaatat tattaagtca aatttaccag tagaccggga
     4861 aatttctaga acagcaatta atcatagctc tggaagatgg gtttattata gtgatacaac
     4921 ttgtacgaga aaaacaggtc attctaacca ctatagtcgt gtctataatc gtcacggtgc
     4981 aaaagcgaaa gttggaaact ctattcgatc agcaaatgca acaaaaggag tatgggccta
     5041 tgcaagtgcg acaggaaata gaactgacac attctcatgt tggtacaatc caaccggttg
     5101 gtattaagta tccaaaagtt tatataaaat agaaaaaaca tataatgaaa cagcgttaaa
     5161 agttattttt ggtagtttta atatattcat ttttagttgt ataaaaatat atcataataa
     5221 aaacaacaaa aaaatgtctg gaaaatcgaa cataaaagtg tctgatttga atatcccagc
     5281 agcggaagaa gaaaaacaaa atcgataaca tttgttctaa gaattagtta cgttaaatac
     5341 ggaacgcaag atagacttga aaaacggtaa caaaagaagt agtatattaa ggtgtgatgt
     5401 gctacttgac atttattctg ctgagcgaac tttctggatt gtgtagcctt atatctattc
     5461 aggacgtatc gagcagatta gtaaaggtgt tcttgatgat tttcatcttg aacacctttg
     5521 tttttagcaa ttcgcaaaaa acgaaaagta ggagaattga taaatgaaaa aaagattttt
     5581 agcacttgcc atcgtactag gcactggctt attgtcaggc tgtacgaatg ccggtgaaaa
     5641 aacagccgtt agttataaag gcggtacaat cagcgaacaa gaagtaatgg atagcttgaa
     5701 aaaaatgcaa ggagcagatt ccgctgtgca acagttgatc gtttatcaag tatttgaaga
     5761 taaatatgga gatgacgtat caaccaaaga aatcgattct caatatgatc aaacaaagaa
     5821 acaattgggt gattcatttg attcacaatt gaaatctgca ggttacacag aacaaacatt
     5881 caaagacagt atcaaacaaa gtctagcctt tcaagaaggt ctaaagaaac atatcaaatt
     5941 gaccgatgaa gatttaaaaa cggcttggga atctttccat ccagaagtgg aagctcaaat
     6001 tatccaagta gcaagtgaag atgatgcaaa agatgtgaag aaagctgctg ataagggaga
     6061 cgatttctct aaattagcca aagacaagtc aactgatact acgacaaaag aagatggcgg
     6121 aaaagtgaaa tttgattcca ctactacaac ggttccggca gaagtcaaag aagcagcctt
     6181 taagttgaaa gatggtcaag tttctgatgt gatcacttct accaatgctt caacttatac
     6241 tacggaatat tatgtggtta aaatggtgaa aaaccaaaac aaaggcaacg acatggataa
     6301 atacaaaaaa gaattgaaag aaattgcaac agacacaaaa ttaagtgaca gtaccttcca
     6361 gaataaagta attggcgaag tattgaaaga cgctaatgta aaaatcaaag acaaagactt
     6421 tgaaaatgtt ttgagtacat tcacttcaga ttctagtaca gcaagtagta aataagcggt
     6481 ataaataaaa aatatgaact atttaaatca ataaaactgt tcatctaaat ctttttatta
     6541 gaactaaatc aacagtagtt taattactac ttgatttagt tctttttatt ttaaaaattt
     6601 tttttactta gaatccagcg accctagata tttttctgta taatcttaaa taaagaaaac
     6661 aatatcaaaa aatgcacgta gaagtagata aatggagtgg ttttgcaaat ttcttttatt
     6721 tctagaagtt attcacgtat acggatttgt ttttctcaca aataaaaagg caggaagtac
     6781 aatggatcat ttgtatataa aatatattca tcaattttcc gttttggtgt taggcacact
     6841 aattatagcg gggattgtcg catttattat tcaaggccgt gacatattaa cagtacatca
     6901 agagtttttc agttatgcat ttggtattcc tctgatcgct ataaggtctg ataacaaaaa
     6961 gtggaagaaa cgctattaca ttctcttgct atttttgtgt ttttatctgc cacctaagat
     7021 ttttacttta ttaggtatac aatgcctatc attttctttt atttcctata tcattgtttt
     7081 aattttcctc agtttcatag tatggatgtt ttatcttgtc aaaaaataaa tgaatgtttc
     7141 atgaacagta cttcatcaca gcctaatttt tttctttatg ctatcgttat attaaagaaa
     7201 tcaatcagaa gggaaagaaa gtgatgaaaa aagttttatt agtggtaaac cccagttcag
     7261 gcggtgaaca agcaaaggaa tttgagcaat tagcgatagc aaaattggaa tcggtgtttg
     7321 atgaagtggt cgtcttgcat acaaaaaaag caggggatgc aaaaaacttt actcgcgaag
     7381 cggctacgga agggtatcat agtgtgtttg tcatgggcgg agatggaaca gtcaacgaag
     7441 gaatcagtgg aatcgctgaa caagaacatc ggccaaattt cggatttttc ccattgggta
     7501 cggtgaatga cttggctcga gcattaggga taccgctaga acctgaggaa gcgatcaatc
     7561 atttttcgat tgaatcagtc aagccattgg atattggaaa aataaatgac gactatttta
     7621 tgaatgtcgt cgcaattggc tctatccctg aatcaatcaa tgatgtagat gcggagaaga
     7681 agacgaaatt tggaaaacta gcttatttca tgtcaggaat caaacagtta gcttccacac
     7741 aatcctactc gtttcacgta gaagttgatg gaaagaagga agaaattgag agcagtacct
     7801 tgctgatagg actaactaac tcagtcggcg gttttgaaac attacttcca aatgcaaaag
     7861 tggatgatgg taagctccat ttggtttatt taaaagattc ttctttgatc gatacgctta
     7921 aaacattgcc agacctatta aaaggagtag atgaatctac gaaaaatcta gcctacctta
     7981 cttgtgagca attagttgtg tcattagctg atgaggaaga attgacgacg aatgttgatg
     8041 gagatgaagg ggataaatta cctgtgacaa tcaaagtatt gccctcacat ttacaggttt
     8101 attgttaaaa tggaggttgt gaccgaggag ttggcgggag aacaccgctt gttcgggata
     8161 agggactgtg acaagacttt tgtcacagcc ttttttttgt acaaaaaaga aatttacacc
     8221 taatagaaaa atttttccat aaaaatacat attttaaata tgaaagtaca attggaaata
     8281 gattaaaacg ataaaataaa attgtcaaaa cgtcagagag gatggtggag aatgaatcaa
     8341 tgtgtaaaaa agctgattgc gtcgattttg tttgtcttcc ctctcttgat ggtgacatcc
     8401 gtttttgccg atgtagagaa aacaaacgaa tcaacgcaat cagagaatca aagttttgac
     8461 aagactactt tttcagaaga atcgattact tctgaaacta cagaatctca aagtgaggct
     8521 tctactaata aaaggcaaga agaaaataag acaagtgaga cctcagaagt agctgaacaa
     8581 acgtctgata gcagtgaagc ggcactggca agagctgttt cagcggatgg gtacagcgaa
     8641 gctgccacaa cagaagaact ggcgcagctt ctcgcagacg aatcagtaac taagatccga
     8701 ctgattcagc ctttgacttt agacagggaa ttagagatca aacgagatat cgttattgat
     8761 tttggtggat tcgctcacaa tttcggcaca caccatattt atatcaatga gatacctcag
     8821 tcgatcgagt tccagaattt taaaggaaca gctgcacatc caggtggatt gacaccaaat
     8881 atcgatggaa atgccattat cattgcttat atgaccgatt tttggggaag cagatatcat
     8941 tttacgggag aaataaaatt taccgggaca cttgatttgt atgatgggtc caaattagga
     9001 ctgatttacg ctcctcgtgc aactgtcaca ttagatggtg tttccggtgt gttggatgtc
     9061 caaccagtga aggaaggact gaatgccgct ccgggtaaag cctatttttg tagaagctat
     9121 caattgaatg tgatcaatgg tagtcaactt tatggaccgt atctagggaa attctacggt
     9181 tttctagatt caggagatga gactggtagt ggaaaaacag cgccaggaat ccatatctta
     9241 agcggttcaa aggtctctct tgattatgat cgggcagatg gcgttagcga tgaaggagag
     9301 gcagtcgata cgctacctgg taatgtcatg tttgaagtca gtggttcggg aagcgaattt
     9361 tcggtaaata caaagatcaa tattaccaat gacaacaatc gcggaatcat ccaaatgaga
     9421 ggttcaggat cgaaagtcac tgtatcgaat ggtgggaaaa tcaccatcaa tacagcaact
     9481 acgggcggat ttcgcttgca aggagattct agtcaaatcc atgtatcaag tggtggacaa
     9541 atctcggtca aaatggctgg agatgacgat caacccggga acaatgggat gcgttttgta
     9601 ggaaaaggcc tttctttgat cgtagaagga gccggatcag ttatcgacat cgataagcag
     9661 agtgggcgtt ccagcgcagt tcgttttgag aatggtacgc aaaagctgac agtaaccaat
     9721 ggaggctcat tgagggtaag aaatacaggt gatggcaaat cttacgtgaa cagactcaat
     9781 caaggaaatc aagcgatcca attttatgac gcttctggtt ttctagaaag tccaggttca
     9841 gctgatttta cagtcactgg agaaaaatca aatatcgata tacgcgcaga ttccggagca
     9901 actgtagata caactggaga tattgattta aattttacgg caggtgaaaa aacttattta
     9961 gtcatggtcg ggaaaacagg ggaagataag accggcatca tcagtgggga tgtgttgaat
    10021 gtggacttag aatctcctac ctattttgat ttccaaaaca aacggattgg aagcgagacc
    10081 aatggcatag gcggctgggt attccaagga aatggagatt cttctttaaa gatcgcccat
    10141 tcagaaatcg ctttatggcg aaaaacaggc gcaacattcg ataactttga aaaagacccg
    10201 gattattttt ctgatatgac agacttatcg atcaatggga ccgacttagg caatttcgta
    10261 tcaagcccta gtgcaggttt aacaaatgaa tttgctcaga caggtgccgg aatgaaaaat
    10321 tataatcgaa tcagtgccaa taatcagctc ccgatgattg aatccttaag agtgccaacg
    10381 aatgcagata aaaaaatcta tgggcatgtc gttgttcccg aaggtgtcga ggcaaagcca
    10441 agagatgctt ggacaggcga agtagaagtg actttacgta ttacttacgc agatacgacg
    10501 aaatcatctg tagaaattac tgctctcacg caaggacaaa cagatgaaaa tgaaggggca
    10561 ggatataacc catggggaga aggcgaacaa ggcggcttgt tcgaagttca agtacccaat
    10621 gatgagagat taaaaacgga ggatcaagtg caagttgttg ctgctaaaaa agtaagaggt
    10681 ggccaagaag tgacagaact ggactctgcc aaaacgacag tcgatgtgat tcctcctgaa
    10741 cctgctaatt taagtaccac attgatcaat gcagcttctc gcacgatcac aggtacaggt
    10801 gaagcaggag ccttggtttc attgaaaaaa aatcaagctg ttttgtcaga aacaactatt
    10861 gatgatacag gggaatttgt tctttctatc ccagaaaatc aactttttgc aggagatgta
    10921 ttagaaattg tattaaacga ccaagcagga gaagctactg ctaagggtgt gcagaacaag
    10981 ccaagtacca acgatgaaat tgggaatcac aatccaagtg atacgccagt tgtttatcat
    11041 gatgccccgc cttttgcacc tgctacgaag atcgtagtag aaggtggtct ctcatttctt
    11101 gccccttcca aactcgattt tggtcagatc aaagcaactg gtttgacaca agaagcattt
    11161 ggccaaatgg atatacagga aaaattatgg atttatgatc agcgagaaac gaaaaatccg
    11221 tggatcttaa cgataaaatt atctcagcct ttcacaaatg atgcaggatt ttctatgcag
    11281 gatgtcctat atttcaagaa aggagaggag tatcaattag tgaatgaaga agaactggaa
    11341 attgtcagag gacaaaattc aacgaatgtg caagcagatt attcagaata tttaaataac
    11401 ggtcgggcgt ttaaacttgt cttaaccaaa gaaaatcaga tagctggtaa ttactcagca
    11461 gagataacat ggacattagc agatgcgccg acagaataaa gatcaaggag gatgattgat
    11521 gaatcgaata tggaataaaa gtcatcgaaa caactgctct ttacttatga tccttctcct
    11581 tggattcatc ctttggcaga cacctgtcca tgcctccgaa ttcaattttg cggtagattt
    11641 tgaattaccg gctaatcaga ttgataagaa caaaacatat ataaatgtaa ggatggagcc
    11701 tgggaaaaca caggaaatcc cctacacggt ctttaaccga acagataaat cagtaacagt
    11761 tgagacattg atccactccg ctacgacgaa ttcaaacggt gtgatcgaat atggagaatc
    11821 aaaaagtaaa tttgatgaaa cattaagtta tgatatgaaa aacttagcaa aaacagtggg
    11881 agaaataact gttcctcctc aaagtgcggt cactgagaca ctttcactga aagcgccaga
    11941 aaaagagctg gctggtgtgg tcgcaggagg gatcacgttt caagaaaaaa aagagaacgt
    12001 ttctaacaaa agtacagttg gtgtcgtcaa tcagttttct caagtgaaag caatcttgat
    12061 ttgaaatagc gaaaaagagt cttccccaga tttgaaatta aaaaaagctt atgcggatca
    12121 aagcgatgga agaaatgtga ttaaggcgaa tcttcaaaat ccaaatgctt cttatatgtt
    12181 cggcttgact acatcggttg aaatctacaa aaaagaaaca aaagagcctt acctttcaat
    12241 aaaaaaagaa aaaatagatg tagcacccaa tacaaatttt gatttacttg tcccattaga
    12301 aggaaaaaaa ttagctccgg gacattatca agcggttgtg aagggtgctt ggaaagaaaa
    12361 acgctggaat ctccataccg attttgaaat aactgatgat gcagcggacc aattgaatga
    12421 aaaggatatt gatttaaaaa atcagccaga aacatttgac tggcgatggc tgatacttgt
    12481 tggagtcatt cttattggct tatttgctgg attatttatt ttaaaagaaa gaaggaaaaa
    12541 gaaatgaaaa aaacaattgc attaagtacc gttctagctg gaagtctatt attatgtaca
    12601 caaagtgttt ttgctgagaa taagagctca gccactacag aaggaacgat ttctgtgacc
    12661 aaaccagatg ctgcaaatcc tgaagacgtt gttccagttc ctcctgttga tccaaatgat
    12721 cctgatactc cttttacacc tgacgaacca aatcagggaa catccggatt actgacgatt
    12781 gaccaagctc cagatcttga ttttggaaca atcaagttag gtacagcaaa aacagtatat
    12841 gctgcattac aaagcggaac ggacagtact ggagcacaaa cacaagtaca gaatttcgta
    12901 caagtaacgg ataaaagcgg aaattatgcc ggttggaaat tatcagtcaa acgaacagaa
    12961 tttattggta caacatctga tacgaataaa ctaactggtt caaaggtgaa attcaaaaac
    13021 gccgtgattc gaaatagcgc aactaatagc agtggaatgc catctaccgt ttctggaact
    13081 ggcgctgaag gaatcgaaat cccagcagat caggcagtag atctagtaac tgcaggagca
    13141 aatgaaggga ttggcacatg gatttattca ttaggagaga atgtggaaca agggagacaa
    13201 tcagtggaat tatttgttcc tgtatccaat tatgctgtag acaactacgt ttcaacattc
    13261 acatggtcgt taacagatgc tcctacagaa gaggaagcgt aaaatcatat caacgatttc
    13321 agggagtgga gccgattcaa ggcccactcc ctgaaatctg ctatgcttac gtctcagctc
    13381 ttattcatca actcgaagca gttttttgga agtcatggaa acgttccttt gtcttttcaa
    13441 agatctttcg accaacgagt aaggagtcac cgttgaaaaa ttctaacaca taatgagcag
    13501 aatcaatttt ctgcaattgc gataagttga tacagtaaga ttttaaaagt aaaaagtaag
    13561 catacttttc taactctgtc ttcaattgtt ttaaagaagt attcactaat ttatcttctg
    13621 tagttgtttt gaccataact tggtttttca aacctttgat actttcaata tataaaattt
    13681 cccgaggata caggacgaga cttccacctt tttgatcaat aaagatttta tcgacattta
    13741 cgatctgctg ttctttccaa ttgatctgtt ctagcacttg acgaatttgt tcagataaat
    13801 tcctgtcttt tctgatatat ccaatcggaa ggattttccg attgatgatc tctactgcaa
    13861 aattcgtata agcggtgaaa aaaacaatat tactatgtgc atttttttta cgtatttgtt
    13921 cggcgacatc aatacctgtg agtgattgct tcaagtcgat atcgataaag aaaacatcgt
    13981 gattatcaat agtgcccaac tggatcaact gattgatttg agagatagaa gtgagctgca
    14041 cgatattctc gatatgatga ttgaccaatt ctctgatgat ccattcgctg tgaaactggt
    14101 tatcttctat gataaaatac tttttcatat gcaggctcct attaaaaaaa tatgtgtaca
    14161 ttttgaaaca aaacaatgga ggaataagat ggtcgcaaca gtgacacttt atttgacctt
    14221 tactttgatt ttagatttag gtatctattt attcttttcg aaacaactgc tagtaaaaaa
    14281 cagctttggt tgtatagctt tgatggtggt gacactttta cttcctatcc ctaaagtgag
    14341 ctttccttac ctagttggct tattattgat gtttcttttt tattttacta cgaaaaacct
    14401 gtttatcagc tgtttgatca gtaatctttt ttttgctatc actggactca gcttttttct
    14461 ggtttttgat ctgatggatt atttaggaaa gagacatctt ggaacagcaa gtttttttgc
    14521 actacttatc gcatttattt tctatctatt agtaaaaagt atagacaaac agacgagatt
    14581 tctcaacttg ctttttactt atccaactga acactatttt ggaggtattt gtattttatt
    14641 aagctggggc gtgatttact tggtattggg aagtgttcac ttccgctcat ctaattatct
    14701 gcttctatct cttttgtcga tagtcatcag ttttttttct ttgttttttt ccattatgtt
    14761 gatcagtttc caagtaaaag aaagacaaca tcaggaatcg cttcaactgt atcaaataaa
    14821 tgaagaatat taccatcatc tagaggaatt caaacatgat tataaaagtc tactgttttc
    14881 attaaaaatt actcttcagg aaaataaaaa agaaacgttg gaatttttag aaatattaga
    14941 agatcattct gcagctattt ttgaaaaacc gcaaatacaa cagctaacaa atattgtatc
    15001 cccagctgtc aggggtgtct ttttaaaatt tctagaaaaa gcagaatcgg agaaaatccc
    15061 gatccaagta gttattcctg tcgaagtgaa aacaatcccg attgatctgg ttgtttttat
    15121 tcgctgttta tctattttta tttctaacgc attcgatcat cgtgtagccg atcagagtcc
    15181 gattacgatc aatctgctgg aagaaaaaga gggatcacgt tttagtatag cgaacaaggc
    15241 aaatccaacg aatttgacgc tatcggagat ggtcaaaaga ggaaaaacct ctaaaaaaaa
    15301 tggcgggtta ggtctataca gtgcgaaaaa aatgttggat gtttacgaaa atgcagaact
    15361 aaaaatcgaa ttttcaaaac agaatcacta tttttttgta gaaatgtatc tggcatctag
    15421 aaagagcaaa tcagcctact gacttactgt caaaggataa gtttgaggaa agtctacacg
    15481 gagatagaga caacaacaca gcatattcaa aagaaacaga agcgtgatga ccgttgtcac
    15541 ccttctgttt cttttgctat gtagttaaac aaagtagttg acacttccgc tacttagtat
    15601 tagtacgaaa gtatacttca tgatactaag tagataagaa gtgaaaagcg gaacccacga
    15661 tcgtcaaaat gctaactgct ttgcttacac cagataaagg gaagagcaat atcgcttatt
    15721 ttaggcgtgg agaagcaagc aaatccctac gatcacaaaa aaaatgctga cccagcagtt
    15781 aagagagtta gaacaagatg aaagtatcca tcgaaaagcc tataaccaag tcccgcctaa
    15841 agtagagtac tcgctcaccg aagaaggaaa atcattaaga gagattctag tagcgttgtc
    15901 tgtttgggaa gaagaacgta tcgaaaaaca aaaagcctca agggaagaag acacgctact
    15961 gcatacacat ggtggctacc taaattattg attttctatg acatttattt taaataaaag
    16021 aaaacaggag tttttttaaa agcatcatct attttttatg taaaaaaatg gtatgattaa
    16081 tcaagtaaga agtgattgga agaaggcaag caaatgaata gtatggaaaa agtcaaccag
    16141 tttagagatg aacgtaattg gcgacaattt cataacgaaa aagatttggc tatctctatt
    16201 tctttggaag catctgaatt gttagaactt tttcaatgga aaacgccgga agaagtaaaa
    16261 gaaacacaat tagaacgaat caaagaagaa ctggcagatg tattgatcta ttcatacatg
    16321 atggcagaca accttcacct ggatctagat gagatcattg aagaaaaatt gatcaaaaat
    16381 aatttgaaat atcctgtaga aaaaagcgca ggtgtacgaa aaaaatacac agagttgtag
    16441 aaatgtgtca ttcggctgtt ggaaagagca aaagaaaaac agtaaaatga gtctggaggg
    16501 agagaaaatg aaaaaaaaag gaaaagcaaa aagatggcta gtcaatatcc tcttgttcct
    16561 cttgttatta gtaggactag ctttgatttt caatgaacag atcaaggatt attttgtcag
    16621 agagacagga gataaatatg ctatagcgaa tgtaacaaaa gaagatctga aaaagaataa
    16681 cgataaagat gtcagttttg actttgatgc ggtagaacca atgacgacag aaggggtcat
    16741 gaggtcgcaa atgagaggaa cagatctacc agtgattgct agtatcgcag tcccttcagt
    16801 ttcaatcaat ttaccggttt tcaaaggctt ggataatacg tcactacttt acggagcagg
    16861 aacattatct ccagatcaag aaatgggaaa aggaaattat gccttagcaa gccatcgtgc
    16921 aacgaaccct gaattgctgt ttacaccgct agaaaattta gagatgggtg ccaagatcta
    16981 tttgacagat ttagaaaatg tgtatacata caaaacattt tttaaagaaa aagttgctcc
    17041 tacagatacc caattactga atgaagtcga gggaaaagaa attgtcacac taattacttg
    17101 tggcgacatg gacgccgtca cgcgactagt tgtacaagga gaactggaaa gtgttacatc
    17161 gataaaagat gcgacagatg atatgagatc tgctttcaat ctagagacaa aaacatttta
    17221 ggatactggg acataagtcg ttacagatta aaaacagcca aaggatatga atcgataagg
    17281 aaaaaccaat ttgctgaaaa aaagagaaac gcaaagaggt gcatctctga tttggttgtt
    17341 ttctagatag acttgtgttc cagttttttt attttttata tgaaaaggct tgcataaata
    17401 aaaattcgat ggtatgatga ttttgaataa gaaatggatt gttactgtac ggcaggcaaa
    17461 accagaattc agctaagaaa cagtaggcga attctaggtt tttttatttt tcttgagagg
    17521 gcgaagcaaa atgaaaattg ataaaatttt gaataacaat gtcgtcattt cgaaaaatgg
    17581 attcggggaa gaagtcgttt gtatgggaaa aggactagct tttcaaaaaa agattgggga
    17641 cgagatttcg ccagaagcgg tccaaaaaga atttgtttta agagattctt ttgctaaaaa
    17701 ccaattccag cagctgatgg ctgatattcc ggcagaggag atcgaatggg tcaaacaagt
    17761 agtcgaactg gctgaagata aactaaaagt tgaattttcg cctaatattt atctgacatt
    17821 gaccgatcat cttcattatg cgatcacacg agcaaaagaa aatatccaac tgcctaatcc
    17881 attattattt gaaacaaaaa aattttatcc gaaagaatat caagcagcga aagaggctct
    17941 tttgttcgtg aaacaaaaga cgggtgtgga actttctgag gatgaagccg gttttattgc
    18001 ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg caagtgacga tgaccgctac
    18061 cacaatggta agagatatac tgagtatcat cagtaaattc ttcggcatcg tgttcgatga
    18121 agaatctttg aattatcagc gtatcattac tcatctgcag ttttttgccc agcgttatct
    18181 caaaggggaa ggatctgacg agcaagatga atttctctac gcattagttc aaggaaagta
    18241 tccaaaagcg tttcattgtg tcgaacggat caatgagtat cttcgacaaa cgcaaaaaca
    18301 ggaaatgggg atcgcagagc agatttattt aacgattcat attcaaagag tagtaagtga
    18361 gaaaaaaagc atccaatcat aaggattgtt actgtatggc aggcaaaacc tggattgaaa
    18421 aaataggttc acgaatgttt gtgactgttt ttttcaatct gggtttttat tttttaaata
    18481 aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt ggtcgtcgtg tatgggaagc
    18541 ggtaggagga caaaagaatg tcaaaagttt agtacattgt gctacgcggt tgcgattcag
    18601 attaaaagat gaatctttag cagatacgca aaagttaaaa gaagacccgg acgtgatcca
    18661 agtcgtacaa agtggcggtc agtatcaagt agtgattggc agtaatgtag cagatgttta
    18721 tcaatcgatc gtggatgagg aaggtcttgc agtttcggag gatggaaaag aagagtccaa
    18781 aaatattttg aatcgtctga tcgatattat ctctagtata tttacacctt ttctaggggc
    18841 gatggcagcc gccggggtat tgaaaggatt cttatcattg gcaacggtga tgggctggct
    18901 gacagctgat agcggtactt atcaaatatt gtttgctgca gcagatggtg tctttacctt
    18961 cttacctgtc atgcttgctt ttacagcagc taaaaagttc aaagccaacc agtttttagc
    19021 agtagcaatt gctatggcac ttgtgtatcc agctatcaca gcagttgctg gtgcgggaga
    19081 agctatcagt ttctttggga tcccagtgat tctttcacca tctggttata cttcttctgt
    19141 cataccaatc attctagccg tatgggttca aagtaaatta gagccattcg taaaaaaagt
    19201 gattccgcaa tttctgcaaa tgattttagt tccgcttgtc gttttagtag tgatggttcc
    19261 attaacattt cttgctttag gaccaatcgg gacggttgcc ggtaatgctt taggcggtct
    19321 cttcaactcg atttatggat ttagtccaat cgtagcaggt ctaatcatgg gaagtttatg
    19381 gcaagtattc gtgatgttcg gcatgcattg gggattcgta ccgatcatgt ttttgaatat
    19441 cgaacaatat gggtttgatg tattgatgcc aatgcttctt ccagccatct tggcacaagg
    19501 tggggctgct ttagctgtag cattgcgaac aaaagatacg aaacttcgag cattagggat
    19561 ttcttctaca gtgacttcgt tatttggaat cactgaacca actgtgtatg gtgtgacatt
    19621 acctttgaag aaaccattca ttgccgcttg tatttctgga ggtattggtg gagctattat
    19681 cggattttcc ggagtaaaag ctttctccag cagtttggtc agcttattga cgattccgac
    19741 tttcattaat acggtggatg gtgtagagtc aaatgtgaca gtagcagtaa tagctacagg
    19801 aattgctttt gttcttgcat tcgtaggtac attgatttta ggatttgacg aacaaacaca
    19861 agacaatcaa ttagaaaaca aacatgcgaa tgcaggagaa ccgattactt cagcacggca
    19921 taccttaaaa agtcctttaa cgggtaaagt actgccattg agtgaagtac cagaccaagt
    19981 tttttcttct ggcgtgatgg gaaaaggtat cgctattgat ccagaagtag gagaactagt
    20041 tgcacctgca gacggagaga tcacgactat ttttccaact ggacatgctg tagggatcac
    20101 aacgactgac ggagcggaga ttttgattca tatcgggatg gatacagtag aattaaatgg
    20161 gaatggcttt gagatcttag tcaaacaagg ggatctcgtc aaagcaggag atttattgat
    20221 tcgttttgac atcgaagcaa ttagagcagc tggctatagc gtaatcactc cagttgtcat
    20281 tacgaatact gatgcatttg ccgatatact tgaattagac caaaaagaga ttattgcaaa
    20341 cgaagatgtt ctggcaattg taaaataagg aggaaatcag gatgcaatca cgtttttcag
    20401 aaaaattttt atggggtgga gcaactgctg ccaaatcaat tagaaggggc ttatgatcga
    20461 gacggaaaag gactatccgt tgcagatgct atgcctggag gtaagcagcg ctttgcgatt
    20521 atcggcagtg aagaatttga ctggacgatt gatcaagaaa aatatattta cccgaatcat
    20581 cgaggaatcg atcattacga tcgtttcaaa gaagatcttg ccttgtttgc ggaaatggga
    20641 ttcaaatgtt atcgtttttc gattgcctgg acacgaattt ttccaaatgg agacgaaggt
    20701 actcctaacg aagcgggatt agaattttat gatcagctga ttgatgagtg cttaaaatat
    20761 gatattgaac ctgtgatcac gatttctcat tacgaaatgc ctcttcattt ggcaaaagaa
    20821 tacggtggct ggaaaaatcg aaaactgatc gacttctatg aacgatttgc tcagacagta
    20881 ttagagagat acagcagtaa agtcaaatat tggatgactt tcaatgaaat caattcggcc
    20941 tttcactttc cagctttaag tcaaggatta gtaaaaagca atggagcagg agagtaccaa
    21001 aatattttcc aagcatggca taatcaattc gttgcaagta gcaaagccgt aaaaatcggc
    21061 cacgaattgc gttcggatat ccaaatcggc tgcatgatta tctatgcaac aacttacagc
    21121 atcgatgcca atccagtcaa tcaggcagca acaatgatcc aaaaccaaga attcaatttt
    21181 ttctgcacgg atgtccaagt acgtggagaa taccctgcat acacagcgcg tacccataaa
    21241 aaatatggag tcgatccgga aaaattagag caaacggaaa aagacttcca gttgctaaaa
    21301 gaatatccag tggactacat cggatttagt tattatatgt ctacggcaat caatgagact
    21361 gatccacaag cagcaacttc agaagggaac ttgctgggag gagtaaaaaa tccgtttctt
    21421 gaggcaagtg aatggggctg gcagatcgat ccagaagggc tgagaatcgc attgaatgaa
    21481 ttatacaatc gttaccagaa accattattc attgttgaaa acggtcttgg agccattgat
    21541 catatggaag aggatggcac gattattgat gattatcgga tcgattattt acgtcgtcat
    21601 atcgaagcga tggctaatgc agtagcagac ggtgtagatt tgatgggcta tacgccttgg
    21661 ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga gtaaacgtta cggctttatc
    21721 tatgtggatc tagacgatga aggaaatggg acactgaatc gatcaaagaa aaaatctttt
    21781 gattggtata aagaagtaat tgcctcaaat ggcgtaaact tgtaattaaa gcagctgaat
    21841 gaaaaagaac acacttctca catgtgattt gtgttctttt tttatttttt atgtgctatt
    21901 acttttagta atagtaaagc aaagggtgac gttatgaaaa ggacacgtgt actatacttg
    21961 gaagtcgcag ataaaattaa agaagatatt ttctcaggaa aatatccagt cggttcgatg
    22021 ttgccgacag aaagtgaatt agaagaactg tttcaagtca gcaaaatcac tgtccgaaag
    22081 gcaatcgaac tgctggcatc agatgaatat gtcgcaaaga aaagtggtcg tgggacgacg
    22141 gttctaagca atcgtcctta caatcgatta tccaaagcgg caagctttac gcaaatcttg
    22201 gagaactccc gatatgaagt ccggaaagaa acgctggatt tgcaattggt ccatataaag
    22261 aaggaagatc ctttgttttc ctttttcggt gagcaagctg tttgttttaa gcgcttatat
    22321 tatttgaaca atcagcctta tatttatttt gttcattatc ttccacaaga aatgatgcaa
    22381 ttcagcagag aagaatttgg caaagaatct ttgtatcgtt tgctgacgaa aaaaggatat
    22441 actattgact cattttctga tgatttctcg gcagtatttt taacagaaga agaacaagga
    22501 attctgaaaa cagacgcaca gatcggcatc aaacggatac ggaaatcgtc tacgcaaatt
    22561 ggggaaatga ttgaatattc ggaggcggtc taccatacgg agctgcatcc ttaccatatt
    22621 gattatgaaa cataaataaa
//
LOCUS       DACEOE010000028        22563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEOE010000028 DACEOE010000000
VERSION     DACEOE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.029"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(34..753)
                     /locus_tag="IW774_000928"
     CDS             complement(34..753)
                     /locus_tag="IW774_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1153291.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            1090..1422
                     /locus_tag="IW774_000929"
     CDS             1090..1422
                     /locus_tag="IW774_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153292.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            1409..3412
                     /locus_tag="IW774_000930"
     CDS             1409..3412
                     /locus_tag="IW774_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1153293.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            3431..3901
                     /locus_tag="IW774_000931"
     CDS             3431..3901
                     /locus_tag="IW774_000931"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1153294.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            3922..4506
                     /locus_tag="IW774_000932"
     CDS             3922..4506
                     /locus_tag="IW774_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1153295.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            4521..5507
                     /locus_tag="IW774_000933"
     CDS             4521..5507
                     /locus_tag="IW774_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1153296.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            5497..5808
                     /locus_tag="IW774_000934"
     CDS             5497..5808
                     /locus_tag="IW774_000934"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1153297.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            5830..7611
                     /locus_tag="IW774_000935"
     CDS             5830..7611
                     /locus_tag="IW774_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1153298.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            7604..8980
                     /locus_tag="IW774_000936"
     CDS             7604..8980
                     /locus_tag="IW774_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1153299.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            8987..9622
                     /locus_tag="IW774_000937"
     CDS             8987..9622
                     /locus_tag="IW774_000937"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1153300.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            9652..11007
                     /locus_tag="IW774_000938"
     CDS             9652..11007
                     /locus_tag="IW774_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1153301.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKFAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(11199..12593)
                     /locus_tag="IW774_000939"
     CDS             complement(11199..12593)
                     /locus_tag="IW774_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1153302.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            12685..13656
                     /locus_tag="IW774_000940"
     CDS             12685..13656
                     /locus_tag="IW774_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1153303.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            13653..14434
                     /locus_tag="IW774_000941"
                     /pseudo
     CDS             13653..14434
                     /locus_tag="IW774_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(14603..14974)
                     /locus_tag="IW774_000942"
     CDS             complement(14603..14974)
                     /locus_tag="IW774_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1153304.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            15108..15551
                     /locus_tag="IW774_000943"
     CDS             15108..15551
                     /locus_tag="IW774_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1153305.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(15594..16403)
                     /gene="yidA"
                     /locus_tag="IW774_000944"
     CDS             complement(15594..16403)
                     /gene="yidA"
                     /locus_tag="IW774_000944"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1153306.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16422..17789)
                     /locus_tag="IW774_000945"
     CDS             complement(16422..17789)
                     /locus_tag="IW774_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1153307.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(17773..18603)
                     /locus_tag="IW774_000946"
     CDS             complement(17773..18603)
                     /locus_tag="IW774_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1153308.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            18766..19203
                     /locus_tag="IW774_000947"
     CDS             18766..19203
                     /locus_tag="IW774_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1153309.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            19279..19899
                     /locus_tag="IW774_000948"
     CDS             19279..19899
                     /locus_tag="IW774_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1153310.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            20088..20594
                     /locus_tag="IW774_000949"
     CDS             20088..20594
                     /locus_tag="IW774_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1153311.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            20611..21510
                     /locus_tag="IW774_000950"
     CDS             20611..21510
                     /locus_tag="IW774_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1153312.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            21597..22482
                     /locus_tag="IW774_000951"
                     /pseudo
     CDS             21597..22482
                     /locus_tag="IW774_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
BASE COUNT         7308 a         3923 c         4847 g         6485 t
ORIGIN      
        1 aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa agaaatcgaa
       61 taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt ggacaccaag
      121 gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt tggaaaaggc
      181 gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg aattcacaaa
      241 gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga cttcgtgagt
      301 tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg gatataatga
      361 aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc ctcggcagac
      421 tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt cgaattggtc
      481 tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa gttgcggact
      541 aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt gaggcgctga
      601 atctggagac atgactggca gaataagagg taatccttga gcatctaaaa ctgcatccac
      661 aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg ctcgaatcag
      721 ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag tagactaatt
      781 gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact tatctgttct
      841 tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat agttgaaaat
      901 attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc gtaagcttca
      961 aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg cctatactaa
     1021 gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc ttttttaaat
     1081 cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc agaagaagaa
     1141 aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga agaaaaagag
     1201 caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt ggtagaagaa
     1261 aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa agcagaagca
     1321 gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca agcaatcggt
     1381 gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa aagtgaccct
     1441 catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa tccaaaatgt
     1501 tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa aatattttcc
     1561 agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc ggctgaaaga
     1621 catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa agaaactcca
     1681 tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg aagaaaattt
     1741 catgaataaa ttagaagagg tccttgattt aaaagaaaag tgggaacagc taactgaaaa
     1801 acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc tggatattgc
     1861 tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga gtgcagcaaa
     1921 ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg aagaaataga
     1981 ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag ttgctgctat
     2041 agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg aacaaaatcc
     2101 taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc agcaaaaaaa
     2161 gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat gggtagaaga
     2221 agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc atgccactta
     2281 tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga tgcatgtgtt
     2341 aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa actcagaaga
     2401 gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac cttttgaatt
     2461 gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc cttggatgac
     2521 accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg gattgctgat
     2581 gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga tgaaacgttt
     2641 cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga tctacagttc
     2701 atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt tcccaatcct
     2761 atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg gtctgataca
     2821 gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag attacttgag
     2881 tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta ttgcattgat
     2941 tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc tatcgatcat
     3001 tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag tcaagggtgc
     3061 tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt tagtcagtta
     3121 tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat tcaatatgct
     3181 agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga tcatcgcttt
     3241 acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg cacgattgca
     3301 gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg acccgctgaa
     3361 aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat aattggagga
     3421 tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat ttgcagtatt
     3481 agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg ttggaatgac
     3541 aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac aggctttgat
     3601 tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct ttttgatttt
     3661 tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag gcgcttctct
     3721 gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg cagcagcagg
     3781 tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat
     3841 ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat tgaatgccta
     3901 agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca gatgaatgaa
     3961 gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat cgatcaaaac
     4021 tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga aaaacaaatc
     4081 gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga agtacgacaa
     4141 gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc aatagctgaa
     4201 atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt gtattcatta
     4261 cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta tctttctcag
     4321 acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag tgatgaaact
     4381 gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa ttttcttttc
     4441 agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa tcaattattc
     4501 gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt cgcggaagag
     4561 agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat tctgtcgaag
     4621 cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac ggatatgaat
     4681 atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg ttaaaggaat
     4741 cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc cataatctga
     4801 aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat atcgacgatg
     4861 gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca aatgaactcc
     4921 ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag tctaccattc
     4981 ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga cggcttgccg
     5041 ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac ttgaccaata
     5101 tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg acgacggtac
     5161 tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat aaagatatgg
     5221 aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc cctgtcatcc
     5281 gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat ctaagttcat
     5341 tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc tttttgaacg
     5401 caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga agccatttcg
     5461 aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat cggtgtagtt
     5521 ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg ttatggtaca
     5581 accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt
     5641 tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta caaggaacaa
     5701 atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat agggctggaa
     5761 gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta tgaaaaggag
     5821 atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt aatggctgag
     5881 aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg agttatcgga
     5941 gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga gacttctgga
     6001 attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga attaggacca
     6061 gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt tatggaaatc
     6121 acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca tgagaaaaaa
     6181 tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga tgttatcggg
     6241 atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg gataaaaggt
     6301 acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg tgtcattgaa
     6361 accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg
     6421 ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg agtgattgat
     6481 acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt tggtgcaggg
     6541 aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt tgtctatgtg
     6601 ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc tgaattgatt
     6661 gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa tacatcaaac
     6721 atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc ggaatatttc
     6781 cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg ggcagaagca
     6841 cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta tccagcttat
     6901 cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc attaggatca
     6961 gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat cgccgtctgg aggggatatt
     7021 tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg tcttgattcc
     7081 agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta ttctctttat
     7141 tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc atccatggtc
     7201 accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt gcgtttggta
     7261 gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa atccattcgt
     7321 gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc aagagagaaa
     7381 caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg
     7441 ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga aaggatcgga
     7501 cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat tggtgaaatc
     7561 aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta aagaatatcg
     7621 tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg gtgtcaaata
     7681 tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga
     7741 agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta tcaatttacg
     7801 tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg aagacatgat
     7861 cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga tcctgcctga
     7921 aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt atccagatga
     7981 gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc gtgggcaaaa
     8041 attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg
     8101 acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg ctattggaat
     8161 cacttttgaa gaagcagaat ttttcatgga agattttcgt caaacgggcg caatcgaccg
     8221 ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gagcgtatcg ctactcctcg
     8281 tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg ttttggttat
     8341 catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag cacggcgaga
     8401 agttccagga agaagaggat atcctgggta tctttacacg aacttagcta ctttattcga
     8461 acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga ttctaacaat
     8521 gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta cagaaggaca
     8581 gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg atgtactccc
     8641 gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag aagaccatgc
     8701 agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa aagaattggc
     8761 cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga agtttgctga
     8821 gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga ttactgaaac
     8881 acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa aacgaatcaa
     8941 agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg cacgattgaa
     9001 tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa ctgccacaag
     9061 agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca tcctgttgat
     9121 tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag caatgtctga
     9181 ttttgtattg gcaaatgctt cgatcacgga gcctttcatc gaagaattat ttgttttgcc
     9241 ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt
     9301 gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg ggtatttgaa
     9361 ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc caaaactttt
     9421 ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg aaaagacacg
     9481 tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa cgatctatta
     9541 tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa
     9601 aaatatgggt accactgact aagtggtatc cttttttaaa agaggaaaac gatgattaaa
     9661 caaagcaaac aaataaagaa acgcttgccg gctgttcagc ttctcgcatt aggatttttt
     9721 ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa tagtgggcat
     9781 gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt gacgggattg
     9841 acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat catgcttttg
     9901 attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc attgataaag
     9961 aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct agaagaagta
    10021 tcaggtgtga tgaagctcat gctttacatc ctgaaatttg cctttgtgat ccaactgatt
    10081 ggtacggttg ctttatcttt tgtattcgtg cctgaatttg gctggttgaa aggtatctgg
    10141 tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt gttaggagac
    10201 agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc tttgatcatt
    10261 tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca gaaaaagaag
    10321 ataacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt aggcggattc
    10381 gttgtgttct ttttcacaga gcgcaatggg gaaacgctag tggaagggaa tatgtttcaa
    10441 cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata ttattcagtt
    10501 gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat gtacatcggt
    10561 ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt gttgctgatc
    10621 cagatgcgtt cgatgcttaa aggacgtacg agagcggaag catttggccg taccatccga
    10681 caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt gtgtgtagtc
    10741 gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat cgaatatatt
    10801 gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt gacgccagac
    10861 ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg agttggtgta
    10921 ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat
    10981 ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta gtgatttagt
    11041 aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc aaaatgttcg
    11101 tttatttttt gaggcactat ccgttgttga acctcgggta gtgcctcttt cttattgaaa
    11161 ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata tttttttatt
    11221 ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga ggatagggaa
    11281 attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg
    11341 ataccgttag caagaaagtc attctcaatt atcttaggat caatctcagg atcaactgga
    11401 agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa
    11461 agctgattgg ctcttttcgt aagacttaat ttagtttcta agatcctatt tgcttcattt
    11521 gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac atttatatta
    11581 aaaattgctt gttctatctt gttttggaca tgtactggaa ctacaagata agcaactcgc
    11641 aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt aggtaataat
    11701 ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga atggatatca
    11761 tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc tcgtcttttg
    11821 tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac accatgaatc
    11881 ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc gtcaaaatca
    11941 attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc gtatgtatat
    12001 cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat agccaatcca
    12061 ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc ttgagttttt
    12121 aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg gtaattaagc
    12181 aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac gaatttatta
    12241 cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac ttcattggta
    12301 gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc aatattcgta
    12361 tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt aaaatttaaa
    12421 tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg taatttattc
    12481 tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg cttcaatttt
    12541 tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg cattttctac
    12601 actccttaaa attgtatacc atacaatttt accattgatt gtattgattt tagaagttaa
    12661 tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt agtagcttct
    12721 tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt
    12781 aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaaga caaaaatatg
    12841 tttgcgatga tgcctgctta tttgggggag aacagatatt ttggaacaaa agtcattacc
    12901 gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat tttcttgttt
    12961 gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac ctggattcga
    13021 acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc agaaacactt
    13081 gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc taccattcgt
    13141 cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac ttttatcgct
    13201 aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt agaagatgca
    13261 gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt tcttcaaaaa
    13321 gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc tgatacaaga
    13381 gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta ctcagctacc
    13441 ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga ggaatcttgt
    13501 attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa aggagaatcg
    13561 aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg tgcagaatat
    13621 atttataaca aattagagaa aggggcttta aaatgaactt agattattac gtagttgatg
    13681 catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc
    13741 tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa acagcattta
    13801 cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga gaaatcgatc
    13861 tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac aaaatacctg
    13921 atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga attaaagatt
    13981 attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg actgaatatg
    14041 aagaagcaat tggggctaag attaaagaag cctacttagc acgtgattta tttttcgtat
    14101 tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc aaaaattttg
    14161 aattaggaat agagtcatcg tgacagcaga aagtgtgcaa aaagactttg tttctagaac
    14221 cttctttcca aaattaacaa tcaatgaaga tccagtatgt ggatcagcac attctaatct
    14281 tattccttat tgggcagaaa gaatgaataa aacaaaattg tcagcttatc aaatgtcatc
    14341 aaggggcggg gatctagaat gtgaattaaa agaagatcgt gtgataatag gaggaaaagc
    14401 agttttgttt tctaaaggta cctcgtttat ttgatttctt tcaaatgaat taaaaataaa
    14461 ttggtggaga gtggcagcct tatagctaca atggttgtta aaaaaagacg caaacttttt
    14521 caatttaatg aaaagtttgc gtcttttttt aattattggt gtttgttttt tattcaatca
    14581 agagtaaaat gccccatact aactatcctc ggatgacttc gattttataa ccatcagggt
    14641 caatgacaaa gtagtaagaa ggcgtagtgc caggtaatcc ttttaagtca gtaatctcaa
    14701 gacctgcagc tttatgttgc tcgtgtaatt tttctaaatc atcagctgca atagcgatat
    14761 gaccatagcc gtctcctaga tcatacgcac catgatcata gttgtaagtc aactctaatt
    14821 catagtcatc tccaggaagt gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac
    14881 ggcggcggct ttcttcaaaa ccaaatgcct tttgataaaa ttcgattgat gcttcaagat
    14941 ctttcacccg aacacatgtg tgagccattt tcataaattc atctctcctt ttccttttac
    15001 tcaatcataa caaaaagaag taagaaaata gttgatttgt gcttgttcat gagaaatttc
    15061 tcatgtaaaa tggatagaaa ataatactgt taaggtggaa acaattcatg gataaaccaa
    15121 tttttggtga aaaaaagcaa tctgctaatt atcaaacccg ttatgcagca tatattgtca
    15181 tagaaagaga caagcggatt gcggtgatcg aggctccaaa tggtgctttt ttccttcctg
    15241 gtggggaaat cgaaggagaa gagacgaaag aagaagcgat tgtccgtgag ctgatagaag
    15301 aaatgggaat tgtggcagaa atttcttctt atctgggaga agctgatgag tatttttact
    15361 cgaattatcg tgcaacttat tattataatc caggatattt ctttgttgct gattcttgga
    15421 agcgtatagg agaaccaact gaaaagacca ataaaatttg gtgggtgagc cctcaagaag
    15481 cgcttgtgaa attgaaacgt ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa
    15541 ataaaaactg aaagaaagcg agtagatcag ctttctctca gtttttttat ttattacagt
    15601 acatattttt tgattgcttc agctacaccg tgctcgtcgt ttgatgaagt gatgacattt
    15661 gctgcttctt tgacaagaga aacggcattt cccattgcta ctcctagacc agcatattca
    15721 atcatggata agtcgttctc gttatctccg atagccatga tttcatcttg atcaatacca
    15781 aggtgctgtg cgagattcgc aactgccgca cctttacttg cttctttatt tagtacttcg
    15841 aagtaatagg gcgcactttt gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc
    15901 gcaattgctg catctaaaat ttctggttcg tcgatcatca tcattttgac gatgttcatg
    15961 tctggtgtca tttcttcagg tgttcgatat tttagcggca tcttgactaa acttgcttcg
    16021 tggatcgtat aagcgctgat atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa
    16081 tggagatgac tgccgacacg gcgtgccatc acttcgatct ctaggaaatc ttcatgagtc
    16141 agtccgtgac gggcgatgat tttacctgat ttcgtttgtt gtacgagtgc cccgttataa
    16201 gtaataacgt aatcattatc ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct
    16261 ggtaagggac ggccagtgca taaaacgatc gaaacacctt ttgcaacagc agcggcaatc
    16321 gcttctttta cttcttttgt tacttctctt ttctcattta ataaagtacc atcgatgtcg
    16381 atagcgacta gtttgataga cataatttcc tccaatatat tttaatttat ttcaatcaat
    16441 gcgccattac ggatatggct ggcaaattct tgataagttt cgtcaaataa atcataatga
    16501 tcacgcaagg agggatcaac catttctttt ggaaagaaaa atcgctcatc tccttgttct
    16561 tgtcccgcta atgcagcaac aagctgacta acctgtgata attctatcaa agttccgtca
    16621 tttcgcaaaa tctcgatttg tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa
    16681 ggcagatcgt agctagaatt cacagcagtg taataaacag ggttatagcc aaccttttct
    16741 actaacaaag tgagttcttg gactaatttt gaatcccggt tgccagaaaa agtagcagat
    16801 ttcagtggtt tacgattcaa aaaacgttta gctaaatcat ttaaaatagg atcagcagat
    16861 tcagaccatt gagtaaagta agtgcccaag accccatcat caagtttgag gtattcttct
    16921 aaagtaaagc ttccgtcaag aaatggcaga agtaattgag aatggagttc aaaactgctg
    16981 gcatcttctt gatacaactc gtgtgcgcga tgaagcagat gctccaaaat cacttccata
    17041 ccacgggaaa caggatggaa gtacacttgc acatacattt gatatcggct aacgatataa
    17101 tcttccactg catgcatgcc actcatggaa aaagcgatcc cgccttcata aggacgaatc
    17161 acacgtaata ttcgggtaag atcaaatgtt ccatattctg ttccagtgaa ataagcatcc
    17221 cgaagcaaat agtccattcg atctgcatcg atctgactag aaatcatttg gacaacttgt
    17281 gggttggggt aagttttttg gatcacacta gctacctttt caggaaaacc ttcttctact
    17341 cggttaagaa tctgatacac ttctgtttct ggagaagtga tgatctctac agtgatggct
    17401 tcatgattcg tatggaagat atgttcaaat gtatgagagt aaggaccatg accgacatca
    17461 tgcaacagtg ccgcacataa agtcaccagc cgttcctgat catcccagcc tccgttgcct
    17521 attttttctt ttgaaaaatt tcgagaaaag atatcgcaga ttcgtcttga aatctcataa
    17581 acgcctaatg aatgggtaaa acggctatgt tctgctccgt gaaaagtgaa agaagaagtg
    17641 cctaattgtt taatgcgtcg taaacgttgg acttctttcg aattgatcaa atctaagatt
    17701 actttatgct ggacatggat atagttatgg actggatcac gaaaaacttt ttccataggc
    17761 agcatttgat atttataagg ggttgtcata atgtagctcc tttatgttat tacgttgttc
    17821 cattctctgg atttggttgt tccattgctc cagctggatt tcttttttta gccagtcggt
    17881 agaatcctgt aaatcacttg cagaatacag cttttcaaaa gcttttgcaa atgcttcttt
    17941 gacttctttt acaaaaaggg gttggtcaag cagttcttca agtgttgcca ttgtagcagg
    18001 atccactgca ggatagccat ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg
    18061 cttcatgact tgcccgcgtt tttcctgatt accgttgaca cttaagtaca tcataacaga
    18121 aatcccattt ttgatccgac gctgagcgat accggcaaat tttttgccat ggatgcttaa
    18181 atcataagtt ccaggacaat aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg
    18241 aaaagcgtat ttagtcaata ggagcatttg ctcgtaagcg tcatctatgg ttattttttt
    18301 tgtgtcactt tgagggaaaa tcaaagaaat atttaaaatt ccttcatctg atacgatccc
    18361 taatccacct gaatttctga tcactggagc gtaacctgca tctttcagtg tttggatccc
    18421 gtcacttaga aaaggagttc gaacatcctt catacctaag atgactgtgc agtcatattg
    18481 ccagaaatgt aaaaagattt gctggtgttc tttagaatat tccgttaata tatctgttaa
    18541 tgcaaaaggg gaaaagtcgt ttttttgata aatgtgctga tctagtcgaa tcgcagggtt
    18601 cattcaaccg gctcctttgt ataaattcat attttctaca atattatact ctaaaatcaa
    18661 agagttgttt tggaaaaaag gttttctgta aaaatggaca agttggttga caaaacaaaa
    18721 gaaactagga acaatgtagc tatgagaagt aaaggagaat gaataatgga attatcacaa
    18781 ggaacaccga tttcaatcaa attgcgtaca aaagtaaaac agcaagggga agtgcaagat
    18841 ttttattttg atttgaaagg gcaaatggtg acaattggcg atacgctgta catacgttat
    18901 aaagagattc aggaggagac gggcgatgag attcctgtaa cgatcaaatt agttccagat
    18961 ggtcatgtac agctgatccg tgcaggagaa atgcggatgc gtttgaaatt tggctataaa
    19021 gaacggttag aaacatccta tcgaacacca tacggtatga tccagattgc tacttttacg
    19081 aaagaactgc atgttagttt gaaagaccgt cccacagcag ggaaggttcg aatcgattat
    19141 gatctgttca tgggaccgga aaaaatcggc gaatattatt tgaccttgga ttttacagcg
    19201 taaatataaa agaaaagttt gattttttgt gtctgatttt gtatgataag gagtagactg
    19261 tgaaaggacg tgtcctgatt ggaaattagt gtatttgaag gtgcaaacaa aagcgaatta
    19321 tcaatgatcg aagtagcaca cgcgatctta gaacaacgtg gcgatgtcat ggatttttct
    19381 gatttagcta accagatcca aaactatctt gagaaatcag acagtgaaat tcgtgattca
    19441 ctcgcacaat tttatactga cttgaatatt gacggcagct ttatctcatt aggggataat
    19501 cgttggggct tgcgttcatg gtatgctatc gattcgatcg atgaagaagt aaaccatgga
    19561 atggacgaag aggatgaaga tactccacgt cgccgtaaac gcaagaaagt caacgccttt
    19621 atcaatgatg atgaagacgc gatcgattac aatgacgatg acccagaaga tacagatctg
    19681 acagaagaag atgacgatga tctgtttgat gacgatgatg atgaagatga agaaatcgct
    19741 gcttataatt ctgacttgca agaaatcgga gcagatgacc cgacagatga tgaagaagac
    19801 ttgccaggaa atatcgaaga agatttaagt atcatagacg aagacgatga tgatgaagat
    19861 tatgaagagg aagaattttc tgactctgaa aaagaataaa cattattttc ttacgagagg
    19921 ttgtgacaga gacgatcagc ctatcagaat aaggaggtgt gacacgattt ttgtcacacc
    19981 ttcttttttg tatataaaaa tcccaagtaa atacataaaa aataaaaaaa ttgaaacttt
    20041 tagcgcacct tgtacgtcat attaagtgag gcagaaagga gtggagaatg atttttactg
    20101 gatataagaa aaaacggcag atcgaaaaat accgtcacct gctgaatgga ctcatcgaaa
    20161 ctgattatca aaaaatgttc cgtatcacga tgaattacgt acatgataaa gaagaggcct
    20221 tagatgtgat gcaagatagt tttcataaag ccttgaacta ttttgaaaaa gaaaaagaga
    20281 tcgagcattt tagtgcatgg ttttatcgga tattgatccg cacggcatta gatagttggc
    20341 gcagacaaaa aaggacgcct gtggacttat ttgaaattca agatatcccc caacagttac
    20401 ctttagaaac ttccgttgca gagctccata tgattttaga taaaatagac agtccggaaa
    20461 aagagattct gatcctctac ttttttgaag gatttcgttt gaaagaaatt gcaactatct
    20521 tggatatgaa tgaaaacaca gttaaaacga aaatgtaccg ctcattgaat gctttacgtc
    20581 aaatcctaga atgaaggaga gaagagtgcc atgtattccc aaaaagaaaa tttaaaaaaa
    20641 ctaaagaaaa cttataaaga acaagcaatt cccaaagggc ttaaagagga gatccgccag
    20701 cgttttatta tcgaagagca atcatttata aaagcaaggc ggagaaagag acgtctccaa
    20761 gggttcggag tatctttggc tatcgtaatg attacaacaa gtagccttct ttttaatagt
    20821 caagttcgaa gctttgcaga agatttaccc atcttaggat cagtcattga attgatctta
    20881 ggcgaacgct ttactgatcg atctgaaaaa atcgatattc aggtaccgaa gatcaataca
    20941 caaaatgaga aagaaaataa aacaattcat ggattaaatc aaaaatactt tcgcgaagga
    21001 caagcagatt ttgaaaaagc tgaaaaagaa tatggaaatt ttgaaacgga tcattaccaa
    21061 gttttgggag attatcagaa aatcgtagac gataaccggt ttctcgtgat tgaaaggcag
    21121 atcacccaaa cagcagcaga tagccatgtt gaaaaaagat acgacacaat cgacaagaaa
    21181 aacagtgtcc agctctcact tccactttta ttcaaagatg acacttatct atctgttttg
    21241 acaaaagaag tcaaacggca gatggccgaa caagtcaagg aagatccatc taagtactat
    21301 tggactgagc aagatattca agaagggacg atagaaaaac caacacttgt tacgcctaca
    21361 cgaagttttt atttgaacaa agagcatcaa ttagttttga ctttttctca atacgaaatt
    21421 gctcctggtt atatgggtac accagaattt gtcattccta agtccgtgac aaaaacgatt
    21481 ttggcatcgg aagattattt ggatcattag acgagaagta gttgcgacta gcagtttcaa
    21541 ttttcaagta agtacaagta aataataaga agagaaaaag agaaagaaga gatatagtga
    21601 aaagaaatta tttagtcatc agtattattg ctttatccgt gcttttgttt ttcgaaatta
    21661 cttcggtggc ctccttgatc accgaagtaa aaggacaaac acactcttcg gagcattcgt
    21721 cacaatcttt caaacaatca gaacaatcaa ccattaaaag ccaatcaact gaaacagcta
    21781 acgtcaaaga agccattcaa gaatcagcgg aaaaggtaca aagtcaatca tcacaatcaa
    21841 cacaagacag tacagaagca atccaagacc cgactgtcaa aagcgtcgca atcagctttg
    21901 atgatggacc aggagcgacc acaacgccgc agcttctgcg aatcttaaaa gaaaaaaacg
    21961 tgcatgtcac ttttttcgtc ttaggagaaa atacggcgca gcatcctgaa atcgtgaagc
    22021 agacagctga agcagggcac gagattggca atcacacata cgatcatcag aatctggcca
    22081 ctctttctgc ccagtcgatg acggaggaag tgacgaaagc agacactgag atcaaaaaaa
    22141 gtcactggca aaacccccgc ttttgttcgt ccgccctatg gttctgtcac gaatgtagga
    22201 gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca
    22261 aggaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg gtcaattatt
    22321 ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg
    22381 aaagagcaag gctatcgcat cacgacagtt agcgatttat tagggcatcc aacggctgtc
    22441 gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta
    22501 ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa
    22561 caa
//
LOCUS       DACEOE010000029        22479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEOE010000029 DACEOE010000000
VERSION     DACEOE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.030"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..175
                     /locus_tag="IW774_000952"
     CDS             <1..175
                     /locus_tag="IW774_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1153313.1"
                     /translation="DIASIWPVLLGAVVVCSIVTMVAAGKTAEGVEALLGYVRKKKAE
                     RVSVNEMEENQID"
     gene            168..869
                     /locus_tag="IW774_000953"
     CDS             168..869
                     /locus_tag="IW774_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1153314.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            869..2455
                     /locus_tag="IW774_000954"
     CDS             869..2455
                     /locus_tag="IW774_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1153315.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2474..2695
                     /locus_tag="IW774_000955"
     CDS             2474..2695
                     /locus_tag="IW774_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1153316.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2692..3453
                     /locus_tag="IW774_000956"
     CDS             2692..3453
                     /locus_tag="IW774_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1153317.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3833..4342
                     /locus_tag="IW774_000957"
     CDS             3833..4342
                     /locus_tag="IW774_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1153318.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4417..4956)
                     /locus_tag="IW774_000958"
     CDS             complement(4417..4956)
                     /locus_tag="IW774_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1153319.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5096..5707)
                     /gene="rpsD"
                     /locus_tag="IW774_000959"
     CDS             complement(5096..5707)
                     /gene="rpsD"
                     /locus_tag="IW774_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1153320.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6023..6820
                     /locus_tag="IW774_000960"
     CDS             6023..6820
                     /locus_tag="IW774_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1153321.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            6848..7483
                     /locus_tag="IW774_000961"
     CDS             6848..7483
                     /locus_tag="IW774_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1153322.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7503..8276
                     /locus_tag="IW774_000962"
     CDS             7503..8276
                     /locus_tag="IW774_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1153323.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8684..9481
                     /locus_tag="IW774_000963"
     CDS             8684..9481
                     /locus_tag="IW774_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1153324.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9459..9872
                     /locus_tag="IW774_000964"
     CDS             9459..9872
                     /locus_tag="IW774_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153325.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9856..12501
                     /gene="mutS"
                     /locus_tag="IW774_000965"
     CDS             9856..12501
                     /gene="mutS"
                     /locus_tag="IW774_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1153326.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12519..14627
                     /gene="mutL"
                     /locus_tag="IW774_000966"
     CDS             12519..14627
                     /gene="mutL"
                     /locus_tag="IW774_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1153327.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14649..15209
                     /locus_tag="IW774_000967"
     CDS             14649..15209
                     /locus_tag="IW774_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1153328.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15294..17282)
                     /gene="treP"
                     /locus_tag="IW774_000968"
     CDS             complement(15294..17282)
                     /gene="treP"
                     /locus_tag="IW774_000968"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1153329.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17505..18218
                     /gene="treR"
                     /locus_tag="IW774_000969"
     CDS             17505..18218
                     /gene="treR"
                     /locus_tag="IW774_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1153330.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18295..18741
                     /gene="msrB"
                     /locus_tag="IW774_000970"
     CDS             18295..18741
                     /gene="msrB"
                     /locus_tag="IW774_000970"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1153331.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            18911..19513
                     /gene="ruvA"
                     /locus_tag="IW774_000971"
     CDS             18911..19513
                     /gene="ruvA"
                     /locus_tag="IW774_000971"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1153332.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19526..20527
                     /gene="ruvB"
                     /locus_tag="IW774_000972"
     CDS             19526..20527
                     /gene="ruvB"
                     /locus_tag="IW774_000972"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1153333.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20556..21134
                     /locus_tag="IW774_000973"
     CDS             20556..21134
                     /locus_tag="IW774_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1153334.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21186..21668
                     /locus_tag="IW774_000974"
     CDS             21186..21668
                     /locus_tag="IW774_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1153335.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21785..22159
                     /locus_tag="IW774_000975"
     CDS             21785..22159
                     /locus_tag="IW774_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153336.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7331 a         4040 c         4872 g         6236 t
ORIGIN      
        1 tgatattgct tcgatatggc ctgttttact gggagcagtc gtcgtgtgtt ccatcgtcac
       61 gatggttgca gcgggaaaaa cggcagaagg tgtagaagct ttgctgggct atgtccgtaa
      121 gaaaaaagca gaaagggtat ctgtaaacga aatggaggag aatcaaattg actgagtttt
      181 ttgtgaatcc attatttggc ttaagtttga ctttaggagt gtacgtatta tctgacaaat
      241 ttttgcggaa atataaactg cctgttttga atcctctcgt attatcgatt gttgttatta
      301 tcttattttt gcatttttct gggatcagct ataaagacta taatatcggg ggcaattttt
      361 tgactatgct gattactcct gcgacagtgg cacttgccat ccctctttat aaaaacttcc
      421 gtttactgaa agagaatttt tatcctgtaa tggctgcgat tttagcagga atagtcgcaa
      481 attgcttggt tagtgtaggg ttcggttact tcttctctct tcataaagag atggtgatct
      541 cattactacc taaatctgta acgacggcta tttctgtcga tttatctcat acgatgggcg
      601 gggtgaatgc cgtgacactt gctattgttg tctctacagg tatttttggt tcgctgatcg
      661 ccactcatat tttccgccta ttcaagatcg aaagtccagt tgctcgcggt gtggcgttag
      721 gaagtacttc tcatgctatt ggtacagcaa aagcaatcga gattggtgaa atagaaggaa
      781 tcatttccgg acttgcgata tgcgtcaacg gaatattgac cgttttattg ctaccgttat
      841 tctttcagcc ttttgctggc ttgttttgat gcgaaaacca ctattttatg ctacaataac
      901 caaaacgttc gagcataaaa aaggagggaa agacgtgtat ttgtcttccg agtatcttaa
      961 acaatttgat aaagatctat attataagat tctcctccta gaaagttttg aggatcaagc
     1021 ttggcatacc gctgcacaat tagcgcaagt tgttcagctg gatgcccgca gcgtgtcgaa
     1081 atacctgaac gagctgtcta aaaactatca gcagtttagc ggtaagacac accctttgtt
     1141 tacaaaaaat caccgttctg gttataactt ttatgatacg ctcgattcaa ttgaacatga
     1201 gcgttttttg atctatctcg tgcaaagtac gctgaaattc cagttgctgc acgacatttt
     1261 ttttgaagaa ttccatacaa tgtaccaatt tgcgcaaaag cattacatta gtgaatctac
     1321 tgctcatcga aaaatcaatg aatggaaaca gcaactgcaa acttacggaa ttcggctgca
     1381 acgaggtaca tatatcgctc aaggcgaaga agaaatcatt cgactttact tgcatatgac
     1441 attctggcag ttgtttcgag ggaaaatctg gccatttgaa acgatttctc aaatggacgt
     1501 aaagaatatg gcagaacata tcatggcttt ttttaatgtt cgtctgaacg agatcaaaaa
     1561 aagacgattg gaatatatgc taggtgcctt ttttttaaga aaatcacaga agcattatgt
     1621 tgtattgaat gagaaaaaaa gacggctgat ttctgacaat ctgttgttcc agcgattttg
     1681 ccaggtcatg gagccggtat ttcctaatta ttttcaagta gaagacgagt taggcgcatt
     1741 atttcttgta ctgatgactc gtgaagaata ttatagtgat cctaagatac ggaagaaaat
     1801 ttttgatttt catcaacaag caaaaacacc gccctttaca gccctttcag aagcaaaagc
     1861 cgctctttct ctttatcagg aagagcaggg gctgccagca gaaaatctaa ccttcgaggc
     1921 agaaaactat ttattcagca gtcatttttt cgcctatctt tttcctaatg cgaaagaaac
     1981 gattgatggc aatagtagtg attttattaa tcatttagtt attgaaaaca aggaattgaa
     2041 acaatggctc gtacattttt ttgaaagtag acacaaacat cccaatcatc tggcatttaa
     2101 aaatcatgcg tttttgatgg ggcggtatct gactgtgttt aaaacattag gagcctttac
     2161 tccacagctt ccaaagatca cgatcctttt aatgacagat ttcccattat ttgaagagca
     2221 actgttagag gagggattac ggacattttt ccgtaatgag tatcagctga tttttctgcc
     2281 aacagattat cgggggagag aagttgatct tctgatttcg acaagtaaag tccaccgcaa
     2341 accatgggcg gatcttgact attttattgt gactgaagaa ctaaaattaa ttgattacat
     2401 ccaactatct caaaaatttg aaatgattca aaaacaaaaa ctattaaaac aataacaaag
     2461 aaggagaaaa aagatgacat ttgaagaaat tttaccagaa ttgaaaaaag gtgcaaagat
     2521 tattcgtaaa ggatggagtg gatttgaact ttatgttgtt ctcgtatccg atgatgaatt
     2581 tgatggctgt ccggtgacgc cgtacttatt gatcaaaaca agtgacgaag gtttttccag
     2641 cttcgctcct acagtttgtg atattttagc agatgattgg gcgatcgtag aatgaaaaac
     2701 cagtatccag atctagctaa acaggttgta tttatcacag gagctgcttc tggaattggt
     2761 gctgcgcaag cccgagcgtt tcttgaacaa gatgcttttg tatttggatt agatcaaaag
     2821 ttcggtaaaa tggaagaaat ttctttgcga tttcctgaaa cttttgccta cgcactaggt
     2881 gatgttcgac aaatgaaaga actccaacaa gcggtatcga aatgccaaaa tcattttggt
     2941 gaagttacga ttttactgaa tacagcgggg atattggatg cctacaaacc cttgatggaa
     3001 actgacgaat cgctgtggga tctgatttat gagacgaatg tgaaaagcat gtatcagctg
     3061 acaaaaatca tccttcctga tatgatccgc caaaaaagcg gaacgatcat caatatggct
     3121 tcgattgctg gcttgattgc tggtggaggt gggatcgctt atacaagcgc gaaacatgcg
     3181 attgtcggat ttaccaaaca actcgctctg gattacgcag gacagggtat ccgagtaaaa
     3241 ggaattgctc caggagccat ccaaacacca atgaatgcgg ctgattttga agggaaaggt
     3301 gagatggcag agtgggtggc aaatgaaaca ccagtcaagc gatgggcaca gccggaagaa
     3361 gtagctgagc tgacgttatt tcttgcctct cctcaagcaa gctacataca aggaacgatc
     3421 atcccaatcg acggcggatg gttgttaaaa taaccttgtg tttttttcaa gcttctgtat
     3481 gataaaacta aagagagaaa caacggttga tgataaaaaa agtcgatgac tgttcccgac
     3541 attcgatgcc gaacagccag ttcaaaaaat tctttttaat aatcgttaat attcgaaact
     3601 aagcggcttt tcacgacctc ttgatataaa cgatattcaa aagcttgcaa ctgcggtaac
     3661 aagtccagcc aaatgaaaaa tataaggagc tatttttatt tgaccattct tattacttgt
     3721 tgcagggcag ctttttcact accttctgcg acacttcagt tggaattttc ttcccatcct
     3781 ttggtcgcgg tcattaccaa gataaaatta tcttgcaaag gagaaataga acatggaaaa
     3841 aaataatacc cgttcagcca aacgctggac aacgacagat gtgactcgga tggcgctagt
     3901 cactggttta tatgtggcag tgacgctggt tttgtctgtc tttagctttg gagtgattca
     3961 gattcgtctc tcggagatgt tcaactatct gtccttgtat aacaaaagat acatcgtcgc
     4021 ggtaacagcg ggagtagctt tggctaatat tgcttctcct cttggactga tagatgttgc
     4081 agtaggaagc gtcagcacgc tgcttgtatt gcttatcaac tataaaatca ctagtcgcat
     4141 caaaaacatg aaaatcaaaa tggccgtgac agcatgcgta tttgcttttt ccatgtttac
     4201 agtagctggt cagctaacga ttttatatca actacctttt ttcttgaatt ggtgggtgat
     4261 tgctttagga gaattggcat caatgattgt cggtgggata attatttatt ggatcggtaa
     4321 aaaagtggat ttgtcaaaat aaagatctgt ttaatttaaa atagggcagc atggaagaga
     4381 ctgaatgttg agtcttcttc catgctgctt ttttgattaa tttgtatgaa gaaatcgatt
     4441 tagccgtttt ttcatcaaga taccgatata agctgctcca gttgccttga ttgcttccgg
     4501 cagtaaaaac ggcagtagtc cactggcaaa tgcagttgta aaagtaagat caccacttat
     4561 tttcagccag atcgttccgc agatcaatgc agcaagagag cccattagat tggcaaagat
     4621 tgcatataag taatgagcat ccgttctttc gatcatccaa ccagttatcc acgtttgtaa
     4681 gagaaatcca atcaaatatc cgccagttgg cccaaataaa acagctatac ctccagtcat
     4741 ccctgaaaat acaggaagac caatcaagcc aagtaaaaga taaactccaa cagatatcaa
     4801 tcctagtttt cttccgagaa aagttgctgt taatccaata gcaaatgttt gccctgtcaa
     4861 aggaaccaaa ccaaaaggaa tggtcatctg agaaaggaca gaaataatcg cggcaaattc
     4921 cgcagcaagt atcaattctt tcaatgttcg gttcataacg ccctcttttc gttaacttaa
     4981 tatttttatt tggttaacga aattgtaaaa caaagtgatt actttgtaaa tagaaaatat
     5041 aaaaaaagac cagctatcca atagatagcg gtctttttca agaaagcttt tgaaattata
     5101 agctttggtt gtagtattca acgacaagag cttcgtcgat ttctggatat agttcgtcac
     5161 gttctggtaa acgagtcaat gaaccttcca atttttcagc gtcaaagctt acgaatgctg
     5221 ggcgtccaac agttgcttct acagcttctt ttactgttac gatgttttgt gatttttcac
     5281 gaactgagat cacttgaccc acttctacgt ggtatgaagg gatatcaacg cgtttgccat
     5341 caactaaaat gtgaccatgg ttaactaatt gacgtgcttg acgacgagtc gttgcaagtc
     5401 ctaaacggta aacaacattg tctaaacgtt gttctagtaa gatcatgaag ttaacaccat
     5461 gtttaccttc tttgattttg ctagctttta cgaacaagtt acggaattga cgttcgttca
     5521 ttccgtacat gtgacgaagt ttttgttttt cagttaattg catgccgtat tctgattgtt
     5581 tgccacggct attaggtcca tgttgacctg gtgcgtaagg gcgacgtgct aattcttttc
     5641 ctgtgcctga caatgagatg cctaagcgac gagaaatttt ccatgatggt cctgtataac
     5701 gagacattaa aatttcctcc aatgaatata agtttttgga gtaaaataat ccgttgaaaa
     5761 attcataatc atatagtttg ttcttcaatc ttcacctttg cagccgcggt tacacaatcg
     5821 aacccgagac ggggcaacaa actagggatg agttattatt tttctgctgc attattttac
     5881 acaaattaca gtatatgata gatcatccac gaatgtcaag ataattagta cataaaaacg
     5941 ctttacaaac ctaggctgtc tttataaaat gagagggtat gtgcaaatgg aaaaaagcga
     6001 aggaagttta aggagaaaat caatgaatcc ggtatcacca ttatttgaga aaatcgatgc
     6061 ttcaattgaa aagaaggcga atctttttga aaacagcttt tcacgttacg ctgttcgagc
     6121 ggttctcgcc tgtttgtttt tgactttagg aacggctgtg gcatttgcga tcgcgatgaa
     6181 gggggaagat atcgcgcatg gtctaggaaa gattttatac gcatttatgt tcagctgggg
     6241 tctggtcatg attctataca tgaacgccga attaggaaca tcgaatatgc tttatatgac
     6301 cgtgggtgtt tttcggcaga gacttgcttt aagtaaggca gccaagattc tatttgcctg
     6361 tatttttttc aatatgattg gcggtatatt atgtggattt ctaatttcta tgactacgcc
     6421 atttcaagat ttaacgttgg acaactttat gttagaatca atagcagtaa aactagcaaa
     6481 accttcagca acgattttgg tggaagggat gtttgcgaat atcgttgtca ataccgctgt
     6541 actgatcagc atgcggatga aagaggatgt cggaaaagtg atcacagtcg tattcatcat
     6601 tttcattttt gcttttttag gatatgaaca cgtgatagcg aattttcctg cctttacgct
     6661 tacttacttt gcttctcacg gacagatgga tggaatgaca gtcagcaatg tgttacataa
     6721 tttgtttttt gctttggcag gaaattatat tggtggcggt ttagtaatgg gactgggata
     6781 tgcttggctg aatcagtcaa aatcatccta tgtggactaa aaagtcgttc attaaagggg
     6841 aagtgccatg gaagttttag tttttccaaa aaatgaggaa aaagcgttac aaatggaaaa
     6901 gtacatgaaa aatctttttt cctttgctgg agtacctaag ccggaacgcg ctcttttaca
     6961 acaaccgtta ctggatgaga gcaaaaattt atctatttct gaagttttag cttttgttca
     7021 ttattattat gaacaaatcg aaagagagta tcagtatgta gcgatcgatt tagtccagaa
     7081 aaacatcaaa cggcttacat ttgaagactt agtcgtattg ttgccactag ttcagaaaaa
     7141 agaatggtgg gacagcatcg actcttggag aaaagtatac agcacatggt gtaaagccta
     7201 cccagagcag cgtgaagaag tctttcaatt gtttgaaaaa aaggaagatt tttggctacg
     7261 tcgaatagca atcacgttgc aattaggtta taaacagcaa acagacacag gactattgat
     7321 ccgagcaata gacgaagatc gtcaaacaaa ggaattcttt atccaaaaag caattggttg
     7381 ggcattacgc gattatagta agaccgatgc cgattgggta aaaagacagc tgacaacaga
     7441 tttgagtaag ttagctgttc gagaggcaag taagtattta taagaaagcg agacgacaag
     7501 agatgagacg caaattattt gcttttgata ttgatggaac gttgttagat tcaaacaaac
     7561 aagctttaga tagcacaaga gaagcattgg aaaaattacg caaagccggt catctggtca
     7621 cgattgctac agggcggagc cgatttcatg cgcaagagat cattcgcgat cttgatttta
     7681 cgaactacat tttatgtaat ggagcggccg gttttttaga ccatgaacaa gtatataaga
     7741 atttactaga tcgcgaccag ttggatgcgt tcgttcaaga agcgcatcat gagcagatcg
     7801 atacagcttt tgtcagctta gacaatatca agcgttcttc ttccaatgat gtaacggtga
     7861 tggaagaagc gatgcattct ttcggtgctg tcttgcctga attagatcag ttttttgcag
     7921 aggaacaaga aatataccaa gcgttagcat ttttcgatca aacatatgat gggaagttct
     7981 cttcatatga taaaattcga tttgtccgct ggcatgagaa cagtgtagat gtcgtaccta
     8041 aaaatggttc taaagcagcg acaattctgc acttagcaga acaagtaggc atccctcatg
     8101 aagacatcat cagctttggc gatggacaaa acgaccgtga aatgttgcgc atgtcaggaa
     8161 ttgggatagc tatggggaat gctgtgccag aagtacaagc agaagctaag atggtcactg
     8221 atacaaacga tcaggatgga atttggaagg cattgaaaga gttaagcgtg atttaaaaga
     8281 ggttgtgaaa aagctgttct gcatcaagta ataagcccag ccaaacaaaa atagctcctc
     8341 ctatttttcg tttttctggg cttattaccg cagatgcaag ctttcgaaca ccgtttaagg
     8401 aagaggtcgt gacagaagtg gtcagtttca agaaataaga agggatttac gaaaattgct
     8461 tctcaaattt ttgtaaaatt acggcttatt tctgaagaag tagatgaaac aacgtttatt
     8521 cgggaaatgg gactgtgaca agatgtttgt catagtccca tttttttatt ttatttttca
     8581 aaactaagaa ataccaggaa agagttcctt ttgtttgacc ttttctttct tattgaaaac
     8641 aactataata tagagtgatg ttaaaagaga agaggaattt agtgtgcggg tattgtttat
     8701 tggtgatgta gtaggttcaa tgggacgaga gatgattact gaatatcttc ctagattgaa
     8761 gaagaagtac cgtccgcaag taacgattgt aaatggtgag aatgccgctt ctggacgtgg
     8821 aatcacagaa aaaatctata aaaaattttt gcaagacggc gtagatgtcg tgacgatggg
     8881 gaatcatacg tgggacaacc gcggaatctt tgagtttgta aatgaagcta aaaagatggt
     8941 tcgtccggca aattttccgg aagggacgcc tggacaaggg atggtctttg tcaaagtgaa
     9001 ccaagtcgaa ctggcagtga tcaacatgca ggctcgttct tttatggtgg acttggatga
     9061 tccttttcgt aaaatgaatg agctggtaga agaagccaga aaacgtacgc cgatcatttt
     9121 tgtagatttt catggagaaa caacaagtga aaaacaagca atgggctggt ttttagatgg
     9181 gaaagtttca gcagtggtcg gtacacatac acacgttcag acaaatgatg cgcgaatttt
     9241 gccaagagga acagcgtatc tgacagacgt tgggatgaca ggtccctatg atggtatttt
     9301 gggtatgcgc agagaacctg taatcgaaaa gtttctgact gcattaccaa aacgttttga
     9361 agttgtggaa caaggccgat cgattttaag cggctgtatc ttggaattgg atgatacgac
     9421 aggtcatgca aaagaaatcc agctgattca gatcaacgat gatcgaccgt tcatggagta
     9481 gaaagagagg cagttttttg cagcaggagc cggaaattca aaaagaagta aggaaactga
     9541 caaaactttt aagagagaat gaaacgatca ttcgctacaa agaacttgaa gaaaagatcc
     9601 agcaaaacca gtatttagct gagttgagag aaaaaatcaa acaagcgcaa aaagatgctg
     9661 ttcattttgc ccactatgac aaaccagcag cagaaaaaga agcaatcaaa caagcggatc
     9721 aattcatgca agaatttgat cagcatccgc ttgttgtagc ttatagaaaa cagctgttgg
     9781 aagcagatga tctgcttcat catttaacaa ctatgataca agaagaaatc aatggacaaa
     9841 tagaggagga aaaacatgcc tcaaaaaact aaaaatactc ctatgatgga acaatatcta
     9901 agcatcaagg cccaatacca agatgctttt ttgttctatc gtttaggaga cttttatgaa
     9961 ctattttacg aagatgcgat caaagcgtca cagcttttgg aattaacgtt gacgagcaga
    10021 aatcgaaatg cagaagaacc tattcctatg tgcggtgtac ctcaccatgc agcacaagga
    10081 tatatcgatg ccttgattga aaaaggctat aaagtagcga tttgcgagca ggtagaagat
    10141 ccaaagacga ctaaagggat ggtcaagcgc gaagtgatcc agctagtcac tcctggtaca
    10201 gtcatggaca gcaaaggatt gtcggcaaaa gacaacaatt ttttaacagc tgtagttagt
    10261 caaggaaatg aatttggctt tgcttatgcc gatttaagta cgggagaatt gaagacggct
    10321 cggttgcatg atgaagaagc tgttttaaat gaagcctctg cattgcagac aaaagaactc
    10381 gtattaggca gtgagatcac tgataccctt cgtgaacagc taagcgggcg tttagggcta
    10441 gtcttttctc aacaaaatga gatggaagaa aacgcggaat ttagcttttt gacaagcgaa
    10501 ctagtcgatc cgctggaaaa agaagcaacc ggcaaactat tgacttattt atcggtgaca
    10561 caaaagcgga gtttagctca tatccaaaaa gcagtagaat accaaccaga tcattttttg
    10621 aaaatggatt attattcaaa attcaaccta gaattaagcc aatctattcg gactgggaaa
    10681 aagcacggca ctcttctatg gttgttagat gaaacaaaaa cagcaatggg tggccgttta
    10741 ctaaaacaat ggctagaccg tccgcttatc caggcaaaac aaattaaagc aagacaagaa
    10801 atggtcgctt ctttgctaga cagctatttt gaacgtatcg atttgcagtc agcattaacg
    10861 aaagtctatg atctggaacg cctagcaggt cgagtagctt tcggtaatgt caatgggcga
    10921 gacttgatcc agctgaaaac ttctttagaa caagtgccaa tgatcagagg gttgattgaa
    10981 ggaatcgatc aaggtcaatg gcaaagtctg ctttcagaaa tgcagccgat ggaccatctc
    11041 gtccaattga tcgaccaagc tatccaagat gatccgccgt tacaaataac ggaaggaaac
    11101 atcatcaaag atggattcaa tgagcagctc gatatgtacc gttctgctat gagaaacgga
    11161 aagaaatggt tggcagaact tgaagcaaaa gagcgtcaag aaacaggaat caagaactta
    11221 aaaatcggat ttaaccgcgt tttcggttat tatatagaaa ttacaaaagc aaatcttgga
    11281 aatgcagaac tggagaagta tgaacgaaaa caaactttag caaatgcaga gcgtttcatc
    11341 acaccagaac taaaagaatt ggagacacaa atcctcgaag cagaagaaaa atctgttgat
    11401 ttagaatatc agttgttttt agctgtgaga gaagaagtca aaaaggctat ccagccgcta
    11461 caagtacttg caaaagcaat cagtgcagta gatgtgctac aaagttttgc aacgatcagc
    11521 gaacgctatc agtatgttcg gccagaactt gtcagtgaca agcatcagct tctgatcaaa
    11581 gacggacgac atcctgtagt agaaaaagta ttaggacatc aggaatatat ccctaacagc
    11641 gtagaaatgg cagaagacga aatgatcttg ctgattacag gaccgaatat gtctggtaaa
    11701 agtacgtata tgcgacagtt ggcattaact gttctgatgg cgcaaatggg ctgtttcgtt
    11761 ccggcaaaac aagcagtttt acctattttt gatcggattt ttacccgaat tggtgcaagt
    11821 gacgacttaa ttgctggaca aagtactttt atggtagaga tgatggaagc caatcaagcc
    11881 ctgcgttatg ctacgccaaa cagtttgatt ttgtttgacg aactagggcg aggaacagca
    11941 acctatgacg gaatggcgtt ggcccaagca atcatcgaat atatccatcg tcatgtacaa
    12001 gcaaaaacac tcttttctac tcattatcat gaactgacgg tacttgagaa agaacttccg
    12061 cagcttaaaa atgtccatgt tggagccgtt gaaaaagacg gagaagttgt cttcttgcat
    12121 aaaatgatgg atggacctgc ggataaaagc tacggcatac atgtggcaaa gatcgcagga
    12181 ttgccttcca atctactaga acgagcagca gatatcctgc atacgctaga aaatggcgat
    12241 aacggacacc atactgttcc agctgaagta gaagagaaaa ctaaaacagc caaacaggag
    12301 caaataaaac cacctgcaga aaaagtaaat caagaccata tacaagaccg tgtaaaagaa
    12361 gaaaaccaac agctttcttt attcagtgaa ttatccgaaa atgaaacaga agtcattgaa
    12421 tcactgaagc aaataaattt actagaaatg acgccaatgg atgcactaaa cacattatat
    12481 gaattacaaa aacaactgta ggaagaaggc ggatatcgat ggccaaaatc caagaattat
    12541 ctgaacgttt agctaatcag atcgctgcgg gagaagtagt ggagcgacca gcttccgttg
    12601 tgaaagaatt agtggaaaat gcaattgacg ccggcagtac tcagattgat atcttagttg
    12661 aagaagcggg actgcggacg atccaagtga ttgataacgg agaaggcatt ttggcagacg
    12721 acgtagagaa tgccttcaag cggcatgcga caagtaagat ccataatcgc gacgatcttt
    12781 ttcgcatacg aacgctgggc tttcgagggg aagctttgcc aagtattgcc tctgtttctg
    12841 aaatgactct ggaaacggcg aatcaggaag aaaaacaagg cacctatctg tcgctgaaag
    12901 gcggagaagt tattgagcat cgaccagcac cactgcgtca aggaacgaag attaccgtca
    12961 gcaatctgtt tttcaatacg ccagcccgct tgaaatacgt gaaaacactg cacacagaat
    13021 tagcgaacat cggggacatc gtcaatcgac tggcattgag tcatccatcg attgcttttc
    13081 gtttagtaca tgatggaaac aaaatgctgg caactgctgg aaatggcgat ctgaaacaaa
    13141 caattgcggg aatctacgga ttatctaccg caaaaaaaat gctgaaaatc gaaggaaaag
    13201 atctagattt tgagttattt ggctatgttt cattgcctga ggtgactcgt gccagtagga
    13261 attacttatc gacgatcatc aatggacgtt ttatcaagaa cttttccttg aataaagcaa
    13321 tcgttgcagg ttatgggtca aaattaatgg tcggtcgttt ccctattgct gtattggaaa
    13381 tcaaaatgga cccgctttta gtagatgtaa atgttcatcc gacgaagcag gaagtccggc
    13441 tgtcgaagga aaaagaacta acagagctaa ttagtaacgc cattcaaaaa gcattgtctc
    13501 aagaaaatct gattccaagt gcagcggata atctacggtt taagaaaaaa gtacctgatc
    13561 agcctaaaac agaacaaatc gaaatcgatt tgtcctacgc ttttgaagaa gaaaaaccta
    13621 aaaagccaag tagtttgaat tttgatcgag agacaggtac ctttttcgta gaagaactaa
    13681 agaacacatt tgttgacaga attgttgata agtctgtgga aaatgttgaa aactattctg
    13741 gtttttctga agaaaacacc acttatccag aaaaaacagc gaaaacggtg gataagtttg
    13801 gtgaaaatgt tgataacttt gtagaaaaaa cagtggaaaa atctgaaaag aaagaatttc
    13861 ccattgtgga taacatggag aaaacaaatg aagaacaagc agataaatcc gtagaagagg
    13921 atgaaaagat ttacgaggaa gaattgacac tccatcctga atttgatttc actcaaggaa
    13981 acgacaaaac attagaacaa gccatcaaga aattagaaac ggaaaaacca gtacaacgtt
    14041 ttcctcagtt ggaatatttt ggacagatgc acggaacgta tctttttgcc caaagtagcg
    14101 atgggttgta catcattgat cagcatgctg cccaagagcg cattaagtat gaatatttcc
    14161 gggaaaaaat tggggaagta tctgatgatc tgcaagaatt gcttgttcca tttgtgttag
    14221 actatccaaa cagtgatgct ttgaaattaa aagagcaaaa acacttgcta gaagatgcag
    14281 ggatttattt agaagaattt ggtcaaaaca gctttattgt acgggcccat cctacttggt
    14341 atcccaatgg agaagaagaa tcgattattc gggagatgat cgatatgctg ctgacaactg
    14401 gaagtgtgag tgtaaaaaaa ttccgtgaag caacagcaat catgatgagt tgcaaacgat
    14461 cgattaaagc caaccattat ttgaatgaag cacaagcacg tgtgctgctg aaggatctgg
    14521 cacgatgcga aaatcctttc aattgtcccc atggccgtcc agtattgatt cactttacca
    14581 attcggatat ggaaaggatg ttcaaacgaa tccaagaccc gcattaagtc aaagagaaga
    14641 gggaaaacat gaagattgta ttagcatccc aatcaccaag aagaaaagag ttattggctc
    14701 atatcgtttc agaattcgag attgaacctg ctgatatcga tgaaacgcca tttccagacg
    14761 aacagcccgt cgattatgtt cgtagaatgg ctgaagaaaa agcacgaact gtctgggaac
    14821 aagatgaaaa agaagaccat ttagtgattg ccagtgacac gactgttgtt ctcaatcaag
    14881 agatcatggg taaaccagaa gatctggctg aggctgaatt catgctgaaa aaactaagcg
    14941 gggaaacaca tactgtatat acagctgttg ttttaaaaac aaaggaacga gaagaacgga
    15001 tactagcaga agcacatgtc actttttatc ctttgacaga cgaagagatc aaacgctact
    15061 tggagacggg ggattatgcg gataaggcag gggcatacgg catccaaaat gctgcgagtg
    15121 tattcgtcaa agaaatttct ggagattatt acagtatcgt aggatttccg attggtgctg
    15181 tcaatcaggc actgaaaaca ttcatttaaa caaaaaagcg gactatctga ttttcgattg
    15241 aaaatccatg atagcccgtt ttttttattg aaaagattac ttatctactc tgactaagat
    15301 tttacttcag ctttaccgac tgcttcgccc gggcgaacaa gtcctgattg gtcaagagaa
    15361 agtcctgcaa ctttttcatt attggtcaaa gcaacaataa tgtcagtttt cttgccagct
    15421 tgttctattt ctggcaaatc catttcagca atcagggtct ctggtgtgac aaggtatcct
    15481 tctttcacga atacattgaa aggaacaccc tttagttcaa cagtatctaa tcccatgtga
    15541 acaagtactt ccagcccctc attagaaaga ataccaatgg catgttttgt ttcaaagaca
    15601 ctcgtcactt taccgtttac tggtgcatag actttgccgt ttgacggttc gacagcataa
    15661 ccgtcaccca tcattttttg tgagaaaact gggtcattta cttcagttat ttccttgatg
    15721 gtccctgcag cgactgcgaa taatgtttct tttgttgcaa ctgccgtacc agttgctgaa
    15781 acaggttcga aagactgtgg cgttgctgtt gcgccatttg ctgtttgaag ttgtacatca
    15841 gcagcatttt caggaacagc aggatcaatc ttattcaaaa ttccctgacg tctaaagatg
    15901 atcgtcaagc caaatggaat gatgacagca atgatcattg cgatgatgaa aggtacccat
    15961 gtttctgctt ggatagctag tattcctggc aaaccgccta ctccgatcgc gttagctcga
    16021 acacccatca agttagcaaa catgccagct aatccagagc cgaccattgc agcgacgaaa
    16081 ggatagacgt atttcaaatt gattccgaac atagcaggtt cagttacgcc taagtaacaa
    16141 gaaatcattg ctggaatcga gatttgttct tcttttttat tcccacgatg taagaagatg
    16201 atggctaaga ctgcagagcc ttgggcgata tttgataaag caatcatcgg ccataaattc
    16261 gttgatccga aatctgcaat cagctgcata tcgatggcat tggtcatatg gtggagacca
    16321 gtaatcacta aaggagcata caggaatccg aatacggcac taaacaacca gctgatagaa
    16381 gacgtcaatc cagtatttac gatatttgag atccagcttc cgactgtcca gccgatagga
    16441 cctaaaataa cgtgtgcagc taaaacagtt ggtaacaatg aaaacaatgg aacaaaaatc
    16501 atcgagatcg actgagggat atatttgcgg aagaagattt ctagatacgc taacataaac
    16561 cctgccagca tggctggaat aacttgcgct tgataaccaa tcatctggac ttgagcaaaa
    16621 ccaaaatccc aaaatggaat atctgctgcg gctgtggaag ctacactata agcatttaat
    16681 aattgcggag aaactaacgt aattcctaaa acgattccta atatttgtgt agtacccatc
    16741 ttttttgcaa tactccaagt gatacctact ggaagaaaat ggaaaattgc ttcacccggt
    16801 aaccacaaaa aggcattgac accattccaa aacttcgagt gttcaacgat tgttccacca
    16861 agactttcaa actggatacc ttcaagtaca tttctgaatc caaggatcaa tccaccgaca
    16921 atgattgcag gaatcagtgg tgtaaagatt tctgctaaaa cggcaatagc acgttgaacg
    16981 ggatgaaggt tttgttttgc agctgctttt ccttgttctt tggaaacccc ttcaactcca
    17041 gatactgcaa cgaagtcgtt ataaaaagta gaaacatcgt ttcctatgat gacttggaac
    17101 tgtcctgcat tcgtgaacat cccttttacg ctaggtatat cttcgatcgc tttttcgtcc
    17161 gcttttcctg gatcattcag gacaaaacgc atacgagtcg cacaatgact aactgcagca
    17221 atattttctt ttccgccgat atcatgtaac agcttctcgg catctgcttg atattttgcc
    17281 atatcaataa acccctttct cttaacttgt ctatgcaagt tgtttcttca tttactatac
    17341 tttttttcgg agagaattgc aagcgtttta ttaaaaagta cgagagaaaa tgatatcgtt
    17401 ttaaaaagct taaaataagc ttttcttaaa aataggaaca ttgaaataca aaaagagctg
    17461 tagtatagtg aagaaatgat ggaagaacga gtgagggaaa aaacatgaat aagttcaatg
    17521 aaattttttt agatttggaa cagaaaattt tagcgaaaga atatcctccg cattcgcttt
    17581 tacccagtga gaaccaattg atcaaaattt atggtgtttc acgagaaaca attagaaaag
    17641 cgctgaatct tttgactacg gaaggttata ttcaaaaaaa acaaggaaaa ggttcgatcg
    17701 tactggatcg gaatcgcttc gactttccta tttctggatt gactagctat aaagagctac
    17761 aggagaccca acgtattcca agtgaaacga tcatccatac gttagaagaa acagaagtga
    17821 caaaagcatt gaacgagatc actggctggg agatcggtgc gcctgtttgg catttgatac
    17881 gcgaaagaaa aatcaatggc gaagtagtta tattagacac cgattatcta ttaaaggaaa
    17941 tcgttcctat tttaactcca gcacaagcac aaggatcgat ctatgaatat tttgaaaatg
    18001 agctttcttt gacgattgct tacgcgcaaa aggagatcac agtagaagaa gtaacggaaa
    18061 tcatgaaaac agcgatggat cttcacgatg acacttatgc agtagtagtc agaagcctcg
    18121 ttcatctgga agatacccgc tgtttcgagt attctgagtc ggttcaccga ttagataaat
    18181 tccgtttcgt tgagtttgcc cgacgccgaa aagtgtaaat agttcaaaag aaagcaaatt
    18241 gttggcatct gtccactttg gcgctatgat aggatcacaa agggggcatt tttaatggat
    18301 aaaccaataa gaccagaaaa agaagaactg aaagaaaagt tatcacccat ggcttatgca
    18361 gtcacacaag aaaatgcaac agaacgacca tttagcggga aatatgatga tttttacgaa
    18421 aaggggatct atgtagatat cgtcagcggt gaaccgttat tcagctctgc agaaaaatat
    18481 gatgcaggtt gtggctggcc atcattcagc aaaccaataa cgaaaagaca agtgcgcgaa
    18541 aaagccgatt tttctcatgg catgcatcga atagaagttc gaagcaaaga agcagattct
    18601 catctcggac atgtgtttac cgatggacca gtagatcaag gtggtctacg ttactgcatc
    18661 aattctgcct cactgaaatt cattccatat gaaaagatgg atgaattagg ttatggggaa
    18721 ttcaaatcac ttgtagaata atggaaatac acaaagagcg gtacaatacg gggatttact
    18781 cgatcgtccg ctcttttttt attcaagcga ttgctatttg aaagaaacgc tccctagact
    18841 ccttttttca caaggtgaaa tgtgctacaa taaagggaac gtatgtgtgc aaaagaaaga
    18901 ggactagaat atgtatgaat atctgacagg tgtggttacc tttatcaacc cctattacat
    18961 agtagtagaa accaatggta tcggctacca gatcgcacta ggaaatcctt accgctacag
    19021 tagcaaacta aatcaaaaaa taaaaattta tgtccatcag gtaatacgtg aagatgcgca
    19081 tcttctgtat ggatttgatt cgctagaaga aaagcaactg tttttgcgat tagtaagtgt
    19141 ttcaggaatc ggtccgaaaa gtgcccttgc aatcatggca agcgaagacc actccggttt
    19201 gattcaagca gtagaatcca gtgatgtcac ttatttgacg aaatttccag gagtagggaa
    19261 aaagacagca caacaaatga tcttggatct aaaaggaaaa ttcggtgagc tgagtatcga
    19321 tacacctttt agtctatttg atgaagcaaa cacaaaagac gctacagctc tatctgaagc
    19381 aatggaagct ctctcggctc ttggttacag tgatcgagaa atcaaacgtg tagaaaaaca
    19441 attaaaagaa gtagataatc agacaacgga tgagtatcta cgccaagcac tcaagctaat
    19501 gatgaaaaaa taaggaggag accaaatgac agatgaatac ttgttatcac cagaatctgg
    19561 cgaaaatgaa ctgagtttgg aaaaatctct tcgaccacag ctgctttctc aatatatcgg
    19621 acagagaaaa gtcaaacaag aacttcagat ttacatcgaa gctgccagaa atcgggaaga
    19681 acctttagat catgttctgt tgtacgggcc tcctggatta ggaaaaacga cgatggccat
    19741 ggtcattgct aatgagatga ccgtaaatat tcgtacgacg agtggccctg ctatcgaacg
    19801 tccgggtgac ttagtagcaa tcttgaatga actggaagca ggggatgtcc tgtttatcga
    19861 tgagatccat cgattaccta gagtggtaga agagatgctt tattcagcta tggaagattt
    19921 ctatgtagat atcatggtag gacaagggcc aaccgcacat ccggttcatt tccctcttcc
    19981 tccctttact ttgattggcg cgacgacacg ggcaggtatg ctttctgcac ctttacgaga
    20041 tcgatttggc atcatttctc atatggaata ttatgacgaa caggatctgc aggagatcgt
    20101 tttacgttct tcggatattt ttcaaacaga gatcattgaa gaaggggcat tggaaattgc
    20161 tcgccgatca cgaggaacgc ctagaatcgc taatcgcttg ctcaaacggg ttcgtgattt
    20221 tgcgcaagtc caaggagacg ggaaaatcga tcgtcctata gcggatcagg cacttacttt
    20281 gctacaggtc gatcaggctg gattggatta tgttgaccag aaattgttgc gaacaatgat
    20341 cgagttatat ggcggcggac cagtagggtt aagtacgctt tcagtaaaca taggagaaga
    20401 gcgtgagacg gtagaagata tgtatgaacc gtatctgatc caaaaaggat ttttaaagcg
    20461 tacacctaga gggcgaattg ctactgctta tgcctatgaa cattttggtt acgactatca
    20521 tatatgattt ttgcttttag attggaggaa aatgaatgag tgatacaaca cgtacgaaac
    20581 aattgatcaa aaacgtattg attgatttgt gccaaacgca atcttttcga aaaatcagcg
    20641 tccaaaaaat cgcaaacgaa gcaaaaatca atcgacaaac attttattat cattttacag
    20701 ataaatacga tttgcttcgt tgggtctatt gtgaggatgc attgcattat ttagatccac
    20761 aatgtctttc acttgataat tgggaagagc aagcattgaa aatgctgaaa gcaattgctg
    20821 agaagaaaga attttatgca aatactgtgc tttctgaccc agaaatttta caaaaagagt
    20881 tttctcaact gatccaaaaa tcatttatca agatttttga acaaatggat gaagaaagac
    20941 aactttctgc aagagacaaa ctattttatg ctcgtttttt ttcttacggt tgcagtggtg
    21001 tattagtcaa ttggatcacg aatgaatatg aagaaacacc gatggaaatc gctatgcagc
    21061 ttttccgttt agccaaggat acggaattat attcttatcg tctttatgca aaagaagagt
    21121 tggaagaaca gtaatagaaa aattttttgt tggaaaagca ataatcgcta aaaggagatg
    21181 gggaaatgac aaaaatttta tttgtttgtt tgggaaatat ttgccgttca ccgatggcag
    21241 aaggcttgct gagaaaacga atagcattag aaggaaggga agcagaattt tttgtggatt
    21301 ctgctgctac aagcacgtat gaagttggaa agacaccgca ccctggaacg aaaaaaatat
    21361 taaaccaaga aaaagtagat atgacgaata tggttgctcg gcaaatcaca ccccacgatt
    21421 ttgaaacttt tgattggatc attggcatgg atcaagaaaa tgtagaagaa ctgaaacgaa
    21481 gagcaccacg ctcagcgcag gggaaaatcc atttgttttt aagtagcatt cctggaaaag
    21541 aaaaagaaaa tgttcccgac ccatactata ccaataactt tgatgagaca tatcacttga
    21601 tcacagaagg cctgaatcat tggtggaata tttggattga tgaaaataaa ataacaaaca
    21661 atttgtaaag ttaacgcttt ctttttaagg cgatgttaga gcatttcctt gtttttttgt
    21721 aaaatgttag gtaaaataaa attaattaag taacactttc ttagctaaag gaggagtgca
    21781 ttcgatgaac gaagagacag tatcgccaat gaagaagctt acgaagttag ttactcatac
    21841 ttcgtatcaa ctttttgatt cttttgtaaa tgacactgaa gaacatgcta agaatgtacg
    21901 attgcaagga aagtttattc ttgagctgca aaaagctgct actgaacata gtctagttat
    21961 cttacaagta agagcaaatc gtttgagtag caagtatgaa acgatctcag gctgggtggc
    22021 gacaaaaacg gtcgaaaaag accgagtgat gatccgccta caaaacgaag agcagcagat
    22081 ccggatggtt cctgtagaac aaattgaaaa aataacaatc ctttcaccag caggggaaca
    22141 ggaacggtta tcaaaataat ttataccaga tagaaaagca cgtatgagct tgtaccatca
    22201 atcattacta atcaaaaata accgaatgct ttaagctgat atcagtaaaa cggaacgttt
    22261 taggatgagt atcatagcat gacagcattc ggttgattag ttgaatcgat tggatggtac
    22321 aagttttttt gataaaaaaa ggtcctgcta gccggggagc tagcaggata aggagttaaa
    22381 aatgaaaaag tgttgttagg gttgtttgtt ggtatgctta tataatagtc attagttgtg
    22441 aatattttgt gacgaaagtt ttcgaaagtt gttaagttt
//
LOCUS       DACEOE010000030        21850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEOE010000030 DACEOE010000000
VERSION     DACEOE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.031"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IW774_000976"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IW774_000976"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1153337.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IW774_000977"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IW774_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1153338.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IW774_000978"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IW774_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1153339.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IW774_000979"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IW774_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1153340.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IW774_000980"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IW774_000980"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1153341.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IW774_000981"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IW774_000981"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1153342.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IW774_000982"
     CDS             complement(8053..8523)
                     /locus_tag="IW774_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1153343.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IW774_000983"
     CDS             complement(8569..8994)
                     /locus_tag="IW774_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1153344.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IW774_000984"
     CDS             complement(9061..9330)
                     /locus_tag="IW774_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1153345.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IW774_000985"
     CDS             complement(9341..10936)
                     /locus_tag="IW774_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1153346.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IW774_000986"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IW774_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1153347.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IW774_000987"
     CDS             complement(14489..15049)
                     /locus_tag="IW774_000987"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1153348.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IW774_000988"
     CDS             15402..16376
                     /locus_tag="IW774_000988"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1153349.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IW774_000989"
     CDS             16546..17946
                     /locus_tag="IW774_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1153350.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IW774_000990"
     CDS             complement(18024..18746)
                     /locus_tag="IW774_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1153351.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IW774_000991"
     CDS             complement(18825..19331)
                     /locus_tag="IW774_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1153352.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IW774_000992"
     CDS             19454..21253
                     /locus_tag="IW774_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1153353.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..>21850)
                     /locus_tag="IW774_000993"
     CDS             complement(21321..>21850)
                     /locus_tag="IW774_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ1153354.1"
                     /translation="VPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPE
                     QPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGR
                     DIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVK
                     VGSVQWFAPDFAVSM"
BASE COUNT         6053 a         4792 c         3969 g         7036 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg
//
LOCUS       DACEOE010000031        21264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEOE010000031 DACEOE010000000
VERSION     DACEOE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.033"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(104..1336)
                     /locus_tag="IW774_000994"
     CDS             complement(104..1336)
                     /locus_tag="IW774_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1153355.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="IW774_000995"
     CDS             1591..2160
                     /locus_tag="IW774_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1153356.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="IW774_000996"
     CDS             complement(2338..2778)
                     /locus_tag="IW774_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1153357.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="IW774_000997"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="IW774_000997"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1153358.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="IW774_000998"
     CDS             3732..4655
                     /locus_tag="IW774_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1153359.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="IW774_000999"
     CDS             4731..5870
                     /locus_tag="IW774_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1153360.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="IW774_001000"
     CDS             5863..6663
                     /locus_tag="IW774_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1153361.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="IW774_001001"
     CDS             6663..7490
                     /locus_tag="IW774_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1153362.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLADDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="IW774_001002"
     CDS             7468..8202
                     /locus_tag="IW774_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1153363.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="IW774_001003"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="IW774_001003"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1153364.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="IW774_001004"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="IW774_001004"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1153365.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="IW774_001005"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="IW774_001005"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1153366.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="IW774_001006"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="IW774_001006"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1153367.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="IW774_001007"
     CDS             11787..13820
                     /locus_tag="IW774_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153368.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="IW774_001008"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="IW774_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="IW774_001009"
     CDS             15353..16159
                     /locus_tag="IW774_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1153369.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="IW774_001010"
     CDS             16171..17391
                     /locus_tag="IW774_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153370.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="IW774_001011"
     CDS             17381..18967
                     /locus_tag="IW774_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1153371.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="IW774_001012"
     CDS             19006..21144
                     /locus_tag="IW774_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1153372.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6683 a         3613 c         4471 g         6497 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagccgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagt
//
LOCUS       DACEOE010000032        20799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEOE010000032 DACEOE010000000
VERSION     DACEOE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.034"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW774_001013"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW774_001013"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1153373.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IW774_001014"
     CDS             complement(2260..2511)
                     /locus_tag="IW774_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1153374.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IW774_001015"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IW774_001015"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1153375.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IW774_001016"
     CDS             3461..3679
                     /locus_tag="IW774_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153376.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IW774_001017"
     CDS             complement(3968..4480)
                     /locus_tag="IW774_001017"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1153377.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IW774_001018"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IW774_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1153378.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IW774_001019"
     CDS             complement(6933..7367)
                     /locus_tag="IW774_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1153379.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IW774_001020"
     CDS             complement(7377..8165)
                     /locus_tag="IW774_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1153380.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IW774_001021"
     CDS             complement(8162..9103)
                     /locus_tag="IW774_001021"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1153381.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IW774_001022"
     CDS             complement(9280..9867)
                     /locus_tag="IW774_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1153382.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IW774_001023"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IW774_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1153383.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IW774_001024"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IW774_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1153384.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IW774_001025"
     CDS             complement(12784..13719)
                     /locus_tag="IW774_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1153385.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..14864
                     /locus_tag="IW774_001026"
     CDS             13905..14864
                     /locus_tag="IW774_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ1153386.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14950..16044)
                     /locus_tag="IW774_001027"
     CDS             complement(14950..16044)
                     /locus_tag="IW774_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1153387.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16233..16994
                     /locus_tag="IW774_001028"
     CDS             16233..16994
                     /locus_tag="IW774_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1153388.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="IW774_001029"
     CDS             complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="IW774_001029"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1153389.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17769..18524
                     /gene="fabI"
                     /locus_tag="IW774_001030"
     CDS             17769..18524
                     /gene="fabI"
                     /locus_tag="IW774_001030"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1153390.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18634..19647)
                     /locus_tag="IW774_001031"
     CDS             complement(18634..19647)
                     /locus_tag="IW774_001031"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1153391.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19812..20732
                     /locus_tag="IW774_001032"
     CDS             19812..20732
                     /locus_tag="IW774_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1153392.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         6001 a         4467 c         3685 g         6646 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggac gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga
    14221 gaacacttta tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag
    14281 gtaaaagaaa acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa
    14341 tccatgatcg acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg
    14401 atacgatcgt tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta
    14461 aagtcattat cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta
    14521 atcaatggtt ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa
    14581 aagaattttc taactggaaa gccattagta accaacatga tattgatata tattttgcgg
    14641 accctggaac accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta
    14701 atggactgcc gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca
    14761 gcaatcaacg taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat
    14821 ttttgagcta tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata
    14881 aaaaaacaaa ataaaaaagc agaaataact ggctatttct agcggattat ttctgcaata
    14941 actaattatc tattttttat tttcaattaa tttaaagctt gaccatggtt tcaaaaagtc
    15001 aaggaggaat aattcgtcat cggagattct tcctacaaca ttgacacgtc cgtcgttttt
    15061 catatccttc aaggcgattt gtgtttctcc tttgtattga ccgtaccctt cattgtcaat
    15121 cagtacatcc ccatgatgca tatctcgcgt attatgaggc gggaaatctt tatctttgta
    15181 gtatacacga gtcatcgttg aacgaaggat atattctgag cgatcccctc gataactatg
    15241 aaggttatca aacaagcaga ttcgttcgtt ttctgtaata ccttcttccg tatctacttt
    15301 caatgttgga taatccgcgt taaatgcttc tgccatttct ttcaattctt cttcacttgc
    15361 gtacgcattt ccaatagaaa tatcatcaat caatcctgta aggattaaat gcttcacttg
    15421 tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac aagccatctt gcgtaggcca
    15481 agggccaaac tcagcagatt gcgaattgac gaatgccatc gtattcaaat tatattttct
    15541 gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa ccagagtatc gatgaggata
    15601 aaaattatga gaacctaata aattatccgt gtttggcgaa taactcatga tgttgtcaac
    15661 gtaattcgtg cccgaactca tattgatctc gattttgata ccaaaaggat tgcgcgtcat
    15721 ttttgcttct tctgctcctg tgaatccgat atctaagcgg accccgtagg cacccatctt
    15781 atggaaaaaa gataagtcat cgtaagaaat ctccaactgt tcaaataacg ctggattgat
    15841 atcaaccatt acttccatgc ctaggctatt cgcgtaatca actacttctt taaattctga
    15901 aagaactttt tctttatcat cattgatctg cagcaaactg gtaaaaaccc gtttataccc
    15961 atacttatga gctaaatcca agtacgcttt gtccttttca aatgttgacc gttctggata
    16021 aatcgagatt cctaattttc ccatattacc atttcctcct gcatcttttc gtttttgtat
    16081 ccacttacat tatagcaaac acaaagtaaa tggtctacac cttttctttt tcttttaaaa
    16141 taaatgataa attccaataa tttaagtagg tattttccct ctattttgtt atcattaaag
    16201 ataacaaaat agtaaattgg aaaggaacag ttatgaagaa aaataaagtt attttaagta
    16261 ttagtttagc agcagcattg ctgtttgctg ctggttgttc tgcaaacaac acgacttctt
    16321 ctagttcgtc tacagaaagt tcaaaaagta catctactgc ggaaagcacg actgccagta
    16381 gcgtcgattt gaacgcattg gaattaccac aattgacgaa agatgtcgcc gaaaatgaag
    16441 atctggtcga aatggtgacg aataaaggca caatcaaaat caaattattt cccgaacacg
    16501 caccaaaagc ggttgaaaac tttatgacac atgcaaaaga cggttactac aacggactga
    16561 ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg cgatcctaaa ggcgatggca
    16621 ctggagggga aagcatttgg ggagaaggat ttgaaacaga gatcaacaac cacctttata
    16681 atataagagg tgcgttatct atggctcgct cacaagatcc taacagtaac ggcagccagt
    16741 tctttatcgt tcaaaataca gatgatatgc atgatggtct tttaaaagac gactatcctc
    16801 aagcaatcat cgacgcttat aaaaacggcg gttatccttc actagatggc cattatactg
    16861 tcttcggcca agtgattgaa ggaatggatg tcgtggatgc tattgcaaat atcgaaacag
    16921 atagttcaga caaaccaaaa gaagaagtaa aaatcgataa aatcaatata ctgcaagaag
    16981 caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag atgacaacgt atgctaggat
    17041 tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc ttttgttttg ctatatcagg
    17101 ttttatagtc aagcattttg gttgacgatg aacgtgaact cgcactcaca gactttttta
    17161 tcatctacat atgctgcaga taacgctgta ccgactgatc ccttcatttt ggtgatttca
    17221 aaatgacatt tcagcacatc tcctggaaca actttttgac ggaatttggc cttattgatc
    17281 ccaccgatat aagctctttt tcctagaaac tcttctgatt ttaggataag tatcgaacct
    17341 gcttgtgcca gtgtttctag aatcaacgca cccggcatca ctggattgtc aggaaagtga
    17401 ccttggaaaa aatcttcatt gattgttaca ttttttgtcg caacgatctt ttttcctggt
    17461 tctaaagtat ctacataatc aatataacaa ataggataac gattaggaat caattccatt
    17521 acttcagtag cagataatac gtaagacata attaaactcc ttctttcagg caattgccat
    17581 tttcattact ttgactatca aagtatcata ttcctaaata tttagcaatc tttttttaat
    17641 ttaagaaaaa atagtttgac aatcaaaaca ttttgactaa taattaagaa atcaagaagt
    17701 tattttagct attattctct atttttttac gactatcaaa tttttatcag aaacaaagga
    17761 gagtcattat gtcatttcta gaaggaaaaa aaatcgtcat tatgggcgtc gctaacaaaa
    17821 aaagcattgc ttggggttgt gctgaagcaa tgttgaaaca aggggcagaa attatctata
    17881 cttaccaaaa tgaacgaatg aaaaaatcat tagtcaaatt ggtcggcgaa gaagcattca
    17941 ctgtagaatg tgatgtttct agcgacgaaa gcatcgaaaa agcaatgaat gccatcggtg
    18001 aatatgctgg ggagatccac ggccttgtcc atgcagttgc ttacgccaac aaagaagaat
    18061 tgtccggaaa tgtgtctgat atttccagag acggctatca attagctcaa gatatcagca
    18121 gctactcttt aattgctgta tcaaaatacg cacaaccgtt tcttgcacca aacagcggga
    18181 tcgttagcat gacttatcta ggttcagaac gcgcaatccc gaattataac atgatgggta
    18241 ttgcgaaagc ttctcttgaa gctgctatcc gttatttggc agctgagttc tcgcctaaag
    18301 gaattcgggt caatggaatc tcagctgggg caatcaaaac attagctgtc acaggtgtta
    18361 aagactatca aaaactgatc cagttatctg aatctcgtac gcctgatcag caaggtgtga
    18421 cgatcgaaga agtcggtaat gcttgtgctt tcttaatcag cccactttcc agcggaatca
    18481 tcggtgatat tatctacgtt gacaaaggtg tccatctttc ataaactttt gttgcattct
    18541 ttaaagtaaa acatataaaa atcaggaaaa aactttttaa agtctcttcc tgattttttc
    18601 tttttacgtt tttttcaact cattactttg aaattattcc acgacttcaa tccagccttc
    18661 tggtgcttct atgtctccaa attgaatacc tgtcaattca tcatacaacc gttttgttac
    18721 tggtcctact tccgtttcac tataaaatac atgaagattg ttattgtgat aaatcccacc
    18781 gattggagaa ataacagccg ctgtaccaca tgcaccagct tctgcaaatt gatctagatc
    18841 atcaatgaaa atatctcctt caattgcttg cagtcctagt cgttcttcag ctaactgcaa
    18901 caaggaatat ttcgtgatac ttggtaagat agagggcgat tgcggagtga tgaattttcc
    18961 gtcttttgta attccaaaga aatttgctgc acctacttct tcgattttcg tatgtgtggc
    19021 tggatcaaga taaacacaat ctgagtaatc cagttctttt gcttctgttc ctggaagtaa
    19081 actcgctgcg taatttccgc cgacttttgc tgcaccagtc cccttaggag cagcacgatc
    19141 gtaatcagat acaataaaat tcgttggttt tagtccacct ttgaaatacg ctcctactgg
    19201 cgtacagaag acgacaaata aatattctgg agctggatgg acaccaatgt tttcaccgac
    19261 cccaatcagt aaagggcgca agtataatgt agcgcctgtt ccatagggcg gcacaaaatc
    19321 ttcatttgct tttaccactt gcttgacagc atcaacgaac atcttttcag ggaccattgg
    19381 catcagcaat cgtctagcac tttgattcat ccgtcgacta ttttgttcag gtcgaaagag
    19441 attgaccttg ccgtcttttc gacgataagc ttttagacct tcaaaacatt gctgaccata
    19501 atgaagcgct ggagagcctt catgaatcgt caggtaatta tcttctgtca attctccttc
    19561 ttcccattgt ccgtctttcc atttggcaat aaaacgccaa ggagttttga tataagaaaa
    19621 acctagattg ttccaatcga tttgcatata cttaccacct tcgttatttt ctccacattt
    19681 tatcattatc catattattt gtcattttat tttcagcttt tttccttcat tatagttatt
    19741 tttgctattt ccttttttga aaacttcttg gtacaataca gaaagaatca aattttagaa
    19801 aagaggattt tatgttttca ttattagcaa ctgccggtac gacagactct tctggcgaga
    19861 cattgacaga tcaagctgtc agcaaagtaa atgcgtttca gcggttttgg gacgggattg
    19921 attgggacgc cattttggct gcctttatcc aaaaaagttt atatctgatt tttttgatcg
    19981 tccttttttg gattcttcac cgtgtgggaa aatatctgat tgataagtct tttaatcagt
    20041 attctaaaaa agccattcta aacgagagcc gcttaaagac tttacatagt cttttaaata
    20101 ctatttttca ttataccctt ggtttctttt ttatctatgc aattttatct gctatcggtg
    20161 tgccgatcgg atcacttttg gctggtgccg gaattgcagg tgtggcaatc ggtcttggtg
    20221 cccaagggtt catgagtgat gttattaccg gattttttat tatcatggaa caacagatgg
    20281 atgttgggga ctacatcaag ctagcaaact taacgattga aggaaccgtc gtatctgttg
    20341 gtatccgtac ccttcagctg aaagcaacgg atggtactgt ccatttcatc ccgaatcgaa
    20401 acatcaccac gatcagcaat ctctctcgtg caaatatgca agtactttta gatattcgga
    20461 ttgttcctga agaaggatat gacaaaatct atgagatcat tgatcgagta aatcgtacat
    20521 tagctgaaaa ataccaagaa gagctccaaa ctgaaccttc tatctttgga ttagtggatc
    20581 ttggaaacag caattttgcg attcgtacgg tctgctacgc attgaacgga aaacaatatg
    20641 ccttaaaaga agagttcttg agctcctatg tcaaagaatt gacagcttct ggtttcacga
    20701 ttccgaacag tccaattgct gtcggaaaat aatttggaca aacgatgcaa cgaatctcac
    20761 taaaaagagc gtgggacaaa agtaaagaac acttttgtc
//
LOCUS       DACEOE010000033        20193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEOE010000033 DACEOE010000000
VERSION     DACEOE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.035"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(130..561)
                     /gene="ruvX"
                     /locus_tag="IW774_001033"
     CDS             complement(130..561)
                     /gene="ruvX"
                     /locus_tag="IW774_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1153393.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(558..824)
                     /locus_tag="IW774_001034"
     CDS             complement(558..824)
                     /locus_tag="IW774_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1153394.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1079..1804)
                     /locus_tag="IW774_001035"
     CDS             complement(1079..1804)
                     /locus_tag="IW774_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1153395.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2165..2235)
                     /locus_tag="IW774_001036"
     tRNA            complement(2165..2235)
                     /locus_tag="IW774_001036"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Gly,seq:ccc)
     gene            2352..3104
                     /locus_tag="IW774_001037"
     CDS             2352..3104
                     /locus_tag="IW774_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1153396.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3120..3599
                     /locus_tag="IW774_001038"
     CDS             3120..3599
                     /locus_tag="IW774_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1153397.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3850..5430)
                     /locus_tag="IW774_001039"
     CDS             complement(3850..5430)
                     /locus_tag="IW774_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1153398.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5423..6166)
                     /locus_tag="IW774_001040"
     CDS             complement(5423..6166)
                     /locus_tag="IW774_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153399.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6342..7118)
                     /locus_tag="IW774_001041"
     CDS             complement(6342..7118)
                     /locus_tag="IW774_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1153400.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7366..8202)
                     /locus_tag="IW774_001042"
     CDS             complement(7366..8202)
                     /locus_tag="IW774_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1153401.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8213..9142)
                     /locus_tag="IW774_001043"
     CDS             complement(8213..9142)
                     /locus_tag="IW774_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1153402.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9334..9687
                     /locus_tag="IW774_001044"
     CDS             9334..9687
                     /locus_tag="IW774_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1153403.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(9761..10417)
                     /locus_tag="IW774_001045"
     CDS             complement(9761..10417)
                     /locus_tag="IW774_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1153404.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10643..12577
                     /locus_tag="IW774_001046"
     CDS             10643..12577
                     /locus_tag="IW774_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1153405.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12663..13886)
                     /locus_tag="IW774_001047"
     CDS             complement(12663..13886)
                     /locus_tag="IW774_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1153406.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(13880..15778)
                     /locus_tag="IW774_001048"
     CDS             complement(13880..15778)
                     /locus_tag="IW774_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1153407.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(15772..16890)
                     /locus_tag="IW774_001049"
     CDS             complement(15772..16890)
                     /locus_tag="IW774_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1153408.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(16973..17806)
                     /locus_tag="IW774_001050"
     CDS             complement(16973..17806)
                     /locus_tag="IW774_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1153409.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(17799..18716)
                     /locus_tag="IW774_001051"
     CDS             complement(17799..18716)
                     /locus_tag="IW774_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1153410.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(18739..18918)
                     /locus_tag="IW774_001052"
     CDS             complement(18739..18918)
                     /locus_tag="IW774_001052"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153411.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(18943..20067)
                     /locus_tag="IW774_001053"
     CDS             complement(18943..20067)
                     /locus_tag="IW774_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1153412.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         6248 a         4002 c         3367 g         6576 t
ORIGIN      
        1 gtcaactgtt agtaggattt catataatgt ttcatttcct tggtcatcca ccaatgtgat
       61 gtgttcgtgt ccttcatggt cgtgggaatg gttatgttct gtcataagat tacctctttt
      121 cttttattat taaaataatt gcttacctga gccgtctaaa taattttgta agatcatcac
      181 agctgctaat ttatcgatga ctttttttcg tttggctctt gaagtatttg cctgctctac
      241 tagcatacgt tctgcctgaa cagtggttaa tcgttcatct tgataatcaa ctggcaagga
      301 gaatagttct ttgatctttt ctccgtatga catggaagct tctgcccttg gtccgatgga
      361 attgttcata tttttaggta atccaactac aaaccggtct acttcatatt gtgctactaa
      421 ttccttcaac cgatcaaagc caaactcttc ttctgcttca ttgatccgaa tgatctctac
      481 tccttgagct gtccagccaa aggggtcact gatagcaaca cctactgtct tagaaccgac
      541 atccaagccc attactctca taaattcact ccatgatttg agaggtagtc cttgacaatt
      601 acttccatga tctcatcgcg ttcgtgacgg cgaatcaaat ttcgagcatc acgataacga
      661 ggaatgtagg ccgggtctcc agaaagcaag tagccgacaa tctgattgat tggattgtag
      721 cctttttctt ccaaagcccg ataaacaatc gctaatgttt cgctcacttc ttttttgcga
      781 ctatcgtcaa aaccaaaacg tacggtttca tctgtaaaac ccatgattct acacctcttt
      841 cccgcaaggt aattccagct taccttctac aaccttattt tactcgaaga aagggaaaac
      901 tacaaacaaa tattccaaac ttccgaataa attaagaaac actatttgta tgtttttttc
      961 cttttttctg tagctttatc aaaagtactc ttttcataaa gtatacgcta tatttctttg
     1021 aatgtatgtt tttacacaat tttttcataa tattcataaa aaattcgaat agctgctact
     1081 aattttttgt ttttgttgct tgtcctgtct cataattgat cgtgtagcca tcttgaacat
     1141 tgtagataaa gacattgaat tcaatttttt tgtcttcaat gctttctgct tccaattgca
     1201 ctcccctggc taccagttca ttcccctcaa aatgaggagt gacacggtag cgtacatgat
     1261 gatttgtttc tttgatatat gccgcaatat cattttcgtg agcaagcatg tatggagcat
     1321 tcaacgaacg tgtccctgtc atcaagtttt taatgttgtt attttgacca gtcagctgga
     1381 aaccaattaa atggctgcga ttatataacc attgcccgtc acttaatttc ttttgtttcc
     1441 aacctgtcgg gtcaatatac aaaggctctc tcttttcagt agggaacaaa tcttttccaa
     1501 gcatagcatt ggcttgtcct actctgttca atttatcaat atttgaatag ctttcccatg
     1561 ttcctttgga aaggctaaga tcctcttttg taaattccgg ctgattccca ttgatttcta
     1621 tttcttgatg ttttccatca tattctggca catccgctac ttgagaagct tgtggtgtgg
     1681 aatcaaaaat agttgtaatc tgctgaaaga tatctgaatc tttttgtgtg gtggaaacac
     1741 cgaatgcccc tgcaatcaca gcagcgacga tccccacgat cgccttggtt gtttttttgc
     1801 tcatttcttt actcctttac taacagctcc ctaaatctta acatatgttc ccctataaat
     1861 aaatactaga aagaaataga aacagcaaaa aataaaacct gttttaagat ggcttaaaaa
     1921 tacaaaaaag agacggattc ctcgtctcta atcagtcacc actctatctg taaggtattt
     1981 attattattc aaaaattttc ttcatactca agacaataca ctgatttcac aaccgcttgg
     2041 taaacggtgc gcaaaagctt gcatctgcgg taataagtcc atccaagtta aaaacataag
     2101 aagttgtttt tgcttggctg ttcttattac ttgttgcaga gcagcttttt cacaacctat
     2161 tttaagcggg tgacgggaat cgaacccgcg acatcagctt gggaagctgg gattttacca
     2221 ctaaactaca cccgcaatac aaaaacagta tacgcctctt ttattccttc ctcaagtttt
     2281 tttattcttt acgcttctgg tatgttttgt agtaaaataa aactaatcaa tcaattattg
     2341 gagaagatgc tatgacggga aaagaattag atctgttgct ggacacttgt ttactcgctg
     2401 ggaaaatcat gatggaaagc aatgcggaga tgtatcgggt agaagatacg atgagtcgaa
     2461 ttgctttagc ttctggaaat ttccgtttgg tcagctatgt cactcaaacc ggtctcttta
     2521 ttggtcttga tcgtacctcg accatccgaa tggaacaaat taccaaccgt tcgattaatt
     2581 tagaaaaagt agtaaaagta aatgatcttt cacgtaaata tgttgcttca gagctaacat
     2641 tagaagaact ttactgcgaa ctgcaagcaa tcgaccgaga ccgttctttc tttcctgtct
     2701 ggttgcaaat cgttagcgcg gcagtgatca gtggaacaat tatggtgtta tttggcggaa
     2761 agatgaccga tttacctgtg actttagtca ttggaggatt gggatacgca gcttatctat
     2821 atagtttgaa attttttaga atcaaatttt tatcggaatt tttatcttct ttattgattg
     2881 gcatagccgc tatctacagc gtacgttttg gcttaggaag caatcaggat ctaatcatta
     2941 tcggatgtgt gatgccttta gttcctggag ttcaaattac gaacgctatc cgtgatttgc
     3001 tagctggaca ttatgtctca ggagtttctc gcggaacaga agcgatgatg actgctacga
     3061 tgatcggttt tgcgattgct tttattttcc agctttttta ttaggaaagg aacacaagta
     3121 tggggacgat atttattcag ttttctttta gttttttagc gactgcggca tttgccgtta
     3181 ttacaaatgt acctaggcgt tcattgattt gttgtggtct ttctggtgct tttggctgga
     3241 tgatctactg gatctgtgtt caaataggcg gatctgctgc ttttggttca ttgcttggtt
     3301 ctttaggagt agcattcatc agcgatcttt tctctaaacg tttgaaaatg cccgtaacga
     3361 ttttcaatat tcccgggatg gttcctctag tccctggtgg tttagcttat caagcggttc
     3421 gaaatctcgt tactgaatcc tatcgagaag cggctcatta tgcggtccaa gcaattatga
     3481 ttgctggagc aatcgcttta ggtcttgtat tatcagaagt ctttaaccat aacatccgta
     3541 atttcaaagt aaagcgtgac gcaatcatga tgaaacgaaa aaaacggctt tttaaataaa
     3601 cgaagtgtaa caaagaaaag gttgtgaaag agacgttcag ccttgagcga tgacaaaata
     3661 tctcacaaac attgcgacaa gtaaccgcag gagcaataag aagtaattat gagtattaaa
     3721 aaaagttttg taaaaatagc ttctcacatt tttacataaa attgacttaa tactgagaaa
     3781 ctgacttttg aacaccctct atctgtaaaa aaggggctgc gacatgattt ttgtcacggc
     3841 ccctcttctt tattcaaatt ttttccaaaa cgttcgttga taataaacga tccatagagc
     3901 ggaaagtatt agaactaaac cgatcacagg aatattcact aaccaaaatg gataaaacaa
     3961 agccaacatt ccgtataaga cgataagaat aatactgatg atcaatgaac ccatcatagt
     4021 tgcgactaaa cccaaacttc ctgaacctct gttaaataat tggttaatat tggtccagtt
     4081 cagcagcagc aaccgataat ctctagcgaa ataacgtagt gacaataagt agcagccgaa
     4141 taaagaacca aatagcaagc tagggataaa aaacaaaggc agctgaaaag acagtccgct
     4201 taataatgcg atcccaccag ttaaaaaact ctgcaatatc catccgaatc tgaacttttc
     4261 ttttaaatat tggttcatag agattggcaa agaacggata aacagaaaat tttcttgatc
     4321 caaagaaata agattgctga taaaagaagt ctgattaact gttaagaaag ccaatgcgat
     4381 ccctaccagg aaaacaacac cgattaactt attatctaaa tcagttaatt ttatttcgcc
     4441 tcctagggca aaagcaagaa taaaaatgat tggagttaac aaggaagaag aaagaacttg
     4501 cataatcaaa ttaggttctt ttactaattg agcattgtaa ctaaataaaa gctgatgcac
     4561 attttggttc tttttatggg ttctttttat tttgaactca ctgtttgaat cagtcagttg
     4621 ctctgtaagc tttggtacta tcagctgttt tacagcaagg aaaaatccca gagccgtgat
     4681 agctagtatt ccccaaccca ataaaccttg ttgagaaagt ggctgactgg cagcatcata
     4741 gaatggcaat aatatcgaaa tcggatgccg atctgttaac tcattttcaa tagtccccgt
     4801 ttgatgattc atccacatga ttccagcaaa tgcgatcagc atcgagctgc caagcaagat
     4861 actagaaact gtttttctat gttttttaaa cactgctgtt cgagccaaac caaaaacaat
     4921 caaactagcc agacaaaaaa gaataatcaa aaatagagaa aacaaaagga ttgagataat
     4981 gatagtaagc aaaatcccta gatcagctcg ccatccggtt aaaataaaca tgactagaag
     5041 cggaaaaaca aaaggaatga tggtcaaagc aactaccaaa atctttgctg aaaaaacctg
     5101 ttcttgtcca aaaggcagag gcaaaaatac ttgcaggtca tttccttcaa aaaagatatt
     5161 ataaatgact gatatacttt gagttaaacc taacatacca aaaagtgcca cgtaataagt
     5221 aaagaaacct ggcaatttaa caaagttcat agcaaacata gcaaatccgt aaatcagtaa
     5281 aaaaatcagc cctgaaaaaa gattttgaga caaaagatag atagttaatc gctttccctt
     5341 ttttcctttc tctcttgctt tattcgtgag ctgtggatta acatataaaa gattaactcg
     5401 aattaattct aataattgtc gcttatacat acggtccatc tcctgtcgct tgccttccag
     5461 ccattttcaa ataaatcttt tctagcgttt catcaggtga gccattcaat aattcactaa
     5521 ctgccccctg ataaatcaat tcacctttct tcaaaatagc cagtttatcg cataactgtt
     5581 gtgcggtatc caatacatgt gtagaaaaaa tgactgtttt tccttttgcg gcatgagcct
     5641 tcatcatttc tttcaaatca aatgctgctt gaggatcaag tccttgcaaa ggttcatcca
     5701 atacccaaat atctggatct ggcagcaacg tgccgatcaa aatcgttttt tgacgcattc
     5761 catgtgaaaa acttgcgatt gtttcatttt gatgactata catatcaaaa agcgctgtca
     5821 attctgctaa acgcttttct tttttttgtg tgtctaattc ataggcagct gaaatcaagt
     5881 cccaatattc tccagccgtc aattgtaaaa aaatatctgg ggaatcaggc acataaccga
     5941 ttttttgttt gatactcaat cgattttccg tcaatttcat tccatctact aaaatcgttc
     6001 ccgatgatgg ttcaataata ctaactaaac ttttaatcgt agttgatttt ccggcaccat
     6061 tatgtcctaa aaatccaaaa atttcccctt gtcttatcga gagagacaag tcttttaatg
     6121 cttgtttttt cccgtaactt ttacttacat tgatgaattc aatcatttct gtactccttt
     6181 tgtttgttat tactttttat tgtacccttc gttattggtg gagtaaatgt caaaaagagt
     6241 gcataacaaa aaaggataga aacgactagg ctgctacttg ggttgttctc ttctacagga
     6301 aaacactctt ctaacaggta agatatgagt ctttgttttc tttaaaatga ttcattttct
     6361 atcttttctt gcataaaatt ctttagttta tcaatacttc tctttcccat ctctagttgt
     6421 tctccatttg caaatgttac tatttcgttt actctgctaa tagatgtaat gttttcagta
     6481 ttaatgatat aagcttttaa atctttacat aaatactcat attcgagtga ttttttatat
     6541 tttttaattg tgccgtttac gagaatctct tcatctttca atactaggcc tattttattt
     6601 ttataacctg gcatgctcat tagataaagg atttcatcgt aatggatata gtgatcgcta
     6661 ttaccataac ggacagttgt ccaccttttt ttcgtagcat gttgaacagg atagttttct
     6721 ttttctttca ataagtaact ttggatgctt tttttatttt tttcataagt gaattctttt
     6781 atcaaataac cactaggcgt aatctttcga ttaataacag ttattgcttt attcggatga
     6841 gacgttagat aaacaatttt acacttttca ttcttttctc taagcatttc gcctagttcg
     6901 ataccgctat aataggcgtt caaatcgatg tctaggaaaa aaatatctga ggaatagatt
     6961 tcattatttt taatgaattc aacaaattgt ataacgttca atccctgaac gatctcaaat
     7021 ttccaaggac ttaataaaat tctggattct tccaaaattt ctactagcat tttttcaaaa
     7081 accaaactat catccaaaat aaatatacgc gttaccataa aaagtacctc cactcgatat
     7141 tttactatca aaatcatttc atacaacttt tcaacataca tgttaggaat aaattgtaat
     7201 tctcttcaca atcttttcca acacctttga aaccgatatc aaatactttc aggatgctgc
     7261 ttctaaaata aatatcaaac aaatgaatac atcatgcaaa aaaagccaga caaccgacgt
     7321 ccttgcgtgt cagctgtctg agctatgttt atttattttg ttcttttaaa aagaacgata
     7381 cacatactga tttttcggag cagagacttt ctcagcgttt gtaacaacta cagatgggat
     7441 ttcacgcttg aatggttgat aatagtctga ttggatggtt ccttctattt tataccattc
     7501 atcattttta accgttactc cttccggcat atgcgtcaac aaaccaaaga ctcctgaatc
     7561 tgccacacaa tggataatgc cgaatcggaa caagaagata tctgtattgc tatcgttcgg
     7621 tgtttggtaa acaaatcctt catagctgat ttttttgcca atgaattcgc tagggtaatt
     7681 ataaattagc tccataactt ctaaatagtt ttcatctgtg atcttgatag tttgcttatc
     7741 cccatacttt tctttcaact cattcatttg gtctaagtag tctgatttgt tgaaataaat
     7801 actggtatcc ggctttaagt attgtgtctg catctccgga tcccctactg attctttact
     7861 tactgggaaa ttaaatcctt tggcttctac gatcgtagtg tcgagactaa ctgttggaaa
     7921 caacgtcccc acgattactg gcaaagctag cagtacataa gcgattccac gttgataagg
     7981 ttttgacaac ccatggtcat gatcgtgctc gtgtgccttt tcttctgctt ttccgttttt
     8041 gacccacaaa tacagctgaa ctaaagccaa aagagcagat aaaatcattg aaagaatagc
     8101 taagtagcga taatggacat tgatatattg atcgagtttg ccagaaactt gcaagtacat
     8161 catcaattcg aaatacccag ataaaatcaa aaaccgcagc atattttccc tcctaaataa
     8221 ataatgtgaa tccaataacg ctaagggcaa tcagtccgat cagcgataaa ataaagcgtc
     8281 ctgtaaaata tcgtttcatc atcagtagat ttttgatatc gaccattggg ccaaagacca
     8341 aaaaggcagc tacaggtgct gtgccgaaca gacttaacaa agaagatcct ataaacgcat
     8401 ctgcttctga gcaaagagac atcgtaaagg ccaataacag catgatgata atggctaaaa
     8461 tctttgttga gccgagcgtt agcataattc tagttggtaa ataagtctgc atcgcagctg
     8521 cgattaatcc gccaatgatc aaataacgac ctgtatcaaa aaattcgtcg attgaatgtg
     8581 tcaacacatg cccagtttga tggatcagct gattttttgg ttcattcttc tcactttgtt
     8641 cttcttcatg gacatgatga tggtgatttt ctgctacttt agacgtgatt ttttctttca
     8701 atacagcgtc tttttgaaaa taggcaagcc agacaccaat cacaagggca acaaaaaagc
     8761 tgcctatcac ccgccagctt gccatctgcc aagaattgcc aaatgcgata aaagtggaaa
     8821 aaatcacgat tggattgatg atcggagcag tcagcataaa agcaaacgct gtgtgaactg
     8881 gtacgccttt tttaacaaat tgatgaacga tcggtacgat gccgcattca catgaaggaa
     8941 agaaaaatcc taagatcgaa ccagtaaaaa tagagaagat acgattttca ggaagccatc
     9001 tcttcacacg ttctggggtc aaaaatactt gtaaggcgcc tgagataaga cagcctaata
     9061 atacgaaagg taatgcttca ataacgatcg ataagaaaat cgtactcatc tgcaaaaccg
     9121 aatttggaat aaaagaaaac atgagtttcc tcctttttat ttctagttca ttcgctttgc
     9181 aaatatacgc ttttattcaa aaaaaacaag ttgctctgta aatttttaca caactcttat
     9241 tttttaccaa agcacttcaa ttgacttttt ctgttattcc cattaaatta aggtctcata
     9301 catatcactt ctatcataaa agggagaata aaaatgaaac aaaccactaa cttgactgaa
     9361 gttcaagaca tactgcaaca gtcatccgtt gccgtcattt atctttcgat gcctaattgc
     9421 agtgtttgtc acgctgttcg tccacgacta gaggaacttc tcacacatta tgatattccg
     9481 gcacttcatt tggatgcatt tgaaacacca gaagtagcta gtaaatttga agtgctgact
     9541 gcgccagttg tcctgatttt tcatcaagga aaagaagtct ttcggcaagc acggttcatc
     9601 gactttaaaa aaatccgtta tctactagat gaactgacag cagaagatga ttccctttct
     9661 tatgaagaaa tcttccgaac aaaataatcg ttgtccaaac agaaaaaagc gattgaacaa
     9721 gtgaaagttt tgccttcatt tgttcaatcg ctttttattt ttattctgct gtattttcgg
     9781 aatgcgcatt tgcgtcagaa tgcaagaaat agatcaaggt ttgcaattca ttcgtcaaat
     9841 caacattttg gatcaataca tctgacggag cattcaatcg agcagcggta aagttcaaga
     9901 ttcctttgat tcctgcttcc gttaactgat tgactacttc ttgtgctctt tcagcgggta
     9961 tcgttaaaat tgctacttcg atttgttgga tcttgatttg ttctgacata tcttccattg
    10021 gataaacggg gataccgtct acgatgcgcc caacgatttc ctcattcaca tcaaaagcac
    10081 aactgatacg tatgctatta ctttgatgga atttgtattt tagtaaagca cttcccaagt
    10141 tccctacacc gatcagcgca acatttgtca attgatcgtc gtttaatgtc tttgcaaaaa
    10201 agtgcataag atcttctaca tcataaccat acccacgttt tcctaattcg ccaaaataag
    10261 aaaaatctcg acggatcgtt gcactatcta cttgaacggc ttcacttaat tcagttgaag
    10321 acactttttt ctttccagca ttatttaaaa tccttaaata gcgataatac aaaggcagtc
    10381 ttttagctgt cgcctttgga ataatatgat ctttcacgaa atgacctcca cgtattgtct
    10441 aattacatct ttgatttctc catcatagca gtatttatag gaaaaaatca attccattgc
    10501 tcagatattg tgaaatttta ttctttattc atttatttat catttctgca aatctcgcag
    10561 tataatggaa tttatggtag actactaatt gaaaaatagt tttttatttg aggagaattc
    10621 acaaagtaga aaagaggatt ccatgatatt attacaagcc aatcagattg ctcgccattt
    10681 tggtgcagaa gtcttatttg aaaacataca tttagaaatc agtacaggcg ctcgtgtcgc
    10741 tttagttggc agaaatggcg ctggaaaatc gactttatta aagatcattg ctggaattga
    10801 agcacctgat gaaggaaaga tcgctaaaaa taaaacagcg actctcggat acctcgctca
    10861 ggacacagga cttgattcca atgaaacagt ttggaacgag atgctgaaag cttttgaaca
    10921 agttagacaa atggaagagc ggatgcgcga gctagagatt ttcattagcg aaggtacgcc
    10981 agacacacct gcttatagtt cttatttaaa agaatacgac cggctgcaac acgatttttc
    11041 agatcgaaac ggctatggtt atgaaaatga aattcgttcc gtattgcatg ggtttaaatt
    11101 cgattcttca ttttatgatc agaaaatcga tactttatca ggcggacagc gcacacgttt
    11161 agctttagct cgcatgcttt tgcaacaccc tgacattttg attctagatg aaccaacgaa
    11221 ccatttagat atcgagacac tttcctggct tgagaattat ctgcaaagct atactggtgc
    11281 attattgatt gtttcccatg accgttattt tttagataaa gtagtcaatg aagtctatga
    11341 attaagcaga agaaaaatgc gccattacaa aggtaactat tcgaaatatc tagatttgaa
    11401 agcagaacag ttagcaagtg aatggaaggc ttatgaaaag cagcaaacag aaatccataa
    11461 attagaagac ttcgttgctc ggaaccttgt tcgcgcctct accacaaaac gagcgcaaag
    11521 ccgcagaaag acattagaaa aaatggaccg gctggatcgt cctcaagggg atgaaaaaca
    11581 agcaaacttt ctattcagta tagaaaagcc ttctggaaat gtagtcttgc aaatcgaaga
    11641 tgccgctatc ggatatgata atgtcacctt atctgaacca atcaatctag atatccgtcg
    11701 tcaagaagcg attgctctag taggtccgaa tggtatcggg aaatcgacgt tgctgaaatc
    11761 aatcattcac cagatccctt ttatccgtgg aaaagaaacg ctagggacaa atgtcaccat
    11821 cggctattac gatcaaggtc aagctgatct gcatacaaac aagacgatcc tagcagaatt
    11881 gtgggatgag catcctacca ctccagaaaa agagatccga agtgctttag gtgctttctt
    11941 attttctggc gaagatgtag aaaaaccaat ccctctgcta agtggcggtg aaaaagcacg
    12001 ggttgcttta gccaagttag cgatgaacaa agaaaatttt ttgattttgg atgaaccaac
    12061 aaaccattta gatattgaca acaaagaagt attagaaaat gcattgatcg attatgacgg
    12121 cacactgctt tttgtttccc atgaccgata tttcatcaat cggatcgcga caaaagtcgt
    12181 cgaactctct gaaaatggca gcaaagtcta tctaggagac tatgattatt atttagagaa
    12241 aaagcaggaa gaagaagaaa tcgccgaatt gttggcacaa gatgagccag aatctgctcc
    12301 tgctcccacg aacaaatctg attattacca aagcaaagaa cagcaaaagc ttatccgtag
    12361 ccttcaacga aaaattactc aggttgaaga agaaatggcg cggatcgatg aatggatcga
    12421 tcagttggaa caagaaatgg tcgcgccaga aaacctggaa gatcacgtaa aactcaatga
    12481 gctgaaccaa gatttagaag ctgctagaca agatcaagaa acaaaattga cggaatggga
    12541 agaactaagc ttggaattag aagaatatga aaattaacga aaaaaccaaa acagaaacca
    12601 ttcatgctgt tttggttttt ctttttttat tttatcaaac aaaaattcca tatttacctt
    12661 tttcaccaat aagaagacca attactagtc attctcacta aagcttctaa ataatagtaa
    12721 tcaccccaaa tacaaggtgt atttacccca ttatttttgg gcttattata aacagactgt
    12781 gttaaaaagc catcttcttc ttccgttgta gcatatcttt ctttcaattg tcgcataatt
    12841 ttccaaacag cagactggta taagttcttt tctgttttgt ctaatagacc tgattctatt
    12901 aattctaata atccacaaac catgatcacc atactagatg tatctttttc ttctctgcta
    12961 gaatcatcaa agatcaaatc ccaataacaa atggtatctt ctggcaaccg attcataaaa
    13021 taattggcta atattttgct taaatcaaaa tatttttgtt ctttcaaata acgataactt
    13081 aatgcaaaac cgtaaatccc ccatgcttgg cctctagccc aacaggaatt atcactataa
    13141 ccttgatgcg tacttccata tcttggtttt ccatctttga catccatata aaatgtatga
    13201 taagttgaac catcttctct tactaaataa tctgctgcat tttgaatatg agcctgagca
    13261 gcttttttat atttttgatt tccagtttct tcactcgccc aatacaataa tggaagattc
    13321 atgcaacaat ccatgatcat tctaccttgt tgtgaaggat cactaggatc tccccaagct
    13381 tgtataactc ctgccttctc ccaaaaacgc tgaagtaatg tttctgcagc atcaattgaa
    13441 gcttccttca tttttcgatt tcccgttatt ttataatctg ctacggttga taaagaatac
    13501 aaaaaaccaa gatcgtgatg attagtgata atttgacagt caatcctgtg gataaaatct
    13561 atatctgctt tttcagctgt ttgttgaaat aaaggatcac ccgtcttctc aaataacaac
    13621 caattaattc ctgtccaaaa tccttctgtc caaccaatat tgtctcttaa tggataacgt
    13681 ccttttatag gacactcgct tgggaattga tactgactgt tactgatacg tttgattaac
    13741 ctctccgtgt gtctgcgaca acgctcgatc tcattattcc attctttttc cattaattct
    13801 ttataacatt catttatcca tttttctgga tgtattatct cttcaaacat cactttatca
    13861 agcatttccc caccagcctt tagcataatt ttagtctttt tttattgttt ttattttgga
    13921 tagttagccc ggttgtccca tcaaaatcaa ttttgggacg attgttccat acttctaaac
    13981 tataatcccg atcttgatga gcaaccactc ctgttccgag ccaatgggtt ccttttccaa
    14041 atgtaccctc taatccagga ataaaagtgt tttcccatgt catcaagttt gtattgggca
    14101 aacccataat agctgtggat tttcgtttaa tagtatcgtc acttaaacag ataatacctg
    14161 aaaagccagc ttcactcacc ctatagtttt ccttgtcttc ttctatgatt attccttctt
    14221 tctcaggaga acattcaata gcaaaccctg tctcatatag aacatattct gtatctaatt
    14281 ctactttgtg tacgcgaatc tgccagcctt caaaaggaat aagccaagtt ctgatttttg
    14341 cctgtaaagt aggttcccat gtagaggcaa gtacctcttt cttacataca atgtctcgta
    14401 cattatttcg aacgatgaac tgtccatagg ttttgatgct ctcattctta aaactggtcg
    14461 tgatatatgt gtccgctcgc tgaatcccta gcattgaatc cattgccatt tctgctttca
    14521 attgattcgc tcgcggtatc gaaaaaccaa attgactaga ataagcaaat tttaaatatt
    14581 tttcattggt caatgtatgg aaatttggcc ctaattgtcc cgcattcaaa aaaaatgtat
    14641 gtccattatc atgttgtatg agcaaattgg cttctgaaag aagtttagtt ccttcacgaa
    14701 taggcatagg catttcttta gccgtccaat actcatggtc ttcaggtagc gccaacaata
    14761 aaaaaatctt attcagccat aatggagata acgtactatt atagggttca gtcaatgtca
    14821 attgagggta ggtataacca atattcagga tacctttttc atcaaatatt tctttttgca
    14881 tccaccatct tatattccga ttgactaacc ccttgatttc acctaattta aaaggcaaaa
    14941 gatcggccca caacatagct gaccaaaagc ttgaaactgc aaaacgataa atcatgcttc
    15001 gaccaaaagg cacatttgca ccatcttccg caaaaaaatg aatatagtct ctagaaaaat
    15061 caatagctcg gttcttatat atctggctaa tgggcttttc tggttctagt acgctataaa
    15121 tcaaaccata ataatgaaat gcaaaaggat tataataatc ttggcgacct cttgaactgt
    15181 ctcgatacca gccattttga agataacaat tttctatatt atcaaatgct tctttcagtg
    15241 ctgattcttt aactggataa ccaaattttt ggataattgt tccgacaatt atcttaaaaa
    15301 attgccaatt accatctgca cattcatatt caaaaatttg cgagaaccaa tgaataatct
    15361 ggcttttttc tcctgtatct aattttttcc ataataattg ttcatgatga tataacgcta
    15421 ataacattgc gggcatttct accatacgct ggtctctttc atgtatttct ccccaataat
    15481 cgggatggga aggatctgtt ccagctttta ttcctgatag aattaatttc aaccatcgtt
    15541 catctaattg gtcaattgcg ggccccgctc cccataaaac ccgagagaat ccttccattc
    15601 ctgcaatttt atttccatat cctacccctt gatgtatata atcaatttgt gcgccacttg
    15661 ctcgataaaa cggctggagt ggttgattta tctttttaaa taagtcaaag taatcctgtc
    15721 tggtccttaa tagtggtact ggatgtttca ttgtattttc tcctcttttt actaccaaat
    15781 agctaattcc gactgttcta atcgaagtaa gccttcaata aaaaaataat cagaatagat
    15841 cagtggcaca ttataagtca gttctctatg gtatgcttct gctccgcctc caatgatccc
    15901 atcttcatcc atattccaat ttgcatagtg cttttctgtc ttttggagta tattgatagc
    15961 agcttcatga tacatttttg atcgtacaga ggaaacatat cctgctaatt ccagaagacc
    16021 gcatgcagcg catactccag cactggtatc aatattcgaa tcttttacag caaaatcaat
    16081 gagaggaata taaccagttt tttcgatttt ctgaataaaa tttactgcta tcttttctgc
    16141 tatagctaga tagtacggat tttttgtgta acgataactt aacgcgaatc catataaagc
    16201 ccatgcattt cctctgctcc aacaagaatt ctcattgtat ccttgcccgc caagtaaccg
    16261 aatatatttc cctgattctg catcaaattc tgcgatatgc ccggttgatc catctttcct
    16321 cactagatac ttagctgttg tatctgcatg ctgccgagca attttagaga acctcggatc
    16381 accagtttcc ctcgaagccc aatacagtaa tggtaaattc atcatactat caataatcat
    16441 ccaaccaggt ttatggtcat tccatgctac taaatagttc cctgaaatat tatagcgtcc
    16501 agcaagtacc gtagctgcat gtaaccctag ttgtcgacta tgtttgtttt gagactgttg
    16561 ataatgtgca atagccgatg gtaagaataa aaaacctaca tcatgatgaa cgtctgaaaa
    16621 atggtacagg gcagattcta ataattgttc ttgttgtatt gctgcctctt tgaacttttc
    16681 tcgttttgtc ttgtgaaaga gctgccataa aataccactc caaaaaccat tggtccacca
    16741 gtcgattcct ctgtccttta ttacgtctgt atattttccg ttgtaagaaa tatatggaat
    16801 tttattcccc attctgtcta attccgcatc tatcttttca gaagctacat tcaaaatttt
    16861 tcttgaccat tccttcgtct gttcattcat ggatatcccg ccattctttt aatttttatg
    16921 aatgtggagt tgctcctaga acgaactaga tggttaacat tcgttatttt ttttacttat
    16981 ttgcagctcc aatgtcattc tccttaaatt tagactgcgc ccacacataa atcaagacca
    17041 ttgggatcac tgaagttgct aaaattagca taattgcgcc ataattaatt ggctcacctg
    17101 tactagaaac cgtcatattt ttgataatta agggaaccgt atatttatct ttatctgaaa
    17161 gcatgactaa tggaaataga tattgattcc aacttcccat aaagataagt aaaccactag
    17221 taataatcgt aggtgataaa atcggtacag cgattttcgt aaaaatcacc cactcattgg
    17281 caccatcgat tctggcagcc tccatcagtg ctgtaggaaa ttgactgcta tattgtcgca
    17341 tcataaacac tgaagttgta ctggcaatcg aagggatgat cacacctgca taagtattcg
    17401 ataatcctaa acgactcata atgtaaaata aaggaatcaa tgtggtaaaa aaaggcacca
    17461 tcctagatag cataactgta ttgaacaaca gtttattccc cataaaacga tactttgcta
    17521 atgcataacc cgccattgta gaactggctg tagctaaaac tgtactgatt acagcaataa
    17581 caatcgaatt ccaaataatc aagggataat tatatctcgc catgatggct tgccagtttt
    17641 ttaatagcat atccccaaac ttgaaattga ttccgccact taaaatttct tgattacttt
    17701 gcgttgaagc tacaatcata tataaatatg gcatcaaaaa tattaaggca ccaaacacaa
    17761 taatgacgta cgtaaaaatt ctgctttgta tcttttgttt agtcattttt atcacctccg
    17821 aatttaaatt gaatagccga taatataaca gatacaaaca ccatcgtcca ggcaatagca
    17881 gaggcttttc caaaatcaac catttcaaaa cttgttttat ataaattgac tcctacgacc
    17941 aaagctgaat tttctggtcc attggaagtc ccaaagaata tatttgggat attgaaatta
    18001 cccaagccat tgatagtggc taaaactgtg gccattaaaa tgattggctt tagcatgggg
    18061 atcgttaata aaaagaattg ccgaaatttc gatgctccat ctatttcagc actttcgtat
    18121 atctctttag aaatcccttg aagaccagct aaaaataaaa tagtgttata tcctacacta
    18181 agccaaatca tcgtgatgat cattgatatt cgtatccaaa aaggatttgt taaccactgt
    18241 acaccgttaa ttcctagcgc attcaaaaat ccattgaata acccagtcgg attgaaaaaa
    18301 gtcatgaata cggaagctac agccaccgtt gatgtaatgg ttggaatata atagacagtt
    18361 ctaaaaaatc cttttccttt taactgtgct gaattcaata aaacagcaaa gtaaatagct
    18421 aatagcatcg ttattggaat tgatcctgcc atgataatca aaacattcgt gtaagattta
    18481 ataaacaatg gatctcggaa gatagctaga taattgtcaa atccaacaaa ttcaaatgag
    18541 tagccaattt tcataaaact aaaagcaaac gagataatga tgggaatcaa aatcgtcaca
    18601 ataaagaaaa taacaaaagg tgtgaccaat ccataagcac tttttttatg agacgtatct
    18661 gttgcttttt tcttgctgtt ctttattgtc ttgcttggta acgaagattg catcatcgtt
    18721 aagtcctcct aatatttttt aatcaccagt taattttacg ccgtacttat gagcaaaatt
    18781 ttctgcttct tttagatatg aattttcaaa atcacctgtt tgccaatagt tatatgcagc
    18841 ggtagtcaaa taagacgtag catctgaata agtggctggt aaaacagtat ttgatgtatt
    18901 ttcattgatt ttattcaata gttcatagta tttttgtcct ccttagtagt caaacttttt
    18961 ctcagctgct tctgtttgat aggccaatga gttagatgta gccacgccca gctccagtgc
    19021 gttggctaag cattcttcat ctgtcagcat aaatgtaaga aattgttgtg ccacttgtgg
    19081 atttttggca tctttaggga cataaaaact tgatcctcct gatactgcac cttttcctgg
    19141 attttctttt gaaaaaggaa tcagtggagc catgcgccat ttgtcagatg catcaggaaa
    19201 attcaaagac atattcgtag cataccagcc gccagcaaca aacattgcag attcttgaaa
    19261 tgctttctca gcatcttgtg caccgtagaa atgtacaata tctgagtcga tcatttcttt
    19321 gattaacttt gcagcatgtt tcgcttcttt agtattcaga cggatatttc cttcttcatc
    19381 aagcaaagat tgattctgct gggctagcat gttgataaac aattcttggt ccccagattc
    19441 tggcaaggca attccaaaac taccagtttt ttcttttaat gcttttgcca cctggatata
    19501 ttcttcccaa gaactgattt cttcaaaatc tacacctatt tcagcaaatt tttttggttg
    19561 ataataacaa agtgctgggg taaaatcact agcccaagga ataatcgttt cattactcgt
    19621 ttgttgacgc aaaatattca atttttgatc ataaaactga tcgccatatt tgtcataaaa
    19681 tccatattca tcagcactat aaaaagcatc ttgaaatttt tccagcacac ctgctgctga
    19741 agtgtctgcc atggagataa tatccggcat atcttcgtgt gttaccaatt ttggggtgat
    19801 catttgtact gttgctgttc ccgaagctac attcacaatt tttaagtcaa tatttgctcc
    19861 agttttgcgg ttatagattt ttgcagcatt ttcaacatag tttgtcagtt tgccgtttgc
    19921 gacatatact tctaatgtag tggctttttt tatcattgga tctgcgatgt ttgctgttac
    19981 ttcttcgtta gtttttccac atgcacttaa gctgaataat agggtaatgt ttagcaaagc
    20041 tatcaccaat ttgtatcttt ttttcatgtc tatcccttct ttcttttcaa gttcttatat
    20101 aaaacgcttt cacttatgta tattaacgga attagtaaca caaatattta tataaaaaaa
    20161 tattttactt gtcgttttgg cttttcattt ttg
//
LOCUS       DACEOE010000034        20173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEOE010000034 DACEOE010000000
VERSION     DACEOE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.036"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(13..996)
                     /locus_tag="IW774_001054"
                     /pseudo
     CDS             complement(13..996)
                     /locus_tag="IW774_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            complement(1001..1648)
                     /locus_tag="IW774_001055"
     CDS             complement(1001..1648)
                     /locus_tag="IW774_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1153413.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="IW774_001056"
     CDS             complement(1733..2365)
                     /locus_tag="IW774_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1153414.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IW774_001057"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IW774_001057"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1153415.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="IW774_001058"
     CDS             complement(2929..3666)
                     /locus_tag="IW774_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1153416.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="IW774_001059"
     CDS             complement(3663..4652)
                     /locus_tag="IW774_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1153417.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4910..5452
                     /locus_tag="IW774_001060"
     CDS             4910..5452
                     /locus_tag="IW774_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1153418.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5469..6554
                     /locus_tag="IW774_001061"
     CDS             5469..6554
                     /locus_tag="IW774_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153419.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6566..7372
                     /locus_tag="IW774_001062"
     CDS             6566..7372
                     /locus_tag="IW774_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1153420.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7373..8200
                     /locus_tag="IW774_001063"
     CDS             7373..8200
                     /locus_tag="IW774_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1153421.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8197..9270
                     /locus_tag="IW774_001064"
     CDS             8197..9270
                     /locus_tag="IW774_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1153422.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9698..12394
                     /locus_tag="IW774_001065"
     CDS             9698..12394
                     /locus_tag="IW774_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1153423.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12471..12959
                     /locus_tag="IW774_001066"
     CDS             12471..12959
                     /locus_tag="IW774_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1153424.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            12971..13465
                     /locus_tag="IW774_001067"
     CDS             12971..13465
                     /locus_tag="IW774_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1153425.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13486..14295
                     /locus_tag="IW774_001068"
     CDS             13486..14295
                     /locus_tag="IW774_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1153426.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14292..15113
                     /locus_tag="IW774_001069"
     CDS             14292..15113
                     /locus_tag="IW774_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1153427.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15164..17263
                     /locus_tag="IW774_001070"
     CDS             15164..17263
                     /locus_tag="IW774_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1153428.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17260..18378
                     /locus_tag="IW774_001071"
     CDS             17260..18378
                     /locus_tag="IW774_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1153429.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18417..19295
                     /locus_tag="IW774_001072"
     CDS             18417..19295
                     /locus_tag="IW774_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1153430.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19486..19638
                     /locus_tag="IW774_001073"
     CDS             19486..19638
                     /locus_tag="IW774_001073"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153431.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         6168 a         3476 c         4300 g         6229 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagctagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatacgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
     4681 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     4741 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
     4801 gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact aatacgagtg
     4861 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
     4921 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
     4981 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
     5041 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
     5101 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
     5161 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
     5221 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
     5281 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
     5341 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
     5401 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
     5461 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
     5521 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
     5581 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
     5641 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
     5701 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
     5761 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
     5821 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
     5881 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
     5941 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
     6001 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
     6061 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
     6121 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
     6181 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
     6241 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
     6301 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
     6361 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
     6421 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
     6481 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
     6541 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
     6601 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
     6661 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
     6721 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
     6781 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
     6841 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
     6901 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
     6961 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
     7021 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
     7081 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
     7141 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
     7201 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
     7261 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
     7321 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
     7381 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
     7441 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
     7501 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
     7561 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
     7621 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
     7681 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
     7741 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
     7801 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
     7861 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
     7921 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
     7981 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
     8041 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
     8101 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
     8161 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
     8221 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
     8281 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
     8341 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
     8401 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
     8461 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
     8521 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
     8581 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
     8641 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
     8701 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
     8761 tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa attgaatcat
     8821 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
     8881 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
     8941 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
     9001 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
     9061 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
     9121 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
     9181 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
     9241 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
     9301 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
     9361 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
     9421 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
     9481 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
     9541 tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc tatgaaaaaa
     9601 cataaaaatc atattagata aaaactaaat tggtagataa attattttgt cagatatctg
     9661 aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat atagaataga
     9721 catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg aaaaaatgaa
     9781 aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga atatgtcttc
     9841 tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg ccagccatta
     9901 tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc ctgttttgtg
     9961 gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg aagcctttca
    10021 ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta tagccgctgt
    10081 caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg ttggaaaaag
    10141 ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg aaaaagatgc
    10201 accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc tttcggcaac
    10261 actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg atggtttatt
    10321 ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat cgaaagaaaa
    10381 tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg gtgaaaatgt
    10441 aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag gtgaaaatta
    10501 tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa cttttgaaga
    10561 tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg aagcggatgt
    10621 tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat ctcatccctt
    10681 tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg atgtgtatag
    10741 tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc agttaaatat
    10801 gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga aaaatcaaaa
    10861 gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa aaattactca
    10921 gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg aagtacagca
    10981 tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat tgatgattca
    11041 gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga cgacgagtgg
    11101 acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata agacagactt
    11161 aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact tatgtaatca
    11221 gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa gactgcttat
    11281 tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc tgttgcttgc
    11341 tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat gcggcaatta
    11401 tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg tcgaatcgac
    11461 aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg tagatatggg
    11521 ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt tacttcttct
    11581 gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga tgaatgcgcc
    11641 atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc aatactttga
    11701 aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg ggatttcgga
    11761 caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag ttttcacgaa
    11821 ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt ttgacaagac
    11881 aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta atattaatgt
    11941 ttatgacatc ttagacggtg atggagcagc caatttggag catattttga gtcctgaaat
    12001 gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt cgttggaagg
    12061 aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag tggaaacagt
    12121 catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa aactaaatct
    12181 ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag aaaaatctga
    12241 agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt ctagaagtat
    12301 ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc tttcgctgct
    12361 ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg tctaatctgt
    12421 tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca atgtttttaa
    12481 aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt aaccatatga
    12541 agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc agtattcaat
    12601 tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat gagtgtgatt
    12661 ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag atttttgttt
    12721 ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt ttggagaaag
    12781 agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa atagtattgg
    12841 caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc caacaggaat
    12901 taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat tttttttagg
    12961 aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg gacaagtagc
    13021 gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg atgaggcggc
    13081 taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg tcaaagtcat
    13141 tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga aggacatgcg
    13201 cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag ctttcccaac
    13261 ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat cggaagataa
    13321 gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact taaaagaatt
    13381 agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa aacgttcagc
    13441 tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta tgatgcattt
    13501 gtagtcgctt tggccgtttt tattggagtt gctggacatg aaatgttcgg gatgtccatg
    13561 ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga tttgcagacg
    13621 gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa tatcggaatc
    13681 tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc aatcaaaatg
    13741 ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt aggattacag
    13801 ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt tttcaaattt
    13861 gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg gtttgtccac
    13921 tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga tgttgtcaaa
    13981 agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc tggaaatctg
    14041 ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa tattgctgtg
    14101 tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat tgcaattgcc
    14161 gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact aaaaaatata
    14221 tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca agaagaggag
    14281 gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa ttggttcgga
    14341 agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg atgggcggcg
    14401 gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt gaagaagatc
    14461 gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct tgcgcgtcct
    14521 ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct ggttttgacg
    14581 tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga attggcgatt
    14641 cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac ttagccaatg
    14701 ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct aattacttga
    14761 tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt gaaaagctgt
    14821 atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt ctgatcatgg
    14881 taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt tcaatgaagg
    14941 gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga attgtaccgt
    15001 tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc atcaaaatta
    15061 tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca tagtaggaag
    15121 ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta ttaatgaagt
    15181 aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa atgtgactac
    15241 ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga ccttcgatgt
    15301 aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa cgttgacctt
    15361 ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata aacagctctc
    15421 tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc atttgtcaga
    15481 atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat atattcatca
    15541 ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta ataagcgttt
    15601 aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc ttcaagtttc
    15661 agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg tgttgatttc
    15721 tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg ccattgcttt
    15781 tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata tcgaaaaatg
    15841 gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa tgccaaacga
    15901 tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt tcggtatgaa
    15961 tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta atcgaatttg
    16021 gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg agttatgtca
    16081 aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa aatttacagg
    16141 aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta gtctttattt
    16201 tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac tgaatgcttc
    16261 aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc tttttccatc
    16321 tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca atctttgtct
    16381 ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac tgagtcttag
    16441 caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac aaatgactat
    16501 cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga aaacaaccta
    16561 ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt taagtgatgt
    16621 gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag acactacgtt
    16681 gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca ccatggcctt
    16741 ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca cttggggatt
    16801 agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc tgcacaaccc
    16861 tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag acggttcgtg
    16921 gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag actttggctg
    16981 cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc aatatttcgg
    17041 attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa cgttttcttg
    17101 gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat ccgagttgag
    17161 tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa ttcttcacgt
    17221 agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact aaaaaatatt
    17281 acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac aggcgagaaa
    17341 ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg tggcgcaatt
    17401 ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg aataattcgt
    17461 catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt acgtgattta
    17521 atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc tttaggggat
    17581 ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat agtggttaat
    17641 cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc taaaattacg
    17701 atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa ttacgctttg
    17761 gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg ggaacgcatt
    17821 accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg ctattacgtg
    17881 ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg cgagtttaaa
    17941 ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc agaagattat
    18001 tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca tgcgaaaaac
    18061 tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac aagagatcgt
    18121 tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat gccacgacat
    18181 ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt atcggtcact
    18241 ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga tgttgctagt
    18301 gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt cactgatcga
    18361 gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa cctatgatga
    18421 tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt actgaggaat
    18481 taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca atgaagcagg
    18541 tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc ggctgctttg
    18601 gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca acaccaaaac
    18661 ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat attccgattg
    18721 aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt tctggcaaag
    18781 ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt gccttgttca
    18841 atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg atcaagcgac
    18901 atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta gaggggatgg
    18961 cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta ttggctgacg
    19021 attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat acaacactga
    19081 tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa cacatgaaga
    19141 aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata ccaccaattg
    19201 atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc ggattagcat
    19261 tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca ttgatactca
    19321 tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa agcgaaaatg
    19381 aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca ctgaaaggaa
    19441 atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag gatattcact
    19501 ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt aatagttata
    19561 gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct tggcttaatg
    19621 tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta attctattat
    19681 agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta tacattttaa
    19741 atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttac ctttaactct
    19801 cgcttctctt gcaaatgtag ttagcatttt aatcgataat aaactcaggt agttatatta
    19861 ccttgaaatg attgataatt ttgtcacttc tatttttttt gcaataaatt ttttatttta
    19921 gtgaaaaata agaaactatt tctttgaagg cgataagtta gaaaaatatt attgtagtct
    19981 tttctttatt aggaatcatc actgttgtat cttattacct tttctgtttt tcttattaga
    20041 gaaaaataat ttgagaggta gatagttttt attggaggaa gtaattatgt atatttttga
    20101 aacaaaaatt tttcattgca ataattgcaa caaagaaatt tcatcggggg aagaggtttg
    20161 gacaaaaatg gaa
//
LOCUS       DACEOE010000035        19993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEOE010000035 DACEOE010000000
VERSION     DACEOE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.037"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..816
                     /locus_tag="IW774_001074"
     CDS             4..816
                     /locus_tag="IW774_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1153432.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IW774_001075"
     CDS             813..1613
                     /locus_tag="IW774_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1153433.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IW774_001076"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IW774_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1153434.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IW774_001077"
     CDS             3110..4819
                     /locus_tag="IW774_001077"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1153435.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IW774_001078"
     CDS             5031..9383
                     /locus_tag="IW774_001078"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1153436.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IW774_001079"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IW774_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1153437.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IW774_001080"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IW774_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1153438.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IW774_001081"
     CDS             11221..11514
                     /locus_tag="IW774_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1153439.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IW774_001082"
     CDS             11511..11822
                     /locus_tag="IW774_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1153440.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IW774_001083"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IW774_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1153441.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IW774_001084"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IW774_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1153442.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IW774_001085"
     CDS             complement(14623..14844)
                     /locus_tag="IW774_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1153443.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="IW774_001086"
     CDS             15292..17655
                     /locus_tag="IW774_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ1153444.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="IW774_001087"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="IW774_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1153445.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="IW774_001088"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="IW774_001088"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1153446.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6862 a         3511 c         4388 g         5232 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg caactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcattaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgatc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg acagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaaa cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgcct atttgtattg gaaaagccac
    18181 aaaagttatc gagtatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaagaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aagatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtgaaac gattcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggcaaagt tgtcagtatc tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtact
    19021 gggatttttt gacggcgtgc atattggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catcgattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgaggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacagttc atttgcctaa
    19621 agaaggtgtt tatgtatcgg aaataaaaat tggggaaact tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagaat tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcagta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaaaa aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aatttcaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc aca
//
LOCUS       DACEOE010000036        19935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEOE010000036 DACEOE010000000
VERSION     DACEOE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.038"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(481..836)
                     /locus_tag="IW774_001089"
                     /pseudo
     CDS             complement(481..836)
                     /locus_tag="IW774_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1020..1427)
                     /locus_tag="IW774_001090"
     CDS             complement(1020..1427)
                     /locus_tag="IW774_001090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153447.1"
                     /translation="MSRFFKFFSLLGVMLIGLSTLVGAGTSVYAAEKVNEVPDIETQR
                     QQAVESLKFYLEEAGYVDTETHQYIVTDLEAIQKKAELDGSEGIAGKFILENFVQPTG
                     KRQKRDLQKYAACVVMNSLPFGVLFGIYLIVKI"
     gene            complement(1928..2122)
                     /locus_tag="IW774_001091"
     CDS             complement(1928..2122)
                     /locus_tag="IW774_001091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153448.1"
                     /translation="MKDQKTNNKTGERSACSWAFTGAGLLHSGIYTAAALGLSLTGVG
                     AAVIPFAVGTAYGIAGAAIC"
     gene            complement(2136..2474)
                     /locus_tag="IW774_001092"
     CDS             complement(2136..2474)
                     /locus_tag="IW774_001092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153449.1"
                     /translation="MKRIVKIFGILGVFLIGLSTFVSAGISVDASETNNFLVKQQETE
                     INIIQNNLATHKDSKGNRLVYIIDESDLKSSLDRINSEITVSEIQQYLNEFNRSIQKK
                     WPWMANRSAS"
     gene            complement(2518..2850)
                     /locus_tag="IW774_001093"
     CDS             complement(2518..2850)
                     /locus_tag="IW774_001093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153450.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRKEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLVWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(2864..3094)
                     /locus_tag="IW774_001094"
     CDS             complement(2864..3094)
                     /locus_tag="IW774_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153451.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKVYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(3316..3852)
                     /locus_tag="IW774_001095"
     CDS             complement(3316..3852)
                     /locus_tag="IW774_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153452.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLTTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDYMT
                     DRCIKDNSIDVDPHVQMKTTGKLHQVTQDIENPDQYLLFGEETTNEVTQFVLLQLKVD
                     QEAQKIDAIKIYYVRSVY"
     gene            complement(3873..4964)
                     /locus_tag="IW774_001096"
     CDS             complement(3873..4964)
                     /locus_tag="IW774_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1153453.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNWE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESTYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKDCYDYYGFLVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSDGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(4972..7182)
                     /locus_tag="IW774_001097"
     CDS             complement(4972..7182)
                     /locus_tag="IW774_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153454.1"
                     /translation="MAYIKRKEKKWCVWLFLTVGFGLFFVGGLVYAVEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPAFDYMIMNTLKSFLSLMFQKSFYSVIILVAFLIY
                     NIVDDLILMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGGQVINPFELGGRSNQDLPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIHQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQRQIQRSSESKPSISVP
                     KSLQPAFSSFNNPVEQSKKIEKKNETLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(7198..9726)
                     /locus_tag="IW774_001098"
     CDS             complement(7198..9726)
                     /locus_tag="IW774_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1153455.1"
                     /translation="MVLVKEPVKALKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFYDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRMPKEHKGLLQLEDELGTSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTTIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAIGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQIEEQDTLIMQ"
     gene            complement(9780..10190)
                     /locus_tag="IW774_001099"
     CDS             complement(9780..10190)
                     /locus_tag="IW774_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153456.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFFIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFANKYVYLED"
     gene            complement(10203..10421)
                     /locus_tag="IW774_001100"
     CDS             complement(10203..10421)
                     /locus_tag="IW774_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153457.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(10418..11410)
                     /locus_tag="IW774_001101"
     CDS             complement(10418..11410)
                     /locus_tag="IW774_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1153458.1"
                     /translation="MKKRQLRRKEKVYTEKVPNQRKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSQSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQEILKKEFYAQNFIQEPGSVSGIRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMDPENKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKTFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDRKGDERK"
     gene            complement(11489..12400)
                     /locus_tag="IW774_001102"
     CDS             complement(11489..12400)
                     /locus_tag="IW774_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1153459.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLSDVETELNGGTTEKPAEPEHLQPEKPEKEPVAPEPEIPESTLPTDPIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSETNTDSSSTEQPTMSFTSEEPKEEPKQPAEKSEAIPK
                     ENKPIVPAKEVTVSVTPNGEITTNNSQGMSVPIVTSNVEELTHIPTPTTPLKAEGGQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVLS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(12419..12763)
                     /locus_tag="IW774_001103"
     CDS             complement(12419..12763)
                     /locus_tag="IW774_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153460.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLGPRTERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(12783..13340)
                     /locus_tag="IW774_001104"
     CDS             complement(12783..13340)
                     /locus_tag="IW774_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153461.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIRVIKKLCTIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFHKREKNQMSAEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEKLMMDFSDLYRGYECSVIQER"
     gene            complement(13420..13920)
                     /locus_tag="IW774_001105"
     CDS             complement(13420..13920)
                     /locus_tag="IW774_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153462.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPKEYPIEKRELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPINFLKKC
                     HLRILP"
     gene            complement(13921..14466)
                     /locus_tag="IW774_001106"
     CDS             complement(13921..14466)
                     /locus_tag="IW774_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153463.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGTLKFIRFYRFSLPVPAILAVK
                     KGCDKMKIYENCVAALSNEKPLVEEISLATVTWEMNRQPTEEQTYLSLEEIELELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(14463..15719)
                     /locus_tag="IW774_001107"
     CDS             complement(14463..15719)
                     /locus_tag="IW774_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1153464.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKKKIPEDFEKRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKIVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKQTVTQNVLVQFSGQGCREYEEVMREQRTD
                     WRTWLYSIWKNQGKLEAGYKRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKKFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISEIEAL
                     TKYGIYNRYELRLANEKATQAVEAFILDHSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIENAELNDEQQSYLEWMGTSGGKIA"
     gene            complement(16082..16396)
                     /locus_tag="IW774_001108"
     CDS             complement(16082..16396)
                     /locus_tag="IW774_001108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153465.1"
                     /translation="MGEVLYRVSSAAGEISPDFAVRRLYEWINKVEYYTKGSYVFRRI
                     ERETLFVTRNQIVLTKEDILRFRQVYRLCKEKNIQLRLAILQCFAPEQYKELQEKEDS
                     LV"
     gene            complement(16523..17716)
                     /locus_tag="IW774_001109"
                     /pseudo
     CDS             complement(16523..17716)
                     /locus_tag="IW774_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            complement(17906..18262)
                     /locus_tag="IW774_001110"
     CDS             complement(17906..18262)
                     /locus_tag="IW774_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153466.1"
                     /translation="MNLDKTNQTLREIMVCCQNIQQLIEYFGDNPTIFIQTNEYICAV
                     IGQCMFLRDLVRFSLPYQWKQDHQEVVWIRVVRICNMCAHPYKNRLNLFRLWFFIKCD
                     IPVLVAFCQKFLAQEK"
     gene            complement(18328..18780)
                     /locus_tag="IW774_001111"
     CDS             complement(18328..18780)
                     /locus_tag="IW774_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153467.1"
                     /translation="MGLKFPNNYDITEALDETCIGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNSNPKQSQEKGLDQKQPSADSKEKEKK"
     gene            complement(18782..19147)
                     /locus_tag="IW774_001112"
     CDS             complement(18782..19147)
                     /locus_tag="IW774_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153468.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            complement(19164..19418)
                     /locus_tag="IW774_001113"
     CDS             complement(19164..19418)
                     /locus_tag="IW774_001113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153469.1"
                     /translation="MEKELLLGFYRKENNGGKKNMEKKNINICFLSGKECTYEECTDI
                     SVHERDGMLIVDMSPSDEPFVVKHYFPLCNIESFRIDCEF"
     gene            complement(19375..19620)
                     /locus_tag="IW774_001114"
     CDS             complement(19375..19620)
                     /locus_tag="IW774_001114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153470.1"
                     /translation="MEEIFFCMMVEFLFVSIGFLIMGEAIMIYLFPFDSKDECIFSSI
                     IIFFILACVWILAREIYHQFVLEDGKGIITWFLQKRK"
BASE COUNT         6157 a         4247 c         2769 g         6762 t
ORIGIN      
        1 cttaccttca tttacatcat tcagcggttt ctcattttct tttattctat tatttcattt
       61 tctaatagac ttggcttcat ttcgcaatac ttttttacaa taggcatcaa attggtgttg
      121 tttagatatt tagttgtagt tttgtgacat gataagtatg ctcctttttg tcttttccac
      181 taatacaacg atcaaagggt gcttttttca aaaaactaga aaaatcaaaa taagattcgt
      241 attttgattc tatatatcct gtttcaacta catcttatat ttcataatag aataaatagc
      301 cttttgcata gctgcttaac ttataagaaa ttgcaattta cttcttcaaa gtgaaaacga
      361 atatgagtaa aataaaatta ttcttcttac agataattaa aaatagcgcg taacaaaaat
      421 cggcattcaa tcacataatt aggattgatg ccgattactt atattctacg tatagtttta
      481 gtattctttt tttatcacca gtaaccaatt gagaacagtc ctaaagcaga atagtctttt
      541 atctcaaaac gcttaatttc aggcttagga atatgaataa tcttttgagt atattctttc
      601 tcttttaatc ttactgaaga aataccactg cctatctttt ttacaaatat ttcatttggt
      661 gtacagacta ataaaacaat atttgtaatc aatccttgca ctaaagaatt aaaaggaaga
      721 ctatttaaaa cagggtgtgc ataactataa catgtgaagt agttattttg aactttaact
      781 tttaaaaact ttaaaaataa tcttatttgt tcttctaaac aatcatctat actcatataa
      841 tcactccact aattacttcc cccaacaaga aatagcatta atagctatac cagctgcaat
      901 agttgcagca ttgaattttg caaattgtgc aggagttaaa cttctttttg ctacatctaa
      961 aagtatttta acagcataat cataattttt cccttgcaac gcattaatta aagtttgcat
     1021 tagattttca cgattaagta tatcccaaac aacacaccaa atggtaatga gttcataaca
     1081 acacatgcag catacttttg aagatcacgt ttttgacgct ttcctgttgg ctgcacaaaa
     1141 ttttctaaaa taaatttccc tgctatacct tcagagccat ctaattcagc ttttttctgt
     1201 atagcttcta aatcagtaac tatatattga tgtgtttctg tatcaacata accagcttct
     1261 tctaaataga atttcaaact ctctactgcc tgttgtcttt gtgtttctat atcaggaact
     1321 tcattcactt tttcagcagc ataaacactg gttccggcac cgactaacgt acttaatcca
     1381 attaacatta cacctaataa tgaaaaaaac ttaaaaaaac gactcatttt tatccctcct
     1441 gaaaagtatt gttgattatc aatttttatc cttttaagct gttcaataag acgtttttac
     1501 gttccttccg tttctatttt attctttctg tcttataaca gcttttttca agtaaagaac
     1561 gatataattc ttcttaaaaa acagtctatt ttttcggtat ataataaaat tgtattgtta
     1621 cctacatagc gaaacagtac caacatcatt caaatgaaga tagcaaattt gcttcttcct
     1681 ttttcttaag tgactattta aataaccaga aaaaaactaa aatcacactg atatcccaaa
     1741 taaattgaaa taaaggaaaa agtttttttc taccatgata gtttttcaaa aaatagaaac
     1801 tcgtactatt taaaagtaaa ataactagca tcgtaaagac tactgattta tctaaacttc
     1861 tataaaaaag tgggctaaat aataaagtaa ttaaaaaatg aactatttga tattgttttt
     1921 tttcatatta acagatcgca gctcctgcga ttccataggc tgttcctaca gcaaatggaa
     1981 taacggctgc acctactccc gtaaggctta aacctagagc agcagctgta taaatcccag
     2041 aatgtagaag tccagcccca gtgaaagccc aactgcaggc acttcgttca ccagttttat
     2101 tatttgtttt ttgatctttc atataattct caacttcatg aagctgatcg gttagccatc
     2161 catggccatt ttttttgaat agaacgatta aattcattta gatactgttg gatttcagaa
     2221 actgtaattt cagaattaat tctatctaaa cttgatttta aatcactctc atcaataata
     2281 tatactaatc gatttccttt agaatcttta tgtgtggcta aattattttg aatgatattt
     2341 atttcagttt cttgttgttt tactaaaaaa ttatttgttt cagaagcatc gacacttatt
     2401 ccagcgctaa caaaagtact taaacctatt aagaaaacac ctaaaatacc aaatatttta
     2461 actattcttt tcataatttt tcctacttaa aaataagtta tttttaaaat atcttgatta
     2521 tgaattcatc ccccaaaggc cttttaagat tgatacagca gtccgttcac ttcttttaga
     2581 cttgtcatat aagtctttta aatgatccca taccagacta gtttgtctat ctagcttcaa
     2641 tccattaaac gctaatgaac ggttcatttt ttctattact ttagataaag ctacagaaat
     2701 ttttatacct tcacaacgcg catcttggat ttcaataata gtatcatcaa ttagtttttg
     2761 aatttctttc ctatcaacat attcaatctt agaaagagaa tcttttaaag ctactaatga
     2821 ttcaacgatt tcttttttct cttcagacat tctcttcctc cttctagtga cccgtaaagc
     2881 catgtagagc agagtccatt gaaccagtaa ttacactatc tacaacgcca ttccaattta
     2941 ctctacattt tccatttttc agatttgtcg gtccatacgg atagactttt tgacattcaa
     3001 tgttcggatt ataccagata ggattatacc attcagccgc agaagcagat tcagtcgata
     3061 caaacaatcc cacaccaaca aaaccaatac acatcacact atagccaatt aattttttca
     3121 tctcgtatcc atctcctaaa aaatagaata gttcttgtag ttattcataa gacatatttt
     3181 ttccgttttt gttttatcct ttctgtccta taacaacttt tttcaagtaa agaatgacat
     3241 acttcttctt aaaaaaacag tctgtttttt tctatttttt cggtgcataa taaatatgag
     3301 tgttcatttt ttattttaat acacacttcg tacataataa atcttgatcg catcaatctt
     3361 ttgagcttct tgatctactt tcaattgaag taatacaaat tgagtgacct cattcgttgt
     3421 ttcttctcca aataacaaat attgatccgg attctctata tcttgtgtca cctggtgaag
     3481 tttcccagtt gttttcattt gtacatgcgg gtctacatca atactattgt ctttaataca
     3541 acgatccgtc atatagtccc tcacagcttg attgcgttta taaacatctg tgaaattact
     3601 ccattttcta gcaaaatctt ttaagaaatt ttttgtcatt tcatctattt gttgcgctgt
     3661 ctcgtcttct aatgaaccaa tttgactgtt actttcagta ctttgaagag ttccacttgt
     3721 ttcttttact gcttcgattg tttcttctgt cgttgtcaat tgtgtttttt gtttttcaga
     3781 agaaaaccaa tgacaaccag taagacctat caaacataac caactaaata ctaaaattat
     3841 tccttttttc acgcttctag acactgcctt tcttatttca ccttacgaac cagactacga
     3901 atttttgttt gattgtacgt gcgttgatac cgagccataa tccgtccttg ctcggcattc
     3961 tgttccacac tagagaaatg ttgtccgcca tctgaaacat ctgttataat tccggtgtga
     4021 ccatagattc caggagacaa tgcaccacct gcttgccaat tcaccacatc tcctgcttgt
     4081 aaatcacttg gttttgggtt catgatcact tgccaaccat aggctgtcca atcataatct
     4141 tcccctatta acatcgcaaa catgtgtcca gaacccatta actgtgggcc acctagttgg
     4201 gatacatacc acgccgtgcc accataacac tctccattat ttaccgatcg tccaatgatc
     4261 cgatctaaaa ctttcgcatt acctttactt ttccctgcaa caagtgacga tttttttgtt
     4321 ggtaggctat tccaatgttt gatcgcaaaa gtatcatatt ctgttaagtt ataagacttt
     4381 atgatactca tcgctccttg gtaatacgga ctgccttcgg taatacctgc cgccgcatag
     4441 cctccacgac ctagaatcaa taaacaactc ccaggatctt tattgcccac acattgatta
     4501 aagttgcttt gactcgccac caaaaagccg taatagtcat aacaatcttt caggttcgga
     4561 aacttcatat agaagccacc ttcagaacca ccaatccccc gtgcagaacc ttgtggatag
     4621 ggacaaccag gaaaccaggt aatcccaaag aagtttgtat cgtttcttcc agattgtgat
     4681 tgtccataag tcgattcata acctaattga gaaagaatca cacttggtaa taactgatac
     4741 tttaccgctg caccgactgc tgcttgcgca tgttcatctg tgatcggacc acgtccttta
     4801 atctctgaaa aaatcggtaa tccttcggta tattcccaat taaaagcgac agctagacct
     4861 gtagcctctc ctccatcctc ggaaagttct cctgaaaaaa agaatggcaa tagaaaaaag
     4921 ggaagaataa agaaaagcag acaacctaga caccctttct tcatttcttc ctcatccttt
     4981 cttcgataat ggtttttgaa ctgttcgtcc ttttggtaaa gtaaaatcgg ttcctggttt
     5041 tgcgtccaaa atcggcaatg tttcattttt cttttctatt tttttcgatt gctctactgg
     5101 attattaaaa gaagaaaatg caggttgtaa ggattttggt acggaaatcg acggtttact
     5161 ttcagacgaa cgttgtatct gtcgttgcac agaacgattt cctgatagag tggctaccga
     5221 cgacttagga acagagtatt gattcaataa ctgctgcatt gctggataac tcccattttt
     5281 cgcctgattt ttcacttggt gaatctgttg tgaaagagaa ccagcagctg ttaattgtgg
     5341 cacgggtact tttccagcag gcaacggaat cgattgacca acaaaagcga tacctttatt
     5401 taacaatggt tttctttgat tattcccttg ttgcttactc gattgtgcca acgattgtac
     5461 ctgttgtttc ggtcttactg aattcatgga cgaatttgga atcaaaccat ttgaaagacc
     5521 agaattccac tctgtttttg atttggtaga agacatttca ggctctaccc catgataacg
     5581 aacatgacga ccactataag gcaagtcttg atttgaacgc ccacctagtt caaagggatt
     5641 aatcacttgc ccccctttta ccgattcttg tggtgtacgt ttttgttgaa acttttgaaa
     5701 tccatgtatc gctgcagaag cgagtggatt cgttgctcgg tacacattcc ctgctaatcc
     5761 tgcacctttg attgccaaat tttgcccagc agttgatagt tgaccaggca gttgtttcat
     5821 ttgagacaaa gaatgacttg ctatgttttt cgtgttattt atggtttgat ctgcagctcc
     5881 taaccctagt ttctttaata aatctttccg ttttacaatg agtaaaattg ataggagtgc
     5941 tttgacaaac atatttccta aaaaaccaaa aacagtattc attagaatta aatcgtctac
     6001 tatgttatag atcaaaaatg ccaccaaaat aatcacactg taaaaagatt tttgaaacat
     6061 caagctcaaa aaggacttta aggtattcat gatcatataa tcaaatgctg gaacaaaaga
     6121 agcgagtaat gaaaaaggaa tcacaagtaa taaacacaac accaacattt gaaagataaa
     6181 acgaatcatg ccaatcacta caagtggtaa tccaatactt agattaagca atggcaccat
     6241 agaaccaacg actgctttat aaacagcttt agttggttga atatagacac gataagcctt
     6301 ctttttccct ctatcttctc ctgcagcagt ggtcactttt gtctcaatat cagataattc
     6361 ttcacttgac gcttccttgg ctagaaattc ttgtggttca atctctgctt gatacaattc
     6421 ttctaaattt gttgtcccat aatttaacaa tagatacgga tcaacaacag ccgctttata
     6481 ataaagttgg cgcacttttt gaatggcttc cttttgtgtc gttggcacta aaacaccatt
     6541 gacatctttt gtcgccaatt catctagact atttgtagca gaaaaaataa agccttctac
     6601 ctccacggac aaatcattta accctttagt aaaagcttct cctttactag gtacgttact
     6661 accatagcca aaccaaacca ttgaaaaaat gacaacaaag agaaatcgaa taaacaccat
     6721 ttttgcgtaa tgtttccctc taaatatacg aagagtaaat agatataaca catacaagac
     6781 gacaatcccc gcaccaatag gatcgtatat tttcgaataa atagtatccg aaaaagtgaa
     6841 aaactggtga attaactcgt tcattgcagc tccttcatag agcttctcaa tgacaaaatc
     6901 aaggccttgc caaatcaatt tacttaaact aaagaacata ttcgtcatca tttttagaaa
     6961 atctgtgcca tttgctccaa ataacatttt gcctaatgaa aattcactat cagataaata
     7021 agaagaaaag gtatcaatat gaaatttccc gatctcatag atcgattgat ccacctgttt
     7081 ttctaccgca tataccaatc caccaacaaa aaacagtccg aatccaaccg ttaaaaaaag
     7141 ccaaacgcac catttctttt ccttacgctt tatataagcc attgtttcac ctcgtttcta
     7201 ttgcatgatt aacgtatcct gttcttctat ttgagagtga cttgtttttt ccgttgtttg
     7261 taatgcagcg gtcatttctt caaacaaact gtgtacattg agttttccta cacgtccata
     7321 gggatctaac atccaacact gtcctttaat catatggctc atttcagata agttcgcttc
     7381 atttacttcc aaacgtaaat gagtcaaaat cttttctcgc tcactgtctt catcaaaagc
     7441 gaagacaaca ccaaaattcc ctttttcttt atctgaacct aagtcgccaa taaattgtgt
     7501 atctagcact aattgattca tctgtgaacg ccccacacgc gccattgctg caataatact
     7561 acgtcccgcg ttcgaattat taaataccca tgcttctgta aaataaatcg ctgttttctt
     7621 actactatct cgttttccaa acatttcaca aaaacgacct aaactaatca tcaaacacaa
     7681 agactttcga tccgcatctg tatatagttc tggtcggaca ttgtcatttg gtaatttcaa
     7741 atcttggatc tctaaaatcg ttgttttggc attaaagtct aatcccgggt tttctccata
     7801 agaaaatcct aaacgtaaaa tcgaatcttc taccgtcaag cgtaaaaact caccatattc
     7861 tcgaacctct ttttgttcca tttgttctaa atactcgacc acatgcatca agccaacttg
     7921 ttgtccggct tctttttgaa ctaaaacttc ttggattccc tttaataccg ccccttttaa
     7981 aaggtgattg gtacctaacg gacttaactc atcaatcata tcctgggcta cttgtttcgc
     8041 atctactcgg gataaacaaa caattggatc taatacgcca taattacggg gatttgtagc
     8101 atctaacgtg acataactca ttttttgcaa atgttccaca aacaaagggt aatgtttctg
     8161 ataatacggg tcttgaatga gtttcgtaaa ccatttgcgc ttttctcctt tgggatccac
     8221 aaataaactt tgtacattca taaacgaact atacaaaaag agcaatccaa ctaaaaaggt
     8281 cttcccttta ccggtttttc cagttactgc tacatgaggc gcatcaaaca acgcaccttt
     8341 gattccttcc gcaatagcta gaagattcaa aaagacaaaa cgatttgacg cataaattaa
     8401 ttcttctaat ggtgtttttt ctcctcgaga accaccatcc cctaatgtat cttgccgacc
     8461 aatataccag ccggattcca tacctaattc tgatactgct gaaaacaacc attcgccaat
     8521 tgcttctaca gttgttcggt gcttccaatg caactgtcga cccaatggtt gccctaataa
     8581 acatttatag aacaattctc gttgatcatt ttttgcacgg tggatatgga tagtcgtatg
     8641 aagctgttga ataatcgttg cactattttg acgaacttct tcaaccgttt gaccgtatac
     8701 aataaaacat gcgatccaat cgacgacagg ttggccatta tctactcgtt gctttaaatg
     8761 ttttactaat ccccgaacag ttaataagcg ttcgctaggt tcttcatcgt tttgaaccaa
     8821 ttccttatgt tctgttttga accgttccga tagccaattt agttttaaac caatacctcc
     8881 ctgtcctttc aagcgtggat agtgaatttt cattcgtaat tctgctggga aagaaagatc
     8941 ctgtgcaaat tgaaatagat cacagtttct catattttca ggtgtttctc caataggtaa
     9001 caacgctaaa taactagtcc ccaactcatc ttctaattga agaaggcctt tatgttcctt
     9061 cggcatacgt acatacgttc cttgattgtc tttcagtact tcccaattat tttgttcttc
     9121 tactttatga gccattcctc gaatatattg cagacgattg agatacaaca gctctttttt
     9181 tactaaggga cgaatagaaa agctataaag taattgtgtc acttctttta cataaggatc
     9241 actttcagaa aacaaagttt ctaaatcatc tacttctaat ccaaataagc gtgctaaact
     9301 tcctgctaca tctaaccaga ttagatttag tacatcataa aaactctcca cggttgcttt
     9361 ttctttgact ttgattccta aaataaagga aggacgatag acttgacctt ctcgttccaa
     9421 caatgtcact gtttcttttg cataatacgc accaattgct tgactatcgg gagaaaatcc
     9481 ctttcctaat gtgtcaaatt tcgtccgtaa attcattgtt cgtggataca tcaccaactc
     9541 cacatcttca taatctgcta aaagtaacag taaacgagcc atcatttctt ttatttcctg
     9601 tgtctttttt ggtgtgtttt ttgaaatggt tcgtggaaaa acatgatagt aggcccagat
     9661 acaatcctcg ccatttaaca aacaattttt ctttaatgct ttcactggtt cttttacaag
     9721 taccaataga tctcacctcc caattaaaaa aaggaacaac cagttgattg tccctttttc
     9781 taatcctcta aataaacata cttattcgca aaaagtaatt cttccaatgc attatttgat
     9841 accttgtccc agttataata cacacaatca ccattcatca tacgcactac ataacaaaac
     9901 caatcccata aataaaaatg cacttttttt ccatcaggtt ttagttttgc cagtgctaac
     9961 acacataaat agggtagccc tataaaaaaa acaacccctg ctggtggaaa aaaagaaata
    10021 acccaaccaa taaatgaacg taaaaataaa tattcaaaga ttacgaccaa taaaaaagtt
    10081 gctgcattga ctaatgcaac tggaaatgga agacgccaat tgcttgtaat ctgttgtacg
    10141 cgataaggct ccgcataaac aatcgaataa tcgtaaggtt cccaatccac ttcactatcc
    10201 tcctaatcca taaataattt ttcaatgatt ttaccaaacg catcaaatac acgcgttgga
    10261 tcatttaaga agaaatagac tgcggcacct gctgcgatag atattaatgc atttcctact
    10321 tttgatttca taaaatttcg caatcccaga taaacaacca ccattattaa aaaatagcca
    10381 ccttgcgtta atataaaatc tacaattccc ttcagtgtca tttccgttcg tctcctttcc
    10441 tatccaacca attatgtgtg agtttttcaa cataatactg atttgttttt ttcgtaataa
    10501 caagcgtcat ttgttctttt tgtaaaagtg acgtatcacc atctcgaaag acgactgttg
    10561 cttttacaat aaaagttttt ttcgtttctt tataacgcaa tactttcatc tctacttgat
    10621 caactttagc tgtctgattg agtccttcag gttctttcat catgtatgac atatcttttg
    10681 cattgttttt cgcatactta tctaagaaat cttccacaaa tccttccaca tctttcgctt
    10741 ccgacgcatc aaccgtttcg tataatgtgt catcataagt aatcattcgt tgtttttctg
    10801 tattctttgg tagtgccgta aaataaggat aagcaataat acgacagccc ttgtttgaaa
    10861 tagaaaaggg aagattgagt acttgtgtga ttgtcacact tgtttctttc ttattctctg
    10921 gatccatcga gcgaatatca taagttactt tataactagc tgttggaatg gtttcaattt
    10981 cttttaatcc aataaacgaa gcagcagtta attttcgaat acctgaaaca cttcctggtt
    11041 cttgaataaa gttttgtgca taaaactctt tttttaatat ttcttgacgt tgttgttctt
    11101 gttcatgatt atcgaatgta ttcatataca aagcaacaaa acgattcata aaggattcaa
    11161 tttcaggtgt gtacgttaat tctgactgac tttgtacttc tttcttttgt aattgttgga
    11221 tttcatcttt caaaaacgac acttgaacct tcgttttcat ggattgaaaa aatgccaagc
    11281 cccctgaaaa acaaagaaat aaaaccaaac accaaatcca tttcactgct ttttttgaat
    11341 ggattttcct ttgattcggt actttctctg tataaacttt ttctttccgc cgtagttgtc
    11401 gttttttcat aagctactcc ttctagtatg tttttactag tacgtacgat tgacaacgat
    11461 aagcttgttt ttctgaaaaa tagtccattc aattaccctc attttctatt ccttttctct
    11521 ttcttttata tccaacaaat ccagcaatac ttgtcaacac tcctcctaga acagaaagta
    11581 caacatgtat ttcttcacca gtctttggta agacttttgt ttttccatct gctgttttga
    11641 cttcaatatt gccacttgga agtttcttta caggaatcga caaatccttt accaaattcc
    11701 cttcactatc ttggattaat ggaattccat cttttacacc aacaattgtt tgtccacctt
    11761 ctgcttttag tggagttgtt ggtgttggaa tatgggtcaa ttcctctaca ttactagtga
    11821 caatcggtac actcattcct tgagaattat tcgtcgtaat ttcaccgttt ggggttaccg
    11881 atactgtgac ttcttttgct ggaacaatag gtttattttc ttttggaatt gcttctgatt
    11941 tttcagcagg ttgtttcggt tcttcttttg gttcttcact ggtaaacgac atcgttggct
    12001 gttccgtact tgacgaatcg gtattcgttt cagaagaact ggttgttcct gattgttctg
    12061 gagttgtgac tatacttgaa tccgttgttg ttgaactgtg cacaccttct tcttcaattg
    12121 gatcagtcgg caaagttgat tcaggaatct ctggttctgg tgccactggc tctttttcag
    12181 gtttctctgg ctgcaaatgt tcaggttctg ctggtttttc cgttgttcct ccatttagct
    12241 ctgtctcaac atctgataat ccttcccctg gcaatgtttc tacaatttct gacttaggtg
    12301 tttctacagt ttcatctgca taaaccaatg aactccctaa ttgaaacgaa aataatgaaa
    12361 ttcctagtgt tgttacgact gctaatgttg tttttttcat gattattcct ccaatttttt
    12421 atgcaattcg tgttttgttc atagacgccg cactaatatt atctgaacga taaaagaact
    12481 cccgtccgtt ttcttctaac ccaacatatg gattattttt gaataattga atcaatagtt
    12541 cactggtgtt gtattttgga tagtttgcaa aaaaataata ctgtgcttgt cgcccatccg
    12601 aaaattcaac aagtaattca taacgatacc gcttcaatcc ttttcgttgt tttaaaggat
    12661 ctctatacca acttgtccat cgttccgttc gtggaccgag atagacacga tacacataga
    12721 acaatgccat accacatacc accaaaaata ataatcgcca cataaaaatt cccccttttc
    12781 ttctaccttt cttgaatcac cgaacattca taccctcggt agagatccga aaaatccatc
    12841 attaattttt cgtataaacg ttgttgcaaa tagcgattgt ttcgaataca taattcttca
    12901 tagccatcaa tcacttgatc ttctttctca tagcatcttg tagctctatg acgctcagct
    12961 tgcatgacat aaccaaactc tgcaccatat cgttctaccg ttaataactg ttgactacgc
    13021 aataatgcaa tataacaatc acaaaaatgt tcagccgaca tttgattttt ctcacgtttg
    13081 tgaaataggt attctttaga aagcacatta ttcgttaaac aatctgataa atagcgataa
    13141 tctggcggtc ggttattttc atctgtgaat ttttcccata ataaactgat ttttattcgt
    13201 tcagcggcta gatagtcttg ttcgcgtgga taaaattcat caatcgtaca aagctttttt
    13261 atcacccgaa taaacaaatc catataatgc gaaacgaact cttccttttc aattggctct
    13321 cctaaaaatt ccagtgacat accatcttct ccttccttaa aaaataaaaa agcagaaacc
    13381 gatttggctt ctacttttct ttccttttat agctcttttt tacggaagaa tccgcaaatg
    13441 acattttttc aaaaaattaa tcggcacaag ttcacgaaaa cttggagcaa ttttagatag
    13501 aatcacttcc ccatcttgta aaatacaatc ataaaaatca tcagttactt gatacttacg
    13561 cttcttacaa taattgataa aatagtagcg atcattttct atttccattc gttgttctac
    13621 taagcgcata cgaacatcat catagaaata agccacaaat cgcccttcaa gttcacgttt
    13681 ttcaatggga tactcttttg gtaacaatct attttttcgt cgtttataca ttgctggata
    13741 ccttagaaga agttcatcca aacgtctgtc agtatatggc aactgacgaa tcaatgattc
    13801 caatcgttct ttatacagat agaataattg gcgcaagcaa tagtgtactg cttctggatt
    13861 ttctaatact gttggatcac gcgaccaata cgcaccaaca taaatcataa tttttttcat
    13921 ttagacaatc acaccagcaa tactaatcac cgatccacaa taggtagaac aagtggcata
    13981 gcggccgatt tcctctaatt ccagctcaat ctcttctaaa gaaagatacg tctgttcttc
    14041 tgttggctgg cgattcattt cccaagtaac agttgccaac gagatttctt ccacaagagg
    14101 tttttcgttg ctaagtgctg ccacacagtt ttcgtatatc ttcattttat cgcagccttt
    14161 ttttaccgct aaaatcgcag gtaccggcaa tgaaaatcga taaaagcgaa tgaattttag
    14221 cgttcctttt cttcggctaa attccaactc ttcgagttct tgttcgtacc ccttacgaac
    14281 ttctaataag cgtgcaattt cttgccagtt ttctttttcc tgataacgtc cagcctttac
    14341 cgcttgttca atataaggaa ctcgaaaacg aaaataagac aaatcaacca tcttttcatt
    14401 taatgcttca attgcagcct ttaaatccaa aagacgcagc cattctgttg tcgtatatgc
    14461 attcatgcaa ttttccctcc tgacgttccc atccattcta gatagctttg ttgctcgtca
    14521 tttagttcag cattctcaat tccttcttgt acaaagtctc ttcctaaaat acgatctgct
    14581 tcttgtaata actttaatgt cggagctact tgtttcaaaa accagcgttc tgaacgatct
    14641 aatgagaatt tttctggctt tacggtaaaa tccaattctt ccatcgaacg aaacaatttt
    14701 gcccatcgtt catcaacaat tcgtgcatca ctttccagtc gatcccaaac aataagtttt
    14761 gcattaatta aaccaacgcc aatctctcct aaacgtttag gggaatgatc caaaataaac
    14821 gcttctactg cttgtgttgc tttttcatta gctaggcgta actcatatcg attataaatg
    14881 ccatatttcg ttaatgcttc tatttctgaa attttctcac gattcgcaat ttcaaaacgt
    14941 ttttcgtaga aattgaaaaa caatggactc tttcgcgatc caaaatacaa ggataaccct
    15001 tcgcttcgga aataaccatc atctaaaaaa caaccgccaa tattctgaaa tttcttaaag
    15061 tccatttcta ataaacctgc tttttgtttt tctagtaatt caaagagatc aaaatgttct
    15121 tctgtttcca atccccaaag ttcatctaac gcaatatcga atcgactaca ttgtactcgt
    15181 ttataaccag cttccaactt tccttgattt ttccaaatgg aatatagcca tgttcgccaa
    15241 tctgttcgtt gttcacgcat gacttcctca tattctcggc aaccttgacc cgaaaactgt
    15301 acaagtacat tttgtgttac agtttgtttt gaatgccaga atacccagat atttccacga
    15361 acagaaagac aatcaaaaag atacaagccc gtcggacgtg tttcaaattc tgttagatcc
    15421 attcgtaaca cacgttgaat cactattttg tagtctgtcc caaaaaagga gagacgcaaa
    15481 tagtcgatca ttgctcgaag atctattccg cttttcatat acaatcgaaa gcgttccttg
    15541 attcgttttt cgaaatcctc tgggattttc ttttttcctt gttccatctt ctttatataa
    15601 tctaccgagc atcccaataa ctgtgcaaaa cgtttttgag agagtaattt tttccctcca
    15661 cctgaagcgt gaatagcact caattttttt cgataatttc gcaattcaat aggtgtcata
    15721 ttctacctcc tttataaaat aacaaatttc gattttctta atctgctaga aatcccgata
    15781 taacaggtgg taccccctaa tttttctaaa aaggagtact ccttctaacc ttagctaaag
    15841 ccttgatata actgatttaa atacactttt ttatttgtgt tatgttattt ctgcccccca
    15901 tattatatat cggggggctt tacacaaaga ggccgtgcgc aagcgcacga cctctttgtg
    15961 ggcacacatg ccaagcatgt gctgccacga ctggtctcgg tggcttcgcc gacacaagcc
    16021 gcggcagcac atactcggac aatgtgttct cccatagtcc ctatgaaagc caccccgata
    16081 tctaaaccaa tgaatcttct ttttcttgta attctttata ctgttcagga gcaaaacact
    16141 gtaaaatcgc taaacgtaac tgtatgtttt tctctttgca taaccgatat acctgacgaa
    16201 aacgcaatat atcctctttt gtcaaaacaa tctgatttct cgtaacaaac aatgtctcgc
    16261 gctcaattcg tcggaaaaca taagaacctt ttgtgtagta ttctactttg ttaatccact
    16321 cgtataatct tcgaaccgca aaatcaggtg aaatttcacc cgcagctgaa gaaacacgat
    16381 acaaaacttc acccatactt caccacttcc ttctaaatta taaaagtgta taactttcct
    16441 catctacccc atttgttttc gtatttcctt ttaacgattg cgcttgacga tcaatttgtt
    16501 cttcgtaact agtaaacgcc tcttctttca gtacagaaaa atcttgcaca atcattgtgt
    16561 caaatttttt aaattcctca ataaagtgat agtctttgta aacaaatggc gcaaagaatt
    16621 ctacaggcac atcaaaacca ggaaaagcat acccacgacc aggttgatct ttgttataaa
    16681 acggcttatt cttaaaatcc tctccaaaag tcattaaata gccaatggga gataaacggc
    16741 caacagaaat acggaacgtt aaactatctc gaacaccact tggaaaatat tccgcatctg
    16801 gtcgctgcat cgctaaaata acatacatgc ctgcttgacg accttttaat actaatggct
    16861 gaattaattt atcaactttg tttctcgttt ccatatccaa cgaaccatag aaagctgccc
    16921 attcatcaaa aaagataaaa tgcggcggta tcccataata agcataattt ttccctggtt
    16981 catacttagg ctctttcatc gttttcatat aggtatatcg ctgattcatt tcttgtaacg
    17041 catttcgtaa gcaaataatc atttcttcat tcgttttgta aaacacatgt cctttgaata
    17101 catcaatatc acctaatgcc gctaaatctg ccgctttccc atcgcaaata tctactgtcc
    17161 ctacatctaa acaagctttg atcaatgaat acaataaata ggtttttccg ccaccaatcg
    17221 caccagaaat taatagattt ggcgattgat catataccca atccaatgtt ttcattaaat
    17281 gaatacgtcc attttctgct atacattgat caataggaat ccgaccagaa tcggaaagaa
    17341 atacataatt gattcgggct ttttctcgtt cttcacgaat taaatcacgc aaaaatactt
    17401 cggataataa gctacccatt tttaaaaatc gttcgtggaa acggccacca tctaaaggaa
    17461 aagaaaccac taattcttgt ttcttttgat aaaaataaac ttttggtaat ctcatttttt
    17521 ccttcgtttg tttctcacca acttgcttct ttgtataaac aaaccgattg tcaattaaca
    17581 ttcgagcaag taactgacgc caataaacag aagcaaaata tccttccttt agtcgcgacc
    17641 aacgcatcaa acctaaaatt aatccactaa ccagcaacaa atacatcaca atactgattc
    17701 caggaataag atacatttgc caattcgaat acgtatttaa acgaggataa aatactttcc
    17761 aactaaacca acttaataga atcaaaaatg gtaacagaaa gaaaccataa taacaaaacc
    17821 ataaataacg attgaaataa cgaattcgtc gaccacgata cttaaaaaaa tgaggttcca
    17881 attgtcacat tcctcctttc tgtttttact tctcctgtgc taaaaatttt tgacaaaaag
    17941 caactaatac tggaatatca catttaataa aaaaccataa tcgaaaaaga tttaatcgat
    18001 ttttatatgg gtgtgcacac atattacata ttcgtactac acgaatccaa accacttcct
    18061 gatggtcttg tttccactga tacggtaatg aaaaacgaac aagatcacgt aaaaacatac
    18121 actgcccaat aaccgcacaa atatactcat ttgtttgtat aaaaatcgtt ggattgtctc
    18181 caaaatactc aatcaattgt tggatatttt gacaacaaac cataatctct cgaagtgttt
    18241 gattggtttt gtccagattc aaaatctccc cttccttcta ccttaaatag aaagagtagg
    18301 aagacttcct cctactcttt aaaaaactta ttttttctct ttttccttag aatcagcact
    18361 tggttgtttt tgatctaaac ctttttcttg tgattgtttc ggattagaat tatttgttgg
    18421 agcttctact ttctctaatc ctttcgcata aatcactaaa tctgaatatt cacgattgcc
    18481 atcttgtgct ggaatatata ccacatgata ttctggatca attaatttca caggatcacg
    18541 tcgtttaaaa ccagtcaaat ctacatctgc tggaaattta actcgtcgtg caatctttgg
    18601 ttcatctgga tttccaaaaa ctaattgtgc tcgctgtgat cgaatttctc ccgtataatc
    18661 gcctgtttca ttatcaaata cacgatcgga ctcttctaat gcgccaaagc gtacggctcc
    18721 tatacaagtt tcatctaatg cttctgtaat atcataatta tttggaaatt ttaatcccat
    18781 cttacaaaac ctcctcaatt tttacaccag ttaaaaagac accgacatct tgatagccag
    18841 ttgttcgacg ctctgtttgt cccatatcat ttgtttcacg actattcact ctttctttac
    18901 gaaaaacaaa ataagcttct tcaaatgaaa tacgtacctc tttttctggt aatcgtaatc
    18961 ttttatcctc ttcagctgtg agataaacaa aaaaagcagg ctgttcttct cgcgaacatt
    19021 ctgcttgtaa ttgaacatat cgcccagaca caccattaat ttcctttggt ggattaggtt
    19081 cccgtgaacc caataaaaat acctgaccta aaaactcttt cttcatacgc aatgcttttt
    19141 ctgccatatc atttttcctc ctcttaaaat tcacaatcaa ttctaaacga ctctatatta
    19201 cacaatggaa agtaatgttt aacaacaaat ggctcatcac taggagacat atctactatt
    19261 aacatcccgt ctcgttcatg cacagaaata tcagtacact cctcatacgt acattctttt
    19321 ccactcaaaa aacaaatatt tatatttttc ttttccatat tcttttttcc tccattattt
    19381 tcttttctgt aaaaaccaag taataattcc ttttccatct tccaacacaa actgatgata
    19441 tatttcccta gcaagaatcc acacacatgc taaaataaag aaaataataa tactactaaa
    19501 gatgcactca tccttgctgt caaacggaaa aagatatatc ataattgcct cccccataat
    19561 taagaaacca attgatacaa acaaaaactc taccatcata caaaagaata tttcttccaa
    19621 ctcttttaca tcctttctaa ctttttataa aaaaggagat tatttttata aacactttta
    19681 ttcaacaact attttcaaat gaattacaca ttatcatctc aagtatagtt tcaatcgtca
    19741 ctattgtttg gtccatttct agcttttatt taaaacatcg ctatacaaga aaaaagaaaa
    19801 gcactgaaac cctaacaaaa gaaatgttta ttccatttaa atcttcaatt gaaaattatc
    19861 tatttcaaaa aattacaaga aaaaatattc tagaacgaaa acaaacaatt caacatctaa
    19921 tccaaacagt tgaaa
//
LOCUS       DACEOE010000037        19016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEOE010000037 DACEOE010000000
VERSION     DACEOE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.039"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(287..3436)
                     /locus_tag="IW774_001115"
     CDS             complement(287..3436)
                     /locus_tag="IW774_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1153471.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYNTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3450..4694)
                     /locus_tag="IW774_001116"
     CDS             complement(3450..4694)
                     /locus_tag="IW774_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1153472.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(4687..6279)
                     /locus_tag="IW774_001117"
     CDS             complement(4687..6279)
                     /locus_tag="IW774_001117"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1153473.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(6788..7513)
                     /locus_tag="IW774_001118"
     CDS             complement(6788..7513)
                     /locus_tag="IW774_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153474.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(7667..7993)
                     /locus_tag="IW774_001119"
     CDS             complement(7667..7993)
                     /locus_tag="IW774_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153475.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8030..8395)
                     /locus_tag="IW774_001120"
     CDS             complement(8030..8395)
                     /locus_tag="IW774_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1153476.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(8432..8647)
                     /locus_tag="IW774_001121"
     CDS             complement(8432..8647)
                     /locus_tag="IW774_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1153477.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            8776..9111
                     /locus_tag="IW774_001122"
     CDS             8776..9111
                     /locus_tag="IW774_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1153478.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9187..11184)
                     /locus_tag="IW774_001123"
     CDS             complement(9187..11184)
                     /locus_tag="IW774_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153479.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(11282..11557)
                     /locus_tag="IW774_001124"
     CDS             complement(11282..11557)
                     /locus_tag="IW774_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1153480.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11789..12523
                     /locus_tag="IW774_001125"
     CDS             11789..12523
                     /locus_tag="IW774_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1153481.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12562..13704
                     /locus_tag="IW774_001126"
     CDS             12562..13704
                     /locus_tag="IW774_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1153482.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13799..15190)
                     /gene="sufB"
                     /locus_tag="IW774_001127"
     CDS             complement(13799..15190)
                     /gene="sufB"
                     /locus_tag="IW774_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1153483.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15195..15665)
                     /locus_tag="IW774_001128"
     CDS             complement(15195..15665)
                     /locus_tag="IW774_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1153484.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(15652..16887)
                     /locus_tag="IW774_001129"
     CDS             complement(15652..16887)
                     /locus_tag="IW774_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1153485.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(16884..18170)
                     /gene="sufD"
                     /locus_tag="IW774_001130"
     CDS             complement(16884..18170)
                     /gene="sufD"
                     /locus_tag="IW774_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1153486.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18183..18956)
                     /gene="sufC"
                     /locus_tag="IW774_001131"
     CDS             complement(18183..18956)
                     /gene="sufC"
                     /locus_tag="IW774_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1153487.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
BASE COUNT         5524 a         3867 c         3146 g         6479 t
ORIGIN      
        1 ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa acaaaagaaa
       61 aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg gtcatataac
      121 aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata gctgtcttaa
      181 atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc tacacttcca
      241 ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac ctcaattcat
      301 tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc atttcttgtg
      361 ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa tccttactgt
      421 tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc agattagaag
      481 catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac
      541 tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta tctaaagtcc
      601 ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct gttttattca
      661 atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc gcataagttc
      721 ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta ttttcactat
      781 aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct attaatgaac
      841 ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga ttgttcaagg
      901 ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat tcataagcac
      961 gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc gtatgttcat
     1021 caatggattc catggatgaa acctctttta acttaccata tgcttgcttg aatctttttt
     1081 tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct gaatacagtt
     1141 ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta acaattagtc
     1201 cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc aatcctgcat
     1261 actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa cctgtcaata
     1321 gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat tccgctcttt
     1381 ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct aatgaccagg
     1441 aagtattata atactgatta tattcctcaa taattttttg catttcttct tgttggacac
     1501 ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga aaatcaggat
     1561 cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt aaccagtcag
     1621 gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca atggaactcg
     1681 tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt gtataggcat
     1741 tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct tcaaaaacag
     1801 tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata cgattgatct
     1861 gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag attttattct
     1921 ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa accgagccat
     1981 cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga tcatgggtcg
     2041 tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt gtacctgtaa
     2101 agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt tcagcactta
     2161 acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat ttttggaagc
     2221 gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca tctagctttt
     2281 gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt tcacttaact
     2341 ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg gaagaaatcc
     2401 cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta tctaaatctt
     2461 tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa agcttagtag
     2521 atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat cctgattgat
     2581 gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga tgtaacacca
     2641 tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc aagcgatgag
     2701 catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc tttttattat
     2761 ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag gcatttgcaa
     2821 aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa aagatattgt
     2881 tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga taaaaactat
     2941 cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg atcagtaagg
     3001 tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca atttgatgga
     3061 ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga atgagcgaga
     3121 cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca ttttcacctt
     3181 ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct gttttgatcg
     3241 tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta tttgagatga
     3301 ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga cgatacgtcc
     3361 actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc gcttcctcac
     3421 ggtgactaat tttcatttat cttccctccc taaacaaaca tcttttgtaa gaagcctttt
     3481 ttcgtttcct tcagcaaatc taacttacgt tgatgaagag cgatcgtgtc gtcgagttgt
     3541 ttgaagaaag cgccgatttt ttgttgttcc tctttattcc tagtaaaagg aaacttgata
     3601 ttagcgaaat tctcatagaa taagtattct cgtacgcttc cttcagtgtt gcgcttgacc
     3661 tctttgataa atgttggtga cttgataaac tgaagtatga actcattatc taagtcctcg
     3721 ctcattttca caactacata aagagagcta acaatgaccg tcataccaag attgttaaat
     3781 gcaattgatc ctacgtttac tcttgctgga ttataggcga attcatttgg ttcaacgaat
     3841 ttataatttg ttttttctaa atcatccaac ctactaccat caaactgttc tgtttgagaa
     3901 attaaacctg ctttattact taccgaatag gcagggaagt ctaaatcttt cgtgttcttt
     3961 tttccagtct tcttactaaa ctctttgaac ttacgctgtt cccaatcttc cgtaaatcca
     4021 ggaaaccgaa tttctggcac ttttgctccg tttttaggga acattttttg taagaatcct
     4081 tttttcgttt cctttaataa atctagctta cgttgttgaa gagcgatagt gtcgtccagt
     4141 tgtttgaaga agttcccaat tttcttttgt tcttctaata ttggaaattg tactatccat
     4201 tttttcactt ctgacattgg tacgagattt gtaattgctg aacctggatt agctcctgtg
     4261 agttttcttt gcataaaatc tgcacgaata aattgaataa aatattctga atctgtgatt
     4321 tctttattta ttctaagtct aatcaaagcc tgattgaata cacccggttt tatttgcttt
     4381 ggtactcttg aaattctccc aattgttcca gcgccactca tgataaaatc gcctggttct
     4441 aatttaaatt tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa
     4501 ttatcataaa tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat
     4561 tctttcttta aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag
     4621 cattcaaact tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt
     4681 atatcattac tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg
     4741 tgttgtttgc agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg
     4801 tcgtatcttc ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa
     4861 cgtatctaca tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt
     4921 tgccaaatga gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg
     4981 aatgttttct tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc
     5041 aatgaataat acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat
     5101 cgatgtcccg aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc
     5161 tagtaatttt tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa
     5221 gacgatggcc atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa
     5281 atctgctttg gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa
     5341 ggtcgtatct gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt
     5401 gtaaggttca tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc
     5461 ttcggcatct accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt
     5521 taattcttgc ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa
     5581 catcaatgaa cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg
     5641 atccaacgtc acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc
     5701 ggctttcttc cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat
     5761 cacgtcccca tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat
     5821 cgtcacattt cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc
     5881 aaataaccca ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc
     5941 attcaattgg aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg
     6001 cgaaatcgca taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc
     6061 atctgaaagt aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa
     6121 ggattcatct gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa
     6181 aatcagtccc aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc
     6241 tgccgcacta aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt
     6301 atttaaagtt gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa
     6361 aaaagaaaga aacttattct tgctaataaa ctacgcgaaa ggagtctaac gttgtttgat
     6421 ataacttaga ctgatcgtta acttattttt tatatttcta catagttatc tttagaacca
     6481 tttcagatac atatgaaaca attacatatg attttttcat aaaaaaacag tactgtctag
     6541 tagtataaaa tatacaaatt tcttaaaaat tttttcacta aaatcactac ctgaaacagt
     6601 ctaaaacact tatatatcaa cgtttaatga aactatctcc gtttcatttc agatacaaat
     6661 aaatatagtc gggctccaaa tccgaaaaaa aataccccca gtagcttatc ctaagctact
     6721 ggagatgtat aagtacggtc atataacaac ggggtagggt aaataaatga aaaagatgga
     6781 agaatcatta ttcaataact tttttaaata cttcgttgta atcagttacc tgtccgttct
     6841 taaataccac agaataatct tttttcccat tttcaagtaa ccgaagtgga atatctttac
     6901 caactttctc tgtcactgca ttactgatac ctgttagatt agctaataaa tctgataaag
     6961 attgagcatc tgtagaaatc tctgtatcag tatttatcgt gattccttta tcttcattta
     7021 caagtttcca agtaggtggt atagctgtgt atgtttctac aaatgaatta agtttttcat
     7081 taagtgactt ctcttttata tttccatcta aaaactctgt atatatggct tgagctatac
     7141 taaaacaaag ctggccttgt tctaataatt ttacatcatt agttagtaaa gctgtgttca
     7201 cgttatcaat cgcttcaaga agagtatctt tggtgtccct aacagcttta ggtgtgcctt
     7261 ctggaatttc cctacttgat acctcgtcca ttttatcttc cataaacccc ttaaaatcat
     7321 atctttctcc caaatccata gaagtttttg agaaagcttt catttctgag tctatttctt
     7381 caataatatt taacatgtct gatacatatt gtttaggctg attcgttttt aatctatatg
     7441 tggtaaatat ctcaattcct ccaattatca ataaaattgc caaacaaatc cataataatt
     7501 ttttcttttt catttttcct cctatacact gctgttataa cagtgactaa tgtaaagatt
     7561 ttcccagcaa tcatcagact attatttaca gggaatcaaa aaaactcctt agtaagctta
     7621 cacctttagg agttcctgtt tgaacggtat gcataataca tttaaatcaa tttaacatta
     7681 acacctcagt atatggtatc tctacatata cacggtcttt ttttggatag tactcgacat
     7741 ttatattgat agcacttcca ttgtctaacg tagctttagc ccggtacgtg ttatcaccat
     7801 tatcatgagt gatcgtcaca tcatcacatg tccgatttaa gttatactgt tcttgtaaaa
     7861 tctgagttac caatggtaca gatgacttct ctagtccaga tgaacccgtc attcttgaaa
     7921 aaattatacc tataatagcc aatacaacga tacctataat gacaaacttc ttattcatat
     7981 ttactttatt caacttttta tctcctatct ccaaaatgat tttctctcat cagtaactca
     8041 ttgacatccc caatagtaca ccaaagatgg ctccagctac ccctgctatc atcaaaatcg
     8101 tacccatttg ttcatctttc tctcgatata agtaacccat aataacagca acagcaccaa
     8161 ataccacagg aataaatgct aatgatacta ccatagaaat ccaaccaata atcaaataca
     8221 gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt gtctgctcag
     8281 tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtaat gctccgcaaa
     8341 actggcaata cttactttca tttgttattt ctttaccaca ttttctacaa tacatgtctt
     8401 ctcctcctaa aatcattcaa ttgcttatta gctaactgtt tactttatta aatacatcag
     8461 aaaacagctc atttattggt acacttaata tctcagaaag cattttcatt tgatagggtt
     8521 taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca ttcatttttt
     8581 tggctaactc agtttgggaa agtccttttt cttttctgta tcggctgaga ttacttactt
     8641 ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat ttcaactcat
     8701 aaaatatata aatacttcaa atgtcattac aaatacaaca acttgttgta ctctactgag
     8761 aaggggggtg tattaatgaa acagaagcaa tatgcaaagg tcattagaga aaagcgaaaa
     8821 gcactcgggt ggacacaaaa agaattagct aaaaaaatct tctctacaca acaggcagta
     8881 gcaagatggg aaaattctgt aacagaacct aacctcgata gcttaacagc tctttctaga
     8941 gccctaggaa cccctgtaag ccattttcta gacaatgttg acatagatta tgaggaagag
     9001 tttttggctc tctatcgctc tcttagtact gaagatgcaa ttcgaacaat tgactatatg
     9061 aagctattaa aaaggcaaga aaatgaacgc aatcagctat tgaaagattg aaaaataaat
     9121 aacccaccga actttaacgg tgggtgtatt gagatgaaat aagattaaga gctatggata
     9181 acctgtctag tttaatataa ttgggtctct tctattcctt actctccgtc catcatctct
     9241 tgttacactt gtaaattgat aaataggaac taattcaact cctgataata tcaattcttt
     9301 tattacttca tctatccctg cccctttcgc tagatatact aaaaaactac tgcttgcttt
     9361 cacattgtat agtttcttaa tatccttaga agctcgttca agagtgaatc cttgtttaac
     9421 aaagtagcct ctgttttcta ttaaagcatt tttcacgctg ttatccctga catttcgtag
     9481 gcgttcacaa acagtgttaa taagttcttt ttctgtcatt agatatgtcc cttgccacga
     9541 taagccctgt aaaacttcat gtgcttcatc taaaatcaat tgtttttcct tgctaagttc
     9601 tgatcgggaa ttttgaggaa atactctttt agcctctttt tcgttaaaac atatcttaac
     9661 ccattcataa gaaaaatatt gatatgtaaa atgaaatttt tccagaagtg gaaggatatc
     9721 ttcattcaat ttttgaatca cagaagttgc attaaaaacg tctaatcgat agacattgct
     9781 tctcttatgt ttaaagtttc ctaacgcttt ctcttccttt aacctattta ggtattcgag
     9841 catcttcaaa ggaacatctt catcatctaa tttttttatc aatcctataa aagtcataag
     9901 tgatagtaac ttattacatt tatctatgtc ataagtttta ccatagtaag aactcatttt
     9961 tttagctaat tgttcccccg acaaaataca agtcacactt tccttactat catcaaaaaa
    10021 attaacatca gaaagcaaga catcatagac acgtcgcatt tcctctagat aattccttat
    10081 tgtcctgaaa agtttgtctt tcattgattc aagagtgtca aaaagtactt ttgattcctc
    10141 tttcaatttt cgaaaatgtg taggatacaa gtctaggttc tctaatagcc aattaaatgt
    10201 ttctacccga ctgtctttct ttaaaattag catcacaact ccaataaaat tttcactgat
    10261 atcacaatta ctggaatgac aataaaaaat ggaatgccca gttttactgg taaaaatact
    10321 agcagaggga ttatgatcgt tgtgaaacag acatcttaag tttttacttg acttaagtaa
    10381 tttattcata tcaatggtta acaatctttc ataaatctca ttaaaagaca tgtcggagga
    10441 atcaaacagg cagtctttaa accactgttt catttcttct ttgtcgtcat tttttatcaa
    10501 cttaacaaaa tcggtttcat tgactagttc atactctctc cctgaatcac tttgacaatt
    10561 tttagaacat gtcatggtaa aatcaatcgg aagctcagta atatctaaca tgttgtcttc
    10621 attaattatt atcactgcat ctgacttata tcctccaaaa aacattctat tcgaagcatt
    10681 tacattgtca tcacacccgg ggatgttctt tttcaagtat tcgtaaacag caaccatttt
    10741 accatagtca gtaattgctt cttttaatag aaagacaatc ttgtattttt ctagattttc
    10801 agagtaccga atactttttt gtatcgcaaa cgcagaatta tgaactaaat tgatagataa
    10861 aaccttttct atgcttaagt atctactatc atctttactc aaatctacta ccttcccctt
    10921 ctcatcttta acagtgttat cgacatccag aataattact ctggtttcca caaaactctc
    10981 cttgtcattg cctttattct ttgtcttgct tggaataaat gattgtgatg aatttagtat
    11041 ctctacaaac tcatctcttg taacttctct atagttatct aaacacctat ctcttacatc
    11101 agttttttct ttttttgtaa ctggcttctc taataatttt tcttgatcta cataaattgg
    11161 aatattaatc attgatgttt ccatttgtgt ttcctcctta taaaattctg ttaaagacta
    11221 aaacatacag ataataaaaa caaactcttt attactatat gttttagtct tatttgttta
    11281 ctcaaccttt ataccgttcc atgaaactat caatatcaga ttttttaaat ctaatgaccg
    11341 tatcaatttt tataacaggt aacccgtatt gagaaacata ttttctaatc gttcctactg
    11401 atacacctag atattcactt gcctgctgat agtccatata ctctagagtg actccagaaa
    11461 taatgtcttc tagcataggc tttagcactg taattatctc tttcacttgt tgttcaactt
    11521 gtatagaaac aatgctatca atgatttgta gagacatatt tccccctcct ttcgtgattg
    11581 cttgaacaaa ttttctaaga attatcttcg tattttttgc tcaaaataaa agtattttgt
    11641 tattaaccga ttaatcaaac aaaaaatgat taccagttca tcgacataca cataatacct
    11701 cgataaaaaa ggggtgtcaa cagctttgta aaatttattt acaacacccc ttttttgagg
    11761 tatactaatt ttaggaggtg tggtcaatat gataaaacgt ccaccaataa attatttaga
    11821 acgaaaaaag atactcggaa caaaaataaa agctataaga aagagtaaaa aattaacaca
    11881 accagcattt ggtcttatga ttaacaacgg tcaattaatt gacaaaaaaa ctatctacga
    11941 atgggagaag ggaacctatt taccaatacc agagcgatta tcccgaattg ctgatttggg
    12001 aaatatgagt atagaggagt tagtttgtgg caatgtagaa gaatatattt tgggaattat
    12061 tctttataga gatagcattg tactagatgg tataacattt ccagataaaa acctgttcca
    12121 gcatcttcgt caacagttcc caccagtaca ctctaattta gatacatggc tggatagata
    12181 ttccaaatta gaaccagaga tgcaagaatt tattgctaac aagacttgta ataaagttaa
    12241 aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa gaattattca tcaatgctat
    12301 tgtagaagaa tttgacaata acattctttt cctcactagc agtatagaag aactactaga
    12361 aagaatggta gatgaatggc tacctattca attgaaagac atgtcttatc ccgaagaagc
    12421 tgtgagagaa attactgata atataaacaa gctagaacaa acgatttcat caattgggaa
    12481 aaaatacact aagaaaaaaa tgaagggagg tgataccatc tagtggtaag tactccttgt
    12541 aaactaagag gagatgattt aatggcaaaa atatcaaagt acaaaaagaa aaacggtcaa
    12601 gtggcgtgga tgtataaagg acacattgcc accgatccac gaactggtga aaaaataaat
    12661 actacgagaa gaggttttag tagcaaacaa gaagctcaaa aagattatga cgagtacaga
    12721 catcaaatat tatacggtgt taaaaagaaa gcacctgata tgacatttga agacctgtat
    12781 aatgaatgga taactcatca gcgtaccagt gttaaagctt caacaatcgc aatctcagta
    12841 agatacgcaa ataatcaaat acttccagca tttggtaagc ttaaactatc gaatatctct
    12901 gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta aatatgaaag ttacgattat
    12961 atgagaaaac agactgcaca aattttacgc tacggggttg caatgcaata tattgacaat
    13021 aatcctatgg aaaaaacact tcttccacga aaaaaagagt atgagaaaaa tcgtaaattt
    13081 tactcaaaag aggagcttaa taatttactg gatgctttta aagattttgg taacatgaaa
    13141 caatacgcct tcttcagatt attggcatac acaggtatga gaaagagtga agtactcgct
    13201 ttacagtgga aagacataga cacgtttaat aaagaactac atgtcaataa aactcttgct
    13261 gtagatgaat ttggtaaagt tataattcaa tctccgaaaa caagagcttc tagacgagtt
    13321 atctctttag atactgagac tctttctatc ctcaacaact ggaaattaca gcaaaaagaa
    13381 gagtatttaa aacttggata caatactagc tctaaagagc aacatgtttt tacaacagtt
    13441 aaaaacactc tatacattcc aaacactgtt aatgattggt taagatatat tttgaaaaaa
    13501 tataacttgc ctcgtattac acctcatggg tttagacata cacatgctag tttattatta
    13561 gaagcaggag aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa tagtaaagtg
    13621 acattagaca tctatgcaca tattacaaat aatgcaccta agaaaacagg tcaagacttt
    13681 gctgatatga tggctcatca gtaaaagagc tggtcaaaat ctggtcattt ctagtataca
    13741 ggcaaaacaa aaaagctaga acccttatat atcaagggtt ctagctccta taacaggttt
    13801 acccaacact gccttccatc tcataactga tcaatcgatt gagttcgact gcatattcca
    13861 ttggaagttc tttggtgaat ggttcaacaa agcccatgac gatcatttca gttgcttcag
    13921 attcggtcag gcctcggctc atcagatagt agagttgttc ttctgagatt tttgagactt
    13981 tggcttcgtg ttctaaagag acttggctgt tgtggatctc attgaatggg atcgtatcgg
    14041 atttcgacca ttcgtccatg atgatcgtgt cacattcgat atgagagatc gaacccgcac
    14101 tgtttttggc aaaggttact tgtcctcgat agtttacttc tccgccgtct tttgaaattg
    14161 atttagagac gatagagcta gatgtatttg gtgcattgtg gatcattttt gcacccgtat
    14221 cttggatttg gtttgctcct gcaaaggcga tggagagcat ggtgcctcgt gcaccttttc
    14281 catctaagta aacacttggg tatttcatcg ttgtttttgc acctaggttt ccgtcgatcc
    14341 attcaacggt tgcgccttcg tatgcttttg ctcgtttggt tactaagttg tacacattgt
    14401 ctgaccagtt ttggatcgtt gtgtaacggc aatacgcatc ttttcttgta aagatttcta
    14461 cgatggcggc atgcaggctg ttacttgaat aagtaggtgc cgtacaacct tcgacatagt
    14521 ggatgcttgc tccttcgtca acaatgatca gcgtacgttc gaattgcccc gtgttttccg
    14581 cattgatccg gaagtacgtt tgtaatggaa catcgacttt gacgcctttt ggcacgtaaa
    14641 taaaggtccc acctgaccat acggctgaat tcagcgctgc taatttgttg tcggttggcg
    14701 gtacaagttt tgaaaagtac tctttgaata attcagggta ttctttcaaa gcagagtcag
    14761 tgtcagtaaa gacgattcct aacttttcga attcatcttt catattatgg tacacgactt
    14821 ctgattcata ttgtgcggaa gcaccggcta gataagctcg ttcagcttct ggtataccaa
    14881 tcttttcaaa tgtatctttg attttttctg gtacgtcttc ccagtcacga gcgggtttgt
    14941 cacttggttt ttggtaatat ttgatggcat cgaaatcgat gtctgaaagg tcggggcccc
    15001 atgtttgcat tggcattttg ttaaatgttt ctagagattt cagacggaat tcaagcatcc
    15061 attctggttc gcctttgatt cttgacatct cacggatcac ttcttctgtc aagccttctc
    15121 ctgtactgaa aaccggttcg acatcatcgt gaaacccgaa tttatattct tctaattctg
    15181 gtacgcccat gttctcactc ctcttccttg tggtaatgat ggaaagctgc ggtaccatcg
    15241 ttttccattg cttttcctag tgctttccag gcaagtgtcg cacatttgat ccgagcggga
    15301 aatttagcta caccaccaag catagcagca tcgcctaatt ggtcgggatc tttgacttct
    15361 tttccttgga caagctgtag aaagtctaca gttaaatgtt ctgcttcttc tattgatttt
    15421 ccgatgacgg cgtctgtcat catcgaggca cttgccgtac tgatggagca gccactgccg
    15481 tcaaaggcga tatttttgat gattccgtct tttacatcga cttgcaattc gatcacatct
    15541 ccacatgtgg ggttgttcaa ttcgatcttg cttgtcgagt cagtcaagct tccacgatga
    15601 tgcggatggg aagaatgatc caagatcact tgacggtata aattgtctaa tttagatagt
    15661 cccatgttgg aaaaactcct ttgttgccat gattgcttca actaaacggt cggcgtcttg
    15721 cgttgtgttg tagaaataaa agctcgcgcg agcagtcgct gatacttcaa ggtacttcaa
    15781 tagcggttgc gcacaatgat gacctgctct gacggctacc ccttccatgt ctaaagctgt
    15841 cgcgacatca tggggatgta acccatccaa attgaatgca acgactcctg tccgaaaatt
    15901 cgggtcttgt ggaccataaa cagtcaatcc atcaattgct tgtaattttg gcaacacata
    15961 agcggctagt tcttcttcat atcgatggat attgtccata ccgagttttt gcaaataatc
    16021 gattgcatgg cctaatgcga tagcgccggc gatatttggt gtccctgctt cgaatttcca
    16081 aggtagttct ttccatgtac tgtcgtataa ttcgacaaaa tcgatcattt cgccaccaaa
    16141 ttcaacaggt tccatttctt ctaaccattt tcgtttacca tataaaacac caatacctgt
    16201 tggtccgcac attttgtgtc cgctgaaagc aaagaaatca cagtctagtg cttggacatc
    16261 aatggccatg tgcggggcag attgagcacc atctactacg atgaccccgc cgttttcatg
    16321 gacaagttca gctaattctt gaacaggatt gaagacacct aagacattgg atacatgagc
    16381 aatggagacg attttggttt tgtccgtaat ctttttcgct gcatcctcca tatccaggaa
    16441 gccttcttct gttagttcga tatatttcaa gacagcacct gttcgttttg ccaactgctg
    16501 ccacgggata acattagaat gatgttccat gtaagagagc actatctcgt ctcctgcttt
    16561 gatatagcgg tcgccgaagg ttctggctac ccaattcaag cttgtcgtcg taccacgagt
    16621 aaacagcact tctgctgttt cttttgcatg gatgaacgcc cgtacttttt ctcttgcttg
    16681 ttcataatca tgggtcgcgc gttctgctaa cgtatggaca ccgcgatgaa cattggcgtt
    16741 atcgtgttcg taatagtacg ctaatgcctg gagtacttgt tttggctttt gggtcgtcgc
    16801 tgcattatcc agataaacta aaggttcatc attgacgtct tgaaataaga taggaaaatc
    16861 attgcgtagc tttttagcat cgatcatcca ttcaacttcc cttcgattac ttcgactaat
    16921 tctttccgaa cttgttctac agggattgct gttaagacag aaccgaggaa tcctcgaatc
    16981 actaagcgct ctgcttcttc tttatgcaat cctctactca tcagatagta catttcttct
    17041 ggatcaactc gtcctacact ggcagcatgt ccagcagtta cttcgttttc atcgatcaaa
    17101 agaataggat tcgcatcacc acgtgcttta tcagaaagca tcaagacacg actttcttgt
    17161 tgggcatctg caccttttgc ccctttcaag atgtggccga taccattaaa ggtcaatgtt
    17221 cctttttctc ggatcactcc gtgttgcaaa atatgcccga tcgtatgagg ggctttattg
    17281 gttactcgtg tatcgattcc ttggatttgt ctgcccgagc tgattgccac gattttgact
    17341 tctgcgtgtg caccttctcc tgctaaatct gagtcaaagt cagcgatcac atgaccgtca
    17401 ttcatgacac caattgccca gtcgacagaa gcatctcgta aaatgtgccc acggcgattc
    17461 atataagaag taatattttc tcctaattga tctacggctg aatatttgat ttttgaacca
    17521 gctttagcga tgacttccac gatgatatta cctgatgatt ttgctacttg ttctttggtt
    17581 gtctggaaac gttccagata agaaaattca ctatgttcat ctgccacgat caacacatgc
    17641 ttgaagaagt gctcatcgga tgcgccatct tgaatgaata aggactcgag tggttcatcg
    17701 atcacgacat ttttaggcac atacaggaaa ataccgctgt tcataaaagc tacatggagt
    17761 gcagtcaatt ggtcttcgtt catgtttact gcttttttca tataatattc ttggactagt
    17821 tctggatatt cgatcattgc agtgaacaag tcggtaaaaa tgactccttt ttctgctaat
    17881 tccacaggca gttgttcaaa aatcgtccat gaaccttgtt ggacaagcac aggattgtct
    17941 ttcattgcat caaatgccgg aatcgtgcct gtttcactga tggtttgtgt ttctttcaca
    18001 tcaaataacg accagcgatg gaatttcacc cgatcgatat gcggcaatgg cagttcatct
    18061 gctttcgcta gtgcgtcttg acggaaggtt ctcatccaat caggttcgcc tttacttaaa
    18121 gaaaaatcat ttaccgcgtc aagataatcg agccagtttt tttctttcat tcttgctccc
    18181 ccttacgctt cttcttcttt atagtcgatt cctaattctt tactgatacc agcgtagcct
    18241 tctgcttcca gacgtttagc taggtcagca ttccctgtca tgactactcg gccttccatc
    18301 atgatgtgaa cgacatcagg tgtgatatag ttcaataatc gttgatagtg ggtgatgatc
    18361 aatgcaccga aattgtctcc gcgcatttca tttactcctt tagcaacgac tttcaaagca
    18421 tcgatatcca gacctgaatc gatttcgtct aaaattgcaa atgtaggttc taacatcaac
    18481 aattgcagga tctcattccg tttcttttct ccgccagaaa aaccttcgtt cagataacgt
    18541 tcagccattt cttctggcat atttagtaaa gccatttttt catctaactt tttcaaaaat
    18601 gccataacag aaattttgtc gttttcatca cgtttcgcat tgatcgctgc acgcatgaat
    18661 tccgcattcg tgatacctgg tatctcactt ggatattgca tagctaaaaa taagccaagt
    18721 cgcgcacgtt catctacttc caagtctaaa acatttttcc cgtcaaacaa aatttcgcct
    18781 tctgttactt catagtttgg attgcccatg atagcggcag ataaggtaga ttttcctgtg
    18841 ccgttaggtc ccatgatggc atggatttct ccggttttca ttgttaggtt tacgccttta
    18901 aggatttctt tttcctcgat cgacacatgt agatttttta tttctaagac agacatgcat
    18961 tataccccca aattctcttt cgttctcact cattttagac taattgagaa tgaagc
//
LOCUS       DACEOE010000038        18280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEOE010000038 DACEOE010000000
VERSION     DACEOE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.040"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2470
                     /locus_tag="IW774_001132"
     CDS             <1..2470
                     /locus_tag="IW774_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1153488.1"
                     /translation="SLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLFALDRY
                     SYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQGTYWTR
                     EKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEAANVFY
                     QFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESAFDWSL
                     FQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDRKETKG
                     LLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQSYDTDG
                     SLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLALDEKKP
                     VPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTSISRME
                     NFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLVNMSDA
                     QRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNTGMHPL
                     QTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDYKSSGR
                     SFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTETDDVEK
                     NTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKDKFFTE
                     EELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDVMLKEN
                     NYHRLEKIDKEEALRRILKRSEEE"
     gene            2467..6189
                     /gene="addA"
                     /locus_tag="IW774_001133"
     CDS             2467..6189
                     /gene="addA"
                     /locus_tag="IW774_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1153489.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            6258..6599
                     /locus_tag="IW774_001134"
     CDS             6258..6599
                     /locus_tag="IW774_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1153490.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            6908..7951
                     /gene="pheS"
                     /locus_tag="IW774_001135"
     CDS             6908..7951
                     /gene="pheS"
                     /locus_tag="IW774_001135"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1153491.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            7956..10376
                     /locus_tag="IW774_001136"
     CDS             7956..10376
                     /locus_tag="IW774_001136"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1153492.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(10546..11556)
                     /gene="trpS"
                     /locus_tag="IW774_001137"
     CDS             complement(10546..11556)
                     /gene="trpS"
                     /locus_tag="IW774_001137"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1153493.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            11810..12547
                     /locus_tag="IW774_001138"
     CDS             11810..12547
                     /locus_tag="IW774_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153494.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            12561..13376
                     /locus_tag="IW774_001139"
     CDS             12561..13376
                     /locus_tag="IW774_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1153495.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            13389..14036
                     /locus_tag="IW774_001140"
     CDS             13389..14036
                     /locus_tag="IW774_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1153496.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            14052..14708
                     /locus_tag="IW774_001141"
     CDS             14052..14708
                     /locus_tag="IW774_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1153497.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            14870..15691
                     /gene="racE"
                     /locus_tag="IW774_001142"
     CDS             14870..15691
                     /gene="racE"
                     /locus_tag="IW774_001142"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1153498.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            15694..17049
                     /gene="rph"
                     /locus_tag="IW774_001143"
     CDS             15694..17049
                     /gene="rph"
                     /locus_tag="IW774_001143"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1153499.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            17050..17568
                     /locus_tag="IW774_001144"
     CDS             17050..17568
                     /locus_tag="IW774_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1153500.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            17675..18175
                     /locus_tag="IW774_001145"
     CDS             17675..18175
                     /locus_tag="IW774_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1153501.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6124 a         3134 c         3937 g         5085 t
ORIGIN      
        1 gtctttatat ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat
       61 cgatgaagaa caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc
      121 tcttgaccgc tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt
      181 tctgtcgacg gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag
      241 gaaaaaactg gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg
      301 gactcgtgaa aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga
      361 cgtacagtat ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc
      421 agtatttttg aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta
      481 tcaatttttg atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt
      541 agacagagga aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga
      601 tctgttggat gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca
      661 agaaatcatt acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga
      721 ccaagtgcga atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc
      781 attagggttg aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag
      841 tgaagaacga gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc
      901 aaaggaaagt atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga
      961 tcgattatat cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc
     1021 ttatttgcgt agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac
     1081 gatcgaaagc gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa
     1141 taggatttat cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct
     1201 caaagaatta gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca
     1261 ggaacattta aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa
     1321 agagatttat acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt
     1381 catgcaattt ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac
     1441 tggtgatttt tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt
     1501 atccttagtg aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga
     1561 agtattcgga gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg
     1621 ctaccagtta ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa
     1681 tacaggcatg catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg
     1741 tatcacaggt ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat
     1801 cgatcgaatc gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa
     1861 atctagcgga cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt
     1921 gacgtatctg gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa
     1981 accagcagga tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt
     2041 agagaagaat acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct
     2101 tttagaagta ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa
     2161 aaaaaatgcg aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga
     2221 agaagaactg caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc
     2281 gattacttcc ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg
     2341 cgaattttgt ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta
     2401 ccaccgtctg gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga
     2461 agaagagtga atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg
     2521 caagcgattt ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa
     2581 actacagtgc ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa
     2641 ttattgattg tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag
     2701 gcattacaag aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag
     2761 cagttagttt tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt
     2821 attcgacgtt attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa
     2881 acagaaacga tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca
     2941 gaaaatgatg aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac
     3001 ggcttgacga atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa
     3061 aaatggctag aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga
     3121 ttatatcaaa aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta
     3181 tatgaacaga tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta
     3241 gctggagaac aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa
     3301 gaagcttatg ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca
     3361 gaattgaaag aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt
     3421 cagcagataa gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac
     3481 aaagcactac cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc
     3541 agtatgcgta aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg
     3601 cagattctca cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat
     3661 cgaaaaaaat tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa
     3721 gccattttat actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg
     3781 gatgtcaaac aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa
     3841 tacgagaatt tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc
     3901 tctagaaaag aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca
     3961 gtgggtcaaa tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca
     4021 gagaatgaac agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag
     4081 attgagattc cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca
     4141 ggactaaaaa ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa
     4201 aaaagtcgtc cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac
     4261 ttaacgattt tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa
     4321 aactattttc aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat
     4381 ccctatcaag atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa
     4441 ccagaattag ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt
     4501 gcttatcaag catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa
     4561 ctagagcatt ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc
     4621 tattatgaga cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa
     4681 gccaatcttt atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga
     4741 ttataccagt tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa
     4801 ccagtcattt caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc
     4861 ttggaattcc cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg
     4921 cgcaatcggt atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa
     4981 acacgagtgt tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa
     5041 ttgttgtcgg aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt
     5101 ttcattgtcg gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct
     5161 gatcatgagg agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg
     5221 aattggattg gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa
     5281 atttcgactt ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag
     5341 caatcgatca ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag
     5401 cagatgaaag aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa
     5461 tatccttatc aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga
     5521 ttattcgaag atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac
     5581 aaagcagcat cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg
     5641 caaaaagatc ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa
     5701 ttactgccat tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta
     5761 aggaaacggt tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg
     5821 ttctttcaaa cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa
     5881 caaccttttt ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa
     5941 gatgaactat taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat
     6001 atctatgatt ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca
     6061 atcgtccaaa aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg
     6121 aataagccag tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt
     6181 aacaaataat tataattgag caaacttgac ttatgttttg taaggattat gctatactct
     6241 gggaagaggt gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg
     6301 aaaaaacatt ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg
     6361 aaaacggtcc gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag
     6421 tattagtcga acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct
     6481 gtagcgaaaa gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag
     6541 cgatgcgtca aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat
     6601 tttgtaaaaa aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa
     6661 aagagtagtt tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt
     6721 gtttgaaaaa tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg
     6781 ttaacgaagt aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag
     6841 acctcgttcc ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa
     6901 caagcacatg tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat
     6961 cacagcggca caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa
     7021 aggaccgatc acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa
     7081 agtcggaagt ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa
     7141 acaagtatta gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac
     7201 actacctggt agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga
     7261 aattgaagat atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc
     7321 tgatcattac aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca
     7381 agatactttc tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc
     7441 acggacaatg gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa
     7501 agtcttccgc agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg
     7561 gctagttgtt gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat
     7621 gaaaaaaatg tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac
     7681 ggaaccatca gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt
     7741 atgtaaatac actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt
     7801 gaagatgtca ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga
     7861 ccgcgtagct atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt
     7921 acgtttctta agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca
     7981 atcgttatgt agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga
     8041 caggaatcga agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg
     8101 gtgaagtcaa ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg
     8161 atattggcga agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa
     8221 tcaaagtgat tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag
     8281 gaaaaatgcg tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt
     8341 cagatagtgt tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg
     8401 taaatggcga tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta
     8461 tcacaccgaa tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa
     8521 tttatcgtca gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta
     8581 gcgataaaat tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa
     8641 tcattgaagg agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg
     8701 aaggcattcg tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg
     8761 gacaaccact acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc
     8821 gtgcaaaaga aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg
     8881 acaatctcgt cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt
     8941 tagactcaga gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc
     9001 caatttcgat tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt
     9061 ttgaaaaagg aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga
     9121 tcgtttctct tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag
     9181 cagaagacgt atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc
     9241 ttgtcgttaa agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg
     9301 agtatgttta ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata
     9361 ttatcgaaga agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg
     9421 gagaaacggt tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa
     9481 tctttgaagg aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat
     9541 caagacagtt tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag
     9601 aacgttctgt attacgaatg aatttgatca gcggactatt agatgatatc agttataaca
     9661 ctgcccgaaa aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa
     9721 atgatccacg tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta
     9781 tttgggaaga aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg
     9841 gtcttttaga aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag
     9901 tgacagaaat gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat
     9961 ttattggttt tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa
    10021 catatgttgc tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt
    10081 tcgaagcagt gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa
    10141 ctgtgacaaa tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag
    10201 atatccaatt atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg
    10261 cgtatagcct gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag
    10321 caatggaaaa agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag
    10381 gaaaatatag taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg
    10441 tgacacaact tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag
    10501 ataaaaaaac aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt
    10561 gattcccatt gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc
    10621 actaccttta cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg
    10681 cgcacggatc ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac
    10741 atcaccaagt ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt
    10801 tccgaataca tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac
    10861 atggatgtgg tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg
    10921 atcggagata taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc
    10981 aggtaatcta cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc
    11041 atatgtatga ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc
    11101 ctcaccaaca ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg
    11161 atagataaag aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt
    11221 gacagtcgga ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa
    11281 ttcagctaat tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc
    11341 cacggctaaa tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt
    11401 agcattatct gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc
    11461 tgcctgcata gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc
    11521 agttggtcga tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt
    11581 aagtctgagc acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa
    11641 aaaacaagga aagaggataa aataactaat agattagaaa tggaagaaat atttatggga
    11701 gaataaacgt tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt
    11761 atacatcttt aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat
    11821 taaatttgat cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct
    11881 cgaattcgag aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac
    11941 gatgcttcgc tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga
    12001 tactgacttg catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt
    12061 tttccaacat ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc
    12121 aatcaaagtg ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa
    12181 aactgtcaat atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca
    12241 gcaacgggtg gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga
    12301 acctacttct gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt
    12361 ggctcatgac ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt
    12421 ggcggatcgt gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag
    12481 agactttttc gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa
    12541 tcattgatag gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag
    12601 gcattgcttt tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt
    12661 tagagcgaag caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat
    12721 ttggaatgat ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag
    12781 caatcactaa aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta
    12841 aaactcggat tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga
    12901 tcacagatga acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag
    12961 ctttgttggt aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa
    13021 cggttctagc agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg
    13081 caaaaatttt agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg
    13141 gagacgcgat gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt
    13201 atgagttagt tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc
    13261 aagaaaactt cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat
    13321 acgataaaat ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg
    13381 gagaggcaat gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga
    13441 tcacattgtt ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg
    13501 caatcttcca attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat
    13561 tttttagaaa cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt
    13621 attggtttaa tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg
    13681 cttttattgc tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag
    13741 caggactatc ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag
    13801 cattcaaaat cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat
    13861 ctattttagc aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg
    13921 aaacattcaa taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc
    13981 taccgttatc ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg
    14041 aggggaaata catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg
    14101 aaggattaaa ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg
    14161 gtggtctgac aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg
    14221 tagtgattga tattatacgg aatcttccat tattattgat cattttcttc acttactttg
    14281 ccttacctca aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga
    14341 tttttgagtc agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag
    14401 gacagatgga agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg
    14461 tgttaccgca agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga
    14521 tcaaagatac ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta
    14581 ttatttatgg acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt
    14641 acttcgtggt atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca
    14701 gttattaata gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct
    14761 ctttccattc gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt
    14821 cacgctaaaa agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt
    14881 gacagataat cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa
    14941 agaagccctg aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg
    15001 cccatatggc cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta
    15061 tctggtggag caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc
    15121 tttagaagaa atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac
    15181 tagagcggca gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac
    15241 ggtaaaaagt caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac
    15301 aagtcttgct tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt
    15361 ggcgaaaaaa attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt
    15421 gattttggga tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga
    15481 aaatgttcaa ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga
    15541 ttatttcaat ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac
    15601 tggctctgcc aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa
    15661 tgtagaacat atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa
    15721 gagcttcgca aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg
    15781 atccgttttg gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct
    15841 tttttgagag aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca
    15901 acgagtacga gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa
    15961 atccagcgtc ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa
    16021 cgtagtatca tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt
    16081 atcactgggg catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa
    16141 ttgtcagaag atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat
    16201 gggacttgcg tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat
    16261 cttgtaatga cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg
    16321 tttgatggtg aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg
    16381 atcaaagagc aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa
    16441 gaaacaaaaa caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat
    16501 atgttcaaag aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat
    16561 gtggaagaaa ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca
    16621 caattgcttg atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc
    16681 ggtatgccag gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat
    16741 aacgccaaat tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc
    16801 cattgtacgt tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg
    16861 cccggtcgag tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt
    16921 atcccagaag gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc
    16981 agtcatcggg ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga
    17041 gagagataga tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt
    17101 gacttagtag atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa
    17161 ttagaagctt ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat
    17221 ggaccaggat tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact
    17281 cacggtcatt tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct
    17341 gcaactgccg atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag
    17401 aaaatcctgt ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca
    17461 tcatatgctc ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc
    17521 catcaggcag ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc
    17581 taatttttaa taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag
    17641 tagtgttgaa tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat
    17701 gctgctggaa aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta
    17761 tcatcatcca ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc
    17821 tgtattagga aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa
    17881 aatgattgac ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt
    17941 gacagaaaca gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt
    18001 gttagtcgat tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat
    18061 tacaagaaaa gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa
    18121 tatattgtct ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac
    18181 gagaaactaa aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta
    18241 tcttgaaaaa gaggctggga caaaagcgtt taactccaag
//
LOCUS       DACEOE010000039        18211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEOE010000039 DACEOE010000000
VERSION     DACEOE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.041"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(46..1938)
                     /locus_tag="IW774_001146"
     CDS             complement(46..1938)
                     /locus_tag="IW774_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153502.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1935..3662)
                     /locus_tag="IW774_001147"
     CDS             complement(1935..3662)
                     /locus_tag="IW774_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153503.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3678..4328)
                     /locus_tag="IW774_001148"
     CDS             complement(3678..4328)
                     /locus_tag="IW774_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1153504.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4532..4828
                     /locus_tag="IW774_001149"
     CDS             4532..4828
                     /locus_tag="IW774_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1153505.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5020..5640
                     /locus_tag="IW774_001150"
     CDS             5020..5640
                     /locus_tag="IW774_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1153506.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5732..6898)
                     /locus_tag="IW774_001151"
     CDS             complement(5732..6898)
                     /locus_tag="IW774_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1153507.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6910..7263)
                     /locus_tag="IW774_001152"
     CDS             complement(6910..7263)
                     /locus_tag="IW774_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1153508.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7391..8413
                     /locus_tag="IW774_001153"
     CDS             7391..8413
                     /locus_tag="IW774_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1153509.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8852..9286)
                     /locus_tag="IW774_001154"
     CDS             complement(8852..9286)
                     /locus_tag="IW774_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153510.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9396..11255)
                     /locus_tag="IW774_001155"
     CDS             complement(9396..11255)
                     /locus_tag="IW774_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1153511.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11373..12532)
                     /locus_tag="IW774_001156"
                     /pseudo
     CDS             complement(11373..12532)
                     /locus_tag="IW774_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(12839..14263)
                     /gene="araA"
                     /locus_tag="IW774_001157"
     CDS             complement(12839..14263)
                     /gene="araA"
                     /locus_tag="IW774_001157"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1153512.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14279..14977)
                     /locus_tag="IW774_001158"
     CDS             complement(14279..14977)
                     /locus_tag="IW774_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1153513.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14934..16595)
                     /locus_tag="IW774_001159"
     CDS             complement(14934..16595)
                     /locus_tag="IW774_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1153514.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16813..>18211
                     /locus_tag="IW774_001160"
     CDS             16813..>18211
                     /locus_tag="IW774_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1153515.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
BASE COUNT         5187 a         3850 c         3274 g         5900 t
ORIGIN      
        1 gacttttgtc acagccccta aaaaaaataa taaatttgta ctattctatg aagcttcttc
       61 ttcagaaaac tgactgttat aaagatctgc atagaatccg tctttttcca tcaatgtatc
      121 gtggtttcct gtttctacga tcgaaccatg attcatcaca ataatgttgt ctgcatcgcg
      181 tattgtagac aagcggtgag caacaacgaa acttgtccgg ttttccaata ggcggttcat
      241 cgctttttga atcagtattt ctgttcgtgt atctacacta gatgttgctt catcaaggat
      301 caggacatct ggatttgcta agaatgcccg cgcaattgtg attagctgac gctgcccttg
      361 cgaaatattg cttgcatcct cgtttaatac agtgttatat ccttctggca atttacgaac
      421 aaaatcgtct acgtgagcag ctttagcagc ctcaatgatt tgttcgtctg ttgcatcgtc
      481 gttcccatat ttgatattgt catagatact tcctgtaaac agccaagtat cttgcagtac
      541 cattgaaaac tgtgcgcgca attcatcacg tgaaagatca cgggtatcta caccatcgta
      601 acgaatacta cctccagaaa catcgtagaa ccgttcaaga aggttgatca gtgtcgtctt
      661 ccctgcacca gtcggaccta caatagcgac catctcacca ggtttgacat ccaagttaaa
      721 atcagtcatt agcaagttat ctggtgtgta gccgaattga acattttcaa aacgaacttt
      781 gtatggtgta tctttttctt ctggaaggcc agatttttca ttggacatct cttcttcatc
      841 caacacttca aatacccgtt cggctgaagc aactgttgat tggatagtgt tcatcaaact
      901 tgcaagttga gtgattggtt gagagaactg tgttgtatat tgcaagaacg cttgaacatc
      961 ccctaatgtc atgttcccat ttgcaacttt tactccgcct agtacagcga cgaacacata
     1021 gcctagattt ttaacgaaat tcatcaacgg catgatgatt gccgaaataa attgagcttt
     1081 ccaaccagcg gcatacagat tctgattttc tttttcaaat acttcctggt ctttttcagt
     1141 atgattgaat gttttaacaa tggtatgacc accatatgtt tcttctactt ggttattcaa
     1201 taaacctaaa cttttttgct gatctgcgaa aaacacttgt gatttcggcg ctacgatcat
     1261 cacgatgatc aagcttaaag ggatcatcaa taacgcgact aacgtcaatt gccagctgat
     1321 tgaaagcatc atcacgatga ccccaatcaa tgtgactgca ctagttacaa attgagtcaa
     1381 attttgctgc agcgtgcttg cgatgttatc catatcattg attgctcgag acatgatatc
     1441 cccatttgta tgtgtatcat aataggaaat cggtacacga ttcattttgg cttccaaatc
     1501 atgacgtaat tcataaactg ttcgttggga aacacgtgtc ataataaatt gctgtaaaaa
     1561 gttgaataca gcagaaatca aatacatcac gattactgtc gcaatgatct gcgcgatttt
     1621 atcaaagtct atcggaaatt ttgttacttg ttggccttgt tgcatctgtt gagcgccagc
     1681 cattacacct ttgaatattt ctgtggttgc tttccccaag acctttggtg tttggatttg
     1741 gaagatcacg gctgctattg ctagtacaaa tacagcgatg atcgcaacca tacgtttaga
     1801 catatagcca aacaatcggc gtaccgttcc ccagaaattt ttaggctttt caccttttac
     1861 accgagattt ctgccaggtc caccatgtcc tggaccgcct gctggtccat gagagggacg
     1921 tgtgtttgtt ttactcatgc taagtcctcc tctcttagtt gtgaatgaac gatttcttga
     1981 taagtttcgt ttgttgctaa tagttcttca tgtgttcctt tgccaaccag ttttccttca
     2041 tctagcacaa ggatcatatc tgcatccatt actgtactta ctcgttgagc aactaaaacg
     2101 gtgatacttt ctttcatatt cgttttcaat gcttggcgaa gattggcatc cgttttgaaa
     2161 tccaacgctg agaaggaatc atcaaacaca tagatatctg ctgttttgac taatgcccgt
     2221 gcaattgcca gacgctgtct ttgtccacca gagaagtttc ctcctccttg ttccacatgg
     2281 ctatctaagc catcttccat ctctgaaaca aaatctttag cttgtgcgat ttctaatgct
     2341 ttccaaatca tttcatctgt tgcatccggc ttaccatact gcatattgtc tcggatcgtt
     2401 ccagagaaca gaactgcttt ttgaggaaca aagcccatta attctcgcaa attatattga
     2461 gtcatgtcac taatgtccgt tccatttatt tggatcgaac cagattcgat gtcatataat
     2521 cgagggatca aattgactaa ggtcgtttta cctgaaccag tcccccctat gattgcgacg
     2581 atctcgcccg acttggcttg gaaatcaata tcttccaatg caagtttttc tgctccatga
     2641 tatcgataat tgacatgatt gaaggccaat gttgcgtttt ttccagcaaa agaaactgtt
     2701 ttaggatttt cagggtcatg gataccgatt ttttcatcca acacttcatt aatacggtca
     2761 gctgaagctt gtgcacgagg caccatgata aaaatagcgg aaagcatcat aaagctgatc
     2821 aagatctgca tcgcataagt cataaatgca atcagattac cgacttccaa agtcatatct
     2881 gctatgtagt gtccaccaaa ccaagtgatt gctacgtttg taccgctcat gatcaatgtc
     2941 atcattggca tcatcaatgc gacgatcgta ttaactttga ttgccgtatt tgtataatct
     3001 ttattcgcta aatcaaaacg attttcttca aagcgtgtct tatcgaatgc tcgaatcact
     3061 ctgacccctg tcaatccttc tcgaaatacc aggtttaaac ggtcagtttt cttttgcata
     3121 cttttgaata atggaacagc aaagtacatg attccgccga tcaaaataat cattactggg
     3181 atgacaacta agaagatttt tgtcaactga tgatcttttt gataagcaag tatgctcgcg
     3241 ccaatcaacg tgattggtgc attgatcatc atacgtaaaa acatttgtgt aaccatttgg
     3301 atttgagtca catcattggt cgttcttgtg atcaaagagg ccgttccata tttatcaaac
     3361 gcatcttttg tcagactctc tgatttttta tagacatccg tacgcaattg cttacccagt
     3421 ttctgtgatt cacgagtagc aaaaaatgta tttgcgatag cagctaagat actgatcaaa
     3481 gaaaagccga tcatcacaaa acctgtatgc caaatataat cgacatctcc ttgcgctacc
     3541 cctttatcaa taatattgga ggtaagtgtc ggcaaatata aatctgccag tacttggatc
     3601 accatgaaaa ctgcagctgc aattgctgaa gtcaaggaga tccgttttac gatttttagc
     3661 atctagttcc cttctttcta tttatatact ccgtttttca gccaagaaag tttcaaattg
     3721 aattcactga ttgcttgctc tactgagtaa tcttcagatg ttgaaaataa gcgatacgaa
     3781 tgcgcaattg ttgtaaatac agtattcatc actaattgga tcagtaattc caattcatga
     3841 tcatttttta ctttcagctt cgtccgatcg atcaattcaa gcaactcttt tgcatcttgt
     3901 ttgtgttctt tatgtggatc gtgaggcgtt ttttggcgac tcgtatgagg agcgactttt
     3961 cttgaggcac ggtaatccat gttcataaac aagttacgat aaaaagaagc attctcgcct
     4021 tccaaaatct cataaatcat tttagaaaaa taggattcga atcctttgaa tagatcacca
     4081 tcggattctt tcattagttg taacatatct ttagagctgt cacgtctcat tgtctcaaag
     4141 taataaaaat aaagatcctc tttatcttca aaatactgat agaaactgcc tctagggata
     4201 tccgctaatt tgacgatatt agcaatcgaa gcttctttta agggtactct agaaaactcg
     4261 attctcgcag cctcgattag tcttttttgt ttttctttag gcaaatggaa aaatgtctgt
     4321 gtcggcatat ggattctcct ttctttcaag atgacacatt gtcatatgac gctatgtcat
     4381 tataacacct cctacctgtt ttgcaagaca tttgcatagt ttttttcatt atcatccgat
     4441 acattaaaaa agtgataaga ggaatccttc catttgaatt aatgagagtt gcccattaaa
     4501 atagccacat actaaacaaa ggatgagata aatggcccaa tcaattagtg tccaagactt
     4561 caataaattg cctctcgatg ctgacagcat cgttttagat acaagaaata ccttagatta
     4621 cacaaaaaac cacttaagcc actcgctttc tatccccgct gctatcttgc caagacaatt
     4681 aaaaaatcta gatccaaaga aactttatta tattctaagc tatacaggca ggcgatcaga
     4741 agcattagcc gatcaactta ctgctaatgg ttttcgtgct gtttatatca ttggtgggat
     4801 ggaagctctt cagtcgagtg ttgcttgaat attgcttgtt agattcaaaa gaggctgtga
     4861 caaaaaatcg tgtcacagcc tcttttatac cctgtctttt tttagttatt tattcttcaa
     4921 ccttatcaat atgacttgat agccttttct ttttcgagta aactgatttt gttagtcata
     4981 gttataacaa tcatagcaat aaaaggagaa ggtaagcata tggcatctgt tttagtagtc
     5041 aaaggtcatc ccctaactgc agaagaatcg cgcacagtta aggcattgac gtctttttta
     5101 acaagttata aagaaaatca tccagatgat gaagtgactg ttttggaact ttatcgcgat
     5161 gatattccgg aaatcgatga agaattatta tcaggttggg aagcattacg tgctggtgca
     5221 gaattcactt ccttatcaga aagtcaacaa caaaaaattg ctcgtttcaa tgaattgact
     5281 gatcagttcc ttgctgctga taaaatcgtt atcgcaaacg ccttatggaa tctaaatatc
     5341 ccaacaaaat taaaagcttg gttcgatacc gtcaatgtag ctggaaaaac gttccgctat
     5401 acagaaaacg ggccagaagg attagtgaca ggtaaaaaag cactgcacat ccaatctaat
     5461 ggcggtgtat acaacggaca agactttgcc tctcaatatg taaaaggtat tttaaacttt
     5521 gtaggcatcg atcaagtaga tcagctattc atcgaaggaa tcgattatga ccctgaccgt
     5581 gcagatgagt tgatgcaaaa tgcattagat aaagctgctg ctttaggtaa atcattttaa
     5641 aaattgagat aaatgcatac aaaagagaca gaggaaaaaa tcttcaaagc agcatttgtg
     5701 ccttggatat tttcccatct gtctcttttt gttattcttt ctgctttttc aacggatttt
     5761 cttcataaag agaagagatt gttgtgtacc attcaaaaac atgagtaatc actactttag
     5821 ctgctgcata aacaggaatc cccaagatca caccgatgat tccgaacaat ttcccagaag
     5881 tcagcagaac taataaaatc gttactggat ggattgctaa ctgattccct agtactaatg
     5941 gagagatcaa gcgtccttct attgtctgct ctactgcaaa aacgatcagt actttgatga
     6001 tcatgaatgg acctgctaca atggctaaaa agatagccgg aatcatcgct aaaaatgatc
     6061 ctaagtaagg gatcaggttt aaaaaacccg caataatccc caacgtgaca gcatactcta
     6121 aaccaatgac cgcaaaacca atcataaaca tgattgccac agcaaaagcc acagttagtt
     6181 gtcctcttat ataagaagaa acttgactat tcatttcact cagcacattc aacgtcggtt
     6241 tttgcatgcg tgtcggtaaa aatttgacaa aataaggtgc taattgttta ccgtctttca
     6301 atagataaaa taagataaac ggcatcgtca gcagagccac taaaatagtc gctaccgcgc
     6361 cgacaaaact tccaagactc tgaactgtac tcttagatat atcttggatc atatcgcctg
     6421 ccgaattcat gaacttatca cctgctgttt ccaactgttc gcgaaattgg ttgaacagcg
     6481 ggtcccgcaa aatctcggtt aatttattat caatcgtatc tacatattga gggaagttac
     6541 tgatgaagct cactgtttgt tcctgaatct tagggatgat cactacgctc ccccatgcga
     6601 ttagtgcaac aaccaaaaca aacagcccaa tgatactgta aagccttggt acttttcgct
     6661 tttcaaagta atcaactacc ggattcatca agtaatacaa gattcctgct aaaatgatag
     6721 gcagacctac gattgctaaa aattgccaga ttggtgcaaa caaatatgaa acttttgtaa
     6781 ataaaaagac gatcagcaat attaacaata caacaagtaa ggctgtgact acttgattat
     6841 ttaaaaacca gcgccaaaac cacgataccg gtctgttctt cttatttttt ccttccataa
     6901 ttcctcctgt tagttataaa aaatttggct tcccaaacca atagcaggca gtctatgcgg
     6961 atgaagatag attccatttg caatttgtcc ttcttctgga caagcatcaa aaatcaatga
     7021 tacgtgagct actgtcttta aattcccatt ggccatcgga ccgacataat cgatcgtata
     7081 tacctgatca tcaaaagcaa ttgttccgcc tgctttcaaa gaaaaaggtt cgtcttttga
     7141 tacttcttga atcaccgaat attttcgtaa ggcagctgtt gctgttttat cgaacaaaat
     7201 cagcatgggt tcttccttgt ttattgcttc tgtcccaatt tctgtaatct ttgcctgaat
     7261 cattgcactc tctcctttaa ttgctttcaa aaacagtata acacattctc attctttagc
     7321 cgtgtggaat aattatgtga tagaataaaa acgaaaacta tttcattaca attaggagga
     7381 atcagctcat atgatcgaaa agataatttt aggaacctat acgcgtcgcg aaagtaaagg
     7441 aatctatacg atcgaccttg atacagaaaa agaagaatta gccaaccttt cattagctgt
     7501 tgaagaaaat agccctacct atgtagcaaa aagtaaagct ggcaatcttt acacagttac
     7561 tagtgtggat ggattaggtg gtgccggtgc gtacgataag gattttcatt ttttgaatgc
     7621 ggtaacagag tctggcgctc ctttgtgtta tgtagcggtt gatgaagcac gtcaattact
     7681 atacggggcg aactatcata aaggtgaagt aaatgtctac catatattag gagatggcga
     7741 actgaaagca gcagattcaa tattccatca agaagcgaca ggtcctcata aaaaccaaga
     7801 tcatgcgcat gtccactata cggatctgac tcctgaccaa cgattagtcg tatgtgatct
     7861 tggtacagat cgtgtgtata cttatgatgt gtccgaaaac ggcaaattga atcttgtaac
     7921 taccttcact gcagagccag gaacaggccc tcgccatctt gtctttcatc caaatggaca
     7981 attcgcttat ttatttggtg aattagacag cacagtccgc gtgctttctt ataataaaga
     8041 aacaggtgct tttgaagaat tacagaaaat atcgaccttg ccagaagaat tcgacggtga
     8101 aaatggaggg gcagctatcc gcatttcaaa tgatggcaag ttcctttatg cttctaaccg
     8161 aggacataac tcgattgctg tattcgcagt ttttgaagat ggttcccaca ttcaaaacaa
     8221 gcaaatcatc tcaacagaag gggattttcc ccgcgatttc gcactagatc caagcaataa
     8281 ttttgtcgtg tgtgccaatc agaacaccga taatctgaca ttatatcgcc gtcatcctga
     8341 aacaggatta ctggaattac tgaaaaaaga tgtctatgta ccagaatgtg tctgtgttta
     8401 ttttgatcgt tagtaagaaa aggagaatga aatgaatcag caagacgtat tagacagact
     8461 acgggaagaa ctaaatattc ctttctttga tgggaaaatc gaagataaag agtatagcga
     8521 agaggaatat cagaaactca aagccgattt acagaactac tttgaacaat atgtacagaa
     8581 tgtagaaaac taatcagctt tccatactat ctgaaaagaa catcaggaca agacttttcc
     8641 gtctatcctg atgttctttt ctacaaataa aaaaccgccg agatggttag actcgacgga
     8701 aaaggagtat ttactcgatt ttgagtaagt acaaggttgt tgttggttat aggtatgtca
     8761 ttatcataaa ggattttgat cactctatca taaaggtaat attacattta tattagataa
     8821 ctctagtttt tattgttatc aaagatttct tttatttttt caacaacaaa ttagtcattt
     8881 tttggatggc agctgtcaat tggtctgtcg cttggtcttg ttttgcttca gatgtattga
     8941 atagatcttc atctacccct gattcaagat cgatactttg gatcatttct tcacagcctg
     9001 ctacatatga aagataagct ttttcaaaat ttttatgaat gcccattgct tttgctggtg
     9061 gacgcaaagt actgactttc ataagcattg cccgatattt ttttgttcct tcttcaaata
     9121 acaggatgat ctcttcaaga gtcgatttgt ccagttcact tgtttttcct tcatcaattg
     9181 cgtttctgat cacttcatat ttcggatgca ttccttctcc gattgcttct gtttccttga
     9241 cgatttgatc gatggactgc acataatagc caatattcgg taacatgttc gttttcctac
     9301 tttctccatt gttgttatta tttattctac ttgtttttat cctcaaatga caagtacttg
     9361 aaaaaattta atggtttcta tacagctcgc cattcttatt cgtgctcaaa gaaaccatct
     9421 gctaaactat attgtttccc tgcgacaatg tcatattgga tcaattgata ttcgtcgtaa
     9481 atctcttgtt gcgagcgatt gaattgcgct tctttatacc actgtccgtt ttgatagtac
     9541 agttctcgtt caaacgccgg aagttcctgt tctaatgata aaagcagctg ataaaagcca
     9601 tttgttgtct gattcgtctg ttccattaga ttcgctgcaa aataaaacgg actggtgatt
     9661 gcatcgtgag tcgtttgctt ttctaattct cctttactgt cgaacataag aaattgtgtt
     9721 tcatgcaatg tttgtttgtc attttttgct tggatcgtat ctgaatagat ccctggaaga
     9781 tgatcccccc aaaaaacaac taaagtcctt ctagaaagat tcttcaattc ttccgtgaac
     9841 tgcttcagtg ccgtgctgct ataggaaata tcttgcagat aattctctaa gtcaagtgtc
     9901 ccacttccat ctgaagtttt tgcaggatag tctagattat catactttcc atcgtagggc
     9961 atatgtgtct gcatcgtgac taaatgaatg aactgaggtg ttttttcttc cttgagaagt
    10021 gtcattactt ctttgtaagc tgactcatca gaaatatatg gattattctc tatcgtatcc
    10081 gtataggtca tcgtacgctc gctgatgaac tggtcaaatc ctaatgtctg ataaacatcc
    10141 tctcttttat acatcgaagt attgtatgga tggatcgctg tcgtctggta tgattgtgcg
    10201 tttaacgctg aaacgataga aggaagctga tccatttttg gtacaagcat cgtgtaaggg
    10261 gtggtgagtt gcgcattaaa taacgccata gaaaaaccag tcaatgcttc aaattcgata
    10321 tttgctgttc caccgccata gttttgagaa agcatattgc cactatatgt ttgatcagct
    10381 acttcataat aatctgccaa tggctcacca gatacttcta cgccgtttaa tcgactagga
    10441 tctgagaaac tttcactcat cacgaacacg atgttcggcg actcttcctc tacagtttta
    10501 tttttttcat cggctagctt ctggtatttt tcagtgattt ctttgatctt ttctttcgaa
    10561 tatccttcag gttcatccat tggttctacc ttcagattgt acaaaaaccc accgataaaa
    10621 ccagtgttat agtagttcat tttctggctg taaggtatcc atagtgccgt tttattatat
    10681 gcttttcgca acaaattatc gggattgttg aaattactga tataccctag cagtccaatt
    10741 gccacaagca gtgtcgttaa tcggatcaat tggaatcgcc ttcctttttt caggcttcga
    10801 aacatgtacc agcaaaacaa tcctatcacg gttcctgcgg cagctaagat cactgtaaac
    10861 agcatagtgc cggtcatatc tttcagcaat ccgatttcag tgatcatctt caaatcgtct
    10921 ggataaatcg gttcttgccg ataactcatt ttcatataat ttgcaaaacc taatacaccg
    10981 atggctacga catacgtaag ggaacctaaa gggacagatc ctgctagtgc aatcagaaaa
    11041 ataagaataa tcagcaagac gagacaggct aagaagaatt tttccgtatg ccaagaaaaa
    11101 gcaaatttca acgcaaggtc tacggataga tcattttgac accactgcaa aaataaatta
    11161 ctaaaaacaa caagaaaaaa gatactgatg attgctgctg ttccttttaa aatctttttc
    11221 tgtttggtcg aaagtctgct tatatatctt ttcaaatgct tgctccttca tttctatcaa
    11281 taaaagaatg ttgcttctct tattttagtt gtacgaaatt cgaaagtaaa gaacggaaag
    11341 aagaattaat gaaatccaaa tattctcttt ctttatggcc tttctggttt ctttaataag
    11401 cggacctttc gtactccaga aattttttct agctgttgtt tcacttgatt gagctgttca
    11461 aaatcagtac tgtctatgtc aatcaatatg tagacatgct cattcttatg gttacgcgat
    11521 atatctgcga tcgctagctc gttctcattc agcagagaaa atatctctgc taatatattg
    11581 gttttctttt gataaaatat agtgaatcga aacggcgctt ggaaaaacat tcgtgcggat
    11641 ggataattga ccgcttctct aaccgtaccg aacaaaagat aatcttttaa ggcttcaact
    11701 gccaaacgtc cgctgtctgc caaagcttcc tgagtcgatc ccctagatga ggcaacataa
    11761 gtatacggtc attttctaag aactcttctt ctggaaaatc agtcaagtat ttagccagat
    11821 gttgtttttc caatgcttgc ataacagcat atttatcaac aatttcactt cttcctacat
    11881 tgataagtac agcatctttt ttcatttttt ctatattcgc ctgatcgatc aatccttttg
    11941 tatcttctgt caacggcagc atgactacga tgtaatccga ggtagaaaga actgtttcta
    12001 aatctgcttg ccgaacataa tccaactggg catcgcggat agaataaccc aagacatcca
    12061 tccctaagct atagcaactt agcgctactt ttgtgcctat cgctccaaga ccaagaagac
    12121 cgatggtttt gccttgcaat tctcgtccca cataagcact tcttttgttt tctgcctgtt
    12181 ctaaaatatt aggacctgtt agtgtttgta ccatacgact tgcttcgatt atcggacggg
    12241 aagataagag caaacagcac aaaacaagct cttttactgc attagcattt acacctggtg
    12301 tattcatgac gatcgtcccg ttttcagaag ctttttcaac gttgatcgtg ttcaccccaa
    12361 caccagctct tgaaattgct agcaaatctg gtgtaagcca ttcatctttc actttcgtac
    12421 tacgaataac tattccttcg gctggcaaat catctattat tttgaaatat tccccttgta
    12481 acttttctat ctcttgttct ggaatagcat caatcaaatg aacgtgatac ataaaacccc
    12541 ctccttacta acaagtatct cgaaatttct gcaattagac aactgtttac catttgactt
    12601 cctagaagca aaaagagcag ttagagcaga gtaggctgag tgataaaaat aaggctatga
    12661 caagttactg ccataagcct tattttcgag agcaaaatat tgagggtgag atatcttgat
    12721 aaatggtgtt ccgttagctg acattgataa taagactaaa tttcaaaaaa gttgaaaagc
    12781 aaatttcgtc aatctcttct tatttcttaa aactaagtgg ctttttcaca acctcttttt
    12841 atctaataac cactgtttcc aactcaacta attttgccca cgtttcaatt tgatctgttg
    12901 ttagatttag cgagacaacg gtatgatgac cgccacctgc ctgaatccat gaatggacac
    12961 cttcatggaa attcggttta gttttccata acacacgtgc aacaggtaaa ttaggtgctt
    13021 cagtcgttgg ttcaaaggct tctacttcat tgatcagcag tttataatgc gtaccgaaat
    13081 ctgccatcga tacgactacc ccttctcctg ctttcccatc aaatacaaga cgcgccggat
    13141 cttctctatc tcccattgat aaaggagaaa caacgattct tggtttcgtc gcagcaagcg
    13201 tcggatctac ttccatcata tgggattcca agattgcttc ttggccacta gctagctcgt
    13261 aagtataatc ttccatgaat cctgtatctt tccctcgagc catgattttc agcaaccgat
    13321 caatcgctgc agttttccag tcgccttccc cagcaaaacc gtatccttct gccatcaaac
    13381 gttgtacagc tagtccaggt agttgtttca ttccatacag gtcttcaaaa ttagtcgtaa
    13441 aagcgttata gcctccttct tctaagaaac ggcgaatacc gatttcctgc tgagcttgta
    13501 cttttacatg ttcctcccat gtcttttctt cataatcacc atagtcgaag gtatacaatt
    13561 ccttgtactc ttcaaataat tgatcgactt cttcgtctga tacgcgatcg attacttgta
    13621 caagatctcc gataccaaaa taatcaacag tccaaccaaa ttgaatctgt gcttccactt
    13681 tgtctccttc tgtgacaccc acgtttctca tgttgtcacc aaaacgagcc acttttattc
    13741 caaaactttc attataggct actgcaacat ccatccaatc agcaatctct ttttggatct
    13801 caggtcgttt ccagtagcca acgacgattt tattttgttt atttaatcga gcattgatga
    13861 acccatattc gcggtcacca tgagcggatt gatttaaatt catgaaatcc atatcgatcg
    13921 ttttccaagg aatgctttcg ttatattgcg tagctaaatg aagcaatggt ttttgtagaa
    13981 gctttgtccc gcggatccac atttttgctg gtgagaaagt atgcatccaa gtgatcacac
    14041 ctgctacttc ttctcgataa ttgacctctt tcatgatttt tgtgatcgta tcagcagtaa
    14101 ctgctaattc ttgaaggcgg attggataag gcaattggcc attttcattc agttcatcga
    14161 ccatttcttg tgcatgtttc tttacttctc gcagtgcttc ttccccatat aaatgctgtg
    14221 atcctacaac aaaccaaaat tctttttctc caatattcaa cataattagc cactcctttt
    14281 atttgttttg tccgtaatac gcattttttc cgtgttttcg taaataatgt ttatctaaaa
    14341 tccgttgagg cagctcttct gaaaatgaat tgagctgtct ggtaaataaa ttcatcttgc
    14401 atacttcatc tagtacgact gcgttcatta ccgcactttg cgcatctttc ccccaagtaa
    14461 atggaccgtg tccgtgtaaa agtacccctg gaattgctaa gggatcaatt ttgcgttctt
    14521 tgaatgtttc tatgataact tttcctgttt catattcgta cccgctgtcg atttcctgtt
    14581 gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt atctgcatgc gtcgtcccca
    14641 tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc ccatgtcgag tgagtatgga
    14701 cgattcctcc gatttgagga aatgaacgat ataaaacagc atgagtaggc gtatccgaag
    14761 agggattgag tttaccttca atgatatgat tgtccaagtc cacaacgacc atgtcgtctg
    14821 ctgtcatctc ttcataactg actccgctag gtttgataac gaaatatcgt gtttcaggat
    14881 caaaagcgct cacatttccc catgtatatt tgacgagtcc gtgtttaggc aagtcaagat
    14941 ttgcttggta tacttcttct ttcagttgtt ccagcatttt ctatcactcc tttttcttgc
    15001 aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac ggtaacgtct cataaatatc
    15061 tgatagcctg ctatttcttc tgctgaaggt gtgatggtga ttccttggtc ttgtccaaac
    15121 acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc gatccatcat ataggctgct
    15181 aataacgcga ttccccacgc tccaccttct ccggctgtgt ccatcactgt aactggtgcc
    15241 tccatagcag atgctagaat ccgctgtcca acttctggtg ttttaaaaat ccccccatgc
    15301 cccactaaac gatcaatttg gacattctca gatttcaata tatccattcc gattctcatt
    15361 gcaccaaaag cactggctaa atggatccgc atgaaattcg ctaaatcaaa acggctacct
    15421 ggtgtacgta caaataatgg ccttccttca tttactcctg taatgttttc accggaaaaa
    15481 taaccataag aaagtaatcc cccgcaatcc gtatcgcctt ctagcgcttt gttgaataat
    15541 accgtgaata atttatctct gcttatctcc atacccaaag aattggtaaa ttcttcaaaa
    15601 atactgaccc aagcattgat atccgaagag cagttattcg tatgaaccat gcctactagt
    15661 tctcctgaag gtgtagtgac catgtcgatt tccggatgga cattttctag ttctttttct
    15721 aatacgatca tagcgaaggc ggaagtacca gccgaaatgt tccctgtccc tttggctaca
    15781 ctgttcgtag cgaccatacc ggttcctgca tccccttctg gcggacaaac cgggattcct
    15841 ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg attcgcttaa cttgccagct
    15901 tcttctcctg ctactagaac tttaggcaaa atgtgatcca agtcaatcgc tacgtgcgct
    15961 ttttcgatta actgaccaaa aatatttttc atttcttgat ggtaggtttt cgttgaagga
    16021 tcgataggaa acatgcctga tgcatctcct acgcctagta ctttctcacc agtaagctgc
    16081 caatggacat aacctgcaag cgttgtcaaa aaattaattt ctttcacatg ttcttcttta
    16141 ttcaaaatag attgatacaa atgagcgata ctccatctct gcggaatatt gtattgaaat
    16201 ttttccgtta atattcgttc tgcttcttca gtgattgtat ttcgccaagt acgaaatggt
    16261 actagtaact gttccttgct atcaaatgct agataaccgt gcatcattgc actgaaccca
    16321 atcgacccga ttgtcgtaag attctcgcca tagatttcga atacttctgc tgccattttt
    16381 cggtaactga cgcgcagtcc tttccaaata tcttctaatg aataggtcca gataccattt
    16441 tctagtttgt tttcccactc ataactacca gcggcaatag gagtaaaaga tgaatcaatc
    16501 aatacagctt tgatcctagt tgatccaaac tcaatcccta aagatgtacg accatctatg
    16561 acatctaaca tactttgcgc atttacttcc ttcataatga tttaactccc tccttctcgt
    16621 caaacctgat caaaccgtct ttttagtagc gcttacaatg attattgtat acttttgtac
    16681 gtacatgtca agcagttatt tacaagaata caataaaaaa cacttgtttt gtacgtacat
    16741 aagttataaa atgaaattgc ttacaaaatc tgatcaaccg ccttctcgaa attctagaag
    16801 gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat cgtttattta tttttttggc
    16861 gcatttggcg gcattctatt tggatatgat attggtgtta tgaccggcgc cttacctttt
    16921 ttacagcatg attggggttt ggcaggtaaa gcttctttga taggatggat cacgtcttcg
    16981 gtcatgttag gtgcgatcct tggcggctct ttatctggtc ttctttctga taagcttgga
    17041 cgacgaaaaa tgattcttct ttctgcactt atttttatgg ctggttctgt cctttctgca
    17101 tctgcccctc acaatggttc ttattttctg attgcagcaa ggattcttct aggtttagct
    17161 gttggcgctg catctgccct agttcctgct tatatgtctg aaatggctcc tgctagacta
    17221 aggggaagat tgtcagggat caatcaagtg atgattgctt ctggcatgct cctttcctat
    17281 gttgcagatt atttgctaaa agggttgcct gaaacgatgg cttggcgcgt gatgttaggg
    17341 ctcgctgctg tccccgcgct gattttattt ttcggtgttt tagctctacc agagtcaccg
    17401 cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa gagtattgaa ctatattcgg
    17461 acaccaaaag aagctgaaca ggaattcgaa caaatccagc tgaatgtcaa acaagaaaag
    17521 acaaccggaa cttcttggca tactttgttt ttagaaaaat atcgttcctt agtcattgcc
    17581 ggtattggtg tagcagtatt ccagcaattt caaggagcta atgctatttt ttactatatt
    17641 ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg atgcattgat gtggccgatc
    17701 atccaaggaa tcattttagt tgctggttct ttgctctttt tggttattgc agacaagttc
    17761 aatcggcgaa ctttattgaa aataggcggt agcgtgatgg gactttcttt catcctacca
    17821 gctgttttag gaactgtact ggatgctcat acaaattcgt tgttgattct cttgttttta
    17881 tgtatttatg tcgcatttta ttcttgcaca tgggctccac tgacatgggt aatcgtaggc
    17941 gaaatattcc cactagctgt ccgcgggcga gcatctggcc tagcctcttc ttttaattgg
    18001 attggttctt tcctagttgg cttgcttttt cctgtgatga ctgcttctat gtctcaagaa
    18061 atcgttttcg gcatttttgg gatcatctgc tttttagggg ttttatttat tcaagagatc
    18121 gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac aaagtgcatc taaaaaaact
    18181 tatcctaaac gtatttctat ccatcattct t
//
LOCUS       DACEOE010000040        17788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEOE010000040 DACEOE010000000
VERSION     DACEOE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.042"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..191)
                     /locus_tag="IW774_001161"
     CDS             complement(<1..191)
                     /locus_tag="IW774_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1153516.1"
                     /translation="MKPNPVETGKRIKSIRSNFGMTMEQFADLIGNAQKGNINNWERG
                     FSLPNKERLIKIAELSNKT"
     gene            497..685
                     /locus_tag="IW774_001162"
     CDS             497..685
                     /locus_tag="IW774_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ1153517.1"
                     /translation="MPLTGKKMLKLAKKNGWKVVRITGSHHIVAKEGFPPVPIPVHSN
                     QDLKKGTEQKLLKDLGLK"
     gene            731..1108
                     /locus_tag="IW774_001163"
     CDS             731..1108
                     /locus_tag="IW774_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAQ1153518.1"
                     /translation="MKKSYPVIFSAEENGYSVVFPDFAGATQGDNFEEAMSNAKEFLD
                     GILAYYIDEDISVPSPTDIHQLNNLGNNKIVSLIQSDPSPFIQKKTVRKNVTVPEWLV
                     ARGEKDKINFSKVLTDALLKIYE"
     gene            complement(1668..2840)
                     /locus_tag="IW774_001164"
     CDS             complement(1668..2840)
                     /locus_tag="IW774_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153519.1"
                     /translation="MTKKIEVYVDGSQEERDSFFGFFWKNSREYVKALNMVYKQLMFE
                     ELIENSIKELDHEYIKRELSLEERIDNLLSNEVIDQKKLDKTKRELSKLRIAKNKEAR
                     DVLTQAIGLKSQTRIRDIAKKLDFDYSDFIDSAKMKASKDFSNDFVGIVTGQRTPRIY
                     RNKNKYGLLPFRGRDIKIHKADKFYISLPKGFRFKVNLGRKPRKARDSALTLERIMDG
                     QYKLNQSNLIFEGKNLFLNLTISFEKDYHKLDFKEGAELTIKFTDLCSGKCLVNNQSV
                     IDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIIQTAIRNKTQIICISEPDKNYGEWAYYQLKEQIINKGKRYGIEVKSE"
     gene            complement(3084..4052)
                     /locus_tag="IW774_001165"
     CDS             complement(3084..4052)
                     /locus_tag="IW774_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1153520.1"
                     /translation="MSLENEKPYYVVVGGVNGAGKSTIYQVQNLKRKELDTDRINADE
                     ILRSFGGNWKKQADINKSMREVLREIKGHFKEGKSFHHETTFGGNPYPDKMRLLKAKK
                     LGYESFLIYIGLDSPERAIERVKIRVAKGGHGVPEDLIRKRYEFSLRNLAELEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYAWVPKAIQEKNAETPISKQIMMDISQEEM
                     NPKETLERIYSASDDFISDRFSKEAQVDDFYRNHKNEIKERILSISFSTGVSQDELVG
                     QDKRRNSCKLAYINSVGELLVKTDSELLSMELNRDRNIQKTDFEVE"
     gene            complement(4049..4246)
                     /locus_tag="IW774_001166"
     CDS             complement(4049..4246)
                     /locus_tag="IW774_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153521.1"
                     /translation="MRAMTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLT
                     LSSFVTRVDQKKVINQKDFKI"
     gene            complement(4257..4574)
                     /locus_tag="IW774_001167"
     CDS             complement(4257..4574)
                     /locus_tag="IW774_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153522.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRFIEVNNLGIIEDYLDKLDEAIKQSIEKNGEDHILTVVLKDLKDRLEAEAEYF
                     NKD"
     gene            complement(4611..4829)
                     /locus_tag="IW774_001168"
     CDS             complement(4611..4829)
                     /locus_tag="IW774_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153523.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKAIGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            complement(4938..5129)
                     /locus_tag="IW774_001169"
     CDS             complement(4938..5129)
                     /locus_tag="IW774_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153524.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            complement(5291..5647)
                     /locus_tag="IW774_001170"
     CDS             complement(5291..5647)
                     /locus_tag="IW774_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153525.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            complement(5672..6676)
                     /locus_tag="IW774_001171"
     CDS             complement(5672..6676)
                     /locus_tag="IW774_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ1153526.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPVSVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            complement(6713..6973)
                     /locus_tag="IW774_001172"
     CDS             complement(6713..6973)
                     /locus_tag="IW774_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153527.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            complement(6982..8055)
                     /locus_tag="IW774_001173"
     CDS             complement(6982..8055)
                     /locus_tag="IW774_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153528.1"
                     /translation="MSNEQNYETGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            complement(8063..9889)
                     /locus_tag="IW774_001174"
     CDS             complement(8063..9889)
                     /locus_tag="IW774_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1153529.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATNEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSTPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKHDRLLLDRSPENCDY
                     VEKFVVESLKACENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            complement(10044..10910)
                     /locus_tag="IW774_001175"
     CDS             complement(10044..10910)
                     /locus_tag="IW774_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153530.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLVVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWKMVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYESTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            complement(11085..11348)
                     /locus_tag="IW774_001176"
     CDS             complement(11085..11348)
                     /locus_tag="IW774_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153531.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSTVKEFLYSNPKTALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            complement(11350..11679)
                     /locus_tag="IW774_001177"
     CDS             complement(11350..11679)
                     /locus_tag="IW774_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153532.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            complement(11701..13236)
                     /locus_tag="IW774_001178"
     CDS             complement(11701..13236)
                     /locus_tag="IW774_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153533.1"
                     /translation="MVGDDMTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDY
                     ISRQEAIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTR
                     KKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFL
                     VREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAM
                     ERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLEL
                     KQSVKDSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNY
                     MTKDNPKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLL
                     KQSEEKNLKSNELKNLLDGKKLNVKFDREKPEFKTKYAKQSYDDFQKRLEERQLAREK
                     NEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAREFEI
                     GMN"
     gene            complement(13303..13770)
                     /locus_tag="IW774_001179"
     CDS             complement(13303..13770)
                     /locus_tag="IW774_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153534.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKSNMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSTHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEQESSRLGEGTMFSSPSLRFTTD"
     gene            complement(13767..14063)
                     /locus_tag="IW774_001180"
     CDS             complement(13767..14063)
                     /locus_tag="IW774_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153535.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVNKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISERSDLNK"
     gene            complement(14414..14644)
                     /locus_tag="IW774_001181"
     CDS             complement(14414..14644)
                     /locus_tag="IW774_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153536.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
     gene            complement(14680..15576)
                     /locus_tag="IW774_001182"
     CDS             complement(14680..15576)
                     /locus_tag="IW774_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153537.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMIIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKSDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYQNLPKDFGVISVTVNPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKD
                     EKKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQFEEKIKQLEKSASRFE"
     gene            complement(15590..16240)
                     /locus_tag="IW774_001183"
     CDS             complement(15590..16240)
                     /locus_tag="IW774_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1153538.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATPSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDSK
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            complement(16255..17391)
                     /locus_tag="IW774_001184"
     CDS             complement(16255..17391)
                     /locus_tag="IW774_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153539.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            complement(17413..>17788)
                     /locus_tag="IW774_001185"
     CDS             complement(17413..>17788)
                     /locus_tag="IW774_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAQ1153540.1"
                     /translation="INSNNKYACEYVLSFEREMRKMRAGVYFATQTPEEIIPKDSGSE
                     VADVIRNIFNLCTFKCFFNLDSALLEDIKKVLGNTITDTEIMLLPELEVGQAVVQTSS
                     EDTYMINFDPYPEQIERFDGGQ"
BASE COUNT         5150 a         3389 c         2117 g         7132 t
ORIGIN      
        1 atagttttgt tacttaattc agcaatttta attaatcttt ctttgttggg aagggagaat
       61 cctcgttccc aattattaat atttcctttt tgagcatttc caattaaatc tgcaaattgt
      121 tccatagtca ttccaaaatt agaacgaatt gatttaattc ttttacctgt ttcaacagga
      181 tttggtttca tacaatcacc tcttttacag aatttggttt catagtatct tattcgctct
      241 ttgagttcta agttctcttt ctccaatttt tctatataat attcttgatc ctgtttcttg
      301 ttcaaaaaat gacctccaaa tttcacattg tgttacaccc tagtgttaca cctccaaacc
      361 ttattatact gtactttacc atatgataca atagggtata ccctattgtg acacttacat
      421 tctactttta atgtaaaaac tcttgacctt tatacgcata atgcgtataa taatatttgt
      481 aaggaggtag tcgcgaatgc cacttaccgg taaaaaaatg ctcaaactag ccaagaaaaa
      541 tggttggaaa gtggtgagga taactggcag ccatcatatt gtagccaaag aaggattccc
      601 acccgttcct attcctgttc atagtaatca ggatttaaaa aaagggacag agcagaagtt
      661 actaaaggac ttagggctga aatagcccag tcctttagtt cattggatta actcgaaagg
      721 agtggtggaa atgaaaaaaa gttaccctgt aattttttct gctgaagaaa atggctactc
      781 agtagttttt cctgattttg ctggagcaac acaaggagat aattttgaag aagctatgtc
      841 caatgctaaa gaatttcttg atgggattct agcttactat attgatgaag atatttcagt
      901 tccttctcca acagatattc atcaactaaa taatttgggc aataacaaaa ttgtttctct
      961 tattcaaagt gatccttctc catttatcca aaaaaagacc gtacgcaaaa acgtcacagt
     1021 cccagaatgg ttagtagcta gaggggaaaa agataaaata aatttttcta aagttttgac
     1081 agatgcgtta ctaaaaatat acgagtaaac gtaaatatat taataacaat ttagttgcct
     1141 aatgacatag atagcggaat taaaagtaaa atagtactca tttattgcag gttcccttag
     1201 aatcaataca ataaatgagt actatttaga ctgtttaact actagtaaga tatagcgatc
     1261 atcagtttca gggttatccc ttggtgacca tagttttgcg tatttttcaa ctcgttgcca
     1321 aatcaacgat tctatttttt tatttctact taactccaaa acggagtacc ttctttttct
     1381 tcgttagctc ctatttaaga gccatgtcgt tttatataac tctaaaacag aaactactga
     1441 tcaattcaat aaagccttat ttgagaactg tgttgtttta catggtttta aaacctgatt
     1501 tcccgcaaac ggtttatccg taacatttga gaactgtgtt gttttacatg gttctaaaac
     1561 ctcaacaata ttcgtccttc cgtctggtta cacaacttga cagagctaac ccttcagtga
     1621 gtgagtgtcc cctcactatt tcgttgccaa atcaacgatt tcttattcta ctcactttta
     1681 acttctattc catatctttt ccctttattt attatttgtt ctttcaactg ataataagcc
     1741 cattccccat aatttttatc tggttcagaa atacagatta tttgcgtctt attgcggata
     1801 gctgtttgaa taatttttgc cgaacattga ttattatagg ttttaacaaa attgctttct
     1861 cttttcttta tggcttccca tctatcttga tctaattttc tttttcgacc atgaccgcct
     1921 ttagaaaaaa ctcctcggct ttgctccctt gatttcattg ctttaatttt atttttgaag
     1981 ttacttatat atttgctgtc accaaaatca atcacagatt gattgttaac taaacattta
     2041 ccgctacaca aatcagtaaa ctttatggtt aattcagctc cttctttgaa atctagctta
     2101 tgataatctt tttcaaaaga aattgttaaa ttcaaaaata aatttttccc ctcaaagatt
     2161 agattactct ggtttaactt gtattgaccg tccataatgc gttctaaagt taaagcgcta
     2221 tctctggctt ttcttggttt tctacctaaa tttactttaa acctaaatcc tttaggtaaa
     2281 gagatataaa acttgtcagc tttgtgaatc ttaatatccc gacctctgaa cggtaacaat
     2341 ccatatttat ttttatttct atagattcta ggtgttcttt gcccagtaac aattccaaca
     2401 aaatcattgc taaaatcctt actagctttc attttagctg aatctataaa atccgaataa
     2461 tcaaagtcca atttttttgc tatatcacgt attctggttt gactttttaa acctatagct
     2521 tgtgtcaaaa cgtctctggc ttctttgttt ttcgcaattc gtagtttaga taattccctt
     2581 ttcgttttat ctaatttctt ttgatcgatc acttcattac ttagtaaatt atcaattcgc
     2641 tcttcaagac ttagctctct tttaatatac tcatgatcca attcttttat agaattttcg
     2701 atcaattctt caaacatcag ctgtttatat accatattca gcgcttttac atattctcta
     2761 ctatttttcc aaaaaaaacc aaaaaacgaa tctctttcct cttgtgatcc atcaacatat
     2821 acttctattt ttttagtcat agttattatt gtcaatttta gcacctcatt attatatatc
     2881 tggtatataa taatagtaac atatttccta gactataatc aatgatcaat tgtctacttg
     2941 tagtaattat attaaaaaca aaaactaaaa ataatatatt attccttctg actacatata
     3001 gtcactttat ccaaaaaata agaagtgctt tcgcactcct tattaaattt atttactatt
     3061 aattgattca gctacaactt cttttattca acttcaaaat cagttttttg gatatttcta
     3121 tctctattta attccatact taatagctca ctatcagttt ttactaaaag ctcacctaca
     3181 ctattaatat atgccaattt acaagaattt cttcttttat cttgacctac taattcatct
     3241 tgagataccc ctgtagaaaa actaattgat agaatccgtt ccttgatttc atttttatgg
     3301 ttcctataaa aatcatcgac ttgcgcttct tttgaaaacc gatcactaat aaaatcatca
     3361 cttgctgaat atattctttc aagggtttct tttggattca tctcttcttg agatatatcc
     3421 atcataattt gtttgcttat aggagtttca gcatttttct cttgaatagc tttgggaacc
     3481 caagcatatt cttgcgtatt attggctaca actgtatgat tttctttaac ataaatataa
     3541 gaaaactcat ctgaattatc atagatactt acattatcaa aatatttctc taactctgct
     3601 aagtttctta aagaaaattc gtatcttttt cttattaaat cttcaggtac accgtggcca
     3661 cctttagcaa cacggatttt aaccctttca atcgctcttt ctggtgaatc tagtcctata
     3721 tagattaaaa acgattcata acctaatttt ttagccttta gaagtctcat tttatcggga
     3781 tatggattac caccaaaagt cgtttcatgg tgaaaagatt ttccttcttt aaaatgacct
     3841 ttgatctctc ttagaacttc acgcatactt ttgtttatgt ctgcttgttt tttccaattt
     3901 ccgccaaaac ttctcaaaat ttcatctgca ttaattctat cagtatcaag ttcttttctt
     3961 tttaaatttt gaacttgata aatagtagac ttaccggcgc cgttaacgcc cccaacaact
     4021 acataatacg gtttttcatt ttctaaactc atattttaaa gtctttctga ttaattactt
     4081 ttttttgatc tactcgtgtc acaaaactag aaagtgtaag gtagaaagtc ctttcttcct
     4141 cactttctgc attttttaag gcttcatgaa aatcatctac agtcatattc aaaacatcag
     4201 gatacatttt ctttaattct tttacagcct ctgtcatagc tctcattccc ttcttattag
     4261 tctttattaa agtactctgc ttcagcttct agtctatctt tcaagtcttt taaaacaacc
     4321 gtaagtatat gatcttcacc atttttttca attgattgct ttatcgcttc atctaactta
     4381 tctaaatagt cttctataat tcctaaattg tttacttcga taaatcggaa gtccggtaaa
     4441 tttttcatcc gaataccgcc tttaggctgt tcctttacaa ataaagattt gtaatcatta
     4501 tattcattcc cattaataac caattctttc ataggctcta agtggataaa tttacgtgaa
     4561 gcatagtctt tcataataat ctctcccagt agaaaatctt cttttttcta ttatatcacg
     4621 ttctcaaagc gttgagttat tggattgttt aaaccatcaa gacacatcat gctgcatttt
     4681 ttcatcacta ccggtgcttt tccgattgct ttaactaccg ctacattagc taacaaacta
     4741 attacttctc cctcgataag ctctccgctc ccaataacaa cgccctgtac ttccatacct
     4801 actttaaacg tttctttttt atttaccatt attccgctct ccttccatga ctacttgtag
     4861 tcatttggat attttttagg ggaacggttt tccgttccct ttcatcaaat agaatccttc
     4921 atcatttcaa caaataatta actttcagtg taaaaaaatt cttcgtaatt accatcatga
     4981 aaaatatccc atctttgttc gtcattctca ctgaagtagg taaaataata tctgacagta
     5041 tctaacttat ttaattgctc tctggttttg taaatacaat ccaagtgatc ttgcattcga
     5101 tctgaccaaa acattcttgt ctgctccatc tattatctct cctttttatt aaatcaaggg
     5161 tttgagtctt tgactacttg tagtctatgg ctatatcata caggaatgac tacttgtagt
     5221 caatggtttt ctttagttta attaactgtc tattcgtagc caaataatgt aaaaaaacgc
     5281 ctagtttaca ctagacgttt aaacaattca ataattcttc gttatacccc attaagggtt
     5341 gaactgcttt aatacttact tgatcgtctt ctctaacggc ttctatgctt accccttttg
     5401 tatattggcc atctttctta tccgtccaaa tttgataata agtaagccca tatcgttcaa
     5461 taatctcaaa gagaatagtt attaattttt tttgtttaaa ttttggtaat tcaatacgca
     5521 atgatttttc ttgaatatcg aactctggat ttttattttt aagttgttct tcaataatca
     5581 tatagtctgt agacgtctta tctaaatcaa ttaatgaatc tgataagatt gctattgtat
     5641 cgttcataac acatcaacta ctttcttctt tttattccat ttcatataca ggagtattaa
     5701 gagataattt atgtgtgaag ttcacgattt ttgatacatc ttctaacttg aagcgtttaa
     5761 aagcttcttt tcctcttgtt aaattatcat tccaatcaaa tccctctcgc aaaggtacat
     5821 cgacttttac ttcactgatt cctatccctt ctcttccaat tcctttttct ttaaattcag
     5881 tttcaataaa agtacgtccg gctttatcat tatcaacaca aaggactgtt tctgaaggca
     5941 acgatttaaa cttattataa tgctctgtaa ccccatgcaa tacattacct acctttaatc
     6001 cattcatgct aacaaacgta gcattttcgg ctttttctgg cttttgtgta atgtacgaca
     6061 tcaaatctat ttcactttca aaaaaataca ttttatctgg ttgaccaaaa gttacgttaa
     6121 atcctgaata ctctttagag ttttcttgta tttttttcca tgtaacatcc ttttgtttgc
     6181 cttcatgaaa aaattttcta gttccttgtt ccgtacaccc aacgatttct tgatcttttt
     6241 tccataagaa caaaacattt ccttttttat cttgtttaat tagtccttta gataaaaaat
     6301 cttccactaa agctgtatcg attcttctct catttactaa ataagacttt gcaacagata
     6361 ccggcacttc attttctttc tgatattggt acggagcttt tttcactttt gtattgaacg
     6421 aatgaacttc ataatcacta tcttcaagtt tctgttttgc ttctttaaat gaacaatcgt
     6481 agtaggcttt gataaaatca aaaatatctc cccctaaatc ctttgagttc catttaaaaa
     6541 ggtttttacg atcactgatt acacaagaat catgatcttc taatctatag tattcccttg
     6601 aatcttgctt taaacctatc ccattttgtt tgcaaaaatc aacgatactt actttttttg
     6661 cctttttcaa atccaaacaa aaaccccctt tactagacca tcactagtaa aattacgata
     6721 aggtacactt gtatttttca taataatcct ctgtacagaa aataaagttt agcccatccc
     6781 taagagataa ctcccaaaag cctatcttct tgctcttttt ttctctaatt atcttttttc
     6841 taatactttt tccgtacttc aaagccattt taaaagcgat ttgtacatct tcacaaatta
     6901 caaaatgact attattaatt tgacgatatt caccttgcgt ttctaaaaaa tttctatttt
     6961 cttgttctat cattcattta tctactccat ttcaaatttt gttgtatttt caaaattagt
     7021 caatttcaaa atcttattct ccatttcttc agaaacaggt ggtatttcaa acgtatcatt
     7081 ttcaatttca ttataaattt ctctcattgc accgttgtaa gccatgatag acgtagcttt
     7141 tttgtattcc gtagaagaag tatctactgg aaaacctagc agttcttctt tatcgatctc
     7201 taacttttcg gataaatttg tcaatttcgt ttcaatttct tctttatgac tatcataaaa
     7261 ttcattaata tcactatcta atgctaatct ggaaactggt tcttccatgt tttctaagta
     7321 ttcaatgcgc tcttgccaag cttgtttgtc ataataatta gttgtttcta gctcttgctc
     7381 aaaatctttt atcacaactt ctcgaatggg aacttccgaa accttatgcc caacaaattt
     7441 atctgtcatg tcctcactac tttctaagct attccgttcc tcgtttaaat cgtgaatagt
     7501 ttcaatcttt actttatcag gctgtaacac tacgataact acatcctttt gtcttaactg
     7561 ttcttccgca ataatatgtg tatatttccc aacgtcataa aatgccttag cagtagcttc
     7621 atgatctcca attgtatttc caatgtcgtt aatcaaatct acgtcatttt cattctcact
     7681 aagagaagca atcacttctc tttttacaac tttttctccg taataactaa catcattgta
     7741 atcatttaaa atatcattac tttcttgtaa tttatctatt actttagaaa gctcttcatc
     7801 agtaatagtg cgttcattca tagaaatcgg cttctctaat tcaacattta cttcatatac
     7861 gaaaccatta tttccgttac taccctgttc agcgtacatt gtagccattt ctttattagg
     7921 tgttaagtac acaccaaatc cattagctgt tccgtttaaa cctttatgat ctaaactaaa
     7981 ttcatcaaat ttattcatac ttccatgaaa caggcgataa gttccgcctg tttcataatt
     8041 ttgttcatta ctcataattt tttcactcca tttcaaaaga acgttctgct gtttttacag
     8101 tcggcttttc caagttagaa ttttctggta tcttgaaagt tccttcatcc atttcatatt
     8161 ttactttagt tgcaaccagt ttataagcta ccactgaagc cacctcttta tatgactcat
     8221 tgtctattgt atctcttaaa tttgttcctg acacagattc aaaatctgta atcgtatttt
     8281 cgatttcttg tttatgagat tcgtaaaaat cttccgcagt catacaagag tttaattctt
     8341 ttacatcatt aatatttgtt tggttttcaa tttcttcttg ccagcatttt tcattatagt
     8401 aattgttagc attttcagat agattataaa ccgcttcatt tataacaact gatcttagcg
     8461 gttcgcttaa ttttaacgta tcatttttta gttcatgatt ctcgcaagct ttcaaacttt
     8521 ctacaacaaa cttttcaaca taatcacaat tttcaggtga tcgatctaat agtaaacgat
     8581 catgtttatt ttcacttgga ttctcaaaaa ctacaccata aacatcacat acatcaaatt
     8641 gtttcgtatc tgaataatat gtcacatagt tttctcctat tttagctgta acatcataat
     8701 tgctatgagt aagctcaata gaattttctc catactttcc tctataataa tccaccatcg
     8761 cttctaaata actaccgtgg attgttctat ccggatcgtt gattgtacta tcgtagaaat
     8821 tatgaaattc taaatctgat ttttgttgta tctgctcttt attctcatta ggaacaaagg
     8881 aattttttag tgcattaaat gctgtttgct gttcttccaa gactttctta tcaatcgtac
     8941 ttataaagtc aatagacgtt tctctaactt cttgcattat ctgtctaaag ttttttggat
     9001 caattttttc attattttta gtccaatttg caatataagg aatagtaaag tctgacgtat
     9061 caataccgta attgtgacaa acaataaatg aagtcatttc cgcttgaaac tctttaatcg
     9121 gtgttgagta ggaagaaccg tgttcattct tattgtgaag ctttttgtga gccaattcgt
     9181 gaatactggt agtaagattt tgtgttctgc tatttcttga atttaataca atttcttccc
     9241 taccatcaac atattgaata tattcacctc tagcgcttcc catttcacct ctaccagact
     9301 cgttcatatc ttttatagat atattcatag attcggccaa atgatttaca cctttttcta
     9361 attgactaac acttaattca ccatcaatat taaaattcca taccctgtta ggaaatattt
     9421 ttggcaaatc agcttcagtt gcattagttt gggaaatatc aaatacgtaa cccttcttaa
     9481 aatgcttgat ctttttgctt cttaattctc catttttgat tttttctttt tgctcgtttg
     9541 tcgcttcttt taacgtcttt tcctcaccat tttcatcaat aaaaaccgtt accggagcat
     9601 gcgcataaat gcctatccct ttttctttcg ctttaacttg aaatcctagt tttttccaac
     9661 catcataact tgctacagca attgctcctt caaatccctc atcgatcaac gataggttta
     9721 aaggactgta attgtgtatt ttagacatga acttggcaaa ctcgatcata tcttcagggt
     9781 tagactgata ttgctcgatt ttgctgttgg aagccttaat taaatcgtct agctcttttt
     9841 gtttttgctc tttcgttttc ggcttccagt ttcctttttt ataggccatc ttctatctcc
     9901 tttctttttt aggtaagtta aattacctaa ttaaattata ccagatatat aaaaactctt
     9961 ctatctaatt tcactgactt aatgcaaata aaaaagagat aagatagtta tagtaaccat
    10021 cttatccctt cttattttac catctactca actatactta cgtttttacc tttactatct
    10081 aattgaactt tgttatcttt agaagctact ttagtaagca aaagttgatc caattcatca
    10141 ttagaatatc ctgaataaaa attatctaaa actttcgttg tggcaactgg aattgataac
    10201 ttcaaatcca tactagtaga ttcataagat aattctcctt cagacgaaac ttcaccaaaa
    10261 ctatttaaat tccaaattgg actatctaaa tatttataag ataatttaac ggtttccatt
    10321 gaaggtgatt tgttttcatt atccatttta attgttgctg ataccatttt ccactccggc
    10381 tctgaatatt gaacaccatc gatctcttct atatccgcct gtattttagg tatactatct
    10441 tgaaaatcag caattttttg gtcattttga aactcttgtt tttcctcatt ttctaattct
    10501 gtttttacag aatctaaagt ggcattatag ctatcaatat cgcttttttt cttatcatta
    10561 aacttttgtt tatcagcgtt caatgcttgt tctattattt cttcatcact cattttagaa
    10621 actttttcca atgtaagctg ttccatatcc gttttaaata cttttgcttt gccatcttta
    10681 gtttggatca ccgcttttac atattcattt ttatcaatag gcgttttatt tttactactt
    10741 gtttcgacta ccaaccaaca acgtgagtct ttttgttctg cactatctat gaatgagata
    10801 ttatgtgctt catttgactt agcaccagct tcttctttgc cacatgcaga taataatagc
    10861 gcaaaactaa aaatcatagg tattgttgca ataactctta actttttcat ttataagcac
    10921 ctctttttta tcggtctgat atataatttt atattatcat atttttattc caatacacag
    10981 atactcttat ataatgtctt ttcatgtagt ttatcaaatt tctatttcaa accgcttaat
    11041 acaaatatca aagcccaaaa ttttctactg gaagctttta gagcctaaaa tatatttttt
    11101 atttgatcca aaacttcagg gaaatacaaa cctaatatca acacagcacc tataattaat
    11161 agcggaatca ttttcttaat aaaagagatc acagcaaaaa ctatcagtac tgcaactaat
    11221 actaaagctg ttttgggatt agaatataaa aactctttga ctgtagacgt tacatttaag
    11281 tatagttcaa tcaatacgcg aaaatctcca ttacttagtt tatccataac cgttctaaaa
    11341 tcgatcatat catttcctca ctctttccaa ttttattgct tcttttattg aagaagcaac
    11401 ttgttcatta ctatgcttat ctatatataa tggggaaaag taatttttat caaaaaagta
    11461 atttgcaaaa gctacatcgt cttctgtctt ccaattataa aaacagtaat tcccactaat
    11521 cggtatttca aaaagccccc aattctctag ctccatgttt tctttttcaa acaacactat
    11581 gatttttttt acatcttcta tagtttccga atcactgtta agtagctcac gtaaatatat
    11641 attttttgta ttactgcaca ctatatcgaa gtttttcata atttctattc tccgtttcta
    11701 ctaattcatt cctatttcaa attcacgtgc ttgtctcatt ttctcttgct ctatcattcg
    11761 ttgcgctact tcataatcat ttttcgcacg atacatattt aaatcatcat tgattactct
    11821 atcaattttt ctaataagct ttttatcttt aaatttttca gaaatcttct tgaagtggac
    11881 ttcatttttt tcacgtgcta attgtcgttc ttctaatctt ttttggaaat catcataact
    11941 ttgtttagca tatttcgttt taaattctgg tttttctcta tcaaacttaa cgtttaattt
    12001 ttttccgtct aatagatttt taagttcatt acttttcaaa tttttttctt cagattgctt
    12061 taaaagtttg atttgtccta ctacagcatt acccattcga gcattaaaat ctttttttct
    12121 atttaaataa tattcttgtg gattgtagcg tttaccgtag gttgtttgat atagctcatc
    12181 aatttcttta gtcatacgat catattcttt tctttttgga ttatcctttg tcatataatt
    12241 tgtgacttca tcaatctttt cttttgtttc ttcaggaagc tcattgtagc cacgtgttgt
    12301 actaggtatt tcttttttta attcctttag gtactttact aattctttgt catatcttga
    12361 attattttct acatataatt gaatcaaaga atcttttaca gactgtttaa gttctaaagg
    12421 aacttctttt ctcaacttag aaattttttc aaactcgttc gttctatcta atattttgct
    12481 accgaattta aaaatcatat cgtctaacgt agactgtttt ctcttacccc tagcttgtaa
    12541 aacaccatca tattccatta tttcacgagt atttttacgc tccatagctg ttacatgaat
    12601 atggatatgt tcagtatttc tgtgaatcgt tgcaaaccaa aaaggatcaa ctagttcttc
    12661 cttttcttgc atttttccca tcatagactt tgttgcttca tatagaacat tttcatttaa
    12721 ttcatttgtt tctggattat aatatccttc ccttactaaa aaatcattat cgaatgaaat
    12781 tacatcttgg aacatcaccg atccattatt ttttgcttct atgaccgatt cttttatttt
    12841 tgaaatgtct tttttagtga tatatctttt tgtattgcta aatgctccgt ttactatttc
    12901 attatctaca ttttctaaga tagctttttt acgtgtcata taatcaatat atttatccat
    12961 ttcacgtaaa tctttaatca ctggttgatc tgtttcctgt tctatttttt ccaaagcttt
    13021 ttctattcta gctaattcat ttagttcatc ttctgaataa tcatgatcta gtttatcttg
    13081 cctaatagcc tcttgtctgg aaatataatc tacaaatttt tgaagttcta ccatgtgatt
    13141 attctttttg ctttttccag tttcaaattt tgctttaaga ataatgctcg cactttttct
    13201 gttaaagata tgttcagtca tatcatcacc caccattttc aaaaattttt tctacaggcg
    13261 gttttgtact taatgctaag ttgcattaga attttttagt gttcaatccg ttgtaaatct
    13321 taatgacggt gaactaaaca ttgttccctc tcccaaacga ctactttctt gttctggttc
    13381 agcacgctct tcttctaatt ctttactagc taatccacta ttaattaata gtttatagac
    13441 agactcttct atactattta attgttcact ataatcttta tgtgtactgt cttctttttt
    13501 attaaagttt tcaagttgct gtttttgcaa ttctattatc gtttcaagtt cgctaatttt
    13561 taattttgct tcttctaagt ttttagctaa ctcttctttt tctggatcac ggaaattttg
    13621 attggctttt gttaacattg gtaaatttga aacaacataa cggaacatat tggattttgt
    13681 ttgaaatact gacttgttta cttcaaagca cttattgaat ttttctttta catcttctgt
    13741 tgctgatact ttgaaataag ggtagttcat ttatttaaat cactcctttc gctaattaaa
    13801 aaattgaata agcgttcata aactgaagtt aactcgctaa tactttctac catttgttca
    13861 tttgacaata aaagtttttc aactactaaa tcaaattctg actttttata gtcaatcaaa
    13921 tcgatctcgc tatcttggcg aatgattgct aaaaaatagt cattcctact aaactttttt
    13981 ccggtacgtt cttgaatttt attaactttt ttatcaatct cttttacata ttgtggatca
    14041 attcctctga ttgaaacatc catacacaca cctctttcaa gctgttttta tctatctggt
    14101 atatattatt atatcaaagt ccaaaggaaa attaaagatt taaagacaag accaaggaat
    14161 taatacaaag agcataaggg aaaactgata gcaccttcct aaaggaaggt ggctaagttg
    14221 gctgtgccaa ctggttttct ttcaaaatag cttcatattt tttgctatca caaaaaaatc
    14281 cattttcgac ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt
    14341 agttttggtc atagtaacta aagcttttac gcccaaaaaa agagtggcaa gttttacctt
    14401 gccactctct tttctattca caaaattggt ttaaattcgc tttttttagc acttcgttta
    14461 tatcatagca atcataatat ttacacaaca tcattcctaa ttttaacgct tgttcctctt
    14521 tatatttttt aagctctgca ttttttttgc gcttaatcgc ttttctcttt tctttatatt
    14581 ccgcttcaat tctcattaat tcttcttcag ctttctcttg aatagtgaca atagaattac
    14641 acattctctc taaactccct taaaataatt taaacacgat cactcaaatc ggcttgcaga
    14701 cttttctagt tgctttattt tttcctcaaa ttgtttgatt ttattttcac tttcctctat
    14761 tgaagattta ttattttcaa tagtcgtctg catagaactg atttcatttt ttgtatctgt
    14821 ttgttcgtct agggtttgaa attctaagtt ttgattagct ttatcaatat ctttttgcac
    14881 tactttattt gctaaattca ttttttcaat tgattttttc tcatctttta tagaattttc
    14941 tatagcttta atctgatatt taaaattatc tttttgcaaa tcagaagtac tttcaacttc
    15001 caatttagca tttgatttaa tatctttgtc tactgcatag aattttattt ccttttcttt
    15061 taagtcattt gaaggttcta attcaggata aatatatcta gggttaactg tcacagaaat
    15121 tacaccaaaa tctttaggca gattttgata ataaattacc gtgtattcat ctgacaattt
    15181 tacaactttt gtcttaacat cttcattatt tttgttcgtt ataaaattta atttatagtc
    15241 acttttaata ttacttagtg atttagtacc tgtatcatct tgataccgca atactaattt
    15301 aaacagtccg gcttccctat ttatttccat atcatcaaca tacatattca aagaagacgt
    15361 tacattttgg gctgaactca tttttacagt ttcaaaatca taacttttcc ccatcacata
    15421 aggagaaaaa acgattatca ttcctattgc taaaaatcca agtatagcac ctaaatataa
    15481 ttttactcct ttcgtttttt tatatacttt gtcaatttgt gccttatttt ttttatttgg
    15541 ttccttctta ttatgaatgg aatatttctt attcatatac ttcacctcac tattttaagt
    15601 acttgatttc ataattatct accaacactt ggccgtcttt attggttact tcaaaaagca
    15661 tatagactgt attttctgta tctccactag aagattctgt tttataattt acttcagcaa
    15721 aataattaat agtattggcg ttaacataat tcgtatataa ttttgaatct ttatatttac
    15781 ttttgaatgt tggatcactt gaatatgctt ctcctttttg agtagtagat ttactttcta
    15841 aagtcttttt actcgttgta atcatgtcgt ccggcacgat agaatcagct acagaaggag
    15901 tagctatttt ctctaaatca gctttctttt cttctacttt ttcaatatca tataccggat
    15961 atagctcaac aaatttctta gcaacttcag caatttgttt atttaattca ctgccatcat
    16021 cattagttaa gttcgcttct acatttgcat ttagatcact aatattttta cttaacttgc
    16081 tattttgttc ttctagttgt gtaacttctt gatccaattt tttatttttt gcttgaatgt
    16141 tacttgtatg aagttgagat aacaatccac ctaaaactaa aataataatt aaaacaccac
    16201 aaatatattt ccatttatta tcattttttc tcttaatcat ctattttctt cctcctattt
    16261 tttgactcta cgaatctctg gactatgtga ttgccaatat gaatcttgcc aattgtgata
    16321 accaattccg cttgaattac tgtcatacat agttgtttca ttgacataaa tacctacgtg
    16381 agaaatgaaa tcaggcgctc catatgttcc tctaaagaaa atcagatcac cagcttttgc
    16441 ttctttaatt gatactgctt cagttgcttc atactgcgtt actgtataac tatctattgt
    16501 aattccggcg ctaaattgat aaagataagc tgttaatccg ctacaatcaa aacctgtcgt
    16561 tggattttta ccgccccaaa cataaggcca accttgatat ttttccattt ctgttttcat
    16621 ttttccccaa aattctgaag gattagccgg attaccacta cccccaccga tatactgttt
    16681 aactaactct gcatagtaaa aattaccacc atttaaatac aaatatgttt ttccatattt
    16741 aattgatact tcatttacat atgaataagt ctgtcctgta gtatttccta aacttggagc
    16801 aacaacattt ttagagtagg cttcagccgt ttcgatagta tgtgtttttt tgttttttcc
    16861 taagtagtgc atgtaagcta ttccgtaatt gtaagcttga actattccat catagttact
    16921 ttccatgcca taacttttag caatgttata cacattactc aaatgtttta gcccttgatc
    16981 gatactttct tcatattgaa gtgtgtttac aggcaatccg gctgattcgc tactttgcat
    17041 taaatccggc aatttacctc cggattcaca ttgcataatt gctaaagcat aaggtactaa
    17101 ttctttaatt ccgtattttt cagcatattt ttctaccgtt ttttgccatt tttgaacttc
    17161 aggtgacaac ggtttgttgc caccaatgac ttctgcacct cctgtattag ttgcagtttg
    17221 tgttccttgt aatgtagcta aagaacctat aactaaaagg ccaaccaatg ttatcagtcc
    17281 tataactacc cctattttta tttttaaagc aagacctatt ttctttgata cttgcttcat
    17341 cccttgacga gcgagtgttg aagcaacatt attatttttg ttctgctcca ttaaattcac
    17401 ctccgatttc tattattgac caccatcgaa tcgttcaatt tgctctggat atggatcaaa
    17461 attaatcata taggtgtctt cactggaagt ttgaactaca gcttgtccta cttctaattc
    17521 tggtaaaagc ataatttctg tatcagtaat tgtatttcct aaaacttttt ttatgtcttc
    17581 caacaatgca gaatctaaat taaagaagca tttaaaagta catagattaa aaatgtttct
    17641 aatcacatct gctacttctg atccagaatc tttagggata atttcttcag gcgtttgagt
    17701 tgcaaaataa acacctgcac gcatttttcg catttctcgc tcaaaactta gtacatattc
    17761 acaagcatac ttgttatttg aattgata
//
LOCUS       DACEOE010000041        17546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEOE010000041 DACEOE010000000
VERSION     DACEOE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.043"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..970
                     /locus_tag="IW774_001186"
     CDS             83..970
                     /locus_tag="IW774_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1153541.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IW774_001187"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IW774_001187"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1153542.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IW774_001188"
     CDS             2272..2718
                     /locus_tag="IW774_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1153543.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IW774_001189"
     CDS             2991..4343
                     /locus_tag="IW774_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1153544.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IW774_001190"
     CDS             4514..4801
                     /locus_tag="IW774_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153545.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IW774_001191"
     CDS             5027..5470
                     /locus_tag="IW774_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1153546.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IW774_001192"
     CDS             complement(5476..6660)
                     /locus_tag="IW774_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1153547.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IW774_001193"
     CDS             6928..7626
                     /locus_tag="IW774_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1153548.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IW774_001194"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IW774_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1153549.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IW774_001195"
     CDS             complement(8436..8969)
                     /locus_tag="IW774_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153550.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IW774_001196"
     CDS             complement(9266..11647)
                     /locus_tag="IW774_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1153551.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW774_001197"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW774_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1153552.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IW774_001198"
     CDS             12348..12887
                     /locus_tag="IW774_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1153553.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IW774_001199"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IW774_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1153554.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IW774_001200"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW774_001200"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IW774_001201"
     CDS             13994..15160
                     /locus_tag="IW774_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1153555.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IW774_001202"
     CDS             15206..16702
                     /locus_tag="IW774_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1153556.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IW774_001203"
     CDS             16783..17466
                     /locus_tag="IW774_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1153557.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         5931 a         3019 c         3631 g         4965 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttac
//
LOCUS       DACEOE010000042        17221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEOE010000042 DACEOE010000000
VERSION     DACEOE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.044"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..354
                     /locus_tag="IW774_001204"
     CDS             <1..354
                     /locus_tag="IW774_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1153558.1"
                     /translation="YTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSL
                     GEVMDLVSNDGVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVY
                     TSQGKERYVVQGELQ"
     gene            407..2383
                     /locus_tag="IW774_001205"
     CDS             407..2383
                     /locus_tag="IW774_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1153559.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVIYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTEQSKQTPLKGAKFELYKGEPGKGSKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQVGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            2396..3148
                     /locus_tag="IW774_001206"
     CDS             2396..3148
                     /locus_tag="IW774_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1153560.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVSKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            3164..3919
                     /locus_tag="IW774_001207"
     CDS             3164..3919
                     /locus_tag="IW774_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153561.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLVSNGETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSEKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            3932..4192
                     /locus_tag="IW774_001208"
     CDS             3932..4192
                     /locus_tag="IW774_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153562.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            4189..6279
                     /locus_tag="IW774_001209"
     CDS             4189..6279
                     /locus_tag="IW774_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1153563.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRQDAVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            6306..6770
                     /locus_tag="IW774_001210"
     CDS             6306..6770
                     /locus_tag="IW774_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153564.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            6767..7009
                     /locus_tag="IW774_001211"
     CDS             6767..7009
                     /locus_tag="IW774_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153565.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            7027..7329
                     /locus_tag="IW774_001212"
     CDS             7027..7329
                     /locus_tag="IW774_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153566.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            7399..9462
                     /locus_tag="IW774_001213"
     CDS             7399..9462
                     /locus_tag="IW774_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153567.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            9462..12071
                     /locus_tag="IW774_001214"
     CDS             9462..12071
                     /locus_tag="IW774_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1153568.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            12068..14440
                     /locus_tag="IW774_001215"
     CDS             12068..14440
                     /locus_tag="IW774_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153569.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEIKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMKDSRLNQ
                     FKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLSESGETENASVQNIVTREAEP
                     KPIQNVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLRDIKQDIKP"
     gene            14490..14822
                     /locus_tag="IW774_001216"
     CDS             14490..14822
                     /locus_tag="IW774_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153570.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            14823..15416
                     /locus_tag="IW774_001217"
     CDS             14823..15416
                     /locus_tag="IW774_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153571.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            15579..>17221
                     /locus_tag="IW774_001218"
     CDS             15579..>17221
                     /locus_tag="IW774_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1153572.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLLKEKQMEEQDFKRFLAMIDEC"
BASE COUNT         6299 a         2758 c         3526 g         4638 t
ORIGIN      
        1 tacactttga gtcttggagc ggctacttat ttttatgaag atgctgaaat gggcaaagga
       61 aactatgtcc tagcaggtca taacatggaa atgccaggtg ttttattttc tgatattcaa
      121 aagttgtcat taggggaggt gatggactta gttagtaacg atggcgtcta tcgttacaag
      181 gtaacgcgta aatttatcgt acctgagtac tttaaattaa tcgatggcgt tcctgaagag
      241 aactctttct tgtctttgcc aaagaaagga gagaaaccct tattaacgtt gtttacttgt
      301 gtttatacgt cacaaggaaa agaaagatac gtggtacagg gagaactaca ataacaaggt
      361 tttaatcaac gtgagtcgtt gaaatatgaa atgaggagag taaaaaatgg caataaatcg
      421 gtatgtaaaa aaagctggaa tgcttattgg aatgttagga atcattgcag gctgctttca
      481 gggatatcga gcatatgcag aagatgtcac gcaaaaaacg ccaccagaga aggtcaatat
      541 cacggttcat aaattaatgt acgatcaaag tacgcaattg aatgttgata ttgacgggat
      601 caaaaatgat ggttatacgc acgatcagta tcctacaggt gtaacgaaat acaacaaggc
      661 agattatggt gatgttgagt ttacgttagg aaatattacg gatcaagtat taccgactga
      721 agattctgat ctaaccaacg cgaaagtgga cgaaatcgtg aaagacgtag aagacaaggg
      781 atctaacagt gagtacgtca agaatgcaac caataaaatt acttcagctg tagatgaaaa
      841 tggtgagatt acttttgcgg atcaaccagc gtacgtgaac tcaaaaggaa atgtgtatgt
      901 tatttacgag agtaagtccg cagcaggatt agtaactcaa aaagcaaaac caatggtggt
      961 cattgctcca atgactgaca acacaggttc aggtttttta aaagacattc acttatatcc
     1021 taaaaatatt gtttctaagt tgagttttga attaaccaaa ttcggtgatg atggtacaga
     1081 gcaaagcaaa caaacccctc ttaaaggcgc taaattcgaa ttgtataaag gagaacctgg
     1141 aaaaggaagc aaactaggcg atcttgtttc ggatgatcaa ggaaagctga ctgctactga
     1201 tttgacgtta ggaaaatact attttgtgga agttccttca gaagtcgtag tgggatcaga
     1261 taaagaacca acagccgatc aatatttatt aggcgcagac gctcgcaacg atgcgcacaa
     1321 taaattaacc tttgaaatta cgaatgatgg cgtgacaagt gacttaaaag cttcatacgt
     1381 aaactataaa gcaccagtga tcgataaaac agtaacaaat ggaacaggac aagaacattc
     1441 tttccaagtt ggtgatgccg ttaattatca aggaacgatt catatcccaa cagacattgc
     1501 aggcggagca gacggtatca cagtaaacgg cgtgaaaagc gaaacttctc cttacagtgt
     1561 atttaaatgg ggggatacag caggacaagg actttcttat gtcgctgcta aagcaaatat
     1621 caaagtaaca aataaagatg gatcagtggt attaaaagaa aatacagatt ataagatcca
     1681 aaattcagaa aatggctttg tgatcgattt tatcgtaaat aatggccaag taagtgatac
     1741 tgtagcaagt ttacacggac aagatcttca aatgacttat aacatgtatg taaatgacag
     1801 cgcagctgtt gccaatccat taacaaatag cgtggatttc gtttataaca acaatccatt
     1861 taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt gtgacttatg gcgcgaaatt
     1921 cttaaaagtc gatagcggac tatttggaac aggaattaag gcaactcctc ttgaaggcgc
     1981 agaatttgca gctaagaatg cagaaggtaa gtactacggt ggacttactg atacagataa
     2041 agatggtgtc aaagaagcag tatgggtaga cgatgtagca aatgcagcaa tcctaaaatc
     2101 agataaagag ggtcattttg aaatcactgg tttaactgaa ggagattact cactggaaga
     2161 aacaaaagct cctgaaaact atcaaaaatt aacaaaagag atctctttca aagtagacaa
     2221 agattcatac aaagaagaaa accgaatcac gatcaaaaat aatcaaaaag cttccgtacc
     2281 aatgactggt agcaacggtt tccaatctta cgtattgatc tcttgcttgt tgcttggggc
     2341 aggcgcaatt tctgcagttg tttactttaa gaagaaagcc tagaaggatc agctaatgat
     2401 tatcagacac cctaagaaaa agcgaatcat gggtaaatgg atcattgctt tctggttact
     2461 ttcagctgtt ggtgtcttac ttttgatgcc tgctgaagcg agcgtggcga aataccaaca
     2521 aaatcagcag attgccgcaa tcgatcgaac aggaacggca gcagaaacgg attcttcttt
     2581 ggatgtttct aagattgagt taggcgatcc tgttggtatt cttacaattc caagtatttc
     2641 tttaaaacta ccaatttatg acggaacttc cgacaaaatt ttagagaatg gtgtagggat
     2701 tacggaagga actggcgata ttacaggagg aaacggtaaa aatcctttaa tcgcagggca
     2761 cagtggactt tataaagata atcttttcga tgatttgcct tcagttaaaa agggagaaaa
     2821 gttttatatc aaagttgatg gtgagcagca cgcttatcaa atcgatcgga ttgaggaagt
     2881 gcaaaaagac gaactacaaa gaaactttgt cacttactta gaaccaaatc ccaatgaaga
     2941 tcgagtaacg ctaatgacat gtacgcctaa ggggatcaac acacaccgtt tcctagtgta
     3001 tgggaagcga gtaaccttta caaaatcaga attaaaagat gaagaaaata agaaacaaaa
     3061 attatcttgg aaatggcttt taggaagtac tgtttttctt tcagtcatga tcattggttc
     3121 gctatttgtt tataaaaaga aaaaataaag gagggtaacc aacatgagaa atagaaaatg
     3181 gctatttgtt tgtgcagccg ctcttttttc tttcactgcc tacacacagt tgacgtttgc
     3241 agacagtcaa gaaagcacgg atagcacaga acaaacaatc caaacgtcag atagcagcac
     3301 gattcaaagc agcgatgaaa ccgtcgattc gtcaacagtg gattcttcag aagaaccgaa
     3361 aattgctgaa gacgatcaaa tagtcaaaca gaccattcac gttcaaaaga ttatttcttc
     3421 agaacaatta gacaaccagg ggcatgaaaa acaagaagga gtaaatggcg cgaagttttc
     3481 tgtttatgat gtatcggaca ttttacaaaa gatggatgtt aaggatctca ctactgacca
     3541 aattgaatcc caattaaaag atcgagtgaa aaaactatcc agtgatcaat tgaagctagt
     3601 ttctaatgga gaaacaaaga caattgatca acaaacaggt gtctttgaat tctcggtaga
     3661 agtacaggca aatcagaaac aagcttatta tattgtcaat gaatcaagtc ctgaaaacat
     3721 ttctaatagc gaggatattt tgctattaac acctgtttct gaaaaaaacg gtgaattctt
     3781 aaaggatgtg tggatttatc ctaaatcaga agcttcgcaa cctaaagaag aagtaaaaaa
     3841 aatcgtttct actggtgtaa agaaaaactt ctttgagaat tgttgggatt ttgttactca
     3901 cttgttttct agagattaag gaagaaataa gatgaaactg atcaaattat tattattcaa
     3961 tctttcttgt ttaaccgcag tctttttgta ctgcggttta ttattttctc aattatctat
     4021 tttgccaagc taccaactat acctaatgag gattttaaac ataccagctg cttccatgcc
     4081 gctagatatt atggaaaagc tcattaggaa tccattattt actacgatca atttgatcgt
     4141 tattgtcgga atttcactag tcatttataa aaaagagggg atgaagggat gaattttaaa
     4201 cactcgtgga ccagacggtt aaaatatgtt tttcccataa tgatgatgtt aggtacattg
     4261 tttgggttaa aaacagccaa tgtctttgct gaaaaagtta ttgtcgatcc gtcaaatcca
     4321 atccagaacg taaagaataa ttttattatt ccaaaagatg ttcctaatgc aaaaacaaat
     4381 attactgtaa acggagcgtc caaagtttac aaatatcatg ttgatgccaa caaaggtggt
     4441 tttacagacg actacgtagg attagtggag ggaaacaaaa atatacgcta tccttctttc
     4501 caaaaggaat acggtgaaac aaaccttgtt ttagaaggcg taacaaccaa caccaaagtc
     4561 aatattgact atggaaaaat cggtacgtat aacgggaaaa aagtcaatat caagctagtt
     4621 ctatctaata ttcacttata ctcggatacg ttgccgtgga atctactaga taacaactat
     4681 acaaaaactc attttcgaga cgatggctat aaaaatacga atggtgcaat gagtaaatct
     4741 aaaaaaagaa cagtactttg gatctcagac aatcttttct caggaattgt ttaccattca
     4801 acacaaatga atgtgcagct agtagccaca tatgaggacg gtagccccgt tcaattttca
     4861 ggagatacat ttatcagttt taactctttg aaccctgcag gaggaaaaag cacggatctt
     4921 aaaggagaat atgcccacta tgacaaaatg aatacgacag attggtatgt cagacaagat
     4981 gcggttcttt cagaatttaa atctttctac aacaatctga acgtggtagg tggccaccca
     5041 ggaggaagct ctaagctgac acaagcggat aatgatttca ataatttgca tgataaatta
     5101 ggagatccga agtttggtca aggtactgta tcctttaaaa tatctgaagc aaatccgacg
     5161 tttgtgattg gttcttctaa tgttcaaacc tggtttactt tatcttctgc gacaatcttt
     5221 agtgttgttc ctgatcaacc tgaaaaaaca ggggttgata aaaatggaaa taacgtgaac
     5281 gataaaatgt tgcaagtagg agacacgatt caatatcgaa tcaaacagaa ggtgaatcga
     5341 ttaggagtgg atttactagc ggtttacgat cgatttgagt taatcgataa tttaccaaaa
     5401 gaagtagatt acgtcgatgc acacgttgaa agtggtacaa ataaaaaatt cgatgtatct
     5461 ggtgaagtga cctatgacaa aacaaaacac caggtaaaat atgcagcgaa agctgataca
     5521 ttaaaaaatc gaatgaagta taacggcgaa acttacgaat tagtgatcaa tgtaaaagtc
     5581 aatgaattgg ccaatcaaaa tagtgtagcc aaaaatcagg gaacgtcgat cattaataaa
     5641 gtagaaaagg atacaaatat cattaccgtt tatttcccta aaattccagt gaaagaagta
     5701 caacaaaatg gaaaagatgt aaacggacga aatgacggta agaagggtac gccgacagct
     5761 cctttaaacg caggatctga agtacaatat ctagtcacac aaaaatggca cacaaaaggc
     5821 gtagacgctg cgtcagatca ctacaaacaa tttagtattc aagatccaat tgaagctcgt
     5881 ttgacatata aagaaggttc tgcgcaagta atagataaga gtaaaggaaa agatattact
     5941 tctgaaggca cactaaccta tgatagcaat tcaagaacgc tgaagtggga agcttccgct
     6001 gacttcttaa gtaacaatct tttagatgga cgagaaattc agctgatatt tacagctaaa
     6061 actccattac aatcagaaaa aaatattgat aaccaagccg tagtagcagt agataatgtt
     6121 tctaataaaa caaatgttgt gacaattggg gttgatccta acttgccaca ggtcattgtt
     6181 cctaaaacag gttctacaca tttagtaacg atttcagcgg tcagcttact attacttgtg
     6241 ttagcaactt ttagttatgc ggttctgaga tacatttaaa ataaaaaaac gaaagaatga
     6301 ataaaatgaa aaagttaata atagtagtta cttccttgat tgccatgatt gttattttag
     6361 tcggatgtag taatgaaaaa aataaacaaa ctaatcaaga taatggcgta ttgaaatcag
     6421 gaactatgtg gaaagaagaa gtaggtggtc ttgtttataa tttgaaaatt atagacgaga
     6481 ctacctggga atactctgag agtgtatggc atccagatcc tgtacaaata acagtcaaga
     6541 ggcaaaaaga ctataaaggt ttagaaagat acaaaatagt agattcagct ggtgttaggg
     6601 aatttataaa caaatctgat tctttattta ttgttgttcc ctatgagaag aatggcgtaa
     6661 aaaaaataat ttttcttgga tctagcaagg atgaaaagcg aacgaaagag aaattgattc
     6721 acgatggatc acaatccaat aaatataagt tacaaaagac tagcgagtga ttgctagtct
     6781 ttttatttgt cctgaaggag gaatcacagt ggcaaaagct aaaacgccat ttgaaaaagc
     6841 cgcgtctaaa atcttagaac gccgcggtgt atctgaaaca cagtggaaac aagaagtcct
     6901 tgaaaaagca caatttgacc tattacgggg aaaagacaag gacttagaag gatatatcta
     6961 tcaacgtgaa cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa aggagcgaac
     7021 aattttatgt ttgaatcaat tacaatgaat tttttaacac aaccgaaagt agtagctgcg
     7081 gttgatattt ttagagcatt acaaactgcc gcaactagtt taaaaaacaa tctgatgatt
     7141 tttgctgctg ctgcggccgt tgcttccatt gttattgggg gtcttttctt cctatttgga
     7201 cgaagaggag ctgaaaatgg gaagatgcac gtaggctatg tattgatggg ggtaggagtt
     7261 attttagcag cagcttctat tgtggtttcg ttatttggat tgtttggaca aacacctcaa
     7321 ggattataag aaagtaaaaa agtaggagta agaagtagtc gatacttttt actccttttt
     7381 tatgaaagga gaatactcat gtactttacg attgcaggca ataccaaaac agataaagag
     7441 ttaataaaaa aagcaacgaa ggaagtagga aagcgtactt cttttttacc gaaaacaatt
     7501 aaatattctg tcattgaaac ggaagaacag ctacatgtgt tagggaaagt actggaacac
     7561 gaaagtcgat cttctgttaa gttgactctc acgttacatg acacagaagg aaaaatagag
     7621 aaagctggta cagaatacgt ttttggccca ctggattatc aaatttcaga aggtttctat
     7681 cctacattga tggatttaat ccgccttgat gtgtttaaag aaacggatga aactatgagt
     7741 gtggaagagt cttatcgaaa cgaaaaactt ttacaagttt tagaagaaga gttagagagc
     7801 gaccaaagaa aagtgaatca agggagtgaa gtcgaagtaa atgaagaagt accatgggaa
     7861 cgaccaatat ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa
     7921 gctgatattc cgactcagaa atcgatattt gaggaagaga acgatgtgat gggtcaattg
     7981 gaggtagatc cctttgaaga aacaatggag gatgaagaga cggccgaagt agaagttgat
     8041 ccagttgaag cgaatgaaga gtcaacatgg actgaagggg attctcagga atatattgaa
     8101 ccacttatca gtgaagaagt agaaaaaaac gaaatagaga tacaaaaggc aactgtattt
     8161 gattatattt tggatcaata tccgccagag catgaatgga tgaaagaaaa aaacaaatta
     8221 tttttaaatg agctttattc gaaatatcaa ttaccccaaa cctatcaagc atttttagag
     8281 agtaaagaag aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtc
     8341 caatcaacaa attggcatga gcttgcacaa gaagatttat tagccacctt tgaagaacgt
     8401 caacaagaaa cagatcaaga aattaatgag tatgcgacgg aacagtcgtc taattgggag
     8461 aaagcaaaag aggaactaaa taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa
     8521 attaaagagc gatacgaaaa gaaacgcgac aatgcgtatt ccttgtgtgt agcaaagatc
     8581 aataatttta ctcagacgaa tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa
     8641 gaacgtatta aagaacgaaa agaagaattt taccgaggtt tgcgtacaga taagctaaag
     8701 attagcaaag aattaaatga acaactgaat aagctatatc aaacaacgac tgaagttctt
     8761 atggaaaaat acgaaaagat ccagcaagaa ttacaagtgc aaattccaaa atggaaaaaa
     8821 gagtatgaaa ttcaacaaca agaattgaaa gaacaagaag aaaaacaacg gcaaaaagaa
     8881 aaagagcgtg aacaattaga aataagaaaa cgggaacagg atcttgcgga acaaaaaatg
     8941 gcgatagaaa aacaacggtt agagcaagaa aaggaaaaag aagcagctgc tcgtaaggaa
     9001 cgtcaagaac aaatggatac attgcgacaa gctcagttaa attatctagc acaacctcaa
     9061 ccaacgcctt cagtagctgc tgttcaaacc ccaatgaaca accaaacaaa tctaactcct
     9121 tcaccacagc gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt
     9181 ctaggtggag gaacagtatt tgcttttaat catatcaatc aatcttcaca ggctgattct
     9241 gtggccacaa cgacgtcaat caaggaagaa gcccaagaac aagcaagaaa agagtatgaa
     9301 caacaattag aagaattatt gaatcgtacc aatcagtcaa caacagaatc aagtatggaa
     9361 aatagcaaag agagtgatcc tagtaaggct aaagaaggat caagcgattc aactgtacaa
     9421 tcaaacactt ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt agagaaacaa
     9481 cgagacgaaa agacaaacga ttcaatttag aaaagaatag gtatctttct catttctatc
     9541 gctatactcc ttcaggagaa gagctaccag aaaaacatga caaacgtatt tctcaaaaga
     9601 ttttttatag tggcttagct tatcttcctt taaactatgt tgttacaaga ataccagaag
     9661 catttcatcg atttgagatt attccaggaa gtttacaagg agtgaatatt tttcagttac
     9721 aagaaatgcc gcagttattc cattttgatt caactggttt ttggaatcct ttgaccccaa
     9781 caacaagttt aggcacacgt ttagttgttt ctacgatttg tgttgttcca gctgtactta
     9841 tgggattgaa aatggacatg tggtttcgtc ctctagcaga tgggcaaaaa ggagataacc
     9901 gattaaaaac gttaaaggaa atccaacaaa tttatccgca aataccagaa tcaaagggag
     9961 gttttcctgg ttatggcgga ataccaatta ctcattataa aaagtactgg tatattcaga
    10021 cggatacagt aaatacttgt attgttggta cttcccgttc agggaaagga cagatagaag
    10081 ttttagcaac cattgacaat ttgtctcgtg cagaaagaca aagttcaatg gttgttaatg
    10141 atccaaaaac agaattatat gttgctagta aagatgaact agaagctaga gggtatgatg
    10201 tgtatgcatt taatatcacc gatccactgc aatcaatgag tgataatcca ttggcactca
    10261 tcgtgaaata ctggaagcga ggagacatag acacagcgac acagttaacc aatactttca
    10321 cgaatacgat ttattatgat gccaacgctt cagacaataa atactttaac gaaaatgcgc
    10381 aaaaagctgt caatgcgctt atttttgcgt tattggaaca agcggatcag acaggggatt
    10441 actccaaatt aacaccgaat aatctcgtcg aacttctttc tgagattggt ggttttaatt
    10501 accaagatcc tgataatgaa ttcaagcagc tcaatgcttt agatgaattt atgaatcagt
    10561 taccacctgg aaacatagca aaaaaacaat atggggcaac aaaaattggt tctgataaag
    10621 cgaaaggcaa cattctatcg acagcgatca caggactaaa tccatttaca ctgacgaaaa
    10681 ttgcgaaaat gacttctcaa tcaaccattg attataagtc cattggtttt ccgaagtatt
    10741 tggatctcaa actacacgag agtctgttaa accagcgaat tgttgtagag ttttatcatc
    10801 aaggaaaaaa atttcatgag gaacgtgtca aagtaggcta tagaggtttt tgtgaaatta
    10861 actttgattg tcatttagaa cagggagatc atgtttgctt aaaatacgat catcgaggaa
    10921 aagaatatgt tgcttggtat gagttttctc aaagagtatt gaaagacgaa gagggaaata
    10981 tcgtttatcg aaaaaaacga ggagaaaccc atttgccaga attagaaaaa gaagccgttc
    11041 taacattaga tgaggataaa agtaacttat tagtagagtc tattcggatg cactataccg
    11101 ataaaccaat ggctattttt atgctcacac ctgattatga tccatctaac catatcattg
    11161 ttagtatttt tcttagtcag ttgtataaag aattatctac gcaatgtgtt aagactaaag
    11221 gagataaatg tcaccgtagg attcatttta ttttagatga atttgggaat atgccaccaa
    11281 tggataatat ggaaggaatc atgacagtca cagctggtag aaacatgttg tgggatttgt
    11341 ttatccaatc ctatcagcaa ctttatgcga agtatgggga acaaaacgca gcgattatca
    11401 aagaaaattg tcagacacat atttatatta tgtcgacaga cgataatacc atagaagaat
    11461 tttccaaaaa agtggggaat aagacggttg aacaagagaa cagtaccatc aattcattag
    11521 gtatgaatac tcatatcaat cgcaatgttg attcagatcg catcttaact cctgaaagaa
    11581 tttctacttt tttagaaggt gaaacgattg tactcgctcc tttaaaacga caagatttga
    11641 aaggtcataa aatacggccg tatcctattt ttaatcatgg ggaaacgaat cttccttatg
    11701 cttatcaata tattgaagat ttcaatccat cgtgggatat caatgatata gacatacatt
    11761 gtacacatgc ggacttggat cttaaggctt tacaaattga ttacgattat tttctacctg
    11821 atatctatgc acgtcgactt tatcacaata tgcacgatca aatagaagag gaagctaaag
    11881 aaaacatttt tattaaaatc gttcaatctt tagacaactc aacggcacaa aagttactta
    11941 ttcaatttta tgaagagaag caaatagatc gtttgcaaga ataccttgaa aaattgcgtc
    12001 aaaatgaaga aattacagag gttataagga aacgactttt gctagaagct gaaaaggcgg
    12061 tgaaagaatg atcattggag gaatattttc cggcattgcc gactggtggt caggcttatt
    12121 tggggatggg gatgcagatc ccaaaaccgt aacgaaagtc ttacaagatt atggtaatta
    12181 tctgcagtat caagatgtat tcagttggat ttgtaatact tttttatggg gtttgattaa
    12241 agggttatat aatctcaata ctttactaga aaaaacaatt tatcaaagct ttgatttaaa
    12301 agacttattg aacgccgctg gtgttaatga actgtttcaa agtttgatta gtaaggtatt
    12361 ggctcttttt tgtgtcgtca cattaattta tttaggctta aaatttttta tgagtaaaca
    12421 tccaccaaaa atcaaaaata tcttggttaa tctatttatg gccatgattt tgatttttgg
    12481 tggtagttct ttaatcgatg aagggctaaa tattagtaaa aacttttacg gagacgtgac
    12541 tgcagcaaat aaagcagata aaactggttc acctgctttt caaattattc aaaataatgt
    12601 gattgatgtg agtacgttac tagaaaatga tccgaacaaa gtagaacaca taccaacaga
    12661 caaaagaaat gctctcacgg cgaaaaattt taaaacagcg aatatcaatg ggattattac
    12721 acctgatcaa gctaagaaaa tgtctgaatc aagtgatatt agtacagtac aaaaagatcg
    12781 gttaaaggca ttacagtatc gactagagtt agacgataat gggaaagaaa tccctgttca
    12841 aattagtgat gaaggtttag caaaatatgt gtatacttca ggctatcgcc gatacattag
    12901 tagtccaggt attattttag ctggtgaatc ttctttgtcg gtagcctatt tatttatctt
    12961 gtttacgatt gttacttgca ttattgagct ggtatttaaa aaattctatt tagtgattgc
    13021 cgcgtcaacg gactttgaga caggacaacg gatgaaaagt gctatagaag atatttctca
    13081 atccttcttg ttattggcat ttacgatttt agaattacga atctatacat taatgctttc
    13141 aggtatcggt gatttgcatg cggcaggaaa gatcaatgga ttcttgtatg ttgtcgcttt
    13201 aattgtatta accattgcgc tattcaaagg ctctcaatca gttacgaaaa ttttcggcgt
    13261 tgatacgagt ctgaaaaacg gcagcaattc tcttcttagc atgtttgcct taggaaatgt
    13321 tgcgaaaaat gtgggtagcg gggctaaaaa ccttgcaagt gctggtaaga gtggtttaag
    13381 caatttaaat gatattcgta aaaatggatt tggtaatcga aaagatgtta cccctgataa
    13441 cggggaagaa caatcagacg ataaagctac aactgtacct aatagtaaga aaacagtttc
    13501 cgaaatgttc gggaaagcga aaaatggtgt ttctaaagcc gcagaaggtc ttggttacgt
    13561 caatgaacgt gggttaaaag gaacagctga agatcttaca gaaaaagcca aagacacctt
    13621 agaagagaaa ttagacggca cgagagctaa agaattggct gaagctgtga agaatccatc
    13681 cgaaacacta gaaaaacaaa aagaaaaagc ttcagaaatc aaagaaggac taaaagatgc
    13741 gtatggtgaa ggacaagtca atgcagcagt aaaagctaat acaacaaccg aaaaagaaaa
    13801 tcaagaaggt gcagcgattc cttcagccaa agatcaccaa gatgagttgc attctaacga
    13861 aaattcagaa gaaggacaaa atggtcaatt accaaaaaca gatccagaac aatatcgtta
    13921 taaatctaac attaattctg aaaatgaaaa gcgcatttca gatttaccaa aacgaaatca
    13981 agaagtacaa acggatgatg attccatcga tctgccaaat gataatggca tcgaagttgg
    14041 aactgaaaaa tcaacgaaaa ttggtttgcc taagacacaa aatttaccaa tgaaagatag
    14101 tcgtttgaat caattcaaag agaatgatca aagctttgtt catcaatcac ctgcaggtac
    14161 tgttcaaatg aaatcaagtc gtttgcctga aaatgggaat ttgacaactg tttcttacaa
    14221 tttatctgaa agtggtgaga cagagaatgc gtccgttcaa aatattgtga caagagaagc
    14281 tgaaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg tggcatttac
    14341 tgcaccgaca aaaataaatg tgacaagaga taccaaaaac aggaacaatg cattgaaaga
    14401 acaactattg agggatatca aacaggatat caaaccataa aaggcctaca agttaggcct
    14461 tttctttata tgaaaaggag aaatcaatta tggataaata tataaacatg aaaaaccctc
    14521 caagatttag tggagaatgg aagtttttcg cttttttctt tttagatcta ttaacgcttg
    14581 gtggctgctt tttttgggcg aaaatggtca ataatcttgt ccacttacca ctcttttttg
    14641 cactcttgaa ttatggctta tggattgcga tagcgttagt gattgtttgg cgaccctata
    14701 ataacccagg tcaacgacaa atttttgcga ttatcgagtc tatttttttt gcggacaaga
    14761 accattatca tgcgttagat cctaaccgaa attatcatgg tacgtataag gaggatgaat
    14821 aaatgcgagt caaaggacaa aattcagttg ataagaaatc tattttgcag tttggaaaac
    14881 cagaagaacg atctatcttg gatttatttc cttataaatc ctgtacagaa gagggagtct
    14941 tagttactaa agaagatcat tttcaacgtt attatcgtgt cgtttccact gacgtggagg
    15001 gattaaatga acaagagaaa ctggaacgaa tgaatcagtt gaccaccgtc atgcgtacgt
    15061 atgtgccaac aattaagtta gttagtttga ctacagagac ggatctaacg gaacaaatcg
    15121 tgcaaaagcg gcagttatta gataagaatc gtatggaaca atccttagga agaaatctac
    15181 aacgattaaa aaaatatgaa aagaaattag tcgaagaaat ccaggaattg aaacgagcag
    15241 aacaagaacg tcctgactta agcttctttt tcgtcattga aggaaagaca atcaaggatt
    15301 tacaaaccag aaaccgtcag ttaatgcgat ctagtggcat tttagggtta aaaccactct
    15361 acaaaaaaga attgatcaag attctgtatc gaatgaacaa tatgaatgat gagtaggaat
    15421 ggagaagaga gaatgaaata tccattgata tggttagaaa aagatgttcc gaaggaaggg
    15481 accactagaa aatttcctca cactttaagc ctgggtaagt tgggaaaagg gcaatctttt
    15541 aaccaagtaa agaatcaaac aagaagaggt gactgtacat gttagaagca attaaaacag
    15601 ctttttcacc acaaaatgat caaaaagtac ttgatccatt aaagcaacta ggaaatcggg
    15661 aaatagaaat tttgaaatcc aaagggtatg atttagattt tttaaaacaa gtgatgccac
    15721 aaggtgggtt aaattttgat gaagaatatg tgacgtatgg taatggttat tcacgatgtg
    15781 tgacagttta tcactacaca aaaaatccta cgcttttttg gttggccaat ctcatgaaca
    15841 atcctcatac agtggccaca ctcgatgtta gcacagatga aaagcaaaaa gtgattgatc
    15901 aaatcaatcg tgccttagat gaattaaatg atcgatcaga taatgaacga aaagcgactg
    15961 accgaaataa aagcgcagaa gaatacctgg aactagcgga atatgctcgt aaattgactt
    16021 ctaatgggga aatttctaag caagtcaaag tgagagtaca tgtttacgga aacacgattc
    16081 aagaagtaga tgaacgagcg caggaactga ttgaaaattt aaaagggatg gattatcgtg
    16141 cagtgaccta tctgcacaaa acgaaaagag attggcaatc gatgtttacc atgttcgatg
    16201 aacaagctga atggtttgga aatatcaaag ggcaatcact accatcgtta aatattggcg
    16261 gaggattccc gttccaccac caaacgctaa aagatccagg agggactcaa tatggggaaa
    16321 ccatgacagg tggcccattt gtctggaatg cgacatataa aaatagtgtg cggttgtcta
    16381 ctaatacctt gcttctagga atgatgggag ctggtaaatc aaccgttctt aaaatgatcg
    16441 cggaagccca tttagctgca ggagacttta tttggggctt tgaaaagggg aaagacttta
    16501 tccccttctt aaaggaatat aacggcatta tggtgcgctt agacgggtct gacgggatga
    16561 tcaatccttt agaaatattt gccacacgga cgtatgatga agcatccagt ttatatgacg
    16621 atggatcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaaca cacttagaca
    16681 aagtagtcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg aaagcagaat
    16741 ttaagaccta cttaaatcgt ttttatgaag agtatggaac agtaccacgc ggctttactt
    16801 cctcaaattc gcgttcaaat actgagacgc aagtcacagg gaaagatcca gaagcttatc
    16861 cgacgttcaa agaattttta gactttttag gacaactgga attaccaggt gcttcccaag
    16921 agaaaaagaa tcgaaaagaa gaaatggaat ccatcgtaga atccttatgt gagacgtatg
    16981 gcatgatttt tgatggtcac agtacgattc gacaccttga ccaacaacag ttggtctttt
    17041 ttgacattga ctctattaca ggcttagatc gaggggtaca acaatgtcaa atgtacatgg
    17101 ctatgacgtt gatttggaat caggcattga ttcaaggaag aagacaacga cgtttactta
    17161 aagaaaaaca aatggaagaa caagacttca aacgcttctt ggcgatgatt gatgagtgcc
    17221 a
//
LOCUS       DACEOE010000043        17165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEOE010000043 DACEOE010000000
VERSION     DACEOE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.045"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(151..2058)
                     /locus_tag="IW774_001219"
     CDS             complement(151..2058)
                     /locus_tag="IW774_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1153573.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2188..3063
                     /locus_tag="IW774_001220"
     CDS             2188..3063
                     /locus_tag="IW774_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1153574.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3143..4117
                     /locus_tag="IW774_001221"
     CDS             3143..4117
                     /locus_tag="IW774_001221"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1153575.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4306..5442
                     /locus_tag="IW774_001222"
     CDS             4306..5442
                     /locus_tag="IW774_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1153576.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5579..5923
                     /locus_tag="IW774_001223"
     CDS             5579..5923
                     /locus_tag="IW774_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1153577.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6194..7480
                     /gene="efmA"
                     /locus_tag="IW774_001224"
     CDS             6194..7480
                     /gene="efmA"
                     /locus_tag="IW774_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1153578.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7618..8217)
                     /locus_tag="IW774_001225"
     CDS             complement(7618..8217)
                     /locus_tag="IW774_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1153579.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8621..9742
                     /gene="mnmA"
                     /locus_tag="IW774_001226"
     CDS             8621..9742
                     /gene="mnmA"
                     /locus_tag="IW774_001226"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1153580.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            9988..11409
                     /gene="arcD"
                     /locus_tag="IW774_001227"
     CDS             9988..11409
                     /gene="arcD"
                     /locus_tag="IW774_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1153581.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11423..12541
                     /locus_tag="IW774_001228"
     CDS             11423..12541
                     /locus_tag="IW774_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1153582.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12601..13893)
                     /locus_tag="IW774_001229"
     CDS             complement(12601..13893)
                     /locus_tag="IW774_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1153583.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            14043..14603
                     /locus_tag="IW774_001230"
     CDS             14043..14603
                     /locus_tag="IW774_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1153584.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            14732..15109
                     /locus_tag="IW774_001231"
     CDS             14732..15109
                     /locus_tag="IW774_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1153585.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15042..17114
                     /locus_tag="IW774_001232"
     CDS             15042..17114
                     /locus_tag="IW774_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1153586.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         5486 a         3107 c         3658 g         4914 t
ORIGIN      
        1 gaaactaagc agctttttca caacctctaa tacatggtat tccagaagta acttcttcga
       61 aaaataagcc gaaatgtcac aaaaatttga gaagcgattt tcccaaattc cttcttattt
      121 cttgaagctg accacttctg gcacaacctc ttaccagatt cgcactcttt tttctggcgc
      181 tagatacata gcgtcttgtt ccgtgatacc aaatgtttca tagaattcgt caaggttttt
      241 tggttgcacg tttgctcgga gtttagcagg ggcatggaca tcgatttgca gtaaaagttg
      301 ttggtattct ttttttgctt ttgtacgcca aatcgttgcc caattgacaa agaattcctc
      361 gatagaaaga tctgattccg tttttgctgc ttccaatgca cagcttagac cgcctgcatc
      421 agcaatattt tccgaaacgg ttaattttcc gtttactttt ccgtcagcaa aaggcagacc
      481 atcgaattct tcgatcattg cttgtgcttt gttttgaaat tgtgtaagat cttcttctgt
      541 ccaccagttg ttcaagttgc catattcgtc aaataaagcg ccgttattat caaatgcatg
      601 ggaaatctca tgagcgataa cagctccgat tccaccataa ttagcagagc ttgattgttc
      661 tagactgtaa aatggtgctt gaaggatcgc tgccggaaag acgatcacat tccgaaatgg
      721 atgataataa gcattgaccg tgtcagcact catttcccat tctgaacgat ccactggttt
      781 gttccatttg ctgaaacgat cttttaatgt gagtttgctg aaaacaagag tatttgaaag
      841 caaagtacca ccctgatctg caggtactgt tttgaactgg tcgaataatg ctgggatctt
      901 atcggggtag cctacttgga tacctaagtt atttaatttt atgacggctt tttcttttgt
      961 tgcatcccca agccagtcat tttcttctag acgtttttta taaacggcga tcattttttt
     1021 caccatttgt tctacgtctt ttttagcagc ttcgccaaaa tatttttttc cgtagtaatc
     1081 gccaactact tgatcgaatt gtccagaggc aaggtaatac gctgcttttt tctgaggcat
     1141 tgcttcgtct gttccagaca atacgcgaga atagattccg ctgacttggc ggaattcttc
     1201 tgatagataa ccactaagag aacgtactgt tttaatgatc atccagcttt tgaataacgg
     1261 aaaatgagct tctgtcagga tgtcattcaa atgatcgaag tacactggat cagttacgat
     1321 cactttatct ggagttgtcc caatcaaacc cgtgacaagt tttttcaagt ccagtttatc
     1381 tgtgtattta ataaatgtat caaaagattg cggattatac attttgctgt aatctgcgtt
     1441 ttcttctgca ctttttacat gaggagcaat caatttatca aattgcactg cttgttccgt
     1501 gattgcttct gcctgacttt tttctttgcc ggcaagctct aataactgaa cagccatatc
     1561 aaagaaaacg cctattagtt gtgcaccgtt gggatggtct gatgcataat acgtcttatc
     1621 tggaaggaat aaagaaggcg gataagcaaa gaaggcgttc gtttgcgcat tcttcatatc
     1681 tgcatctaca tccagattaa atggcaatgg taatgtatcc aacagccatt ctggaaattg
     1741 cttattcaat tcgtcgaaag actgcaatga ttcaactttt tcgattatag gttgaagcgg
     1801 ttgagcatgc aactcatttc ttcggtcata atcgtttgcc agttgataat atgcaaggaa
     1861 atgtttcatt ttttctgaag acactttttc tggatgggca agcagatcgt ctgtttctcc
     1921 catcaaaagt tggtcgatcc cgtcaactaa atcttggaaa ccgcctgtag ctggtttatc
     1981 tgcagggatt tttgctgttt tttgccattc ttcattgact gcttcataga aatcttgttt
     2041 caatcgttct ttgttcattc atcgatcgct ccttttttgt ttcgtgtgcc cattatctta
     2101 caaaatgaag gaattaacaa atagaacata aaaaattgaa aattcgaaca atcaaactga
     2161 tttgtttttt tcaatccttc acttataatg aaaaaaagga ggcgatccca actgaatcaa
     2221 gtagtggata aacctttata tttcaaagta gataaaaaaa ttaaaaaatt agccagcact
     2281 cagcagctgc agtcaagaaa aagtaaattg ctttttttag ctctggtttt tgaagatcaa
     2341 agttatgtga ttatcgatca atcaggacat ccggtagaat attctccagc aaaatatact
     2401 tatcaagagg gtctttcttg taaaaaatgg aagctgatca atgaagagcc aatcgagtta
     2461 tctagatgga tcaacagaaa agaagatatt cccgttttga tcgaagaaaa aaggtcagga
     2521 aaggaactcg caaattgctg ggtgggattg ccagaagaac gttttttgag gtataagaaa
     2581 tgggctaccc cctcaggcta tctttgtggt acttacgcgg cagcagttct ccttgcctat
     2641 tatcaagact atcgtaaggg gtggatgctt ccaaatgaga ttcgcaagaa aaatacggct
     2701 gattcgttgg ttttgacgaa ggcattgcgt agccagatcc aaccactagg acttcctacg
     2761 attccttttc aggttagtac agggatttct agttttttaa agaagaatgg caatcatgag
     2821 cgtgcacgag caactcttct aggttcatgg cagcgagcta caaaacgtat acgtgaaggc
     2881 aaacctgtga tgattggtat tttgaaagta cttggcagta catatggcaa tcattgggtg
     2941 acagcgtacg cttattttga aactgaaaca ggagaacgtt attacaaagt acacgacaac
     3001 tggggcgatt atcataaagt cattcctgcc agttggagta acggaacagt ttcacttcct
     3061 taagaatttg ctgaaattaa cagaaaatct tggaaaaata tgttaaacta tcgaagaata
     3121 gagaagtttt ggggaggact acatgagtaa taagtatcag gatgacacgt tgaagatttt
     3181 tagtttgaat ggcaaccgtc cattagctga aaaaatcgcg aaagtgttcg gaacagaatt
     3241 aggtaaaagc gtcgtgaagc aatttagtga tggcgagatt tccatcaata tcgaagaaag
     3301 tattcgaggg gatcatgtgt atatcgttca atcaacgaat gcacctgtca atgattacta
     3361 tatggaacta ctgatcatga tcgatgcaat gaagcgagca agtgcgaaaa cgatcaatgt
     3421 cgtattgcct tactatgggt atgctaggca agaccgcacg gcgaaacctc atgaaccgat
     3481 cactgcgaaa ttgatcgcta acttgatcga agaagcaggg gcaactcgag tactgacttt
     3541 agatctgcac acggttcaag tacaaggatt ctttgatatt cctgtagata atttatttac
     3601 gatgccgttg tttgctcatt attaccgtca attaggtctg acgggagatg acatcgtagt
     3661 tgtttcgcca aaaaacagcg gtgttcagcg tgcacgtagt ctttcagaat atttgaacag
     3721 cacattagcc atcgtagatc atgcagatga tgcttcaact gagggtggtt atgtgatcgg
     3781 tgatgttcaa ggaaagacat gtatcatggt agatgatatc ttgaacacag gagcaacgct
     3841 tgcacgagca gcaaatgtat taaaagaaaa cggcgcaaaa gaagtatatg cctgtgcgtc
     3901 acatggatta ttgtccgatc atgcgaaaag tattttagac aatgcaccaa tcaaagatat
     3961 ttgtatcact gattctgttt tcacagaaga aaatcgtcat ccagaaaact taacaatcgt
     4021 tacatgttca gaattaatgg gtgaagcggt caaacgcatc catgaaaata caccgatgag
     4081 tccgttattc cgtctggaag aaaaagaatt cgaataaaca aaaaaaagag ggaaagaagt
     4141 tgtgacagaa gtgagcagct tcaaggagct gcgacaagac ttttgtcaca gctccttttt
     4201 tctatgccca tgaaacgtcc tctagacaaa aatgagctct tctttggttt gttcgctatt
     4261 ttatgctaga atgtgcctag caataatttg gaagggatga atgaagtgaa aactatttat
     4321 ctggatcatg cggcaactac ccccatgcat ccacaagtaa ttgaagcgat gacggcaatc
     4381 atgcaagaca cgtttggaaa tccttcaagc atacatggat ttggtcgcca cgcgcatgaa
     4441 aaattggaaa cagcaagaca aatcattgca gacagtctgc aagcaaaacc tcacgagatc
     4501 atctttaaca gtgggggaac agaaggggat aatacagcta tcttagaaac agcgttctcg
     4561 cgtcaaaaag aaggcagaca tctaatcact acagctatcg aacatcctgc tgttcttaat
     4621 accatgaaat atttagagac aaaggggttt gacgttacat atctgcctgt agatgaaaaa
     4681 gggaatctgt cgatcgaaga ggtcaaaaaa gcgttacgag aagatacgat cttggtctct
     4741 gtgatgtacg ggaacaatga aatcggaaat ttgatgccaa tcaaagaaat cggtgaactt
     4801 ttagcggatc atccagctta tttccataca gatgcggtac aagcgtacgg aaaccaaacg
     4861 atccatccag aagaattagg aatcgattta ttaagtatct ctgctcataa aatcaatggg
     4921 ccaaaaggcg tcggtttctt atataaacga gacggagtaa atatccctgc attgctttta
     4981 ggcggggagc aggaagaaaa gcggcgtgca ggtacagaaa atttagcagg gatctggggt
     5041 atggcaaaag cagttgaact gctgtcacct gaagaacgtg aagcacgtaa caacaaatat
     5101 aaaggattcc agcaattgat tcttgataca ttagaagcaa acgaaatccc ttatcaagta
     5161 aatggtgatt taacgaataa actccctcat gtgttgaatc ttcgcttaat tggcgaagca
     5221 aatgatttgc tactgatgaa gcttgatctt aatggggtcg ctatttctac tggttcagct
     5281 tgtacagcag gaaatatcga gccttcacac gtattagaag caatgtatgg agaagataca
     5341 ccgatactaa aagaatccat ccgtatcagc tttggtttgg gaaataccga agacgacatc
     5401 cagtatttcc ttgatcaatt agttcgtgct gtcaaaaaat aactagtgaa gtcattctta
     5461 tatgaacaga tgtctgagag aacgccttca ttttttaggg aacttaactg aaaaacctag
     5521 taaaaatcag gataaacttt tataataaag agaaactaat tttatcgagg tgaaaatcat
     5581 ggcatttgaa acaacagcaa cggtactggg cgcaccaatt agctatcgtt tgcatcctga
     5641 tgcaaaaaga tatacattgc gcgataatgg atttacagaa acaaacggag gcaattatca
     5701 attgatccga ccattagatg ccacgccgca aagcaaagaa ggattcaaat taaaaatcac
     5761 tgtatcaaaa gatattcaaa cattgaaaat gtcgatcact acagcaaatg gattgaaagc
     5821 agtcaatatt ttcaaagatc caaaacagaa aatgagccaa gataaatttt attttctgat
     5881 ggatgggatg attagccgtg ggttattcga aaaagtcgaa tgatctgtgg atataaaaag
     5941 acaactagtt ttgaaacggc ctattgttag atgactaact ttgtaaggac gacgcaaatt
     6001 agttgtcttt tttattttta aatttaacac atgttgttat ttttaatatt ttgctgattt
     6061 attgtttcta agtaaagaaa tttaaattga aacgattgtt atttagggaa aaaggtattt
     6121 tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg ctatacaaaa tgaaaaagaa
     6181 aagagggatg tttatggaaa atgaacagtc ggtcgtttta acgaattgga agagaaatta
     6241 tttgttcttt ttatcgggac agtttttgtc aggaatcact agtatggtcg tccaatatgc
     6301 gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca attttgagtt ttgctacttt
     6361 actaggaatg attcccatgg ttttactaag cccatttgta ggaccgctag tggatcgctg
     6421 ggataaaaaa gctcttttga ttgtcacgga tattattgta gcgattttcg cgcttatttt
     6481 agcagtagtc ggaacgattt cagaatcctt cccgatttgg ctagtttttg tgtctttgtt
     6541 tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc cagtcgatta tgccgacaat
     6601 cgttccatct tctcacataa caaggacgaa tggacagtta ggaatggtcc aatcagcgaa
     6661 tttcatcatt gcgccagctc taggtgcggc tttgttttca gtcgttcctg tcaattacct
     6721 tattttatta gatgtattgg gggcagtttt tggtgtcggc ttattgattt ttgtgaagat
     6781 cccaaaagtt tctcctgaaa tattggaagt tccgcttact atttttaaag atgcaaaatt
     6841 tgggctgcag caattgatgg ataataaagg gttatggtac ataacgatca atggtgcatt
     6901 tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca ttgatgacgc tagattactt
     6961 tggtggttcc gttggacagg cgggagcagt ggaagtagtc tacgcagtcg gaatgctctt
     7021 aggcggtgca ctgatcagtt ttataggaac atggaaagac cgaatgaaac ctattattat
     7081 agcctacatc attatgggcc tgacgatcgg tgcaagcgga ttggttccaa acgatagtca
     7141 agggttcttg tactttctta tcttgaatgc aggagcaggt tgtgcaacgc catatttcaa
     7201 tacactgctg atggcaatga tccagcagag ttatgaatcg aatgttttag gtcgtgtctt
     7261 agggaacttc aactctttga tgaacctggc aggtccaatt ggattattat ttgcaggacc
     7321 attagctgat cgtttagggg tagaaaaaat gttcttgttt tccggaatcg ggattttatt
     7381 atgcggaatc gttttatttt taacgtcagc tgcccgaaat tatgataaag aattacagaa
     7441 aaaactggta aaagaacacc atgagcaaaa agacgaatag tatggaatgg agttgtgaca
     7501 gaagtcttgt tacaactcca tttctgatcc taatgttcct gtggcaataa gcctactcaa
     7561 atgaaaaata tgagtaatta tctttatttg tttaatactt gatatagggc agttttttca
     7621 cgacctatcg tacttcctct ttgcttcata gatttctttg atttgttgct cagataacgg
     7681 agtgccaaat ggtagttttt cctttaagag ttctctttcc ggtgcgtcta cgcctacatt
     7741 gacatcgttc tcgataggta cgctgtaatg atggatatgt ccatgaagat tgcggatagt
     7801 gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa tattgttgat ggtcgaattt
     7861 aacgatggca cctacatcat aaaaataaaa tttgggactt ccgtcagtaa gcctaggatc
     7921 atttttttct aaataacgga aaaaagcgcg actgtcatgg ttccctttga tcaagtggat
     7981 tgttccttgc agttgagtga ccagttccaa aatttctgca tttgcttttt catattgcgg
     8041 atgcatagca aggtcgccta gatgataaac atggtcggtt tcttttatta ctgcattcca
     8101 gctagtgatc aactgttggt tcatttcatc cacatttgca aagagccgcg gagcaaagtc
     8161 gttttttcct aataagtgtt catggaaaaa gtgcatatca ctgataaagt aattcatgta
     8221 tcttttgcct cccttttttc ttagagtaaa caaaatcact tatctgagca actcataaca
     8281 tctaaaaaga aaaggttgta aatgagacgt gcagctttaa gcgataagaa gcaattttga
     8341 aaaatagttt ttcacaattt ataaaattat tccttattgc cgaaaggctg gcccttttta
     8401 tgagtatgcc aattttttat atcgaatgct caaaagatga gtgatttttt ggttgactat
     8461 catgtttgtg tactgaatag gtccagtaga acagtatttt tacgacttga taattaaggg
     8521 atgaaatttt cagaaatatg acgtataatg taaaatactg aaaagttgcg accttcaact
     8581 cgtagatgtt tcagaatcta attgaaatga tggtgatatc atgacagaca acagcaaaac
     8641 acgcgttgtc gtagggatga gcggcggtgt cgactcatct gtaacagcac ttttgttaaa
     8701 ggaacaagga tacgacgtga tcggaatatt catgaaaaat tgggatgaca cggacgaaaa
     8761 cggtgtatgc acggccacgg aagattataa ggatgtagcg aaagtagctg ctcaaatcgg
     8821 gattccttat tactctgtga attttgaaaa agaatattgg gatcgtgtat ttgaatattt
     8881 cctagccgaa taccgcgcag gacgtacacc taacccagac gtgatgtgca acaaagaaat
     8941 caaattcaaa gcattcttgg attatgcaat ggatcttggc gccgattatg tagctactgg
     9001 acattatgcg caagtcactc gcgatgaaaa cggcgtggtc cacatgcttc gtggagtaga
     9061 caataataaa gatcaaactt acttccttag ccaattatca caagaacagc tttcaaaaac
     9121 gatgttccct ctaggcggca tggaaaaatc agaagtacgg gcaattgctg aacgcgcagg
     9181 ccttgcgact gctaagaaaa aagattcaac gggaatctgc tttattggtg aaaagaactt
     9241 caaacaattt ttgagcaact atctgccagc aaaaaaagga aatatggtca ctcttgatgg
     9301 tgaagtcaaa ggacaacatg ctggcttgat gtattatacg attggtcaac gtcaaggact
     9361 agggatcggt ggcggaggag attcgcaaga accctggttt gttgtaggaa aagatctagc
     9421 gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct gcattgtatg ccacaagctt
     9481 ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg ccaaaagaat tcaagtgtac
     9541 agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca gttcgcttat tggatgataa
     9601 tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc actcctggac aagcagttgt
     9661 attttatgat ggaatggaat gtctgggtgg tggtttgatc gaccacgcat atcaagaaac
     9721 aaaagtttta caatacgttt aagcgatatt aaatgaatca aaacagctat ggctaacaaa
     9781 agaaacacct tttcttttgg atagcatagc tgtttttttg caataaaatt aatttttatt
     9841 cattttatac ttgaaatact tgtgtaaaaa gttcataata tccaataata tgaataaagt
     9901 tgctttttgt taagcaagag cagttgcaaa atagaaggga atcttttgca agtgatttgt
     9961 tcaattttat aagtgagggt gatggaaatg gaaaagaaat cgacgggtat tagcaaatgg
    10021 ggactggttg ctctggttgt cagttcgtca atcggaagcg gtgtgtttgg gatcacaagt
    10081 gatctggcta gttctgcagc tccagggcca gcgatccttt catggataat cgttgggatt
    10141 ggtattttag ctttggtatt atcgctgaat catttaggag aaaagcgtcc agatctagat
    10201 ggtggtattt tcggttacgc ggaagcttca tttggtaaat taggtggctt catcagtggt
    10261 tggggttact ggttgtctgc gtggctggga aatgttgctt ttgcaacgat gctgatgagc
    10321 gccatgggag aatttttccc gatattcaaa ggtggacaaa atgttccttc tattttattt
    10381 gcaagtattt ttatttgggg tctgacttta ttagtaaaca atggagttga aggagcgagc
    10441 gtcatcaata caatcgtgac gatctgtaaa ttagtgccat tattcctatt tttaatattt
    10501 gtatttatcg catttaaagt gaatatattc actgctgatt tttggggtaa cgtatctgat
    10561 aatctagtga atggaacagg gcagcaagga acgatctggt tgcagattaa aggttgtttg
    10621 atggttatga tgtgggtctt tgtagggatc gaaggcgcaa gtgttttagc caaccgcgcg
    10681 aagaaacgtt cagaagccca gcaagcatcg atcattggtc tgctttgttt attgtttatc
    10741 tatatccttg cttcgatctt gccatacggc gtcttatctc aagaagagtt agcaactatt
    10801 tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg gaaattgggg agcagcgctg
    10861 atcaatatcg gcttgattat ttcgatcatt ggttcttggt tgtcttggac gatgctccca
    10921 gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc cgaaaggttg gggaaaaacg
    10981 aataaaaaaa atgccccaac ttattcgtta gttgttactg caatcttgac taatctattc
    11041 ttattgactt tcttagttac tgattatgcc tatcaatttg cctattcttt atgtacagcg
    11101 gcaattttga tttgttatct acttgtcggg atctatcaag tccagttttc tttaaaagaa
    11161 ggaatcaaaa aacaagtagt gatcggtggg attgctgtag tttttgaatt gatgggcatc
    11221 attttagcag gattttctta tgtcctttta tgtagtattg cctatcttcc tggtttttgc
    11281 ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta cgggaaaaga aaaagtaatg
    11341 attgggctga tttgtgtggc cgcactgctt tcaatcgggt tgcttgctgt aggctggatc
    11401 aagatataag ggggaaagaa acatgaaaat cgcaaatttc ggtgtagaag aatggctaaa
    11461 taaatgggaa aagaaagcgg tatatgatat tgcacaaagc tcgatcgaag cattgacatt
    11521 agacgaactc gttggcttag atgggactag cgtaagtaaa tttttcgaaa aacgcaaaaa
    11581 cgaaccattg gactatggct ggatcgaagg atcagatgtt ttcaaacaag aagtcgcttc
    11641 tctttatcaa accgtcgatc cggaaaacat cttacaaacg aatggagcga ctggtgctaa
    11701 tcttttagct ctatacgcgt tagtggaagc aggagaccat gttgtttcga tgcttcctac
    11761 ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc gctgttgact ttgttcattt
    11821 gaaggaagaa gaagattggc agtttgattt agaacagcta gaagccttag tcaaacctga
    11881 aacaaaaatc atctgtttga atagtgcaaa caatcctaca ggcacattgc tagatcggaa
    11941 aactttagaa aaaatcgcag aaattgctcg tacagtagat gcatatgtat tgatagatga
    12001 agtatatgcg cctttaacgg ataaaggtga attcttgtcg atcgtagatg tctatgaaaa
    12061 aggtatagca accaattccc tatccaaaac ctactccatt ccaggtattc gtattggctg
    12121 gacagctact ggacctgagt tggcagaagt ttttcgaaag tatcgagact acacgatgat
    12181 ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca ttgaagaata aagaaaaaat
    12241 attggcgcga aacaaaaaga tcattacaga gaaccttgtg attttaaaac aatgggtagc
    12301 caatgaaccg aaagttgaac ttgtcgctcc caattacgtc tctacttctt tcattaaatt
    12361 agccatcgaa gaagatgacc agacattttg tatcaattta ttagaagaaa caggagtact
    12421 attagtacca ggaagtgcct ttgatttacc gaagcatgcc cgactaggtt actgttgtaa
    12481 aaaagaaacc ttggaaaagg gattattcct cttatctgag ttcttgaaaa aacaggccta
    12541 aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa gcagtgtttt tcatttacgc
    12601 ttattgtcta tgagaaggag agtgccctga agctatggag actcctgaag ctttttcttc
    12661 tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt acaagcaaga agttgaccaa
    12721 aacaagaaga gaagtcgaaa gaaagacgcc acgataacca aatgcactgg aaacactgga
    12781 gccgatcata ggccctacta cgttaccagt tgcttggaaa gattggttat aactgaagat
    12841 ccgtccagca gcttgatgag gcgaatattt tgtgatcaat gcttggacag cagggagcag
    12901 acaagcatca gagataccaa tcaaaaaacg taaggcagct aattgccaaa cattcgtgac
    12961 gaatgccatt gggatataaa ccagaatcgc aaaacccaaa ccaatcgcaa gtattctttc
    13021 actcccaatc ttgtcaccta agcgaccaaa tcttggagcg gctatcaaag tggcaatccc
    13081 aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc ccatgtccat gcagcaattg
    13141 gcgaatgtac agactgatga ttggactaat ggaattgttg gatgcttgga tgatcatcgt
    13201 tgtgacgaac ataccgatga cgacgtgtgg atatttcaag tctttgaaaa tctgtttggc
    13261 agaaaccatg ttttcttttt tcactgggac aaatttttca tggacgaaaa acagactcaa
    13321 caagaagacg aaaaataaga tgatccctgt aatgaaaaag gtagggcgat agccaaacgc
    13381 tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt cctgtaacag aaccagttgc
    13441 taaggttcca agaacttgac cacttttttc ttttggcgtc ccagttgcga caagggcagt
    13501 ggcattactg atataaccag aaaaaatccc ttgaagcaga cgcaatgcga ctaattgata
    13561 cacactagtc acgcaaccca tcaaagaaat gacgattgcc attcctaatg aagcacgcaa
    13621 cagcatcaat tttcttcctt tttggtcggc taattttccc caccaaggag agataagcgt
    13681 agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa aagttcaatt gtgacgtact
    13741 ataattacct aacgtatcta tgtaaagtga cataaaaggc atgactaaac taaatccgat
    13801 accagccata aatgttccaa accataagac aaacaagttt tgttcccaag tttctctttt
    13861 cctaaataat ttttccttta ttcttgcagc caattagact ccttctttct gaaaattttc
    13921 atctacagct ctttattata aactacaata tgtagtttat caatgaaagt ctcttttttc
    13981 tctttctttt tcgtggtaaa ctgaaagagt gaaggattta ttttatgaga ggaaacatgg
    14041 aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga ctgctttcga gaactagcag
    14101 aagagatgga tgttcagcaa ataacctttc agcatttagc caaacgcttg gatatcaagg
    14161 ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt caaaacagcg ctaacagcga
    14221 aacttctgca ggaacttaat gaccagctgc gacgggctct tgtgggaaaa agtggtcagg
    14281 aagcactgca gatctatgca gaaacttatc gatcctttgc tttttcaaat caagcagtgt
    14341 atgagttact gatcagtgta ccgcatacca atgaagctgt tttattagat ggaatccatg
    14401 aaacgaatca gatcattttg cagatatttg aggcttttca attaacgaaa gaagaaaaag
    14461 ttcatcgaag cagggaactt cgtagtatga tccacggtta tctttcctta cgttttttag
    14521 gatactttac aaaagaacca acaatcgatc cagaagacag ctatttttgg atgatcaata
    14581 actttatcgc tactttgcct taaacaatca gattcatttg taacaatttt aagatgttca
    14641 ttaaatgacc cttttataag agaaaattct tgcaactttc ttgaaaagtg actaagatga
    14701 gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa gtaattacca tgttcgggga
    14761 caatgagcct tggtggtttt ttgaagactg ggaagaagat attgaagaag aagaaacatt
    14821 cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa tggaacgaaa tccatcaaaa
    14881 ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc ttttggaatg acggagatga
    14941 gcgctggtgt gaagagtgtg acgatgattt acagcaatac aagggacttg cgttgttgaa
    15001 aaatcatcag cccatcacag tagaaagcag aaaggaattt tatgaaacaa ctaattctag
    15061 cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct tggtgctaat caaaagcaca
    15121 aaaattatta tgaaggaccc aaggtgatcg ttacttgggc ccttggtcat ttattaggat
    15181 tgaaaatgcc ggaagatctg aataaggaat ggcagagctg gcaaatggac acgttgccaa
    15241 tgatccctaa aaaactagga atcaaaccac ttcctaaaac aggacatcaa ttaaaagcaa
    15301 tcaagcagct ggctcagcga aaagatgtct cagaagcagt cattgcgaca gatgccggaa
    15361 gagaagggga acttgttgct cgctggatct tagagtatgt gcatttcaat aaaccagtga
    15421 agcggttatg gatctcttct caaacagata aagcaatcaa aactggattc aagcaactaa
    15481 gacctgcgaa agaatatgat gcattgtatg aatctgctct tgcccgggca aaagcggact
    15541 ggctggtagg attgaatgtg accagagcct taactgtgaa gtacgcggat aatttatcag
    15601 caggccgtgt gcagacaccg acactagcat tagtgagaga ccaagaaaag aaaatcgagc
    15661 agttccgtcc acaaacgtat ttcacgatca cgttgcaagc tggaaaagaa caagccaaaa
    15721 tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga agcacaaaaa cttgttcaat
    15781 tgatcagtac aagcaaagga actgtcacag aagtccaaga aaaattaaaa acggaatctg
    15841 cgccattgcc ttatgattta acagagatcc aaagagaagc caatcaacgc tatggctatt
    15901 ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga gacccataag atcgttactt
    15961 atccgcgtac agacagcaaa tatctgacaa atgacatgaa aagtacgatg aaagaacgat
    16021 tgcaagcagt cagcgacttt gctcctgagg tcaagcagta tttgaaaaac ggagccatcg
    16081 tccaacagac aaaagtattc caagacagta aagtgaccga tcaccatgca cttttgccaa
    16141 ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga gcgaaaaatc tatcaaatga
    16201 ttgtctctcg ttttttagga ctgtttgctc aaccccataa agtcagccaa acaaaagtta
    16261 cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa tcgtgtcgtc caagcaggat
    16321 ggaaaggcga aactgaaact gagacggaaa cagtcaaatg ggaaaaagga atgagaatca
    16381 atcccgattt cgccatcaaa aaagaactat ctgcaccgcc taaaccattg tcagaagcaa
    16441 gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc agctacgcgt gcagagatta
    16501 tcgagaaact aatcaaatct gaactgatgg aacgtacccc tcaaggactg caagtatctc
    16561 ctaaaggtaa gcaattatta gtattggtaa atccctcttt agtaacacca gatttgacag
    16621 aaagctggga aaaagacctt gaagctattg ctgcaggaaa gaaacaagca ggaaccttct
    16681 tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga gatcaaatca agcaaacaag
    16741 aatatcaaga tttctcgatt acccaaaaga aatgcccaga atgtggggcc aatctgcgcg
    16801 agaaaaacac acgtgacggc aaaatctatg tctgcaccaa ttcagattgc aactaccgca
    16861 gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca atgtcaccgg aaaatggaaa
    16921 tcatcgaagg taaaaacggt gcttatttcc gttgtaaata cgatggaacg acagaaaaat
    16981 tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca tgaagaacga cgcttgctga
    17041 aaaaatatgc acaagcagaa gaaccagaag aaagcccatt agcagcagca ctaaaagcag
    17101 caatgaagaa gtaataagat atgaaagagg ttgtgataga agtggtcagc ttcaagaaat
    17161 aagaa
//
LOCUS       DACEOE010000044        17058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEOE010000044 DACEOE010000000
VERSION     DACEOE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.046"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            781..1461
                     /locus_tag="IW774_001233"
     CDS             781..1461
                     /locus_tag="IW774_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1153587.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            1490..2803
                     /locus_tag="IW774_001234"
     CDS             1490..2803
                     /locus_tag="IW774_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1153588.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            3609..4376
                     /locus_tag="IW774_001235"
     CDS             3609..4376
                     /locus_tag="IW774_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ1153589.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            4403..5404
                     /locus_tag="IW774_001236"
     CDS             4403..5404
                     /locus_tag="IW774_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ1153590.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            5427..6713
                     /locus_tag="IW774_001237"
     CDS             5427..6713
                     /locus_tag="IW774_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAQ1153591.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            6747..7400
                     /locus_tag="IW774_001238"
     CDS             6747..7400
                     /locus_tag="IW774_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1153592.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            7433..8638
                     /locus_tag="IW774_001239"
     CDS             7433..8638
                     /locus_tag="IW774_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1153593.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            8768..10093
                     /locus_tag="IW774_001240"
     CDS             8768..10093
                     /locus_tag="IW774_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153594.1"
                     /translation="MIVAAMSYHLTGNSNVASLIAVVGVVYAIVKACNNEYLAIWVMF
                     VLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKVKARSRYGIVYSVMLIFIL
                     IACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSERITKNEFIDLVCKFTRWT
                     MCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGYNST
                     NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALIACL
                     VSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDARAV
                     EYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRDKSV
                     AAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            10110..11240
                     /locus_tag="IW774_001241"
     CDS             10110..11240
                     /locus_tag="IW774_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153595.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGVSP
                     YDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLKKYAVVADSEK
                     LLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            11266..12351
                     /locus_tag="IW774_001242"
     CDS             11266..12351
                     /locus_tag="IW774_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAQ1153596.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            12374..13189
                     /locus_tag="IW774_001243"
     CDS             12374..13189
                     /locus_tag="IW774_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153597.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            13173..14891
                     /locus_tag="IW774_001244"
     CDS             13173..14891
                     /locus_tag="IW774_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HAQ1153598.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            14917..15753
                     /locus_tag="IW774_001245"
     CDS             14917..15753
                     /locus_tag="IW774_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1153599.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIEKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            15758..>17058
                     /locus_tag="IW774_001246"
     CDS             15758..>17058
                     /locus_tag="IW774_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1153600.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILRENGIKEDMYNIKNIKFLSVLM"
BASE COUNT         5538 a         2366 c         3743 g         5411 t
ORIGIN      
        1 gcacataagg aggctgagtt tgttgtcatt tctgctccta caaactatgg tagtgaatgt
       61 gttattgaat tagtgatgtc tgttacttca gagacattgg ggataattaa cttcactata
      121 cctgtagggt atacaaaaaa tggaagaaaa aatattatca atagatttta tttagttcag
      181 aatttttacg tggaagtaaa ttattgatga taatctttat ccaattagaa ttattatcgt
      241 aggatgtgat gatgatagcc gtgaagttgt tcaagtattt accaatgtct acaacgcaga
      301 aaattagttt gggtttttcg gattggaaaa tttcaatgta taaaatcata aatttgctgt
      361 aaatagacag tttggggtag taatagccca ataaattgtt cgataaactc atctttgggg
      421 tgagaatagc tcaatcattg aatttcaaac ctgcctttgg ggtgggatta gaggtcaata
      481 tgtacctaca taatactttt tggaaaatga tggtgttgag aatgtaagta catttcaatg
      541 tgaaattcta atatagcccc aaacgaggtc aataaaaatg acctaaactc gtatttctcc
      601 cctaaacgag gaggagatag atatgaaaat tgaatgtgcc ggcgttggag ttggctgctt
      661 ttagtaggaa tgaacatggt tcgatgagaa ctgtttcaga tagagtggtc gatgatcaca
      721 ggcctttaag tcgtttacag atgatgagac ttgtaaaaat agagggacga atggaaaaag
      781 atgattggta tagaagaaaa gaaaggtgtt cagaaagacg taacaattga tagggaagag
      841 aatcttaggg gacatagatt ctattggatt ggacgcagag cacaagatat cttcttctcg
      901 gctctagctt tgattgcatt gagccctgtg atgctaatta cagcgctcgc cattgtgatt
      961 gacgatcccg gtggcagccc gatttttgtg caggatagag ttggaagaaa tggaaagctg
     1021 ttcaagtttt ataaattccg atctatgtgt gtagatgctg aagcaaaatt agaagccttg
     1081 ttggcacaaa acgagatgga tggtccggtc ttcaagatta aagatgaccc aagaatcacg
     1141 agagttggta aatttattcg aaagacaagt atcgatgagt tacctcagct ctggaacatt
     1201 cttaaaggtg atatgagtat tattggaccc agacctgctt taccgagaga ggtggaacaa
     1261 tacggtgact acgaaaggca gcggttgttc gtacagcctg gtcttagttg ctattggcag
     1321 attgctccac atcgtaatga actgtccttt gaagaatggg tggaacttga tgtgaagtac
     1381 atcaatgaaa gaagctttat cacggactgg aagattatct tcttgacttt acaggctgta
     1441 ttatttggat atggtgaatg attatatata gtattaggag ttgaaatatg tgaatattat
     1501 tttattatcg ggtggtagcg gtaggcggtt atggccgctg tccaacgata ttcgtagcaa
     1561 acaattcatc aaactgttta aggatgaaaa tggcgaatat gaatctatga ttcaaagagt
     1621 ttatcgccag attacaactg ttgacccaga tgcaaaggtc actattgcaa caagtaagtc
     1681 tcaggctagt gcaatccata atcagttggg agataaggct tcgatttgtg tagaaccttg
     1741 tcgtagagat actttccctg caattttgtt agctgcagca tatcttcacg atgaacttgg
     1801 cgtaggtgat gatgaaagtg ttgcggtttg tcctgttgat ccttatgtag ataattccta
     1861 ttacgaaggc gttgaagccc ttgagaagtt ggtgcagcag aatacggcta atttgacttt
     1921 gatgggtatt gcacctacat atcctagtga aaagtacggt tatatcatcc ctgaaagttc
     1981 taacgaagta agtactgtta aggagtttaa ggaaaaacct gacaaagcaa cggcagagaa
     2041 atatttagct cagaatgctg tttggaatgc cggtgtattt gcgttcaaac ttggttacct
     2101 gttggaaaaa gctcacgcaa tggtggattt tgaagattat cgtgatttgt attccaagta
     2161 tgagacttta accaagatca gctttgacta tgctgtggtc gaaaaagaaa cgagcattca
     2221 agtgatgcgt tataacgggg attggaaaga tgtcggaact tggaacatga tggctgaagt
     2281 tatggctgat aatattaagg ggaaagctgt aatggatgat acctgtgtta atactcagat
     2341 tgtgaatgag ttgaatattc ctattcttgc tatgggctgc aaagatatgg taattgcagc
     2401 aagtggcgat ggaattctga tttctgataa agagcgcagt ggctatatga agccgtatgt
     2461 tgagaaaatc agtacggaag taatgtttgc agaaaaatcc tggggtacat ataccgttat
     2521 ggacgttcag gaaggctcca tgactgttaa agtttctatg caggccggac agaagttgag
     2581 ctaccactac cacaattctc gcgatgaagt ttggactgtt gtagcaggac agggtaaagc
     2641 gattgtagat ggtatgaagc agattcttcg tgcgggtgat gtggtgacta ttgccgcagg
     2701 atgtaaacat acaatcgaag ccgtgacaaa tctggatatt atcgaagttc agattggtga
     2761 aactattagc gtcgctgaca agtataagag ttctttgaac taaaagttcg cgaggtagtt
     2821 attatggaga tactaaaatt tcaacccgtg ggtaaagatt atctttgggg tggagaacgg
     2881 tttaagacgg aatttggaaa aaattgactt aacaccatta agcttatacg tgggaatgct
     2941 tagttcatct agacgataac tacgtacgga aatatcaaat atgaaggaca gaatggtaaa
     3001 accgagttgt ggtatgtatt ggaagctaca ccggggggtg atttatgctt ttgagtacga
     3061 tgtaacagaa gaaatgttac gaaatgctgt gatggacaga tcacttggga aacatttata
     3121 taaagaagaa gtacacaagg gagatctttt ttatgtcagt tcatggtatt ggcgctggag
     3181 tattagtggc tgaaatacag gaaagctcaa atgttaccta tcgagtttac gattatgatc
     3241 gagtagataa gaatgataat aagtgagaat tgtactttga aaaagcgatt caggtaatga
     3301 atatggaagc cactttgaaa ttggagaaaa gaaataagct cattcatttt tatcctggtg
     3361 gctctcgtga aatactgtgt agatgtaaat actgtgagcc cgaaagaatc caagtaaaga
     3421 cggggttttc gttctcggta aaagaggaat cttttcaagt tcttttatgt atatcggggg
     3481 acggacaagt tgagtcggca aatggtgtta tgaaactcat tagatttgta aaaaagatat
     3541 tgtatgtttt tgccaacgaa tattggaaga tgttaagtgt tgagcgatgc ggacttactg
     3601 aaaattagat ggggatagag ggaggatatc taatgtcacg aatgagattt atgaacacag
     3661 aaattgacaa tcttacaatg ggtgaagtat tggtagaggt tgaaaaaatg attcaagata
     3721 ggagatgcgg atatgttgta actccgaatg tggatcacat tgttcagttg gaaacaggaa
     3781 aagagttgca agatgtttat aaaaacgcat ctttaatttt gacggatggt aaaccattga
     3841 tttggatttc aaagttttat aagacaccaa taaaagaaaa aatatcggga tccgatatat
     3901 tttctttact gtgcaagatg gctgcagaaa agggatacac aatgtatttt ttaggtgcgg
     3961 cagagggcgt tgcggctaaa gcggcagata acctgatgaa aagatttccg ggattaaatg
     4021 ttgtaggaac atactctcct ccttatgggt tcgaaaaaga cgaagatgaa atgtttaaaa
     4081 ttgattccca aattaaacga gcaaatccag atatgcttat tgtgggtttg ggttgcccta
     4141 aacaggaaaa atttatgttt tataactgtg atagattggg agtacctgtg tcttttggtc
     4201 taggagctag tttggacttt gaggcaggga atgttaaaag agcaccaaaa tggatgtcag
     4261 attatggatt tgagtggctg tttagaatta ctcaggaccc taaaagaatg gcaaaacgat
     4321 atttggttga tgatttaaaa atctttaaat tagcactaaa atatcggaaa aaataaacta
     4381 tattcaatga ggacaatgaa gtatgattat tacaaaaaca ccttttcgca tgtctttttt
     4441 tggcggcgga acggatatgg aagattattt tcgagaaaat ggcggtgcag tgctttccac
     4501 tacttttgat aagtactgtt atgtgaatgt gcgtcatttg cctagatttt ttgattacag
     4561 cacagagtta tcatattctc gtactgagag agtaacaaat attgaagata tcgagcatcc
     4621 tgcaattcgt aatgcgatga aaatgctaga tatgcatgaa attcgcctta catatgaagc
     4681 agatcttcct gcacgttctg gtctgggtac atctagttct tttgccgtag gtatgttgaa
     4741 tgctttctat gctttgaagg gcaagtacgc tgacaagaag aaactcgttg ataaagcaat
     4801 ttatcttgaa agagtccttt gtaatgaagc aggcggatgg caagatcaga ttgcagcagc
     4861 ttatggtggc tttaaccgaa tcaatttcaa ctcagatggc tatgaagtat tgcctattat
     4921 tattacgcct gagcgtaaaa agcagcttaa caacaacttg atgatgttct ttactggatt
     4981 caccagattt tcctctgatg ttcagaaggc aaataatgtc agcggcacag aggacaaaag
     5041 agtaagactg aaaaaaatgt atgagcttgt ggatgaagca gaagcggttc tgactgataa
     5101 gaacagagat ctagatgatt tcggcagact gcttgatctc acttggagat tgaaaaaggc
     5161 taccggtggt gcgattagca ctggaagtat cgatgaactt tatgaaaagg gcatggctgc
     5221 tggtgcattg ggcggtaaac ttctcggagc aggtggcggc ggtttcttgg ttttttatgt
     5281 tcagcctgaa aaacaaaatg ctgtgcgttc ggcaatgagt aatttgatgt atattccgtt
     5341 tgaatttgaa aacggtggaa cacaggttat tcattacaca ccggaaatgt atattccaaa
     5401 ataaagaaat cagcgaggat aagaatatga aagttgttat tatggcaggc ggtaagggaa
     5461 ctcgcatctc atcggttgca agcgatattc caaagcccat gattaaaatt gaaaacaaac
     5521 cggtattgga gcacgaaatc gaatgtctga gagaccaggg attcacagat attattctta
     5581 ccgttagcca tcttggcgat atcattatga actattttgg cgatggttct ggaatttcac
     5641 cagttactgg tcagcccttt gacgttaaaa ttgaatactt tttggagaaa gagccattag
     5701 gaaatgccgg tgcattgttt cgtttaaagg ataatttgac cgaagacttc ctcctgctga
     5761 atgcagatgc gatgtttgac gttgatttca accgatttgt agcatatcac cgtgaaaagg
     5821 gtggcttggt gacattgttt actcacccta acagtcatcc atatgacagc ggtctaatca
     5881 ttgcagataa ggatggctct gtagacagat ggcttgtaaa ggaagattcc cgtccaactt
     5941 attaccgtaa cagagtaaat gcgggactcc atgtgattag tcctgctgtt cttgatgtcg
     6001 agataaacac acctaagatt gacttggacc gtcagttatt aaagccgttg gcaggaaccg
     6061 gaaaaatgtt ctgctatgat agtccagaat acgtaaaaga catgggtacc cctgatcgtt
     6121 attatgctgt ctgtgaagat tttaaggctg gaagagtgaa gggcaaaaat cttcagaata
     6181 agcagaaagc tgttttcctt gatcgtgatg gaacgattaa tcaatatgtt ggctttctgc
     6241 gtcatattga tgagttcgaa ctaatcaagg gtactgaaga tgcagtgaaa aagatcaatg
     6301 ccgctggata tctggctatt gtaataacga atcagccagt aattgcaaga ggtgaagttt
     6361 cttttgaaga attggaagag atccataaca aaatggagac acttcttgga tataagggcg
     6421 cttatttgga tgcagtttat tattgcccgc atcatccaca taagggatat gaggaagagc
     6481 gcccagagct aaaattcgat tgtgaatgca gaaagccaaa gcccggaatg ttaattcagg
     6541 cagctacgga cttcaacata gatttgagtc agtcttggat gattggcgat ggcgagaatg
     6601 atattaaagc gggccaagcc gccggctgta agactgcatt ggttggcgag agtgagtatg
     6661 gacaggatat gaccatagca accctgagtg aatttgcaga taagatctgc taagggatgt
     6721 tttatcacga aaggagagta taaacaatga tagacaatag attacagaaa cacattgatt
     6781 tgctgattaa acgctatccc actcttgaag taatcaagca ggatattgtt gacggatacc
     6841 tgattatgga ggaatgctat ttaaatgatg gcaagctgct gatagcagga aatggcggat
     6901 ctgctgctga ttccgagcat attgcgggtg agctgatgaa aagctttaag acgcctcgcc
     6961 ctgttagtag ggaatttgca gataaattga ttgcggttga tcctgtaaag ggttctgaat
     7021 tagctaagaa tcttgaatat ggtttgatgg cgattcctct cgttgctcat gaagcgcttt
     7081 ccactgcgta tattaatgat gttgatggcc taggtgtttt cgcacagcag ttgtttggat
     7141 ttggaagacc tggcgatgta ttccttggta tttcgacatc cggcaatagc aaaaatgtga
     7201 taaatgcaac tgttgttgct agagctcttg gtatgaaagt aattggctta actggagcca
     7261 aaggtggaga acttgcaagg gtagcagatg tttgtgttaa agctcctgaa acagaaacat
     7321 atatgattca ggagtaccat ttgccaattt accactgttg gtgtctaatg ttggaagata
     7381 aattttttgg gaatagataa aatatctaag cccatattga aaggataaaa ttatgaatgt
     7441 actatttata acaactcaat ttccatatcc tctagataat ggcggaaaaa ttggtgcgta
     7501 caacggtatc agtgttttat ctagagacta cgatgtgacg gtattgtctt tcaccgaaga
     7561 accgcagtat gttgaagaag gtgtcagttt ttttcgagag aaatttaata gtgtaaaatt
     7621 tgaaaatcct gtgatacatg atgtacacat aagaaataat ccaatgaaat tggtagaagc
     7681 tttagcaata gggtattgtc tacgccaacc ttatatagtt tcaaagtttt atgattcaaa
     7741 aatgctgcag ttaatagatg aaaaattttc taacaatgca tattgggata tcatttttat
     7801 tgactatctg aatatgcaaa gttatggtca atacatacgg gagaagtata aaacacaatt
     7861 tggtaagtat atcttaaaag accacaacat agaatatgat ttagtcctac aggaagctga
     7921 atcaagtcga ggtttaaaaa agaagttgct tcagttagag tataagcgta ctcgaaagta
     7981 tgaagaggcc gcagtaaata gggccgatta ttgctttagt gtgtgtgatg caaatacgga
     8041 agtactgaaa aagattaatg acaacagtta tacgatgctt ccaacttatg aaatgcttac
     8101 aggggtgaaa aaaaatatag agaagaacac gatcctatat ttgggtaacc tttcttggaa
     8161 atccaatatg caaggattag tttggttttt agagtcaatt tatccaaata ttcaacaaag
     8221 aattccggat gtaaaaatca caattgtcgg tagcgggcca gttggaacag attttagtaa
     8281 atattcaggc gtggattatc gtgggtatgt aaaagatatt tcacacattt atgacgatca
     8341 agcggtgcta attgtacctt tgttagaagg tagtggtata agaataaaaa tattagaagc
     8401 gttcaataat gaaattgcag ttgtatctac attagttggg tgtgatacga ttggcgcaaa
     8461 agatggaagt gagttattta tagcagataa cgaaaaagat tttgcaacgg ctgtagttag
     8521 actgcttgaa gacatgaatt tgaatgaaaa agtaagaaag aatgcaaaat tattcttaaa
     8581 agaaaaatac tccttaacgg ctagacaaga tgagtttagt gcgataatta acagctaata
     8641 tatatataag cttatctgat gatgatttat aggaggaaat atatggagat ggctgagaga
     8701 aaggaaaaga aacttggaaa taagaatatc atattatttt agaaaactcc tgttatttta
     8761 tgtttgtgtg attgttgcgg ctatgagtta ccaccttaca ggcaattcca atgtagcgtc
     8821 attaattgct gtagtaggag tagtttatgc tattgtaaag gcgtgtaata acgagtatct
     8881 ggctatatgg gttatgtttg tattaggttc tgatatatgg gggatagtgc catatattaa
     8941 cattggtagt ggtgtgttgc attggtcaga ttttaatgtt atagtagcga tatttctctg
     9001 cttgagattc atcaaagtta aagctaggag tagatacggg attgtctata gtgtaatgct
     9061 tatatttatt ttgattgcat gtgtgcaatc gcattcaatc tatggacaag atgtacatac
     9121 aacgctctta acagctagag aattgctagt tgtaatatcc ttttggccga tagtaaaaat
     9181 agtatattca gaaagaataa ctaaaaatga attcattgat ttagtgtgta aattcacaag
     9241 atggacaatg tgtatatatt ggtttgaatg gattcttgtt aatatgggat tcaatataac
     9301 ctttttacag acaggtgcac gctggggtac tagaatctat atagatccga tatttatcat
     9361 tttatatttc tttataaatc tatatgcggt tatatataaa agagagggct ataatagtac
     9421 aaataaaagt atatggaatt gcatttttag tttactgaca tttgttataa tctcgcaagg
     9481 cagaagtggt atattatatg tagtaataac ctcggttatt atgcttttaa tttcgcttaa
     9541 accatccaag attattagat ttggtatatt aggaattgcc ttaattgcat gtctagtgtc
     9601 tgtgacccaa attagagagg tcgttatatc ttcgtttgta gagtctcaga gcagtgacac
     9661 aggtaatatc gcatatagaa ttgctgaaca agattatttt gaaaataaat tagaaggtca
     9721 tgaactattt ggagttggta taccaaacaa tcatgatgcc agagctgtag aatattcagg
     9781 caaaacaata gattttgagt ctaaaaaata taatccctat tatcttgagg acttaggcgc
     9841 ttttgaaatt cgttatagat ttggtataat tgcatatgcg ttatatatag ttgctttagc
     9901 tataagtatt atagatgccg gatttcgaag agacaaatcg gtcgccgcat ttgcgggtat
     9961 ggcattcatg gtgtttatgg cgcttaattt taatttgcta gcgtatatta cagcaagacc
    10021 gttcggattt atgatttctt taattttgat agaaatatcg gcattcaatg atgaattcat
    10081 atatattgaa taggtaaggg agagcacaga tgaatttaga tcgattaaag aaaatagcaa
    10141 atatgatttc accaatcata aaagtgataa atgcgccgtt agcgtatttt agtgggaaat
    10201 cagattatat ggagaaggga atattgtggc ctactgtaac aacctactcc aataacagta
    10261 gaaaagtgat tcaatataca actttaccta aaaacgtaga caacatggct caaatgcaat
    10321 gggctgggga aagttttgag gacatatgta tagttcttca aggtccagta ttagaagacg
    10381 gtgattttac actaaatacc gttaaaatgt ataataaata ctatcctaaa gtaaaacttg
    10441 tagtgtctac ttggatagga ttatctgaag agacagaatg caagttgcgt gaagctggtg
    10501 cagatgttgt attatgcaag aaacctgaaa aaacaggtgc gtacaatatt aatctacaac
    10561 ttacttctgc atacgaaggc gtaaaaatgg cgcaaaaact ggggtgtagt tatgcgctga
    10621 aaacgcgaac tgatcaaaga ctatatgcaa atgatgtact aaagtatttc caaacattat
    10681 tgaatatttt tccatcttcg gactgtaaag tagtgaatca gcgattaata tttacgactt
    10741 atggaaaatc atttagatat cttccgttta atttatgtga ttttttggta tacggagaaa
    10801 ctagtgaatt attaaaatta tatggtattc aaagagataa ccgagcatca gactttggga
    10861 aaattattta tactgaggaa aaagagttta ataaaaagat atttacacta ctagaaggaa
    10921 aacaaggtgt atctccatat gatgtttttc cagattttga tgcactatat aaaaaatata
    10981 tgtttgcaga atactatatt gtgtataatt atttcctgga aaatattgca agtaatgaat
    11041 cttatggtga cttaatggat gcctattata gttatttaaa gaaatatgca gttgttgctg
    11101 attcagaaaa attgttacta tattggccga aatatgttca atataatgtt aagttcgaaa
    11161 atgaacttga tttctatgcg aagttggatt caaaaaaatg gatggatatt tatgctaatt
    11221 atacaccttt tagtcagtga ttatatggaa aggatgaggt gaataatgaa aatcggaata
    11281 gtgactattg ttaatggggc aagttttggt aacagattac aaaattatgc gactcaaaaa
    11341 gtgttggaaa ataacggcta tacagttgag acaattaata attgttttga caaagaaagt
    11401 accctatcca ttgttatcaa aaacgctatt tatacattat ttaagtttat accgcatttc
    11461 aaaaagtcaa aaagtatctg gaaaagaatg tatacatttt atcaatggga caagcagtat
    11521 atagattatt ctcatcgtaa aattgatgca tctaaaaaaa acagctttgt gagtataaag
    11581 aatgattatg actatttctt ggttggtagc gatcaaattt ggaatccaaa atatgcagcc
    11641 aataagggat ttgaactttt atcatttgtt ccaaatgaaa aaagagtttc attatcaacc
    11701 agtttgggag ttccatctat accaagtgat ttagaaaaat tatatgtgac agagtggaat
    11761 aagtttaaat caatatcggt acgtgaaaat caagcggcaa aaataattga ggacttgaca
    11821 ggcacaactg cggctgttca cattgaccca actttaatgt tggagcgttc ggaatgggaa
    11881 gcctttcaaa aaaaaccaga caatattgat gaaaattaca tcttgtcgtt tttcttaact
    11941 aatccgccta aggatactgt tgatgtatta gataaggtcg ctaaagattt gaaaataggt
    12001 gttctcacct tagatgcgtc ttgggaatat tttcaagata ttactccgaa tcattttgtt
    12061 tatttgattt cacatgcaag aatgatttat acagattcat tccactgcac agttttttca
    12121 ttgctatttg gaaaaccggt tgtggtattt gaccgttttg atagtgaaaa aatgaattct
    12181 aggttaagta cattgttaaa taaagtaaat aaagcacatt tttgggtagg aaaaaaagct
    12241 gatgaagcaa tgattcgtgc aatagcatct gaacctgaat atgacataga taagctgatg
    12301 gtatcagaga gggaatgtgt aaaagcttat cttaaagagt atttacagta aaattttgaa
    12361 atgggatgaa aatatggagc atttaagtga ttgttttggt aaaatgaaga ttgttacgac
    12421 ttcatgcgca agagaatatt ttaaggatga tttggataaa tcagagtatt catgttttgt
    12481 tgattctcga gcagctatgt ttttcgcaat gggacaagct ttagctaaaa gaaggaatgt
    12541 agttgtaatg cttgctgatg aattgacatc gtgttatacg gcactaacag aatgtgcatt
    12601 tcaacaagtt gatttaattg tgtttgcttt tcagacagtc agacatgaaa ttgatttagc
    12661 atctttgaag aattgtggtt gcaatctaat taactgtagc gcccaagacg atattgatag
    12721 agttgtgtcc aacactaagg ggttaaatca gacaacagta attaaaatca atatgaattg
    12781 tgagtcggat aaagatcata tatgtgagta tatagacctg ccagacatac ctgttttttt
    12841 ccacggtagc ttatactcac ctgcaagtaa aaagaaatat gagcagtatg gacaggtatc
    12901 gaagtattta ggctatctcg tggggggaaa agatgcaata ctcataattt ctcacaatga
    12961 gtttttaaac gatatcagtg tatggattaa ttattattct tttggagaaa ttcaacccaa
    13021 aattattata tttgacagtc cgaatgacta tcaatataaa tggctgtgtg acttagaatg
    13081 cagtgtagct cattcaacca accgattttt gtctgaggat ttagccttat tgcaaaataa
    13141 cggaatttcc attgtgttaa gaggtgataa ttatgatatc gtcagttaaa aatgttcaaa
    13201 ttttagtatc tttattaaaa cagcataata ttaaagatat agttatctcg ccaggtagta
    13261 gagattttca attggtgcat agtgtagaaa cagacagttt ttttagaacc tatacggttg
    13321 tagatgagcg cagtgctgcc ttttttgcta atggactttc tatggcgatt gataagcccg
    13381 tgtgtgtatg ttgtacttct tcaactgcct cttcaaatta tatggcggca gctaaagatg
    13441 cttttgctaa tggcatccaa cttgtacttc ttactgctga tagagattac cgccgcttat
    13501 atcagatgga agatcagatg attgatcagg tagatatgta tggggcttat gtgcgttcta
    13561 acgttgatat tcctattgtt aaggattctg atgatgaatg gtatgctacc agaaaaataa
    13621 atgaagcaat attggagtta aatcatggcg ttaagggtcc cgtgcagatt aattatcaat
    13681 taatggattt aagtgcgccg tttgttgaga ccatgccaga gtatcgtaaa attacaagat
    13741 atcaagagga tgatttactg tcatcagtag agatttttaa acagaaacta ttatcaaaaa
    13801 agagaataat ggttttatgt ggacagcagg aagaaaataa tttaagtgcc ttattggaag
    13861 agttctttat taaatataat acaattataa tcaaggataa ctattctaat atacacggtg
    13921 aacactttat taagactgtt ctagtaacag aaagaatgag tgtaaatgca tttattgatt
    13981 attgcccaga tttggtaata actattggta aacatgtatg gtcttttgtg aaatatactc
    14041 ttagagataa aaagcttaaa tttgagcatt ggcggatatc tgaggatggt aagattgttg
    14101 atccgttcaa caagctaaca tctgtctttc agtgctcagc ggaatttttt atggagactg
    14161 tcactgctgg tgtgaatagc aataataatc ttgagtatta taatctatgg aagacaagat
    14221 tatcaaaagt ttgctatcca gagttagtct atagcaattt ttatgcaatt tcagaactag
    14281 taaaaaaaat tccagatagt gcattagttc attctagtat tttaaatgcg gctagattgt
    14341 atgactttgt atctgatgat aaagcattac tcagttttgc taatttaggg gctgatggta
    14401 ttgatggttg tttaccaaca tttttaggtc aggcagaagc agataaaact cgacctgcgt
    14461 atttaatttc aggagacttg agtttctttt atgctatgaa tagtttatgt ggtgacattc
    14521 cagataatgc acatattttc cttattaaca atcattctgg aagtgaattc catacaaatt
    14581 ttggtaagtt ttacaacgga tcattaagtg ttgataagca tatagctgca ggacatattt
    14641 caaacgcaaa ggattgggca ttagaaaata atataaaata cattactgcg gattctaagg
    14701 aaagtctcaa atcggggttg gatgtattta ttaatacatt tgacagtcct gttttatttg
    14761 aggtctttac agatccggat acggatacgt ctgttctgga atctttttat agtataaata
    14821 aggtaatcac accaaaaatg tattttagaa aggcgttaga tattataaaa aagcatttac
    14881 acattaagta gatgtttatt gcgaggtgaa acaaatatgg caataaaatc ttatattaaa
    14941 cggttattgc tttatatccg tcatggagat cccaaaataa atttagatgt gagttattta
    15001 tctgaaggtg caatgcttgc tggtaagact gtgataatta cgggtggtgc aaaaggactt
    15061 ggttttgcaa ttgaaaaatc atttatagct caaggcgcaa gcgttattat tacaggaaga
    15121 gacatggatt cgttgaataa tgctaaatgt cagcttggag atatgtgttc tatcagggcg
    15181 tttgatataa ctgatgctaa aatgtatcga acgttttttg aagatgcata cgagaccttt
    15241 ggacgtattg atatactgct taataatgct gggatctcgt tacacgaaga tggatttatg
    15301 agtgttactg agaaaacttg ggataagcaa atggatacaa atttgaaagg tgtttatttc
    15361 tttacacagg aatttattaa gtttgttaaa gaaaagaatc aaatgcaagc aaatattatt
    15421 atgatgtcct cagaaagagg cttgtgtggt gatagtattc cgtatggatt atcaaaggct
    15481 gcaattaact ctttagttgt aggcttgtct aagaaatata tttctgaggg aattagggtt
    15541 aatggtattg ctcctggagt aactgcaacc gctatgactg gtatttctga aaagggtaat
    15601 ttgtataatc ctgcaacgat cggttataga tattttctcc cagaagaagt tgctcaagtt
    15661 gctttgtttt tggcaagtag tctttcaaac tgtatcagtg gtgaaatcat agcctgtgac
    15721 caggggcaat atttaagtcg aaattggggg taataacttg ggaatattat taaaaaaata
    15781 taatagcctt agtgagcctg caaaagcatc tatttggttt acactttgta atgtgttgca
    15841 aaaaggaatt ctatttttga ctacacctat ttttacaaga atgctttcaa ctgcagaata
    15901 tggtaagtat acgattttta tatcgtggta ttctattata gcaattttca caacgttgaa
    15961 tttatctgca ggtgtctata ataaaggact aattaaatac gagaataaat atgcattaac
    16021 ctcgtcattt ttaggattat cgtcatttat ttccctttgt gcattgatgc tataccttcc
    16081 atttagaacg gttataaatg ggctactgga tttatcgacg gagtttgtca tattgattct
    16141 tattgaatca atatttgagc cggctttttt gctttggtca gttaaggaac gatttgatta
    16201 caagtatcaa aaactagtac tcatcacagt tcttatggct gttttaaatc caggtctcgg
    16261 tattatatta atcttatcat ttggggagta tgctatttgg agagtacgag cttatgtcgt
    16321 ggtaaatgtt gtagtgggga tgtttattgg cataggaata ctggctaagg gtaagaaatt
    16381 atttgataaa gagtactgga catatgcgct cagatttaat ttgccattaa taccacatta
    16441 tttatcaatg acattgctga atcaggttga tcgccttatg ataaatagta tggttggagc
    16501 tagcgaggct gcgatttata gtatcgctta cactatatct agtatgatga atatcataac
    16561 aacagctata aacggttctt ttattccttt tacatacaaa gcaattaaag atggcgtgta
    16621 tgaaaaaatt aaatccgttg ctaatcaact attagtgttg atttttgtct tgtgttcgtt
    16681 tactatgctg ttcgggccag aattgatttg ggttgtcgga ggagaaaaat atatggatgc
    16741 tgtttggatt attcctccag ttgcggcatc agtatttttc atgtttttat atccgctttt
    16801 tggcaatatt gaattttact atgaaaagac aaactacgta atgtttgcat cttgtgtggg
    16861 ctctatcgca aaaattattt caaactatgt tagtattccc cgttttggat atatttcggc
    16921 tggttacacg acattgattt gctatatgtt gttttccttt gcccactaca tgttttataa
    16981 aaaaattctt agagaaaatg gtataaaaga agatatgtat aatattaaaa atattaaatt
    17041 tctttctgtc ttaatgat
//
LOCUS       DACEOE010000045        16939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEOE010000045 DACEOE010000000
VERSION     DACEOE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.047"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(121..807)
                     /locus_tag="IW774_001247"
     CDS             complement(121..807)
                     /locus_tag="IW774_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1153601.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(943..1137)
                     /locus_tag="IW774_001248"
     CDS             complement(943..1137)
                     /locus_tag="IW774_001248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153602.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(1187..1627)
                     /locus_tag="IW774_001249"
     CDS             complement(1187..1627)
                     /locus_tag="IW774_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1153603.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(1631..2476)
                     /locus_tag="IW774_001250"
     CDS             complement(1631..2476)
                     /locus_tag="IW774_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1153604.1"
                     /translation="MLINEQLVKRIITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2625..4043
                     /gene="pepV"
                     /locus_tag="IW774_001251"
     CDS             2625..4043
                     /gene="pepV"
                     /locus_tag="IW774_001251"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1153605.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(4100..5047)
                     /locus_tag="IW774_001252"
     CDS             complement(4100..5047)
                     /locus_tag="IW774_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1153606.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(5270..5620)
                     /locus_tag="IW774_001253"
     CDS             complement(5270..5620)
                     /locus_tag="IW774_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1153607.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5820..6899
                     /gene="pepA"
                     /locus_tag="IW774_001254"
     CDS             5820..6899
                     /gene="pepA"
                     /locus_tag="IW774_001254"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1153608.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7043..7363
                     /locus_tag="IW774_001255"
     CDS             7043..7363
                     /locus_tag="IW774_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1153609.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            7385..7849
                     /locus_tag="IW774_001256"
     CDS             7385..7849
                     /locus_tag="IW774_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1153610.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            8054..8659
                     /locus_tag="IW774_001257"
     CDS             8054..8659
                     /locus_tag="IW774_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1153611.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(8698..9987)
                     /locus_tag="IW774_001258"
     CDS             complement(8698..9987)
                     /locus_tag="IW774_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1153612.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENIKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            10415..10894
                     /gene="rlmH"
                     /locus_tag="IW774_001259"
     CDS             10415..10894
                     /gene="rlmH"
                     /locus_tag="IW774_001259"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1153613.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            10979..11488
                     /locus_tag="IW774_001260"
     CDS             10979..11488
                     /locus_tag="IW774_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ1153614.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            11588..12238
                     /locus_tag="IW774_001261"
     CDS             11588..12238
                     /locus_tag="IW774_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153615.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            12689..13627
                     /locus_tag="IW774_001262"
     CDS             12689..13627
                     /locus_tag="IW774_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1153616.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            13640..14692
                     /locus_tag="IW774_001263"
     CDS             13640..14692
                     /locus_tag="IW774_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1153617.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            14958..15593
                     /locus_tag="IW774_001264"
     CDS             14958..15593
                     /locus_tag="IW774_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1153618.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYRLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            15784..16575
                     /gene="msrA"
                     /locus_tag="IW774_001265"
     CDS             15784..16575
                     /gene="msrA"
                     /locus_tag="IW774_001265"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1153619.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         5378 a         2955 c         3419 g         5187 t
ORIGIN      
        1 ataaacggtg tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg caaaatatga
       61 ggaactattt tgccgtttct ttctctaata ctcaggtcaa aacgctgatt ccaaaacctc
      121 ttatttgatg ggtctgtcct gaatatcttc aaaataatcg gggtaattat gaatcacgta
      181 ttcttgctcc tcgataatca tatgtaaatg catcgtgcaa agaaatccga cttcatcaaa
      241 gttatgatca atcattgatt gaagcattgt ctgatgttca tctaatactc gaccccagtc
      301 taattctgaa atcgtcaaac gtaaccatct gaaacggttc aagtgcgtac tataactttc
      361 tagccaatcc caaatttctc gttttccagc aatttcataa caagttcggt gaaaatcagt
      421 atccaaatca aaaaaagctt ttttattttg agcgattact gcctgatcgg tttgttctag
      481 tattttttct agtttagctt gatcttgctt ggtcattttc gcactgcatt cttgcaggat
      541 cggtttttcc aacttttccc gcgtataacg ggctaatgaa gcagatcgga tatcgatttt
      601 tgaaacataa gtgccggact gtggaatggt ttttacaagc tcttgattgc gcagttggat
      661 cagtgcttct cgaatcggtg tacgcccgat agctagttgt tcttctaaca ctttatcgga
      721 gattttactt ccaggtgcta actcggaata aatgatttgt ttacgtatag attcataagc
      781 ttgctgttgc aaattattga taaccattta accttccttt ctatgcagat ataatctatt
      841 ataacataaa tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct
      901 gataggcatg cctattagag cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc
      961 ttgtatcggt ttgacacgac cgccgattct tttagcaaaa gcttcggatt cttttttgtc
     1021 tttgaaaatg agcagattgt ctaagttttt ttcccgagtg cgacagccag ttttatcgat
     1081 gtaaccgtgc attactttaa cgacatacat ggggatcacc tgcttttttc ttttcattat
     1141 acgaactact cgacacaata caagaaaaat gcttagacgg tctttattaa gaatttacat
     1201 catccaaaga agcgtacgct tggaataact gatctaactc cggattgact ttgctcagct
     1261 tcactaatcg ttgcgaatca gaaagtaaaa aacagtgttt actactgcca gtagttttta
     1321 attttttgtc ttcagttccc acaatttcgt agctaaaatc caaccgtgtc cccgtatact
     1381 tttggatagt tggatggatg ctcacttttt ctccgtaatg gatcatttct ttatagctgc
     1441 aatttacttc cagaacgggg atgatgatcc cagattcttc gattttttta tagggaaacc
     1501 ctagatgtac caataaggct acccgagcct cttcaaacca gcggatataa ttgctgtgat
     1561 gaataatccc catttgatct gtttcataat aaaaaggttc tcgcacataa cctgtaaatt
     1621 tctccatgac tcatgcctcg ttttccattg attttttaca aaaataattc atccatttcg
     1681 gaagtgcttc gctgatttgt gtcggcaaaa caatatattg ttcttttgcc agctggtctc
     1741 caatgaaact atgaagataa atagctgctg caatcgtttg gtcattttga ggaaactggg
     1801 ctaaaaatcc tgtgatcatc ccagctaaag tgtcacccat gccgcccgtt gccatagcag
     1861 cagaacctaa aggattggtg tagaagtgct ggtgaccata aatttttgtc tcatgacttt
     1921 tcaaaacgag cacagcaccg atttcctgtt gcttcttttt gttttcttta tcccgttgtt
     1981 cttgtatcgg tagagaactc aaccgttgcc actccatttg atgaggtgta agtacagttt
     2041 gttcgggata gggtagcaaa agatgattag cagcaagtaa acttaaagca gagccatcaa
     2101 tcaccagcca ttgttctttt gattggtttt ctagtacttg tttgaaacgt tccaaactct
     2161 tagaagaagt acccaatccg ggaccaatca agagtacatc tgcttgcttt attacggaaa
     2221 gaaactggtc agtatccgtc caaactacaa tcattgcttc tggcagatga ctgtgaagag
     2281 cactccgatt tttttcatca gttactacag tcgtgagacc cgctccgctg tgtacggcag
     2341 ctaaagcact cataatgatt gctccgccat attgttcatt gccgccaatc aaaacgatac
     2401 gtccaaatgt tcctttatgt gaaatttttg gacgaggtgt gatgattctt ttcactagtt
     2461 gttcgtttat cagcatctaa aaacctcctt ttactcatta tagcgtatat tttttttaga
     2521 gaaaaacaga taagaaaagg aattgtcaga ttttttgaac tatttttttc tgtaatttgt
     2581 ggtatcatga acacgaagac ttaaataagt ggaggattaa ctttatgaca attgattggc
     2641 aaaaagaagt agaagcacgt aaagaagact tgcttgaaga cttgaaaaat cttttacgtg
     2701 tcaacagcga gcgcgatgac tcaaaagtaa caccagatgc accatttgga cctggacctc
     2761 gcgacgcatt gaaacatatg ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg
     2821 tcgataacta tgcagggcat attgatcttg gcgaaggaga cgaaacgcta gggatatttg
     2881 gacacatgga tgtcgttcct gcaggagatg gctgggacac agatccttat gaaccagtaa
     2941 tcaaggatgg aaagatcttt gcacgcggat caagcgatga caaaggtcca agtatggcag
     3001 cttactatgc gatgaagatc attaaagaac ttgacttgaa actatcaaaa aaagttcgct
     3061 tcgtagtcgg cagcgatgaa gaaagcggct ggggcgatat ggcttattat ttcgaacatg
     3121 aagaagaacc agattttggt ttctcaccag atgctgaatt cccaatcatc aatggtgaaa
     3181 aaggaaatgt ttctttagct ttacgcttca aaggcgacaa tgcaggagat tatgtcttga
     3241 aatcttttgt ttccggactt cgcgaaaata tggtgccagg aacagcaact gcagcacttc
     3301 aagtaccaag tgcagatgca gctatcgcga tggaagaagc attttaccaa ttcatcgaag
     3361 caaatccagt aagcggaacg attgaagcgg acaatacgta tgtaaaaatc gaattagttg
     3421 gtaaaggcgc acatggtgca agcccacaat ctggtatcaa tgcaggttct ttcttggcat
     3481 tgttcttaga taactatgaa ttcctaggat ctgctaaaca atttatccat gtagcagctg
     3541 cttatgtcca tgaagatttt tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa
     3601 tgggcaaatt gacaatgaat gctggactat ttgcttttga agaaaatggt acagaggaag
     3661 cgaacttcat caatatgaat ttccgttttc caaaaggtgt aacagtagac ggattgcaaa
     3721 gtgacatcga acaaacagtg gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa
     3781 tggaaccaca ttacgtacca atggatgatc cattggtggc tacattattg caagtctatg
     3841 aagaccacac tggagaaaaa ggctacgaac aaatcatcgg tggcggaact tacggacgtt
     3901 tgttgaaacg tggcgttgct tatggagcga tgttcccagg atatactgat acaatgcatc
     3961 aagcaaacga atttatgagt cttgatgact tgttcagagc aacagcaatc tatgcagatg
     4021 ccatctatcg tttggcgaaa taattaatta aaaagaggat caagaattgc aaattatttt
     4081 tgcagtcttg atcctttttt tactttctac tgtggtattc atgataaaca tgatatagat
     4141 tcaattcata ttctttctta gcgttcgtga cgactgtatc caaaatcaac cctgttacta
     4201 atgaaagaag tgctaaagtc atcaatccag tacttaaaat cgcagaaggg atgcgtgtaa
     4261 taaagcttgt cagcatatat tcgcgaatca caggaatccc cgtgaccaat ccaaataaaa
     4321 agaagaagat ggtccaaatc ccaaagaaaa gcaaaggctt ataatcttta aacattttaa
     4381 taatcatcat gatcactttg aatccgtcag agaatgtatt caattttgat tcgctgcctt
     4441 caggacgatc acgataatcg attgggattt caacgaactt gaatttttta tctaaagcat
     4501 ggatcgtcaa ttccgtttcg atttgaaaac cagaactcat aataggaaaa cttttgacaa
     4561 agatacgatt gaatcctcga tagccggtca tgacatcaag aatttttgtt ttatacattc
     4621 tagtaattgt atttctaaca aggttatttc caaaatcgtg gaaaggtctc ttattctcat
     4681 caaagtaggt tccatttgat aggcgatcac caatgaccat atctgcttca ccagaacgta
     4741 atttctcgag caaaccatgt acagcttctg caggataagt atcatcgcca tctaccatta
     4801 aataatagtc ggcatcgata tcaaagaaca tttggcggat cacatttcct tttccttgac
     4861 gaggttcttt ttttacgatt gctccacctt gaacggccaa ttcgtatgtt cgatcggtcg
     4921 aattattatc atagacataa atatctgctt ctggtaattc tcgtttaaag tctgcaatga
     4981 ctgttgaaat tgttgcttcc tcattgtaac aaggaatcag aacagcaatt ttttcttgtt
     5041 gttccatttt tcactctcct taactataga aaacaaaagc taaattaaaa atacctcata
     5101 atcaaaggaa atgctagttt ttcccgctaa atatcattta tttaaaaaga aaatactgga
     5161 atatatgttt ggaaagctct atctaaaaaa ccacccacta caaaagaagg acatgatacc
     5221 tctcttttga atagtgggtg cgtattcgtt ctctcacttt tttgacgtat taatctgttg
     5281 cacctttttt tcttgaaatt tctaatacgg ttcctgtatg agcatctgct aaaaattcat
     5341 aataaacagt ttcgccatct tctattcgag aaagacctcc ccgataagca ctcgaccgaa
     5401 cggcgaattt cctaataggt tccttttcaa aagagatcca agaaccttcg attgcacctt
     5461 cttttagaaa agatgacttg atattttcaa gtacttggtc tgctgacaat acttttttgc
     5521 gatgatacca aatcgttgaa gctaccccgc tcaataatcc tagaccgatt ccaactgcta
     5581 acccgccttt aaaatagctt agttcgcttt cttcatacat gtggacatac ctccttcttc
     5641 tcacttgtta ctccttgtgc ttattttacc acactctcat attttctttg aaataaataa
     5701 ccgctaccag aaaaaataag ctataataaa tacgatcggt ctgcagaaaa aatcatattt
     5761 tatccgcaga tcaatataag tctgagagga gtttagtctg aaatttagac gaatagcata
     5821 tggaagaaaa aacatttcaa cgaatcaaag agttaacaga attacaagga acaagcggtt
     5881 ttgaacaaga tgttcgcgct tatatggaaa gtcatatgga accgctggta gatgagttac
     5941 agttagatgg tttgggcggc atttttggtc tgcgccatca tgaagaagcg gatgcacccc
     6001 gtgtaatggt cgcagcacat atggatgaag tcggattcat gctgacacaa atccaagaaa
     6061 acgggctttt taaagtcgta cctctaggcg gctggaatcc ttacgttgtt tctgctcagc
     6121 gttttacact taaaacctct actggtaaga attatccatg tatttcttct tccgttcccc
     6181 cacatttatt gcgaggaaca agcggacaaa aatcagtaga agtaaccgat attttgttcg
     6241 atgctgggtt tgaatcaaga gaagaggcaa tgagttacgg tgtccttcca ggagacacaa
     6301 tcgttcctta cgctgaaacg atcaaaacag ccaacggaaa gaatattatc agtaaatcat
     6361 gggacaaccg ctatggctgt acaatggtat tagaagcttt agaagcgttg caaaatgaaa
     6421 cattaggtca tacattgatt gcaggcgcga atgtccagga agaagtaggc ttgcgtggtt
     6481 ctaaagcttc tgtcaataaa ttcaagccag atctgttctt tgcagtagac tgctctgcag
     6541 cagatgatac agtgacgaaa aatggcacat tcggacattt aggagaaggt acattgatgc
     6601 gtatccaaga tccaggtttg atcatgcttc ctcgtttgcg cgagtacttg ctggatatcg
     6661 ctgaaacaaa caatatccct tatcaatact ttgtttcaaa aggcggtaca gatgcaggtg
     6721 ctgctcatac acaaaacgaa ggaatcccaa gtacagtgat tggcgtagtg ggacgttata
     6781 tccatacgca tcaaacgatg ttcagtattc gcgattttga agctgcacga gaaatgttga
     6841 ttcaaacact gaaaggtttg gataaatcaa cagtgaatac gatcgtatac ggaaaataat
     6901 gcgatagtga aactataaaa tctggcaaaa gattgctttt tgccagattt tttttataaa
     6961 aagaggatca atgtttgtta gttcgaaaga aaggtgtata atttaattga aaaatacata
     7021 aacagaaagt agaggacaaa gaatgattat cccaaatagt ttagaagaat tagcaggcta
     7081 cgtagaaact ggaaagagtg tatttttctt tactgccggc tggtgcggag actgccgttt
     7141 tatcaaaccg caaatgcctg aaattgaaaa tgacttttct gattggcaat ttattgaagt
     7201 agaccgtgac aagtacatag atgtagccgc tgaatggaac atttttggta tcccaagttt
     7261 tgtcgtgatc caagacggaa aagaattagg tcgtctagtg aataaagatc gtaaaacaaa
     7321 acaggaaatc gaatcgttcc tagaaagtgt aacaaaaggc tgacatctgt taggaggggt
     7381 caagatgaat caagaatata agaatatttt agtaggtatc gacggtagtg aacaagcact
     7441 tgctgctttc aaaaaagcgg tagaagtggc tcgccgtaat aatgggaaag tatatgtagc
     7501 aaacgtaatc gatcagcaat tttataactt tatgggctat gcacctattg atcaaaatct
     7561 agtcgatcag caaactgaaa cagccaagga aatgatcgaa gaatgtaaag aatatgggaa
     7621 atctgttgat tacacaaata tagaaggaat tattgcatat ggttctgttc gtgaatccat
     7681 ggcagtcaaa ttgccaaaaa aatatgagat cgatctaatc atggttgggc agtcaggtct
     7741 aaatgcagtc gaacgcttta tgacaggaag tgtagccagc tacgtgatac gtcattcacc
     7801 ttgtgatgtc ttgatcgttt ctgacgaaga agaacagcca agtaattaag caagtggaac
     7861 ttgtacgacc tttttaatca attaactaaa acagtaaagg gactgtgaca aaagtcttgt
     7921 cacagtccct tgttacgaat aaatggtgtt ccagaagagg cttcttcgga aataaagcat
     7981 gaaaaaaatt tcatgcagtg aatgcaattt tttgttaaga taaatctcag caaaggataa
     8041 tggaggaaag aacatgattt ttgcttataa caaagcacac gtcggagaca ccttgatggt
     8101 tattatagcc gacgataaag gaacagaaaa cactgttgaa cgcaaatgga atgttgctcg
     8161 gatcaaagat gaacaaggtc aagttgttgc ttggaacttt ttccatatct ccgatcatct
     8221 aacaatcgaa gggaacggcc aagtaagctt aacagatgag gaattagcga aattgaatcg
     8281 attgatcaaa gaagcaggat tcgaagaaca gcttgaagct gatcatgaac cgaagatcgt
     8341 tgtcgggtac gtaaaaacat gcgtcccaca tcctgattct gaccatttat cgattactga
     8401 gacagaagta gacaatggta cagtattgca aatcgtttgc ggtgcaccca atatcgaagc
     8461 tggacaaaaa gttgtcgttg caaaaccagg agctatgatg ccagatggaa tgatgatctg
     8521 gccaggaact ttgcgagggg tagatagcta tggaatgatc tgttccgctc gtgaattgca
     8581 tttgccaaat gctccagcaa aaaaaggaat cttagtattg cctgaggatg ccgtagtcgg
     8641 agaagcattt ccaaaataaa gaggttgtga caagtctttg tcacaacctc ttttttatta
     8701 attttcacta ttttgatttt gttttaatgc cgattgatcg atcgtcaatt caacagtagt
     8761 tgttttttct ttcgagcctt catagtaagt gattttgaca gaatctccca cttttttctt
     8821 atacaaagca gattgaagtt ctacaccaga agaaacttct ttgtcatcga tcttagtaat
     8881 gacgtcgtat tgtttcaatc cagctttttc agccggtgta gctgtttgga catttgtcac
     8941 gatgacaccg ttagttacag atgaaggaat cttcaagatt tgttcttgtt gctgagtaga
     9001 aacggcagat aagtcgacca ttgtgatacc tagagctgga cgtgtcactt ttccgtcttt
     9061 ttccagttga ttgatgatat tcactacatc gttacttgga atagcaaatc ccattccttc
     9121 tacgcttaca ttagaagtag attcagaagt acttgcgatc ttacttgagt tgataccgat
     9181 aacttgtcct tcgatattaa ctaaaggacc accagaattt ccagggttga ttgctgcatc
     9241 tgtttggatc gcattgatat tgactgtttc gttcgattca ttcgtacttg tcacttgtcg
     9301 gttcaaggaa gaaatgattc ctgaagtgac agaatttgca tattcagatc ctagtgggga
     9361 accaatcgca atcgcaggct caccaacctt cagagcactt gagtcaccaa aagaggcaac
     9421 tgtttcgact ttgtctgaat tgatcttcaa caccgctaaa tctgaataag catctgttcc
     9481 aactaattca gcttttacct tcgtaccgtc tttcatcaag acttcaagac cttgctggcc
     9541 atctactacg tgattattcg tgactacata agcagtattc ccgtcttttt tgtagatcac
     9601 gccgcttcct tcactataag cttccaaatt actatcatct gagctgctac tttcttcttg
     9661 ctgcccgaat agtccgccaa aaccgcttga ttggttttga ctttgtaagt tgataacaga
     9721 aacgacagcg tcttgaactt tatctaccgc tttggtgata tcagaatcta cattgacttt
     9781 gatattttcc acaactgttt caccggcaga attctgattg ccgcttgtag cagtagaact
     9841 attgttgcca gtgcccatcg ctgcataaaa aatgccagct gtcagtagtc ctccaacgat
     9901 ccctccaaca agccccagac ctaacttacg ccagaggcta ttatttttac tttttttcat
     9961 ctttggtgtc acattttttc tatccatgtg ttcctcctcc aattacattc atttatctac
    10021 tgttagtata gtcttatttg ttgactttag tctactttga gactgtgaac tttttatgaa
    10081 aaactttagg aaaaatcatt gttttcagat gaaaatattt gattttcttt ttccacagat
    10141 actgtgtaaa actacgcgga taggattgga aaaataaggg ataaaacata tccacagcct
    10201 gtgtataaag tggataaatt cttataaaac gtatgtttgc ctaatgaatg tgatgaaata
    10261 aggtaaaata acactgtttt ctgtggataa acctgtggat gttgtgcata actgttgggg
    10321 aaatcttttt aacaaaaata gccaaaataa acatatagac gaaaaaattg attataataa
    10381 acaaagtgag catgaagacc aaaggagaga aaacatgaat atcaaaatta tctcagtagg
    10441 aaaattaaaa gaaaaatacc tgatccaagg tatcaatgag tatgtcaaac ggctgaatgc
    10501 ctatgcaaaa atcgaattga tcgaagttcc tgacgaaaaa gccccggaga atttaagcga
    10561 agcgcaaatg agacaagtaa aagaaaaaga aggggaacgg atcttagcga aaatcaaaga
    10621 acaagaatac gtctatgcct tagcaattga agggaaaaat cctacaagtg aatcttttgc
    10681 taaacaaatc gaccaattag gtatccaagg aaaaagccac cttgtttttg ttatcggcgg
    10741 ctctttagga ctcagcgaag ccgtcatgaa aagaagcaat gctcagatct cttttggaaa
    10801 aatgacgtat ccacatcaat tgatgcgtct gatccttgtc gagcaaatct accgtgcctt
    10861 ccggatcaat gcgggggcgc cttatcataa gtgatgcggt ttttatcatt gatcctaaaa
    10921 atcttagcaa gacaaaaagt acgtaagaaa gtaaagacta aatacttcgg aggaacaaat
    10981 gaataacaat gataggttat tgcgattgcg ttatgcgatt gatttgaaag atacggattt
    11041 gattaaagct tttgagctag gcggtgtacc actgactaaa gaagaagctc aggcggtact
    11101 gaccaaagta caggataaga acaaagataa tgcagaaaat aatgtgtatg aaaaaaccat
    11161 caacaaccaa gtttttgacg cattcctaaa tggcctgatt ctcttggcgc gtggtccgca
    11221 aaaagataag ccgatagtag acacgccgca aaaaagcaag gaaatcaaat atatcaacaa
    11281 tgtgttgcta aaaaaattaa aaatcgcctt gtctatgacc actgatgata ttttagacgt
    11341 ttttgctgag gcagaaatct atccatcaaa aggcgaaatc ggggcatttt tgagaaaaga
    11401 aggccaacga aattttaagc cttgcggaga taaatatatg cgtaattttc tcaaggggtt
    11461 aggaatttat aatcggagaa aagtataaag aaatttcatg agaaatcaaa acaatggttg
    11521 ttttgatgag agctagtttt tgtatgagat taaaatatta tcttaatcga ggagctggat
    11581 tggttttatg aatgatgaat ataagaatga tgaagataag atgctttttg aagagataga
    11641 gaatcgatgt aggctcaatt ttgagcttcg gggaaagatg tctttaatac aacaaaaaaa
    11701 gtatttagcg aataaatcag agtttactct gggacatgtt gaaaaattaa tctcggattg
    11761 gattagtagt agaagcgaat ttacaaaaat aaaacagccg attaaatttg acatgaaaaa
    11821 acttttgtta aataagagtg agataggtaa tagagatcaa tatattagag ctaaagggca
    11881 agaaataatt gattcattag gtgaaatgag gtcatacaat tatttgtatg taacacatag
    11941 agctgatgga atggtcatta ctgtggggaa atcttcttct aatgatattt ttttagatgg
    12001 agatcttttt tatcaactca atacaaatca cttgtctggc actgaaaata taattttaag
    12061 gacggaatat ggcaatgaaa tatttgcaaa atatgatgag attttaaaaa attatttgga
    12121 ctgggcttgg ataattccgg ttgagtcagg agatgctaag aaactggaga gattattggg
    12181 cgatgaacta attaataaaa aagtaccaat tttaaattat tattcccata gacaataaaa
    12241 ggctattttt catgataata ctagttataa ctgtaaaaat ggaagaaaat ctgggaaaga
    12301 agtttttaaa taagtgtatt gttcgatttt aataaataat aagcaatacc atccgtctgt
    12361 gttgcgcaac atcgtactac aatgaatatt ttttcacaaa aagaaaatca tccatcgaat
    12421 gaatggaagg ttttagtata cacgaggtta atgccccctc ttttataatc agaggagtga
    12481 ggttttttct atgaatgttt ctattttctt attgctagtc tagtttctct tacgatgaaa
    12541 ttgggattct attccagttt ttattgattt gctgataaaa taacttgaat ggtggatata
    12601 aacatgataa ataagagaag aataacaaaa caaggttgtg aaatgattct caaatgaata
    12661 taatagaaag aaaaaggagg aagaatcaat gaaaatagca attgcaggcg ctggcgcaat
    12721 gggaagtcgc tttgggttaa tgcttcacca aagtgggaac gaagtattat taatcgatgg
    12781 ctgggcagaa catgtccaac aaatcaaaga gcatggatta caagcaaatt ttaatggtaa
    12841 agaggtagaa gcaaaactac caatcgttct tcaatccgaa gtagaaaaag aagatcaagt
    12901 tgatctgatt attctattta ccaaagcgat gcagctggaa aaaatgctgc aggatatcca
    12961 atcattaatc aaaaaagata cagaggtctt atgtctatta aatggtatcg ggcatgaaga
    13021 tattattgag aaatttgtac caatggaaaa tatctatatt ggaaatacca tgtggacggc
    13081 aggtcttgaa ggtcctggtc aggtcaaatt atttggaagt ggttcagtag aattacaaaa
    13141 tttaggtgat ggaaaagaag cagctgcaaa aaaattagca gacaaattgt ctgaatcagg
    13201 attaaatgct catttttctg acaatattca ctattctatt tatcgcaaag catgtgtgaa
    13261 cggaacaatg aacggactat gcacaatctt agacgttaat atggctgagc taggaaaaac
    13321 atcgactgct cataaaatgg tggcgacgat tgtcaatgag tttgccaaag tagcagcagt
    13381 agagaagatt gaactagatg tcccagaagt cattgcacat tgtgaatctt gttttgaccc
    13441 ggaaacaatt gggttacatt atccttcaat gtatcaagac ttgattaaga accatcgatt
    13501 aacagaaatc gattatatca atggcgcaat ttctagaaaa gggaagaaat atggtgttgc
    13561 gacgccttat tgtgatttct tgactgagct tgtccatgca aaagaagata gtttgaacgt
    13621 aaaataaagg agagattgaa tggaaacttc acaaaaaata tcaccgaaaa tttttctgaa
    13681 taaagtcctc gctggtacag caacaggaat cattattgga ttgattccta atgcagtatt
    13741 aggtgccatt ttaaaatatt ttagtgcgta tccctttgcg gtaacgatcg cacagttagc
    13801 agttattttt caactagcaa cacctttgat cattggtggt ttgattgcct tacaatttgg
    13861 atttgatccg atgagaatga tggttgtagc cggagcttct tttgttggtt ctggtgtagt
    13921 caaattcaca cctgatttgg gtgaaacggg tgtttacgtt ggtgctggta caggcgatat
    13981 tattaatacg atgctgacag catcgattgc tgtcgggctg attttactga ttggaaatag
    14041 ttttgggtca gtgtcgattg tgttaacacc gattgttgta ggtgtaggtg caggtttact
    14101 tggtttttat ttattccctt ttgtaacagc aattacttct gcaattggat cattgattaa
    14161 taacttcact accttacaac cgctgttaat gtctatcttg attggttgtt cctttgcctt
    14221 tttgattatc tctcctattt caacagtagc aatcggaatg gcaatccaac tagatggtgt
    14281 ttctgctggt gcggctgcga tgggcgtagc tgcaaccact tttgttttag tagtgaactc
    14341 ttggaaggtt aataagtcag gtgtgacaat tgcgattgct ttaggcgcta tgaagatgat
    14401 gatgcctaat ctatttagaa aacctgtgat tcttattcct tgtttagtta gtgcagtgat
    14461 tacagctatt cctgtcgctc tattttctat ttcaggtacg cctgcttctg ctggtttcgg
    14521 cgtggtcgga cttgttggtc cgttggcttc tttagatgca gggctaaatg gctttcttgt
    14581 tgttctttgt tggttagtca tccctgtttt tgcggcacta attactcaat ttttatgcga
    14641 gaaagtattt aaactgtatg atcgagaaga agtcttcaaa tttttaggct agtagatttt
    14701 actaagaaat tttcaatcaa tggatgcgta agtgagatgt caacaacatc tgacttacgt
    14761 ttttttgcct ttttgagtag gagattgatg ctgacaactg aactcttcag tgccattcga
    14821 ttttttaaaa tgtagaagaa actcaccttt atgttattga ttgttcaaaa ggcaactaga
    14881 ataaaaatat gtgttaaaat gagaaaaaat ttgattgttg gatgtatcga ataatcacta
    14941 aaaagtagga gaagaaaatg atttttttgc aaaaatatgg tttttatttt ttattattag
    15001 gtgtgattag tgatttatcg acaccgtata tccttggcct tttttatccc aaactgaatc
    15061 aaatgactac cgtaattagt gtgtttggtg acgtagatag tccagtcaga cgtgctttct
    15121 tggtctggtc agttgtatct ggactttttt ttgttctttc gttgcctgcg ttgtatcacc
    15181 tatttattgg aacatcaaaa acacttgcaa tacttgctgt tgcaacagtt ggtttatatg
    15241 ggataggtga ttgtattttt actgggttat tcagtatcaa tacgaatgaa agctcttgga
    15301 atctatcgac ttggatccat aatataggtt ctgggctagg ctatgcaggc tttctacttt
    15361 tcccattgtt acttgttctg ctttatcgtc aaagtggtca aggtacgctt agccattttt
    15421 atcttgtttt aacagttatt agtttactaa ttgcaggtct ttacagatta gcacggattc
    15481 cgtcgattag tcagtttgcc ttctttaagc aattgggctt ctttcaacgc ttaagttttt
    15541 tctttaacta tttgccgatg atttgtttaa gtttttttca actacgaaag tagaaaaagc
    15601 agtgtgaagc tgattaagta aaagaatttc tctatgggta accattcgtg tggatgtgtt
    15661 cgactaagaa tagagatgag gagagcgaaa agcaagttag tcttaacttg ttatgcgttg
    15721 tgctaaattt caggtagtca ttttttgaat gaataagaag aaaacttgaa agagggtgac
    15781 ttcatgttag atgtggagca gatggaaata gacctttata atattatttt aaatcgaaat
    15841 acaagagaat gggaaagaac acaatttgaa gaggcaaaag aagacctagc aaaaatgtcg
    15901 ataaagcagg tgatagaaaa actagaggtc aacttgcgtc cgctagcact acgagataat
    15961 ttgacaccag atgcgatgga tttttattta agaatcacag gggagtcagc gagtgcaaag
    16021 gcttatgatt atgccaaaca taagataact tcacccttat accaagaacg ggcgattttt
    16081 gccggtggct gtttctggtg tatggttgag ccttttgaaa caaaaccagg tgtaatctca
    16141 gtgttatctg gttatacagg tggagagacc aaaaaaccaa attacgatca agtcagtggt
    16201 ggatatactg ggcatgttga agctgttgag atcatatttg atacacgtca aataacctat
    16261 gaagagctgt tggagattta ttggcaaatt tcagacccta cagatgcctt tggtcaattt
    16321 caagatcggg ggaaacaata tcgttcaatt ctatttgttg ttaatgaaaa gcaaaggcaa
    16381 ttggctgaaa aatcaagaga acaactgatt caatctaacc gatttagccg tcctatcgtt
    16441 acagagattc gccaagcaaa gactttttgg ccagctgaaa atcatcatca acaattttat
    16501 cagaagcaac ctaaaagata taaaaagatc aagcattcgc atcagcttca tttatttttt
    16561 cagcgacata gatagaggaa ggttgtggcg caaagcgttc agctccgaca aaataatgag
    16621 caactgacaa aaatagcttt tcatattttt ggacagttat gaattatttc gaggagttgc
    16681 tttgagaaca ccgaggaaaa ggttgtggcg caaagcattc agcttcaagt cgcaaggaaa
    16741 caggaggtat gacaagtctt ttgtcacaac tcctgttttt ttactagcct ttatttccat
    16801 gtgaaagaga attcgaattg tagaatcttg atttttctat ggaattttgt agagaatcat
    16861 cttgataatt aatagacacc taatcgaatt ccacattaat gatgaactaa attccgtata
    16921 aaagcgcaaa caataacta
//
LOCUS       DACEOE010000046        16725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEOE010000046 DACEOE010000000
VERSION     DACEOE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.048"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            175..1281
                     /locus_tag="IW774_001266"
     CDS             175..1281
                     /locus_tag="IW774_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1153620.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            1298..2326
                     /locus_tag="IW774_001267"
     CDS             1298..2326
                     /locus_tag="IW774_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ1153621.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            2332..2811
                     /locus_tag="IW774_001268"
     CDS             2332..2811
                     /locus_tag="IW774_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1153622.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2827..4122
                     /locus_tag="IW774_001269"
     CDS             2827..4122
                     /locus_tag="IW774_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ1153623.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            4133..4957
                     /locus_tag="IW774_001270"
     CDS             4133..4957
                     /locus_tag="IW774_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1153624.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            5122..6021
                     /locus_tag="IW774_001271"
     CDS             5122..6021
                     /locus_tag="IW774_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1153625.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(6147..7016)
                     /locus_tag="IW774_001272"
     CDS             complement(6147..7016)
                     /locus_tag="IW774_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1153626.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            7169..8629
                     /gene="rhaB"
                     /locus_tag="IW774_001273"
     CDS             7169..8629
                     /gene="rhaB"
                     /locus_tag="IW774_001273"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ1153627.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            8626..9882
                     /gene="rhaA"
                     /locus_tag="IW774_001274"
     CDS             8626..9882
                     /gene="rhaA"
                     /locus_tag="IW774_001274"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ1153628.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            9896..10741
                     /gene="rhaD"
                     /locus_tag="IW774_001275"
     CDS             9896..10741
                     /gene="rhaD"
                     /locus_tag="IW774_001275"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ1153629.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            10743..11054
                     /gene="rhaM"
                     /locus_tag="IW774_001276"
     CDS             10743..11054
                     /gene="rhaM"
                     /locus_tag="IW774_001276"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ1153630.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            11136..12287
                     /gene="fucO"
                     /locus_tag="IW774_001277"
     CDS             11136..12287
                     /gene="fucO"
                     /locus_tag="IW774_001277"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ1153631.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            12414..13172
                     /locus_tag="IW774_001278"
     CDS             12414..13172
                     /locus_tag="IW774_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ1153632.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            13272..13916
                     /locus_tag="IW774_001279"
     CDS             13272..13916
                     /locus_tag="IW774_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1153633.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            13923..14930
                     /locus_tag="IW774_001280"
     CDS             13923..14930
                     /locus_tag="IW774_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ1153634.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            14946..15788
                     /gene="kduI"
                     /locus_tag="IW774_001281"
     CDS             14946..15788
                     /gene="kduI"
                     /locus_tag="IW774_001281"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ1153635.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            15808..16611
                     /locus_tag="IW774_001282"
     CDS             15808..16611
                     /locus_tag="IW774_001282"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1153636.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT         5391 a         2869 c         3803 g         4662 t
ORIGIN      
        1 acgttgtttc tccaagttct ttttttaatt tccaagtaac tgaaagttac ttttgttatt
       61 taacatttag gtcaatttta gacagttttt gttcaaaaag cgatatttat agtggacggt
      121 attactgcta tattaaggat ataaaatgaa agcgtttaaa aaagtggcgt aaagatgatt
      181 attaaagaag agatttttgt acctaaagaa ttagtgaaag aaaaaattga cctgctcgtt
      241 gaaaacctga caacgatcaa agacgataat ggtgaatttt tgttggactt tgatggactg
      301 aaagtagatg acaaaagctg gacagtgtgg aattggccac aaggtgtcgg tctatatggt
      361 atttataaaa attaccagat gacaaaaaat cctcgcgctt atcaagtggt caatgaatgg
      421 tttgaggatc gtatggaaga aggcgctccg cccaaaaacg tcaatacaat ggcaccgcta
      481 ttgactatgg cttacttgta tgaagacacg aaagattcac gctatttgcc ttatttagaa
      541 caatgggcag aatgggtcat gaatgacatg cctcggacaa atgaagacgg gttacagcat
      601 gccacatatg gaccggaaaa taagaatcag ttgtgggacg atacattgat gatgacagtg
      661 cttcctttgg caaaaatcgg gaaactgttg aatcgcccag aatatttaga agaagctcgt
      721 taccagttta tgatccatat caaatacttg atggataaac gaacaggatt gtggtatcac
      781 ggctggacgt ttgaaggaaa tcataactat gccgaagcat tttgggctcg tggaaactgc
      841 tggttgacga ttgctattcc agagatcatt gaaatcttag aattatcgaa agaagatgca
      901 ttgcgcaaat tattgatcga aacacttgaa gcacaagtgc gtgcattaaa aacttatcag
      961 tctgaaagcg gtttatggca tacgttgttg gatgatcctt cttcttacgt agaatcttct
     1021 gcaactgcag gatttgctta cggtattttg aaagcggtcc ataaacgtta tttaccacaa
     1081 gaatataaag aagtcgctta tcgtgctatc caaggattgc ttgaacaaat cgatgaaaaa
     1141 ggagaagttc aaaacgtttc aatcgggaca ggaatggggg acagtttaga tttttatcgg
     1201 aatatcggaa tcacagcaat gccttatgga cagtcgttaa cggtgctttg tttgacagaa
     1261 ttgctcgtct cttattgtta aaagaaagga aacccatatg aaaaaaacat cactgctgtt
     1321 tgcagcaagt tcgcttagcc taggtctgct aggtctaagc ggctgcggaa ataattctag
     1381 tgtggcaact gtcgcagatg cagatgtaaa tgagatagtt accttgcgct ttgcttatgc
     1441 aagcaacagc cagccagtaa ttgatgcaat gaatgaattt gggcgtttag taaaagaaaa
     1501 aacagatggt tccgtacaga tccagtattt cccagatgga caactgggtg gagaacgtga
     1561 actgatcgaa ttgacacagt caggagcagt agatttcaca aaagtaagtg cctcagcgtt
     1621 ggaaagtttc tctaaagatt attcgatttt ttcagttcct tatctattca atgatgaaga
     1681 acatttcttt aaggtgatgg ataatgaaga aataatggag ccaatctacc aatcgaccaa
     1741 aaatttagga tttaccggat tgacctatta tgattctggt cagcgcagct tttatatggt
     1801 agacggtccg atccatacgc cggaagattt gaagggaaag aagatccgtg tgatgcaaag
     1861 cgaaaccgct atcaaaatgg tagagctttt aggcggttcg cccgtaccta tgggaagtga
     1921 tgaagtgtac acgtctcttc aatccaattt gatcaacgga tcagaaaaca atgaatttgt
     1981 cttgtataca gctggacatg gtggtgtggc taaatactat tcctatgatg agcatacacg
     2041 tgtgccagat atcatcatca tgaatgatgc catcaaagaa cagctaactg atgagcagga
     2101 aaaagccatt gaagaagcag cgaaagaatc aacggttttt gaaattgaag cttttgcaaa
     2161 agcaattgaa gaagaaaaag ctttagcaca agaagaacat ggagtccaat tcaatgatgt
     2221 agataataca ccgtttcgag aggcagttgc cccacttcat gatatattca aaaacgatcc
     2281 tacctacaaa gacctttatc aaaagatcca agagcaagga gcgtagctgt gatgaaaatg
     2341 attttaaaca aggtattaga gatcctggga gctgttttgc ttgttgggat ggtcgctgtt
     2401 gttttatggc aagtattttc cagaacggtt ttaggaaatc caaatacggt cacggaagag
     2461 cttgttcgat tcggtcttgt ctggtttgcc atgttgtctt ccgcgtatgt tgtgggacaa
     2521 aaagcacatt tagccgtaac ggttttaagt gaaaaattaa ctggcaaaaa aagtaaaatt
     2581 ttagaagttg ttgttcaaag tttatttctg gtatttgctg cgatgatcat ggtgtatggc
     2641 ggatggaatg ctgtcacatt gacaatgggg cagatctcgc cttctttaag tattcctatg
     2701 ggatatgttt atctgtcagt acctgtatcg ggggtactca tcattatcta tagcctgatc
     2761 aatctaatcg atacattaag caacaagcca gcaattcaag aaaaaatata aaaatggagg
     2821 aataaaatgg caatagaagc cggattagtg ttattttttg tatttgttgt gttgcttgta
     2881 ttagggatgc cgattgctat aagtattgca ttctcatcga tgatgacttt attgttagtt
     2941 ataccgtttg atgtttctgc ttttagttca gctcaaaaaa tggtgggaag tatcaatagt
     3001 ttttcgctga tcgctattcc gtttttcgtt ctctcgggca ttatcatgaa taatggaggg
     3061 atagctaaga aactagtgga ttttgcgatg ttgttcgtcg ggcgcgtgcc gggggctttg
     3121 gctcatacga atgtgttagg gaatgcttta tttggttcca tctctagctc ggccattgcc
     3181 gcttctaccg cgattggcgg agtgttgatc cctcagcaag tggaagaagg atatgaccgg
     3241 aagtttgcga cagcggtcaa tatcgcgtca gctccgacag gtatggtcat tcctccaagt
     3301 actgctttta tcatgtattc acttgttgct ggtggtgctt cgatctctgc tttgtttatg
     3361 ggaggttatc tcgtaggcgc tttgtgggcg ctaggaatca tgctagtagc attcatcgtt
     3421 gctaaaaaga ataaataccc tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata
     3481 ataaaagagg cgattcctag ccttctattg attgtcatca ttatcggtgg tatcttgacg
     3541 gggatattta cagcagtaga agcttcagcg atagctgtca tttactccct gttgattgct
     3601 atgttttact ataaaacggt aaaactaaaa gatataccta atatgttgtt tcaatcagta
     3661 gcaatgtcag ggacgatcat gttcttattg gctacttctt ccatgatgtc tttcgccatg
     3721 gcgtttaccg gtattcctca ggcaattagt tcgttgatta tgggcgtaac ggataataaa
     3781 tttattattc tgttattagt caatcttgtg ttattactgg tgggtatgtt tatggatgtg
     3841 gcgcctgcta tcttgatttt tacaccaata tttttaccaa tcgtcacaag tgtaggggtt
     3901 gatcctgtac attacgggtt gttttcaatc atgaatctgt gtgtaggctc catcacgccg
     3961 ccagtaggta caggactata tgtcggtgca agtgtaggag gcgtaaaagc agaggcaatg
     4021 ctgaaaccgc ttcttccttt ttatggggtt atcttagtcg tcttgttttt gattacttac
     4081 ttcccgcaaa tcgtcatgtg gctacccaat atgctgagtt aggagaatag ttatgttgaa
     4141 cattacaatt agaggacatg atctttccga agtccaaacg atagaagatc ttgcagaaaa
     4201 aacaaaagaa caagggattc atacactgca gctcgcctta ggtctttcgt ttcctgaaat
     4261 accttctggc gcaaatgaga tcaattctgg gatgggaaat tatgtcaaac gagttttaga
     4321 aaagcaagaa gtgaccgtag gaattttaag ttgctatatc aatatgattc atccagattt
     4381 aacgatacga caagagttgc tgacaaagtt cgagcagtat cttcgctatg cttcttcctt
     4441 tggtgcgtcg atggttgctt cggaaactgg atgtgttttg ccggaaatcc aatatacgga
     4501 agaaaatttc acagatgaag catttgctga agctgtgtcc gttatccgtc gcttggtaaa
     4561 agctggagaa aaataccaaa tgatggtggg gatagaacca ggtttgaatc atccggtgta
     4621 ttctttagca agggtagagc agctgatcca agcagtagat tcggactatc taggaatcat
     4681 tttagatccg accaatttga tcacaagtag tgattatcaa gaacaagtcc aattggtaga
     4741 agaagctttt gaacgttttg gtgaaaagat atgtgcggtt catttgaaag attttcgtgt
     4801 cgaacaggaa aaaatcgtgc cggtaaattt gggagacggt gtgatcgagt acacaaaaat
     4861 caaagaaatc atcatgaaga atcgtccgta cctgtatgtg gttcttgaag aaacaaaaga
     4921 tgatgggatt cgttatggaa gaagtcttct tgaataatgt aaagaagacg ttggttaact
     4981 aatatttaga atggaggggg cggggctaga agatgccacg tctcttcttt tttataagag
     5041 tatataaaag cagctctttt tgattacaaa aaacgtggta taataaaatt gtgcataagt
     5101 gaacaaaagg aggtgcggca catgctccaa aagaaaatca gcggtgaaca agtcaatacg
     5161 atggtttctg atcagtatga aatctatcat gtcaaagatg tcgtcagcca aaataaaacg
     5221 atctatcatc atcatgattt ctatgagatc catgctacat taaagggaga agcgactttt
     5281 tatttgaacg gacgacaatt tacgatcaaa gcaggaagtg tgctgctcat ccattccaat
     5341 gatttgcatc gtatcgtccg ccaatcttca gatgtattcg agcgtgtcta tatctttgtc
     5401 accccttctt ttctagaaag tcgttccacg ccatggtcta atctatcggc ttgctttcaa
     5461 ccaatgggag agaagaggag tcgtgtattg caaatagaac aggaagtatt gaagcaaaaa
     5521 ttatcattta tcgaccatcc tcctgataaa aaaatttacg gtgaagacat aaaatatgaa
     5581 caagctctgg tagaatactt gatttttctc aatcaagttg tacaaaaaga agagagtgcg
     5641 caagaacaga atgctgtagt tcctaatgag cgtgtggaac aaatgttaca atatatctca
     5701 aaaaatttat ctcatccgct ttctttagag gaaatggaaa agcagttttt tgtcagcaaa
     5761 taccatatca cacgagaatt taaaaagtac actggattta cgtttcatca gtatgtaatg
     5821 aagaaaaagc tgatctatgc caagcacttg ttgcgagaat accgcagctc tagtgctgta
     5881 tatagtaaaa gtggtttttc ttcttatccg cattttctga aagcattcaa gagagagttc
     5941 ggcataacgc ctaaagaatt tttgaaacgt gatacacaga aacagttggt tcattatgaa
     6001 cattttgaag atgaacaatg agaatgatca ctaaaaaata accgcagtag ctgctgttgt
     6061 ttacatagaa gcagaaactg cggttatttt atttttatct atatatttca aaatattttg
     6121 aaaaaatgac gtacatatta tatgaatcag ctgaatttac gaaagtctct cggcgatagt
     6181 cctgtttttg ctttgaacag gcggctaaag taagcaaaat tctcaatccc tatttcttcg
     6241 gaaatcattc gaaccgtaaa ttctgtatac cgaagaaggt ctttagcgta agaaagtttt
     6301 ttgctgatca aataatcgat tggtggagaa ccgataaatt tggaaaaatc tttgctcagc
     6361 tgatatttat tcaataagaa ttgttgttct aattgatcaa gtgtaatcgt ttctttgaaa
     6421 tgttcgtcta agtattgctt cagtttgtaa acgtaggcgg gaatatcctc ttcttcgaaa
     6481 tctagctgat acttttgcaa aatcagttca ttcagcaact cgtggatagc tacagaggac
     6541 tggaaatcgg tacgggcatt cgtgccttgt tgcatgtgga tcaattctgt gatcaaatgg
     6601 tggatcttgt tttctgatgg atcccctgtt gaatggaagg tgttatggcc gtcttttgtg
     6661 aactcctgat aaaatgattg gacgttctga ccattcaaat ggatccaatc catttcccat
     6721 ggttcttcgc tgatcgtttg ataacgctga tattcttggc agtcaataaa aaataagtct
     6781 ccagctttta gctgttgctg attatcatta tatgtaagcg aacctttgcc agacaaagta
     6841 aactttatca agtaagaagg taaatgtgct cgttcagtat agtaagggat tggtgctttg
     6901 aagtaaccga tttcttggat ataaaaaaat aaattcctgc ttctttgtgt aggcgtcaag
     6961 atcactcttt ttgagtcttc cgaccatgag tggttggctt tttcccagta atccatgtaa
     7021 acactcccaa cttgtcaaga taatgcaatt atataccaaa atagtgtgaa gcaaaatata
     7081 agattctgta ttatgatagc attgttaata aatgataacg ctttattaat aaagtgctaa
     7141 tcaacaataa agtaaaagag gtggagaaat gaatccaaca tttgttgcca tcgatatcgg
     7201 tgcatcaagc ggacgactaa tgaaaagtac gagggatgca aaaggttctt tgatcttgga
     7261 agaagtacac cggttcaaaa acggctttcg gaacaaagac ggtcacgatc gctggaagat
     7321 cgattatctt atcaaagaga ttttgattgg atttgaaaaa ttaaagcaga cagggattga
     7381 tcgccttttt gcggggatcg atacatgggc agtcgattac tgtctgattg atgaacaagg
     7441 ggatcggata aatgatccga ttgcttatcg ggacaagcgt accaatggag cagtagaaca
     7501 gttttctgaa atctattctc tagataagtt gtatgagcag acagggattc agatccagcc
     7561 tttcaatacc ttgttccaat tgtttgctga agaaaaagaa aatttggcaa aagcacataa
     7621 attgttactg atacctgatt atttagggta tgtctttact ggtaacatgg tgacggagaa
     7681 aactaatgca tctactatgc agctgctgaa tgcagggaca aaagaatggg aaatagaact
     7741 tcttgaaaaa atcggtgtct cgaaggaatt atttccacca ttgacagagc cgggaacgat
     7801 actaggagat ctgcaaaaag aacaattttc aaaatatgat ttgccagatg tgacctttat
     7861 cacagtagca agtcatgata cggcgtcagc tgtattagga acgccaggtc aaggagaacg
     7921 ttggggttat atcagcagtg ggacatggtc gcttttaggg attgaaacaa agatcgccaa
     7981 tgtttctcgc aatgcttttt ctgaaaatta tacgaatgaa tggggtgccc ataacacggt
     8041 gcgttttttg aaaaacatca tggggatgtg gttgatccag gaagttgccc gaatgcaaga
     8101 gtacgagtac agttatgcgg aattggcaga gcttgctgca aaagaaaaac cgctgcagca
     8161 agtgatggaa atcaatgacc ctcgattttt gaatccccca aatatgatag aagagattca
     8221 gcaatactgt tttgaaacag agcagccagt ccctgtctca tcgggagcta tcgctcgttg
     8281 tgtgtatgat aatcttgctc tttgttacgc acaagagctg atcaaagtag aacacttaac
     8341 tggaacgaaa ttggaaaagc tgcacatcgt agggggcgga tcgaataatc gtctactgaa
     8401 tcaattgaca gcagattatg ccggaatcat tgtagaagca ggacctagtg aagccacagc
     8461 aatcgggaat gttgccatgc aaatgctcag tacaggtcag ttttcgaata tagaacaagc
     8521 aagaaatgta atcaaacagt cgtttccatg tgaaactttt tacccagatg aaaataagca
     8581 aaacacgatg gaatggtacg tgaataaaat caaaggagta atcgtatgaa cacgattgaa
     8641 agaaattacg aacaagcaaa agaaaaatat gcaactatcg gtgtagatac agatgctgta
     8701 ctagaaaaga tacaggacat caaaatttct atgcattgct ggcaaggaga tgatgtcaaa
     8761 ggtttcttga caccggatgg cgaattgacc ggtggaatca tggcgacagg gaattttcca
     8821 ggagccgcac gaacacctga agagcttaga caagatctgg agaaggctta ttcgttgata
     8881 ccgggcaaac acaaattgaa ccttcatgct atctatttgg atacagaaga atctgtcgat
     8941 ctaaatgaaa ttgagccgaa acattttgaa aaatgggtag agtgggcaaa aaaagaagaa
     9001 atcggtcttg acttcaatcc gactttcttt tcgcatccaa tgatgaaaga cggctttaca
     9061 ttggctcatc cagataaaga agttcgtgaa tactggatcg aacacgggaa aagaagccgc
     9121 aaaatcgcgg agtactttgg gaaagaaact gggcaaacaa gtatcaataa cttttgggta
     9181 cctgatggga tgaaagataa tccgattgat cgttttaccc cacgggaacg tttaatggaa
     9241 tcactcgatg aaatttttac agaagagtta gatgaacggt atgtacaaga tgcagtagaa
     9301 agcaaactat ttggtttagg cgctgaagct tatacagtcg gttcccatga attttatatg
     9361 ggttacgggt tgactcgtga caaacttgtt tgtctagatg ctggacattt tcatccaaca
     9421 gaagtgattt ccaataaatt atcttcatta gctttattca gcaaaggaat catgttgcat
     9481 gtttcccgtc cagtgcgttg ggattcagac catgtggttt tgatggatga tgaattgcag
     9541 gaaatcgcta aagaattagt ccgtaacgaa ttgttggaaa aaacaaatat cggtctggat
     9601 ttctttgatg caacgatcaa ccgtatcgct gcttgggtca tcggtacgag aaatacacaa
     9661 aaagcactgc tgaaagctat gctggaacca gttgagcgat tgaaagaaat ggagctggct
     9721 tttgacttta cttcaagaat ggcttatacg gaagaattga aagatttccc gtatgccgat
     9781 gtatggaatt atttctgtta caaaaatcaa gttccagttg gtttggattg gctggaagaa
     9841 gtccaggaat atcaaaaaga cgtgttggaa ttaagaaaat aggagtgtgg ctatattgaa
     9901 aaaaatagat attttacaag cgccttatgt gaaagaaatg atcgaaacga cgtcgaatct
     9961 ttatcgcttg ggatgggatg aaagaaatgg cgggaatatt tcttatttac taaaagaaga
    10021 agagatcaca ccgttcttgg atcctgaaaa agtgatccgc cgcatctcga tgatttttga
    10081 tgccagccgt ttagcaggat gctattttat tgtaactggt tcaggaagat attttaaaaa
    10141 tgtagcgagc gaacctgccg agaatcttgg aatcgttcga gttgctgaag atggcaaaac
    10201 actggaactt ttgtgggggc tggaaaacga atgccttcct acaagcgagt tgccaagtca
    10261 tttcatgagt catatcgccc gtttagaagt agacccagat aatcggatcg ttatgcattg
    10321 ccatgctagt catctattag cgatgagttt tacgcatgaa ttagacgaac gttcctttag
    10381 tcgtacgcta tggcaaatgt gtacggaatg cttagttgtt tttccagaag gaatttccat
    10441 cattccgtgg atggttccgg gaacaaatga gattggggaa gcaacagctg aaaaaatgaa
    10501 agaaactcga ttggttttat ggccacaaca tggaatctac gggtcaggaa aagacatgga
    10561 tgaagtcttt ggtttgatcg agacagcaga aaaggcagca gaagtctata cgtacgtcaa
    10621 agcgcaagga ccaatcttgc aaacaatcac ggacgaaaac ctgtggcatt tagcagatgc
    10681 gtttggtgtc actccaaaag caggttattt ggaggaagtt catacgaaag caggtgtctg
    10741 acatggtgaa aaaagctttt tgcatgaaag tttacccaga aaaacaagaa gaatacaaaa
    10801 aaagacatga tgaactttgg ccagaaatgc gtacagcact tcgtgaacac ggggtcatcg
    10861 actattcgat ctatcttgat ccgagtacaa gtttgttatt tggttactta gtgatcgaag
    10921 atgaaaagtt atgggaaaaa atggcaaata catctatcaa tcaaaaatgg tggaagtaca
    10981 tggaagatat catggagaca aatccagatg cctctccagt cagttgggaa ttgatgccag
    11041 tctttgaact gtagcaacaa gcaactgggc aaccggttgc ttggggttct ttcgttttta
    11101 ttgtgaaaaa taatacaaat taggaggaat agaaaatgag taatcgaatg attttaaatg
    11161 aaacatcgta ttttggaaaa ggagcgattg agtcgatcgt ccctgaattt cagcagcgag
    11221 gattcggcaa agcagctgtc attactgaca aaggattgat cgagcacggt atagcaacaa
    11281 aagtaacaga acttctagat aaagcagcaa tcgcttatgc tttatacgac gggatcgtac
    11341 ctaatccaac gatccaaaat gtaaaagatg gagtggcttt tgttaaagag gcagaggcag
    11401 actgtttgat cgcaatcggt ggaggttcgc caatcgatac ggcaaaagcc attgggatta
    11461 ttttgacgaa tccagagttt tcagacgtca ttagtttaga aggtgttgct gatacgaaaa
    11521 atccatgttt acctatatta gctataccaa cgacttctgg aacagcagca gaagtaacga
    11581 tcaactacgt catcacagat gaagtaaatc accgaaaatt tgtttgtgtt gatcctcatg
    11641 atatcccaat cgtcgcgttc atcgacagcg atatgatgat gggaatgccg aaaaaattgg
    11701 ctgcatcaac aggtatggat gctatgacgc atgctattga agggtttatc acaaaaggcg
    11761 cttgggaaat gactgatatg ctgcatttaa aagcaatcga aatcatcggc cattctttgg
    11821 aagcttctgt agatggcgat caaaatgggc gagaaaaaat ggcattagga cagtatattg
    11881 cgggaatggg cttctcaaat gtcggtttag gactggtaca cgggatggct cacccgttaa
    11941 gtgcatggta caacattcct catggcgtag cgtgtgcagc acttttgcca actgtcatga
    12001 aatataacaa agaatatact ggagaaaagt accgtgagat cgctcttgtt ctagggatta
    12061 aaggagcagc tgaaatgtct ttagaagatg taagagaagc ggcttgtggt gagatcgatc
    12121 ggttgagcaa agcggttgga atcccagaaa ccatttctga attaggtgta aaagaagcag
    12181 atattccagc aattgcagaa gatgctttgc gagatgtatg cacaccagga aatccaagag
    12241 aaacgacagt agaagaaatc attgctttat atcaaagttt gatgtaaaaa cgaagcggat
    12301 cttcaacatt aaattgcctg taaagagaaa tggtagttta tatctggata tctcttttaa
    12361 attgatggta tttccgctat aatttttctt ataaagaaat aggagaatat tatatggaaa
    12421 ataaaaagcc ttatggaact gtattgatca aagcctctaa aatactagac tatttagcgg
    12481 aaaaccaaga tgtctctttg cagacgatcg caaagggtgt gggaatgacc tcttctacag
    12541 tcttgaaaat actagatact ttgttgatga tcggctacgt aaataaaaat tcagagaaaa
    12601 actatcgttt aggtgcaaag cttattcgct acgcgaataa aaacattgaa caaatcgatt
    12661 tggtagagtt gactttaccg tttttagaac agctgcagca aaagatcgat gagactatcc
    12721 acttaggggt attggacaac aatgagatct tgtacgtgaa caaactagat ccaaaacatc
    12781 aaacgatccg catgtcttca aaaatcggaa tcacccgacc attatacaat tctgcgatgg
    12841 gcaaagccgt attagcagaa ttcagtgaag agcagtacac tgattatctt gaaaagcatc
    12901 cgttgatccc ttacacagaa tatacgatca ccaatgcatt acggttgaaa aaggaaatcc
    12961 aaacggtaaa agaaacccac atcgcatttg acgatgaaga aatcgaacgt gatatttttt
    13021 gtataggagc ctcaatcgtg aaaaacgaac aaatcattgg tgcatttagt gtaagtatgc
    13081 cgaaatatcg tttaacagat gagacaaaag aacaaataat cagtgccctc aagcaaacaa
    13141 aaagcgaaat cgagcaaaga attcaaaaat aatgaaaaaa gcgtttgcaa tttttctttt
    13201 ctgccgtata atatgggtat agatttccaa gttataaaat aaaatttcta aataggaaat
    13261 aggtggagaa gatgaaaaaa attgatattt tatctcgatt gaaaaacgcc ggagtgattg
    13321 cggtggtacg cggaaaaagc aaagaagaag ctttgaatgc ttgccacgca atcatcaaag
    13381 gtggtctaac tggtattgag ttgactttta cagtccctca agcagatcaa gtaatcaaag
    13441 aacttttgtc attctacaaa gaccaaccag aaatagtgat tggcgcaggc acagtattag
    13501 acgctgtcac agctcgcttg gctatcttag ctggcgcaga gtatattgtt agcccttcct
    13561 ttgatcaaga aacggcagag atgtgcaatc tgtatcaaat tccttactta ccaggatgta
    13621 tgactattac cgagatcaaa acagcgctga aaagcggggt agatatcgtc aaactatttc
    13681 ctggaagcgc ttatggacca agtatcattt ctgccttcaa ggcgccgatg cctcaagtca
    13741 atatcatgcc aactggcggt gtgagtctgg acaatatgaa agagtggttc gatgctggtg
    13801 tcgtaacagt cggagtaggt gggaaccttt tagcacccgc agctacagga gattttgaca
    13861 aagtaacgga agtcgcgcaa cagtatgcag caaaaatgaa agaaatcaag aggtagctgc
    13921 ttatgggaaa agtagtgaca ctcggagaaa tcatgttgcg attgtccaca gagcagggaa
    13981 cccgtatcga ttcaagtgaa cgattcgctg cccattatgg cggaggggaa gcaaacgtag
    14041 caatttcttt agctaattac ggacatgaag cgatttttgc tacaaaagta cctgataacg
    14101 cattgggaaa agcagtcaaa aagcacttgc aatgttatgg agtagatacg aaacaggtat
    14161 tatctggtgg atcaagacta ggtacttatt atatggaaac tggtagtggc gaacgcgccg
    14221 cctcagtgat ctatgatcgt gccggatcaa gttttgctga aatggatcat ttggaatggt
    14281 cgctagagga gttattccaa ggaatagata tatttcatgt ttcaggaatc accccagctt
    14341 tgtctaaaaa atggcaaaaa atgacgaaac aggtgataga agctgcaaaa aaagctggct
    14401 gcctgatcag cttcgatatc aactatcgtg gaaaattatg gacacagcaa gaagccagcg
    14461 aagtaatcca tcagctttta cctttagtag atatctgctc agctggaaaa atggatgctc
    14521 tttatttgtt aggtatcgag aaagcgccag aagcagaaga acaacctcta atttattatt
    14581 atcaaaaaat gcaggaaaag ttcccgaata ttcaggtgtt ttattcgacc aagcgtaccg
    14641 ttcattcagc aagtgaaaat gaattgaagg ggacattgtg gatgaaccat gaatatgtgg
    14701 aatcccaagt ccatagaatc accccaatcg tggatcgagt aggtggcgga gatgcttttg
    14761 caggtggcgt gctccatggg attttatcca atatgtctcc gcaagaaatc attgattttg
    14821 ctacggcagc ttctgccttg aagcatacga tccatgggga ctgcaaccaa tttagtcaag
    14881 aagaagtcgc acaattttta gcatgcggct ctggaaaaat cattcgatag aaggagtaga
    14941 agaaaatgca agaaatggaa acacgttata cacacagtcc agaagatatc cgtcattatt
    15001 caacggaaca attgagaaaa gaatttttag tagaaaaggt atttgaacca gggaaaatca
    15061 gtttgactta tacgcacaac gatcgaatga tctttggtgg agtgacacct actgaaaaag
    15121 aattagaaat cacattagac aaagaactag gtgttgatta cttcttagag cgtcgtgaac
    15181 taggtgtgat caatatcggt ggaccaggat tcatcgagat cgatgggcaa aaagaagaaa
    15241 tgaaaaaaca agacggatac tatatcggaa aagaaacacg acatgttgta ttttcttctc
    15301 aagatgctga aaacccagct aaattctata ttagttctgt tccagctcat cataaatatc
    15361 caaatgtaaa aatcagtatc gaccaagtaa aaccaatgga gactggtgaa ggcctgacac
    15421 tgaatgaacg taaaatctac caatacatcc atccaaatgt atgcgaaagc tgtcaattac
    15481 aaatgggtta tacgatttta gaaccaggaa gcgcttggaa tacgatgcct tgtcatacac
    15541 atgaacgtcg tatggaagct tatgtctact ttgattatgc caatgaagat acacgtgtgt
    15601 tccacatgat gggcaaacca gatgaaacaa aacatttagt tgtagacaat gaacaagcca
    15661 ttatttcgcc aagctggtcg atccattcag gtgtaggtac aagcaattac tcattcatct
    15721 gggcaatgtg cggagaaaat atcacctata cagacatgga tatggtgcaa atggatcaat
    15781 taaaataagc ataagggaga gaaaatgatg gaatttagta tggatatgtt tcgtttagat
    15841 ggaaaagtag cattagtcac cggtgctgtc tatggtattg gatttgagat tgcacgctct
    15901 ttagctacag caggagcaaa aatcgtattt aataacttga atcaagaatc agttgatgaa
    15961 ggtatagcgc attataaaga agcaggcatc gatgcaaaag gttatgtctg cgatgtgacc
    16021 gatgaagaag cggttcaagc gatggttcaa cagatcaaag aagatgtagg ttcagttgat
    16081 attctagtca acaatgcagg gatcatcaaa cggacaccga tgatcgaaat ggatgcggca
    16141 gacttccgcc aagtaatcga cgtggattta aatgccccat tcatcgtttc aaaagcagtt
    16201 attccggata tgatcgaaaa aggcggcggt aaaatcatca atatctgctc catgatgagt
    16261 gaattaggac gcgaaacggt cagtgcttat gctgcggcaa aaggcggatt aaaaatgctg
    16321 acgaaaaaca tcgcttctga atacggtcaa tacaatatcc aatgtaatgg aattggacca
    16381 ggttatattg ctacaccgca aactgctcca ttacgcgaaa cacaagaaaa cggtgaacgt
    16441 catccattcg atcaatttat cgttggacgt acgccagcag cacgctgggg cgagccaatc
    16501 gatttggctg gaccatctgt cttcttggct tctagcgcat ctgatttcgt taacggccat
    16561 gtcttgtatg tggacggagg catacttgcc tatatcggaa aacaacctta aaagagcttt
    16621 agtacactgt ttagtggaga ggttgtgaaa tcagcggatt gaacgaatac cgaaggacag
    16681 ctgattaaac atcatttatt cgaaaaaaga ggctgggaca gaagc
//
LOCUS       DACEOE010000047        16565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEOE010000047 DACEOE010000000
VERSION     DACEOE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.049"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..644
                     /locus_tag="IW774_001283"
     CDS             <1..644
                     /locus_tag="IW774_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HAQ1153637.1"
                     /translation="IKKLGIKVTTYTRKSRRYNSYRGQVGAVAKNRIHRRFYTSICHQ
                     KITTDTTEFKYHEVDAKGIIRQKKLYLDPFMDMFNSEILSYRISEKPNALAVMEGLEE
                     AIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGL
                     LKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQSLVQFRKTTVTVV"
     gene            833..1501
                     /locus_tag="IW774_001284"
     CDS             833..1501
                     /locus_tag="IW774_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1153638.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1701..2087)
                     /locus_tag="IW774_001285"
     CDS             complement(1701..2087)
                     /locus_tag="IW774_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153639.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(2059..3441)
                     /locus_tag="IW774_001286"
     CDS             complement(2059..3441)
                     /locus_tag="IW774_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1153640.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3597..4784)
                     /locus_tag="IW774_001287"
     CDS             complement(3597..4784)
                     /locus_tag="IW774_001287"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1153641.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4791..5822)
                     /locus_tag="IW774_001288"
     CDS             complement(4791..5822)
                     /locus_tag="IW774_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1153642.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5838..7292)
                     /locus_tag="IW774_001289"
     CDS             complement(5838..7292)
                     /locus_tag="IW774_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1153643.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            7467..8372
                     /locus_tag="IW774_001290"
     CDS             7467..8372
                     /locus_tag="IW774_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1153644.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTTVSWTT"
     gene            8463..9140
                     /locus_tag="IW774_001291"
     CDS             8463..9140
                     /locus_tag="IW774_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1153645.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9421..10317
                     /locus_tag="IW774_001292"
     CDS             9421..10317
                     /locus_tag="IW774_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1153646.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10372..11742)
                     /locus_tag="IW774_001293"
     CDS             complement(10372..11742)
                     /locus_tag="IW774_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1153647.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11980..12726
                     /locus_tag="IW774_001294"
     CDS             11980..12726
                     /locus_tag="IW774_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153648.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12723..13562
                     /locus_tag="IW774_001295"
     CDS             12723..13562
                     /locus_tag="IW774_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1153649.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13563..14513
                     /locus_tag="IW774_001296"
     CDS             13563..14513
                     /locus_tag="IW774_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1153650.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14654..15406
                     /locus_tag="IW774_001297"
     CDS             14654..15406
                     /locus_tag="IW774_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153651.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKNADQQIQFVQLKKEKLYYGLYSR"
     gene            15425..>16565
                     /gene="queG"
                     /locus_tag="IW774_001298"
     CDS             15425..>16565
                     /gene="queG"
                     /locus_tag="IW774_001298"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1153652.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRR"
BASE COUNT         5389 a         3104 c         3335 g         4737 t
ORIGIN      
        1 tgatcaagaa gctaggaata aaagtaacga catacacacg taaatcacgg cgctacaatt
       61 cttatcgagg acaagtcgga gcagtagcca aaaatcggat ccatcgtcgt ttctacacca
      121 gtatctgcca tcagaaaatc acgacggata caactgaatt taaataccat gaagtggatg
      181 ccaaaggcat tatccgtcaa aagaaattgt atctagatcc ttttatggat atgttcaata
      241 gtgagatttt atcctatcgt atctctgaaa agcccaatgc attagcggta atggaaggtt
      301 tggaggaagc tatccagacc actaacgatt gtccctatcg tcgtacgttt cactcagatc
      361 aaggttgggc gtatcaaatg aaagcttata gaaacaaatt gaaggagtac aaaatctttc
      421 aaagcatgtc tcggaaaggg aactgcttag ataattcact tatggagaat ttttttggct
      481 tattaaaaca agagattttt cacgggaaag tttataactg tttcgaggaa ttaaaatcag
      541 ccatcgatag ctatatctat tactacaata atgaacggat aaaacagaaa ttaaactggc
      601 aaagtctcgt ccaatttcgt aaaacaaccg taacggttgt gtaaaaatat cattttagag
      661 cataaaaaaa aggagtggat ttctccactc caaaaaagtc taacttttgg gggtcactac
      721 agtgtcacag tccttttttt agtaaaagga tacatttctt ccttgtatat tccatgcgtg
      781 ttcgtgagaa agcatatata atttaattaa cagatagtta gggggagaaa aaatgatttt
      841 ttcaattaca atgaaactgg tgttcgggtt gatcggttta ttattagtgg tacgtttgct
      901 tgggaagaaa tccatgtcag aaatcacacc gtttgattta gtctatacgc ttgttttagg
      961 tgggatctta gaagaatcaa cctatgatga caatgtccat gtagggcatg tcctatttgc
     1021 cattgcgctt tgggcagtaa tgatttatgg tatcgaacgt atcgtccaaa aaaatgaaaa
     1081 ggtcaatcgt tgggtcaaag gcgagccatc tgttctaatc aaagacggcg tcatcaacat
     1141 gacagagttg acaaataacc atatcgagat ggagcagttg cgagcaatct taagacagca
     1201 agaatgcttt tttcttgaaa atgccaaaca tgttatccta gagaatgccg gtcaaatgag
     1261 tgtattgaaa aaatcagatg aagacaaagc tttatcgatc ctgcttgtag atgaagggca
     1321 aattcagcat aaagtgcttc agtccaatca attgactgaa gcatggctta tggagaactt
     1381 gaagaaagaa ggctatgcag atgtaaaaca gctgatttat gttgaatggt cagaagaaaa
     1441 agggctctac atactaaata aagaagacgt catgcatgaa tcttaccgaa tagatggata
     1501 gatagacaga ggttgtgaaa tcagcgcctt ggcttgttac tcaaaactaa gcgacttttt
     1561 cacaacctct gatatctaat atacgttgtt ccagaagttg cttcttcgaa aaataagcca
     1621 aaattccaca aaaatttaag aagcaatttt cgaaaaatcc tttcttattt tttgaagttg
     1681 atcacttctg tcacaacctc tcatgtcaca aaccattttt ttgtttcctt taactcaata
     1741 taccgtacgt cttcccacaa tatcggctga tggtcaatca taaagtaatc ttcataggct
     1801 tcaccaatga agtgcccttc gacctcatcc aagagtcttc caaattcatc ttttaaattg
     1861 agctgaatga ttactgcttt acgagataaa aaggcttctg tcagtatact atcgatttct
     1921 tgcaatgtca tttgttgttt aggaagaggc tgtttgcgtg cttcttcttc attttttttg
     1981 ataccgttga ctagttcttc catcgcaaaa gcagtccccc attttaatcc aaatggccga
     2041 tctcgatact cgttatagtc aatgaatttt tttgctgtcc gtctcatctt ttccaccttc
     2101 taatccactc ataccacctg catgcccgcc aacgagagaa ctacgtgcga tggcgcgtcc
     2161 tccttgcaag agactatgcg catggatgat tgattgaaag ccgtattttt ttctaatcgt
     2221 atcgacaaca aaatcaactt tcgcttcatt tatctgcttt tcaggatctt caaaaaggtt
     2281 aagctgtaaa cctgtcgcgt atacgaggtc ggaacagttc acgcctacac gccgaatatc
     2341 ctggtagtgg taatgctggt ggaaaatggt caataaagca tctgctagat ctctgctagc
     2401 atttgtcgct tcgattttca tttgctggtg aaacccggtc ttgccatttg gatcaagata
     2461 gcctaaagag tatcctaccc ataagctgac tacttgtgtt tttgctgcag atcgtctcaa
     2521 gcgagtgccg acttggtcgg ccatttcttt gataacgatc tcgatttctt ctcgtcttgt
     2581 ataatctcta tctaggattt gactatttcc gatacttttt gattttggtg tatattcttg
     2641 tcccaaaaaa cttcgatcga tgccccaagc atgggcataa agctgagtac cgatgacccc
     2701 tagcttgctt ttcaagtgat aataatccgc gtgagctaaa tcataaacag aatgtatacc
     2761 catttgtttc agacgttttg ctgttcgttt gccgattccc caaaagtcag tgatatctga
     2821 gattttccat aacttttctg gtacatcttc ataacgccat tcagcaaaca tttgctttcc
     2881 gtcttttgag ccattgtcta aggctagttt agcaagtagc gggttatcgc caattcctat
     2941 agtcgtgtag attccggtct gctcaaacac atctttatgg atcagtttag ccatgtcgta
     3001 cgcatttgtt ttaccaaaga gtgccatgct atttgttaca tcaaggaagc tttcatcgac
     3061 ggaatagaca tgatggttcg cttcgtctgc atatttttta tagatagaat tgatttctct
     3121 gttcttttcc atataatagg ccatccttgg ggcaacaata tataaatcac ttggataggg
     3181 gaagggaaga tctctggcac ggctgatatt ggtgatgccg taagcttttt ttgctgcagg
     3241 actggaagcc aagattaatc cgctaccccg tgtttctgtc gaatcggacg gataggacat
     3301 gactactaat ttagttgtaa ggggatcaag atccttttca acgcactcta cggaggcgta
     3361 aaaagatttg cagtcaatac ataaaatatc tcttgaaggt tctttttcat aaacaaatac
     3421 cggattttga ttcgcccaca taacaatcac ctcttcaaca caattagaac acacgttctc
     3481 tttccgtgtc aataaaaaac agcaattttc aaatacgagc aaaaggctca catttggaaa
     3541 ttgctgagag gttattttta tctatttgtt tttagctgag atcgttgatt tttttatcaa
     3601 acatgaacag cttctggatc gatctttgtt gtcccatttc caatgtgtgc aacataaaat
     3661 tccggagcat agccgacaac tttcatgtat tcatcataga cattgttttt gaaggcaggt
     3721 atattttctt ctttgacaag agcaatcgca cagccaccaa atccagctcc tgtcattcgt
     3781 gcacctaaga caccttcttg tttttgagca gcatctacta gtgtatctaa ttcgatccca
     3841 gttacttcat aatcatagcg aagagagtgg tgagaatcat ttaacagttg accaaatgct
     3901 tctaagttcc cagcttctaa ttcagctttt gccttcaaag tccgctgatt ttcagaaacg
     3961 gcatggcgag ctctgcgtat caatgtatca tcgccgattt ggtcaatagt tgattcaaat
     4021 tcttcttcat ctaagtctcc taaagaagag ataggcaaag ttttttgcaa acgtgccaag
     4081 gcttcttcgc attcgctacg gcgttcattg tattttgaat ctgccaattc ccgccgctta
     4141 ttcgtattca tgatgacgat tgcataacca tctagtttca ctggaaccat ttcgtatttc
     4201 agcgtatttg tatctaataa gattgcatga tctagctcac cgaagccgat ggcaaactga
     4261 tccatgatac ctgaattgac accgatgaat tcattttcta cttttttacc tgtttggact
     4321 agttccaagc gatctgtttt taaatcaaac agatcttcta atacaacacc tactaataat
     4381 tctaatgaag cgctactgga aagtcctgca ccattaggga ttgttccttc cactaataac
     4441 tcaaacccat gatcgattga gtgtccagct tcttttaatt taaggaccat tccttttacg
     4501 taatttgccc agtttgcttt tttatcatat tctagctcat ctaaagtaaa actaatgaca
     4561 ccgatatcgt caaagtttgt ggaataaact aatattttat tatcgtctcg aggtgctgct
     4621 acaccttgcg ttccataagt gatggaagcg gggaagacat ggcctccatt gtaatctgtg
     4681 tgttcaccaa tcaaattgat gcgtcctggt gagaagtatt gtgtagtttt tttaggtccg
     4741 aatgtttcgg caaattttgt gtttaagctt cttttgattt ccatgatgag ttattccttt
     4801 cttgttttga ttttgaattc tgtcactgtt tcaaatactg atccggcttt taaatgaatc
     4861 gaaccaaatg ctgagaagcg ctctgcacct ggagctgttt gagtctcaaa tgtgatgcca
     4921 ccgtgatgtg ccagttttcg gttgcgcatt tctggtgttt ctgtgccgaa atttgcagtg
     4981 aaaataacca cactagaagc atctgtagca atatctactt ggattctttt atcggggcta
     5041 gtcaagcgag cggcttcttt acccaagccg gtttctttta agaaaaatgg atgatcgatt
     5101 ccgtcaacaa gatttttttg atccaaatca ctagcaaaaa cggacgctaa ttttttaggt
     5161 atttgaaaat cgaaagcact tcctgcagca ttttctttta cccctatagg aatactatct
     5221 gttcgcaaag gagcataagc ttcgctattg agccaaagct catgttgatc gattgactga
     5281 gaagcatctc ccgtcaagtt gaagtaaaca tgatttgtcg gattgaataa agtatcttgg
     5341 tcactggtgc cgcgagaggt cactcgccag atattatctt ttgtcaaagt atagcggatt
     5401 tccacatcca ttgtacctgg aaaaccatgt tctccgtcgg gacttgtagt agtaaaaatg
     5461 acagaagctt cattttcgcc attcaaaatg acataagacc atttttttaa ttcaaatcca
     5521 ggagcaccac catgcagatt atgtccagtt tttggatctg tggctaattg gtaagttttt
     5581 ccattgagag aaaagtggcc attttcgatt ctccctgcgg tgcgaccgat agaagcaccg
     5641 atatacggat ctttttcgat atattcttca gcggtgtcaa atcccaaaac taattctcga
     5701 tcatttgatt ttaaactgac gattctagca cctaaatcag agatagaaag tgctagcttg
     5761 tttttgttct ccaaggttat caagtgataa ccttggccaa aatctttttc tgtaatcttc
     5821 attttcaaga tcctttctta atgggaagga tctactgcat cgtgaagttc atcttccatt
     5881 gtatcagctg gagccaattt gttttccaat ttctcaacga tttgtgcatg ttttttctct
     5941 gtcaatgtaa tcttccaagc aaaaataata gcggagaaaa tcatcaaagc agcaggagca
     6001 tagaatgcat aagctttgaa agtcgttacg ccatgagcag tgatatcagc agcagtagca
     6061 ttaccagtca tcccagaagc gatagcgaca aaccctacga tcccgttaga caaagcacca
     6121 gcgattttat ctagtaaagg gcgaattgat agagtaacag cttcgttacg gataccattt
     6181 ttccattgac catattcaac agagtctgtg atcgtcatca aagcggataa gaaaatcaat
     6241 tgttgaggga agtagaacaa tactagcccg atgataacga ttggcaaact tgaacctgcg
     6301 atcgtaaaga aaaagtaaga agaaatcatc attgcgatcc ctccaaggta aacgtagcga
     6361 cgagagatga cgcgtgtaag cattgggaat agtggaacag ctactaaacc aagtaaggca
     6421 gcaactagac caacgatcca aaattcagtt ggttggccaa gtacatattt gaagaagtag
     6481 aataatacgg cagttgttgc tgcattggca atcgcataag cgatataagc aagggcaagc
     6541 cacatcaact gatcattttg agcaactgct ttgaacacat ctttgatccc agctttttca
     6601 ttttgctgac ggatcgcagt atcgacttct tttgtgccca aggcagtgat aatcgcagta
     6661 ccgcctgaaa taacagcagc aatcacaccg aaccagaacc agcctgaagc gccttcaata
     6721 tgtttatgag taacaagata ggtaaagaaa gtaacgatag ggacaacgac tagagtagta
     6781 ccatttgcac ctagagaaga gccgaaacga gcaaacgtag caaatttttc acgttccttt
     6841 gtttccgtac tcaaagctgg aatcattgac caaaaagcga tatccttaaa tgaataaaaa
     6901 ctatccaata agatgaaaac gattgcaaat aatatgataa ataaagtcgt attatgagta
     6961 gctaagccaa agaaattggt aaagattgct accaagagta cggaactaac aacgccgccg
     7021 ataactaacc atggtttgaa tttaccgtat tttgtacgag tattatcgat gatgccacca
     7081 atcaaaggat caaaaataat ttcaactaag cggataccta caactaagct tgtaactgta
     7141 gcaatcattt ttgcattcgt tgccttatct gttcctgcga acatctggct tgtcacgaat
     7201 accataaaat aagtgcttaa tgtaacatag aatgtgtcgt gtccaaaagc gcctaaagcg
     7261 taggacacac gttggatcat tttgcttttc ataatctgta ttcctctcct gttcaatata
     7321 taatactaag taaaccggtt acaacgaata acaaagatct tttaatgact taaacttaac
     7381 aagaaatcgc ttccttttta atatatttct gtgactttta tttaactttt tgttgcatta
     7441 tactaaagga gtaaggaggg atagacgtgg agcaatcgat ttttttaaaa aaggaacatc
     7501 aacaagtaga aaaagaattg tattttgatt tttgtggttt ctctaaaacg atgccgtttc
     7561 attcttttgg tcctgctatt cgcaatgatt acatcctgca tgtcgtgatg gaaggcaaag
     7621 gatcttatca tgtgaaaaat caacaattcc aattgaaaaa aggtgatctg ttcttgattc
     7681 gtccaggaga ctcgacgttt tacttatcag atggagaaga tccgtggatg tattgctggc
     7741 tttcatttgg tgggagtgct gcagaagaaa tcattcggca gtcgcttttc agagaagacc
     7801 aatacacgat ggtctcttct gacatacaaa aatatatcga cattatttta gaatgcatga
     7861 aagatacgga agaaaccttg gaaaatgaat tacagttaac agcattaacc taccgttttt
     7921 tatcagtcct tttaaaagat ggcggaaagg tcagcggtgg tgtacaaaag gttcattcct
     7981 ctttagctgt tgaaacagtc aaatacatcg aggcacatta tgctgaaaaa ttgtctgttg
     8041 aggaaatcgc ccagcattta gcggtcaatc ggagccattt gtcgcgagta ttcaaaaatc
     8101 atatgggtac cagtatcaag gaatatttga tcggggtacg gatcaatcga ggggcgtttc
     8161 ttctttctct gacggatgaa tccgtggaaa gtattgccta tcaagtaggg ttcaatagtc
     8221 tagtcgtatt tagccgaatg ttcaaaaaga gtaccggaga aacggctacg aactatcgta
     8281 aacgtatgaa aaacaaaaat tttaagaata ttgctttgga taccttgaaa aaacagttgg
     8341 aaaaacaaac cacagtttct tggacaacct gattagtctg gttaaaatga caagcatcgg
     8401 caaaagaaac ttgtggcggt tatgctacac tgtaaagcga agactaaaaa ggaggaaaag
     8461 ggatgcgtac acttatcatc attagccatc caacgatcga agaatcttta aatcagcaat
     8521 ttttcaaaga agcttctgct ggtttaccag ctacttggca tcatttggaa tcaagttatc
     8581 cagacggaaa aatagatata gagactgaac tacagctctt ggcccagcat gatcggatca
     8641 ttttccagtt tccgttttac tggtatagtt ctcctgctca tttaaaaatt tggcaagata
     8701 atgtcttgga acgagcagca catgtattaa aagggaaaga actaggaatc gtattgacaa
     8761 caggtgtggc ggaaaaagaa taccaagctg gaggaaaaga agaatatacg atttccgaat
     8821 ttttgaggcc gtatcagcga atcgcaaata aattccatat gacctatctt ccgccattcg
     8881 ttttagcaca atttatgtat ttatcccagg aaaaacgttg ggagaagcta attgcttatc
     8941 agcagtatct ctcattggaa ggcaaaccat cgctgactca gcggattgac tggtttattc
     9001 aaagagtaca ggaaaatcag aaaatgcagg aagaagaatc tgaaaaacaa gcatatatcg
     9061 tagaagcact gactgaggca aaagagcaga tcgaagactt gacctttaca ctgcaagaaa
     9121 tgaaaggaac ttcattatga caaatgattg gctgatccaa caattagatg aattaagcga
     9181 aaaatcagaa tatcaagacc gtgcgctctt atatgaaata aagaaactga tggctgagat
     9241 agacgaacga acaatgcagc tgcaaggaga aatcgacgga caatcttgga atcatctcaa
     9301 ctggtgaaat tttcttataa ataacaaaga taaaaatgtg cacagcgaca attatttgct
     9361 acaataagaa tatgaagcgt ttgataacgc tattatacat aaaaattggt ggtgaaaaaa
     9421 atgtatgatg aaaatgagtg gggagaaaat gttcggatga cccgaagaag acagcaaaaa
     9481 caggcagctc gtaagcggat gatcatgatt ggggcagcag ttgccgtgtt tttgatcata
     9541 gcaggaggag ttgcgggtct atattttcat ggaaaaaatt cgtcagaagc gaaaagtacg
     9601 accataacgg aaacaacgac aaagaaaaca gagacgaaac aaacaagtga gtcatcaact
     9661 aaaacaaaag agacacaaac ttctgaagaa aaaacagcaa atggtttgcc agagcaactt
     9721 caagcaatcg taaatgatta ttcgaaaaag atcgaagaaa agacgcctgc attgattgaa
     9781 gaataccaga cagaaattca gggaaatcaa gagggaatag ctggtttatc agcagttgct
     9841 aaccaaaaag cacgtgagct tcaagcaatc tccgacgaag ggatcagaaa attacgtgca
     9901 gcgtatcagg cagcagaaaa taaagacggt gtagacttag atactttgat caatcaatta
     9961 tcggctaatt atacgaatca tgtcgctaag atttccgata tttatttgca gactagcgcc
    10021 tctcttcaag cagagtcaac aagcacacaa gacacaacat cttctgattc tgagacagca
    10081 gagtcggtag aagcttctga aatgcaagct cgaacaacac aagacacaac agattcctct
    10141 gaaactaata ccaacagcgc gacttcacag gctgcgacaa ccgtagtttt ggaaggagaa
    10201 ggtccaaatc aaatagccga acgaactggc gtaccagtag aaacgatctt gtcgctgaat
    10261 gggatgagca tggagaatta tttcctgact cccggtgaga cgctaaaatt gaattgataa
    10321 aagcaataaa aaagaacgaa gcttccacta aggtgaagct tcgttctttt tttattttcc
    10381 ggttcttttt tcgcgtacaa agtaatggat cgtcatgtaa agcaagaaaa cgagtccaat
    10441 aatcaatgag attctcgttt caggattgat aaacatgaaa atcaatacga tagctaaaaa
    10501 agcaagtgtc acataattcg tataaggaga tagtggcatt ttaaaaggat gatcatgcat
    10561 tttatctgcg tgcttctttt tgaagcgaat ctcgctgatc aaaatcacta cccaaggaac
    10621 cataccaggc aaaatacttg agctgtatac gaaaacgaaa atattttctg actggttgat
    10681 gaacattggt agaagataat ttaagatcag accaattaag attccacctg agattgctac
    10741 gacaggataa aaaggcgcac cgtgacgaga tagtttcaga aattttttag acatctttcc
    10801 atttatggca agtgtataga ccatacgact tgcgctgaaa atccctgaat tgcaacctga
    10861 taatgccgct gtaatcacga caaaattaat cagtcctgca gcaaatgtaa tgccgacttt
    10921 agaaaaagtt tgtacaaaag gtgaaccaat ctcactcaat tgattccacg gatagatact
    10981 cacaatgata aaaatagcac caatataaaa aatcaagatt cgaccgatag tggactggac
    11041 agcttcaatg atcgtctttt taggattctc tgcttcacca gcagtcattc cgatcagttc
    11101 gattccttga taggaagcaa caacgatcga taatgcaaag aaaaatcctt tgacgcctcc
    11161 agtgaagaat ccaccatttt tccaaagatt tgaaatccct actgcctgac catgattacc
    11221 aaatccaaat aagattaccc caagacctgc aatgatcatc aaaatgatcg ttatgacttt
    11281 gatgatcgaa aaccaaaatt ctaattcgcc aaacgccttg acagaaataa gatttgccag
    11341 cataagaaac aagacgacaa tgattccggg aataattgcc ggcaaatcag gccaccaata
    11401 attcatataa gatcctacag caatcacttc acttattcct acgacaatat attgaaaaat
    11461 attactccaa gcggtcaagt agccagccac aggatggatg tactcgctcg cgtaatttgc
    11521 aaaagaacca gtcgaaggat caacgtaaag catttcgcct aaagcacgca taatcaaata
    11581 caaaaagaat cctgcaatcg cataggctcc catcactgag ggccctgtcc aacgtatggt
    11641 tgaggaggca cccataaaca gaccgacacc gatcgttcca cctagtgcga tcatttggat
    11701 atgacgggca ctaagacttc gagttaactt ttcttcttcc atacaaaaac tcctttcgtt
    11761 gttgagtcaa caagaaaaaa taatcaaacg tttcaacaca tgtttttcat tattaggtta
    11821 gccttattga actcttgatg tgattctaca gaaaattttc ataaaatgcg aggaaaattc
    11881 gaaaaaaact ttttcatcat tctacttgaa aatgctttcc attttagtta aactatattt
    11941 aaataaaaag ttaggtaaac ctaaaaagga gtgtaagatt tggaacgtac ggccattgta
    12001 attcaagacc tttcagccgc ttatcaaggg aaaactgttt tgaaaactat caacttgacg
    12061 ataaatccac aaaaaattac cggtattatc ggaccaaacg gtgcaggaaa atcaacattt
    12121 atgaaatctt tgctagagtt gataccagct actactggta aagtcacctt ttctggacag
    12181 tcggtaaact cagtccgtaa aaaaatagcc tacgtggaac agcgaagtga attggatctt
    12241 tcctttccaa ttgatgtatt aggcgttgta cttttaggaa catatccatc tttacgaatt
    12301 ggacaaagac ctgggaaaaa agaaaaagaa cgtgcaagac aagctttgaa aaaagtaggg
    12361 atggaagaat atgccaaaag acagatcagc gaactatcgg gtggacagct tcagagagtt
    12421 tttattgcaa gagctctagc ccaaggagca gaatggatct ttttagatga accattcgta
    12481 gggattgatg cgttaagtga acgaaagatc tttgacatct tgcaggaatt gaagaattca
    12541 ggaaaaacga ttttgatcgt ccatcatgat cttcataaag tagaagaata tttcgatgag
    12601 gttattcttt taaataaaca gctgatcgct tccggtccag tacaagaagt ctttacatcg
    12661 aagaaccttc aattggccta tggtgagatc atcagacatt tagtaaaagg aggagagaaa
    12721 aaatgatcca atcctttatt gatggattaa tgaattatca attcctgcaa tacgctttgg
    12781 tcacttccat gcttgtagga cttgcttctg gagtcatcgg ttcttttatt attttacgag
    12841 ggatgtcttt gatgggagac gctatctctc acgcagttct tccaggtgta gccatttctt
    12901 atatgtttgg ttttagttac atctttggag ccactgcttt tgggatgcta acagcagcag
    12961 tgattggttt cgtgactcag cggagccgat tgaaaaatga tacagcgatt ggaattgtct
    13021 ttagctcctt ttttgcatta gggattatct tgatttcata tgcccagagt gcgacagatt
    13081 tgtatcatat tttatttggg aatgtcttag ctgttaggga aagcgatttg ttgctgacag
    13141 cactcgttag tggaatcgtc ttgatatttg tctttttctt ttataaagaa ttgaagatca
    13201 catcattcga tccgacaatg gcaaaagctt atgggctgaa cacatcgctg atccattatc
    13261 ttttgatgtt ctttttgaca ttagtggctg tagtcagttt acagacagta ggaacaatct
    13321 tggtgattgc catgttgatc acaccagccg ccacggctta cttgctaacg aaccatttac
    13381 tgaaaatgat cattacagct gcaggaatcg gtatgctaag tgcagttgtc ggtgtgtttt
    13441 tcagttatag ttacaattgg ccatcaggag ctacgatcgt gttagcatgt accgcatttt
    13501 ttatccttgc ttttttattt tctccaacaa aaggaatttt atttcagaaa ggaagcagat
    13561 agatgaaaac aaaaaaatca ctctttttaa tcttggcagt tagtttcttg gtattagccg
    13621 gttgcggaaa gcaggcttct gaccaagcgg atgaaggaag taaagaaaag ttgtcagtcg
    13681 tggctaccaa ttcgatcttg gcggacatgg caaaagaggt cggaacagat caaatagata
    13741 tccacagtat cgtaccagtc ggaacagatc cgcatgaata tgaagtacta ccagaagaca
    13801 tcaaaaaggc aagcgatgca gatgttattt tatacaacgg tttgaatctt gaaacaggta
    13861 acagctggtt cgataacttg atggaaacgg ctaaaaaaga agaaggaaaa gattattttg
    13921 cagttagcaa aaatgtagaa cctctttatt taactagcgg tgaagaacat acaaaagcag
    13981 atccccatgc ttggctagac ctatctaacg gaataaaata tgtggaggaa atcgcacgta
    14041 tattctctga aaaagatgca gaaaatgcga cactctataa aaaaaatgca gaagcatatg
    14101 tggaaaaact aaaagaatta gatacacaag cgaaggaaag ttttgcttct atcgaagaga
    14161 acaaaaaatt attagtaaca agtgaagggg cattcaagta tttttcacga gcatatgatt
    14221 tgccagcagc ttatatatgg gagatcaata cagaaagtca aggtacgcct gatcaaatga
    14281 aagcgatcat tgatcagata agagcaaaag aagtaccggt tttattcgtg gaaaccagtg
    14341 tagactcaag aagcatggaa cgggtagcaa aagaaacggg tttgaaaatc tatgataaac
    14401 tgttcactga ttccatagca aaagaaggag aacaaggaga ttcttactat cagatgatga
    14461 aatggaatat tgaaacgatc catgaaggtt tgtcacaaac aaaagaaagc tgaaaagtgc
    14521 aaggtctagc ttgaggagca gaccttgcat tttttataag agatatgaaa aaaaattatg
    14581 ctgtttttcg tcgtacagat accttttttg aagaaattcc gttatagtta gagtaaggaa
    14641 ggaaggtgtg gctatgaatt ttacacgtaa agataaatat acgattaaag aagaagtctt
    14701 tcgattgatc agccagcagg aaaataccga caaggatcaa gaccttcaat ttcttgaagc
    14761 agaattagct aaattattca tagcaaatca aaatgagaca acagaaccag atgaagagct
    14821 gtacataggc aaatggttgt ttgatgaatt tcagccttta gatgaaaata ccggtatcgg
    14881 ttattttgtg ttcctttctc cagtcggctt cacacctggc gtattagtta aacaaaacga
    14941 cgaaataacc gtcaaagcat ggcctgcatt attttcagtc atcaaagcag aaggagtaat
    15001 caaaaagatc cagtcggcct attataaaag tcaatttatt gccaacagca aattaatgaa
    15061 tatgttttat aatcgtctag tagaaattgc taggcctggg aaaatcccgc aaacattcac
    15121 gtacacacag caaaaaaatc tctatgaaga agtattgtat tcccgcgatc tgtctgagtt
    15181 ttcgccatgg gaagaggtaa ggctccatga actgttaagt acaaaaccag tttcattgaa
    15241 tttttttaat gcattagata acaaaagaaa attaatttca acaaattatt gggaagtatt
    15301 tgctgcaaga aaaaaatgga tggaagaaaa gaagaatgcc gatcagcaga tccagtttgt
    15361 ccagctgaaa aaagaaaaac tatattatgg gttatacagc cgttaacagc aagcgaggaa
    15421 gaccatgaca ttaaaagaag aaatcataca agaaagcaaa cgattaggaa ttgacaaaat
    15481 cgggttcacc acggcggaac cttttgattc attaaaagaa tcgttggaag aacaaaaagc
    15541 agctggccat acttctggct ttgaacatcc aaatatcgat gagcgtcttt atccagaaat
    15601 gacatttgat cagccgaaat cgatcatttc cattgcactt gcgtatccta ctaggatcca
    15661 tgaagaagtg cctagggatg aaaaacgcgg gcagtttgct cgtgcttctt gggggatcga
    15721 ttaccatgat atactcagag accgtttgaa ccgattgatc gaattcatcc aaacaagagc
    15781 agaaaaatgg caaaaagaag aggaatggcg tctagctccg caagtagata ctggagaact
    15841 gatcgatgtc gctgtagcac agcgagctgg tttaggattt attggacgaa acggattgtt
    15901 gatcactgaa gagttcggtt catttgtcta tcttggtgag atcgttacta atattgtatt
    15961 tgagcctgat aaaccaggag aatttggttg cggtgactgt atgcgctgcg tgactgcttg
    16021 tccgacaaaa gcattgcttg gtgacggaag aatgaatgcc cagcgatgtt tgtcttatca
    16081 aactcagaca aaagggatga tgccggaaga atatcgtaaa aaaatgcgaa atgtcattta
    16141 tggatgtgat atctgccagt tagtctgccc ttacaatcaa ggaaaagatt ttcattttca
    16201 tgaagagatg gaaccaaaag tagacgaaat atatccaaaa ttaaagccaa tgctttcgat
    16261 gtctaataaa gacttcaaac agcaatttgg gcatttagct ggttcatggc gtgggaagaa
    16321 acctttgcag agaaacgcat tgattgcttt agctaatcta ggtgatcgaa cggcattgcc
    16381 tgagatttca gaatgtttaa cagatgtccg ccctgtcatc agaggaacag cagcttgggc
    16441 tattggacgt ctaggaacaa aagaaccgga aaaatggctt gatgtattag atgaaatatt
    16501 agctaaagaa acagaagaag tcgttataaa cgaattggaa catgcccaga aattattgcg
    16561 gagaa
//
LOCUS       DACEOE010000048        16528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEOE010000048 DACEOE010000000
VERSION     DACEOE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.050"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(142..417)
                     /locus_tag="IW774_001299"
     CDS             complement(142..417)
                     /locus_tag="IW774_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1153653.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(660..1970)
                     /locus_tag="IW774_001300"
     CDS             complement(660..1970)
                     /locus_tag="IW774_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1153654.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IW774_001301"
     CDS             complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IW774_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1153655.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3516..4196)
                     /locus_tag="IW774_001302"
     CDS             complement(3516..4196)
                     /locus_tag="IW774_001302"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1153656.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4245..4880)
                     /locus_tag="IW774_001303"
     CDS             complement(4245..4880)
                     /locus_tag="IW774_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1153657.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAKRAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4946..6349)
                     /locus_tag="IW774_001304"
     CDS             complement(4946..6349)
                     /locus_tag="IW774_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1153658.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6336..7367)
                     /locus_tag="IW774_001305"
     CDS             complement(6336..7367)
                     /locus_tag="IW774_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153659.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7398..7619
                     /locus_tag="IW774_001306"
     CDS             7398..7619
                     /locus_tag="IW774_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1153660.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7784..8521)
                     /locus_tag="IW774_001307"
     CDS             complement(7784..8521)
                     /locus_tag="IW774_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1153661.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8674..10014)
                     /locus_tag="IW774_001308"
     CDS             complement(8674..10014)
                     /locus_tag="IW774_001308"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1153662.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10251..11774)
                     /locus_tag="IW774_001309"
     CDS             complement(10251..11774)
                     /locus_tag="IW774_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1153663.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12152..12763)
                     /locus_tag="IW774_001310"
     CDS             complement(12152..12763)
                     /locus_tag="IW774_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1153664.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12886..13011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13093..13809)
                     /locus_tag="IW774_001311"
     CDS             complement(13093..13809)
                     /locus_tag="IW774_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1153665.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW774_001312"
     CDS             complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW774_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1153666.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14368..15165)
                     /locus_tag="IW774_001313"
     CDS             complement(14368..15165)
                     /locus_tag="IW774_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1153667.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMVIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15135..15506)
                     /locus_tag="IW774_001314"
     CDS             complement(15135..15506)
                     /locus_tag="IW774_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1153668.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW774_001315"
     CDS             complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW774_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1153669.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4646 a         3576 c         2797 g         5509 t
ORIGIN      
        1 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
       61 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
      121 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
      181 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
      241 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
      301 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
      361 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
      421 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
      481 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
      541 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
      601 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
      661 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
      721 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
      781 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
      841 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
      901 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
      961 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
     1021 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
     1081 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
     1141 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
     1201 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
     1261 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
     1321 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
     1381 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
     1441 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
     1501 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
     1561 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
     1621 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
     1681 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
     1741 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
     1801 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
     1861 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
     1921 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
     1981 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
     2041 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
     2101 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
     2161 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
     2221 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
     2281 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
     2341 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
     2401 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
     2461 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
     2521 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
     2581 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
     2641 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
     2701 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
     2761 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
     2821 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
     2881 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
     2941 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
     3001 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
     3061 aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt
     3121 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
     3181 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
     3241 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
     3301 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
     3361 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
     3421 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
     3481 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
     3541 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
     3601 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
     3661 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
     3721 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
     3781 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
     3841 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
     3901 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
     3961 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
     4021 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
     4081 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
     4141 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
     4201 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
     4261 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
     4321 tcagtggtga tccctgcacg cttagccact tgttttggac cttcgcctgc ttgaacagtc
     4381 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
     4441 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
     4501 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
     4561 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
     4621 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
     4681 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
     4741 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
     4801 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
     4861 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
     4921 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
     4981 aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
     5041 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
     5101 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
     5161 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
     5221 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
     5281 ttgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
     5341 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
     5401 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
     5461 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
     5521 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
     5581 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
     5641 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
     5701 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
     5761 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
     5821 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
     5881 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
     5941 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
     6001 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
     6061 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
     6121 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
     6181 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
     6241 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
     6301 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
     6361 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
     6421 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
     6481 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
     6541 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
     6601 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
     6661 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
     6721 aaaaggtgga tacatctgct ttgttgcaga taaacctctg actcatcatt gttttttgcc
     6781 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
     6841 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
     6901 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
     6961 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
     7021 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
     7081 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
     7141 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
     7201 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
     7261 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
     7321 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
     7381 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
     7441 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
     7501 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
     7561 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
     7621 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
     7681 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
     7741 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
     7801 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
     7861 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
     7921 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
     7981 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
     8041 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
     8101 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
     8161 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
     8221 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
     8281 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
     8341 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
     8401 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
     8461 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
     8521 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
     8581 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
     8641 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
     8701 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
     8761 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
     8821 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
     8881 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
     8941 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
     9001 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
     9061 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
     9121 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
     9181 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
     9241 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
     9301 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
     9361 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
     9421 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
     9481 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
     9541 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
     9601 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
     9661 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
     9721 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
     9781 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
     9841 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
     9901 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
     9961 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    10021 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    10081 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    10141 agaaaaaaca ctcactgaat ggagctattg acttgtccac gtgcttatcg atagccgttt
    10201 atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt
    10261 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    10321 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    10381 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    10441 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    10501 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    10561 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    10621 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    10681 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    10741 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    10801 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    10861 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    10921 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    10981 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    11041 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    11101 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    11161 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    11221 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    11281 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    11341 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    11401 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    11461 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    11521 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    11581 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    11641 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    11701 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    11761 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    11821 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    11881 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    11941 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    12001 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    12061 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    12121 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    12181 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    12241 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    12301 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    12361 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    12421 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    12481 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    12541 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    12601 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    12661 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    12721 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    12781 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    12841 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    12901 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    12961 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    13021 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    13081 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    13141 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    13201 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    13261 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    13321 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    13381 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    13441 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    13501 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    13561 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    13621 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    13681 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    13741 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    13801 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    13861 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    13921 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    13981 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    14041 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    14101 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    14161 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    14221 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    14281 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    14341 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    14401 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    14461 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    14521 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    14581 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    14641 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    14701 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    14761 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    14821 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    14881 gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattacca tcaatgtagc
    14941 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    15001 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    15061 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    15121 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    15181 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    15241 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    15301 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    15361 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    15421 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    15481 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    15541 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    15601 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    15661 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    15721 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    15781 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    15841 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    15901 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    15961 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    16021 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    16081 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    16141 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    16201 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    16261 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    16321 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    16381 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    16441 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    16501 tagacactca gacggactgg tttctttt
//
LOCUS       DACEOE010000049        16491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEOE010000049 DACEOE010000000
VERSION     DACEOE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.051"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..2706)
                     /locus_tag="IW774_001316"
     CDS             complement(1..2706)
                     /locus_tag="IW774_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1153670.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3012..3959)
                     /locus_tag="IW774_001317"
     CDS             complement(3012..3959)
                     /locus_tag="IW774_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1153671.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4149..4418
                     /gene="rpsN"
                     /locus_tag="IW774_001318"
     CDS             4149..4418
                     /gene="rpsN"
                     /locus_tag="IW774_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1153672.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4594..4956)
                     /locus_tag="IW774_001319"
     CDS             complement(4594..4956)
                     /locus_tag="IW774_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1153673.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(4967..6088)
                     /locus_tag="IW774_001320"
     CDS             complement(4967..6088)
                     /locus_tag="IW774_001320"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1153674.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6104..6454)
                     /locus_tag="IW774_001321"
     CDS             complement(6104..6454)
                     /locus_tag="IW774_001321"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1153675.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(6584..8095)
                     /locus_tag="IW774_001322"
     CDS             complement(6584..8095)
                     /locus_tag="IW774_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1153676.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8417..9769)
                     /locus_tag="IW774_001323"
     CDS             complement(8417..9769)
                     /locus_tag="IW774_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1153677.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(9922..10998)
                     /locus_tag="IW774_001324"
     CDS             complement(9922..10998)
                     /locus_tag="IW774_001324"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1153678.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11194..12939
                     /locus_tag="IW774_001325"
     CDS             11194..12939
                     /locus_tag="IW774_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1153679.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13219..13656)
                     /locus_tag="IW774_001326"
     CDS             complement(13219..13656)
                     /locus_tag="IW774_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1153680.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(13640..15013)
                     /locus_tag="IW774_001327"
     CDS             complement(13640..15013)
                     /locus_tag="IW774_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153681.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15335..15823)
                     /locus_tag="IW774_001328"
     CDS             complement(15335..15823)
                     /locus_tag="IW774_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1153682.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(15845..16447)
                     /locus_tag="IW774_001329"
     CDS             complement(15845..16447)
                     /locus_tag="IW774_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1153683.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4626 a         3532 c         3008 g         5325 t
ORIGIN      
        1 ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat
       61 gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca
      121 gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac
      181 cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg agacttggat
      241 cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt
      301 accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat
      361 caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat
      421 attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg
      481 agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc
      541 tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa
      601 cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac
      661 aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc
      721 gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc
      781 tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac
      841 ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg
      901 ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc
      961 attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt
     1021 cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt
     1081 ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa
     1141 gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg
     1201 gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc
     1261 attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc
     1321 ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg
     1381 ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg
     1441 caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc
     1501 aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc
     1561 ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat
     1621 tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga
     1681 ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc
     1741 tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc
     1801 ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc
     1861 ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt
     1921 caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg
     1981 gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact
     2041 ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga
     2101 taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat
     2161 ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg
     2221 cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc
     2281 atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc
     2341 ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc
     2401 ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga
     2461 ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc
     2521 actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata atgctttgat
     2581 gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat
     2641 cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga
     2701 ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac
     2761 ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct
     2821 cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga
     2881 aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa
     2941 agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat
     3001 tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca
     3061 actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa
     3121 acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga
     3181 tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga
     3241 tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt
     3301 tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc
     3361 agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt
     3421 tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg
     3481 atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg
     3541 atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct
     3601 gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact
     3661 tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt
     3721 gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg
     3781 aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc
     3841 acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca
     3901 tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg
     3961 gtcaccatct ccctttttct ttaagtttac gtttatgtct ttattttgac aaaaaaaatg
     4021 tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt
     4081 aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga
     4141 atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg
     4201 aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta
     4261 aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc
     4321 ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata
     4381 aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa
     4441 aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta
     4501 gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt
     4561 atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc
     4621 gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg
     4681 gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac
     4741 ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg
     4801 tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag
     4861 attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga
     4921 aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt
     4981 ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat
     5041 ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg
     5101 ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa
     5161 tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc
     5221 catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt
     5281 ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca
     5341 gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc
     5401 cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg
     5461 ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta
     5521 taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat
     5581 gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt
     5641 ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa
     5701 tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt
     5761 cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag
     5821 gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt
     5881 catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt
     5941 caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg
     6001 gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt
     6061 tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa
     6121 tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag
     6181 gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg
     6241 taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc
     6301 cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag
     6361 aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa
     6421 gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta
     6481 gaacactctc attctaccaa attataacca ttttgccaca aataagtcca caaataacaa
     6541 aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac
     6601 ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat
     6661 tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat
     6721 tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca
     6781 atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt
     6841 ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt
     6901 gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac
     6961 ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt
     7021 tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag
     7081 acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat
     7141 cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag
     7201 caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca
     7261 ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta
     7321 atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag
     7381 gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga
     7441 catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg
     7501 gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa
     7561 gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc
     7621 ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacagtt ccaagtttca
     7681 atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac
     7741 gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac
     7801 gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac
     7861 gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca
     7921 ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt
     7981 ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag
     8041 cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa
     8101 tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg
     8161 gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc
     8221 ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc
     8281 gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac
     8341 cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct
     8401 gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca aataagccca
     8461 tgctgttgct gccttttagc ataatactat ctgttgctgc aagattttgt ttgatagcat
     8521 caatcaattt attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt
     8581 tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct
     8641 caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca
     8701 gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta
     8761 ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat
     8821 ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc
     8881 gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg
     8941 ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg
     9001 tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg
     9061 ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca
     9121 caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc
     9181 cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt
     9241 ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca
     9301 aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat
     9361 tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta
     9421 gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt
     9481 tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa
     9541 ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt
     9601 ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata
     9661 aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag
     9721 agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc
     9781 ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt
     9841 ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc
     9901 cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta
     9961 tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt
    10021 ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt
    10081 caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc
    10141 taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac
    10201 ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa
    10261 tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac
    10321 agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg
    10381 ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac
    10441 actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg
    10501 atcaaatact tttttaggat tttctttcca ttgattctta agtactggta cataaggaac
    10561 ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc
    10621 actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat
    10681 cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt
    10741 gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc
    10801 ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgac
    10861 ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc
    10921 atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata
    10981 tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc
    11041 tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc
    11101 ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat
    11161 aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc
    11221 ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa
    11281 aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata
    11341 ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa
    11401 acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca
    11461 gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct
    11521 gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag
    11581 atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg
    11641 atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa
    11701 gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa
    11761 caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct
    11821 ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta
    11881 atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa
    11941 caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt
    12001 ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag
    12061 acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt
    12121 cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt
    12181 ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg
    12241 ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa
    12301 ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat
    12361 gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt
    12421 catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca
    12481 ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct
    12541 tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga
    12601 ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta
    12661 ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa
    12721 ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca
    12781 ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt
    12841 tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc
    12901 gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga
    12961 atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa
    13021 ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag
    13081 ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt
    13141 ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc
    13201 ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg
    13261 atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc
    13321 actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga
    13381 gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt
    13441 gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc
    13501 gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac
    13561 actggtcacg ataaccacgt caagaccata ggattctcct aattttatga catcttcttt
    13621 tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat
    13681 gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa
    13741 gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga
    13801 ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga
    13861 tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag
    13921 ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac
    13981 taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac
    14041 tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc
    14101 cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt
    14161 tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta
    14221 gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct
    14281 ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat
    14341 caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga
    14401 atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat
    14461 ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat
    14521 aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga
    14581 gaaatagcca gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg
    14641 tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta
    14701 gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg
    14761 aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc
    14821 gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca
    14881 aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa
    14941 taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga
    15001 gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt
    15061 tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag
    15121 aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag cagacaagcc
    15181 cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa gatagaaatc
    15241 aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc ttctcgaccc
    15301 gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt ttcattttat
    15361 aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta atctcaaatc
    15421 cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc aaaaagatct
    15481 ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa gaagtcaatc
    15541 cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt ccatttatct
    15601 cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag taggacacac
    15661 cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc atgtaatcca
    15721 cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt atgactgcca
    15781 tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca cgtccttttt
    15841 tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg ctatacacac
    15901 ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa gataatttcg
    15961 tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa
    16021 ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact
    16081 tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca agcaaaaatc
    16141 cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat ccattgccaa
    16201 acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg atcacttgga
    16261 tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg ataaacgctt
    16321 gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc aacacaaaga
    16381 aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact gatccttttt
    16441 tagacatcta tattccccac atcttttttc atttattaaa actgcctcct t
//
LOCUS       DACEOE010000050        16459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEOE010000050 DACEOE010000000
VERSION     DACEOE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.052"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            345..3179
                     /locus_tag="IW774_001330"
     CDS             345..3179
                     /locus_tag="IW774_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1153684.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3370..3861
                     /locus_tag="IW774_001331"
     CDS             3370..3861
                     /locus_tag="IW774_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1153685.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3964..4953
                     /gene="manX"
                     /locus_tag="IW774_001332"
     CDS             3964..4953
                     /gene="manX"
                     /locus_tag="IW774_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1153686.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4986..5789
                     /locus_tag="IW774_001333"
     CDS             4986..5789
                     /locus_tag="IW774_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1153687.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5810..6724
                     /locus_tag="IW774_001334"
     CDS             5810..6724
                     /locus_tag="IW774_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1153688.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6807..7181
                     /locus_tag="IW774_001335"
     CDS             6807..7181
                     /locus_tag="IW774_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1153689.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7320..7565
                     /locus_tag="IW774_001336"
     CDS             7320..7565
                     /locus_tag="IW774_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1153690.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7737..8801
                     /locus_tag="IW774_001337"
     CDS             7737..8801
                     /locus_tag="IW774_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1153691.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9047..9472
                     /locus_tag="IW774_001338"
     CDS             9047..9472
                     /locus_tag="IW774_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1153692.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9465..9914
                     /gene="nusB"
                     /locus_tag="IW774_001339"
     CDS             9465..9914
                     /gene="nusB"
                     /locus_tag="IW774_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1153693.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10011..10865
                     /locus_tag="IW774_001340"
     CDS             10011..10865
                     /locus_tag="IW774_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1153694.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10866..12203
                     /locus_tag="IW774_001341"
     CDS             10866..12203
                     /locus_tag="IW774_001341"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1153695.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12208..12432
                     /locus_tag="IW774_001342"
     CDS             12208..12432
                     /locus_tag="IW774_001342"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1153696.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12434..13324
                     /locus_tag="IW774_001343"
     CDS             12434..13324
                     /locus_tag="IW774_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1153697.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13333..14151
                     /locus_tag="IW774_001344"
     CDS             13333..14151
                     /locus_tag="IW774_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1153698.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14258..14707
                     /locus_tag="IW774_001345"
     CDS             14258..14707
                     /locus_tag="IW774_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1153699.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14725..16407
                     /gene="recN"
                     /locus_tag="IW774_001346"
     CDS             14725..16407
                     /gene="recN"
                     /locus_tag="IW774_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1153700.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
BASE COUNT         5439 a         2731 c         3704 g         4585 t
ORIGIN      
        1 aataaaagtt gtagtggagg ctacgaagtt tactttgtct ttttacactt aatatttttt
       61 tgccaatttg gaatcaggat gttttgataa ctatcgtttt caattgatgg aagctatatt
      121 gtttgctatc agtgaaataa ttatatggta aacagtgttc tttttcttgt ttccattata
      181 aaaatcgtat cgggattttt cgttctttta aattgaacat ttctatctat ccgcgtgtta
      241 tcaacaaaac aaaatcaggt aaatagttga cgaaacagaa ataaatacat agtattatgt
      301 tttgaagcct aagtacaata gtctatagaa aaggagcgat accattggca aaacgcattg
      361 accgaattta tatgtatgtc aaagaaaaaa ccgctcattt aacaaaggca gaatatgatc
      421 aaggagtaac aacacaggaa attgctgaag tgttagggat tcagcgaaca aatagcagta
      481 aagatctgaa tcagctagtg cgcgaaggaa aactgataaa gacagacgga cgaccagtac
      541 gttacatata tcaaacactc gttacgcacg taaagccagt tacaaaacat gtagtcagtt
      601 acaaagaaac gccggtctct gaagaaaaaa atacactcca aatagatact aaggatatct
      661 ttgcaaaaat cattggagct aacggcagca tgaaaaattc agtggaacag gcaaaagctg
      721 cgattttgta tccgccaaga ggattaaatt gtttgattac aggacctacc ggatcaggaa
      781 agacttattt tgcacacgcg atgtatcatt ttgcaaaagc gcatcatgta atagatgagg
      841 aaacggaact gattgttttc aattgtgctg attatgccaa taaccctgag cttttgatga
      901 gtcatttatt tgggtacgca aaaggtgctt ttacaggcgc agatgaagaa aaaatgggga
      961 ttatcgatca agcggatggc ggtatgctgt ttttggatga gattcaccgg ttgccgcctg
     1021 aaggtcaaga aatgatcttt tactttatgg atcatggaac ttacagtcgt cttggggaaa
     1081 caacgaaatc ccatgaagca aatgtacgga tcgtaggtgc gaccacagaa gacccaggtt
     1141 cttctctatt agaaactttt gttcggcgta tcccaatcaa tatcaaactt ccggcttttg
     1201 aaaaacgtcc tgcaaatgag aaagtcgatt tagtaaaaat catgattgcc cacgaagcaa
     1261 accgcacgca gcgaaagatt tctttgacag aagatgtagc aaaagcattg atcggcagtg
     1321 tgacctatgg aaatatagga caattgaagt caaatatcca gttagtatgt gcacgaggat
     1381 ttctaaatca tatgcaatca cccgaaatct cgatcacaat cgatgatttg acagaaggga
     1441 ttcgtagcgg attgattcaa ttagcaagca accgcagtgc aatggcagag ctttctaaaa
     1501 tattggagcc aaagataact gtagcaccaa atgatacgat catgaaaatc caatcggact
     1561 cctatgagtt accttataat ctatatgata tcatcggaga taaagcagct ttactgaagt
     1621 cggatggttt ggatcaagaa gcaatcaatc atttcatttc tacagatatc aacatccatc
     1681 tgaagtcttt ctataaagac catggttttt cttttaatgc agacagcaaa ttagcagaat
     1741 ttgtagatcc gaaaatcata gaagtgacca atcagattta tgtaatggtc aaaagatctt
     1801 taccatatga gtttcagcaa aactttattt atgctatgag cttgcatatc agttcttttt
     1861 tgaaaaaaat caacttagga gaagaacggc atacgaatga taacatccgt gagatggctt
     1921 ttgattttcc agatgaatat gctgttgcga aagaagtgcg tagttatatc gaaacgtatt
     1981 ttcaagtgga gattccagaa agtgaagact attacttgac cgttctactc gtctcgttac
     2041 gggcaaatca agcaagcggc agaatcggtg tagtcgttgc agctcacgga aacagcacag
     2101 caagcagtat ggtccaagtt gtccaacagt tgttagatgc agagaacgta cgagcagtag
     2161 acatgccttt ggatatggat ccgaaaacag cattggaccg aattgaacgc aatgtacaag
     2221 aagtagatga aggaagcggg gtcatactct tagtagatat ggggtccctt gcgtcgttta
     2281 attcgcaaat tcaaagagac actggaattc cagtacgtac tgttgatatg gtaacaactt
     2341 ctctagtttt ggaaactgta cgaaaggtct cagtattagg gacagatcta gatatgttgc
     2401 atgaatcatt gaaaaatttt cgaggttacg cagaaatatc ttctgtagaa tctgttcagg
     2461 aagcaaaacc tattcataag aaaaaagctg ttttggctat ctgcgcatct ggcgaaggta
     2521 cagcacaaag aatcaaagaa ttgatcgaac gagcggtaaa caagcgtcaa gaaacagaat
     2581 tagaagttct agctttgtcg attgtggaag tgaaagaaga actgccagtt atccaaaaag
     2641 aatatcagat catagctact actgggatta ctgatttgaa tatccaagcc ccatttattc
     2701 ctttagaacg ttttattgat caaaatatcg aagtcatttt ggatcagttg ctgatggaat
     2761 cagaattgga ggaaactgaa ttcatctccc tagatgaaga gtcagctaaa aatacgtgtg
     2821 ttgagtttat ctctgataac ttcatcttta ttaatggtag taagttaatc gatccaatgt
     2881 ggcaattttc tacacaaatc tctcagacga cgggaatcgg cgatgaagaa tatggattca
     2941 aaatcaacct agtgatgcac acagcaggaa tgatcgaacg tattattcgg aatgaaccat
     3001 tgactgtgga agaaaacgaa ttgacaaata caacgaatga tcctctttat agccaattgg
     3061 cagcctcagt tgttctgttg gaagatcaga taaaagtaaa agtgccgata gaagaaatgt
     3121 attatctgct acgtctagtc cataatcagt tagataaaaa agaatacact gtgccatgaa
     3181 tacacaaaaa cgatacactt ttatagtgta tcgtttttgt gtattggtta agaatccttt
     3241 aaattcaatt agtgtatcgt aaaaaagttg gcacgctttt tgcttatagt agtgtgtgct
     3301 aggaggtggt tgttcacaca gaaatgatag gggtttctgt gaagtagtga ggaagaagga
     3361 gttttgcaga tggatattcg attggcacgt atagatgatc gtttaatcca cgggcaagtc
     3421 gcaacagttt ggtcgaagca aacaggtgtt gaacgtatca ttgtgatcag cgatgtagtc
     3481 gctcaagata aattgagaaa gtttctacta aaagaagctg caccgccggg gatcacagca
     3541 aatgtaataa ccgtgcagaa aatgattcaa atctatcata gtggattatt atcacgagca
     3601 aaagtgatgc ttttattcac caatccccaa gatgttgaag cggttgtccg ctgcggtgta
     3661 cagttgaagt cgttgaatat cggcggtatg agttttactg aaggcaaatc aatgattacc
     3721 aattttgttt cagtaaatca acaagatatt gattcctttc gttacttgaa cagtcaagaa
     3781 gtggagttag agattcgaaa agtacctgct gacaggaaaa tccttttgat ggaactacta
     3841 gcgaaaatca aaaaggtgta gcaagaatgg ttgttggaaa aggtcaaata attctgttgt
     3901 aatggttagc aattattaat atttttgaat ttgttacaac aaaagtaaat aggaggtata
     3961 aatatggtag ggattatcct agcaagtcat ggacaatttg ctgaaggaat cttacaatcc
     4021 ggttcaatga tctttggcga acaagaaaac gtcaaagccg tgattttgaa accaagtgaa
     4081 ggtccagatg acttgagagc caaattggaa gaagcggttg cttcttttga taatcaagac
     4141 gaagtgttat tcttagttga tttatggggc ggaacaccgt tcaatcaatc aaacacatta
     4201 ttcgaagagc acaaagataa atgggcaatc gtcagcggtt tgaatttacc aatgttgatt
     4261 gaagcctacg catcacgctt ttcaatggaa tctgctcatg aaattgcagc acacatcatt
     4321 gaaacagcaa aagacggtgt aaaagtaaaa ccggaagaat tagaaccggc agaagcaccg
     4381 aaagcagcag ttgaagacgc acaacctaaa ggagcacttc cagaaggaac tgtggtcgga
     4441 gacggaaaaa tcaaatatgt attagcacgt gttgactcac gtctgttaca tggacaagtt
     4501 gcgacagctt ggacaaaagc agtgcaacca aaccgtatta tcgttgtttc agatgcagta
     4561 tcaaaggatg atctgcgtaa gagactaatc gaacaagctg ctcctccagg tgtaaaagca
     4621 aatgttattc ctattagcaa aatgatcgaa gtagcaaaag accctcgttt tggaaatacc
     4681 aaagcattgc ttttatttga aaatcctgaa gacgtattga cagctgttga aggtggcgta
     4741 gacatcaaag aattaaatgt tgggtcaatg gcgcattcgg tcggtaaagt cgttgtaagt
     4801 aaagtattgt ctatgggaca agaagacgtt gaagcgtttg agaagcttga acaaaaaggt
     4861 gtgaaattcg atgtacgtaa agtacctaat gattcacgcg ataacatgga tgatattttg
     4921 aaaaaagcaa aagctgaatt agcaaaagcg taataaataa tattaaaatt tataggaggc
     4981 ttatcatgtc tattatttca ataattttag tcgtattagt tgcctttcta gctggtatgg
     5041 aaggtattct tgatgaattt caatttcatc aaccactagt ggcatgtaca ttgatcggtt
     5101 tagttaccgg taacctcgaa gctggtatcg tacttggcgg tacgcttcag atgatcgctt
     5161 taggttgggc aaatatcggt gccgctgtag caccagatgc tgcattagca tcagtagcat
     5221 cagcaattat tttagtttta ggtggacaag gtgttaaagg tgttccttct gcgatcgcga
     5281 ttgccgttcc tttggctgtt gcagggttgt tcttaacaat gatcgtacgt acaatcgctg
     5341 ttccaatcgt tcatttgatg gatgcggctg ctgaagaagg aaacatccgt aaggttgaaa
     5401 tgtggcacat cattgctgta tgtttacaag gggttcgtat cgcggtccca gcagcagcgt
     5461 tgctatttat cccagcacaa actgtgcaat cattccttga atcaatgcca gcatggttaa
     5521 cagatggtat ggctatcggt gggggaatgg tcgtagctgt aggttacgct ttggtaatca
     5581 acatgatggc tacaaaagaa gtatggccat tcttcgttat tggtttcgtt gtagctgcaa
     5641 tttctcaatt aacattgatt gctctaggtg ctttaggtgt tgcattagct cttatttact
     5701 tgaacctttc taaaatgggt ggttcatcaa ataatggagg cggaagcaac tctggtgacc
     5761 ctttaggcga cattttgaat gactattaat cttgaaggag gaaaataaaa tggcagaaga
     5821 aaaaatcaaa ttatcaaaaa aagatcgttt agctgttgcg tggcgctcta ccttcattca
     5881 aggttcatgg aactacgaac gtatgcaaaa cggtggctgg gcattttcta tgattccagc
     5941 aatcaaaaaa ttatacaaaa caaaagaaga acgttctgca gcattaaaac gtcacttgga
     6001 attctttaat acacacccat acattgcttc tccaatttta ggggttacat tggctctgga
     6061 agaagaacgt gcgaatggcg cgcctgttga tgatgtagcg attcaagggg ttaaagtagg
     6121 tatgatgggt cctttagctg gtgttggtga cccagtgttc tggtttacag tacgtccaat
     6181 gctaggtgca ctaggtgctt cattagctat gggaggaaat atccttggac caatcatctt
     6241 cttcttaggt tggaacatta ttcgttgggc attcatgtgg tatacacaag aatttggcta
     6301 caaagctggt tctaaaatta ctgatgacct ttctggcgga ttgttacaag acgtaacaaa
     6361 aggtgcttca attttaggga tgttcgtatt agctgccctt gtacaaagat gggtatcaat
     6421 caaattctta ccagttgtat caactgtaaa attagataaa ggtgcttaca tcgaatggga
     6481 taaattgcct tcaggtggtg aaggaatcaa gagtgcattt gaacaagtta ataatggctt
     6541 agccctttct cctgaaaagg taacaacatt acaagacaac ttagaccaat tgattcctgg
     6601 tttagctgca ttaggattaa cattgttatg tatgtggttg cttaagaaaa aggtttctcc
     6661 aatcgttatc atccttggtt tattcgtagt cggtgttgtt ggacacgtca tcggactttt
     6721 ataatccata aaaatatagc tcgggcgttg tcccgagctt tttttatata gtatactgtt
     6781 cataaacaaa tatctggagg gagaaaatgg ttcaatcaat caatacaaaa gtagacttgg
     6841 taattgatgc tacttcgttt acgggtctga cagattacgg gcaaatcatg ataggtgata
     6901 atggttttga gttttaccat gcgcgagata gacgcaaata tatccaaatt ccatgggaag
     6961 aagtggatta tgtactagca tcagtcatgc taaagggaaa atggattcct cgatatgcca
     7021 ttcaaacgaa aaaaaatggt acatacacat tttcttccaa agaacctaaa aaagtgcttc
     7081 gtgcgatcag agaatatgta gatgaagata gaatggtaca atcattgagt ttctttgatg
     7141 tagtgaaacg tggtattcgt agtatgttta aacgcaagta aaaaataaag aaaacttcta
     7201 agaggaggtt ttctttattt ttttactttg cagaagaaaa tatgaaatag caaaaccaat
     7261 tatttgtccc ctttaaatcc catatgctaa aattgattag caaagaagga ggttatatta
     7321 tgagttggtt atggacattg attgttggag ccgtgatcgg agcaattgca ggtgcgatca
     7381 ccagtaaagg aaattcgatg ggctgtgtaa cgaatatcat tgctggacta gtcggttcaa
     7441 gtattggaca agcgattttc ggcgaatggg gaccaaaagc tgcaggtatg gcacttattc
     7501 catctatttt aggcgcagtg attttggtag cagttgtctc atttttcttt ggaaaacgat
     7561 cataagtttt tacaaaatag taaactaaaa ggagttgcag cgagactgtg actccttttt
     7621 ggctttattt atttaaaata aaaagaagtt ttccggaaat ttttatttaa ctgtcgtgta
     7681 cgaagaaaag tttgctataa tggaaaaaag cgtgcaaaga aagagggagt ttcattatga
     7741 tgctacgtgt agaaaaattg cgcaaaaaaa tgcaagaaga aaatttggat tcatttttgg
     7801 ttaccagtcc gtacaatctt cgttacttaa cgaactttac aggtacgaca ggattagctg
     7861 ttattacttt ggaaaaagca ttttttatta ctgattttcg ttataccgag caggcggcag
     7921 cgcaggctca agggtttgaa attattaaaa atgtcggacc tatttttgat gaggtagcaa
     7981 acttagtacg caaagaagaa ctgcactcat tagcttttga ggaaacaaca gtctcatttt
     8041 tagaatattc ggttttagaa gaaatcatcg atgcggaatt agtacctgtt tcacagatga
     8101 tcgaagaact cagagaagtg aaagatgaag aagaaatcgc tattatcgaa aaagcatgtc
     8161 atattgcgga tatggcatat gatcacatcc tgaaaatgat ccaaccgggc atgactgaga
     8221 ttgaagtcgc aaatcaacta gacttttata tgcgttctct tggcgcttca ggtgtttctt
     8281 ttgatacgat cgttgctagc ggattgcgtt cggctatgcc gcatggtgta gcaagcaaaa
     8341 aaatcatcga acaaggtgac ttgatcacat tggactttgg ttgctattac gaagggtacg
     8401 tttccgatat gacacgtaca ttcgctattg gtgatcctgg ggaaaaatta aaagaaattt
     8461 atcaaatagt cttagatgct caattagcag ttattgatgc tgcaaaacca ggaatgactg
     8521 gtgtccaatt agatgcagtt gctagagatt atatttcaaa acatggttac ggggaagcat
     8581 ttggacattc tacaggacat gggatcggtt tagagatcca cgaaggacca aacgtttctt
     8641 ctcgtgcaga aaagcaattt gtaccaggaa atgtgatcac tgatgaacca gggatctact
     8701 taccaggaat cggtggagta cgtattgagg atgacttgtt gatcacagaa gatggaaatc
     8761 gtgtgttgac tcgttcacca aaagaattga tcatcttata gtttcatcaa tagaagaagt
     8821 aagcgtaaaa atcagtgttc acagactttg ttatagaaaa tcactcgatt taatgataca
     8881 ctatagaaga acttttaagc tgtaagagat aataaagttg ggtgttttct ccaattcata
     8941 gcagtttgca gctgatcggc ggcagttttc ttaccacgtg atcactatgc cgcatcatag
     9001 agaattcgga tgtatctcgt gcttaggcaa agggaggaat aaataaatgg cagatgaaaa
     9061 aaatttgatt ttaaacgcaa accaagattt aggagagatc gtgatcgctc ctgaagtcat
     9121 cgaagtaatt atcgggattg cagcttctaa agtagaaggc gtatacggaa tgcgcggaac
     9181 ttttgccagc aatgtcactg aattattagg acgtgcagct cacggaaaag gtgtttactt
     9241 agcaaacgag gaagatggct tgaaagtaga tctttactgt tatctagaat atggtgtttc
     9301 agttcctaaa gtagcaatgg aaatgcagga acgggtcagc cagcaagtcc ttttcatgac
     9361 ggatatcgat ttatctgaag tcaatatcca tgtagtagct gttgttcctg aaaaattaga
     9421 acaaccaaca atcgaagaac tttttgaaga cgaagaggaa gagaatgaat aaagaattat
     9481 caagacacga gattcgggaa atggcattgc aggctttatt tccattagac ttcaatgcag
     9541 acctaacaaa agaagatgcg attttcaacg caatcgaatt agaccaccgc gacatgatca
     9601 acgaagatga atcagagttt gttcctgttt atcttgatac gctagttggc ggtgtctgtg
     9661 caaaaaaaga tgaattggat aaagtgattg aaaaacattt aaaaaacaat tggcacatca
     9721 atcggatctc aaaaatggat ttggtgatct tgcgtatcgc gatttttgaa atgacttatg
     9781 tgacagatgt accagcggct gttgctttaa atgaagcaat cgagttggca aaaacattta
     9841 gtgatgaccg ttcacgtaaa ttcgtcaacg gtgttttatc caatgtattg aaagaattag
     9901 aagctggggc gtaagtccta gcttttttct atgaatcatc ataaacctgt tataaatcgg
     9961 gaaaattcta tggtagaatg aaaacacaaa aaatatctgg gagtggttag atggcagaat
    10021 tgattaacgg aaaagaatta gctgaaaaaa tgcaggcaga gattgctgaa aaaatccaaa
    10081 aattgaaaga taatggtatc catcccggat tagtcgtttt attggtaggg gaaaatccag
    10141 ccagccaaat ctatgtaaga aataaagaac gtgtagcaaa agaaatcgga atccattcat
    10201 tggtagagcg ttatcctgag acgatttctg aagacgaact acttgcagaa attgaaaaat
    10261 acaatcaaga tcctagtttt catggtatat tggtccaatt accattgcca aagcatatag
    10321 atgaagaaaa gatcctgtta gctatccgcc ctgaaaaaga cgtagacggt tttcatccga
    10381 tgaatttagg cagactcctt gcgggtaaac cggacaagat tccttgtact ccatacggca
    10441 tcatgaaaat gtttgaagct tattctatcg atccggcggg taaacgagca gtagttatcg
    10501 gacgaagcaa tatcgtcggt aaaccgatgg ctcagctact cttgatggca gatgcaactg
    10561 ttactgttgc tcattctaaa accgaaaatc tggctgaact ggcacaagaa gcagatattt
    10621 tagtcgtagc aataggacgt ggacattttg tcacaaaaga attcgtaaaa ccaggtgctg
    10681 tagtgataga tgtagggatg aatcgtgatg ctaacggtaa gctgatcggt gatgtgaaat
    10741 ttgatgaagt ggagcctatt gcaggctgga tcactcctgt gccaaagggt gtagggccaa
    10801 tgactatcac gatgctgatg tatcaaaccg taaaatctgc cgaagctttt ggagcaggtg
    10861 aatagatggc acaagaatat ttaactgtca ctacattaac gaagtatttg aaaagaaaat
    10921 ttgatgccga tccttattta gaacgcgtat atttaacagg cgaaatatcg aatttccgtt
    10981 tacgtgccaa tgcccatcaa tatttcagct taaaagatga ccatgcaaaa atatctgcta
    11041 ttatgttcaa aggagcattc caaaaattac gattccagcc aaaagaagga atgaaagtcc
    11101 ttgtcgttgg gcgtatttca ttatatgaag cgggaggttc ttatcaaatc tatgtagagc
    11161 acatggagcc tgatggaata ggtgctttat atcaagaatt ggaagaacga aaagaaaagc
    11221 tgatgaagga aggccttttt caagggccta aaaaaatgct gccacgtttc ccaaagcgaa
    11281 tcgcggtcct taccagtcca agcggagcgg ttatccgcga tatcatcacg acggtgaaaa
    11341 gaagatatcc aattgtccaa ttggttttgt tcccaacagt tgtacaagga aaccaagcag
    11401 cggacaatgt tgtcaggaat atccagagag tagaagagat gggcaatttc gatacaatga
    11461 tcatcggtcg tgggggtggt tcgattgagg atttatggcc gtttaatgaa gagcgggttg
    11521 ctcgggcgat ttatcaagct aatacgccgg tgatttcatc tgtaggacat gaaacagata
    11581 ccacgatcgc tgatttagta gcagatgttc gggctgccac tccaacagca gctgcagaat
    11641 tagctgttcc tgttttgtca gaagagttga tgcgcattca agaacggcaa gctagattgc
    11701 agcaggcgtt tgtacgccag atccagcgca aacaagaacg gttcgagcgt gctcgacgtt
    11761 cctatgtatt tcggcagcct gaaagattgt atgaaggtca gaccatcaaa ttagaccagt
    11821 tgaaacagcg attatatcaa gctacacagc aaatttatca tgcgaaagaa aaacaaacga
    11881 tcgcattaag ccaccaattg gaacaagtgg ctccgcttta tcgagttaaa gcagctcgcc
    11941 aagaaactgc ttatctagaa aaacgcttag aagagaaaat ccaacaatat atgaaacaac
    12001 agcagcagcg ttttgctcaa gctgttcaat ctcttgattt gttgagtcca ttgaaaatca
    12061 tgggaagagg ctacagctat acgacgttaa atgatcaagt agtaaaaagt gtcaccgaga
    12121 ttcatcaagg ggatttgctg acgatccatt atcaagatgg aatcgttaaa ggacaagtac
    12181 aagagataga caaaggagaa taaaaatatg gaaaatccaa cttttgaaga gtcactgcaa
    12241 gaattagaaa aaattgtcat gcagttagaa caaggagacg ttccattaga atcagctcta
    12301 gattcgttta aacgaggaat ggaattaagc aaacattgcc aagatacatt agctaaagca
    12361 gaagagacct tgacgaaaat gatgacagaa tcaaatgaag aagttgattt tgacggaaac
    12421 gaggaaagct gagatgagat taactgactt ttcagctatt catctacctg ctgtagaaaa
    12481 agaaatcttg tcttttttag atgagcatac aacggataga ggactctatg atgcgatgag
    12541 ttattcagtg aaagcaggcg ggaaaagaat acgtccctta ttacttttaa cggccgttgc
    12601 ttcctttgat gagcctattg atgttcccgt ctatcaagta gcagctgctt tagaaatggt
    12661 gcatacgtat tccttgatcc acgatgattt gcctgcgatg gacaatgatg atcttcgtag
    12721 aggaaaacct accaatcata aagtattcgg cgaagcatta gctatattgg ccggagatgg
    12781 tttgcttaca ggagcattcc agctaatcag tatggcacac ttaggtaatt ctcctaagct
    12841 tctgctgctt cagcaattag ctgtttgtgc gggatcacaa ggtatggttg ctgggcaagc
    12901 tgcagatatc gagggcgaat cgaaaaagct ttctttagaa gaattagcat ttattcatga
    12961 aagaaagaca gggcatttga ttcgttatgc cttgctggct ggtggcatat tagcgaagca
    13021 acctgaagaa atcttattgc tacttcaacg attagccgaa catttaggtt tagcttttca
    13081 gataagagat gatctactcg atgtgattgg tacaacgaaa acattaggga aaacagctgg
    13141 taaagatgaa cgtatggaaa aaaatactta cccgcgttta ttaggcctag aaaagacacg
    13201 tgaggcgttg gagattgagc tgttatccgc caacaagatc atagacaaat tagaagagga
    13261 cgttttttct tttgatggcg ggctgatgcg ccagatgatc aagcaatttg atgtggagaa
    13321 atgagggaaa gaatggaaaa agaacgcgta gatgtattag cggtcaaaca aggattattt
    13381 gaaacaagag aacaagccaa acgttcagtg atggctggtt tgatttacaa cgaaaaaaac
    13441 gagcggtttg ataaaccggg agaaaaaatt ccagtgagta gtgaattaag agtcaaaggt
    13501 aaaaaacttc cttatgtttc tcgaggggga ttaaaattag aaaaagcact caaagcattt
    13561 gatctatctg ttgaaggaaa aacattgtta gatattggtg catccactgg cggatttact
    13621 gatgcagctt tgcaaaatgg cgcaaaaatg agttatgctt tagacgttgg ctacaatcag
    13681 ctagcttgga aaataagaca agatcctaga gtcgtcgtaa tggaaagagt gaattttcgt
    13741 tatgcaaaac ccgaagactt taccttagga attccagaag ttgctgttat cgatgtttct
    13801 tttatttcac taaaactgat gctgcctcca ttacatgcta ttttaaaaga agagggagaa
    13861 gtgattgctt taatcaaacc ccaattcgaa gcaggcagag aagctgtagg gaaaaacgga
    13921 attgttcgtg accctcagac ccatcaaaaa gttttagaag acattttgtc atttgcggca
    13981 caaggtggat atgatgtgct ggaattaagt tattctccga tcacgggggg agaaggaaat
    14041 atcgagttct tggctcactt gaggaaagta cccgaaagcg gaacaatcaa ttcagctatt
    14101 aatatggcag aagtggtttc aaatgcacac gaacagtttg accataaata aattgtaact
    14161 gtgaaaatca agtgagaagt atgcggattc ttctttatta taccaaggaa ataagctatc
    14221 attgattaag aagaatatgc taaggagcga agacgtaatg agaaaaaaag atcggcatcg
    14281 tttgattact cgattgttga gtgagcaaga tatccgaaag caggaagaat ttgttgaaat
    14341 cctgaaaagc aaaggaatat ctgttactca agctacgatt tccagagata taaaggaact
    14401 gaaactgatc aaggtaccgg cttcagatgg cggctaccgt tatagcatac cagctgaaac
    14461 aaacgaagat gtaaatacta aattggaaaa actattaaaa gacgctttcg tggcagttga
    14521 ccaaatggaa aaatttgtta ttttgaaaac attgccggga aatgcttcgg cagcagcaaa
    14581 tttgattgat aaacgttaca agaaagaatt attttcgatt atcaatgatg acgataatgt
    14641 tttgatgatt acacgaactg aagaccaggc agttttattg aagaaagact tcctgcatta
    14701 cctttaagtt gaggagcgaa tgtgatgcta caagaaatca gtattactaa ctttgcgatt
    14761 attccggaat tgcggctttc ttttcacgaa ggaatgaccg cgctgaccgg agagactggt
    14821 gcgggtaaat ccattattat cgatgcttta ggtctgttag caggaggaag aggttcaagc
    14881 gattatatac gtcaaggagc agaaaaatgt gtgcttgaag ggttgtttga actaccaaaa
    14941 caagaaggat tctctgaact gatggttgaa ttagggatcg aaacagatga ggacaatctg
    15001 attgttcgac gggatatgtc cttaactgga aaaaatgttt gtcgagtgaa tggtcatatt
    15061 atcacattag ctaatctgag aagaatcggc agttatttag tagatatcca aggacaaaac
    15121 gaacatcagg aactgctcca gcctgaatca catcttgctt tattggatcg ttttggagat
    15181 gtagctttcc aacagaagaa aaagagttat cagcaagaat atatctctta tcgtgagctg
    15241 gaaaaaaggg tacgaaaagt gcagcaaaat gaaaaatcct atgttcagag aatagatatg
    15301 ctccatttcc agcaagaaga aatagctgct gctgatttgc aagtcggaga agaagagaaa
    15361 ttaaaagaag aaagagaaaa acttagcaat tatcaaaaaa ttgctgatgg attagctgca
    15421 ggttacggag cactgacgaa taatgaacaa aacagtgtag atggtgttgg cttggccgtt
    15481 tcagaactgc aagggatcgc ccatctagat gtggagtatg aagcaattta tgaaaatatc
    15541 caaagtgctt actatttgct gcaagatgca atcggcgata tgagtcgtca gattgattta
    15601 ttggagttag atgagagtcg tttagaagaa gttacgcaac gtttagaatt aatccgacag
    15661 ttgaaacgta aatacggcga atccattgag tcaatattgg cttactatga cgaaatcacc
    15721 gaagagctag cttcttctga tttttcagaa ggtcaattgg acaaaatgaa agaggaacta
    15781 gagcaaaaag agcttctact tcagcaacaa gccgcagatc ttcatgaagc gcgtaaaaaa
    15841 atcgcaaaag aattggaaaa atccattctg catgaattga aaagtctgta tatggaaaac
    15901 actgagtttg aggtacgttt cttaaaagaa gaaaatagac aattgaatca tgatggattt
    15961 gaccaaatcg aattttacat tacgaccaat ccaggagagc cactgaagcc tttggtgaaa
    16021 gtcgcttctg gtggcgaact gtctcggatg ttgcttgctt tgaaaactat tttttcttct
    16081 gagcaaggtg tgacaagtat tatttttgat gaagtggata caggtgtgag cggaagagtt
    16141 gcacaggcca tcgcagataa aatttctcaa atatcgaaat actctcaagt tttgtgcatc
    16201 actcatctgc ctcaagttgc tgcagtcgcc gactatcaat attatattgt gaaagcagtg
    16261 attgatggac gtacgcaaac ttctgtttca gagttgaaaa caaaagaaag agaagaagaa
    16321 attgcacgta tgctggctgg aagtgagatt acaaaactca cactcgaaca tgcaaaagaa
    16381 ctattgaaac tcgcaaaaag gacctgagcc acctcgcagt ggttcaggtc cttttcagca
    16441 gctgatagct atattttat
//
LOCUS       DACEOE010000051        16103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEOE010000051 DACEOE010000000
VERSION     DACEOE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.053"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..712)
                     /locus_tag="IW774_001347"
     CDS             complement(<1..712)
                     /locus_tag="IW774_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1153701.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQ"
     gene            complement(908..3007)
                     /locus_tag="IW774_001348"
     CDS             complement(908..3007)
                     /locus_tag="IW774_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1153702.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3227..4336)
                     /gene="rpoD"
                     /locus_tag="IW774_001349"
     CDS             complement(3227..4336)
                     /gene="rpoD"
                     /locus_tag="IW774_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1153703.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4360..6201)
                     /locus_tag="IW774_001350"
     CDS             complement(4360..6201)
                     /locus_tag="IW774_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1153704.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6383..8776)
                     /locus_tag="IW774_001351"
     CDS             complement(6383..8776)
                     /locus_tag="IW774_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1153705.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(8883..9743)
                     /locus_tag="IW774_001352"
     CDS             complement(8883..9743)
                     /locus_tag="IW774_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1153706.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(9802..10323)
                     /locus_tag="IW774_001353"
     CDS             complement(9802..10323)
                     /locus_tag="IW774_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1153707.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            10643..11362
                     /locus_tag="IW774_001354"
     CDS             10643..11362
                     /locus_tag="IW774_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1153708.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            11388..13979
                     /gene="mprF"
                     /locus_tag="IW774_001355"
     CDS             11388..13979
                     /gene="mprF"
                     /locus_tag="IW774_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1153709.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14167..15354)
                     /gene="tuf"
                     /locus_tag="IW774_001356"
     CDS             complement(14167..15354)
                     /gene="tuf"
                     /locus_tag="IW774_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1153710.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15722..16078
                     /locus_tag="IW774_001357"
     CDS             15722..16078
                     /locus_tag="IW774_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1153711.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4400 a         3345 c         2845 g         5513 t
ORIGIN      
        1 tttgctgatc tgcagaacgt tctaaaatgg ttaaaatcat ttgtgcatca tctgatatag
       61 cttcatatgc tcttggcttc aatgaaagat tcagtacacc gtctggtcgg acaccaatca
      121 ctcgaccttt tatctgctcg cctagacgag gttcattata tcgttcagaa ggatggataa
      181 acccaagata gaaatcttcc gttaaaacgt acgtacctgt taatttcaag cggtatgctg
      241 ttcctatcac atctttgttt ttcatttctt ctgttccata tttgcttaaa gatttgaata
      301 ttttttcatc tgctaaactg ccccaaatac ggtcttttgc atcaactcta agggaaatca
      361 tcaagcgatc ccctttttta ggccatagct ctgtcattgt cggcaattca tcaagagata
      421 cgacaaagtc tttatctgga agaccgatat cgacaaacgc acctaaatct tttcgtgtag
      481 cagtaacgat tccaaaagcg taatgaccga tacggctttt aggaatttgt gtagtgatcc
      541 gattttcatg tttttgattt tggtaaccga atccttcaac tgcttcaccg attttgtgtt
      601 caccttctga tttgcttaag ccaaaggttt gtccgttttt ttggacaaag tagttttttt
      661 cattttcatc aataataata cctgtaaata tagttgctaa taaatcgttc atttttttct
      721 ccctctatag tgaaaggttt tcttaataca tttctttgct atcataacat aatgttatct
      781 acttgaatag ctgagccttg gtttggaaag gtgaaaaaag gagtatgaca acagttgtgt
      841 catactcccc tttttcgaat aaacggtgtt aaacggcagg ctcacagcct ctttcatttg
      901 cctaaaatta attgctccca tttcctccga accatccagt tttacagttg atggttacga
      961 tgtaaaatgg caaagcactt tctccaatgc ctttcatcaa tgcagatttt gttgaagtgg
     1021 catatagatt ataatagcct tccccatttt cgattctggc tttttctaat ctgtaacctc
     1081 cttcaacgat atatccacgt tggatacatg tagcaatcac tttgctttgg acacctggtg
     1141 cccacgtcca tgctggatta gatgcatcgg caatatcact tgctctggca gatttgattg
     1201 gctgattcgc tgagtttgaa gaggaagggg tcgaggatgc cgcttcttgc tgtgctgctt
     1261 gtgcgaccgc agcttgttga gcggcttgag ctgcagctgc tgcttgagcg gcttgttttg
     1321 cttgttcttc agctgctttt ttcgcttctt ctgctttttt cttttcctct tcttcttttt
     1381 tctctttatc agtgagcatc ttatctactt gtttcaattg ttcattcaac tgagcagata
     1441 gatctgtatt tttcactttt tctactgctt cttttgccgt cttgtattgg tcgcgtgttg
     1501 ctgaatccac tacttttccg tctttaaaga ctatagctgt tttttcttta gctgtttgca
     1561 attcagcata ttgattttta gcttctgttt gtgcttgttg aatcaatttt ccaaaatctg
     1621 tgttatccgt ttcgattgct tggatctctt tatctgcttt caacttgatg tcatctgcta
     1681 gatgatcatc gacgatggca ggtgctatga accactcatt cactttttgg atatctcgga
     1741 tcttagattg gatatcttgg taagttgttt ctaactctga atatcctttt tcattcttaa
     1801 gactggataa atcatttttt agttttgata aatcttgatg aatcatatct gcacgaataa
     1861 attgatggtg atcatctgta taaaaggcag caagtttctc atgaagttct tccatttgtt
     1921 gctgtctttt tgcttctgct gctacttcgg cttctacttt ttgatgatgg tcataataag
     1981 cccagcttcc tccaccgagt agaagtactg ctgctaaaac gagataaggt actttttttg
     2041 attttttctt ttgcgcttta tctggatctg ttttttctaa gtttgattct tgatgtgttt
     2101 tttcagtatc tggcacgatt gttattgatt tcgcttctgc ttcaaacagc ttaggctggt
     2161 tctcttcttt ttctattttt gcttcaagat cagtaccctc tttgcttaca gattgatttt
     2221 cagcatcgga ttcactattt atttcatcgg gctctgttga ctttttgtct gtgttttcga
     2281 gtatttcttt tttctctgct tccggcagtt tattcgtttt ttccttttct aattgttttt
     2341 cagtaaatac ttcagttttt tctgtgatat ccgtttttac gtcttttatt tttactgttt
     2401 ctttacctgt ggcgttttct ctatcagagt ctttttcagt ttcttgttcc ttttctggct
     2461 ctactttgtt ttcctgtgca attttttcac tgatcatttt gagcagctct ttcgcgtgct
     2521 cttcttttgt atcttcacga tgtgctttga tataagcgga tagtatcgaa ttttcttcct
     2581 cattatcgac gatttctttc agttcagcta ctcttctttt ttgttttgat tcaagcgttt
     2641 cttcttgatt ctttcctgat tcttccgaag tctcatgaac agttgctttc ttctgttcat
     2701 ttttctctat attagttact tgatcgtgag caggtggttc ttcgtcttct ggttctgctt
     2761 tttcgaataa ttccattaca gattctagag gtaaatcttt caattccgac cattcgattg
     2821 gatcgtttaa ttctgtatca ggaaagactg catcttcagt tgatttatcc tgcatagctg
     2881 catctgtatt tgtttcttca attgattctt ttatttgctg atcttctgca gttatttgat
     2941 gaccgcagtc tggacagata gtattctgct ccattttttt cgatccgcaa tttggacatt
     3001 ttttcatcac ttagcgatcg gctcctttac taggtttacg ctaccattta atctatcata
     3061 ttctatacgt cacgccaata gcttattttt aggatttact taaaaataat gaatgaattt
     3121 ccttcctata gaaaaaaaag cttgagactg cgtagtctca agcttttttt cttttaaaat
     3181 aaaccaaaca gttttactga tttctcttct ggtcaatagt ccacgcttat tctaagaaat
     3241 cttttaattg ttttgaacga gaaggatggc gcaattttct taatgcttta gcctcgatct
     3301 gacgaatacg ttcccgagta acaccaaata ctttccctac ttcttccaat gttctcgtcc
     3361 ggccatcatc taaaccaaaa cgtaagcgaa ggacattctc ttcccgatca gtcaatgtat
     3421 ctagtacatc ttccaattgt tcttttagta actcataagc tgcgtgttct gctggacttg
     3481 ttgcttcctg gtcttctatg aaatcaccta agtgagagtc atcttcttca ccgattggcg
     3541 tttcaaggga aacaggttct tgcgcgattt tcaagatttc tcggactttt tctgttggaa
     3601 gatccatttc cgcaccgatt tcttctggcg taggttctct tccaaggtct tgcagcaatt
     3661 gacgttggat acgtatcaat ttattaattg tttcaaccat atgaactgga atacggatcg
     3721 tacgtgcttg gtcagcgatt gctcgagtga tggcttgacg aatccaccat gtagcatagg
     3781 ttgaaaactt aaatcctttg cggtagtcaa atttttcaac tgccttcatc agtcccatgt
     3841 ttccttcttg gatcaagtca aggaactgca tgcctcgtcc tacataacgt ttagcgatac
     3901 ttactactaa acgaaggttt gcttctgcca gacgctgttt cgcttcttgg tcgccttctt
     3961 cgattttcaa tgctaacgct acttcttctt ccgcagttaa aagggagaca cggccaattt
     4021 ctttcagata catacgaacc gggtcattga tttttacacc ggttggcgca gaaagatctt
     4081 ctgtttttgc ttgttcagct tttttctcag cactcttcaa gctgtgaatt gatggatcac
     4141 cattttcatc caccacactg atacctgcgt cttcaatctt ttggatcaat ttgtccattt
     4201 catctgcatt caaagtgaat ggtgtagcaa gctgattgga taaatcatca tatacaactt
     4261 gtcgtttggg tttgttttct ttgatgaaag ctgctactgc tgcttcatac gtttttttat
     4321 ctgttccatt tgccataaaa gaaggccccc cttgagtagc tatttggctt gttttaactg
     4381 tttcgttaaa ttgatgattt caatcgtcaa ttcaagctca agctgtttgt ttccagttcg
     4441 tctggcttct tgctgcattt gtttttttaa tgttatttca tcagctaagc cagatttgct
     4501 gatcacatct agcaagtctt ggatttctcg tggcgaactt tcttctgcca ccggtaaata
     4561 ttcgatttcc accaccaacc gccttaaatt ttcatcttgc aaaaagtcta aaaactctgc
     4621 aaggttgaat tcataatgtt gtgagacata tgcatcgaat aaaaaataaa cttctgcata
     4681 tgcatcatga ataaactgaa attctcgttc ttttaaaata ttacggacag aaacttcttt
     4741 aaatgcacga tataatagaa tctgctctgc cttttctact tgcgtcaaag gtctcttttt
     4801 ccgtgcagtt tcttgtctcg taacctgagt gtgttgactt tcctgaggtt cttgacgatt
     4861 ttgctgattt gtccttcgaa gatcgcgcag ttgtttttgt atcgtctctc tgccgatctg
     4921 aaactctgtt gacagttgag atagatacat atcttgttcg attggtgaaa caactcggct
     4981 aagttcaact agtaattctt cgaggtattc gatcttgtct ttttcattat ctaaattttt
     5041 cccttgtttt aaataacgtg atttaaaagt aaagactgtt tcgcgcccat gagccactaa
     5101 ttcttggaaa gatgcttctc catacttacg gagatattca tcaggatcaa gcttttctgg
     5161 aacgactacg acagaaagtg ctaatcgact gttttgccga agcaattcaa tccctcgatt
     5221 ggttgcttca aaacctgcgt tgtctccgtc atagcaaaag accaattctt tcgtcagctt
     5281 ttccatttgt tggatctgtt gagctgttag acttgtcccc atagaagcta atccagtttt
     5341 gataccagcc tgccatgcag caatcacatc cataaaccct tcgaacaaca aaatttctcc
     5401 gtttttccgg atatcgcttc ttgctttatc aagattaaat aaaacttgtc ttttattgaa
     5461 cagttctgtt tctggactat tcaagtattt gggctgatct ttttcctgcc ctttttcagg
     5521 tgcaagccag cgtcctgaaa aaccgatcgt ctggccttga gaattacgaa gtggaaacat
     5581 cactctttga taaaaacgat cagacatacg gccatcatca tgcgtgacaa acaatccaga
     5641 gcgttctaac aattcatttg aaattgattc attcttgaat acttgctgaa gaaaagaacg
     5701 ctcattaggt gcaaaaccta tgttgaattc ttcaattaat tctaaagaca acccacgact
     5761 ttgcaaatat tctaaagctg gctgtccagc ttttgtatgc aatagcatgt gatgatagat
     5821 ttccgctgct ttttcatgag cagcaatcag tttccccgta gcagattcag gattttggac
     5881 agctatagta cgctgacgcc attgatcttc taacggtatc tgttcgaagt ctgccacttt
     5941 taccactgct tctggaaaac ttagaccttc tagttcttga agaaaagtaa acacgtttcc
     6001 gcctttacca caaccaaagc aatgaaaaat ctgcttgtct tctgcaacag aaaaagacgg
     6061 tgtacgttct tcatgaaaag gacacagccc caagtaattt ttaccagact ttttcaactg
     6121 gacatattgt ccaatcacct caacgatatt cgtttgactg cgtactgttt cgataacctc
     6181 ttgaggaatc cgttgagcca ttttttcacc ccctgctcta ttgatatctt ttgggtttaa
     6241 cagaagatat ttcaggagaa taaccggtaa aaaatcgcac taactgaaat cagtgcgatt
     6301 ttaaaggaca caattataca tgtttgattg tatcatattt cattgctttt tcaagtataa
     6361 cacatttcta tctcattaag catcaatttg ctactctttt cagtaactga ttttgaatat
     6421 cagccttcca gtacaatact cgaagaacaa tatttacgag catgaataca aagaagtatc
     6481 ctacgctctc tcccaaccct aagtttactt ccaaaattcc atggaacagc attgcagcta
     6541 catatatccc tacgatcgtc gttactgcta ggaagatgcg caatgggttg aagggataac
     6601 aagcttttac aactgccatg caactgatcc caatcagcaa gtaatacatc aaagtagtag
     6661 tttcagcatt tccccatgcg aatgtttttc caagtagata caccgcaatg atattcacta
     6721 ctacaagcaa ggcatttggt aaagcattaa taagtgctgt aggcaaataa cggtaagtga
     6781 tttttctttt atcttgttca aaagataaaa agaaggaagg atacccttca atcgctaaat
     6841 caatcagcgt gatttggata gggataaacg gaaatccaat tgctgtaacg gcacatataa
     6901 tcgataaaat aaatgaataa atcgttttga taaaaaagat gcctgagact ttcgtcacat
     6961 tgttgaccac tcggcgtcct tcaaacagga cttctggcaa tgtagtaaaa tcagagtcca
     7021 acaataccag attcgcaatc tgacgtgttg ctccatcacc ttctgccata gcaatgctgc
     7081 aatctgcttc tcttaacgcc aaaacatcat tcacaccgtc gcctgtcatt gcaaccgttc
     7141 tgccgctttc tttcaattca tttaccagca atttcttttg ctgcggactc actcgtccaa
     7201 aaaccgtata gcgatgagca gcttctcgca cttccatttc ttcagtaatt tgagaaagat
     7261 cgacatacgc atcataattc ggtaatcctg cacgtctagc gatattggag acagtaaaag
     7321 gattgtctcc agaaatcact ttcagatcca caccttcctc atgaagataa gctagtgttt
     7381 cttgagcgtt ttttcggatt ggatcatcga tttcaaacag agcaaccggc tcaacaaatg
     7441 ctggtttttc ttcgttcaat tgctgatttg gagcgatccc caacataagt acgcgatagc
     7501 cattcgcttg ggctgttttt agactgtccg gaagccgatc ttcttccacc aaacgttcag
     7561 gcgctcctac gtacacgact cccagttctg gaaattccat cgcgccccat ttgcgatcag
     7621 aagaaaatgc gactttgttt gtcacatcat accgattaga aagctcaaaa taatctctca
     7681 tagcccgcat agtgatgttg ttatcactgc tttctgccag atacgtcccc ataatttctg
     7741 ggatggcttt ctcgtacagc ggatgtagtg cttctacccg ctggatactc attttccctt
     7801 cagtaatggt ccctgtttta tccaagcata atgtatcgac atgggccagc gtttcaattg
     7861 aatacatgtc ttgaacaagg attcgttttt ttgccagttt ggttacccct gtcgttaatg
     7921 caatacttat tagcaatacc aaaccctttg gaagcatgcc taacaaagct gctgcagatg
     7981 caataacaga aattttgata gaagcatcgc ggatgaacag tgcttccaaa aatagtatga
     8041 tccccaaagg aataatcacg aaactagtga atttggatac tttgcgaatc gaatctacaa
     8101 gttcagaacg aatagctttg tgagtctttg cctcgctagt caagcggaca gcataattat
     8161 ctgcacctac tcggataact tcagccatga cagaaccgct tgtcaaaaag cttccagaaa
     8221 gcaattcagc tccagtttct ttgacaatca aatcagattc gcctgtcaaa agagcttcat
     8281 ttgcttctgc ccgtccagat ataacctgta aatcggctgg tatctgttct cctgctccga
     8341 tttttaccaa atctcctaat accagctctt tagtagaaat cgattgttct ttaccgtccc
     8401 ggatcactag tacttctgat tgggaaacaa tcgataattt ccgtacaaga tttcttgcat
     8461 gtatttcttg ataaattcca ataacgatat tgacgacaat aattgctaaa tacgccatgt
     8521 tggaatacgc acctacaaat aacagacaaa tcccaatacc aaagttcaga aaattgaata
     8581 acgtcaatat attatcgctg aatatttgtt ttgtactttt tgatgcatct tcctggtagt
     8641 cattttgctg gccagaagcc attttctgaa ctacctcttg ctgtgtaagt ccattcattt
     8701 tctttttctc cattttccgc caactttcaa aaataatcac gtgtttaagt ctagcgtgtt
     8761 ccaagattaa aaacaacttg tttttataaa atttatagaa aaacacacga atgattttac
     8821 aaaaaagatg ccaacctttt tgtttgaatc acccgtgtgc tgtttttatt atcaatctat
     8881 ctttacttat attgttcctc caaacggctc atccaccaac ctagtgctgt accggctata
     8941 aaaagaacag gacaagctag taccccagca actcccaatc ccgcgtaatc ggtaggaatg
     9001 atcataatcg ttgaagcaaa aactacacct aaaatgaaat ggaatagctt gctatatgcc
     9061 tttgagaaaa tgtaatccat gatttttgac aatccaagaa cacaaagaat cccaccaatt
     9121 ccgatcggca caatcacttc aagtcgtact tctttaaatc cgtcagccat cgctttatac
     9181 aatcccatat acaccaaaaa attagaaggg ctcaatcctg ggacgatcat acccaaacca
     9241 atcaatccgc cggcaatcag ccatgttcca aaattctgat cgacttgccc gtcaaataat
     9301 tgagcgccgt ataataaaaa caaataagcc agaacaaaag taacaaccat gatgatcaca
     9361 tcagaagtat ctcttccttt tttccctgct tctttccata acgcaggaac agttccagcg
     9421 atacatccga caaaaaacca taatatcgtt gtttcatacg cacctaataa aaaactgact
     9481 aaaaaagaaa gtagaaagat gcctgtcact ccgcctagac caacaggcag aaaaaacaaa
     9541 acattttctt tgaaattttt tgtaaggtgg gctaaaaaag aaatcagccg ctcatacatg
     9601 ccaaagacag ctgctaatgc accaccgctg actcctggca agataaatcc tgaaccgata
     9661 aacattccct tcacaaaccg taacatccag tctttggttg tgtaccccac ttcttgattt
     9721 tccatttttt gccttttttc cactatttgc gcactccttc tttcatttaa ttttattcat
     9781 gattgaacat ttcctgctat tttacttaaa gactttttca taggtctcta ttattttttc
     9841 attcattttg acaatatcag gataaaacat gttacctcca ttatgataga gatagccgcc
     9901 attgtaaagc aaggactgga tccgccaata tcggtattgt ttcccttcct catttcctaa
     9961 aagtgggctc agcacatctt tagaatacgc ttctgctaat tctactgtgt tttctccgcc
    10021 atgatttttt acataggcaa tataatcttt cccaaaatta tatgcttgga cagctgtcca
    10081 taaatcgcat ccttgtttct ttgcttctac tatcatttcc gccaaatatt tgacaccttg
    10141 atcaatactg tcagctggat tatcaatgat ctcagcttct ccatgcagac tttccgagct
    10201 ctgcatgaga tctgtccctt ttccttttga ttcggtcaat attatcgcct tgatcaaatt
    10261 ctcgtagttt tccatatgat aattttttgc agctgttgtt atttctattt gatagctttc
    10321 cactcgtttt accgaacgat aagttcttcc tcataaaaac aacaccgcac caataaccgt
    10381 cagaatgatc atgtaaaaaa gaatacggat aatagataat cgttttcgct tcatttcatc
    10441 acctagttat tttgacatct ttttttatct tactatttct ttatcttctt ggctatttct
    10501 tccttgattt ctgtgtacgc cttaagaaaa aatgaatttt cctgtatttt ttgacatctt
    10561 acgaaaatga tcgttgtttt tcagtaaaaa caattgccca atgactgata taatcgttat
    10621 actttacaca gtaggtgatt atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg
    10681 gagcgaaatt tactatgaaa agagcggtca aggcttccct ctctttttac tgcatggcaa
    10741 tgacggcagc ggccgctttt tttcagaaca ggtccctgta ctcgaacgct actataccgt
    10801 gtatctagta gatagccgag gacatggtcg ttcgacaaac gaagcatcta tgttgaattt
    10861 tcagttaatg gccgaggatc tgaatacgat catgttgctg gaaaaaatcg atcaggcaga
    10921 ttttcttgga tttagtgatg gagcaaatct agcactggta tttgccagca gcttccctaa
    10981 aaaagttcat cgattgattt taaattctgg aaacacgctt gttaaaggcg ttcgtttttc
    11041 tgccagagtc atcagtaata ttcattatgc atgggtatgg ttactctctt tatttcgccc
    11101 aagtcttcgc aaaaatcttt tagttatcaa acttttgctg catgacatcg ggcttacaga
    11161 aaatgattta aaaaaaatca actcccccac tttgatcatc gtgggaaaaa aagatgtaat
    11221 caagctaaaa cattctttgt atatagctaa aactatacca aaagcttcct ttgtgcttgt
    11281 gaaagaacaa ggacatgaat tagctagaaa agatcctgaa cgcttcaatc gagaagttct
    11341 tcaatttctt tctgaaacat aactattagg aatagaaggt gatttatttg ttaaaaaata
    11401 ccatacaatg gctgaaaaac cacctaggac tgttcaaaac aatctttttg atttctgtca
    11461 ttgtcattat tgtcggtgaa ttgatgtcca tcggtaaaac tctatctatc cagcaattag
    11521 gagaaacgtt tgccactata cctgtatgga aaagcggact aatgctattg attggtctta
    11581 tctctgtgtt acctatggtt ggttatgata ttattttgaa tcggatgctt gaacaaaagc
    11641 agaatcctcg ttacctgttt gagacaagct ggctgatcaa tacgatcaac aatattgccg
    11701 gtttcggcgg gtttgttagt gtggggcttc gctctgaact atatggacaa aagaaagaga
    11761 gcagaaacgt gatccaagca ttatcgaaag ttttcttgtt tttgatggct ggactttcta
    11821 tttacagtct gctttctttt ttacttgtag tcttcacgcc agttgctcct tttttgaagc
    11881 aatattggat atggttgatc ggcggcggtc tatattttcc tgtcgtcttt ttatttacta
    11941 ctttcaaaaa gaaaggattc cttggtggta cctcacttaa aactcaagga cagctattgc
    12001 tagtttcatt gttggaatgg accggtgtcc tgtttagttt cctttctgtt ggttatttaa
    12061 tggaaatccg gattgatctg tggcaaacaa ttccattatt cattgctgct tcagtgatag
    12121 gaatcgtgtc tatgattcca ggagaaattg ggagtttcga tgtaatgatg attatcgggt
    12181 tgtccgctat tggtgtgcca agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt
    12241 tctactacat cattccgttt ttgattggaa tcgtcttttt cttcaaaaat atcggttcca
    12301 cttttgatca gcgttattct ggtatcccta aacaattagc aacagaaatt gcgcataaaa
    12361 tcgtggttgt cttattatac ttttctggaa tcatgctagt actttctgcc actatcccgc
    12421 aagcttttac agagtttcgc tggcttcata gtttgaaccc attgaaattt cattttatta
    12481 tccaatttcc tagtatacta ttgggatttc ttttaatcat tatgggcaga gggatcgcag
    12541 cacgtgtgaa acgagcatat ttgcctacaa tcttcttgat tgttctagct ttactttatg
    12601 ttttattaag tgattttagt ttcacaccgg tggttttctt gtctattctg cttctgatca
    12661 ttcttgcttc taaaaacgaa ttatttcggg aacaattgat ctattcatgg gagtggcgta
    12721 cgatcgatgg aatcattatt ggtgcactgt ccttgcttta catcgttatt ggggtataca
    12781 atctgccgga tttccctcat cggcgtcacc attttatttc ctttttctta tttccttctg
    12841 aaaagatttg gttttcggga ttgttggcca tcatcgctgt tagtttcatg atcgttctat
    12901 ttgttcattt ccttcaagga gaaaagaaac agattggcga agcctttaat gaagaaaagg
    12961 ccctgaagat ccttacaact tatggaggaa actccgatag tcaattgatc ttcttaaaag
    13021 ataagcggat gtttgcctat gaaaaagacg gtgaaccaac tgttcttctc caattcgctt
    13081 gcttcaataa taaatgtatt gtaatgggtg atccttctgg taagaaagaa gattttccag
    13141 aagcaatcga agcttttatc gaagaaacag atcgactatg ctatttacca gttttctacg
    13201 aaacaagcga agagattgtt atgattttgc atgaattcgg ctacgatttc attaagatgg
    13261 gggaagaagc ttatgtagac ctgaatagtt ttacaacttc agggaaaaaa atgaagggca
    13321 ctcgtgctgt cttgaatcgg atcgaacgag aaggatttac atttgatgtc ttacaaccac
    13381 cattttctac tgagcaaatg agtactttca aaaatatctc tgataattgg ttgggttcac
    13441 gaaaggaaaa agggttttct ttaggctttt tctctgaaga ttatctacaa agagcgccta
    13501 ttgcagttgt caaaaatacc caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt
    13561 acacgaacaa taaagtagga acgattgatc tgatgcgata tgattctaaa aaagcacctt
    13621 ctggcagtat ggatttcttg ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc
    13681 aatggttcaa tttagggatg gcccctcttt caaatgtcgg aacttccaga aagagttttt
    13741 tacaggaaag gattgcatac ttggtttacg agtttggttc tcatttttat tctttccatg
    13801 gattaagaga atataaaagc aagtacgcta caaactgggt ctcacgttat actctatatt
    13861 ctagagatag ttggattgca tatgttatga ttgctttgct gattattgat aatgcgccag
    13921 ttgagcagac acaaggtaga ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa
    13981 aactgcatac cgatagatga acactctcat gatgaataaa agagactgaa gtaaaacgaa
    14041 agttcatgct tctacaatct cttcttatac aattgaagaa caaagagcgg tccaaacaga
    14101 aatcctgttt ggaccgctct tttattttaa acatttttca ttaccaaaat attcgagtcg
    14161 cgtttcttat gcttcgatct ctgtaacgat acctgaacct actgtccgtc cgccttcacg
    14221 aattgagaat gtcgttccgt tttcaacagc gattggatgg atcaagtcaa catcaatcgt
    14281 cacgttgtca ccaggcatga ccatttctgt atcttctggc aatgtgattg ttcctgtaac
    14341 atcagttgtg cggaagtaga actgtggacg atagttgttg aagaatggag tatgacgtcc
    14401 accttcttct tttgtcaaca cgtacacttc tgctttgaat tttgtatgtg gagtaatgga
    14461 acctggttta gcaagtactt gtccacgttc gatatcgtca cgttggatcc cccgtaacaa
    14521 cacgcctacg ttatccccag cttccccgta atctaacgtt ttacggaaca tttctacacc
    14581 tgttacaact gctttttgtg tttcaggttt gatccctacg atctctacct catcaccgac
    14641 acgaacagca ccacgatcaa tacgacctga ggcaacagtt cctcgacctg taattgagaa
    14701 gacgtcttcc actggcaata gtaatggttt atctgtatca cgttctggtg ttgggatata
    14761 ttcatctact gtatccatca attccataat agcagcttca gcatctggat cgccttgcaa
    14821 tgcttttaat gcggaacctt tgatcacagg agtgtcatcg cctgggaaac catattcgct
    14881 caataactcg cgaacttcca tttctaccaa atcgatcaat tcttcatcat cgactaaatc
    14941 aactttgttc aagaaaacaa tcaggtattt tacaccaact tggcgtgaca ataaaatgtg
    15001 ttcacgtgtt tgaggcatcg gaccgtcagt tgcagaaaca actaagattg caccatccat
    15061 ttgtgctgcc cctgtaatca tgtttttcac atagtccgcg tgtcctggtg catcaatatg
    15121 cgcgtagtgg cgtttttctg tttcatattc tacgtgtgcg gtattgatcg taatcccacg
    15181 ttcacgttct tctggagctg catcaatact agcatagtct tgaggatttg ctaggccttt
    15241 tttgcctaat actgtagtga tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc
    15301 aatcgtacca atgttaacgt gtggtttact tctgtcataa tgttcttttg ccataattta
    15361 caaacctcct aaataatata gatcactgaa aaatttcagc tctcaccttt ttattatagt
    15421 ctttttttga ctaaatcaaa aagaatacgt ccgacttact ttttgtaaga ttcgaactaa
    15481 gcttattata cgtcaatatt ggtcaaagtc aactaagagc actcgtctgt cttatacgcg
    15541 aaaaaagtac cttttataaa taaaaaaatg tgttttttga gaaaaacttc aagatagtcc
    15601 ttaactttct tttgaagcaa taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc
    15661 caacaacttt caatagacga aatcttctat ttcgttaata ataaggaaag aggtgaagta
    15721 catgaaaaaa ttaattggcg tacttgtcgt tttaggcata tttctcggcg gaagttttgc
    15781 agcttatcat tatttttacg gcggcgaagc ttattataca aaaatcacga cgaatggcga
    15841 aatgtcagct acccaagcag ataacggaga gaaattcacg acttatacct acaagcaagc
    15901 ggcttataat aaagatggtg aaaaaaaaca ggtgacgctt cgcgaagaaa gagaacatcc
    15961 attaaagatg aatgcgtatc ttaaactcaa agtcaatcct cgtaaaggtg tattaagctg
    16021 ggaagaaata aaggcttctg aagtgcctaa aaatgcggca gaaaaaatcg ataattagta
    16081 aatggtctta aaattttatt aga
//
LOCUS       DACEOE010000052        16085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEOE010000052 DACEOE010000000
VERSION     DACEOE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.054"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(182..601)
                     /locus_tag="IW774_001358"
     CDS             complement(182..601)
                     /locus_tag="IW774_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1153712.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(603..1187)
                     /locus_tag="IW774_001359"
     CDS             complement(603..1187)
                     /locus_tag="IW774_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1153713.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1234..2709)
                     /locus_tag="IW774_001360"
     CDS             complement(1234..2709)
                     /locus_tag="IW774_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1153714.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            2890..3201
                     /locus_tag="IW774_001361"
     CDS             2890..3201
                     /locus_tag="IW774_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153715.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3316..5652)
                     /locus_tag="IW774_001362"
     CDS             complement(3316..5652)
                     /locus_tag="IW774_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1153716.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            5794..6462
                     /locus_tag="IW774_001363"
     CDS             5794..6462
                     /locus_tag="IW774_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1153717.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6465..7508
                     /locus_tag="IW774_001364"
     CDS             6465..7508
                     /locus_tag="IW774_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1153718.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(7745..8626)
                     /locus_tag="IW774_001365"
     CDS             complement(7745..8626)
                     /locus_tag="IW774_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1153719.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            8785..9513
                     /locus_tag="IW774_001366"
     CDS             8785..9513
                     /locus_tag="IW774_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1153720.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            9669..9986
                     /locus_tag="IW774_001367"
     CDS             9669..9986
                     /locus_tag="IW774_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1153721.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            9979..10587
                     /locus_tag="IW774_001368"
     CDS             9979..10587
                     /locus_tag="IW774_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1153722.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLVWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            10603..11433
                     /locus_tag="IW774_001369"
     CDS             10603..11433
                     /locus_tag="IW774_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1153723.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            11571..12059
                     /locus_tag="IW774_001370"
     CDS             11571..12059
                     /locus_tag="IW774_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1153724.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12110..12982
                     /locus_tag="IW774_001371"
     CDS             12110..12982
                     /locus_tag="IW774_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1153725.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13158..13520
                     /locus_tag="IW774_001372"
     CDS             13158..13520
                     /locus_tag="IW774_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1153726.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13529..14281
                     /locus_tag="IW774_001373"
     CDS             13529..14281
                     /locus_tag="IW774_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153727.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14295..>16085
                     /locus_tag="IW774_001374"
     CDS             14295..>16085
                     /locus_tag="IW774_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1153728.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLK"
BASE COUNT         4996 a         2802 c         3296 g         4991 t
ORIGIN      
        1 ccatgtttac aaaatttttc ttactactcg aaacgaagcg gctttatcac aacctcttga
       61 taaacggcgt tcaatcagct gtccctcggt attagctcaa aaacggcaaa atatgagaag
      121 ctatttttcc atttttttct ctcatactca ggtcataacg ctgatttcac aacctcttct
      181 ttcataatgg tttttcacca tctttataat aaactggttc tttgatatgg aaatctattt
      241 tttccaacca atctaaaatc ttgtaaccta actcttctga catcggaaaa gaggcgttga
      301 tttgtgcatt ggagatatgg tcaacatgga taccggaagt gatcgtacaa cttcccggaa
      361 cggcagtttt gatgatcaca gctatttttt cggctaagac atcgtcttta tggaaccgac
      421 catcgtggct agggaaacgt acagtttgca tctctgtatc acgagaaaat gtagtgactg
      481 ttccgatatg tggtgtatct ccgcctgtta aagtgatcaa taagtcttta ccgattactt
      541 taacagcagc ttggatcgtg aaacctagtt tggttacttc aactatcgtt tctttttcca
      601 tattaatcac cctgccaacg ttttgaatgc tcatttacaa tttcaaattg atccaaaagt
      661 ttcattgtat ggtgattaac aagatcttgg atcgtttgag gctgattata aaaagcagga
      721 attggtggta tgatttgcac acctagtcgt gctaatttag tcaagttttc caagtgaatg
      781 gcatttaatg gcgtttcacg aggaacaatg actaactttc gttgctcctt cattgttaca
      841 tcagcagcac gtgcaatcag gttgtcgcct aaaccaacag agatacctgc gactgttttc
      901 atgcttgccg gtacgatgat cattccatcg gtcaaaaatg atccgctggc gacagcagca
      961 cctaaatctt tgtttgaata atggtaatca aacaggcttt cgaattcagt tagtgataag
     1021 tcggattcca gctttaagtt ctgttttgcc catggactga tgattccgtg tgtttctatc
     1081 atcggatatt cttttagttt cttcactagg ttgatggcat aaatggtgcc tgaagcgcca
     1141 cttacaccaa tcacgatttt tttcttccgt tgtgccaatt gttccatttt ccgcactcct
     1201 ttttatgatg tcaaaacagt tgcagatcat tcattatttc aaatattttt cccaatcttc
     1261 tacttcttgg aattttgcac gtacaaaatg ttctttcata tcgaaaggaa ctgtaccgtc
     1321 aaagatagtt tttgcactta tcccacggaa acgaatagag ccaggattat attctggtaa
     1381 ttctgatggg tccaatggat ggttcctcat tcctgggatc acgatgatat ctttatctcc
     1441 ttggaaacga gtgttgaaag tccaaactac atcgttcatg tcgaaaatat caacatcttc
     1501 atcgactaaa atgactgtct tcaattcttt gaaggctgat aaagcaagaa tagctgcttg
     1561 acgctgaata ccttcatccg cttcgttttc tttacggatt tgcatgatgg tcatcaattt
     1621 accaccacct gcaggaggat tgtagacatt tactactttt cctggaatag ctttatcgac
     1681 caaggcaaga atacttgctt ctgtaggaat accggccata gatacgtgtt cttcactggg
     1741 accaatcgtt gtttgcatga ttggattgtc ttttcggtga gtaactgctt tgactttcac
     1801 tacttgtaca gctggattag catccccagt atagccagga aattctggca ttgcttttcc
     1861 tgagttcgtg ttgatatctt cttgcattgt ttcatttggc atgatttctg cttcaatgat
     1921 gaattcagca cgagcaatac ccacttcatc gactgctata ccatcaacga tttgaatagg
     1981 ttcgttacgc aaagctcctg cacaccatag ttcattgtag ccaagtggag tggtaggtgg
     2041 ttcgaaagtc gtgccaatgg caattgctgg atctaaacca atattgatag tgataggcat
     2101 tggtttattc aaggcttcga attgtttttg gaagtgacca atgtggcgtc cgccaggcat
     2161 gatgtacata ccgatcgtat ctttatcttc tagaaccata cgatggatcg ttacatcgct
     2221 cattgttttt tctggatctg atcctaagac tacacccatt gtgatgtatg gaccagcatc
     2281 ttcaggagta tttgtaggtg caggcaaaat gttgcgaata tcgaagtctg catcggtagc
     2341 tagatgaacg acttcttgtg ctggagcgtc tgctttgtct actttcacag gtttgattgg
     2401 attttctaca gcgtctttca agaaacgacc aagtgttcga taatcatggt gcaaaatttc
     2461 acctacacgt ttacgactag ccattgttcc gatgttcaca cgtgtgccgg ggaaaccttt
     2521 gatattgttg aaagtcatag ttggtccttc ttgagtcgga cgttttactg taccgccagc
     2581 accgatataa cggtaaacac cagataattc tgcttcagga tcgatttcaa tatctgtttc
     2641 gtgatattga cctggaagtg tttttagttc ttcgatgact tttcttaaat catacggttg
     2701 atctgtcatg agagattcct ccttagttgt ctttacctat ctatagtaaa aggtgaaaaa
     2761 gaggaaaaca attcaacttg ttcggtaaag tattctacaa tggaatggat gcgttttttt
     2821 gataatagaa taaactatga tatacttatc actaaaagaa agcgcatact tagtgttgga
     2881 ggaaggaaag tgaaaaaaat ggaggagtca tttcctaaag cagtaaaagt ggaaaatata
     2941 gcgaatattt tgaaagttac atttgaaaac ggagaagtaa aatacgtgaa atctcattgg
     3001 acagaagaga taaccgatgc cttgcaattt ggaaaaaaag gaagaggaaa aagaaaaaat
     3061 cttctcgcac tttctcgtaa tatgtggatt gggactgaag taacgattga agcagatgga
     3121 acagtattca taaatggaaa agacaggtat acgccagaag agttgtggta caaaggaaag
     3181 aaaagtatac cagaattata aaaaataatt aaaaaaaaca gcgcgaaaca aggaacatat
     3241 tacttcctcg tttcttgctg ttttctacta tttcagcgct cttatgactc aaatctaaat
     3301 gtggttgtca caaccttatt ctgcacgcaa agcgatagcg gcgtcttttt tcgctgccat
     3361 tcgtgcagga agatgaccgc caagcatcgt caatatagta gagacgatca ctagaataat
     3421 cgcatggaca ggatttagtt gagcaacatt ttttagatcc gtcatgttgt ataagatgct
     3481 attgataggg aacgtagcta gccatgcaat aaacacacct aatatcccag aagaaatacc
     3541 taaaatacac gtttctgcgt caaatacgcg agtgatgtct tttttacgtg cacctaaagc
     3601 ttttagtaca ccgatttctt ttgtccgttc aatcacagat gtataagtaa tgatactgat
     3661 catgatcata cttgttacta aagagatccc tgcaaaagca ataaggacat aagtgatagc
     3721 atccatcaat ccgccagtca gttcagtcat cgttccagct aaatcagtat aaatgatttt
     3781 atcttcatct gatttgcctt tattgtaatc atctagataa tccaagatct tttctttgtc
     3841 ttcaaaattg ttcggataaa tcatgatact tgaaggcaag ctgtctccac ctaaataact
     3901 aagcaatgtt tgtttggcat tttcgtcaac tttttcagta gttaagacat ttacaccact
     3961 gtctctttga gctttgacga tttctgattc tttgttttcg ttgacgattt gagtcgttaa
     4021 ttgatcgcta taagcgattc ctggagataa gagattcatt gtagaagaag atttcacgcg
     4081 taggatcccc gagatcttca attcatcaga agcattctga tacatcgcat cataatctgt
     4141 attagggata aaattcccag ttggaagttt tgtatagtaa gtattgttgt ttactaattt
     4201 gaatgttgta ccaacgatat catcaaaatc aagtttttcg tcttctttca catcaaatcc
     4261 aagattcttc aatgcgttga tattagtatt attattgccg tctacgatca gcacgacatc
     4321 tgttgctgag gctggataac ttccggcaag taaagaataa ttgtctttta aaaagttttc
     4381 tttgctagtg tcaagctgtg tagggaaact tgacactccc acacctgtca ttgctgacat
     4441 cgtactagat aaagaaagag attccgtatc aggattttga ttcgagaagc tgacaggctg
     4501 tactttgcca tttacatcac gtaaaagatt gattcctgta gtacgggtaa atccgatatt
     4561 attacttaag ttaggatcga tgtctgttac gtaatcgacg tatgtttgat caatcttatt
     4621 cgtgtgctgt gctttatctt cttcgctgac ttttgcagta atcgttttgc tgtctggata
     4681 atccgctttg cttgcaccca atccggcatc gtcacgagtc tggttagtcg ttaccttaga
     4741 aatagtaatc ggaaatttcg ccatcgtttc agcttgcgtg ttatcgattt gtttttggaa
     4801 tccagaagaa agggacagaa cgatcgcgat tccgatgatc ccaatactgg aagcaaagga
     4861 ggtcaagaaa gtacgtcctt tttttgttcg gatattgttg aaggaaagct ttaacgctgt
     4921 ccaaaaactc atctttgttc tgcgtaagtt aaattgatca tcttttggtc gttcgatgtg
     4981 aggatgggaa tctgttaaga tcttaccatc agaaaattca atgattcgat ctgcgtattg
     5041 atgggccagt tcaggattat gtgtgaccat gatcactaat ttttctttcg ataactcttg
     5101 gatcaattcc atgatctgga tacttgtttc tgtatctaac gctccggttg gttcatcgca
     5161 caaaaggata tctgggtcat tggctagtgc tcgagcgatg gccactcgct gcatttgtcc
     5221 accagataac tgatttggct ttttatgcat atgatttgtc aagccgactc ggtgcaatgc
     5281 atcttctgcc cgttttcgtt tttcgtcttt agaaacacca cttaatgtca tcccaagttc
     5341 aacgttttca ataatgccta gatgaccaat caagttgtaa ctttgaaaaa tgaaaccgat
     5401 agagttgttt cggtaggcat cccagtcact gtccttaaag tctttagtag actttccgtt
     5461 gattaccata tcaccggaat cataattatc tagtccacca atcacattca gcatagtcgt
     5521 tttaccagaa ccactaggac ctaaaatcgc tacgaattct ttttgtctga aagcgacaga
     5581 cacaccatcc aatgctttgg tggtggtttc gccgacttta taatattttt ttatatcttt
     5641 taattgaagc ataatcaccc ttctcccgta ttgaaatatt cggtatacta ataagtatat
     5701 cagaacaatg atcgtagtat acgaaataaa tgttaactga agatgaacta caagaaaaaa
     5761 tctagaaatt tttcaaaaaa cagagggaga tcaatgaatc gtattttagt agtagaagat
     5821 gatccgcaat tacagttgct ttaccgttct gtgttggaac gagcgggctt ttcagtcatc
     5881 accgcattga atggagagga tgcgctgaaa aaactagaag cgacacgtat agaattaatc
     5941 attacagata tcatgatgcc gaaaatggat ggctatgact tgctagactc attacgtagc
     6001 accagatttg acacacctgt tctaatgatt acggcaaagg ccgattttga ggataagaaa
     6061 cgcgggttca aattaggagc agatgattat atgacaaaac ccatcgatgt caatgagatg
     6121 atcctacgtg tggaagcatt gcttcgacgt tcaaagatca attacagcag tagcttgata
     6181 atcggacaaa caaaattaga gctagatgct tatacagtgt tacaagatca gcattcgttg
     6241 attctgcctc aaaaagaatt tctgcttctg tataaattac tgtcttatcc caacaagatc
     6301 ttcactcgcc aacagttgat ggatgatatt tggggattag atacgaatac agaagaacga
     6361 acgatcgatg tacatatcaa acgtttaagg acccgttttg aaaagacgga agattttcaa
     6421 atcatcacag tacggggact aggctataag gcggttattt aaagatgaaa aaaaagctat
     6481 tttcacaatt atggatctat tttgctagta ttgtcttcat ttcgatctta gtgacactgc
     6541 tgtgttttct cggttttatt ttcttattgt ctcgacattc tatctcgcct gctgaatcgg
     6601 ctggaaaacc aacgttatac ccgttattcg tctttgcggg gttcagtatg gtcgtgggta
     6661 caggaatatc gatttttgtt gggcggcgta tcctgcatcc aatcagtgcg ctagggacaa
     6721 atatgagtct ggtagcaacg ggagattttt cgattcgaat ggatgaacag cagaaagtag
     6781 cagaagtaca gcaactgtat aaagatttca acgttatggt ccaagaactc aatagtatcg
     6841 agacactgag gaatgacttt gtgtccagtg tatcccatga attcaaaaca ccactagcaa
     6901 caatccaagg atatgttcaa ttgttacaag ccccaaatct ctctgacgaa gaacgccaaa
     6961 tatttttata tcggattatc gaaagtatca cgcagctttc acaattaaca gagaacatct
     7021 taaaattgaa caaactggaa aaccagcgta tccaactgga aaagaaagag tatcgattag
     7081 atgaacaaat cagagaagta atcgtttttt ttcagcctaa atgggagaaa gaacagctag
     7141 aattggatat cgaacttgct gcagtaaatt acacaggcaa tgaagagttt ttgtaccaag
     7201 tgtggctgaa tatcatggat aacgccataa agtacaatca aatcaatgga cagattcata
     7261 tcaaactatt tgagacagcc acagagattg tactggaagt gaccgattca ggtgtaggaa
     7321 tgaatgaaga gaccagagat cgaatgtttg aaaaatttta tcaaggagat acaagtcgtc
     7381 aaatttctgg gaatgggcta ggactttcac tggttaaaaa aattctagaa ctgcatgatg
     7441 gacgtattga ttatagcagt atcgaaggag taggaacgac agcaatgata cggttaagta
     7501 aacaatagga aaaagaataa aacccctaaa aattctattg aaggttttta ggggttttta
     7561 gtcgtttttt tgtgattagg gaaatgttct tacggttctt ttggcataaa aatcgctgct
     7621 ttcttattga actacgagaa atgttagagg tgtcattgaa acaaggacat gatgcaagaa
     7681 agagagcgaa agtcatgcgt cgctctcttt ttttgggtgg atagatggac agaaaatgta
     7741 gggcttagtt ttcttcttga tttaaatact cccaaaatgt atctgaagcc aaagaatgct
     7801 caccgacttt acgagtaaat gcaagataac tctctgttgt tggagaaata gggagaatca
     7861 cagtattttc ttttaataga ttcttgactg ttttttccat aacaatagca atccccatgt
     7921 catttgcaat catattgatg atcaagtcga ttcttgctcc tttataagag atttttggtt
     7981 caaatccagc ttcgcgacac agatctagta caggttgata caagttcgta gtggggccaa
     8041 ggagcaaaaa gttctccttt ttcagttcgc ttagatccaa tttcttttta ctagctaaag
     8101 gatgattttt aggtagaact gctacaaaat cgtctgtttc agtaggcaac cactcaatcg
     8161 aacttggtgg ggcctggaaa cttctagcaa agtggatcgt gtcactatgt tcattggcgt
     8221 cattgtaagt taattcgaca ctttccactt ctttcaactg gactgtaaat tcaggatggc
     8281 gtttcaaaaa ctcggtgatt ttggaaaaac ctttataatt gatcattgtc ggtatcgtta
     8341 agatatttaa tgaaagattg ttgtctttcg tataggtttc aagcacttct tgcatagcat
     8401 ggtattgttc tacgacattt tttacatatg ggagcaataa gcttccagct tcggtcaatt
     8461 cgatttttct atgggcgcgt tcgaataatt tgacgtctaa ttctgtttct aacgaaagaa
     8521 tctgtttaga aatatttcct tgggtggtga attgtcgatc agcagttttg gtgaaattca
     8581 ttgtttcggc taaatcaaga aaaacttcga atttatggat atccatcata ttcctcctac
     8641 ttattccaat aaagaatact ttaacacaaa aaatgaattg gtgtaatctt ctctgtctgt
     8701 tacaatcttt cttgcagcga aagcaataat tacagaaatt tttctgattt ttttcgcttc
     8761 gtagaaaaag agaggagttt tttgatgcca actggagttt tgatcaatgt aggttccgtt
     8821 ttgtttggcg gactaattgg cggattagta ggaaataaat taagtgaaca ttttaaagca
     8881 cagctgacga tggtttttgg tgtgtgttcg atggggatgg gaatctattc cattgcgccg
     8941 atgaaaaata tgccggctgt tatttttgct ttagtgatag gaacagcgat tggtttgatt
     9001 gttcatttag gtaatgggat aaataagggg gctgcgctga tgcaagtacc tatttctaaa
     9061 atcttcccta gtgaaaagct tggtatgacc cacgatgaat ttatcagcac attagtgacg
     9121 gtcattgttc tattttgtgc aagtggtacg gggatctatg gaagtttaga ttctgggatg
     9181 acaggagaca gtacgatttt gatttctaaa tctgtccttg attttttcac agcagctatt
     9241 tttgcgtgta atttagggta tgtcgtttct gtgatagcaa ttccccaatt cattattttt
     9301 tatattttgt tcttattggc aaaatttatt tatccattga ctacaccaga catgatctta
     9361 gattttaaag cttgcggcgg attcttgatg gtcgctaccg gttttagaat gatcaacgtg
     9421 aaaatgttcc cagtagctga catgatacct gcaatgatcg ttatcatgcc actgagctgg
     9481 atgtggacaa attggattat gccactgtta taataaaaat caaagagaag ccattttttg
     9541 ctgactaaaa aggatcaaat cttttagtca gtttttttgt atttgacaaa ctgattttga
     9601 aactatatta tatgttgtat aatatagatt gaaataaaat attacacttg agaatagaag
     9661 gtgacgtaat gcacatccag attccaactg ttcttcttga tggtactgtg ctagcagcat
     9721 taagcaagga agatatgtat gggtatgcct taacaaaaat cgtacaagaa agtctctctg
     9781 tcagtgaatc tacactatat ccggttttga gaagattgaa aaaagaagga tatcttgaga
     9841 cctatgatga accttttgaa ggcagaaacc gtcgatatta ctcgttgaca ccaatgggaa
     9901 gtcgtcattt agaagaaatt attgatgaat ggattgtttt tcgagattct ttagaccggt
     9961 tattggagga gagcacaaat gaatgaatat ttgaatgaag ttgtggatca tcttcaagaa
    10021 attcctgaag acgatcgttt tgatttgatc cagtattatg aggaatattt tttagattct
    10081 ggaaaaacat tagatcagat cattgatgaa tatggtacac caaagcagtt tgccttaaaa
    10141 ttaaaaatca gttacttttc agaagaagaa caagcagaaa acgagttagt gaaaaatcca
    10201 aaaagacaaa ttcgcctggt ttggttaatc attatcggtt tatttgcttc tcctgtcctc
    10261 attccactag cactggggtt tatattggtt attgggggac tattggttgc tttactagcg
    10321 ctcattttta gtatctatgt tttatgtatc agtatcttag gtgttggcct attcatgatc
    10381 atctcaggaa tcagtgtgtt tggacaatcg attctgagtg gattattctt tatcggtgtc
    10441 ggtctgttcg ccacaggaag cgcagtattt atttcgccac tgttgctgaa aggaacaaaa
    10501 gggctttttt atgtcatgat gaactttgtt aaatggatgg gacgacgttt tattacaaaa
    10561 agagatctgc attcagcggg cgtttgaagg aggacacata aattgagatt gaaacatttt
    10621 ttgattaccg gtattggtct gatgcttact ggtggcctcc tagcaggtgc aacttataca
    10681 ttaggcgctc aaaagagtct gacttgggat aatggtcctc ggttgatcga tatggttact
    10741 gagacaaaaa aaatcaagga tgcaaacaaa atcgtcatcc atgcagaaaa tcaaacagtt
    10801 tcggtcatac gcggattgga ttttgagata aaaacgactt atgataagac agaaaagcca
    10861 gaaataaagg agaaaaatgg cgtgttgact gtgaatgcag tcaaaaaatc aacaggtgca
    10921 atcattagtt tagaagacac aagtgcacaa atcactattg ttgtccctgc agatcatgaa
    10981 ttatctgaat tgacaatcga agggaataat agtgcaattg atatcaacga agtatcaatc
    11041 aaaaaagtga atactaagac acatgatggc tgggtatcgt tcaatgaatt tacaggagga
    11101 acaataactg cgaaagatga attaggctca cttcgattag atgaggccaa tgtggataac
    11161 ttgatcgtca caggtgaaaa cagcaacttc catctagaag acagtttgat cagcaaagaa
    11221 ggaaaagtca tcctaaacgg tggcaattta tctttgcatg agatggaaaa cagtggctat
    11281 actatccaaa cgtctggtga atcttatatt gaaaaagact accagccaat aacaaatact
    11341 ttgttagaac aaggacagaa aaagctaaag atcactgcta aagacacatc gatcaatatt
    11401 acgacggatc aagaggacaa tgaaacagat tgatcctgta ctcaactgga tattttaaga
    11461 aaaaacagca aagatccttc caatctttgc tgtttttcat cgcttaacgc acataactat
    11521 agtaaaataa gaaagaaaat atctaaaaag aaaacagggt gaaagagtat atgaaggtag
    11581 aagaaggtca aagagaaaag ttaaaaacag aaatggaaag attacatact tatatcactc
    11641 aactaagcca aaccttctat gatccagaca aagaaaaagt gatggttaat tacccgaaca
    11701 attcagaagg tcgccaatta gagcaagtgt accatgaagt tttcaaacat ttattgacgg
    11761 tgaagaaaga gctggattat tattctttac cgattatcga tacaggaata ttgaagtatg
    11821 atgggaagaa ggaacgattt atctttaaat ctgttcggga agatttgcct ctttcagcag
    11881 ggatggatct agaggtcttg gtagaagact attttacaga agaaaagcac tgggttcgaa
    11941 caaaattgga ttatttaccg caggcagccg gagggacaca ggcaagcggt tggtacatca
    12001 ctgaagacaa agagttagaa ctagaaggtg taatggcgcg tattcgaaaa aaaaattaaa
    12061 aaagtaacat tagatattta ttgaaggata aaggagcgca ggaagtagca tgagagaaaa
    12121 aagagcattt tatatcaatg gttatgcagg gattgtcgga ttggtcgtat tagctttagt
    12181 tggattgttt ttcttctatt taggtatgtg gcaagcgaaa gtttgggcac tttttttaag
    12241 tatttttctt tggttgatca cgttattgtt gttaagttcg gcaactgtag tcagtcctaa
    12301 tcaagcaaaa gtaattttgt tttttggaca atatttaggt acgattcgag aaaatggctt
    12361 tttccttacg atacctttgg cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa
    12421 tagttctgtt ttaaaagtca atgatttgga tggaaacccc atcgaaatct cagcagtagt
    12481 agtctttaaa gtcgttgata cagcaaaggc attattcgat gtagcgtatt atcaagattt
    12541 tgtagaaatc caaagtgaga cagccattcg gcatatcgct agccaatacc cgtatgatac
    12601 attcaatgag aatgatttga cgcttcgagg aaacacgacg gcagtgtctg acgagttgca
    12661 aaaagagctg caagagcgtt tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa
    12721 ccatttagcc tatgcaacag aaatcgctag tgccatgctc caacgtcaac aagcaaaagc
    12781 cattttgtct gctcgacaaa ccattgttga aggagctgtc acaatcactc aaatggcctt
    12841 agagcaaatc gaagatggac aagaaatcaa tttcaccgat gatcggaaag tccaattgat
    12901 caataattta cttgtctcaa tcattacaga caaaggaacg caacctgtta tcaatacagg
    12961 cgacatgaaa gaccgtaagt gacatacttt ttgtaagata ggaagaagga gtttaggacg
    13021 gattattgtc ttgaactctt tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag
    13081 aatgatagtt gattcgaagg cagaattgtt cgcttttttc tataatcagt atataaagta
    13141 agagagaagg ggaaaaagtg aaaaaacttc tgatcggtct acttgttttc atcgttgtgg
    13201 tacttgcagg ggtgaaaatc gcagattacg tagttatggg tggagaagaa tactatgtac
    13261 aaattacgac agatggtaca aaaacagttt caaaatcaga ctctggacaa gaaacagtca
    13321 actatagata tatcctgcct ggctatacga aagagggtaa ggaaaaagaa ctggacttca
    13381 agggacaatt gactcgaccg cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa
    13441 ataaaggtgt cacttcgtat gaagaagtcg aaaaaaaaga cgtgccgaaa acagcactgg
    13501 aaaaactaat taaagggtag ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac
    13561 aaaagtatac ggaaaaaaca atgagaaaaa gacgcaagca ttaagcggca tcagcttcga
    13621 tgtagaaaaa ggcgaattta ttgggatcat gggcgcttct ggttctggta agtcgacact
    13681 tttgaatatt ttatccacat tagataaacc aacagatggg catatccgaa tcaatcaaaa
    13741 agatgtgacc acattaaaag gaaatcaact agcagatttt cgtgcgaatg agattggctt
    13801 tattttccaa gatttcaatt tattagaaag cttgacagca caagaaaata tcgctgttcc
    13861 tctttcttta caaggcgtca gaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga
    13921 acgtttatcc atctctcata ttttggaaag ctatccttct gagatttccg gtggacaaaa
    13981 acaacgggtc gccgccgcac gtgcattgat cacacaacca acgattctat taggggatga
    14041 gcctacaggt gcgctggatt ccaaaagtgc ccgggatctt ttagatacga tggacgaatt
    14101 gaatacaaaa gatcatgtat cgattcttct tgtgacgcat gatccgtttt ctgcaagtta
    14161 ttgccaacgt attttattca tcaaggatgg cggtatccat caagaagtga aacgtggaga
    14221 tcaaagccgt gattccttct atcgagaaat actatctatt cttggaaact tggaacagta
    14281 gggggagatt gactatgctt tggaaattat cacttactgg gatcaagagc cgtttaagag
    14341 actacttggt gttattctca ggattagtta tggcatctgc tattttttac atgtttgaat
    14401 caatggcaag caacgaagct tttttgaaaa gtaatacgat cgttgcggct acagttatca
    14461 ttttccgatt cggttctgtt ttactaggga tcatcacatt cgtttatatt ttatatgcaa
    14521 attccttttt gatgacgatg cggcaaaaag actatgcaat gtttatgatg ctgggagcaa
    14581 aaggcagaaa aatcgcacag atgatttttt ctgaaacatt cttagtaggg atcgcagcta
    14641 ctttagtggg ttcagctgtc ggtgtaggat tgacaagtat cgtccaccga ttattagtgg
    14701 atcagttaga tctcaatatt tcgcatttta caccttttag tattcacggg ttgttgatca
    14761 ctgttttgtt ttttgctgtt ttgtttttat tagctgctgt tgtcaatgct ttttcgattg
    14821 tcaaaaaacc gattttagca ttgattcgtg cggatcagac accaacaaga atcaagcagc
    14881 ataaattctt gtttttcctt gaagtagtac tgggaattgc ttttctaggc tgcggttact
    14941 atatgatgaa acacgtgggg acctatcaag tctttggtat aggggtagcc ttagttacga
    15001 tcgtattagg aacttatttc attttccatt ctgtgatcat tttcttttta tcattactaa
    15061 agaaatcaga acagatttca ttgaaaaaat tgaacaattt cacattgtca cagttaaact
    15121 ttcgtattcg tgaatataca caaatgctct caatggtcgc tatgctgttt gccttggcac
    15181 tgggcgcttt gacggtagga ttaggtttta aaaatgaaat tgcagacttg acagaaaaca
    15241 attctggata tgatctagtg atgaataatc ctcgggcaga agatcagcaa aaaatcaaag
    15301 aactttcacc aacactttct gcttcttata ctcaaaaaga agatgcggga acgatttact
    15361 ataatgcaag tgaatttgcg gctcaaccat tgatcatgat aaaacatgaa gatggaacac
    15421 cgccgaagat cacgaaagaa aaagttcctg ctaaaaaaat gaatgaactg actgtacaag
    15481 aggaacttcg aagctatgaa ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc
    15541 aatcagaatt tgatcaactg aacttgccac aatccacttt acagctagtt caggtaaaag
    15601 acttccaagc aaacctgaaa ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc
    15661 catctttgca aaaagtagag tacagcaacc agaaatatga aatgtatgaa gtgtataatg
    15721 gtctattctc tggttttgaa tttatgggat tcttcctagg tttagctttc ttgaccatgc
    15781 ttgctagctg tttgatgttc aagatcttgt caggaagcaa gagtgacatt gctcgttatg
    15841 aaatgcttga aaagatagga acacgacgcg ggttgcttaa acaatcgatc cgtagagaag
    15901 taggcgtgtt attcttagca ccaggaatct taggtgtagt ccatgtctta ttcggattac
    15961 aaatgtttgg tctattcatg caaaatccat acaaagatat ttgggttccg tttacgatat
    16021 tcttcgtatt gtatttcatc tactatgtac tgacgacatg gttatatact ggaatcgtat
    16081 tgaaa
//
LOCUS       DACEOE010000053        16060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEOE010000053 DACEOE010000000
VERSION     DACEOE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.055"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(131..1210)
                     /locus_tag="IW774_001375"
     CDS             complement(131..1210)
                     /locus_tag="IW774_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1153729.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(1296..2255)
                     /locus_tag="IW774_001376"
     CDS             complement(1296..2255)
                     /locus_tag="IW774_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1153730.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(2267..3790)
                     /locus_tag="IW774_001377"
     CDS             complement(2267..3790)
                     /locus_tag="IW774_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1153731.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(3800..4363)
                     /locus_tag="IW774_001378"
     CDS             complement(3800..4363)
                     /locus_tag="IW774_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1153732.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(4369..5232)
                     /locus_tag="IW774_001379"
     CDS             complement(4369..5232)
                     /locus_tag="IW774_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1153733.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(5246..6142)
                     /locus_tag="IW774_001380"
     CDS             complement(5246..6142)
                     /locus_tag="IW774_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1153734.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(6317..7588)
                     /locus_tag="IW774_001381"
     CDS             complement(6317..7588)
                     /locus_tag="IW774_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1153735.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(7578..9095)
                     /locus_tag="IW774_001382"
     CDS             complement(7578..9095)
                     /locus_tag="IW774_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1153736.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            9293..10183
                     /locus_tag="IW774_001383"
     CDS             9293..10183
                     /locus_tag="IW774_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1153737.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(10256..11080)
                     /gene="nadE"
                     /locus_tag="IW774_001384"
     CDS             complement(10256..11080)
                     /gene="nadE"
                     /locus_tag="IW774_001384"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1153738.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(11092..12564)
                     /locus_tag="IW774_001385"
     CDS             complement(11092..12564)
                     /locus_tag="IW774_001385"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1153739.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(13201..13563)
                     /locus_tag="IW774_001386"
     CDS             complement(13201..13563)
                     /locus_tag="IW774_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1153740.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            13786..14538
                     /locus_tag="IW774_001387"
     CDS             13786..14538
                     /locus_tag="IW774_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1153741.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(14678..15601)
                     /locus_tag="IW774_001388"
     CDS             complement(14678..15601)
                     /locus_tag="IW774_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1153742.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(15616..15888)
                     /locus_tag="IW774_001389"
     CDS             complement(15616..15888)
                     /locus_tag="IW774_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153743.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         4917 a         3295 c         2612 g         5236 t
ORIGIN      
        1 gttggtagga gaacaccgtt tagtggagag gtggtgaaag aaacgttcag ccttgagcga
       61 tgacaaaagc tctcacaaac attgcgacga gtaaccgtag gagcaataag aggcggtaaa
      121 aacccgtctc ttatttttcg ttttctttca ctgtttcccg ttcaactagt acaggttcat
      181 agagaatatt tggctgtgcc tgtccttttt caatcgttgt gacgatccac cgtgcggcat
      241 cttgtcccaa cttttctttt ggatgcgtca atgttgtcaa ttttttattc cctaaatggc
      301 ttaaagaaga atcgtctgtt cctacgatcg ataggtcatc tggaatatga tagccatttt
      361 ctgtcaactt atctagcatt aaactagcta tttgatcgtt atagcaaatg atgccatcta
      421 aagcttgttc ttctttcaac cgttgtactg ttttttcagc gattgtttct cgtgtttccg
      481 tggtgaaagt aatgatatct tccgtcaaaa aacgcacatt atactgttca cacgcgctga
      541 taaaaccttt catcctgtat tttccttgaa gatcgtcgat ttttgtgatg aacaacagac
      601 gctgatgtcc tttttttatc aaatattctg ctgccataaa accgactttc acatcatcta
      661 aacaaatata agaggcattt aactcttcat aagcagcatt gatcatcacc atcggaatgc
      721 cttgttctct caaagcaaca taaagtgcta agtttgggtt atactgatta cttttcgtag
      781 gctcaacgat caaaccatct accccataag caatcatttt cttcaaacac tctttctcca
      841 attcgtgatc gttattcgta ctggctaaaa gcaatgaata cccagattct cgtaattcat
      901 tttcgattcc tcggataatc gacggaaaaa tataatcaga gagatatgtg gtgatgacac
      961 cgatcgtttt tgttctgttc aatggtgttt tttctttctt atagctgtca tctacatatg
     1021 tgcccgaccc tttttcttta cgcagatagc cctcatttac caacacagcg attgcttgcc
     1081 gaatcgtatg tctgctgacg tcatatattt cttgcagctt gaattctgag ggaatggttg
     1141 tatttacttc gtattcctca cgaaggatct tttgttttat ctcttctgca atatcttgat
     1201 attttgccat ttgccttgcc cctttttatt tgtacgtaca tatatcataa cgagtctgca
     1261 aggtttattc aagcaaaaat caaggtttct tgtgctcata aggcatacgg aacaggttca
     1321 ccaaattctg gaaatccttg ctcattccac gtaaagcatt gcgcgttcgc gtgccgatta
     1381 ggattgtcta acggatcccc tttttggttc ttctctggtc gagcatgata gatcaaaata
     1441 tcaaagcacc cgtcttctga aaccgtaaac gagttgtgac caggaccaaa ttgcttattt
     1501 ttttcagaag aaacgaatac tggctccgat aatttatgcc atgattcttt taataaaaga
     1561 ttcttgtgct catcagccca cagcaggccc atggcatagt tttcatctgt tgcactacca
     1621 gaatacgtaa tgaatatttt cccattgcga gtaaggactg ctggaccttc atttactgaa
     1681 aaaccccgtt tttcccattc atactcaggt atagaaagga ggatctgctt cccctttaac
     1741 gtccaaggat tatccatttc ggatagataa agattagaat tcccagtaat ttcaggatct
     1801 tgttgggccc acacataata tagctttcct tgagaagaga aagttgttcc gtctaaactg
     1861 aaagtttccc ttttcgtttt tatttgtccc ttttctatcc aatctgtatt cattggatct
     1921 atatgctgat tttcaagtac gaacatgcgg tgatgatgag attcatcacg tatagtttta
     1981 tgattgcttg cagcaaagta aatataccat tttccattga tataatggac ttcaggtgcc
     2041 cagatcaatt cactcatcgg tccttcttca tgagcctgcc atacaatcag gcttgcagca
     2101 tgttctaatt cattcagtga ctttgccctt cttaattcaa tcacttggta gcctggaacc
     2161 gatcctgtaa aataataata accatctgta tgtttatata cccaaggatc tgcccgttgc
     2221 cgaacgatag gattattata aagtgtcttc atcataacaa atttcctcac attttcttta
     2281 ttctaattac attccaagag tatttttcta tccctacatt taattcgtca tgtttttcta
     2341 ttgaaaaata gtttatttca ggttttattt tttctggttc ttcacgtgta tttttgtcca
     2401 acagatcgct actggttaat gtgtagtgct ctccttgatc tagcaaattg aaatcatgtt
     2461 caatcgttat cttttgttgt tcatttgtgc gattgatcaa gaaaatagtc agctgattgc
     2521 cgttatctac gatcacactg tccacataag gaacattttt tatcttttca caatcataaa
     2581 cagggcaaat gatttgttgt ttcaatactt gacctttagc atatttcgat aagtgttgca
     2641 tcacataata agttggattc ctccatgctt gtcctttagc atctgtcagt attaaaggta
     2701 ttgtgtttac taacaatgat tgacaactaa tctttacgcg atctgcgtgt cgtaaaatag
     2761 ccagccctaa tgaaccaaat aataatgtat cttccatagt aaagtaacac caatcgatgg
     2821 gactaccgac ttcaaaatcc ctatgtcttt tttccggttg ggcatgtaca ttccattcat
     2881 caaacgctag atacattgtt ttctcacttc tcaaatatcc ttttacataa tcacaagtag
     2941 caacgatttc ttcaatttga cgatcgaatc gataagcttt tgcaaggtat gtcgctgtat
     3001 catcacgctc atttttagga ggtttagtca agtcttcttc atcatactta tcaatatagt
     3061 tatgcaaagc taaataatct acattctcat aagcttcttc taatgcgata cgatcccatt
     3121 cagggtacga tggtaaacgt ggcgtagagc ttccaactaa gattgtttca attgaatcat
     3181 ccactaactt catgactttt gaggcttcat ttgctaacct accatattca taagcactct
     3241 tagcccccac ttgccatgga ccgtctagct cattccctaa acaccataat ttaacgccaa
     3301 aaggcttttt ttgcccattt tttattctta aatcactcca gaaggttcct tttggaaaat
     3361 tacaatattc tactaaattt tttgcttcat caattccttt tgttcctaaa ttcaccgtga
     3421 atatcggttt cgcttgtaca tgactgatcc atttaaaaaa ttcgtggagt ccaaaagaat
     3481 ttggttctat acctcgccat gctaaatcaa ttttttttgg tcgttgttct ttcctaccta
     3541 cagtatccac ccaatcataa ccagatgtaa agtttcctcc aggatagcga atgacaccta
     3601 attctaattc ctttacataa gagaggacat cttgtctaaa cccgtcacca tcagctgttt
     3661 catggcccgg ttcataaatc cctgtataga cgacacttcc catatgctca acaaatgagc
     3721 caaacagccg agaactgatc tctcctaccg taaaatcctc tctgattttt actttcactt
     3781 cttcgtccaa cagctttccc tacctttcat ttcctatagt caagataata gatacaagtt
     3841 tccatacgcc accagcaaag ataaaaataa aaggagggaa aagaaatatg agcagtacaa
     3901 atcctaataa caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat actttccatg
     3961 cactttgttc aaaaatttgt aaaaatgtga tatctttgtt ttcagaaatc acatatatcc
     4021 aggataaaag aaacatggaa taaataagta aaactacata tccactaatt gaaagtacga
     4081 ttgtctgttc ttctaataat tgattaacaa gaaaataaat aatgatcgtt aacagactaa
     4141 ggaacaagtt cttaccaaaa tgttgcttta ttccttgaaa gaaaaaataa atgatatttc
     4201 ctgatccctc acctttccat tgcgccatta cataaaaaac agcacatgta gcaggcacga
     4261 tagtaataac aggcaaacac acgattaccc agagaatacc taataagatg taatcagaaa
     4321 ccacaatcaa ctgtccataa agtttagtct caactatttt catcttcctc actctttcat
     4381 tccagacata gctgttcctt gaacaaaata tttctgtgca aaaatataaa ccactagtaa
     4441 tggcagcatt gaaataaccg ttccagccat tacaggtcca taaaaagaaa tgttctgccc
     4501 tgtaaataaa gctaaccctg ctggtaaagt tctcattttt gaactacttg tcaatatcaa
     4561 tggataaagc aagtcattcc aactattcat cagcgtgaaa atccctaatg ataaaagagt
     4621 aggtttgctc aatggcaaca tgattttcaa aaaaatatta aactcactta atccatcaat
     4681 gcgtgctgcc tcttccaaag aatctggtaa agtaataaaa aaggaacgca tcatgaagat
     4741 accaaatgca gttgtcatac gaggaatgat caaacctgca taagtatcaa gcatgccaag
     4801 caaattgact tcaataaata gaggaatcat gaagacttgg aatgggatca tcattgtcag
     4861 caaaacgaaa tagaacaaaa ctgattttcc tttgaaacgc atacgagcaa atgcataccc
     4921 cgcaagagaa tcaaagaata cagaagtgat cactgtccct ccagcaaaaa tcactgtatt
     4981 tttgatgtaa tctaataaag ggatcgttgt ccaaacctta atatagttat ctagggtata
     5041 gctatctgca aatattctag gcggaaatga gataatgtct ttttcgtgtt taaaagaaga
     5101 aataatcagc cacaaaaaag gaaacgcaat aaccaacaat ataactagca tactaatcgt
     5161 tcgaatgatt tttttaccac taactgcttt tcgactattc acagtaactg taatcgagct
     5221 ttctggtgcc atacaggttt ccctcctatt tcatttgttt ttctttccgt gacatgtaca
     5281 cattagtgga gatagctata gttgcaacaa tgataaacaa aaccgaagac agtgctgatg
     5341 catagcccaa ttcataaggt gctgtaaatc ctcgactgta aatatattga acagctgttt
     5401 ctgtcttaaa ttgtgggcca ccagcggttg caacatatac ttggtcaaac acttgcatcg
     5461 aaccaatcag attcgttaaa atacaaaaac ctaaggaggg aacgatctga ggtagcgtaa
     5521 tgttaaaaaa ctgctgtcga tttgttgcac catccatttg agcagattca tacaaagagg
     5581 gagagattcc ctgaatagca gtcaagagaa tcgtcatagt aacaccaaaa ttcttccaaa
     5641 cagtcatgat ggctaccgtc ggcatagcaa gggttggatc gcgaaaaaat tgagggatcg
     5701 gcaagccgac ttgtgaaaac aagtaaggaa tcgcccctac attcggatca agcaacatag
     5761 aaaaaataat cgcaattgac gtcaatgaac aaataactgg aatgtaaaat gtcgaacgac
     5821 aaaaacgatt gaatctggta tttttagata atgctactgc tgctaataaa ccaacgataa
     5881 tttgtgttgg cgtttcgagt acggtgaaat ataaagaatg aatcaacgaa ttaactgctc
     5941 gttcatcact aaatatacgt gtgaaatttt caaaacttac aaaatttctt gatgtaaaga
     6001 aaatattcaa atctgtaaaa ctaatagcaa atgttcctaa aagcggtatg acagtaaata
     6061 ataataaaac taaaaaagat ggagttaaaa acaaatacgc agcacgatca ggacgattcc
     6121 aatatttggt gaatccgttc atatagtcca cctcatcttt cattttgata aaatatacaa
     6181 tcttggttta aacgaatctg aataagatat ccgaacaatc gctgtgattt ttgcaataac
     6241 acctatctgc aaatactagg tcgatcattc agatatctgt atcttgccaa cagtctatcc
     6301 tgtataaagt tctgtactat ttttgcagta attgattgat ggcatcatct gcatcgttca
     6361 tgagtgtctc aggatcttct cctgctaata atttttcaat cagtggattg atgatatcgt
     6421 tattgatcgc agagatttgg gtaaaacctg gtaaatatgg ctctgcatag tccatttgtt
     6481 tcgataattc agatacaata ggatcatttt ttacttcttc atcttttgct actgaatcca
     6541 agtaggcagg aaaaccattt tcgatactcc attttttacc aatttcagtt gtattccaat
     6601 atttcaaaaa ctcatagaca gcttcttttt tattattatc tgtacttgcc ggaatggcat
     6661 atcctacgcc atctaaaata gctgtttttt tgtctgaatc actcccttgt ggtaaggtag
     6721 tcacattata atcaatctcg tttgctttca atccgttatt taaccatggc ccattgattt
     6781 caatggctag ttgaccagca ttcattaaat tgtccatttc ggcgccagaa atcgattctg
     6841 gaccagtctt gtctacatgg atcatttttt ggatcgcttt taacgtttta aggttctcag
     6901 gagacgcaaa ttcagactta gtataatcat cgttcactaa tttaccgcca tttgctaaca
     6961 tccagttata taacggtgca gttccatcct tattgaagac aaaacctgat acatttttac
     7021 tactatcagt taattttggt gccatttctg tcaattcttc ccaagtagta ggtggttgtt
     7081 cagtatctaa tccagcttgt ttaaacaaat cattattcca atacatatac atgccttgta
     7141 cctgcatggg gacaaaatac tgtttacctt ctacttcacc taattcaata gccgtttctt
     7201 taaaatcaga tttatctact ccatcatatt cccagaaatc gtctaatggt tgtaccgctc
     7261 cgttttctac atactgtgca aaatctggat aatttaatgc aataaagtca ggtgcttttt
     7321 tagaagaaat cgctgtagga agtttttcat tcaaattggc ccaagtcatg atatccattt
     7381 caattttgat gtttttatca tttgttttat taaattcatt aacgatttct tgcaatattt
     7441 caccatcaga tgcagtaaac ccattccaaa aagtcaaagt cacttcttta tcagaatcag
     7501 attctgctga acttccgcca ccgcaagctc ctaaaatacc tgttgcagct accaacaaag
     7561 agccaattaa ccatttttta cctttcattt tttgttcctc cttcatctga taaacgacat
     7621 ctgaccacat tccaagatag cggtttcaat aaacaattga ttttcccatc ttcagctttt
     7681 acatttgttg aatgacttgg ttttacttgt tcacttttag gaagattagt tgcttttgta
     7741 tcaaatccac tcatttcaga aaaatcaatc actccctcta atgataattc agacaagtga
     7801 acagttagat tcatttcttc ttctccacgg ttaactgcaa atatgacaag ttctttgtca
     7861 tttattacgg ctatactttc taaatacggc acttcttcaa aatcttccgt cgcatagttt
     7921 gctgaacgaa tttgtgggct taacacctct ccttgtccat acacagcagc ttgcataaat
     7981 ggataataga tcgtctgtct ccaagcttct ccgtttgttt ctgtcctgat tggtgcaata
     8041 acatttacca attgagcaag acaagcaatc tttactctat ctgcatgttt caataaagtg
     8101 attaatagac agcctaatag taatgcatct tcaaaattat agttatcttc tagtaaagga
     8161 ggagcaatct gccaaggcgc tgcttgctca tcttgttccg ctgaatgata ccaaatattc
     8221 cattcatcaa aggacaggtt gatctgcttc ttactatgtt tcttcgcctt gactgtatcg
     8281 cacatcgctg tcacttcaga aatgaaccga tccatgtcta aggaacgtgc aagataatga
     8341 gaaagatcat ttttttggtt gccataatat tgatgcagag acaaataatc tatttgatcg
     8401 tacgcttgat caagaactgt taactcccaa tcaccaaatg taggcatttg ccgattcgaa
     8461 cttccgcata aaaccacctc aattgaatca tctacgagtt tcatcgcctt agccgtttcc
     8521 tctgctaacc gtccatattc ttccgctgtt ttatgtccaa tctgccaagg accgtccatt
     8581 tcattcccta aacaccatgt cttaattcca aaagggctct cttgaccatt cgaacgcctt
     8641 aaatcactcc aataagttcc tttaggaaaa ttacaatatt cgactaaatt acgagctgca
     8701 tctactccac gagtacccag attgacagcc atattgattt cggcccccac atgctgacac
     8761 cagttataaa attcatgaat cccaacttca ttcgtttcaa ttgtccgcca agctaaatcc
     8821 aaccttctag gtctatttgc tatgggccca atactgtctt cccaacgaaa tcctgacaca
     8881 aaatttccac caggatatct aatcatggga atgttcaatt cttttaccaa gtgaatgacg
     8941 tcttggcgaa atccactttt attagctgat ggatgatctg gctgataaat cccttcatat
     9001 actgctcgtc ccatatgttc aataaatgaa ctgtacaacc gcggatcaac tttgcctaca
     9061 cgcttgacac tactaatcaa cattgtggct tccataactg acctccttta atttatacat
     9121 gaataataca cctcgttgaa agcgttgtca ataatataaa tgttaaactt acttattttt
     9181 tatcgatttt aattaaccaa aatattaata atgattaaaa caaataattt aatataactt
     9241 ttattaacaa atatgttaat atataagaaa aaccttttaa gaggtgatat atatgcaact
     9301 acaaataaac gaagaatcct taccggtata tgaagcatta gctagtaaaa caaggatcaa
     9361 gatcatccag ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg
     9421 agtgagcagt gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa
     9481 aacagaaaaa gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa tcgatatcag
     9541 ttttccagaa aaaatattca atgcttttga tacgaaagaa acatcgatac ctattggtca
     9601 ctatacaaat tatgcaattg aaccaacatg cggtttagct acgatccatg attttatcgg
     9661 gaaagtggat gaaccacgct attttatgga tcctcgaagg atggatgccc gcattctctg
     9721 gtttaccagc ggatttgtcg agtatcaggc acctaatttt ttaaatacag aggatacatt
     9781 ggagatgttg gaagtttctg tggaaatttc ttctgaattt cctttttcaa atgacaattg
     9841 gccttcagat atcactttca gcctaaatgg tgtggagcta ggaacatgga cgagccctgg
     9901 agattttgct gatatccgcg gtaaatatac gcctgattgg tatccagata atcttaatca
     9961 atacggcttg ctgaagacga tccgaattac caaacacttg acgaatatga atggagaacc
    10021 cttgtcaaac atcacgatca acgacatccc taaagagcaa gacacttggc atttgcgaat
    10081 cgaagtgaaa gacgatgcca aacatgtggg gggctgtact ttatttggta aaggattcgg
    10141 taattacgat caggatatca aattaaaagt ttactacagc tgatattttt cttttctatt
    10201 ttataaaaca aaaacagcca atccctatga agggacggct gtttttgctt tattgttatt
    10261 tccagaaatc atccaaaacg gaaattggca aatgtcgttt atgttgtgtc ttgttccacc
    10321 atgcttcgat tttttcttgt gcttgagcgg gaacttcttt tccttctaga taatcatcaa
    10381 tttcattata tgtgacacct agtgcgactt catctgcaat cattggtttc ccatcttcaa
    10441 gatctgcagt cggaattttt gtgtataatt tttccggtgc attcaatgct tgaagcaatt
    10501 gtttcccttg tcgtttgtcc aaacggaata atggcaaaat gtcggctcca ccatcaccaa
    10561 atttcgtgaa gaatccagta atgttttctg cagcatgatc tgtccctaat acggctcctg
    10621 ctctttctcc agctacggca tattgcgtaa tcatacgttg acgtgctttg atatttcctt
    10681 tgttaaaatc gctgattccc acacctgctt cgcttaatac ttgtacttgt gcatcaacag
    10741 ctggtttgat atttactcgc agactaacat cgggctggat aaagtcaaga gcagcttttg
    10801 catcttcttc atctgcttgt tcaccataag gaagacgaac agcgataaat tgatagtttt
    10861 catcttttgt ttcttcacgc atttcttcca tcgttagttg cgccaaacgg cctgctaaag
    10921 tagaatcttg gccgccgctg atacctagta caaaagtttt aagaaacgga tattttttca
    10981 aatatgcttt cataaagtca atacttttac gtatttcttc ttttggatca attgttggtt
    11041 tgacacctaa ttcattgata atttctgtct gcaggctcat ttattctcct cctatgcttc
    11101 tgtatgaagt tctgctacgc tttttttcat tcgttccatg atagccattt tatggttcca
    11161 gcattcagta gacagatcga ctgggtattt ttgcggattc aagtctcgtt tatactcttc
    11221 ccatagtgaa tctagatttt cttttgtata ttctttgatt tcgtttagtg caggtaattc
    11281 gtaaactctt tttccttcga taaagatatc ttgtaaaact gggcgagctg taaagtctcg
    11341 aacagtttta ttgataaacg tatgcactgg atggaacatg aagatagctt cttcttctcg
    11401 agggtcttca tcccataatg tcacgtagtc tccttcagat ttcccatcaa aatttcgagt
    11461 gatgcgccac acttgttttt tccctggagt agttactttt tctgcattgc ttgataactt
    11521 gatcgtatcc atcatttttc cttcatcatc ttcgatcgat actagcttga atactgcacc
    11581 aagggctggc tgatcatagg ctgtgatcaa ttttgtccca acaccccata catcaatttt
    11641 agctttttgc atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat
    11701 ttttgcttcc gtaaatccgg ctgcatctaa ttgttcccga acacgcttag atatataggc
    11761 catatctcca ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta attctttcgc
    11821 cacgcgaatc gcacttggca cacctgattt caacgtatca taagtgtcga ctaagaacac
    11881 gcagtcttta tgggttttag cgtaggccat aaatgcttca taatcatttc cataagattg
    11941 gactaacgaa tgagcatggg tcccgctagc tggtatccca aatatcttcc cagcacgtac
    12001 attgcttgtt gcatctgctc cgccgatata tgccgctcta gttccccaga ctgctgcatc
    12061 cagttcttgt gcccgtctag ttccaaattc caatagtgga tcttctccga tgacagattt
    12121 gatacgagct gcttttgtcg cgatcaacgt ttggaaattg accatattca acagtgctgt
    12181 ttctactaat tggcagtgcg ctaaaggccc ttccacttgg attaatggtt catttgcaaa
    12241 aactagttct ccttcacgag cagaacgaac tgttgcttta aattcaaaat tttgtaaata
    12301 ctccaaaaaa ccttcaggat agacttctaa tccacgcaag taagcaatat cggaatcatt
    12361 gaacgtcaga ttctctaaat aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc
    12421 gtggttaaaa ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc
    12481 taattcccag tatgtctgca tcatattgat ttgatataga tccgtatgca gcgtcaaact
    12541 gtcgtctggg taagtattat tcatcttgta ttactcctct tggcaagttt tcatttccta
    12601 cccattataa caaaaaaaat taataggaaa agaaatcttc cattttttct gaaaaagcac
    12661 atgttttacg gatatttctt gctaaaaatg aactccatct gctaaaacta tcctttctta
    12721 ttggtattta acctaaacga gaaagaatac ttattttatt caatgtatcc gagacacgaa
    12781 gaaattagcg ttttctattt ttcttacttg ataaaataag tagattttcc tactacaatc
    12841 gtagccgtta agataccgat gattgaacaa atataaccaa atgtctctac aacataatca
    12901 atggcaaaag tttttgaagc aataatacgt ctttcctttt ttgcttctta tccccaaaga
    12961 aacggatata acttattatg tattgaattt ttttggaaag gcgaattatg acaagatata
    13021 ccttgcgttg aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa
    13081 gaaagcagct gtcaatagaa tggaaacaaa aatggcgaga catcaatcag tctcgccttg
    13141 ttttttattg tatacattag atatggagca ggttaccata aagatgatta ttagtcgtta
    13201 ttaaatagaa tccttcgtat ttttttgttc tatcactaca atatctttat ttggacgttt
    13261 gaattgtatt ccggcacgtt ctagctcctg tttcactcct acatggactt tgacatcttc
    13321 tcgtgtcatt tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg tgattttttt
    13381 cttcgtattg atcatcacga cttgcttttt atcatatgtg tctaccaata gcagttgtgt
    13441 tcgatctaac gaaaataatt tgtaataatt aatagccgta ttcccaaaaa agaattccac
    13501 tactttagga taaaatgtct taatatttaa attactattc acaggtgttt cgattagctt
    13561 catctcattc ctcttttcta tatagtcgat ttagatccaa tcttaagtta tgttaccatc
    13621 tttcctagca ttaaggaatc ggttacaatt gaacctttaa agacaaaagg atttaccttt
    13681 tatcacttaa tagtataaca taatttcgtg agaaaaaaat acaatttttt cttgaccatc
    13741 tcgttacgta aaggattatg cttacatttg tcaggaggaa aaataatgga gtacacaata
    13801 aagaaaatgt cagagatatc aggagtcagt ccgagaacct tgcgctttta tgatgagatt
    13861 ggactgctaa aaccagctag gatcaattct tcaggttatc ggatttatgg caaaaaagaa
    13921 gtcgatcgtc tgcaacacat cttattttat cgaacaatgg cattcaagtt ggatgatatc
    13981 caagaggtct tagacaatcc atcttttgac caccagaaag ctttaatcaa gcatcagcaa
    14041 atgttgctgg aaaaacgggc acaaatcgat accctcttga caactgtcca acagacactt
    14101 gatatgtacg aaggaggaag aaaaatgagt gacaaagaaa aatttgaagg gtttaaacaa
    14161 caaaagttga aagaaaatga agaatcctat ggacaagaga ttcgacaaaa atacggaaaa
    14221 aaaaaggtta cagcatccaa tgaaaaatgg atgaatatgg ataaagaaac gtatgaagaa
    14281 atgcaaactg ttgaaaagca gatgttattt gatctcactc tttacttaaa agaaccagaa
    14341 gatgaaagct tagcagaccg tatcttccaa gctcataaga agtggttctc ttacagctgg
    14401 cctgaatatc agccaaaagc gcataaagga ctaggaatga tgtacgaagc cgatgaacgg
    14461 ttcactgcat attatgacga gcgaagtggt catggtgcag caaaagcttt aaatgaaatt
    14521 atccaaaaaa aggcgtaatc ttttacatga tccaacaaaa aagtacacgt aaaatgacct
    14581 aataacaaaa cagcagacac ccaagttaga tttttatgct aacttttggt gtctgctcca
    14641 ttctttcaca agttttttca tcctacttta aatatattca tcgttgaata aacgtataag
    14701 gtattttcac tttatccttt ttttctgtaa gaaacaattg aaatgccatt tctccgcaag
    14761 cttccagatg gtggtcgatg gttgggaaac caagaagctc gctgatcagc aaattatcgc
    14821 gtccaatcac cactggtgtc ttatcttgca gatagctttg caagatgcct gctgcaacca
    14881 tatccgaatt tgtaaagaca ccatcaactc caagctcttt aaaaaagaag cctgcttgtt
    14941 ttcctttctc tgcatctata caatcccaaa aaacaagtga ttcatcaaaa gatggaaaaa
    15001 gttgatgaca tagctgcaaa gtgatttttg agttataact tagcctgcca cttcgcccca
    15061 acgtgatgcc caattgcttt ataccttgct gcttcaaata agtaagacct tctgtaattg
    15121 attgttctcg atcaataaag acacagcttg cctgttttcc cttgatttct tcacaaaaaa
    15181 ctagaggtcc atatttttga tatgccaata aatcatctag tgtattggca cgagacgtaa
    15241 tgatcaatcc atcgaaagct ttagctgcaa attcttcaag ataccttcgc tctttttctt
    15301 tatgatagcc cgtcggtaaa agtgtcactt tgtaatcata atcaaacgct gcttcaatga
    15361 tcccactgat caactgatca aaatacggat gattggtata aggcagaatc actccaatgc
    15421 ttttcgtttt tccataactt aactgtctgg catttgcatt agggatataa tcgacttctt
    15481 tcatgaccgc aagtatttct ttcctttttt cctcatctac atatggatgt ccattgatta
    15541 cacgtgaaat agtagaaact gaataacctg tgagctttgc gatatctcga atgtttgcca
    15601 ctgctgcttc gccacctata caaaaagatt ttcttgtaaa tagtctacca cagctggacc
    15661 gcattcaaaa aactgataca tttttccttc atgcgaaaaa ctgatccgct gcccgcccca
    15721 atatatatct tttgtcaaat tttcgatttc attcattggt atttctttta ctatattcct
    15781 attcaaccct tgaaaactgt acaacgttaa tacggatccc ttgaactcga caaacgcata
    15841 aaaataattg ttcgttgtgc catctgttat ctctacataa gtattcattg caagccctcc
    15901 ttttgatact ttgagtatag tgtatgaaac gcgtctcata gcaaggagtt tgttttgcca
    15961 aagaaaagtt ttattcctct ttcattatta ccaaagtctc ttattttaaa ttgaatacat
    16021 tataatgata ctgtaaatat atctttatta ttagtgaatg
//
LOCUS       DACEOE010000054        15990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEOE010000054 DACEOE010000000
VERSION     DACEOE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.056"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..385)
                     /locus_tag="IW774_001390"
     CDS             complement(<1..385)
                     /locus_tag="IW774_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1153744.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDI"
     gene            complement(515..1225)
                     /gene="budA"
                     /locus_tag="IW774_001391"
     CDS             complement(515..1225)
                     /gene="budA"
                     /locus_tag="IW774_001391"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1153745.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1238..2902)
                     /gene="alsS"
                     /locus_tag="IW774_001392"
     CDS             complement(1238..2902)
                     /gene="alsS"
                     /locus_tag="IW774_001392"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1153746.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3107..3769)
                     /locus_tag="IW774_001393"
     CDS             complement(3107..3769)
                     /locus_tag="IW774_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1153747.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3779..4594)
                     /locus_tag="IW774_001394"
     CDS             complement(3779..4594)
                     /locus_tag="IW774_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1153748.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4856..5299)
                     /locus_tag="IW774_001395"
     CDS             complement(4856..5299)
                     /locus_tag="IW774_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1153749.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5330..5443)
                     /locus_tag="IW774_001396"
     CDS             complement(5330..5443)
                     /locus_tag="IW774_001396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1153750.1"
                     /translation="MEADNSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(5433..5771)
                     /locus_tag="IW774_001397"
     CDS             complement(5433..5771)
                     /locus_tag="IW774_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1153751.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5759..6136)
                     /locus_tag="IW774_001398"
     CDS             complement(5759..6136)
                     /locus_tag="IW774_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1153752.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6360..7553
                     /locus_tag="IW774_001399"
     CDS             6360..7553
                     /locus_tag="IW774_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1153753.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7719..8141)
                     /locus_tag="IW774_001400"
     CDS             complement(7719..8141)
                     /locus_tag="IW774_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1153754.1"
                     /translation="MQTFLTKASQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8731..9186
                     /locus_tag="IW774_001401"
     CDS             8731..9186
                     /locus_tag="IW774_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153755.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(9348..10520)
                     /locus_tag="IW774_001402"
     CDS             complement(9348..10520)
                     /locus_tag="IW774_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1153756.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10726..12155)
                     /locus_tag="IW774_001403"
                     /pseudo
     CDS             complement(10726..12155)
                     /locus_tag="IW774_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(12152..13654)
                     /locus_tag="IW774_001404"
                     /pseudo
     CDS             complement(12152..13654)
                     /locus_tag="IW774_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(13651..15978)
                     /locus_tag="IW774_001405"
     CDS             complement(13651..15978)
                     /locus_tag="IW774_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1153757.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4552 a         3207 c         2847 g         5384 t
ORIGIN      
        1 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
       61 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
      121 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
      181 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
      241 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
      301 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
      361 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
      421 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
      481 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
      541 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
      601 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
      661 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
      721 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
      781 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
      841 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
      901 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
      961 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     1021 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     1081 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     1141 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     1201 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     1261 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     1321 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     1381 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     1441 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     1501 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     1561 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     1621 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     1681 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     1741 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     1801 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     1861 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     1921 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     1981 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     2041 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     2101 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     2161 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     2221 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     2281 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     2341 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     2401 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     2461 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     2521 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     2581 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     2641 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     2701 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     2761 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     2821 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     2881 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     2941 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     3001 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     3061 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     3121 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     3181 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     3241 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     3301 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     3361 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     3421 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     3481 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     3541 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     3601 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     3661 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     3721 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     3781 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     3841 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     3901 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     3961 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     4021 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     4081 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     4141 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     4201 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     4261 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     4321 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     4381 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     4441 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     4501 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     4561 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     4621 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     4681 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     4741 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     4801 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     4861 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     4921 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     4981 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     5041 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     5101 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     5161 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     5221 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     5281 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     5341 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     5401 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     5461 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     5521 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     5581 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     5641 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     5701 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     5761 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     5821 gtaaacgttc caaacattct tgcatttcag caattttttt cgtcaaaagt tcactttctt
     5881 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     5941 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     6001 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     6061 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     6121 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     6181 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     6241 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     6301 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     6361 tgacaacgta tcagcgaaag ttattattat cgacatcggc gggaattgct ttagagaata
     6421 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     6481 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     6541 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     6601 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     6661 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     6721 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     6781 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     6841 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     6901 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     6961 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     7021 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     7081 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     7141 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     7201 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     7261 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     7321 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     7381 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     7441 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     7501 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     7561 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     7621 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     7681 gctttttcaa ctgctaattt ccttctatct ggcggctact attctttgac gtccgtcatt
     7741 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     7801 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     7861 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     7921 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     7981 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     8041 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     8101 ttaattttct tttgagaagc ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     8161 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     8221 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     8281 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     8341 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa aacgggatta
     8401 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     8461 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agtgaaaaaa
     8521 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
     8581 actactcaaa gcacaagttc agctcaggct tccagcagca gtaccacgat ctccgaaagt
     8641 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga agagaaacaa
     8701 aaagcggaaa aagctgcact agaaaaaaaa gtgacagaag cggaatctgc tttgtcacaa
     8761 gctgaagcaa atcctacaaa agaaaactat gcaactgctt cagcagctat ccaagcgcta
     8821 cctgaaaaca aacaagagtt gacaagccgt cttgctgcaa ttgattccac aatcaaagca
     8881 aaagaagcag aagaagaacg gaaaaagcaa gaagaaattg cacgacaaca agcagaacaa
     8941 gcgcaacaag aagcaatcgc acagcaacaa gcagaacagg cgcgacagca agcagcagct
     9001 gaacaggcct ctcagcaggc acaagccgca gctcctgagc aaaataatga acaaactgtc
     9061 tatgtcacac cgaccggttc aaaataccat acacataaat gtggaaatgg tacgtatacc
     9121 cctgccactt taagcgaagc tcaagctcgt ggtttaaccc cttgctccaa atgttatgga
     9181 aactaattca aaagttatac tataataata aaattcttct gaaacataaa aaaatagaag
     9241 ctggcgttcc agatagtaaa ccaaaaatag cagtttacac gtccatttcg tcagcttctc
     9301 tcttttttca tttagtctgt tccctctgct tttttggaca tcttcattca ttctttctca
     9361 atcaaatagt acacctcttt ttcttttcgc agtttatatc ttcctttttt caatttcatt
     9421 gaaaactgtt tcgaatgttt atgttttaca tggattttaa gcaggagctc ctcatgttgc
     9481 aagcgatagt tcctgccttt taaatcagaa aatctcacta gtccgtattc atcagaagct
     9541 ttcacaaatg gaactttttc tctagtaata tagtgtttgc cgttgagcgt ttttacagaa
     9601 aagaccattc cttcgattgg tcgtccgttt gtgttcttta cataaaaaga taaaggatag
     9661 tttcgcttag cgatggccaa agaatctaaa taagcgatga gtagccaaac taagccgatt
     9721 gatccaagta gtataccgat aaaccaaata aaaaatttcc attttttcca ttgatcaatt
     9781 ccttggatcg ctttttttgc ctcttttgct tgataaggaa tacgatgccc cgtcacaagc
     9841 agtcgatgtg tattgaccat ataaggagta caggtaagca aagtcacgag atctctgcct
     9901 ttttggatac ccaaagaatc tacttcatca ggtaagacca cttggatttt ttctacttga
     9961 tacgccaatg tctttccatt gatctggatg aaaaactggt ctcctttttt caacttaggc
    10021 aaatctgtaa ataattttgc ttctgggagt ccacgatgcc cagaaagaac ggcatgggtg
    10081 tttttcccgc cagtcggata agaactttct tccaataatg acgttccttt ttgcaaaaat
    10141 atttccgttg tctgatcgaa tataggtaag ctgacattga tttttgggat ttcgatgact
    10201 ccaagcatat gttccataaa aaaggcatta ctaggtaaat cttttaatgc ttttgcatca
    10261 actgcttggt tgaaactgcc tatccctggt gatacgtttt gctctgccaa ctgttggttt
    10321 ttctttgtca ttttttcctg ctgttttttt atttcctctg aattcttatt cgatgcttgc
    10381 ttttgataat ggatgatcat ttgctgggca agaaaatcat tcagtgaatc ttctacaaaa
    10441 ggataagaaa agatcccgat cccaacgact aaaatgattg ccatgaagat gtccagtatt
    10501 cttattctgc ttttattcat catgtccacc tattttctaa tatgtatatg aagaggttgt
    10561 gaaatcagcg ccttgacctg agtattcgct caacaaacgg caaaatagtt cctcctattt
    10621 tgccgtttgt tgagcgaata ccaagagacc gaccacttgt cacaaccttt ctccccattt
    10681 gaatcactat cgtttattta tcgtatgatt tttgtttcca atagtttaag cttctgtttc
    10741 ttgatggcgt tttttgaagt agatcactgc tcctccaact aaagcagtac ctaccaagat
    10801 gaatacgatg atccccatac caccagttga aggcaagatt cctttaggtt ggttatcgat
    10861 cgttgtttca tcagaagttg ttggatcatc atctactgta aattcagtgt acgcagcttc
    10921 attcttaggt aatgcgtagc cttctggtgc ttttgtttct tgaaggcagt atgccccttg
    10981 tttcagccct tcgattgaaa atttcccttc tgaatcagaa atcaatttca cttcttccca
    11041 gtcagaacct gtgacttcat ctgaccaagc atatgggtat tctcctgagg caagatttgc
    11101 tatttttttc attaatttaa cagttggatc ttttgctctt agaatagaaa attctgcatc
    11161 ggctaatttt tcttgcgtgt tcacatcttg tttgatgaag tggaaatcac cgtagttttg
    11221 gacattttct acagtaatcg tgttatccgt agttgtattt tcccgattga ctacaccgtt
    11281 tggattccca agattggctt catctgcgcc atggatgata ccggttgttt gttgcaattt
    11341 tccgtttttt actataaaag tagtttcttt ttgtggattg gttaattgat aaccatctgg
    11401 tgcttttgtt tcaactaaag tataattgcc gtctattaat ccattgacac tgatggatcc
    11461 ttcagtatca gttgtgaatg tcaacgctga ctcagcagga tcatagagag cgacaccagt
    11521 gttttcatca aaaccggtca cgaattgatt ctctgaattt ttgatctgga attgcgcccc
    11581 tgctaactta tctacccctg aaaagttccc agctttctgg attttgatat tgctttcttt
    11641 gatgacattt ttagcatata ggtgaatcgt gtctaaaggt ttgtcagggt ctgatgaatt
    11701 cgggttataa aagggtaagc ttaacacgat atttggcgac gtttcgacac cgggctttgg
    11761 tgtttctaca aagacgtata cagcatcttt cccatcagat ttacctggaa gagtcaggat
    11821 agctaatcct tcaccagaag tcgttacact tgcgaagggt gtagcgtctg ctggaactgc
    11881 tgtgtcttta cttggatagt acgtagcagt gatgtcatat acgttgaatt caacgccgtt
    11941 caacggttcg ctattcggaa atgtcaattc cgttccgttg tttatttgat tttctggtgc
    12001 tgaagtgaat ttcaatttat gcaaattaac cgtaacattt tctggtgtag gtgatacggc
    12061 ctagaaacct ctgcagttcc taggccgaga acgaaaggaa gcagcatgat agcagttcct
    12121 aaaaacgtcc atatttttgt tttgatgttt ttcattttat ttccctccta ttgtttcctt
    12181 tttgttgttt tttataaaac caagctgtcc ctgttgaaag gactagcaac aaccctaaaa
    12241 ttgagaaacc tccaagcatc tcttcgcttg tttttggaaa gcttcctcca tttttttcat
    12301 ttgatggacc atttgtctgc atctgaactg gcgtttttcc attattcgtc ggatttgctt
    12361 ttggttcgtt gatgatttcc aatggtatag gatgaagcgg atcataactg cctttttcaa
    12421 cttcaaagag aatggttgat tgactcaata tatatccttt aggcggttta atctccacta
    12481 gttcgtaact tccaaaagac aatccatgga cagaaaatgt tccatcttta ctggaagtaa
    12541 aaatcgttgc tcttgaatca tctttggcta cccaatgatt tatcccgttt tcttgcgcta
    12601 agtattggtc attttttttg actagaaatt gagcatctgc tagtttttgt ttcgtttgcc
    12661 gatccacttt gacaaattgt ttgcctcctg tttggatggc aagatgtgtt tctactgttt
    12721 gattgtcagt cgtcaaacgt acatcgttat caaatgtcgt tttttcactg ttttctttca
    12781 atgtcatttg gtaacggatc gttaattttt tcccagcaaa cgaactaaga tcttttccag
    12841 aatctgataa gacaaaacca tgttttgttt tttgtgtgtg aaatgtatga atcttgtctc
    12901 caccaatcaa aaaatcgatc gtttctggtt ctagccataa ctgaggatca gctgtatctt
    12961 tgatcgtata ggttcctatc tcgttgatgt tctcaggaat tgttacggtg atgagataag
    13021 gaactttatc cccatctgca aaatctgtct gcttattctc tactgttttt gttaatgtga
    13081 gtgcagcagg tattttttgt tcgctctttg gatacaagtg gatggtcgtc aatttatgat
    13141 tagatgaatc tgtcacagga agcacaacga caaatggtgc tgatttttct tttacttaat
    13201 ctgaagtctt tatttccgca aatcggtaaa cagcgtcacg accttgctca tctttgtccg
    13261 atgcactaaa agaagcaatg ccctcttcct cgtctactgt ttttgtcaca ttttctgcca
    13321 gtattttttc actatcactt ttttgtgcta gttttctttg tgcgtcttcc acactacttc
    13381 cttctgaccg cagcttgtaa aattcatccg agacatcata tatagcgaaa gtaacgccgt
    13441 tcaacccttt tactccttga aagttatccg ggatatttat tccgtcgttt tgactgatac
    13501 ttggcatctt atcgctttcg aacattcgat tatgcaaagt aaatgtaata ggtgattctc
    13561 tagctgacac agtcggagaa cctataaaaa agaccagtag gataaaaaag agaacagcaa
    13621 atatatccac acgtacattt tttgtttttt tcatcgctgt tcacctgctt ttttcttacg
    13681 atggataaaa taaaagaagc cagaaaaaat aaagatcagt cctaaaaaga caaagggcag
    13741 tagacctatc ccacctgtcg aaggaagcaa gagagtcgga ttgatcgtca acttagcggt
    13801 atctgttgcg ataatcgtat aattattgtt tacacttcct tcgcctaaac taatcgttac
    13861 tatttgtgat ccaatatctg tccggctgtt aactgttagg gtataatcca atgattcgcc
    13921 taagacaaca ccggtaacgt ggagatttcc aaccgaaatc gagtgacttt gggcatcata
    13981 attaaaggca gcgtcatcga cagtaatcac tgcgttagct ttatagatgg tataagttcc
    14041 tgatgtgttg gctatatcag taaatgacaa cttgggattt gctgctttga ctttgtctat
    14101 tccgctattc gttagtttaa ttgtatagct gccagcatta accatatttt ttgctgattg
    14161 tgcatctgct gtgtaatcca tactgctaag tgccacttgt actggagtaa gcgaactagt
    14221 gacaccctct gctagcttga catggattga cgggacacct gcttttccat catatgtctt
    14281 actgccactg agctctaccg caccgctgat ttgcttaatc gtatagacat cagatgtctg
    14341 cacgatagta aataaatagt ttgttctggc agctagttta gctagtccgc tttttgtcaa
    14401 ttgccaagac gtataggttc caatttcagt aggcgcagtt acttcaactc ccttttcatt
    14461 catcgctttg aaatcagttg gattccatgt gatttgatca atactgagat cattgtttaa
    14521 aagggatgtt gattctgttt tatcattaaa ggcatattta acgatcggta caacagaaag
    14581 accatcatag tacttactgc catttgcatt gattgatact gttgcatttg tcttataata
    14641 taggatgact tcagaaatct gttttccatt tgcgtccttt acgattacat ttccatttgt
    14701 atctaaacta gctaaaacac tcattgctga ttggttcttt gtttctgtcg ctgaaacagc
    14761 tgtaaaggta tatccgttga tagtaggcgt attagtcgtc aggtcttgtg cagcacttcc
    14821 gtaagtaaga ttcattattt tagaaggagc aatcatctgt ccaaaatagt cttgatactt
    14881 aatagtgatt ggtgtttgtt tgcctgtata ggtcaccgta tcgacgttac tttctagggc
    14941 tgtgaaagaa ccgtttgttt gtgcaaaaga atagcccgtt actgctgttg gagtgataac
    15001 cactttgtca cccacatgga ttccagataa tgtagtcccg tacgttccga tttttgctgc
    15061 tccaccattg actgtataag aaaaagttcc tgtccgattc gcattgacct gaaccgttgc
    15121 tgcttgatag gttggaaaat ttgaaacatt tcccttacca tcaacgatct gcccattttt
    15181 gatcgagtac cccgtgaatg gaatatcatt tgctaactgt ataccgattg aaaaagtact
    15241 catacctcct ccggcaggaa gccctcccca tgttgagggt gtaaaagaat aacttttatc
    15301 cgcattgggt gtaactgaat cgatcgtatt tccagcggta gccaccccca tatagatact
    15361 ttttgggaat gtgatagtag taccatttac tgtggagcca ataaacagat tatggatatc
    15421 tggacggtta tcaataaaag cgattgcttt ggttacgcct tgtaacgcag ttttatccgc
    15481 actcatcgat ccgttaaccg caacaatgac tccatctttg gcatatatca ctgtatatcc
    15541 atctggtatg gtatgtccgt tgaaattcgc agtggccgca acagtactaa atgtctggct
    15601 tctacctgtt tctgaataag ctttctcccc tgctgctttt gctgctgtga tttctttttc
    15661 tgtcggatca atcagttgag tcgaggatgt ggttgaggtt gtctcgttgt tgctagactg
    15721 attggtgctt tctatggact gtatttcaga atagggtgga ctatttgtct cctctgtcgt
    15781 ttggttagca aaatttgacg tttctgtata aacgtttaca taaatacttg ataagctccc
    15841 taagaaagta aaaatcaaga tgatcgaaat cataatggga tgtaagtatt ttcttttaga
    15901 tacagctttg agtctagaaa caaaatgttt ttgacatata cgttggattt tattttgagt
    15961 tttcttaatc attttcatcc ctcctctcaa
//
LOCUS       DACEOE010000055        15871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEOE010000055 DACEOE010000000
VERSION     DACEOE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.057"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW774_001406"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW774_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1153758.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IW774_001407"
     CDS             808..1242
                     /locus_tag="IW774_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1153759.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IW774_001408"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IW774_001408"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1153760.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IW774_001409"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IW774_001409"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1153761.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="IW774_001410"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="IW774_001410"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1153762.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="IW774_001411"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="IW774_001411"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1153763.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="IW774_001412"
     CDS             9513..10439
                     /locus_tag="IW774_001412"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1153764.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="IW774_001413"
     CDS             10621..11514
                     /locus_tag="IW774_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1153765.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="IW774_001414"
     CDS             11648..11773
                     /locus_tag="IW774_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1153766.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="IW774_001415"
     CDS             complement(11907..12539)
                     /locus_tag="IW774_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153767.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="IW774_001416"
     CDS             complement(12687..13229)
                     /locus_tag="IW774_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1153768.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="IW774_001417"
     CDS             13364..13753
                     /locus_tag="IW774_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153769.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="IW774_001418"
     CDS             13757..14521
                     /locus_tag="IW774_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1153770.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15586
                     /locus_tag="IW774_001419"
                     /pseudo
     CDS             14650..15586
                     /locus_tag="IW774_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
BASE COUNT         5588 a         2672 c         3307 g         4304 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc
    15181 aagggaaact ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg
    15241 gtttccaaat cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac
    15301 agataaaact tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat
    15361 tataaatgct ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt
    15421 tttagaccaa atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg
    15481 tgaagtatgt ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta
    15541 tcgtcgtttt tttcctgaac tagttatcag agagacaacg aattaagtct tattactata
    15601 tatatattta tgaaattcat cgctattttc ttaaaataat cagcgcattg ctactgaaaa
    15661 caatgaatct gtgctattat tgagaaaagt tccatttttg agaaggatgg tttgaatgaa
    15721 agaaatcaga cgaaaacatt tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt
    15781 aaccaacgac aagaagcgag tattatttga cggacaagaa gatcttttga ctgaacagaa
    15841 tcagtccccg tttgaacaga tgaaagaatt t
//
LOCUS       DACEOE010000056        15563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEOE010000056 DACEOE010000000
VERSION     DACEOE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.058"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(119..499)
                     /gene="yajC"
                     /locus_tag="IW774_001420"
     CDS             complement(119..499)
                     /gene="yajC"
                     /locus_tag="IW774_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1153771.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(596..1741)
                     /gene="tgt"
                     /locus_tag="IW774_001421"
     CDS             complement(596..1741)
                     /gene="tgt"
                     /locus_tag="IW774_001421"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1153772.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1959..2657
                     /locus_tag="IW774_001422"
     CDS             1959..2657
                     /locus_tag="IW774_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1153773.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2825..5464)
                     /locus_tag="IW774_001423"
     CDS             complement(2825..5464)
                     /locus_tag="IW774_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1153774.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6083..7114)
                     /gene="queA"
                     /locus_tag="IW774_001424"
     CDS             complement(6083..7114)
                     /gene="queA"
                     /locus_tag="IW774_001424"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1153775.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7307..8482
                     /locus_tag="IW774_001425"
     CDS             7307..8482
                     /locus_tag="IW774_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1153776.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8486..9127
                     /locus_tag="IW774_001426"
     CDS             8486..9127
                     /locus_tag="IW774_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1153777.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9124..10041
                     /locus_tag="IW774_001427"
     CDS             9124..10041
                     /locus_tag="IW774_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1153778.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10038..10709
                     /locus_tag="IW774_001428"
     CDS             10038..10709
                     /locus_tag="IW774_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1153779.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11250..11738)
                     /locus_tag="IW774_001429"
     CDS             complement(11250..11738)
                     /locus_tag="IW774_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ1153780.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11939..12205)
                     /locus_tag="IW774_001430"
     CDS             complement(11939..12205)
                     /locus_tag="IW774_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1153781.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12205..12468)
                     /locus_tag="IW774_001431"
     CDS             complement(12205..12468)
                     /locus_tag="IW774_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1153782.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12461..12733)
                     /locus_tag="IW774_001432"
     CDS             complement(12461..12733)
                     /locus_tag="IW774_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153783.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12944..13129
                     /locus_tag="IW774_001433"
     CDS             12944..13129
                     /locus_tag="IW774_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1153784.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13271..15094)
                     /locus_tag="IW774_001434"
     CDS             complement(13271..15094)
                     /locus_tag="IW774_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1153785.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4534 a         3319 c         2917 g         4793 t
ORIGIN      
        1 acacccgatt attcaatatt gagcaatata tcccatgctc ttatctcttt cttttttgaa
       61 taaaaaatga ggtaggacat ctttctgccc tacctcgtct ttaaataagc tttgatactt
      121 atttttcttc cttctcttct gtctcttcca gcgtttctgt tgtttcagtt tttacttcaa
      181 ctgtctcagc tggttcttct gttgtttcaa cagtagtcgt tgcatcattt gttacgacag
      241 tccctggttt gaccgttttg attgctgcac gatcgtactc aaggaagatc ccttcgcagt
      301 ctaaaacaac tgtccgtttg tcattgtcga tttctgaaac aactccatgt aatccgccga
      361 tcgttacgac tgaatcacct ggtttcatgc tatctaacaa gttttgacgt tcttgctgtt
      421 gtttcttttg cgaacgtgac ataaaaaacc acattgctaa tagcgcgata aacatcacaa
      481 gcattggtaa tgttcccatt tttttcacct cactaattct gtatctacaa aaaacacttt
      541 agcagaaaaa tgctgataac actagttttt tacttaattt ttaaaagatt ttcctttaga
      601 agctttttgc gttttcttta ttgaacccgt attcttcaaa aaaggcttct ctaaattcaa
      661 gcaaattatc atccatgatt gcttgacgta cttgtttcat tagattcaac aagaagtata
      721 aattgtggta agaagtcaaa cgaatcccaa atgtttcatc acatttgatc aaatggcgga
      781 tataggcacg tgtatagttt ttacatgtat aacaatcaca tttctcatca attggacgga
      841 aatcatgggc atatttcgca tttttcacta ctagacggcc ttgagaagtc atacatgtgc
      901 catttcgagc gattcgagta ggcaagacac agtcaaacat gtctactcca cggataacac
      961 catcgatcaa tgaatcagca gcaccgacac ccatcaagta acgtggtttg ttttcgggga
     1021 tcaacggtgt agtgaattct aatacacgat tcatttctga tttaggttcc ccgacagata
     1081 acccgccgat tgagtaacca gggaaatcca tcccgactag atctttcgcg ctttgacgac
     1141 gaagatcttc aaaccctgct ccttggatga ttccaaataa tccttgacgg tctggattag
     1201 catgagcttt caatcctcgt tctgcccatc gagacgtacg ttctactgat tttttcacgt
     1261 agtcgtaact ttcatcgaat ggtggacatt cgtcaaaact catcatgata tcagagccca
     1321 atttattttg gatgttgatt gctttttctg gtgataaaaa catacgcgaa ccgttcaagt
     1381 gatttttgaa atgcacgcct tcttcttcga tattccgcat atcacttaat gaaaaaactt
     1441 gaaagccgcc tgaatctgtt aaaatcggtt gatcccagtt cataaattta tgcagtccgc
     1501 cagcttcttc tactaagtct tcaccagggc gtagccataa atgatatgta ttacttaaaa
     1561 taacgccagc acccatttct ttcaactctt ctggggacat tgtttttact gtagctaatg
     1621 ttcctactgg catgaacatt ggtgtgggga aagttccgtg tggcgtgatc aattccccta
     1681 aacgcgcgcc agtatgtttt tctttcttga ttaaacgata gcgaatggca ggttgcgtca
     1741 ttcatttccc tactttctat ataaaatatc ttgacaactc ccaccatcat aatcgtttca
     1801 agtgcttttt gcaagttccc tcggcttcct tctactttat ttaatattct tttttttatc
     1861 ctttttttat gaataacaaa aaaaagaaaa acattatgac agaaatattt tttgatacat
     1921 ggtatattgg tgttattaaa gaaaaggagg gaaaaatcat gcaatcttct gcagaattaa
     1981 aacgccaagc aaaagagagt ctaaaaggac gttggggaca agctgtttta ctaaatctaa
     2041 tccctacatt gattggtatt gctactattc ttcttattat gattcctatt atcttgtttg
     2101 ccaccaccat atatgatgga tcagcgagcg tctcagaagc gacaacttca ggaggcggta
     2161 gttcaggagg cggtggcgga attatttcca gcatcattag cgctttgttc atgagcggta
     2221 tttcttggac ttatcttgat attttacgtg gacaaaaaga tcagatcgaa ccattcaaag
     2281 atgcatttcg tggattcaaa ggtttgttta tcggcggagt gatcagctta gctgtgctga
     2341 tctcgatctt tacaacatta tggggtttgc tattagttat tcctggaatc attaaaagct
     2401 attcttattc tcaaagctat tttatttact acgatatcgt ccaagaaaca ggacaaacac
     2461 ctaaagtact ggatacgatc actgcaagtc gaaaattgat ggatggttat aaaggacaac
     2521 ttttctggtt agatctttca tttatcggct ggcatatctt agcattagca actgctggta
     2581 ttggttatct atggctcagc ccatacattt ctgcaacaaa agcagcattt tatgaacaat
     2641 tgccaaaaga tatttaaaag agagtgtgac ttgtgaccga gggggtggca ggagaacacc
     2701 gtttatcgag aggcagtaaa agaaacattc agcccattag caaaaaggag gtgtgacaac
     2761 attcatgtca cacctccttt ttgtatagtt ccagtaaaaa gaaaatcaac ttagaaacag
     2821 ttaatcattc ctcttcttct gcttccggta atcgatggaa cttgatcagt tcattcaccg
     2881 cgattggtac gatagataaa atccctacca agaaccagtg attcaaactg atgtgtacta
     2941 ggccgaatag ttcttgcgta aatggcagca aagcaacagc cgttgtaata gctaatgcca
     3001 gtaacgccat ttctaccaaa gctttattgg tcgctaaatt gactttgaag atcgattggc
     3061 tgctgcgtac attgaacaca tgcagaatcg atgaataagc taagatcaag aatgcaacag
     3121 tttgaccgac atgctgactt gcgtctaccc atgggctgat agtatcgaca taactaccta
     3181 aataataacc aaacaaggtt acgatggtga agacccctgc atttatggca attttttgca
     3241 ataaacctcg agcaaagatc ccttcatttt tcggaatggg tggctgatcc atgatgccgt
     3301 gttccgctgg ctctcttcct aaagcaaatc ctggaagtcc atcagcaact acattgacaa
     3361 acagtagttg cacagctgta aatggtgctc cccatcctaa cagcatcgca atcaaaacaa
     3421 taaagatctc cgaaatatta cagcttagca aaaagttgat agctttacga atattctgat
     3481 aaaccgaacg tccttgactg acagcgtcta cgatcgtcgc aaagttgtca tccgtcaaaa
     3541 tcatatctgc ggcactttgc gccacatctg ttcctgtgat ccccatggca cacccaacgt
     3601 tacttgcttt caacgctggt gcatcattca caccatcccc agtcatcgct acgactgcac
     3661 ccgtacgttg ccaagatttg acgatccgga tcttgtcttc tggcgttacc cgagcataga
     3721 cagagagagc tttgacacgc gcatctaatt cttcgtctga taattgatgc agttctgatc
     3781 ctgaaagtgc ttctgacgga tcattcaaga tacctaattc tttcgctatg gcactggcag
     3841 tcaccacatg atctccagtg atcatgactg ttttgatgcc agcctttttc gctcgcttga
     3901 ttgctccttt actttctgga cgtggtggat cgatcattcc gatcagacca atcaaacgta
     3961 agtctttttc cagtgcttca gaagaaatat cttgcggttc ttcgtcataa gtagcataac
     4021 caacagcaat cactctcagt gcccgtttac caaagcgatc attaacgatc gccgcttgtt
     4081 ctacatcgcc tgaactaaag cgcggagcaa gaacatcgaa tgctcctttg gtgattgaga
     4141 tgtatttttc gcccatttgg tgaactgttg tcatcatttt tcgttcagaa tcaaaaggaa
     4201 gctcgtttac tcgtggatat ttttcttcta attctgcctt tgtatgataa ttctcttcaa
     4261 cagcccttac gatggctact tctgttggat tacctttgat aacaaggtcg tcatcttctt
     4321 gctcgaccgt cacatctgta catagtgcag ccattttcaa tatttccatc gcgctgttcg
     4381 tcattgaatc ttcgatattc gttacttcat cttctttgct ccatacccgg cggacgcgca
     4441 tttcattttg tgtcagcgtt cctgttttgt ctgagcagat aacattagcg gttcctagcg
     4501 tttcaactgc tggcaactgt cggatgatcg catgtttcct cgccatcttc tgcacaccgt
     4561 atgcaagtgt caacgtaaca ataacagtca aggtttccgg tacagctgca acagccaagg
     4621 aaatcgaagt catgaacatc tcaaggagtg gttctccttg cagttcacca atcacaaata
     4681 ctaaagccgc agccgctaaa gcgatcatac tgattcgttt gcctagctga ttcaatcgga
     4741 cctgtaaagg tgttttttgc atagtatttt cgttcaaaag atccgcgatc ttacccattt
     4801 ctgtagccat gccaatggca gtgacgatcg ctttcccacg acctgcagca accgtacatc
     4861 ctttaaatac catattaagt tggtcaccta atgactcatc ttcttcagca ataaatgctg
     4921 catctttttc aactgcttca ctttctccag tcaacgccga ttcttctaca cgtaattgtg
     4981 atgcttcgat caaacgggca tcggcagaaa tagcatcacc ggactctaaa acaagaatat
     5041 cacccactac cagttgctct gactcaacag tggtcacttt gccgtctcgt atgacagttg
     5101 ccgtttgttt attcatatct tgcaaggatt ccagtgcttt ttctgcgttt ccttcttgga
     5161 caatcgccaa taccgagttg atcacaacga tagcgatgat cagcagtcct tcaaataaat
     5221 ccccatgttc tgtagcaaat gcggtataaa aggaaatcac cgctgctaca agaaggatga
     5281 tcgttgtaaa atcggacaag cttcgaaaga attttttgat catcgattct ttcggcgcct
     5341 catcgaattt attcaatccc tgcttttttc gccgttcatc cacttcttgt gaagacaacc
     5401 cagtatctgg atctgcttct gttaccttct ttacttcttc tattgtctgc ttgtattctt
     5461 ccatcctatt cctcctttaa aatcttcgta caaaaaaagg tacttatttt actataacat
     5521 ccttttttta tttacttcaa ataaagggct tatcgattat ttgttaagaa agcgtaaaat
     5581 ttaaagtgct tatatttgca aacgctacca aaggatgcta ttatatagat gtaaggaagg
     5641 aggataccat caaatagaca gaagaatttc tcgatagaac agtgtaagca aacaaagttg
     5701 ttagaagtac aggtttcacc cagacatgcg tgtagagtac aaacctataa atagtgaatg
     5761 cgtatggatg tttggatcac gaagtatccg aaacaagaag taatgcgata cgtaacaatt
     5821 gaataacgta aacgtctgaa aattgaatag gaacaagaga tgtttcaaat gttgaagatg
     5881 taacgaaaac aaaatttttg tttaacatga agaaattcaa caccgtgctt tgcatgtatg
     5941 atggaaagtt acaatcattt ctgtttattt gaatccttcc gaggtacaag aaagccgagc
     6001 ttatcgagag ctcggctttt tctttgtcat aaaatctttg taaagctttt ttgttattca
     6061 aaactagcgg cttttcataa ccttatttca cgaacatcgc atctccaaaa ctaaagaaac
     6121 gatagcgctc ttcaatcgca tgctggtaag cagataaagt caattctctt cctgcaaagg
     6181 cactaactaa catgactaaa gtcgattttg gcaaatggaa attagttgag aaagcttcta
     6241 ccactttgaa ttcatagcca ggcgtaatga aaatatcagt ccatccgctg tctgctttga
     6301 tttctccatt aaatttcgtt ccaatcgttt ctaatgttcg aattgaagtt gttccgacag
     6361 ccacgatttt cccgccattt ttacgtactt ggttcaatcg ctcagccgct tcttccgtca
     6421 aacggtagaa ttcactatgc atatggtgtt cttcaatttt atctacgctg acaggtcgga
     6481 atgtacctaa ccctacatgt agtgtcagat agactaattc aacacccttt gcttcgatct
     6541 catcaagtaa ttctttcgta aaatgcaatc cagctgttgg cgctgcagca gaaccatttt
     6601 ctttcgcata gacagtttga tagcgatctg ggtcttccag acgttctttg atatatggag
     6661 gcaatggcat ttctcccaat gattcaagaa tctctagaaa aattccttca tactcaaagt
     6721 caatgatccg tccgccgtgt tccaattctt ctttcactac tgcttgcaat ctgccatcac
     6781 caaaagagat tcttgtccca actttggcac gtttcgcagg tttgatcaat gtctcccacg
     6841 tatctccttg tgtattcgtt agaagaagga cttctaaatg cgcaccagta tctggttttt
     6901 ctccatataa acgagcaggc agaactcgtg tattattcat aactagcgca tcaccaggct
     6961 gtaactcttc aatgatttca tggaaatgtt tatcttccat ctctccagtt ttccgatcca
     7021 aaacaagtaa acgagaatga tcgcggtctt ttaatggtgt ttgtgcgatt aattcttccg
     7081 gcaagtcaaa atcaaaatct tctgttgtta acatatcgtc actcactttc tatcatgtgc
     7141 ttcatcattc tatcatgttt ttcgttctgt tggcaaaatc agcacttttt cacttctatt
     7201 ttttgttttc ctaaataaaa aaactaccct atttgtattt tttcattgtt ttaggcatac
     7261 tagtaataga tagttcttca ttgaaaatag aaaaagaggt ggcaccatga ttgaatttca
     7321 aaatgtgtca aaaatttata aaggtggaaa agtcgctgta gaggacgtca atctttcatt
     7381 tgaaaagggt gagttcattt gttttatcgg tacgagcgga agcggaaaaa cgacgacgat
     7441 gcggatgatc aaccgaatga ctgatccaac acaagggaaa attttgattg atggaaaatc
     7501 catccaagaa attgatccag tggaattgcg acgtcagatc ggctatgtta tccaaagtat
     7561 tggattgatg cctcacatga cgatccgtga aaatatcact cttgtccaaa aattgttgaa
     7621 agtcagtcaa gaagaacgga ataaaacagc tgaaaaaatg attgatcttg tggaattgcc
     7681 ccgtgagatg ttagatcgct acccacatga attatctggt ggtcagcagc agcgtattgg
     7741 agtcgttcgt gcccttgcag cgaatcaaga tatcatcttg atggacgagc cattcggtgc
     7801 gcttgatcca atcactcgtg attccttgca agaccttgtc aaagatttac aagagcgttt
     7861 gggaaaaacg atcgtatttg taacccatga catggatgaa gcgattaaac tagcaagccg
     7921 tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa atattttacg
     7981 tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac tacaagccaa
     8041 gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga ttacaccgga
     8101 aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata cgttgttagt
     8161 taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga ataaaaaacg
     8221 aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg taaaagaatc
     8281 cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat acgtgccggt
     8341 cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag tggatatcgt
     8401 ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt cagctactcc
     8461 tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg gttcagaaat
     8521 tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca ttggtatcat
     8581 cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca cgattgtgat
     8641 tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat tgatgatccc
     8701 gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt cattattgcc
     8761 gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc gggatgtggc
     8821 aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc ccattgctat
     8881 gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat gggctacact
     8941 tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac tgaataatta
     9001 ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac tattggctga
     9061 ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag aggagaatga
     9121 agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat acttgcgagt
     9181 tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat cacaggtgga
     9241 atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga acattataca
     9301 gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca tcaagcgatg
     9361 atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt aacgactaca
     9421 ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa agatgaattt
     9481 gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac gtatgtcttt
     9541 cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga tctgaaaaat
     9601 gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa aggagacggt
     9661 tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc tatgcagatt
     9721 ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg ctactctacg
     9781 gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca attcttccca
     9841 ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc tcatttaaaa
     9901 gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga attgaactac
     9961 gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt acaagaaaac
    10021 cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa caatttttct
    10081 attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac ttcctgattt
    10141 ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc atgatttcta
    10201 ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag acgatccctt
    10261 ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat gtcgtgattg
    10321 ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc gggatgatcc
    10381 aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct agacaacgac
    10441 tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata agaaacgcgt
    10501 tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt ggtcttggag
    10561 atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta gcaggtgcat
    10621 tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg gaaagacggc
    10681 tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag cttctgcgac
    10741 catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata atccactctg
    10801 attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca ttccgtatac
    10861 tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt tttttcttat
    10921 ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc ataccacatg
    10981 atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac atcctttggc
    11041 gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt cagctgcatc
    11101 atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa tcgggaactt
    11161 gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc catgggaggt
    11221 ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca actagacttg
    11281 gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc gatatattgc
    11341 caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt gccgttgata
    11401 ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt gacattcatg
    11461 ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt attgtatttc
    11521 gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca ataagacgac
    11581 tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg ataaacgaca
    11641 tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac tgccaacact
    11701 aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc ttgttttaca
    11761 taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg tggagatata
    11821 ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga acaaaaaaag
    11881 agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc ttctcacttc
    11941 actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca tcaactttgt
    12001 aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt gtaggtctca
    12061 gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga atcacttctt
    12121 caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc gcagagtttt
    12181 tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat cgatattcgt
    12241 aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg tttgctcaag
    12301 aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag cattgtttcc
    12361 actacgatca ctaataatct cgatttcagt atgattttca tttacctctt tcaataactg
    12421 ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg tctctcctcc
    12481 tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac aataaagcac
    12541 ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt tttagggagg
    12601 atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa cgtaaagtaa
    12661 ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag tctatgcaac
    12721 aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct tgaataacct
    12781 gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc gtcaagtctt
    12841 ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca ctatcgattt
    12901 gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac tgagttatgg
    12961 taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca catggcactt
    13021 aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat ttggcaaagg
    13081 attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa taggaagaat
    13141 aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc tttagctgag
    13201 aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt ttttgatttt
    13261 aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata ttttcacgcc
    13321 aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc tgccagcgtc
    13381 tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc gctgcatttt
    13441 tacttacgat atctacataa cggataattg gattttcaat cgagcgataa gaagagtgga
    13501 cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt tctttttctt
    13561 tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg atcgaacgtg
    13621 cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca agaacggtat
    13681 tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct gctacttttt
    13741 tataatgatc atggatctta taaaaaatga acataacgat tggcataatg atgaagaaag
    13801 gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat gagatgaacg
    13861 cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt ttccatttga
    13921 cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag tataacggaa
    13981 tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct agcgctagtg
    14041 tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg tgaggcatga
    14101 atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg gtgttagcag
    14161 caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata ccatgtccaa
    14221 agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg atcccatacc
    14281 aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc atcatcgtca
    14341 atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca ttactaatcg
    14401 cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc aaagcgatag
    14461 aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca gcacctgtca
    14521 cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat gtgtacaccg
    14581 gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc atcagtagca
    14641 aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc gctgatgtga
    14701 tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg atcaataaag
    14761 agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg acatatgctc
    14821 ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat gaaatcaata
    14881 aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca gacaaagcga
    14941 ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca tcagacgaca
    15001 ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca gattttaaag
    15061 gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta cacacaattt
    15121 tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta cgctcactga
    15181 ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa tatcaagcgc
    15241 tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt tttctttgaa
    15301 ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt tttgatatct
    15361 taacttttgg aagagagatg taaggctgct ttctacccat cattcttact actcgaagca
    15421 tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc tgcggtaata
    15481 agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt attacttgat
    15541 gcagagcagc ttttttacaa cct
//
LOCUS       DACEOE010000057        15419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEOE010000057 DACEOE010000000
VERSION     DACEOE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.059"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            124..1875
                     /locus_tag="IW774_001435"
     CDS             124..1875
                     /locus_tag="IW774_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1153786.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            1875..3659
                     /locus_tag="IW774_001436"
     CDS             1875..3659
                     /locus_tag="IW774_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153787.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            3985..4836
                     /locus_tag="IW774_001437"
     CDS             3985..4836
                     /locus_tag="IW774_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1153788.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVVQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            4933..6813
                     /locus_tag="IW774_001438"
     CDS             4933..6813
                     /locus_tag="IW774_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1153789.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEVEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAISGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(6853..7755)
                     /locus_tag="IW774_001439"
     CDS             complement(6853..7755)
                     /locus_tag="IW774_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1153790.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENKVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            7986..9389
                     /locus_tag="IW774_001440"
     CDS             7986..9389
                     /locus_tag="IW774_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1153791.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALNDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            9389..10822
                     /gene="ascB"
                     /locus_tag="IW774_001441"
     CDS             9389..10822
                     /gene="ascB"
                     /locus_tag="IW774_001441"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1153792.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            10967..11893
                     /gene="trxB"
                     /locus_tag="IW774_001442"
     CDS             10967..11893
                     /gene="trxB"
                     /locus_tag="IW774_001442"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1153793.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            12107..13834
                     /locus_tag="IW774_001443"
     CDS             12107..13834
                     /locus_tag="IW774_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1153794.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            13906..14199
                     /locus_tag="IW774_001444"
     CDS             13906..14199
                     /locus_tag="IW774_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1153795.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(14291..15151)
                     /locus_tag="IW774_001445"
     CDS             complement(14291..15151)
                     /locus_tag="IW774_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1153796.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            15243..>15419
                     /locus_tag="IW774_001446"
     CDS             15243..>15419
                     /locus_tag="IW774_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1153797.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDT"
BASE COUNT         4921 a         2644 c         3450 g         4404 t
ORIGIN      
        1 ttttacgggc tgattttttc agtccgtttt ttatttcgta atttgttaga ctattcagat
       61 tattcagcaa aaagacaagc acagacaatc ctaggctctt gataaagaca aaggaggagt
      121 aaaatgtcga tttttagaaa gttaggctgg ttttttcgtc aggaaaagaa acattattta
      181 attggggttt tctcgctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt
      241 gggatcatca ttgatgagat tgccgataaa aatttatcag taaagatcat tttcgggtgg
      301 atcttcctat tgattatcgc tgctttttta caatatcttt ttcgttatat ctggcggacg
      361 aatatttggg gaagtgcagc tcggttggag aaagaacttc ggcagcagtt attcaaacat
      421 tttacagaaa tggacaatct cttttaccaa aaatatcgga ctggggattt gatggctcat
      481 gcaacgaatg atttgaatgc gatccagaat gtagcaggag ctggtatctt gacctttgca
      541 gattcagtga ttacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc
      601 ttaacactga ttgcgctgat tcctctgcct ttgctggccg tcacttcacg agtgctgggg
      661 tcgaaactcc atgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag
      721 acacaagaaa gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat
      781 ctagcggatt ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt
      841 ttagactcct tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca
      901 attattttag gtggaaagta tgtcttggaa ggcacgatct cgatcgggca gatggtctca
      961 tttattagtt atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac
     1021 gtattggaaa gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaaaacg
     1081 catatcgtgg aacaactggg tgcagtgcag accccggcaa aaggcgagct gaatgtagct
     1141 atttcctctt ttaattatcc gaaagaagaa tcaccagcat tagaacaaat caagtttgca
     1201 atccaagaag gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacgatc
     1261 atcaaattac tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt
     1321 tcgatcaaag aatattcatt gaatgcttta ttaggatcaa tcggctatgt gcctcaggat
     1381 cacttcttgt tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca
     1441 gaagaagaag tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaatta
     1501 ccaaaaggtt atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa
     1561 caaaggattt cgattgctcg agcattgatc aaaaatcctg aattattaat attagatgat
     1621 gcactttctg cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata
     1681 agaaaagaga aaacaactat catttcagct caccgtttaa gtagcgtgat gcatgcaaaa
     1741 gaaatcattg ttgtcgatca agggaaaatc attgaacgag gaacccatca tgagctgctt
     1801 gcactaggcg gctggtatga acaaatgtgg gagaaacagc aattagaagc gaagatagaa
     1861 ggaggcgagg cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta
     1921 aagaacagaa aaagatcatc aaacgactga tgaagttcgc caaacctttt agaagaacgt
     1981 tcatcgttgc tatcttattt gcgtttgctc tgtctgtgat caatgtccta ttgccaagga
     2041 tcatccaaac ttttatggat gatcatctgg ctaaacagtc ggctacgaca caagttattt
     2101 tgttttttgc tggtatgtac ttatttggag tcatagtaaa aagtatcatt tggtttttcc
     2161 aatggtattt gtattccatg gcttcattga agacctatca gtatattagg gtaaaattat
     2221 ttgaaaagct gcatacacta ggtatgcgtt attttgatca gacaccggca ggatcaaccg
     2281 tttctcgagt gacgaatgac acagaaacgc tatttgaatt ttggtacgtc tttttgatgg
     2341 tattgactgg gatctttgca gtcgtttctt cattcattgc catgtttcaa atcaatggag
     2401 aaattgcctt gtggactttg gtgttcttgc caattttagg aatagtcatc tggtattacc
     2461 agaaatttag ttctcgtatt taccggaata tgcgggaaaa attaagtcag ctgaatacga
     2521 aattgaatga atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt
     2581 tagctaaaga atttgaacaa gtgaacaatg agtatttaga cacgcgttat gcgatgatca
     2641 aaacaaattc gattctgtta agtccgatca tcaattttct atatgcattg gcgattgcgt
     2701 tggtgttgac tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct
     2761 atgcatttac gacctatgtc cagggatttt ttaatccaat gtctcaaatg atggactttt
     2821 tgagcatctt cacagatggg atggttgctg gtagtcgtat cttgaagatt atggatacag
     2881 aagaatatgc accacaacaa aaccctggag cagatcaaat gattacttca ggtaagattg
     2941 agtttcgcaa tgtaagcttt tcttatgatg gagagcacaa tgtattaaac aatatctctt
     3001 ttgttgctaa tccgggagag accgttgcat tagtgggaca caccggaagt gggaaaagct
     3061 cgatcatcaa tgtgttgatg cggttttacg aattttatga aggtcagata ttgattgatg
     3121 atcatgacat tcgagaatat ccaatggaag agttacgtaa gaaaatgggg ctggtcttgc
     3181 aagatgcttt cttgttttat ggggatatcg caggaaatat tcggatgatg aatccagcta
     3241 tcacagacga acagatacgc catgcggctg agtttgttca agcaaacaaa ttcatcgatg
     3301 aattaccaaa aggatatcac tcaaaagtga tcgaacatgg tgccagctat tctagtggac
     3361 aacgacagct gatcactttt gcgcgaacaa tcgtgactga tcctaaaatt ctagtattgg
     3421 atgaagcaac agcaaatatc gatactgaaa cagaaggatt gatccaagaa ggattggcga
     3481 aaatgcgaca aggacggaca acgattgcga ttgcccatcg gctgtcaacg attcgagatg
     3541 cagaattgat tttagtttta gacaaaggtc atatagtaga acgcgggaat catgaaacac
     3601 ttctagcgca aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtatataaa
     3661 agatgttgtg acagtagcat tttgacctaa gcactagagt gaaaataaaa catagcttcc
     3721 catatttttc atctgtgaga gttagtgtcg tagttaccag atttaaaaaa gacgacgtca
     3781 gctagtcaga agtgcctaat agcccctaag aaagcgttga caaaatcgtt tgctttcgat
     3841 aatataaaga aaacgataca acttggatag tgattgcctg atgcaagcta aacctaatga
     3901 gtctcccatg gtttcatgga cggctttttg ggtttttctt tttgttttgg atacagccct
     3961 atatatcatg gaaaggagca aaacatgctg attgagaaag tactgaataa caacgtcgta
     4021 ataacaaggg atgaaagaga tcgggaaatg attgtgatgg gaaaaggatt ggcattcaaa
     4081 aagaaggtgg gtgacccgat tcttgattca tgcatcgaca aaaaatttca gttgacaaat
     4141 gacggacttt cacaacagtt ccaagaattg gcacaagcga ttcctcttga gtacttagaa
     4201 atttcttgtg aaatcattga atatacaaaa gaggctctgc aagtccattt aaatgattct
     4261 gtgtatattt cattgacgga tcacttgtat acagctatcg aacgtcaaaa aatggggatc
     4321 tttgtgccaa atgtattgct gtatgatatc aaacaactgt ttcctaaaga gtatgccatt
     4381 gggaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagacgaa
     4441 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca gcaagacaat
     4501 acctacctgt ttaccacaac aatacaggat attttaaata tcgtccgcta ttactataag
     4561 attacatttg atgaagattc agtccacttt tttcgattcg tcacacacct gcgttttttt
     4621 ctgacacgtg ttctagatgg agcgaaatca gaagatggag aagtttctga ggaattgttc
     4681 caagttgttc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
     4741 ttaaccgggg ctttcgacta ccagatgtcc acggatgaaa aaatgtattt aacgatccat
     4801 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaggagga caaattaaca
     4861 agaacgtatg aaaataaggg tagtgacatt cagttggcta aaccttccac taattaattg
     4921 gagggaatag aaatgggaaa ataccaggaa ttagcagaaa acatcgtaaa gaatatcgga
     4981 ggaaaagaaa atatccgtag cttgacacac tgtgtgacgc gacttcgttt caaactaaag
     5041 gaggaaaata aagcaaagga tgatgttttg aaaaagatgg aagggatagt caccatcatg
     5101 aaaagcggcg ggcaatatca agttgtaatc gggaatcatg tgccagatgt ctatgcagaa
     5161 gtactgacag tagccgggat ttccgatgaa gtagaagaag aggtttcttc aagtagtaat
     5221 ctgttcaatc gattaatcga catattaagt ggctgtttcc agcctttttt aggtgcttta
     5281 gctgcagcgg gaatggtcaa aggattaaat gctttgttct tgtttctcaa attttatact
     5341 gaaggatcgg gtacgtacat catgctgaat gggatagggg atgcaatttt ttatttcatg
     5401 ccagtgatct tagggtacac agcagcgaaa aaatttcatc tgaatccgat ggtggggctc
     5461 gtgattggtg cagcactttg ttatccgact gtacaaggag cagcactaca aagtggtttc
     5521 cctgatggca gtcctgcgcc ttatagcttg ctgggtcttc cggcttatga aacatttttg
     5581 ggtatcccat gggtgggcag cagttatacc agtagcgtcg tacctgttat cttcattatc
     5641 gcatttgccg gacaagtcca aaaaattgcc aagaaatgga ttcctgcagt cgtccagacc
     5701 tttttggttc ctttttctgt gttattaatc gcacttcctg ctggcttttt gattatcggt
     5761 ccgattgtca gtatactgac agatctatta agcacgggat tccaagcatt gatggacttt
     5821 tctccagctg tgtatggtgc agttctaggg tttctttggc aagtactggt gattttcgga
     5881 cttcattgga gtattatccc tcttgcgatt atgcagctag cgcaaaatgg gtggagtcag
     5941 atgttagtag gcgtttatgg cgcgagtttt gcacagacag cagtggtatt ggcgatgtat
     6001 ttcaagttca aggatcaaaa aatgaaggcg ctttgcatgc cagctgtgat ttcaggaatc
     6061 ttcggcgtaa cagaaccggc tatttatgga atcaccttac cgaagaagaa gccatttatt
     6121 tactcgatga ttggtggtgc catttcagga atctatttga tggcatctgg aacaatcagc
     6181 tacacaatgg gcggattagg gatttttggg gtggtaaact acatcacgga aacagacgcc
     6241 agtggtatgt ttcaagcgtt tatcggcatt gctattgctt cggtaatcgg gttttcgctg
     6301 acttttttct tttggaagga tgaagcaggt gaaacagatg ctatacagaa aaaatcaggt
     6361 aaagaaattg taaaaacacc gatacaagga caaatcgctc cactaaatgc agcgaaggat
     6421 gcagcttttg cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa
     6481 gtacgtgccc cttttgacgg aacagtcatg accctgttcc cgacgaaaca tgcaattgga
     6541 atggtttctg aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg
     6601 gaagggaaat attttgaaag cttagtgcag caaggagaaa gagtgaaaca aggcgatctg
     6661 ttgctacgct tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg
     6721 atcgtcacca attacacgga ttatttggat attttggaaa accaagggga gacggtcggg
     6781 ccagacgatg cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggaataggct
     6841 taactattct ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc
     6901 tgagctgata gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg
     6961 ttgataattt tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt
     7021 attgatcgta aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc
     7081 ggctgtactc attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac
     7141 atgattttgt ttcaatattt ctgcggtacg aagaatcact ttattttcac ccgtataaga
     7201 aatcaggatc ccgcacatat cgctcgtgca attgtacgct tcatagaatg attcaccaaa
     7261 agtggtgaca attgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga
     7321 aatcaaagta ttcgagttac tggcgaagat cacgattttt tttgcctgga gcagtaaata
     7381 cttggctttt tgcagttggt catgatgaat gagagaaagt gtatcttgaa tcgtcatctg
     7441 ttctaaagaa gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag
     7501 attcgcatct acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt
     7561 acgaaaactg acccagccat caaaacccat tttttttgct acacggatga gtgtagacgg
     7621 gtggacatag catcgctcag cgatctcttg gatggtcatt tcgccaagtg ctggagaatc
     7681 gcttaagaga aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt
     7741 ttcacgtaat aacatgtagt taccttcttc ttattttttt acgtaaatta taactttttt
     7801 gaaaatgttt tgtaaacgga tttatactat ataacttaaa atagactttt tttgacaaga
     7861 tatttacaaa aatccactag aaaacagtaa cttttgtaaa tgttactgga aagaaacgga
     7921 tttctattgt ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg
     7981 agaacatggc aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccctg
     8041 agttgatgga aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg
     8101 tagatgtaga agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt
     8161 gggatgcttc tccgtatgaa gtcaaaatct atgcaactct ggatcgggaa gaagcattga
     8221 atgatgcgga ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag
     8281 acgagcggat ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga
     8341 tattcaaagc ttttcgtacc gttccagtgg tcttggcaat cgttgaagac atgaagcgtt
     8401 tatgtccaaa tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag
     8461 ttatcagata tggcaaatgg gataaagtga ttggattatg caatgtgcca gtaatggcga
     8521 tgatgacaga accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg
     8581 gtctaaatca tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag
     8641 aattgattga tcgtttatac gatgaagaca atggattgcc gaaaaatatc tttgatatcc
     8701 cgtttttcaa agaacaatta aaacaaatga atatgatccc ttgtggctac caccgctatt
     8761 actaccgtga agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa
     8821 gagcgcagca agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat
     8881 tagaccataa acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct
     8941 gtgaaacgat tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga
     9001 atgatggagc tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg
     9061 gcgcacaagg agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt
     9121 tgcaagtaat gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg
     9181 ggacagcttt acaggcgttt acgatcaatc cgcttatacg atcaggaaag acagcaaaac
     9241 ggatcatgga tgagttgttt attgcacacc agcagtattt accgcaattc aaggaaacaa
     9301 tcagccgatt aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag
     9361 gtaatacaca tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg
     9421 tgctacagca gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa
     9481 cgtagatgtt gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat
     9541 gtttgaattt gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg
     9601 ttataaagaa gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat
     9661 cgcctggagt cgtatttttc caatggggga tgaaaaagag ccgaatgaag ctggcttgaa
     9721 attttatgag gatgtcttta aagaatgcca aaagcacgga atcgagccac ttgtgacgat
     9781 tacgcatttt gattgtccaa tgcatttgat caaagaatac ggtgcgtggc gttccagaga
     9841 actcgttggt ttttatgaaa atctgtgtcg agtcatcttc aatcgatata aaggacttgt
     9901 caaatactgg ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggagc
     9961 aggactgtac tttgaagaag gagaaaacga agaacaagtc aaatatcaag cggcacatca
    10021 tgagttggtt gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa
    10081 agtaggctgt atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt
    10141 ttgggcttct agaaaatcag atcgagaaaa ttatttcttc attgatgttc agtcacgagg
    10201 agaataccca gcttatgcat tgaaagaaat ggaacgaaat ggcattcaga tcgaaatgga
    10261 accaggggat gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta
    10321 ttcttcccga gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt
    10381 tgcttctgtc aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt
    10441 aggcttgaga ataacgatga atgatattta tgatcgttat caaaaaccat tatttattgt
    10501 agaaaacggc ttaggtgcag tcgatacgcc agatgagaat ggttatgtgg aagatgatta
    10561 ccgaatcgat tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg
    10621 agtcgatcta ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg
    10681 tgagatgaag aaaagatacg gattcattta tgtcgatcgt gataaccaag gaaatggcac
    10741 attagcccgt tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg
    10801 agaaaatctt tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt
    10861 tttcgagtat aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat
    10921 tggctgtaaa gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat
    10981 cgttattggc gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt
    11041 atccgtattg atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat
    11101 cgaaaattat cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga
    11161 aagtgtctca agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca
    11221 cggcagccat aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat
    11281 cgcaacaggc tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg
    11341 aggcgtttct tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt
    11401 catcgggggt ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga
    11461 agtagtgatc gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc
    11521 atttgcaaat gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa
    11581 cgaaatggta gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt
    11641 agcagcagac ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga
    11701 agcaggtttg acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat
    11761 gccaggcgtt tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc
    11821 tgttggtgac ggcggtattg ctggacaaca agtgtataaa tacttggaat ctttagaaga
    11881 aacaactgca taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt
    11941 ttttcgcgtg aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa
    12001 agaatccttt cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa
    12061 tgctatactt attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca
    12121 agtgtatgaa caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa
    12181 tgatctgggt gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg
    12241 tactgcaggc atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt
    12301 aagacaagca acagaaggac tagcacgttt catggataca caaggtgaag aagcaaaacg
    12361 ccgaggtgtc gctattgctt atgactcacg ccatcagtca ccagaatttg caatggaagc
    12421 agccaaaaca ctggcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac
    12481 accagagctt tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaactgc
    12541 atctcacaac ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat
    12601 gcctcctgca gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa
    12661 agtcgaagta ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga
    12721 agaagtggac gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt
    12781 agaaacaatg gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat
    12841 gttgggcgaa cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca
    12901 agctgtggca gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc
    12961 ttttgaatat gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga
    13021 tcctgatgca gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt
    13081 gactgggaac caaatcgggg cattgtttgc tcaatatatt ttggaagcac atcaatcaag
    13141 aggcacttta ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac
    13201 agcaattgcg gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat
    13261 tgctgaaaaa atccaacaat tcgaagaaga ccattcacat acttttatgt ttggattcga
    13321 agaaagttat ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt
    13381 gcttttatta gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg
    13441 cttgcaaacg atttttgaaa cctatggcta ttttgcagaa aaaacgattt ctgtgacaat
    13501 gagcggacaa gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc
    13561 accgacagaa tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag
    13621 aacagaccgt gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaaata
    13681 tacattggaa gaaaatggct ggattgctat ccgcccttca ggaactgagc cgaaaatcaa
    13741 gttttatatt ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta
    13801 tgaagcgatt atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg
    13861 gtaaacttta gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa
    13921 aaatcaaaag tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt
    13981 aaaattctgc tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg
    14041 gaaatggagc agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc
    14101 aagtctcatc gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat
    14161 attttgaacc agacactcaa acatgtggaa catcaataac gataggtgca aatagataaa
    14221 cactggagtg tgacaacgaa atttcttcgt tgtcacactc cagtgttttt taagaaaaag
    14281 acaaaaatga ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta
    14341 ggtctaaagc attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga
    14401 tcactacaca tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt
    14461 tttcgatatc ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac
    14521 ggatattatt taaaatatct gggttattcg ttagtcccac gattttttta ggatcgatct
    14581 cccaaagctg tttgggaata tgtgcttgtg ggatcaaagg taaattggca acttttaaat
    14641 ttttgttagc taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa
    14701 cgacatctgc ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt
    14761 ccattgcttt gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc
    14821 gaattggctt gataccggtt aaacgttcaa tttctgatat cggtggcgtc atcacatcaa
    14881 aactaaataa ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga
    14941 ttaatgtatg caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgctttaatg
    15001 ttttttcatc tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag
    15061 ccatagtagc ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa
    15121 aagtaacgat ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat
    15181 gtttattata ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag
    15241 tcatgaaaaa acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta
    15301 aatatactaa aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat
    15361 ctgctaaaga agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacg
//
LOCUS       DACEOE010000058        14833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEOE010000058 DACEOE010000000
VERSION     DACEOE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.060"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            190..1086
                     /locus_tag="IW774_001447"
     CDS             190..1086
                     /locus_tag="IW774_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1153798.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1236..2177)
                     /locus_tag="IW774_001448"
     CDS             complement(1236..2177)
                     /locus_tag="IW774_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1153799.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2303..3424
                     /locus_tag="IW774_001449"
     CDS             2303..3424
                     /locus_tag="IW774_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1153800.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3682..5184
                     /locus_tag="IW774_001450"
     CDS             3682..5184
                     /locus_tag="IW774_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1153801.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5251..5778
                     /locus_tag="IW774_001451"
     CDS             5251..5778
                     /locus_tag="IW774_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1153802.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5989..7050
                     /locus_tag="IW774_001452"
     CDS             5989..7050
                     /locus_tag="IW774_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1153803.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7053..7949
                     /gene="murQ"
                     /locus_tag="IW774_001453"
     CDS             7053..7949
                     /gene="murQ"
                     /locus_tag="IW774_001453"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1153804.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7963..9945
                     /locus_tag="IW774_001454"
     CDS             7963..9945
                     /locus_tag="IW774_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1153805.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10010..10456)
                     /gene="nrdI"
                     /locus_tag="IW774_001455"
     CDS             complement(10010..10456)
                     /gene="nrdI"
                     /locus_tag="IW774_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1153806.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10667..12091
                     /locus_tag="IW774_001456"
     CDS             10667..12091
                     /locus_tag="IW774_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1153807.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12272..13045)
                     /locus_tag="IW774_001457"
     CDS             complement(12272..13045)
                     /locus_tag="IW774_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1153808.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13042..13947)
                     /locus_tag="IW774_001458"
     CDS             complement(13042..13947)
                     /locus_tag="IW774_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1153809.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEEYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14101..14421)
                     /locus_tag="IW774_001459"
     CDS             complement(14101..14421)
                     /locus_tag="IW774_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1153810.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4764 a         2632 c         3120 g         4317 t
ORIGIN      
        1 acaaattttc agacaattac aactattcag aaaactttta ggcgaatttt aatagaagaa
       61 tgatttattt catcatattt tcatacaaat gttaaatttt catacaaata taggaaacgt
      121 tttactggct ttttactatt taaacaggct atcataggaa aaagtgacag agaaagtgag
      181 gcggaaaaga tgaacaaaaa tgacgtactg ctagttgcaa aaaataaatc actttatttt
      241 ttgccttcca tccgctcttt agatgtttgg tccggaaaag atttactaca gtcggaattc
      301 attaaatcag ctagacaaca gttggcaaat tatcagctga tcgttttttt agattatgga
      361 tttgatatgg agatggctcg acgcgtgcgc ccatttacca atgcaaaaat tgtgttattt
      421 ttttggaatc acttcaaagc agaacacgag gcgatgctga agcaggcaca agatgaaccg
      481 gcaatcgatg agatttatca ttttgatttt ttggaagcaa aagaacttgg gctgaaacat
      541 aatagttcat tttattcgaa acatatgaat ttttcttcta gcgaaccaaa gacggatcta
      601 ttttttggtg caacgaataa tgggagaaaa gaacgagcag aaagctataa gcaggaattt
      661 ataaaaagag atgttacgac caactatttc attcttccac atcgagggaa tgaacaacct
      721 ggatatctta cttatgaaga gtacctgaaa aaaacagggg aatcgcgcgg gattctggaa
      781 ctgttgcggg agggtcagca aggagtgact ttgcggacat ttgaatccat gtattttcaa
      841 aagaaactag tgacggataa tcaagcgatt gctcattatc atttttatcg tcctgaaaat
      901 atttttttgc ttcgagaacg tgatttagat gaattaccgg aatttctgca tacaccattt
      961 aagccaatcc tatcagagct attggaattt tatgatgcag aaaattgggc aaaacgattt
     1021 atcactaacg attccactat ttatgagcaa tacgaatatg acctctcctc tgttgaaaac
     1081 aaataaaaaa cctacatttt gaagagatta tgtactgaaa caaacaagca caattcttat
     1141 ggcatcatga gagttgtgtt ttctttttga tcttttattg ggcttaaaaa aagagaacca
     1201 gctgctgatt cagctgattc tcatcagaga gccttttatt ctgcgccaag gattccttct
     1261 tttgcatgga ctaattgggt caaaaaggca caatatggtg tagcaatatc atatttttgt
     1321 ccttttcgcc aaatggcacc attgatgtag tcgatttcag tcaaacgatg gtttttgatc
     1381 aggtcttggt acatagatgg gtaatgcaac ccgatgttgt caggatcata agtagaagca
     1441 atatgttggt acacttcttc ttgatctagt atgattcctt cttttgctgc gatagaagca
     1501 aattcagata cgattgttcg aaccatgctc tctgccgctt tttgcgcgcc tagttcagca
     1561 atattacaat ccaaaatcgt gcataaacca ttcaatgttc catttacaca agcttttcgc
     1621 caaatggaat aacgaacgtt atggctatag atcggattta aatttgcttc ttgaaagact
     1681 tctacaactt tttttgtaaa tgcttcgcct tctggttcca agttctctag ttcgatttct
     1741 ccatcaccta acaatgtgac ttttccggga ccagctagac cagctgtcca catcgtgatc
     1801 ccaaataaga tatttttctt tggcacatat ttttccagta catcttcatg acctaatcca
     1861 tttagtaaac acaaaacata agtattgtca gtaataatgg attggatcga acaaaacata
     1921 tcatctaatt gattggcttt tgttaatgct acgatcaagt ccacgtgttc gttgctttcg
     1981 atgatttctt ctggtgaata gattggcagt tttgcgacca cttctttacc gttaaagtca
     2041 gcaatcaacc catttgtacg aatggcttca atgtgagcag gccaacgatc gatcaagagg
     2101 acctcatttc cgctttgatg tagcatcaat ccgattcggc tgcccattgc tccggcccca
     2161 gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt ttagtatata
     2221 gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag tgtcttactt
     2281 aagaagaaag tgagggatac agatggatat tttggcggca attgattcat ttaaaggaag
     2341 tgcttcttca tttgaactaa atcaggcagt tctaaatggc tgggaagatc gtaaaggaag
     2401 aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa tctattacgc
     2461 gctcagggga acatggcgta ccattgatgg gatggatctc ttattccgtc agcgaaaaat
     2521 cagctattta ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc
     2581 attggggtta gatctacttg ataaaccaac ggatcaaact atccgacttg cttccagtta
     2641 tgccttagga gaacttatta aagatgcact gcttcaagaa gtagaacaaa ttattgtcac
     2701 tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct tgggtgcttc
     2761 tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc attttgatcg
     2821 aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta ccaatccata
     2881 tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa tagatacgac
     2941 gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag atttctggga
     3001 ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg gagccctttt
     3061 tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca ctggattgga
     3121 aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg atcagcaaac
     3181 gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat
     3241 aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt ttctcccaat
     3301 tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac acaggcaaac
     3361 attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca tcctcccgaa
     3421 gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga caaagtgagg
     3481 gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg
     3541 gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga tgtatgggtc
     3601 tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct
     3661 ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac
     3721 gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa
     3781 ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac
     3841 aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag
     3901 acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata
     3961 tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa
     4021 ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat
     4081 gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa
     4141 caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg
     4201 ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt
     4261 tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg
     4321 gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa
     4381 gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat
     4441 actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa
     4501 cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta
     4561 aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga
     4621 cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat
     4681 tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca
     4741 tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag
     4801 ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg
     4861 gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag
     4921 cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg
     4981 aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac atgggatgaa
     5041 ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg
     5101 aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag
     5161 attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag
     5221 gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca
     5281 acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga
     5341 cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa
     5401 atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc
     5461 agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga
     5521 ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca
     5581 aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc
     5641 gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat
     5701 gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa
     5761 ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg
     5821 cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata taaactcaac
     5881 ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt ctttagatat
     5941 atactctagt catagaaatg aagacggata cagaaaaaga gggataatat gtttggcttt
     6001 tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg ccaaatggca
     6061 tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga tgtttcggca
     6121 tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact agaattgatg
     6181 gtcgatattt caggagaggc attggaacga gcgggttttt cattagaaga tctaacgcct
     6241 ttgaaagcaa ttggtgtaac aggtttaaga atggattatc atatttccaa tcagcagatt
     6301 gccgactggt cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat
     6361 atcgatgaat tgaaagaagc agaagctgat ttctcacaga ttgaagcttg gcataattat
     6421 tatccacgtc ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc
     6481 aaaattcaag gtctccttgt ccaaggtttt gtaccaggag atacagagtt gagaggccca
     6541 ctatatcaag gattgccaac tttggaagaa catcgaggga tccatcctct tgcaggcgct
     6601 ctagatctat tagcaagtaa cacggatatt gtttacatcg gagatgcagg tctgtcagaa
     6661 aacgttcagg agcaatttgc ttctttccaa aaagaacaga cagtcttgct ccatacggaa
     6721 cctgtagatg aagaatttta cgaatatata ttagggaagc atacgaatcg gcaagacgat
     6781 gcaagagatg tcatacgaag cgtagatgca cgttttcgag agattccgcc tatcccagca
     6841 agaaatacag ctacgcgtat gaaagggtct attactttag ataacgaaaa gtatttgcgc
     6901 tatatgggtg agatccaatt aaccaaatat gatttgccag cggatgaaaa agtgaatgta
     6961 gtagctaaag ttatcaaaga agagctacca ttgatcaacc aaatcaaagc tggtatgaac
     7021 tatcaattta taagaaaaga aggaagataa aaatgagcaa aatcaattta gagaacctaa
     7081 caacggaacg cagaaaccaa gaaacttttg gattagatga gatgagtatt tctcaagccg
     7141 ttcaactaat gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa
     7201 gtatcgaacc agtgattgct aacacgattg agtcctttaa aaaaggtgga cgtctgattt
     7261 atatgggagc cggaacaagt ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga
     7321 cattcggtgt agaaccggaa atggtcatag gtctaattgc tggtggagaa gaagcaatga
     7381 ctgtagctgt cgaaggggcc gaagattctt atgagctagg gcaagaagat ctaatcaagc
     7441 tgaatttgac aaaaaatgat atagttattg gtatcgcagc tagcggtcgt acaccatatg
     7501 tgatcggtgg attagattac gcaaaatcga tcggtgctgt cacaggaacg atttcttgca
     7561 ataaacaagc cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat gcaggtccag
     7621 aatttttgac aggctctact cgattgaagt caggaacagc acaaaaactt atcttgaata
     7681 tgatttctac tatcagtatg attggtatag gtaaagtatt caataacttg atggtcgatg
     7741 tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg caagcgacag
     7801 aagctgatta cgaaacagct gaaaagtact ttgaagaagc agatcaaaat gttaagctgg
     7861 ctatcgtcat gattttgaca aatagtgata aagaggaagc tcaagcaaaa ttagctgaag
     7921 caaacggatt tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa aaacgaactg
     7981 actttggaag aactggcaaa acaaatttat aaaggggcag gcggcatggg caatgtagaa
     8041 agcattgtcc actgcatgac tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat
     8101 gtctctgaat taaaagcgat tccaggagtg cttggtgtag tagaagatga acagcttcaa
     8161 gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc aaatggttga tttggcaggc
     8221 gtgaaattag gagaaaaact ttcttctgta agtacagcta cagcaaattc atcagcatct
     8281 ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa
     8341 aaaccatcta aaatcaaatc ggtattaaaa gacatttcta atatctttgt gccaatgatt
     8401 cctgcatttg tcggtactgg gatcgtggca gggattgccg cagttttagc aaacttagtc
     8461 acagccggaa cacttgatgc agcaacatgg caacaatata tcgatgtgat gaacatcttg
     8521 aaaaacggga tgtttagcta cctagttatc tatacaggta tcaatgctgc acaagttttt
     8581 ggtgcaacac caactcttgg tggagttatc ggggccgttg tgatgctgac agggatgaat
     8641 ccggaagcac cactgaaaaa cttatttact ggtgaagctc ttgcagctgg acaaggtggg
     8701 atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa actgcataaa
     8761 gtagtacctg atgcggtaga tatcatcgtt acaccaactc tttcgttgat tgccatcggt
     8821 ctgattgaaa ttttcttcat catgccttta gcaggtttca tttctgatgg catggtcggt
     8881 gggatcaact gggtacttgg cgttggtgga gcatttgccg gcttcgttct aggcacatta
     8941 ttcttgccga tggttatgtt cggactgcat caaattttga ctccaatcca tgtgcaaatg
     9001 attgatgaaa ctggtcgtac gctattgctt ccaattcttg cgatggctgg tgcaggacaa
     9061 gtgggtgcag cattggcatt atggattcgc tgcaaaaaag ataaagaatt aacagaaatg
     9121 atcaaaggag ctttaccagt cgggatccta ggaatcggcg aaccattgat ttacggagta
     9181 acattacctc taggacgccc atttattact gcttgtatcg gtggaggtat cggcggggca
     9241 gtgatcggaa tgatcggaaa tgtcggggct atcgctattg gaccttcagg agccgctttg
     9301 atcccattga tctctgacgg taaatggtta ggctacatcc ttggattatt ggcagcttat
     9361 gctggtggct tcgttgctac attcttcttc ggtattccta aagaccgttt agaaaaagaa
     9421 gaattagcga ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt acctcaaact
     9481 tcagctgaaa gtgtgactat cctctcatca gttgctgacg gaacagttgt ttctttagat
     9541 aaagcaagtg atccagtttt tgcacaaaag atgatgggtg aaggctactt tgtagaacca
     9601 gaaaacggac atatctattc tccagttact ggggtagtca gctctgtctt cccaacgaaa
     9661 cacgctattg ggatcacaac gccaaatggt ttagaaatct tgcttcatat gggaatcaac
     9721 acagttgact taggcggtac gccatttgat gtgaaagtaa cagaagggca gcaagtgaca
     9781 cctgatacgc ttgtagcaga agctgactta gaagcgatca aagcagcagg caaagaaaca
     9841 tccatgatcg tcttggtaac caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca
     9901 ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag cttaatatga tagaaacaat
     9961 agaaacgaaa gaagaaactg aaatctattt ttcagcttct tcttttttat tatttattgt
    10021 tttctgctaa aatctgataa attcgtgtca catcagatgg tgttccccgt aattcataat
    10081 ctgccaagaa aggaaagcca aatgtttgtg catattgttt ggctgttaga caatactgat
    10141 tattgaagtt tttatttccg ctaccgacta caccaagaca aagtttatga ttgtcttcat
    10201 gttccagata ttcacgcatc gtttcggtca agatttctgt atcaccgtta tctaaaccat
    10261 ttccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat
    10321 cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct tctgcatgca
    10381 ttttttcagc atattctgct agatgtttgg caaaggcacg cgtatttcct gaaatcgata
    10441 tatataaaat gttcatggat catcctccaa ttctttcctt ttagtatagc atctttttta
    10501 agttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata atgagaaaaa
    10561 agccggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat aaaaaaggtt
    10621 tgttatactt tttgataaaa gcggttttat tttggaggat gcttacatgg atacagagca
    10681 acagaaaaaa ggaattggtt taattccact tgctgcctta gtaattggtt cagcgattgg
    10741 aggtggcgta tttggtatta tgacggacat ctccggatcg gctggcggcc cagctatttt
    10801 cagttgggta ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa
    10861 taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag gttttggccg
    10921 tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag gcaacgtagc
    10981 ttttgcaaca ttgttgatga gcgcattagg atatttcttc ccagtatttg aaggtgggca
    11041 aaatctggca tcgatcgtga tgagtacagt ctttttatgg ggatatgcat ggctagtgaa
    11101 tcggggagtc gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta aattagtgcc
    11161 attattttta tttattgtag tagctatcag cacgttttcg atccaacaat tcattgataa
    11221 cttttctagc gacattatat tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt
    11281 attgagttgc atcatggtga tgctttgggt atttgttggt attgaaggag cctctgtcgt
    11341 aggttcacga gcaaaaaaac aatcagatgt tgggaaagca acgattctag ggatattcgg
    11401 cttgattatt atttacgtct tggtttccat gcttccatat gggacaatga cgatgaatca
    11461 gctgcaaacg atcaaggcgc agcctgctat gggctatgtg tttgaaatga tggttggtaa
    11521 atggggagca gtcgtgatca acggcggttt gatcatttct ttagttggtg tttggctctc
    11581 atggacgatc ttaccgattg aaacaatggg aaacatggct gaagacggac ttttgcctaa
    11641 agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat tgacgacgtt
    11701 atgtacgaat gtctttttac tttcattatt attcactgac agtgcttata actttgctta
    11761 cacacttggt acagcagcta ttttcttcac gtggcttttc ttaggattgt accaaatgaa
    11821 actttcttat cagcggaaag aatgggtcca atttttagta gggcttttag caagtgcctt
    11881 tcaagtatgg gctatgctag tcgtagcctt taatgaagta atgcttgttc ttgttctttt
    11941 cttgccaggc atttattttt acctcaaagc acgtaaggag caaaacgagc ctgtacaaca
    12001 tatccactta gataagattt tacttatagg tgtgacgatg gtcggagtga ttgcaatggt
    12061 tttatttgcc actgggatgc ttcacgtata gaagtataaa agaagtataa agagaggttg
    12121 tgagcctgtc gtttattcaa aaacatacta ggaaaggagc tgtgacacaa atgttgtcac
    12181 agctcctttt tcagaataaa cggtgtttaa gagacagcca atcggcaata aggctgaacg
    12241 tctctttcac agcctctttt ttgatgagtc atcatacatt ttttcgctta aatacgagat
    12301 atcctaatgc taagaacaaa acgatgtaag ctaaattacc gaatagcatt tgattagtag
    12361 aaagatgagt caattgcttc atgatctctt cgtttccaat ttggttttgc agattcaaca
    12421 tactgatcgg attccatttg atccactccc atttttggat gagtgcaata aggataccag
    12481 atatgattga cgaagcaaaa taaaagacga ttcctgctga gatggatgcg ccggaactat
    12541 tgataaagca agcaatcatc aacactaaac ttaaaatcag ccataaaccg atataactac
    12601 ccaaagtata agtgaacaag ctttggatca gtgtttgacc agtagaaacc ttctccgtga
    12661 aggaaatcga agggaaaaga atcagtttca tcaacacagt gacaataata gctaaaaggt
    12721 ataaataaac agaataaata actaatgtga tccatttgct tactaatatt tctcctcgag
    12781 aatactttcg ataaaggaga tttttcaatg taccatattg tgcttccatc gaaatgatcg
    12841 tgcttgcagc tgcaatcatg ataaagacaa tccaagatgt ggcactaaaa agctgagcag
    12901 tcatttcaac tggatcaatg atcgtcgtgt attttttggc tagaagtgct gttcctatga
    12961 gcaaaacaac taatagaaca gataaaacag cagttgactt tttcttgatc aatttaaata
    13021 attcttgttt tacaagcaat gtcatttttc ttcttccccc tttactgaat ccagaatttc
    13081 caatagagaa gattccaaat cattcgtcag atgctggacg tctgtcacat ggatattttg
    13141 tgcagacaat agctggatca cggttcctaa taattcttcg ttttctttta aaagcttgat
    13201 tttatcagga tcttccaaag tatatccagc ttctgtcaag atcgttcgag cagctagatc
    13261 atttgttgta gagataacga tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca
    13321 ttgtttgatg atctttcctt gattgatcag gatcagctca tcgaccattt tttcaagctc
    13381 gctgagtaag tgactggaga tcaagaaaga ggtgccctga gtagccaagt cggtaatgat
    13441 ttttcgcaca tctcttgttg cttgtggatc caaaccgttc atcggctcat ctaaaataac
    13501 gatagacgga tggttcagta aggcaagagc aattcccaat ttttgtttca ttcccaacga
    13561 gtaggatttc gcttttttgt gaatgtactc ttccatcttg agctgattca cgatagtgtc
    13621 gatagcagat acatcgttgg tttcagagaa taacttcaga tgatcatagc ctgttaagaa
    13681 tggatagata cccgggtatt cgatcaatgc tcctacttgt tccaaccctt tatgactaga
    13741 gggagaaatc tcattttcac caatacagat tgttccatct gagtaatgga tcagtcctaa
    13801 aatagctttc atgatcgttg tttttccagc gccattagga cctactaatc caacgatttt
    13861 gccaggggat aagctaaaac tgacatcgct gagggcttgt ttgtgaccaa agttcttgga
    13921 taattcttgg accgaaagac ttttcaaaaa tattcactcc ttttctatag atagtacaag
    13981 catacacctg atgagtggag aagtcgctag gtcattggac ttatttctta ttttttcata
    14041 aaaaaattga atattaaata aaaaggctta ttttttctga tcagtactta ttccaagtgt
    14101 ctagtggccg ctagtaatct tgatgccaat gatactgatg agtagcatcc ccacaaagaa
    14161 ccaagtggaa agattcagct tatcatgaaa caagaccaca ccaattatga ccgaaccaac
    14221 tgcgccaatc cctgtccaga taggataaga gagactcatc ggcaaacttt tgattgcctt
    14281 tgacaaaaag taaaaactag caatcatgcc aatcactgtg taaaaagaat aattgatttt
    14341 agaaaaacca tcactccatt tcatcatcgt tgaccagaat acttccaaaa ttcctgctat
    14401 gattaattcc atccatgcca tacttacatt cctccttaac tataaaaaaa tacacaaaaa
    14461 gaggaaaatc atcccttcaa agacctaatg gaatgatcat cctctttatc ctacccggtg
    14521 gcagattgta tatctttttc tgtagtatag cacacctatc cagatatcgg caatctcata
    14581 ctttttagta aagtgtacca agcactgacg gatatatcgt tagcggaaaa gaagatgaat
    14641 gctttgcaat ttcatagaaa gaagttgtta tttattaatc atttaaaaaa aacagtaact
    14701 agaccttatt tctatatatt atcgggcaat aaattaaaaa aagctttacc ttacaataag
    14761 taagataatt tttgtgataa aaaaataacg aaaaatattt aaaaatagtg tctatttcgt
    14821 gagaaaacaa cta
//
LOCUS       DACEOE010000059        14722 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEOE010000059 DACEOE010000000
VERSION     DACEOE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.061"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..561)
                     /locus_tag="IW774_001460"
     CDS             complement(<1..561)
                     /locus_tag="IW774_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153811.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVT"
     gene            complement(856..1191)
                     /locus_tag="IW774_001461"
     CDS             complement(856..1191)
                     /locus_tag="IW774_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1153812.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1178..1462)
                     /locus_tag="IW774_001462"
     CDS             complement(1178..1462)
                     /locus_tag="IW774_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1153813.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1518..3041)
                     /locus_tag="IW774_001463"
     CDS             complement(1518..3041)
                     /locus_tag="IW774_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1153814.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3034..4209)
                     /locus_tag="IW774_001464"
     CDS             complement(3034..4209)
                     /locus_tag="IW774_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1153815.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4213..4398)
                     /locus_tag="IW774_001465"
     CDS             complement(4213..4398)
                     /locus_tag="IW774_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153816.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4364..6058)
                     /locus_tag="IW774_001466"
     CDS             complement(4364..6058)
                     /locus_tag="IW774_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1153817.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6055..6528)
                     /locus_tag="IW774_001467"
     CDS             complement(6055..6528)
                     /locus_tag="IW774_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ1153818.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6597..6752)
                     /locus_tag="IW774_001468"
     CDS             complement(6597..6752)
                     /locus_tag="IW774_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ1153819.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(6886..7266)
                     /locus_tag="IW774_001469"
     CDS             complement(6886..7266)
                     /locus_tag="IW774_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1153820.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7270..7485)
                     /locus_tag="IW774_001470"
     CDS             complement(7270..7485)
                     /locus_tag="IW774_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153821.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7488..7895)
                     /locus_tag="IW774_001471"
     CDS             complement(7488..7895)
                     /locus_tag="IW774_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ1153822.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8161..9636)
                     /locus_tag="IW774_001472"
     CDS             complement(8161..9636)
                     /locus_tag="IW774_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ1153823.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9626..10474)
                     /locus_tag="IW774_001473"
     CDS             complement(9626..10474)
                     /locus_tag="IW774_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ1153824.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10511..10873)
                     /locus_tag="IW774_001474"
     CDS             complement(10511..10873)
                     /locus_tag="IW774_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153825.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11173..11797)
                     /locus_tag="IW774_001475"
                     /pseudo
     CDS             complement(11173..11797)
                     /locus_tag="IW774_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(11794..12105)
                     /locus_tag="IW774_001476"
     CDS             complement(11794..12105)
                     /locus_tag="IW774_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153826.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12149..12442)
                     /locus_tag="IW774_001477"
     CDS             complement(12149..12442)
                     /locus_tag="IW774_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153827.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12636..13283
                     /locus_tag="IW774_001478"
     CDS             12636..13283
                     /locus_tag="IW774_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1153828.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13344..14489
                     /locus_tag="IW774_001479"
     CDS             13344..14489
                     /locus_tag="IW774_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1153829.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14570..>14722)
                     /locus_tag="IW774_001480"
     CDS             complement(14570..>14722)
                     /locus_tag="IW774_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1153830.1"
                     /translation="VSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTF
                     MAEEEE"
BASE COUNT         4216 a         3050 c         2337 g         5119 t
ORIGIN      
        1 cgtcactttt tgcatcgacg aatgttttct ttcccaagat aacgtaaata gatttgactc
       61 tggacttata aagtctactt caccttctag tattactttc ttatgcaaat attcctctaa
      121 taaatataga cttgctaaag catacaacac attttccaaa tttgcttcct tataatttga
      181 agaacgacca tgttttactc tgttatatat tttccaccaa tctggtgtat cttcaggcca
      241 cccatctcca aaaggtttta gtgaagtcaa tgttaaagaa ttcataaccc taacaccatt
      301 atccaaacac attaattcgc tagatttaat aacagcttta aaatcagtta tgcttttatt
      361 gctactgcta tcaaatccgc aaagttcttt gaaaataatc tcacactccg aaccaatcgc
      421 tagtagtagt ttttgatatt caatagagaa tgttttgaaa ttattctcat cgagcatcac
      481 aaaatctgtg gtattaataa actcattttc taaatgtaga taatacttcc aataaaaatt
      541 tgtaaactct gctttgtcca ttaaatcacc tcttttgaat tataacaaaa aaaacacgtc
      601 cacaacagac gtgttctttg atttctacac aatcagcttg ccaaaaccat ccaatcagtc
      661 ctagcatgcc tgtattaaaa ttgaacgttc atcatctatc atctttctta gtccagcatg
      721 tttgcaacca cacctacaat tttcagtcca acaatagatt tcgactagct aagtcttgca
      781 aaattcaggc agctaaccct tcatttacta ccttaattat actaaatacc cacaacgttt
      841 acaagtgtaa tcattctaag aaatctgttt agctatgatc gttgtataat ctttaaacgc
      901 cgttccttca ttgattgaaa taattttata actcttgttt ttccaagcta ctttcatatc
      961 gtttgttagt tcataatctt gatcgtatcg aataataaac gtcaacgttc cttcaagaat
     1021 ggtacctaca ctagcaagca cgtcttttag catttgtgaa cgcacattag cccaacattc
     1081 aaatacagtt tcttcactat ccaccatctg cccatcttca tcttttaccc gttttgtctt
     1141 tttaaacttg actctctcag tcaattcatt tacattctta atgattggca taataagcac
     1201 ctctcatttg ttgtactaat gcttgtaccg ttactggtat ccgttcgctg ctggcttctt
     1261 gacggttcaa ataccaatgt tgagtaagta atgacaccgc caaatcaaat tgctgataat
     1321 tatccatatc tttatccgtt gcgctactat caatagcact ttttatatat tcgcctgctg
     1381 tatcaatcaa ttgttgaata aagtcatcat caatagtgtg atcgatccgc atactttttt
     1441 ttatgctatc caaaattacc attttatctc tcctaatgaa aaaaaggcag tgggataaac
     1501 cgccgcccaa tatttttcta tacttttggt gttacaactg gcgtaaactc aatatacact
     1561 gaagcatttt gatcaatttt cttataatcg ttgcgaacga tcactgcaag tccttgtgag
     1621 tagtaatcga atttttccca ttgagtcgtt acttgatttc tacgagcaac aaagatagat
     1681 tgtgcaatat cccccataat catagggaat gttccagctt taggattggc aaacaatgta
     1741 tctgcaatca atactactgg cataccgaat aatgatttac cactaggtgc tgtcacatcg
     1801 ggttgtaaaa tgtaacgtcc atctgaatct ttcaatgtat ctaggtggta atatccgctt
     1861 tggtttagaa ttaccatttt atttaatgct gggtctaatg ccacattgta tagttgtttc
     1921 aaatcgtcca acgtagcagc cgttttctta gtgaatgttt ttaataaatc catgatatgc
     1981 ttattgtcag tgttttctac caatttcgct aactgatctt tgacctcttg tacaatattc
     2041 actgctgaat cttccacaac ctcatttgat aaggcaatct taccagcacg agtttctact
     2101 ttataatcaa ctgaagtaaa catttcagcg tcaatatcac caatttcagc aagttcagct
     2161 tttgttgcta acactgcttg ttggttagtt gctactggat atttaccttg tccatttgat
     2221 actgttttta ctgttgcata ttgagctaag ttataatttg aacgtttcaa atcaaagact
     2281 tcaccgatta cttcttcggg aacaactgcc gctgcattta cagtagtgac tccatcacgc
     2341 acttcgcctt gtgaacggat atattcttca tacccacgca attctgtttt ttcgttatcg
     2401 attaaagttt tttccatgtt attactctcc ttttgtgctt tagaagtgga ttctaagcta
     2461 ttgtttgttt gttttgcttg gttgttactc ataaacgatt cgtaagaacg cttatttact
     2521 tggacatttg atgaatcata agcgggaacg gtcacgacac taatttcatt aagtgctttg
     2581 attttatcta ttgatcgttc aattgtgcca tcttcttttt tgtcgaatga atctaatcca
     2641 agcacgaacc caaaggacat agaatcaaca accccttttg agatattctc atacacatca
     2701 tttgaatagg tcgtgtcggt tagctctgct tcaaaatgta atcctgtatc atcgacattc
     2761 aatttcaacg tacctgcttt gacactagct agtggctttg aataatcgtg tccatataat
     2821 aagaacacat ttgataaatc cacctctttt aatgcttcgg gtgtaatcac ttctacaaag
     2881 ccgcctaaat cttttgacgg ttgcccgaat ttcaacgcat agcctgaaat agttttgccg
     2941 tctgttggtt gtttttcatt ttctttctct actttgggag aatcggctgt tagctcagct
     3001 tcttctgtca gccgtttttc cttttcttct tcattattca actggtgtaa ctccttcctg
     3061 ttcttttaat tgatatttct ccaacgtatc taaatacgta aagttcaaac ttgctagtaa
     3121 acgatcgcca ccatccatgg ggggtagccc cattttcgac cgcccctcat taatcgtgag
     3181 aagtgaccct ttgacctgat ccaatacatt ttttactttt gtttcaggat cagtctctag
     3241 taactgatcg gaattaaagc ggatattggt ttcaagtttc gtatctaatt cactcacaaa
     3301 cacattaaag taatgaatca gcgtactttg gatatactgg agattacttt gaacggtgct
     3361 agaatgttca ttttcaacgc ctaaacgctc aatcggtaca ccaaacgcct tggcaatttg
     3421 ttttgtgttc caatcattgg aattgataag cttcaaaaca tcggtgttta tttctaatgt
     3481 tttgtagtcc attgtttcat caagaatgat cgttctaaga gcattatctc cactacttga
     3541 accgttggct tcttcaaact tttcacgaat tgcgttcttt gctgatccgt ctaaatctga
     3601 cttatgaacc ttcaaaatac ctgacccact gacaccacga gtaaagaaat tgtgcaatgt
     3661 gcggttgcca gcttgttgaa ttttcagttc atcacgtaac gcataaagtg gtggtaatcc
     3721 tgttaaacca tcttgggtga aatatttgaa gtgcaaaata tcgctagact tcacacgtct
     3781 ttttttatca ccaatctgat aagacaacgt accattatct aattgagtaa ccgttacact
     3841 tgagttaggt aataagtgaa tttcttctac cttttcaccg ttgcgtttaa tctctgcaaa
     3901 gctattaccg tttagcaaca tattgaccgc caaagcaaac tttaaatgcc aaccatccat
     3961 ttctgaatta ggtttctcgt ttagtaactt caccagttca ttatcagctt gtggcatatt
     4021 gtttttgacc aattgaatcg gacttgaagc aatatcacta gcaataattc gaacggctgc
     4081 aaacacatca ctatttctta atgcacgaac gcttgtaaag ctggtggtgt aatcatccga
     4141 ttggattgat accacatgtt gtaggaaagg atctcccgtt gtattttgtc tcgctttaaa
     4201 aaatgccatc ttttaacctc ccttctgttc tgtatttagc atgaccgcta ccccaataag
     4261 cactatgccc agcgctaaca agccatagta gacatttgtt agtaaggtga tagcaaccaa
     4321 cacacacacc aatccaagaa gtaaaagaac ggtatgaatg taattagaaa gaaaattctt
     4381 cacttgtata aaattcattg tctgctttcg ctccttccgt tgtggtgaag taatccattg
     4441 caaacacata ggcattgatt aatgcagcta ttggatcaat tttgttactg ttctttgatt
     4501 tattaatctg aatgccgttg ttatccgttt ttaaaatagc gttgttcact gcatgggtga
     4561 gaatcgtatt tttattgtga ataacgttgc cttcataaac ttgttctcta aaagttcggg
     4621 tcggaacgtt tagagtaatc gttccttgtc ttacttctaa cattgggtag ttggcttttt
     4681 cagctttact gataagcgaa ttagcgttat acgggtcata acaaatcccc atacattcca
     4741 aatcattttc ttgaatcaaa tcttgaataa actgaaatac ttggtcatag tccacgattc
     4801 cactttctaa ttgagtaatg gaacactcac cagctttttc tagttctcga taaggcaaac
     4861 cgtcgcgctt ttctttgtct tgaagcccat atttggtggc tacaaagcta tgtgaatcac
     4921 aataaagttc gccgttatct agcggtacga tccatgaaac gctagttaaa tcatctgttt
     4981 tagataaatc cactccgata taaacgggct tgcctgtaat atcttgtggt tctacttcgg
     5041 ttgcctgcca gtcttcagca atcatatagc tgtcctcact cgcttgtctc cataaattga
     5101 agtttttcac caatacagaa ttaaggttat tttgtttcaa agcaaggttc acatcatctt
     5161 gaatggttgg aatcattact tttttgattt cttcactttc aaaaatcgga ttggctttga
     5221 tccatttctc ttgatcgtga atttcttcgg gatcgtccaa ttcccatatc gctataaagt
     5281 acctgtcggc ttgttcttcg ccttttagca cacgatctaa caataagtat tcctcgtaca
     5341 taggaacgtt taaatcaagc ccagcggtac taataacaca caataagccg ttcttttgtt
     5401 gggtcattcc tgacttaatg acgttgtacg ttttacgagt ttttgcttcg tgccactcgt
     5461 caagaattgc ggttgttggt gcaaacccat ctagcgtgct ggtttcacta gccagtgcca
     5521 tagcgaacga attagagggc aagtgctgaa tcttagagtt cataattttc agttgtggtc
     5581 ttaaaaactt actagacttg accacgtttc taagtgaatt agacagcata tcatagccca
     5641 atttagcttg tttcaaagcg ttacttacaa acaatacttg ccttgcttct gctggttctt
     5701 gttccataat caacgcattg gcagccatgc cacttgcgat atacgttttc ccattcttac
     5761 ggctcatact ggtaaacgct cgattgaatc gcctgtaatt cccttcttta gtacgccagc
     5821 cataaagact gcctagaatg aatttttgaa atcctaacat cttgattgct ttaccgtccg
     5881 tagatggtag caactcaatg aatttaatcg ctttggtggc ttgttcttca tcgaacacat
     5941 aagggaagct atctgactta gaacgctcca aatcgcttat atgacgctca cacgcttgga
     6001 taattttctt gggtgcaatc agcttacctg ataggacttt ctcaatgtaa tcattcatga
     6061 attcatcaac tctttgaacg ggtcttcggg ttcatcattt ttaaccttgt tcaaagctag
     6121 ttttgatcgt gattctaagg tcatacctag tgaggtggct tgttttttca aatcactcat
     6181 agcttgtgat tgaacccgaa ccgctggatt ggctttcttt actccgtttt cgcatgtgat
     6241 aaacgtacct gattttctta tctcggcttc ggctgttttc attcgagcaa aggctaaaca
     6301 ataggctacc aatgaaccat aatctgcttc actcaatgga taatcttttt ttaatgttgg
     6361 tacgatacga ttccattcac ttctagcgct cgctgccatc cagtcagggg gcgtgatatt
     6421 tattaatggt tgctgcttga acaattcttc tctagcgtcc acacggattg ctttttcttc
     6481 acttgatata ttgccttttg tatcgtgtaa tattttaggg tgtcccatgt tgtcctcctt
     6541 tcagacaaaa gaaaaaggaa cgtaagcaat taagctcaca ttccttcgtt tttcgatcag
     6601 aatttttctt tatctaattt atctatataa atgatttttc cgttctgcat ttgaatggtc
     6661 acttgaccaa aatgaggtaa ctcaatggtg cgctgtttcc ctgttttatc agtaataaca
     6721 attgtatttt tcaataaatc tcctactttc attatatact ttttcacttg tgattacaag
     6781 tgtaatcgtt atttgtaatt acccttatgc cttttcacaa cggttatttg taccaaaatg
     6841 agtaggcatc gattgtcagt aagtttcaca ctatgccccc ttaactcaaa gggtactctt
     6901 ttctcgtctt tgtctttcat tggctgtctt ggaattatgg tgtgcatggc ataaagaacg
     6961 aaaattagtt tcatctaacc gtctattcca atcatctttt agttcaattt tgtgatctac
     7021 aacatcagct ttccttatca agccttcttc taggcaatcc tcacatactg gatgatttaa
     7081 ccgatacaag tatgaagcct ttcgccatgt tctactttga tagaattgaa aatatttacc
     7141 ttcgctttgc tttctttctt cacgcttaat tgccgtttgc tttgctttat gcttcccaca
     7201 atacttctgc ctataatcaa tcaatgtttt acatccagca tgattacatt gttttttaac
     7261 tgccatagat taccaaccaa agcgatctcg ttcgtcagaa tcatcttgtt cccattttga
     7321 tttgtattca atataaggcg aaactatctt caagcacgtt ccgtctttaa aattaacttt
     7381 agtgatttta tcttcctcaa aactaataaa gtctacatct tcttgagtgt ttgaatactc
     7441 aatgtgtttc tcaccatcga agtatctaat actttccata tgcataatta ttctacctcc
     7501 aataaattag ttatcaattc atcatctgtt agtagcaaag ccaagaactt atcatggatg
     7561 tttaaaaaca aattatcctc attcgatgta agctctcgtt gtctcgctcg atcatatagg
     7621 ttaagcagac aattaaattc atccattagc tcacctgtat catcatagtt gccactatct
     7681 accaatgatc ccactactag cgattgacac cacgctactg actcctgcag ccaatcaggt
     7741 tcttcttcca ttatctttaa tacttctgga tcaaatgtgg aataatcttc tgttaccaat
     7801 cccacactaa atgccagcgt gttcattaga aaccgatcca tttcatctgt atattgaaag
     7861 ttcggtgctt tgtttgttga aataatattg agcatttact ttcctccttg attcgtaaca
     7921 cgtggtaaca cctacgtaac aggtacccgt taccactcaa tcccaacggg gacaagggtt
     7981 ctagcgtttt ggtaacaggt aacgggtaaa atccagtaac attattcccc agcatattag
     8041 ctaactattt caaaagtttt aaggaaataa ggtgttaccc cgttaccaaa ttgctgtatc
     8101 ccttggggca gtaagggtaa actagtaacg ggctaggtgt tactgacccg ttactacccg
     8161 ttactttttg tactttccag ctcgattttt attttcatag gcatttgact tattgtaaaa
     8221 tcctcgtcgt ttatttcttt ttggcagttg cttccgttcc caaccaggaa tactgctaat
     8281 aatcaaggca atctttttag tttctgcatc tgatcgacca ttcaataaat cctttgcttg
     8341 tttatcaaaa acagcttcca aaatatcagc ggtgagtacg ctatcaacta aataaaactc
     8401 ttgttgcata gtagctccat tatagaaatg ctgtaaattt ttctcattac atttactgtc
     8461 aggatagttc tgataataag aacgtctcgc ccacgctgga gcttcttccc atttatacgg
     8521 gatttccatt tccaaatatt tagtgattgc ttctttaatt ggattctcca tcttggcatc
     8581 ctcttgatag tcttttgcaa tttctaagac ttctttatct tcatcgggac taaaatagat
     8641 tcgttgtcct ttatcaaaaa gcacctttgc ttcagctaac acttgaagaa aatattcatc
     8701 ctttgtttta aacggattca tctttggctt attcttacaa ggcaaaggat aaaaacggcg
     8761 gtttcctgta tcatctttca aatactgccc atcattcgac gttccaataa atacacattg
     8821 ccttgcccaa gctatgacat tccgaccata aggaggtcta attttatcga tcttggcact
     8881 gatgaagttt tttatctgtt caattttagt tttgttcatt gaagataatt ctccaagttc
     8941 aataattaca ttgccttgaa gctgcatata atcatctttc ttttcaccta gcgaatctaa
     9001 tgaatcggtg aaaaaatcat ccgtgtaaag taatgctgga acggtactct taccaatccc
     9061 ttgcggtcca tccaaaactg gaacgatctc aaactttatt cctggtttat agatacgtgc
     9121 gactgatccg actatccaac gttttgttac ttctcgtgtg taaaggttgt cgtctacccc
     9181 taaaaggtca ataaacagtg tttccagtct aggctgttga tcccatttaa ctttttcgat
     9241 acgttctttt actgggttga aaacattctt tctagccaca tttactactg ctttgtgtat
     9301 tttgttgtca tcaaaaacta cattgtagtt ttcttcaata tatgccgtta aggcatcctc
     9361 aaaactgtct tcccattctc cagcggtacg attgatccaa ggagaatctt ttaataggtg
     9421 aatgtatccg ctaaattcgt tataagcaat tgttccagct aaattgctat cattggttaa
     9481 aatcaattct gcattcacaa cactgttact tttaatctga tcgtctttat tgcatcttaa
     9541 tcgtgacatc caggttttca attcaatgat attgtcttca cgctcgctgg cttcgggaaa
     9601 gtctaaaata ctatctaatt tatgcttatg agccatgagc tttccccctc aaatcttttt
     9661 taatcatcga tcgaataatt tgattcactt ctttatcttt tagcggtggt tcattgtacg
     9721 catttgctgt ataaatcaat tctgctaaag tttgattatc tacaccgtgc catagtaacc
     9781 aaccgcataa cttagttaaa tgatcgtttc tactaccttc gccagcgcct tgaacaatca
     9841 tatctaattt ttttgctgtc cctgtttttg ctacgggtcg gtattgagtc tttatttctt
     9901 gcggtttttt tagcagtagg ttcactagcc aatcgggagc tggtgcaata ttatttagag
     9961 atacgttttc aataggttca tatgctctcc caacacttct tgaaataatc atagttggcg
    10021 ctattggaac tcctttagtg ataatatcaa tcccgcttaa acttttacct ttatcatcac
    10081 gcccgaagat atcagcaaat gcacctgttt cttgttggat aattacacca tcaggaattt
    10141 taaagaagaa atggattcct ttacctggtg tgatttccgt ataagtagaa ggaaatggat
    10201 catacattga agcaagtttt ttataattct caacgccgtt tcgtgtttca gaatgttgat
    10261 ctaggtcaac taccaccatt tgatgatctt caagcattaa cccaatattt ctagttgcat
    10321 cttccgtcca ccattcatta atttgattag aatcgttagt tgcatcattc caaccactag
    10381 agccttttat tggaatttta gttccaggag ataagggata aattgaccat cccgactttt
    10441 gtaattccgt tgctgcttga taattatttg ccaataaacc tctcctcctt tttcaaaata
    10501 ctggtataaa tcattcagac acctccatga gcttcaactg ctcaagttcg tatttcaacc
    10561 catcccgaac aaattcagca ccttttaatt gattctgata ctttacccat gtattaaatg
    10621 ttttcaccct tgcttcttgc atatcagtaa tgtttaacca tgcggttaaa atctgatttt
    10681 ttagctcgtt gatttctcga caaattattt tgtgttctgc atgttttttg agtacataca
    10741 aaactgttag tttttcattg aatgaatttc caaagtaggt tttcccgata ttattttcta
    10801 gttcagcgat cccttgatta atttcctcta attcaaggct atcgagttgg tttaatagtt
    10861 ttgttttgta catctactta tgccctccag ttcttaattg atagtcgtac tcgtcataag
    10921 cagtgaagag atataacgct aatcctgcaa agatagctat ttttgcaatt aatggtaata
    10981 agccgaaaat tccgccgatt aaaatgcttc ctataattaa gtttcgtttc ataatggttc
    11041 cctccgattt tttaatatgg tataatcgga acaaagaata tcgggtttta tcccaattaa
    11101 gttgccttta tcctgtccgc caagactgtt atggataagg ctttttcttt gttctcgatc
    11161 atgttaatgc tcctaagttg tctgcaattc agcagaacga ttataaattt tctcactcac
    11221 tgaaaactgc aacccatatt tttctttaac agtcattaat tctactgtct catctaaaat
    11281 aggttcacga tcaaccaaca tttcaggagt catatctttc ttttttacca ttttggaatg
    11341 accataaata gttgatacag ctttattggc tatcacattc gctttaatgt agtctactgg
    11401 ttttggttca gtaatagcgt ggcttaattt agtcattgct tctttttggt gttctttgtc
    11461 tagcatgcgg aatacttgaa agccttcaag tcctgtggac tggcgtagtt ctttgatgat
    11521 gtcgaaaatc cagtcttgaa atgcctcggc ttctttttta cggcttttga atgccatgcg
    11581 gtaaatattt tttttcgttg atgatgttta catcttgtgt tctgcccaat gaatcgatga
    11641 ccttactagt agtaacccca tctttaggca aagagtgaat ggctctagta acctgtttaa
    11701 gacctaacgc ttccgcaaca tctttcgcca ctgcccacca ttcatcatta atatcgacaa
    11761 atcttataat gtgtccgttc caaatttcag ttttcattca acgtcctcca gttcaagccc
    11821 tagcaagctc gctaacaact caacttgctg atacaaaatt tcttgatact cttcaataga
    11881 aagtcgttct ttaaactctg cttgtactaa atcgttcaag ccacgcaata ctccgcttac
    11941 cgttggttct tgtggtagca atttttgata gtccatttgt tcgtacaaac attcaaacgt
    12001 ttcttctagc gctttgcctt ccaagtttgc taattctgcc gctgtgtctt ctgtaagaat
    12061 tgttgataat actttttcga ttgctttatt ttgtgtcgat tccattatta tttcctccta
    12121 ttaatccctt tgcccccacg gcttgcgttt atttttggta ttcttccata aagcgatcac
    12181 attcatcttg tgaaattctt acgactccat caactataat caactttaaa ccgttaggaa
    12241 catatttctg catcatagta ttgtatgaaa cgttcatata cttagcagct tgtgccttgt
    12301 tcatatattt tggaaactgg tacttttttt ctaataatga aagtaacgtt ccttgagttt
    12361 gctctacttg ttgaacaact cgatcgtcaa taattttaaa agctggatca tcaagcgtga
    12421 gtttgtcaat ttttaattcc atatttatcc cccctcgtta gcatacattt ctatacaaag
    12481 attttaaaaa aatgcaagag gctcttgaat acatttctat acctcttata aactcaatat
    12541 accatcatac cttttcattt gcaagaattt taacatactt ttttgaactt ttatgtttca
    12601 cacatattta aatcgtgata tagtagagaa aatatatgaa tggtggtgaa aatgtggcta
    12661 gaaaaaaata ccccgaagca gtggagacag gacaaagaat tagactggct agaattgaaa
    12721 agggactctc aatggaacaa cttggaggta aattatcccc accggccagt aaaggtgccg
    12781 ttagtaattg ggaaaatggc tataatcttc ccaataacac caggcttaaa caactttcaa
    12841 aggtattaga tgtatccact acctatctat taaacggatc ttatacatta agagatatgc
    12901 atatgatgcc cgaaattaag cgaaatgtgc ttgaaaagca attgaaaaaa actattgaaa
    12961 aaaataactc tataattgat tctaatcttt ctaatgctat caataacatc gaagaagata
    13021 acctgactgt ctcacagaag atttatttaa caaacgcaat aaaatatctt aaaacaggat
    13081 ctcgaattga atttcctgac gggagtaccc caattgctta tctcgcagct cttctaaata
    13141 cgcttttacg atatgactca ttaattaatg attcagaaaa aaatacagat gagaaaaaag
    13201 agtatcaaga attttatgaa aatgatcttc taaaagagtt cacagctctt gttactgagc
    13261 taaataaaca ctattcaaaa taataataaa ctgcctcctc caaccgcccc cacggcttgc
    13321 tacactatgg agggaaaact attatggcaa cattcaagca atatgaaact aaaaaaggta
    13381 aattttggct ctatgaagcc tatcttggaa tgaacaaaat gactggcaaa cctgataagg
    13441 ttcgtagacg aggttacaaa acaaaaaaag aagcccagct tgccttgtca cgcttgcaag
    13501 tggactacga taaaaacggt attaaaaaag cgaacaacga aacgtttaaa gaagtttatg
    13561 acttgtggct agaaacatat aaaaccactg tccgagaagc caccttcatg aaaactgaaa
    13621 tcaagtttag aaagtggatt cttccaaaat atggaaatct tcgtgtgaat gaagtcacgg
    13681 taaaaaaagc ccaacaaatt gtaaatagct gggctgaaac tactgaccaa tacaaggtac
    13741 ttcattcaac ttctaagcgt atttttagct acgctatcaa tcttgggatt attgagacta
    13801 atccactaga caacgtatta atgcctaaaa gaaccaccac taagcataaa gaaaaggtca
    13861 aagtttacag taaagaacaa ctgatcacct tgtttgatta cctaaacagc aaacctgaaa
    13921 cttatcgaaa cgactttgat aaaacgttac tacgctttct cttttatagc ggggttagga
    13981 tcagtgaagc ccttgcattg aattggtcag acattgattt tcaagaaaat acggtcacca
    14041 tcaataaaac actaagccaa tccaaaaatg gctataaaat cagtgatcct aaaactgatt
    14101 gtagtgctgg ggttcttcca ctagatgaaa gaacaatttc tatattaaaa aaatggcaga
    14161 ttaatcagcg gaaatatatg ctgacacttg gtgtaactga tccgactatg attttttgtg
    14221 gtatctataa agaactaatc acacaccacg ctatttatgc aagattgatt actatttgtg
    14281 aaaaagctat ggttccattt ctcggtgtgc atgtagcaag acacactcat gcttcaatgc
    14341 tcttggacgc tggagctact atggtagaag ttcaaactag attacggcat acaaaaatta
    14401 gtcagacgat cgattgttac ggtcacttgg caaaagaaac gaaagaaaag actgttgaaa
    14461 aattagtaca acacttaaat attgactaga tggcatacaa tatggcatac agtaaaaaat
    14521 aaaaaaagga gaaaccctat catatcaagg tttctcctag ctttatagtc tattcttctt
    14581 cttccgccat gaatgtattg aatacttctt caatcatgtc ccattctgcg tctgtttcga
    14641 tttgttctaa ctcgccttct gttccatctt cgttttcgat gtatgcataa gcttgtaatt
    14701 ctacatcttc atcttctgaa ac
//
LOCUS       DACEOE010000060        14493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEOE010000060 DACEOE010000000
VERSION     DACEOE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.062"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..94
                     /locus_tag="IW774_001481"
     CDS             <1..94
                     /locus_tag="IW774_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1153831.1"
                     /translation="VKKTVIDPIDEKEAMIEENRKKAMRKRVSR"
     gene            complement(156..1967)
                     /locus_tag="IW774_001482"
     CDS             complement(156..1967)
                     /locus_tag="IW774_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1153832.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1964..2329)
                     /locus_tag="IW774_001483"
     CDS             complement(1964..2329)
                     /locus_tag="IW774_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1153833.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2492..2887)
                     /locus_tag="IW774_001484"
     CDS             complement(2492..2887)
                     /locus_tag="IW774_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1153834.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2905..3867)
                     /locus_tag="IW774_001485"
     CDS             complement(2905..3867)
                     /locus_tag="IW774_001485"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1153835.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3867..4079)
                     /locus_tag="IW774_001486"
     CDS             complement(3867..4079)
                     /locus_tag="IW774_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1153836.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4100..4798)
                     /locus_tag="IW774_001487"
     CDS             complement(4100..4798)
                     /locus_tag="IW774_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1153837.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4818..5360)
                     /locus_tag="IW774_001488"
     CDS             complement(4818..5360)
                     /locus_tag="IW774_001488"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1153838.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5492..6496)
                     /locus_tag="IW774_001489"
     CDS             complement(5492..6496)
                     /locus_tag="IW774_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1153839.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6493..7482)
                     /locus_tag="IW774_001490"
     CDS             complement(6493..7482)
                     /locus_tag="IW774_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1153840.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7479..8285)
                     /locus_tag="IW774_001491"
     CDS             complement(7479..8285)
                     /locus_tag="IW774_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153841.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8451..9407
                     /locus_tag="IW774_001492"
     CDS             8451..9407
                     /locus_tag="IW774_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1153842.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9484..10002)
                     /locus_tag="IW774_001493"
     CDS             complement(9484..10002)
                     /locus_tag="IW774_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1153843.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10090..10239)
                     /gene="rpmG"
                     /locus_tag="IW774_001494"
     CDS             complement(10090..10239)
                     /gene="rpmG"
                     /locus_tag="IW774_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1153844.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10467..10913
                     /locus_tag="IW774_001495"
     CDS             10467..10913
                     /locus_tag="IW774_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1153845.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11108..13003)
                     /gene="asnB"
                     /locus_tag="IW774_001496"
     CDS             complement(11108..13003)
                     /gene="asnB"
                     /locus_tag="IW774_001496"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1153846.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13328..14302)
                     /locus_tag="IW774_001497"
     CDS             complement(13328..14302)
                     /locus_tag="IW774_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1153847.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         4530 a         3044 c         2439 g         4480 t
ORIGIN      
        1 tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa gcaatgatcg aagaaaaccg
       61 taaaaaagca atgcgtaaac gcgtttctcg ttaataatta tttaaaaata cgcatgaaga
      121 ctttctaaaa aacagataag tcatcatgcg tatttttatt tgtcagtcaa tataagttgt
      181 gctattttat ctgcctgtct cagattccat ggatctgtat aaggtctagc tactgctgca
      241 gttagccaat ccacaccttc aacaggtatt ccatagcaat aaaagtcaga ctggatttcc
      301 cctgaagcat caatcacgcg attcgtacct cgctcaacat ctatagcttc tgaatgataa
      361 cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt caaatttttt
      421 atgacaggat tacttgaacg agaaaagctt gtttgtggca atcgagcttc aatcaaatag
      481 cgactgctga tttctttatc ttgtagagaa ataacatagc ggttgtgctt ttgctgaata
      541 tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta tcagtgctgc taattctttc
      601 gtccgaatga ttggcggacc gatcgttaaa aagctattta gatgagtaaa ccatccccat
      661 agtatttctt tgtattcctt agcagaaaaa acgtgccatt caatagcctg ccggattgga
      721 tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca attgcctttt
      781 tccgcttctt ttatctgccc ttcaataaat attcggctag cttctgaaaa gggctcattc
      841 tgataggcaa gaggagtttc taaatacgac caatcccatc tataagggat tagtgctgga
      901 tgctttccga gacactcctc tgaagagagg gagagaaaat catgctcaaa atttttcttt
      961 ggaatcggca agtgatattc ttcgatgatt ttcttgtaat aaaacaactc aacgtctttt
     1021 ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca ttgctccagg
     1081 ttttcttttg ttagtaaccg tggccatgcc attgctccgc cttgcttttg atttcttcct
     1141 cgagggtaat aagggagccc ccttcttgat ccactgtata caatcggttc cttgcctgat
     1201 ggatgataga ccagttttcc cttttcgttc tcgaagcgcc ctcctcttga tactgtaaac
     1261 aatccgacat aatcaaaaaa acttaagcct agacctctta aaaagacatt ttctttaggc
     1321 ggtatcttcg aaagatctac atctgcagga ttagaaggaa gctgatacag taaaacattt
     1381 ttctttgcgt agtctaccca cactctttct tcttctgtct catcattttc ccagtgaccg
     1441 cttgctaaaa caagctgatc gatagaatag ctttgctgat ctgtctgtaa aagataacca
     1501 tcttttcttt tgcttacttt ttttactaaa gaataaacaa actgaataga agctggaaat
     1561 tcttcttgca actttgagaa aaaccagcgt tgatagagtc cgtataaaca tcgagtagat
     1621 tgctgatctt tttccagacg attgatttct tctataatta gttgcttatt tttggttacc
     1681 gtctgttcga tataatctgt agcaggtcct ttactccatt gaaacaagtt gggtccctgt
     1741 gttaccaatc ctccactgga caatgtttca tctgtaaaaa gcgtcacttg ttgagaaaca
     1801 gaattcatta ataactccgg tggctgattc aaccgccaaa ctcgacctcc aggaccattg
     1861 ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat tattatggag aatcctttct
     1921 gctacactta atcctctagg tccggcacca atgatcccta ttttcatttg catgcctcca
     1981 tttcgataaa ttttttcaat aaaacagcat ggttctgatg ttcatttttt gcaccataaa
     2041 caaaagtaat gactggatgt tctttcataa gttgactgag tttaaaaata gcttgcttct
     2101 tttcttcttc gaaaatcaat tcttgttggt atttctttgc aaatgaagca aatcgttctg
     2161 gtacatggtc aaaagttttg cgtgcttcat tactaggtgc aatttctttc aaccacagat
     2221 ctaactgcgc tttttctttt gaaattcctc gtggccagaa tctatccacg agtattctat
     2281 agccatcctc tttttcagca tcaaggtaaa cacgttttaa tttgatcata gttgtttcct
     2341 tctttctacc ttttcgctta gcttatatat gtagataata gcacaaaata aaaaaaatga
     2401 acggcacaac tatccattgc gccgttcatg tgatcttggt caaaatctaa tgattgattc
     2461 ggtttttaaa gctgtcatac aatcatctgt tttatacttt tttacttttg atttttccag
     2521 accatccgtc atatccaccc ttaagtaaat aaatctcttg ataacctgct ttacgtaatt
     2581 tatttgctgt acggatgctc aaacttttct tttgatcgta aatatagaca ggctggtctt
     2641 ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt gcacctaaaa
     2701 tatgcccagc atcaaacaca tctttttcgc gaacatcgat tacttgtgct tttctcattc
     2761 cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg tttggccatt actcgtagat
     2821 agacttccca aaggatgatt gcaagcacaa tcaatagcaa aatgatatta attacccata
     2881 aaaccatgta atgcctccgt ttttttaagc aaatttcaag gctaatgagg cagcaccaat
     2941 gacaccagct tcattaccaa gctctgctaa tttgattttt gttgattctc ttacttgcgg
     3001 gaaagtaaat tctttaaaat atttctctac acggctacgt aaaaattctc ctgctgccga
     3061 tactcctcca cctaacacga tacttgacgg atttaatgta ttccccaagt ttccgcaagc
     3121 taaacctaag tagaagcata cgcgatcaac aaccatcaat gcaaacggat cgtcttcttg
     3181 agccatttca aagatatctt tacttgttac ttcttctccg ttgtctagca ttgctttcag
     3241 ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct aggcgtacca cacctgtagc
     3301 gctagataca gtttctaaac atccgcgttt accgcaagta cattcgaacc cttcaggatc
     3361 cacagtgata tgtccgattt ctcctccagc tccgctcacg ccgtgaatca aatttccgcc
     3421 agcaacgata cctccgccaa cacctgttcc tagagtaacg aatacaacgt ctggatcgtt
     3481 ttctccagca cctttccatc gttcacctaa agcagcaaca ttcgcgtcat tatcgatagc
     3541 gaatttgatt cctgttcctg cctcgatcag ttttttcaca aattgaactt ctgtccagtt
     3601 taagttatac gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg gggtacccat
     3661 gccgattccg ataaagtctt cggctttcat attatataaa ttcaaacgat gattgattga
     3721 ttcaatgatt tcaggaacga tatgcttacc atcatccaaa atattggtat cgatgctcca
     3781 tttttgctga atctctcctt ctggtgttaa aattgcaaat tttgctgttg ttccacctaa
     3841 atcaatccca ataatcttct tgtccattac aaatttcctt tctttcggct ttcttcaatt
     3901 cggtgctctc tttttaatat gcgccacgct gtcatgtatg tatcatgatc aatcaaacgg
     3961 ctatcataca gattacgcaa ttcgatagtc attaattcaa tgtcatagat gcgtgcaccc
     4021 acatagataa agatgccaaa ttttttgaac agctgctgta catcatataa gctctccatc
     4081 tttaagcact tccttcatat tatacaagta aaccatattt ttttaaaccc agtaaaatac
     4141 atattacaaa aagaaataca aaaactagtg tagaaagaat cctttggtgt atcgaatagc
     4201 ttgtctgttg tttcggtaga cccaaaacat tccctaacac taatccgcca attagcccac
     4261 cgacatgtcc ccaaacatct acagaggtat ccagaagacc aaaaacaaat gtcaacacaa
     4321 taaataaaag aaaatgtctg actagctgct ggatcgctgg gttatgttta aaatgaaacc
     4381 ctaagataaa caacgcaccg aacaaaccga atatcgaagt acttgcacca ccagacagaa
     4441 cattcgcttc gttaaaagca aagcttgctg tatttcccat tatcccgctg aacatataaa
     4501 tcaagaaaaa acgccaatgg ccatagattg cctcgatttg ctgccccata aaatacagta
     4561 tcagtgagtt cattacgaaa tgcatcagtc caaaatggat gaagattggt gtcactaaac
     4621 gccaccattc attgaagtga acaagataag gaccatacat tccggcataa taagggatct
     4681 caagtcgcgg aaataattcc atcatgagga agaccgctgt ctgtatccct aaaaaagcat
     4741 aggtccataa cggtcgtctt aaccatgcac gcaattttat ttgctgttga taattcatga
     4801 aaacctccca tttcatttta gtcaataaac aattgttgga tcttctgatc aaagctctcc
     4861 ggttgccatt gttcattcaa ttgttcacga aaaacaaggc tacatgtttt tttatgaaaa
     4921 ttatttaaat accggtcgta ataaccaccg ccaaaaccga ttcgataacc ggctctattg
     4981 tagataatac ctggtacaat gaggagatcg atcatctccg gcaaatagag cttctctgat
     5041 tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg actgttcatc catttcataa
     5101 aacgccatct gacgtttcgg caaagtttta ggtacaacta ttttcttttg ttctttttct
     5161 gctcgttgga taaccggctg tgtatccagt tcaaattcat tcgataaagt cgtccctatc
     5221 acttttgctg acttccagta ctcgctggcg aatagctgtt tatagattcg ctgttctttt
     5281 tcctttttct tgccactttt ggccagattt ttcagctttt cgattccttt ccggcgtaat
     5341 cttactttca attggtccaa aaactcaccc ttccttctct cataaaatgg tataggaaaa
     5401 aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa tagaataaaa agaagaccta
     5461 gaaatgattg gccttccttt tttaatgaat ttcaactgtt cagcgcgtgt ttttgtctga
     5521 taaaaagtgc atacagaaaa tatggcgcac caaaaagggc aatcaaaatt cctgtaggta
     5581 cttccccact tagtagaatc actctagcaa ccatatctgc actaagcaga aaaatggaac
     5641 cgaacaaacc gctcagaaca aaggtccagt tattttcttt acgtacgact agcttcgcag
     5701 catgaggagc tagtagtcct acaaaagata aactacctgt caccgctaca ctaccgcttg
     5761 ccaaagcaac agctaacaac aaaaggattt tttgactttt agccacgtta aggcctaatc
     5821 cttgcgcgcg ttcttgacca aatgatagca atacaatcct ctgtgcataa agtcctccta
     5881 gcggaattaa gataaccgcc catggaagca atgcccaaac ataaggccag ctcgcacccc
     5941 agattgatcc cgctaaccat gcattcacaa atccataatt ttccttagat aattggatcg
     6001 tgcatagcaa tgttaatgca gaaaggccgg cattaacggc aataccggac aataagattc
     6061 gattcatacc caaaaagcca tttcgatcat aggccgcaag atagacacag caagcagcta
     6121 aaacaccacc tagaaaggct acaactagca atagaaacgg cagcgaatag tgcgaaaaga
     6181 aacctaaata aagcatcact aaaaatcctg ctccggcatt aatgcccaat atactagaat
     6241 ctgccaattc atttcttgtt actccttgca agatataacc agaaacgcca agacaagctc
     6301 ctgctaataa actgatcagt aatcgaggaa ctcgaaattc tctaacaatc agcgccaaag
     6361 aagatgattc tttccctatg aacgtggcaa aaagctgatc caggtaaata aaaggcgtac
     6421 caatcattaa actacctata gacaggatga caagtacaaa aagcaagaaa tagaacgtcc
     6481 aatatatttt tttcaccgtt gttccctcct tacttgtatc agtaaaaacg gaacgccaat
     6541 caatgcaatg actaatccaa aaggtgtttc aaaaggggga ttgatcatcc tcgctgtaaa
     6601 atccgccaac ataaccaatc caccgccaac taaaaaagag acaggtagaa cataacgata
     6661 gtccgttcca accattccgc gaacaactgg cggaaccaac agcccaataa atgaaacagg
     6721 tccgactaac gaaacggcaa tggctgctaa cactgcaact agacagaaaa caatcaaccg
     6781 cgttcgttta ggatgacttc caagagctaa tgcatgtgtt tcactcaaat gaatcaaaga
     6841 tattgctttt cctaatgaaa aagaaaccaa aacagcaagt atatataaag gaaacaatac
     6901 tgctaattgt ttccatgtga tattggcagc tccgcctaca aaccaaaaag caagatcctg
     6961 ctgcagatta aagaattggg taagtgcttg gctgatagca gtaaaaaaag aactaatagc
     7021 tacgccagct aaaatcagct gtaaaggatt catgcccatt ttcgaaaact gcaaaaaacc
     7081 aaatacgagc aaagttacag caaaagaacc tgccaatgac attagaaaca cagaaaaatc
     7141 agaagcttta ggccaaaaag caaaactaag ggccattgcc aacgaagcac ccgcgttgat
     7201 tcctagtaag cctgaatctg ctaaaggatt tcttgtcact ccttgcatca atgcaccgct
     7261 caatgcaaaa caactcccta caagaaatgc tcccgccaca cgaggcaaac gaatatttcg
     7321 tatgatttgg tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc aaaaatctgc
     7381 ttttcccaca gtcgctgcgc catttgaaag agcaacaaaa aaaataatca aaagaacaaa
     7441 acagaccaaa agcaggagtc tatttttctt catagtcctc ataattgatc ctcctgctct
     7501 tttggcaaca aatcataggt tagaaaaact ggtttatttg ttcttggaag cgtgacaatc
     7561 gttgcatcaa tctcaaatat ttttttcata cactcttttg tgatcacttc ttcaggtgta
     7621 ccttctgtga tcaacttacc gtttttcatt gctaataaat aatctgaaaa acgtgaagca
     7681 tgattaatgt catgaatcga catcaagatc gttgtttgtt tcatttgatt gatttcagtc
     7741 aataactgta ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg ttcatccaag
     7801 atcaagatat ctgtttcctg agcaagcgcc atagcgatcc agacacgctg ggtttgtcca
     7861 ccagacagcg ttgcgatatc ccgcatacga aatccagcta gacctgtagc atccaacgcc
     7921 cactcaattg cttcatgatc ttgcgtgtcc atgccactga acagtttttg ataagggtat
     7981 cgtccgtaag atactacttc ttctactgtc atacctgtag ggtgttcact agtttgtgct
     8041 aataatgcaa ttttttttgc aagttctttt gaactgtatg tagagatatc agagttattg
     8101 acaatgatct ttcctgattt cacaggtagt atacgggtaa aacttttcaa taaagtagat
     8161 tttccacttc catttggccc aatcaaacta gtgattttcc cttcaggaat cgtaacagac
     8221 atgttttcaa tgattttttt gttttcgtag ccggctgcaa tgtcagctgc tcttaatctc
     8281 gtcatttcac ccatcctttc gctgaaaatc attctcaatt atgacgctgt ttcaacattt
     8341 cgtcaagcgc tactttcata tctatagcca aatgaagcgg ttgacagcgt tcgaaatatc
     8401 aggaataata accaatgaga aagattctca attatgttag gaggaaagaa atgaaattaa
     8461 aaacaaaagt attcacatta tctagcatat tgctgctggc tggagtactg agcgcttgct
     8521 ccccctcttc agataaagaa acagctacaa ctgccaattc gacgacttca tccaatagta
     8581 ctcatacagt cacagataca cttggacata aagtagagat tccaaacgcg cccaaacgga
     8641 tcatcggcag ttacttagaa gactatctga ttgcactagg ggaaaaacct atcgcccagt
     8701 ggactgttgg cagtggttcc attcaagatt atctgcaaga tgatttaaag gatgtcccaa
     8761 cgatttctta tgaccttcct tatgagaaag tgttaagctt tgagcctgat ttgctgttga
     8821 tttcttcttc cgctactgtc gaaggcggga aatacgaaca atatagcaaa atcgcgccaa
     8881 cttatgttgt aaaaaacgga acagatgtca catgggaaga acaactaaaa gatgtcggca
     8941 aagctttggc aaaagaaaaa gaggcagaaa aaatcatttc cgattatcaa aaatccatca
     9001 agaaaactcg agaagaacta gcagataaaa tcgagaacaa gacagctgca gtgttgtggg
     9061 tgaccaacaa ctcagccttt atggtcagca aaaatcggtc aagtggaaga atcgtatacg
     9121 acgatctgga atttggtgtt cctgatctag tggaagaggt ctccaaaaaa gccacttctg
     9181 actggtctgc tgtttcctct gaaaagctag ctgaattaga tgctgactac atcatccttg
     9241 tgaacagtga caaagatgct gccatgttca atgaaccaag ctggaagaat ctaaaagcag
     9301 tgacagacaa tcatgtactg caattcggac ctgaatctag ttggctatat aacggaccta
     9361 ttgccagtca ggaaatggtc aatgatatca aagaaaaact aaattgattt taactaaaaa
     9421 aagcatcttc acgattagtc gtgagatgct ttttttctta ttaacaaact agacgcatag
     9481 attttattct tcttttgaag caaccctatt ttttactctt tcccattttt gaaaacgatg
     9541 ggcatataga ttttcttgat ccgttatccc ctcatactcc tctataaggg cgaagtctga
     9601 aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca atgttcgcca
     9661 gatccgacca gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg aaacatagat
     9721 atcttctgag atcgtttgac tatacgacaa aagttcatcg atcgaatgca ttacttccgc
     9781 attttctttc gtgcgaaaat ttggttgcgt tgtcaacacg atgatatggc gataaggaag
     9841 tgaaagatcc cccattcctt cataggtctt tcttcccatc actaaaattc gatccattgt
     9901 gtgttcgcga aagaaacgca tatcatttgg caatcgccat ggtaacaaac catttttgcc
     9961 gatcagacca tttttatctt gtgcccacat ggaaataaac atattatccg ctccttatga
    10021 ttttagatgt tttgagataa aaaaagtctc aattctttga ccagcaagga attgagaact
    10081 ttaaaaacat tattttgttt cacggtgcaa agtaactttg cgttcgcgtg ggcaatattt
    10141 ttgtttttcc aaacgatcag gattgttacg tttgtttttg cttgttagat agttacgttc
    10201 tttacaagaa gtacactcta aagtgatgtt tacgcgcatg tttttccctc ccatttttac
    10261 aagaatcctc taagatgact ctcagacttg attatcttac catgattttt tctagattgc
    10321 tagtgttttt cacgattttg ttaagtaaaa ctacttgacg ataaatacaa cggttttact
    10381 tctcaaacaa aatctttttc tttttgtata aataccctta cacttgttta tatagattat
    10441 gttttttaat aaagggggat actaatatgc catggaacat gaaggacttt cctgcatcaa
    10501 tgaaaaactt agataaattg acaagaaaaa aagcaatcga catcgcaaat gcattattag
    10561 atgaagggta ccctgataac cgtgcgatcc caattgccat tgatcaagca aaaaaatggt
    10621 atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa agaaaaaaat ccaagcaaga
    10681 cggatgaaca cgatacgaat cctcgtgctg gaaaactgct tgattcagat gtgatcgtag
    10741 aatatgagga agaacaatgg atcgtgaaat ctaaaggggc aaaaaaggca agcaaccatt
    10801 ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt agcacaaaac aaagaatcaa
    10861 ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga aatcgattat tgaacatatg
    10921 taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat gttcgaaaca atggtgttcc
    10981 tctgccaact ccgaaccata agtcataata tctataaaac gggaagagct gttttttgaa
    11041 attgtttctt atcactcagt gttaaacggt aggagaaaac ctctttttta ttgatatttg
    11101 attttcatca aacagtcaca tagtcgattg cttctgcttg cggaatcgtt tcatctacaa
    11161 agtagacttt ataccatgtc aagaaactga agattgtcca gatttttctt tgctcttctt
    11221 tacgtccttc gtgatggtca tctagcaact tcatgatttt ttcttgatca aaaaattctt
    11281 ttgcaaaatc tgcttcgaat aatgctttga cttgttcgta gccgtgttct tctttcaacc
    11341 aagctttgat tggcacaggg aatcctaatt tcacacgatt cgaccattct tccggaagat
    11401 gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt ttgattcaat aaaaacttac
    11461 ttggtatttg ttgagccaat ttcatgactt cgatatccaa taaaggtact cggacttcca
    11521 aagaacttgc catagaaagt ttatctgcct ttaacaagat atcttttggc atccattgat
    11581 gaagatcgac atattgcatc ttattgactt catctttgat tccttctgtt ttctcgtaat
    11641 gaacagtcat gatttcatcc acagatggtg ctgtttgata ttttggtgtt aacacttctg
    11701 atgcttctga ttcttcgaac actcgagcat gaccgataaa atattctttt gcaggagcaa
    11761 gtgattcata taaatggatc cgtccatgga aatttttcat ttttccgatt ttacgtccta
    11821 aattatagcg tgttcctttt ggtaattttt tcaatccttg agcaatcaca cggatgaatt
    11881 ttgaattcgt atggaatcca tacgtttggt atcccgcaaa cagttcatcc gccccttctc
    11941 cagattggac tacccgcaca ctttgtgaag caagatttgc taaaaagtac aaaggtacgc
    12001 aagaaggatt tgaatcaggt tcatctaagt gatactggat caaagggaat gctttgaacg
    12061 acatgtctcc gtcgatcact gctgctgtat tatctaagtc caatagttcg gttagttttc
    12121 tggcttcaat cgcttcatta taggttttat catcaaaccc gattgagaaa gaatgatctg
    12181 gtttcaatac agaggttaca taactggaat caaccccgct ggataagaaa gaaccaactt
    12241 ctacatcact gaccgtatgt gcttcaatag aagcttgaac ggtttcgtcg attttttcga
    12301 tccattcttg tcgggaatgc gtttcttttg tttcaaaatc tgcatcccaa tattgctgaa
    12361 tgtctatttt tccatcttta tacgtataat aatggccttc aggcaaacga tagacatctt
    12421 tgaagaatgt ttcgccgttt aatggagaat attggaaagt catataaggt ttcaacgctt
    12481 ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa acttttgatt tctgaaccaa
    12541 acataaatgt tccgttcatt tctgcataat aatatggttt gatcccaaag tggtctcttg
    12601 caccgaataa ctcattcttt tcattatccc aaatagcaaa agcaaacatt ccgcggatct
    12661 tttgcaataa ttctgttccc cattcttcgt aaccatgcaa aaggacttct gtatctgcat
    12721 gtgtttgaaa aacgtggcca gctgcaatca attcttctct caaaggctgg taattataga
    12781 tttcgccatt aaaaataatg atctttgtcc gatcttcatt gtaaataggc tgagtaccgc
    12841 cctctaagtc aataatactt aatcttctga atcctaaagc aacatgttta tcgatatatt
    12901 ctcctgaact attaggtcca cggtgaacga ttcgatccat cattgtatta ataattgttt
    12961 ttttgttgtc cttgtcgtta acaaaaccta caataccgca catttaactt tcacatccta
    13021 atataaaatt actgtttatt tcattcatat gcatcttatc atagaataaa tagcggacga
    13081 atattttctc tcttacaatt tcgtcacgtg aaaacaaaag atgaaagttt ctgtttttac
    13141 agaaaataaa gtcccaatat ctaatttacc atgttttctt ttttgaggct accttattct
    13201 cttcgtgtta aaaaaatatt agtggatgct atttccaaaa aaaccagcaa aaaaagacac
    13261 gacctaaaga tcgtgtctaa ttatgactgt atcagaaaat atgataccaa acggatctat
    13321 tcattaatta attgacatag tttatctgtt gctcttttat gactggataa ctgaccaatt
    13381 catgacgatc caagtcttct ttattcatat ttacagcagt aatttgactg tcttcatacg
    13441 tacgataata ataaatccct ttatcaacgt tacagcaaga agaataaatc gtatattcgt
    13501 attttctttc tccagtatcg catactcctt tttgttgctc gactgagcct agtatatgaa
    13561 aaaattgact gatactttca gattctgaat cacctgatgc agcattcatt ttagtaaatg
    13621 tggcttttac aaatcgagaa acagaagaga gatctccagg taagcccaat cctcccatcc
    13681 cacgactata aacattcaag ctgatctggt ttgaaaaatg attttctggt gttccatttg
    13741 atagcgagcg ataattgttc agattaaata attgataatc aaatggcgga ttatttgtca
    13801 acacaccgac aggattatca tataaatgaa gtccatcttt catactttca attactatcg
    13861 atttttcttt atcagctaat aaccagtgta aaggtgacaa gggtagtttt tcactaaaat
    13921 cgatattgac tagattgata gtagccaata attctttagc ttcgtctatg gttgagcatt
    13981 gccctagtat ccatggaata aactcaaatg gtgcaacgtt atcttttcct tcttgtaatt
    14041 ctttataatc agcattgcca ggaaaattca gtcctgccat actcaaccct ttttcatttg
    14101 tagcctcata atagagtgga tagttggata ccccagctgc tattcctatc attgcataat
    14161 gtttatctaa attttttacc tttctaaaat cgaaacgata atttcttgga gtaatagtga
    14221 ccacttcatt ataagacatc tcataatcaa aattccttcc aaaataatgg tctttcgttg
    14281 cataagtgat tgcggtacac atcgtttccc gtcctaaaac aaatcgtttt tacactttta
    14341 tcttacatct agtatttttt ttatcaaaaa aaggccaaag aagattgatt tactagcatt
    14401 tataaaaaat agtactcagg gggaatcaaa aaatattgta ggttaaaagt acaacaataa
    14461 ggggctgtga caaaagtctt gtcacagccc ctt
//
LOCUS       DACEOE010000061        13816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEOE010000061 DACEOE010000000
VERSION     DACEOE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.063"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(159..1469)
                     /locus_tag="IW774_001498"
     CDS             complement(159..1469)
                     /locus_tag="IW774_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1153848.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IW774_001499"
     CDS             complement(1481..2899)
                     /locus_tag="IW774_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1153849.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IW774_001500"
     CDS             complement(3139..4707)
                     /locus_tag="IW774_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1153850.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IW774_001501"
     CDS             complement(4711..5121)
                     /locus_tag="IW774_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1153851.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IW774_001502"
     CDS             complement(5118..6041)
                     /locus_tag="IW774_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1153852.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IW774_001503"
     CDS             complement(6038..6916)
                     /locus_tag="IW774_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1153853.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IW774_001504"
     CDS             complement(6903..8240)
                     /locus_tag="IW774_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1153854.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IW774_001505"
     CDS             complement(8243..9346)
                     /locus_tag="IW774_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1153855.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IW774_001506"
     CDS             complement(9420..10928)
                     /locus_tag="IW774_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1153856.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IW774_001507"
     CDS             complement(10894..11586)
                     /locus_tag="IW774_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1153857.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IW774_001508"
     CDS             complement(11608..12582)
                     /locus_tag="IW774_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1153858.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IW774_001509"
     CDS             12882..13754
                     /locus_tag="IW774_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1153859.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
BASE COUNT         4088 a         2931 c         2278 g         4519 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaagg
//
LOCUS       DACEOE010000062        13506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEOE010000062 DACEOE010000000
VERSION     DACEOE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.064"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            62..763
                     /locus_tag="IW774_001510"
     CDS             62..763
                     /locus_tag="IW774_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1153860.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            760..1881
                     /locus_tag="IW774_001511"
     CDS             760..1881
                     /locus_tag="IW774_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1153861.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1896..3128
                     /gene="pepT"
                     /locus_tag="IW774_001512"
     CDS             1896..3128
                     /gene="pepT"
                     /locus_tag="IW774_001512"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1153862.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3260..4168
                     /locus_tag="IW774_001513"
     CDS             3260..4168
                     /locus_tag="IW774_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1153863.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4203..4475
                     /locus_tag="IW774_001514"
     CDS             4203..4475
                     /locus_tag="IW774_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1153864.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4486..5532
                     /locus_tag="IW774_001515"
     CDS             4486..5532
                     /locus_tag="IW774_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1153865.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5783..8392
                     /gene="clpB"
                     /locus_tag="IW774_001516"
     CDS             5783..8392
                     /gene="clpB"
                     /locus_tag="IW774_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1153866.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLIQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8543..9214)
                     /locus_tag="IW774_001517"
     CDS             complement(8543..9214)
                     /locus_tag="IW774_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153867.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9207..9701)
                     /locus_tag="IW774_001518"
     CDS             complement(9207..9701)
                     /locus_tag="IW774_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1153868.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9721..10941)
                     /locus_tag="IW774_001519"
     CDS             complement(9721..10941)
                     /locus_tag="IW774_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1153869.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11088..12923
                     /gene="lepA"
                     /locus_tag="IW774_001520"
     CDS             11088..12923
                     /gene="lepA"
                     /locus_tag="IW774_001520"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1153870.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13120..>13506
                     /locus_tag="IW774_001521"
     CDS             13120..>13506
                     /locus_tag="IW774_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1153871.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEK"
BASE COUNT         4475 a         2430 c         2807 g         3794 t
ORIGIN      
        1 ttattcctag tctccttatt ttatttggaa aatgctataa ttatctagaa ggagtgaagc
       61 tatgaaccat ttagatttat ctcagcgctt agccgcagtg ggtgactttg tccctgaatc
      121 cgctcgttta gcagacattg gatcagatca cgcttactta cccgttgcat tgatattaag
      181 aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa ggtccttatg aatcagcacg
      241 taagcaagtc aaaaaagatg gattagaaga ccgtatcacc gtacgccttg caaatggtct
      301 agatgctatc cagccacaag atggtattac agctgtcacg attgcgggta tgggaggttc
      361 actgatccga gatatcctag aagctggcaa gttgaatcaa cgcttaagcg gacaagaacg
      421 tttgatcctt cagccaaaca tcggagagcg tacattacga tcttggctgc aagaaaatca
      481 gtataaaatc caatcagaaa aaatacttga agaaaataaa aaaatatatg aaatcattgt
      541 agccgaaaaa gaacaaaaac cagttactta caatgaaatg gagctgatgt tcgggccatt
      601 tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg acaagagagc ttcaacaaag
      661 aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa gaggcacgtc aaaaacaaat
      721 cgagcaggaa atccaaatga ttgaggaggt tttaaagcca tgacaatcag cggacgcact
      781 ttcatccaaa agtttgaaag ctattgtcct cagtggctag ctgaagaagg agacccagtc
      841 ggactgcata tcggaacatt ggataagcca atccaacgag taatgatgac attagacgtt
      901 cgtccagaag tggtcgaaga agcaatcaag aaaaaaatcg atttgctgat tgctaaacat
      961 cctccaatct ttcgtcctgt aaaacggctg gttactgatc agccacaaga aaaaatgtat
     1021 gcagatttgt tgaaacatga tattgccgtc tatgcagcac acacgaacat ggatattatt
     1081 tgggatggac tgaatgactg gttttgtgaa cttctcggta ttgaagtgga gagttattta
     1141 gtaaagactc atgagattca ttataaaaaa ctggccgttt atgtacctgt ggatcatgca
     1201 cagaaaatgc gtgaagtatt agctgcagct ggtgctggta cacaaggtga ttataccggt
     1261 acaagtttta cctcgattgg acacggtcga tttactccgg aagctggtgc acaaccagcg
     1321 attgggaaag tagggaagac agaacaagtt caagaggcaa aagttgaggt tattttgccg
     1381 gaaactatcg aaaaacaagt gattcaagcc atgcggtcag ctcatcctta tgaggaacct
     1441 gcttatgatc ttttcgctat agatgaacca gtcgaaatgt ttgggttagg acgagtaggt
     1501 gaattgccgc aagaaatttc tatagaggca tttgtagaac aagtaaaaga agcgttccaa
     1561 ctagatggac tgagaatagt ccagccaaaa aatgcaaaat cctctgtgaa gcgcattgcg
     1621 atttgcggtg gaagcggtga aaaattttat ccgcaagcaa tcgcccaaag agcagatgtc
     1681 tatatcacgg gagatatcta ttatcatacg gctcatgata tgcaaagcgc tgggttgatt
     1741 gcaattgatc ccggtcatta tattgaatcg ttatgcaagc aacgatttat cgagaaattt
     1801 gaatcttgga aacaagaaga aaattgggat attaactttt tcgtttctga aacaaataca
     1861 aacccatttc aatttaatta aaaggagaga atactatgta cgaaaatctt ttgccacgtt
     1921 ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac aagcaaaacg actccatcga
     1981 ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga attagaagag ttaggcttag
     2041 ctgatgtttt ctataatgaa gataacggat tcgttatggg aacattacca agcaatgttg
     2101 atcatgaagt gcgttccatt ggatttattt cccacatgga tacagcagat tttaatgcag
     2161 taggagtcaa tccacaaatc attgaaaact atgatggcga gtctacgatc gtactagatg
     2221 cggaagaacg ctatacattg aatacaaaag attttccaaa tttgaaaaat tatgaaggcc
     2281 acacattgat cacaacagat ggcagtacat tactgggagc agatgacaaa tcagggattg
     2341 cggaaatcat gactgcaatg gagatcttga tcaataatcc ttctatccct cacggtacca
     2401 tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg agcggataaa ttcgatgttg
     2461 agcaattcga tgtagatttt gcttatacag tcgatggtgg tcctttaggt gagttgcagt
     2521 atgaaacgtt tagtgctgct caagcaaata ttacgatcca agggaaaaat gtacatccag
     2581 ggacggcaaa agatacaatg atcaatgctc tacagttagc aatcgatttt cataatcagc
     2641 ttccagccga tgaagttcca gaaaaaacgg aaggatacga aggtttcttc catttgatgg
     2701 cattggatgg gaatcctgaa gaagcttcta tgagttatat cattcgcgat catgatcgtg
     2761 aaaaattcga agcacgcaaa gcacaaatca cacatatcca agaagagttg aatcagcgat
     2821 ttgatcaaga acgaatcaaa gtagatatgt atgatcaata ttacaacatg aaagaaatca
     2881 ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc aatgatcgag cttggtatct
     2941 ctccagtgat cgaaccagtt cgtggaggaa cagatggttc aaaaatctct tacatgggta
     3001 ttccgacgcc aaatctattt gccggaggag aaaatatgca cggacgattc gaatttgttt
     3061 ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa aattgcagaa ttgaacacaa
     3121 agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt cgtcccaatg atggaatgtc
     3181 atctgtctaa ggctaaaggg caggacaatg gaattggttg tcccgccctc ttttttgaag
     3241 agtaaatagg aggtaaagaa tggaaaagct gctagttttt tataacaaaa gttcagggaa
     3301 agatgagggt gagaaattag cacgctggtt tcaagattat gtaaaagaaa agcagccaga
     3361 attatatgta gaactagctg aaactggccc atcgattgat gatgcggaat tagtcaagaa
     3421 agcagaagcc atagaagctg atactttagt aattataggt ggagacggca cgattcatca
     3481 tatcgtccaa gcgttccaaa aaacattatc tcaataccat gtcggattac ttccaggtgg
     3541 aactgtcaac aaccttgcac gtgttcttga gattcccctc aaaaaagaag aagctgctga
     3601 cgtgatcctg gaaggcaatg tacggaaaat agactatggt caagtcaatg atgaagtcat
     3661 tatctctaca ctgacaatcg gaatcttagc agatacagca gtttggatca gtcaagaaga
     3721 aaaacagcgt tacggctcgt ggatctttat caagaagttt tttaagttat tgctgaagaa
     3781 gaaaaaatat catttggatt tagaaacaga cgaggagcat tggaaaggaa aatcccaact
     3841 gttgacagtc acgatgtcta actccgtggg cggttttaca aattttgacg actcagcgac
     3901 cctagatgat ggaaaatttc atgtaacgat cgtaccttcc ttagatattt tccgatttat
     3961 tttttatttg cctagaatca tgagaggcaa attctatacg atccccggca ttacttattt
     4021 cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct gtgggcactc gaacggatgg
     4081 agacacgaca gatgatcttc cagttgaatt atctgttgta tcaaaaggat taacagttta
     4141 ttcaccaaag aaagaagaaa aagcttagaa atagtagtta acagaaagtt ggaagaagag
     4201 taatggatgt attgttacac aatcttttgt ggttttacct tttcattgtg aatttttact
     4261 tattttgtct gatggggtat gataaacatc aggctagaac tagcggctgg cgtgttcctg
     4321 agaataattt gatatttgtc agcctgttag gcggtggttt gggaggattg atctctcgaa
     4381 aagttttcca tcataaaact cggaaaaaga agtttactat aggattcgct gcaggtgtac
     4441 ttgttgattt gattctcatt tattttttcc attaaggagc aggagatgtc gtctaaagga
     4501 acgaaaacta aaatcattct gaatctgctt ttgctgattg tgatttttgc cattatttat
     4561 tatttaatca atcaatcatt tgcagatatc tttgccgaat tgctgtctac cagtgttgtt
     4621 gttttggcag cggctgttat attagggaca gtttaccaaa tcgctgaagg gcgctcaatc
     4681 aaagaaattg ctgaaccttt cgcgccgaat tttacaacga aagatgggtt cttgacctct
     4741 agctatattg cgttttaccg aatcgtttcg tttggtacag gaacgctgat ctcggagatt
     4801 tatttttata acaaaaaagg aatgaagcta tcccagggag caggggtgac agcactccat
     4861 atgattatgt acaaactggc agtgatcact tatgcattag ctggcttggt catccaattc
     4921 tctctttttt attctaatgg tccatcaatg atctggttta tcatcgctgg tatccttttg
     4981 acgatcgtga ttgttgcctt tttattggcg atgtccgtca gcttgaatgt tcaaatattt
     5041 tttgtcagct tatcgaataa gttatttaaa agtgagcgtt tgcggaaatg ggtggataca
     5101 tgcaatcagc agatctactc tttaagggaa acggtccaaa ccattatcca agacaggact
     5161 gcgctattgc ggatttatct atggaatatg ttaaaacttg tattttggta tgtacttcct
     5221 tatttgattc tgattgaaaa ccatcctaaa atagattttc tactgaccat ttcgtttatc
     5281 agctttgcag tagtgctttc aggggttatt cctacgcctg ctggtattgg ttcctttgaa
     5341 tttgtctatt tgttattatt cagaccagtt gttggaacag ttgacgcagt ttcttcgctt
     5401 ttactgtatc gtttcagttc ctttatcttg ccatttctct atggatttgt gtatgtgatt
     5461 gctgaacggc gaagagtgat caaagaagaa ataatagaag taaaaaaaga gaataaagaa
     5521 aatcaagggt aggtctttta taagatctac cctttttatc aaatcaaaga aaaacgttat
     5581 ggataagata attttacgtt tgtttggctc tttttttata tacatacaat caattttgtt
     5641 tttttaagat ttatataagt cgagaaaaaa agtcaaaaaa ccatgtgtat attttgacca
     5701 aaattgacca aagcgatata atagtttttg taaagatcaa agaaataagt gatcttacct
     5761 ttaattagga gggtatacct gtatgaatat tgagaaaatg actacgacgc ttcaagaagc
     5821 gattgctgaa gctcaacaga ttgcggtcac tcgaaaacat caagatatcg acattgccca
     5881 tgtatggaaa atctttttac aacctaatca ttttggtcga aatttctata cggatgccgg
     5941 attggatgtc gaatcttttg aacatgaaat tgatcgtcta ctggatgagt atccagttgt
     6001 ttctggtgga aacgtccaat atggacaaaa cctcagccag aatttctttc gattattgaa
     6061 tgaagcagac caaatacgtg aaagcttcgg cgacgagttt ctttcaacgg aagtcgttat
     6121 tttagcgttg atgaaattaa aaaattatcc gttaactgtt tatttgaata aaaacggatt
     6181 atctgaaaaa gaattgcgta aaaacatcga agagatgaga ggaggagaaa gagtgacctc
     6241 acaaaaccaa gaagaacaat acaaagcatt agaaaaatac ggcgttgatt taatacagca
     6301 agtcaaaagc ggaaaaatgg atccaatcat cggtcgtgat gaagaaatcc gtgatgtcat
     6361 ccgtattctt tcacgaaaaa caaaaaataa tcctgttctg ataggagaac ccggtgtagg
     6421 gaaaacggca atcgttgaag ggcttgctca acgtatcgta cgcaaagatg tgcctgaaaa
     6481 tttaaaagat aaaacgattt tttcattaga tatgggtgca ttgatcgctg gagcaaaatt
     6541 ccgtggtgaa tttgaagaaa gactaaaagc agtcttgaaa gaagtcaaaa aaagtgatgg
     6601 acgtatcctc ttgttcatcg atgaaatcca caatatcgta ggggcaggaa aaacagaagg
     6661 aagtatggat gccggaaatc tgttgaaacc aatgcttgct cgcggtgaat tacatttgat
     6721 cggtgccaca actttagatg agtaccgtca atatatggaa aaagacaaag cgttggaacg
     6781 tcgctttcaa aaggtattag tcaaagaacc aacagtggaa gatacgatca gtattctgcg
     6841 tggattaaaa gaacgttttg agatccacca tggtgttaac atccatgaca acgcattagt
     6901 agcagcagct actttatctg accgttatat tactgatcgg ttcttaccgg acaaagcaat
     6961 cgatttgatc gatgaagcca gtgcaacgat tcgggtagaa atgaactcta tgccaacgga
     7021 attagatcaa gttactagac gtttgatgca gttggaaatc gaagaagctg cactgaaaaa
     7081 agaatcagat gatgcaagta aaaaacgctt gaaaaatttg caagaagaat tagctgaact
     7141 tcgtgaagaa gcaaatgcga tgaagatgca gtgggaaaca gaaaaagaag aagtcaattc
     7201 tgtttcagca aaacgtgctg aaatcgataa agcaaaacac gaattagaag atgcagaaaa
     7261 taactatgat ttagaacgcg ctgctgtatt gcgtcatggt acgatacctc aattagaaaa
     7321 agaattgaag gaattagaag ctaaagcaaa agacagcgag attaagatgg ttcaagagtc
     7381 cgttaccgaa aatgagattg ctcaagtcgt tggcagatta acgggtatcc ccgtaacgaa
     7441 acttgtagaa ggtgaacgag aaaaactaat taaattgaat gaaacacttc ataaacgagt
     7501 gattggtcaa gatgaagcag ttgatgcggt cagtgacgcg gtaattcgtt cacgggctgg
     7561 attgcaagac ccaaatcgtc ctctaggttc tttccttttc ctaggaccaa ctggtgtcgg
     7621 aaaaaccgaa ttagcgaaag cgttagctga gaacttgttt gactcagaag atcatatggt
     7681 acggatcgat atgagtgaat atatggaaaa acatgctgtg tctcgtctag tcggagctcc
     7741 tccaggctat gtaggttacg aagaaggtgg tcaattaacg gaagctgtca gacgaaatcc
     7801 ttatacaatc gtcttgttgg atgaaatcga aaaagcgcat cccgatgtct tcaatatctt
     7861 gttacaagtc ctagatgacg gacgcttgac tgattcaaaa gggcgagtag tagatttcaa
     7921 aaatacggtt ctgatcatga ccagcaatat cggttcacag ttactgctgg aaggcgtgac
     7981 tgctgatgga acgattccag aagcagtggc tgaacaagtc aacacgctat tacgtggaaa
     8041 cttcaaacca gaattcttga accgaatcga cgacacgatt ttatttacac cattgagcct
     8101 agacaacgtg aaaggaatcg ttgacaaaat ggtggcgcaa ttggcacaac gtctggaaca
     8161 tcaagaaatc ctgctgacaa tcagtgatga agccaaaacg tggattgctg aaaatgctta
     8221 tgaaccagca tatggtgcaa gaccattgaa acgatttatc actaaggaag tagaaacacc
     8281 actagctaaa gaaatcgttg caggacatgt tatgcctaaa tcaaaagtaa cgattacttt
     8341 acttgatgga caacttcatt tcaaaacaga agagctagaa gaaatcgtct aatcttccat
     8401 tgagactata aaagaaaaaa ggggtctgac acaactagtg tcagacctcc ttttttcaaa
     8461 taaacagtgt tccagaagta gtttcttcga aaaataagcc gaaatttctt gaagctgccc
     8521 gcttctgtca caacctcttt tattaggtat ttttcacttt ttctaattta taactttcaa
     8581 agtaacgctc atctttttta ctttgagacc atgcaaacaa tcccattaag gcagcaccta
     8641 tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc tgctcgtccg acaaacagtg
     8701 tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa gaactggtcg cataagaatt
     8761 cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc aaagccgaat aagaggaaca
     8821 accaaggact ggtttttgat atctcagcat ctttcatcaa gtaaccaatc aatccatagc
     8881 caagagctgt caggaccatt ggactcaccg catgtaagat tttgtcccaa aaagagatga
     8941 tactgatcaa atgcatccct gtgcctaaaa agacagagat gaacaagaaa aaccagtaaa
     9001 acaaaacgat tgctgctggt attttaagtt tgaaaacttt caaaatgact tgtggcatga
     9061 aaatcaatgc aatccctaat aaagattcca cgataaatgt gattccttga gaagtcgaat
     9121 atttttgtgt gataaattca taaaaattat agcagacaac gaatacacca aaagcaagta
     9181 aagctatttg cacctttttg atttctttaa tcactgaaag tccctccatc taaaaatctt
     9241 tttacttctg aaagcaactc aatttctgaa gaaaccactt ttccgttcaa tttgatcaaa
     9301 cctattgtat ataagttcag gtagtgaaat tgattttcag ctgtttcttg tagtgcagcg
     9361 atttttttcg ggtgggttcc gccttgctgt cgagtatcag tatataagcc gatcactggg
     9421 atgcctttcg cataagccac accgatttcc gatgctaccc cggcatcgat cgtcagccca
     9481 tctagtaaag caatcattaa atcactagcc aacacttttt ctgtgtcagc aagagcaatc
     9541 attcgactgt ctgcataagc agttttgtca ttgattgctt cattttcttg aggaagatag
     9601 atcgaaagct cagaagagag ctctctgatt tctttaacta actgttggtt atataacaaa
     9661 tcacttttag caaacattgg tgcggcaaaa taaattttca tctaaacatc atccttttta
     9721 tcaaaaataa tcgctaaagt aagcttccat gtttggaatg atacgctcta gtgacttcag
     9781 tgtttcttca tctgtttcta accgttcgat tcttgccaaa taagaagcac ggcgataatt
     9841 catcatcaag caagtcaatg tttggatatc cagtcgaacg gattttccta taggttcatt
     9901 taagacacgg acttgtccct gttcatccca tgttaaagcg aagacaccat tattccaagg
     9961 agcgacagga tcttcaatga tgaaatgaaa agcatcagct gttccaacaa acggaaaccg
    10021 ttgaaggaat tcttttacat caacgatccg agccataaaa tatggttcga tctgctcttt
    10081 gatctgacta tcttcaatca aaaatgctaa aggctcattt ttataaatat ctccatgtac
    10141 ccagtagatc atggaaaaat gtgcggaaat aaagttccat aagccatttc gagcttcttg
    10201 atcaagatag aacatttctt ttacgtgaaa tatttcttca gctacccagt agaacagaac
    10261 acctcttggt tcatggttgc tatcgtagta gacagcggct gtgcgctcat cttcattttc
    10321 gaaacgccaa tactcttccc aatgaaaatc actgcggaat aaagcaccat ggttttgttg
    10381 ggcaaatttg tcataaacgg aaaatacgtc tggatgatca actggtaatc gctcaaccat
    10441 accacttact gggatttgtt taggtagctg tgtatctctg attttaaagg aaagtttatc
    10501 agacatgatt tcccatcctt tacgccgata gtaggggata ctgtaaggat acaaataaga
    10561 gatccattgc ttattcttgc gcatattatc caatgcaacg ataattagat cttgcatcaa
    10621 tccatggtta gcatattctg gataagttcc aactccagtc acaccaccca ttttataacg
    10681 tgcaccgtga atattgactt cacaaggata gatagcgatt tgagagatca gtttattttc
    10741 gtggaaccat ccgaacactt tcgatacttc caaaatcggt tttttggaac ggataaaagc
    10801 tcgtttattc tcaaacccgc tttcctcgat gtctgcttca gtcacttgga acacatagga
    10861 aagcaattca ttgaattgat cgatatgttc ttgagtgacc ggttttaatt caagattttc
    10921 tctaaaatct ttttgatcca ttttttcatc tccatttatt agcttgataa cagtatagca
    10981 aaattctaca aaaaaaactt ctatagctga ctaattaaaa actctttaaa ttactgcttt
    11041 tcgttggtat aatggaaagt gctgattata tgaaaagagg agacgctatg gatataaaga
    11101 aaatgaaaga acgccaagag cagattcgta atttttcgat catcgctcat atcgaccatg
    11161 ggaaatctac attggccgat cgtattttag aaatgacgca cactgtgacg tctcgcgaaa
    11221 tgcaagatca gctgttagac tcgatggatt tggaacgtga acgtggaatc acgattaaat
    11281 taaatgctgt agaactcaat tacacagcga aaaatggaga aacatatatc tttcacttga
    11341 ttgacacccc agggcacgtc gacttcacct atgaagtctc tcgaagcctt gctgcctgtg
    11401 agggtgccgt tttagtggtc gatgcagcac aaggaatcga agcacaaaca ttggccaatg
    11461 tttatttagc gttagacaat gatttggaga tcctgccagt catcaataaa atcgacctac
    11521 ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga cgtaatcgga atcgatgcta
    11581 gtgaagctgt attagccagt gccaaatcag ggattgggat tgaagatatt ctcgagcaga
    11641 tcgtggaata tgttcctgca ccaagtggag acttagatgc accgctaaaa gctttgatct
    11701 ttgactctat ttatgatagt taccgcggtg ttgttctaaa tgtccggatc acggacggta
    11761 tggtcaaacc cggagacaaa atcaaactga tgagtaatgg caaaacattt gatgttacag
    11821 aggtcggggt cttctcgcca aaagcagttg ctagagactt tttgatggtc ggtgatgtcg
    11881 gttatatcac agcaagtatc aaaactgtcc aagatacacg agtaggggat acagtaacgt
    11941 tagcagacaa tcctgccgct gaagctttac caggttaccg aaaaatgaat ccgatggttt
    12001 attgtggtct ttatccaatt gatacttctc gttacaacga tttgcgggaa gctttagaaa
    12061 aactgcaatt gaatgatgct gctttacaat tcgaaccaga aacctctcaa gcattaggtt
    12121 ttgggttccg ctgtggattc ctcggacttc ttcatatgga tgtcgtccaa gaacgcttgg
    12181 aacgggaatt caatctagaa ttgatcacga ctgcaccatc cgttatttac catgttaata
    12241 aaacggatgg tacgactgct acagtagata acccggcaga ttttcctgaa cctgtaacga
    12301 ttcaggatgt agaagaacca ttcgtcaaag cacagatcat ggtcccaaat gattttgtcg
    12361 gtgcagtaat ggaactttct caacgaaaac ggggagaatt cattacgatg gattatttag
    12421 atgattaccg agtaaatgtc gtctataata ttccgctttc tgaaattgtc tttgactttt
    12481 tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt ggattatgaa atgtctggtt
    12541 atcaaaaaag caagttagtg aagatggaca tcttgctgaa tggagaaaaa gtcgatgcat
    12601 taagctttat cgttcaccga gactttgcct atgaacgtgg aaaagcaatc gttgaaaaac
    12661 tgaaaaaact aatcccacgc caacaatttg aggtgccaat ccaagcagct attggacaaa
    12721 aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa cgtcttggct aaatgttatg
    12781 gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca aaaagaaggg aagaagcgga
    12841 tgaaacaaat cggctccgtc gaagttccgc aagaagcctt catggccgtt ctgaaaatgg
    12901 atgagcaaga tagtgtgaaa taacttataa tcaatcgaaa aacctattaa atcaaccttt
    12961 ttgagtagat gcaacactga ggattatctg tataaaagat tttaaactgt catttggtta
    13021 tcacggcgat actaaaatta acaaaattgg aatatttttt cttgaagatt tttagctgtc
    13081 agtatatgat gaaagcatct cataggaggt gcttttttca tggttgataa ttcaagtgga
    13141 agggttctta aatctctaag aaaagagaaa aaactatctc aaaaaaagtt agctgattta
    13201 gcaggtattt cacaaagtac tttagttaaa tatgaaaaag ggtcaagaaa aatccctaaa
    13261 gacgtagata atactctaag caagattttg aatatagaaa ctctaataaa agatgaggaa
    13321 gataagattg agattctcat cggtcaacta attgcataca gagatatgaa taagttactt
    13381 aacaaagagt tagctgataa tataggaatt agtgaagtat tactcagtta tgtgttaaat
    13441 agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg atatttttct attaagtaat
    13501 gaaaaa
//
LOCUS       DACEOE010000063        13296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEOE010000063 DACEOE010000000
VERSION     DACEOE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.065"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..661)
                     /locus_tag="IW774_001522"
     CDS             complement(5..661)
                     /locus_tag="IW774_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1153872.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            854..1132
                     /locus_tag="IW774_001523"
     CDS             854..1132
                     /locus_tag="IW774_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153873.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1151..2359
                     /locus_tag="IW774_001524"
     CDS             1151..2359
                     /locus_tag="IW774_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1153874.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2507..2872)
                     /locus_tag="IW774_001525"
     CDS             complement(2507..2872)
                     /locus_tag="IW774_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1153875.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3019..3792)
                     /locus_tag="IW774_001526"
     CDS             complement(3019..3792)
                     /locus_tag="IW774_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1153876.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3966..4826)
                     /locus_tag="IW774_001527"
     CDS             complement(3966..4826)
                     /locus_tag="IW774_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1153877.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4826..6244)
                     /locus_tag="IW774_001528"
     CDS             complement(4826..6244)
                     /locus_tag="IW774_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1153878.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6301..6750)
                     /locus_tag="IW774_001529"
     CDS             complement(6301..6750)
                     /locus_tag="IW774_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1153879.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7195..7458)
                     /locus_tag="IW774_001530"
     CDS             complement(7195..7458)
                     /locus_tag="IW774_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1153880.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7557..8852)
                     /locus_tag="IW774_001531"
     CDS             complement(7557..8852)
                     /locus_tag="IW774_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1153881.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8849..10120)
                     /locus_tag="IW774_001532"
     CDS             complement(8849..10120)
                     /locus_tag="IW774_001532"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1153882.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10285..11154)
                     /locus_tag="IW774_001533"
     CDS             complement(10285..11154)
                     /locus_tag="IW774_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1153883.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11446..13056)
                     /locus_tag="IW774_001534"
     CDS             complement(11446..13056)
                     /locus_tag="IW774_001534"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1153884.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3731 a         2814 c         2291 g         4460 t
ORIGIN      
        1 ttcatcaaaa gataatattg tcttctaaca agtccatatt caaaataacg atatgatggt
       61 ctttaacggt gataatacca tcttttttca gttgttttaa aattctgttt acactgctag
      121 cagttgagat gccacaaaat ttggcgattt cttcattcgt gatcgaaaaa tcgatcagca
      181 tcccttcttc tgtccgcaca ccgaataaat catataattc gtaaagttgc gtacaaacag
      241 ctcccagctt tccgtttaat agcatttgct gcattttttt catcgaataa agcagacgat
      301 agcggtaata ctcttttaca tagtgttgta gctttgggtc ttggttgata ttttgccaaa
      361 agcgcacacg attgattcga taaaactctg catattctga ttcaactcga atattatacg
      421 gcgaatcgat gaattgtgaa tattcgtctt ttaaaagaga gacgatttca agatctttga
      481 tatagcgaag gttaaattct ctcccatcat ttgaaataac acttgctttt accgttccct
      541 ctttcaagat atacgtatgg gaatcttcca tcccttcgaa tgtcaaatat ttatgatatc
      601 ctttgcgtat aatcggaaaa tcatttttat ccaaatattc ctttaagact ctactatcca
      661 atgatgctcc cccatccaaa ctttcatcta aaaagatcat acagtacttt aaaacttttt
      721 aaagtccaaa ggtattttac taatgatatt aacatttgtt aagtaccttt ttccatcact
      781 tatgcgattt ttttcctttt ttttattcgt tatactgcag aagtaaaatt ttttaagaat
      841 agagggaaaa aacatgaatt actggatgaa cacaatcatt aatcgattag aaacagcata
      901 tcaaacaaga tttgatatga aagcttcttt agtcttttta aatgatgcgt atcaaaactc
      961 gatcgaattg atcaaagcag tggatgagaa tccaacaaac gaatgcgaag aatttttaaa
     1021 tctgtttatg tctacaagag atttatttat tcgccaactt gttgatcgct atccatccaa
     1081 ttatcacgat gtagaagttc aaatacaaaa attaaaagca tattcagctt aaggggaatg
     1141 tataacatac atggaaaaga aattatttaa taaatatttt attggaatca cattgatcaa
     1201 ttttattgtc tacctgattt attatctgtt gatggtcatc atggcagtca ttgcgcaaga
     1261 caccttacac gccagcttga gccaagcagg ttttgcttct gggatctata tcatcggaac
     1321 attgctggca cgcttattca tggggaaaca gttagaacta tttggtcgga agtttacctt
     1381 aagaggcggc gcgatcttct atctcctttc aacattagca tatctttata cgccaactat
     1441 cggggcattg tatttagttc gattcttgaa tggattcggc tacggtacag tttctacagc
     1501 tacaaatgct atcgttactg catatatccc tgcatctaaa aaaggggaag gcatcaatta
     1561 ttatggatta agtactagtt tagctgccgc tgttgggcca tttttaggaa tgattttgct
     1621 gaatatgact gattttcgtt tcattatctg gttttcaatt gtactggtct tctttgttac
     1681 gattgcctgc cttgtctttc ctgtaaaaaa tattccatta tctgaagaac aaaaaatatc
     1741 tcttcgctca tggaatatca atagttttat tgaaaagaaa gcacttttca ttacaggaat
     1801 tgcctttttg atgggtcttt cttattcaag tgttttatca tttttatctt cttatacaaa
     1861 agtaatccac ttagttgctg ctgggtcatt tttcttcgtc gtatatgctt tggtcatcac
     1921 atttacacgt ccattgactg gaaggatttt cgatgtcaaa ggagaacaat acgtgatgta
     1981 tcctagctat ttattcttaa cagcaggatt atttttactg agtgtaacga cgaatagtgt
     2041 gacattgctt atctctggag cattagtcgg cctaggttat ggtacgttta tgtcaaatgg
     2101 acaggctgtt tgtttaaaaa tcgtccgcga acatcgaatc agtattgctt tatctaccta
     2161 ctttattggt ttagacctag gcttaggcgt tggtccttat ttgatgggga tgctaaaatc
     2221 tcttacttca tttagaggat tgtatgtgat tgccggagtc attccattga tatgtacagc
     2281 actctatctt gttaatggcc gaaaacatag tcatgaagca gagggcgaat tgattgaatc
     2341 attgaaaaat gaaatttgat cttgatgaat agataaaaaa aaccgttcag atctgaaaat
     2401 aatctgagcg gttttttgag taaaaatagt ttatgattat tcttaattaa acatctattt
     2461 atttttaaag ctttcattta tcgataagaa tagtttaact gttgatttat tccattttcc
     2521 gttcatctgg tacgatgcct tgacttagca accacttgtg gaagaaccat tctgccacac
     2581 cgatcaatag agcaggaatc aatactttca tccaaatatc gactgtatca ttcataaaat
     2641 atagatagag ccacaagata actgcagctg aaccagcatc tgcgatcgtc gctgcgatgt
     2701 tccctacttt acgtaagatc aacagatccc ctagcacata gatcatgact acaataatta
     2761 ccccaacaat cgttgaatcc ataaagctaa caccgtataa acctgtcagc acgacccaca
     2821 gaatcgccca gatcatgatt gccttcacta ccaacgcctt cacatgtttc attttctgca
     2881 ctcctttctc ctggataaaa aagtaagatt atctaacaag ataacctttt cattattata
     2941 ccatcggctg agagaagcga gcaaagataa ggtcctatct aaaaatgagg aagccactta
     3001 gaacattcac gtaataattt atccgtacag acattcgata tggtgttcct ttactttcgc
     3061 atactgttct tttgtcagtt ctttacttgt caccagataa tcgatttcat gaaaagcaat
     3121 ggcttgatgc atgaatttat gtgcaatttt ttgctgtgta actaaaacgg caagccggtc
     3181 tgattgtttt ctaaaagctt ttttgaccat tgcctctcct tcatgagttt ctgttactcc
     3241 ttggctgaaa tctagcccgc gacaactcat tatcgctaaa tccgcataat atgacaatgc
     3301 atcgttatag gcttttgaac cgttgattgt tgctcgtttc ggaacaattg cccctcccaa
     3361 tacagatact tttgccgttg tatattcact caacatagca gctgtgacga tgccattcgt
     3421 aatgatctgc aaaccatcaa atcttgctag gtttctgact aagtgtaaag aggttgtact
     3481 ggaatccaag aataagacca tatcatctcg aatcaaagaa gaagctgtat ctgcaatctt
     3541 acgtttttct gtttcttctt ctaatttggt gatcttcatc aggtaaggca cgtcaatttt
     3601 tcgattgtca ataagcatgg cgccaccgcg aactctacgg atcaatccac ttttttccag
     3661 aaaaacaagg tcacgtttaa tcgtggcttc gctataattt actaactcga tcaaatcggt
     3721 gactcttgca aattggttgt tttttagata attcacaatc tcctgctgtc tattctccac
     3781 ttcattcaac ataaaatcac cccatttact ttatatagta acaggaaaaa agaggttgtg
     3841 acagaagtga ttggcttcga gaaataagaa gaaatttttc gaaaaaacta cttctgaaca
     3901 tcgtgtgtgg ttaagagggt atgacaagac tattgtcata ccctcttgac cacacatttt
     3961 tatttttagt ataaactagc cttgcctact gtgttcaaaa tttccatttt atgtttgacc
     4021 acttcttgta cctttttatt agctgctgga taaattgcat tcggctcgta catcgatgga
     4081 ttttcactca atatttctct caaagcattg aaaaagacgc ttttaagatc tgagctcaaa
     4141 ttcactttgg caactccgta ttttactgct tcgctgactt ctgcgtccgg attacctgaa
     4201 ccgccatgta aaacaaatgg aagatcgatt tgttggttca attctttcag taagtcgata
     4261 tttaattttg gagttctgtt tttcggatac aagccatgcg ctgttccaat tgctacagca
     4321 agtgtatcga ttcctgtttc tttcacaaaa atcttcgctt gatcaggatc tgtataaatg
     4381 attgtttcag cacctccttc agcagaacca ttattcccaa tcgttccaag ctcagcctcc
     4441 actgagacat ttaccgcatg agctaacgca accacttttt tcgtaatctg tacattgtcg
     4501 tcgaatgatt tcaaagacgc atcgatcatc acggaagaat agccgtttcg aatcgcccgt
     4561 aagacatctt ctatcgtacc gccatgatcc aagtggataa cgatcggcag tttacttgta
     4621 taagcatagg ctcttactgc cgcaacaaac tcatcactta aataggcgat ttcatcagga
     4681 tgaatttcta agattactgg tgcttctaat ctctctgctt cttcgatgat tgctttcatc
     4741 atatcatagc tgcaaatatt gaaggctggt actgcaaatt gattctcttt agccactttc
     4801 aataactctt tcattgtata taacattacg cttccacctc atttgttaga ttttcatttt
     4861 tcgtccactt gtcttgtttt ttctctttca ctgttttttt caatagtcca agcataaagg
     4921 ctgtagtgac tgtacctgat gcaattgcca agacatacat aggtaaattg ttcataacat
     4981 ttggaattag catcacccaa atccctccat gaggtgccat tgaagtaact cctactgcca
     5041 tggaaatccc tgcagcgact gctgaacctg tcatcaaact aggtatcacg cggatcgggt
     5101 cagataccgc aaacggtata gcaccttctg taatatacga agcgcctaaa atccagcatg
     5161 attgacctga agtccgttct tctttggtaa atttatgttt gaataataaa gtggctaaag
     5221 caatacctag gggaggtgtc attcctgcag ccatacaagc agcaatcggt gcatttactc
     5281 cagctgacat caaaccgatc gagaaagtcg aagtcgattt attgatcggc ccacccatat
     5341 cagctgccat catgatcccg ataagtaatc cgaacaatag cccggatgta tttcccaagc
     5401 tattcagcca atttgtcaaa gcatccaaca cccatgtgat cggttgttca atcacaaata
     5461 ccatcaaaag cccaataatt aagatcgata aaaaggggac gacaatcagc tgataaatag
     5521 cagaaaattc tttttttacg ctaattttat ttgccagaaa atcacagata taccctgcta
     5581 gcacgccacc aatcattccg ccaagaaaac ctgaaccacc tgtagaggcc agtaatccgg
     5641 caacaattcc tggggcaaaa cctgcttttc cagcaatcga tgtagcaatc cccgcgccta
     5701 acgcaggaac catcatggca aacgctgtat ctcctccgat ccttgaaaat gcagctgcta
     5761 acacattata gtctgctgag ttgggatcag ccgctgtaat cccaaaggca aaactcagtg
     5821 cgatgatgat tccgcctgcg atcacaaatg gaagcatgta attcacacca ttcatgaaat
     5881 gattatagat tgtcggcttt ttctcatttg cagctggttc tttttcatta gacacaacga
     5941 atacgccaat atctctcaat gcttcgtcta ttactacttg cggttctttg attgctcggc
     6001 ttgtagagac acgctttact tttttcccgg caaatcggtc caattcgatt tctttatcta
     6061 ctgcaagaat gattgcatct gcctctttga tttcttgttc agataactga ttttctgttt
     6121 ttacaccttg tgtttcgaat tttgcttgat ggtttagatt tcttgctgtt acttctaatt
     6181 tttctgccga catataagta tgagcaattc cagtcgggca agcagttaca ccaacgattt
     6241 tcatgattat tttcctcctt tttcttcact atcgatactc ggacaaagac tctactattt
     6301 tcattcttga ctaatctgtc tgatgatatc tgctggcgta gtggcttctt ttattccctt
     6361 tctaaaatct gcattcatca atcgtctgga gagtgtgctg agaatctcta gatgcatatc
     6421 tgctgatttt tcaggaacta cgatggcaaa aatatattca attggcattt catctatccc
     6481 gctatagtcc gttaatttgg ctgtatgaaa aaaacacagt gctggttttg tcacatattt
     6541 gctttttgca tggggaatcc caaatccatc ttctattcct gtggagatct cctcctctcg
     6601 tttgaatagt gccttaacga ctttttgaac atttcttacc ttatttgttt ttttcaacac
     6661 atcccctatt cgctcaaata atgatccttt tgaatctatc gatggatcga aaacaatcag
     6721 ttcttctgcg atcaattgat caaacctcat aaaaaacctc tcccccacaa aaaatgttaa
     6781 cgttttcttg gaactgaggt cagtatagca tcgctagctg taccgttcaa tattatgaat
     6841 gaacagagga aaaagaccca tcatccgcgt gtataagtca ttaaaatgaa cgatctagct
     6901 cacaaacaat cgcaagtcta taacaaaaca aaaagccttc cttgaaagga aagcttagct
     6961 tctaatttta ggtaaatggt tttttacttc gaaataaccc ctcttcattt cgttaatata
     7021 aggtgttttt atcaagtgga taattaaaac tatatactag tttttcattg ttttgtgcat
     7081 tatgatccta cgactttttc agcatcaaac gaagttttaa attggcaaaa aacataaaaa
     7141 aacagactat ccctatcaga tagtctgcta aacgttgagt tatcagcgtt tttattattc
     7201 cgcagcgttt gcgtctttga gaccgtattt tttgttgaaa cggtccacac gtccgtctgc
     7261 ttgtgtgaat ttttgacgtc cagtgtagaa tggatgtgag tcagaagtta cttcgacacg
     7321 gatgactggg tatgtattac cgtcttccca ttcaactgtt tcttgtgaag tttttgttga
     7381 acctgacaag aatttgaaac cagttgttga atccatgaat actactgggt ggtaatctgg
     7441 atggatgttt tctttcatga ttttttcgct cctttgccct gctccatttg ctgaaccaga
     7501 gttgatttgt cgatttacaa cattaacatc ttaacatgtt tcagtcaagg ttgcaattat
     7561 cttttcagat ttctggtcgg atttttttta ccaaaggaga catcttgaaa ttctttgaaa
     7621 aattgctgat tgttttttgt tttacgcaaa aagcggatca gttgttccgt atattctaat
     7681 gaatctccag tcatattttt tcgaagtttc caaatttctt ctaattcttc acttggcatc
     7741 aataattctt cttttcgggt acctgatttt ttgatatcga tggctgggaa gatccgacgt
     7801 tcagataatt cacgtgaaag ttgtaattct agatttccag ttcctttgaa ttcttcatag
     7861 atcacgtcat ccatacggct tcctgtatcg actaaagcgg tcgctaaaat cgtcaaactg
     7921 ccaccttctt cgatgtttct tgctgccccg aaaaatttct taggtttata caatgcggct
     7981 gggtcaatcc cgccactcaa tgtacgtccg cttggtggaa cgactaagtt ataggcccga
     8041 gcaagtctag taatactatc catcaagata acgacatctc gtttatcttc gactaaacgc
     8101 attgcgcgtt ccaatactaa ttcagaaact cgagtatggt tttgcggctg cagatcaaat
     8161 gtagaagata ccacgtcgcc tttcacgctt cgttctagat ccgttacttc ttccggtcgt
     8221 tcatcaatca gtagaacgat taattctact tccggatagt tttcggtgat accatttgcg
     8281 atttctttta atacagaagt tttccctgct tttggcggtg cgacgatcaa tccacgctga
     8341 ccaaaaccga ttggagaaaa gatatcgatc attcgggtag atagtctttg cggtgttgtt
     8401 tccagttgaa gctgtctttg cggataaaga ggtgttaagg ctggaaaatg cggacgttct
     8461 tttgcttctt ccggatcttt tccattaacg ctttccacgt gcatcaaacc ataataacgt
     8521 tccgattctt taggtggacg ggcttttcct gccactttgt ccccatttct caaactgaat
     8581 cgtctgattt gtgaggcaga aatatagata tcttctgcac ttggtccata attgattggc
     8641 cgtaaaaaac cgtaaccatc ttgaccaacg atatccaata ttccttccat ataataaaat
     8701 ccttgtttct ctgcttgtgc acgaatcaca gctagagata attccttttt attcatctta
     8761 ctgtaataag gaattttaaa ttctttggca taactataaa cttcttttaa cgttttgttc
     8821 tctagttctg ccattgtcaa ataatcgctc attcagctcc cacttcctct atcatttcaa
     8881 ttgatgcccc taaggctgtt agtttctcga taatatgatc ataaccgcgt aagatataat
     8941 ctacattgta aatcgtcgtt gtcccttctg ccaatagtcc agcaatcact aaacaagcac
     9001 ctgcccgaag atcagaagcc acgacttctg tgccgtgaag tttatttgga ccattcacga
     9061 tgatcatatt tccttccacg gtaatatcag cgcccattcg tgctagttcc ggcacatggt
     9121 tcacacgttt cgcatagatc gtatcgatga tttctgaagt gcctgtagtc atcaatagta
     9181 acgcagtaag tggctgctgc agatctgtgg caaatcctgg ataaggatag gtcatgatgt
     9241 ttgcgggttt taattctttc gaggggtaaa cttcgatttc atcttcgctg attttcatat
     9301 ggacaccaat ttcctgcaat tttgctataa agctttccaa atgttcaaag atcacatttt
     9361 tcactttgat accatttcct actgcagctg ctaatgctag ataggttcct gcttcgatgc
     9421 ggtcaggaat catgaaatgc cgacagccat gtaatgtttc gactccttcg atacggatca
     9481 catctgtacc cgctcctcga acttttgccc ccatattatt cagtaaagtt gccacatcta
     9541 tgatttccgg ttctcgtgca gcgttttcaa ttactgtctg tccttttgct tttacagcag
     9601 ctaacatcac attgatcgtt gctccgactg aaaccacgtc catgaaaatc cgattgccac
     9661 gcaatccttt attttctgtc cgtaaataca tggcaccatg ttcatttgtc acttcagcac
     9721 ctagagcttc aaatcctttg acgtgtaggt caatcggtct agggcctaaa taacagccac
     9781 ctggcaggcc tactaccgct tcgccaaatt taccaagcag tgttcccata aaataataag
     9841 aagcacgcaa actattgatt tttccgtgcg gcatcggaat cgatacgact tctcgaggat
     9901 cgatttctag tatgttgttg gaaaaatgga cagttgcgcc catgatctct aaaatttcaa
     9961 ttaatgaatg aacatcttgt atgtccggca caccttccaa gatcactggg gaatcagcta
    10021 agattgcagc tggaatcaat gcgactgcac tattttttgc accgctgatc gttacttcac
    10081 cagctaaagg tcggtttcct ttgattacga tttttttcat ctgtttccac cttgtttatt
    10141 aatctttttt catggatatg cttgtatttt atcatagata aaaaaaagaa acgagcatgg
    10201 tctgccgttt taagtaaaaa agaatggaga gcgacttcaa aagtcgttct ccattcctat
    10261 atatcgtatc tgttatgtgg atgcttacgc tttgttagct gaaccgaacc agtcgatatg
    10321 agatttagca tcttcaacga ttgctgcttt acctggagct aataatttac gagggtcgaa
    10381 tcctttacct tctttatctt taccagcttc gatatattta cgagtagctg cagcaaatga
    10441 taattggaat tcagtgttaa catttacttt tgagataccc attgagatag ctttttcgat
    10501 ttgatcttga ggaatacctg atccgccatg taatactaaa ggcatgtctg gacctacagc
    10561 atcagcaatt gcttgtaagt ggtcaaaagc taatcctttc cagttttctg gatattgacc
    10621 atggatgtta ccgataccgc aagctaagta gtcgatacct gtagcaacca tttgtttgca
    10681 ttcttcaata tcagctaatt cgccgttacc gatgattccg tcttcttctc cgccgattga
    10741 accaacttca cattcaactg agataccttt tgcatgtgct ttttcaacaa cgtctttagc
    10801 taatttcaag ttttcttcaa atggtagatg tgaaccatcg aacattactg aagtatagcc
    10861 taattcgata cattccaaag cagcttcgta atcaccatgg tctaagtgga tagcaactgg
    10921 aacagtgatg ttcattgctt caactaaatc agtgattaaa tctttacaga ttttgtaacc
    10981 acccatgtat ttagctgcac ccattgatgt ttggataagt actggtgctt gttttgcttc
    11041 agccgcttca aggattgctt gagtccattc caagttgttt gtgttgaatc cgccgacagc
    11101 gtaaccgcct ttacgagcag cttttaaaaa ttcagctcct gatactactg gcataataaa
    11161 attcctccta agttttgtaa aactaaattt gttaagacaa ttcttaacca acgaccctat
    11221 tttaccaaac atttcggtag ttttctacta aaatcaatga aaatgaaaat tttcaagaag
    11281 ttttttgcaa aaaataaaat atccaatagc gctttaatcg aaaaaagtct tttgctttct
    11341 atcttttttc ctacaaataa taacaaatca gaaaaagaga aaagtgacaa gaaaattgct
    11401 tatccccttc tcttgctact tttctctttc tatcaagttc aaaaattatt ttgtttcgtg
    11461 atctgctaat gatgctccga caaatgcttt gattaatttt tgcgggcgat ttggacgaga
    11521 aatcaattct gggtggaatt ggcatcccac aaagaatttt ttctctggca gctctacgat
    11581 ttctactaaa cggttatctg gtgagacacc tgaaaagact agcccttgtt cttcaaacaa
    11641 ttgacgatat ttgttattga actcatagcg gtgacggtga cgttcttgca caacatcggc
    11701 tccatcatat gcttcagccg tcttcgttcc ttttttgatt ttacatgggt ataatccaag
    11761 gcgtaaagtt ccacccaaat tctcgatgtt ttcctgatca gccattaaat caataatatt
    11821 gtttgctgta tctggattcg tttcagctga tgacgcatct tctaatccaa cgacgttacg
    11881 tgcaaattcg acacaagcca tctgcatacc aagacaaata cctaaaaacg gaacgtcatt
    11941 ttcgcgagca taacgaatcg cttcgatttt cccttctact ccgcgatcac caaatccacc
    12001 aggaaccaag attccatctg cgtctttcaa acgttcttct acattttctc tcgttaattc
    12061 ttgtgctttg atccaatcga tctcgatgtc tgaattatag gcgaaaccag aatgtttcaa
    12121 tgcttcaact actgagatat atgcatctgg taactcgaca tatttaccga caagcgcaat
    12181 ccgtactttt ttcttcaagt tcaagacacg ttcttctaat tctttccatt ctgtcatgtc
    12241 tgcttctggt gtatctagtt ttaaatggtc gcaaacaatt tgatccattc cttgtgcttg
    12301 taaaactaat gggatggagt aaagtgtatc tacatctggc gactcgataa ctgcttctgg
    12361 ttcgacatca caaaattgtg ccaatttgtt tttcacgctt tgagaaacag gttgctctgt
    12421 acgtaccacc aaaatattcg gttggatacc taaaccacgt agctctttta cactgtgctg
    12481 cgttggttta gttttcattt ctccagcagc tttcaagtaa ggaatcaatg ttgtatggat
    12541 atacataaca ttatctgtac cgacatctgc tttcatttga cgaagtgctt caaggaatgg
    12601 caaagattct atatccccta ctgttccccc tacttctgta atgatcacat ctgaatcagt
    12661 cattttagct gctcgcatga ttttttcttt gatctcattt gtaatatgag gaataacttg
    12721 aacagttgct cccaagtatt cgcccttacg ttctttacgc aaaacttcag aatagatttt
    12781 tcctgtcgtc acgttggagt atttgttcaa attgatgtca ataaaacgtt cgtagtggcc
    12841 cagatccaaa tctgtttcag caccatcatc tgttacgaat acttctccgt gctgatatgg
    12901 gctcatcgtc cccggatcca cgttgatgta tggatcaaat ttttggatag ttacttttaa
    12961 gccgcggttt ttcaataagc gtcctaaaga agctgcaaca atcccttttc cgattgatga
    13021 aacgacaccg ccggtcacaa aaatatattt tgtcataagt tcaaaaactc ctttttaatt
    13081 tatgtgcagg aaaagtctca gacaataaac aaagctccct attcttcaat aagaataggg
    13141 agccgaaatt tctgtaaact tctgaccctc taaaagggtg cccaaggtgt ataatacaag
    13201 ctgactaagg gaaagtcaag tactcaaatg aatactatcc gcttacaatt ttttcttttt
    13261 cgatatcctt tgagaaatta tttcttaagt ccttta
//
LOCUS       DACEOE010000064        13280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEOE010000064 DACEOE010000000
VERSION     DACEOE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.066"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            214..828
                     /gene="gmk"
                     /locus_tag="IW774_001535"
     CDS             214..828
                     /gene="gmk"
                     /locus_tag="IW774_001535"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1153885.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            833..1138
                     /locus_tag="IW774_001536"
     CDS             833..1138
                     /locus_tag="IW774_001536"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1153886.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1391..3799
                     /gene="priA"
                     /locus_tag="IW774_001537"
     CDS             1391..3799
                     /gene="priA"
                     /locus_tag="IW774_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1153887.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3814..4305
                     /gene="def"
                     /locus_tag="IW774_001538"
     CDS             3814..4305
                     /gene="def"
                     /locus_tag="IW774_001538"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1153888.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4298..5236
                     /locus_tag="IW774_001539"
     CDS             4298..5236
                     /locus_tag="IW774_001539"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1153889.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5236..6594
                     /gene="rsmB"
                     /locus_tag="IW774_001540"
     CDS             5236..6594
                     /gene="rsmB"
                     /locus_tag="IW774_001540"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1153890.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6607..7347
                     /locus_tag="IW774_001541"
     CDS             6607..7347
                     /locus_tag="IW774_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1153891.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7344..9413
                     /gene="pknB"
                     /locus_tag="IW774_001542"
     CDS             7344..9413
                     /gene="pknB"
                     /locus_tag="IW774_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1153892.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9578..10477
                     /gene="rsgA"
                     /locus_tag="IW774_001543"
     CDS             9578..10477
                     /gene="rsgA"
                     /locus_tag="IW774_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1153893.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10490..11140
                     /locus_tag="IW774_001544"
     CDS             10490..11140
                     /locus_tag="IW774_001544"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1153894.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11141..11779
                     /locus_tag="IW774_001545"
     CDS             11141..11779
                     /locus_tag="IW774_001545"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1153895.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            11934..12788
                     /gene="mreC"
                     /locus_tag="IW774_001546"
     CDS             11934..12788
                     /gene="mreC"
                     /locus_tag="IW774_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1153896.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12792..>13280
                     /gene="mreD"
                     /locus_tag="IW774_001547"
     CDS             12792..>13280
                     /gene="mreD"
                     /locus_tag="IW774_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1153897.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFK"
BASE COUNT         4387 a         2451 c         2947 g         3495 t
ORIGIN      
        1 gcgataataa ccccaaccaa acgaaaaaca tgagaagctg tttatgtttg gctgttctta
       61 ttacttgatg caggacagct tttttacgac ctcttggtac agggtagctt ttttaccaat
      121 ttctagctat aattaaagat aaccttgaaa gttgcagaaa aaaagtgcaa aataaacgat
      181 aagtacaaaa agactagttg aaaggacgtc gtcatgtcag agcgtggatt attaatcgtg
      241 ttgtctggtc catccggtgt aggaaaaggc acagtacgaa aagcaatttt tgagagtgag
      301 gataatgatt ttcagtattc gatctcaatg actacccgta aaatgcgtga aggggaggtc
      361 gaaggcgttg attatttctt ccgaagcaaa gaagagtttg aatcaatgat cgaagctgga
      421 gagatgctgg aatacgcaga atatgtcggg aattattatg gaactccttt gtcatatgtc
      481 aacaagacat tggatgaagg aaaagatgtt ttcttggaga ttgaagtcca aggagcaaaa
      541 caagtcaaag aaaaagtgcc cgatggtgta ttcatctttt tgactccgcc agatttagcg
      601 gaattaaaat cacggatcgt aggccgagga acagatgcag atgaagtaat cgaagaacga
      661 atgaaagttg caaaagaaga aatcgaaatg atggctcttt acgactatgc tgttgtaaat
      721 gatgaagtgc cgctagcagt tgaacgaatc aaaaacatta ttgccagtga acattttcga
      781 gtagatcgcg taatcggtaa atatattaaa atgttaaagg agatgtagtc ttatgatgtt
      841 aaaaccttca attgactcac tattagatcg tgtaaattca aaatattctc tagtgatttt
      901 agcaagtaaa cgcgcgcatg aattggatgc aagtgcacag cctactctag attcttttga
      961 ttcagtaaaa agcgtcggtc aagctttaga agaaatcgat gcaggaaatg tagtcaatga
     1021 tccacatcct gaattaaaac gtgaacgttt gaagatggaa gaagaggaac gtcaagcaca
     1081 aaaagagcgt gaacaacacg aattggaatc acgtatccgt gaagagcaaa aaatgtaata
     1141 aagaaaaata gagcgaacat ctaaaaagaa aagcaaaccc agctgtttcc ttttcaaaca
     1201 ttcgccactt attgtctggt agaaaaggct gtgtctgaac tttttgacac agttcttttt
     1261 tttcttatct gctgttaatt ttttgttagg atttggcaga agataagaca aatccatcga
     1321 tctctagtaa aataggcaag gaactttttg tcgcttgata agaaaagaca aaaaaaggag
     1381 gaaacgaacc atgacattaa cagctgaagt cattgtggac gtacctacta tgcagaccga
     1441 tcagccattc acttatttgg ttccctctga aatggaaaca gctttgcaag tcgggatgcg
     1501 agtagaagtc ccttttggta atggcaatcg tcatgtccaa ggatttgtta tggctatcca
     1561 aaaatcagaa gaatcagcta atccatcttt gaaggcagtt attcgtttgc tagaccttgc
     1621 tccggtggtc aatgaagaat tactttcatt agctgattat atgaaaaaaa ttacttacgc
     1681 attcaaaatc acttgtctac aaacgatgct tccgagtgtg atgaaagcag aatatgataa
     1741 attgatctat cctttggcag acacaccaga agttcaggta ttgtttgatc agcgagaagt
     1801 gatttcctgg aaggaagcag aagaagaagg cagcctttct caattgattc gctggcgcca
     1861 agagcaatta gtagatatca aatatgaagt ccatacacga aataaagtaa aaacgattcg
     1921 tttggtccgt tctttgttaa ctgaaaagca aatcgaagaa gaatgggcaa aattacggca
     1981 aaatgccaag aaacaaaaag aacttctttt gtgtttatct gaactgagcc aagaagaacc
     2041 aattgcttat ttcaaaaata aaggaatcag tactgctgtc ttgaatcaag gaaaagaaaa
     2101 gggctggctg gagtttatag aatcagaacg ttatcgcgac ccttataaag accgagtatt
     2161 tgaccaaacg acggcattgg aactgaatgc agaacaaaaa aatgctgtcg aacaaatcat
     2221 cacagcaggt cagcagcaaa aagatcaggt ctttttgctg gagggaatca ctggcagcgg
     2281 gaaaacagaa gtatatcttc aggcaatcgc tgatgttttg tcagaaaata aaacggctat
     2341 tatgttagtg cctgaaatcg cattgactcc gcaaatggtc gagcgattca aaggacgctt
     2401 cggagaatca gttgcggtct tacatagcgg actttcccaa ggtgaaaaat atgatgaatg
     2461 gcgcaagatt gagcgggaag aagcgcaagt agtagtaggc gcacgatctg ctatttttgc
     2521 cccgctgaaa aatatcgggc tgatcattat cgatgaagaa catgaatcaa gttacaaaca
     2581 agatgagaca ccgcgttatc atgcgagaga tctagcaatt tggcgcagta aatatcatca
     2641 ttgcccaata gttctaggaa gcgcgacgcc ctctttagaa tcaagagcac gagctcaaaa
     2701 aggcgtttat cagttattgc agttaaacca ccgagccaaa gcagctgcac agcttccagc
     2761 tattgagatc gtagatatgc gtgaagagtt tcagaatcat cggacctcta ctttttctgc
     2821 taatttgcag gaaaaaatac aaaatcgatt agacaaaaaa gaacaaactg tcctactgct
     2881 caatcgtcga ggatattcgt cttttgtgat gtgtagggac tgtggatttg ttcttccatg
     2941 tcctaattgc gatatctctc ttacactgca tatggataca cgttcaatgc gctgccatta
     3001 ttgcggacat gaagaaccta ttcccaatcg ctgtcctaat tgtggcggca ataaaatccg
     3061 ctactatgga acaggtactc aaaaagtaga agaggaactc cgagaacttt atccgcaagc
     3121 taggattttg cgtatggatg tagataccac tcgaagaaaa ggggcacacg aacagatttt
     3181 acaaaaattc ggtgcaaaag aagcagatat attattaggg acacaaatga ttgctaaagg
     3241 tttggacttt ccagaagtga cattagttgg ggtgttgaat gctgatacct ccctgaattt
     3301 accggatttt cgttctagtg agcatacatt ccaattgctt acccaagttt caggacgagc
     3361 agggcgagcg gaaaaagcag gcgaagtcgt gatccaaaca ttcaatcctc aacattacgc
     3421 catagaattg gctaaaaaac aaaattatga gcagttttat cagcaggaaa tgcatgtacg
     3481 gcatcgtgga ggctatcctc cttattactt tactgtgaaa ataaccgcca gccatcctga
     3541 agaacaagta gcggcaaaga aaatctttca gattgccaat cagctaaaag aagtgttaag
     3601 ccctcaaagc ttgctattag ggccaacgcc cagcatgatt ttgagagtga aaaaccgata
     3661 ttattatcaa ctaatcatca aatataaaca tgaaccgaat cttcctcatg ttttggatga
     3721 aattttgaat ggaagccaga aagaacagcg acaaggtctt tttgtggcaa ttgacaacga
     3781 accattaaac tttatttaag aaagggccaa attatgcgct atcctatatt gatccaccct
     3841 aatgataaat taaaaagaac tgctcagcca atcgatgtaa tcacggacga gacaatcgct
     3901 ttattagaca atttatatga gacaatgatt gcgaatgacg gaatagggat cgctgctcct
     3961 caagtaggac aaaacaagag aattgctgtg atcgaagtag atgaaggcga gaaatttgaa
     4021 ctgattaacc cagaaatcat cgaagcaaaa ggtgaaagcc ttgacgtaga aggttgtctc
     4081 agtattccgc atgtctatgg aacggtcaag cgagcagatg aagtgaccgt gcgttattat
     4141 gatcgagacg gagaagagat agaagtgacg gcttttggct atttagcccg tgccttccag
     4201 catgaaatcg atcacttaga cggtatcttg ttcattgaga aaatgataca gcagatcccg
     4261 gaagaagaac ttgaagaata tatggaggaa catcttgatg actaagctag tatttatggg
     4321 cacacctgct ttttctgtgc cgattcttga agggctgata gaatcaggtt acgagatcca
     4381 ggcagtggta acacagcccg accgtccagt tgggcggaaa cgagtaatta cgccgacacc
     4441 ggtaaaagaa gcggctctga agcacggtat ccgtgtgctg cagccagaga aaatctccgg
     4501 ttcgccagaa atggaagaaa tcattgaatt ggtgcctgat gtgatcgtca ctgcggcatt
     4561 tggccaattc ttgcctgaaa agttattgca agtaccaaaa ttaggggcaa tcaatgtcca
     4621 tgcttcttta cttcctaaat atcggggagg tgcgcctgtt cattatgcca tcatgaatgg
     4681 ggagaaagaa actggtgtaa cgatcatgga aatgatcaaa aaaatggatg caggcggaat
     4741 ctatgcgcaa gaaagcatac caatcactaa acaagatgat gtagggacga tgtttgaaaa
     4801 gcttagcctt ttaggaagaa agctattgct agaaacattg cccaatattt tggatggaca
     4861 aaaaccagta cctcaagacg agtcagaagc aactttttcg ccaaacatca cacgggaaca
     4921 agaagcaatc gattggcata aaactgcaga agaaattgac aaccaagtac gcggaatgcg
     4981 tccttggcca attgcattca cgacctatga gggcatacgc tggaaattat tatcggttga
     5041 agtattggca gaaactacag acaaagcacc tggtacgatc atccggaaag acaaaaaaaa
     5101 tctatggatt gcttgcggta aacagaccgt cttagccatc aatgaattac agccagctgg
     5161 taaaggaaaa caggccgtac atgatttcct taacggaagc ggtcagcaag ttcagattgg
     5221 tcagcaggtg aaataatgaa aaaaatccct aaaaaaatga aaaaatcagt acgatatatg
     5281 gcattactga cattagagcg agtgaacaag ggtggcgcct actcgaatct tttgttgaat
     5341 gagatgatca aacaaagcga gctaaatcaa aaagatgtgg gtttatttac agaattgatc
     5401 tatggtacga ttagccatca gcgactattg gaattttacg ttgcaccatt gatcgctaaa
     5461 gcaaaaaaag tggatgactg ggtcaagacg cttctttatc tttctgtata ccaattagaa
     5521 tttttagata aagtccctgc acatgctatt ttaaacgagg cggtcgaaat cgcaaaagta
     5581 aaaggaaatc ctgggacagg gaaatttgta aatggtgtct tgaggaatta tcagcgtcaa
     5641 ggagcaccag atctgcaagg cattgctgat cctatcgaaa gactttctgt caaaatcagc
     5701 ctgcctctat ggatgactaa acgactggtg gatcaaatcg gatacgaaga aactgaaaag
     5761 ctcggtttat cgctatatca gcctagccat gctagtgccc gagtagatac acgccgactt
     5821 tccagagatc aggccatcac tgttttgcaa gaagaagaga tcacagctga aaaaagccaa
     5881 gtcagtccat acggtatagt agcgaaaaaa gggcatttag cttctagctc attgttccac
     5941 gatggcgtca tgacgatcca agatgaaagt tcgatgcttg tagcgccgtc tatgcaaatc
     6001 gagcctgaac atcatgtctt agatgcctgt gcagcacccg gaggtaagac gacgcatatt
     6061 gcttcttttt tagatccgtc atgcggtgga gaagtggtcg ctttagatgt acacgaacac
     6121 aaagtcaaac tcattgaaga taatgcagca cgtctaggtg tctcttctgc tgtctacgct
     6181 caaaagatgg atgctagaga agtcagtaaa cagttcgaag cagagcagtt tgatcggatt
     6241 ttagtagacg cgccttgttc aggtcttggg ctgcttcggc gaaaaccaga tattcgttat
     6301 aaaaaacagc cggatgaatt agcgaattta ccaaaaatcc aattggaaat tttagaaagc
     6361 tgcgcaccaa ctttaaagtc ttccggtatt ctgacttata gcacttgtac gatcttaaaa
     6421 gaagaaaacc aagacgttgt ttctgctttc ttggaaagac accctgattt tgagaggatt
     6481 gatgttttga ccaatcctat gctacaatca tcaatcgaag agaaaatgtt gactttgtat
     6541 ccgcatcagt tttacacaga tggatttttc atttgttgtt tgcgcaaaaa gtaatgaggt
     6601 gaaaaaatgc agattgaata tcaatcagat gtaggaagac gacgtaacac aaatcaagac
     6661 tatgccagtg tatttaccag ccgttcaggt ctcaagcttg ctgtattagc agacgggatg
     6721 ggtggtcatc gtgccggaga cattgccagt cagatggcgg taacgaatct aggcgaagct
     6781 tgggaaaaac aagaagctgt tgacgatgaa aaaatcgccc aatggttcat tcaatcgatt
     6841 caagaagaaa atatgctgat ttatcagcgt ggacaagaac agccggaata taacggtatg
     6901 ggaacaacaa tcgtagcggc cgctctgtca gatgactggt tcacgattgc caatgttggg
     6961 gatagcagag cttatttgat tcgtgaagat aaaatcatgc aattgacgga ggatcattct
     7021 ttagtcaatg aactagtcaa atcaggagaa atcagcgaag aaatggcagt gaaccatcca
     7081 aggaaaaata ttttaacacg ttctgtcggc atgcctggaa cagtcgaagt agatgtttct
     7141 actcatcttt ggcaactaaa agatcgactg ctgttatgtt cagatggctt gacgaacatg
     7201 ctgtctgaag aagaaatagg aacgatcatc aatcaagaag gcccattgtc tcataaagta
     7261 actgagctga ttgatcgtgc caatgaagca ggcggagcgg ataatatcac cgttcttctt
     7321 atcgaatata aggaggacag tgtatgatcg agttgggcaa aaaattaaat ggccgttatc
     7381 aaatcactgg aaatatcggt agcggtggta tggcaaatgt atttttggcg cacgatttaa
     7441 tcttggatcg tgatgtagca gttaaagtct tacgatttga ctttcaaaat gaccagactg
     7501 cgattcgacg atttcagaga gaagctttag cagcaacaga actcgttcat ccaaacattg
     7561 ttagtgttta cgatgttggt gaagaagaca acatgcagta tctggtgatg gaatacgtca
     7621 aaggaatgga cttgaaacga tatatccaaa cacattatcc tgttccttat gaaacagcag
     7681 taaatattat gcagcagatc ctttctgcta tttctttagc tcataaccat caaatcatcc
     7741 accgagattt gaaaccccaa aacgtactga tcgataatga aggtgttgta aaaatcacgg
     7801 atttcgggat tgctatcgct ttatctgaaa catcgatcac gcagacgaat acgatgctag
     7861 gatctgttca ttatctgtcc cctgagcagg ccagaggaag tatggcaacg aagcagtcag
     7921 atatttatgc tttagggatc atcctttatg agatgttgac gggtagcgta ccgtttgatg
     7981 gtgaatctgc tgtaacaatt gctttaaaac attttcaaga tgatcttcca tctataaaag
     8041 cacttgatcc aaatgtgcca caagcactgg aaaacgtgat tttacgggca acggctaaag
     8101 aacctgcaga ccgttataag tcagccgagg aaatgtctga tgatctttct acagtattat
     8161 caccagctcg tgcaaacgaa gaaaaatggc agcctcatgt gatggataat gaaacaaaag
     8221 ttatcacacc ccttacggaa gatgcaccaa tgcctgattc attcaaatct atgcctctgc
     8281 caaaagacaa gccagaaaac gaaaccttac cagaggagga agaagttctg cctgatccta
     8341 aaaagaaagg acagaaaaaa tggtggctga tcctattagc tattcttgct gttttaggaa
     8401 tcggaacagc tgtattcttt gcttctggcg ggcgaggaga agttgttgtt cctgatgttt
     8461 ctgatctttc tgaagcttct gctagagaaa cactcgcaaa agcgaaatta gaagtagcaa
     8521 caaaaacaga agaaattgca gatgacaaaa tcgaagaagg taatgtagtg aagacagacc
     8581 cggcagcggg aacgacagtc aagcaaaaac gcgaagtcac tctgtatatt agtagcggca
     8641 cgaaaaaaat caaattagat gattacagtg gtaaaagtta tgaagatgcc gaagcaagtt
     8701 tgaaagaatt agggttttct gctgacttga ttcaaaaaga agaggaattc agttcagagg
     8761 tagaaagcgg aatgatcatt tcgcaatctc catcagaaga tacagaagtc gatcctaaaa
     8821 cagacacgat tactttcaca gtcagcaaag gaccagaaac agtgactttg agtagtcatg
     8881 taggagaaaa ctacgaaact gttcgtaatt ggcttgttac acaaggattc aatatcaata
     8941 atattagtgc tacttatgac tatagcgaca ctgtaccatc aggttcgatc atcagtcagg
     9001 atccagcctc gggtgaagtt gtagcagaag aaacttacat caattttgtc gtaagccaag
     9061 gaacagagcc taaattgaat gatatctccg gctatacaaa aagcgaagca caaagttatt
     9121 tagctagtgt cggtgcagaa tatatcgggc atgagacttc cgaatattca aacacggtgg
     9181 ataaagataa agtgattcgt acagtaccag gggcaggaac gacaattacc aaaggaatgg
     9241 ttgtgaacgt aatttactct aaaggaccag atccagcgtc ttctagttct gaaaaagact
     9301 cttcagacag ttctagcagc tcaagtagct ccagtagcac aagcaactct tcaaaagaag
     9361 aatcttccag ctccacaagt tcatcagcta gcacctcaga aagttcaaaa taagtgctga
     9421 ataaaatata tcagaaggga atgcgacatt gatttgttgc attctctttt ttgtgtttct
     9481 atctcttttg atcaattttc aagccatata ctttgagaag tttgcatgga ataacaccta
     9541 ctttcctctt cgtatggtaa aataaacaaa aacggaggtg gcacttatca aaggacaaat
     9601 cagaaaagca atcagcggtt tttactatgt atacgcaaac ggagagacct atcaaacaag
     9661 aggaagagga aatttccgga atcggaaaat cacgccgctt gtcggagatc aggtgatctt
     9721 tgagagtgaa aatcaaacag acggctatct tctggaaatc ttgccgagaa aaaatcaatt
     9781 agttcgccca ccagtcacaa atgttgatct tggaatcatt gtcacaagta tcgtagagcc
     9841 tgcattttct tataatctac ttgaccgctt tctagttacc ttggaatacg agtcgatcga
     9901 accgattatt tttttgacaa agaccgactt gtcagacgac ccagaaaaaa tcgaagagat
     9961 caagcaaacc tatactaaaa taggctatcc ggtgattgta cccaatcata cgggtgatac
    10021 gacagaactg atccgttact tcccagaacg gctatcggtc tttatgggtc agtctggagc
    10081 aggaaaatcg acgctactga atcaaatatc accggaactg aatttagcaa caggagagat
    10141 ttctgatgca cttggaagag gacggcatac aacacgtcat gtggaactga ttcctatcta
    10201 tggcgggtta gtagcagata caccaggatt tagctcgatt gactttttga caattgaagc
    10261 aggagaactg ccgagacaat ttcctgaatt tgtagaagct tctgtccatt gcaaatttag
    10321 agagtgcatg catgctaaag aacctggctg cgaagtaaaa catagagtag aaacaggtgg
    10381 tatcgcacag acacgttacg acaattattt gcaattctta caagaaatcg aaaaaagaaa
    10441 accaatgtat gcaaaaaaag aaggtaaacg aaaataggag gaaacaagaa tgaagatagc
    10501 accatccatt ttgagtgcag attttgccaa tttacaaaga gacattgagt tagtggaaaa
    10561 aggaggcgca gattacatcc acgtagatgt aatggatggc caatttgtcc caaatatcac
    10621 attagggcca aatattgttc aggcaatccg tcctgttact aaattaccgt tagacgtcca
    10681 tctgatgatc gttgatccag aaaactacat tccagcattt gcaaaagctg gagcagatat
    10741 cattactgtc cacgtcgaag caactcctca tattcatcgg gcacttcaaa tgacaaaaag
    10801 cttaggagtt aaagctggag tagttatcaa tccaggaaca ccagtctcca tgatcaaaca
    10861 tgttttgcct ttggctgatc aagtactagt catgactgtc aatcctgggt tcggcggaca
    10921 aagctttatt gaagaaacag tagaaaaaat tacggaactc gctgatctac gtagagagaa
    10981 caattggact tatgcaatcg aagtagatgg cggaatcgtt ccagaaacag caaaaatctg
    11041 taaagatgca ggggcggatg tcttcgttgc aggatcatat atctatggag ccgaaaatcc
    11101 agtcggacaa atcgaacgat taaaagaggc attagactaa atgaaaattt tattagctgc
    11161 tggaggtaat ccgcaaaact ggccgcagtc tctgctgtcc aaatacgatg cctatgtcgg
    11221 gatagaccgt ggtgcgctgt acctgcttca agcaggctac ccagtagatc tggcagtggg
    11281 tgattttgat tcattgtcga acgaagagca gaaggaagta tttgcaaaag ccaaagagta
    11341 tacccgttcc cctgcagaaa aagacgatac agatacacag ttaggtatct taaaaacatt
    11401 tgaacgttac ccgcaagcgg aagtcacttt gattggagct acgggcggaa gattggatca
    11461 tcttttagcc aatctctggt taggtatgga acctcgcttc aaaccctttg cccatcagct
    11521 tattatcaga gattgtcaaa attatttgtc ttattatttg cctggagagc atatagtaag
    11581 acagatacca gatatgaagt atttagctta ttgctgcttg actcccgtct cgaatcttac
    11641 attaaaaggc agcaagtatg tattggaaaa taaagaagta gaatatccga catcttatgc
    11701 aagtaacgaa tttctttcag ataccgcaga attttctttt tcagatggta tgattgcagt
    11761 tattcaaagc aaagattgat ccataaacaa ataaaaaaat ttgtctttca ggctaaaaat
    11821 gacagaatct attatttgct aaaaaaaagc ttaagaggag caacttacct tttgtttcac
    11881 ggcatttagt gataaaattg aacacaagag tctaattaat tcgaggtgtc tttgtgaaga
    11941 agttcaatcc taataaaaat attattatta ccttggttat tgttatcatc gtggtgacga
    12001 tactaagtgt gaccgctgcg cagcgagcga atgaaggaaa gcccaatttc ttccaatcca
    12061 tcgttaatga cagtgttgca atggttgata aaacaatctc tgccccagta cggtgggtac
    12121 gaaatggagt agattctgtc cataatcttt tttcaactta tgccgaaaat gagcggctaa
    12181 aagaaaaaat cgatagctat gatgacgttt cacagcaaaa caagaattac gagagagaaa
    12241 tcgaagcgtt gaaaaaagaa cttgagttga atcagacgct gactagttac gaaaaagtaa
    12301 cggcaaatgt cattactcgt tcgcctgaca gttggcagga catgctgatc gtagacaagg
    12361 gttctaaaga cggtatcgaa gcaaatatgg cagtcatgtc gcaaaaagga ttgattggcc
    12421 gagtgatcga ggtcaatacg gcttcgtcta aaatcgaatt actgtcatcc tctaatgaaa
    12481 gctccaatca ttttccagta cgggtatctt cggctaatgg cgaagcgttt ggtttgctta
    12541 aaaactatga tgaaaagctc catgccttag tggtgaccca attaactggt gatacggata
    12601 tcaaagaagg ggatgttgtc cagacatccg gtcttggagg gaattctcca gctaacttgc
    12661 cgatcggtac ggttattaaa acgaaaccag atagttatgg gctggatcgg gaagtttatg
    12721 tgaaacctta tgcagaaatg tatgacgtgt cagttgtgac gattgtccaa cggctagcgg
    12781 aggattagtc catgttgaag aaagaaacaa tgaagtacta tctgccaatc gttttgttct
    12841 ttttgatgtt gatagatggt catttaacaa gaatgctagg ggagtggtcg aaaggcacct
    12901 atatgtcaaa tgcccacttt ctgatattgg cattattatg ttgcagtatg gcgtttgaaa
    12961 aacgttattt actgattacc acgattgttc tcggggctat ctatgacgct tactatattg
    13021 gcgttatcgg tatctatgca gtagctctcc ctttaattgt atggttgatg tatgtaatga
    13081 aagacgttat ccatgtcaac atctttactg aatttttcag tatgatcatc tttgtcacgg
    13141 gttatgaatt gtttacgatg gtggtccagt tgatttttaa attagcagta gtaaataaca
    13201 cgtattttat tacaaggttt ttaggaccta cactgctgtt gaacatgatt atatttgtat
    13261 tattcatttt tccctttaag
//
LOCUS       DACEOE010000065        13181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEOE010000065 DACEOE010000000
VERSION     DACEOE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.067"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(165..1451)
                     /locus_tag="IW774_001548"
     CDS             complement(165..1451)
                     /locus_tag="IW774_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1153898.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1444..2712)
                     /locus_tag="IW774_001549"
     CDS             complement(1444..2712)
                     /locus_tag="IW774_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1153899.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2964..3425
                     /gene="mscL"
                     /locus_tag="IW774_001550"
     CDS             2964..3425
                     /gene="mscL"
                     /locus_tag="IW774_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1153900.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3484..3930)
                     /locus_tag="IW774_001551"
     CDS             complement(3484..3930)
                     /locus_tag="IW774_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1153901.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4023..4268)
                     /locus_tag="IW774_001552"
     CDS             complement(4023..4268)
                     /locus_tag="IW774_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153902.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4382..4960)
                     /locus_tag="IW774_001553"
     CDS             complement(4382..4960)
                     /locus_tag="IW774_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1153903.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5037..5567)
                     /locus_tag="IW774_001554"
     CDS             complement(5037..5567)
                     /locus_tag="IW774_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1153904.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5571..6431)
                     /gene="rlmB"
                     /locus_tag="IW774_001555"
     CDS             complement(5571..6431)
                     /gene="rlmB"
                     /locus_tag="IW774_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1153905.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6424..6819)
                     /locus_tag="IW774_001556"
     CDS             complement(6424..6819)
                     /locus_tag="IW774_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1153906.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6816..8228)
                     /locus_tag="IW774_001557"
     CDS             complement(6816..8228)
                     /locus_tag="IW774_001557"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1153907.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8225..8761)
                     /gene="cysE"
                     /locus_tag="IW774_001558"
     CDS             complement(8225..8761)
                     /gene="cysE"
                     /locus_tag="IW774_001558"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1153908.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9083..10543)
                     /locus_tag="IW774_001559"
     CDS             complement(9083..10543)
                     /locus_tag="IW774_001559"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1153909.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10713..11870)
                     /locus_tag="IW774_001560"
     CDS             complement(10713..11870)
                     /locus_tag="IW774_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1153910.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11904..13175)
                     /gene="radA"
                     /locus_tag="IW774_001561"
     CDS             complement(11904..13175)
                     /gene="radA"
                     /locus_tag="IW774_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1153911.1"
                     /translation="MVEEIEQDTTDRRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLE
                     ELNRVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKL
                     RAERLGSINTEFYLYAETDMNEISRAIEHISPDYVIIDSIQTMTQPDITSVAGSVSQV
                     RETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILR
                     AVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLDGATGSAIVVAMEGTRPILVE
                     IQALVTPTMFGNAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEP
                     AIDLAIAVSIASSYKEKGTQPTECFIGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYL
                     PKNNLGNWEAPKGIEIVGVATLAETLKRVFR"
BASE COUNT         3464 a         2863 c         2376 g         4478 t
ORIGIN      
        1 ttgattttat tttaatataa gttgattaaa agagagcatg atttgtttta ttttctcact
       61 aatttttcca acacaactaa gaataccata aaagctgatt ttggaggagg ttcctactac
      121 aagtctaaaa cgaaatttaa gacttttcct cataaaatac tggtttattt tggtctcatg
      181 taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag tacgtctttc
      241 atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc aaataaactt
      301 tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata ttgtccaagc
      361 attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc agcgaatcca
      421 agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc tccgctgaag
      481 tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc atcgataatt
      541 ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat ttggaacaac
      601 agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa tgtgtctaat
      661 cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc actttgtttg
      721 atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg agggaaattt
      781 tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat cttaccaact
      841 acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata caagtcttga
      901 gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa tgggtgtgta
      961 ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc ttcatacatt
     1021 tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt gaagtaaggt
     1081 gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt cgtagaaaac
     1141 agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg tttcccaaat
     1201 aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa atgggcaatt
     1261 ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt atcgatcgaa
     1321 ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg caacaaatac
     1381 acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg ctcatattct
     1441 gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt cgatttgctc
     1501 agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc attcttcatc
     1561 agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg ccgcttgcgg
     1621 agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag acagttccaa
     1681 ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat gaagtacttg
     1741 atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga acgtatccac
     1801 gctactagag gcataataag ctagactttc tttctctctg acattcatga aaagtttaga
     1861 gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt ctggttcatc
     1921 gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg gctcacgtac
     1981 ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg gataaactgt
     2041 ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg gatcaacatc
     2101 gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt aggacgctag
     2161 cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga tcttttgttc
     2221 cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt gtttatcttc
     2281 cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg tcaactgatc
     2341 aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat ctaccgcttg
     2401 agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc ctgcgtttag
     2461 ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat aaagctctgc
     2521 aagttgcgca cttaactttg tctgatccgg ataattcaaa ctatttgttt ccaacatgct
     2581 gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa aacggataaa
     2641 aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc cttctgccaa
     2701 ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac tcttttaatg
     2761 ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca ggcaattcgc
     2821 cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt atcaaaagaa
     2881 acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat actaaagaca
     2941 aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc atgcgtggag
     3001 atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc atcgtaaacc
     3061 aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg atcacaggta
     3121 cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa ggcgtcacat
     3181 ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt tttgtcttat
     3241 tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa gagcaagtgg
     3301 aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat cttcttgcca
     3361 aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc gatacttcta
     3421 aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc tttttttaga
     3481 ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat cttccaaata
     3541 cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt attcttgtgc
     3601 gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg tttcttgaac
     3661 aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac gtccaaaatg
     3721 gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat cagtatcttg
     3781 aaaaaacgta tgccaagctt gtccatcaat gtcatcaact tcttgataag gacactgttg
     3841 ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg ctgttgacaa
     3901 ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt ttttattctt
     3961 agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatctcagg ctcgtttgct
     4021 ttttaaactg cttcagttaa aaattcgatc tcaactgtcc gagtaagtac atctgaatag
     4081 ctgtaagata ctcgttcaaa agaattttct tcaggatcca aatcaacaac gaatactgaa
     4141 ggataggttt ctgttaaaac gccgcggcgt tctgtttgac gctttcttcc tgtctgagca
     4201 accaacatga ttgggctgcc gatacgacct tctaaatctt tcttgattga agctaaagtt
     4261 gttggcatac agttcacctc tactatcaac tataccacat atttttaaaa ttttcaagtt
     4321 taccataaaa aaaaagaaat gcaataaaaa taaataagaa gctcattaag atttttctaa
     4381 attattcctc attataaaat ttcaagtgtt tttttatttt gagttttact ctcccgtaag
     4441 cgctcattac ttgcttgtga tccattgcta aacattcagc catgtctaca aaatcaacgc
     4501 cttgcatata ataatgaaaa attttagctt ctatttttga aaactggatt tcgcaatttt
     4561 cgattgcttc ttttaataaa agatgctgaa tcgctaaagg ttcttgcaat gtgatctctg
     4621 ctaaaaagtc tggtccttct gtctctattt tttggtcgat tgagacttca tctttttgat
     4681 cacgacgctt cagcgcgttc tgcttcctca acagagaata gatttgattg cttataatgc
     4741 ttttataata acttccaaag ctaacacctt tgcttttttc gtaagttctc aatgcctgat
     4801 ataaagcgat ccttgcttct tgttcccaat cctcagaaac ataatttctt aaataatagg
     4861 tgtttttcaa acgtagtaaa agtggaaaat atttttgata cagggcagca aatgcttctt
     4921 cgtcaccatt acgtgcagcg cgcaataact cctcagacat aaaataattc ctccataaat
     4981 tcgtttatgg aggaatctta tcatcaatga ttcatcgaac tgattagaac gatatgttat
     5041 ttattttttc tcattaaatc gtccaatttt tgggacaatt tttttaactg ctcttcattc
     5101 catggagaat tacgtcggaa atcaataaaa tgcatatctt tattttcttg ggctattgtt
     5161 ttttccgttt tttttatcgt cttatataat tcataagcag aagttcgtaa tgctccttgg
     5221 gaaaagatca tccactgttc tgctaaatcg cttgtcgcca ctgtcacttg tgtcaaacgg
     5281 tcattcagct gaccagcgat tctctcgata taactgtcag ccgtttcgtc ttctttcgta
     5341 aaaacaacat ccaactggta tttcttataa ttttgttgga tgcccggaac tagttgggca
     5401 tcgaagacaa cgatgacttc cattccttca tatttcgcat aatttgataa acgatgtaaa
     5461 agagcttctc ttgcatccgc cattttgtcc tgctttttaa gctggacaag ttccggccac
     5521 gaaccaatca tgttgtagcc gtctacaatc aacagctgtt ttttcatggt ctactccccc
     5581 tgacgttggg aaaaagcctg atacataagc aaaccagcag caacactggc atttaagctc
     5641 tggacgtgcc cggtcatcgg gatcgtcagc aattcatcca tttctttttt caatcctgga
     5701 ctcattcctt tgccttcatt accaatgact aaagcaattg atcctttggc attccatttt
     5761 tggaagtttg tgccggtcat atctgtgcca aatacccaaa atcctgtttc tttgagctgt
     5821 tgcacagctt gtaccaaatt cgtcacgcgt gcaacaggga catgttcgac tgccccagtg
     5881 gaagtctttg ttacgatagg tgtgattcca actgctcggt gttttggtat gatcacacca
     5941 tcgacgccac ttgcgtctgc tgtccgtaaa atcgacccaa aattgtgtgg atcttcgatg
     6001 ctatccaaaa tcaaaaagaa ggggtcttct tttttctctt ttgttcgttc aatcagttgg
     6061 tcaagggata aatattcata aggtgtaatc gcaagaacga ctccttgatg cacgccgtga
     6121 tcgctcaaag tttctaattt ttgtttagga acccatttga ctggcacagc ttgttcacgt
     6181 gcgatttctt tcagttcttc tactttttct cctcgtgaat cttcttgaag aaagagttta
     6241 ttccctcgcc cttgttgtag cgcttctact gcggcatggt gcccaaagac gaaattatct
     6301 tctaatgctt cttcattttg atcagaaact gactgatttc gtccagtttg ctttttattt
     6361 gaatatctat tttgtttttt gtcttcaaat ccacgtttcg attgatgttt gtttgttctt
     6421 tctttaccca tttttttcac caactttctg aatacaccag cggatcaatt cttccatccg
     6481 ctcggtttgt tttgtcaaat gcagatagcc catgagcgct tcgaaccctg ttgcgacccg
     6541 atacgtcgta acatcagcat tttttgcgct cgtatggctt ttagcatttc ttcctctgcg
     6601 ataataaagt tcttcttctt ctgtcaaaag atgttcttcc agcatttgct gaatcaaaaa
     6661 ggcttgtgca ttcgcagaaa cataattcgt tgccgcacga tgcagcttat tgggttttgt
     6721 ttctccttga tcaatcaggt ggtcacgaac atagatttcg taaatcgcat ctccaacata
     6781 agccaaggct aaaccgttca attgtgtgta atctctcatg cttctcttct ccatcgggtt
     6841 ccttgcggtg tatcttctaa taagatccct ttttctttca acaaatcacg aatttcgtcg
     6901 cttcggacaa aatcacgatt gcttcttgct tgatttcgtt cttcgatcaa ttgttcaatc
     6961 tcttcatcta ataactcttc tgctgtaaat aaaataccga agatcgaaag ccattgcgag
     7021 aataaagctt ctccttgtgc caagacagcg gcagaaactt tcggctggtc tagataagtg
     7081 ttcagccact tagcaagctc atagaccact gtgatcccat ttgctgcatt aaaatcatcg
     7141 tccatttctt cgatgaatcg ctgttctaaa ttttctaaca aagcgatttt atccgcatca
     7201 tctgccaaat tctctgaggc agtttcttgg cggaatttca aattgtcaaa cgtattcttc
     7261 agtcgttgta aattggtggc tgcttctttc atcgtcgttt cactgtagcg gatcggacga
     7321 cggtattgtg tggtcgccat gaagaaacgc aagacttgtg gatcaacgtt tttgatcatt
     7381 tcatgtacag tgatgaaatt ccctagagat ttactcattt tttcatcgtc ttcgccaatc
     7441 gttacatagc cattgtgcat ccagtaatta gcgaattttt tccctgtttt cgcttcactt
     7501 tgtgcgattt cattttcatg atgaggaaat tctaaatctt gcccgccacc atggatatcg
     7561 atcgtttctc ctaaatgttt tgttgccata acggaacatt caatatgcca accgggacgt
     7621 cctttgcccc atggtgaatc ccaagaaatc tcgccaggtt ttgcttgttt ccataaagcg
     7681 aaatctaatg ggtcttcttt ttgctgctgt tcttcccctg tacgctgact tgctccgact
     7741 tccagttcat cgatggattg gtcacttaat ttcccataat tttcaaattt acgggtacgg
     7801 taatacacat cacctgaaga ttcataagca tagccttttt caaccaacac ttgaataaat
     7861 gctaaaatat ctggcatatg atccatgact cttggatgca gtgtagcagg ctcgacattc
     7921 aacgctttcg tatcttcttc aaatgctcgg atgaaacgat cagcaacttc tggtgctgta
     7981 atacctaatt cgtttgctgt acggatgatt ttatcgtcta catccgtgaa attcgatacg
     8041 aaatcgactt tatagcctcg atattcaaaa tagcgtcgaa ttgtatcgaa agcaatggtg
     8101 ctacgagcat ttccgatatg gatataatta tacacagtcg gtccgcagac atacataccg
     8161 acatgtccct tttgtatagg gatgaattct tctttttttc tggtcaatgt attgtagatt
     8221 tttatcattc tgatctcctc actttttctc ctttgattcg aacgactttt gctggtactc
     8281 ctaccgctgt agcgtcgtct ggaacatctt tcaacacaac ggcacctgca ccgattttgg
     8341 cgttttttcc gatcgttacc ggtcctaata tctgcgcatt cgcagagagc atggctcctt
     8401 gtttaacagt tgggtgtctt ttacccgttt cttttcctgt gccgcctaat gttacgccat
     8461 ggaataaaat cacatcgtcc tcgatttctg cggtttcgcc aatgacgatt cccattccat
     8521 ggtcgatgaa cacgccagtt ccaatcgttg ctcctggatg gatctcaaca ccagttagga
     8581 aacgccagaa ttgagcattg atcttcgcca ataaaaagag ccggtgctga tataaaaaat
     8641 gagagaaacg atgccaaaac aaggcatgaa aaccagggta tgtcaaaacg atctcagctg
     8701 tcgaacgtgc agccggatcg tttctctttg ctgcatcaat cgctcgggta aaccaactca
     8761 tgactttcca ctcctttcgt aagaatttta tgtaggaaat catcttctta taaagaaaag
     8821 aagcgccttt taaataagtt tccttatcaa aagacgcttt ccgcgtggtt ccacttttct
     8881 tcataatcaa tgattatctc tgacagctaa cgcctgttat acgttcttgg ctactttgtt
     8941 tcctataaaa cagtttttcc tgttttctgt gtttcgccaa gaccactccc agaggcattt
     9001 cggcaaccat tcattgatac cttgcaccac ccggtatctc tcttgtaatg atcagctgct
     9061 tacttttttc tgttcatcgt gtttagttca gtacttgatt taaatgtgct aatgattttt
     9121 cacgaccaag caattcgatc gtttcaggca attctggacc atgcatttgt cctgatacag
     9181 ctacacggat tggcatgaaa agattttttc cttttacgcc agtttctttt tgaacagctt
     9241 tgatggcagc tttgacagtt ggagcatcta ctacttccat ttgttctagc tgtgctttga
     9301 atgcgtggag aacagtagga actgtttcac cagcaagtac ttcttttgct gcttcattca
     9361 atacaggatg ttcattgaaa aatagcgtag acagatcaac gatttctgca gcatagctca
     9421 tttgaggttg atacaggcta acgatcttct tcacccattc gattgtcttt tcatctgggt
     9481 tttctgctac acgtccatca gcgattagat aaggcagaca catgtcagta aatgcatcta
     9541 gatccattgc tttgatgtaa tggttgttta cccattgcag ttttttcgca tcgaaagctg
     9601 cgggtgattt acttaggcgt tttggatcga acattttgat caattcttgg tgttcgaaga
     9661 tttcgtcttc gcccacaggt gaccagccca ataatgcaat aaagttgaac attgcatctg
     9721 gtagatagcc taattcgcga tattgttcga taaattgcaa gattgattcg tcacgtttgc
     9781 ttagtttttt acctgtttca ctgttgatga tcaatgtcat atgtccgaag gttggtggtg
     9841 tccagccaaa tgcttcatag atcatcagtt gttttggtgt attggcaata tggtcatctc
     9901 cacgtaaaac gtgagtgatt ttcatcaaat gatcatcgac cgcaacagca aaattataag
     9961 ttggcatacc atcgcgtttt tgtatgacaa agtcgccacc gatattatcg gattcgaacg
    10021 aaatagcgcc tttcaccata tctgtaaatg cgtattctgt attgcgaggc acacggaaac
    10081 gtacaacaga ttctagacct tgtgcttctt tttctgcttg ttcttcaggt gtcaagttcg
    10141 cacatgttcc tgcgtaatga ggcatttctc ctcttgcgcg ttgtgcttcc cgttctgctt
    10201 ctaattcttc ttctgtgcaa tagcatttgt atgctcgatt gctagcaagc agctgatcaa
    10261 tcagcggttg atagatttct ttacgttcag attgacggta agggccgtat tctccaggat
    10321 tttcaggaga ttcatcccaa ttcattccca accaagcaag gttttccaac tggctttttt
    10381 cgccatcttc gatattacgt ttttggtcag tatcctcaat acgaataata aagtctccat
    10441 cattgtggcg ggcaaacaaa taattgaata atgcagttct tgcatttcca atatgcaggt
    10501 gcccagttgg acttggtgca tagcgtacgc gtactttcgt cattttcttc ctcttctttc
    10561 aaactagact ttgactaaca gcaaaaattc cgtcagttac ggcttctcaa atatcattta
    10621 tttaaagcca ctcagcacaa attttacctt ttctaaggta aatagtaaag ccaactggtt
    10681 gaatttcctt accagttggc tgaatttctt ttttattttg attgtttagt ctttttctct
    10741 gtctcttttt tattgtcgat tcctcgtcct gcatgtgcag gtttagcaaa gatcatgcgt
    10801 ccagcagctg tttgaagggc acttgttaca acgacttcga tacgttcatt catgtaatgc
    10861 tgtccatctt ctacgacaac catcgtgccg tcatcaaggt aggctactcc ttgttggcgt
    10921 tctgtcccgg cttttactac tagaacattc atcgtttcac cagggatgac gactggtttt
    10981 acggcatttg ccaacgcatt gatattaaga acaggtacat tttggaattc agagacttta
    11041 ttcaagttat agtcattggt tacaatgaca ccatctaaaa gcttagccaa tttgatcaat
    11101 ttgctgtcta cttcactgat atcttcaaag tctccatcgt acatttcgac agatataccc
    11161 tcttcttttt gcaaggcatt taggatatct aatccgcgac gaccacgcac acgtttcaaa
    11221 ctatcccctg agtccgcgat gtattgtagt tcgtaaagaa caaaattagg gatcatcaag
    11281 gttccttcta agaatccagt ctttgtaata tcataaatcc gaccgtcaat gatcacgctt
    11341 gtatccaaga ttttatattt atggaaattt tctcctgatt ttcgttctaa cagttgactc
    11401 tcaacttgac tagctgtttc ttgctcgatt tttttgcttc gtgaaccaaa aactttcttc
    11461 cattcttcga ttcgtgtcgt tcccattctc aatcccagat agcctaagat gatcatcagt
    11521 aaaatcggca atacactatt gacgaatgga atttccatat tgtaaagagg ggtcgagatc
    11581 acaacaccta aagtcaatcc gatgatcgca ccgatcgcac cgaagagaag ataagtcaag
    11641 ctgacctcac ttagtttttg ttctacttgt ttgataatag ctgagatatg tttggccaac
    11701 agcaaagata ataaaaagaa aataagtgcg cctaacaggc tgttggtaaa tgtattattc
    11761 agccatccgt ttgactgttg accgattgcc tgccaagctg agggtaaaaa tgaaataccc
    11821 aagcttgcgc cgacaatgat catcagcaaa gtaatgacac gtttttgcat cgtttttcct
    11881 ccgtttcgac aatcatttat tagttagcgg aacacacgct tcaacgtttc agccaaagtt
    11941 gctactccaa ctatctcgat tccttttggc gcttcccagt tacctaagtt gtttttaggt
    12001 aggtatactt ttgtgaagcc gagtttttgg acttctttga ctcgttgttc aatatgactg
    12061 acacgtctga tttctccagt cagtccaatt tcaccgatga aacattccgt tggctgtgtt
    12121 cctttttctt tatagcttga agcaatactg acagcgatag ctaaatcaat cgctggttca
    12181 tttaatttta ccccacctgc agcttttaag taagcatctt gattctgaag aagcaaccca
    12241 gctctttttt ccagcactgc catgatcaga gatacccgat tgaaatcaag acctgttgtt
    12301 gttcgtttgg catttccgaa catagtaggt gtgactaatg cttggatctc taccaaaatt
    12361 ggacgtgtcc cttccattgc cacaacgata gcagagccag ttgcaccgtc taggcgttct
    12421 tctaagaaaa tctgtgaagg attagcgact tcttccaatc catgctcatg catttcaaaa
    12481 atcccaatct cattcgttga gccaaatcgg ttttttactg ctcgcaaaat ccgaaaacta
    12541 tgatgttgtt caccttcaaa atataacacc gtatccacca tatgttctaa cattcttggg
    12601 ccggcgatcg agccttcttt ggtaacatga ccgacgataa aaatggctat cccatttgtt
    12661 ttagcgattt tgagtagttc tgcagtggtt tctcttactt ggctgacact tcctgccaca
    12721 cttgtgatgt ctggctgcgt catcgtttgg atggaatcaa tgataacata atcaggggag
    12781 atatgttcga tggcacgact gatttcgttc atatctgttt cagcgtatag ataaaattct
    12841 gtgttgatac ttccgaggcg ttccgcgcgt aattttatct gctctgcgct ttcttcgccg
    12901 gatacatata agacttttcc gcctatggct gctaattgct gagaaacttg taacagcaaa
    12961 gtggatttcc cgatacctgg atcgccacca atcaagacca tagaccctgg caccacacct
    13021 ccgcctaaca cacggttcag ttcttctaat tttgttttga tccgaggttc ttttttaggg
    13081 acgacgtctg cgatttttgt tggttttgct ttttctcctg tcaaacttgt ccgagttctg
    13141 cggtccgttg tatcttgttc gatttcttcg accatcgtat t
//
LOCUS       DACEOE010000066        13102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEOE010000066 DACEOE010000000
VERSION     DACEOE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.068"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(61..972)
                     /locus_tag="IW774_001562"
     CDS             complement(61..972)
                     /locus_tag="IW774_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1153912.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1375..1953)
                     /locus_tag="IW774_001563"
     CDS             complement(1375..1953)
                     /locus_tag="IW774_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1153913.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(1973..2641)
                     /locus_tag="IW774_001564"
     CDS             complement(1973..2641)
                     /locus_tag="IW774_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153914.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(2656..3726)
                     /locus_tag="IW774_001565"
     CDS             complement(2656..3726)
                     /locus_tag="IW774_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1153915.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(3897..5261)
                     /locus_tag="IW774_001566"
     CDS             complement(3897..5261)
                     /locus_tag="IW774_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1153916.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(5258..6817)
                     /locus_tag="IW774_001567"
     CDS             complement(5258..6817)
                     /locus_tag="IW774_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1153917.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(6873..9335)
                     /locus_tag="IW774_001568"
     CDS             complement(6873..9335)
                     /locus_tag="IW774_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153918.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGIPLQFDVTANVAKMTENGDKYDTSPN
                     KILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGGNLNPGA
                     YAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLTMGLTEG
                     RFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQGGLLSP
                     VALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIHYRLYSK
                     GTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTNMYPSFA
                     YKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQENVTFT
                     LTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLSKIGDTI
                     HLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTILVPKQEL
                     TLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLTSLFKND
                     QGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLLEVNSST
                     NAQPGATYQGMVTWELTAGPTS"
     gene            complement(9319..9654)
                     /locus_tag="IW774_001569"
     CDS             complement(9319..9654)
                     /locus_tag="IW774_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153919.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(9668..10702)
                     /locus_tag="IW774_001570"
     CDS             complement(9668..10702)
                     /locus_tag="IW774_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1153920.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKVETT"
     gene            complement(10792..11472)
                     /locus_tag="IW774_001571"
     CDS             complement(10792..11472)
                     /locus_tag="IW774_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1153921.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(11756..12859)
                     /locus_tag="IW774_001572"
     CDS             complement(11756..12859)
                     /locus_tag="IW774_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1153922.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         3762 a         2695 c         2246 g         4399 t
ORIGIN      
        1 taataagccc ggccaaacga aaaatatgag gagctatttt tgtttggctg ggcttattta
       61 ttactgaaaa taagtggtca atgcttgtgt caaacttgca gcaatctgct gctgataact
      121 gtctgttact aattccttta aatcactatc attattcata taacccatct cgattaaaat
      181 agaaggttga agattttctc gaagaacttg atagttccca gtggagattc ctcttgaatc
      241 cagaggcaga tcagtgaagg agtcagcaat tgtctgtgct aatgtttcct cttgtccata
      301 gtaataatat gtcgtgattc cagttgcttc attcgcatat tccgtagagt ctgcgtgtaa
      361 actgataaag acatctgcct ttttttcttc actgtagtcg catatatctc caagctgtac
      421 taattcatct gtagtatggg tcaaataaac cgttgctcca gcatcctcta gcgccttttt
      481 tacttttatg gctgtactta atgttatatc tttttcttct gtttgttcat cattgcttaa
      541 tgccccagta tcatttccgc catgcccagg atctaataca atcactgcat cagatagctc
      601 ttccggtttt gtttgtatat tctttgtgac tttggttcca tcttcgaggg ctaaatatcc
      661 agtatagcca tcatttgtct tgatctgata agcatttttt gtttcagaaa gaaactccgc
      721 cagctctccg ccgtttatag ttgcaagtgg ggtagttagc gagtcatctg tatatagtgt
      781 acggtcattt tttccgattt tgattaatgt ctcttgttct gtatcatcaa atgattcctt
      841 tgtctttgta accgtactgt tcgtactgct gaccgtttgt ccaactatct ttttttgacc
      901 aaaaacatct atttgaaaac agataataat gataatgatt gcaaatacaa aagctccaat
      961 tatttttttc attcttgact ctcacctcgt agatccagac taagggtcaa aacttaaaat
     1021 cagcttaagt acgcaaaaaa ggggctgtaa aagatataac cgtagaaaat aacattactg
     1081 ctaaaatgat ttttttcatt tttggcatat cctgaaatct ccttttgact attcgtggtg
     1141 attcatctac ccaataaata gtttccttca ttctacaaac aattaatttc ttcgttatta
     1201 aattaaaaca cttcctggaa caccgacata ataaataaaa atataccctg ataatcatga
     1261 gagaataaca aatttgtatt tatgaaaaac tacctatata aaaaaaacaa gatataacga
     1321 tcttcgtcat atcttgcttc ttcacctaac tatttttctc cctatataac agctttacgc
     1381 ttctcctaat acaccattca aatataaatc gatcaattgc gccacattta gcggttcttc
     1441 cacaggtctt ttcgaccgag tgatgtaagg tgctaacact aagaaaaaat gccttgctgc
     1501 gatttctttt gaaatcccat ttcttagcac agattggttt gctgaaaaga tttcttcaaa
     1561 aggttcacga taatttttct tccatagaaa gaaaagatga tctcttgttt cctttgaaag
     1621 attgctttca agatcgtgaa gcatcagaaa caaatcgacc agatgtgtct caatcaaaaa
     1681 ctgcgccatt tttgtcaagg ttacttgaaa tcccgtttcg cttgtttgaa cgatttctct
     1741 caaagctttt ccaacatctt ttaagtggtt ttctaaaact gccgtatata gtttttcttt
     1801 atcaccgaaa taatgataga gattgggttg ggtgatccca actttttgtg cgatctctct
     1861 tgttgaagtg ccgagatatc ccttggacat aaacaagtca gtcgcttctt tcaaaatttt
     1921 ttgctgtgta tctttgttgt atcgatggac catttttcca cctgcttttt ggttattctt
     1981 tttctttcaa tacaccatct tccatcataa atattctatc gcatttgtca atcagccgtg
     2041 tatcgtgagt aaccatgatc gttgctttat ttttgtcttt tgattctttt gccaagatat
     2101 cgactacttc tcgtgccttt tctgaatcca aactagcagt tggttcatct gctagaataa
     2161 tggacggatc attgtacaat gcacgagcga ttgcgaccct ttgccgttct ccaccagata
     2221 gttcttctgg atacttgttg atcaacttat ccacgccaag ctgctcaaat aattcttttg
     2281 cttctttttg acgatgttca tgtttgatct tatctactaa taataactgc ttttttactg
     2341 tcagaaaagg aaccagattg gaagcttgca aaataaagcc aatttcttta aaacgtagtt
     2401 taacgcgttc actttctttt tgattactga attcttgatc attgataaat acagagccac
     2461 tgctaggcgt ctgcaatccg cctgccaaag tcaaaaaagt acttttacct gaaccagaag
     2521 gaccgatgat tgcaacaaat tcccctttat cgatactcat ggttgtttct ttcaatgctt
     2581 taactacttg gtcgccatct tggaaacttt tttctacttc tttaaattct attgctttca
     2641 tgctcttcct ccttcttatc caattgcctt caacggatcg atttttacga ttgtccgaac
     2701 agaaaatagt gcgcctaaca cagctactac cagcatcaac aagctaatga cgccaaaaaa
     2761 cagccaattg ctttggaatg gaaccgcatc aggtagaaca agggatgttc ccactgttcc
     2821 taatagacca agaagaatac caacaaatga taaaatgaat gtttgtgcca ctacagaacg
     2881 cgcaataaat cctcctgtga tcccttgtgc tttcattaca ccaaagatat taatcttttg
     2941 catcgtcagt acataaatga aaatcccgat cacaatagcc gcaataacga tcaaaaatcc
     3001 gatcataaat ccaaaagtca atacttgcgc attatatcca ggtaattcat tgatgaattt
     3061 agaaattttg atctgttcta aatcttcagg taaattactg attttacctc tgacaactat
     3121 tgcgttaatc cgtgcatttt cacttgtatc ttctttttcg aagcggatct cttggtaagc
     3181 attgatggtt gtatagagaa ctggtgagac attgaatttg gcatgatccg taaagccagt
     3241 tagtttaaac gtcttgtcac taccagataa cttaactgta tctcctacgg ctagaccata
     3301 ttcttctttc aaactaatat cgcctactgc ctcatcatcg ttatcaaaag cttttccttc
     3361 aacaagattg ggcatgatga attgattttt atcgataccg aagaagctaa cattgatttt
     3421 tccttcttct gtactgtctt tactagtcac gactccaggt gtctgggcaa gatacgccac
     3481 tttcccacct tcgacttctt ctgctatctt cttagtaatc atggacatcc ctagatttga
     3541 atttgcatct ttcgaaagca caatcgaatc tgcctcccac ttgtcgactg ctgttcgatt
     3601 atcttgagct aagccataag ctaacccagt caaaaaaaat accaggtagg cgatcaaaaa
     3661 catcacccct gcaactaacg cataacgtaa ctttgaatgc ataatttcat ttaatgctaa
     3721 aaacatttaa ctcctcctcc tgaaataatc attttataca acaattttat cacttgataa
     3781 atttttggcg aatattatgc tcaaaaaatt cttccgctat ttcattatat gaatttttta
     3841 ttctcgattg attaatattt attatatccc cacacaataa aagaccgtga tatttctcac
     3901 agtcttttat ctactctatt gctatttgct ctcttagcta gtactttaaa agctaactca
     3961 ttgaggtagt gaaaacgaga ggtgtgttca gacagaaatc caaaatcctc aactgctagc
     4021 tgtggaatcg tagttttgtg tttcacgata tccgcaagca ggttcaaatc tgataataag
     4081 aattcttggc ttatcagctg tccgccaatt atttctaatg tatccggatg gtagatcagc
     4141 tttgcattta ctgttttgtt ttcttcatcg tattgtggaa atagatcaaa ttctcctgaa
     4201 cacgaaccgg tattttgttc caagaatggc gcttcatctt ctgtcagccc tacagttcct
     4261 aaaaagtagt ctataatgac tgctgctgtt atcttttgtc tgaggtcgta agcggttggc
     4321 tgaccactca aagttagcgc tactgctctt gccatatgta cagctcgagt aactaaaggt
     4381 aaaaagagat gccgtttatt tttgtcaaaa ctgactggga cgatatctcc aatcgcatag
     4441 atgtccggat cacttgtctg cagatattca ttgactatca cagtctcatc ctcatacaaa
     4501 gccacttttt cttttaacaa tgtcgtatta ggacgagttt gggcagaaaa aatgaccata
     4561 tctgccttta ttgcttgaga tagaagttgt acggatacta ctttctcttc tgattttttc
     4621 caatcgatca agaattcatt tggataaaaa ttgatttgct tagtttgcag ctgattttga
     4681 aaactgttga tcatatcctt atcgaaatat cttgataaaa cattcggcat ataatcaact
     4741 agatgaataa tgtatccttt atttctcaaa gcatctgcta attccaatcc aatataacca
     4801 gcaccgaaga tcactacttc ttttgcttct tttaaagcgg caagtacttg cttagattgc
     4861 actagatttt tataggtgta gacattttca gcttcaattt gtttatacaa ggtgacagga
     4921 gaggaacctg ttgctacgat taatttatca taggttatgg tctcgttttt tccttctttg
     4981 gatgcgagga tgagctgatg tttgtctgga tcaagttcag ttactctcca ttcggtgatc
     5041 agttttgccc cactttcttg aagagtccgc atgttatggg caacttcagc taaactacgt
     5101 atctttcctt ttaaataaag gttgattcca tttgaaacat agcctagtcc atctttatga
     5161 tcttcatcga tcagtaaaac ttctgctttc ggttgaagtt ttcttagatt tagggcagct
     5221 gtgatgcctg catgggaagc accaatgatc acgattttca tgttttattc atccttaaat
     5281 tcaatttttc taaataacat tcttttaaaa attggttcaa ataggggaaa tcataattat
     5341 attcgttgct agtgaataca aaaactttag cggacttctg tgacgggtac tctttcaatt
     5401 ggctagtcaa aatgatatca tactgctttt ctttctcgta ttcgactaaa tcatatttga
     5461 tcgtattcag caattgttcc tggatgattt ctttcacgat atccgccatt agcctttcat
     5521 aaggaagatc ataagcaatt tgcagacgta attctaactg cttgatcagc gtccgcaaaa
     5581 tagatgcata gcgagcaaat aattcaaagc gggcattttc ctcgtctact tcagtaaaat
     5641 caacaatagc aaatgtttgt ctgaccaatc tttcacataa aaccaggaat tgtttatctt
     5701 ctacttcagt taaccgctgt tttaatcctt cttggccaaa aatattcatc catcctctaa
     5761 aataggcaaa atgagcgtga ttttgcatca ctgtcaacgc aatcttttgc gaaatctctt
     5821 ctggaaaaac ataaaaagag aaatattctt ctatcgattg tttaaacgtt cgattcaatt
     5881 gagctactac gtcgtctttg tatttttcgc ttatcaaaaa ctcctggaca tgcgagttat
     5941 gtgtatctaa tgtaaagtta ctgatcatga aaagataaaa aaccaacgtt tcattttgat
     6001 tccaaggaaa ggcatattga aagaagtaac gggtcaaaaa tgtatggagg tccaataaaa
     6061 attgtgtcgt ttgaaacaat ccaacaggaa gattgctata atctggtttg gattttttcg
     6121 tacagcgcat tcgcttcttc ataatggaaa gccatatttc aaatttcaat cgtccttctt
     6181 ctgtaatagt aaaactaaac tctttttgca gcagatctaa aaaagtgttt cctgataaat
     6241 cactgtattc ttttttgttt tcaagataag gacgtccaaa ccaaaataat tggaaataaa
     6301 aataacaaat ttgactttct ggaccggtca gccgcccgct ttttattttc agatgaaact
     6361 catttaaaga atcatttaat tccttcactc tgcgagacaa agtagccgaa ctgatattaa
     6421 gttccatgac aagtttctgc cgatcaaatg agccattttc caacagataa agaagtatct
     6481 gatattttgt agaatagcgc aagtagcaat gataataaac atcgactgct gctatatttc
     6541 catgtctgat taataaaata ttcccttctt ggataacaat ttccaattgt ttgatatttt
     6601 ccaagttagc ttgtaaataa aacagatctt ctttcaaggt agcccgatca actgataagc
     6661 gggtaagtac gttattaacg gaaatctctc ctttttcctc taataaaagt tggaaaagtt
     6721 tgatttgccg ttgttcattt ttttcaagca aactgaaaag ttccaagtcc actcctccaa
     6781 gcatttctga ttttaacagc agaagtttcc ttctcatcct tgaaaaagag gatctgtgac
     6841 agttatcaca gatccataca aattctaatc acttagctgg ttggcccagc tgtcagttcc
     6901 catgtgacca tgccttgata cgttgcgcca ggttgagcat tcgtactgct atttacctct
     6961 aagagtaaac catcggtatc gggaaagtca acgagaacct ctcctttagc tgttccatca
     7021 ctctcataaa tcaacgtgtt ttgaccttgc tgaatcaatt gttttgatcc agaatgattg
     7081 aagtataatt ttgttccgct taattcttga ccttgatcat tcttaaataa agaagtcaat
     7141 gtccctgtca gttgccaagg agacgtgctt ggaagccgag tatcccgcac atcaaaagaa
     7201 aaatcgcctt ttgcttcata ataacttgtc ttcaacggtt tggggagatc gttaccaaat
     7261 gtaaaatcat ctggtacatt aactaatgtt aattcttgtt tggggacaag aattgtttta
     7321 gcagatgttt ggacaactgg gttaggaagc ttagcaccat ctgctaatgt aatattgctg
     7381 gaaagtacag ctgcctttgt cagtatgctg acggattgta cagaagtatc tacagtattt
     7441 aattgatagt tgtaagtaag atgaatggta tctcctattt tgctcaatag atctgctggg
     7501 acagaaactc cttgttgaat gccggtatct gcgatagttg taccatttaa ttttaaggaa
     7561 ccattgatat aagtagtatt ttcaggaagt gagatcgtga tttttttgtc ggttatcgca
     7621 tccccagatt ttgttaacgt gaatgtcaca ttttcttgaa tcgtatctcc tgatagagca
     7681 gacaggggat ctcctgtctc aggttgtctg ctaaggtttt ttagctgact atctgcactc
     7741 acagatggaa aattagccac tgttatttga ggaagagtgg tgattccagc ttgttcagac
     7801 tgccatttgt acgcaaaaga cggatacatg ttagtaagta catcgtccac caagcgataa
     7861 tcaaaataat attgccctga agccaatgca ggtagtgtag aagtagctgt atttacaccc
     7921 ccgttatttg attggatcgt ttggaaaagt tgatctgtcc atcctgtcgc taatttttgg
     7981 gttcctttcg aataaagcct gtaatggatc gaaccctttt ttagttcagt tgctgaccaa
     8041 ttggccgtga cttggtacga tgcagaagaa aggagctttc cccactgact atcggctgtt
     8101 aagtttgttg gtaaaaccgt tccgtctagc ttgtactgct ccaatacctt taaagcaaca
     8161 ggagaaagca atccgccttg cgcatctttc ccactgtaca cgatattggc tgtctgattc
     8221 aatccacttg tgcttgctgg agtatgagtc agtattgctc cttgtgtaac gttttttcct
     8281 gtaaaatagt tgactacgcg atcaaagcga ctaaatggca actgatcaaa acgtccttca
     8341 gtcagcccca tcgtcaatgt ccgactattg gtagagcctg gaaggttctg gttatttcca
     8401 tccacgtatt ctgtggtatc agcaaatgta ttatattttc ctgttgaatc acttgttgag
     8461 atgtaactat tccacgtatt tatctctgca cttgcagaaa atgcatatgc tcctggattc
     8521 aagtttccgc cagttaattt aggataaaat ctacctgcag ctggagctaa aatcgaattg
     8581 aacatacgat cttggataat attattcgaa tcataaaaat caacagaaaa aacggccttt
     8641 ttagacatag aaggttgtaa tttacccttg gtcaaaattt tgtttggact cgtatcatat
     8701 ttatctccgt tttctgtcat cttcgcgaca ttagctgtaa catcaaattg aagcgggata
     8761 cctcgcgtaa taatagcaat aatatctgcc aatagagtat ttgtatccca cgaccccatc
     8821 ccaaaacgca aggtatggct tcctgctgat aataaactac cgctagtaac cttgatgggg
     8881 gcaccggcaa tatacaagta ggcgttgttc cagtcaattg cttttaatac atcactgctt
     8941 gatacatgtt ccggcaaatt gatgatcgcc acaaatgcaa atccttgtgc agtgatattc
     9001 accaaatcaa gtacatttgt tagtttcaca tggctttccc aatgaaaacg gaccgtgcca
     9061 cctttccaag gatcatttga accatcgttc aaataagtag cagtcaattt aggcaattgg
     9121 ttagccccag ttagttggac aagcttcccg ccattgactg cactacgtcc accatttgga
     9181 ggtacgatat tttcatacgt gtatggatca taagaaatgg caggaggcgt tgctccaaca
     9241 gcttctgccg aaggtgaaaa gataaatata cttaaaacaa aaagcgaagt aaaaaagaag
     9301 gagcgaaaaa atgaaaattt attttgtagc aacatgttca tcctcctttt cctttcttct
     9361 ccataacaac aaccacgcaa gaagccataa ttctatacca atgatcaaat aaagaaaacg
     9421 gatcttttct tcattcgtct ccgggaatac gccatgttga ttagattctt tataaatcgg
     9481 tagctgtgtc tgctgttttg atgcgccatt gactgaacca cttggatgcg gaactggact
     9541 tagatcataa aatgtaatcg tacctgaact tttggtatct gcttggactg gctcttgcca
     9601 gctgaagaac agcaaacttg caataatccc tcccaataag atcgatgttt tcaacgaaat
     9661 cacctcatca tgttgtttct acttttttct ttttcttgtg gacccacaaa aacagcaaaa
     9721 tcaaccataa cactagaact gcagcaacca atcctgcata aagccatggg aaagattgtt
     9781 cttgtactgc aaaaacggaa ttttcattta ctttttgtgc tttttctttt ttgatcgtaa
     9841 agttttgtgt aaatcgccat tcattactgt cgcctgattt tgctatgatt tctgcgcggt
     9901 aatcgccggc ttttgcttta tcaccagcta aatcaagatt caagtgaaaa ttcgaatttg
     9961 gtgcaactaa aaattcatta gacgtatttt caatataaag ctgattttca taaaagatct
    10021 tgctcgtaaa tatcaaattg ctgataatcc taggctgagg attttggaaa cgaagctgaa
    10081 cagttggctt tgcgttgacc tcgtccaaat ctcctgataa aagtttcaga tccggttcta
    10141 tcgtctcttg actttcttgt aaaacgactg cgagattata agcaatacga tttttgattt
    10201 gtgctccttc tgtagcattt tctgtttcag cttcttcttt aggtgaaaca tgaatacccg
    10261 ctaatacacg tcccttaaag ctttttgctg ggattttcac ttctataggt atagttgttt
    10321 ctgcatgtgc tgatacattg atttcctttt tcgcaatgag caaaacgtca cggacatcaa
    10381 acggcaatgt ctcatctaac gttgggtttt tttgactata atcgatcgtt ccaccatcac
    10441 tcgttaccgc cggattgaca gagacctcaa atgttcgttc ttcatctgaa gaattgctga
    10501 taattacttc tagtgtctga ttttgttccg gtgccactaa taaatcaaaa taaccgattg
    10561 ctttatctat ttggttctct ggaaataccg gtttgattcc aaagtctcct gctgaatcct
    10621 ttgcttggct gattgacgga aaaatgaaaa atagacttaa aatagaaaaa aacgaaacaa
    10681 acagtcgttt actttgattc attgtccacc acatcctttc aacaacaact gcggtaaaaa
    10741 atccttcacc tatagtattt tcaccgcagt ttgttgaatt tatttcatac tctatgctac
    10801 tgggccagcc acgaggttcc aagtcaaatc agccgtgaat gttccagctg ctgctttcgg
    10861 cactttcagt gtgatatttg ctggatcata aacagaaagc catgttccca gaccttgatc
    10921 ttttgctgct gtaaatattg gtgtcgctgc acttgcttta ctcaacccag ttacagtttg
    10981 actagttggt ccagtggatt cgttatctga cgtactttta acagtggatg ttccaaaatc
    11041 taattcagcg ccttgcaaaa cttgtgaacc gtttacaaaa tctgaaaccg aaactgtcac
    11101 gttccaccct tgtgcttggc catctgcacc tacacctcga cgatctgata cttggagata
    11161 aggtgtatcg ttttttgaag cgtctacttg atacgttttt gttccgcttt caatctcgtg
    11221 tgtaccaaat ggcaattcag gaactacatc caatgttaaa gaacctgtct ggccagttgc
    11281 tggattttcc ggatctgctg gctctactgg ttttgaagga tcagtagggt taagaggctc
    11341 aacggtattt gttggttcag taaaggtaat atctcctttt gaagtcgctt gaccctcagc
    11401 aaaagctggt gttgccaagc cgcctagaag aagtgttgaa agtaataatg tcccaatcat
    11461 gttctttttc ataataaatc actccttttt cttagtgacc taagtatagt agagacagta
    11521 tgcaaaaaat cctcatgaat tcatccgctt acttgaaatt atgacccttt ttgtataaaa
    11581 gagaaaaaaa caataaaatc gtttacatga atattcttcc ttttttatta agtatgaaac
    11641 aatatttcaa ttttattttt tcgtttcaaa ttattctatt agtgacatcc ttgaactata
    11701 aaaataggag tacggcatca tctgccgcac tcctattttt ttcgtcattt atttcttaaa
    11761 taaaacgatt ctcacgcaat tttatttttt tgatatcttc tactgtttgt gttccagcta
    11821 attgcatgac catttccaat tctgttttaa agaaatcaaa cacttgatgg actcctgtac
    11881 ttcctcctaa agacaatcca taaataacgg gtctgccaat agcaacaaga tcggcacctg
    11941 atgcgattgc tttgaaaaca tgttgtccac gtcgtacacc gctgtcaaac acgattggta
    12001 cacgtttatc tactgcttct gccacatatt gcaacgaatc aaacgcagca ggccccccat
    12061 ctaattgacg gccaccatgg ttagataccc aaattccacc agcaccagat tctaatgatc
    12121 gatagacatc ttcttctgat tgaacacctt tcacgtagac tggcaaatca gaatgagcag
    12181 cgataaattc tacatctttt ggactaagtt tttgtttgga agatttatat acagcatcca
    12241 tcgtttggcc tactcctgat tgataagcct gaacgatcgg cattggcagc gggaaggtaa
    12301 acccgtttct gcggtctgtt tcacggtttc cgccaactgt tgcatccgct gtcaatacga
    12361 ttgcttttgc tccattacgc ttggccactt caagaatatc caaattgatt ccattatctt
    12421 tgctcatata aaattgaaac cattgcggtg ctttctcccc gcctgcttct cttatctctt
    12481 ctaaagtaca agaagcgtaa gaacttgctg tatagatcgt cccaaaatct gctacacctt
    12541 ttgcagaagc tttctccgcc ttcacatgag ctaatccatg agcagcaact ggtgccatga
    12601 tgattggtgc agtcagcatt tcttcgtcaa aatgtgtagt tgtatcgggt aattctacat
    12661 ctcttaatac atgaggaata atcaaacgat gattgaatgc ccgttcattt tcttgataag
    12721 taaataagtc gccagcacca ctgctgatgt aaccataacc accagctgga atcacttgtg
    12781 aagccgcgat ttctaaatct tccatattga taaaatctac tattccttca tttgtacctg
    12841 cttgatatgt tttttccatc tcaatgcctt ctctctataa aatttgaatg atgaacaata
    12901 tgatagcgtt tacacttttt atattactct tcgacattga tacaggcaaa taatctgttt
    12961 acatttcagc cattccagat gtcactactc tggaagaaat gattgtaata aaaatagata
    13021 aaaaaccgat gggactttga caaaagtagt gtcaaagtcc ctttttttga ataaacggtg
    13081 ttcaaaagcc attttctcgg ta
//
LOCUS       DACEOE010000067        12946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEOE010000067 DACEOE010000000
VERSION     DACEOE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.069"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..149)
                     /locus_tag="IW774_001573"
     tRNA            complement(77..149)
                     /locus_tag="IW774_001573"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IW774_001574"
     CDS             530..1099
                     /locus_tag="IW774_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1153923.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IW774_001575"
     CDS             1086..1946
                     /locus_tag="IW774_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1153924.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IW774_001576"
     CDS             2136..2840
                     /locus_tag="IW774_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1153925.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IW774_001577"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IW774_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1153926.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IW774_001578"
     CDS             4677..5993
                     /locus_tag="IW774_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153927.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IW774_001579"
     CDS             5994..6863
                     /locus_tag="IW774_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153928.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IW774_001580"
     CDS             6918..7727
                     /locus_tag="IW774_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1153929.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IW774_001581"
     CDS             complement(7830..8459)
                     /locus_tag="IW774_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ1153930.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IW774_001582"
     CDS             8629..9921
                     /locus_tag="IW774_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1153931.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IW774_001583"
     CDS             complement(9997..10659)
                     /locus_tag="IW774_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1153932.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IW774_001584"
     CDS             10884..11168
                     /locus_tag="IW774_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1153933.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IW774_001585"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IW774_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1153934.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4315 a         2238 c         2802 g         3591 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacg
//
LOCUS       DACEOE010000068        12914 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEOE010000068 DACEOE010000000
VERSION     DACEOE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.070"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            93..422
                     /locus_tag="IW774_001586"
     CDS             93..422
                     /locus_tag="IW774_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1153935.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            412..699
                     /locus_tag="IW774_001587"
     CDS             412..699
                     /locus_tag="IW774_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1153936.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            985..1842
                     /locus_tag="IW774_001588"
     CDS             985..1842
                     /locus_tag="IW774_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1153937.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1843..2391
                     /locus_tag="IW774_001589"
     CDS             1843..2391
                     /locus_tag="IW774_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153938.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2688..3635)
                     /locus_tag="IW774_001590"
     CDS             complement(2688..3635)
                     /locus_tag="IW774_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ1153939.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            3823..5010
                     /locus_tag="IW774_001591"
     CDS             3823..5010
                     /locus_tag="IW774_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ1153940.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            4991..6295
                     /locus_tag="IW774_001592"
     CDS             4991..6295
                     /locus_tag="IW774_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1153941.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            6321..7334
                     /gene="citC"
                     /locus_tag="IW774_001593"
     CDS             6321..7334
                     /gene="citC"
                     /locus_tag="IW774_001593"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ1153942.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            7338..7631
                     /gene="citD"
                     /locus_tag="IW774_001594"
     CDS             7338..7631
                     /gene="citD"
                     /locus_tag="IW774_001594"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ1153943.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            7636..8541
                     /gene="citE"
                     /locus_tag="IW774_001595"
     CDS             7636..8541
                     /gene="citE"
                     /locus_tag="IW774_001595"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ1153944.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            8534..10093
                     /gene="citF"
                     /locus_tag="IW774_001596"
     CDS             8534..10093
                     /gene="citF"
                     /locus_tag="IW774_001596"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ1153945.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            10090..10647
                     /gene="citX"
                     /locus_tag="IW774_001597"
     CDS             10090..10647
                     /gene="citX"
                     /locus_tag="IW774_001597"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ1153946.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            10640..11503
                     /gene="citG"
                     /locus_tag="IW774_001598"
     CDS             10640..11503
                     /gene="citG"
                     /locus_tag="IW774_001598"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ1153947.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            11737..12146
                     /gene="tnpA"
                     /locus_tag="IW774_001599"
                     /pseudo
     CDS             11737..12146
                     /gene="tnpA"
                     /locus_tag="IW774_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(12783..>12914)
                     /locus_tag="IW774_001600"
     CDS             complement(12783..>12914)
                     /locus_tag="IW774_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1153948.1"
                     /translation="RRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         4590 a         1950 c         2537 g         3837 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
      481 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
      541 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
      601 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
      661 catgaaaact tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
      721 aaggacggct tcttttttat cgaaaatctg attcgtctac atgactaaag tcgcgagtat
      781 tctctgcttc tcgataaatt aaataacgtc attactttga ggaaagctat aaatcaatat
      841 atttcaactt ttccttattt atgtactacc ttttacttat taattataag gtagtacata
      901 tatatctttt ttttggtagt aactaaaaat aaatatgtta taatagtatc aaatgaatat
      961 tgctaaaaga aaagaggtag gaacgtggta gcgaagaaat atgtaattgg taattttaaa
     1021 ggtggcgtag gtaaaagtac gtgcgcgcaa atgttagggt ttgaatcagc agttaatgaa
     1081 ggaaaaagaa ccttgataat tgacttggat atgcaaggaa atacttctga tgtgatgaac
     1141 ttaacgcata tgaacttcac taacgaagaa ggaggcggag aaggtgaacc gattgtttac
     1201 gaaaatactg tgactgacgt gttgataaac aatcttccac ctaaagaggc tgtctataaa
     1261 gtaatcaata acctctatat acttccagca gatatgtcct ttgaaatgta tgatgattgg
     1321 atcaaagacc aatataatga ttcattatcc caattcaaat atatgaaaga aaagctagaa
     1381 ccattatttg atgagtttga cgtcatctat ttagatgtcc caccttctat ttctgtatat
     1441 agtaaatccg cgatgtacat tgcggactgg gcaatcgtcg tcttacagac ccaagtaaaa
     1501 tcgatgagaa atgcgatgca atatcttgag tatatggact tttttgtgaa agaatttgat
     1561 acaaatttat atgttgctgg tgttattcct ttcatgttgg aaagcggtga cgcagtagat
     1621 caggaaatgt ataaacaagc ccaagagatt tatggggaac atttgattaa aaatgttgta
     1681 ctcaaaaatg cgcgcttaaa acgatacgac ggatcaggta ttacgatgga gaagacaaag
     1741 aaaggtctct taaaacaatg ggatcgcaaa agccacgaat tatttatcag tattttgaaa
     1801 gaattggaag aacacgagaa ttggtatgcg gaggtggagt aattggctga catcaaagga
     1861 cttgatattt ttgggaacaa caaaggactc gccaaaataa aaaaagaagc agaagcaaag
     1921 aaaaagcagc aagcaattac accatctgaa aataagcaat tagaagtgca ccaagttcaa
     1981 gaaataaaaa cttcctccaa tgttgaaaga cagagtagag gaagaacaaa aaaagttggc
     2041 gcaccaatca gaaagtttga taaggtttat tccaccaagc atcctttaaa actgtcgact
     2101 ttgttaaatg cgacttccag agttttagta gaaaagtatg agactaatat gacaagagat
     2161 gaattgctta gaaaagcttt agatgagtat attaaacaga atcttacgca agaagataag
     2221 caagatttgt ttaatgacgt ggtgaaagaa ctagacttgt tcagagaaaa atatccaatt
     2281 gttccagaat tagatgaccg tggagaaata ataagaacta ccagtcagat aaaacaagat
     2341 accgaagact cactgcgcaa agggtgggga atgaataata aagttagata gaaccccctt
     2401 tttatagcat aaaaaagatt tagaaggata tatgatccta ttgcatgaga agtacaataa
     2461 aattaataag aagtaacatt aacgaaaaaa cgtggaaatt attcagtgaa tacaaagaaa
     2521 ttaaatcttg aaaagtaatg gagtatggct tggaaaagtt caaaaaatat caagacaagc
     2581 ttctcaaaaa aagggatttt gatatccaat aaaaagagtg aatcattgta ctgccctcca
     2641 aaaaagtagt accaaaaatc taaatttttt ggaggatttt acatgactca ctcttttttt
     2701 atcaaagaaa gtattttcca agcagttttt tcatccaaaa ataaatctgt aaaaaaatta
     2761 ccattcaagg cgcctaaaca actctccgct ttgaacttac ttttacaaat accaattcgt
     2821 tttggaatat cgaaaatttc ttttaaagaa agaccaaaag tcttatcctc agatgctttg
     2881 atgaattcac cattgatatt gaagggtctt ccgtaaagaa atccaacagt atccgtcatg
     2941 cttggaaata ggattttacg atgcttgttc cagggttcga cagaatcaat agaggataag
     3001 gtgccaattc cagaaattaa catatctgca tgcgaagcgg ttgctaaggt attagctaaa
     3061 gaaggttcta gagccattag ttcacgtgca gtttcattga tgacatataa aggaatactg
     3121 agagtcaaat aagaagactc ataacgttca gcagcttttt gaactaatcg tgtggagcca
     3181 gctaaggaat tatattttcc gatttcgcca atgaattgag taaaaattaa ttcttcacga
     3241 gcacccattt taaactgatc aatcactttg taaacggtat ctccccaaga tagtgcaacc
     3301 acattggctt tcttaattaa ttcttgaatg taatcggcag caaattttga aaataataaa
     3361 tcgtgttgtg ataagtcttc actacttttt aatatataga tattcgtttt aaagtaattt
     3421 ttgaagattt cttctagctc ataattccta gcatacggag aattaattga aatactaact
     3481 aattgttttt cacgtgcatc ttcaatatat tttaaagctt tatatctggt gatcccatat
     3541 tttttagaaa tttcactaat attgatatcc tcgagataat agtcttcaca aatttttaca
     3601 aggagtgatt catagtcaga gaattccaga tgcatagtat agctcctaac tttttcaaat
     3661 agatattttt tctcattata ccaaaaaaat tgaatttagt ctttttttaa atttttaaaa
     3721 ataagcatca tttttttaaa atttaaaatg aaagggtttt tttattttaa aaaaagtgtt
     3781 aaagtggttt tgtaattaaa aaatgtaaaa aggagtgaag gtatgcttga tgaagtttta
     3841 gaaatccata aaaaaaatgt aggtgtttta cggatggaaa caacagaagc cgtgcaagat
     3901 gatcaagatt tagcaaaaat gtacacccca ggtgtagcag aactaagttt aatgattgag
     3961 aaaaatcatg ctttggcaag agaattaaca attagtggta aattagtggc cgttattaca
     4021 gacgggtcgg cggtattagg tcttggaaat gtaggaaacc aagctggatt accaatcgtc
     4081 gaagggaaag cgctgcttta taaaaattta gcaggtgtca atgcgattcc attagccatc
     4141 gaacaaaaat cagtagacga gattgtacaa acaattgtca atttgcaaaa tagttttgca
     4201 gggatccatt tagaagatat tgcagcaccg aagtgttttg agattgaaga aaaattacaa
     4261 gaaaaacttt ctattcctgt gtatcatgac gatcaagaag gaacagcgat agttgtttta
     4321 gctggattga tcaatgctgc taagatccaa aggaaaacat taactgaact tcgagttttg
     4381 atcaacggca tgggagcatc tggggttgca acagctagac tattaatcgc tgctggtatc
     4441 aaaaatctaa cattggtgga taaacaaggg agattaaaga agcaagatag tacattaaat
     4501 aaatatcaaa gagagttgtt ggatcatata catgatacaa ataataatgc aagcgttttg
     4561 gaagatgcaa tcgttggaca agatgttttt atcggtcttt caataggaaa tgttttaacg
     4621 cctgaaatga ttggaaaaat gaatgataat ccgattattt ttgctttagc caatccagta
     4681 ccagagatca cacccgattt agcaaaagaa tctggtgcaa agctaatcgc tacaggttct
     4741 tcgaaatatc ctaaccaagt aaacaatatt ttagtatttc ccggattgtt caagggacta
     4801 ttactatcga aagcgaataa agtcgatctt tcattacaac ttgaagtagc caaacaatta
     4861 gctgctaagg tcgcagagcc tacggtagaa aaattcatcc ctaatgtttt tgatgaagga
     4921 gtcgcaaacg tcgtttcaca ggcagttttg gattatttac aaaaactaaa agaaaacgag
     4981 gataatatac atgctgaaaa cattaaatag aattaaaatt ggttttattc ctttatctct
     5041 ttatatcatt ttggtactcg cgtatcttgc aatgacgata agcaatatcg ttccagaaaa
     5101 tgatatgtta gtttctttgg gtattatgac tgtttttgct tatttattag aagaaattgg
     5161 taaaactatt cctattttaa aagatttagg tggtaaagta ttagttgtta catttttgcc
     5221 tgcatttcta gtctataaac attggctacc tcaaagtact gtaactgtgg tttctgattt
     5281 tatgaataat aacaatttct tgatgttctt tatcacacta ctagttgtcg gcagtattgc
     5341 ttcaatgaat cggcaaacat tggttaaagc aacttcacgt attatcatta ttttaatttt
     5401 atgcgatgtg attggttctt tgataggaac aggtgtggga atactccttg ggctatctgc
     5461 ttggaatgcg tatttcttta tcgttgcgcc gattatggct ggtggtgttg gggaaggagc
     5521 gatgccatta tcgattggtt atgcagcaat attatctggt atgggacaaa atgatatctt
     5581 tgggaagatt ctaccgtgtg tgatgttggg aagtttagtc gcagttctac tgtctggtat
     5641 gctcaaaaaa attggagaga aatacccaca attaaatggt cagggtaatt tgatcgatgg
     5701 ggatgatgaa catttagaga acttgaaggg ttcagctaaa ccagtcaata ttgaaaaaac
     5761 attgttagca ggcgtggtag caataacgat ttattttgtt gctgtctatg ttgagcactt
     5821 attaaaatca ctccttggct tttctattcc ttcacctgtg atcttattag tctctcttat
     5881 gggggctaaa atgcttggtt ttatccctaa aaatatcgaa gaaggtggaa attcgttgta
     5941 tggattcact gttcgtggga tcacacctcc cttacttttt ggcgtagggg ttgcaaaaac
     6001 agattggtta gagctgatcg aagtatttaa aaatcctgca acgatagtaa caatcattgt
     6061 gacggtagta gctattgtcg ctacagcttt tatcgccgca aaatttttaa aaatgtaccc
     6121 agttgatact gccatcgcag tttcatgttg tagcggacaa ggcggtacag gtgctttagc
     6181 tatcttagct gcgggtgatc gcatggaatt aatgccattt gcacaagttg ctgttcgact
     6241 agggggtgct atgaccgtaa cgttcgctat ctttttaatg ggtctgctat cataagaaca
     6301 taaagaaagg acgtgaccat gtgtttgatc aaacagatat ccaacgatta tttttaaaaa
     6361 ataactatat caaagaccaa tgggaaaatc ttttgcttga ttcaggaata caaaagaagc
     6421 aaatcgaaga ttttcaacat ttagatcaaa cattgggtat ataccataaa gaaaagttag
     6481 taggaacagt atcttatcaa aacaacgtga ttaaatatat cgccgtgtct gaaaaatata
     6541 aagatggtgg acatacattt aatgtactag tctctgcaat aattcaggca atgagtcaac
     6601 aagagatttt tcattatatt gttttcacga aaccgcgcta tcaaaaaagt tttaactttt
     6661 taggttttga aacaatcgta tctaccccaa aaatagctgt attagaaatg ggagatagtt
     6721 ctataaaaca gtacctagag aggattccta aagtgaatgg tgaaaaaaaa gcagcaattg
     6781 tcatgaatgc gaacccattt acagaagggc attattattt agtaaaacaa gctgccaata
     6841 acaacgattg ggtatatgta tttgtactcg aaaaagaaca aaatcttttt tcgacgaagg
     6901 accgactcaa attagttcaa caaggattac aaaaactgaa aaatgtcatt gttctatcag
     6961 gtggcgagta tattattagc cctgcaacgt ttcccaccta ttttttacga gaagatgatg
     7021 aaatagcaaa ggagcaaatg gtcgtagatg ctactttatt caaagaaaga attgccactt
     7081 atttagatat ctctgctaga tatgtgggag aagaaccact ttccccaatg actcgttcat
     7141 ataacgacat actggaaacg attttacccc ctgaaataaa agttcatgtc cttgcaagga
     7201 agaaaacgga acaacaccaa gttatttctg cttctcaagt tcgtaaagcg tatcttgctg
     7261 ggcaattgga aaaaataaaa tatatggttc cagaaacaac ctatcaatac ttaaaaaata
     7321 aaagggagcg ttaagtaatg gaaataaata aatcagcgat tgctggaaca ttagagtcaa
     7381 gtgatgtcca aatcatgatt tcaccgaata cagaaggaat agaaatcgat ttagacagtc
     7441 aagtgattga gcaatttgga aaacaaatca aaaaagttat ctatgacaca ctagcagttt
     7501 atcagattaa aaacgtaaaa ctcaaggtag tagataaagg ggcattagat tgcgttattg
     7561 ttgcacgatt aaaaacagcg atccatcgtt ccttaggtat ccaacaccaa gaaacaccgt
     7621 gggaggttta attagatgat gaaagaacgt ttgagaagaa caatgatgtt tgttccagga
     7681 aataatccag cgatgatcaa agatgcaggt atttatggtg ctgactcgat tatgttcgac
     7741 ctggaagatg cagtgtctat ggcagaaaaa gacagcgcaa gaatgcttgt ggctgaggca
     7801 ttgaaaacag tggattatgg tgacacagaa ttaattgttc gaataaatga cctgcactca
     7861 gaatttggac gtgcagacat ttttgcaatg gttaaagccg gcgcgcatgt attacgttta
     7921 cctaaaacag aaacctctga agatataaca gaagtagaaa aagtgattat agaagcagaa
     7981 aactatcata atgttccgat agggcgaaca ttgatgatgg cagccattga aggtgcttct
     8041 ggtgtattga acgcacctgc gatcgccaaa gcatctgatc gattgattgg aatcgccatt
     8101 ggggcagaag actatgtgac aaatatgaaa accaaacgat atccagatgg aaacgaattg
     8161 ttttttgctc gcagtatgat cgttcatgct gcacgtgcag caggcatttc cgcatttgac
     8221 actgtttact ctgatgtgga tgatatggaa ggatttttaa acgaagtacg tttaatccat
     8281 caattaggtt ttgatggaaa atcttgtatc aatccacggc aaattccagt agtcaatact
     8341 gtttatcagc cgtcaatcaa agaaatcaaa cgatcactag acatcatctc ggcaatcgaa
     8401 gaagcaaaag aaaaaggttc tggcgtaatt tctttaaatg gaaaaatgat cgataaacca
     8461 attgtagaac gtgcacaacg gacattgatg ttagcaaaag catcacgatt gattgatgag
     8521 gagggaaata tccatgcata ataaacaaag acgtagcatt ccatcaagtg ttttaacaaa
     8581 gtataaaaaa gaattccaaa gtgtttcatt atcgccacaa acgattcaac gttgtgcacc
     8641 aaaagtaacg actgtaacta atcagacaaa taaattgatg cactcgatca aagaagctat
     8701 agaaaaatcg ggcttaaaag atggaatgac gatttcattt catcatcatt tgagagaagg
     8761 tgattttgtt tttaaccaag tgatggccgt gatccgtgaa atgggcatca aagaattgac
     8821 cttagcacca agttctttaa cgaacactat gaatgagatg gttattgaat gcatcaagga
     8881 cggtacagta actcatatca caagcagtgg gatgcgcggc agcttaggtg aatttatttc
     8941 tcatggagga atggacaatc ctgtgatttt acgttctcat ggtaatcgtc cacgtgcaat
     9001 tgaaagtggt gaattgaaaa tcgatgttgc atttctagga gtaccaacat ctgatgatta
     9061 tggaaatgct aatgcgactg ttggaaaatc cgtttttggt tcattagggt atgcaaaagt
     9121 agatgcacaa tatgcagata aagtcattct tctaactgat catttagttc cttaccctaa
     9181 tacgcccatt agtattccgc aaacacaagt cgactatgtc gtggaagtag aagcaattgg
     9241 tgatccgaat aaaatcggtg ctggtgctac acggtttact aaaaacccaa aagaattaaa
     9301 aattgctgaa atggtcaaag atgtcattgt tcactcacct tatttcaaag atggcttttc
     9361 ttttcaaaca ggaacgggtg gtgcagcttt agcagtaacg cgttacttag ctagtgaaat
     9421 ggaagaaaaa cagatcaaag cttcttttgc attaggtggc atcactaaac caacggttga
     9481 tttattggaa aaaggtcttg ttgaaaaagt gttagatgtc caagactttg acaaaggagc
     9541 agctgaagga atggcgcggc ttgagaacca acaggagatc gatgcttcct tttatgctga
     9601 tccaagtaat aaaggagcgg ttgttaacca attagatgtc gctattttat ctgcattaga
     9661 aattgacaca aacttcaatg taaatgttat gactggttca gatggtgtgc taagaggtgc
     9721 gattggtgga catcaagatg cagcaacggc taaacttact atcatctcag cgccgttagt
     9781 acgtggccga atacctacag tagtaaatga tgttactaca ttgatcacac cagggaaaag
     9841 tatcgatgta ttagtcacag aagtaggggt tgctatcaat ccccaacgta aagatctaat
     9901 agctattttt gaaagaattc cacaaattcc tgtttttaca atagaagaat tacagcaaaa
     9961 agcagaaaaa atagtaggca tcccggaacc tttgcaattc acagatcgga tagttgccta
    10021 tgtagaatac cgagatggtt caattttaga tgtcgttcgt caggtgaaag aatttgaaga
    10081 agagagatca tgatgtttga tactggagaa attcaagaaa tcgaggatat tttgatccat
    10141 aaagaacaac gtgctaactt gcaaagcaac ctgctttcca agtattcaaa tagttcattg
    10201 atggtcttta cattgaatat tcctgggccg atcaaaaata atccaatgat tgagcaggta
    10261 tttcgtcagg gacagcagtc aattcagcaa atgtttttaa aaaaaaaggt ggaaatgacg
    10321 tacacaaaag aaatcaattt gccaagtgga ttggactggt acgtaatagt aacagaagca
    10381 cctttgaatc tcaaaaaatg gttggtcacg ttagaagagc aagacgcatt aggtagactt
    10441 tttgatttag atgtcttgtt caaaaaagga gagcagataa caattgtcag tcgccaagct
    10501 cttaatcatg gggaacgtcc ttgttttatt tgtgggaaat cagcaaaggt atgtgcacgt
    10561 aatcgtacac atactgtgaa agaaatgcaa aagaaaatcg aacaaatagt agccgattcc
    10621 ttaaaaggag cattgaaaca tgcatgataa tattcaaaaa atagcacaag aagcacaatt
    10681 agcgatgctt tatgaagtta catgtatcaa taaaccagga ttggttgatc cagtagaccc
    10741 cgggtctcat caagatatgg acatttttac ttttctacaa agtagtgtag ttttgactcc
    10801 ctattttgaa gaatttatcc aaacaggatt ggaccgacaa catcaaccaa ttgaagaaac
    10861 ctttatggcg attcgacaga ttggtttaga ggctgaaact gcgatgttct cagtgacaaa
    10921 tggtataaat acacataaag gtgctatctt ttcattgggg atttttcttg ctatttgtgg
    10981 ccggttaact atttggaaag ttccttgtaa aaaaagtttc tttcaaaaag aaattcaaaa
    11041 aatgacgaaa aatctactta acgattttgg gaagattgat caaacaaaac caaaagcatg
    11101 gacttgggga gaatatctat ttgttaacca tggattgaca gggatacgag gggaagccaa
    11161 aagaggttat ccttgtgttt ttgaccgagg attgccttat tatcaagcat atcaagggtc
    11221 gcaacaagat aagatgatag acactttact ttttctatca atggaagtag aagataccaa
    11281 tttgatcaaa cgaagtaata atagacatgt ttttgaagag tatcaattac ttattcgtcc
    11341 ttattttgaa ttagggggtg taaaaaccat acaaggtaaa aaatatttgg attacctaaa
    11401 tcagcaattt aaaaagaaaa attggagtat tggtggaagt gcagacttat tgatccttac
    11461 aatcttttta gataaaatca tggacaaagg atggttatgt tgatactata atacattatt
    11521 ttgtattagt aagctaataa ctaaaaaatg gttcaataca aatattttat aaaaaatggt
    11581 agtgcctatc ctttcagatt gtagacaaag aaaaatttct ttgtctacaa tttttttgtt
    11641 aatgacttca gtcagtttcg tgcgcaaagc gtgcgaaaca agaaaaccat gcgtttctac
    11701 acgtggaaga actagcatat cgaaaggagt tttttcatgt caaatgacga taaaagttta
    11761 gcccatactc gttggaattt taagtatcat attgtattta caccaaaata tcgacgaaaa
    11821 gtaatctata ggcaattacg aaaagatatt ggaaaaatac ttaggaagtt gtgcaaaata
    11881 aaaaacgtgg aaattataga agcacatgca acgccagatc atattcacat gcttgttcaa
    11941 gtaccatcaa aaataagtat atccagtttt atagttatct gaaagatcgt agtgctgtgc
    12001 taatccacga gcagcacgta aatcacaaat atagatacgg aaataaatca ttttggtcgg
    12061 aggaatatta tgtgagtaca gtcgaattga atcaaaagac aattgaaaaa tacatccgtg
    12121 aacaagaagc tgatgatcgc atttgagata gtataagtaa aagagagtag atgttgcaat
    12181 aacaaaaggg taaaaatgga ctacctccag taaaataccg aaaaatagtt taacttttgg
    12241 agataagtcc atttaagggt ctatagaatg acaatttttt taggggattt tactattatt
    12301 acttcaagac ccgcttggat tcgcaaagtg agtgaactct taaaactacc tgatagtagg
    12361 ttgttttaag agttatctga aagtaaaaat tgcatttata tttttgataa atacgtaaat
    12421 ttatatatac gtatttatca aagttttagg ccaaaggaat attgtgtaaa tacactagga
    12481 ttgaatgaat agaccagaag aaattgcgtc caagaacaaa agaagcatga tagtgctcta
    12541 tataaattgg gcattcaaga atctgaaatt taaaggtaga attttagtta gacttttaga
    12601 ttttaaacac aaaaataacc atacaagcaa caagaagcaa agtgctatat tataaattgt
    12661 ccgtccgttt acttacataa gatcattggg gctattttta aaagagaata atagataata
    12721 aatgaaaaaa aagtcaagtc cacaatcctg tgtaaaatac tatacaatgc tctcactatt
    12781 ttttacttgg caaatttaat atattttctt gatgaagaaa gccactcatc gtgttgatca
    12841 ataaggactg ctccaattaa acgattggct gaaacgcagt ttggaaagat acgaataatt
    12901 ttttctcttc tgcg
//
LOCUS       DACEOE010000069        12505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEOE010000069 DACEOE010000000
VERSION     DACEOE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.071"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(37..834)
                     /locus_tag="IW774_001601"
     CDS             complement(37..834)
                     /locus_tag="IW774_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1153949.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(831..2015)
                     /locus_tag="IW774_001602"
     CDS             complement(831..2015)
                     /locus_tag="IW774_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1153950.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2040..3287)
                     /locus_tag="IW774_001603"
     CDS             complement(2040..3287)
                     /locus_tag="IW774_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1153951.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3502..5247
                     /gene="dnaX"
                     /locus_tag="IW774_001604"
     CDS             3502..5247
                     /gene="dnaX"
                     /locus_tag="IW774_001604"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1153952.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5267..5581
                     /locus_tag="IW774_001605"
     CDS             5267..5581
                     /locus_tag="IW774_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1153953.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5606..6202
                     /gene="recR"
                     /locus_tag="IW774_001606"
     CDS             5606..6202
                     /gene="recR"
                     /locus_tag="IW774_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1153954.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6266..6910
                     /locus_tag="IW774_001607"
     CDS             6266..6910
                     /locus_tag="IW774_001607"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1153955.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            6925..7254
                     /locus_tag="IW774_001608"
     CDS             6925..7254
                     /locus_tag="IW774_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153956.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7268..8206
                     /gene="holB"
                     /locus_tag="IW774_001609"
     CDS             7268..8206
                     /gene="holB"
                     /locus_tag="IW774_001609"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1153957.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8242..9066
                     /locus_tag="IW774_001610"
     CDS             8242..9066
                     /locus_tag="IW774_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1153958.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9059..9406
                     /locus_tag="IW774_001611"
     CDS             9059..9406
                     /locus_tag="IW774_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1153959.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9475..10347
                     /gene="rsmI"
                     /locus_tag="IW774_001612"
     CDS             9475..10347
                     /gene="rsmI"
                     /locus_tag="IW774_001612"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1153960.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10456..11577
                     /gene="dinB"
                     /locus_tag="IW774_001613"
     CDS             10456..11577
                     /gene="dinB"
                     /locus_tag="IW774_001613"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1153961.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11631..12233)
                     /gene="nrdG"
                     /locus_tag="IW774_001614"
     CDS             complement(11631..12233)
                     /gene="nrdG"
                     /locus_tag="IW774_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1153962.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
BASE COUNT         3981 a         2345 c         2678 g         3501 t
ORIGIN      
        1 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
       61 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
      121 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
      181 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
      241 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
      301 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
      361 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
      421 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
      481 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
      541 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
      601 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
      661 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
      721 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
      781 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aattttttca tcatatcaag
      841 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
      901 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
      961 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
     1021 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
     1081 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
     1141 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
     1201 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
     1261 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
     1321 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
     1381 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
     1441 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
     1501 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
     1561 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
     1621 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
     1681 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
     1741 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
     1801 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
     1861 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
     1921 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
     1981 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
     2041 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
     2101 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
     2161 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
     2221 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
     2281 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
     2341 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
     2401 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
     2461 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
     2521 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
     2581 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
     2641 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
     2701 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
     2761 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
     2821 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
     2881 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
     2941 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
     3001 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
     3061 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
     3121 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
     3181 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
     3241 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
     3301 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
     3361 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
     3421 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
     3481 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
     3541 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
     3601 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
     3661 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
     3721 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
     3781 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
     3841 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
     3901 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
     3961 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
     4021 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
     4081 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
     4141 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
     4201 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
     4261 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
     4321 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
     4381 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
     4441 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
     4501 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
     4561 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
     4621 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
     4681 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
     4741 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
     4801 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
     4861 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
     4921 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
     4981 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
     5041 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
     5101 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
     5161 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
     5221 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
     5281 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
     5341 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
     5401 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
     5461 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
     5521 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
     5581 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
     5641 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
     5701 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
     5761 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
     5821 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
     5881 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
     5941 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
     6001 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
     6061 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
     6121 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
     6181 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
     6241 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
     6301 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
     6361 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
     6421 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
     6481 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
     6541 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
     6601 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
     6661 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
     6721 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
     6781 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
     6841 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
     6901 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
     6961 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
     7021 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
     7081 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
     7141 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
     7201 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
     7261 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
     7321 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
     7381 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
     7441 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
     7501 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
     7561 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
     7621 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
     7681 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
     7741 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
     7801 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
     7861 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
     7921 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
     7981 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
     8041 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
     8101 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
     8161 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
     8221 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
     8281 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
     8341 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
     8401 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
     8461 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
     8521 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
     8581 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
     8641 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
     8701 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
     8761 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
     8821 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
     8881 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
     8941 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
     9001 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
     9061 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
     9121 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
     9181 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
     9241 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
     9301 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
     9361 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
     9421 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
     9481 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
     9541 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
     9601 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
     9661 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
     9721 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
     9781 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
     9841 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
     9901 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
     9961 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    10021 aatttcttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    10081 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    10141 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    10201 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    10261 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    10321 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    10381 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    10441 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    10501 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    10561 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    10621 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    10681 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    10741 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    10801 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    10861 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    10921 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    10981 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    11041 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    11101 aaagacctat atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    11161 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    11221 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    11281 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    11341 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    11401 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    11461 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    11521 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    11581 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    11641 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    11701 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    11761 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    11821 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggtatagcct
    11881 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    11941 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    12001 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    12061 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    12121 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    12181 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    12241 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    12301 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    12361 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    12421 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    12481 aaaatatgag gaactatttt tgttt
//
LOCUS       DACEOE010000070        12463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEOE010000070 DACEOE010000000
VERSION     DACEOE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.072"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            255..530
                     /locus_tag="IW774_001615"
     CDS             255..530
                     /locus_tag="IW774_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153963.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            543..2621
                     /locus_tag="IW774_001616"
     CDS             543..2621
                     /locus_tag="IW774_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1153964.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2781..4667
                     /locus_tag="IW774_001617"
     CDS             2781..4667
                     /locus_tag="IW774_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1153965.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IW774_001618"
     CDS             4679..5626
                     /locus_tag="IW774_001618"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1153966.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IW774_001619"
     CDS             5646..6173
                     /locus_tag="IW774_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1153967.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IW774_001620"
     CDS             complement(6236..6889)
                     /locus_tag="IW774_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1153968.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IW774_001621"
     CDS             7021..7863
                     /locus_tag="IW774_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1153969.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IW774_001622"
     CDS             8021..8917
                     /locus_tag="IW774_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1153970.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IW774_001623"
     CDS             8920..9633
                     /locus_tag="IW774_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1153971.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IW774_001624"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IW774_001624"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1153972.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IW774_001625"
     CDS             10168..10395
                     /locus_tag="IW774_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1153973.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IW774_001626"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IW774_001626"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1153974.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IW774_001627"
     CDS             complement(11181..11723)
                     /locus_tag="IW774_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1153975.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IW774_001628"
     CDS             complement(11960..12358)
                     /locus_tag="IW774_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1153976.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         4338 a         2127 c         2490 g         3508 t
ORIGIN      
        1 tttgtcgcag ccccattttt ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag
       61 taaacatcct tttgtcagaa acttttatct ttcttaagtt ttacttgata tctgatacta
      121 tttggaatag aatgaggcgt tgaaaaattt atggaaattt ctaactagca ataaacgatg
      181 aaacaatgaa atatttttca tccaaaaaga actttgctat aatgatggaa gtttgtgtat
      241 aggagatctg gaaaatgaga gagagttttg aacaacaaaa aaaactgttg catgatcgct
      301 atggcgccct ttcgatggat gaccgccgtc aaatactttg taagttgcgt aaaagaaata
      361 tattgatgta tcgtcagttg gaacgtctga aacatgatct gcttcgtttg gagtcaaaac
      421 gtgtacagtg tgaattagag gggaaccaaa cacaggttga agtagtcgaa accaaaattt
      481 taaagaaaaa agaacaattt ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga
      541 cgatggaatt tgctgagtta gttattgtat ttgcagtaac gatcacctta tcgaacatag
      601 ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt ggagtggtat
      661 taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc ttagttatga
      721 ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg attctcaaaa
      781 atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt gtcggggtag
      841 gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt gcttttggag
      901 cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga gtgaatatcc
      961 ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca tctggtgtta
     1021 ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg
     1081 ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg cttgtctggt
     1141 tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc acagggtatc
     1201 tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca tttgatgtat
     1261 ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt aggaaaatct
     1321 ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact attgctttta
     1381 cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg ttctcgccag
     1441 tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta ttaatctctg
     1501 tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc aaagatggca
     1561 gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg
     1621 gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat atgatgtttt
     1681 accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact ttggtcatag
     1741 ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg
     1801 ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc
     1861 gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa gaacggatac
     1921 gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa gaaatacaag
     1981 cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg ggcaagcttt
     2041 cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat tctatcacaa
     2101 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
     2161 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
     2221 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
     2281 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
     2341 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
     2401 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
     2461 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
     2521 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
     2581 cgtttcgagc aaagtcaaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcattttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat cat
//
LOCUS       DACEOE010000071        12424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEOE010000071 DACEOE010000000
VERSION     DACEOE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.073"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            125..712
                     /locus_tag="IW774_001629"
     CDS             125..712
                     /locus_tag="IW774_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153977.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(1191..3200)
                     /gene="metG"
                     /locus_tag="IW774_001630"
     CDS             complement(1191..3200)
                     /gene="metG"
                     /locus_tag="IW774_001630"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1153978.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            3468..3746
                     /locus_tag="IW774_001631"
     CDS             3468..3746
                     /locus_tag="IW774_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1153979.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            3843..4664
                     /locus_tag="IW774_001632"
     CDS             3843..4664
                     /locus_tag="IW774_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1153980.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4949..5959)
                     /locus_tag="IW774_001633"
     CDS             complement(4949..5959)
                     /locus_tag="IW774_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1153981.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5971..6924)
                     /locus_tag="IW774_001634"
     CDS             complement(5971..6924)
                     /locus_tag="IW774_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1153982.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6914..7984)
                     /locus_tag="IW774_001635"
     CDS             complement(6914..7984)
                     /locus_tag="IW774_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1153983.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7990..9033)
                     /locus_tag="IW774_001636"
     CDS             complement(7990..9033)
                     /locus_tag="IW774_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1153984.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(9034..9975)
                     /locus_tag="IW774_001637"
     CDS             complement(9034..9975)
                     /locus_tag="IW774_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1153985.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            10059..10406
                     /locus_tag="IW774_001638"
     CDS             10059..10406
                     /locus_tag="IW774_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1153986.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(10476..12137)
                     /locus_tag="IW774_001639"
     CDS             complement(10476..12137)
                     /locus_tag="IW774_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1153987.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3523 a         2486 c         2245 g         4170 t
ORIGIN      
        1 gaatatttta atttaccatt cttttttctc cactttcagt gaaaaactcc ctcttaagac
       61 ggatgtattt cttttttatt tttcgtatac taaacctacc ttttccacga aggaggaaca
      121 gtttatggat tttaagcgtt cttatccgaa attagcagat tatttatcca accgggtcga
      181 tttttcccgt aaagaacaac agcagtccca ttggcattgg ttcacattaa ttttatttgg
      241 attaatgact gttgcaacgt tatcacaaaa actaatattg ctgactttgt tcatcgcaat
      301 cgttgcattg atcaaaggtc ctgtgatgat cttgtggggg atgttgtatt cctttttggt
      361 tgggcttttt ccgcctatcg gtattttgct ttcagcgatt ttctttttat tgaatatcgg
      421 aacatttaca aagaactggc ggatgacctt agtcgggatc tatttttatt tctatccttt
      481 tggagtgatg gcacttagtg aagtcatgca ttgggataat cattggttta tagctggctc
      541 cttgctttta gggttgatct tgttacacgt gatgttgaca aagctctatc aacattatgg
      601 tatcggacgt acgattttct ggtatgtctt ttcgatccca tttgcactgc ttactgctct
      661 cttaccaagc cgtttgaaga caaaaataaa aggttaccat aaaatcaagt gataggattt
      721 ttacaaaata aagaaaacaa tcatcgaagt caaagccgat gattgttttt ttatctatat
      781 tttcaatcac acatataaga gcacaaacta attgaacata aagtgataga acttataatt
      841 aacacatcaa cgctactgtt agatataaat atagatgtaa taaagtcatt gcattcaaac
      901 tttatgaatg taaggacaat aaaaaagagt accagatgaa tcaattaaaa tctgcccgac
      961 cctttatcat tatgcttttt ttctgttttt acgattccta ttagtattat tctaaattaa
     1021 caaaaatcag cgaagctcgt acttcttttt ctaggattta ttttggatag ttctctgttt
     1081 ctgtattata tgattggctt caagtaataa gaaggaattt acgaaaattg cttctcaaat
     1141 ttcggaaaaa gggggtatga cacaactagt gtcatacccc ctttgaattt ctatgcgatt
     1201 tctgagccat ttggcatgga ttttggtgct tcgattactt gtaaagagcc atctggtgct
     1261 tctgcagaaa ggatcattcc ttgactgatt tgtccacgca tctttcgtgg tttcagattt
     1321 gctacaatga cgactttttt cccgatcaat tcgcttggat caggataaaa ttcagcgatt
     1381 cctgacaaga tttgacggtc ttggctgtct ccagcatcta gtcggaattg cagcaactta
     1441 tctgcccctt ctactttttg acagttgatg acttctgcta cttttagttc tactttttca
     1501 aatacatcaa atttgatttg tttttctttc gttgaaacaa gttctgtttc ttctggatcc
     1561 catttgactg tatcttcgtt tgtttgagta ccttcagaca ttttttcttg aataaaagca
     1621 acttcttctt ccatatctaa acgtgggaag attggtgtac cttttgcaac tacttttgtt
     1681 cctgaaggaa attcgccaaa gtgtaatcct tcaagattca ttgtttcaga gtccaatcct
     1741 agttgattga agatcttctc tggcgtttct gtcatgattg gctgaagtaa tatcgctgcg
     1801 atacgcaaag attcagctaa atggatcatg acactattca attcagcttt cttttcttcg
     1861 tcttttgcta atacccatgg ttctgtttca tcaatgtatt tgtttgcacg agaaatcagc
     1921 acccagattt cagccaatgc tgtattgaat tccatttttt ccattgcatc atggtaacgt
     1981 ccgacaacgt tagcagccgt cgtagacagt tcactatcaa atggagtgac aagtgaggca
     2041 tagtcaggaa cgagtccatc acaatatttg ttgatcatag caatcgttcg attcaataag
     2101 ttccctagat catttgccag atcataattg acacgggaaa caaaatcttc tggcgtaaag
     2161 acaccatctg acccaaacgg aacagcacga agcaaataat aacggagtgc atctaaacca
     2221 taacgttcca caagcatttc aggataaacg acatttcctt ttgatttaga catttttccg
     2281 tctttcatca atagccagcc atgcccaaat acttttttag gtagaggcaa atcaagggcc
     2341 atcagcatga ttggccaata gatcgtatga aagcgaacga tttctttacc gaccatctga
     2401 acatctgcag gccagtattt ttcgaataga ctttcatctt cggagccata tcctagtgca
     2461 gtaatataat tggataatgc atcgatccat acatacacca catgtttcgg atcactcttg
     2521 actggtactc cccaagtaaa agttgttcgt gaaactgcaa gatcctctaa tcctggtttg
     2581 ataaagttgt tgatcatttc gtttttacga gattctggct ggatgaattc gggatgttct
     2641 tcatagtatt ccaatagacg gtctgcgtat ttgctcatgc ggaagaagta tgattcttct
     2701 tttaccaatt ctacttcgtg tccacttggc gctttaccac cgatcacgtt accttcgtca
     2761 tctttgtaca cttctgcaag ttgtgtttct gtaaagaatt cttcgtcaga aacagagtac
     2821 cagccttcat attcacctaa gtaaatatct ccctgttcta ataaacgatc aaatattttt
     2881 tgaacagctg ctttatggta atcatctgtc gtgcgaatga atttatcata actgatatca
     2941 agcgttttcc ataatttctt gacatcagcc gccattttat ctacatattc ttgcggtttt
     3001 acacctagtt cttctgcttt tgtttcgatt ttttgaccat gttcatccac tccagtgaga
     3061 taaaacacat caaagcccat caaccgttta taacgagcaa ttgcgtcaca tgcaatcgta
     3121 gtatacgagt tgccgatatg aagtttgccg cttggataat agattggcgt agtgatataa
     3181 aaagtttcct tttctgccac ggggctcttc ctcctttatc gcatgatgcg aattcgtttc
     3241 cttgctagta taccataaaa aaagcatgct gttagctttt tttcaaaatg aaaaagactg
     3301 gataaacgac ttctatctac gttcaatcta tcctttaaaa ggatgaatag gttgaataaa
     3361 tgcgggtttt tgttttgttt catatctact aaaaaacggc tttttatttc tcccctcaca
     3421 cgtggtataa taaagactat ctatcttagt aagggactgg aacaatcatg cagaagaaac
     3481 atcttttttt tacacttagt attgcttttc tttcattagc acatttaata ttcagctatt
     3541 tttatattcg tatgtatggt tattttaacc tccatgggca tttgaacagt tttatgacag
     3601 cagcttggat tttgcgtttt atcattgacg tatatatcgt tatttgtggc ttctttgcca
     3661 ttcgtgaaga gcggtataaa gtattgccgt tttatctgct tttcttttta ttcaatctca
     3721 tacttccatt tatttttcat atttaaatga taacctagat ttttcaagta aatccttggt
     3781 tttttttcag agtctgctac aataaggtaa aaagtacttt tactgtcaat ggaggtggtc
     3841 agatgaaccg ttttgcttct aaagaagaag aaaaattata ttatgaacat aaagcaagtg
     3901 aagaagaatt tcttaactgg tatcaaaaac aggaacgtcc agaatatgac aagccctctt
     3961 tgactgtcga tatcgtactg atgtgctata acaaagaaga agatcagctg aaagttttgt
     4021 tgatccaaag aaaagggcat ccttatcgaa attcatgggc attgcctggt ggctttgtcc
     4081 aaaaggatga gtcaactgga gaaagcgtct taagagaaac aaaggaagaa actggtgtgg
     4141 ttatttcaaa agaaaatatt gagcaactgc atacattcag tacgcctaat cgagatccgc
     4201 gaggctgggt cgtcacagtc agctacttag cctttattgg agaagaaccg ctaatcgccg
     4261 gagatgatgc caaggaagta cgctggttca cactagaacg tcatagtaac aagatccatt
     4321 tatctagcgg agaagtggct atctcgcttg atttagtcac tggagagtct tctggcaaag
     4381 atactttggc ttttgaccat agtcaaatca tcttgaaagc attcaaccgt gtagtcaata
     4441 aaatggaaca cgagccgcaa gttctacaag ttttaggtaa agatttcacg attacggaag
     4501 ctcgaaaagt atttgctaaa ttcttaggga tcgattataa aacgatcgat cattccaatt
     4561 ttaagaaagc tatgctccaa tatttcgatg agatcggtga acgtcctgta ggaatcggcc
     4621 gtccttcaaa aatctatcag ctcaagccgg ggcaccatga ataaggttgt gagcaaaaga
     4681 ttcagaccta atcattcaag aaaaaacgca aaaacaactc tttgtgagtt acgttttttg
     4741 tttcagaatt ttgatgtatt cgtaaacttt ctgaataaaa aattaacaat agcagaaaaa
     4801 gctcgtacga actttttctg ctattgtttt attggtttgt cataattagt gagcttttct
     4861 tcaaaagaaa aattcctaga aacaaaagtc attaagactt tttattaaca aggctaaccc
     4921 taacgctcct atcagtttca ttcatttttt atcccttgat aaccgaatgt ctgcccattt
     4981 tttctactac ttggctcttt cttacggaat tgctttcttc tgctccgtcg gtattgatca
     5041 aaagaaattt tttattatac cattgggact tataacgcaa ttttgttatt aatttatgcc
     5101 agtcgtgaat agaaaaatca gcatgtgcct cttcttttgg aaaagattct acaaataagt
     5161 agtcatattg tttgaccaat ttcccagata attgttcaag ataatcttct cgctggttct
     5221 tttgtttcag ataaagatcc attacttttc tcttttgttt ctgataattg cttgcctgat
     5281 ctaacctgat ctttctatgg tgagcggagc ggactttcac attcaatttc cgctgtgcat
     5341 gttgcagctt ttcttctgtc tggtgaaatt ttggtaaagt tacttctaga ggtcgagatg
     5401 tttggatcaa tgtcttcgga tgataggcga tcccgatcca tttagctaaa gattcttttt
     5461 ttactggcga aacatcacaa agtagtgcga cataaaattc ttcattatat cgagcagaaa
     5521 tcgtagcgga gcgaatcgat ccttcaactg gccgatgctg atttatcttg attagttctt
     5581 tttgttttgg taactttaag tagccatttt ccagatagat cgtacgggtt tggttgttgg
     5641 tcgtataaga ttgccacata ctttttttcg ttttaagctt tgggaagctg gcacgtccct
     5701 tgaaataatt gcgaaaagcc cgatccaaat taagctgtgc atttgctaaa gcaagactgt
     5761 ctacttcttt taagaatggg tattcttttt ttaatgtagc gggtgtgagc gaaaaattgg
     5821 ctactcctgt ttgttgccaa cttttctgcc gctttttcag caaatggtta taggtaaatc
     5881 gagcacatcc aaaagtacga atcaattgct gtttttgttc ttctgtcgga tacatacgga
     5941 atttaaaagc ttttagcatc tatgtccctc ctagttagca accagaagag attccttttg
     6001 tcgttttagt ttttttgcgt aatatgggac ttcctgctca gttgcataga caaaatgttc
     6061 tggtgcgatt tcacgaagtt gacgtacttg tccgtcatct ggttcaggtt gatatttgat
     6121 tcttctgcgt tttctctcat ggtctggatc tggtagagga atagcagata acaaactttc
     6181 tgtataagga tgaacaccgt gattatagat ttcatcactt gttcccatct ctaataacaa
     6241 gccgttatgc attacaccga ttcggtcact aatatgttta accatcgaca aatcatgtgc
     6301 aataaataga taggtcaatt gatgttcttt ttggagatct tgcaacaaat tgaccacttg
     6361 ggcttggatc gacacatcta gtgcagaaat tggttcatca caaatgataa aacgcggttt
     6421 tactgccaat gctcgtgcaa taccaatacg ctggcgttgc ccacctgaaa attcatgagg
     6481 ataacgagta ccgtggcttg gattcaatcc aacagttctc aatagatcat cgactttttt
     6541 tgcacgctct tcagatgatt tagctagtcc attaacatca attccttcgg caatgatatc
     6601 tctgactttc atacgaggat tcaacgaagc gtatggatct tgaaagatca tctgaacatc
     6661 atgccggaat ttcgtcaaac cttttttatc tttgatcttc gttatatctt gaccgtcgaa
     6721 taatatctca ccatcagtag gttcattcaa gcgaatgatc gtacggccag tagtggattt
     6781 tccgcttcca gattctccaa ctaaaccgaa tgtttctcct tcataaatgt gaaagctgat
     6841 gtcatttaca gcatgtactt cattcttttt tcctacattg aaatactgtt tcaatccttt
     6901 aacttcaagt agtttatttg gcatcagcaa ttcctccttg accgtaagta tgtttttgtt
     6961 tctctttaaa gatagcccag cgacgttgga tttcttctgg cggtgtcact ttgggtgcct
     7021 gcggagcaag tagccaagtt gctgccttgt gtgtttttga tagttcaaaa aatggtggtt
     7081 cttgttctgc atcgattttc atcgcgaatt catttcttgg atagaatgcg tctccttttg
     7141 gcgggttcaa taaatccggt ggagaaccag gaatagcata caatcgatcg ttcgcactat
     7201 ctaatgtcgg cattgaacca agcagtcccc aagtataggg atgttgcggg ttgtagaaaa
     7261 tttcttccga tgttccaact tcaacgattc ttcctccata catgacagcg acacgatcag
     7321 cgacattcgc tactacacca aggtcatgag tgataaagat aattgatgta tcgatttttg
     7381 tctgaatttc tttcagcagc tccaaaatct gtgcttgaat agtcacatca agcgctgttg
     7441 taggttcatc agcaatcagg atttccggat aacataccag tgcaatcgcg atcacgatcc
     7501 gttgacgctg acctcctgag aactgatgcg gataactgtt gatacgtttc tcagcttcgg
     7561 gtattccaac taatttaagc aattctaatg cagctttttg tccctctttt tttgaaactt
     7621 tattatgctt tctaagggac tcagcgactt gcttcccaat gggcatcgtc ggatctaaag
     7681 aggtcattgg atcttggaaa atcatcgcta tttctttacc acggattttt tgcatctctc
     7741 tttctgattt tccaacgata tcgttacctt taaagaggat ttgaccagca tctatattgg
     7801 cgttgcttga cagcaagcgc atgatgcttc tggtcgtcac tgattttcca cttccagatt
     7861 ccccaacgat cgccaatgtt tctcctttat tcaaatgaaa attgacaccg cgaatcgctc
     7921 gtacctttcc agcatacgta tcaaaagaga tttctaagtc tttcacttct aaaattttcg
     7981 tcatcgtctt cactctttca ttttaggatc aaaggcatca cgcaatccat cagcaagtaa
     8041 gttaaagcca atcatgataa ccgataaagt tactgccgga atccataata gataaggcag
     8101 atacaggaat gtcttgtacc cttcgctcaa catcgttcct aatgaagctg acggaggcgt
     8161 aagacctaaa ccgataaagc ttaaaaaggc ttcgaagaaa atagcagatg gaatactaaa
     8221 catcatttga ataataatga cactagagat attcggcaaa atatgtttga atgcaatttt
     8281 cattcttgat tcacctaatg tcaatccagc taaaacatat tcttgatctt ttaatttcat
     8341 tgtttgtgca cgaacgatac gtgccattgg tatccagttt gtgatggcca tagcagccac
     8401 gattgaaaaa attcctggtt caaaaacgac caacatcaaa atcatgacaa cgaggtttgg
     8461 aattcctgat aagatttcca aaaaacgttg catcacgtta tcgacacgtc cgcctaacaa
     8521 accagaaacc aacccgtaag ctacaccaat cgttacatct agtaatgcag caataaatgc
     8581 gatcagcaac gagattctca ttcccataaa taagcggctt aatacatcac gtcccaaacc
     8641 gtctgttcct aaaaagaaat ttacgtttga agggacgttt gcttgagcat atttatcaac
     8701 aagttcacca ccaaccattg cttttccatt caatccatcg atattgattc ccggaatacg
     8761 aggaggcaaa ttgatataag ccacgttttg ggcagttgga tcatgaggag aaacaaagat
     8821 tgtgatcaca gagatcaaaa tgatgaccaa tagtaaaacc attgaaataa cggcagcttt
     8881 gtttttcttt aaacggcgcc atgaatcttg gataaaattc aaagaaggtg cagtaatctg
     8941 ttcacgttct tcttcggtat ttttttgtaa tggaagaaat tcatccgcag ggatatctgc
     9001 aactgtttca taacgctttg gtatcatttc catttatcct cggctccctt ctgaaacacg
     9061 aattcttgga tcaactagac cgtacaatag atccacgatc aaaataacaa aaatcagcat
     9121 aaatgagtaa aggatcgtta cagccatgat cgtaggataa tcatttgttg tgattgactt
     9181 aacgaattgt tccccgatac caggaatagc aaagatattt tctaccacca gtgatccggt
     9241 catcaatgct acagctaacg gtcctaataa agtcatcaat ggaatcaaac tatttcgaag
     9301 accatgttta aaggcgattt cccaacggct caatcctttt gcttttgcta attccacata
     9361 atcactatgc agtacttcca ccatttccgt acggataaac cgagcagagt cagcaagcgg
     9421 cgacatggcc aacgcaattg ttggtaagat tgtgtacaca aagccatccc attttgcgat
     9481 tggcaaaact tgtaatttga ttgcaaagat atattgtaat aaaacagcaa atacaaagtt
     9541 tggaatggaa cgacctaata tcgctacgag tgtagaagat gtatctgccc atgtattttg
     9601 cttcatggca gagatcgttc ctaaaatcgt ccctaggact gttccaaaga taattgcctg
     9661 taaccctaac tgcaaggatg gtccgattct tccagctaac aaatgtgcga ctggctggtt
     9721 tttaaattgg aaggaaattc caaaatctcc ttgaagcaaa ttagaaagat aaatcccata
     9781 ttgtacgata actggtttat ccaaaccgac ttgtttattt agcatctcga ttgtttcagg
     9841 actcaaacgt tcctgattcg tatatggcgt gcccggcagc aattgcatca agaaaaaggt
     9901 gattgttgca attaaccaaa gcgtgatgat catgaaaaag acacgtttta atacatattt
     9961 gatataacta ttcactaatg tttcccccta gcttttgact tctgacagat gtttcgctac
    10021 attattagat aatgaaccaa aaataaagga gcaatcaaat gaaaaaaaaa tctatcccct
    10081 acgcagtagc ttttctactg attctggtta ttttaataaa aaatctcatc aaccattcct
    10141 ttacactcat tcaattatct aatgatctat tcttatggtc attgccgttt ctgattatcg
    10201 gcggtttttt atgggtcttt tcttcgggtt tcttcgatca ttttcagcga tcggtccatc
    10261 ttgcacgaac aaggaaccga aagaaaaaac cagaattttc ctcgctctcg tctgccagct
    10321 acggtatgta cagcttttgg ctgatcattg ccggaatact aattgctctt tctgctattt
    10381 tcatgctttt ttctttgtta ggataagtga atatgagaac gaggttgcga catttgccgc
    10441 agcctcgttt tttctcatgc tcacggtatt aacgattatt cttcaacgta tgtccatttg
    10501 taatcccatg aagcgcctgc cgcatggtga acgatacctt tgattttttc attcactagg
    10561 tgaccttctg ctttttggta caccggaacg acacccatat catcagagat gatcttgatt
    10621 gctttttgga aatcatccca acgggcttgt tcatcgccag cgtctgttga tgaagctgct
    10681 ttaactgctt cgtcatattc tgcattgctc cagcgaccac ggttgtatga attacctgtg
    10741 acaaacaaat cagtaaagct gcttgggtct gcatagtcag cagcccatcc gcctagtact
    10801 gcgtcaaaat cacccgaagt tgagcggtca agacgtacag agaatggaac aggtgttggt
    10861 ttgactgtta cgccatccaa gttttcttgg attgagtttt gaacatattc gattactttt
    10921 ttcgatgaat catcatcaga agccatcaaa tcaaattcta aagaatcaac gcccaattct
    10981 tttttcgctt tttcccaata ttcttttgct ttatctttgt cataagaaac taattttcca
    11041 gcttcttttg caaagtcttc gttcgttgtt ggacttttta ccatatctga tgggatcaat
    11101 cctgtagaga caactgatcc gtcacctagt acttgtttta ctaaagcttc acgatcgata
    11161 gaataagaaa tagcttttct taggttcaca ttgttgaatg gtgaattctt ttcacgttga
    11221 ttgaattcga tatagcttgt ccgcgcttct ttaacagaag tataagctgg atcgtttgcg
    11281 ttttgctgtg ctaactcacc agacaagatc acgtcatctg cttggccatc tttgaataag
    11341 ttcaatgcag tagaagattc ttttactaca ctgactttga tttcagaaag ttttactgta
    11401 tctttgtccc agtaatgatc atttttctta taagtccatt ctgtatctgt tcctggtcca
    11461 tcaaaatctt ctaatgtgaa tggtccatta taaacagctt tatcacttgc agatgcatag
    11521 gcatccccat tttcttccac tacggattgt ttttgtggga aaaatgatgg gaaagcaagc
    11581 agatattgga aatatggtgt ttgtttcgtc aatttgattt ctaattcata gtcaccaact
    11641 gctttgatcc ctagttctga tttatctttc tttccagctg taatgtcttc tgcattttca
    11701 actggcgcat acaggtaagc atattcagaa gctgtagctg ggtctactgt tcgttgccag
    11761 ccataaacgt aatccgctgc tgtaacagga tcaccatttg accattttgc gtcttctcgt
    11821 aatttgattt tgtaagttaa tccgtcttca ctgacttctg ccaactcgct agctcctgcc
    11881 ggctgtggtt tgctgtcttc atcaagacgg taaattcctt cataaacgtt attcaatgct
    11941 gtaaaactga ttgtatctgt tgcaactgat aagtcagctg taggcatttc ttgaggcacg
    12001 actaagttaa atacgcctcc tccatctgct gaaccgcttt ttgctgaact actgcttgtg
    12061 gctgaactgc cgccataaca tccagcaagt acaaggccgc ataccgcaac caaaccaaat
    12121 gtaaatttct ttttcatttt acgtacccct ttattggaat tttcagaata attaatattt
    12181 tctgaagttg tttaagagtt taatgaataa ttattaaaaa atcaacactt tttttgtctt
    12241 tttcataaaa taatgatttt ttcgtgaggt ttcaaggaat ttttcagaaa aaatagcttt
    12301 gtacaaggtt ttccatggtc ttccaaaagc cattaaataa ttttcgttat attgtctatt
    12361 attgccatac aaagataaaa aagaataaac aaaaagaaac tgtgacaaaa gtcttgtcac
    12421 agtc
//
LOCUS       DACEOE010000072        12241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEOE010000072 DACEOE010000000
VERSION     DACEOE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.074"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..196)
                     /locus_tag="IW774_001640"
     CDS             complement(<1..196)
                     /locus_tag="IW774_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HAQ1153988.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEK"
     gene            624..1187
                     /gene="efp"
                     /locus_tag="IW774_001641"
     CDS             624..1187
                     /gene="efp"
                     /locus_tag="IW774_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1153989.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            1673..2932
                     /gene="lysA"
                     /locus_tag="IW774_001642"
     CDS             1673..2932
                     /gene="lysA"
                     /locus_tag="IW774_001642"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1153990.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            2919..3719
                     /gene="dapF"
                     /locus_tag="IW774_001643"
     CDS             2919..3719
                     /gene="dapF"
                     /locus_tag="IW774_001643"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1153991.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            3722..4603
                     /locus_tag="IW774_001644"
     CDS             3722..4603
                     /locus_tag="IW774_001644"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1153992.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            4600..5316
                     /locus_tag="IW774_001645"
     CDS             4600..5316
                     /locus_tag="IW774_001645"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1153993.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            5322..6017
                     /gene="dapD"
                     /locus_tag="IW774_001646"
     CDS             5322..6017
                     /gene="dapD"
                     /locus_tag="IW774_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1153994.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6033..7034
                     /locus_tag="IW774_001647"
     CDS             6033..7034
                     /locus_tag="IW774_001647"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1153995.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEKPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7031..8392
                     /locus_tag="IW774_001648"
     CDS             7031..8392
                     /locus_tag="IW774_001648"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1153996.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            8352..9503
                     /locus_tag="IW774_001649"
     CDS             8352..9503
                     /locus_tag="IW774_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1153997.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            9478..10647
                     /locus_tag="IW774_001650"
     CDS             9478..10647
                     /locus_tag="IW774_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1153998.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            10822..12201
                     /locus_tag="IW774_001651"
     CDS             10822..12201
                     /locus_tag="IW774_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1153999.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         4123 a         2106 c         2683 g         3329 t
ORIGIN      
        1 ttttttcatt gatttttcct tgggcaaatc cttctttgat caaataactg cccaatctat
       61 ctttgatact tcctccagga tttaaatatt ctagttttgc ataaatagca gaacctttag
      121 gcatagaata gtgctgattg tcaaagcgat aaagcggtgt ggaaccaata gcttctgtaa
      181 ttgtcgagat aatcatgtgg gcctcctaaa aaaagataat aaaaaaagga gctgactatt
      241 aaatcagctc cttgaaagac atagagaaaa attgagtaga taaaaagata agttacctgg
      301 tactcattct atgaagggca attaatagac atgacaaata gtatgctgct tttatgcacc
      361 atacaacagc aacgattcaa ttgtttttgg ttgctgactg tcatgtgtat ccctccaagt
      421 aaaattttta ttaacgatag catgaaaaaa aataaaagtc aaaggcttga aagaaaaaaa
      481 tatttcgata gcatcgttta cccgtgttta tttttctgac aaataggcat gagaccttaa
      541 aagatagaga atactctagt tttctggccg gttgtctgat acaataaagt tgattatgat
      601 aaagaaaaga aggagcaaaa ataatgatct cagcaagtga tttaagagcc ggtatgactt
      661 tcgtacaaga cggcaaactg attaaagttc tggatgcaag ccaccataaa cctggtaaag
      721 gaaatacagt tatgcgtatg aaactgaaag atgtccgttc aggtgcaaca tacgacacaa
      781 cgttcagacc agaagaaaaa tttgaaaaag cacatatcga cacaaaacct gtacaatatt
      841 tgtacacgat ggatgataca gctttcttca tggatttgga aacatatgag caatatgaaa
      901 ttcctgtaga aacagtagaa gaagaaatga aatatatcct agaaaacatg gaagtaaaaa
      961 tccaattttt cggtagcgaa gtcatcggcg ttcaattacc aacaactgtc gtattacgtg
     1021 ttgtggaaac acaaccttcc atcaaaggtg caacagtcac tggttcaggt aagccagcga
     1081 caatggaaac tggcttagta gtcaatgttc ctgacttcat cgaaacagat gaattgttgg
     1141 aaatcaatac agcagaaggt tcttatgtaa aacgtgcgtc aaaataattc gctaacggat
     1201 tgataagaat ttgaaaacag agcttgacct cttgaaaggc agatcatcgg atctgccttt
     1261 caagaggttt tttgtggaat cagcaaattg aaaaataaaa aagtatttga ttttatttat
     1321 attttgaaag atgaatggca aaagctagag aaagaaaaaa ctgcacttat ttatgaagaa
     1381 attgaaatga ttcataaaat tgaaaatatc cgaactattc cgaactatat tgaagcgaaa
     1441 atattttttc atcactaaat agtgttgaat ttatgttttt atgtataaat agcttctaat
     1501 agttcgggta ttttgttttt ataaccattt ttattcttat ttttaaaaaa atatttcgaa
     1561 atatactaga caattcagaa aattttgttt aatattattc caataaaatt caaatcttta
     1621 tcaaatgaaa ttccaataaa aacggaaaaa aagtgaaaag aggaatgaaa aaatgacaga
     1681 attgataatt ggcggagtca cagcaagcac attggcagaa aagtataaga cacctctgta
     1741 tgtgtatgat gaagaaaaga tgagacaaac gatgctgtca ttcaagcgag gctttgtatc
     1801 gaaacgtttt gatacaaaag tgctttacgc ctctaaagca ttccaaacga ttgagatgct
     1861 gcatttagtt cgtgaatatg gattcgggct agatgttgtc agtggtgggg aaatctatac
     1921 cgctttgcaa gctgattttc ctagagatac tatctacttc catggtaaca acaagacgcc
     1981 agaggaactg atctatgcac ttgaaaatga tttgttgcat attgttgccg ataatttgat
     2041 ggaagttgaa ttattagcag aattaagcca acgctattta aaaaaaatct gtatcatgct
     2101 tcggttgaat gtcggagtag aagcacatac tcatgaatat atcgtcactg cgcatattga
     2161 ttcaaaattc ggcatgagct atgaaagtaa agagtgccaa caagcccttc agttgataaa
     2221 agaaagcagc tatctagagt tagaaggatt tcatgctcat atcgggtcac aaatttttga
     2281 tgttactgct tggttagctg aaatcgacaa attgacgaat tacttaaccg attttgacca
     2341 acctctttcc ttgaatcttg gcggagggtt tgggattcgt tatacacaaa atgatcagcc
     2401 gatcccagtc gaagaggcac taaaaaaact gattgaatat acggaagaag ctttggaaaa
     2461 acgaaaactg accattcgtc aattactgat cgaaccgggt agaagtattg ttggagaagc
     2521 tggtacaaca ctatacacga ttggtttcat caaaaagaca ccacataaaa aatattactt
     2581 tgtagatggc gggatgacag ataatatccg tccagctctg tatcaggcag aatacgattg
     2641 tgatttagca gcaaaactgt cggaagaaaa aacagagaaa gtgacgattg caggaaaaat
     2701 gtgtgaatca ggcgatgtgg taattaaaga agcttatttg ccgaaagcag aatcgggtga
     2761 tcttttagct gtttattcca cgggagctta tggatactcg atgagcagca actacaatcg
     2821 agcgacacga ccagcagttg tttttgtcaa taatggccaa tcaagactga ttgttcgcag
     2881 acaagcattt tctgatctac taagggggga agtttcctat gatccttcat aaatataatg
     2941 gctgcggaaa tgattttatc ttattagatt acgcagaaga cacagattat agtcaattag
     3001 ccgcttcttt atgcacaaaa gaaaaatatg atatagacgg attgatcgcc gtaaaaatcg
     3061 atccgttgga aatgatctat tacaacaaag atggcagccg cgcgccgatg tgcggaaatg
     3121 gcattcgctg ttttgctcga tacgtaaatg atcagaacat gatgaatgaa caggaattca
     3181 acgtagagac attagcaggc acaagaaaaa ttacggtgtt ggatccctca cctttttatt
     3241 gcagcgtaga tatgggagtg ccagatgatt ctccggaaaa aatcaaagct aatcaaactt
     3301 atccaataaa aaaccaggaa atagagatca atggtgaaaa agtaacgatc actagtttat
     3361 ttttaggaac gattcatacc gttgtttttg ttgaaaatgc gcgtgaggaa gtctggagaa
     3421 aaagaggtga actgatctgt catcatcctt tgttcaaaga aaaaaccaat gtcaactttg
     3481 tagaagtagt caatgaacaa gaattgatcg tccgtaccta tgaaagagga gtaggttgga
     3541 cacttgcttg cggtacaggt tgctgtgcct cttatgtggt tgctaaggaa actgggaaaa
     3601 tttcagcaga tcaggtagtt gttcacttgg aacaaggaga cttgatgatc tctggtgagg
     3661 atgcgataca aatggctggc ccagcagttc atgaatggac aagaatattg gaggaatgaa
     3721 gatgataaaa ggttcgatcg tggcgctgat cacgccgatg aatgaagagg gatcagtaga
     3781 ttatgctggt ttggaaaaat tgatccaatt ccatttagat gaacaaacag atggattatt
     3841 agttttaggt acaacaggag aatcttctac gctcacacaa tctgaggagg agcagatcct
     3901 tcaactgaca gtaaagaaag tggctggccg tgtgccggtg attgcaggag ctggaacgaa
     3961 taatacgaaa gaaacgattg aaaaagcgaa gcattttgct tctttggggg cagacgcact
     4021 tttggtcatc accccttact acaacaaaac aagtgatgca ggactagctg ctcattttac
     4081 agcaattgca gaagcctcac cgattccgct tattttgtat aatgttcctt ctagaacagg
     4141 catgtcgatt ccaatccatg tattagtaaa tttagcagaa catcctaata tcattggttt
     4201 gaaagaagcc tcaggagata tggcttatgt gatggatgcc gctcggttga ttggagaaga
     4261 attcttcctt tacagtggaa atgatgattt gatcctacca gtaatgagtg taggaggaag
     4321 cggtgtaata agtgtatggg cgaatatcca acctaaaata gtccatgaat tagtaaaaga
     4381 tacgcaggac ggaagatggc agcaagcaaa agaaaagcaa ttaaatgcac ttgaactgat
     4441 ccatgccttg ttcagtgaga ccaatcctat tcctgtcaaa gcagccatgt cattattaga
     4501 cttaccttcc ggacctctac gcctgccttt agtttctctt tcaaaagaga aaaagaaaca
     4561 attagctcag cttttgttaa aagaaaggac ggttgaaaaa tgaaaatcgg actgattggc
     4621 agtggacaga tgggaaatag agtcgaagaa gttgcacaag aaagaggaga tacggtcctt
     4681 cttttacgga cggcacctaa agaagcaact acgcttgctt ttacggaact gccagaactg
     4741 cttattgatt tttctcatcc agataattta gagaaaatcc tttcgtatag cttatcatat
     4801 caacttccgt tagtcattgg cacgacaggt tatacacaat cacagttcca agaaataaaa
     4861 gaacatgcca aacatgtacc tgtgatgtat agtgctaatt tttcgttcgg catcatggtg
     4921 atgaatcaac taatcaagca agcagctgca atgttgcaag gctggcagat cgaactgatt
     4981 gaaaaacatc atagtcgtaa aaaagatgca ccatctggta ctgcaaatat gctgcttcaa
     5041 acaatccaag aagtgcaaaa gctgcagccc gtatacaact ggcaagggaa aaaaagagaa
     5101 gaaaaccaaa ttggtgtaca cagtattcgt gcaggttctt tacctggtga acatgatgta
     5161 ttgtttgctg ctactgatga aatatttacg atcaaacatg aatctttttc taatcgaatc
     5221 tttgctgcag gcgcagtaga agcagcggac tggttgaaag atcagtcacc aggttattac
     5281 aaattagaag atttattaat ggacaaaggg gtgtaaaaaa cgtggaagct caagaaatca
     5341 tccgctacat tcaaacggca tctaaaaaga cgcctgtgaa agtctatctg aatgtaaccg
     5401 agccaatcga atttaaagaa agtaaagttt ttggtgaagg aagcagcttc gttgtttttg
     5461 gtgactatga gacgattgct ccggttttag aagctgaatc agaaaaaatc attgaaatcg
     5521 aaatcgaaac tgctgcaaga tacagcgcag ttcctttgct agatattaaa aaaattaatg
     5581 ctcgtatcga accaggtgct attatcagag atcaagtgtc aatcggcaac aatgccgtga
     5641 tcatgatggg agcgattatc aatattggtg cagtaatcgg tgaaaacacg atgattgata
     5701 tgggtgctgt cttaggtgga cgagcgacag tgggcaaaaa ctgccatatt ggtgcaggag
     5761 ctgtcttagc aggtgtgatc gaaccagcat cggccaaacc ggttatcgta gaagacggcg
     5821 tactggtagg tgcgaatgca gtgattgttg aaggtgtcca tatcggaaaa gatgcagtcg
     5881 tggcagcagg agcagtggtt ttagaagatg tggcagcaga aactgttgtc ggtggaatac
     5941 ctgcacgtgt attaaaaata agtgatgatc agacaaaaga aaatacagca ttgattgctg
     6001 cgttacgcga attatagagg agatggagaa aaatgcgcga aatagatacc tgtattgtgg
     6061 gggcaaccgg attagtcggc caaacgatgc tcaaagtgtt agaagaatat gattttccag
     6121 taggacgatt gaaattatta gcttcaaaac gttcagccgg taaagtttgt gtattcaaga
     6181 aaaagaaaca cactgtacag gaattgacag aaacttcatt tgaaggatat gatttagcgc
     6241 tattttctgc aggtgcttct gtatccaaag agtttgcgcc aatcgcacga agtcagggac
     6301 tatttgtcat agataattcc tcagcatggc gggaagattc ggatattcca ttaatcgttc
     6361 cagaggtcaa tctggaagac tatacattga atcggctgat tgcaaatcca aattgttcaa
     6421 ccattcaagc ggtacttccg cttaaagctt tacaagaagc ctttggtatc cagcgagtca
     6481 attattcgac ctatcaagca gtttctggtt ccgggcaaaa aggcattgaa gatcttcatc
     6541 gtacgcaaaa tgacgagaaa ccagttttgt atccttataa tattagccaa acggtgattc
     6601 cggaaatcga tcagcctttg gaaaatgggt atacaaaaga agaacagaaa atgataaatg
     6661 agacccgtaa gattctgcat cagcctgctt tgccagtatc agcaacctgc gtacgtgtgc
     6721 caatcgaaaa tggacatgga gtctctgtgg cagttcaact gcaaaaagag tttacgacgg
     6781 aagaaattag agagtgtctc agccagtttc cagggattca attagaagat gatctagcag
     6841 cacataaata tccgacgagc attcttgcta gaggaacaga tatggtttat gttgggagga
     6901 tcagaaaaga cacttcttta gagaacggat tgttgttcta tacgactgcc gataacatac
     6961 gcaaaggagc cgctgcgaat gccgtacaga ttgctgcagc tttaatgcag aaaatagcgg
     7021 aggtggcaag atgagtaata cgctgaaagt agcgaaattc ggcggaacat cgatgtctca
     7081 tgaagagcaa ttcaaaaaag tccgtacaat tgttctttca gatcccaatc gccaagtgat
     7141 cgttgtttct gctttaggca aacgtacgtc agatgatgac aaagtaacag acttacttta
     7201 tttgatttat gcttaccttc agcatcatgt tgattggaca cccctttgga aaaggatttg
     7261 tgatcgtttc attcaagtaa gagatgttct tgccctgcag ttacctattg aagatgaact
     7321 agcacgcata cagaaacaac tggaagacga aaagataact gaagattatt tagtaagtag
     7381 aggtgaatat ctgactgctc aactgatggc tgagtatctt ggttaccagt ttattgacgc
     7441 aaaagacctg attttctttg actatgatgg gaaaatcgat caggaaaaaa cagcacagga
     7501 aatcacaaaa gcatttaaaa gatatccgaa aatggttgtt ccagggtttt acggggcgaa
     7561 ccctgcagga aaagaaaaat tattgggccg tgggggaagc gacattaccg gcgcgatact
     7621 ggcgaagatg ttgcaagcgg aaagttatga gaattggacg gatgtttcag gaatcatgat
     7681 ggctgatccg cgaatcatcg atcatcctaa aatcatcgag gaaatctctt ttagagaact
     7741 gagagagatg gcttatatgg gggctaacgt tcttcatgaa gcagctgttt tcccagttca
     7801 agaggctgat atccccatcc aaataaaaaa taccaatgat cctttcgctc aagggacaaa
     7861 aatatcgaat cacgaaatag aaaaggaaaa tggactgaca ggaatagctg gacgcaagga
     7921 ttttctttcc atcactttat tcaaacgtca tatgtccgat gaaatcgggt ttatctggaa
     7981 agcaatgagc atttttgcta aacatgagat atcaatcgaa catattcctt cagggatcga
     8041 caatatcgga gtgatcgtct cagcagaagc gattgccgat aagttatttc tcatcacgaa
     8101 agaattaaaa gaaacacttg gcgtggaaga aatcgtcgtg atcgaagact tagctttgat
     8161 ctctgtcgtt gggggtccgc ataaagaatt gattggatta tctgggaaag tgttatcgat
     8221 attgaacaaa ttagaaattc gcacatcgat cttatcacaa ggagcacagg agttgaatct
     8281 gatcatcggt gtaccgaata atcaatatga aacagtagtg aagggtatct atgaagggat
     8341 ggtgaatacg gatgctgaaa gaatatcgaa tggcactcca tcaaattcct gaaatcgggt
     8401 ttcatgaatt caagacaaaa aaatatttat atgaacaagt aaaaaacagc gaggccgtta
     8461 tccatgaaat cggcgaaaca ggactactcc tttattttga taatcagcaa gaagaaacga
     8521 ttgcttttcg aacagatatc gatgcactgc cagtcaaaga agaaacgaat ctctcttttg
     8581 cttctaagca tgaaggttat atgcatgcat gtggtcatga cggacatatg gcgatgcttt
     8641 tagggttgac tgactttata actaaacaca ttgaggatct tatttataat gtagtactga
     8701 tctatcagcc atcagaagaa atttccggtg gcgcacaaag cgttattcag tcaggattgt
     8761 tggataaata tgaagtacaa gcaatatttg gctttcatct atggcctgga ctagctaagg
     8821 gagaagtttt ctctcgacca ggacctttga tggcacaaag cagtgaaaca gacattatca
     8881 ttaaaggcca gtctgcacat attgcttcta gtgaaaaagg aatcgatagt ctggaggcag
     8941 ctgtacgatt tttgaagaat gtttatgatt ttgaccggca attaccggca gagtggaatc
     9001 atctaatgaa atttggccaa atccaaggtg gaacgatccg aaatgtttta gctggagaag
     9061 ttattgtatc cggaagtatc cgttcttaca gttcagatat gcaaaattat ttgaagaggg
     9121 gattagacca tttagcagaa cagtttcact ctacagctac tatttctttt cgctataatg
     9181 acggctatcc agctgtaatc aaccatagag gcttatatgc agcactgtta gattcaaaca
     9241 aactacatga attagatgct ccagttcttc aagccgaaga ttttggcata tatacgcaac
     9301 aatatccatg tgtatttttc tttttaggtg tcggtgatac ccctgcttta catgatccga
     9361 cgttcgattt tgatatggat gttttagaaa atggtgtaga atggtacaaa acgattcttt
     9421 ttacagaaaa tcttcttgta aaaatcgata gcagttcaaa agaggagcga gtaaataatg
     9481 atgaatttat ccaatcgatt tagtcctagg ctttctagaa tagaagtatc aaaaatacga
     9541 caattcgatc agcaaatatc gtcgatacca gatgtgatca aactcaccct gggagaaccg
     9601 gattttccaa ctcctgaaca tgtcaagcaa gcaggaatag cagcgattga agaagacttt
     9661 tctcactata ccggaatgag aggattagag gaactgcggg aagcggcttg tatatttcag
     9721 caacagcgtt atggattaac ttatgatccg caaacggaag ttttgacgac agttggtgca
     9781 acggaagcga ttgcctctgc gttgttatct gttttggaag aaggagacaa ggttcttatt
     9841 ccagcgcctg cttattcagg atatcagcca ttagtggaac tagctggtgc tgaattgatc
     9901 ccgatagata cttctgacac agggtttgtc tgtcagccgg aacaatttga acgagcattt
     9961 gaacaatatg gatcagctgt caaagcagtc attttaaact atcctaacaa tcctactggg
    10021 acgacattgt ctgcaaaaca attgaaagca atcgctgagg ttttaaaaaa atatccggta
    10081 tttgttataa gtgatgaagt atatgctgaa ttgacttata gtggaacgca tatgtccata
    10141 gctacttatc ttccagaaca aacaatcgtg ataagcgggt tatccaagtc acatgcgatg
    10201 actggttggc gagtgggctt tatttttgct caaaaagccc ttattgacga actaattaaa
    10261 gtccatcaat atctggtgac tgccgctaca acgatgtcgc aaaaagctgc ggctgaagcg
    10321 ctaataaatg gggtgaaaga tagcgagaat atgaaagagc aatatcaaac acgaagagac
    10381 tatctggtcc agcaattggc gcctttagga tttgaagtga cccagccaaa cggtgcattt
    10441 tacttgtttt gcaaactccc agctgccatt caaactgact cttggcagtt ttgtgtagaa
    10501 ttagcggaac aggcaaaagt tgcgtgtatc ccaggaattg catttggccc agaaggagaa
    10561 ggttatatcc ggatttctta tgctagcagc atggaaaaat tacatgaggc ctgcaaacgg
    10621 atcaaagact ttttaacaga acagtgattc atagatttta tcaaaaaaac aatcgttaat
    10681 tttgcggtat attctgcaag gagacgattg ttttttaaat ttagttcaaa tgaacgcgtt
    10741 tcttttgatt cataactaat gtaatgttaa gtttaaattg tttagaatga ttttaaaaga
    10801 atattcaagg aggtttcatc aatggaaaaa atcaaagttg tcattgtagg tgcatctcat
    10861 ggagggcatc aatcgatctt ggaactattg acacgatacg gtgaaaatgt agatatcacg
    10921 ctatttgaag caggagacta tatttccttc atgtcatgtg gtatggagct gtatttagaa
    10981 gacaaggtaa cagatgtaaa tgatgtacgt aatttcagac cagaaaactt tccgcaatcc
    11041 aatgttcata ttttaaataa tcacgaagta aaaacgatcg atgccgataa aaaaacagta
    11101 acggttacta gcgcatcaga taacgaaaca aaagaatatc cttatgacaa attgattttg
    11161 agttctggtg tcaaaccaaa caatctgcca gtaccaggag cagatctaga aaatgtctac
    11221 ttgatgcgtg gatacaattg ggcaaccaaa atcaaagaaa aattaaatga tccggctatt
    11281 aaaaaaatcg ctgtcgtagg tgcgggttac attgggatcg aagcagcaga agcaagccga
    11341 aaagctggga aagaagtcgt attgcttgat gtgatcgata gacctttagg cacttattta
    11401 gatgctgaga tgacagatat tttagaacaa cacctaaatg aaaacggtat tgaagttttg
    11461 acaaatgcaa aaatcgaagc ttttactggt aacggaaaag ttgaggcaat caaaacaagt
    11521 gaaaaagaaa tcccagcaga tctagtcatt caagctgccg gtgtgaaggc aaatacagaa
    11581 tggttaaaag ggatcgtcga cttagatgaa cgtggctgga tccaaacaaa tgaatacttg
    11641 caaacgaatc ttccagatgt gtatgcagta ggagatgcta cacttgctta ttctatccca
    11701 gcacgcaaaa aaatgccgat cgctttagcg actgttgcac gtagagaagc acgttatgta
    11761 gtaaaacatc tttttgaaga agtaccaaac aaaccgttcg gcggagtcgt aggttcctct
    11821 gcattaagtg tctttgacta tcattttgca gcaagcggat tgaatagctt tacagcagca
    11881 aaagcaggag tagatgtacg agtgtcttat tatgaagata cgttacgacc aaaatatgta
    11941 ccagaagaac aaggaaatcc aaaagtgtcc atccaattat tctcggatcc aatgacccat
    12001 caattgctag gaggagcagt attatctacc tatgatgtta caggtcaagg aaatgtattg
    12061 gcactagcga tccaaaacaa actgacttta gaagaactag cagaagctga cttcttcttc
    12121 cagccaggct ttgaccgaca atggagcatg ttgaatctag ccgctcaaca agcgttagga
    12181 gaagaaccat ttgtagaata agaaaacgaa atatttgaaa agaggttgtg aaaaagctgc
    12241 c
//
LOCUS       DACEOE010000073        12223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEOE010000073 DACEOE010000000
VERSION     DACEOE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.075"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            139..1230
                     /locus_tag="IW774_001652"
     CDS             139..1230
                     /locus_tag="IW774_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1154000.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            1260..1937
                     /locus_tag="IW774_001653"
     CDS             1260..1937
                     /locus_tag="IW774_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1154001.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            2269..3564
                     /locus_tag="IW774_001654"
     CDS             2269..3564
                     /locus_tag="IW774_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1154002.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            3554..5014
                     /locus_tag="IW774_001655"
     CDS             3554..5014
                     /locus_tag="IW774_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1154003.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            5150..6646
                     /gene="glpK"
                     /locus_tag="IW774_001656"
     CDS             5150..6646
                     /gene="glpK"
                     /locus_tag="IW774_001656"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1154004.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            6660..8483
                     /gene="glpO"
                     /locus_tag="IW774_001657"
     CDS             6660..8483
                     /gene="glpO"
                     /locus_tag="IW774_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1154005.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            8484..9200
                     /locus_tag="IW774_001658"
     CDS             8484..9200
                     /locus_tag="IW774_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1154006.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            9506..11435
                     /locus_tag="IW774_001659"
                     /pseudo
     CDS             9506..11435
                     /locus_tag="IW774_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            11561..12115
                     /locus_tag="IW774_001660"
     CDS             11561..12115
                     /locus_tag="IW774_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1154007.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         4087 a         2033 c         2780 g         3323 t
ORIGIN      
        1 caatgaatga tgcaacagat aagttacaaa aaaaataaaa ttttttaagc aaattagttg
       61 catgtacaac taatcgagtg gtttaatgat agatgattct cgataggtta atcagagcga
      121 attatgggag gaatgacaat gtcacaattt gatcaatcaa ttactttacc ttcaggagtt
      181 caattgaaga accgattgat gctgtcgcct atgacgacac aactcagctt tttcaatgga
      241 gtgatcaccg aagatgagat cacttattat aaacaacgtt ctaaaggtct tggtgctgtt
      301 attactggtg ctgcaaatgt ccaagatatc ggaaaaggct ggccaggtga actcagcatc
      361 gctcatgatg aaatgattcc tcgattaagt gagctagcga aagcaatcca aggagaaggc
      421 gcaaaagcga ttattcagat tttccacggt ggacgaatga caagtcgagc aactttatct
      481 ggagaacagc ctgtatctgc aagtgcagtt gcagcaaaac gtcctgatgc ggaaacacca
      541 cgagcaatga gtgaagaaga aatcgttgaa acaatccaag catttggcaa agccacacgt
      601 agagcgattg aatcaggatt tgatggaatc gagttgcatg gagcaaatac gtatttgatc
      661 caacaatttt tctctccgca ttcgaataga cgagaggatc aatggggcgg tagcttggaa
      721 aatcgttatc gttttattga agaattatta acaacggttt tctcagcggt tgaacaatat
      781 gctaaaaaac cattcatcgt agggtatcgt ttctcaccag aagaatatga aacaccaggt
      841 atccgttttg aagacacgat ctggcttctt gaacgattac gtgagtcaaa actagattat
      901 ctgcatgttt cattaaatat gtatgatcga atagctcgtt ctgataaata caacgataaa
      961 tcgattcttg aagtggtcca taacacgatt caaggaaaga tccctttagt tggtgtcggt
     1021 gggatccgca atcgcgaaga tgtagagcag gtattgaaat atgcagaact tgcagcaatt
     1081 ggacagcaaa tgatcgtcga tcctgactgg gcagagaaat tattggaaaa ccgtgaggat
     1141 gaatttgtaa cgaaaccttt tgaagaagca tataaagagc tttatttgcc aagcccatta
     1201 tataatttct tgaaagcacg ttatcaataa cgcaattttt atagaaagag gatgggaaaa
     1261 tgaaagtatt gatcgtatta acaaatgtag aaaagtacga aaagatcgag cgtcctacag
     1321 gtttgtggtt aagtgaactg actcattttt atgatgtatt agtaaaaaat gggatcgatg
     1381 cagattttgt cagtcctaaa ggcggatatg tacctttaga accgcaaagt gttttgaata
     1441 tggatgaaat cgattgggcc tattatgaag atgaagattt cagaaatcgg gccttgggaa
     1501 ataccttacg tcctgatgat gtggatgcaa atgattatgc agcggtttat tatgctggtg
     1561 gccacggaac aatgtgggat tttccagaag caatagatct tgggaaagtc gcttcacaaa
     1621 tctatcgaaa tgaagggctg gtctcagctg tttgtcacgg tgttgtagga ttgttgccag
     1681 ttacagatga gtctggaaaa tctattttag ctggacgtac agtgactggg ttcagtaatg
     1741 aagaagagga agcaaatggt acaacgaatg aagtaccatt cttagcagaa gatgctttga
     1801 aagaaaaagg agcaaactat cagtctggag cagctttttc agaagtggtt caaattgatg
     1861 ggcggcttct tacagggcaa aatcctcaat ctgctcatca attaggcgaa gcagttgttg
     1921 aagcattaaa gaattaaaaa taaatgatgt gtctattcta actattacat tacacacgct
     1981 aaaactcgtc ttttcgttct tttctgtttc ctatttggac agaggtacgt aaaaggcgag
     2041 tttttttctt ttataagagt acattttttg aaacatctct tcagaaatga tactcttcaa
     2101 ttaggtccac tagtacatta ttgagaagca gacctagaac acggtatgat ggagagggtt
     2161 gattctgctg catcgctatg tatcataagg ttgaaagcga gttatgaata ctgtgttttc
     2221 tgattgagaa gttagcagta gaacacctta taagaaagag gaatgcagat gaacattgta
     2281 attgtcggcg ggtcgtttgc agggattcat gcggctattt atcttcgcca attgatgcca
     2341 acaagtgaaa tatatttgat tgaaaagcaa tcgaagttag gatggattcc cagcgggttc
     2401 aatttgatat taaaaggaaa ggttaccagt gaaaaacaac tttcttggat cacgaaagaa
     2461 gaactgactg accgctatcg aatccatgtt tacacagaaa agacagtaat aggattgaaa
     2521 gaaaaaaaag tcgttttagc aggaggaaaa aaattggatt ttgatcgttt gattctagcg
     2581 actggttcaa atcaaaagtt tcgaaatatt tctgcagaat caacgcttat tcacccagtt
     2641 aaaaagatac aaaacgtaac ggctcttcag caaaagatca tgaatgcaaa gaaaattgca
     2701 attattggtg caggacaagc aggaatcgaa atagctgaag ggttggcatc aagaaaaaaa
     2761 caaatccatc tttacgaaag ccgaaagtca attctttttc gttatctaga tcctgaaatg
     2821 acagaaccgt tagtaaagga aatgaaaaat caagggcttt cacttttttt agaagaacaa
     2881 gttgtgtctt tgaccgaaaa tgaaaccgct attgtagaga cggaaaaaag aaaggagaac
     2941 tatgatcttg ttttgctggc taatcattct cgtcccgaca atcagatgtg ggaagaacaa
     3001 ttaacattga atgatgatgg aacgatctgg gtagatgact acttgcaaac gtctcaaaaa
     3061 gacgtttatg cgattggcga tgcgatccaa gtaacttttc gtccaacaaa tgaaaaaatg
     3121 tatgtttctc tagtaaataa cgctgtccgt acagcacgaa atgtcagcaa gacaattagt
     3181 ggctcacaga caaaggatca gggaacctat cgtccagtcg gcaatcagtg gtttggctat
     3241 ttcttaggaa gcgtaggttt gacagaagaa gagagcattt tttatcccca aaaaattgaa
     3301 acaggatacg tagactacaa gcttacagca ataaatcaaa gcaaagttaa actcaaattt
     3361 ctatttgatt ctgaaaaaaa gattcttgga gcacagctcc aaagcaaaaa agagatgttc
     3421 caccttcttg accaactgac aattgcagta gaagaaggat ggacgtttga acagttagaa
     3481 gaacatgagc tatttttcca gccagaatac cgtgtgccag atatggtgtg gtcaaaggtg
     3541 gcgaatactg cctatgaaga ttgaagcatt attagaaaga aaagagcagt tacagatcct
     3601 gattttgcga aaccttgttc tgcaaggggg aacagcctct atcaatggtt tgagagagca
     3661 tgtccaactg tcaaaagcct cgtttgatca atatttggaa gatattgaat tgattggccg
     3721 aatgatggaa aaaaaagtag aagtacagcg aaatgagtat caggcgctgc ttgtgttgga
     3781 cgaagatgta agtttggaaa aaattctatt gtttttattg caggaaagct tgaagtttaa
     3841 gatgctcgtc tatttattag aacatcagca agtatccatc gttcgattag cgacagcgtt
     3901 taatatcagt gagtcttctg tttttcgaaa gatcaaagaa ttgaaccagc tcttagagga
     3961 atttggatta caaatcaaaa acggtcaatt atacggagaa gagctacaga tacgatattt
     4021 ttattatgaa ctattccaat atatcccgga agatcagcga ccgctttttt tgcaaaatac
     4081 accagaaaaa agaccgttca ttctaggatt ggaccgtgta ttagaaacaa cgtttacggc
     4141 atcagcagaa gcacagattg cttgctggct cgggattacg aaaaaaagac tattgaatga
     4201 aaaaagtacg tacgcaactt tgaaagaaaa gaaattactc tatcagtcgg accggcttta
     4261 tcaagcaatc gatccaatca tcgtgatgca tcttagtcga acagcagcag aactgaatgt
     4321 atacgaaaca atgatgtttt acagcttttt tgtttctttt tcgatagtag acgaagaaat
     4381 attttatcag tatgatttga cacggagcaa aaagttacca accgctgttt tagatactta
     4441 cattcgggaa acgatgttat ggcattatcg tcctcgtcgt ttgaaaatca aagaagaaaa
     4501 ggcagtaggc taccaaattt cccagatcaa caatgaatgg tttttctttg ttggaaagat
     4561 cgaagtctat gagcgtgatc ggcttttgga acagcagcag aaaatgctcg ggcattcctt
     4621 aacacagtta ctggcgaaat tacaggaaac agcggtgcag caattgccga ggaaaagagc
     4681 tgaagatagt gagcttagtt acctgatgat ccagtatgcc aatgttcttt tgatgatcga
     4741 tttttatatt gcaaaaccag tagtgattgg aatcgattta gaaagtctgc cgatttatcg
     4801 tattgctttt caacaatatc tgatcagaga actgagggga atcggcggta ttgagattgg
     4861 aagttatgaa gaaggtaaag aatatgattt ggtgatcaca ttttgccaga gaaacaaaaa
     4921 acagtgtgaa tattatctgt cagaatttgc gtcaccttac gacattattc gattgaaaag
     4981 aagaatagag acgctgaaaa aagaaaagaa ttgattaaaa aatagcttca taataaaaaa
     5041 ctgtcaaaaa aagaaaaaaa gaataaaatt ttgacagttt ctttttttaa aacagtagta
     5101 tgataagagt agttaaagaa agcgtttgca tataatggag ggaatcacaa tgacagaatc
     5161 aacgtatatc atggcgattg accaaggtac aactagctca cgagcaatta tctttgacaa
     5221 gaaaggaaga cacatcggca gttctcaaaa agagttcacg caatatttcc cgcaagaaag
     5281 ttgggtggaa catgatgcaa atgaaatctg gaattctgta cagtcagtta ttgccggagc
     5341 gttcatcgaa tcagggatca aaccaaatca gattgctggg attggcataa cgaaccagcg
     5401 tgaaacaaca gtaatatggg aaaaagatac aggacgtcca atctatcatg cagttgtttg
     5461 gcaatccaga cagtcagctg gaattgcaga taacttaaaa aatgaaggct atcaagaatt
     5521 tttccacgaa aaaacagggt tagtgatcga tgcctatttc tcagcaacga agatacgctg
     5581 gattcttgat catgtagaag gcgcgcaaga acgagctgaa aaaggcgaat tgatgtttgg
     5641 aacgatcgat tcttggttag tatggaaact gaccgatgga caagcacatg ttactgatta
     5701 ctcgaatgcc agtcggacga tgttgttcaa tatccatcag ctagattggg atcaggagat
     5761 tttagattta ctaaatattc ctcgagtgat gttaccaaaa gtcacttcta actcagaagt
     5821 ttatggttat acgcaaggtt atcattttta tggaagcgaa gcaccaattt cagggatggc
     5881 aggagaccag caagccgcat tattcggaca aatggcattc gaaccaggca tggtcaagaa
     5941 tacttatggt acaggttcat ttattgtcat gaataccggt gaaaaaccgc aattatccaa
     6001 aaataattta ttgacgacaa ttggttacgg cataaatgga aaggtttact atgctttaga
     6061 aggaagtatt tttgtggctg gttcttcgat tcaatggctg agagatggac ttcaaatgtt
     6121 gcaaaaagct agcgattcag aagacgcagc gaaacgctct acaagcgaag atgaggttta
     6181 tgttgttcct gcattcgttg ggctaggtgc tccttattgg gatcaggcag cacgaggggc
     6241 tatgtttggc ttgacacgtg gtacgacaaa agaagatatc attaaagcta ctttacagtc
     6301 gattgcttat caagtgagag atatcatcga tacgatgcaa gatgacactg gaatcaagat
     6361 tcctgtattg aaagtagatg gcggtgcagc aaacaatgaa tatctgatgc aattccaaac
     6421 agatatcttg aatgtaccga tccaacgagc agaaaacttg gaaacaacgg cgctaggggc
     6481 agcattttta gcaggattag ctgtcggtta ttggaaagat acagacgaga tccgcgaatt
     6541 ttatgaagct ggcaagctat ttgaagttca aatggaggaa gagcgccgag aaaaacttta
     6601 caacggttgg aaaaaagcag taaaagctac ccaagcattt gaataaggag gaaatccata
     6661 tgttttcaaa caagacaaga caagatagca ttcaaaaaat gcagcaagaa gaattggatc
     6721 tgttgatcat cggtggcgga atcactggtg ccggtgtggc agtccagtca gcagcatcag
     6781 gaatcaaaac aggattgatc gaaatgcagg attttgcaga agggacttcc tctcgctcga
     6841 ccaaacttgt tcatggcggt attcgttatc tgaagacatt tgatgtggaa gtagtagctg
     6901 acacagttgg tgaacgtgca gtcgtacaaa gtattgcccc acacatccca aaaccagatc
     6961 caatgctttt gccaatctat gagggcgaag gagcaacaac cttcaatatg ttctcggtca
     7021 aagtagcaat ggacctttat gacaaactgg caaatgtgac aggaaccaaa tatgagaatt
     7081 atacgctgac accagaagaa gtattggaaa gagaaccgtt tttgaaaaaa gaagggctaa
     7141 aaggcgcggg tgtctatcta gatttccgca acaatgatgc ccgtttagtg attgataata
     7201 tcaaaaaggc tgccgaagat ggggcttatc tagtaagtaa aatgaaagcg gttggctttt
     7261 tatatgatgg cgatcaaatc gttggcgtca aagctcgtga cctgctgaca gatgaagtga
     7321 tcgaaatcaa agcaaaatta gtgatcaata cgagtggtcc ttgggtagat aaagtacgaa
     7381 acttgaattt tacgcgtcca gtctctccta aaatgcgtcc aactaaaggg atccatttag
     7441 ttgtagatgc gaaaaaactg cctgtaccgc aacccacata cttcgataca ggaaagcaag
     7501 atgggcggat ggtttttgct attccaagag aaaacaagac ttactttggt acgacagata
     7561 cggattacca aggagatttt acggatccga aagtcacaca agaagatgta gattatctat
     7621 tggatgtgat caatcatcgc tatccagaag caaatatcac attgaatgat atcgaagcaa
     7681 gctgggcagg acttcgtcca ctgttgattg gtaattctgg ttctgattac aatggtggag
     7741 ataatggatc gatttcagac aagagcttca ataaagttgt cgatacagta agtgaatata
     7801 aggaaaacaa agtttctcgt gctgaagtag aagacgtgtt gaaccatttg gaaaacagcc
     7861 gtgatgaaaa agcaccttct acgatttcca gaggtagttc tttagaaaga gaaccagatg
     7921 gtttgctgac tttatctggt gggaaaatca ccgattaccg taagatggca gaaggagctt
     7981 tgcgattgat acgccaatta ttaaaagaag aatacggaat agagacgaaa gaaatcgact
     8041 ctaaaaaata tcagatttca ggtggaaact tcgatccaac taaattagaa gaaacagtga
     8101 cagaattagc aaaagaagga gtagcagccg gtttagagga agaagatgct acttatatcg
     8161 ctgattttta cgggactaat gcccgacgta tctttgaatt agcaaaagaa atggcacctt
     8221 atcctggttt gagtctcgct gagtcagctc ggttacgtta tggtttagaa gaagaaatgg
     8281 ttttggcccc aggcgattat ctcattcgtc gtacaaatca tctgttgttt gaacgagatc
     8341 agcttgatga gatcaagcaa cctgtgatcg atgcaattgc tggatatttt gaatggacag
     8401 aagaggagaa ggcgcaacag actaaacgtt tagaagcatt gattgcagaa tcagatctgc
     8461 gggaactaaa gggggagaaa taaatgaaca gtgatatgac tcaaatcatg ggggagttta
     8521 ttgggacact gatcctagtt ttgcttggag atggtgtgtg tgcggctgtg aatttaaaca
     8581 aaagtaaagc acaggcttct ggctggattg tcatcgcatt tggttgggga ctcgctgtaa
     8641 caatggctgt ctatatttct ggattcatgg gcccagctca tttgaatcct gcagtctctc
     8701 tagctatggc gatgacaggg gcaattagtt ggaatcttgt cgttcctttc atcattgcgc
     8761 aagttttagg agcttttgca ggggccattc ttgtttggct ttcttatttg ccacattgga
     8821 atgcaacgaa agatgaaagc gcgattttag gaacgtttgc aacaggtcct gctatacgga
     8881 attatccagc aaatgtgatc acagaattaa ttggtacatt tgtattagtt cttgggttgc
     8941 tagcttttgg acaaaacgaa tttgcacctg gaacgaacgt attcgctgta ggtggattga
     9001 tcttagccat cggtctttca ttaggagggc caacaggata tgcaatcaac cctgctcggg
     9061 actttggtcc tcgattagca catgcggtat tgccaatagc taataaagga acttcagatt
     9121 gggcatattc atgggtacca atcgcaggtc caatgatcgg agcaattatc gcggtaggtg
     9181 tctattcatt aatggtgtaa atctataaaa catactaaag tttgacaacc aaaatgtgtc
     9241 ttgctatagt taagtggaaa ataaataaga cctagaagaa tggggtatgt tttgtgagtg
     9301 tagctggtat ctaatcagta agtcgaatag ccaataaaca aagtttattt ggtgtgctta
     9361 tgaaacggta cctgtcagga aaacaatatc tttgtattct aataaggata ggacatgact
     9421 tttttaagct gtgttctatc cttttattta tctctaggcc cttatttatt ttgggacagg
     9481 tatctcttca agaaaaggag agaatatgga caacgaaatg attagtcaat tacaatttga
     9541 tcggatcaaa aaggaaatcc aagcaagagc aattggaaac tatagcaaaa aacgaatcag
     9601 cgatatgacg gtctcgacaa acctacaaac tgtcttggat cgtcaagaag aaacaagaga
     9661 agcacgcttg atcttagaaa gcagtcagca tgttccattt atggggttgc cgaggatcga
     9721 tgcactgaca gaacaagtca aaaaagggtt gattctgcag ccaaccgatt tgatcgaata
     9781 tgcggatttt cttagaagca gtcggatgat cacaaagttt tttgataaga atcaatacca
     9841 gacacccttg ttgtttgctt atagcaaaca tcttcctgat ttgataaatg tagaagagtt
     9901 gattgaccag aaaataaaaa ataataaagt cagtgacgac gcgtcacgta atttgcgtaa
     9961 agtacgcaaa caattacaac taatcgaaaa agaaatccag tcaaaattac tgaaattctt
    10021 gcgtcatccg aaaaacaaag aaatgatcca agaagcaatg attgttcaaa aaggcgaata
    10081 ctatacgatt cctatcaaag ccagctataa aaataaaata gatggaacga ttatcgatga
    10141 atcaaataaa agaacgacgg tttttattga accaactgtg atttctaaat tgaatgaaca
    10201 ctatcagttg ttaaaagctg aagaaatcag tgaagaatat caagtcctgg cggcattgac
    10261 tggtgctatc gcagaaaacg aagaagtgat cgatttattg atcgaaacga tcacggtgtt
    10321 agatatcatc tttgctcggg caaaatttag ccgagaaatc aatggaagca cacctagaat
    10381 caataaatcg gaacatatca ttatcaagca aggcagacat ccatttttac ctgagcatgc
    10441 ggtaccattg gatgttgaaa tcggtaaaga ctatcgaggc ttgattatca ccggagcaaa
    10501 tgccggaggc aaaacagtcg tgttgaagac agtcggttta ttgactttga tggcgatgtt
    10561 cggtatgcaa gttccggcaa aagaagggac agagcttgct gtctttgatg agattttcgt
    10621 cgatgtcggt gaccaccaaa atttagaaaa tgcgttgagt acattttctg gtcatatgca
    10681 aaacatcgct gctattttga aaaagataaa aaggaatacg ttggtacttt tagatgagat
    10741 tggcagtgga acagagccaa atgaaggggc tgcactagcg atcgcaatca tggaatctat
    10801 gtatgagcaa ggggcattga tcattgccac gacccattat ggagaaatca agaaattcgc
    10861 cggagatcat gaagattttg tgccggctgc tatggctttc gatcgggaag cacttcgtcc
    10921 taaatatcag ttgcgggttg gaagaaacag gagaaagcca agctttgtgg attgctcata
    10981 aaatggatat gtcgatgaaa ttgatccatc aagcagagcg ttacttggcg gaaaaagcct
    11041 atcggacaca gaaaaaagaa tttcttccta agacagcagt tacaaatagg aaggagaata
    11101 aaaaagaacg tcagctcttt gcaaaaggtg atcggatatt tgtcaatgaa taccaaaaag
    11161 aagcgttagt ttatgaagat ataggtgaag atacgattga tgtatattta gacaaaaaaa
    11221 taatccacgt gcctcgtcag cgtgttcgtc tggtgcgttc agcagaagat ctgtacccga
    11281 caggttacga tttagacagt ctgtttatcg attataaaac acgtaaacgg cagcgtgacc
    11341 tagagcgagg atcaaaaaaa gctcataaag tactagtaaa agaaatgcgc aaacgacagg
    11401 aagaaagaag agtaaacgat gaaaacagca agtgattatc tcaaattagc tcataatatc
    11461 agtgtctgta aagaaaaata aaaacagtga aatcattggt tttattcccc gtgtaagcta
    11521 aaataaaaag aaaagagtgc tgaggaggta gggcgataaa atgaaattaa gcacagactg
    11581 gcgtaaagaa atacaaacga ttccgaatct tttatcgata ttccgtattt ttttgctgcc
    11641 gatttatctt tattttgtgc tgagacagtc attttatata gcaggagcgg tcattgttgt
    11701 ctcaggtttg tctgattatc tagacggggt cattgctcgg agatataatc aggtaacaga
    11761 cttagggaag gtgcttgatc cgtttgcaga taaattgaca caactttttc tgattttgtc
    11821 tatggcatgg tatcgaccgt ggctttggct gttgtttggt ctattcttga taaaagaagg
    11881 ctttatgttt gtagctggat taatcgggct atctaaaaat atcaaattaa gtggagcgaa
    11941 gtggtatggc aaagtagcga ctgctgtcat ctatgttggc atgatccttt tgctgctttt
    12001 cccagaatta ccaacgctgt gggtgagagt catttttgcc gtcatcactt acggtctttt
    12061 acaatctttt gtcttgtatg ctgtcgagta ccgaaagatg tttcaaagaa aataaaaaag
    12121 gggtgtgacc tatgtcgcat tccttttttt gataaataga ctcccagtag ctacttctcg
    12181 ataataagcc aaccgaacca aaaacaggag aagttgtttt tga
//
LOCUS       DACEOE010000074        12203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEOE010000074 DACEOE010000000
VERSION     DACEOE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.076"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(231..1958)
                     /locus_tag="IW774_001661"
     CDS             complement(231..1958)
                     /locus_tag="IW774_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1154008.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1951..3144)
                     /locus_tag="IW774_001662"
     CDS             complement(1951..3144)
                     /locus_tag="IW774_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1154009.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3331..3975)
                     /locus_tag="IW774_001663"
     CDS             complement(3331..3975)
                     /locus_tag="IW774_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1154010.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4069..4203)
                     /locus_tag="IW774_001664"
     CDS             complement(4069..4203)
                     /locus_tag="IW774_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1154011.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4205..6337)
                     /gene="feoB"
                     /locus_tag="IW774_001665"
     CDS             complement(4205..6337)
                     /gene="feoB"
                     /locus_tag="IW774_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1154012.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFFMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6334..6807)
                     /locus_tag="IW774_001666"
     CDS             complement(6334..6807)
                     /locus_tag="IW774_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1154013.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7208..7432
                     /gene="nrdH"
                     /locus_tag="IW774_001667"
     CDS             7208..7432
                     /gene="nrdH"
                     /locus_tag="IW774_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1154014.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7591..9750
                     /gene="nrdE"
                     /locus_tag="IW774_001668"
     CDS             7591..9750
                     /gene="nrdE"
                     /locus_tag="IW774_001668"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1154015.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9800..10765
                     /gene="nrdF"
                     /locus_tag="IW774_001669"
     CDS             9800..10765
                     /gene="nrdF"
                     /locus_tag="IW774_001669"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1154016.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10854..11993)
                     /locus_tag="IW774_001670"
     CDS             complement(10854..11993)
                     /locus_tag="IW774_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1154017.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
BASE COUNT         3743 a         2523 c         2149 g         3788 t
ORIGIN      
        1 gcacttcgta cataataaac tttaatcaca ttagtctttc atacctacga tcgatctctt
       61 tgactcgcac ccataaattc ataatagttt gtcggcgtct ttgaacgttt cgaagatgag
      121 ctacgcattc tcttttttta tagacaaaaa agatagagac gaacatggag cttccaaatt
      181 tggatatttg atccaatttt ggaagctcca tttatttcgt gtctgtcttt ttattttttc
      241 cagctttcga ctaattcttg gttctctttg atccagtctt ttgctgcttg ctgtggatct
      301 tttccattgt tgatttccaa cataactttt tccatatctt tttctgacca atggaaattg
      361 tctaatactt tgtaagcttc tggctggtct tctttcaatc cttttcgaac catcgtatgg
      421 atctgctctt cgcttcccat tccgttttca ggatcttcta agtatttcag atcatatttg
      481 gcaaacatcc aatgaggtgt ccatcctgta actacaattg gttcgtgttt tttgatcgct
      541 tgacctagtg caacggtcat tgctcctgaa gaagatgtct ctactttcca gtctttcaag
      601 ttgtcatatt tttggatcgt gttttcagca gccgtaacta caccagctcc tggctcgatt
      661 cctatgattt tcttacctgc ctgatcggtt aactcatcga tcgaatttac atccatgtag
      721 gctggcactg ctaaaccaac ttttgctcct gtcaaattgg cacctaggtc ttcgacttga
      781 tctttatatt gtgcatattg tgaaccatga gttttaggta gccaagctga aaccatggca
      841 tctgactcgc catttgaaac agatttccac atgattgaat tatctaatgg cgtcatcgtt
      901 acatcatagc ccatttgttt caatacttca ccgactacat ttgttgaagc aacttctgta
      961 tcccattcaa cataagataa gttgatacgt tttgtttctt tggcagatga aaaactccct
     1021 ataccaatcg cacctaagat cactactact acagcaccaa tgatcagtcc ttgacgtttt
     1081 ttacttttct gtgttgttgc tcctgcagct tttttattgt ttggctggtt caaatgctgt
     1141 gtaaaacggt caatgatgat tgctaagata actaatgcaa ccccgttgac aaatccattc
     1201 ccgacttgtg ctctttgcaa tgctgataat acgccgcgtc ctaagccggg tgcaccgatc
     1261 atagaggcaa taacgaccat tgaaagtgac agcattgttg tttgattgat ccctgccatg
     1321 atcgtacttt tagctaaagg caattctagt ttgaatagtt tttgacgact tgtgctcccg
     1381 aatgaatcag aagcttctac taattcttta ggaacttgtc gaatccctaa attcgtaaaa
     1441 cgaacggttg gtggcagagc aaagatgact gaggcaaata cccctggaac catcccgata
     1501 ccgaaaaatg caactgctgg aatcaaataa acgaatcctg gcattgtctg cataaagtca
     1561 aggatcggtt tgataatact ttgggcttta ctgctttttg ccatcaagat ccctaatggt
     1621 accccaatga tgatcgagat cacactagat aaaagaacaa gtgtgacagt attcatcaaa
     1681 tcattccata gaccttggtt ataaataaac catagaccga ttaatgtgaa caacgtaagt
     1741 cctggacgtt tcttagaaat aaagaaagct gctaccgtca ataacaagat aaacaaagga
     1801 gctggaatca ctaacagtaa atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt
     1861 aaaaatgaaa ataggccaga aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag
     1921 tttgccactg gtaattgata gttcaataga ttattcatgt tcactcacct cgcttgtttc
     1981 tgctaatgct tcaatcacac tacctcggat gacgacacct tttaattttc catcctctac
     2041 aactgcaagt ggagtcggtg agtcataaat aatattgaaa atatcactta ccaccatgtc
     2101 tttcccaatc accgtcacat tttcatctag gaagtcgatc aatggacgtt tttgcttcct
     2161 agcttctaac gcttgatctg cagtgatgat ccctttcagg tgtctctttc tgtcaacagc
     2221 catcagcatg ctgacttctt cattacgcat ccttgtcaat gcaacgtttg gtccatcaga
     2281 ttcgatattc gtcgtcaatg caggtaccat gatgttttga gccgtaagca ctttggaacg
     2341 atcgacttct tccacaaatt cacgtacata atcatttgct ggattggtca atatttcttc
     2401 acctgttccg atctgcatga tttcgccgtc tttcatcaag gcgattctgt ccccaatccg
     2461 taaagcttca ttcaaatcat gggtaatgaa aataatcgtt ttttgaacat tggcttgcag
     2521 gtccagtaat tcgtcttgca tttctcgccg gatcagcgga tcaagagccg agaacgcttc
     2581 gtccatcagc aagatctcag gatcatttgc taaagctctg gctaggccaa cccgttgttg
     2641 catcccacca gataactgac taggatattg gtctttaaat gaaagcaaac tagaattttc
     2701 taatgctttt tctgcttttt cttgtcgttc ttctttaggt acaccacgga cttctaaccc
     2761 gtattctgta ttttccaaga tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat
     2821 attgatttta tgacgacgga cttctcgcaa ttcttctttg cttaacttcg atacatcttc
     2881 tccatcaata tagatactgc ccgaagttgg ctcaatcaga cgattcaata gacggatcaa
     2941 tgtggatttc ccactcccgg aaagccccat gatcacgaag atttctcctt cttttacgtc
     3001 aaaattcacg tcatacacac caaccgttgc accggttttc tttaaaatct ccgttttcgg
     3061 ctggtgatca ttcatcatat ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa
     3121 atgttttact tctactttta ccaaacaaat tctccttttt tatgtaaatt catttttctt
     3181 tctgaaaaaa gtgaccacac tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga
     3241 aatttgcttg tccaaactga aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc
     3301 aagttaatat ttttttaata tttcacacaa tcattctgca agctgatgct ccgggtaaaa
     3361 aaagtttgtc atacgctgta gtgtatattc atttccaata aaatgaacga tgtcccctgc
     3421 cgtaaattta gcataggggc caggtgaaac aagcagttct tcattatgag aaattgcgat
     3481 aactgtggcg ccagtcgcct gccagatatt caactcgcta attgaacggt ccagatgcat
     3541 tgctttttct gataaagata attcaaacgg aagtaaagga ttcatcttat gtattcgctt
     3601 cgtttgattg accagctgat ccaataattg agaaaaatga ttcagctcat cttgttgctt
     3661 ctccacactt gccatgattt ctgcttttgt atcttggatg gattgtacat cttgatattg
     3721 caataggaaa tcttttgctt tttccttcga agcaacaaat gccccacttc catgccgtac
     3781 ttccatgatt ccaagatcta ccagcacatt gatagccttg cgggtagtct caggagaagc
     3841 attgtacgta ctggcaagtg ttgagcgggc atggatcttc tcacccactt tatatcggtt
     3901 ttcaacgata cgttcggcta aatcaacagc aatctgctgg tagcgcgggc ggacgatttt
     3961 tttctttcca gacattttct ttcctctttc tttttttact ttccctcatc ttactatgct
     4021 ctagcaaaat aagcaaaaaa ggggagaata gcctcttgca acgactgatc atcgcttgat
     4081 ctgttccttt tttactgggc aagctgtttg acagtcatca caattcttac cggattttaa
     4141 acgagtatag acaattgcgc ctgcacttcc aaagattaac acacttaata agaatgtcgc
     4201 catcctattc ctctccttct aatgacaata tagtataaaa ctggttcttg gattttggct
     4261 gacggaacaa aacataaacg agtcctatca tcagtaaaat agcaagatac gtccctaacg
     4321 ctacctgtcc accctccaat aagtgtccca actgataaat cacaaaacta actaaataag
     4381 ctaaaccgca ttgatagcct atggcgatcc aagtccattt cttggcattc atttcccgat
     4441 ggatagcccc aattgccgca aaacaaggcg cacaaagtaa attgaaggct aacaatgaat
     4501 aagctgctgc gggagtatat gtgtgttgta gcacttgcca gatttctctg ccattttcgg
     4561 atacttccgc atgaccaaac aaaataccaa atgtgccaat aatattttct ttagcgacca
     4621 atcctgtaat cgctgcgact gttccttgcc agtttcccca accaagcggc tgaaaaacaa
     4681 ctgccaggat tcgcccgaaa aaggctaaga tactttggtc ttcagaaaca ggttgagcat
     4741 aaaaattata agaagagctg aaccagatca cgatactaga aacaaagata atcgttcctg
     4801 cttttttaac aaaagacttg ctgcggtcat aggtttgtct taggaccgct gaccattgag
     4861 gaaaatgata tgcgggcaat tccatgataa aaggagctgt atctccagca aacaaacgtg
     4921 tttttttcaa gctgattcca gagaaaataa ttgcagtcat accaagaaaa taggcagaag
     4981 gagctaccca agattgatga ggaaaaaagg cccctgctac taaggcaata atcggcaact
     5041 ttgcagaaca aggcatgaag gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt
     5101 caatcgtgcg actggccatg acgcctggca ctccacatcc tgtagcaatc agcataggaa
     5161 tgaaggattt tcctgaaaga ccgaatttcc tgaaaagtcg atccatgaaa aaagcgatcc
     5221 tcgccatata tccgcagtct tccagaaagc tcaagcataa aaacaatata atcaattgcg
     5281 gtagaaatcc taacactgct cccacgcctg cgatgatgcc gttcaagatc agctcctgca
     5341 tccatcccgc tacttgccat tcagctaaca atctgccaac atgttctggt aaccatgtac
     5401 caaacaatgt gtcattgatc caatctgtcc ccattgttcc tactgtttga atagccagat
     5461 aatagatcag ccacatcaca aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa
     5521 tctgatcgat tttgtcactc atcgataatt ggaaagaatc atttgacaca acacagagtg
     5581 tacatagttg ggtaataaat tcataacgtt cattgaccag aatcgtctca ctatcgtcgt
     5641 ccaatagttt ctctgttaac tggatcagtt gctcgatctc tttttgctga gaaactgcta
     5701 tctcgtaatc ctctcttacc ttctgatctc gttcaaaata tttcattgca taccagcgtt
     5761 tcttgccgct ttgttctcct agtacatctt caatttcatg taaagctgct tctaaacgtg
     5821 gatcatatgt acagggaaca ggaatagaag gccgcttttt tgcagactcc tgaatctttt
     5881 cgattagttt ttcgattcct tgctttttca atacgctgat tggtaaaact ggtacaccaa
     5941 gaccatagga aagcttttct agattgattt tttttccttc ttttttgatg acatccatca
     6001 tattcagacc aatgatcata ggaatgtcta aatccaataa ctgcgtagtt aaataaagat
     6061 ttcgttctaa attggtagca tcaataatgt tgatgatcag ttcaggctgt tcctgaacca
     6121 aataatctct tgcaatcact tcttctggtg tataaggaga caaagaatag atgccgggta
     6181 ggtcctgaat atgaatgttc ggattttttt tcaacgatcc ttcttttttt tctactgtta
     6241 cgcccggcca attccctaca tattgattcg aaccagtcaa gagattgaac aagctagttt
     6301 tcccgctgtt tggatttccg actaacgcga tattcattct ttttcctccc tttgtatcaa
     6361 gattagttcg gcctctgact ttcgtaacgt cagttcatac cctcgcactg tcagttcgat
     6421 tggatctccc agaggggcta gttttcggat aactattttt acatttcgcg tcaatcccat
     6481 atccattaac cgtcgcttca ctgcgccttg tccgtggatt gctacgacga tcccagtctc
     6541 tcctgctttt aattgatcaa gagaagtcca gctttcagag gaagcagtct ttttattgac
     6601 aaagatttga cctagtattt cccgacttac tgccactcga tgacttttta ccagtaaaat
     6661 ggcattgttg cctgataaat tgacgacgac gattttactt ccggcaacga tcccgatatt
     6721 ttgcaaatgg cgttttgttt tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt
     6781 ctgctttgcc tgcgctaagg tctgcatatt gattcctctc ttgtgaaata aatcatgtat
     6841 taaaatcatt cattatatgt tctaattttc ttacgtgtta cgcaaataaa tgagaatcat
     6901 tctcacatag aatactctcg gtaattgaga atgtcaatta tacttgtgat attatgagcg
     6961 aatctttagt attgatagat aaaaatgaga aaaaaatatt tcttcatagt tttccacaat
     7021 tttttctcta ttaaatttgc tattttccca cacgatactt gttttcagct cttttttcaa
     7081 aaacacaaaa cttagtattc cacagtttcc acaacacaat atgtagtgtt tgttgtttga
     7141 cttttttact aagagtttta tactatctat tatagataat aagaatgtgt tagaggaggc
     7201 actcaccatg aacgtaaaaa tcttttctaa aaataattgt atgcaatgta agatggtcaa
     7261 acggttttta acagaaaatc aaattgcttt tgaagaagtc aatatcgatg aacagcctga
     7321 agcagtagac tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc
     7381 agaaacagtt atcggattcc gtcctgatca gttgcgtaaa cttgccagct aacatgacta
     7441 aaagaatcaa aacgtgatac atggatttga agggcttgag acagcaccag tttttgtcgc
     7501 aaacttccaa atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca
     7561 tcaaatacga aatgaaggtt gggcgtacgt atgagtctta aaaatttaca agatgtcagt
     7621 tatttcaagc tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag
     7681 gataaagaag ctttagttgc cttttttgag gaaaatgtca aaccaaatac gatgactttt
     7741 ccatctatta tggaaaaaat caattacttg atcgatcaag aatatataga agcagaattc
     7801 attcaattgt attcaccatc atttattgaa aagctatatc aatttttgga cgagcaacat
     7861 ttttctttca aatcttttat ggctgcttac aaattttatt ctcaatatgc gttgaaaacc
     7921 aacgacggtt ccgcttatct ggaaacttat gaagatcgag tagccttcaa tgcgctttac
     7981 tttgcgaacg gggatgaaga attagcactt gctcttgcag acgaaatgat ccatcaacga
     8041 tatcagccag ctacaccttc tttcttgaat gctggacgta aacgtcgggg cgaattggtg
     8101 tcttgtttct tgactcaagt cactgacgat atgaactcta tcgggcgctc aatcaactct
     8161 gctttgcaat tgtcccgtat tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa
     8221 gccggcgctc ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa
     8281 ttattcgaag atagtttcag ctattctaat caattaggtc agcgtcaagg agctggcgtg
     8341 gtttatttga acgttttcca tccagacatt attgctttcc tttcagcaaa aaaagaaaat
     8401 gcagatgaaa agattcgcgt aaaaactctt tctcttggcg tgatcgtacc tgataaattt
     8461 tatgaattga cacgtaaaaa cgaagatatg tatttattta gtccatatag tgtcgaaaaa
     8521 gaatatggtg tcccattctc ttatgtaaat attacagctg agtatgatca tctagttgca
     8581 aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa
     8641 ttacaacaag aatctggcta tccttacgta atcaatattg atacagcgaa ccgcgagaat
     8701 ccaatcgatg gcaagatcat catgagtaat ttatgttcag agattttaca agttcaaacg
     8761 ccttctgtga ttaacggcaa acaggaatat gaagtattag gaacagatat ctcatgtaac
     8821 ttaggctcga caaatatcgt gaatttgatg caaagcccta actttggtca atctgtgcgt
     8881 acgatgactc gtgcattgac atttattaca gatgcatctg atatcgatgt cgtaccaccg
     8941 atccaaaacg gaaataagct gaatcatacg attggattag gtgccatggg cttacacact
     9001 tatcttgcaa aagaacaaat ggaatatggc tcagaggatt cattagactt tacaaatatt
     9061 tacttcatgc tgttgaatta ttggacactt gttgaaagca acaatatcgc tcgtgaaaga
     9121 aaacaagttt tccataattt tgaaaaatca gcttatgctg acggcactta tttcgaaaaa
     9181 tacgtaactg gagaattcca gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt
     9241 tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa agatggactg
     9301 tatcatcaaa atcgcttggc agtagcacca aatggctcaa tttcatatat caacgatacg
     9361 agtgcaagta ttcatcctat cactcgcatg atcgaagaac gccaagagaa gaaaatcggt
     9421 aaaatttatt acccagcgcc ttatctatcc aatgagacga ttccgtatta cacttctgct
     9481 tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa cggcacaaca acacgtcgat
     9541 caaggcatga gtttgacttt attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg
     9601 aagacaacaa cgaagcaaac gacacgtgat ttgaatattt taagacatta cgctttccac
     9661 aaaggaatca aatccattta ttacgtacgt acctttactg atgatgcaga agaaatcgga
     9721 agcaatcaat gtgaaagctg cgtgatctaa tatcatagat agattcgctt attcatattt
     9781 taattaaagg agcattctca tgtcagaaac atattatgaa gcaataaact ggaatgaaat
     9841 tgaagatatc attgataaat ccacttggga gaagctgaca gagcaatttt ggctagatac
     9901 acgtatccca ctttcaaatg acctagatga ttggcgtaca ttgtctcaat tggaaaaaga
     9961 tacagtagga catgtatttg gcggtttgac actgctagat actgtccaat ctgaaagtgg
    10021 catggatcag ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt tgaacaatat
    10081 tcaattcatg gaatccgttc atgccaaaag ttattcttct atttttagta cactgaatac
    10141 gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg aatccttatc tgcaaaaaaa
    10201 agctgagcgt atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga aaattgccag
    10261 cgtcttttta gaaacattct tgttctattc aggtttttat actcctcttt attatttagg
    10321 aaacaacaaa ttggctaacg tagcagagat catcaaacta atcatccgag atgaatctgt
    10381 acatggaacc tacatcggtt acaaattcca attaggattc aacgagttac ctgaagcaga
    10441 acaagaaacg ttaaaagact ggatgtacaa tcttctttat gagctttacg aaaacgaaga
    10501 acgttataca gaagaattat acgatccaat cggctggaca gaagaagtca agacattcct
    10561 acgctataat gccaacaaag cattgatgaa cctagggatg gatccactct ttccagatac
    10621 tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa gcaaccatga
    10681 cttcttctca caagtcggaa acggctattt gttaggtcat gtagaagcga tgaaagacga
    10741 cgattatttg attggattgg aataaggatt tattttaatt caaaaatttt tcgaaaaata
    10801 aaaagagttt agcgtacaga tattttaaat gtacactgaa ctctttttta tgcttattca
    10861 cttgtttctt ccagcacttt tggactgtct aacttttccg aaacatgttc cattcttctc
    10921 aataaataga ttttccggat atttaatatt atcgtcttaa tcactgcata gacaggtatg
    10981 caaatcagca tgcctaatat tcctgcgaca cttcctgctc caatcaatac caagatgatc
    11041 gtcaaaggat ggatattcat tgatttccca aataacaacg gcttgatcaa atttccatct
    11101 aattgttgga tcactaaaat cgacaacgcc atatacaaag cttgaaatgg agagataaac
    11161 aaaccgacaa tgattgctgg taccgctcca ataaaagggc cgacatacgg gataatattc
    11221 gtgattccgc aaatgaccgc aagcaacaag ctataaggct gatcgaatat tctcattgcc
    11281 aaaaagctca ttacaccaat aatcaaagca tctaagatcg tactgctgat ataggcagat
    11341 aatgtttcgt ttaattcttt taccgtttgg cgtaactctt tacgaatcgc tttcggaaag
    11401 aagtgagaca acgcatcaat aaatttgtgc ccatccttga acataaatag caaaataaat
    11461 ggaacggtaa ataacaaaat aaaaaattgc atcataaatg aaatgatttt tgagacactt
    11521 gcagataaac tgactaatat gatattgaac aaattactaa gagataaatt tgctgatgct
    11581 aattgttctt ccaaattgaa atttttaaac tcttcccgct gcgccaattc atttaaccat
    11641 ttgcttgttt catctgcata cattggcaag ctctgggtca attgaattgt ctgttccatt
    11701 aactgaggca ctacattcat gacagttaaa acaactaaaa tgattacgac caaaaacgtc
    11761 aataaaaaac cgaacactct cgggatccga tgcttttcga gaaaaacaac gattggatcg
    11821 aacatataat agataaaacc agcaacaagc aatggtaaaa aaattgccga aagcattttc
    11881 aatattggac taaaaatatt cggcatttgt aaaataaagt acacgccgat gatcgtgagt
    11941 actagctcca ctgtccagaa aaataatttt gattgtttaa aacgtgattg catacatctc
    12001 ctccaaggtc ctttgatact tctattctat catttaacaa agagttacgc gctgagattt
    12061 tcatttgctt aaaaaaaaca aaaaaaataa acatatttta atgagtagac gagtacttaa
    12121 acaaaaacac ggtttggagt agtgacaaaa attatactgc agtccctttt tccaaataaa
    12181 cggtgttcaa aagcttgcat ctt
//
LOCUS       DACEOE010000075        12066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEOE010000075 DACEOE010000000
VERSION     DACEOE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.077"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(125..1432)
                     /locus_tag="IW774_001671"
     CDS             complement(125..1432)
                     /locus_tag="IW774_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1154018.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1435..2016)
                     /locus_tag="IW774_001672"
     CDS             complement(1435..2016)
                     /locus_tag="IW774_001672"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1154019.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2132..2229)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2588..3880)
                     /locus_tag="IW774_001673"
     CDS             complement(2588..3880)
                     /locus_tag="IW774_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1154020.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(3877..4500)
                     /locus_tag="IW774_001674"
     CDS             complement(3877..4500)
                     /locus_tag="IW774_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154021.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4503..5339)
                     /locus_tag="IW774_001675"
     CDS             complement(4503..5339)
                     /locus_tag="IW774_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1154022.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5356..6243)
                     /locus_tag="IW774_001676"
     CDS             complement(5356..6243)
                     /locus_tag="IW774_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1154023.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6259..7542)
                     /locus_tag="IW774_001677"
     CDS             complement(6259..7542)
                     /locus_tag="IW774_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1154024.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8225..8908)
                     /locus_tag="IW774_001678"
     CDS             complement(8225..8908)
                     /locus_tag="IW774_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1154025.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(8926..9543)
                     /locus_tag="IW774_001679"
     CDS             complement(8926..9543)
                     /locus_tag="IW774_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1154026.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9553..10377)
                     /locus_tag="IW774_001680"
     CDS             complement(9553..10377)
                     /locus_tag="IW774_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1154027.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10370..10930)
                     /locus_tag="IW774_001681"
     CDS             complement(10370..10930)
                     /locus_tag="IW774_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154028.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11104..12060
                     /locus_tag="IW774_001682"
     CDS             11104..12060
                     /locus_tag="IW774_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1154029.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         3628 a         2650 c         2046 g         3742 t
ORIGIN      
        1 gtgttcaatc agctgaccct cggtattagc tcaaacaaac ggcaaaatac gaggaactat
       61 ttttccgttt gtttctctaa caatcaggtc aaaacgctga tttcacaacc tttcttttgc
      121 tctattactt cttgaaggct tcttccaata tttcttcatc ttttttcaat ccattaaaga
      181 tcaaattcaa tatgatagcc gtaatgctac tcattacgat gccatttcca gtgaacattt
      241 gcaatgtttg cggcatttga ctgaataatg tcggcgtaac attgaaaccc agcccaaatc
      301 caacagaaac agcaacaatc aatagatttt tatcattgct gaaatcaact tccaacagca
      361 tacggattcc ttggactgcc accatcccga acatgactaa cattcctcca ccaagtaccg
      421 gctcagggat gatttgtgct aacgcaccga ttttagggaa taatcctaaa acaatcaaga
      481 agaatgcaga gaaatagatg ggttttcttg ttttgattcc cgaaagttga accaatccga
      541 cattttgaga aaagccagta taagggaatg tattgaaaat accgcctaag ataacggcaa
      601 gtccttctgc acgatatccc tttttcaatt cttcttctcc aacatttttc ccagtaatgt
      661 caccaagtgc aaaatataca cctgttgatt ctaccatgct gaccatggaa atgatgatca
      721 ttagtaaaat cgaccatacg tcgaaagttg gtttaccaaa ataaaagggt tgtggaacat
      781 ggaacaacgg cgcatctcca atcgcgctca gatcaatcat tcccatgaaa gcagctaaga
      841 tgcttcctcc gactaaacca atcaataccg cgattgaacg aatgaaccct tttgccagca
      901 tttgcacgcc tacaattagg agaatcgtca caaatgcaag tagcagattt gttttgtcac
      961 caaaatcagc tgctgttgca ttacctccac ccattttctc gattgctaca ggaatcaaag
     1021 tcaaaccaat aacggtgata accgttcctg ttactatagg agggaatagt tttttaattt
     1081 tagaaaagac accagaaact aacacaacaa agatcccaga agcgataata gatccgtaga
     1141 tagctcctac tccttcattg gttccgatca agatcagtgg agctactgct tggatcgcac
     1201 atcctaaaac gacaggcaag ccaattccga aaaatcggtt aactgtcaat tgcaataatg
     1261 tcgctaatcc acacataaag atatcaattg aaatcaaata agtcatctgt tcttgattaa
     1321 agcctagtcc tgtaccgatc aaaagaggca ccgctactgc tcctgcatac atcgctagca
     1381 gatgctgcag ccctaaaacc gcagcttttc cattttgtgt ttctttttcc acgattatgc
     1441 gtcctcctca agaaactcaa cttgtccatt cgtcaaggaa gcaatacgcg caagagaaac
     1501 gactctcaat cccatttctt caagtaactg acgtccatct tggaaagact tttcgatgac
     1561 gatcccgatt ccttctactt gcgctccagc ttgctggcat aattcgatca atccttttgc
     1621 cgcttgtccg tttgccaaga aatcgtcgat aatcaatact ttgtcttctg aagataaaaa
     1681 tttacgagaa atagaaatcg tacttgtcac ttgtttcgta aaagaataaa cagaagctgt
     1741 caataattct tcatccatcg tcaaactttt tgcttttcta gcgaagatca ttggaacatc
     1801 taattctctt gcagcaaata aagctggtgc gatcccagaa gcttcaatgg tcacaacttt
     1861 cgtgattcct tgttctttaa atacttctgc aaaccgtttt cccatttgtt ccatcaatac
     1921 agggtctact tggtgagtga cgaagctgtc gacttttaat acgccctcgc ctaatatacg
     1981 tccgtctttt tgaatacgtt caactaattc tttcacttta tttgctcctt tatcatttat
     2041 taataccgaa ataaccgctt ttgtgcaaaa acctactatg tttctgttaa agaaaaacac
     2101 cctccacttt atcataagaa aagaagggtg aaatagccaa tcttttactt atagtccagc
     2161 atttacggta ctggctagaa actgtcatcc atattatgac gatatataag taggtatgta
     2221 aaactaataa tagtgtaaat tcattttctt gcaactgttt tgcacgtgaa ggcagtttgc
     2281 catatttgaa taaaaaatgt tgtttcaaat attgaaaaca gtctaacact actttctgaa
     2341 actgacaacg tggtttactt atttttttgt aaaatctaat gatttttaat gcattattcc
     2401 tttttgttcg ggtttaaata gttattttta ttttctccta tctaaaaaag cagcccacat
     2461 aaaaagaagg catgacaaaa agcatgtcat agcccctttt tccgaataaa tagtgttcaa
     2521 aagacagcct atcggcaata aggcaagatt ttataaacta cgggaagtaa tttccgtaaa
     2581 tctattctta ttcttttaag ctgacaagct cgtcaatcac tactgctttt cctgaagcaa
     2641 tagaatgatt tgctgcgatt cctgtcaaaa tcgacaaaat accatctacg tgagttgctg
     2701 cccgattcca cggatcttct tctttttctt caccaaatag atcatctaat aaacgcggat
     2761 ctcctccgcc atgtccgccc gcttcttgtt cgacgattac ctcatatggc tcaccgaaca
     2821 tcggatagac atggatggac tgaagaacgg ctgctccttc atccttttta tctccacctg
     2881 cattgatgta agacttttcg atcactttca tttcgatccg tcctttgctg ccattgaaag
     2941 aaacattgaa tccttcccac ggcatatacg cgtttagcga atacgtcaaa atcgcaccag
     3001 tttcatattt gaccgttacg cctagtgtat cttcaataga gatatcatcg gaaaatacac
     3061 tgcggtcacg aatatatcca ctgtcttttt ctgcttctaa atacatttct cgcaattcct
     3121 cattggctga caggtctata gcaaatggat cagctttagc tagttcgctt ccatagcatc
     3181 gactgtaaaa tatttctact ccgcgtttct ccgcattatg catcccataa aagtttaatc
     3241 caccgaatgc aaataccttt tggggacggc tgttcaacca aaaattcact agatcaaaat
     3301 gatgggtaga tttatgaaca agaagcccgc cgctattgtg cttatttcga tgccatcttc
     3361 tgaaataatc tgccccgtgt tgcgtattta acagccattc gaagtggata gaaaacacat
     3421 caccaatcac atcactttct atcagttcct ttatcttcgt gtgatacggt gcataacgat
     3481 aattaaaagt gactcgcagg cttcttcccg tctgtttttg gaccgttatt atttcttgag
     3541 ctttttcctc atcgatagtc agaggttttt cgcaaataac atcatatcct tttttcattg
     3601 cacgaatgat ataatcatga tgcgttcgat ctactgacgt aataatgact tcagccggcc
     3661 gtacgtcatc cagtagttgc tcaaattgtg atggcataca tgtctttact tttttctgtc
     3721 catacttgtt cacaagtatc cgatttgcat aatccatcct tgtttgagat acatcacaaa
     3781 aaccgactag ttcacaagtt tgatgatacg tagaggcaat tgcctcataa aaaaaacgcg
     3841 ctctcccacc aagtccgact tgtacatatt ttttcttcat ttaaagtcat tctccttttc
     3901 ttttaagcaa aatactgtga caaatggcag ccctggatac agtaatgcag tgagtaaaaa
     3961 atcatgctgt atacaccaga acagtccata caaaaggcat aaataccaca gaaacccctt
     4021 gacgtttttt gacaacagcc agatattcct tttgatcaaa taaacaagac tatctgcttt
     4081 atcttctttg cagatatcaa ttagcgtact aaaaaaacta tgggatacaa aaagcaacag
     4141 acacaataga aaaggaatca gataccaagc tccaatcttt cccactaata aataccaatc
     4201 aactgcaaaa ataatgacaa acagctgaaa caacgtaatg atgaaacaca tccgtttgat
     4261 ttttcctttc ctccaacata cctttttttc tgccaagtaa tggacagata gataataaga
     4321 aggatacatc atcaaaaaat aaatcagata gacaacacga gcagacagca ctaatcctaa
     4381 agtgaacaaa catcctagaa ctaaagcgaa aagagcatta ctgataagac tggttaaaaa
     4441 aatccgacag acatagtagt tgagcttgta aagaaacatc tcttctattg gctttttcat
     4501 attcagccct tcagaccgct tgtactgatc ccctggacaa gatatttatt gaagaataag
     4561 aaaatcagaa aaaccggtag cagcgacaac gtagacatcg caaacatact cgcgtaagtc
     4621 gtactagatc cagcgtctgc aaataatttg atggcaatcg ataccgtgta agatctcggc
     4681 ttgtttaaat aaatcaatgg tcccatgaag tcgtcccatt tccaataaaa ctggatgatg
     4741 atcgtcgtga tgatcgaagg tttcagcaaa ggaaaaataa ttttggtaaa gacactatac
     4801 ttgctgcatc cgtcaataat cgctgattca tctaactctt tggggatgct gaccatgaat
     4861 tgcatgatct ggtaaatgaa aaatgcttgt ccaaaaaaat gtggtaaaat cagcggcaca
     4921 tacgtcccaa ccaaattcag ccgattgtaa ataataaact gcggaatcaa aatcacttgt
     4981 gcaggaatca tcatcgttac taacatgacg acgaaccaga atctttttcc tttgaaatcg
     5041 attctcgata atgcgtaagc aatacaagtc gagctcagaa ctgtccctat cgtcgaaact
     5101 gcagagatga caaacgaatt cctaaagaaa gtaaaaaatg aaattccagc aaaccccgcc
     5161 catccttttg cgtaattttc ccattgaaaa gtctctggaa tcaaattcag cgaaccactc
     5221 ataatctctt gatccgtttt aaaagaacct gcgatcatcc ataagaccgg ataaatcatg
     5281 actagaccta atagcaaaat aaaaccgtat tgaaaccatt tcgttactgt attattcatt
     5341 tcttcctcct ctcattcaca caacgaccca tttttttgat gatttgaaaa tcagaaaggt
     5401 gacagctccc ataatcaaca gcatgatcca cgaaatagca ctagcatacc ccatagaatt
     5461 aaaagaaaaa gcttggttat acacataaag agcataaaag ttcgtggcat cattcggacc
     5521 accttgggta atgataaatg cctgcgtaaa agtcataaac gccgcgattg tctgcataac
     5581 caagttatac aaaatgatcg gcgaaagaag aggtaacgtg atcttgtaaa acgtctgcca
     5641 tttgttcgct ccatcgatct ctgccgcttc ataatacgag cctgggatct ctttgatacc
     5701 tgcagcaaag atgatcattg ccgaaccgaa ttgccaagca gacatcaaaa caagagggat
     5761 catgaccagt ttttgatcgc taaaccagga aagcgcaggt gcgcctaatc gttccaggaa
     5821 aatattcact aacccgtttt tagaaaacaa ctcccgccaa actagagcga tagaaatact
     5881 gccaccgatc aaactaggca cataatacaa agaacgaaag aaagtcgttc ctttgatatt
     5941 cctcgttaaa agataagcaa tcaatagacc aaagatcagt ttcacaggta cagatgcaaa
     6001 tacatagaga aaagtgactc ggatggagtt caaaaaacgg gcatcactca gcatctttat
     6061 ataattatcc aagccgatcc atgagactgg atttcccaaa cgataatcgg tgaacgaata
     6121 ataaagtgac aagatcatcg gtatcactgt tatcaataga aatcctagga tgaatggaga
     6181 agcaaatacg taaccgacca cattgtcttg atccaagaac cgttttagtt tttctttttt
     6241 catgccttcc tcctgctgct atcctagaat gtttgacgca aactcataaa gactctctgc
     6301 tgcttcttct gctgttactt cttcataaat gactttttca actaaaagat tgtattggtc
     6361 ttgaatttcc ggattcaaag cactatcaaa gacattcact tcgccgttgt ccatatcgga
     6421 aatcatttct aaataatcaa atgtcgcttg caaagttgca tcatctgcac gagcgagctg
     6481 ttctcgaata ttcgacatga ttggtacacc acgttctcct tttaaaattt cattggcttc
     6541 ttcactgttt tgaaagaaat caataaactt agcagcttct tctttatggg ctgagctttt
     6601 tgccatactc agcatttgcg aagaacgtaa agggatacca gattctccat ctgcttttct
     6661 gcgaggtgga ttaatcaact gaatctccat atcttcagga gcccctgaca atatttctgt
     6721 caactggtta cttgatagaa acaccattgc cgcttctcta tccctaaccg cctgaaaaga
     6781 cagatccgtc gtacttgcag ctccaccacc tggttctgga tgggcccctt tgtctaccag
     6841 atttttacgc agctggaaaa aatctgttag atagtctgga ttttcaaatc ccaagctgtt
     6901 agatagatct tcccgattat aaaaattcaa tcctttctca acttgtggta tttgaataat
     6961 acagccagct ataaaatctt cgaatcttga aatagcatag attcctaatt cttcagtgat
     7021 ttgatagctg atttgttcaa attcatccca tgtccaattt gaagctggtt cagggatatt
     7081 taattgttca aatatatttt tattgtaagc aatcgcaaac gaattcaccc cgttagacag
     7141 tccaactaat tgcccgtcgt aagtcgtcgt tgctaaatag gattcatctg cattagaaat
     7201 atcaattatc ccttgctcca catattcatc taatggttca atctgattga tgtattgagg
     7261 aaattgattg ttcagttgga aaatgtccca gatatcatct gatgctgcta gtgtacctaa
     7321 ttttgtgtaa tatccttcat aatcataaaa ttcataatcg atcgtcacat ggggattttt
     7381 ttcttcataa agctcgatta cttttactgt tgcatcgtgt cggacttgcg aaccccacca
     7441 agccatgttc agcgtgattt ttttgtcatc ttcttgccca caagccgtca agaagaataa
     7501 cataacactt aaagctaaga tgattctcat tcttttcttc attctctgca cctctacttc
     7561 cattgttatt tttcaatata ctgtattttt atacagaaca tatttattaa acgatctttt
     7621 tcaaggggaa taaaaaccaa gacttgcctt atcgttctgc ctaggatgaa caactcaata
     7681 ctttcaagaa aaattagata gtgctgattc ttctttcatc gctaaaaaca cttcttgctt
     7741 caatgatggg aggataatta cgcaaagaaa aaaggatatc agacaactgc ccaatatcct
     7801 tgacatagct aaataagcat gcttcttcgt gtgcgctctt ccctaaaaaa cagaaaaata
     7861 actagtaatt ctgtatgaaa ttaggtacaa tatacacgtc gatgcacaag cattgactat
     7921 ggcaacttga ttggtcagtt cccgctgact tttcaagccc cgacaattta gctgctacta
     7981 aattgtcggg tgcctttttt agtttttatt cgtttttcat tctatagaaa gcgcttcaat
     8041 aaatcaagac atttttttgt tagaatgcta ataataacaa aaggttttaa ctcttcttat
     8101 gtacattaca ggtaaaaaaa caaaaaaatt tctctttttt ttgaattctg aacaaatagt
     8161 actggagtac aaaagacgag gaagagccac tatctgctct tcctcgtctt ttgattctca
     8221 cctgttattc atgattcttt ttctcagttt cccgatattt ttccatttta cgactaaaaa
     8281 gctctccgct acttggtggc gtatatgtga tggcatttgc tcctgcttga atggtctcag
     8341 caatactttc ttccgttgga ccacctgtcg caatgatagg aagttcagga tattttttac
     8401 gaaaatggcg tactgcttct gcagtttctt tacctgcact gatattgata atcgaaatac
     8461 cagctgctaa cttttcatct atttctgtat atttcgacgt taccgtactg ataactggaa
     8521 tatcaactac tcgacaaact tcttttactg tctctaaagg cgtaggaccg ttcaaaacga
     8581 cccctaacga accttgcgct tctgcgaaca tgctcatgta ggaagaacga gcaccttgtg
     8641 tcaaaccacc acctacaccg gagaaaactg gaatgtcagc tgcctcaata atgcttttag
     8701 taatcgctgg atgaggtgta aaaggataca cagcaattac tgcattcgca tttgtattac
     8761 gtatgatcgc aatatccgta gtaaagatga tcgaacggat caattttcca aatatcttga
     8821 tcccgcttgc ttctaaaatg atttccggta ctcttacgat atcttttctc aattccgtca
     8881 tgatttctgg cgtaaattgt tctgccatca gccacttctc ctttgctatc tccggtcata
     8941 ctgattcaat tgatacgtct cgatgacctg gatgattttt tgcgcataac cggggtctgt
     9001 cgcataacct gcatcttgca atgcttgtgc ggcttcctga tagttggtgg ccgtaatcac
     9061 tttttcatac agcgcaggat tccaatctac tccttggacg aacagttgtg tatggtcatc
     9121 catggactgt tcccatgaat catacacttt gaagtctcct tggatagtta tccattcttc
     9181 gttaacaaat tcctttgttt ccaggcttac tttcttctga tccccataag ccttgatccc
     9241 gaaaagatta ttgtatttgc tagccaaggt actttttccc caatttgatt ctaatatagc
     9301 ttgtcctaaa atgatactag gaagcactcc ataaccatct tgtagttctt gggcatgcgg
     9361 aacaagccgt tcaataaatt gctgatggga ttgcgcttca ttttcttgct gattatcagt
     9421 aaaaggagaa gacagattct tcaaggaaaa aacaaaggct aagagtatca ctgctacacc
     9481 ggcaaccact gctggccaac ggatattctt gcgcttttgc tttttccact tttttttcgc
     9541 catgatcaat tcctactttt catttaattc attcagatat tcttctagcg tcaattggtg
     9601 tttggtgata tactcagcgt tttctttacc gacataacgt aagtgccaag gttcatatgt
     9661 gatcttagtt atatcttcgt gtccttttgg ataacgaacg ataaatccat actgcgcagc
     9721 attatctgcg atccatttag ctccaggctg atctccgtaa cttgcatcca atacagtaga
     9781 aggataactg ttgtaccagt tttgatcgac gacatcgact gccagacctg tgtggtgttc
     9841 gctgtatcct ggctccgtaa tcgtttcttt agtaatcttt gtcgcctcct cttctgatac
     9901 accttgacga ctcatcacat cttggacatt ttgttcgaaa acttgttgtt gggatgatac
     9961 agaccggtaa gctgagacca tcactaatgg aaatccagct gcttcagcag ccttggcaaa
    10021 tgcttcatat tctggagcaa tgcgtttgtc gatcaagtat ccgttactta atgatgccaa
    10081 ttgagtagac tcatcaattt ctttttctaa tttgtgatct ggaccaacca aaactagctt
    10141 ccaatcgtct gttttaacat ctggcaattg tttggacgta cgatcttttg tttctttgtc
    10201 ctttaccttg ctgctggttg tttgactgtt cttttcttta ctagcagttg ttttggtgct
    10261 tgttcgttcg actgcttgat tgccggcttg aacatgcgaa tcatcatcgc taaaaataac
    10321 atagcccatt gctgcgatca taatcacaac tgctccaaat cctaaaaatt tattcacttg
    10381 aaaaatcctt tcgttgttct tcacgttccc cattcgcctc atgaacgtaa gtttcttcca
    10441 gattttcatt cacccaatcg agaagttcga ttttttctcc ttcgatctgt ttttgaacat
    10501 ctgctggcag acgaaaattc aaaatatcat catagcccca ttcatcatat tgaatactta
    10561 ctttcaagtc aaggtatcta ccagcctgct gattattttt caaagggatc agctcgactt
    10621 ttgctgcttt gctgctaagc catttttgcg cgatgcgtgt tttgatttgc ttgtactctg
    10681 caacggcaac gtttcgtttt tcgggaaaaa taatcgtttg acgcaaaatc ttttgcgtta
    10741 gcatccattc ataatcagtg aaaagatttt ttactttctg catcgcttca gatggcagat
    10801 ttttctcgcc atctttccat ttttgccatt gttctggact gatcagcaag tattcatcta
    10861 aaaaattttt ttcagtttca aactgttcta ataatacact cataataaga tcaatatata
    10921 cttgatgcac gtttatcccc tccttttaac tattctactt aagttttgat caaaaagcac
    10981 ctcttaaatc gctgctttca ttttttttaa tgtttcaagt cctctcctct ttattcttcg
    11041 tgatttttgt tataataata gacagacaag aaactcaatc caactatgaa ggagcgtttt
    11101 cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta ccatcgcaca tgagtttgcc
    11161 gaaagcttca atcaagagac gagcgaacta acagcagttg cttccagaac ttcagaaaaa
    11221 gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt acggaagcta tcaagaaatg
    11281 ttagacgatg ctgaaatcga catcgtgtac attgctgtac caaatagaca gcatatcgac
    11341 catatcttgg cagcattgga agcaggcaaa cacgttctat gtgaaaaagc catcacgatg
    11401 aataaaaaag aactggctga cgctatgcgc ttagcagaag aaaagaattt gatcttagca
    11461 gaagcgatga caatcttcaa catgccactt tatcagcagt tacgctcaat aatggataca
    11521 ggaaaactgg gggctttaaa aatgatccaa gcacctttcg gcagttacaa agaaccagac
    11581 cctaaaaacc ggttcttcaa tccagaatta gctggaggag ctctgttgga tatcggtacg
    11641 tatgctgtat ctttcgctcg tttctttttg agctctcaac ctgaagtagt tgcctctaca
    11701 atggttcctt ttgaaacagg cgtggacgaa caatccgtca ctatcttgcg taacaaagaa
    11761 aatgaacttg ctgctgtcag cttgactttt caggcaaaaa tgccaaaagt cggtatcgtt
    11821 gcctttgaga acggttatgt gacgattgct gattacccgc gtgctgatcg tgcggagatc
    11881 ttatttacag atggtacaaa ggaattcatc gaaagcggga atacatctga agcaatgaat
    11941 tacgaaataa agaatatggt acacatgatt gaaggtgact tgcccaatcg ctctctcttc
    12001 ttgacaaaag atgtcatcga aatcttggat caaatgcaag ttctatggaa aaatatgtaa
    12061 ttttaa
//
LOCUS       DACEOE010000076        12063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEOE010000076 DACEOE010000000
VERSION     DACEOE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.078"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(147..488)
                     /locus_tag="IW774_001683"
     CDS             complement(147..488)
                     /locus_tag="IW774_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1154030.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(556..2727)
                     /locus_tag="IW774_001684"
     CDS             complement(556..2727)
                     /locus_tag="IW774_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1154031.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGATETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            2938..3795
                     /locus_tag="IW774_001685"
     CDS             2938..3795
                     /locus_tag="IW774_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1154032.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(3866..4723)
                     /locus_tag="IW774_001686"
     CDS             complement(3866..4723)
                     /locus_tag="IW774_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1154033.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(4708..7410)
                     /locus_tag="IW774_001687"
     CDS             complement(4708..7410)
                     /locus_tag="IW774_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1154034.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(7423..8712)
                     /locus_tag="IW774_001688"
     CDS             complement(7423..8712)
                     /locus_tag="IW774_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1154035.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            8862..9908
                     /locus_tag="IW774_001689"
     CDS             8862..9908
                     /locus_tag="IW774_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1154036.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            9910..12063
                     /locus_tag="IW774_001690"
     CDS             9910..12063
                     /locus_tag="IW774_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1154037.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         3317 a         2552 c         2200 g         3994 t
ORIGIN      
        1 tccttgtaac tcagagttaa acggtaggct cacgacctct tggtaaatgg tgttcaagag
       61 acagcctatc ggcaataaga agcaattttg tcatcgctca aggccgaacg tctctttcac
      121 accctctgtt gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac tggtgtcatg
      181 atgatacggt tcaagtcatt gatgattgta ctgaagcctt gctctttttg catcaagtca
      241 ttgattactt cttctgattg gattttcaca gccatttctt gtgctttcat tgcatcttcg
      301 tcgctgaact cttgtccact catttgtttt tcttgtaatt cgatttgcat cgcttggaag
      361 tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc ttctttcaac
      421 gctttgaatt ctggtaattc acggatctca cgttcgattt gattcgcgct atcgtagatg
      481 tttgacatgt tatgccctct tttcttttag tagttactct ctattgtaac atggggttat
      541 tcgttttcaa aggggctact ctccttgata tttccgccag aggtctttag ccgcttcacc
      601 aattccctga acgccttctt tcaccatatc gccgaagttt ttcagccctt cttttgcttg
      661 ttcaccatat tgattcaagt tatcttgcca atttccagac tctccattag aatttcctgt
      721 gtttccaact tgatctgcag ctaccacttg tcctcctgtg gcataggcat ctgctacact
      781 aaatcctgtt tttgaagtat acggaaggat gccttctgcc gctgatttga agactttccc
      841 gacaacattt ccacttgtcc cttccaaata gtgcagcttg ctggattctt cgtaacccat
      901 ccatgtcgaa atcactacgt ctggcgtgta accgatcatc cattggtcat ttgcttttgt
      961 ggcatcgaaa ttagtttctg tggctcctgt tttccctgct atcgtataac ctgcaggatt
     1021 cgcagcagcc gctgttccat ttgaaaatgt accaagaagc atgctggtca tttcatcggc
     1081 tgtttccttt gatatgacct gttttggttt tttgtttgtg ttatcgacga tcaccgctcc
     1141 agtcgaatca acgattttcg tgataagatg cggcgtatac atctgaccat cattggcaaa
     1201 tgcactgtaa gcacccgcca tgatcaatgg agatactcct ttttccagtc caccaagggc
     1261 taagccataa tacttatcag attttgctaa aggcaggccg aattcttctg ctttatcata
     1321 tcctttttgt aaaccgatct catgcaaaag ccatacagta ggcaaattca aactttgtgc
     1381 aacagcttga tacatcggta cttctccttg gtatgtcccg tcaaaattat gcgcatcgta
     1441 ataactttgc ggttcgtctt tcaagatact ggaaggcttg tatccagctt ccagtgctgg
     1501 tgtatatacg cttaatggtt tgatcgttga tcctggtgaa cgacgcatct gtgtagcaaa
     1561 gctgaatcct ctaaagacat gatctcctct tcgtcctact agtgcttgta caccacctgt
     1621 attcggatct aaagctactg aagcagattg gaccatggca ccatctgaag catttgctgg
     1681 gaataatgca ctgttttgat aagtagcttc taattgcttt tgataatcct gatctaaagc
     1741 tgtatagatt ttatagcctt tattcatgac atcttcttca tcaagaccat actcgttaac
     1801 tgcttcatcg atcactgcat caaaataata aggataacga taaccagaat ctgaattatc
     1861 gtatgtgtca ttaagatacg cgctgatatc tgtctggctt tctgcttgtt cttgttcttt
     1921 tgtgattttt ccgttttctt ccattacact tagtactgtg tttttccgat cattcgcatt
     1981 ttttgggtga tcgatcggat tataaatacc aggacctttc agcattcctg ccaaagtagc
     2041 agcctcagac agcgtaacat tcgttgcatc tactccaaaa tactttctgg aagcgtcttg
     2101 gacaccccat accccgttgc caaaataggc attgttaaga tacatggtca agatttcttc
     2161 tttgctgtac ttcttttcga tctcgattgc caaaaataat tccttcgctt ttcgatcaaa
     2221 cgtttggtct aatgtcagat acgcattttt ggcaagctgc tgagtgatcg tacttcctcc
     2281 gccccctgat gttccgccag acaagaccat tcggactgct gcacgggcaa tccctttgat
     2341 atcaaacccg tgatgctcgt aaaaattcct atcttctgtc gagattactg catcttggat
     2401 atagggagaa atcgcgttta attctacata tgttcctttt tgggcgtaca gtgtgccagc
     2461 ttcttcgttt ttcttatcat aaatgactgt cgattggctt aatcctgatt ttaacgtttc
     2521 gacatttgct tgttttgcta aaataaagag gtagatactt gtcactaaga cacagatcaa
     2581 accaatcagc aaaatcaatt tgttcacatg atattttttc cagatgcgct ttcgccatcg
     2641 atggaattgt ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag cagcttttat
     2701 cttttctaaa atgctttgaa aatccatttg cttcttctcc tgtctatttg attcaatcgt
     2761 cttattttac catagtgaaa gagtgttttc ttcctgctta agttgtagtt caatatgttt
     2821 ttttacgtta gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa gttttttctt
     2881 aaatatgttt taaaaatggt acaattagtc tgcgattgca ctgaaaggag tacacttgtg
     2941 aaaatcacga tcactttacc tatgaaccat cctgaaacaa caattcgcga cttgcttgaa
     3001 aaggaatggt tagttcctag aaaggttcga cactttttgc gtatacgtaa aaacgtttgg
     3061 atcaatcaac aacctgcttt gttccatcat gaagtaaagg gcaatgacca gatcacattg
     3121 ttattcgaag aaacagatta taattatcaa gaagttcaat taggagatgc gaagcacatc
     3181 cgtgttttat atgaagatga gcatttgatt atagtggata agcctgcagg aatgaagact
     3241 catccaaatg aaccgacaga aaacagtacc ttactcaatc atttagctgc ttatctcgac
     3301 actaaacagc aacgacctta cgttgtccac cgcttggaca aagaaacaag cggtgccgtt
     3361 ttatttgcaa aaaatccatt tgtcttgccg atacttggaa gaatgttgga acaaaaaatg
     3421 atctatcgga gatatcaagc catcgtatgg ggagaaattg ccaaagatat gaccatcacc
     3481 aataaaattg gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga taaaaatggt
     3541 cagagcgcct tgacacatgt cactgtcaac aaagtacttt ctgaacaaac acaagtctat
     3601 tgtgttttgg aaaccggacg tacacaccag atcagagttc atttagctca taagggacat
     3661 ccgatcgttg gagaccctct ctaccaaaat agaacagctg aacgactgat gctccatgca
     3721 ttagaactgc acatgatcca tccatttact caagaaaaaa tagtcggtaa agctttacct
     3781 ggattatggg aatagaaata aaaaagaaca gagtcttatc agcagataat gataagactc
     3841 tgttcttttt gtgtaatttg tctgcttact tttgatgttc gtgttctgct aacagcaaac
     3901 taccgataat cccactttca ttcggcagtc cccaacgaac gatatattca cttacttgag
     3961 gtgtttccat ataaccaccc atcaattcaa caaattgctg acggattttt tgaaggagat
     4021 gatcctgatt cattacccca ccgcctaaaa taatcttttc aggtgctaac gtcaacgtat
     4081 agttgatcag tgcttgagca atataataag cctgaatagc ccaaacagga tgatcttgcg
     4141 gcagatcttg ccctttgatc cctgtgcgtg cctcaataga cggtcctgct gctaatcctt
     4201 ctaaacaatc tttatgataa ggacaagtac cttcataatg gtcatctgga tggcgtttca
     4261 cccaaatatg tcccatttca ggatgtgcga tccctgaaaa aatttcttca ttgatcacga
     4321 cacctgcacc gattcctgtt cccacggtaa ggtaaataca actatttttt ccttgtgctg
     4381 ctccttgctt caattcccca tatgcagcag cattgacatc cgttgtccaa gcagcaggaa
     4441 tattgtagcg ttccttgatt gtgccaagaa aatcgaattg tgcccaccct tttttgggag
     4501 ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat accaatcggg ccgaatgagc
     4561 caatcccgat tcccttcaat tcataacgat caaaaaattc aaatacctga tccaatgttt
     4621 cttctggtgt tgtggtaggg atacttaatt tttctaaaat ttctccacca tctgaaaccg
     4681 cacacacaaa ttttgttccg cctgcttcta ttccgccata cattcgtttt cctcctcgaa
     4741 tccgattgtc cgaatctcat aaggttggat tattgggaca accgcttgct tcgttggttc
     4801 ttcaagcaga ttcaatatga cagtttttcc attgtcttta gttatttcca atttttctaa
     4861 atgactagac atattgaaac ctcttactac tactttatca ctaaacttgc tgcgtttcaa
     4921 agctgtaatt gcaaacgttt cactttttat cgttaaatat tgttgttttg aaatcaatgt
     4981 tccttcatga tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt atgcatgcaa
     5041 ataagtagag aatttttctt ctggtccatg tagttcaatg ctgtaattga accgatgctc
     5101 acctagacac tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt ctcccacact
     5161 gcgaagtagc gtcactgcga tttgtccgtc ttctgttact tcatattcat tcaaaccaaa
     5221 gtttccgact gttacaccgt actcttcatc atgaatattg acaaatgctt gctgatgttg
     5281 cggattcgtg gggttttccc aacttttaga cacatggttt gggcgcttca ccacttcaaa
     5341 aatactatct gcttcgtgat gttccacatg taactttgta ggaaacaaga cacgtaagcg
     5401 atgatctttc atctgattgt cgagagtcgt ttcaaagtcg atttttttac tatcttttct
     5461 gattgtcatg attgttttta tttgaaggat tcgtttttct ttggatcgtt ctgcttttcg
     5521 ataacgaaat tccacaacca tctgctgttc tttttctaaa cgctcgtctg cagaaacagg
     5581 gatttccatt tcctgtagaa tacttatttt tgctatttcc ggtgtatcta ctaatacttt
     5641 tacttcagag ttttctttgt tactggatag gattggctga tcacagaaag gttgtttaaa
     5701 gatatactca tttcctatat cgccgatatc ttcaaaaata tgcagattat tcaactttcg
     5761 tttgtttttg cgatcttcca ttgtaatggt accatttttt tcaatcgtca aatgtagagg
     5821 gccgttttcg atgattcgtc cactctgatg aatcattgtt tccttttctt ctgctttcgc
     5881 ttccccttct acaagcgcga atgtttccca agaaaatgct ggcatgtttt ctaatggtaa
     5941 agtcactttg atcattcttg ccatatatgg cacacggaaa cggtctttcg gcaaatcata
     6001 accaaattgg acagtttctt cagataaaat agctggtata gtggccccac tttttgtttc
     6061 tacatgatac ttacgtttag gcaaattttc taactcttga tatagttgtt caggtattcc
     6121 ttcagcgaat ctttttcttt ctaattcgat ggttatttct gcttcaccag ttttatccat
     6181 tcctgcagta tttacaatga caaatggaaa gctgtctttt gggaatccgg tcgtatcgat
     6241 tgctgcggtc agctgttcta aggaatcttg tgcaagatac tttccaactt catctgcttt
     6301 ttcaaaacga ggtatcattt cccgatggac agaatcgact gaacaaccgc aaatactgtc
     6361 gtgaggatga ttttgcatta atgtcttcca agcataatct aattgatcat ggggatagtt
     6421 tccactaact tcataagcca tcgtcgcaag tggttctgta atattttcta gctgtcgctg
     6481 gaccttggta ttccattgtt ttaagtaaac ccgagcagat gctgtattgg ctaaagtata
     6541 ccagccatcc gtttcttgac ttgtcaattc tccttctact gagcctaagt cttctggcac
     6601 atctttttga actgcttcca gatagtcagt aaaattagaa tgaataaact cgtaatctgg
     6661 aaacagttca tttgcgagat ggattgcttt tgatatatct ttttgtaccg gttgatgatc
     6721 aacaccattc atcatcaata agtggttcgt agaggcatat tgttctgcat ccgctaattt
     6781 ttgcttccaa aaggcaagag ctgcttcttt ttctactgga atctcattcc cgttgctata
     6841 ccaattggca aatagcagac caaagattgc tgtttgatca ggtcctttcc accacatctc
     6901 tgagtattgg gaagaatagt tttctgcttc caaaacttga ttatcaaagc caattggttt
     6961 tacaccacgg ccaaacgctg ctgctgaaat accagcttgt ttcattaatt gaggcgtctg
     7021 tcccatattt ccaaatgtat cgggaaaata gcctaacatg acaggggttc cccatttacg
     7081 gctttcttcc atgccaatca gcatatttct cacgttcgat tccgagctga taagaaaatc
     7141 atcttgcaag atataaaatg gtccaattct caattttcct tcattgattg cttgttggat
     7201 tgcctgtctt ttttctggcc ttacttgaag atagtcatct aaaataatcg tttgcccatc
     7261 caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc tctagtagat cgtctatcaa
     7321 ttcaatcagt cgcatgtgat gctgttcgta agccatgtac cattcacgat cccagtggct
     7381 atgagaaatg atatatactt tctttttcat ttacgatacc tcctactttt ctactctaat
     7441 atcaaagtaa tccatgacta attcgcaaaa catcatattt gcccaagaaa accattctct
     7501 cgtataattt tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg ttcctgcgtc
     7561 agttgctaca agtaaattca atatccgctc tttttcagat ttgtctgacg ttgtcattcc
     7621 ttccatagct aatgcaatcg gccaaacgta tttttctggc gtatgggagg atccaattcc
     7681 ttttgcgtac tttccttcat agaaatatgg attttcttta ctcaacaatg ttcttcttgt
     7741 ttgcagataa tcttcctctt gttcagaaca atatcccaag taaggtgcgg cgatcagatt
     7801 gggcacatta ctgtcatcca tgactgtggc gtttcccatt ccatctactt catatgcata
     7861 aatcgtttgc cctttttgat taaccgttcg tccgtgattc tgaatcccct cttggatctc
     7921 tttcttcaat ttctgtgttt gctgtacaat ttctttatca tcgagtatcg tttgaaaaat
     7981 ttcttccaca taacctaaca cgaccaccgc aaacatattg gatggtacca gatatccata
     8041 acggcaagta tcatcgctag gacggaaacc agaccatgtc attcctgtaa aagctgtttt
     8101 tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc gtacaaatgt
     8161 ataaggagag tgttcatgat cttgctctgt tttgaacacg tccaagattt ttttgatacc
     8221 ttcaataaaa gattgattga attgatcagt ttttcctgta tttttgtaaa gaagataggc
     8281 caactgtatt gggtagcata aagaatcaat ttcatatttt ctttcccaga tccaatcgtt
     8341 catttttgta tgatctgttt ggtggccagc accgttagct tcttcgttga aggcattcgc
     8401 atatggatcg atattgatat aaaaaaattg ctttttgact aacccgctga tcatgtcagc
     8461 taattcctca tcttcttttg caatcactaa ataaggacga acttgtgcag tagagtctct
     8521 taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat tttgcttttt
     8581 gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc agtccgcatg
     8641 gatatcccca catttttgtg tgatatcttc cataaatttc tgcaccgatt ttggtatatc
     8701 tttataagcc actaataaaa ccttctttct atttgatata tctttaaaga ttatacttca
     8761 tacagccaat tatgtctacg ctttcaaaaa atatcttttt atttactttt tcatccatta
     8821 tggtattatt tgaatatgat atatcattaa gaggtgttta tatgaatcaa ccattgtacc
     8881 agacaatatt taatgatttg caaacggcta ttttaactgg tgaattacct gtagacagcc
     8941 aactaccaac tgaaaaagaa ctttcacgta cctataaagt aagcagaatc acttccaaac
     9001 gtgcattgac tgaattagag caattaggac ttatttatcg cgttcgcggg aaaggcagtt
     9061 tcgttaaatc cgcttctccg gaacagattc ttgcttctcc tacaaaaaca cgacggattc
     9121 tgtttcttct tccatatgtc gctgatttgt ctgttggtga ttttagcaag ggattgaacc
     9181 ctgtgatgca agataaacag attgatgtca tgatgacgac gcttgacttt ctacaaaata
     9241 aaacagcaaa agagctaatc aatgaatttg acggattgat ttattatgct cttgatacag
     9301 aacaacatct tgacttactt tttgagcttt cattgaaaga attccctgtc gtcatattag
     9361 ataaaaaaat ctatgagtta ccttttccta ccgttctttc cgataatttc caaggcggtg
     9421 cgcttgctgc ctcttatttg atcgaacagg gacattcaaa aatcgcttat ttgtttggta
     9481 cgcagactca tccacaatcc gttcgacagc gttaccttgg ttaccttgaa gcattggaca
     9541 aagctggttt gactttccac acttcactgg atgacaaaag cgcgacatta gatgaattgc
     9601 tttcttatat cactaagtat gaagtaactg cttttgtttg tgaaaacgat gttgtcgcta
     9661 tccagaccat gaatctcttg agacagcatg gttatcatat ccctgaagat ttttcaatta
     9721 tcggatttga tgatatccaa gcagcagctc ttgttgaccc gccactgaca accatcgcac
     9781 aagattttga acagttaggt aagcttgcgg gagatgcttt ggtcacttgg ttagagacaa
     9841 aagatcgacc atccgatagt aaatttccag tttcattcat caaaagacaa tcaacaaagg
     9901 agaaataaaa tgaatatcgg tcaaatagat actagacatg caacagcgaa tcagccaaat
     9961 ttctctaatg ggaactgcca gccttatact ggcgttcctt ttgggatgaa ttattttgca
    10021 ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg cgtttttcaa
    10081 ggctaccgtc ttacccatca accaagtcct tggatgggtg atttcagtca tatgctgttt
    10141 acgccgatca atggaaaact acaggagaac acccttttcc atgctcaaag ttcttaccgc
    10201 ccagaagaaa gtatattttg cccgactcat ctgtcgatct gtcaattacg ttatggcatc
    10261 tgttctacat tgatacctag catgtatggc ggaatcttat ctattgatta cagcaaaaat
    10321 gacagcggtt tacttttatc ctttccggga cgccatcagc tctttgttat agatccttac
    10381 acagtggaag gaaaaatcac acattttgct ggttcagaag atcctgattt ttctttttat
    10441 tttatattga gatttgaata tcctttaaca attgatgagt tagctgtttc caacaaaaat
    10501 agcgactcga tcccgttgta ttttgaacag acaaaaaaac aaactatccg atttgggaca
    10561 tcttttatca gctcggaaca agcagcttat aacctggaac tagaacaaaa ccaaagcaaa
    10621 gagacttacc ttgctcacag tcaagaacaa tggcagtcct attttgatcg tatacagctc
    10681 acacaccatg atcaacaaca attgcggacg ttttatcata atttatatcg ggtgttttta
    10741 tttccacaaa cattttatga aattgatcct agtgggcaaa aaatccacta tgatacgact
    10801 agtcgatcta taaaacctgg tgttctctat acaaataatg ggttttggga tacgtataaa
    10861 acggtttatc ctctttattc tttgattgct gtagaaaaat acgaagaaat gctggaaggt
    10921 tttttgaaca gctatagaga aagtggctat ttgccaaaat ggctgtctcc tgatgaacgt
    10981 ggacttatgc ctgggacatt gatcgatgcg gtcattgcag atgctgctgt aaaagatatc
    11041 cgacgagact tgatgcctga atttctaacg gcaatggaaa aaggtgctac ggttcaaagc
    11101 aaacatccaa attatggacg ccaaggaaca gaagattact tgacatatgg ctatgttcct
    11161 aatcactacc acgaatcagt caatcacaca ttagactatg cttatagcga tttttgtatt
    11221 agccaagttg ccaaggtact agaagaagag gaaatgacga agaaatatca gcagcaatca
    11281 ttgaactatc aaaatatctt tgactatgag tcaggattta tgcgttcaaa agataaagat
    11341 ggcaagttca gagaaccatt ttcagctaat cgttggggac aagattatgc agaaggaagt
    11401 gcttggcaaa gcagctttgc tgtttatcac gatttttcag gactgatcgg aaaatatggt
    11461 ggcagtgaga gatataaaga aaaactcatt gagctgtgca atcagccccc aacgtttgac
    11521 ccaggaggat atggttttga gatccacgaa atgagtgaga tggccgcgat agaatttgga
    11581 caattagcta tttcaaacca gccaagtttt cactatcctt acttgttcag ttatatagga
    11641 caacctgaaa tggcacagcc actgcttaaa cagttgatga ctcagacctt cgacgattct
    11701 ccaatgggtt accctggaga tgaggacaat ggaagtatgg ccgcttggta tatcttcaat
    11761 agtctgggat tttatcctgt cacgcctgga accggtgaat atgttatcgg tatgccatta
    11821 attgacaagg ctgttttgct tttgtcaaat ggaaaggaat tgacgattga agcaacgcct
    11881 aatcggccgc agcaacaatt tatccataaa atcaaacgga tggacaaaga atatacgaaa
    11941 ttatatttta cccatcaaga tttgctgaaa ggcggcacga tctcttacca attagggatc
    12001 atccctcacc ctaagaaata tcaagcaaaa gatttgcctt tttccttatc aaactataat
    12061 taa
//
LOCUS       DACEOE010000077        11725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEOE010000077 DACEOE010000000
VERSION     DACEOE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.079"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..305
                     /locus_tag="IW774_001691"
     CDS             6..305
                     /locus_tag="IW774_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1154038.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IW774_001692"
     CDS             378..1739
                     /locus_tag="IW774_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1154039.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IW774_001693"
     CDS             1761..2021
                     /locus_tag="IW774_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154040.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IW774_001694"
     CDS             2035..2787
                     /locus_tag="IW774_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1154041.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IW774_001695"
     CDS             complement(2881..3486)
                     /locus_tag="IW774_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1154042.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IW774_001696"
     CDS             complement(3539..4513)
                     /locus_tag="IW774_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1154043.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IW774_001697"
     CDS             complement(4891..5670)
                     /locus_tag="IW774_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1154044.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW774_001698"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW774_001698"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1154045.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IW774_001699"
     CDS             7161..8558
                     /locus_tag="IW774_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1154046.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IW774_001700"
     CDS             8639..9079
                     /locus_tag="IW774_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1154047.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IW774_001701"
     CDS             9227..9553
                     /locus_tag="IW774_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1154048.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IW774_001702"
     CDS             9554..10303
                     /locus_tag="IW774_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154049.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IW774_001703"
     CDS             complement(10383..10838)
                     /locus_tag="IW774_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1154050.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IW774_001704"
     CDS             complement(11265..11540)
                     /locus_tag="IW774_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154051.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3713 a         2026 c         2371 g         3615 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attac
//
LOCUS       DACEOE010000078        11665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEOE010000078 DACEOE010000000
VERSION     DACEOE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.080"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(347..2545)
                     /locus_tag="IW774_001705"
     CDS             complement(347..2545)
                     /locus_tag="IW774_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1154052.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            2777..3769
                     /locus_tag="IW774_001706"
     CDS             2777..3769
                     /locus_tag="IW774_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1154053.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            3829..5250
                     /locus_tag="IW774_001707"
     CDS             3829..5250
                     /locus_tag="IW774_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1154054.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            5243..6742
                     /locus_tag="IW774_001708"
     CDS             5243..6742
                     /locus_tag="IW774_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1154055.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            6781..8175
                     /gene="uxaC"
                     /locus_tag="IW774_001709"
     CDS             6781..8175
                     /gene="uxaC"
                     /locus_tag="IW774_001709"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1154056.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            8334..9017
                     /locus_tag="IW774_001710"
     CDS             8334..9017
                     /locus_tag="IW774_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1154057.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            9019..9855
                     /locus_tag="IW774_001711"
     CDS             9019..9855
                     /locus_tag="IW774_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1154058.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            9866..11620
                     /locus_tag="IW774_001712"
     CDS             9866..11620
                     /locus_tag="IW774_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154059.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         3686 a         2258 c         2196 g         3525 t
ORIGIN      
        1 tcgaagaatc tactcctgga acaccgtgta tcagaggttg tgaaagagac gttcagcctt
       61 gagcgatgat aaaaactctc acaaacattg cgacgagtaa cccacaggag caataagaag
      121 cgattttgaa aaatagcttc ccatatttca caaaattatg ccttattgtc gatagcctgt
      181 ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa gacttttgtc acagttcttt
      241 ttgcgtgctt agccttgaaa tttaactatc cataaataca aatactaggg ttattctaac
      301 ttcttttatt gcttgaatca gtccacaaga aaaacagcct cttgattcac aaatattttt
      361 ttcgatattc tcccggcgta atcgattcct tctttttaaa gaaacgaata aaattttgcg
      421 gatttcgata ttgcaatctt tctgcaatct cttttacgga gaggttagtc tctttcaacc
      481 atttttttgc tacctctaaa cgatgatttt gaacaaaatc accaaatttt tctcctgttt
      541 cctttttaaa tacattactt aaatagttag ggttataatg caacctatca gcgatcacat
      601 ctaaagacag atcacgatca tattctgtgt agacgatatg cataatattc tccgacaaat
      661 ttttaaattc atgatcagtg attgcttgag agcattctgt gattggcaga actaattgtt
      721 ctaaaacaag tttttctagt tctttgggat gataattgtt cagtgctttt acataaattt
      781 gtttcatgtt atcaaagttt tttgaatcta ctcccaataa ctgtcctaat tgaacgagtt
      841 cattgatcaa gcggaccgaa actacttccc gactcaaagg atttttattt tgtgcaaaca
      901 atgcatcaat caattcatgt actaaattct tcacttcctg atttccagaa cgaatggctt
      961 caaacaatcg attttgttct tctatcggat atctaataag tgttttttca tgagcagcag
     1021 gaacgatttc ctgataaaaa ataattgaac ttggaccagt atttacacga taatacaggg
     1081 cttcttttgc ccgatcaacc gattgcttgc tttcattcag cgtttcgaac cggttactga
     1141 tgcctatact gatagttaaa ttcaagtatt cttttacacg tttttgaatg agtcggcaat
     1201 actccatgac ttttttgctt gcttctgcat catctttatc cattcgataa atagtggctt
     1261 gcatttcttc attcaaaacg atcggcagca ttcgattttc ctttggcaca atttccgtga
     1321 tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc accgtgacta tcttctaaca
     1381 gatcgatttg aaccaaagct gtataatagc agtcattttg agaactataa ccaaattgct
     1441 gcaaccgctg atctagttct cgcctttcta ctcgatttct aaataaacta agtacaaata
     1501 gcgtttccag ctgtggcttt tgggtaacta agtgtgcact taacgcttct ttttctccta
     1561 tgatccgact gactgattga gcgaccaaat ctagttcttt cccactgaat ccttttgatt
     1621 taagattcag acgttcctgt atttgagaaa tcggacgggc aaatctgtcc gaaaataagt
     1681 aggaaagaag accaatgagt cctaccagtg ttaaagacac tgcaactagc ccgattctca
     1741 gattgcggat cattgcgcca attgcactag agtcaacttc taatctgtaa atccaattat
     1801 tataatctga tttcagtact atatacgttt tatctttttc tcttatcact ttcagctgat
     1861 tgtaatctag atccacgtcc gttaatacag ttggttgctc tttatttttt actgtctgag
     1921 tagtaaaaag gttgccaact tcgttttcaa tagaaagtat gtcttctcca ctttcaccaa
     1981 caacttgttc tattgtatgt ttctctatcg tagcaattcc caatgcatat ttttcttttt
     2041 caaacattgg caaagaaatc accatttcga ttcctttgtt tttcttttgc caaaataaat
     2101 tatttttgtt atttatgtac ttctgttgat acttttcaat ttcctcttcg gtcagttgac
     2161 tcaacgatcc attgattact ccccatttac cagccaaact gactaaatca taattattat
     2221 catccataac aatcgtttct ataaaattta gttctttcct gatttcagaa taagtggagt
     2281 aatctgtata tgtcagcgga tgcttgaata tgtccgagaa atttttggtc gaaccatacg
     2341 tagaaaacga atattcaatc gttctaattt tccattcaac gttagtttta acttgcctga
     2401 gcaaattctt tttgtctgtt aaataactcg attcgatcga ttcataggta ttctggtaaa
     2461 tgaataaact aaatcctatg acaagtacgg ctcctaataa aaacatcggt aaaaatattt
     2521 tataaaatac tgtacttttc ttcattttct tcctctcctc cttttaaaat aaaaggcttt
     2581 catttttctg taaaattgat actcatctaa ttaaatgagc attccccgct ttcaagaata
     2641 gcataattaa aaataaaaat ccaagtaatt tttaacgtgt gcaatctttt catatcaaag
     2701 atctattgac ttatcgtagt tatcgtaata tgatgaatgc gttaacaaaa agtgaaaaag
     2761 taaggaaaat gatggaatgg taacgatcaa agatgtcgct aaagcagctg gcgtttctca
     2821 tacgacagtt tctcgtgctt tgaataacag cagtttaata aaaaatgaaa caaaacaaaa
     2881 aatacgacaa ctagctaagg aaatgaatta ttcgccaaac tatagtgcaa aaggtttagt
     2941 gaatcaaaaa aaatatctaa tcggactatt tttctcaagt ttgcataaag gaacttcatc
     3001 aaatttccta gcagattcca ttgcaggcat ccattcagta ttagacacaa attacagttt
     3061 atctgttgaa agtatcgatg ttgttgattt aacagaaatc aacctgcaac gctacgatgg
     3121 actgatcgtt atgagtcaaa gtgatgatga tcagcctttc atcgactatc tcaaagaaaa
     3181 agaatttcct ttcgttgttg tcaatcgtca catcaatgat ccagcggtta tcaacgtcac
     3241 tgccgatgat gcggccggtg ttatgcaggc agtagattac ggtattggat gcggacatac
     3301 gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg acaaaagaac gggaaaaagg
     3361 ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat ccagcctatt ttttaacagg
     3421 agattacagc ttagaaagcg gcctccaaaa catggaatac ctattatcac tttctgacct
     3481 gcctactctg gttttttgcg gcaacgacga catagcgatt ggtgcattga gagctgtaca
     3541 tttagctggc ctccgtgttc cccagaacat ttctctgatc gggttcgatg attctccagt
     3601 cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag cctttgaaag aaatgtgtca
     3661 agaaggaaca aagcttttac tagatttgat cgaaggcagg acgatcgaac aatctaaaat
     3721 tgagctctcc accttgttga tccttcgtga gtcgatcaat tacttgtaaa ctacaagtaa
     3781 ttttatataa aaatgttaac gtgtgcataa cacagaaagg aagtttatat gaaaaaactg
     3841 acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag ttcttcagtt tggtgaaggc
     3901 aattttatgc gtggatttat tgattggcag attcagcagc tgaataacca acaattgttt
     3961 aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc ttggaaatat gatgaaccag
     4021 caagataatt tatatacggt cattttacaa ggactcagca atggcgaaat cgttgatact
     4081 tccgagatta tcaccgtagt agaacaaatg atcaatcctt atgatgaatg ggatgcttat
     4141 ctatctctag cagaaaatga cgacttggcc tatatcgtct ccaatacaac agaggcagga
     4201 atccaattcg tggagactga taatttaaca gatactcccc cacagagcct cccgggcaaa
     4261 ctgacagcct ttttataccg acgtttcaaa ttaaacaaag ctggctttac catcattcct
     4321 tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa tcattttacg ctacgcagaa
     4381 aaatgggatt tagaacctgc ttttgttgag tggttagaaa aagaaaatgt tttctgttgt
     4441 agtttagttg atcgcatcgt cccaggttat cctcgcgaag atgcaggtgt tttcaatcaa
     4501 cggcataact atgaagatca attgatggtc aaagcagaac cattcatgct ttgggtgatc
     4561 gaagggccta aagaattgga gaatcaacta ccattcaaac aggctggctt gaatgtcatc
     4621 atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc atttgcttaa tggaccgcat
     4681 acagcgatgg tccctcttgc tttgttggcg gaattggaaa ctgtagaaga agtaatgaaa
     4741 gatcctttat tctccgcata tgttgatcag ttattcaatc tagaattgat tcctatgctt
     4801 tctttaccta aagatgaatt agccatttat gctgaccaaa tcaaagaacg tttcttgaat
     4861 ccttttgccc atcacaaatt agaagcgatt tcattaaata gtgtttcaaa gttcagcacc
     4921 cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa atcaggtacc accgttaatc
     4981 actgtttcac tagctgcgct acttcttatg tatcgaggcg atcaagtaaa gccacatgat
     5041 gatgaaaaga cgataagcga atttactgat gcttggtctg ataatggaac agctattcct
     5101 cgattattgc aaaacgctgc gctttgggga gaagatttgt ctcaaatacc gaatgtgaca
     5161 gatacagttc aagaaatagc tgcaaagatc gatcaaaaag gtgtccggtc agtattacaa
     5221 gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa ccaacaatga aattaaatcg
     5281 acgagacagt gtggtcgtcg cattaacccc tattccaaaa caaacacaac tgacgattga
     5341 tcagcaaacg atcaccactc ttgaagatat cccgcaaggt cataagattg cattgggtga
     5401 tttgaaagct ggagacaacg tgatcaaata tggctatcca atcggtcatg tgacgacaga
     5461 cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa accaatcttt ctggtgaatt
     5521 ggattatcga tatgaaaaag atgtccatcc aagtcattac ctttttgaga accgaacatt
     5581 tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt aatgatttgt ttttagtacc
     5641 taccgttggg tgtgtgaatg gcattgctga actgatcgtc aaacagttca aggaaaaaca
     5701 tccagatctg ggacagtttg accatattac gattttaaag catccttatg gctgttcgca
     5761 gcttggaaaa gatcatcaaa atactcgaga aattttagcg gacgcagtaa atcatcccaa
     5821 tgccggagga gttttagtat ttggattagg ttgtgagaac aatacagttc ctgaattcaa
     5881 aaaaatttta ggtgaatatg atgaagaacg agtgaaattt ctagtggctc aagatgttta
     5941 cgatgagatc gaacaaggtg tggctctatt agaagagctt ttaacagcgg cggaaaatga
     6001 ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt ttgaaatgcg gcggttctga
     6061 tggtctatcg ggaataactg ctaatccttt acttggtgca ttttctgact acttgattgc
     6121 tcaaggaggc agcacgatct taacagaagt gccagaaatg tttggcgcag aacaagtatt
     6181 gatggcacga gcggaaaatg aagaagtttt tgactcaatc gttgacttga tcaatgactt
     6241 caaacagtat ttcttgtctt acggggaacc agtttacgaa aacccatctc ctggtaataa
     6301 agcaggtggg atcactacat tagaagataa gtctttaggc tgcacgcaaa aatcaggaag
     6361 ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc agaaaaccag ggcttagttt
     6421 attgcaagct cccgggaatg atcttgtagc tgcctctgct cttgcttcat ccgattgcca
     6481 gttggtcttg tttaccacag gaagagggac tccgtttggc tcatatgtcc caacggtcaa
     6541 ggtatctacg aatacaactc tttttgaacg caaaggtcat tggatggatt ttaatgcagg
     6601 cgtcttatta gaacaaccaa tggaagtaat cttagaatcg tttgttcaaa aaatcatcgc
     6661 tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac gttcgagaaa ttgccatttt
     6721 taaaaacgga gtaacacttt gattttcata aaacagtata aaaagaaaga aggaagattt
     6781 atgtttttat ctgatgattt tttattaaaa gacgattggg cgaaaaagct ttaccattcc
     6841 tacgcaaaaa acatgcccat tattgattat cactgtcact tagatccaaa agaaatttac
     6901 gaaaacaaca atttcagcaa tctgacagaa gcttggttag gaggcgatca ttacaaatgg
     6961 cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca caggaaatgc ttctgatttt
     7021 gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta tcggaaatcc gctttacagt
     7081 tggtcacatc ttgaattgaa gcgtttcttc ggtattgact tgttgataaa tgaagaaaat
     7141 gcagagaaaa tttggacaca agcaaatgaa attttggcag ctcctgattt tcgccgtcgg
     7201 gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa cagatgatcc cgcagacgac
     7261 ttgcactatc atcagttgct tgctcatgaa gaaccagatt tgaaggttct accttcattc
     7321 cgtccagata aagcactcaa tattgaaaaa gaaggattta atgcatgggt cacactactg
     7381 gaagaagcag ctgaccaaac gatcaccacc tatcaagaac tgatagaagt cttagggcag
     7441 cggatagaat acttccatca acatggatgt cgtgtttccg accacgcttt agacatctta
     7501 cgctataaag aagcggatga aacagtatta gaagagattt ttcaaaaagc acgagctaac
     7561 aaactattat cttcagatga aatcgatgct tatcgtacag aaacactgat acatttgacc
     7621 cacttctatc attctcataa ttggacgatg cagctgcaca tccatgctta tcgtaactgt
     7681 aatacacaaa tgttggaaaa attagggccc gataccggct atgatggaat gaatgatctt
     7741 tctttgacta ttcctctgca aaaattattg aaccgtgcag aagaaacgga tcagttgcca
     7801 aagacgatcc tttattcatt gaatccgaat gactatcctg cgattttagc tttgatgggc
     7861 tgtttccaaa aagaacagaa tggaaaactg cagttaggtt ctggatggtg gtacaacgat
     7921 actcgagctg gtatgcgcga tcaattgaca caatttgctg atggtagtgc tttggggaat
     7981 tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct acactagaca tgaatatttc
     8041 cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc gaggagaagc acctgaagat
     8101 gaacaacttt taggaagtct agttcaagcg attagttata caaatgctaa aaattacttt
     8161 ggcttttttg cctaattgat taatagtaac agtaacgaat caaaaacagg atagtgaaat
     8221 aacggaatcc gtttatccac gttttagaga agaagacgga ccaaatttgc tccgtcttct
     8281 tctcttgctg ttctgcttca ttgcctataa acctttatga aagaagagat aacatgccta
     8341 ctatccatat tgccggtgat tctactgcgg cagaaaaaga tgaaaacaag cgtcccgaga
     8401 caggttgggg agaaaaaatc tctgcttatt tttcacccga gatccagatc aataaccaag
     8461 caaagaatgg acgcagcacc aaatcattta tccaggaagg aaggttagcc cgtctagctt
     8521 cacagtttca gccgaatgat ttcttatttg tacaatttgg acataacgac cagaaaatcc
     8581 aagaagaaag aggtacgctt cccttcggta gctatttaaa aaatctgcag atattttttg
     8641 aatgcgcaaa aaaagcgaac gtccaaatgg tcttactgac atctgtgacg cgacgagatt
     8701 acttagaaaa cggaacattg aatccagata tacttggaga ctatccaaaa gcgatgcgtg
     8761 catttgctga gaaacaccat attcctcttt tagatgtgtt ctcaagatca caagaattgt
     8821 tccagacctt ctcaaaagaa gagacaaaaa aattctacct gcatcttata cctgatactt
     8881 gtaaaaatta cccagaagga ctaaaagaca atacccactt tagtccagaa ggcgctgata
     8941 aagttgcccg aataattgtt gaattgataa aagaatcccg tctgcctctt gctaattatt
     9001 tacaaaaagg agtttgagat gtttccatta aatttaggca tccgtgccca cgatttagat
     9061 gttcgaaatc gtgaagaaat cgttcaaaaa gtatcacagc ttcacttgtc ccatatccaa
     9121 tttgcaccac aaaaagcatt tcctgaacaa cttaaattaa ctgacatgac acttgggagt
     9181 gctgcctatt ttggtgaata cttccaaaaa aacaatatcg aactttccat tttaggatgc
     9241 tatatcaacc tatccagcaa aaatcaagaa gttcgtgcac aagcattggc aactttcaaa
     9301 cagcaaattg ctctgtgctc tgcctatcaa gccaagatga ttgctacgga aaccggtagt
     9361 gtctctgtcg gttacacaaa agaaaatttt actgaagaag cttatcagat cgccagaaat
     9421 tctatcattg aattagtcgc tcatgcagaa aattttggcg taactgttgc tgttgaagca
     9481 gggatcaatc atccaatcta taactatcag ttagctaaac gtttaatcga tgaagtagct
     9541 tcaccaaatt taaaaatcat tttagattgt gcgaatttga tgcataaaga aaatcatgct
     9601 gatcaggaaa aagtgatccg aggtgccttg gacaatttac atggttatat tacagcgctt
     9661 catctcaaag actatatcat ggaaaatgga aagatccgca tggtacctgt tggcaagggc
     9721 tgtttagatt tccgcccggt actgcattat atcaaatatg agcgtcctct aatgtacgcc
     9781 actcttgaag ctactcctga gatttatgtt cctgaagcaa tagaacactt gcagtttatt
     9841 taccaagatt gttaaggaga agattatgga ggaacgaata cagaaaagtt atcaaaaata
     9901 tcatgcaaat gcttttcaga cattgctgca tctttatcag ggaaattatt ggcgttttgc
     9961 catgtcagga ctctttttta ttatcaaaca tcttccatct tggttaatgc cgatcgctat
    10021 tgccaatgta atcaatgcag cttcatcgaa agattcttct cagttgcagg ccatttattt
    10081 caacgctttt ttgatggctt gtctgattgg tcaaaatata ttgacgaatt attttcatgt
    10141 aaaataccat agcacttcga tccgtcaagt agaagctgga ctaagagcga cccttatcaa
    10201 gaaaatccaa gaactatcta ttccttatca aaaagaattg caatctggac gaatccagtc
    10261 aaagatcatc cgtgatgtag aagctatcca aacgttgtcc tctcaagtat ttgtcagcgg
    10321 attaaatatc gctgtcaatt tattcgtagc tttaggtatc acgctttata aaagtcctgt
    10381 tgttttcatg tttttcttat tgactgttcc tctcgccgca ttcgttactc tctttttccg
    10441 taaaaggatc agacagtcca actcagactt caggcaagaa atggaagaga cttctgcaaa
    10501 agtgatcgaa gtgatcgaat tagcaccgat tgctcgagct catgggttag aaaaacacga
    10561 aacaactcgc ttaaataagc attttactca aatctatgaa aaaggattcc acttagatat
    10621 cctgcaatct gttttcggat cagtcagctg ggctagtttt cagatatttc agctgatttg
    10681 tttgattttc actgggatat tagctttaca agggcgcatc caacccggcg atgtcgtgat
    10741 gtatcagacg tatttcacga cgatcgtcaa ttccgtttct ggaatgatca cgctgattcc
    10801 tacgatttca aaaggcatgg aatctatcac ttctattggc gaactgttga ttgctggcga
    10861 tgtagaaact tatcacggta agaaaaaaat ccaacatttg gatggaaatt ttcgttttga
    10921 ggatgtttct tttctttatc cggactcact caaaccgatt atcgatcatt tttctctaga
    10981 ggtgaaagct ggagaaacaa ttgcttttgt tgggccatct ggctctggaa aatctacact
    11041 ccttaactta ttgattggat ttatccagcc gacttcagga aaaatttttc tagatgatca
    11101 gcctttggat gaactggata tgcgaagtgt gcgaaattat ttagccgttg ttcctcaaac
    11161 cactttgttg ttctccgctt cgatcaaaga gaacatcact tatggcttga aaaacgtatc
    11221 aaaagaacgt ttagatgaag tcatcgaagc tgcgcaactt tcttcactga ttgaccagct
    11281 tcctgatggt ctggatacct tagtaggcga acatggcaac aagctgtctg gtggccaaaa
    11341 gcaacgtatc tccattgcac gagcgttgat acgtcagcca aaaatcattt tgctagatga
    11401 agcaacctca gctttagata accagtcaga gaaaaaaatc cagcaagccc tgaattacct
    11461 gacagaaaca cccactactt ttattgtggc tcatcggctt tcaacgatca aagaggcaga
    11521 taaaatcgtt gtgatcgatg aaggaaaaat cgttgaaata ggtacttacg aaaaactgtt
    11581 agctcaaaaa ggctatttct accgcttgaa aaatgaatga caaaaaatag gagctatgac
    11641 aagacttttg tcgtagctcc tattt
//
LOCUS       DACEOE010000079        11569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEOE010000079 DACEOE010000000
VERSION     DACEOE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.081"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(157..2055)
                     /locus_tag="IW774_001713"
     CDS             complement(157..2055)
                     /locus_tag="IW774_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1154060.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2216..3478)
                     /locus_tag="IW774_001714"
     CDS             complement(2216..3478)
                     /locus_tag="IW774_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1154061.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(3471..4370)
                     /locus_tag="IW774_001715"
     CDS             complement(3471..4370)
                     /locus_tag="IW774_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154062.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(4515..6293)
                     /gene="uvrC"
                     /locus_tag="IW774_001716"
     CDS             complement(4515..6293)
                     /gene="uvrC"
                     /locus_tag="IW774_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1154063.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(6475..6789)
                     /gene="trxA"
                     /locus_tag="IW774_001717"
     CDS             complement(6475..6789)
                     /gene="trxA"
                     /locus_tag="IW774_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1154064.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(6876..9236)
                     /locus_tag="IW774_001718"
     CDS             complement(6876..9236)
                     /locus_tag="IW774_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1154065.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(9351..9899)
                     /locus_tag="IW774_001719"
     CDS             complement(9351..9899)
                     /locus_tag="IW774_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1154066.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(9957..10394)
                     /gene="zapA"
                     /locus_tag="IW774_001720"
     CDS             complement(9957..10394)
                     /gene="zapA"
                     /locus_tag="IW774_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1154067.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            10596..11513
                     /locus_tag="IW774_001721"
     CDS             10596..11513
                     /locus_tag="IW774_001721"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1154068.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
BASE COUNT         3267 a         2456 c         1964 g         3882 t
ORIGIN      
        1 gtgaaatcag cgccctgacc tgagtattag agaaataaac agaaaaatag cttctaatat
       61 tttaccgttt tttgagctaa ttccgaagga gactgcgaca cgccttttgt tgcagtcgct
      121 ttttagtcta ttgagcaatg ttggctagca tttttctcaa tcgtcctttc gataatccaa
      181 cagcctttgg agttgagtaa tctgttgctg taattccatt ccttttgtcg tattccgatt
      241 aagtttgcgt gggagctgct gctcgatcac tttttttaca ttcgtttgat cagatttttt
      301 tgcaaattgt tgttcaatag gcaagaacgg ataatgtgtc acgatttgga aaccgtaaga
      361 attgcatagt aatgagtagc cagcaatccc ggttgttttt tgataagctt tggatagacc
      421 tccgtcaata acaaatgctt ttccgtttgc tttgatcggt gactctcctt tttttacttt
      481 tactggcgtg tgtccattga tgattgctga accttcttct tttgcatcga attctgccaa
      541 aattttttca ctgaaccact cttcatctcg caagtgataa tagggattgc ttttttcgac
      601 gtgcgtatga ggctctgtga gaaaatagcg ctcaaaagtt gccattttac tcttgccgaa
      661 taaaggcgaa aatggcccgc tccacgcata ccaaatcaaa tcggtcgcca aatcttcatt
      721 tgcagtcaaa tcttttgcgc tttcagttat ctgtttttca aaaaatccca gcagttcttt
      781 tccgcgataa gattggtcta attcaaatgc taagagctgc cccttttcat ctacaggtat
      841 acagccatgg aacagtacat gctgattgtt cgtgagatac attccccctt tttcgatcaa
      901 aaacgtcatg tgtttttgca ttcttaacga gtgctggaat gaatacatca aactatcgat
      961 cactttttgt tcctcttcca agaatttttt aggatcatct ggctggatcg tttgaaaaca
     1021 agtccctttc aactcataga gctgaccttc taaaacgatc gttgattcct catagttgat
     1081 tttttccaac aatttacgat cgtccatttt aaattcaggt cgtcgattca acaactgatg
     1141 ttctgatttg aattggagga tagaaagtgc ttgatggatc ttttctaatt cttcttgctc
     1201 tctttcagat agatctttcc tgtttttagg ggtaaatact ggattcttct gataggtttg
     1261 atgggcaaaa gaaaataatg gacgtaaatt taatccatat tctttctcta attcaaataa
     1321 atagttgtag cgtgcggcaa tccgtaaaag cgtcaataaa catgcctcag agccgcaata
     1381 cgcaccaatc cacaaaatat catgattccc ccactggata tctacagagg aaaaatccat
     1441 gattcgatcc attactttgg ctgcttgagt tcctcgatca aaaatatcac ccacgatatg
     1501 caaatgatcg atgacagatt tttggatcgt ctgcgcaagt tttgttaaaa atttttcttc
     1561 ttccttcatc aagacaagtt gttcaatgat tttttttaca tagtcttttt tcccgttgat
     1621 acgatcatcg atatacaaga gttcctcaat gatatagcta tattctttcg gcaatccttt
     1681 tcgaactttt gatcgagtgt atttgactgt cgtaaaccgt aaaagatcga tcaaacgatg
     1741 gatcagctgg gatttatctt gttttttgta ccaatcagtc gtcaacacat attctggata
     1801 agccgtaaaa atagtgaatt ggctgatctc cgtttccgtc atttgttcag agaaaaggtc
     1861 tttgatcttt tctttaagat ttcctgaacc gatacgcaga atatgatcaa aagcaggaaa
     1921 ttcgccatgt atatcgctca aaaataactc tgttcctttt ggaagctgca aaattgcttc
     1981 gagattgatg atttccgtga tgacatcttc cgttgtaggg aacttctctg aaagtaaatc
     2041 taaatagcgg tccattaaaa cgcctccaaa aattggtata ctcaacatta taaagttgtt
     2101 atgctctatt ttcaatgaat atatatccaa tataataaaa aaatagaaat aggtctatac
     2161 aaaaaagaag ctgggaacaa taaatgtcca gcttccttct atatttttct atattttatg
     2221 ctttttttcg ttcatttctc ataattgaaa tcgattgttt caatgaactc cataatccgc
     2281 cttcgctata cacgagtaca ttcgatttgt acatgtttgc agacaagatc atcaataaga
     2341 atgttacggc tacaagtacc aacaaggaaa tcaatgctcc acccataccg actgtctcat
     2401 ttgctaagcg aaccggcata atatatgaag ataagaatgg aatatacgaa gtgatccgaa
     2461 taacgatatt gtttggatct gaagctccta gtatcaatcc aagcatatat ccgcctagag
     2521 atagataagt cactggcaaa attgcttttg ctgtatcttc tgctttgttg accaaagagc
     2581 cacataaagc tgctaaaaca gaaaaaatca aaatgccaaa gatcatgaat agaagagaat
     2641 accacaagaa tggtccaatc aagttatcca atgagatacc gtctaatata tttttgacca
     2701 tttccatatc tttgaattga ctgtatccta taccaaaaat aataccgtat aatgccattt
     2761 gcgttaatgc aacaagtaaa acacctgtta gttttccgta aaaatgcttt tgtgcagtcg
     2821 tactggacag gatcacttcc atgatccgcg tgcctttttc agaagcgatc tcttgtgcaa
     2881 tgatctgcgc atacgtcaaa ataataatga acaagacaat cgttgctaca taacttacga
     2941 catattggac tgtcgagtta tcttctccaa ctgtcatttt tccttttgaa tcaaaattga
     3001 ctttttgtcg agtcaaactt gcttgttggc tcaagctagc tacttcttct ggtgaaatgc
     3061 ctaattgact tgctcgaagc atcgattgca agccagtcag ttgttgttgt atcagtagtt
     3121 gtgtactttg tcctaatgaa ttttcgctat aaagcatccc atttatctta tcattctcag
     3181 ttgagactac taaatacgca tcgatttttt cttccgaaag ctctttttgt gctttttctt
     3241 cagaatcaac tgctttgaac tcaaaatcat ctgttttgac ttggctcatt tgctgactga
     3301 tttgctcatt atcagccaat acgccgattt tattgacatc actattttgt tgagcaaagt
     3361 ttcctgctac ataaatgata cccagcatga taaaagggac taatatcatg atcaagaaag
     3421 aaatcgactt aacatttttt aaatagacat ctttggtaat aatccaaaac ttacgcatcg
     3481 atttcacctg ctttcaattt gaaaatctct tccaaagttg gcggttgctg gttaaacata
     3541 ggaatgtagc cgaactgagt cgcacgagta aagatttctt tcccagcttc aggatcttct
     3601 aaggtaatct ctaacgtatt gtcttcatga aggatcactt ttttcacgcc ttcgatttct
     3661 tctacttcct gttggcttaa accagattcc aagaataact tcgtacggcc aaatgattca
     3721 cgaatctcat gtacttttcc attcaaaacc atttttccgt ttcgcaacat gatcaaatga
     3781 tcgcagattt tttctacatt atccatattg tggctagaaa aaatgacaca tgaaccatgt
     3841 tttttcaatt ccaatattcc atcttttagc aattcagcat ttaccggatc taacccgctg
     3901 aatggctcat ccaaaatgat caatttaggt tcatggatta acgtggcgat cagctggact
     3961 ttttgctgat ttcctttaga caaagatttt actttatccg tctttttccc ttttacctga
     4021 aatttttcca tccaaaaatc gatttttggt tcaatctctt ttcttgattt ccctctaagt
     4081 tctgcaaaat aaagcagttg gtcttgaatc gttactttcg gataaagtcc ccgttcctcc
     4141 ggaagataac cgatgacatc ataatctttc tccccaagat gatggccatt ccataacact
     4201 tctccttggt cttgtgtcaa aaaatccaag atcaaacgaa aagtcgttgt cttcccagcc
     4261 ccgttttggc caattagccc catgattttt ccatcgggga tttggaaaga gacatgatcg
     4321 acagctgtgt agttcccaaa agttttaaca agatccttga cttctaacat ttcttcactc
     4381 cttcttcaag aaatcacata tatccttaga caccagacgt aagcgattta ttatttacca
     4441 acaaaacgta aacgctcttt ttgttgtaca taaaatgata ggacacttta ctttctttgt
     4501 aaagtgccct ttcctcattc ctgatgtaat ttatcaaata cgttttgtgc gacatttttc
     4561 ggcagtccga tttcttgtaa ttcttctgtc gtggctgctg tgatattttt caacgatttg
     4621 aactctttaa gcagcatttt ttttcgtttt ggtcctaacc cttcaatttc atctaatcgg
     4681 gaagcaaaac tgtttttact gcgtagctgc cgatggaaag tgattgcgaa tcgatggact
     4741 tcatcttgga ttcgctggag taagaaaaac tctggtgaat ttcgctcaag cggtactaca
     4801 gccaaatcta taccgaatag caactcattt gttttatgtt tatcgttttt tgccatgcca
     4861 gcaataggaa tatcgattcc taattgattt tctaagacat cttttgccac atctacttgt
     4921 ccttttcctc catcgatcaa gatcaaatca ggtaaaggca acccttcgcg aatcactcgt
     4981 gaatatctgc gatagatgac ttctctcatt gatgcataat catccggtcc gttgactgtt
     5041 tttattttat atttccgata ttcttttctt gccggttttc catcaatgaa tacgaccatc
     5101 gcagccacag gattcgtccc catgatatta gagttatcaa aagactcgat acgtattggg
     5161 gtcgggatat tcatcgcgtt tcctaaacgt tcaactgctc cgatcgtccg ttcctgtttt
     5221 ctggcaatca gatcaaaccg ttcattcaaa gcaactgagg cattcttatt cgccaattca
     5281 actaattttt tcttttctcc tcgttttggt tgcagcactt tcgtagctaa caaagcttct
     5341 acactgggct tgtcgatcgt atcaggaatc aagacttctt taggaatgaa atgctcattt
     5401 tcctgataaa actgaccgag gtaagtcaag aaatcttctt ctgcttcgtt atagaaagga
     5461 aacattgaaa catctcgttc aattagcttt ccttgtctta cgaaaaacac ttggacacac
     5521 atccatcctt tgtcaatcgc ataaccaaat acatctcgat ccagtaaatc agtatttgtc
     5581 attttttggc gagtcatgat tgtctcaatg gcacggatct ggtctcggta ttctgcagct
     5641 ttttcaaact ccatgttttc agcagctttt tccattttct gatggatctc tgactcgatt
     5701 ttttcgtgtc ctccgtttaa aaactttttc acttcatcaa cgattttcgt atagactttc
     5761 ggatctacgt cgaaataata aggacacaga cattgtccca aatgatagta aagacaaggc
     5821 tccttcgtct gactaggttt gcattttctc agaggaaata atcgatcgag gatcttcttt
     5881 gtctcattcg ccgcaccgac atcgggatat ggtccaaaat acagtgcttt gtcttttagt
     5941 actttacgag taatcaccaa acggggatat ttttcattcg ttatcttaag gaaaggatag
     6001 gttttatcat cctttaacat aatgttgtat ttaggatcat ttttctttat cagattgatc
     6061 tctaagagca atgcttcgat attcgattct gtaacgatgt attcaaagtc ttcgatttca
     6121 ctaacaagcc gttccgtttt cgtattatgg ctgccagtaa aatatgagcg gacacgattt
     6181 tttaatattt tggctttgcc gacataaatg atcgttccat ttttatcttt catcaagtaa
     6241 cagccgggct gatcaggaag taaagctaat ttgtttttga ttcgttcgtt catctggtac
     6301 tccccttcat attcaattaa agttattata gcatgtctgt caaaaaaaca atccagcctg
     6361 acaacgtaat tatactcaaa aaatattaat ttgaataacc ttaactcaac cgcaaaaaaa
     6421 ccgaggacgg aactggattc agtcccaccc tcagcttatt tttgctgtaa gtttttataa
     6481 atattgatcg atcatttgac gtagttgttc ttttgaatga acaccaacag ctttttctac
     6541 tacttgacca tcttttttca acattaatgt tggaatgctc atgataccga attgttgagg
     6601 agtttctgga ttttcatcca catccatttt agcaattctg aattcttctt catcatattc
     6661 ttgttccaac tgatccaaaa tcggtgcttg catacgacat ggaccacacc aagttgccca
     6721 aaaatcaatc aatacaagtc ctttatctgt ttcttcatta aatgtcttat ctgtaattac
     6781 ttgtgacata tcaaagacct ccatttttaa aatctatgat tagtatagca tttctatact
     6841 tggatagcga accatctgct tacctttagt aagctttact taaatttaac aattgtcgca
     6901 ccatttccgc cttggtttgc cggtgcaaac tcaaagcttt tgacgctccg atggttttta
     6961 agataatcag taattccctt gcgaagtgcg cctgttcctt ttccgtggac aatagtcact
     7021 tgaggataac cagctagaat ggcagaatcc aaatattgat ctacctcact tagcgcttct
     7081 tcgtatcgtt ttcctcgaag gtccaattga ttcggcacgt gactactaga ttcagatcgt
     7141 acagcatgga taactcgttg tgtcggctct ttttgaggag caacaggcgt cagctcgtct
     7201 tcggacacat tcatttttaa tataccgatt tctacttgcc actgattccc gtttttacgt
     7261 aaaagcgtcc ctctttggcc gtaagtcgta accaacactt cgtctcccgg tttcaacgtt
     7321 ttttgttctt tcgccttctt caatacttta tttttcttca aagtttcttc ttgatgtaga
     7381 tttgctaatt gggtctttgc atcgatcaac tgatgctctt tcacatttcc ttgcccaatc
     7441 tgctgttgca ttttacgaat atcagcaatg attttttctg ctttttcttc cgcctctgac
     7501 acgacttcgt ttgctttttt ctttgctttt tccatttctt tttcacgttc ttcaaagaaa
     7561 tagctgtaag cttctttcaa atcatcgtga agttcttgtg cctcagaaac aaaatgacgc
     7621 atttccagat attcggtctc tgccattttc cggcgattct ctaaatctgt gatcatttcg
     7681 tttaaatctt ggctctcatc attcatcaac tgcttcgctt catcaataac ttctgtatca
     7741 agacctaatc ggctagagat ttcaaacgca ttgcttcgtc caggaacgcc aatcagcaaa
     7801 cgatacgttg gacttagtgt atccacatcg aactccatgc tggcattaat cgtatttgcc
     7861 cgattgtatc cataaacttt caattcagga taatgcgttg ttgccatcac ataagcagac
     7921 tttttaccaa gatcatctaa gattgcgatt gctagagcag caccttcttg cggatctgtt
     7981 cctgcaccga gctcgtcaaa taataccaga cttttttcat tgacttttga taaaatatct
     8041 acagtgttcg tcatatgaga agaaaaggtc gatagacttt gctcaatcga ctgttcgtct
     8101 cctatatccg caaatacttc ctcaaaaata ccgatttgac tttcttcacc tgcaggaatc
     8161 ggtaatcctg cttgccccat cagttgtaac agacctaaag tcttcaacgt gatcgttttc
     8221 ccacctgtgt taggtcctgt aatgacgatg gcttgatagt cttttccgat tgtgatgtca
     8281 ttagaaacta ctttttcctg atcgatcagt ggatgtcttg cttgtttcaa gacgacatga
     8341 ttgtcttcac tgattcccgg cacgatggct tttacttctt taccaaatcg tgcttttgca
     8401 ttcatcagat ccattttacc aatcacataa gcattgtgga tgatttcttg acgatgggga
     8461 acaagttcag cagaaagttc acttaaaata cgttgaatct catttcgttc agcgatttgg
     8521 tattgacgca aacgattatt caaatccacc acttgttttg gttcaataaa taaagtctgt
     8581 cctgaagcac tttgatcgtg gactacaccg ccaaatacac cgcggtattc ttgtttgact
     8641 gggataacgt agcggtcatt tcgcatggtg atgatcgcat cacttaaata ctttgcattt
     8701 ttcccacgga cgatcccatc taactgttca cgaacagctt gttcacttcg ccgaatgttc
     8761 tgtcttatgc tctttaactc aggagaagcg tcatcagtca cgcgtccgtc ttcatcgatt
     8821 gcttccttta aacggcggct caaaacaggg atcgtgacta actgatctgc ccacatatac
     8881 aaacgtgcaa attcgatttc actgtcagaa agatcatcaa tgaaacgatt caattcgctt
     8941 gtcgtagaca atactctgga aacttgtgca agctccactc cattcaagtc tgctccgatc
     9001 tcgatccgtt tcatatgggg acggatattt tcaagtttag gtatagggat cccgcctcgc
     9061 agacgttgca ctttcaatcc atcctcagtt tcttgaagcc agtttgtgat cgtttgtttc
     9121 tcactcactg gtaccaattc tgccagttct tcttttcctt gtgcagtcac tacaaattgt
     9181 ctgaccatct gtttgacttt ttcaaattcc aatgtttcaa ggattcggtt attcatttta
     9241 tcaccttatc tttcactatg aaaaaagagc tgtgccggac atacgctacg cagccttctt
     9301 aaataaacta ctctgtacaa aaaacgaaat attttccagc actattttcc ttaattcaaa
     9361 atattcgtta cccaaagctc atacatcttg ttggtaagaa atggcgttct ctcaacgata
     9421 aaacgagcga aactatgctc gccgaattga ttttggacca catctaatgg aacaaaggca
     9481 agtaactgca aaatcaaaaa tatagtaacg taagccatga ttagtgacag gatgcctcct
     9541 gcaatccagt ctacctgtct caagatcggc acatacgtca atccatggac aaaaaccccc
     9601 acaaatcggg taagcagcca gccaattagc aaaataatca gaaaagcagt tcctgcataa
     9661 aaagcttcat ctaggtgaaa agcaaatacc tgatcaaaaa atgctaactt tgatgttggg
     9721 gtgacagctg ggtatggaat gtataattcc aaatgacttg ctaatggttt gtaaaaatgc
     9781 tgggcagcaa taaatgacaa tacatatcca atcgcgaata tgatttgcaa agcaaacccg
     9841 cgacgtgctc cagaaaaaaa cgcaattagc aataaaaata aaatcagcaa actcagcatg
     9901 ctacgttcct ttctcttcta acttttttaa atttttctta ttttgataaa aggatcttaa
     9961 ttagaagatt tttgtttaat ttgtcctttt cgtttctcgt ttaaaatctg ttgtgcttcc
    10021 acatgatttt taatggtatg atctgcttga ccattttctt tcaatacttc ccgagcttcg
    10081 ttctcgattg cttcaatgcg tttcacacga ttttcaagct ctgtaaattt gatcattttt
    10141 ttctttaatt ctgctgtttc ttcttccagc tccaagattc tttcttgttt cttcaattga
    10201 tccgaaagcg cattgacagc catcaagatt gctgcttgtt cattatccat ttgtggtgat
    10261 aattgtttca attctgctaa ctgttcattg atcagcttcg ttacgatatc catatgatgc
    10321 ttggtttccc gtccgataat agtatatgtt tggttggcaa tcactgcttt gtagcgtgtt
    10381 ttttcgtgcg ccatacgaat catttcctcc tatttgatga aagttttatt tagtgtacca
    10441 tgaagttggt tctttcaccg ttctccacaa atatttttca catacttaac tattatatga
    10501 taaactttaa aaaaatgcta tgtctcgcaa tctcttagaa aacgaaaagt cgtgagttgc
    10561 ggtatagttt tatgtataaa aacgaggaga attttatgag caacgtcatt atcaaagcaa
    10621 caaacgacca acttagtcaa atgaaagcat actatcgctc ttatttgtta aataaacaga
    10681 ttccttatac tagctttgct gcaaaaaaag acgggacgac cattaccgct tatacatccg
    10741 gaaaagtgat gtttcaaggt acgaatgcag caatagaagc tgataaatgg ggggaggcac
    10801 aggataaaca agccaagcca gcaaaaacag cctcttcttt gccaaaaaat atcagtcaat
    10861 tatccgtttt aggtagtgat gaggtcggaa atggcagcta ttttggtccg ctaacagtat
    10921 gtgctgctta tgtgaaacgt gatcagcttg cagaattacg taaacttggt gttagagatt
    10981 caaaagagtt aaaagatagc caaatcatcc agcttgctga catcttaaaa aaaaccatcc
    11041 cctataaact gcttgtctta tctccagaaa aatacaatga gatccaacca aaatacaatg
    11101 ccgttcggat gaaagtcgct ttgcacaatc aagcgatcca tttgctgcta aaaaaaatcg
    11161 agccagaaaa accagaagcg attttgatcg atcagtttac gccggaagca aattttaaaa
    11221 aatatgcgcg tttagaaaaa aatcaattac aagaaaaact gtacttcgtt actaaaggag
    11281 aacaatatca tttagctgtt gctgctgctt ctatcatcag tcgtgccagc tttttggaag
    11341 agttacacaa agcatcagct gaagctggaa tcacccttcc atcaggagct ggaacgaaat
    11401 ctgaccaagt agcggcaaaa ctattggaga aaggcggcat gcctatgctt gaaaaatatg
    11461 ccaaacttca ttttgccaat acagaaaaag ctttgaagct gctaaaaaaa taatggaact
    11521 gtgacaaaag tcatgtcaca gtcccttgtt ccgaataaac ggtgttctc
//
LOCUS       DACEOE010000080        11244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEOE010000080 DACEOE010000000
VERSION     DACEOE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.082"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(7..1236)
                     /gene="hflX"
                     /locus_tag="IW774_001722"
     CDS             complement(7..1236)
                     /gene="hflX"
                     /locus_tag="IW774_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1154069.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="IW774_001723"
     CDS             complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="IW774_001723"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1154070.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2159..2896)
                     /locus_tag="IW774_001724"
     CDS             complement(2159..2896)
                     /locus_tag="IW774_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1154071.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAENPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2987..3514)
                     /locus_tag="IW774_001725"
     CDS             complement(2987..3514)
                     /locus_tag="IW774_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1154072.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3694..4287
                     /gene="clpP"
                     /locus_tag="IW774_001726"
     CDS             3694..4287
                     /gene="clpP"
                     /locus_tag="IW774_001726"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1154073.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4391..4876)
                     /locus_tag="IW774_001727"
     CDS             complement(4391..4876)
                     /locus_tag="IW774_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1154074.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5029..5226)
                     /locus_tag="IW774_001728"
     CDS             complement(5029..5226)
                     /locus_tag="IW774_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154075.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="IW774_001729"
     CDS             complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="IW774_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1154076.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="IW774_001730"
     CDS             complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="IW774_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1154077.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9201..9413
                     /locus_tag="IW774_001731"
     CDS             9201..9413
                     /locus_tag="IW774_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1154078.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9415..11094
                     /locus_tag="IW774_001732"
     CDS             9415..11094
                     /locus_tag="IW774_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1154079.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3258 a         2429 c         1970 g         3587 t
ORIGIN      
        1 aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc ctttaacttc
       61 atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc ttctcaactc
      121 gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca ttagttccat
      181 cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct gagtagaaat
      241 caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt ctgccttatt
      301 gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt ttaagacagt
      361 ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca aaagatccat
      421 attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag gcaggtcttg
      481 gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac gccatctctt
      541 agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg cttgtgtcaa
      601 tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa tctgaaaaat
      661 ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt ctttcaattc
      721 tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta actttgtttc
      781 acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc cttgccctac
      841 taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc cttctttcga
      901 acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta ccggaagacc
      961 aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa agatgatcaa
     1021 atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt taccgataat
     1081 cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt ctcctgatgc
     1141 ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct ggtttgcttc
     1201 ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc agccattttt
     1261 tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca aaccactgtg
     1321 catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc gaatgtagtt
     1381 tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg aaaaattctt
     1441 tatatccaat accttgactg gcctggactt ctccaagctc atacacctta cgcgcttctg
     1501 ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca taaagaatac
     1561 tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc tcttctttag
     1621 gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc actttgcgtt
     1681 gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac tgattccata
     1741 atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc tcaggattcg
     1801 cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag agacccgtac
     1861 caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat tgtcgacctg
     1921 aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca atcaaatgat
     1981 gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc agatttttat
     2041 agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg agttccacac
     2101 ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt tccatatttt
     2161 acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca gttattattc
     2221 catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc gtccatgcac
     2281 gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa gtattttttg
     2341 gatgtactcc ttctatataa gggttttctg ctgctagttg gatcttttta tcagaagtac
     2401 tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc aaagaatcca
     2461 aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat gtttcggcta
     2521 atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc attagcatcc
     2581 ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga actttagctg
     2641 ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct tccaatgttt
     2701 tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg tgcataacga
     2761 tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca cctactcgta
     2821 cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt ccgcgatggg
     2881 catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt aagaaaaaaa
     2941 gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag ctccaaacat
     3001 catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga gctttttttt
     3061 cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt ccgcctttat
     3121 aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt cgatgggaga
     3181 tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt tgcgctgaaa
     3241 atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat gcatcaaagc
     3301 tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc tttttattta
     3361 attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta cgctgttctt
     3421 tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca gcacctgtca
     3481 gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt gttttgtttc
     3541 tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt ttttatttga
     3601 ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat tatggtacac
     3661 tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac agtcattgaa
     3721 caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa agaccgtatc
     3781 atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc ccaattgctt
     3841 ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc tcctggtggt
     3901 tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc agatgtccaa
     3961 acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc tggtacaaaa
     4021 ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct tggcggcgct
     4081 caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac acgcgaacgc
     4141 ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga aaaagataca
     4201 gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat cgatgaagtc
     4261 atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt aagctctctt
     4321 atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt tattttatgg
     4381 atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga cctatctcga
     4441 cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa ggacggatca
     4501 ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct tctgttacta
     4561 atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat atcgtggtcc
     4621 ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac tgatgtccta
     4681 ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg attgtaccat
     4741 cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt aaaacagctt
     4801 gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg gcatttgatg
     4861 ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact aatcctattg
     4921 aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa aacctgctca
     4981 ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt attgtacatc
     5041 tatattttct tctattactt tttttaaaat taattcgtac acttctattt gtgctgccaa
     5101 gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct tcgtctgaca
     5161 gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat ttaaaaactg
     5221 ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc aagaatagag
     5281 aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct tcctcagcaa
     5341 attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc tcatgcgttc
     5401 ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca cggattgtcg
     5461 ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc attgcttttt
     5521 gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa atcaagattt
     5581 taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct aaagacacat
     5641 tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg ataaaatgat
     5701 ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca agctttccga
     5761 agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag accataccaa
     5821 atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca atccttattg
     5881 ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc ttgccggcac
     5941 cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc aaatcttgga
     6001 ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca tctcctaaaa
     6061 tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca tctaaaatct
     6121 cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat tgtgtcacgt
     6181 tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt tgaccgacaa
     6241 cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata taagtagcat
     6301 aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca cgaaacccaa
     6361 agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt tcgcgtccat
     6421 atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt agctcaccaa
     6481 gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt tttgagatcc
     6541 agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg atataaaaca
     6601 tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata aaagcttgtt
     6661 gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata tttccttgct
     6721 ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg tctcttctca
     6781 aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt aattgcgaaa
     6841 tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata taagaaaaat
     6901 tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt cctgtaacat
     6961 atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact aaaagaaaag
     7021 tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag gtttttttca
     7081 tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg ggcatttttc
     7141 caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata cgatgatctt
     7201 atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag attctgtcgt
     7261 ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg cagaaaagct
     7321 gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg ccaatcgttg
     7381 tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa atccatctgg
     7441 ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca tttcttcttg
     7501 cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa atagcagcgc
     7561 tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat ctctcacgtc
     7621 tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga tcaattggat
     7681 caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc ctggagaggc
     7741 tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag caaatgttac
     7801 attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg gatcttctac
     7861 aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag ctgctcttgg
     7921 atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga atatatactc
     7981 gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca ctgccccgct
     8041 ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt gtgcaacagc
     8101 catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta cagcaaaacg
     8161 tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg aaagattttc
     8221 ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata acggttcaga
     8281 caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta ctacatacaa
     8341 actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca tcattggtgc
     8401 cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag catcgtttgt
     8461 tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg aataactttg
     8521 gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa tcctcgaacc
     8581 aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga tcataaatat
     8641 cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga tcatacgtgc
     8701 taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata tgaaattgaa
     8761 aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt tctcatctcc
     8821 ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc ctattaatag
     8881 aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca ttgatttttt
     8941 ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata acaattaatt
     9001 gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga taagcaaaaa
     9061 cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga aaaaccaggt
     9121 ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca ctgctttacg
     9181 cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca aaagtacgca
     9241 accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat gtagacgctc
     9301 gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt ctagaaggaa
     9361 gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt taatatgaaa
     9421 ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg tgaaatcggg
     9481 aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc cgggatcaaa
     9541 ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag ctacatcata
     9601 caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga ccacatcggc
     9661 ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc tctcgcatta
     9721 gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga gctccatgag
     9781 atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt cagaacgacc
     9841 catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa tatcgtagcg
     9901 actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt gcataaaatg
     9961 gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa tgctgagatt
    10021 ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat cttttcaaaa
    10081 atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt gcagcaagca
    10141 gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag catggaaaat
    10201 gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac atttgtagat
    10261 gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac aggttctcaa
    10321 ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca aatccaaatc
    10381 cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac aacaagtgtc
    10441 aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg aaaaatcaat
    10501 aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct tcgtttgatg
    10561 aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt acatgcttct
    10621 ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa tggcgatgtt
    10681 ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg agatgtgtat
    10741 atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg tcacctgctt
    10801 tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa agaaatcttg
    10861 gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg agaaatgatc
    10921 aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg acctgactgt
    10981 tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt attcgaacgt
    11041 acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga ataaataaga
    11101 ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa acatgagaag
    11161 ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc tcttaaattt
    11221 atttactttt tcatacaaaa aaat
//
LOCUS       DACEOE010000081        11242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEOE010000081 DACEOE010000000
VERSION     DACEOE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.083"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(80..799)
                     /locus_tag="IW774_001733"
     CDS             complement(80..799)
                     /locus_tag="IW774_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1154080.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(848..2494)
                     /locus_tag="IW774_001734"
     CDS             complement(848..2494)
                     /locus_tag="IW774_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1154081.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2673..3263
                     /locus_tag="IW774_001735"
     CDS             2673..3263
                     /locus_tag="IW774_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1154082.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3355..4860
                     /locus_tag="IW774_001736"
     CDS             3355..4860
                     /locus_tag="IW774_001736"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1154083.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(4944..6296)
                     /gene="celB"
                     /locus_tag="IW774_001737"
     CDS             complement(4944..6296)
                     /gene="celB"
                     /locus_tag="IW774_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1154084.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6296..6814)
                     /locus_tag="IW774_001738"
     CDS             complement(6296..6814)
                     /locus_tag="IW774_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154085.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(6830..7156)
                     /locus_tag="IW774_001739"
     CDS             complement(6830..7156)
                     /locus_tag="IW774_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1154086.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7169..9148)
                     /locus_tag="IW774_001740"
     CDS             complement(7169..9148)
                     /locus_tag="IW774_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1154087.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9169..9483)
                     /locus_tag="IW774_001741"
     CDS             complement(9169..9483)
                     /locus_tag="IW774_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1154088.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9651..9944
                     /locus_tag="IW774_001742"
     CDS             9651..9944
                     /locus_tag="IW774_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1154089.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10022..11134)
                     /locus_tag="IW774_001743"
     CDS             complement(10022..11134)
                     /locus_tag="IW774_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1154090.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
BASE COUNT         3549 a         2285 c         1908 g         3500 t
ORIGIN      
        1 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
       61 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      121 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      181 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      241 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      301 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      361 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      421 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      481 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      541 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      601 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      661 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      721 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      781 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
      841 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
      901 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
      961 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1021 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1081 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1141 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1201 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1261 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1321 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1381 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1441 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1501 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1561 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1621 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1681 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1741 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1801 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     1861 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     1921 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     1981 gcgacagcgc ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca
     2041 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2101 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2161 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2221 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2281 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2341 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2401 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2461 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2521 cattactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2581 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2641 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2701 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2761 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     2821 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     2881 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     2941 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3001 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3061 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3121 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3181 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3241 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3301 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3361 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3421 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3481 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3541 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3601 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3661 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3721 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3781 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     3841 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     3901 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     3961 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4021 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4081 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4141 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4201 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4261 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4321 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4381 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4441 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4501 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4561 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4621 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4681 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4741 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4801 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     4861 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     4921 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     4981 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5041 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5101 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5161 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5221 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5281 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5341 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5401 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5461 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5521 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5581 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5641 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5701 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5761 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     5821 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     5881 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     5941 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6001 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6061 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6121 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6181 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6241 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6301 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6361 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6421 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6481 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6541 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6601 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6661 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6721 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6781 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     6841 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     6901 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     6961 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7021 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7081 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7141 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7201 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7261 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7321 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7381 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7441 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7501 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7561 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7621 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7681 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7741 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7801 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     7861 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     7921 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     7981 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8041 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8101 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8161 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8221 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8281 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8341 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8401 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8461 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8521 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8581 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8641 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8701 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8761 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     8821 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     8881 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     8941 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9001 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9061 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9121 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9181 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9241 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9301 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9361 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9421 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9481 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9541 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9601 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9661 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9721 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9781 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
     9841 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
     9901 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
     9961 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10021 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10081 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10141 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10201 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10261 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10321 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10381 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10441 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10501 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10561 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10621 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10681 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10741 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10801 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    10861 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    10921 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    10981 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11041 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11101 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11161 acatcatatg gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa
    11221 aaacatatcg ttttcttgta tg
//
LOCUS       DACEOE010000082        11029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEOE010000082 DACEOE010000000
VERSION     DACEOE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.084"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(90..965)
                     /locus_tag="IW774_001744"
     CDS             complement(90..965)
                     /locus_tag="IW774_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1154091.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1047..1838)
                     /gene="codY"
                     /locus_tag="IW774_001745"
     CDS             complement(1047..1838)
                     /gene="codY"
                     /locus_tag="IW774_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1154092.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1856..3256)
                     /gene="hslU"
                     /locus_tag="IW774_001746"
     CDS             complement(1856..3256)
                     /gene="hslU"
                     /locus_tag="IW774_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1154093.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3270..3818)
                     /gene="hslV"
                     /locus_tag="IW774_001747"
     CDS             complement(3270..3818)
                     /gene="hslV"
                     /locus_tag="IW774_001747"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1154094.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3855..4760)
                     /gene="xerC"
                     /locus_tag="IW774_001748"
     CDS             complement(3855..4760)
                     /gene="xerC"
                     /locus_tag="IW774_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1154095.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4958..6334)
                     /gene="trmFO"
                     /locus_tag="IW774_001749"
     CDS             complement(4958..6334)
                     /gene="trmFO"
                     /locus_tag="IW774_001749"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1154096.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6303..8381)
                     /gene="topA"
                     /locus_tag="IW774_001750"
     CDS             complement(6303..8381)
                     /gene="topA"
                     /locus_tag="IW774_001750"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1154097.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8503..9360)
                     /gene="dprA"
                     /locus_tag="IW774_001751"
     CDS             complement(8503..9360)
                     /gene="dprA"
                     /locus_tag="IW774_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1154098.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9416..10183)
                     /locus_tag="IW774_001752"
     CDS             complement(9416..10183)
                     /locus_tag="IW774_001752"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1154099.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10176..>11029)
                     /gene="ylqF"
                     /locus_tag="IW774_001753"
     CDS             complement(10176..>11029)
                     /gene="ylqF"
                     /locus_tag="IW774_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1154100.1"
                     /translation="QWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPMLDQI
                     IQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKEALK
                     EKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQWLRL
                     GNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYSGRI
                     SDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDRCEE
                     MEQKSDD"
BASE COUNT         2853 a         2459 c         1936 g         3781 t
ORIGIN      
        1 cgatgtcaca ctcccttatt acttacagga tagctttttt acacaccttg gtaaacggtg
       61 ttccctgcta acttctcggt cacaacctct tacttcactg tgatggaata ttttgtcttg
      121 aagatttcat gggcacctag ttgattgatc cctacttttt ccatcaagtt tccactactg
      181 ttcaccgtgt cagcgattcc gcaccacggt tcgatacaaa cgaaaggcgc ttcttttgga
      241 taaggagacc agatccctac ataaggcata tctttatagc ttaaagttac gctatgggaa
      301 gatttttcac ttcgaatggc gtacgcattt agaccccttg tttcaaaaac caaggcatct
      361 tgctcaaaaa gttcacgatt caaagccaga ttcgtatttg tctgacctaa cgtcttttga
      421 tccatatcga tgaatgggcc tttcaatggt aggcgtaatc ttgatttcaa tggagaaaat
      481 gccagataga aatcttcaaa tgacaatcct tcttcaagag gtacattgaa agcaggatgt
      541 ccaccgatag aaaagaacat ttctttttca ccggtatttg ccacttgata atgaaccgtg
      601 atgccgtctc ctcctaattc atagctgatc actaattcaa aatcaaatgg atacttttct
      661 aatgtctccg gcgtactcct taagatgaaa ctagccattt cttgtccatg ttcaatgact
      721 tcaaaatcca tatcccgagc aaatccgtgt tgccccattt gatatgtttt cccttgataa
      781 acatactgat catcttttaa acgtccaaca aaagggaata aaaccggtgc atgtctgccc
      841 caatacgctg gatcaccttg ccagatatac tctaaaccat gttcttttga tttcagactg
      901 atcaattccg caccgtgttc agcgatttcg gcgatcagct ggtcattttg aataataaca
      961 gtcattgcca ttcctccaac ttcgacttac tttttaaaat gggaaggctg gaacaaacat
     1021 ttctatctgc cccagtcccc ttacttctaa taactgtgtt ttgctaactc gtctttaaaa
     1081 cgggaattca atacttttaa ataagttccc ttcatgccta acgaacgaga ttcaatgatt
     1141 ccagctgatt ccaattttcg taaagcattg acgatcacag aacgagtgat gccgatacta
     1201 tcggcaatat tagaagcagt cagacgacct tcatctccat ccaatgcttc aaagatagct
     1261 tgtacagcct tcaattcgct ataagataat gtgttgattg ccatttggac agccgttgca
     1321 ctccgtacat ctgcttcgat gcttcgagac ttctgataaa ggatctgcat accgacaacc
     1381 gtagctccat attctgctaa gactagatcg tcatctgcaa acgattgttc catacgagat
     1441 aagataatcg tacccaaacg ttcgccagct ccaaagatcg gaacgacagt cgtcagacca
     1501 aatggatatt tctctcttga ctctaccgga aatgcagtaa tatcactagt aattgggata
     1561 ttagcttcag ttttcatcaa ctgtgccact gtatcagtat aagattcagg aaattgtttc
     1621 tcaacaaaca ttgttttgat tcgttcattg ttcacatcgt gacgttcatt ataacctagt
     1681 aatactccgt cacttcctat gatataggtg ttgctgtcaa gaataccacc tagtgtgaca
     1741 gccattttat cgtaaggcaa atctgcttgc atgtcaaaag tattcttttg ctgcaaaagt
     1801 gtgtttatct tgcgggtttt cgttagcaag tctgtcattg tcgtcctccg attttttata
     1861 aaatatagcg acttaaatct tcatttgccg caattgagcc aagtttgtca tccacatatg
     1921 cttctgtgat cgttatttct cccatttgca tatctggtgc ttcaaataat agatcttcta
     1981 gtagtttttc caaaatggta tgcagtctgc gtgcgccgat attatctgtg tcacggttga
     2041 catcgaaagc gatttgagcg atccgttcaa ttgcttcttt tgtaaacgta actgacacat
     2101 tttctgtacc gatcaacgcg acgtattgtt ttactaaagc attatttggt tcggtcagaa
     2161 tcttcacaaa atcttcagct gtcaaatcat ctaattctac ccgtatcggg aagcgtcctt
     2221 gaagttctgg aattagatca cttggtttag acaaatggaa agctcctgaa gcaatgaata
     2281 ggacgtgatc tgtttggatg atcccgtatt ttgtgtttac ttgtgaacct tcgacaattg
     2341 gcaatatgtc tctttggacg ccttctctgg atacttctcc agattgctgt gatttagaag
     2401 tgatcttatc gatttcatcg ataaaaataa ttccactttc ttccgctaat ttgattgcct
     2461 cactatggat atcagcatcc ttgactaatt ttgctgattc ttccttgatc aataactcta
     2521 aagcttcttt gactgttacc gtccgttcca cttttttctt cggtgtcaaa gcacctagag
     2581 tttcatttag gtcgatcccc atttgctcca atccattgtt catcattggt gcaggtttct
     2641 tttggtcttc aacttcaatg gttacttcac gactatccag taaacccttt tgtagttgtt
     2701 ccaagactgt acggcgattg attcgaatat cttctgtcac ttcttcttgc ggttcagcgg
     2761 tattttgctg actagcatta aaaagattca tcatttgttc aaattgatta gtggattgtt
     2821 tctgttcttt tttgatacca ggtaccaaga tttttaccaa acgtttttct gcattttttt
     2881 tagcacttgt gtatacacgg ctgtactgct ctttttctac gatttggatc gcattttcta
     2941 ctaaatcccg aaccattgat tctacatctc ttccgacata accgacttca gtaaattttg
     3001 ttgcttcgac tttgacaaat ggtgcattga cgatttttgc caaacgtcgt gcgatttctg
     3061 ttttaccgac ccctgtaggt ccgatcatca gcatattttt tggtgtaata tcttgttgca
     3121 ttttttcatc taactgcaaa cggcgatagc ggttacgcaa agcaacggca acagattttt
     3181 ttgctgcgtg ctgtcctacg atatattcat ctaattcact tacgatttgt cttggtgttt
     3241 gttttaattc atacatatct actcacctct tacagttctt ctacgataat attatgatta
     3301 gtaaagatac agatatctgc ggcaatattt aacgcatttt ctgcaatttc tttcgcactc
     3361 atgtcgtttt gagcatagtt tttcattgct cgggctgcag ccaacgcaaa attccctcct
     3421 gatccgatag ctaagatacc atcatctggt gtgataactt ctcctgtacc tgaaacaaga
     3481 agcatttctt tatcattcat tacgatcagc atcgcttcaa gtttttgcat agattgctgt
     3541 gtccgccatt cttgagctaa ttcgacagct gcgcgttgta gattcccgtt atattcgttt
     3601 agtttgcctt caaatttttc ttctaaagta aacgcatctg caacacttcc ggcgaaacct
     3661 acaacaactt ctccattgta gatacgtcgt actttctttg cagtgccttt catgacaaca
     3721 gattcaccca ttgttacttg gccatctcca gccatagcaa atttcccgtc tttttcgacg
     3781 gcacagatag tggttgaatg aaatggtgat tcgaccatgt ttgtttcctc ctaaatttgt
     3841 tcttttcctt atttttacgc tcttggatgg aaagaacgat agtttttttg taaactttct
     3901 tttgttacat gggcatagat ctgggtagtc gataaattgg catgtcctag caattcttgg
     3961 actgtccgca aatctgcccc attatttaat aaatgagtag caaatgtatg gcgcagcatg
     4021 tgaggatgga tatcactatc caaactgctt tttttgatca tttgattcaa gacatattca
     4081 attccagtag gcgtgattgg ttttccatga tgattcacaa aaacgatatc atgctcttcg
     4141 tgatattttg tcatcaatac ctggcggcct tccctgaaat attcttgcag agcatcttga
     4201 gcaaaagaac ctagaggagc atatctctct ttgttccctt taccatggat caacatgacc
     4261 tctgaatcaa aatcaatgtc tgataccgac aaactgctac actcacttaa acggatgcct
     4321 gacccgtaaa ggacttccag taaagcgcgg ttcctcaaat ccaaaggact atctcctgct
     4381 acgctgtcaa aaagtgcttg catttcattt tcataaaaaa aacgtggcag tctcaaattt
     4441 ttctttttga gatgaacgta agaaaatgga ttttctttta tcacttcttc tttgagaaga
     4501 tactgataaa acgaacgtag gctggctatc ttgcgactga tagaatttct gctatagcta
     4561 ggacgatgat cagctaatgc actcaaatag acacgaaccg taaaatattc cgccttcagc
     4621 agatcagcgt ctccagattc ctccaaaaaa tgtttgaaat ggagaatgtc ttcttcatat
     4681 gcttctttcg ttttttctga atatcctctt tcgacaatca aataacgcaa aaatttttgt
     4741 tcccacgtgg cttctgtcat tttctcacct tccttttgtc ccagagaata atttagcaca
     4801 gcgtttataa aaagacaaat aaattgtcag aatttaacca atatttctaa gtttgttcac
     4861 aattggcgca attcctcaca ttttgtttga gaaattctta agaaaaacgc aagaaaaaga
     4921 gcgagactat ccgtctcacc ctcttcatgg agtttcctta ctcatttggg attgcagttt
     4981 tttctaattc tgtttttatt gcctcattag cttttaaagc tcgttgtgca attgcttcat
     5041 atcttgcttt tttgtctcga atgcgctctg gcagttcagg aaacagacca tagttggcat
     5101 tcatcggttg gaaatgtttt ccttccgcat gagtaatgta gtaagccata cttccaatag
     5161 ctgtttcttg tggaaactcg actggttcca tttcttttgc taagcgtgcg gcgttgattc
     5221 cagcaagtag tccgctcgct gcactttcaa catagccttc tacaccagtc atctgacctg
     5281 caaagaacag atcttcgcgt ttcgttgatt ggtaagtcgg tttcagcaat tctggcgaat
     5341 tcataaagct gtttcggtgc atcacaccat agcgaacgaa ttcagcattt tctaacccag
     5401 gaatcatccg gaaaacacgt ttttgctcgc cccatttcaa gtgagtctga aagcccacga
     5461 tgttgtataa agaagctgca gcattgtctt gacgcagctg gataacagca taaggacgtt
     5521 tgccagtttt aggatcttcc aatccaactg gtttcatagg tccgaaaagc atcgttttga
     5581 atcctcgctg tgccattact tcaattggca tacatccttc aaaatatttt tctttctcaa
     5641 aagatttaag ttctgccact tcggcattga ttagtgcctc atggaaagct ttgaattcct
     5701 cttctgtcat cggacagttc aagtaagctg cttctccttt gtcatagcgg gacttcaaat
     5761 atactttatc catatcgatc gtagctttgt ctacgattgg tgcagctgca tcatagaaat
     5821 aaaaaccatc tgaaccattg aatgctttga tgctttctgc taatggatga gaagtcaaag
     5881 gtcctgttgc tactacggtg attccttccg gtatttcagt aatttcttcg tttatcactg
     5941 ttactaatgg atgctctttg acttttcggg taattgtttc agaaaaagaa tcgcggtcaa
     6001 cagctaaggc accaccagct ggcactgctg tttcatctgc cgaagtgatt actaccgaat
     6061 tcaatcggcg catttcttct ttcaataacc ctactgcatt agccaaactg tttcctctta
     6121 atgaattgga acatactagt tcagcaaaat tacttgtgtg atgcgcctca gtcgatttga
     6181 ctggacgcat ttcatataaa cggacaggga ctcctgcttg tgcgatctgc caagcagctt
     6241 cacttccggc aagccctgct ccaacaacat ttaccatttt caatgaaaga acctcttttc
     6301 tattattttt ggatgtcttc ttcgtaatct ccatttacac agactacttg tttaccgcct
     6361 ttgactttct tctcgaccag atatccgtca cattttggac aatttctgcc cactggttta
     6421 tcccaagaag tgaattcaca ttctgggaaa cgggaacaac catagaatag tctgtttttc
     6481 ttagattttc gttcaatgac ttgtccttca tgacatactg ggcaagtcac accgatttct
     6541 tttacgattg gtttcgtatt ccgacaatca ggaaaattac tgcaagcata aaacttccca
     6601 tatttcccta attttatcac catcggatgt ccacagacat cacaatcgaa tcctgctggt
     6661 tcatctttga tttgaatttt ttcgattttt tcttccgcat tcgtcagttc tttttcaaac
     6721 ggtttataga aacgatcaac cactttgacc cacgcttccg tgccttcttc aattttatcc
     6781 aaatctcctt ccatttctgc tgtgaaatgg atatcgacga tttgagggaa gaattcacag
     6841 attaatgaat tgacaatttc tcctagttca gtcggctcaa atcttttttg tgtcaatttt
     6901 acatagtagc gtctttggat cgtatcaagt gttggtgcat aagtggaagg tcgccctacc
     6961 ccgttttctt ctagtgcacg aatcaacgta gcctcactaa aacgtgctgg tggttgagta
     7021 aaatgttgtt ttggttcaat atcaacagat tggactacat cgccttcttc aagttctgga
     7081 agaatatttt ctttctcttc tttgccgtca tctctacctt ccacatacac ttgcatgaat
     7141 cctttgaatt tgatttttga accgttcgca atgaagatga caccattttg ttctaatgtc
     7201 actttcattg tatctaaaat agctggtgtc atctgactag ccacaaaacg tgaccagatc
     7261 aatgtataaa gtttcaattg atctttatct aaatattgtt tgagttcatc tggtgtacga
     7321 agcacactcg ttggacgcac agcttcatgg gcatcttgtg cgccttgagc atttttgatc
     7381 tttcggtgat catgtctaga aaattctttt ccatacgtat cttcaataaa tgctgcggcc
     7441 tctgcttttg ctgtatcggc aatccgggta gaatcagtac gcatgtatgt aatcaagccg
     7501 actgttcctt gtttacccaa agtgattcct tcatataatt gctgtgctac catcatggtt
     7561 tttcttgttc gaaagtttaa cttacgggca gcttcttgtt gcaggctact tgtagtgaaa
     7621 ggattcgcag ggtttctttt gcgctctttc ttttctactt ttttaacttc gaagtctgtt
     7681 ccatcaattc tagcagtgac ttcttttaca ctttgtgcat ctggtaattt ctttttctta
     7741 ccgtcgattc cccagaaatt tgctttgaat ttcttgcgag cttttttaaa attaccgtcg
     7801 atgctccaat attcttctgg aacaaattct cggatctctt tttcacggtc aataataatt
     7861 tttaaagcga cagattggac acgtcctgca cttaaccctt tcttgacctt acgccagaga
     7921 ataggactga tcgaataacc aacgattctg tctaaggtac gacgagcttg ctgtgcatct
     7981 actaaatcta catcgatcgt tcttggttcc ttaaaagctg ctttgactgc gtcttttgtg
     8041 atttcattga agaccacccg atttttttct ttcagatcca acccaagtag gtacgctaaa
     8101 tgccaagcaa tcgcttctcc ttctctatcc gggtcacttg ccaagtaaac tttttgagct
     8161 ttttttgctg cggctttcag gcttttgatg acatcgcctt ttccgcgtat agaaatataa
     8221 tggggttcat aattgttttc aaaatcgata cccattttac ttttcggtaa atctcttata
     8281 tggccaacac tggccattac tttatagttt cgtccaagat atttctcaat tgtcttggct
     8341 tttgcaggtg attctacaat cactaaatat ttatacgcca ttactgaatg gactcctttc
     8401 cattgtcgtg ttcctactat taaaagagga ttcatacaaa tcgtagctaa tcatatccat
     8461 tcagaaaacg tttgtcaaga aaagcaaatg aaaagtatgg tgttattctt taaattctgc
     8521 caagatatct tcacccgaaa ccgtgcaaac tgcaccgtct tggatcaaat gatgacatcc
     8581 ggtagaatgt tctgttaaaa tatttcctgg aacagaaaaa actgttcttc caagatccaa
     8641 tgcttgctgt gctgttatca gagagccgct ttttttcttg gcttcaaaga cacatgttcc
     8701 tttagacaaa gctgcgataa tccgatttct cattggaaaa caatgctttt tcgggccgga
     8761 ttgaggagga tattcactca aaagaagatg atcatttgca atcacctctt gcaagtcacg
     8821 attttcagca ggatagactt gctctatccc tgttccaatg actgcgattg tccgtccacc
     8881 atgagagatt gctgcttggt gaccgtatgt atctatccct ttcgcgcagc cactgacgat
     8941 cacatatcca gcttctatca ctggttttag taaataataa actgtttttt tggctaaagc
     9001 agaagtgtgt cttccaccaa caacgcttaa catgttacaa gaaagtaaag atagatcacc
     9061 attataaaac agtacaattg gcggataggt caagtgaagc agctgagggg gatatttggg
     9121 atcaaagcaa gtgataaacg actgtttcga ttggattttg ccccagttag cgtgaatttc
     9181 ctgccaactt ttgcgaaaca gctcccggtg tttggttatt ttacctatct taatgatgtc
     9241 ttcaaatccg atttctttat aaggatactg catcatccag ttagcaagtg cccattttcc
     9301 ttgactactg attccttttg tataagcaag tttcaataaa ttttcttcta tttgatacat
     9361 cataaaaccg cctttcgtct ctctttctaa taagatacgt aaaagcggct gttttttatt
     9421 gtaaatattt ttttacaggt gcaaaacttt tacgatggat tggcgtaatt ccttgtcgtt
     9481 caagtccttc taagtgttcc tttgttccat aacctgcatt atgcgagaaa ccatatccag
     9541 gataaagcac gtcatattct ttcatcaatt ggtctcgata tactttagca atgatacttg
     9601 cagcagcaat cgacacagat cgtgcatccc ctttgattat tttttcctgc tgaataggca
     9661 aatccaaggt catcgcatcg atcaacaaat agcttggtac aatggacaat ttttctactg
     9721 cttggatcat cgctaattta cttgcttcat aaatattcac atcatcgatt gtctgctgat
     9781 caacgatccc gatcccgatt gccagggctt tttcttgaac ttgttgataa agaagttctc
     9841 gctttttctc tgataattgt ttggagtcat tcagccctaa tatgatctca tttttcggca
     9901 agataactgc agctgccacg acaggtccag ctaatggtcc tcttcctact tcatcaatgc
     9961 cggcaatcca ttggtgacct tgtgcgtacc cttgcttttc aaagcaaaac atttcttcat
    10021 atttttcctt aattaatgct tcctttttca atcgtcgttc aaaagaagcc agtgcttgct
    10081 gcacgccttt gcgtggatca tttcttaatg attctagtgt aggatcatct attctctgta
    10141 tgcttttcaa tcgttctttg atactttgga tggattcagt catcactttt ttgctccatt
    10201 tcttcgcaac gatccagtgt gtatcgtcca agttttcctt gacgaatatc atgcacgact
    10261 aattcgctgc cacgatcata gtcttcttta tacccacgtt ttgctgtaat atccatcagt
    10321 aattccggtg ccaatttttc ttcttgctcg attgataaac cataacgatc gctgattctt
    10381 cctgaataat atcgggcaaa aaagtctaat ccgtaaatcg ctaaatcatc taaatggaca
    10441 agctggtctt tgatcgcgcc tgtcagcgct agttttttgc cgatttcttc atcttcaaat
    10501 ttgggccata aaatccctgg tgtgtctaac aattccaact cattacctaa acgaagccac
    10561 tgctgacctt ttgttacgcc aggcttattt cctgtctgtg caattttctt gcctactagt
    10621 cgattcatca acgtagattt cccaacattt ggaataccta agaccattgc ccgaatagcg
    10681 cgaggcttga tcccccgagc agcatctctt gcccgttttt ctttcaatgc ttctttagcc
    10741 gcaggtacta gacctttaat tcctttactt tcttgtgccg tgatagctaa tgcaagatgg
    10801 ccttgtttac gaaaatattc ttgccacagc tttgtctgtt gtggatctgc cagatccgct
    10861 ttattcaata aaacaaggcg aggtttttgc tgaatgatct gatctagcat tggattacga
    10921 gaagataaag gtaatcgtgc gtctaccaat tcaaatacga tgtccacata tttcattttt
    10981 tcagaaactt ctcgtcgggc ttttgccata tgtcccggaa accattgga
//
LOCUS       DACEOE010000083        10780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEOE010000083 DACEOE010000000
VERSION     DACEOE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.085"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(99..2225)
                     /gene="pnp"
                     /locus_tag="IW774_001754"
     CDS             complement(99..2225)
                     /gene="pnp"
                     /locus_tag="IW774_001754"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1154101.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2413..2682)
                     /gene="rpsO"
                     /locus_tag="IW774_001755"
     CDS             complement(2413..2682)
                     /gene="rpsO"
                     /locus_tag="IW774_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1154102.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            2933..3496
                     /locus_tag="IW774_001756"
     CDS             2933..3496
                     /locus_tag="IW774_001756"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1154103.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3749..4270)
                     /locus_tag="IW774_001757"
     CDS             complement(3749..4270)
                     /locus_tag="IW774_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1154104.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4273..5115)
                     /locus_tag="IW774_001758"
     CDS             complement(4273..5115)
                     /locus_tag="IW774_001758"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1154105.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5108..5833)
                     /locus_tag="IW774_001759"
     CDS             complement(5108..5833)
                     /locus_tag="IW774_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1154106.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6009..6305
                     /locus_tag="IW774_001760"
     CDS             6009..6305
                     /locus_tag="IW774_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154107.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            6873..8465
                     /locus_tag="IW774_001761"
     CDS             6873..8465
                     /locus_tag="IW774_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1154108.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8478..9218
                     /locus_tag="IW774_001762"
     CDS             8478..9218
                     /locus_tag="IW774_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154109.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            9386..10735
                     /locus_tag="IW774_001763"
     CDS             9386..10735
                     /locus_tag="IW774_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1154110.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
BASE COUNT         2979 a         2162 c         2153 g         3486 t
ORIGIN      
        1 gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt ccccctgtca
       61 ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc atttttcgtt
      121 gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg tttatcgatt
      181 tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac tttattgaca
      241 cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat caagttgacg
      301 aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc aactttgact
      361 tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc atcagctgaa
      421 gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc atcgatgatt
      481 ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg ttcgatctgg
      541 atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc aatcgttgaa
      601 gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc ttcagttagg
      661 atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat accatctttt
      721 gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg gatatcagtt
      781 aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc gactggcgct
      841 ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact tgcttgggaa
      901 gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg aaattcttct
      961 tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc gatttcccga
     1021 cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa attgtagtgg
     1081 tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg ctctcctaga
     1141 ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa acctgatcca
     1201 tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc caatttacgc
     1261 ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt ttcaagatct
     1321 tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc tgcaaatttc
     1381 tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc gcgagcaagt
     1441 ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa gatttctttt
     1501 ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc tactgcttga
     1561 acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa cagcaatgca
     1621 ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt gatggcttgt
     1681 ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac tgttggattc
     1741 aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc gtcaaatggg
     1801 atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc tggcgagcag
     1861 tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa gccttctgca
     1921 aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc cgtacttgga
     1981 cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat tttttcttca
     2041 taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc cacagctgca
     2101 cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg ttttgctagc
     2161 tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac ttgtttttct
     2221 gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa atgaaaactg
     2281 ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct agcttgatca
     2341 cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga atcccactct
     2401 ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt aacgttgaac
     2461 gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt tcattaggcc
     2521 gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt ggttgatttc
     2581 ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat ggcgagcgta
     2641 ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca cctctttcga
     2701 ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg caggtcatgt
     2761 gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt agttaatcat
     2821 taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt ggtaaactat
     2881 aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt ccatgattac
     2941 aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa aagaagtttc
     3001 tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt tcttaaaaaa
     3061 cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg gcttagctgc
     3121 tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc ctgacccaga
     3181 ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga gccattctgt
     3241 tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag aagtaccagg
     3301 atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg gtgaaaaaca
     3361 caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg accatatcaa
     3421 cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa aagaaggcgt
     3481 attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa gaaagcttta
     3541 taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc actaatcgaa
     3601 atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg atacgcatct
     3661 tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga tagaatagtt
     3721 tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct gcttcagcta
     3781 gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta tgcagatgga
     3841 tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt ctcataaagg
     3901 aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg taaaggggat
     3961 gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc agttcttctt
     4021 ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta ttggtttctt
     4081 ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca gctcgtagta
     4141 aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca aacttgctag
     4201 cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc cgtgtctctc
     4261 cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt gtttcatcaa
     4321 atcaacatac gtgtctgtta ttgtttcctt atatccctta tttttcagca gactaacaac
     4381 tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt cttctttttt
     4441 cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa ccaactctcg
     4501 atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta gttcttcctc
     4561 tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg gaaaagattt
     4621 ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac ccggcgtgac
     4681 gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg cgtactccag
     4741 accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct ggacattcgg
     4801 agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg tacaattgaa
     4861 atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat tgataactgg
     4921 ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt gggatcgcag
     4981 ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca tacggtcggc
     5041 aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt ctttttcggt
     5101 tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca accgctccgt
     5161 cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg attactaaga
     5221 ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact tgaaaactcg
     5281 ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc tccatgactc
     5341 catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct ccgcatgctt
     5401 gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat tttcgacaat
     5461 gacattgata cagactccca tgaaaattga cgagttgcgg gcttttagcc tttgcgtgca
     5521 aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga tgtgctattt
     5581 ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca aattgataga
     5641 atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc tgctccatcc
     5701 cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg gatacccctg
     5761 caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct tcgtctactg
     5821 ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg aacaaattta
     5881 ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga gcaatttatc
     5941 acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata aaagtgaggt
     6001 gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt atcgcctggt
     6061 tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat ggaaatcttt
     6121 gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca ttagccgtat
     6181 atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta atattagcca
     6241 tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag aaagcggcag
     6301 cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc tttttttatt
     6361 gtattacata catttttgtg tataattata ctatcgtttg caatggatag atgattcaca
     6421 ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt ttttcaaaaa
     6481 ccagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg tataaataac
     6541 tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa ccctgtggag
     6601 tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac ttttttgctt
     6661 ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt gatgctaagt
     6721 tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct ttttatatct
     6781 ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat ctagaccgct
     6841 cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt tcagttgccg
     6901 tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat gcagatgaaa
     6961 aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc ttcaactggt
     7021 ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt tacgccggcg
     7081 gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa ttagtcatcg
     7141 tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc gacgcgatca
     7201 tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc gatccttatt
     7261 acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa gcaaaaagtt
     7321 tagcagatct ttctggagca aagatcactg ctcagttaaa tacattccac tatactgtga
     7381 tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca gcaatgagaa
     7441 cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa ggtgtaacgg
     7501 caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat ggcttccaga
     7561 caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg gatatccaaa
     7621 aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt atggaccaag
     7681 cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc atttctgatt
     7741 tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga cttaccttgt
     7801 tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt ggtgtcttcc
     7861 gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc ggaaatgcac
     7921 ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa gcaatggtca
     7981 tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt gccgctttgt
     8041 ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg gggatttttg
     8101 cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat ggccaaacga
     8161 tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca ggaaatgagt
     8221 ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct gatttgttct
     8281 tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt acgatcgtcg
     8341 ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg gtagaaaaga
     8401 aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca aatttgcagg
     8461 aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta agaaaaagct
     8521 tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt gaagttgtga
     8581 cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc aatttactgg
     8641 aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct cctaaatata
     8701 atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac ctgttcaaca
     8761 atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa cgagataaag
     8821 aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat cgttatatcg
     8881 aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt gctcgggcat
     8941 tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta gaccctgaaa
     9001 tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg acaatgctta
     9061 tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt tttatggata
     9121 aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg aaagaagaac
     9181 gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg attgggacaa
     9241 agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg taaatttctg
     9301 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
     9361 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
     9421 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
     9481 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
     9541 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
     9601 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
     9661 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
     9721 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
     9781 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
     9841 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
     9901 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
     9961 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
    10021 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
    10081 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
    10141 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
    10201 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
    10261 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
    10321 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
    10381 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
    10441 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
    10501 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
    10561 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
    10621 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
    10681 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
    10741 tagttgtgac agaagtggtc agcttcgaga aataagaagg
//
LOCUS       DACEOE010000084        10697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEOE010000084 DACEOE010000000
VERSION     DACEOE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.086"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            299..1852
                     /locus_tag="IW774_001764"
     CDS             299..1852
                     /locus_tag="IW774_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1154111.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(2233..4017)
                     /locus_tag="IW774_001765"
     CDS             complement(2233..4017)
                     /locus_tag="IW774_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154112.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(4032..5753)
                     /locus_tag="IW774_001766"
     CDS             complement(4032..5753)
                     /locus_tag="IW774_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154113.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            5853..6614
                     /locus_tag="IW774_001767"
     CDS             5853..6614
                     /locus_tag="IW774_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1154114.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            7581..7849
                     /locus_tag="IW774_001768"
                     /pseudo
     CDS             7581..7849
                     /locus_tag="IW774_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            8521..>10697
                     /locus_tag="IW774_001769"
     CDS             8521..>10697
                     /locus_tag="IW774_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154115.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         3478 a         1804 c         2143 g         3272 t
ORIGIN      
        1 gccagaatga acttcattct gtctcagatt ttttgttatt tcaaattgat taattgattg
       61 atcaaaaagg ttttgatttt catgttctca aacacgttca atgtcttttt cttctgaatg
      121 gtcttgtaag agatctctgt aatcatcaaa tttttgtcta ttaatcttag tattttttga
      181 tgtataaact acctgaaatt gagagtacat accaaattca taaatttttt attagtggga
      241 ctagtgattg tatggatttt gacgcataat gaatgaagta tgagaaaagg agagacaaat
      301 ggatcaagaa ctatttatta tcgaagtatt atcagctaaa caaaaaatta tcgagaaagc
      361 gaaaaaaaga gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt tctctggttt
      421 gctttttttc ggagctgtat cgttcctata tcgccatatg agtgcagtag tccgttcagc
      481 tgaagggatg caaaaattaa ataatttgta tcacttttcg tggggagttg gcggaataac
      541 aattgttgtg cttattgttt ttcttctact tatttggctc cgttaccatc aattgtcgcg
      601 tcatttcgat caatggctta gtcaaagagc acaacagttt ttacataata aaccaacttc
      661 tcaagatgat gactatcttt attttgagaa gacttcgacg agtcaagctt ttcggataca
      721 aaaaagtgat tgccagttat tgctgagatc acttgatgat ttacctatct acttaggcga
      781 aagcaaatca aaattcggaa tgaacacatt ccatattttt ttactagaaa acgaacccag
      841 atgggaaaaa agccagtctt cgcctggtgg atacctgaca actaagatga gtacattagt
      901 cactggtttt ttcctcattc ttttagctgg aacaatctat tatgggagat acactcagcc
      961 aaaaatgtct tctcatataa atgctgcttc tcacatgaca atgaactcaa ctaacgatac
     1021 gcatatacaa aagacaaatg aagggttgtt agctgatcct aagcaaaagg taaatttgac
     1081 aacacaaaag ataaatgatc tcaaacttga tacagagacg cactctctct atatgacaac
     1141 agatagtggt gctaattggg tatttgtgcc gattgattcg caatggttgc gttttggcga
     1201 atacaccctg acaacggggc tgattcctga aggttattgg atggacaaca catatgatat
     1261 ttccgccgat ttctcatggt ttatctattc ttctggggat gatgtacgtc tgctgacttc
     1321 aagagataat ggtaaaacat ggcaaacgtc tattatcacc caatcagttc agccgatacg
     1381 ttatcgaaaa gcggtatttt atggcaacga tggtctattt atctgtacat cagccagtgg
     1441 gatgtctgca gaaactttgt acatttacac tactatggat ggcggaatca cttggataaa
     1501 aagtggaagt acaatgatca atcagccagt acaaaatgtc agcttcgtta caacttcttt
     1561 aggttttgtt tcgacacgtg aaagtttgtt ttatacaaac aacggaggac gctcattcaa
     1621 agaatcagtt ctgtcgattc cggaaaacta tcagacaggg gggatagatc tttttcaatc
     1681 accaaatgaa gttactcaaa taagcgcgaa tacgttagag acaaaatttt atttgttgaa
     1741 aaaaggacca atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa ctgataacgg
     1801 ggaaacatgg caattcgttc agcaactatc gcaagtagaa caaggagatt aaagaagaga
     1861 gattgtgagc ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa acagcttctc
     1921 atatttgttg aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa gaagcgattt
     1981 tgaagtatag cttctcgtat tttacaagat cgtgtcttat tgccgatagg ttgtctcttg
     2041 aacaccgttt attcgtaaaa agggattgtg acaagtcttt tgtcactact taccgaacga
     2101 aactgaagga gctatccaaa aagtatcatt agtattattc tttgtgtttc tagtggcacc
     2161 actagtacat ttaaaaaagc tatcgatcat ggactaatca tacatgatcg atagcttttt
     2221 agataatgtt tgttacgcta tctgaaattg actattataa agactggcat aaagcgttgg
     2281 tgattggagt agttcagaat gtgttccttt ttcgacgata tcgccattct tcattacaag
     2341 gatcatgtct gcattttcaa tcgttgacaa acggtgtgca atcacaaaac ttgtcctatc
     2401 ttttgtcaca gcttccattg cttgctgaat caatgcttct gttcgtgtat ctacgctaga
     2461 agttgcttca tctaaaataa cgacaggagg attcgctaga atgactcgag caattgtcaa
     2521 taattgttgc tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg tatcatatcc
     2581 gtctggcagt gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt cgataatttg
     2641 ttctcttgtg gcatccattt tcccgtaggc gatattatca gctaccgttc cttcaaacag
     2701 ccaggtattt tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg aaagcttggt
     2761 aatatcaaga ccgtcgaatt cgatctttcc tttattcagt tcataaaaac gcatcagcag
     2821 attgactaaa gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg tttgttttgg
     2881 ctttactgaa aaatcgactt gattcatcaa tattttatct gatgtgtaac caaactgtac
     2941 atttttgaaa gctatttttc ctttcggttg ttctaaaagt cttgcatctg gaaggtctgg
     3001 aatttcatct gtttcatcta atatctcaaa tatcctatcg attgctgcca ttgcggattg
     3061 gatcgagttg atcacataag aagcggtaga aattggttca gaaatctgat tgatgtactg
     3121 aagataagct tgtaagaagc cgatcgtcac accacctgat agaacaagca tagcaccgaa
     3181 taccgcactt atgatgaagg cgagttgatt aatgaaacga atggccggat agatagcaca
     3241 gttcataaat tgagcacgtt taaaagcgtt gtattgcttt ttatttactt cgtgtatagc
     3301 agcttctgca tgagcttgct gattgaatgt tttgaccact agattaccag ctaagatttc
     3361 ttccattcga ttatttaaaa cacccagttc atactgattc tgttcagaaa tctgtttgtt
     3421 tttgttcgca atcttagttg taacaattga actgccaacg atcagcaata agacgagcaa
     3481 agtaagcttg ctttcgatat aaaacagcat cagaccagca gcagctattg ttacgacaga
     3541 agtaaaaaat tgattgatac ctgtcaataa cacttgagaa agttgattga gaccggtagt
     3601 tgtccgacta atgatgtcac ctacttgatg attgtcaaaa aacgacatcg gtaatgtttt
     3661 gaattttttt gtgatttctt ttcgcaccct caatgtgatc ttttcgctta acgctgccat
     3721 tgtccgttcc tgaataaatg aagtgatgct gcttaagact gaacagccga tcagcaataa
     3781 gacagggaac aacaatgctt gtttgatatc actgaaagaa agctgtccaa tacctgtttt
     3841 tcttatttct gataagagat catcgatccc gattcccatg atcaagggca tggctacgac
     3901 taataggttc ccgaataaac tgcatagaaa caatccgtaa aaatatttat gttcaggttt
     3961 gatcattttc caaaaacgta tcaaagcacg cgtgttcaac gtttttttct tttccatcgt
     4021 atcccttctt tctatattcc ttgtgatttt gcaaactcct gataagtttg gcaattagac
     4081 agtagttctt catgagtacc ttgtccaacg atttttcctt catctaaaac aatgatgtta
     4141 tctgcttgtt ggattgtact gaggcgttga gcaacaatca cgagagtttt ctccgacatg
     4201 tgtttatgaa gggctgctct taatgctgca tctgttttat agtctaaagc ggagaaacta
     4261 tcatcgaata catagatctc tgctggtttg atcaacgctc gagcaataca tatccgctgt
     4321 ttctggcccc ctgaataatt agaaccgcct tgagcaacaa agctgtccaa tccgtcagga
     4381 agggtgtcca ctagttcttc taattgagcg atcttgatcg ctttagacaa ctcttctgtt
     4441 gaggcatatt catttcccat ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc
     4501 ttctgaggga cataactgat gtgggagcgg atctgttctt gagtcatttg agtaagagga
     4561 ataccaccaa gggttacttt tcctatcgta gggtcactaa gtctcaagag aagtttcgcc
     4621 aaactacttt ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt
     4681 gtgaaatcaa tatcttctag aactggatca gagatttcag tatatttaaa tgtgacatgt
     4741 tcaaaagcag ctgcatttgt ctgatcaaag gttggttcag ctactgtttt gatcgtctct
     4801 tcctcaattt cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat
     4861 ttagggataa tgaccaatac catagctgcg atcatcaagt aagcaagtgt cagcatagag
     4921 tattcgacta cagcagtcac gacaccgatt tgtaatgctc cttctgctac aagatttccg
     4981 ccaaaccaca aaatagaagc ataaacgatt cccatgagca aaaaagcaat tggagtgacc
     5041 caagcaaata ttcggttcac tttgatcatt tgattagcat agtcagcaaa gctcttattc
     5101 gttcgctttt cttcatatga ttgattatca aaggcacgga tcatattaat cccagtaaag
     5161 aattctctca atgtgatggt gatccgatcc atttttggct ggatactcaa agataaaggt
     5221 gcccctcgtt tcatcagtcg ataaacaatc catacataaa aaacaacaga gatcaatgta
     5281 acccatgtta gtaatagaga ataacgaaac atcatgagaa tggtgaaaac gcaaataacc
     5341 ggtgctggta agatcatttg gcaaaataaa acaatcattt gctggacatt atcgacatca
     5401 ttcgtcattc ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt cattgacagc
     5461 tgttggaatt tacggaaata tttctcccgc gtttggaatc caaacctagc ggaagccaat
     5521 gctgaaaagt aactagaggc aactgcagcg attgttccta gaaacgctac aagcagcatt
     5581 tcaactccaa ttttttttat aactgaggct tcgccacttg ctatacctgt gtcgatcagc
     5641 tttgctacta attttggtac acctaatgta ccggcgattt ctaaacagat catcacaaag
     5701 gtaaggagag cgagtccttt gtttttctgt aagaatgttt ttaagataga catattattc
     5761 ctcctttttt cctgatactt ttcgattcta taccttcaag taacttgaag gtcaatagaa
     5821 aaaagaataa actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg attggccaag
     5881 tatcggaact atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa
     5941 tccgttcaag tagacaagaa gagaatgatt atcgattgtt tgatatcgat gatattttta
     6001 tgctttatga tatcgttttt tatagaaaat tagatatacc gatcaaacag atgaagaatt
     6061 tatatggaaa aacattgacg gagttatatg agacacttga tgaaacagaa agaagaatcc
     6121 ataaagaact ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt
     6181 tgaaattgat gatcgataca aacgaggaag aatttcctgt agaagagatc ccttttgact
     6241 gtatgatctc aacggagttt gaagatattg tggaaatcaa aaaatttcta cctaactatt
     6301 caagctttgg gatgatgagt atgccagctt ccagttcaca gacgatgtat ggatttttta
     6361 tcgaccctag tgaagtacat ttatttactc aagacgtgat ttgggagaaa aaagatacgg
     6421 ctgtttatcg tcgattctta ttgaaatccg agatgaacca tgcggaacgt aataatattt
     6481 tagaaatcag agaaagaatg tatgaaaaag gatggaaaac aggagaagtg atcggccagt
     6541 atctattgac gaatacggat gaaaacaata tcagaactga atattatcat gcatggatag
     6601 aaatgaaaaa ataggaaata tgtgtttaag ataactcgaa aatgatggtt gttttagtta
     6661 taaagaaaaa ttttctggtt cgattacggg tttaatgtgg gggattatta gtcgtggata
     6721 gatagaattt tagtttttta gaggaaatat gtctttaaaa atggaatggt tgcaattctt
     6781 agcgatatat acgatgtaat tgtcctctta aaaaagtatg gaaaaataat tgtattgggt
     6841 gttatgtata attcatggtg aactgagact aatgagaaga aaaattcata ttgttatgag
     6901 tgagattgta tcagatgaga atgataaatt aaataggtgc ataaaagcat tacacttaaa
     6961 atacattgtt gcttacatta ttacaatatg ttaataaggc aaaaataaat agatggattg
     7021 aataaacata ttttttaatc aaatagtccc tactgtgact ttcctcagct atcggtgata
     7081 ctaccgatag catttttttg cttataaaat aattataagt atttagagat ttttcttcta
     7141 acattcttca aacaagaaaa atcacaaagg ttattgttat ttttatatat tctttttaag
     7201 aatgattgtt tttttataga aattttaaaa aataaaacac gttattgatg gatagttagt
     7261 ttgttatttg gtaatattat agaagttgac tggtagtgat ttatttttcg tcgttttata
     7321 aaaatgttat tttttggaaa aattcacaaa tatgtaacga atgtttttct ggaatttgct
     7381 gtgttaaaaa gcagtatgac taatataaat actattgtca tttatgcaat tttttatgga
     7441 tagtaatttt agaggggaat caatgaagga agaatagaag ggaaaaatag ttgcaaatac
     7501 gccagttagc aaaaatagca atacatcttc aagctttaaa attcgagaga atcatcttgg
     7561 tagtggaatc aactacaaca aaaggattaa accaattaat agatagaatt accaatggaa
     7621 aaatagaaaa ggtagttatt ctatacaaaa gttgtttaat tcgcttcggt tatgaactta
     7681 gtgaaaccct ttgcaatata tggtacagaa tatagaaatt attgacatta cagaaaatga
     7741 tgaagattta atacagattg tcactatttg tagatacctt ttacaaggaa accgagccaa
     7801 taaagctaag aagatgatta aggagttgat ggaagacgat acaagttaag aaagtgaaac
     7861 tacgcccaaa taaagagcaa aaagagtaat tggacgtgga aaatgcaaga aatgaaccat
     7921 cgattcggtg gacagtttat tagtaatgac ggtttgagaa catccatgac gagaatgaag
     7981 gaacatccta aatgcgttta gttgaaccaa gtgccaaata atatggccaa ataagcgatc
     8041 agttatcgat gaatcccaag tattctaaac tatatattca ttttgatgct aagcattgat
     8101 atattttttt gggattgatg aaggttaacc aatacaggga ctcactgatg aaattctaga
     8161 gtctgacctt ggtatcaacg atttagtgat tgtttctaat ggaaaagtat aaaagtatat
     8221 aagtacatta acaaaataac tattagggtt acgctccagc ttgtgcaagg cactggcagg
     8281 aggaatcgtg aaatcataat tatggttaag ccacacggac aactaaaagt catacaaagc
     8341 tatcgaatca atcaacagaa gacaatcggc aatcattagc tttgtttaca gcttacattg
     8401 caacgcttga gttgaaccga aaatacgagg gaggtaacgc agccattttg agttggggaa
     8461 tctttttaga aaattatcgg gtaccttgtt gtttagtcaa aattataagg gggagtaata
     8521 gtgaggaaca aatttcataa actgttaagt atgtttctag ttatgcttat tgtgatcaat
     8581 caatttggga acataacggt tctagctgag tctttagcag aacagccaga gcgttcgctg
     8641 acgatcgaag ttgatcaaga ggaggctgct ttgggtgagc agttccagtt gcgagtcaga
     8701 ggaacaccag acctgctaaa tgcattggtc attgataaga gtgaggattt gattgaaggg
     8761 gaagtgaagc aactcaatga ttacgaacgt ctgattactt attcttccca gcattcaggt
     8821 aaatacgaat tgaccgcaag aagcggtgat attcaatcaa atacggtctc aatagaaatc
     8881 agtgtaccag aagaaagcac aagtgagtct tttaatgtac cagaagaaag cacaagtgaa
     8941 tcttttaata taccagaaga aagcttaagt gaatctttta atgaagccag atcatctgag
     9001 ccatctataa cgcaggaatc tcagattgaa agacaagagg agctcttgaa ttatgacatc
     9061 ggcatctctg ctgagcgccc cactttgccg agcaggcata cacaaacgtt aaagggatac
     9121 attcaaggag gcgctggcga tattcttgaa gaatatgggg agatggtcgt acgagttcct
     9181 agagcatcga ttggcttaat tgagttcatt agagtggagg tcgctgatga ttcaccttta
     9241 aagccactgg cattttacca agaaggcaca gacgttgttt ttccatttac ctttgattat
     9301 gaaggattaa atgaagccgc tcagtcgtat acattcagta tcacgtttga agttgctttc
     9361 tttcaatcag gcggtactga gccaaataat gactttcaag tgagtcttac ttatgctgag
     9421 cgagcagcaa acacggaatt ttcaattacg cctattcctt catcaagacc cattttttcg
     9481 aaattatact atggctctag agaaatcgac agccaaggaa atcttgggaa tctgctgatt
     9541 gacgggcaag acgtgaccaa cagtaataaa tttattttgc aggtaaatta tgaaggatca
     9601 cgaaaagacg gtgtagtggt taccgatgta ctaccgcaag gaacaacttt ggcaccgaat
     9661 ccgactacat ggagttccac tggaaataga gaacctattt caggtggggt tcggatcgca
     9721 gaagtaacga atccaggtga agcaaacgtt agaactcatt atgtttccga ggaatttgtg
     9781 gacaaagtat cttttaataa ggaaacgaat acacttgttg tggactttgg taatctagca
     9841 gagggtcaat cgtattttat tgaatatgct cttgaagtga ccgatgtcga cttgttcaat
     9901 gaaggaagac aagaggatgg gttttctcgg aatcgagcga gtatgaccta caataatcag
     9961 gtaatgagcc gagactaccg cttgagaatc cctgaaagta atcttccggc aatctcttta
    10021 agaaaaagtg tggacaaaaa tgttatcaat ctcaatgaaa atgaactaac gtatactctg
    10081 catgtctcga tgttttctgg aacaagaaat acgcttgtta ttgaagatgt tttggaagaa
    10141 ggcatggaat ttgtgtcatt tctatcagat attccctctt tcattgatgc cgaagtcaat
    10201 aatggaatag ttcgtttcag tgcgaataat acgattggtg gtccgacaag ttttaccctc
    10261 tcttttaagg tcaatgtaga ggcgtatgcg gtcggcgatc aaattttaaa ctttgcaacg
    10321 cttgtgaata gtgacaatcg attgaatacg agtactgtaa gaacaaggaa aatcgatggg
    10381 cgtattaaag taattaaaac tgatgagaat ggccaaaggc tggcaggagc aaaatttgaa
    10441 attttatcaa gcgattcaca agtgatacaa gaaggtataa cggcagaaaa cggagaattt
    10501 ttaagtgaac cgcttcctgt aggtagctat caagtccgag aaacagaagc accggaaggg
    10561 tatcttttag atgcgacggt acatgatgtt tttataaatg aatactccat agaagaagtg
    10621 acgttaacca ttgaaaacat ccttgatacg ggtggtgttg aactaattaa acatgctgaa
    10681 ggcagtggtg aagtcct
//
LOCUS       DACEOE010000085        10614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEOE010000085 DACEOE010000000
VERSION     DACEOE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.087"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..2714)
                     /locus_tag="IW774_001770"
     CDS             complement(3..2714)
                     /locus_tag="IW774_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1154116.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLNKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(2724..3425)
                     /locus_tag="IW774_001771"
     CDS             complement(2724..3425)
                     /locus_tag="IW774_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154117.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            3611..4210
                     /locus_tag="IW774_001772"
     CDS             3611..4210
                     /locus_tag="IW774_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1154118.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4291..5304
                     /locus_tag="IW774_001773"
     CDS             4291..5304
                     /locus_tag="IW774_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1154119.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            5412..6329
                     /gene="rbsK"
                     /locus_tag="IW774_001774"
     CDS             5412..6329
                     /gene="rbsK"
                     /locus_tag="IW774_001774"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1154120.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            6357..6752
                     /gene="rbsD"
                     /locus_tag="IW774_001775"
     CDS             6357..6752
                     /gene="rbsD"
                     /locus_tag="IW774_001775"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1154121.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            6752..8125
                     /locus_tag="IW774_001776"
     CDS             6752..8125
                     /locus_tag="IW774_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1154122.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            8482..>10614
                     /locus_tag="IW774_001777"
     CDS             8482..>10614
                     /locus_tag="IW774_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1154123.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLP"
BASE COUNT         3281 a         2127 c         2033 g         3173 t
ORIGIN      
        1 ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt gaaatgggta
       61 acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac ataagcccac
      121 catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa gatgaagtca
      181 gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa gatgatattt
      241 tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt tattgtagat
      301 aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac gataaaagcc
      361 agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc cattgattct
      421 tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc tgtggcaagc
      481 agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg cagcaaaaat
      541 gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga catataaata
      601 aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc tttttgatct
      661 tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc tactttttgc
      721 gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc agttaaatgg
      781 ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact ttgcgaagca
      841 tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc atttactttt
      901 tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga tgaatccaat
      961 gtaacgattg cttgatagtc aatgatcgtt ccaaattgtt tggctgaaac agaacttaga
     1021 gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat tccgataatc
     1081 gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt ttgattaaag
     1141 ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc tggcttcggt
     1201 gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa aacgatcatt
     1261 gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg cggccagtca
     1321 taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa aatgatcctt
     1381 ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga ggctagactt
     1441 gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc ctttaatgtc
     1501 ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt gatcaatgcg
     1561 gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg atcagacaga
     1621 tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta tgatggttta
     1681 tttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga ggccagttca
     1741 cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg ttgagataat
     1801 gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc tgactgttgc
     1861 gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg tgctttgcca
     1921 tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt cagctcttct
     1981 aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt ttgctccatt
     2041 ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa atcaagatat
     2101 aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa ataatcgacg
     2161 ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa tggagaacgg
     2221 acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat ctgataaata
     2281 tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg gtttggtttt
     2341 tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt gttataagaa
     2401 aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt tcctgtttct
     2461 acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa gccaccagta
     2521 gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat cgttttttgc
     2581 atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat gacaataagg
     2641 atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc tttaagatat
     2701 gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga aggatgatca
     2761 tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc agcgatgggc
     2821 gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct gctagcgttt
     2881 tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt cggttcatca
     2941 cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct ctgctgttcg
     3001 cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac ttgcctcaat
     3061 acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac attttctttt
     3121 gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc tgttcgtcgg
     3181 taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat aatgatctga
     3241 ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt tcctgctcca
     3301 cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat atcgtcattt
     3361 gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac ttcgacatac
     3421 ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat cgaatgcgtt
     3481 ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg aatcctttaa
     3541 tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt tatttgaaag
     3601 aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa aaactgcttt
     3661 tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc cacacactgc
     3721 agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta ccgctgcctt
     3781 gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac aaactgatat
     3841 gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga aggattcttt
     3901 gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt tttttggtac
     3961 ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa gcggtgtagc
     4021 gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt accatatcgt
     4081 gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt tcataaaaaa
     4141 gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag ctatccaaca
     4201 aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt catgggaaaa
     4261 taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac aatcaaagaa
     4321 gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa tggaaatacg
     4381 gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga attaggctat
     4441 cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat tggtgtactc
     4501 gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga ggatatcttg
     4561 tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca aaaagaaatc
     4621 gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc gacttctgct
     4681 gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt cattgttttg
     4741 gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt tcgcggtggt
     4801 tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct tgtctatcct
     4861 gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc tcttgatgtt
     4921 taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa acaaggtggc
     4981 taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc tttaaatgat
     5041 gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat ccctgaagac
     5101 tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc gaaactgaca
     5161 acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt gttagatcgg
     5221 atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt agaaaaaaga
     5281 ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc gaactattaa
     5341 aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg agatagttgg
     5401 aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac actacccttc
     5461 gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt tttcacgctg
     5521 gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca cggacatctt
     5581 ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta ctgacaaaag
     5641 aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga caagcaatga
     5701 ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca aataatgctt
     5761 tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac tttattattg
     5821 ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca aaagacgcag
     5881 gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact gaactacttg
     5941 aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca ggaatcagag
     6001 tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta ggtattggaa
     6061 cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa gaacacggta
     6121 tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat acatttattg
     6181 gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg atcctttatg
     6241 gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct atcccttacc
     6301 gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa acaaacatga
     6361 aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg gggcactttg
     6421 ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa aaaatagatt
     6481 tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt ttagaagaac
     6541 ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg gaaatattgg
     6601 aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat gaagaaatga
     6661 aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca ccttacgcca
     6721 atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc ctgaacagat
     6781 ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag acaatattga
     6841 ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc cagtagccaa
     6901 tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac tcgatagata
     6961 tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga cgcgtcaaat
     7021 cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg gtttgatatc
     7081 ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa aaggtctggt
     7141 tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc atattttgtc
     7201 tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt ctgctgccaa
     7261 aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga ttgaaccgaa
     7321 tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg ggattcctgt
     7381 agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt tcttttattc
     7441 ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag tatttgtacc
     7501 tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc ctgcgggtgt
     7561 ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta acggaccgat
     7621 cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta tccaatgggc
     7681 tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa tcgttccgtt
     7741 gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc tgcgaagtaa
     7801 acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac ttgcgggagt
     7861 gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg ttgctgcttg
     7921 cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag cgatcgcttt
     7981 tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt tctggtactt
     8041 agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta gcgggtttga
     8101 tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag atgtttatca
     8161 catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt gatactttcc
     8221 aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt tatccccaaa
     8281 caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg ctcgttgctt
     8341 atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa aagcttttcg
     8401 acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga aaatcaatga
     8461 aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct agctgctact
     8521 tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga taatcaaata
     8581 gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag tgaaacaacg
     8641 gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc gaccgagcca
     8701 actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac atattccctt
     8761 acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc ggattcccct
     8821 gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt agcggaaacg
     8881 aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt cgctgttaat
     8941 ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa caatttatct
     9001 attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac tgatcctagt
     9061 gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt gggtaataac
     9121 aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa cagcgggaat
     9181 tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca gagagaacca
     9241 aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc tcgtccaaat
     9301 acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt tgttgtaaaa
     9361 tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc aatcgttcga
     9421 gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc aaatactttt
     9481 gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta tatcggtggc
     9541 tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc ccaacctggc
     9601 gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat ctatattggg
     9661 aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac ttctatttca
     9721 gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc tgcttcaggt
     9781 tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca tttcagtcaa
     9841 aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga aatcgggcaa
     9901 aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag tggatctgga
     9961 aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg atcatataaa
    10021 ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag ctaccaaatc
    10081 cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta tgccgacttg
    10141 atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa gtcggaaaca
    10201 aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga tcgccaatat
    10261 tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga tgaaccgaaa
    10321 aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc taagcgattc
    10381 gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga attttcaacg
    10441 ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg ggatttgtgg
    10501 aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa agctgcaact
    10561 gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct gcct
//
LOCUS       DACEOE010000086        10595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEOE010000086 DACEOE010000000
VERSION     DACEOE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.088"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(777..2408)
                     /locus_tag="IW774_001778"
     CDS             complement(777..2408)
                     /locus_tag="IW774_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1154124.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2422..4095)
                     /locus_tag="IW774_001779"
     CDS             complement(2422..4095)
                     /locus_tag="IW774_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1154125.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4108..4425)
                     /locus_tag="IW774_001780"
     CDS             complement(4108..4425)
                     /locus_tag="IW774_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1154126.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="IW774_001781"
     CDS             complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="IW774_001781"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1154127.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6087..6872
                     /locus_tag="IW774_001782"
     CDS             6087..6872
                     /locus_tag="IW774_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1154128.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6875..7567
                     /locus_tag="IW774_001783"
     CDS             6875..7567
                     /locus_tag="IW774_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1154129.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7841..8212)
                     /locus_tag="IW774_001784"
     CDS             complement(7841..8212)
                     /locus_tag="IW774_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1154130.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(8612..8842)
                     /locus_tag="IW774_001785"
     CDS             complement(8612..8842)
                     /locus_tag="IW774_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154131.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(8936..9274)
                     /locus_tag="IW774_001786"
     CDS             complement(8936..9274)
                     /locus_tag="IW774_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154132.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(10202..10318)
                     /locus_tag="IW774_001787"
     CDS             complement(10202..10318)
                     /locus_tag="IW774_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1154133.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         3310 a         2217 c         1728 g         3340 t
ORIGIN      
        1 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
       61 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
      121 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
      181 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
      241 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
      301 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
      361 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
      421 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
      481 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
      541 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
      601 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
      661 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
      721 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
      781 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
      841 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
      901 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
      961 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     1021 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     1081 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     1141 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     1201 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     1261 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     1321 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     1381 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     1441 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     1501 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     1561 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     1621 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     1681 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     1741 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     1801 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     1861 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     1921 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     1981 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     2041 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     2101 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     2161 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     2221 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     2281 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     2341 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     2401 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     2461 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     2521 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     2581 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     2641 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     2701 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     2761 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     2821 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     2881 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     2941 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     3001 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     3061 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     3121 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     3181 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     3241 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     3301 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     3361 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     3421 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     3481 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     3541 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     3601 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     3661 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     3721 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     3781 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     3841 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     3901 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     3961 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     4021 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     4081 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     4141 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     4201 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     4261 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     4321 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     4381 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     4441 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     4501 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     4561 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     4621 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     4681 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     4741 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     4801 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     4861 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     4921 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     4981 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     5041 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     5101 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     5161 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     5221 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     5281 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     5341 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     5401 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     5461 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     5521 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     5581 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     5641 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     5701 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     5761 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     5821 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     5881 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     5941 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     6001 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     6061 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     6121 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     6181 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     6241 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     6301 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     6361 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     6421 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     6481 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     6541 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     6601 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     6661 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     6721 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     6781 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     6841 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     6901 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     6961 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     7021 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     7081 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     7141 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     7201 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     7261 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     7321 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     7381 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     7441 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     7501 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     7561 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     7621 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     7681 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     7741 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     7801 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     7861 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     7921 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     7981 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     8041 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     8101 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     8161 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     8221 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     8281 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caacccgttc
     8341 tctgagtgtt gattctataa tatttttaat ttcctatata aatcaaaaat attatagaat
     8401 caataagcta attaagaaac aaactttaga aactctagaa gaacttctga atttatatag
     8461 aagtgtgtag ctacttacta aatatacaaa agttaaaata aaatactctg gatactcttt
     8521 atactgcatt aaagttagta atattaacag caaagatatc gaaaaaacaa taacataata
     8581 tgtgtcaaaa tttttcataa taagaactcc cttactgttg catactaaca taatccaaaa
     8641 actcttctac ttcagctaaa gacattccac tttctttagt ccaaaaaaca gttttttcta
     8701 tcgatccttc tctgtaaact gtaaatgtcg gagtcgagtt aattgagtag cgactcttga
     8761 attctttcca ctcattttca tcttctctaa cttttgctac attcacgtaa taaatatttt
     8821 cttttccctt ttctaccagc atatcgaata gcttcctgct tttgatgatc gatttcttgt
     8881 gctacatcta gtaaattttc atcaatttct atatcataaa gtatttcact agtgtctaca
     8941 tccacaatat atacttttta tcagtagcaa tttttgagta atccgttaaa atgatctcag
     9001 tctcgtcact ttcatcagta taaatatacg ttaacaatga actcaccttt tcaatcggtg
     9061 ttagctctaa gcctactgta ttttttacta ttgagtcatt atttaccacc ataacttcgt
     9121 actgtatata ccatcctatt tggtttccat caatatcagt aattggctca ctagctaatg
     9181 gagtctccgg aacaatattt tcctgatatt tcttaaactc ttctgtagca cgaaaaaggt
     9241 tatcatgttc ctcacttgaa actggttgag tcatagaaaa tgttacttgg ctaaacagag
     9301 ggaaaataaa cactacaatc aacaaagtat aaaaaaaagc ccttcttttt cattttttat
     9361 cttccaatca caaattgaaa aacttattcc tgcaactttg agtctagtat cacaatcgac
     9421 tgactacaac tattcattta cattctactt taagttcctc cttaaccatt aattttatta
     9481 aagatacttg taatagtgat tctcacaaaa tttcatgaaa aatactttat tactgtatct
     9541 aaaattccaa tacacaactt atcgactatt atctgaaaac caaaacttat tattccttga
     9601 tgttattcta gcaatagtaa ctgctttttt atttttttgc atattttttc ggcgttactt
     9661 tttaaagtta actttttcga caaatagaaa gagatacgaa tcactgattt ttttgaagaa
     9721 aagaaatgga gtgagctgaa cgttagagat attgggagct ttcgtaactg cttattaaac
     9781 gataatggtt atttgcaacc gtattgctcg cttctacatg aaatatttca tgaaatattt
     9841 cgcaaaacct ttgattatgt agttacgttt ccattcttga ctaaaaatgt ttcaaaaacg
     9901 aacaaaggtg ttaaaaggta aagtgatcaa tactactggt caaaataaga ggttgaaagg
     9961 gaattgaaaa ataactacag ataatacttt tataaacgta cgtgctttgt ggttctgtag
    10021 ttaactatat aactggaatt agggaatggt atcaatttga aaagaaagat tctaccaatt
    10081 tatcatatgt taaaaatgga aaatcaacag cagttctttc ggaaacctta cacaaaaacc
    10141 gattattgaa aatagccgaa aaacgttttt cggctatttt tttaattaag tttccttctt
    10201 cttacctttt cccactattc cgtttcacat ctttgatgat cttcagcgca cgttttcgtg
    10261 ttttgatatt tgggcttttc aagcggcggt atgcacttga gatatctgtt ttttccatat
    10321 ggacgaccca ctttcttctt tgattgtagt catgctaaaa aacacaaatc gaaacaatta
    10381 cgatttgtgt tttaaatggt atcaaaagtt aaaatgaaag tcaacttgtt aaaacagtcg
    10441 atcagactag ctgattgact cctatctctg gaaataaatt ttcattatta cgttttagct
    10501 acctaaaaaa ggccctcttg tccgttaact ttaatatcta ttgttatagc tagtttacct
    10561 attgaacgta ctgtgtgttc gatagaacac taaaa
//
LOCUS       DACEOE010000087        10446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEOE010000087 DACEOE010000000
VERSION     DACEOE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.089"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(105..1562)
                     /gene="ascB"
                     /locus_tag="IW774_001788"
     CDS             complement(105..1562)
                     /gene="ascB"
                     /locus_tag="IW774_001788"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1154134.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1559..3454)
                     /locus_tag="IW774_001789"
     CDS             complement(1559..3454)
                     /locus_tag="IW774_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1154135.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3512..5287)
                     /locus_tag="IW774_001790"
     CDS             complement(3512..5287)
                     /locus_tag="IW774_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1154136.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(5337..6833)
                     /locus_tag="IW774_001791"
     CDS             complement(5337..6833)
                     /locus_tag="IW774_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1154137.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(7027..8649)
                     /locus_tag="IW774_001792"
     CDS             complement(7027..8649)
                     /locus_tag="IW774_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1154138.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(8977..10290)
                     /locus_tag="IW774_001793"
     CDS             complement(8977..10290)
                     /locus_tag="IW774_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1154139.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         3181 a         2206 c         1685 g         3374 t
ORIGIN      
        1 atgactatgg tgacttgtga tcgaggagtt ggcaggagaa tactgtttat tcgtaaagaa
       61 ggggctgtga caagactttt gtcacagtcc cttatttcct tttattatag gatttcgcca
      121 tttgattgga tgacttgtct gtaccaatca aatgattttt tctttaaccg tttctttgtg
      181 ccattccctt cgtcatcttg atcaacatag atatatccat aacgttttga catttgtgaa
      241 gtagaagcgc tgacgatatc aatgcagccc cacgacgtat aaccaaataa atctactcca
      301 tctttgatag cttccttcat ctgctcaata tgctgcttca aatagtcaat gcggtaatcg
      361 tcttgaacgg ttccatcttc ttctactttg tctatagctc cgagtccgtt ttcagctatg
      421 aacagtggtt tttgatacct atcatataat tgattcaaag caattctcaa acccttagga
      481 tcaatctgcc agccccattc acttgatgga agatacggat ttttaattcc tccagttatt
      541 aagtttcctt ggaccccttc ccaattttct ggatgagcac tgactgcact agacatgtag
      601 tagctaaaag aaacaaaatc aacagggtag gctgctaaga tttcttcatc acctacttct
      661 atttttaagt tgacaccttt ttcttcaaac attcgttgcg tatacgctgg ataatatcct
      721 cttgcttgaa catcactaaa gaacaaagaa gttctttgct gctcaattgt tttttgaata
      781 tcatccggat cacaagaata cggaacaaga agctgatagc tgatcataca accgacttgt
      841 gaacctttga taatttcatg acaagctttt gtagctaaag cacttgctat aaactgatgg
      901 tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt tttcttcaat tactcctacg
      961 ctaacaaaag ggtgatgttt tacacagttg atctcattga aagtcatcca atattttact
     1021 ttatttttat agcgtttgaa tactgtttca gcgaaacgaa cgtaaaactg aataagcttt
     1081 cgattaggcc atccaccata ttctgtaacc aaatataacg gcatttcata atgggaaaga
     1141 gaaacgactg gctcaatccc atattttaga cattcgtcaa acacttgatc ataaaaaaat
     1201 aatcccgctt cattgggtgt ttcttcatca ccatgaggaa aaattcttgt ccatgcgata
     1261 gacatccgat agcatttaaa acccatttca gcaaataaac ggatgtcttc tttaaagtga
     1321 tgatagaaat catttccgtg tcttttagga taataatact tgctgttggg atcctgcgcc
     1381 gcttttactt gctgatgcgt catcatatgc cattgatctg tccatttttc tttaggtaaa
     1441 tcgctgtcga atgtatacac atcagacaca gataatcctt tttcatctac atcccatgca
     1501 ccttcacatt gattggccgc tgtcgctccg ccccataaaa atccttctgg aaattttttc
     1561 atatcgatcc ccctaaactt gctagttttc ttgtttgatt atttttctta tgactagtaa
     1621 tgatattttt gaatctacta ctatttgtaa caataaccat tgtggtcgga tcatatccct
     1681 ctttgattaa ttcttgcaaa tctacttcca tcaataaatc gcctttacgt actttttttc
     1741 catcagatac tgcactttga aagtatttgc catttaaatt cactgtatca ataccgatat
     1801 ggatcagtat ctctgctcct tcttcactga ctaccccaat tgcatgtttc gttggaaaaa
     1861 ctgttgaaac ttccccgttt acagaactgt agatttttcc gtccgttgga atgatcgcaa
     1921 acccttctcc taaagatcca ctagcaaatg ttggatcgtt tacttcagat aaatcaatta
     1981 tttcacctgc tgcaggagta cttaacacta tatcctttga ggattcttct tcgatttcat
     2041 ccttgtagcc gaaaagaaaa gttagcatac aggaactgat catcgctaag aaataactag
     2101 caactagcca aacaaatcct tcaccaatat aaatcgggaa agacaaaata cttcttgtag
     2161 caactgccaa agcaccggct cctgcggcac cagctactcc accaaatata gctccacata
     2221 tacaagcaat gtaaaatggt cgtttatatt taagagtcac accataaaca gcaggttcag
     2281 tgattccaaa taatgctgtg ataaatgcag ataaagataa actttttact ttcttatttt
     2341 tcgttttcaa aaatacccca agtactgctc ctgcttgcgc agctacggca gtcatacatg
     2401 ctggacctag cgtatcacga ccgtagaaag aaagattgtt ggtcattact ggtacaagtc
     2461 cccaatgtaa cccaaaaatc actaacactt gccaaaatcc gccgataaca gcaccagata
     2521 aaacagggct aaagttataa acagctaaat atccagctgc aatcagctga cttgcataat
     2581 ctgcaattgg acctacaact aataaaataa ttgggaccat cacgatgata caaatgaatg
     2641 gtgtcaataa atttcgaata ctttcgtgca agcttttgcg aatcttaggc tcaatataag
     2701 aaagaaccca tacagctaaa attgctggaa taaccgtact tgtatacctt gctagaatca
     2761 cgggcatccc tagaaatcgc aaggttatgc tatcactata tgcactgaca atcgtaggat
     2821 aaacgagagc tgctgcaata accatagcga tgaaacgatc tgtattgaac ttttttgcag
     2881 cagtatatgc caaaaatata ggcaagaaat agaaaaatgc atcagctgct gcattcaaaa
     2941 tgatataagt tcctgattca gtagttaata aattcaaact cgtacataag gcgagtactc
     3001 ctttgatcaa accacttcct gccaagacgc ctaaagtcgg ggcaaaaaca gcggcgatca
     3061 tgtctataaa agagtttaaa atgccttttt tctcagttcc tctctttcca ctagatgcat
     3121 catcttctag tttcatttgt ttggataata cgtcatacac ttcatttact gcatttccta
     3181 ctacgacttg aaacattcct ccagcttcga ctacagtgat cacaccttct agttgctcta
     3241 ccttctcctt atctgccttc ttattatctt ttagggtaaa gcgtaacctt gttgcacaat
     3301 ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg gacaatatct ttcgctaatt
     3361 gtgaatagtc catttttact tcccccttgt taattaatgc cgtagcaatc cttcttacaa
     3421 gataagtata gttcttagaa aacggtttct ccattcaatt agtacccctt ctaatagcga
     3481 acgaaactgt acaatagtgt gtatggttta cttagccgag caaatcactc actgcttgaa
     3541 agaaaacaag tatatcgttt ctcttatagt caaaggagac aaaaaaaaca cgcttgatct
     3601 tcctttgctg atgtataagt ggtatctcaa gctgatactc tgtgattttt gtttctactt
     3661 ggcttttgat aagacagcac aacaaagtct cggcatttaa cggaagcata actaggtcag
     3721 aactaggaga gattagttgc tgaatggaat ctatcttttt atggattttt atttgatttt
     3781 cttctttgta gtaatgatca agcagttttg ttttcttttt ttcttcaact atggtcagct
     3841 tttcacgcaa aatctgataa atctcccaag attcaaactc atctagaaca ctaggtagat
     3901 aaatcaaatc atcccactca aaaataagct cttgctcagt agtcaaaatt gcatcatatt
     3961 ccgttatttt gtttgattga tttttgaaaa gatacactgg ttctatccgg cattctttga
     4021 tccacatgtt catactttgt tgaatctgtt tttttagtgt attcaaaata ctaattggct
     4081 ggttactaac aatcagtatc tccccttgtt ttcgaaaagg attcaacgct tcggcaagat
     4141 acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa ttgaaaatgt attgggaaga
     4201 aacgccgaat taaataaatt tctaaaggat aacgggagat gatcttttgt acaaaatgat
     4261 tcatggtatt atgtccggca tccatcctcg ttttcatttg tttgatatgg gttagcaaaa
     4321 attctgtttc atggaacaat aacgaaacag gaagatctaa aaaacgaata atcgtttgct
     4381 caaatgctct tagattcttc tctatttctt tgtttttttc atcattaatc atcaaataat
     4441 ttaactctaa tagtcgattt tcaattgctt gtttttcttg attggaaaaa gaatagggta
     4501 gttctgatga taactttttt gtgagggtct ctaccatcgg aataaataat gtatacctgt
     4561 ttgttttttc taaaagttct tctcttcgaa gaatggtcaa aacgagatac cgagaaaatc
     4621 gtatgaagtc ctctcctgaa acaatataat tatatgatat aagaacctct tttaatgtct
     4681 tttggacagt cggcatgata ttggaaaaaa ttgtactgat ttgttgaggc aaccttgcgt
     4741 gttggatcac cgtttctgtt ccaacaagag ataagaaaat tctttttgaa ttttcttcag
     4801 caatgatttt tagtcctttt gtcgacgaaa cttccagttc taatttagga ttgcgctcga
     4861 tccaacgcaa aatcgttttt atttgtaatg atacggctgt ttttgaaaga aagaaaatgt
     4921 ctgctatctt ttgcatactg acataccctt tttgaaacaa taagtacgtc acaatagttg
     4981 aagctcggaa atccatgctg gaatagattt tatgtttcgt cacctcttgt atgatttgct
     5041 gtttagtttc agattcaatt gattctaaaa aataacctga acgtttattt gactggataa
     5101 tgattttctt ctcgtagata gcaagcatat ttttgatttc agtttggatc gttttcttgt
     5161 tggtacctaa agcttcagct aattcttcgc ttgttatcca atgattctta tttcttaata
     5221 ctacaagaat ttggagttgt cgtttattta agtccatttg cttacacctc ctctttgtta
     5281 taaacataca ggaaaaacct ctcaaatgac aatagtcact tgcagaggtt taaaaatcat
     5341 ttgaagaaag atctatattc aaggttttta ccattagact ggataatttg tttataatat
     5401 tcaaaactat cttttttcaa tctcctaaag cttccgctcc catcattttg tctatccaca
     5461 taaataaagc catatcgctt cctcatttcc cctgttcctg ccgatacgat atcgattggt
     5521 ccccaccata agtaaccaat gatttggcaa ccatctttta ttgcctcagc aatttcctga
     5581 agatgtattt ttagatactc acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt
     5641 gcttcatcta gtccaatgcc attttcaact atgaataatg gtaattcata acggtctgtc
     5701 aattcattca tgacataacg aaaccctaga ggatcaattg gccaactcca aggttcggga
     5761 gatttttcct tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc tacgcgcatt
     5821 tctgcttctt ttttatagac agcgctacga taataactaa atgcaatata atctgccgtg
     5881 ttagcttgca agcactgttc gtctccatct aacattactg gtgcacagtc ttcttccttc
     5941 catgtcctgt aaatataagc tggataatgc cctcggcaca tgacatcgga gaaaaaccat
     6001 gaattgcgtt taaattgcag tgaaccaaat acattctctg gatcacagtc atatgcataa
     6061 gtcgctaatg cagaaaagct acacataata cccatcttag catttggatc gattttcttt
     6121 aataattgag tagcacgggc gttcgctaga aacatatgat gacaggcttg ataaatcact
     6181 ttttttgtta gtgtagcaat gggttcaagt ttatctctag cagggaatgt aaccaatcca
     6241 gcagctgcat aaggcatacg aaaagcatta ttgatctcat taaaggtcat ccaatatttc
     6301 actttccctt ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat
     6361 tttcgatttt gccacccccc atactctcct accaatgcta agggggtttc ataatgacta
     6421 agagtaatta ctggctcgat atcatatcga tttaatgtat tgattagtct gtcataaaat
     6481 acgagaccag cctcatttgg cttctgttcg tctcctcttg gaaatattcg tgcccatgag
     6541 atgcttgttc gaaaagcttt taaacctaac tccttcagta aacgaatatc ctcttcaaat
     6601 cgatgataaa aatcaatcgc ttcatgagaa agataatgga gtgattcgtc taaagcaatc
     6661 tcccaaatct ttttttcttc attccactga atactaggat gctggttcat gatcccgata
     6721 agcgtatctg tcacattagg taatttgccg tcttccaacc atgcaccttc acattgatta
     6781 gcagctactg ctccgcccca taaaaaattt tctggaaata tttcctgatc cataactatc
     6841 tctcctcgct atcaacaatt tcttacttga agtatagctc gattcatcaa aacaagatat
     6901 tctaactatt tcggcagaag agaagaaaaa aattgaatgc tactcttcat ttttaatatt
     6961 tatatataac aaatacataa gacgatcccc gaaagaaaac actcgttaac catctaagca
     7021 aacgatttat tttacttcca acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc
     7081 tggtaactgt ttcagaggat aatttgacac aataaccctt aaagcttcac gtggaatagt
     7141 gaacgtctgc ttttcactgg acatattaca aataacgagc cattgctggt cttcatatat
     7201 gcgagaatag gcaaagacat gctcaggcgt cttttccact aattcatagt ctccccagac
     7261 gatgatgtca tgttttctac gcaaatcgat caatttcttg tacatataaa aaacagaatc
     7321 ttgatctgct aacgcttctt ctgcattgat ttctttataa tttggattga ctcgaagcca
     7381 tggaattcca gtagtaaacc cagcatgttc acgggcgttc cattgcatag gcgtcctagc
     7441 attgtctctt cctttcgcct tgattgattc caagatttcc tcttttgtaa atccagaaga
     7501 aatcctttcg ttatacatat tgattgtctc aatatctttt gcttccgcga tatcatcgat
     7561 tgggcaattc gtcatgccta actcttctcc ttgatagata taaggggtgc ctttcatcat
     7621 atggagcaag atagcaaaca tcttggcact ttcttttcga tacacatcgt catttcccca
     7681 acgagaaaca attcgcggaa gatcgtgatt attccaaaat aaactattcc atccttcatc
     7741 acctaaagat gtttgccatt tggataatac ttttttcagt tcagagatac tcaagggttt
     7801 caaatcccat ttgtctttgc cttcttgctg atctaatcct atgtgttcga attggaagac
     7861 catggataat tcatttcttt taggattgga ataaagttta gcgatttcgg tagttgctcc
     7921 ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa gttgcttgat tcatttcctg
     7981 aagatactca tgcagtttag gtccattact tgtgatttct ttatccggtt ctttgccaat
     8041 catatcgatc acgtccatac gaaagccccc aataccttta tcgatccaga aattcatcat
     8101 ttgataaatc tcttggcgaa cctgtttatt tttccaattc aaatcaggtt gacgcttgct
     8161 atacaagtgc agaaaatatt gccccgtttt ttcatccaac tgccaagcag aaccactgaa
     8221 tattgagcgc aatctattag gctcttttcc attgaccgga tcacgccaaa tataatagtc
     8281 ccgataagga ttttctttat ttttacgggc ctcaatgaac cacggatgtt catcagaagt
     8341 atgattcacc accagatcca tgatgatacg gatatttctt ttctttgctt cttcaatcag
     8401 ttcttccatt tcttccatcg taccgaattc tgccatgatt gcttgataat tactgatatc
     8461 atacccattg tcatcattcg gagactcgta tactggagaa agccaaattg catcaatacc
     8521 taaatacgcc agataatcca acctggaaat aatcccccgc aaatctccaa taccatcttg
     8581 atttgaatct tgaaaacttc ttggatagat ttgataaaca actgaatggt gccaccattt
     8641 ttgttccatg attttcctcc tataaatgga tgtttctatt ttttgcttaa tcatttattt
     8701 tagtctctct cactaaaaat tgtaacgctt tcaaatagct attgcacctt gttatcggta
     8761 acagaataaa acaaaaaaac agctgaggaa attccctcaa ctgttttttg aaaggattga
     8821 tttatgcagg gaagaatgct gatttcacaa cttctttata aacggtgttc actcagctgt
     8881 cccttggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg tttttttctc
     8941 taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca atatccacat
     9001 gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt ttcctctttt
     9061 agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat ctgaaatact
     9121 ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct ctaagtttcc
     9181 ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca atgctggtgc
     9241 agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta acaagaagaa
     9301 aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac cagctgatga
     9361 ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca tagctgttga
     9421 atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga aacctgcttg
     9481 gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca agaatgtcag
     9541 cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg caactaaaaa
     9601 tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa tcaaacctgt
     9661 gaccagacct aaaatcgttg tagctgccat cccgatgaag atgccacctg gtacacgccg
     9721 aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag aaccaacgtt
     9781 gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac cttcgctcag
     9841 accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca aatctgctgg
     9901 gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa aaatcaaaga
     9961 agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta aacaaacaga
    10021 ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat aacgggctaa
    10081 tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa acactgctcc
    10141 ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat aagccattga
    10201 gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca atttttctat
    10261 ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg aacattataa
    10321 ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca tatactaaaa
    10381 caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt
    10441 aaatcc
//
LOCUS       DACEOE010000088        10342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEOE010000088 DACEOE010000000
VERSION     DACEOE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.090"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            154..303
                     /locus_tag="IW774_001794"
     CDS             154..303
                     /locus_tag="IW774_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1154140.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            314..1828
                     /gene="dltA"
                     /locus_tag="IW774_001795"
     CDS             314..1828
                     /gene="dltA"
                     /locus_tag="IW774_001795"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1154141.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1825..3030
                     /gene="dltB"
                     /locus_tag="IW774_001796"
     CDS             1825..3030
                     /gene="dltB"
                     /locus_tag="IW774_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1154142.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3075..3308
                     /gene="dltC"
                     /locus_tag="IW774_001797"
     CDS             3075..3308
                     /gene="dltC"
                     /locus_tag="IW774_001797"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1154143.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3311..4579
                     /gene="dltD"
                     /locus_tag="IW774_001798"
     CDS             3311..4579
                     /gene="dltD"
                     /locus_tag="IW774_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1154144.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4720..5802
                     /locus_tag="IW774_001799"
     CDS             4720..5802
                     /locus_tag="IW774_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1154145.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5802..6605
                     /locus_tag="IW774_001800"
     CDS             5802..6605
                     /locus_tag="IW774_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1154146.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6675..7415
                     /locus_tag="IW774_001801"
     CDS             6675..7415
                     /locus_tag="IW774_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1154147.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7426..8430
                     /locus_tag="IW774_001802"
     CDS             7426..8430
                     /locus_tag="IW774_001802"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1154148.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8475..10319)
                     /locus_tag="IW774_001803"
     CDS             complement(8475..10319)
                     /locus_tag="IW774_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154149.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3329 a         1707 c         2087 g         3219 t
ORIGIN      
        1 tataaagaaa aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg
       61 ttagtaaaaa aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac
      121 actttgtgtg aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa
      181 ttgaattatt ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc
      241 ctgatttatc tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt
      301 taggaggaag agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa
      361 tgaacctgat cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca
      421 agcatcagac gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt
      481 cgtttttggc aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg
      541 ccatgcctat ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg
      601 ggtggctaaa ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt
      661 tcctgtcata acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc
      721 cagaaatagc gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg
      781 agaaccaaaa ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat
      841 ggaagacttt gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga
      901 tttatcggta ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa
      961 taaagcagtt gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa
     1021 tgtgtgggtt tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg
     1081 agaaaatgtg ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac
     1141 tgcagaaagt ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac
     1201 agaagcaact gtagctattt caagtattca gattgaccag catgtactag atagttatga
     1261 ccgactaccg attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa
     1321 acctgtttca aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg
     1381 ttacctgaac aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag
     1441 ttaccataca ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg
     1501 tatggatcag caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta
     1561 tctgctccaa gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa
     1621 agtacagcag ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca
     1681 gttgactaaa tctatcaaag aacaattact agaagttgta atggattata tgattcctca
     1741 gaagttccat tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa
     1801 attaacagct gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg
     1861 ttttattttt ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg
     1921 gaaaaaagat taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt
     1981 ggaggtaatt attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata
     2041 cttgtatggg gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc
     2101 gccgttattt tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat
     2161 ggaaaagcat cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa
     2221 atgatcatgg aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag
     2281 tttctgctgt tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa
     2341 aaagatcagc tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc
     2401 caaaatattt ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta
     2461 ctgcttcctg tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta
     2521 ggttatatgt acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt
     2581 gcagtcggta caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt
     2641 ttatcatgga atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc
     2701 cgtgattatg tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc
     2761 aagatcgtaa tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat
     2821 ggattgacgt ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca
     2881 gatgcatgga tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg
     2941 catggcttag ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca
     3001 ggatttttag ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa
     3061 aggagaaaag gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag
     3121 atgaagtaag aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag
     3181 catctgttca attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg
     3241 aattcgagcg agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga
     3301 agggttgagt atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg
     3361 cttactgttt gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat
     3421 tagacgtgcc tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta
     3481 tgcattatct gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag
     3541 tccatttcat ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct
     3601 gggagcaccc gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc
     3661 tatgaaaaac aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt
     3721 aaaaactgat tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc
     3781 aatgaagaaa gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa
     3841 agtaaaagag aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc
     3901 tttacctgaa tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga
     3961 agtattcagc aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg
     4021 attaccaaaa caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg
     4081 agaaacgacg aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc
     4141 ccacgagcca gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt
     4201 ttctgatttt cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat
     4261 catcccaccg gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca
     4321 aggatttgcc aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga
     4381 ttttgtcaat caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa
     4441 aggctggttg gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc
     4501 tagtaagtat catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga
     4561 taaactacaa ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt
     4621 gaatattctg aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg
     4681 gtataatcac aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag
     4741 ctacttattg atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa
     4801 gtattcagag aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt
     4861 gtgattgcca aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac
     4921 gaagttggtt ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt
     4981 ggaggttggt gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg
     5041 aaaatcgtcc acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga
     5101 aataaacctt atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa
     5161 gccaaagagt ggggcatacg tcctggagat atggtcactc catatattga atacaaacga
     5221 atgaatggca gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt
     5281 tctctgcgag tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga
     5341 agcgatgtgc aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat
     5401 cccgatattg cttttgcttt agatactggt acagcaggag acactccagg aatgacacca
     5461 aaagaagcag attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg
     5521 atcccgcata aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca
     5581 ttccaatata cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga
     5641 gatggtgttc cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca
     5701 gtgattcatg aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg
     5761 cttgatagtg aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta
     5821 tttctgaata tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt
     5881 ggcttgtttt cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca
     5941 gtccttttcc ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac
     6001 ttttattcgc attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca
     6061 gaccaaaaag taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg
     6121 gcatcaattt gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca
     6181 tgtttttaat cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac
     6241 ctacgggtgt catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga
     6301 tgatttttgt ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat
     6361 ggacaaagtt cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt
     6421 tattgaaaga tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt
     6481 tgctaggagc aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt
     6541 cagttgcaga attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta
     6601 cttagcatgt aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg
     6661 agggggaatg tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa
     6721 atcaagggac aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag
     6781 gtagtgctac gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga
     6841 tccttagcca ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc
     6901 aacttcatcc taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt
     6961 ccattcactt tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag
     7021 ctgaagaatt aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag
     7081 tatgttacgc catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg
     7141 attcaggtta tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag
     7201 atacctatct ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg
     7261 gagaacttgc gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg
     7321 gtgatttaga acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag
     7381 aacttgctgc agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca
     7441 aatgaaaacc acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg
     7501 cctttagatg aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga
     7561 aaccaaaata cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt
     7621 gtccgtcgct taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt
     7681 tttatcatgc ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc
     7741 atccaagcac ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta
     7801 gtcattgatg gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt
     7861 gctcatggaa caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta
     7921 aaaaaagaca aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc
     7981 aaaccatttc ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc
     8041 ttgttgaaaa ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat
     8101 gaagattgga aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat
     8161 tacgggaagt ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt
     8221 gaagtccatt tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc
     8281 ttcggactag gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa
     8341 gcatctctat caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa
     8401 ccagaagaat tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg
     8461 gattccgcta ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga
     8521 tcaatgaaag tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc
     8581 atcatcatta agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat
     8641 aggtttgtat cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg
     8701 tgtatcgaca aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag
     8761 gaatgctcta aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct
     8821 tcgggaagat ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa
     8881 cgcgctaaga taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt
     8941 tttgcttctt ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt
     9001 aaatcttgca gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg
     9061 atatgctgtc gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa
     9121 tctacttgtc tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata
     9181 ataacgatta gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata
     9241 cctaatgccg tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt
     9301 gtacaagcta cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg
     9361 actaaagtgc ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca
     9421 ggagtaaaag ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa
     9481 attccgtacc attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct
     9541 gacgttaaga taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata
     9601 ccacttaaaa tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag
     9661 agaataagtg agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag
     9721 gtccctatag cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa
     9781 taaataaaac taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca
     9841 tagttctgat aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt
     9901 aaatctatca cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg
     9961 tataaaatct tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa
    10021 tttgcttcgt ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc
    10081 acggtaggaa tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta
    10141 tagaaaaccc acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata
    10201 aaataaaaga aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc
    10261 cactttatac gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata
    10321 aaggtattcc tttctaaaac tt
//
LOCUS       DACEOE010000089        10169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEOE010000089 DACEOE010000000
VERSION     DACEOE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.091"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            130..963
                     /locus_tag="IW774_001804"
     CDS             130..963
                     /locus_tag="IW774_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1154150.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            979..1716
                     /locus_tag="IW774_001805"
     CDS             979..1716
                     /locus_tag="IW774_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1154151.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(1871..2839)
                     /locus_tag="IW774_001806"
     CDS             complement(1871..2839)
                     /locus_tag="IW774_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1154152.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            3071..3904
                     /locus_tag="IW774_001807"
     CDS             3071..3904
                     /locus_tag="IW774_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1154153.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            3938..4777
                     /locus_tag="IW774_001808"
     CDS             3938..4777
                     /locus_tag="IW774_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1154154.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            4803..5363
                     /locus_tag="IW774_001809"
     CDS             4803..5363
                     /locus_tag="IW774_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1154155.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            5562..7283
                     /locus_tag="IW774_001810"
     CDS             5562..7283
                     /locus_tag="IW774_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1154156.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            7448..8590
                     /locus_tag="IW774_001811"
     CDS             7448..8590
                     /locus_tag="IW774_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1154157.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            8606..9817
                     /gene="thiI"
                     /locus_tag="IW774_001812"
     CDS             8606..9817
                     /gene="thiI"
                     /locus_tag="IW774_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1154158.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         3477 a         1713 c         2132 g         2847 t
ORIGIN      
        1 agtccattcc ctgtttttta tggtccctgt gctataattc taaataaagg ataaggattg
       61 gaaataatcc ttttatcatt aaaaatatga tgacgaagta gaataaaatt gggagtaggg
      121 gggaaagtca tggatatcca aactgtttta tctgatctga aattaaatgg taaagtgatt
      181 ccagttgttg gtggcgatgt taatcagacc tatcgtataa aaaccgaaca tcgtgcttat
      241 tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg aagcagaagt agacggatta
      301 aaagaactgt cagcattcgt acgtgtaccg gatacgtata tgctaggtga aacaagtgaa
      361 ggcgcttatt tgttaatgga atggatcgag ccgggaaaag gggatcaacg agatcttgct
      421 gcagctttag ctaatctcca tcagcaaaca gcgccacaat ttggttttcg taaagataac
      481 tatttgggta cacttgttca gaaaaattct tttgaagaag attggtggac ttttttcttt
      541 aaagatcggc ttgaatccca gatttctttg gcagaagaaa cgaatcgatg gaatgtacag
      601 cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga aatcggttga accaaaaaag
      661 atcactccac gcctcttgca cggagacctg tggagtggta atgtgttctt tgaccagcaa
      721 gggcatccag tttttgtcga cccagctgtt tcttatggga atcgggaaca agatattgca
      781 atgagccagt tattcggtgg tttccgaccg gaatttttag atgcttatca aactatattt
      841 ccattagaaa agggatggaa agaccgactg ccgatttacc agttatatta tcttttggca
      901 catttgaata tgtttggaga aagttacggt tcccaagtag atcaattact agaaaatttt
      961 taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa agcatgaagt ttcatattat
     1021 gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt taggagtagt tatcaaaacg
     1081 tctgaagatc aaagcgacca gttgggaaga ggcagtgaat tcgtcaatag ctttggactt
     1141 acttgggtca tcacgaatta ttccatggag attaatcgtc tccctagagt cggtgaagta
     1201 atctcagtaa cgactcaagc catggagtac aataagtttt tctgttatcg gaacttctgg
     1261 atccatgatg aagatgggaa agaattagtc aaaatagaat ctgttttcgt attgatggat
     1321 tttgtgaatc gaaaaatgag cagtgttaat gaagagatca ttgctccttt tgagagtgag
     1381 aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga ttgattcggg cttcatgttg
     1441 ccatatcgtg ttcgttttta tgatatcgat agtaatcagc atgttaataa tgcgatgtat
     1501 tttaattgga tcattgacgt attaggatat gactttttga ctacgcatgt tccagaaaaa
     1561 gtaattattc gatttgataa agaagtagag tacgggaatg agatcgaaag ccattttgaa
     1621 caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa tccgaatgca agaacagctg
     1681 tattgtgaag caaatattat atggaaaaat aaataaaaaa gaggttgtga cagaaggggg
     1741 caacttcaag aaataagaag aaatttacga aaattgtttc tcaaattttt gtaaattttc
     1801 gacttagttt tcgaagaagc tatttttgtc acagcctctc tttttaagtt tagctattag
     1861 ataatgatgt ttagttttgg ataaacgaaa tcaattcttc acccgacaaa ccatagttta
     1921 cagcaatcaa tagttttaaa cgtgcttttt gactattaat actgttacag aaaataatcc
     1981 ccattttttc tagttctttt cccccgcctt cataatcata aacaggctct gcgattccat
     2041 tgaaacaacg agaaacaagt actacaggga ttttttggac aagaagcttt tccaaagcag
     2101 ctaaggcttg tggagggaga ttccccgcac ctaaagcttc gattaccaaa ccgtctactt
     2161 tcgtatgggc gatggcttcc aataagtccc cttgcatccc agcatatgct ttaacgatcg
     2221 ggatagtccc atctacagcg gaaatgtcca gtcgctttgt ttctaaaagt tcctgcaaga
     2281 ataaaattcg attcttagtt actaagccga tgggacccaa tgtaggtgtt cggaaagtag
     2341 caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga atggatctca tcgttcatga
     2401 ccaccaaaac ccccttatct agcgcttctt cacaacttgc gacacgaatc gcactttcaa
     2461 aattataaag gccgtcactt ccaagttcat tacttgaacg cattgcccca gtcaaaacaa
     2521 ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc agtttcttct aatgtatctg
     2581 taccgtgtgt gatcaccact ccggatatac ctgatgcata agcggacaag atacgttctt
     2641 ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg aagattgaag atatcttcaa
     2701 ctaataattc tacatgcttg gggattgtga tttctgcatc taataatggg tttgccacat
     2761 caggactaac accgcccgtg agcatgtttt ctttcatggc gatcgttccg ccggtgtgta
     2821 gtacaagtat ttttttcatt ttacagactt ccttacgaat aattttgcat agccgttaaa
     2881 tcttattgta tactatataa aagagttttg ctatcagtga agaataacga atagcctaac
     2941 ttatcataaa aaagctgacc ttattaaaat ggaaaagact caaccttgac ctcctaatac
     3001 aatggagtat ctggttgagt ttcttttaca agaaagcagc caaggaaatc aatcacgaag
     3061 gtggccggaa atgatcagaa tcggattaac atcgtttaaa gaacatgaca aattaactgg
     3121 taaaaagaat aacactcttt atgaatatgc agggtttcta cccttagtgg aattagatac
     3181 tgcctattat ggaattcccc gaaagtcctc agttgagaat tggataagtg aagtacctga
     3241 atcatttcgc tttgtagtca aagtctatca aggaatcagc ggtcaaggag actggaagcc
     3301 ttattatcag accgaagaag agatgattgc gcatttttta gatgttatgt cgccaatggt
     3361 agatgctgga aagatgtttt gctttttagt ccaattcccc tcatcattca aatgtacgaa
     3421 agaaaatgtc cagtacttgc gcaaacttag aaagtggttt gagggttatc ctgtttctat
     3481 agaattgcgg gattattctt ggtacagcga tgaatttctt gaacgtacaa gaaactttat
     3541 gtctagagcc gctttttctt tagccatcat cgatgagcca cagttgctga atacaacgat
     3601 tccttttgat ccatttgtca ccaatccgaa ctttgctctt tttcgattcc atggtaggaa
     3661 tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa aaaagaacgt tatatcgtta
     3721 ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa gtagttgatt ctaagacaaa
     3781 agaggtaggt gtgattttta ataataattc tggtggtgat gcggctgaaa atgcattagc
     3841 actgcaaaaa atattaaata tcaagccaga tggactcaat ccaacccaaa tggatctgtt
     3901 ttaagggctt attttattca agaaaggggc gaagtctatg atcaaagcta ttttttttga
     3961 tattgacgga acattggtca ccaatcgttc aaaagcgttg gaatcaacaa aacaagcaat
     4021 tgaatatgct cggaaaaacg gcattttatg cggtgtagca acggggcgca gcccagtaaa
     4081 gatcaaagaa atcattgatg aattggaatt agatatgtat gtcgtgtata atggtcaact
     4141 ggtttttact gcagatcgca cgattatcga ccaccctttt gaacaaaaag tattggaaca
     4201 tattgttgaa tttgcagatg agaaccatcg acaaatcgtt tttggtgcga gaaatcggtt
     4261 agatggatca acgacgatgc tattaggaca atcgattttt atcaagcgac tagtcagctt
     4321 tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc ctccaggttt ttagccctca
     4381 ccgacaaaag gatcgttatg aaacgttaga gatattaaaa gaaccaattt atcaatgtat
     4441 attgctcagt ccggagtcag aacagaaaaa gttagaacat cgctttcccg agtgtacgtt
     4501 tcagcgttca aatacgtata ctgtagatat catcccaaaa ggtggttcaa aattactagg
     4561 catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa gaaattatgg cattcggcga
     4621 tcattacaat gatatcgaga tgctgaaagg tgtaggaatt ggagtggcca tggggaatgc
     4681 tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa tcaaatgaag aagatgggat
     4741 atacgaagga ctgaaacact tcggtctgat ttattagtca atgaaagaag gaaaaaatca
     4801 gtatggaagc agagacacct tttcaaaaag tagaaacatt ccacaaaact tttgatccaa
     4861 gaagaccaga gatacccacg gcttttacag aaaatgaagc tcatacacgt gctgcgttca
     4921 aaatagaaga acttgttgaa tttgttgcag ctacttcacc taataaagaa caactgcatg
     4981 aagctgttat gctgctgcac gaagcattag ataaagcaga agaaaaaata cttggtaaaa
     5041 cacagtgggg aagtgatccg ctggttgaac aagtagatgc actgacagat atcctttact
     5101 ttacttatgg ttctttttca ttactaggaa tagtgcctga tcgaatattc gaaatcgtcc
     5161 atcaggcaaa tatggggaaa ttatttccag atggaaaacc tcattatgac cctgtaacaa
     5221 ataaagtgtt gaaacctgaa aactggcaga tggaatatgc tccagaacct aaaatcgcgg
     5281 cagaaatcca acgtcagatt caagaagcga ttgctaagaa gaaatccgct gatataggca
     5341 tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt ttgttatctt tatacgatgg
     5401 aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa aatagtatgc tataatctag
     5461 aatagatgtc ttttgcacga gagacgtatt atttggattt atgccaagca gaacgccatc
     5521 tgttgcataa aagatgcata ctgaaaacgg aggaaccata aatgggaaat aatgttatca
     5581 tcggaatcgt cattgcaatc attatagtcg ccattgtcgt ctttttggtt agttacttga
     5641 tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa acgcaaggaa gaattatttg
     5701 acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat gcatctggtt gggcaaagcc
     5761 aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat atctacttcc tcgttcgctg
     5821 aactagaaag tcaaattttc gaagtggaaa acctcaatga aagttttcgg ttcttaaaag
     5881 gaaaaaaagc agttgttgaa gcagaagcta caatggacca aatggagaaa caagtgacgg
     5941 aaatccgtga aggtctaaag gaattgcggg aaacggaaga acgcaactca ctagctgttc
     6001 agcaggcact tgacgtctat gaagaactga aagaaaaagt cagagaagac gagcatgaat
     6061 acggaccagc tctttttgaa ttgcaaaaac agatcaaagt gatcgaaaaa gaatttacgc
     6121 agtttgtgac tttaaacact tctggagacc ctgttgaagc ccgcgaggtt ttggataaag
     6181 cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa aattccaccg ctgtatgagg
     6241 agctccatac cactttccct gaacaacttg aagaaatcag tgatacttac gagaaattga
     6301 tggaagaaca atatgtattc ccagaagaag atcttgctga agatatcgca aaagtaagtc
     6361 gaagaattga aaattcatta gccaatcttg aaaaaacaga agttgaaacg gtcgaatttg
     6421 aaaaccgtga aacagctgat ttgattgatt cgttatatga tatcttagaa cgggaaatgg
     6481 aagctcagag atatgtaaaa actaaccaat caacgattgc agagtatatc aaacatacaa
     6541 caaagaataa ccgtcaatta ttgattgagc tggatcacac atctcaaagc tatacattga
     6601 accacaatga aattggccgt gtacgaggtt tccaaaccga agtggaagaa atggaacgtc
     6661 aaaatgaaca gatgattcca cagatccgtc aacacgaaat tccttattca gaagtgcgca
     6721 cgttctataa aacagtcttc aaagtcttag aagatatcga aaagcaacaa gtagagatcg
     6781 acgactcatt acatgaattg agaaaaggcg aaaaagaagc gcaagaaaag atcgatacat
     6841 tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa acaacgtttg cctggcttgc
     6901 cgaatgatta cctagaattc ttttttgttg ctactgaccg tattgaagaa ctaagtgttg
     6961 tcttgaataa aattcgagta aacatggaag aggtcaatcg tttagttgct ctatgtgaag
     7021 aagatctaga attattagat aagaagacac atgatttagt agatgcagca gcattaacgg
     7081 agcaaatgct ccaatatgca aatcgttatc gtcatacaca cgaagaagtg agagaagcaa
     7141 ttgataaaag ctactattta ttcaataaag aatatcacta tcaagaagca ttagacgaaa
     7201 tcggtacagc attagagcga gtagaacctg gtgctttcaa acgcatcgaa gatttttatt
     7261 ttaatcatcc agacttagta taaagcaaaa aagttagtaa accagcaaga gcaagacgga
     7321 ttattgtctt gttcttgtct ttttttgtat atttgaaaat tgaaacaagt gaaaatttca
     7381 cctgctcttg aaagcacctt ggacaaccgt tataatagaa aagaatttca aaaaagaggg
     7441 gtttcttatg atatattttg ataacagtgc aacaacccca atctttccgc aaagcttaga
     7501 tgcatatgta aaaaccagtc agcggattat tgggaatcca tcaagcttgc atgatttagg
     7561 caatcaagct aaccggttgt tgcagcaagc aagaaagcag atcgctgatt tgatccatgt
     7621 agaaccagaa gaaatctatt ttacttcagg cggaacagaa ggagataact gggtattaaa
     7681 aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg atcatttcag gagtcgaaca
     7741 tccggccgtc agcgagacag cagaacaatt aaaagaatta ggaatcgaag taagtatcgc
     7801 cccagtggat aaacgcggat ttgtacgtgt cgacgaattg aaagaattaa taagaaaaga
     7861 gacagtatta gtctctgtta tggctgtgaa taatgaagta ggatcgattc agcccatcca
     7921 ggaaatcagt gatcttttgg atgattttcc taaaattcat ttccatgtag atgctgttca
     7981 ggcgattgga aaagtaccat tctcgcaatg gttgacagaa agagttgatt ttgcaacatt
     8041 ttcagctcat aaattccatg gcccacgggg aactggtttc atttattgga aaaagggtcg
     8101 tcgtttagca ccattactga caggtggagg gcaagaaaga aatcagcgaa gtgggacaga
     8161 aaatgttcca ggtattgtgg ctatggttaa agctttacgt ttatcattag agataaagga
     8221 acaaaaacct gagcagacac aacgcttaaa agtagctcta atcaatgcgt tgaagacgta
     8281 tgagaaagtg acgatttttt cagaaggaac tgactatgca ccgcatattt tatgttttgc
     8341 tctaaaagga attcgcggcg aagttctcgt ccatgcctta gaagaaaaac aaatatttgt
     8401 ttctactact agtgcctgct cctcacgtaa acatatggca agcagtacat tgcacgcaat
     8461 gcatgtacca aatgatctgg ctacatctgc tgttcgaatc agcttagatg aaaacaattc
     8521 gcttgcagaa gtcgagcaat ttatgattat tttcaagcag ctttataaga aattttctgc
     8581 aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa attatggtcc gttatgggga
     8641 actttcgaca aaaggaaaga atcgaaaaag ctttatcatg caactagcac aaaatgtcag
     8701 acaagctttg aaagactttc cagaaatcaa aatccatgct gatcgggacc ggatgcatct
     8761 tttattgaat ggcgcagacg ggcagctagt tattccaaaa ctagcaaaaa tatttgggat
     8821 ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc gaagttttga aacaagctgt
     8881 tcaagacatc atgaaagaaa tttatactgg gaaagaaaca tttaaaatta tcgctaaaag
     8941 aagcgatcac caatttgaat tgacaagtaa tgaattgaat caaacattag gtaatgctgt
     9001 ttttgatatt ttcccgcata tccaagttca aatgcgtcaa ccagatattc cattacgtgt
     9061 agagattcgg cgtgatggcg cttatctatc gtatgaaacg atcaagggag ctggaggatt
     9121 gcccgtagga acaagcggaa aaggaatgct tatgctttct ggggggattg attctcctgt
     9181 agcagggtac tttgccatga agcggggagt ggaaattgaa gctgttcatt ttgctagtcc
     9241 gccgtataca agtgaacaag cactgcaaaa agcaaaagat ctaacagcca aactagcacc
     9301 ttatgtggga acgatccatt ttatcgaagt gccatttacc gagattcaag aagaaatcaa
     9361 aaagtcagtg ccacaaggat attggatgac aattactcgt cgaatgatgt tgcgtttgac
     9421 cgatgaaatt cgccgtattc gtcatggttt agtcattttg aatggtgaat cattaggtca
     9481 agtggcttca caaactttgc aaagtatggt ggctatcaat gaagtaacga atacgccaat
     9541 cattcggcct gtagcgacaa tggataaact tgaaattatt gaagtagcag aacagatcga
     9601 tacatttaat ctttctatcc agccttttga agattgctgt acgatttttg ctcctccaca
     9661 gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat gaagagcgtt tagcaataga
     9721 tgaaatgatc caacgagcac tagctggctt gaagatggaa gagatacaag cagaatcgtc
     9781 taatgaaaaa actgaagaat tttcagaatt gctataagag gttatgaaaa tagtgttttg
     9841 accagagtat tagagaaaca aaacgaaaaa atagctccat gtattttacc gtttttgaac
     9901 taatgtcgaa gattagctga ttgaatacca tttatcaaga agttatggaa aagccggttc
     9961 ttttttacta actgaaaaat aaaattttat ttagagatca agctacagtt gataaatgat
    10021 ataaaaaaga gaatttccca tgaaatagcg aggttgtgac agaagtgggc agcttcaaga
    10081 aataagaagg aatttacgaa aattgctttt caaatttttg tgaaatttcg gcttattttt
    10141 cgaagaagtt acttctggaa caccgtgta
//
LOCUS       DACEOE010000090        10093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEOE010000090 DACEOE010000000
VERSION     DACEOE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.092"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            318..2153
                     /gene="tdc"
                     /locus_tag="IW774_001813"
     CDS             318..2153
                     /gene="tdc"
                     /locus_tag="IW774_001813"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1154159.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2458..3936
                     /locus_tag="IW774_001814"
     CDS             2458..3936
                     /locus_tag="IW774_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1154160.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3902..5233
                     /locus_tag="IW774_001815"
     CDS             3902..5233
                     /locus_tag="IW774_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1154161.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5347..7899
                     /locus_tag="IW774_001816"
     CDS             5347..7899
                     /locus_tag="IW774_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1154162.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7906..9345)
                     /locus_tag="IW774_001817"
     CDS             complement(7906..9345)
                     /locus_tag="IW774_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1154163.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9691..10053
                     /locus_tag="IW774_001818"
     CDS             9691..10053
                     /locus_tag="IW774_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154164.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3219 a         1652 c         2137 g         3085 t
ORIGIN      
        1 ttaaaaaata aattgcgaat ttttaacaat ataaaaaaat aaaaatatgt ttagtgaatg
       61 aaggacagtc attttatttg tttgttcgct ttttttaggt tatctatttt agaagactga
      121 tttattgaaa aattgttcgc ttttttgcgt tgaattatca gaaaaatccg actttattta
      181 tttttgtttg taaaaatcta cgtggatttg atgttataaa aaaaaaattc tcatttttgg
      241 cgctatagtg aagaggtgtt acagataaac acatgtatct gcaagacatt gaccaacaaa
      301 atatggaggg aagaaaaatg aaagatatgg atatcaaggc cgtctttata ggcgacaaag
      361 ctgaaaatgg accagtttat aaaatgttgt tgaacaagat ggtagatgaa catcttggat
      421 ggagagaaaa ttatattcct tcagatatgc ctgcaattag cgaaggagat aagctcactc
      481 cagattatct agccacccgc gaccatatga tagaggtctt ggatgaagtc agtcagcgat
      541 tacgcgcagg atcgatccca tggcattcag caggccgtta ctggggacaa atgaatgctg
      601 aaacattgat gcctgcttta ttagcttaca actatgcgat gctgtggaac ccaaataatg
      661 ttgcattgga atcttctatg gcaacttcgc aaatggaagc agaagtagga caggattttg
      721 cttcattatt caatatgaca gacggatggg gacacattgc agcagatggt tctattgcta
      781 atcttgaagg attatggtac gcccgttgca tcaaatcgat tcctttagct gtcaaggaag
      841 tgctgcctga aaaagtgaaa aaaatgtctg agtgggaact tttgaattta tctgttgaag
      901 aaattttgga aatgaccgaa agctttacgg acgaagaaat ggatgaggtc aaagcagctt
      961 cttcccgtag cggaaaaaat atccaaagat tagggaaatg gctggtccct caaacaaaac
     1021 actattcttg gatgaaggcg ttagatattt gcggagtcgg cttagatcag atggttgcta
     1081 tcccagttca agaggattat cgaatggata ttaatgcttt ggaaaaaaca attcgtgaat
     1141 tagctggaca aaaaatccca attctaggtg tggttgctgt cgtaggaacg acagaagaag
     1201 gtcaagtaga cagtgtagat aaaatcgtcc aattaagaga aaggttaaaa gatgaaggga
     1261 tctatttcta tttacatgtt gatgccgcat atggcggcta tgcgcgttca ctgtttctaa
     1321 acgaagcagg agaatttgtg ccgtatgctt ccttagctga attctttgaa gaacatcatg
     1381 ttttccacca ctgcgtaacg attgacaaag aggtatatga agggttcaga gctatttcag
     1441 aagcggattc tgtcacgata gatcctcata agatgggcta tgtcccttat gctgctggag
     1501 gaatcgtaat caaacataaa aatatgagaa atatcatttc ttatttcgcg ccatatgtgt
     1561 ttgaaaaatc agtaaaggct ccagatatgt taggagctta tattttggaa ggatcaaaag
     1621 ctggagcaac cgccgccgca gtctggacag cacatcgtgt attgccattg aatgtgaccg
     1681 gatatggtca attgattggg gcttcaatcg aggcagctca aagattcaga gaatttctcg
     1741 atcatctgac ttttactgtt aaaggaaaaa caatcgaagt ctatccattg aatcatcctg
     1801 attttaatat ggtcaattgg gtattcaaag aacaaggctg tacagatttg aacgctatca
     1861 atgaattaaa tgaaaaaatg ttcgatcggt cttcttatat ggatggcgat gtttatggtg
     1921 aacggtttat tacttctcat actacattta cacaagaaga ttacggtgat tctccgattc
     1981 gttttgttga aagaatgggt ctaacgaaag aagaatggaa aaaagaacag aaaatcactc
     2041 tgctacgtgc ggctatcatg actccttatt tgaatgatga tcgaattttc aatttttata
     2101 caaaaaaaat cgccaaagca atggaaaaga aactaaacga aatcatccaa tagaactgat
     2161 ttttgtgtga aagaataaca agagacatat ggagaacaga caattatttg cttcatcaaa
     2221 acaattgtat ttccccctgt ttttcatata ttcctcgcca agatattctt ttttataaaa
     2281 cgttctataa gttggatcgt tcgtttcatg tatcaaaccc agttcactaa gatcgttcaa
     2341 tggatgtttc ggtgatactg ggttttgtac tgaatcgatg aaaccttgtc atttctgttc
     2401 ttgaacaaaa ataaaagaaa acaaaaacat atgtcgctat tgaaagaagg aataaagatg
     2461 aaagatactg gttcatcaaa gcaaatcacg accatgacct ttatcggaat gacctgcgcc
     2521 cttgtagcaa gtgtacgaaa tattccagat gtagcagcaa cgagctggac gatgattttt
     2581 tacatgctgg ttgcagtatt attgtacgct tttccaatct cattgatctc tggtgaattt
     2641 gctggaatgt ttccccaaaa aggtggtcca gaactttggg tatcgaatgc tctaggaaaa
     2701 aaatgggggt tcgttgtctc atggctgtta tgggtccaaa tgtttcctgg gatggtgatg
     2761 gtcgcttcag cactcgctcc gttgtttggt aatattatcg ataatgtgcc gcttggtttg
     2821 aacagcaaat ttacattagt tgtcatttta gtcgtttatt ggatcatcac ttttttgaat
     2881 ttgaaatttg atatggcaaa aatcggcgga aaagtcggtg tttggttagg attgtacgtg
     2941 cctttgacga tgatgctctt attagggttt gctgcgtgga tcaaagttgg gatcgtacca
     3001 accggaacat taggcagttt ttcatgggac aaattaatac cagatactac gactgcatcc
     3061 tcatttgttt attttgcgcc gatcatgttt atttttactg gtatcgaaat gtcctctgtg
     3121 tatatcactc gattggaaaa tccagttaaa acgtatattc gcggagtatt tgccgcgtta
     3181 atttttatat ttttcgtcaa tattttgaat gctttagttg tagcaaatgt cgtaccaaaa
     3241 ggacaaatgg aattgaataa tatcgcgcaa tctatttcga tttattgcca aattcttggt
     3301 ttgccacatc ttattgtgaa tctgtttagt ttacttgtct tcattggtgt agcagtccaa
     3361 ttatctgctt gggcctcagg accagcaaag acagtgacag aaagtgctag aaaaggtgcg
     3421 tatccaccaa aattcaattt ttggaagacg aatcaatttg acgtatccaa atccgttatt
     3481 ttgactcagt ctgtcattat ctcaatcttt gcactttttt atttgctgat tcctggcgtc
     3541 aatcaagcat ttttgatgct cgtaaattca actactgtta tctactgtat cgtctatgtc
     3601 atcatgggga tcgcagtttt gagattgcgt tatacacatg caaagttgaa tcgaccattt
     3661 agaattggaa aaaaagagaa aaagagcaat cttggagttt ggactgtcgt tattgtttta
     3721 tttgcggcaa tcggattttc tgtcggactg acgatgaaag ctggaacatg gatcaatcta
     3781 attgcagtca cagccatcag tgtcattttg tttgtagtac cactctgtat tgaaaaaata
     3841 aagaaacctt cttgggaaca ggaagtcgcc atgctgcttg aaaaagaaaa aggtgaactt
     3901 gatggaaaaa ccggcaatga aacggactct aactaatcga aatatccagt taatcgcatt
     3961 aggtggcgca attggaacag gattattttt aggttcagca aaggcaattg catcagcagg
     4021 gccagctgtt attctcgttt atttgatatg cggtttggct atttatggtt tgatgcgggc
     4081 catgggtgat ttatttttag caaatagtac atttaactct atcagtgatt tcgttgacta
     4141 ttatttaggc aaaaagtggg cattttttgt caattggact tattggcttt gctggcttgg
     4201 agctggactt gcggaattaa cagcaatagg attgtatatc catttctggt ttgtacatgt
     4261 accagctttg ataagcggag gtagcgcact aatgatctta atggcagtca accttgttgc
     4321 agtcaagtgg tttggtgaat tggaatcttt tttctcaatc atcaagatct tggggatttt
     4381 cctttttatc ttattgggta taggattgag tgtctcagat tttcataccc agtctatcgg
     4441 aatcaaagaa aattttggac agatcgatga attactaccg tttggctttg ctggaatggt
     4501 agtctcattt cctatggttt tattcagttt tgctggaatt gaaatgatcg gcttaaccgt
     4561 tggcgagaca agtgatccgc aaaagaattt gaaaaaagca atcaactgtc tgccttggcg
     4621 tattctcttt ttctatatag gaacgatttt gtcgatttta tttgttgttc catggcattg
     4681 tttagattta aaagaaagtc ctttagtgac tatgctgcaa gtgatccatt gccaaggcgg
     4741 agcagcaatt gtcaatctaa tgattttggt tgcttctttg tcctcagcaa atagtgcagt
     4801 ctacagtact agcaggattt tatatcaaac agcaaaagaa aaaagtttgc cccattggtt
     4861 ttgtgcgctt tctaagaaag gcgtaccttt acacggtatt ttaattacag gaatgttatt
     4921 ttttgccggt ctttgtctac atttttttgt agctgatagt cgattgtttt ttcagctttt
     4981 ggcaggcatg acaacgagtt gttttctatt tgtgtggtct tcgatcttgt gtgcacatgt
     5041 tcaatttatt aaagaaaaga aacggaacac tacacaaaga tataaagatc ttgctttgtt
     5101 ggtgtttttt acttcaattg ctttcagctt gttattcgaa agcacgacac gctttattcc
     5161 ttttttattg gttatctggt ttgtattctt aaattttgta tataaaagaa taagcaatag
     5221 aaatatcagt taggcccaga tacattttgc aaaacgaatt atttcagacc cccctgaaat
     5281 aatcgatacg cctgaaaaaa cttccctgcg tataataaca gtatcaatta aacaggaggg
     5341 gataggatga actggtacag attgacggta gaagatgtat tgaaaaacta tcaaacatca
     5401 acagaaggct tgtcgcaaga agaacgacag aaacgcctga ctgatcaagg atataatgaa
     5461 ataaaagcaa ggcagcagac aaaacgatgg aaaaaaatag caaagcattt tactgatctg
     5521 ctgatgatcg tgttgatcat tgcctctttt ttgaaatttg cttctagtga atatattgaa
     5581 ggaagtatta ttttattcgt agtgatcgtc aatggactcg ttagttactg gcaagaaaga
     5641 aaagcggaag aatcattgaa tggtttgaag cagctgatgg gacaagaagc gatagtacta
     5701 tcaaacggga tgcaagaaaa aatccctgcg aaagaactgg taaaaggaga tgtcattgtc
     5761 ttacgtgccg gagatgtcgt tccagcagat atccgattaa tcgaagcaca tgatttatta
     5821 gtggaagaat cgattttgac gggagaatca gaagcatctg aaaaaagtca tgccgttttg
     5881 acagaagaag aatcgatcgg cgaccagaaa aatatgggtt tttcaggaac gcttgtacaa
     5941 tctggttctg cagtcggtgt cgttgtagaa acaggaaaca cgacagaaat cggtaaaatc
     6001 aatcaagcat tgcaatcggt cgaacaacaa acgacgcctc tgattaaaaa aatcaaccga
     6061 ttaaacaaac aaatttttca aggcattctt tgtttgagcc tattcttagt tatttttacg
     6121 acatttcgat atggtatgga ttggcatatc cttctttcgg ctacgattgc tttggtggtg
     6181 tcaatggtac ccgaaggatt gccagctgtt ttgaccatga ttctatccgt tggagtacat
     6241 gaaatggcaa aagaaaaggc gatcatcaaa ggacttcctt cggtagaaac attaggttct
     6301 atgacagtga tctgttcaga taagacagga acattgacaa aaaatgaaat gacagttgtg
     6361 gatgtagctg caaaagaaga agcctgcgtg ttgtccatta tgaaaaactg tcaggaatta
     6421 aaaacaaaag aagaacagaa aacagaaaat ttaagtggca atccgacaga agttgcgctg
     6481 attcgacaca cagaaacagc caatctacct ttgaaaaaaa tagaagcaaa gattccgttc
     6541 agttctgaat acaaatatat ggcaacgatg catgcggagg aaaaaggagc ggtgatttac
     6601 gtcaaaggtg caccagaagt attatttgct aaatcaactc tttcttctgc agaacaagaa
     6661 gcatggagtc agacagctgc agaatttgcg agaaaaggtc agcgtgtttt aggatttgct
     6721 tataaaaaag tagattcgaa acaggaactc acgcatgaga ctttaaccca attaactttt
     6781 gcagggatcg ccggattgat cgatccgcca aaagaaagtg cagtcaaagc tgtaaaggaa
     6841 tgccaacaag cagggatctc agtgaaaatg attacagggg atcacaaaga cacagcaaaa
     6901 gcgattgcgg aacaaattgg cttaaaacat actgccaaag ttttagaagg gatcgatttg
     6961 gatcttatgt ctgatgaaga gttgattcag caagtgccaa tagtcgatgt ctttgctcga
     7021 acgacaccag aacataaact gcgaattgtt aaagctttgc agaaaaatgg agaaatcgtc
     7081 ggaatgaccg gagacggcgt aaatgatgca cctgctttga aaagatccga tgtaggaatt
     7141 gctatgggaa tcaaaggtag tgaagtaagc aaacaggcag ctgatatggt tttaggagat
     7201 gataatttcc atacgattgc aaaagccgta aaagagggcc ggcggatttt ggataatttg
     7261 caaaaaacca tcaatttctt cttgccaact gcattagctc aaggattgat cttgatttgg
     7321 gcacttatgc tgaatcgccc gctacctttg tccccagtac agattttatg ggtcaatatg
     7381 gtaacgacaa tcactttatc ttatgcacta gggttcgagt cgccagatcc agaaatcatg
     7441 aagcgcccac caagagatcc aaagcaagga atcctatctg gttatcattt gtttcggatc
     7501 atctatgtct ccctactgat catggttcct gcctatttat tggctatgca gtttgaaggg
     7561 caaagcttac agcaaaccat tcttctgcaa aatatcgtct tagctcaagc tgtttacatg
     7621 atcaattgca gagagttgtc caagccgtca ttgaaccaag gtcttttgca aaacaaagcg
     7681 ctttttcttt cattaggtat ccttgcttta ttgcaaggga tggttatttt tctaccagtc
     7741 ggccagcagc tgatcggtac agtaagttta acgatgacac aacagttatt gattggagca
     7801 aatgtgattc ttctattctt agttgtagaa atagaaaaag ggatcatgaa caaactgttt
     7861 cgcaaaaaag aaaaaacagc tagagaatac agtcgctagc cgttactata aaaaagtttt
     7921 gaaagtgagt tcagtcaaac gattgagctc atttttcgtt ggaatagaag aaatgtatac
     7981 aatagggata tctagagaat ctagagggaa attggaaatc aagatatctg ttttttcaaa
     8041 agaaatagtg gaaagatcgg ttagttcgat aggtaagaag cagacacgtt ttcctaagta
     8101 atcttttaat tcctgctgca agaacgcctt ccatgaaggt tctccttgga aaataaaata
     8161 gacattgaac aaattcggtt tgatagaaaa aatcgtttgt tggatcagta gagacaagag
     8221 ttggagaaag taatttggtt cattgatttc tatttctttt tttagatcgt tcagtaaatt
     8281 ctctttctgt tgatacaagt gtgcatattg ctgatcatat gtttctaaaa aacgatggta
     8341 ttgacggtcc aatgcttcaa tcaatatcgg cgattcatga tattttaata tgaggagagc
     8401 caagttatca gtcagagatg ttttttttag ttgatcaaga gacagctctg aagctagttc
     8461 ctctacaaac gtttcgagga cctctaaaaa tggagaatga tgcaggcagc gagcggtttc
     8521 ttgtccatag ttattgttgt aattccaact ttccagaaag cagaaagaag caaaagaaag
     8581 ctcagtgtct ttaagataaa taccttccgt taaatacaat tttttcaatg cagagtcata
     8641 cagggaggag agtgtgctgt tggtatggat agaagaatct aatcggcaat tcgcttttat
     8701 acggatcgta ttgataaaga accaacatgt tccgaaaatc tcttctgttt caaccgctag
     8761 ttcagtttga ctgaggtttc ttaaaaattg aaaataatgg gattcatgga tcgaataatc
     8821 ctcgaaaaaa tagttgttat gagtgaaagg aatcagcaat cgatgataaa aagtacgaat
     8881 cgcacgctct tttccaagaa tacaagctgt atatttcgtc aaatcgatct gcaagtcgta
     8941 acttgctaaa cgctgattga ttttagtcac atgtcttttc aatgtcccat aagaaagccc
     9001 atggtcggct agaaaatcag ggacagcaac tttttttgta aaaagcagat ttttcagcag
     9061 ttcgacactg actgattgtt tgaaaaaaat ctcccaaagg tcatttgtta ataaattttg
     9121 ctggttttgc aaactgattc ctgcttgatc gctgatgatg tcccagcctt cgggcagctg
     9181 agagcgaata gattgaaggt cagaaaaaat cgttcgttcg gttgtcccta tgatttcagc
     9241 taattcattt acggttatct ttgtatgatt cagcaattgt tctactaaag aaacatgacg
     9301 tcgaatatct ttttcggtaa tgattttttt cattagtgaa tacatcaatc agactccctt
     9361 ctattctaag aatacggaga aaataaacgg tggtcaagtt tcaactgatt tttcaatcat
     9421 tgtttatttt tctggagcat ttactgatga taagaatttc atcatgaact tgaaaaatga
     9481 taccactaaa atcaaaaaat aggtgggcaa acaataataa acattttaat agtagatggt
     9541 agcaagacca acacaggcgg attgaacatt taagagttga taaaaaagaa aaatatcgaa
     9601 gtatttgatt agcaactact tgtattcatt tttctattct tacgtggtat ctttgaataa
     9661 agcgaatcaa taagaaagag atggaagcag atgctaacga aaaaagaact aatcgatcac
     9721 atcaatgaaa catacaattt gtctccagaa tatattttta aaaaatttcc tgattattgc
     9781 gtattcagac atcaaaaaag caaaaaatgg tttggggtcc tgatgtctgt tccagaagaa
     9841 aaaatctatg atgatgcaag tgaccatagg atcatcaata ttcttgattt gaaggtcgat
     9901 cctgaacttg gcggaatttt gcgaggaaaa gaggggtatt ttccagccta tcacatgaat
     9961 aaagaacatt ggatctctat tgatctacgt aaaatagaaa agctagatga actagcagat
    10021 ttgattgatg gaagttttaa atcaacgcaa tgaatgtcta ggacaataga gaagaggttg
    10081 tgacagaaat gtt
//
LOCUS       DACEOE010000091         9895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEOE010000091 DACEOE010000000
VERSION     DACEOE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.093"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(224..1072)
                     /locus_tag="IW774_001819"
     CDS             complement(224..1072)
                     /locus_tag="IW774_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1154165.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1236..2138
                     /gene="gnd"
                     /locus_tag="IW774_001820"
     CDS             1236..2138
                     /gene="gnd"
                     /locus_tag="IW774_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1154166.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2140..3141
                     /locus_tag="IW774_001821"
     CDS             2140..3141
                     /locus_tag="IW774_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1154167.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3138..3422
                     /locus_tag="IW774_001822"
     CDS             3138..3422
                     /locus_tag="IW774_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1154168.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3455..4252
                     /locus_tag="IW774_001823"
     CDS             3455..4252
                     /locus_tag="IW774_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1154169.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4266..5099
                     /locus_tag="IW774_001824"
     CDS             4266..5099
                     /locus_tag="IW774_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1154170.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5117..5974
                     /locus_tag="IW774_001825"
     CDS             5117..5974
                     /locus_tag="IW774_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1154171.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5996..6505
                     /locus_tag="IW774_001826"
     CDS             5996..6505
                     /locus_tag="IW774_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1154172.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6542..6943
                     /locus_tag="IW774_001827"
     CDS             6542..6943
                     /locus_tag="IW774_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154173.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7055..7408
                     /locus_tag="IW774_001828"
     CDS             7055..7408
                     /locus_tag="IW774_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154174.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7442..7885
                     /locus_tag="IW774_001829"
     CDS             7442..7885
                     /locus_tag="IW774_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1154175.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7902..8987
                     /gene="uxuA"
                     /locus_tag="IW774_001830"
     CDS             7902..8987
                     /gene="uxuA"
                     /locus_tag="IW774_001830"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1154176.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8980..9693
                     /locus_tag="IW774_001831"
     CDS             8980..9693
                     /locus_tag="IW774_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1154177.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3290 a         1567 c         2184 g         2854 t
ORIGIN      
        1 gaaaagataa attttttact actgtatact aactttgtta tcgcatctaa caagtaactc
       61 gaataatacc aatacaacct tctccaaaga tgcgatgaca tacgttctaa cccaccgact
      121 attgatgttc tgtaccaccc ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg
      181 gctttttttg ttttatggat ttttcgcttt ttctgttatg gtgctattct ttgatttcag
      241 aaatagtatg tcgaacttta gcgatagaac gacgtgattc tttttcgttg taaaacatca
      301 aaatcacata tagagagtcc aaaatgctta gttgagaaat acgggaagct aaagcttcag
      361 atcggaattc tgtttcttca gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg
      421 aagaattagc ttggctagta ataacgattg tttttgctcc agtttttctt acggtttcag
      481 caatatggat tgtttctttt gattgccctg tgtgagaaat caaaacagca cagtcttttt
      541 ccgtcaataa agaagcttcc atcaattgaa tatggtaatc cgtgctgtga gaagttgcga
      601 taggggaacg caaaaattta tgataagcgt cagttgctac aatctctgaa ccgccaacac
      661 cgaaaaaata gacacgttta gattcattga taatagtagc cgcttttttt agatcctttt
      721 gttgtaacaa cttttttgtg tcatttaaag ttgaaatact agactcgaac accttttgcg
      781 ccatagtgag ctcagtatct gttttctgaa tattttcgtg gatagaaata gcagatagat
      841 cattttcttg gatcaacatg gccattttaa aatctttaaa tccattataa ccaagttttt
      901 ttgtaaattg aaaaaaagta gagtcagcaa tgcccagttc acttgctaaa ttgctaatag
      961 gactatgact tactttgctt ggattttcta aaatataatc ggcgatagcc tgttcttttg
     1021 ggctgaagtc gttgtacatt gttttgattt gtagtcttat ggagacgctc atgagaattc
     1081 ctcattttca taataagatt atcatttttt ccgttctcat taatgttaaa gatacaagga
     1141 gagtaggaaa aaatcaaaca aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt
     1201 tgtataatga gggtataaga aatggaggcg ctatcatgga aattggtgta attggtttgg
     1261 gaaaaatggg attgaattta gctttaaact taaaagagca ccatcacaaa gtacaaggac
     1321 tcgacatttc aagtacagct gttagtcagg caagagaaaa tgacattcct gcttatcaag
     1381 agatctctga atttctagct acttttgagc agaaaaaaat catctggtta atgcttcctg
     1441 ctggagagat aaccgaaaac atgttaagaa aactttttcc aaaactcgga ccaggagaca
     1501 ttattattga aggcggaaac tcttattata aagattcaat ccgtcgagca gaagagtttc
     1561 agaaaaatga aatcggttac ttggattgtg ggacttctgg aggaatcgaa ggagcacgac
     1621 atcaagcctg cttaatgatt ggaggagata aagaaacttt tctatccgtg gaacaattat
     1681 tcaaagatgt agcaattgaa agaggatatc tgtatgctgg tgcatccgga agcggacact
     1741 tcttgaaaat gatccataat ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat
     1801 tccaacttgt acaaaaaagt gagtatgctt ttgacttgga acaagtcgca cgtgtttgga
     1861 atcatggttc ggtgattcgc ggctggctga tggaaatcat ggaagatcag ttaaacgaac
     1921 atcccaattt aagtaattat cggggaatcg tttctgcatc aggtgaagca aaatggacgg
     1981 ttgaaacagc gctagaaatg gctgttccag ttccagtaat tgcaactagt ttgttcatga
     2041 ggaatctttc acaggaagaa gatagttttt cagcaaaagt agtttcagca ctaagaaatg
     2101 ggtttggcgg acatgaaata gttgaaaagg gtgagtaaaa tgccagatta tcatatagca
     2161 atcgttaatt ccagcagttt cggaagagtc tttccacacc acttggaaag attaaagtca
     2221 gttggagacg ttcatttctt tactgttgat caagagattc caggaaaaga actagcggag
     2281 ttacttaaag gattcaatat cattattgca agtgttacgc cattttttcg acgagatttt
     2341 tttgaaaaca aagatgaact gctattgatt tcccgacatg gtattggtta caacaatatt
     2401 gatttaaaag cagcaaaaga acaccagaca atcgtatcta tcatacctgc tttagttgaa
     2461 cgaaatgctg tagcagaaaa taatataact aatttgttgt ccatgcttcg acgcacgaca
     2521 caagcacaag caagagtcaa ggacgatcga tgggaagatc gggcggagtt cgtcggtagg
     2581 acgctgttta acaaaacagt tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc
     2641 gaaattttgc gtaacggttt tcgctgtgat gttctagctt atgatcctta caaatcagat
     2701 ttatacatcg aaagctttgg tgcaaaaaaa gtagaacttc aagaattact ggaacaatca
     2761 gatatcattt gtttatgtgc gaatttgaca gaagagaact atcacatgat ttctgataaa
     2821 gaagtcgatc agatgaaaga aaatgtttat ctatctaata gtgcaagagg cgctctagtt
     2881 gatgaagaag cgatgattaa tggtctgaaa agcggaaaga tcgcaggata tgccacagat
     2941 gttttagaag aagaaccggg acgtcaagat catccgtatt tagcgtttga acaagtagtg
     3001 attacgccac atatatcggc ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt
     3061 gtcagagatg tcgaacagat cagtcagcac cttcttcctg aacgttctgt ccagacaaag
     3121 agcaactata taaaaggatg atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc
     3181 ctcaatttta tttatatgaa gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat
     3241 acggcgcgaa aagagtactg gtcacccacg gaacggtatc atgggaaaag gcgctaccta
     3301 aattagtatt tttgaatgac gaaacgattc aattttttta tcatcgttat tctggagaat
     3361 gtagttatgc tgaagccaga cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt
     3421 agttggcgtt ggcggaggaa aactgaccga tctagtgggt tatagtgcca atcttgcaag
     3481 aatcgatttc ggagtcatcc ctactttagc tagtaattgt gctccatgga cacctcttgc
     3541 tgtcatgtat aaggagaatg gacaagcaga agggaaaaca gaacactttt acagacaagc
     3601 tgcttttttt ataacagatc cccaattagt gattgattcc cctgttccat atttcattgc
     3661 cgggttagca gatacactag caaaatggta tgaatctgag ctcatcttag aacaagattt
     3721 tttgcaagat gaatcatttt tgaagctagc acaagatact gcgaagatct gcaaagaaga
     3781 gattttgaat cattctgcta aagcaatcca agatatggca caaagaaaac tgacatcgga
     3841 gttcaaacag ctttcagaga ttatctttgc tgttgctggt ttagttggag gattaggaga
     3901 taaatacgca agaaatgtgg cagcacatgc tatgcatgac gctatcagta aatatattcc
     3961 ggaaagccat cgttttctac atggagaaaa agtagcgtat gggattttct atcaactggc
     4021 actagaaggt cgctgggaaa aaatagacga gttatatcct ttgtatcggg aattagaact
     4081 gccggtttct ctagaacaaa tgggattatt tccgaaagat aaacagataa tagataaaat
     4141 ggctgaattt attgattcga aagagaaagt gcatttgatt cctatagagg taacacagaa
     4201 atcactgata cagtcaatga tggaactgga aggttatatc agaaagatct gaaggaggag
     4261 gacacatgga aacattaagt gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg
     4321 ttatgtcccg ctggttaggt ggaggagcca cgttgacctt acgtttttct cctttgatga
     4381 ctggattagt cgtcgggttg atcatggggg aagttgcaca agcgatgatt gttacagccg
     4441 ctttgcaaat gatttatatg ggggtttttt ctccaggagg atcaatgcca gctgaaccat
     4501 ctattgcagc agctatcgca gtaccagtcg cattattagg ggatctgtcg ccagaagcag
     4561 cgattgccgt tgcggtgcca gtcggattgc tcggaagtta cttgtatcag tttcgttttt
     4621 tcatcaatac ttttttaggt aaatatacag acaaagctgt agaagatttg aatacaaaag
     4681 ctatggcccg atcaattatt ctttatccaa cggttgcctc atttttatta ttcgttccgc
     4741 ttgttttcat tgctttatat ttaggggcgc cattgatcgc tgatgtaatc aaagctttag
     4801 agggaacagt cgttatccac attcttgaag tagtaggtgg agggttagcg gcaatcggta
     4861 tcgcgacaac tgtatacgtt atcggtcgaa aagattatct ggtctttttc ttccttgctt
     4921 attttatgag tgttgttttg aaatctttag atattacgat ggttacttat gccatttttg
     4981 ggattattat cgctttgatc tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca
     5041 caagcaataa caattcagca tcttttgatg acgaagatga ctatgatgac ggattttaat
     5101 ttaggaggaa tgggaaatgg ctgaaataat ggataatcaa acaggtgctc ctgaagaaat
     5161 cacgaaaaaa gatgtaacaa aagcctatct gagatggcat tttgcaaacg aaattcctca
     5221 ctcttttgaa cgttacttgg cgccatcact actatacgga atgatgccaa tcttgaaaaa
     5281 actttataaa gatgaagact ctttaaaagc agcatataaa cggcagttac tgttcttcaa
     5341 cactcaatta agctggggtg gcggtgtgat tactggccta atgtcttcta tggaacagca
     5401 aagagcagaa gaagagtatg aagaaaaaga gatcttgatg caggatgact tgatgtataa
     5461 cacgaaagcg ggattgatgg gggcactagc aggaatcgga gatgcaatcg attcaggaac
     5521 cgtccaatat atttttatcg caatcgcggt accttgggca caacagggaa gtcctttagg
     5581 cgcacttttt ccttttatct gctttgggtt gtatcaggtt ttactagggg tatttttcgc
     5641 aagacaagct ttcactatgg gccgtaatgc tacaggccta aagcaaaaca ctggtgttca
     5701 gacagcgatt gaaatgctat cagttttagg gttgtttatg atgggggtgc ttgcgggaaa
     5761 ctatgtaaaa gtagaatctt cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca
     5821 ggaaattctt gatcaaatcg tgccagggat attaccttta gctgtcgtaa tgggtgtgta
     5881 cttgttctat acgaaaaaag gattgaaagt cacacaagca ttattatggc tgactggaat
     5941 attaattgtg ctagcaggaa tcggtatttt ataaaaagaa gggaacgtga ataagatggg
     6001 tgtagtcaat ttagcgcgtg tggatgagcg tttgatccat gggcaagtaa tggtaacgtt
     6061 atctcaaaaa agtggagtga attccatttt tgttgtagac gaagttgtag caaaagataa
     6121 atttatgaga gatctttaca aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat
     6181 tacaccagag aaagctaaat tctattggga tgaatatcaa tttaaagaat acaattgtat
     6241 tttgatcgca aaaacagtcc aaacgattta cgacttagta aaacacggaa tccccatgga
     6301 agaattgaat attggaggga ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa
     6361 atctgtttat ttaaataaat cagatgctga aaaattgaaa gaaatgaatg aacaatatgg
     6421 cgtaaagaat atttattttc aagccactcc atctgcacct aaaacaggat taaaagatgt
     6481 attgaacaag tttgatttgt cataaacaaa tgcttagaaa aaagataaag agggcgaaaa
     6541 aatgaagaag aacgagctgt ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa
     6601 gcgccgtaca aaaaagagta aacagcgttt tctttcagca ttagtatcag atatttacag
     6661 tatgagaaca gatgtgacag tcattgctta cgatacgcta gcctaccgat caaaaaatat
     6721 ttatgttgga gatatcgaaa aagcagagaa agttatctgt acttattacg atacacccgt
     6781 tcatgcatta ggaagttatt tcatgtttga ttggaaagac caaaggaaaa aaacaatcta
     6841 ctctatatta ttgtccttca ttttgttgtt cagtttaggt tggtggggaa tgatgatata
     6901 taacaaaaat cctcatcacg tattcgatct gctatccgta taaacaggta tcactgtact
     6961 agcatttggg agctactttt tcttattggg aaaagcagct cgcggatggc ctagcagaca
     7021 aacttttatt cgaaatactt catccatatt aacgatgcta gaaatgatcc gtacgattga
     7081 tgatccgagt gtagcgtatg cgtttgttga tgaaggctgt tatggagaaa agggcttaga
     7141 cagcgtgaga tcgggtatga aaaaagaggc tattcttttt tatctagact ctgttggggc
     7201 agatacaccg ctacagtttt cgggaaatta tttttcaaat aaagaacagt ggctgaaaca
     7261 agttgataag ttgaaagaaa aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc
     7321 gcagttcttc tatttgacca aaacagattt acgagggaaa acatttaatt ggcaaaatgc
     7381 caatcagatt attgctcttt ttcgataagt aaaaagtgca aaacaaggag tgagaagact
     7441 aatgttggaa atcatgatcg ttacacatgg taagttgagc gaaggactga aagatgcggc
     7501 ggaagtaatt gtcgggacat cgagccatat tgaaacaatg tgcttagagt ctggggagga
     7561 catccaaaca ttcggagaga aggtcaaaga aaaattaact atgatcaaca cagcagaacc
     7621 catgctagta tttgttgatc taattagcgc aagtccgtac aatcaagcgc tcttggcagt
     7681 caatgaactg actgaagaaa agaaagcagc cacttatgtc atagggggcg tgaatctgcc
     7741 aatgttgtta gaagcaatca atcatcagat gcttcacaca ccgatagaag aagcggtgga
     7801 acaaattgtc aaacaaggaa aagataattt aactgtttgg catgcttcag cggaagaaaa
     7861 aaaattagat gaggacgatt tttagaagga aggctgaaaa gatgaagtgg ggatttagat
     7921 ggtatggtga aaaaggggat tcgatcccat taaaatatgt tcgccaaata ccgggaataa
     7981 atgggataat tggaactttg ctgactaaaa tgcctggaga tgtttgggag atttcagaaa
     8041 ttgaggagtt gaaagcatct gttgagagcc aaggactgga actgcttgga attgaaagtg
     8101 tagcgatcca cgatgcaatc aaggcaggga cagatgaacg agattactat attgataact
     8161 atatacgaac aatcaaaaac ttgtctcgat gtggggttcg attgatttgt tatagcttca
     8221 aaccaatttt tggatgggcg aaaactgatt tgtttaatga aaacaacgat ggaagctatt
     8281 ctcttttata cgatcagaaa atcgtcgatc agatggagcc aggagaaatg tatcaattga
     8341 tacacagcca gtcaaaggga ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat
     8401 tcgctcagct ggaaaaactg tatgaaggaa tgaccgaaga agaattattt gaaaatctga
     8461 cttatttttt agaaaaagtg attccagtgt gcgaaaaagc agatgtaaaa atggctatcc
     8521 atccagatga tccgccatgg gaactttttg gttttcctag agtgacgaaa aatcttggcg
     8581 acttgaagaa aattctaaat gcagtagatt ctccatataa tggactaacg ctgtgcacag
     8641 gatctttagg ggcaaatccg gaaaacgatt tagttgaaat catccatgag gtaggttcgc
     8701 gcatccattt tgtccacttc agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa
     8761 cggcacatcc ctctacagaa ggctcccttg atatgtatca tattatgaaa gcgttggtgc
     8821 aagaagggtt cgacggtcct atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg
     8881 ctatgcctgg atatggcctg tatgatcgtg caatgggact cacttacatg caaggtctat
     8941 atgaggcgat aagtaaggaa aaaccactgg gagagcatta tgaataaaat caagctcttc
     9001 atctttgata tggatggcct tttattagaa actggacgtt tagcttatcg tgcttatgta
     9061 aaatcagcac aaaagtatga ttatgaaatg cgtaaggaag tctattattt actaactggc
     9121 caaacagaaa tggcgataag agaacaaatg ggcattttgt acggagaaga tgttccatat
     9181 atcaaatggc gagaagcaat caatcaatat aaagaaaaaa tcgtgaaaga agataagaga
     9241 gtatatacga aaaaaggagc agaagaaatt ttatcttttg caaaagaaag aggaatccat
     9301 acaatcgtgg cttcgtctaa tacacgggta aaagtagaaa tgtatttaaa aatggaaaat
     9361 ctatatgcct tgtttgatca aatcatttca ggggatgatg taaaaaaagg aaagccggaa
     9421 ccagagatct ttttgaaagc ttgttcaaaa atgaatattc ctccaagtga agcacttgta
     9481 tttgaagact cgattgcagg gatcgaagca gcaaggcgag cgggcatttt gtcttttctc
     9541 atcaaagatc atttgatagg gttgcccgat cataatggga agcataagtt gaaagtagat
     9601 gtaagtttat atgaatccaa taaagcagac tatgaatttt atgacctgca tcaagcaaaa
     9661 cgatttttag aaagtaaagg gttgtatctc taataaagag gactattttc tagtcctctt
     9721 tatttattgt cctgcacgac tgagggaaca gatgaaaaag aagtaatttt ttattttgaa
     9781 gtatctacct tatttgtagt gaacaattta gaacaaacac aaaacgcatc tactgttatc
     9841 taccagtaga tgcgtttaaa gagaagaacg agaatcgtcc atatgagggt ttcca
//
LOCUS       DACEOE010000092         9859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEOE010000092 DACEOE010000000
VERSION     DACEOE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.094"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..339)
                     /locus_tag="IW774_001832"
     CDS             complement(<1..339)
                     /locus_tag="IW774_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1154178.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSEL"
     gene            452..1372
                     /locus_tag="IW774_001833"
     CDS             452..1372
                     /locus_tag="IW774_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1154179.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            1445..2380
                     /gene="murB"
                     /locus_tag="IW774_001834"
     CDS             1445..2380
                     /gene="murB"
                     /locus_tag="IW774_001834"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1154180.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            2678..4156
                     /locus_tag="IW774_001835"
     CDS             2678..4156
                     /locus_tag="IW774_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1154181.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4433..6493
                     /locus_tag="IW774_001836"
     CDS             4433..6493
                     /locus_tag="IW774_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154182.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            6576..7145
                     /locus_tag="IW774_001837"
     CDS             6576..7145
                     /locus_tag="IW774_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1154183.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7320..8126)
                     /locus_tag="IW774_001838"
     CDS             complement(7320..8126)
                     /locus_tag="IW774_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154184.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8264..9778
                     /locus_tag="IW774_001839"
     CDS             8264..9778
                     /locus_tag="IW774_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1154185.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3533 a         1619 c         1964 g         2743 t
ORIGIN      
        1 taattcgctt tgagaatttg gcgtgtaaca ggttacaaga aagaaatcgg aatattccaa
       61 agtaatcaga cggccttctg tatcatggat atcgataccc attccgtaag tggcattcaa
      121 tgctggtttt ttagcaaaaa tcgctgtgcc ggaatagcct tttcgttccg cgtaattcca
      181 atattgataa taacctggaa gatctaaatc aatttgtcct gcttggagtt tcgtttcctg
      241 cagacagaaa aaatcggcgt ctaactcatg aaaaacttct aaaaaatttt tatttacgat
      301 ggcacgaagc ccgtttacgt tccaagagat aaatttcaat aaaagcacac tccttcgaat
      361 taatttcctt acctttgcga tactattgta cctcatttta gtaaaaaatg gtgtaagatg
      421 aaagtaacta aatgatccag gaggaaagta gatgaagatt ggtgtaatcg gtgtagggaa
      481 tatcgcagaa aaagcatatt taccaactta tgcgaaaaaa caaggaacac ttgattttta
      541 ttttgcaaca agaaataaag aaacaaaaga aagaattcaa aaagagtacg ggttccaaca
      601 tctatatgaa acgatcgatg agcttttaga agaaaaaatc gaagcatgta tgatccacgc
      661 agcaacaaaa gtacattatg aattagccaa aaaatgtcta gaaaatggta tccatgttta
      721 tattgacaaa ccactgagtg tccatctaaa tgaagttcgt gaattgcaag aattagctga
      781 taagaaccaa ataattttga tgatcggttt caaccggcgc tttgctccta tggtagaaga
      841 attgaaacgt atcccagata agcggttgat ccacctgcaa aaaaatcgaa tagcggcaaa
      901 agaaacgaca gagttcgtcc tttatgattt gtttttacat cttattgata cagctgtgta
      961 tttattagat gaacccattt tgagaacaaa ataccagata agagaaacaa aggaattttt
     1021 ggaatatgcc attttacaat tagaaacaca aaatcaaacg gctattttaa cgatggattt
     1081 agcaagtggc gctaatatag aaaaatatca agtaacaagt aaaaatggta cttatgaagt
     1141 aaacgatctg acccagcttg tcattcaaga tgctgatgga aaaaaagtga aagaatttgg
     1201 cgactggaca aacacgctag tcaaacgcgg gtttgagcca atggtagagg ccttcttttc
     1261 tcagatccgt ttaggtaaat cggaaacaga attgcttaaa caaaaagaag ttgtaagaag
     1321 tcatgaatta tgtgaggaag tgcttaaaga tcactaccgt catcaacttt gaacaagact
     1381 ggcaagattg tattaagcgt gatagaataa tgaggacgtt tgagaaaaga aaggaaaatc
     1441 ctcagtgaat aaagaagaaa tagttgaaag cttagccacg attactatat tgtttgatga
     1501 accattgatg aactatacgt ttacgaaaac aggtggacca gctgatgtcc ttgcattccc
     1561 gaaaaaacaa gaagaagtaa agcaaatcat cgattattgc cgaatccatg atataccttg
     1621 gatggtatta ggaaatgcaa gcaatctgat cgttcaagat ggcgggattc gcggtgtagt
     1681 catcatgctg actgaaatga aacagattca tgtcaaagga acgatggtca ttgcggaagc
     1741 aggtgcctca ctgattgata cgacttatgc agctttggca gaatcattga ctggatttga
     1801 atttgcttgc ggcatcccgg gaagtgtcgg tggagcggtt tatatgaatg caggtgcgta
     1861 tggcggagag atcaaagatg tttttgctga agtggatctt ttgcttgaag atggaacatt
     1921 gaaaactttg acaaaagacg aaatggtatt ttcttatcgt cacagtaaag tccaagaatt
     1981 acgtgctatc gtattggaag ctcgattcag tttgcaaaca ggggattatg aagcaatcaa
     2041 agcaagaatg gatgagttga ccgaattgcg tcaatcaaaa cagccattag aatacccttc
     2101 atgcggaagt gtctttaagc gcccagtagg acactataca ggtcagctga tccaacaagc
     2161 aggattgcaa ggcctgaaat ggggcggtgc acaagtctct gaaaaacatg ctggattcat
     2221 cgtcaatatc gatcatgcga cagcgacaga ttatattgaa ttgattgcgc acatccaaga
     2281 agtaatcaaa gaaaaatttg atgtgtcttt agaaacagaa gtgcggatca ttggagaaaa
     2341 acaagcaata tcagaaacaa gcagaagttt agccaattaa taagtaatgg aacttaaaat
     2401 aagtaaaaga gcaggacggt ggccgtcctg ctcttttttg tggttgttta cgcaaatggc
     2461 gcaaatcttg tacattttaa cacaataaaa caaaaaatat agaatatttc ctcataaatt
     2521 tttccatttt ttgcaatatt ctcaaaaaaa agataaaaaa tgagaataac tgatgaaaaa
     2581 acaagcataa tccatcataa aatcagtata ggtgaaaaga tgaactttca tctgcatatc
     2641 aagtaattgg tatgaggcat tttttgaggt gtaggatatg aagttggatt accttataga
     2701 agaggaagat agagaaaaac tgctgctagc tagatattta gaatttgaga aaaagaatta
     2761 tgtagtgctg cctaagttat atgaaacatt aggtttgtct aaatttaaaa caagaaatta
     2821 tatcatagaa ttaaataaag aatttactga attcaaaaca aagccagaga tccaaatttc
     2881 agaagaagca gagatccatg tcgaacgtct gaatttgacg gatatcaaaa atttaagaag
     2941 aatctacttt caacgatcaa aaattgctcg actgttaacg gagatcgtgg attcaaaagt
     3001 aacgatcgaa aaatttgcag agaatcattt tttaagcata tcaagagcct atgtaaaaag
     3061 aagagaatta gtctcttttc ttgaacagca tcaaataaag ttaaagaaaa acatattagt
     3121 tggggaagaa ataaacatac ggaacgtatt atttggggta tatttcgggg tctttaatgg
     3181 tttttctatg ccatttagag aaagcgtcat gaatgaagca aagagtatcg tgcaatattt
     3241 tgaatatctg ttcaatttaa aactgtctgc aacaaaacga atcaaattca atttgctaac
     3301 cactattagt ttgttgaggg cctatgggaa tcaaccaatc ggaaaacgtt tcttttcaga
     3361 tgaatacttt aagagctcag aaggaatgaa gatcatcaag gatttatcac atatttcttt
     3421 tcttcatatc cctaaagagc agatcattga tgagatttct tatatcatgt tgtttttgat
     3481 cacggaagaa gaatttgaat tagatggcgc atttggactt gcggaagctc attttcatga
     3541 tatccacatg attagtcaaa aaatgtcagt ggaattgatt gatcaattac cattttcgat
     3601 tgaaatctct gaagaagaaa gactatatat acgaaaagaa cttcgtatgg gtatgatgca
     3661 tgtcaatcga aaaaattgga tattcgactt tgagatgact agctttacgt ctaaaaagca
     3721 gctacagttt ttcttcgaga cgtatccaat attcagtaca gcaatccaaa aggtcataca
     3781 agagaataaa cgctatttct tgggaaaaga tgaacgtttc atgattcgct tattttatga
     3841 ttatctcttt ttattggtcg gtatttgtcc ggtatcatat tttgaagcac cgattcatgt
     3901 ctgtattgat ttttcgcaag gatctgctta taccgattac attatttctc aagtgaaagg
     3961 atttaaaaat tacaatattg tgattgagag tcgaatcact tctcgtacga atatttattt
     4021 atctgattgt ttacttgaaa aatttaataa agaacagata atttggaaaa atccaccgac
     4081 tccagatgac tgggaatatt ttggaaatac catcatcaga gaaaaaaata gacatttaac
     4141 tgaaaaaatc agctagtatt tatggatggc agctttgtct cacatattta ttagctgatt
     4201 tatgtttttt tcaaagaaaa ggtaactaga tataactaga tataactaga tatattactt
     4261 aacaaaatta aaaagtacat aagttattat tccgattttc ttgaaacaat aaaaaatcaa
     4321 aaaaaatact ctcctaatag tccatcttct tttgatttta tttatataat gaaatcatct
     4381 tatgaaaatt actacttcat gtttagacaa gagtagggag gactaagaat aaatgtgttc
     4441 aaaaagacgt tcaaaacaac taaataaaca cgcttgtcgt ttagataagg aacggtcgtg
     4501 ttctgaaaaa cactacgtta agtttatgat gcgcacgatg ctgatcagtt tatctgtagg
     4561 tgtctatatg catgtacccg ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc
     4621 tgtttctgag accactgatt cccctatcac tattactcat actttcactg atgcaaatgg
     4681 aaaacagcta cttcctgcat tgacttccta taataaagtc agttatcctc caacgataaa
     4741 ccactactgg ttaattccta acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc
     4801 gctttctatc ttttccgata tgactgatcc tacaaaaggt gatcagttaa aacagatcac
     4861 tgatattttg aatcatgctt tgacagatat aaaggacggt ggaagcgcac aagtttctta
     4921 tgtatatgaa aaagattatt cacagtttga aagaaagaaa atcgatgttc tggcagggac
     4981 aaaagtttcg atatctgatc tggtagaaag gctgaagaac gcagatggaa cacaaggaga
     5041 tatgtccaaa gtaatttcaa acaatgggaa tatcgtggat acgactaata caggaacatc
     5101 tactatttcc ttgtcctatt ttgatccaat tgctttaaaa acaatgactg catctgctgt
     5161 gatccatgtt cgtgctgatc aaacagctat caaaggaaaa gatcttcact taagaacagg
     5221 agagagttac gcccctaaag atcttgttga atatgtaaca aaaagcgatg gaacttcagg
     5281 aaataaagaa gaaatcaaaa tgaacgggac agtagatact acaactccgg gagaatatcc
     5341 cgttgtatta acttatatag actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt
     5401 ttatgtagaa gcatctgctg cttcatcgac tgtcatcact cattttatag atagtcaaac
     5461 aaataaagaa cttcaagcag aaacacatat gagtagtgta aatcgtattc ctgatttgcg
     5521 cctacctgct gaattttctg atcgagtgcc aaatatagat ttatcgactt atgaacaaaa
     5581 tgggaaagtc acttctattt ctgattggat gcaagcaaac ggattagaca gaaaaagtca
     5641 ctggtcggag atccttgaag tacttcactc agaactgatg aataatagga agcaatcagg
     5701 aggggaaatt acctttactt atgtttatat accagacaat cgttcattag aaggtcatga
     5761 tcttattgtt catccaaacg aaaaaataaa caaagaagag ttgatccaat cagcaacaaa
     5821 ttccttaggt aaacccgtac cgttaaagga tgtatcggtt acaatcaatg aagtaccatt
     5881 ggaggatggt tatttgacca aacaaacagg tgattttacg attatgtata gttatcatga
     5941 cccgtattca ctccaagaac tccatgctca gtcactatta catgttttac cattaaacaa
     6001 acagatagat caaaaagaac aaaaaagaca agaagaaagc ttgtcagaaa aaaaacaaca
     6061 aatggctcat gcagtttctc caacagggaa agtaaaacaa acaagaagcc tagagttagc
     6121 aagaatatct agaaaaatct cagaatctaa taaaacgata aggcaagaag ctcatttggc
     6181 cccacaagtt tctggattgg aaaatgaaat tttagagcct aaaataaaac aacaatcaga
     6241 aaaagaattt gttgctaaac agtcggaaga tcagaaggaa tcagatgacc agaaggaaac
     6301 gaagaaaaaa cgcttgtcaa ctattgattc caccgatgag catctgcagc cgcaagtacc
     6361 acttcctagt ccgggaacac ccgcacctgg tggaggtatt ccttcttctg ctttaggaga
     6421 tttcatgcga ggtatggcag gaacatgggt atacgctaat cgaaatgaat ttgacgcaga
     6481 tgactggttc tgatttatat ctctatctgt tatagtcaaa taagtagctg agtcatatgt
     6541 tatttggcat aatggagacg aggagggata gaaaaatgtt agataaaacg ttaccttacg
     6601 cagaaatctg gatgacaaga ccacttcaac aaaatgttcc aatcacgcct ttagcggaag
     6661 gttttgaatt tcgattttat caaaaagaag atgaatctgc ttgggcggag attgagactt
     6721 ccgttgggga atttcaaacg acagatgaag cacttgagta ttttcaacaa aactttgctc
     6781 cttatcctga agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag
     6841 tagcaacctg taccgcttgg cgaaaaaaat caagagagca agtgtatccg gtttttcatt
     6901 ggttagcagt gaaaccagat taccaaggaa aagggctcgc aaaagcattg actgccagag
     6961 tcttgcaaga atttcctacc ctccatacgg acggaccaat ctatcttcac acacagacat
     7021 ggagccatca ggcgattgct ctttataaaa aaatgggttt tacttttatt gctgaaaatc
     7081 tagacggcac acctaatcca gattatgaaa aagttatgca ggttttaagt aaattagata
     7141 aataacgaaa tggtgaaata agtcctcagt attgggataa aaaacgaaat agtcattcct
     7201 catatcttac ttttttgagt taatactatc ggataactaa tggcatgcca tacattcaaa
     7261 aaagaagggg cagtgacaaa agtattgtca ctgccccttc tcttgttttt tagcatgttt
     7321 tatcgtttga tttttttagg aataaaattc ttgatgaacg taaatttttg gtaatcgttt
     7381 cgactataga ggtaaccaaa tacactgaaa acaactgctc caataaaatt aacaaataaa
     7441 tccttcatag tatccatgat tcccacgtct aagtaaccat tttggatgat tgttttatct
     7501 gttttaccat ctgcattttg actttcaatg attgtccgag tgatatctgg aacatgatag
     7561 acagtgtttc cgtctttact taactccaca ctattgattt gctggatgat cgtatctttt
     7621 tgcatatcga attgcagtaa tttatctgct gaaaattcca caaactccca cataacacca
     7681 actgtcatcg aaaaacagaa ggaaacaact gctagtaata aaggagtcaa gttcatggtt
     7741 tttgtatttt tattgagcaa ataaacaagg gaaaagccga cacccccagc taaaaatccg
     7801 ctgaatgcat gtagaaaagt atcccacatt gaaaaatagc cataaaaatc acttaactct
     7861 cctaaaactg ttgaactaaa aataaaagat aacaaaatga tttctagcat atttggaatc
     7921 tgtatcttta atatcttttc cagaaaataa gggatttcaa ataaaattaa tgtcagtaat
     7981 aaaagcattg tatttcccca gtgttggttc cagacctgat ccactagcat aataacgacg
     8041 atgatacgta ataaaatata gattccgttt gtgattctac ctgcagtttt tgtactgttc
     8101 gtaaaaaagg ttttcaaatg attcataaaa taactccttc atgtatatcc ttaattatac
     8161 taagaagcgt gtgtttacca aagctatatg acttaaagaa aaacagagtt gatattgtga
     8221 aaaaaatcac ttaatatagt ataaggagaa aagggggata caaatgaaaa aattagttgc
     8281 tggtttatta acagccgttg cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa
     8341 tacagcgcag acaagttcta gtacgaagga atctgccgaa gtatcatcag gcgcttctga
     8401 acaagtatat acagatccaa aagaaatgaa agaatcttat gatgtcgtga ttattggagc
     8461 tggtggtgca gggatggcag cagctatcga agcaaaagat gctgggttga atccagtaat
     8521 tttggaaaag atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc
     8581 ttcagaaaca aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg ccttctatga
     8641 agaaacgcta aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat actttgtcga
     8701 tcactctgct gatgcggtcg attggctaga tcaaaacggt atcaaactgg acaacttaac
     8761 gatcactgga gggatgagtg aaaagaggac acatcgtccg tctgatggct cggctattgg
     8821 tggatactta gtggaggggt tattgaataa cgtccatgag cgggaaatcc ctatttttgt
     8881 caacgcagat gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca
     8941 tgttcaagga gaagaaccaa aagaggtcac cggaaaagca atcgtagtta ccacaggcgg
     9001 ttttggtgcc agcaaagaat ttattgaaaa atatcgtccc gatttagctg attatattac
     9061 gacaaatcaa gaaggatcaa ctggagatgg tatcaccatg attgaaaaat taggtggaca
     9121 aaccgttgat atggataaaa tacaaattca tccaactgtg cagcaggatg agggggtctt
     9181 gattggtgag gttgttcgcg gagaaggagc aatcttggtc gatcaagagg ggaaaagatt
     9241 tgtcaatgaa atggatacaa gggataaagt atctgcagca attacagcat tgcctgaaaa
     9301 atcagcttat ttgatttttg accaaggcgt gcgtgatcgt gccacagcta ttgaatttta
     9361 tgaccaaaaa ggatatgtca caactggaaa gacaatggaa gaattagcag aaaaaatcga
     9421 tttaccaaaa gaaacactag caaaaacagt tgatggatgg aatacggatg ttgcggagaa
     9481 aaaagatgct caattcaacc gtgccacagc catggaacat gacttatcgt ctccgaatta
     9541 ttatgcgatc aaaatcgcac caggaatcca ttacacgatg ggcggtgtga aaatcaatac
     9601 gaacactgaa gtattagata aagaaaacca gccaattagg gggttatatg ctgctggtga
     9661 agtcacaggt ggcttacatg gaaacaaccg gatcggtgga aattcagttg cggagatcat
     9721 tatttttggg cgacaagcgg gcatacaagc agctcgattt gcttcggaaa atgaatgaaa
     9781 tagaacgtaa ataaaaagag gttgtgaaat cagcgttttg agctaatacc gagggtcagc
     9841 tgattgaaca ctgtttagt
//
LOCUS       DACEOE010000093         9809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEOE010000093 DACEOE010000000
VERSION     DACEOE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.095"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(32..2152)
                     /locus_tag="IW774_001840"
     CDS             complement(32..2152)
                     /locus_tag="IW774_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1154186.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2382..3560
                     /locus_tag="IW774_001841"
     CDS             2382..3560
                     /locus_tag="IW774_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1154187.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3576..4334
                     /gene="aroD"
                     /locus_tag="IW774_001842"
     CDS             3576..4334
                     /gene="aroD"
                     /locus_tag="IW774_001842"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1154188.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4357..4842
                     /gene="ybaK"
                     /locus_tag="IW774_001843"
     CDS             4357..4842
                     /gene="ybaK"
                     /locus_tag="IW774_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1154189.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4913..6166)
                     /locus_tag="IW774_001844"
     CDS             complement(4913..6166)
                     /locus_tag="IW774_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1154190.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6283..7632
                     /gene="gdhA"
                     /locus_tag="IW774_001845"
     CDS             6283..7632
                     /gene="gdhA"
                     /locus_tag="IW774_001845"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1154191.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7744..9090
                     /locus_tag="IW774_001846"
     CDS             7744..9090
                     /locus_tag="IW774_001846"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1154192.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9398..9784)
                     /locus_tag="IW774_001847"
     CDS             complement(9398..9784)
                     /locus_tag="IW774_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1154193.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3126 a         1782 c         2010 g         2891 t
ORIGIN      
        1 ttttgtcgct gctcctttta aatttatttt atcattcgga agaacttgtg gtcgttccgt
       61 gaagttcttg gtacgtttgc gttttataaa gatcaacagt tgattgtccg ttgtgttcac
      121 taaagacact agttgacttg tcaccaagag atttttcagc ttcttttaat ttctctaagc
      181 cattcttgta gtcataatca ctggtatcaa ttgggtctaa tccactattc gtatagaacc
      241 gaagaagatc accattattg atttggtcag aaacagctaa ctgtttatca actttttctt
      301 tcagtgaatc aatttcattc tgtacttcct cagtaggttc agtaatagct tcaccagttt
      361 cgttactata gatcgtacca ccgtagtaag tatattttgg tgtaacaaaa tcaccatcac
      421 ggaaagaaac aagctgttca tgatctttcg agaatagatc ttgtcctaac tgaatataat
      481 tttttgtatc tacacctagt aagtgaagta atgtaggcaa ggcatcaact tgtccgccgt
      541 aagtgtgatt gattccgccc tgtgtttgtc cagggatatg gatcatataa gggacacgct
      601 gcatttgtgc attgtcgtag tttgtccaag tagaacttgt ttttccaact aattcagcta
      661 gatttttatt tcttgaattc gagacaccgt aatggtcacc gtaaaggacg atgactgaat
      721 tatcatacaa gccgctttct ttcaaataat tgaagaattc ttgtacggcg gtatctaaat
      781 aattggctgt cgcaaagtaa ccgttgatcg tttcatcatt tgtattggct aacgggaatc
      841 ctgcttcgtc attggtgaat tttgaataag gataatggtt agatacagca atgaattttg
      901 aataaaatgg ttgttgcaga cgttccaagt attgtacgga ttgttggaaa aatggtttat
      961 catgcaaccc gtattggaac gaattgtcat cattaacatc ataataagaa gaatcaaaga
     1021 aataatcata tcctaaattc ttgtatgtct cgtttcgatt ccagaatgta ccagcgttcc
     1081 catggaatac cgcactaatg taaccttgtg tttgacttaa aatatcaggt gcagcttgga
     1141 aagtattttt tccgcctaat tgtgtgaaca aggagccttg attcaagcca aacaatgaat
     1201 tttccatcag cgtttccgca tcgcttgttt ttcctgcttt tacttgatgg aaaaagttgt
     1261 caaaactgaa cgtgctgttg ctgtggtaaa gactattgat gaaaggtgta acttcatgct
     1321 ggagaccatt ctcatctgtc aatttataat cgatcaggaa ttgctggaag ctttctaaat
     1381 ggatataaat cacatttttt cctctagcga gcccgaacaa atcgtcattt ggtgctgcat
     1441 aatgttcttt tacataattt tctacttctt tcatatcatt gggacttgct tctgcccgca
     1501 cttggtttgt ctggtaagtt tgaacaccgt cataaaccat gaaagcattc aagccaagat
     1561 atttgaccaa ataatcacga gagaatgttc gagtcagcaa ttctggacga tcagcttccg
     1621 ccataaaaag atttcctgag aaaatcataa ctgctaatgt agagatcgca aaagccattc
     1681 ttgctcggat cgggcgaggg tccaacttga ttttttttgc aatcagcaga acagtcaaga
     1741 taataaaatc gatccaatag atcacatcat atgggcgaaa cagtcgaatg gcactttctc
     1801 ccagaccact cgctactttc ccagcaccta aaatcgtgtt gatcgtaatg aagtccgtga
     1861 attctcgata ataagtgaca ttcgaaaaaa caagcaatgt caaaagaaaa taaatgacca
     1921 tcatcgtgat ataagcaact ctagggcgtc tgatataaag agcaattcca agtaataaca
     1981 atgtagttgc aatagggttg ataaacaaga taaagtattg aatgccactt tcgattccga
     2041 gatggaaatc aactaaatag gcaaaaatcg ttttcaacca aagtaaaacg actagtaaag
     2101 aaaaaaatcc gagcctctta ttcaaaaagg caggtgtttt tatacatttc aagataatct
     2161 tcctttctgc acttcttaat cacagaagtg tcctgaaacc atgttacaca gctcacttat
     2221 tctcatttat tttactagta tgacaatata gtgtcaattg aaaccagaag tttatgctgt
     2281 ttcattaagg ttttctttaa gcttcatgaa aaaatgagca gtaagacaag tcctatgttt
     2341 tttgttatac ttgggaagag aaagctaaag caggtgaagg aatgaaagta aaagtaaaaa
     2401 aaagagcaag caatcgattg aaaaaaggat atccactgat ccaaaaagaa gaccttgctc
     2461 atccccaaaa agaaaccacg caatgggtgg aattagttga ttcgcaaaac caatttatcg
     2521 caaaaggata tctaggaaag cagaataagg gaatcggttg ggtattgagc cagcagaaca
     2581 tcccatttga tcaatctttt tttgaagaat tttttgtgaa gtcaaaagaa aaaagaactt
     2641 tctttttttc agatgattct actaccgctt ttcgtatatt caatggagaa ggagacggga
     2701 tcggcggtct gattgttgat tattatgcag gttatgctgt tttttcttgg tataacgaaa
     2761 cgttatatgc acatcgtgaa ttattgattc aagcttttcg tgaaatctac ccggaaatca
     2821 taggaggcta tgagaaaatt cgatttgaat cagtaaatct gcctgagagc cagttcattt
     2881 acggaaaaga ggcaccggaa cccttgatcg taaaagaaaa cggagtaaaa tttgccgttt
     2941 atttaaatga aggattaatg actgggatct ttttagacca aaaagaagta cggggatcat
     3001 tagcagaagg tttcgcttct gggaaaacag ttttgaacat gtttagttat accggcgcct
     3061 tttctgttgc tgccgcaatg ggaggggcaa tcgaaacaac aagtgtcgat ctagcaaaac
     3121 gcagtttaaa aaaaacacag gaacagtttg aagtgaatgg tttggatgtc gatcagcaaa
     3181 aaatcattgt tatggacgta ttcgagtatt tcaaatacgc caaaagaaaa caatttttat
     3241 atgacatgat tgtattggat ccaccaagtt ttgccagaaa taagaaaaaa gtctttcgcg
     3301 tagcaaaaga ttatggggaa ctggtcaagg atagtctgga tattttagca gaagacggga
     3361 tacttattgc gtctacaaat gctgcgaatg tctcgataga acaattccaa acaatgattg
     3421 aagaggaatt ccatgccaaa aatgtttctt ttgaacaact tgctttatat cgtctgccaa
     3481 aagatttcca gacgacggat acctttttag aaggcaatta tctgaaagtg tttgtttatc
     3541 aaattaaaaa taaccattag aaggagcgat gcagaatggt tcgggtaaag ggattgaata
     3601 taggaagcgg tagaccaaag atttgtgcgc ctcttatcgg ggccgtccga gaggaagtat
     3661 tacgtgaagc agttcttgca cgtgaagcag gggccgattt agtcgaatgg cgagtcgatc
     3721 attatcgtga agttttttct tttgatgaag tagcagaaac attggttctt atccatgaga
     3781 tgcttgatgg actgccgtta ttatttacgt ttcgtacctt agctgaaggc ggagaacaag
     3841 acatatcgat ccgtcagtat cgcgaccttt atgaacatgt gatacacaca ggattagtag
     3901 atatggtcga tatcgagttg ttcaaagtag aaagtctagg aaaaagactg atagaggaaa
     3961 tcaagtcttt aaacattcct ttaattatca gtagtcatga ttttaaagaa acaccagcag
     4021 accctgtctt gctttaccga ttgaatgtta tggaacattt tggagccgat atcggaaaac
     4081 tggcagtaac tcccaataat gaacgagatg ttttgcggtt gatggagttg actagacggg
     4141 caaatgcttt tgtctccatg cctattatta cgatgtccat gggaaatctt ggtaaaattt
     4201 ctcgtctggt tggcggaacg acgggttccg ttatgacatt tggttcctta agcagcgatt
     4261 ctacttctgc tccaggacaa ttaccggtca aagcattgaa gcaaattatt caattattaa
     4321 atgaaccgtt gtagaacaag tttgaggtga tattttttgg caaagaaaaa aatagtaaaa
     4381 acgaatgcaa tccgtatggt cgaacaaaaa aagattccct atacagagca cgaatatgag
     4441 tgggacgaaa gccacctaag tgcgtcatcg gtcgctgaac agcttccaga aagccaatct
     4501 cgtatattta aaacactggt tgctgtgggc aatgtgacgg ggcctcttgt tgctgtgata
     4561 ccgggagaag ccgaattgaa tctaaaaaaa ttagcaaaag tttcagggaa taaaaaagta
     4621 gaaatgctcc atttaaaaga tttggaagca acgaccggat acatacgtgg tggctgctcg
     4681 cctataggta tgaaaaaact atttcctact tatttagatc aaattgcaga aagctatgaa
     4741 caaatcattg tctcggctgg cagaagaggg ctacagatgg agttagcacc tcaagatatc
     4801 tgtgcattaa cgagcggaca atttgcagat ataaaacaat aaatcatacg tataaaagaa
     4861 agaaggaggc cgggatgttg tttgtccagt ctccttcttt cttttttata cattatgctc
     4921 tttgcccggc attcattcct gctacatgcc ctgtagcgaa agccgctgtg atattaaagc
     4981 cgccagtata accattgaca tccagtaatt ctcccgcaaa atacagacca tcaatgattt
     5041 tactctccaa tgttttagga taaatttcct ttagagaaac ccctccgcca gttacaaatg
     5101 atttctcaat aggaaaagtt ttgttgattg atagcttaaa ctctttgcat agttgaacaa
     5161 aatcttgaat ttgtttttga gaggcttgtt gtcctgtcag atgatccatt tcaagccgct
     5221 caagaaagaa aactaacaat cgctcaggca atataccatg ccaagcattg accaagttct
     5281 ttttggttgc tttacttttt tctgtcaata tatgaataag ctcttgtttg gtttgtgtag
     5341 ggaaacaatc cagagaaagt gtcactggtt ctcccgtttt ctctaattct ttattgacaa
     5401 aacttgaaca acgcagtgca gccggaccag aaataccgaa atgggtgaaa agtaaatcca
     5461 tcgtatgttc agtcaataca cgaccttttt tatttaaaac gcgtaaagtg atgtcttgta
     5521 aagaaagccc ttgaagtgtt ttctcctgaa tatacggttc atccgatatc aagggggact
     5581 ctgtcgcata aagaggcgtc accgtgtgac ccactcgctt cacgatcttg tacccatctc
     5641 cagtagaacc agtagatgga taagtccgtc caccagttgt taagataatg gcaggagcat
     5701 ggaactcttc gaattccgtg cgtataccgt aaattttatg ttctttgtgg atcaatttct
     5761 ctactcgagt agagaataga agcgtcacat ctagttcttt cagacgattg agcaatgctt
     5821 cgataatcgt ttttgattta tttgttacag gaaacatccg tccatgatcc tcttctttta
     5881 aatgtacgcc ttgtgattca aagaagttca tgatatcgaa attattccat tgcgcaaacg
     5941 tactatataa aaactttcca tttccaggaa tatgtgcaat caaatcatca acaggacgat
     6001 tgttcgtaac attgcaccga ccaccacctg tcatcagtag tttttttcct gcttttttgt
     6061 ttttttcgat caagagtacg cgtgcgcctt gttcagctgc actgatcgct gccatcatcc
     6121 cgcttgttcc agcgccgact acgatcacat catacagttt ttccattccc tcacctcaag
     6181 gaaagtgtag catatctgaa gaaagaaaac agaaaaaaat gttttgagcc aaaagagaaa
     6241 atgagctaaa atgagggaag ataagcaaag gagcgtggaa cgatgacaaa cgcaacagaa
     6301 tatgttcaag caattcaaga aaagctacat caaaaggatc aagggcaaga agaattttta
     6361 caagcaatcg atgaattcat gcccacggtt atgtcttttt tggacactca tcccgaatat
     6421 atcgaaaaga atatcttaga acttttaacg gaaccagaaa gagttattca atttcgagtg
     6481 ccttggcaag atgatgaagg aaactggcga gtaaatagag ggtaccgtat ccaatacaac
     6541 tcagctatcg gcccttataa aggtggattg cgcttccatc ctagtgtcaa tctaagtatc
     6601 ttgaaatttt tggcttttga acagattttc aagaatagtt taaccggtct gccaatcggt
     6661 ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat ctgataatga gatcatgcgt
     6721 ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg gcccatcgat tgatgttcct
     6781 gcgggagata tcggtgtagg agctagagaa atcggctatt tgttcggcga atataaacga
     6841 ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt tggatttttg gggaagtaag
     6901 atacgtacgg aagctactgg ttatggatta gtgtattatg tcaaacatct attgaacgaa
     6961 gaaaaagatt catttaatca aaaaacagtt tttgtttccg gaagcgggaa tgtagccatt
     7021 tatgcaatcg aaaaagtgca agagctaggc gggaaagttg ttacatgttc agattctagc
     7081 gggtacatct atgatccaga aggaatagat gtgaaactat tgaaagaagt caaagaaatc
     7141 aatagacaac gtttgacgga atacgcaaac caacgtcctt ctgctattta tcatgctgga
     7201 gaatctgttt ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc tactcaaaat
     7261 gaaatagacg aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat cgtagcagaa
     7321 ggcgctaata tgccatgtac agtaaaagct gtttctgttc ttcatgaagc aggcgtgtgg
     7381 tattgtccag gaaaagctgc taatgctgga ggagtagcag tatctgcttt agaaatgagt
     7441 cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg atgagcagct ggatcaaatc
     7501 atggaaacga tttaccatac gtgtcgccaa acagcagccg agtacggcga cgaaaaaaat
     7561 ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat ggcagcagaa
     7621 ggtttagttt gaaaaatgaa actttcttgg aaatattgtc agcattttgt ttatttgctt
     7681 gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag taaacctata aggaggagaa
     7741 caaatggcac acattcattt tgactattca aaagtagcac cgtttgtcaa cgaacaagag
     7801 ttagggtaca tgcaaagcca agtaacagca gcccacaacg aattacggga aggtactggc
     7861 gcaggaagcg acttccgcgg ctggatcgat ttaccaacga actatgacaa agaagaattt
     7921 gcacgtatca aagaagcagc taaaaaaatc caatctgatt ctgaagtatt agttgttatc
     7981 ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg acttcttgaa tcacacattc
     8041 ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc cggaaattca
     8101 atcagctcaa catatattgc tgacttgatc gaagtaatcg gtgatcgcga cttttcagta
     8161 aacgttattt caaaatctgg aacaactact gaaccagcga ttgcgttccg tgtattcaaa
     8221 gagcttcttg ttaagaaata tggacaagaa gaagcaaaca aacgtatcta cgctacaaca
     8281 gataaagcaa aaggtgcagt taaagtagaa gcagatgcag aaggttggga aactttcgtg
     8341 attcctgatg acatcggcgg acgtttctct gtattaactg ctgttggttt gttaccaatc
     8401 gctgttagtg gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc aagcaaagca
     8461 tactcaagcg acaaactaga agaaaacgaa gcttaccaat acgcagcaat gcgcaacatt
     8521 ctttaccgta aaggcaaagt aactgagttg ttgatcaact atgaaccagg aatgcaatac
     8581 ttctcagaat ggtggaaaca attgtatggt gaatctgaag gaaaagacca aaaaggcatc
     8641 tatccttcaa gcgcaaactt ctcaactgat ttgcactctt taggacaatt catccaagaa
     8701 ggacgtcgca atatttttga aacagttgtg aaggttgaaa aacctcgtaa atcaatcaca
     8761 attcctgaac aagcagaaga tttagacggt ctaggctact tgcaaggaaa agaagtggac
     8821 ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc atacagacgg tgacgtgcct
     8881 aacttgctag tcaaaattcc agaaatggat gcttatacat tgggttatac aatgtacttc
     8941 tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt cgaccaacca
     9001 ggtgtagaag cttacaagaa aaacatgttc gcacttctag gcaaaccagg ctttgaagat
     9061 cttgctaaag aattgaacga acgcctgtaa gataagggtt tttcgctaga tttaaataat
     9121 cagtgataga aacatgttcg ctttaaaatg aaaactggta cccatttagg tactcaaaca
     9181 tactgcatcg tttattcaga ataaggggct gtgacaagac gtttgtcaca gtcccttgtt
     9241 tgtatagaaa tcgattgatt gccttgctct ttagtttgtt ttaggacttt aagaacagtg
     9301 aggctgactt cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa gcaaaaaagc
     9361 actcacttcg agtgcttttt tgcgatatat tcttagctta agataggcct aatttttctt
     9421 ttacagatga gggtacctta tcgctggtaa ttactttttt ctctatgatc cgcttttcgc
     9481 ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga accattggca gagtcaccag
     9541 aagtaatgac atcgaaatct aactgtcttt tttcaccgtt ttcagcataa gcatctacgt
     9601 tgtatttaaa acctcttcca attacttccc cattatctgc tttgatatca gtttctgcag
     9661 gcattggagc tttgactacc gcataataat ctttacctac ataagtagac tgatagtagt
     9721 cccaaccttt ccatcctata aaagcaatca atatgataac gatcaaccct aaaatttttt
     9781 tcatgaaaga tctccttttt tatttttat
//
LOCUS       DACEOE010000094         9795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEOE010000094 DACEOE010000000
VERSION     DACEOE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.096"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(7..231)
                     /locus_tag="IW774_001848"
     CDS             complement(7..231)
                     /locus_tag="IW774_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ1154194.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(429..2009)
                     /locus_tag="IW774_001849"
     CDS             complement(429..2009)
                     /locus_tag="IW774_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1154195.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2410..3786)
                     /locus_tag="IW774_001850"
     CDS             complement(2410..3786)
                     /locus_tag="IW774_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1154196.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3914..5032)
                     /locus_tag="IW774_001851"
     CDS             complement(3914..5032)
                     /locus_tag="IW774_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1154197.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5174..5608
                     /locus_tag="IW774_001852"
     CDS             5174..5608
                     /locus_tag="IW774_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1154198.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5686..6219)
                     /locus_tag="IW774_001853"
     CDS             complement(5686..6219)
                     /locus_tag="IW774_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1154199.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6313..7491)
                     /gene="mutY"
                     /locus_tag="IW774_001854"
     CDS             complement(6313..7491)
                     /gene="mutY"
                     /locus_tag="IW774_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1154200.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7484..8281)
                     /gene="recX"
                     /locus_tag="IW774_001855"
     CDS             complement(7484..8281)
                     /gene="recX"
                     /locus_tag="IW774_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1154201.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8388..9770
                     /gene="rlmD"
                     /locus_tag="IW774_001856"
     CDS             8388..9770
                     /gene="rlmD"
                     /locus_tag="IW774_001856"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1154202.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
BASE COUNT         2865 a         2015 c         1681 g         3234 t
ORIGIN      
        1 ttctttctag ctttttgatt tttctctttt cttttgtact tttctctttt cattttcagt
       61 tctacgcgtg tctttatttt taactttttc ctctttatgg atgattgatc gtactttttg
      121 tgcaatttgc tcgtttttgt cccagtcttt tacatctaat aatcgacata atcgtaacat
      181 ctcttctttt ttctgttttt ttctttcacg tctttgccat tctgtttcca tgtgtcacac
      241 tctcctttct ttaatcatat gtacgcaaaa taatttttat atttttttga ttagacattt
      301 ctatgatttt aatatagtaa aaagagccgt cacggaaaat ccgtcacggc tctaaacaaa
      361 atatgagagg ctgtttttgt ttggctattc ttattacttg atgcagggca gcttgtgtca
      421 agacttcttt acatcaaact ttttaatttt acattgggat atttatccgc aaaccagcgt
      481 tctgcaaatt ggttctcgaa taagaacaac ggttggtcga aacggtcacg agccaagata
      541 ttgcgacttg aactcatctt ctcatccaaa tcttctggat cgatccagcg agcgattttg
      601 tgtcccattg gagacatgat tacttcggcg ttatactcat ttaacatacg atattggaat
      661 acttcgaatt gtagctgacc aacagctcca atgatatatt cttccgttag atatgtttta
      721 taaagctgaa tcgctccttc ttgaactaat tgatagattc ctttgtggaa ggatttttgt
      781 ttcatgacat tttttgcagt cactttcata aacagctctg gagtaaatga agggagttct
      841 tcataagcaa cttttagttt tccttcgaac aatgtatccc caatctgata attgcctgta
      901 tcatatacac cgatgatatc gcctgctact gctttttcga cgttttctct cgaatctgcc
      961 ataaattgtg tgacattgct taatttcagt tttttatttg tacgttccaa ccagacatcc
     1021 attcctcgtt cgaatgttcc agaacagatt cggacaaaag cgattcggtc acgatgggct
     1081 ggattcatat ttgcttggat cttgaatacg aaacctgaaa attcttcctc ataaggactt
     1141 accatctgat catcttgtgt tttgtgtcca taaggagctg gtgccaattc aacgaaagtc
     1201 tctaaaaaag tctgtacacc gaagttagtc aatgcagaac caaaaaatac tggtgtctga
     1261 tcgccacgag caattttttc ttgattaaat gcatctccag cctctttgat tagttctact
     1321 tcttctaaga cttgttcata taaggaattt tcagttagag gatgattttc gggaagcagt
     1381 ccatcttcat ccaattctac taatcgttca tcctgataat tttccggacg ataaaactct
     1441 acacgattat ggtagatatc ataaagacct tctagccctt tacccatacc gattggccaa
     1501 ttcatcggat aagattcgat atctaatagc tcttctaatt cttctaacaa ttccaatggt
     1561 tcacgaccat cacgatctag tttgttgata aaagtaaata tcgggatgcc acgttttttt
     1621 acaacttgga ataatttctt tgtctgtgct tcgatccctt ttgcactatc gattaccatg
     1681 accgcactat ccacggccat caaagtacga taagtatctt cagaaaaatc ttcatgtcct
     1741 ggtgtatcca agatatttac tcgtttccct tgataatcaa actgcataac agaactagta
     1801 acagaaatcc cacgttgttt ctcgatttcc atccagtctg atttggcaaa attacctgtt
     1861 tttttgcctt ttacggtccc ggcttgacgg atagccccac caaacaatag taattgttct
     1921 gtaatcgttg ttttcccagc atccggatgg gaaatgatcg caaaagttct gcgattgtcc
     1981 acttgttgct ttaattttgg attattcata acctaactcc atttcttcca ttcaattatt
     2041 tatattttct ctaggcacag attgtccgat ctcttaaaga aagacaattt catatagggc
     2101 tgtgacaaaa gccgtgtagc agtctctttt tccgagtaaa cggcgttcta gagacagcct
     2161 atcagcaata agcccaccca agccaaaaat acgaaaagct gtttttgctt ggctgctctt
     2221 attacttaat gcaggatagc tttttcacaa cctttgtcaa ctactacaac tgaatcagta
     2281 tagcatggat tcagcgaatg aaaaagccac caaaccataa gagtcaaata tttttttgtc
     2341 agctgactaa aagaaaagac gtgttgcttg tttattcaag caacacgtct ttcataacca
     2401 agtttccaat tatcttcttg gctcgtcatc ttcgaattct tttctgaagt atcttcgttc
     2461 ttcttcaggt tccgcttctg tttcttccgg atcatgaaaa atgacacgga ttttaaggat
     2521 tcgagaacct tccatttctt cagaaaccag cgtaatatta tcgacatcaa acgataactt
     2581 ttcgccttca tccggaatca tgcctaatgc tgtaatcaaa taaccagcca tcgtatctac
     2641 atcactcata tgcagatcac tgtcaaaagc ttcattgaac tcatcaatca gcatacgtcc
     2701 ttggatgata tattcgtgtt catcgatttt ttcataaaga ttctcaactt catctgattc
     2761 atcatcgatt tctccgacga tttcttctaa aaggtcttct agtgttgcta acccaacgac
     2821 gccgccatat tcatctaata aaatagccat ctgattctgc gtctttttca tttcatataa
     2881 cagatcatca ataaagaccg tttcaggaac gaataaaggc tcctgcatga ttttcttgat
     2941 atctaaatta tcaaagccga atttatgggc agcttttagt aaattctttg tatgaaggat
     3001 tccgactact ttatctttgt cttcgttata aacagggatt cttgaatagt tttcggataa
     3061 aacttcattc acattttctt ccacagtgtc gttgatatca atcataaatg catccgttcg
     3121 tgggaccatg acttctcggg caactttcgt atcaagagaa aaaacccctt ggagcatttc
     3181 taattcttca ttattcaata cgccttcgtt ttcaagcata taacgcattt catctctggt
     3241 catttttgaa tcttcatcat caaaagtcat cggcgtgata cgcgccaata aacttgtgga
     3301 agcagaaagc agccagacga agggcttagc tactacacca atcaagcgta tcagccctga
     3361 agtgaacttt gcgacttctt ctgatttatt taaagcaata cgtttagggt acaattctcc
     3421 aaaaacaatc gatacatagg tcaaaatagc cagcacaatg atatttgcaa tattttttgc
     3481 cgctgtgccg ccacctaata ccggagctaa ccgttccgat aaagtattag ctagtgaagc
     3541 accagacaaa atgttgacca aagtgattcc tacttggatt gtcgatagga aattatttgg
     3601 attctgcaat atttttaaaa gcttttgaga tttcacatct ccttcatcag ctttttgctc
     3661 gattcgattc ttattgatcg aaacaacagc aatttcggaa gctgctaaaa aagcattgat
     3721 caatgtcaaa acgatcaata ataaaatctg cgctaacagc gactgactct cagggtcagc
     3781 attcataatc gcattctctc cttataaaat taatttttag tttacgatag aaaaaaacgt
     3841 ggcactatag ccacgttaaa atggtatcac aaagcaattt tctttgcaat aaaaacaagt
     3901 gtactaaaac tacctacgag gtcgtagcaa ttttcttctt gttttcattt tgcttgtctt
     3961 ctttgacgat tttgattata taggaaatga tcgtcttgca gattgcgtaa aaaggaacac
     4021 ctaagaagat accaagcaat ccggcaatat ttccagctac tagaagaacg ataatgatcg
     4081 ttaatggatg gattttcagt gatttcccaa ttacattagg ataaatgata ttcccatcca
     4141 actgttggac gattagcaca accacacaac aaagtaatgc ttttaccgga tcattgaaga
     4201 ctgttactag aaatgcaggt gctagtccta gataaggtcc taaatatgga attaggtttg
     4261 tcaatcccgc aatgacccca aataagaaag cataatccac accaattacc atatatccta
     4321 aaaatgtgaa ggtaccaacg aataaacatt cgattgcttg tccgctaatg taattagcca
     4381 atgttttatt cagttgcccc aataactcca caatttggtt tcttcttttt tctgggaaaa
     4441 actgttggat gtttggcact agtttttctc cgtctttcaa catatagaac aagatgaatg
     4501 gtgctgtaat gattactacg gttgccgaag cgatcgttcc aacgatcgaa cctaaactat
     4561 tggatactcc actaaggaat tgctgaatga ttgtcccgta agaaatgtct aattgctcaa
     4621 aatatttatt gatatctact tccttgaaca aaggcaattc ggctacctct tttaaccagc
     4681 tttctacctg tttgatgaaa tcaggcatat tagatgccaa tgaggaaatt tgctgtacta
     4741 gacttgggat gacactaagt aaaatccaaa taattgctgc aactaaaagg agcagcacaa
     4801 ttgcgacagc ccatattctt ttgatttttg ctttcatcaa cagcttgaca ataggattca
     4861 ataaatagta aagaaagcct gcaatcagta caggtgcaaa taacgtggaa aaaaatgtcc
     4921 cgatcggttc gaaaataaag tttatttctg atgaaatcat gatcaatgca gctatgatca
     4981 ataattcaag cgaccaaaac attagtttag actgtctaag tttttcaaac aatagatttc
     5041 ctcctttctt tcttactagt atatattata ttcgttaaaa attcttttat tcttttttta
     5101 tttttgaaaa cttgccatac tttttatttg ttttattttc cagtatacta tacgaaagga
     5161 aggtggctag accatgaaag ttgtacaaac aaaagataca atgagcaata tttacttaga
     5221 tgctgtacgg atcaggcatc aagtttttgt agtcgaacag ggtgtgcctt tgtctcgaga
     5281 aatagacaaa gatgaagccc attgtatcca cttcgtcctg tactcagata aaaaagaacc
     5341 gcaaggaact gtacgtcttt tgcctttaga aaacggaaaa atgaaattac agcggatggc
     5401 gattctttct gaatacagac accaagggtt aggaaaaata ttgattgaag aagcagaaaa
     5461 ctttgctaag aatcaaggct ataatacgat tttattaggg gcacagtcca cagctgaaac
     5521 tttttacgaa aaactaggat atacagctta cggcgatcct tttgaagatg ctggcatgcc
     5581 tcatatttat atgaaaaaga ctttataaaa atttatttcg aaaaaagcaa aaaacagcaa
     5641 gcttttgaga aaaatctctc actggcttac tgttcttttt tgtgtttact tacgcgacaa
     5701 ttgcttgtaa cggttatacc aaatatcaat atagccttcc gagaacgggc catctccatt
     5761 attgatccag tcaaccagaa tcttcacatt ttcttttagg atgaagtcaa tatcgttcgg
     5821 gtaattcatc attttgctgt gcatctcata ttcatcgaca tccaatagtc gtttctcgcc
     5881 gtcaggaaat accttgatat ccaagtcata gtcgatatat ttcaaggcct catcgtccag
     5941 caaaaaaggt gaagctagat tacaataata ggaaaccccc ttttctctga tcattgctat
     6001 tacattgaac cagtattttt tatgaaaata aacaatcgct ggttcgcgcg ttacccatcg
     6061 gcgtccatca gattcagtga ctaatgtgtg atcattcacg ccgatcatag agtgttcgct
     6121 tgtttttaat accatggtat ctcgccacgt tcgatgcaat ttgccgtcgt gcttgtaact
     6181 ttggatcgtc acaaactctc cttcttttgg aactcccata gcaacccttc cttaagcagc
     6241 attttgtgct ttgtgtttgg gtgttcatta caccgacatc ataattatta tatcaataaa
     6301 aatgagcttt gcctagaaag gattcctatt gtttcgattt ttctctaact gatctaccag
     6361 tttcatttgg accttaggaa aaacgaccga atcaaatgca tcaggcttca accacttcga
     6421 agttttgttc tcggtaaatt cttgttctgc tccctcggtt gcccgtccgt aaaaaaggag
     6481 aacatgccat ttcagatgac taaaaatatg agtaacttct cctaaatgtc gtgtctgcca
     6541 aacaaagagt tcctgctttt caaatgggag attttgctct tcttctgcta ctaggtcatc
     6601 aaacagatcg atttctggct ttgtttccca ttcttttttc aatcgctcat actcctcttg
     6661 tgtcacttcc atcattggaa atgtccacat attcgccagc aatttttggc tatctctttc
     6721 ttcaaaataa tacgctccag aatgattttg aagagctgca ctgatataat aaacgtcttt
     6781 tggctttgct ttttttgttt ttacaggaaa actcgtttgg attcctcgtt tgttagctaa
     6841 acaaaaggct tggatcggac aagtctcaca ctttggagaa gttggtgtac agatagctga
     6901 acctagatcc atcattgctt ggttgaattc acctggatgc ttttcatcaa tgattttccg
     6961 catcgcttca tcaaagattt tcctgctaga agcttttgcg atgtctgctt caatgcaaaa
     7021 caatctgctg actactcgca tcacgttgcc gtcaactgca ggttctggaa gtccaaatgc
     7081 gatacttgca atcgcgcctg tcgtatatgg tcctattccc ttcaatgagc taatttcttc
     7141 aggcgtttgc ggcatttccc catcaaattc tgacatgatc tgtttagcag cagcttggat
     7201 gttccgggct cttgagtaat aaccaaggcc ctcccatgct tttaaaagct tttcttctgg
     7261 agcatttgct agctcttcaa ttgtaggaaa ccattccatg aaacgataaa aataatcgat
     7321 cactgtgtct actctggttt gctgaagcat gatctcagag atccagatac gataaggatc
     7381 acgattatag cgccaaggca ggtttctttt ttcttgttca taccattgaa tgaactgatc
     7441 ttgaaattct tttgtttctt tatctgtcca tttttcccat tctttattca ctttcctctt
     7501 ccttttccgt aatgaatcgc tggatcatat ccaaatcaaa tcctttttga tataagcttt
     7561 gctttacttt tagatttctt tttttaggtt caaaccttaa atttcttcgc catagtttat
     7621 ctccttgttt gactaaataa tcatattcta tttcttgatc tgcttcttgc ggaagatcag
     7681 ctatcgcttg ttggatcact tcttgagaaa agcctttcgt catcaatgtt tgttggattt
     7741 ttctcaataa ttctttttgg ctttttccgt gattttttcc aaacgctttt tcggctgtct
     7801 gatgtgcatt ttgaacttgg tcttcaaatg tatattggct gagcgcttgt tcaatgattt
     7861 caggtttcaa acctttttgc tgcatctgct gcgctaattt ctttggtcct ttgtcactta
     7921 agcgcatatt cgtgcgcaca taactttccc catagaccaa atcatcgaca aggtccaatt
     7981 cttttaagcg ggctacgatt ttatttcgat cttcttgaag gatttccttt tcttttaagt
     8041 atgttcggat ttctttttcc gatcgcaact gataacttaa gtaattcatt gctaggtgaa
     8101 aaccgaaatc agctccgcta ctcttcttga tctcttggat agtttcttcg tctaattccc
     8161 gtccttttaa taatcgatag cgtactaata catcttcaga tacttgtaaa gcttctcctt
     8221 cagataaatc tacgcgatag aaagggcctt tgcctttact gattctcgta acggttaaca
     8281 taaacatcct cctcgaacat atattctcta tcataccaca gaaaatcccc gtaatcaggt
     8341 attatatgtt agaataaacc acagaaaaaa gaaaaaggga gatacttatg gcagaaacaa
     8401 ttgtcaaaac tggacaaacg gttcctttga aaatcaagcg tttaggcata aatggtgaag
     8461 gaatcggtta tttcaaacgg ttgatcatat ttgttccata tgcactacca aaagaagaag
     8521 tactagtgaa aatcacgaaa gcaactcctc gctatgcaga aggagaatta ataaaagtca
     8581 aaaaaacaag caaagatcgc gtagtggcac catgtcctgt ttactatgaa tgcggtggct
     8641 gtcaattaca acacttagct tatcaagctc aattggattt taaaaaggat ctgcttttgc
     8701 aagcattgga aaaattcaaa ccagcaggct ttcgatctta tcaacttttg ccgacgatcg
     8761 ggatggatga gccttggcat tatagaaaca aagcacaatt tcagttacga aaaaataagc
     8821 agactcaaaa aattgaagct ggtttatacg aagcaaactc tcatcagtta gtccctataa
     8881 cagactgctt ggttcaagaa cctcgaacac aaaaagtaat gaatactgtt gttcaactat
     8941 tgaataaatt tgatgcaccc atctatgatg aacggacaaa cagtggaatc ttccgcacaa
     9001 tcatggttag aatcggtgtt aaaactgggg agttacaagt tgtttttatt actcgcagta
     9061 aaaaattccc gcaaaaaaac gcaatgatcc gtgagatcaa tgaacgactg cccgaagttg
     9121 tttcaatcat gcaaaatgtc caggcacaaa aaacgtctct cgtgatgggg gatgaaacga
     9181 tccacttatg gggaaaagaa gccattgaag aacaaatcaa tgaagtgact ttcgatcttt
     9241 ctcctcgtgc cttctttcaa ctcaatcctc agcagaccga attgctttat ggcgaaggaa
     9301 tcaaagcatt agatgtccag cctaatgaaa cgatcgtgga tgcttattgc ggagttggga
     9361 cgatcggctt aagtgtagca aaacaagcca aagaagtacg gggaatggat accgttcctc
     9421 aagcaatcgc tgatgcaaaa aaaaatgcgg aaagattagg atatcacaac actcgatatg
     9481 aagtcgggac agcagagcaa gttctgccta aatggttgaa tgaaggtttt cagcctgatg
     9541 gtatcatcgt agatccacca cgtacgggtt tggataacgc attgatccaa tcattattga
     9601 aataccctcc aaaaaaactt gtttatatct cctgtaatgt ttctacactt gccagagatc
     9661 tggttaattt aagtaaaaag ttcaagattg aatatttaca gtccgtcgac atgtttcctc
     9721 agactgcacg ttgtgaagtt gtcgtgaaga tgacgagact cgctgactaa atgaaagagg
     9781 ttgtgaaaaa gtctg
//
LOCUS       DACEOE010000095         9440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEOE010000095 DACEOE010000000
VERSION     DACEOE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.097"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..1257
                     /locus_tag="IW774_001857"
                     /pseudo
     CDS             1..1257
                     /locus_tag="IW774_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1364..2395)
                     /locus_tag="IW774_001858"
     CDS             complement(1364..2395)
                     /locus_tag="IW774_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154203.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(2402..3232)
                     /locus_tag="IW774_001859"
     CDS             complement(2402..3232)
                     /locus_tag="IW774_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1154204.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(3229..4035)
                     /locus_tag="IW774_001860"
     CDS             complement(3229..4035)
                     /locus_tag="IW774_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1154205.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(4041..4865)
                     /locus_tag="IW774_001861"
     CDS             complement(4041..4865)
                     /locus_tag="IW774_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1154206.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(4855..5955)
                     /locus_tag="IW774_001862"
     CDS             complement(4855..5955)
                     /locus_tag="IW774_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1154207.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            6133..6918
                     /locus_tag="IW774_001863"
     CDS             6133..6918
                     /locus_tag="IW774_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1154208.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6951..7334
                     /locus_tag="IW774_001864"
     CDS             6951..7334
                     /locus_tag="IW774_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1154209.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            7410..7541
                     /locus_tag="IW774_001865"
     CDS             7410..7541
                     /locus_tag="IW774_001865"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1154210.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            7510..7746
                     /locus_tag="IW774_001866"
     CDS             7510..7746
                     /locus_tag="IW774_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1154211.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(7824..8795)
                     /locus_tag="IW774_001867"
     CDS             complement(7824..8795)
                     /locus_tag="IW774_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1154212.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         2795 a         2167 c         1821 g         2657 t
ORIGIN      
        1 tatcaaacta ttactaggaa tcaaagatgg aaacattcaa atcactcatg tcgagaagaa
       61 aaaaatacat tcaatcaaca gtatcgttgt ttctgccacg ctcattaaaa cgattcagcc
      121 ctgtcctcag tgtaaacagg tgaatcaaca tgggctgatc gttaagaacg gaaagaaaaa
      181 atcgctggtc cttctcaata agtgtgcgaa tcaacggact tatcttgctt tggcaaagca
      241 acggtacctc tgtcgtggct gtcacaccta ttttacggcg aatacctaca ttgttgaaaa
      301 aaactgtttt atcgccaaac aagtacatta taaaatattg gaggaattga ctgaaaaaca
      361 agcaatgacg acgatttcta aacattgcgg cgtttcttgg tcaaccgttt cgcgaacact
      421 cgcttctctg ctccccatga caaacgtgaa aataaattgg ttgcctcgct gttcattggt
      481 agatgaattt cgttcattga aaaatcaagt cggtccctat tctttttctt gtatggatgg
      541 cgatactggg aaattactgg atattcttcc ttcacgtaag aagaaagatc tcatttctta
      601 ttttatgcaa tttgaacgac gtgcacgttt aaacgtaaaa atactcgtaa cagatatgaa
      661 cgcctcttat gctttgctga taaaagagtg ctttcctcat gccaaactcg ttgtggatcg
      721 ctttcacatc gtcaagcacc tcattcgcag ttttaaggat atttgactat gagtcatgaa
      781 aagcttcgac cgaaacgatc caatacaagc caaacactat cgacaagtga aggctttatc
      841 tcgacttctg atcaaacgac aggacacgct tgtttatgat aaatggacaa aatggcgtaa
      901 ttttggttgg gcctatttaa ccgaaagtga agtcgtagaa cgtctacttt ctacttctga
      961 tgaactacga atagcttatg cttactatca agaaattctt caagcttttt atgacaaaga
     1021 agcggacatc ttctttcagc ttgtaaaaac aatgcccaag tcggttccaa gagaactgca
     1081 tcatataaaa aaagcgttta taaactatga atccggcatt cgtttagcct tagagttgcc
     1141 ttactcaaat ggcaaaatag aaaatctgca tacccacatc aaagcattga aacgaatagc
     1201 ttacgggtta ggatgagaac cagaattttc ttaatcaatc atctgatcac ttattaaaca
     1261 taaaaaaaca tctaaagcag aaaatcaatt ctaccttaga tgttcttata tcactctatc
     1321 agtcctattt gacaaagagc cagaaatgtt tgccgactat acactagctt tgttgtgcaa
     1381 aatgctgagt atcaatttcc acagggattg ctagaatatc tttcagctga atgtataagg
     1441 catcgctaat tttctcttcc cgatcgttca accgatccac taaaatttct ttgccctcca
     1501 gcgtttgaaa aacataccgt tgaatgttgc ctaaaataaa tgattccttc catagaaccg
     1561 gaatcttaac cccctgagga accggttcaa attgaatcag ctccggtctt aaataataaa
     1621 tgcagcgatt gcccttagcg gaaaactcgt gggtatactg attcagctca taccattcga
     1681 aacgattata gttgccgata aattccgcaa caaatcggct ctgaggattc tgatacaatt
     1741 ccttcgaggt ggaaacctga gccacccgcc cagcttccat cacaaaaatc cgatcactga
     1801 ttcgcatcgc ctcttcttgg tcatgagtca caaaaatcat cgtcatccct agcgattgct
     1861 ggatagcgcg caaatcccgc tgcagctgtt ttctaattcg ggcatccaaa gcacttaatg
     1921 gctcatccag cagcaggact ttcggttcag tgaccaatga gcgagccagt gccacccgtt
     1981 gtcgttgacc gccagaaaga aactggacat ttttatctgc ctgctccgtc atatccacca
     2041 atgatagcat ctcaaaaacc ttttcctgaa tcacctcagg actcagcttc tttaccttca
     2101 aaccaaaggc tacattttca aaaacagtca tagttggaaa caatgcatac tgttgaaaaa
     2161 caaagccgat attgcgatcc ttcgtaggct tatcggcaat attctcattg tccagcacaa
     2221 tctcaccgct ctttggttgt tccaatcccg tgatacagcg cagcaacgta gactttccgc
     2281 acccgctggg acctagtaaa gtaaccagct cacccttttc aatctgaatt tcatcaatag
     2341 ctaaaatctg tttttcatca aaaaacagat tgagatcatg tatttctaaa aacattttcc
     2401 gtcacaactc ctttaagggc ttctttttcg actgcttctc ttccctcgtc tgcttaaggg
     2461 acagctgttg cttttgtcga taagtcagca taaaggtagt taaagcaatt cccaccataa
     2521 acagcaaata caaaaccatc acagcgctag ccaaatggcc gttttcgttc atccgccgca
     2581 gcatatagat gcgtaccgtc tgaaatcgac cgccaatcaa caggttcgtc agcatgtact
     2641 ccccaaaaac cgaagaaaaa atcatcagag aaactagggt cacaccgata cggatgtttg
     2701 gaaacagcac ctggataata atcgtagaca tcggggcgcc caaggtttct gctgcatcca
     2761 ctaattcctt cagattaatt aagcgcatct ggttgttaag actaagatag acaatcggca
     2821 acgccgtaat aaacaatgca cctaccaaca ctacaaacat tgggatacca gtttcagcat
     2881 acgttcgaag caaagccgtt accaaaatca ctcctggtaa agcatacggc agcaggagca
     2941 gcttttccag ccaacgattc aaacgaggga aatacaaatg aatccaaata atggtgggaa
     3001 tcatcaacaa cagaatcgtc accaagacca cacctgctaa taaaagtgag cgacccaccg
     3061 cagcaataaa ttccctgtcc gtcagcagct gttggaacca ttgcaaggta aaatcactag
     3121 gtaaaagcga ttttgaccat ttagtggagc tggcatacaa aatcgttcca ataatcggca
     3181 gcaagtaata caggacaaag cagaacaaaa tcggttttat taactttttc acgtctgcac
     3241 caccttgcct ttcttttgaa taatcaacat tgccgttacc agcaggacac ctaaagcgac
     3301 tgccaacgca ctgcctaaat taggcttggc gaacacatct cccgacacta aagccgcaat
     3361 tcgaatcgtt aaaagattta cattactgct ggtcagggca tacgctgttt cataggtccc
     3421 catgccatta gcaaataaga tgacaaaggt gctggcaatc gatggtcgca aaaaaggcaa
     3481 agccaccttg aaccaagcat acgctgatga agcgcccagc aattgtgcgg actccagcca
     3541 ttcctttttc acttccttca tgctgggata caaaaacaag acccctaacg gaatttggaa
     3601 ataggtatac gtgatggtca atccccacca agaatacaaa ttgaagtccg ctagaaatgg
     3661 caaaatcatt ttcagcacac ctacattccc caacaaaata ataaaagaga atgccagcgg
     3721 aatcccagca aaattagctg ccaaattgga aacaaaggtg attttctcct gcaatttagt
     3781 cgatagattc attaagcata aacataaaat aaacgtcccg attaacccaa tcacactgga
     3841 gctgacagca atcagtacgc tgttaacaaa ggcctgtgtg taatatttgt tgctgaaaat
     3901 atcactgtaa ttggccaatg tccactggtt agttaaatca ctgtgcaagc tgcacagaac
     3961 cataaaaagc agcggcagca gtaaaaatgc acagaagata aaggaggcag gaaccagaac
     4021 caagcgtttt ttcatctctt tcatcccctc atcaattctt gaatgtcctg cattttctct
     4081 gacggtgcaa ttcctaataa ccagcaaaac aatggagccg ccagcagctg tggtacagtt
     4141 tgttccttca gcttttggcg tttatttgaa atcaaataaa aaggaacttc acgatgtgca
     4201 gccaagctgc cgccatgcag tccgtgcgca tccatcccgt gatccgcagt caccacgact
     4261 tgataacctt tttgcagcca ttcaggaagg taaatagcca ataatctgtc cgcctgatta
     4321 accgctgctg catattcatg agaatcggcg gtaaatttgt gaccgacatc atcaatattc
     4381 atcgaatgta cgaccaaaaa gtctggctga tactgattca ctaaataatg ggcatccgca
     4441 aataaatgcg aatcagggta agtgtcctca ctataaaaga tgccatgctg aatcagtcgc
     4501 tcctcctgat gctggatgcg atcaaccaaa cgattatagg gggcttgatt gtacaactcg
     4561 ctgtaccagt ggtaagcggc ggctcctgtt tttccgccag cttccttaac taattcaaac
     4621 agcgaccact ctttggatcg ctgagcatgg taattactat agatgccatt ttcataggct
     4681 ggaacgccgg tcatcagtac ttcgtataac ggacgtgaat tgctgggcag ctcacttttt
     4741 accttcaata agcttgcttg ctggtgctca actaaatgat tcatgaaccc aagctgggcg
     4801 acggctgtgt cgtagcgaca gccgtctagt ataactacaa tcagtttgtc aagtctactt
     4861 tgcataggtc agtacctccg cctgccaaag ctctggaagc tctacagagg tcttgttcca
     4921 agcttcattg tcttcaactg gcaccgccgc ttcataagca tcctccggca gtaatttttc
     4981 agctacttct ttaggcagct ctaccttgcg aataggtcgc gcatagccct ttgccaaatt
     5041 gatttgccct tcatcagata aaatatattc ccgtgctagc tttgccgcat tgggatgagg
     5101 ggcattttta ttgattaagg ttgtataccc gctaatgatg gagccgtctg ttggcacagt
     5161 tacctcaaaa cgctcggagt caatttggtc acgataattt agtccattga agtcccaaac
     5221 aactgccaca tcgatttcac ctttttcaag atttgctacg ctggcatcaa cggtggacaa
     5281 acggcctttt tctgccattt ttttgtagta attcagacct ggctgaatat ccttttcgtc
     5341 acctccgttg gcaatcgccg cagccaatac agcaaattgt gcttggttgg caatcgtcac
     5401 atcccccacg ctaacttgat agtcaccctc ttgcagctcc ttccagctag tcggtgcttg
     5461 cttcacattc tctttatcgg tgataaagga aatggttccc gtatagctta acaaccaatc
     5521 cccatcatca tcctttgccc aatccggaat ttcctcccaa tagttggtct tataaggtaa
     5581 ggttaactct ttgctttcag ccaacggacc gaagctgata ccgacatcac caatatcggc
     5641 ggttccattt tttccttctg attcaaactt agccaattct tctgcactgg acatatccgt
     5701 atccacgtga cttaagccgt attctttttc aaggtcgttc caagtgccta cccaatttgc
     5761 ccaagtatcc ggcattccca agctggcaat ctctccttct tccttggctt tttctataat
     5821 cgtatctaaa gagtcgtctg ccgcagccga ttgcttcgtt gcttcacctg agccgcagcc
     5881 tgtcagcagg atacttactg ttgctgcaca aaataaaccg atagtcatgt tacgaatttt
     5941 ttcattcatt ttcattcatt cttctcccct ttcgataatt ccattctaac aaagctttgt
     6001 aatgagagaa tcagtttctt gaaaaggttt catgaacaat gtaaaggttg tatatggttt
     6061 gtaaatttcg acgaattccc gttaaccttc taaagctttt gcggtatgat agtagaaaaa
     6121 aggaggctcc ctatgcagta ttatgatcag catctccaca cgtatttttc ctttgattca
     6181 gaggaaaaat ttgaaaacta tttagcctat cagccggagt ttttcgtttc tacggatcac
     6241 tttgatttaa acaatccatc taccggaaaa gatgacattc cggattatcc agcctatata
     6301 aacaaactgg actctttaaa agaaacctat tccaccaaat ttctgcgagg tatcgagatt
     6361 ggcgttgttc caggacaaga agagcggatc gtggattacc tcgctcagca tccttatgat
     6421 ttaaagctgg taagtattca tcaaaatggc cgctttgatt atatggacga tgtggtttta
     6481 accaaagatc ccattcaaac taccaaggaa tattttgatc agatgttgca ggtcttgacg
     6541 gattttccag aaggagatat tttgactcac tttgattatg gattacgccg cttcaccttc
     6601 acagcggaag aattcgcgga gcattttgag gctcagctgc taacgatttt caaaaaggtt
     6661 gttgaacgag aattggcgat ggagttaaat gccaagagct tcttgaaata cggcaacgaa
     6721 gacctttacc gttatgccgt tcctttgtat caaagtgtcg gcggaaagct ctttacctta
     6781 ggctcggatg ctcatgttgc cgaagattat cagctgggct ttaaaaaaat gagtcagctg
     6841 ttggcagact gtcaggtaga aaagttgcta gtcattcaag gaaaagaacg ccgcctgacg
     6901 ccgctgccga ttttgtgatt cctatataat agaagaaagt ggtgaaaaaa atgagcaacc
     6961 tgtgggacta caatcaagag gcacccatcc attatttaat tgcgcgacat tgggatgcgt
     7021 tgaaaattga ggcggtttgc cgatccttgt tagcagccgt tccaaaacaa caactggaaa
     7081 attttttggt ggctgacagc ttgcagcggg aaaaagttca ggcttatttt gcagccttta
     7141 aagatcagcc tttggaatat ttgcatgcgc agtttcatct tttttatcaa gtagccgccc
     7201 cagatgacta caacgattta cgaggacagc tgcaattgac ctttcaagcg gatgaaaccg
     7261 cctataccgt tttattggga atggctcgcc ttggtgatca ggcgaaggtc gagtggcgga
     7321 ttttcgatat ttgatggcag gatcaaggcg tttcagttgc aaaccttcct ctctttcaat
     7381 tagaaaaacc gcagcaggaa gaagtttgtg tgctggtcac cgatgctggt gaaattcgtc
     7441 tccgcttatt tcctgaccaa gctcccctgg ctgtagccaa ctggcgctcc ctagctgagc
     7501 aaggcgttta tgacaacaca cctttcggct cgtgtcatta aggaatttgt tattcaaggt
     7561 ggcgctttgg atggttctgg tgatgaagct gtttccagct atgagggctt tttcgctgat
     7621 gaagtgcatc gcggacttta tcattttaat ggcgccttgg ccttagggga ccacggcccc
     7681 cacaccaacg gcaaccagtt ttttattgtg caaaacacaa aggcgcaggc tgacctgcta
     7741 atgtaaagca agcatacttg gagcttggag gactaccgga gctggatggt cgttatactg
     7801 tttttggaca ggtataccaa gggttagaaa tcgtagcagc gatcgcagct cagccgacaa
     7861 acagtgaaga tgtaccgatt gagccagtgt tcattcgaca aattaacttc gaaaaaacat
     7921 cgtaaaaaga cactcactga cttcagcaaa agctctattc ttgagctggc tgcagctagt
     7981 gagtgtcttt catttatcca taagataaag cttggattca ataaataaat aaatgttttg
     8041 gttaaagctg tgaccagcaa atgaggaacc gtgcgagcca gttgatctgc caatgcaaac
     8101 atttctttag gtgaaggcgc tggcgtgtct gccaacggat actccagtcc caatgcttgg
     8161 taaccgtgca taccatagca gtggtacggt agtaattcta acggtacgaa ccgatcaaga
     8221 tctgccacaa aacaagcaat cgtcagcagc tcggtcaggt tcatattgat acctggaacc
     8281 acaggaactc gaattcgcac ttcgcagtta ggatactcaa ttaaaaagcg acgaatattc
     8341 ttcaaaattt gttggttatc taagccggtc cattgtttgt ggagcttgct ggtaaacatt
     8401 tttacatcca caaacaatgt atccacatag ggagctacct tttggatttc attataagca
     8461 ccacaaaagc tggtttccag tacggtattg attccaatgt atttcgattt ttgcaaaatt
     8521 tctttagcaa aatccgcctg aaccagtgcc tcgcctccgc taatggtcac tccgccatcc
     8581 gagtgaaagt aaaaaacagc atccttctca atctcatcca ttacctcttc agcggtcatg
     8641 gtctggccaa atcctttttc aatctgcttg ctttgtgatt cgggaatcga acaccaggca
     8701 cagcgtaatg ggcagccctt taaataaaca acggtccgta aaccagcacc atcatgtaga
     8761 gaaccctgtt ctattcttaa tactgttcct tccatataga tttttgaatt acacactagt
     8821 ttaaactaaa acacacaagg aaaaacttac catttaatca taaaattagc tcatataaat
     8881 atactaggat gtaaaataga ctggtcaaaa tctggtcaaa aaagaggact aatacaggta
     8941 gtcctctttt ttgttatttt aatattctaa atcatttttt attggtttat tcccttattc
     9001 gatgtagact caaatcacta tatcactaga tatcaaacgg cgtcatacgg aatcaaaaac
     9061 atcaaatttg acctgttaaa tggtcattga ataacattca aaatcaataa aacggagtca
     9121 gacacggagt caaaaacgct gagagtaaaa aatatataca gccttgtagg taaagtacca
     9181 cagggctgta tattttttat ttcagattac cttaaatgat tccatttact ttttatctca
     9241 ttttttccta ctagttagta tcaaatactc tcctattttc tcaataaact atttttggat
     9301 acatggtatc tgaaacagct aaaaccttgt cattacagca aaaaatcacg ttatccagac
     9361 tgtagacaaa gtccatttta tggatgatgt ctacagtctt ttcttttata atagatttat
     9421 gaaaacgaat gcaaggagtg
//
LOCUS       DACEOE010000096         9427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEOE010000096 DACEOE010000000
VERSION     DACEOE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.098"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(259..1023)
                     /locus_tag="IW774_001868"
     CDS             complement(259..1023)
                     /locus_tag="IW774_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1154213.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(1134..2003)
                     /gene="mdcB"
                     /locus_tag="IW774_001869"
     CDS             complement(1134..2003)
                     /gene="mdcB"
                     /locus_tag="IW774_001869"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ1154214.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(2028..3104)
                     /locus_tag="IW774_001870"
     CDS             complement(2028..3104)
                     /locus_tag="IW774_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1154215.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(3128..3721)
                     /locus_tag="IW774_001871"
     CDS             complement(3128..3721)
                     /locus_tag="IW774_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ1154216.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(3725..5401)
                     /gene="mdcD"
                     /locus_tag="IW774_001872"
     CDS             complement(3725..5401)
                     /gene="mdcD"
                     /locus_tag="IW774_001872"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ1154217.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(5391..5690)
                     /locus_tag="IW774_001873"
     CDS             complement(5391..5690)
                     /locus_tag="IW774_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ1154218.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(5792..7438)
                     /gene="mdcA"
                     /locus_tag="IW774_001874"
     CDS             complement(5792..7438)
                     /gene="mdcA"
                     /locus_tag="IW774_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ1154219.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(7445..8356)
                     /locus_tag="IW774_001875"
     CDS             complement(7445..8356)
                     /locus_tag="IW774_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1154220.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            8476..9333
                     /locus_tag="IW774_001876"
     CDS             8476..9333
                     /locus_tag="IW774_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1154221.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
BASE COUNT         2651 a         2049 c         1685 g         3042 t
ORIGIN      
        1 ctaaactaaa atagtgcttc ctatttttcg tttggttgtt cttattatcg cagatgcaag
       61 cttttaaaca ccatttagtg gagaggttgt gacagagatg ttcagccttg agcgataaga
      121 agcgattttg aaaaatcgct tcccatattt tacaaaatcg tgccttattg ccgataggct
      181 atctctggca caccgtttat tcagaaaaag gagctgcgac aaggcttttg tcacaactcc
      241 gttcataaat atttcctgtc aatccagcat aatcggctga ataaaatcgg cttgccatct
      301 tttttctttc tcagaaaaat caaaatacca tatctgagaa aaatactgtc gttgtgtctt
      361 acttcctgag atcaatcgcc catcttttcg atcgactgaa taatcacagc atctgaaatc
      421 aattcggatg gcaaaactat tttcgtggat ctgtcgataa tttcctactt cctgaatagt
      481 tattttttta gtatgatttc ttacaccgtc tgctttgttt ttttgcagaa ctttctgatg
      541 ttcggcaaac agcttgtctg tataacagct tctggctttt tcaggagttt cttcttccca
      601 agcggattgg atcttcatga atatcttttg gatttccaga aactgtgctt tcttttcttt
      661 agccgttcct gacatgcggg acaatatctc cgctttcagc cttttctttt gtcttttatt
      721 cttgacgata gaaaaagcag aaacccctcc tactgacaaa acagctaatg caagaaatcc
      781 cgtttgctca taactagaag gacctgtacc tggaccataa tacccataag aatctctgcc
      841 gtaagaacga ctgccaaaat aactgccccc atatgaacga ccaccaaatg aacctctgcc
      901 cgaacttcga ccgctagatg tacctccacc atgaccaccc gcgacagcga atccttgggt
      961 cggcagagca aacaaacata ggcccactaa caaaatgatt gctaaataat tgattttttt
     1021 cattgtcttc ctcgttatct tttattacaa aaaactattt ttatgatagc cttgctaaaa
     1081 ggaagagtca ataaccagat gcataaactg tttttttagc aattttgtct tatttagtca
     1141 ttgagtaaca tcggacaaat gtatgtgtcg gcagtccata attcctctat cttcagcatg
     1201 aacaagcttg cagacaagag atctgcgctt cctccaggag agatttggtt ttcctgacaa
     1261 aagtgcaaca gtgctgtaaa tggacgattt ggtaaagcac tttgactagc agcatgagca
     1321 agtttttgca tttccactaa cgttttctga tcactgcgat gcaatacgca tgtgtcatct
     1381 agcgaactca tcaaaactaa caacatatgc atttgtttct gtacatatga atcattatct
     1441 agctccttag ctgcttgaat tgcaagcctg atatgcggat accctaatat cgcttcttca
     1501 tatgcaccat ttacgccata tttttgcttc accctatgac catgagtggt ttttttatga
     1561 cgatattttg tatcaggaaa agcagcaagc ttgctgacat catcaagtat tttcgataaa
     1621 gaaaaaggct gttccatact gattttttgt gcaatgacac tgatcaatag ccccattgcc
     1681 caaatcgctc ctttatgggt attgatgcca tttgtagcaa tcagcattgc cttttccgct
     1741 tttcgcccaa tctcagcaat tctttctctt aactcctgat caatagcatg atgccatgct
     1801 atttgagcca tttcttcaaa aaaaggagtt agcgtcttag cagacttaag aaataaagca
     1861 tagtccatat cggaatgtgc acccgcatcc agtgcatcca ctaatccagg ttttggactt
     1921 aatgttactt ctgcgatcaa tgcttcctct accaacgcag cgagttgctg agtcactgct
     1981 ttttttcgtt cgtcatttat catgacactc actccttctt cctactatta cttgctgaaa
     2041 ccttttttga tagctaccca aatgatatga agtaaccagc ttaaagcaaa tgcacaactt
     2101 gccgtcatta gataagctac aagaatcatg actcctgaag gtaactgcag ccaactatct
     2161 ttaaacaata ataacagtac ttggaaaaca aatacattgg ctaaatacgt acgataagca
     2221 taaacagaca accatttgaa caatggcaat tttggcgaat cgtttacaat cattttggaa
     2281 gcgcctatga agacagcaaa gatgatgact aaactgtata aactttggat cgtattcaag
     2341 taaggtgcat tcgcaaagct caagtctcct ggatgtgcga gcaatgaacg gacagaaaag
     2401 aacgcgataa ctaaggcaac aggtaagaaa agataacgta ctctgtccaa atattgatag
     2461 actttttcgt gataaacaaa tgcggctacc ccataaatca tgaaactagc aacaaaccga
     2521 tctaataagt accagctgtc atgataaggc ccagtcaata cttgtgtttg atagaacaga
     2581 taccatgcga catataaaat cgttgaacca atcaatactg gccatacata ttttttatta
     2641 gcaaatactt tgtaaccgag ccaaacaaaa aacggcatga acaattggat ctgcagcatc
     2701 ataacggtat accataaatg aggcgctcca tcacctgtga catatttcag taaaaagctt
     2761 gcagtatccg tatacggagt ttgttgttgg actgctggaa atagccaaag ataagccgtc
     2821 gtccatagaa gatacggaac gactagttca aaaaactttt ctttcaaaaa ttcccaataa
     2881 gacaaatgct gacttgtttt taccatatta taaacaattg caaaaataaa cattggtgct
     2941 gtgtatttaa cgatgacgta aaaggtacca agaaaagctg tatttgttgc gctgtggctg
     3001 tttgccaaag caaagctcaa tatcgtttgc atcataacag ccgttacagc tgatactttc
     3061 tggaagtcac ttacgttgat tttgattttt ttgttttcaa acatctcttt ctctctcttt
     3121 ttctttatta ccaaagttct ttggttaatt taggtccttc gactgttttc aatagaattt
     3181 ttttctctgg ctctctcaaa tactctttta gtgaaaaacc gccttgccga gtcactactt
     3241 gggtatcgaa ttgctcaaaa aaagcaggat gcgattgaat tacctgaatt ggcaattcaa
     3301 tcggtgaatt tgcgtacagc agcaaatcaa aatcactggt ttcttttact gccggaatcc
     3361 cagtcgataa ttcaaatcct aagctgccgc cgattcccca tttacatcct gataatatgt
     3421 ttctagcagc atgaaattgc tgataaacag gataatccat gattttctct tgtctatacg
     3481 attcttgttc gatgatgtcc cacggatgaa tgacttgttt gacggcatgt tttgagattt
     3541 ctgttgacca tctctgattg cgggcaggtc cacgtaaacc aatcggtagt atttctgcgt
     3601 gatccgtcga cgcacgacga actgttccga acatcttttc agttagaacg acccattcag
     3661 gcaaggtgaa cccttctatt tcatgtttat caaaaaggac aatatcatgt gcttgaatca
     3721 ttttttatgc atcccattct tcgtccattt tggcacggac tttatttgtt tcgacacgtc
     3781 cgaatgtttt ggctgtttcg tttgtgtatc gatttgtcag catcgtgtca tttgtattgc
     3841 ggacatcttg aatggcttca ttgattgctt cgatgacttt gctgacattt tgttctgaag
     3901 cagcttggtc ggtgatatct tctaggaaat gatagatcgc tcctagtttt gtataattat
     3961 tgatatcgta agcgatagct ggaacttttt cagcagctgc ttcgatttca gcaaccgaac
     4021 gttttgtcac acgtgcagca ctttctttag acatcgcttg gacggtaatt tctttattgt
     4081 ttaaagcaat caagcggctt gattgtaatc catgtgctaa aaatgcacct gaaatagcat
     4141 ttcctgcaat caatccaata actggatgtc cagcttgacg gagttttgca taagcagcag
     4201 cactattagc caaagccaca tggataccga tcaattcttc gttatagccg tatgcttggc
     4261 ttggtacatc gatcacaacg atgattggcc gtttcacttt tttgtcttta tcttcttcgt
     4321 aaacgtgatc caaaatttgt cccatgcgga aaccttcaac taaaccaact tcaccgtttc
     4381 gtacacggta catcgggttt ttaggatcag gtacgattga tgtcacgatg gcttcttcta
     4441 tgaatttact tgatttacca taatacacag tactaataga agaagttgga tttttcatgc
     4501 cagttagtgc cgcaaaccaa tcgtaaccaa cacttgctgc agctggttca tttccttctg
     4561 ctacttctgg taagtcaatt gattttgctt gatggttagc aattgcttca ttgtaatcgt
     4621 caatggccat tggttttgaa agatctaaag aatcaagtaa tgataaataa aatgctgagc
     4681 tttcactgcg atgcgtgtct tttcgttcgt ccattgctgc gatgacagca gctttgattg
     4741 cttctacact atcagtcact aattcatcaa tgatttttga ttctacccgt tgacgagatc
     4801 ctaatgtatt ccaaatcaag tctttgtcac tagaatcaaa ttctcgaacg cctgcttctt
     4861 gttcaataac ttctggtcca ttcaaaccga ctctcgcttt ttttgttgcg attaaataac
     4921 tcattaatgc tgaagtaatc gacataccgc caaatgatcc tacacgtcct ggaatcaaac
     4981 cgatgactgg tacatattgt tttagagcaa tgattacatt tccgatttct gaaatagata
     5041 acagtccgta gtttgcttct tgcaaacgaa caccacctgt atctagtaca atgactggaa
     5101 agatctcatt tcctttttta ttttcttcta acacatgttc aagtgcagca acgatttttg
     5161 caccggaaac ttcaccgata ccgccacctt ggaattttcc ttccatagcg attactactg
     5221 cttgtttgcc attgatcgtg ccacggctga caacgatccc gtcatcactt tcagggacga
     5281 ttccttgtgc tggtaaactt ggcgcgatga tttgatcgaa tggatcaatc aattcacgtc
     5341 cattgtcaag tagagcgttc actcgttctc tggcgtttaa ttctacaaaa ctatttttca
     5401 tctgaaagtt cctccattgc ttgcgtcaat cgtaggtaca caactcctgg tgtagcaccg
     5461 aagtcattga tcacgatatc tgctgaaatt ggataacgat caaaaaatct cgttaataca
     5521 ttgttccata cttcgccgaa gccatcgctt cctgtacaaa tttttacagt agattctgtt
     5581 ttttctgttg gaaacatcaa gatttccaaa tctcctgaac cgaccactcc tacatggatt
     5641 ttcttattga tcggctgttt cgttggatat ttataagcta attgttccat ggatctctcc
     5701 cctttttgta aaacagagca tacacgttct gcttattcgt ctttcttctt ccatctgggt
     5761 aaatggcgac aaaaggtttt tctcgccaat tttatgacca agttctgaat ttcattggtg
     5821 gttcatacag gccatctgac cattcgatca aatcatcgat ggtttgtgca gctaataatg
     5881 aacgtttcgc ttcactacgt ttgatgccta gatcttctgg taatttcacg atgccggctg
     5941 cacgcaagct atctagtgat tctttcgtgc tagtcattcc aagaggtgta acaccgccaa
     6001 ttgctgcaat tgctgcttgt cgttcttctt ttgtttttgc tttgtacaaa taagcaattc
     6061 cttcttctgt cacgatatgt gtagtatctt cgctataaat catcactggt gcattggcta
     6121 agcctgcttg tttttgaaca gctagtgcat ctaattcttc gacaaagact ggttttttat
     6181 ttgaaccata tgtttcaacc atttgaacaa ctaatttttg gcctttccct aatggatcat
     6241 cattgtcgcg aagtgattgc catgctggtg tagaatgacg acgaccgcca ggattatgtc
     6301 ccatgttagg cgcaccgcca aaaccagata aacgtccttt tgttacggta gaagagtttc
     6361 cttcgtaatc taattgcaag gttgaaccga taaacatatc gatggcatat tgtcctgcag
     6421 cttgtgataa tgttcggtta ctgcgcatcg tgccgtcttt accgacaaaa aagacatctg
     6481 ggcgagcagc aatatatttt tccataccaa cttcgccacc aaaactatgg atgctttcaa
     6541 cccaacctga ttcgatggct ggaatcatcg ttggatgtgg gttcaatacc cagttcttcg
     6601 cgattttccc ttttaaccct agtgattcac cataagttgg cagtaacaat tcgatagctg
     6661 ctgtattgaa tccaataccg tgattcaaag attgtacgcc gtgttctgcg tagattcctt
     6721 tgattgccat cattgccatt agaatttgca attctgtgat gttttgagga tctcgtgtga
     6781 ataaagcttc taattgataa ggtttatctg ctttcacgac aacgtctacc cagtcaccag
     6841 ggatatctac acgtggtaaa gaatcgacaa cttcgttgac ttgaacgatc acgataccat
     6901 ctttgaaagc tgcagcttcg atgattgttg gtgtttcttc cgtattgaaa cctgtgtaca
     6961 agttgccgtc tttatctgct ttatctgcag ctactaaaac aacatttgga atcaaatcga
     7021 tgaataagcg tccataaagt tccaaataag tatggatatc acctaatttc attgtgccgt
     7081 cttcaatcat ttgggcgaca cgcaagcttt gtgcaccagc ataagagaaa tcgatcttgc
     7141 ttgcgattcc tttttcaaaa atattcaaat gttctggtcg tgagatacta gacatgatca
     7201 aatgcagatc attcactttt tctgcatcta ctttttcaag actttctgaa agaaaacttg
     7261 cttgtttttg attgtcgcct tctaatacaa ctttgtcgcc gggagcaatc agtttttcca
     7321 ttacttctac gattttttca gtttctacat acttgccatt gagcaagttg ctgatttttt
     7381 ccaatcgctg cgcttttttt tcgcgtttag aagcccagtt gcggtttggt ttttccataa
     7441 ttagtcatct ccatttttga tatagaaacg tggcgtcatc aataccatgt tggtctagat
     7501 tgatcgtacg gatcccttta tctacaccaa attttgtatg aatcagcttt gtcaaagtat
     7561 tgccgggggg aaattcaatc gtcagatcca ttcctaattc ttttgccaag tccatcattt
     7621 ggttccactg cacagggaaa gctaggtttt ccaacaaatc tttacgaatc tcttctgcgt
     7681 cctgagtggc tcttccatcc gtatttttta tatacaaaca tctagcatca gatagatgga
     7741 cagactgcat atacggcttg aatttcttga ccgtatcatt catcaagaga cagtgagatg
     7801 gcacagggac tttcagcagc ttcgctgttt gcgcttggtg ccgtttggct tctgccaacg
     7861 taacggataa accttcgata ctcccagata acgtatgttg catcggacaa ttttcatttg
     7921 acagataaac tggatggtca ggatcaaatg tttttgctac aatttcctca gcttcgcttc
     7981 tcgtcacacc tactactacg cccattgcat aaccgcttgg ataagcttct ttcattaaga
     8041 ctgcgcggtg atagactagt tccgctgcat tctcaaatct caaagatcga caagcaacag
     8101 ctgcacaaaa agcaccaatc gaatgtcctg ctgcaatatc aggctgaata ccgttttttt
     8161 ccatttcatc taaataaaat actgcttttg tcagtaatgc tagctggata aaaaccgtat
     8221 cttgatagtt ttcttcttta tcaactaacc gataccccgt cacattttct aaacgttcca
     8281 tgtacatttc agggatatcc gctaacatcc ctaacttctg actgccttgt ccaggaaata
     8341 agaaacctgt actcatgagc ctcgcctcct cagcacttac tttttccact gctatcttag
     8401 tcaatttctg ctataataaa aaatgccaaa aaaagaaaaa accataacaa aaaggtatgg
     8461 atgggatgga taattatgaa attgcttcat ctgcagtatt ttaaaaaagt attcgaaaca
     8521 aaaaatgtta cccaagcagc caaagaatta tttatttctc aacctgcctt gagccgtgcg
     8581 atcaaacatt tggaaaacga attaggtgtc cctttgtttt accatagcgg cagaaacatt
     8641 gagacgaccg tttatgcaga agaattttat ccatatgcag tcaagacatt agacacatta
     8701 gaagaaggaa tcaaattatt gtcttcggtc aatgagaaga tcgttacttc cgtcatattg
     8761 tatttagagg tagcctctgt ttctattccg aacttggtcc gaatttttat ggaaaagcat
     8821 cctgatatcc aattaacgat catgcaacat catttaccag atgatattac cgatcctgta
     8881 ctgtacatca cttcggagat gaagcctggc atgaccaata ttcctatcat gaaagaacct
     8941 gtttatgtcg ccattcctaa gaaacatcct cttgctaaaa aagaacatct gcaattagaa
     9001 gatatccaac gcacccccat cttgatgctg tcaaagagca acgccttccg tcatacactg
     9061 gatgcagcac ttgaagcgaa aagtatcgaa ttgacgatca gttcgacgac agatgatcct
     9121 gcgacattac gttctattgt caagcaagga ttagctgtta gctttttccc aaaagtttct
     9181 tggtcttacg ataaaaatga tcctttcgtc ttgcgggaaa tcaaagattt gcccttgaca
     9241 cgaacaattt atttatcttc tagcttcaca gaagatcatc ctttgatcaa aatgattgca
     9301 aaaacgatgc gggaatttta tttaggaaaa taaaaaaaaa agagctggga caaaattctt
     9361 gtcgcagctc ttttttcgaa caaacggtgt tccagaagta atttcttaga aaataagccg
     9421 aaatttc
//
LOCUS       DACEOE010000097         9421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEOE010000097 DACEOE010000000
VERSION     DACEOE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.099"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            198..2192
                     /gene="uvrB"
                     /locus_tag="IW774_001877"
     CDS             198..2192
                     /gene="uvrB"
                     /locus_tag="IW774_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1154222.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            2202..5018
                     /gene="uvrA"
                     /locus_tag="IW774_001878"
     CDS             2202..5018
                     /gene="uvrA"
                     /locus_tag="IW774_001878"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1154223.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            5281..6546
                     /locus_tag="IW774_001879"
     CDS             5281..6546
                     /locus_tag="IW774_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1154224.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            6550..7281
                     /locus_tag="IW774_001880"
     CDS             6550..7281
                     /locus_tag="IW774_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1154225.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            7416..8300
                     /gene="rapZ"
                     /locus_tag="IW774_001881"
     CDS             7416..8300
                     /gene="rapZ"
                     /locus_tag="IW774_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1154226.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            8297..9295
                     /locus_tag="IW774_001882"
     CDS             8297..9295
                     /locus_tag="IW774_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1154227.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            9329..>9421
                     /locus_tag="IW774_001883"
     CDS             9329..>9421
                     /locus_tag="IW774_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation regulator WhiA"
                     /protein_id="HAQ1154228.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRM"
BASE COUNT         3117 a         1579 c         2136 g         2589 t
ORIGIN      
        1 gactgtaagc tcgaaaaaat gaaaaaaatg cgagtttcta acattttatg ttataaaacg
       61 taacatgtat aataagataa aaaagtggcg aaagtatgtt cggtgtggta taatttttta
      121 gtaaggaatg cctttgcttt ccttttcttt ttattggtcg taaaatgaaa aggaatgaat
      181 gaaaaggagg acgaagaatg attgagagag atacctcccg acactttgag cttgtctcga
      241 aataccaacc ggctggtgat cagcctgaag cgattaacca attagtggat ggcgtagtcg
      301 gcgggaaaaa agcacaaatc ttattaggtg cgactggaac agggaaaaca tatacgattt
      361 ccaatcttat tgagaaagtg aataaaccaa cactgattat tgctcataat aaaacattgg
      421 ctggtcagct gtacggggaa ttcaaggaat ttttccctaa caatgctgtt gaatattttg
      481 tttcttatta tgattattat cagcctgaag cttatgtgcc ttcaagcgat acgtatatcg
      541 aaaaagattc aagtgtaaat gatgagatcg acaaattacg acattcggct actagttcat
      601 tattagaaag aaatgatgtg atcgtcattg catccgtttc atgtattttt ggattaggtt
      661 cgccttttga gtaccaaaaa caagtagttt cgattcgaca gggagcagaa ctagatcgca
      721 atcagttgat tcgtgactta gtcagtatcc aattcgaaag aaatgatata gattttcagc
      781 gcggtcgttt tcgagtgcgc ggtgatgtcg tggagatttt tccggcttca agagatgaac
      841 gtgccttacg tgtggagttt tttggagatg aggtcgaacg tatccgagaa gtcaatgcac
      901 tgacaggtga agttttagga gaaacggaac acgtggcaat cttcccggcg actcactttg
      961 tgacgaatga tgaacatatg gagcatgccg tagcgaatat caaagcggaa ttagaacagc
     1021 gtttaactgt cttgcgaaat gaaaacaaac ttctggaagc tcaacgttta gaacagcgaa
     1081 ccaattacga tattgaaatg atgttagaaa tgggctacac gtcgggaatt gaaaattatt
     1141 ctcggcatat ggacggacgt aaagaaggtg aaccgcctta tacgctattg gatttctttc
     1201 ctgaagattt tttgatcgtg gcggacgaat cgcacgtaac catgcctcaa atcagaggaa
     1261 tgtataatgg tgaccgagcg agaaaacaga tgctagtaga ttacggtttc cgtttgccat
     1321 ctgctttaga caatcggcca cttcggttag aagagtttga aaaacatgtc aatcagatca
     1381 tttatgtctc tgcgacgcca ggaccatatg aacatgaaca aacagatacc gttatccaac
     1441 aaatcatccg tccgacaggg ctgcttgacc cagtgatcga agttcgtcca atcatgggac
     1501 aaatcgatga tctagtaggc gaaataaatg aacgagtcga aaaagaccag cgggtgtttg
     1561 taacgacatt gacgaaaaaa atggcagaag acctcacaga ttatttcaaa gaattaggga
     1621 tcaaagtcaa atatttgcat agtgatatca aaacgctgga acgaacagaa atcattcgag
     1681 atcttcgatt aggtgaattt gatgtgttag ttggaatcaa tttgcttcgt gaaggattag
     1741 acgtacctga agtttccctt gttgctatct tggatgcgga taaagaaggc tttttacgta
     1801 gtgaacgttc cttagtccaa acgattggac gagctgctcg gaatgaagaa ggtaaagtaa
     1861 tcatgtacgc agacaaagta acagattcta tgcgtctagc gatggatgaa acgtcaagac
     1921 gtcgtacgat tcagcagaag tacaatgaag aacatgggat cgttccaaaa acgatcatta
     1981 aagaaatccg agatttgatt tctattacga aagaatcgga agatgataca aaagaagcgg
     2041 tacaagtctc gtacgaagaa atgacgaagg aagaaaaaga cacgttattg atgaaattag
     2101 aaaaagaaat gaaagatgcg gcaaaagctt tggattttga aacagccgcg aatgtgcgag
     2161 atatgatttt agaattaaaa gcttcaaagt aggaggacaa catggctaac gataaaatta
     2221 cgattcatgg tgcacgtgcc cataacttga aaaatataga tgtaacgatt ccacgagata
     2281 aattcgtggt cgttacaggt ttgtccggtt ctggaaaaag ttccctagca tttgatacgc
     2341 tgtacgctga agggcagcgg agatacgtag agagcctttc tgcttatgct cgtcagtttt
     2401 taggacaaat ggataagccg gacgtagata gcatcgacgg attgagtccg gcgatttcta
     2461 ttgatcagaa aacaacaagt aaaaatcctc ggtcgacagt aggtactgtg acggaaatca
     2521 atgactattt acgcttgttg tttgcacgtg taggacatcc gatttgtccc aacgatcata
     2581 tcgagattac cagtcaatca gtagatcaaa tggcagatca agtaatggct ttgcctgagc
     2641 gcacaaagat ccaagtgttg gcaccgatcg tggtaaagaa aaaaggacaa cataaaaaaa
     2701 tattcgaacg aatccaaaaa gagggatacg tccgcgttcg agtcgatggt gagatctatg
     2761 atttaagtga agtgcctgag ctggagaaaa acaaaaaaca tgatatcgcg atcgtcatcg
     2821 accggatcat tgtgaaagaa gggattcgtt ctcgcctatt tgattcttta gaggcggccc
     2881 tgcgattagc agacggctat gctctggttg acgtgatcgg tgaagaagaa atgttgttta
     2941 gcgaacacta tgcctgtcct tattgcggat ttactgttgg cgaactagaa ccaagactgt
     3001 tctcattcaa tgcgcctttt ggagcttgtc cagactgtga tggtttaggt gtcaaattag
     3061 aagtcgatca agatttagtg attccagata gaacgaagac acttagtcaa ggtgctatcg
     3121 ttccatggaa tccaatcagc tctcagtatt atcctcaaat gttagcgcag gcttgtgaaa
     3181 gtttcggtat cgatttagat acaccattcg aagaattacc ggaagagcat caggagatca
     3241 tcttgaatgg gacagatgat cttttccatt tccattatga aaatgatttt ggtggcgttc
     3301 gcgatgtaga aacgactttc gaaggggtat tgaagaatat cgagcgtcgt tatcatgata
     3361 cgaacagtga tttcacgcgt gagcaaatgc gtctttatat gacagaatta acttgtcaga
     3421 catgtaaagg ttatcgattg aatccccaag ctctatctgt tcaaatcaat ggaacgaata
     3481 tcgggcaaac aaatgagttg tctattcaaa aagcagtaga tttcttctca aacttaacgt
     3541 tatctgaaca agagaaagta attgccaaac cgattttaaa agaagtaaat gacagacttt
     3601 cattcttgga aaatgtggga ctagactatc taactttgag tcgcgcttcc ggaacattat
     3661 ctgggggaga agcgcagcga atcagattgg ctacacaaat tggatcgaat ctttctggag
     3721 ttttgtatat tttagatgaa ccatcgatcg gattgcatca acgcgacaac gatcgactga
     3781 ttggctcatt aaagaaaatg cgtgatcttg gcaacaccct cgttgtagtc gaacatgatg
     3841 aagatacgat gtgggcagca gattatttga ttgacgtcgg acctggggcc ggagaacaag
     3901 gcggacaaat cgtggctgca ggcactcctg aagaagtagc taatgatgaa aattctttga
     3961 cagggaaata tctttcaggg aaaaaatcaa tccctgtacc aaaagaacgc cgtaaaggta
     4021 atgggaaagc aatcaagatc actggtgctt cagaaaacaa tctgaaaaat attagtgttg
     4081 aatttccatt aggagaattt gtagcagtca caggtgtgtc tggttctgga aagagtacat
     4141 tggtcaatag tatcttaaag aaatcgttag cgcaaaaatt aaataagaat tctgctaagc
     4201 caggtaaatt caagacaatt tccggctacg aaagtatcga aaagatcatc gatatcgatc
     4261 aaagcccaat cggccggacg ccgagaagta atccagcgac ttatacaagt gtatttgatg
     4321 atatccgtgg gttatttgct caaacgaacg aggcaaaaat gcggggttat aagaaagggc
     4381 gttttagttt caacgtaaaa ggcggtcgtt gtgaagcttg tcgcggggat ggaattatta
     4441 agatcgaaat gcactttttg cctgatgtct atgttccttg tgaagtatgt catggtaaac
     4501 gatataattc tgaaacatta gaagtgcatt acaaaggaaa aagcattgct gatattttgg
     4561 aaatgacagt agaggatgct gtagaattct tcaagcacat tccaaagatt catcgcaaac
     4621 tgcaaacgat tgttgatgtt ggcttaggtt atgtgacgat ggggcaacca gcaacgacat
     4681 tgtccggtgg tgaggcacaa cggatgaaac ttgccagtga attgcacaaa atctctaatg
     4741 gaaagaattt ctatatacta gatgaaccaa cgacaggact tcatagcgat gacatcgccc
     4801 gcttgttgca tgtattacaa agattagtag atgctggtaa cacagtttta gtgattgaac
     4861 acaatctaga tgtaatcaaa acagcagatt atatcattga tttaggacca gaaggtggag
     4921 aaggtggagg aacgatcctt gcgactggaa caccagaaga aatcattaac gtaaaagaaa
     4981 gttatacagg tcactatttg aaaaaaataa tggtataaag aggatagata gctgagatag
     5041 ttaaaaaaac ataaaaaaat gaatttttgc ttgttttatt aatatcagtg ttctagaatc
     5101 attacatggt agttctttct acactgattg gtatagataa aaacttccta taagttcaat
     5161 ccgttcaaaa aagaattgag caatcggagg agtttccttt gattgctttt ttctttatgg
     5221 tatgatattc cttgtgtagt tggaaaagaa aaacaagaga ttatgagtaa gtgagagata
     5281 atgatgaaca gtattgaaaa tgaagaattt atccctatat tattaggaag cgacatgaac
     5341 gtttatggta tggcacgttc ttttaatgaa gcatacggca aaatctgcca agcatatgct
     5401 tcggatcagt tagcccctac ccgctacagt aaaattgtca atgtagaagt tattccagga
     5461 tttgataaag accctgtatt tatcgagacg atgcttcgtt tggcaaaaga aagatattct
     5521 gataagagta aaaaatatct tttgattgct tgtggtgatg gatatgcaga attgatctcc
     5581 caacataaac aagagctgtc agaatatttt atctgtccat atattgatta ttctttattt
     5641 gaaaggctga tcaataaagt aagcttttat gaagtttgtg aagaatatga tcttccgtat
     5701 cctaaaacat tgatcgtcag agaagaaatg ttagtaaatg gacatttgga acaggaattg
     5761 ccttttgaat tcccagttgc tttgaaacct gcaaacagtg tggagtatct ttctgtccaa
     5821 tttgaaggaa gaaaaaaagc ttttatttta gagactagag aagagttcga tttaatttta
     5881 ggccggatat acgaagccgg ttataaaagt gaaatgattg tacaagattt tatcccgggt
     5941 gatgatagta atatgcgtgt gctaaatgcg tatgtagatg aagatcatca agttcggatg
     6001 atgtgccttg gtcatccact attagaagat cctacaccag catcgattgg taattacgtt
     6061 gtgatcatgc cagattataa cgaaaaaatc tatcaaacca ttaaggcatt tttggaaaaa
     6121 atcgaatata caggctttgc caatttcgat atgaagtatg atcctagaga tggagaatat
     6181 aaattatttg aaatcaattt gagacaaggt cgtagcagtt tctttgtgac attgaacggc
     6241 ttgaatctgg ctcgttttgt cacagaagat cgagtgttca acaaaccgtt tgttgaaacg
     6301 acctatggaa cgaatcaatc agataaagca agattatgga tgggtgtgcc caaaaaaatc
     6361 tttttagaat atgctcggga aaatgaagac aaaaaactag ctgagcaaat gatcaaagaa
     6421 aatcgttacg gtactactgt attttatgaa aaagaccgat caatcaagcg ctggttgttg
     6481 atgaaatata tgttccataa ctatattcca agattcaaaa agtattttca tgtgaaagaa
     6541 ggctagttca tggagaattt tttcagtatt ttaggcggaa tgggcacgat ggcgacagaa
     6601 agttttgttc gcttgatcaa ccaccgaacg aaagctacaa aagatcaaga atatttgaat
     6661 tatgtcttat tcaatcatgc aacagttcct gatcgtacag cttatatttt agatcgatca
     6721 gaagaaaatc caatgccgtt tctgctggat gatattgaga aacaaaattt attgcgacca
     6781 aacttcattg tgttgacttg taacaccgca cactactttt ttgaagagtt acaagcggca
     6841 acggatattc ctatcttgca tatgccaaga gaagcagcaa atgaacttgt ccgtcagcat
     6901 acgacaggga gagtagcgat tctaggaaca gaaggaagta tgaaagctgg aatctatgaa
     6961 cgcgaagtca aaaatcttgg tttcgaaaca gtgattcctg atactgcgct tcaagaaaaa
     7021 atcaattacc tgatctatca tgagatcaag gaatcggatt atttgaatca agagctctat
     7081 tacgaaatct tagaggaagc ggtagagcgc ctaaactgtg aaaaagtcat cctaggttgc
     7141 acagaattat ctttgatgca tgaatttgct gaagataatc attacccagt gatcgatgct
     7201 caatctattt tagcggatcg aacgatcgaa cgtgcattag ctgaaagaag cgaggcattg
     7261 gatacagcat cggaaaagta aaaaaattgg atttggggca aatatcctaa aagatatttg
     7321 gtccaaatct ttttgtaaaa gaaaaagaaa actggtaatg atacgcccgg gatgatataa
     7381 ttttattatg cacaatgtga gaggggttcg taaatatgac agataatttg caattagtca
     7441 tcataacagg tatgagtggt gcagggaaaa cagtagctat ccaaagtttt gaagatatgg
     7501 gctatttttg tatcgataat atgccgcctc gattgattcc aaaattctgg gaattgatca
     7561 aagaatcagg taaagtcaca aaaatggctt taggagtgga tttgcgttct cgttccttct
     7621 ttgaagaaat ccaaaacatg ctgatcgaaa tcgaaaatac gaacttcatc gacacttcta
     7681 tcttattttt agatgcttca gacgaagaac tagtggctcg ctacaaagaa acaagacgag
     7741 cacatccaat ggcgatggac ggtttgatca ctgaagggat acgtaaagaa cgggcgatac
     7801 tagacgactt gaaagcccaa gcttccgtga tcattgatac gacaacattg acaccacgac
     7861 aattacgtga aaaaatcaat gagtcattca aatcaaaggg agaccaagga tttcggatcg
     7921 aatttgtatc atttggtttt aaatacgggt tgccgatcga ttcggatatt gtgatggatg
     7981 tgcgtttttt gcccaaccct cattatatcc cggaactaag accattgacc ggaaaagatc
     8041 agccagtcta tgattatgtg atgtcctttg atgaaacgca atcattctac cataaatttt
     8101 tggatcttct ggaaactgta ttgcctggat acattaaaga aggaaaaagc agcttgacag
     8161 ttgcaatcgg ctgtacaggc ggtcaacacc gttctgtggc attgactgaa cgtttggcaa
     8221 atgctttagg gaaaaactat aaagtcaatg tgacacatcg ggacaaggat aaaaggaaag
     8281 agacggtgaa ccggtcgtga aaatgaaaac ctaccggata agaaaaccaa agatcgtagt
     8341 ggtcggcggt ggcactggtc tgccagttat cctgaaaagt ttacgaaatc aaggagcaga
     8401 tatcacagca gtagtgactg ttgcagatga tggtggaagc agcggtgcaa tcagagaatc
     8461 gatcgctatg gctcctcctg gtgacttgag aaatgtctta gtggcgttat cggatatgcc
     8521 acaattctat gaagatattt tccaataccg tttcaaaaag gaagatcagt ttttagccaa
     8581 tcatacgatc ggtaatctga tcattgccgc agtttccgag atgcggggaa gcacttatga
     8641 ggcgattcaa ttattagcaa aaatgatgca tgtagatgga catgtttatc cttcttcaga
     8701 aactcctttg accttgcatg ctgtttttaa agacggaacg actgcagtag gagaatctaa
     8761 aatcgcgatc aaccgtaaaa ccattgaccg tgtgtatgtc tgcaacacaa atgataaaag
     8821 tgaagccaaa gcggctcgaa aagtcgtttc agcaattatg gacgccgaca tggtcgtttt
     8881 gggacccgga agtttattca ccagtatcct tccaaattta gtgataccag aaatcggcga
     8941 agcaatgctc aagacaactg ctgaaaccgt ctatatctgt aatatcatga cccaaaaagg
     9001 ggaaacggaa catttcacag atgccgatca tatacgtgtt ttacacaagc atttgaatca
     9061 gccttttatc gatacagtat tggtcaatac ggaaaaagtg cctgaagatt atatggaccc
     9121 agagatctat gatgagtatc tagtacaagt cgcacacgac ttcaacggat tgagaaatga
     9181 aggatgtcga gtgatttcca ccgacttttt agaattgaaa gatggtggtg tcttccacga
     9241 tggagacaaa gtagtagaag aattgttccg tttagttttc ggttcaaaat attaagcaat
     9301 ttattgttgg agaaaggagg agaataggat gtcatttgct gcagatgtga agaaagaact
     9361 aactggtttg gaagttcatc gagaacatgc gaaagcggaa ttagctgctt tgctgcgaat
     9421 g
//
LOCUS       DACEOE010000098         9028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEOE010000098 DACEOE010000000
VERSION     DACEOE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.100"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            248..547
                     /locus_tag="IW774_001884"
     CDS             248..547
                     /locus_tag="IW774_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154229.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(644..1078)
                     /locus_tag="IW774_001885"
     CDS             complement(644..1078)
                     /locus_tag="IW774_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1154230.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1310..2020)
                     /locus_tag="IW774_001886"
     CDS             complement(1310..2020)
                     /locus_tag="IW774_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1154231.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2010..2870)
                     /locus_tag="IW774_001887"
     CDS             complement(2010..2870)
                     /locus_tag="IW774_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1154232.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2875..3513)
                     /locus_tag="IW774_001888"
     CDS             complement(2875..3513)
                     /locus_tag="IW774_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1154233.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3528..3827)
                     /locus_tag="IW774_001889"
     CDS             complement(3528..3827)
                     /locus_tag="IW774_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1154234.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3870..5354)
                     /locus_tag="IW774_001890"
     CDS             complement(3870..5354)
                     /locus_tag="IW774_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1154235.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKATCLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5374..5835)
                     /locus_tag="IW774_001891"
     CDS             complement(5374..5835)
                     /locus_tag="IW774_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1154236.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5853..6920)
                     /gene="ulaG"
                     /locus_tag="IW774_001892"
     CDS             complement(5853..6920)
                     /gene="ulaG"
                     /locus_tag="IW774_001892"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1154237.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7138..7908
                     /locus_tag="IW774_001893"
     CDS             7138..7908
                     /locus_tag="IW774_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1154238.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8181..9008)
                     /locus_tag="IW774_001894"
     CDS             complement(8181..9008)
                     /locus_tag="IW774_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1154239.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2757 a         1878 c         1423 g         2970 t
ORIGIN      
        1 atcacacatg aaaagcgacg tacaaattgc taagattata gaattagaag aagaagacgc
       61 agtgttgaca attgaaaaaa aataaatgtt tgtataccat atgattattt caatatgtat
      121 cctggaattg gagatctcgt agagctatat caagatgaat caaatatact tgtcattcat
      181 catgaaatat agagacttat aaaattgttt atttttataa tgtatcagta tagaaaggaa
      241 ataaaacatg gattatactg ttgaatttga cagtgaacta cttaagcaat ttccacaaga
      301 agaacatttc atgtataaag aagcaatttc ccacaaagtg acagagtatt tggagcataa
      361 tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa ggaatatacg agatgaaagt
      421 caaaattaat aaaaaaaatt atcgcatagc atttgcttta aaaaaaaatc atgtgttcat
      481 tttttatatt agttcgaatt tgcaaaaaat tgtatttgat aaagaggtga gtaaaaaaat
      541 aaaatagctg agcattggag gatagattga gtgatactaa gtctatcctc ttgtttttct
      601 aaagaactaa gctagaattt tttcttttgt acgttattat atgttatttg tcattaccaa
      661 aaagcacctt ttttcttcta gtatctatga cataagaaat accacccatt tcctttactt
      721 gaacaaatcc tactgattca taaaattttt ttgttttctc agtatcatct gtcaataatt
      781 cgatttggac tggtccataa ctagtgatct tctccaaagc ttcattcaaa agcactttgc
      841 ctattccttg gcgctgatac ctcggatgaa tgaggatatc ttgaataaat gcgatcgtcg
      901 caaaatcagt gatccaacga acgattccga taatcttccc ttccccatta cgctttatca
      961 gagattcaga ttgttcaaat gctctagcta accttgcagt gtcttttgtg taattcgtcc
     1021 aaccaacgct cttatataat tcagaaatct catcgatcat aagggaatca atattcattt
     1081 ttttcttcct ttcttataca attctccctc taataataca gttctttgac tatttaatct
     1141 atcacatata actctcctgt aaagattctc tcgattcctt aaaattcctc tttcctttta
     1201 gaatgcatac tttttgataa ataaaaaggt tgtgataaat tttcttcaca acctctgttt
     1261 ctctctatca agtgttatct tttaattttc tacccttacc ctagcatctc tattgcccgt
     1321 agtatgcata ctttccatgt ttgcgaaaat aatgtttatc tagtaaatat tgcgggattt
     1381 cacatatatt ggggttgatc agctcgtttt ctttggccat cagacaaatt tcatctaaga
     1441 tcaaactgtt ttctactgct ttcataggtg aatcccccca tgtgaacggt ccatgtccat
     1501 agacaagtac tccagggact tcgtttggat ctaattttct ttccttaaac gtctcgacaa
     1561 tcacattccc tgtatgaact tcgtaagctt ctcttacttc ttctttcgtt aattggcgtg
     1621 tacaaggaac cttaccataa aaagcatcag catgagtcgt gccataagct ggcaaatcgc
     1681 gtcctgcttg cgcccacatg acggagtgcg tggaatgagt atgagtaata gcagttatat
     1741 cttcaaaagt ttgatacaaa acaacatgtg tggggagatc tgaagatggt tttaagctat
     1801 cttcttctat tatatttccc gacaaatctg ttaccaccat ttgatctgct tgcatgcatt
     1861 catatctgac accgcttggc ttaatgacga tgactcctag ctcacggtcc acttcactca
     1921 cattgcccca agtcaatttg actaatccgt atcgcggcaa tgccagattt gcttcaagga
     1981 cacgttgctt cattgctgca attttttctt cacaagacat catagccagc ctccttcaat
     2041 ttagaactta agtagtcttt tgcctgctgg atttctcttc gaaagtcttg gcttttttca
     2101 ctccacattt caatcagaaa tgttccatca taatctagac gtttcaaatg tcttaataat
     2161 ccaagaaaat caacacaacc gctaccaaaa ggaacatcac gaaactggcc tgaatattct
     2221 tttgttacag ggtaagtatc ttttaagtgg atagcagtga tataatcgat ccctttctca
     2281 agttctcgag ccggattatt ttccggccat gcggacaaat tgcctaggtc tggatatact
     2341 tgtagaaaag gtgaatgaat ctgttctttg atttctaaaa attttgaaat ggagttcata
     2401 aatggatcat ccatgatttc cacggaaaga atgatgccat atttagaagc ttcctttacg
     2461 ctttctttta ttccttcgat aaagtcttct ctggaattca ttgatttctc ttcataataa
     2521 acatcgtatc cagctatttg gattactttg acgcttagat tatgcgccaa atggattgct
     2581 tttttcatta gttgtttcgc tgcttttttc ttttctggat cagcagaacc aaacggaaat
     2641 cgccgatgcg cacttaaaca aatcgaatgg atacgtacac cggagctgaa catctcttct
     2701 ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg ctaaccgctc atccgtctca
     2761 tcaatcgaca tctcgataaa atcgaagtcc atttctttta caagggaaaa acgttctttc
     2821 catgaaatat ctttgggtaa cgctttttca taaatcccaa tttgagccat ttccttaccc
     2881 ccagatacga atgatctcat ctttgaagtt ttgtgcggcc ttcatcggat catttgctgc
     2941 tgtaatacca cgaccagtga taaaggtata cacatcgatc ccttcaaata agtgtaatgt
     3001 gtgtggattt aatccaccag ttacagaaac attaaagcct agttcaatca gttttttgat
     3061 cttcgataaa tctttttcac cccaagtttc accagataac agcgcatcac ggctttgatg
     3121 gtagattacc tgccggatac cgatgttatg ccaatccatg gcttgttcaa aagtccaatt
     3181 gccgtagagt tctacttgca attcacctat ttctttttgt gctgcctcca tagttggaat
     3241 cgttgcacaa caaatgactg tcatccagtc tgctcctgct tttgcaacat tactcgccac
     3301 tgttcctcca gcgtctgcac atttcgtatc agcgactagt tttttatctg ggaacatact
     3361 gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa agaattgtgc ccacttcaat
     3421 aatatctact acatcaccaa ctttcataac atctcctaat gcatgttcta agtcattgtg
     3481 atctaacgca atttgcaaat tcggcctgct catcttagtt ccttccttta cttgtttttt
     3541 tggtaccact ttgtttcagc aaatttttca ctgatttctt tgactgacat cacatttttg
     3601 ataccgataa ctgtaactcc ttttttctct gcctgagtaa acatattcaa aaaattgact
     3661 ggcgtaaaaa ctacatcgta ttggccagct gaactttttc cctctgaaat ggcacaatga
     3721 tggattttgg taattggtac gtttagttct ttcattgctt tttccacact tctcatcatc
     3781 atcaagcttg ttcctgatcc atttgcacac gatactaaaa ttctcatctt aatccctcca
     3841 ttatgagtgt tgtctaatca gattttcttt tatactctct gcttttctgc atatgtgtca
     3901 tagtcttcta caatcaaaaa ataaccatca ggatttttcc gatattgtaa ctgcggaata
     3961 gccaataaac caatgacaac tacggcaatt ccagcataac ctaggacttt catgattact
     4021 gtaaacaatg gccaagctgt tgcccagtcg aacatcccga tataaccacc gtatgttgac
     4081 atcccaatcc atgtagcgaa taatgctgaa ccgcctactt gtatgattcc tgaaagaaac
     4141 gggaacaggc aagtggcttt gaatccacct cggttattgg cataaactgc tataacggca
     4201 ttatcaaaga acaacggaat gaaaccggct atgacgatcg ttggtgattt caataaaatc
     4261 agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca agaacccaat cgttaccgca
     4321 tttggcgaac caaagccaaa agtagcagct acgtcaattc ctggaacagc tcctggtaaa
     4381 atcgtgtttg agattccttg gaaagattga gttagttcat cgacgaatgt ccgaacacct
     4441 aattgcaaga tcgccagata taccgcaaaa tttaaagaag tcgtcataat gtagaataag
     4501 aaactctgcc cttcttgcat gaattgcgct tgaatcaaat aatctttgcc tagtacaatc
     4561 agaatgatcc caaagaaaaa tagcatgagt aacgaagtag caaccatatt ctcattgaaa
     4621 atcgacaaga atcctggtaa tttgatatct tcaatttttt tgtcttgctt gattgatcta
     4681 tttttctttt catcccgctt tttcatccac tcagctaatt ttgcaaatat aaagattccg
     4741 aacatttgct gatgtgccac tgcaaatccg gcaccttctg tcaattcctg cgtgattcct
     4801 actgttaagt tagaagctac tgtccaataa caacctaaaa tcagtcccat aaaaatcaat
     4861 gcttgtatcc tacctagatc aggaaagcaa aacagtaaaa tccaaaaagc ggtagctgct
     4921 tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca gtttcgtata tttctgaaaa
     4981 cggactaata agatatttaa aacaaatgca atcagtaata aaatcatcac atctccaaag
     5041 gtacggccaa atgtctccat taatccagca tccacagcat tttgtccaaa ataaggatct
     5101 gtaaccatgg catccaaatt gaaccgttcc tttagtccaa ccaagattgg ccgaaaatta
     5161 ttgaccaaac caccagagcc aacagtcaaa ataaagtaac cgaccgttgc ctttaagaat
     5221 ccagcgatgc attcatgaag cgggcgtttc agcaaaatat agcctaataa cacgataaaa
     5281 ccaatcaggt atgctggctg cgtcaagata tttgttgcaa aatacgtcca aatgcttaac
     5341 aaaaattccc ccatcttttt tctcctctat caattaatta tattctgcca tcacaacttg
     5401 gtaatcatcg atggagttcg tagagatgag acgctcaacc aatccatctg tcattaataa
     5461 ttcggataat ttttggatat tttctagatg ttcttctgga tcttttgctg cgagtgtaaa
     5521 aaacaaacta gccgttttct cctcattctc cccttcactt tcaaaacgga ttggctgttt
     5581 caacttggta aatgaaatag ctgttccaaa aacaccatca cttttttctg aagaatgcgg
     5641 cattgctact tgaggtgcga tcacaatata aggaccgtat ttttctacac aatggatgat
     5701 ctcctccaca tacgtttcat caataatttc cttatcgatc aatacaagac agctttcggc
     5761 gatagcttca cgccaatccg tgatttcttt atctgggtaa tgaatcaatt gatggtcaaa
     5821 aaagtatttc agcaagaaac tccccttctt tcttataaaa acgacggaaa cggtgtatca
     5881 ttttcaatcg agaagacatc ttcaaatcct cgataaaaat taaactcaag atcatctttg
     5941 tttgtcggat agatgaattt cccgcctact tgccagatat aaggtttaaa tccatacttc
     6001 aggcgatcct ttttgaattt ccaaatttca gtgatctctt ttggatcagc cataaaatta
     6061 gaccaaatat cgtagtggac aggaatcact acttttgcat tcaatgactc cgccatgcgc
     6121 agcatatcaa cagaagtaac tttgtctgtt atccctcgtg ggttttcacc ataggcccct
     6181 aaacagacat cgattttgtg ttcattacca tgtttggcaa ataaattgga gtaatgtgaa
     6241 tctcctgcat gataaagatt ccctcctgat gtttcgaata aatagtttac tgcaatttga
     6301 tccatctcct gaggcatttt tccttttaat acctcttctg gatcttcaca agtgaccaac
     6361 gctgtccgat caaacgcttc taaagctact acattgatgt ctttgatttt gactgcatca
     6421 cctggcttta ctgtaatcgt tttttcttca ggaattcccc atttttgcca cgtgcgaacc
     6481 acttcttttg gcccaatgaa tgttgcttca ggacaatttt gatgaactgc tgcggctgta
     6541 tggatatcta gatgatcgga atggatatgt gtaacgatta aagcgtctac attttttatc
     6601 gcaaaaggat cgattacgaa aggctgagta cgtaaattag gctgcatatt ttgaacgcca
     6661 ctcatacgca tcatctgatg gccttttttc atcatgccat tcccatgact acgttttccc
     6721 gtcccacacc atagatcaca taaaatattc gttccttcat gacttttcag ccaaattccc
     6781 gtacagccca gccaccacat agcaaaagtt ccaggatcca ctacttctgt ttcgatttct
     6841 tcattcaagt atgtgcccca ctcaggaaat gtttctacta accacttttc ttttgttact
     6901 tcattgacag ttttactcat ttgcttgctc cccccatatc gttgttccaa cttacaagta
     6961 aatattaatg tgtatcagca tccatgaaaa cccttttata tgaccgtttg ttttttatat
     7021 gattttcttt cagtaatcaa tgattgtttc agtataataa agacagatat gaatgtttaa
     7081 atgaacaact agtcaaataa atccatggat caagatgtcc ctcaggaggc aaaagtcatg
     7141 aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa ttttggaact gcttcagcag
     7201 actgaacaac tatcaacttt cgaattagct gaatcccttg atgtatctat tagtacgata
     7261 cgaagagatt taaatttatt agaagaaaaa aacgagatca tacgcaatta cggttattgt
     7321 tcttataatt acaaaaacca gactgatttt gatcaatctg gtcctgaaag gattaaacaa
     7381 gcgattgcca gagaagctag taaatacatt aacgattatg atacgctgtt tatcaattct
     7441 agctcgactg cgttgaaaac actggatttt cttcaatcta accatttgac agtcattaca
     7501 aacaacgtga aaatcaatta ctctccgcac aaagataatt gtagttatat tttaactggt
     7561 ggagagatgc gctttccaaa agaagcacta gtgggcgaca tcgcattaaa tacgatttct
     7621 tcagtcaatg cagacatttg tatcattggc tgtagcggag tcgatgtagt acatggggtc
     7681 acaacaaaga tattaaatga atcaacgatc aacgaaatga tggtcaacaa gacaatcaaa
     7741 tgtaagattt tagtcgctga tcatcgaaaa atcggactga catcaaagtt caagattgct
     7801 gatatttcag tctttgatta tttgatcact gataaatttt gttctgaaaa agtattagag
     7861 gagatcagaa atacggggat aaaaaccatt caagtagatt cattttaaga accaggacgt
     7921 aaatatgtaa ataggattat caaaaaaatg aataacaaat ttatcgataa atgaaaaaca
     7981 cgctacgctc aaattgataa aggaatccca agatgtatcc atccaatcga taccatcata
     8041 atctattgct tcttttataa ccgttatatg ttgcctaaat aggaatcaaa caatatcgaa
     8101 aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa taaacggtat tcaaatcaga
     8161 acactgattt cacaaccact ttatttcagg tcgttttcaa tcacctttac cgcgtaatca
     8221 tttccgttga aacgcgctat tttttttgcg tccatatatc tttctaaata atcatcttct
     8281 cctaattgct tatggcaaga agcaatttca taaagcaagt attccaatgc ataagtcgat
     8341 ttatgtttct tattatactc aatacccttc tcacataatt ggattgcttt atcataatcc
     8401 tcgattttac ggtaataggt ggcgatatca taatatacaa tgcagaccat cagattatca
     8461 aaatcagtct cactcaatat tttaagtgta cgctccacat aaactttcgc aaaatctaat
     8521 tctccttttg acaagtagat cattgccacg tttgcagctg cttgtaagtg atggatatct
     8581 gtttcggtcg tttgttccaa cacttgatta aaataaaata atgctgcatc tggttttttc
     8641 gcataaagat gatcaagcag tccttgataa aataaaattt gatttaaaag atcagaatta
     8701 cttacttttt cctgatcgat cgtttgtaaa attttataag attcttggta ttttgataca
     8761 gaattcaatg attgaatctc atttattttt ttcctgattt tgtcttcttc tgtttctact
     8821 aatatatcgg agatctcgac attcagcttt ttgcaaagcg cacttaagat attgatgcct
     8881 ggcgacatat tcttgttttc gatattgctg acagttgctt gttcacaaat gccttctgct
     8941 aattgtactt gtgttaaccc ctttttcttt cggtattctt ttagtaatgc tccatctact
     9001 ttaatcaaaa caatttcctt ccctttaa
//
LOCUS       DACEOE010000099         8919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEOE010000099 DACEOE010000000
VERSION     DACEOE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.101"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..257
                     /locus_tag="IW774_001895"
     CDS             <1..257
                     /locus_tag="IW774_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154240.1"
                     /translation="LSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHR
                     QFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            271..960
                     /locus_tag="IW774_001896"
     CDS             271..960
                     /locus_tag="IW774_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154241.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1085..3436
                     /locus_tag="IW774_001897"
     CDS             1085..3436
                     /locus_tag="IW774_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1154242.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3516..3911
                     /locus_tag="IW774_001898"
     CDS             3516..3911
                     /locus_tag="IW774_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154243.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4236..4853
                     /locus_tag="IW774_001899"
     CDS             4236..4853
                     /locus_tag="IW774_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1154244.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            4894..5193
                     /locus_tag="IW774_001900"
     CDS             4894..5193
                     /locus_tag="IW774_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154245.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5227..5394
                     /locus_tag="IW774_001901"
     CDS             5227..5394
                     /locus_tag="IW774_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1154246.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5439..6002)
                     /locus_tag="IW774_001902"
     CDS             complement(5439..6002)
                     /locus_tag="IW774_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1154247.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6030..6710)
                     /locus_tag="IW774_001903"
                     /pseudo
     CDS             complement(6030..6710)
                     /locus_tag="IW774_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(6784..7065)
                     /locus_tag="IW774_001904"
                     /pseudo
     CDS             complement(6784..7065)
                     /locus_tag="IW774_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7527..8909
                     /locus_tag="IW774_001905"
                     /pseudo
     CDS             7527..8909
                     /locus_tag="IW774_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3050 a         1357 c         1710 g         2802 t
ORIGIN      
        1 atttatccgg ggaacagtta gaagaaagta ttccaggaat tagtgactca gataaaattg
       61 ttgtcttgtc tgctggtggg caattaatgg gagatggcaa atccgaatta gttagttata
      121 aacaaaatca cagacagttt attgatgtta atactgattc caacgctatt tcgattaagg
      181 atctaaaaga gcttttaaat agtaaaagaa atgaattaaa gcagataaaa gaaactaatt
      241 cagtggaatt agagtaaagg aagattcatt atgaaaaaga tttttggtgt ttctgttggg
      301 ctacttatga tgttaacatt tggaaatgca gtatatgctg ctgagaaaga agttatttca
      361 gatccagagc agtacgcctc acgatacttt gctgattttg aagaagaaga taaagtactt
      421 gcattgccag aaggtggttt tttaaaaggg gaagctaaaa ttgtatctat ggataatcca
      481 gatataattc aaggttcata taattcagaa actgatcctg cttcagtttc agtgacagaa
      541 gcaaaaaaaa ttgttgctga aaaattcaaa aatgatattg aaactactcc aattatgcct
      601 cgaggagcat ttccacccga tgttggtact agcggacacc ttcttgtact agcaggagga
      661 ggaagctata aatcacagcc atttagtggt gcgggatggc gttttggagg tatgggtgta
      721 aaaccagctg atggtacagg caacttttta cgttggaaat cctacattga cgatggaaga
      781 gtaggaaatg taaatcaagc caacgcaact aaatctggtt ctattcaagg aatggcattg
      841 tatgctcgtg aaggattcaa atactttgca ccaaacgggc ctggttggac aatgaatata
      901 ggtatttatt acacttataa tcctgttgca ggtacgtatt atgttgttca aaatatttaa
      961 taaaacagca caaagagatt tctttgtgct gttttattat ttttagttgt tgacaaacac
     1021 taaaatataa aatataatca acacataaac gaacgtatgt tcggcaagaa agcaggggat
     1081 ttttatggat gagatggttt taagtactca gaaatggcta aacaagaaat acagtaatgt
     1141 tacagggttt gataaggttc cagaaaatgg gcgaacaggg tggcctacaa tctatggact
     1201 tattgaagga ctacaagtag aacttggtat tactaatcta gtagctaatt ttggacctac
     1261 cactgaaaaa atgtacgata atcaggttac tcctaaatgg ggcaaaaatc tacctaaaaa
     1321 tattgtattt cttattcaag gtgccttttg gtgtaagggg attaatccag gtggcttcga
     1381 tggggtatat actccatatt tagatactgc tgtaaaagag ttgcagactg atgcagggtt
     1441 taatggaagc gctgtaactg gagttttgga tgcgaaatgg gccaaagcgt tatttgatat
     1501 gagtgctttt gttcttgttc ccggcggaga tagtaaaatt agaaaaatgc agcaatggtt
     1561 aaatgtaaat tatttagaat acacgggaat aatgccttgt gatggtattt atcaaagaaa
     1621 ttcaaatcaa gcattaattt ttgcattaca agctgaacta ggatattctc ccagtgaagc
     1681 aacagggtct tatggtaatg ggacaactag taaaacttat cctgtgtctg aaggtaactc
     1741 gggaaactat gtaagaataa ttcaatatgg attatatgtt aatggttatt ttgaagacgg
     1801 tacattcgat ggtattttca ctaaatatat gggtttagaa gtactggcat tccgaaaatt
     1861 tatggtattg cctgaatata ctgaaatagc agatgttgtt gtaataaaag gactattgtc
     1921 tagcgctgga gatattagaa gatctgcaga tggcgcagat acttctacgc aattaactag
     1981 atctcaaatc caaacattgg ttgataatga tataaaaata gttggtagat atcttactgg
     2041 tactgttgga attggaaaag atgaacgaaa taagtatcta actacagagg aattgaataa
     2101 tattttttct aaaaatttat ctgtgtttcc tatctatcaa gatggaggtg cagcattagc
     2161 ttattttact tatgatcgtg gcttatcaga tggtaagaag gctatagatg cagcaaaaaa
     2221 tctaaatata ccattaacaa cagttattta ttttgctgtt gatctagaca tgttagggga
     2281 agaaattctt gcatatgctg gagaatattt taagggggta tctgctgtaa tgtcatatag
     2341 cgggtatcaa acgggtgttt acggaacaag aaatgtttgt tctcaaataa tcaataacgg
     2401 gtatgcttcg tttgcatttg tttcagatat gtcgactggt tatagtggga atttaggttt
     2461 tcctatgcct agacaatggt cttttgatca atttgttgaa tttaccatag gaagtggcaa
     2521 cggagctgtt ggtatcgatt taatcgctac atcgggtaga gatagcggat ttaatgagtt
     2581 atcaaatgat actaataatg ataattacat tgccaaatac aatcagaagg ttataaatca
     2641 gatggtacgt gcgtatcaat atctatcaca agctggtctt gataatcctt ggaaccctca
     2701 tttgacattt tatagatatg taaattattc tggtttgtca tgggacatca tttcttctcc
     2761 tgtaacagag catgatagaa aaatttatga tgaatatagg aataaattaa ctaatagcga
     2821 gggcctgtat aattatttta tagatcctaa tactggttca ataataggtt tgcctcactt
     2881 aattgtgacg ttacaatcac agatgtttct agccgacaca atcaatgata gtgtttctga
     2941 ctttgcaggt tggttaggag atttaatgac ttgctggggt gaagttaaga aattaggaat
     3001 acaaatggag caaggtgtat ttgcattagt aggtacggct gcaggtggaa catttagtct
     3061 agaagattta taccaagatg ttgacgcggt aaatgtttat accttagcaa aaagaaattc
     3121 tgattcaaca gctttatcgg ctttaaaaac gtactattgt gataagggtg ctattgatag
     3181 atttaatcaa tttgttaaga acagatttgg ttcttcagat aaaatcgctt cagtaacaaa
     3241 tagtttatta gaaaatgacg aaacagttga tatcttaggt gtagggataa tttcattttt
     3301 cattgataaa atagttaata attcacttgg gacagatttt acttatcttg attttattgg
     3361 agatgagggg aaagttgcaa atggattttc tgaaaaaatt attcaatttc aagaaaatag
     3421 aagtaaactt aattaagaat cgtttactat ttaaacagtg tccttttcct ttttaaatat
     3481 atacgataaa ataagtatat gaattgtaaa aggacatgat gattatggaa gaaatagaat
     3541 ggaataatct gactcaaaat aataaaaata attctggatg tggaattatc tttatgtttg
     3601 ttatagtttt gggaatagta gtacttttaa taactgtcaa gatagtagat tactttgcga
     3661 atactcctga agaacaaata cagagaaaac ttcctgagtt tgaaagagta ccaccttatg
     3721 atccaagaga taattcattt caagtaaaaa aattaaaaga gtctccaaat gaaatgtggt
     3781 ttgaagttac agttgaatca agcgacttca aagaaaaaaa gaaagaatac aattatattg
     3841 taaaggcaaa agtatcaaaa agatttattt ttaaccctaa attagaaata tatagtgttg
     3901 taatagaata atagtgttaa aaaaattgta aaagttaaag aagtcaaatt ctgatgtgaa
     3961 aaataaaata taccatggaa gacataaaag aaaaactgga taactagttt atttaatggc
     4021 agaccgttaa ggcctgccat ttttatgtat tttagcggaa taaatataat catgcgggag
     4081 tgtaaactta ctgtttcaaa aagtatactt tttgacatgc cttgattttc tttgtttatg
     4141 tatggcataa taaccacata atacaatttg aaacgtttct gatttaatct gattgttttg
     4201 aaacaatttt agtgaaaaaa gggtagataa aaacgatgag aaaatttttt tatatccggg
     4261 tcagtagtta tgatcaatcg atcgaacgac aactgatttc cgcgaaggaa ttaggaatcc
     4321 ctgaagaata tatatttgta gaaaaagcca gcggcaaaga ttttaaacgc cctgaatatc
     4381 aattaatgaa acggatgttt cgaaaaggag atttactcta tctacaatca ttagatagac
     4441 taggcagaaa taaacaaatg attttagaag aatggcgaga attaactaag gaaaagcaga
     4501 ttgatatttc tgtgttagac atgcctctgt tagatactgc tagttacaag caactagatg
     4561 ggttagagac gcttatctct gacttaattc tgcaattact cagctatatg gccgaagacg
     4621 agcggaaacg aattaatagc cggcaaagag aaggcgtaga agctgcgaaa caaaagggtg
     4681 taaaattcgg cagaaagaaa attgctttac ctcctgaatt tcctgcgatc tattctgact
     4741 ggaaagcagg aaagataact gcagtctatg ctatggatca attgggcatg aaacgaaata
     4801 ccttttatcg acgggtgaaa gaatatgaac ataccttgca ccacttttct tgatagatat
     4861 aacagctatt agacgggaag ggagagtttg aaaatggact taacgaatca aagggtcgtt
     4921 tttactggaa cgctgcagca aatgacacga acccaagcca tcggtttggt tcactcttta
     4981 aacggccatt gtcaatcaag tgttacttca aaaacaaatt tcctggtttg tggtcagtat
     5041 tctaagtcgt tgtttgatga ttctctctat acaaaaaagg aacaaaccgc gcgggacttg
     5101 attgcgttag gtcatgagat taccatttta tcagaagtcg aattttatgc gttaattagt
     5161 caacagttga aagaatggca acggtattta taatcgttag taaaatttac aaggaggaaa
     5221 atttttatga agcgaaaatc ttatgatcgt tggggcattg aaataactag ttttaatcga
     5281 aatattgctt ttcacgtgga gtgtgactgt ggggaattag caaaaagtgt ttatcaaaaa
     5341 aattactttt actgtcctaa atgccatcgc cgatacgggg tacatggaac atagaaacga
     5401 aatagtgcgg tagaaaagcg aatgaatgta gtatatttct acaaatcatt cgctttcttt
     5461 attttataaa tagttggtcg agagatcccg ttttcttttg caatctctgc aactggaatg
     5521 ccttgcttta acatgtccac cacggtttta aaaactaagc gtttttgcgg atctgttgag
     5581 ttaatagaat actcgattgg tcgtccctta tatcggccgg ctttttgagc caataatact
     5641 ccttgccgct gacgttcacg aattttctca cgctcgtttt gtgcaatata acttagcagt
     5701 gataaaaaca tatcgaacat agcggtatcc aggagttcat ttccagtatt aaaatctaaa
     5761 aactgtgcat ctaatatttt taacgctact tttttctgtt tcataaaagc aactgtattt
     5821 tttatatctt cataatctct tcctaaacga tctagtgatg taacgatgac gcaatcacct
     5881 tcacggatat aatttagtaa tttttgaaac tcaggacgct cagtatcttt accactggct
     5941 ttttcttgga ataatttatc aatgttaagt ccttcggttc tgttgcgaag tttgaaaatc
     6001 aaacgcgccc tctctgaact ccaaatatct taggctgtta ttcccattaa taccttgatt
     6061 tcagtagaca ccgaaaagcc gaagagcgtt ccatttcttc ggttcttttt atatattcct
     6121 cgaatggtct ccatgccctt aatcgtggaa gaggctgtac ggagactttg ataaaattta
     6181 tttcgtcgtt taataggtcg atggtcttgt tctattaaat tgttaagata cttcacagtt
     6241 cggtgctctg tcttagtata taaacccaca ctctgtaact ttctaaaggc ggagccaaga
     6301 gaaggtgctt tatcggtcac aattgctttc ggctcaccaa actatttatg gagtcgtttt
     6361 aagaaagcat aggctgcttg cgtatcccgt ttctttcgta accagatatc taaggttaag
     6421 ccgtccgctt caattgcacg ataaagataa tgccaacgtc ccttaatttt gatataggtt
     6481 tcgtccattt tccatgaata gaaggattgt ctatttttct tcttccaaag ataatagagg
     6541 actttgctgt actcttatac ccaacgataa atcgtagtat gacaaacatt tattccacga
     6601 tcatataaca attcctgaac ttcacgatag cttagattgt aacgcaggta gtaaccaaca
     6661 gcgacaataa tgacgtcttt tttgaattgt ttgcctttaa aatgattcat tactctgtcc
     6721 tctctgtctt ttttctcaat tttacactaa aatagatttt ttggaaaact ttgcaacaga
     6781 accgaaatga gtatagtatg aattagtaat ttggttttca tctaagttta gctttgattt
     6841 actaaaattg atatctcgat acatgttcaa tacatctaat acaaatcgag aatcttcttt
     6901 cggaaatggc tcagaaagat cactccaaat ttcatcatca taatgatact cgtaaccttc
     6961 tctcaatatt tcgatttttc tctcataata agatctgttt tggtcctcaa atttagccaa
     7021 taactcaaat tgatttacta gtgacaatct ctgaaaactg tccattaaat gtttcttcct
     7081 atcttgttca ttaacaaaaa attataagcc cttacaacga atattatata ttaaatttaa
     7141 ttgtaggtga acacgaattt tataaatatt tatggtgtta atgacaaatg atatccgact
     7201 cctcttcata cgttctggac tatttgctaa tagattaaaa tttcagcatc tatttctgac
     7261 cataaataaa tgagctgccg aaaagctgcc aaatcagact tgaaaataaa ggatcaaaaa
     7321 cttttaagtt tctgaccccg taactcacac ttcaaaatct tacttaaaaa ggagtctgaa
     7381 agaaaaaaag agtattcaaa atttttttct aactagctac atcacatcta tgcattgaca
     7441 ctcaaagaac cagaataagt catcagaaag caagggggtg cggacataat cctggacgaa
     7501 aaaataaact taggaggaat gtccgtgtgt tttcccacaa agagcgtgta agggcaattc
     7561 agttattttt aaaatacgat tgctcttatg ctgcaaccat tcgagaatta gggtatccgt
     7621 cgattggtgc actacggaaa tggtataaag agtatttgga atccggagag ctccatcagg
     7681 agcaccgaaa aaaatcaaag tattctgaag aacaaaaacg aatagcggtg aatcattatt
     7741 ttgaatatgg tcaatgttat gcacgaacca ttcgcatgct tggttatcct aatagagagt
     7801 ccttgcggca gcggtgtgaa gaacttgcac caggagctag aaaactccga aaaagtgcgg
     7861 taaagttaac acaagatcaa aaagaagctg tgttgaaaaa attctataaa ccgcaaacta
     7921 ataggaaaaa tttagccgaa tctggaggaa ttagtagagt tactctatat caatggaaaa
     7981 acacggttct tggaaaggat tttccattgt gtatgacttt aaaaaatcag gaaaaacaaa
     8041 aagagctact tttaaaggaa gtagaagact tacaaaaaca agtacaccaa ttacagctcg
     8101 aaaaagcatt actcgaagga gcggctgaac tgctaaaaaa agaaaaaggc gtcaatcttc
     8161 tttgtctatc aaatcaagaa aaaacgatat tgattgatgc cttacgaaat cagttcacct
     8221 tgaaagagct tcttcaacag ctccagtttc ctaaaagcag ctatttttat caaaaacaag
     8281 cacttgagaa accagataag tattacaaag aacgtcaaat taattatcac gattttcaat
     8341 cataacttct gtgcgtatgg ttaccgtaga attcaccaag ctttaaaaaa tatgggtaaa
     8401 aagctttctg aaaaagttgt acgtagattg atgactgagg aaaatctatt tgttaaattt
     8461 tctagacgaa aaaagtattc ttcgtatgct ggggaaattt ctcctgcaca ccctaattta
     8521 ttaaatcgga attttaaagc aaagaagcca aatgaaaaat ggttaacaga tattactgaa
     8581 tttagaattc tcgcagggaa aatctactta tcaccacttg tcgattgtta cgatggtgct
     8641 attgttagtt ggactattgg aacaaagcca acttgcttca ctctatgtct aaaaagggtt
     8701 attcaccaga taattcagcg tgcgaaggtt tctttggacg attaaaaaac gagatgtttt
     8761 atcaacgtga ctggaagaat acaacaatca atcagtttat aaacaaattg gatgattata
     8821 ttcattggta taataatcaa cgaattaagt tatctttagg agggctaagc cctcttcaat
     8881 atcggaagaa gcaaagatat atttgttaaa aaggtccaa
//
LOCUS       DACEOE010000100         8747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEOE010000100 DACEOE010000000
VERSION     DACEOE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.102"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            276..458
                     /locus_tag="IW774_001906"
     CDS             276..458
                     /locus_tag="IW774_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154248.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            442..627
                     /locus_tag="IW774_001907"
     CDS             442..627
                     /locus_tag="IW774_001907"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ1154249.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            870..2390
                     /locus_tag="IW774_001908"
     CDS             870..2390
                     /locus_tag="IW774_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1154250.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(2577..3149)
                     /locus_tag="IW774_001909"
     CDS             complement(2577..3149)
                     /locus_tag="IW774_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1154251.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(3156..3818)
                     /gene="rpe"
                     /locus_tag="IW774_001910"
     CDS             complement(3156..3818)
                     /gene="rpe"
                     /locus_tag="IW774_001910"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1154252.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(3834..4349)
                     /locus_tag="IW774_001911"
     CDS             complement(3834..4349)
                     /locus_tag="IW774_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ1154253.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(4351..4635)
                     /locus_tag="IW774_001912"
     CDS             complement(4351..4635)
                     /locus_tag="IW774_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1154254.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(4669..5997)
                     /locus_tag="IW774_001913"
     CDS             complement(4669..5997)
                     /locus_tag="IW774_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1154255.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(6011..6466)
                     /locus_tag="IW774_001914"
     CDS             complement(6011..6466)
                     /locus_tag="IW774_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1154256.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(6469..8415)
                     /locus_tag="IW774_001915"
     CDS             complement(6469..8415)
                     /locus_tag="IW774_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1154257.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2917 a         1625 c         1372 g         2833 t
ORIGIN      
        1 aaagattcga ttaaattagt tgagtaaatg cttcgtacga acatagttga tttattcacc
       61 atacttccca ttacatcgta aaacgctata tttaagattt caaacaacta tattaatagg
      121 aagaaaactt catttttcct cttagttcta taaaaagtgt ggctaatttg aggcaaaata
      181 ttaaattaaa tgtaagcgtg tcataggata gtatctcgta cgccgattat ctttcttcta
      241 taattaaact atcaaatata gaaggagaaa aacgaatgaa taaaaaagat attgtaccag
      301 ttcaattttc aagtgcggct caaaaatcgc aaattgacac caagccaaaa ctagcttgca
      361 gaatcaaatc ggaagataag gaaatattta tttataatgg aatggataaa tatcttttgt
      421 ataccttgtt aaaagagttg aatgccaatg ctcattgatg caactgcatt gaaacatatt
      481 tttatcgttt gtggaaaaac agatcttcga aaaggaattg atggactagc agctttcgtt
      541 atagaagaat atgaccttga tgtttacgat caagcgcttt tcttattttg cggtacgaag
      601 aaagatcgct tcaaggctct ttactaggat caaaatggat ttgtattaat gtacaaaaga
      661 tttgaaagtg ggtaccttca atggccgcgt tccagaaaag aagtcttact gactggcctt
      721 tcaatcgaag aaaaatgtac gctaaaagaa acgaaaaaag gtgcgatata ttagacgaaa
      781 accttgttaa atcaaggatt ttgtctgttt ttcgttgtca taaaatggta taatagatgt
      841 aagaaaatcg ctagaatggt ggtgtaaaaa tgacaaaaat ggaatctgaa cttcttgtac
      901 aaatcaagca attaacacag ttgcttgaaa tccagcaaca ggaaaatact cttcttagag
      961 aacaaattgc tgaaatgaat cgacgcttgt ttggacgaaa aaaagaaaca ccacccgtag
     1021 atgggcaaat cgatttatta gacgactcaa cttttaatga accagagcac actggacaag
     1081 aaagccagga acccattacg gtatcttctt ttaaacgcag aaaacgcaag ggactcaaag
     1141 cactttcatt agaaggtctg cctgaagttc ctattcatta tgaattaaaa ggtgccgatt
     1201 gtttgtgtga agactgtggt catactttac atgatatcgg tgcgacaagg cttagagaag
     1261 agccattgta tgttcctgcc catatagaaa aacaagtcat ctaccaacat gcctatcatt
     1321 gcagagaatg tgaagcatta ggagaaacaa aaatcaaaaa agcgcctgtt cctaaaccgt
     1381 tgatcaataa tagtctaggt tcctcttcaa ttgtgacaca gagtatttgg gaaaaatttg
     1441 ttaaaaaggt gccggcatat cgacaagaaa aagaatggca tgcacttggt ttccctcttg
     1501 atcgtaaaaa aatcactaat tggcatatca aggtaagtga aatggtttta gagccaatct
     1561 acaattgcct tcatcaagaa ttaagcaatc aggaggtctt acacgcggat gagacaagct
     1621 atcgggtatt gaatactcat aaagaaaaga ccttttactg gttatatgct tcaggtaaaa
     1681 gcgaagaaaa acaagttgtg ctctacgagc acgcaaattc gcgttccgtt gatgagccaa
     1741 aaagtttttt agaatcattt agcggtttct tgcatactga tggttacgcg gcctatggaa
     1801 agctgccggt aaacaatgtt tattgttggg cgcatgttcg cagaaagttt tttgaagcat
     1861 tgggtaaaca accgtcccca aaaagtcatg ccaaaatagg attagacttc tgtgacaaga
     1921 tgtttcgaat cgaacggacc tttaataagc ttagcgcaga agaaaggttt gtagcgagga
     1981 aggaaacgtt gcagcctgtg atgactgaat tttttcattg gtgtgagagt tttgcttatt
     2041 tgccaaaatc gcagcttgga aaggcggttc aatacgcagt caatcataaa gacggacttc
     2101 agattgtgtt gcttgatgga cgcttggaac tttcaaataa ccgagcagag cgtgccatca
     2161 aagagttagt gatcggtcga aaaaactggc tattttctaa aagcttaaaa ggcgctagat
     2221 caaatggaat tatattgagt attatccaga cagctgttgc gaatgggtta aacatccgca
     2281 aatatttaaa tcatcttttt acagagattc ccaatctgtc ttcgatgaca cctgaagcgc
     2341 tacgcgctta tttgccatgg aatcagcaga tacaagaaat ctgtaaataa tataagccac
     2401 aaataacctg atttgagtgt atcaaatcgg gctatttgtg tttaggtacc attctatgac
     2461 acgcttacgc tcaaaactac tgaattatta agttatgtct aatcaaataa aaaaaccatt
     2521 ctcatatttg ctgaataatt agcatttaga gaatggtttt ttatctttaa aatctactac
     2581 tcaaaatctg catgtcgagc aaacatcgtt tctgagcttt cacctgtttt atccattaaa
     2641 gataacacta aactatcaaa ggtgagaaat gctaattgtt caaaacttga acccataggt
     2701 tgtgaaaaag tatcttcttc tcgttgattt tcatctttgg taatacctgg taatacaaga
     2761 acagaatctg ctaattgacc aatcgacgag tcttttttca tggtaactaa agcaatgctt
     2821 acaccacttt gatttgcttt ggccgctaaa ttttttaaac tacctgtttc acctgagcca
     2881 gaacagataa ttaataaatc tccaggttgc gaatgtggtg aactaatttc accgacaagg
     2941 ctgactgaaa aacctaaatg cattaagcga ttcgtaaatg cttgcatggc aattccagag
     3001 cgtcctgcac cagctagaaa gatgtgttta gcttgaacaa ttttctcact aaattgttct
     3061 aactctacag aattaatacg ttttgagtta ttttgtaatt ctaagataat cgcttctaaa
     3121 tagtttttat tttggtcttt attggtcatg atcttttact tcacttcctc taatgattga
     3181 tttagtttat caatttgttc ttgcggggta tttttcccat tgaagatgaa accgcctgat
     3241 acaaaaatat ccgcccctgc tttagaacaa actttaatcg tttgattatc aatcttgcca
     3301 tctacttcaa tatcatacgt ataatcattt tgtttgcgta ttacatctaa ctgttcgatt
     3361 ttaactactg tctcttgaat aaaatgctta ttctctgttc ctggattgat tgtcattact
     3421 aatacttgat cgactaaaga taatactggt tcaataaaac tgactggtgt tgcaggatta
     3481 aaagtaatcc ctacttttgc tcctgcttcc ttgactaact gaatgacctt cattgcgtgt
     3541 ttggtagctt caatgtggaa ggtaacaata tctgctcctg cttctacaaa tcttgtgaca
     3601 attttttctg gtgtatcaat catcaaatga acatcaaaca caacgtctgt tagtggacga
     3661 atatctttaa taacacttgg tccaaaagcc atatctggta caaaacagcc atccattaca
     3721 tcaatatgca aataatcaac attcttttct tttaagattt caaataactc tttaacttct
     3781 gcgataggtg catttaacaa tgatggagca attgtcattc tctattcccc cgtttattct
     3841 cccataatca tgagattgat tgttctagtt cgctctctta gttgatccca atcgacaaaa
     3901 tagacacggc ccatttctcc taataaaagt ttaccttctt gaacaattaa agtcattgag
     3961 ttggttccat aaatagatga acgtaaatgt gcatcggtat ttagcatgac ccatttttct
     4021 gcagggtaat tgggatcaga taatcttgtt ccaaaatcaa tgtgttttgg ccccggactg
     4081 ttgtattgat tttcactcgt catttttgga ataatcgttt ccattacatt gttgatgtct
     4141 acatgtaaat aatcatcacc aaaaaagttt gtgtcatgca tcgtttcttc aaaaaagagt
     4201 gagcaagttg tatgcgggct cgaaattact aacattccat tctcaatacc tgctgtatca
     4261 actgtatcta aaagttcttt tcgaatatca tgatacgaag gacgccctga aactgtttca
     4321 atagtcaaag tgtcatgatg aaaacgcata ttaaacacct aatctttctt ttacatctgc
     4381 ataaatggca tctgtaccaa tacctgttaa taagcccaag ccattcgtta ctttagcttt
     4441 aattacatca gggacaattg ttgtacttac tacagcatca tattcatcaa ttttatttag
     4501 ctcatcagca attttagatt gataaatttt cacttgatct gttaatcctt cttcttccaa
     4561 ccattttgat aattttgcta ctgcaattgt agaagtagca tgacctgctc cacaaataac
     4621 taataatttt ttcatattga tctcttcttt cttattttaa ttttatgatt aagctttctt
     4681 cactttgttc atgtagatag aagcacctac taaaacaact cctaatccac ctattccaat
     4741 tacaccaaag taagatgcta ataaaacaaa caaccctact ggccataatc cacataacaa
     4801 catagttact aatcctgctg tccccacatc gaaacttgct aatgtaaaca catcagtgat
     4861 aattggtgcc aaccatgttg ccatatataa tgttgtaaca acatacaacg aacatcctac
     4921 taatgatctc acaatatttc ctttaaaata aggaatcatt aaacagatgg cgaaagcaaa
     4981 gaatgctaaa tctccaaaag gtaatacttt atttcctgga agaattagtg ctagcaataa
     5041 tgagattggt acaagcaata gagaagttga cataatagtt ggatgaccca cagttaacgc
     5101 tgcatccatt ccaatgttta cttggcgacc atttaacttt ttattcgtaa attctttcgc
     5161 cgcttctgca attggcatta aaccttccat aaacattgct accattttag gcataatctt
     5221 catcactgcc gcagtcataa ttcctaagtt tccaattcct gctacgtcat agcctgccat
     5281 aatacccact acaataccga taattaggcc aataacaact gtgtcaccta aaacaccgaa
     5341 acgttttgtt aatgtttctg catctgcatc tattttatta aatccaggaa tacgatcaaa
     5401 aacccaattt actggtttgg caaatagtac tcctgataat cccatcatat gtgtgataga
     5461 gattcctgga tagccgaaaa attcgctaac agttttttgt gtacgatctg ctaagaacca
     5521 ttgtaaaatt actgcgacaa tcgcgccaat taagccaaac caaaagtttc ccgtaacaga
     5581 ttgagtaatc atacctagga aaattgcgaa ccaaaagttc caaatatcaa cattcaatgt
     5641 ttttgtccaa cccaaaacca caaatatgaa gttaagaatt aatgaaattg gaatcattaa
     5701 aattcccaat gatgtagagt aagccaaagg tccaccaaca cctacgccaa tatctaggat
     5761 attcaggcca ccaccgtatt gttcccccat ttgttgaatt gctgttccta aaccatctga
     5821 taataaactt aaaactaatc ctaaaccaat agaaccgata ccaacagtaa cgccggaaac
     5881 aaatgcttta ccaggtttta atccaaataa tatcccgatt acaaaaataa tcagtggtaa
     5941 aaagacgttt gaccctaatc caataaacca ttcaaaggca tttaatacaa tatccatctc
     6001 tctattcctc ctaataaact cctgcagaat gtactaactc aataaatgct tctttcgtgg
     6061 tacatgcaag taattcattt actttttctt cattttgaaa taggctaact aagttttgta
     6121 gtgtatcaat ttgttcatga ggttgtgaca ttgccaacat aaagactaaa cgtacaggta
     6181 tttcatgaga tttatctgtc atatctgaaa ataccagtgg ttcctttaat gaggcaaaac
     6241 agatttgaga gctatttaca tgtgcggaat ctgtatgtgg gatagcaaac ccaatttgat
     6301 ttacttctaa tccagttgga aattcttgtt cacggttggt gataccttct aagaaactat
     6361 ctttcacgca accagcgttt tttaattgtt cagacattac ttgaaacaat tctttttgat
     6421 ctttcacttc taattgaaat aaacataatt ctttatctag atacattttt attctccttt
     6481 ttgttgaaaa attcttagta atttttcgct atcatgaact tgtaacaagc gatcgaccat
     6541 ttctttttgc ccaataaatt ggtaaagttc ttcatatact cctctaatct cagatacatc
     6601 ttcttcagct aaactaacca taatgactaa tttgactttt cgatcgcccc aattaatcgc
     6661 attttttaat gctacaataa aaatgttgga ttcaatgacc attgatggat tcgcatgcgg
     6721 taaagcaaca ccactttcta aattagtatt ccccattttc tctctagaga agatagaagc
     6781 acgaaactct tcagtaacta atgaattggc ttctagttcg gaaatcatca aatccaaaca
     6841 ttcttctttg ctttccacct gtactcctgt ttttactaaa gatgattgaa caaatttaga
     6901 tatggtggga actttaaccg ctttaggaat cagttcattt tgaataaatc gcgaaggttc
     6961 ttctattaaa tatttagtat aagcttccat aatattcata taatctgttt tacttaccaa
     7021 tggagatacc cttactaagg gtataccacc tgcatcaaag gaaagagagg aaataatcaa
     7081 atcaacttcc ccaatttcag ctaaatgaaa cttatctaaa gttgatattt ctacattatc
     7141 ttttgctggt aaaaaccgtc gaactttatt gtaaattaat tgcgcagaag aaataccgta
     7201 ttggcaaata atgatgatat tatttgcttt ggactgatct tcaatagcaa tctgaaaatg
     7261 aatcaaaatt aaggaaactt catcgtccgt taaagtcaca tcaaattctt cttcaaggat
     7321 ggacatggcg taccatacta tgctaaagag ttctgaatat tgagatttga tattttctaa
     7381 taacggattt tgaattttaa tacctttttt taaacgaagg accattgctg gcacatgata
     7441 caataaggat tgatataatc gatcattttg agtcaaatca attttttcaa tctcactcat
     7501 tctttcaatc aatttatgaa cgctcatagc gtactcttca ttgctggttt ttaaattatt
     7561 tgtcacacga tgtgcaaata gttgacgaca tagatattct ttatctgcgt cataaaatgg
     7621 aagattcaat tttgccttca attcttcaat taaaccattt gccacaatgt acgtttccat
     7681 atagcgaatg ctattgaaga ggaattcttc ttcaacagtt aaatgatgac ctttcagtaa
     7741 acggttacat tggataacta aagtagaaat caatgaccga acatagtaat cagaaacctt
     7801 ttcagtcagt tcactgtaat cattgaatag caaatcatga atgacactaa taacttttga
     7861 atcaaacaac atatttaaaa cttgaaaaaa tgtttttgat tccatgtcat tcgcataata
     7921 gaatacaagt tgcttgattg ctcgttgaat tgcttgttca ttaccagtaa aactaattcc
     7981 ttctgcagat gactgcaact taacttctcc actgccaata attttattga taattctcag
     8041 gtcattgtat aacgatgttt tactaaccat aaaactttga gacaaatgag ttaaagaaac
     8101 aggcttatca ccaaacaaaa cttctttaat gatctcgaca cgtctttttt ctggagaata
     8161 aatatctttt tcaagataat tttctaccga tgaaatcaaa tgttcaacgt ttttcctatc
     8221 acctatgagt ttgataccca aagaaggtaa tcgctcaatt gttacttggt aattaactac
     8281 ataatcttcc aacaattcaa tatcttgata aatggtcttg gtcgacactg ccaatttatc
     8341 actaaaaaaa gaagcgggtt taaagtcttc ttgtcgactt aataatttta ataaatttac
     8401 atgtctttca atcaaacttg ttcacctctt aacgctagca tttgttacct cttttcatta
     8461 tttattctag cactggaacc cccttccctc aattacatct atgtcaataa tacttgtgga
     8521 agtttgttgt aaaggaaatc caaataatct taaaaaatga gaaacaaaaa aggagaaatc
     8581 cttaaaagag ctaagatatt ccaatagaaa atactcctta ttatggaagt aatatgaaat
     8641 attacaattg aaaaataaaa acaatataat ggcgtcacta aaattcgtat ttttttatta
     8701 taatataact taattacaaa tgctcatcta tgaacaattg agtccta
//
LOCUS       DACEOE010000101         8690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEOE010000101 DACEOE010000000
VERSION     DACEOE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.103"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            155..421
                     /locus_tag="IW774_001916"
     CDS             155..421
                     /locus_tag="IW774_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1154258.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            565..2556
                     /locus_tag="IW774_001917"
     CDS             565..2556
                     /locus_tag="IW774_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1154259.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            2786..4087
                     /locus_tag="IW774_001918"
     CDS             2786..4087
                     /locus_tag="IW774_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1154260.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(4217..5587)
                     /locus_tag="IW774_001919"
     CDS             complement(4217..5587)
                     /locus_tag="IW774_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1154261.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5769..6092)
                     /locus_tag="IW774_001920"
     CDS             complement(5769..6092)
                     /locus_tag="IW774_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1154262.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(6417..7022)
                     /locus_tag="IW774_001921"
     CDS             complement(6417..7022)
                     /locus_tag="IW774_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1154263.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            7352..8068
                     /locus_tag="IW774_001922"
     CDS             7352..8068
                     /locus_tag="IW774_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1154264.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            8081..>8690
                     /locus_tag="IW774_001923"
     CDS             8081..>8690
                     /locus_tag="IW774_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1154265.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNK"
BASE COUNT         2743 a         1585 c         1805 g         2557 t
ORIGIN      
        1 ttcacggtgt tccagaagca gcttctacgg aaataagctg aacacttttg ttacaacttc
       61 tttttttctg gagtttcttg gcgcatattt ttggagtcct tctgaataaa gcgtaaaata
      121 aaagtgagaa atatggataa aggagggaaa ggagatgctg aattttttat ggtcgttatt
      181 tatcggcggt atattaggtt tgatagctgg cgcaatttta ggaaaggaag tcccgggagg
      241 cgtatctgga aatatcatta tcggtttttt aggtagttgg ctcggcgaat ttttactagg
      301 accactggga ccggaactcg gcggttttta tttgatacca gcattattgg gtgcaatttt
      361 ttgccttgct ctttattcat ttgtggcaag acgtatcaga gaacgtccaa agaaagaata
      421 attttattca aataagttga cacttcagtg tgttacttgg ataattagaa gcaatttagg
      481 aaagcgggtg tcatggataa gaatatcatc tatcattcaa gcttacctaa aggatatcca
      541 tataacaaga aatgagggaa aaacgtggca acaacgcaca ataccaaaaa gatatcaatg
      601 gctggcctgc ttgtagcgat gggagtcgtc tacggagaca tcgggacaag tccgctttac
      661 gttatgaaat cgattgttga agataatggc ggattgtcag gaatcaatcc tgattttgtt
      721 atcggatcag tctcattgat tttttggaca ttgactctac tgacaacaat caaatatgtc
      781 gtgattgcat tgaatgcaga taaccatggt gaaggtggaa ttttctcact ctacacactt
      841 gtccgaaaag gtgggaaata tttgatcatt cctgcaatga tcggaggagc tgccttacta
      901 gcagatgggg tattgacccc tgctgtgacg gtaacgactg cgattgaagg cttgcgagga
      961 attccgcagt tttttgaccg gttcggtaat aaccagaacg tgatcgtcat tatcacttta
     1021 gttattattt ttgttttatt catggtccaa cgctttggta cagatattgt cggaaaagca
     1081 ttcggaccaa tcatgttcgc ttggtttact tttttaggcg taatgggatt aatgaacttc
     1141 agtcaagact ggacagttat ccgtgcactg aatccttatt atgctatcca gctgcttttt
     1201 agtgcagata acaaattagg cttgttcatt cttggaaata ttttcctggc tacgacaggt
     1261 gcagaggcgc tttattccga tcttggacat gtcggcaaac gaaatattcg tctgagctgg
     1321 ccttacgtca aaatttgttt ggttatcaat tattttgggc aggctgcgtg gttgttgaat
     1381 gtttatcaaa accctgaagc acagcaaatc aagaacctga atccattctt ccaaatgatg
     1441 cctcaaagtt ggatcgtgat cggcgttgct tttgcaacag tcgctgcagt cattgcttcg
     1501 caagcattga ttacaggttc gtttacgcta gtttcagaag caatcaagtt gaaactgctg
     1561 cctcggttaa agatcatgta tcctgggaac agtatcgggc aaatgtatat cccagcagtc
     1621 aatctgattt tatggatcgc ctgttctcta gtggttgtca cattccgaac gtctcatcat
     1681 atggaagctg cttacggtct atcgatcact gtgacgatgt tgatgacaac agcactgctt
     1741 tacttttatc ttatccaaaa tgggcattct cgttggcttg catatttagt tacctttttc
     1801 ttcggtgcga tagaagtcgt tttctttatc tcaagtgttg tcaaattctt ccgaggcggt
     1861 tttgtagccg ttctgattgg tttgatcatc ttagtcgtga tgtttatttg ggaacaaggg
     1921 aacattattc gcgaatcgtc aaccgaggat gtgtctttga gagattatat cccgcagctt
     1981 agagaattac gtgatgatac ttctttacca atctatcaga caaatgtggt cttcttggtt
     2041 cctgatatgg ctgaagataa agttggccga cagttcatct attcgatttt agataaacgg
     2101 cctaaaagag cacgagtcta ttggtttgtt catgtggaag tgacggatga gccatatacg
     2161 aaagaatatc aagtagatat gatggacact gattttattg tccaagtgaa tctgttctta
     2221 ggtttcaggg tccaacagga aatcaacgta tatgttcgcc aagtcatcca tgatttgatg
     2281 aaacaaggcc gtttgccaaa acagccacaa agatactctc tgacaccagg gcgagaagtt
     2341 ggcgatttcc aatttgtcct gattgatgaa gtagtttcca atgtgacaac tctaggaaaa
     2401 tggcaacgtc aaatcatgca ggcaaaactg gcaatcaaaa aaatcgctac ctcgccagaa
     2461 acatggtttg ggctagagta cagcgaagta aaacatgaaa ctgttccgct tatcattgga
     2521 aatactcgga aaacatggtt gaaagagcgg ttgtgaaagt agcgctttga cctgaactaa
     2581 tgtcgaatgt cagctacttg aacaccgttt atcagaggtc atgaaaaagc tgtttagcaa
     2641 caagtaacgc aatgattttg aagtgataag gagatgtggc tgaaaaatgc cgcatctcct
     2701 tttttattcc taaatatgaa tacgcattat tttcaatctt tttgtagaaa aagggtacaa
     2761 tagaaaaaga gaaaggcggg agaatatggt tatcaatttc attattttat ttttgatgat
     2821 tgcaattact tcattttttg tagcaagtga atttgcttta gtgaaaatca gacgatcaag
     2881 attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag ctagctcttc atgttacaca
     2941 ccatttagat aactatttat cagctagtca attaggaatt acgctaaccg gtttgatcat
     3001 cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa ccagtgatcg gtaaactacc
     3061 ttttagttcg gctattagca gtacaatctc tgtggcgctg ggatttattt tagttacgta
     3121 tatcgatgtg gttattggcg aactgcttcc taagagctat agtatcgtca atacggagaa
     3181 ggtcgtcttg ttcgtagtta aaccacttca ttatttttat aaagtaatgt ttccttttat
     3241 ttgggtattg aatcattcag ctgcagggct agggaagcta ttaggtgtgc gtctcgtatc
     3301 tgaaggagaa gagacattgt ctcaagaaga gctgacatta gtcgcattga attcttatga
     3361 aaaaggagag ttgaccaaag aagaatatca ttatttggaa aacgtgtttg aatttgatga
     3421 taccttggca aaagatatac aagtagaccg tacgtccatg caagtattcg aagcttcaga
     3481 aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga catactcgct atccggttat
     3541 cttagaatca aaagacaatg ttttaggtta tgtcaccttg ccagatttga tcaagcaatc
     3601 ttataaagat gaccagctga cagtagaaca gttgattgaa gagccgatcg taacaactga
     3661 aacgatccct ataaaaaaat tattgacgat catgcgaaaa aagggaaagc atattgctat
     3721 cttaaaagat gaatacggag ggacaagcgg cttagtgacg attgaagata ttttagaaga
     3781 aatcgtcggg gaaatccgag atgagacaga tttggacgaa gcattgatag cggaacagtc
     3841 agatggctct tatatcatct ccggcaaact aacattagat gatttccagc gttattttca
     3901 tgtagagatt ccagagtttg aagaaacgaa ttttactaca cttgcgggct ttgcttctag
     3961 ccgatataaa gaaataaaag caggaacaat cattgagatt gcatcattcc gtttcacggt
     4021 attagaatac cagcatgcac atatcgatta tttcaaagta gagtccacgg aaagaaaaat
     4081 agaataaaag aaaaaaggct gtgacatttt ttgtcacagc ctttttgtta tctaaaaaac
     4141 ctgttttcgg taaacgaaga agatattttc aataaaagtt ttcattttta gccgcaacga
     4201 tttcttttaa tcaaggctat cttgcttctg catgttctaa tgtgaaatgt tcctcttcac
     4261 ccatgtgttt caattgtttc atcgaacgca tataagagta aagcaacacg gcaacagaac
     4321 cgatccaagc taaaggagaa gctgctgctg ctcctgggta tccccacatt cctgtaagaa
     4381 cgatagctgc aaaagaacgc ataagcaatt ccatcatacc ggcgatcgta gggatcttgc
     4441 tttgtccaag cccttgaagt gtgtagcgta aaatgaacag gactgctaag atccagtaaa
     4501 gagacccatt gatattgaag taggtttgtg ctaattcaaa tacacgtgtt tctgatgggc
     4561 taacaaacaa ttgaacaaag aaatgaccga agaaaatgac gatagcacct gctaagagac
     4621 taaatccgac actcatcagt aaagtttgac gtaccccttg caagattcgg ccatattctt
     4681 tcgcaccata gttttgtgca gtgaaagttg ccattgcaat accaaatgac atcatcggct
     4741 gatttgcaaa ttggtcgata cgaccagcag cggcttgagc agctacgaca tctgtcccta
     4801 gactatttag tgctgcttgt aacacgatag caccaacagc gatgatcgaa gattgaaacg
     4861 ccattggcaa agcagcattt aggtgtacag caaactcttc tttttcaaat ttgaaatctt
     4921 ttttgcgtaa ctgtaacaaa ggcatttttt tcttgatata cactagacac agtacgctgg
     4981 aagaaacttg tgcgattact gtagcaatag ctgcaccttc cacacccatg ccaaaataga
     5041 taataaagat caaatcaagt acgacgttga tgatgaccgc aatgatcaga aagaatagtg
     5101 gtgtgcggct gtcaccaagt gcgcgaatga cattagaaag caaattgaaa ctgactgatg
     5161 caaagatccc taataaaata attgaaataa atgtttgtgc ttgatcaagt atttcaggag
     5221 gagtttgcat caattgaagc atcggacgaa taaacaggag acttaggaca gttaaaatca
     5281 cagtgacgac aagactaatg acaacactag cagcaaaact tttcttcaga ccccgataat
     5341 cttttgcacc gtaacgttgt gcggtaataa tggctaaacc agctgttaaa ccttgggcaa
     5401 agccaatgat caaaaatgtc aggcttccag ttgcaccaac cgctgccagc gcatttttac
     5461 ctagcgtctg acccacgatg atcatatcga tcatattgta gaactgctga aaaacatttc
     5521 cgatcagtaa aggaacagtg aacatcaaaa tcagtttagc ggggcttcct tttgttaaat
     5581 cgcgcatctt ttaaactcct tatatcttta aaatttagtt agtagtatat agcgtttatc
     5641 ctgagaatac caattagaaa tcaatgataa tttatgaaac tccataaaaa gaagccaaga
     5701 attgagtcaa agaatcaaca acttggcctg gtctgtaaaa taagggacat ccacgtttat
     5761 ctatcacctt aatattgtct aatctcactt ggtaaaagga tcgtttctct cgtaccgtca
     5821 tcataggcgt atacttgagc aggaaattgg ttcgaatagg caaacggcaa atcaatatcc
     5881 atttccacat tgcttcctgc ttgagaaaaa tgcatcgtca cttttccacg attttcgatc
     5941 aaatcaaagg ttaatatatt ttctaatgga atgactccct taagatctag gtcaataata
     6001 aaccatatac tgtcaatcaa ttcatctggc aaacttgata cgacaccaac tgaagcgtaa
     6061 cggttgcggc tgctatcaaa actttcaaac atctgtttaa cctccttcga atcaacgaat
     6121 gttttatttt tataattaat cctaggataa actagaaata cttacttcaa cgagggttca
     6181 ttctaataaa gaatcaggaa aaatgcaaca ataattgaat actttttttc aaagtataaa
     6241 tttttataaa ggtacgataa aagtatgcga ggaaaagctt ggtaaattag gatttttcgt
     6301 ttgtaaagcc atttacaaaa gtggaagccc catatgaaaa taaacgatta gaaataaaaa
     6361 aggcgtgtta aaagctgctg acccgccgct tctaacacca cctgattgtt tcagtattat
     6421 tcttctgttg aaccgatcac ttctggttcg ccaccttcac ctgtgctttc ttcttcactt
     6481 tgagcgacta cagcagcgat ttgctcttca ctgtcggtca cgaatttgta atcgtcgttt
     6541 tttggaagat cggctactgt aatagaatca cctaattcca agtttgtcac gtcaacttca
     6601 attcgttcag gcaatttgtc tggtgtagca gatacgacta ctgtatacaa gttttgagct
     6661 aacactccac cagatttagc acccactgat tcgccaacaa gaacgacttc tgtttcgact
     6721 tctgtttctt ccgacatgtt tacagataga aattctgcat gttcgaaatg attggtaaaa
     6781 gtgttcgttt ggatcttgtg aaccaatgta ttgacttttt gtccatcgat gtccatcgtg
     6841 atgactgtgt tcgctccatg ttcacgcaac aatttgctta gtgtcaaacc atcgaatgag
     6901 ataggggtac tttctacttt atacccataa acaacagctg gaactttccc ttcgtgacga
     6961 agtttgtttc ttaatgaacg gggacgtact tctcttttac ttacttctaa tgatactgac
     7021 ataaccaaaa ttcctcctta aaatgtgact gtctgctttg gtaacggaca gattttattt
     7081 aagtgaattc taaaggctaa tctcgtgcac atcatcgaag gcagggcgct taaaaaagcg
     7141 caaaaggaga gatgcgcgca tctcccctta gaaagtcagg attcaacttt tctccaccag
     7201 gtcattacgc tgtgtatgag aaatccaatt cactttacta tttaaccgta acgtgaactg
     7261 ttaattaagt caaggaataa ctatgatgat gtatttgcaa gcaagaatat tcaaggtata
     7321 attaattagt agtaataaga aaggaaacag catgcgttta gataaattaa tagaagaaaa
     7381 attagaaacg acacgcaagg aaatgaagcg gctgtttgct ttgaagcaag tgatgataga
     7441 tggcacagtc gaatttcgac aaaatcgaaa tgtagatagt ttactgcatc aaatcgaagt
     7501 agcaggtgaa cacttagaaa caaatgagtc ctattatctt ctcaataaac ctgaaaaagc
     7561 tgttacagct gtatcagatc cagaaaagac gacagtaatc gatctgattg caagtgccga
     7621 tcgtttccaa ccattatatc cagtgggaag actagataga gatacaacag gactattgct
     7681 gatcacttcg aatggacaat tgggatatga aatgctactt ccagaaaaaa aggtttcaaa
     7741 gacttatcaa gtagttgtga acgaaagggt gacaccggca gacgtaacag catttgaaaa
     7801 tggaatcgta ttcgctgatg ggtatcaatg taagccggct gatttaaaaa tactcaaagc
     7861 agacaagcac caaagtacgg ttttattgac gatccaagaa ggaaaattcc atcaagtcaa
     7921 aaagatgttc ttagcccaag gaaagaaagt gaccgcattg aaacggctaa gcatgggacc
     7981 attggaatta gatgaacagc taggagtagg cgagtatcga tcgttgacat tagcagagat
     8041 gaaaaagtta agtatttatt ttaaatagga ggtcaaaaag atgaaatata aaactttatt
     8101 gttcgatgta gatgatacat tgttagattt tcaattaact gaagccaatg ctttaagtgc
     8161 tttgtttgca gaacaaggaa tcacattgac accagaaatc gaaaccagct ataaggcttt
     8221 gaatcatcat ttatggcagg agtttgaaaa aggcagacta tcacgagaag aaataatagg
     8281 cagtcgtttt gggttacttt tccagcaatt tgggaaaaca gtagatagtg atcaaatgga
     8341 aaaacgctac cgccactact tgaaccaagg tcatgatctt gttccaggaa gtctagaaat
     8401 cctgaaaaag gtccatccgc atgctgatct ttatattgtg acgaacggtg tgtcgcacac
     8461 gcaacatcgg cgattaacag atgcaggaat gcttgattac ttcaaggata tctttgtctc
     8521 agaagctgtc ggagcgcaaa agccgatgaa agaatttttt gatcatgtct tttcccacat
     8581 tcctaatttt gataaaaagc aaactgtgat cattggtgat tctttgactt ctgatatcaa
     8641 aggaggaaat atggcaggga tcgatacgat ttggttcaat agaaataaat
//
LOCUS       DACEOE010000102         8637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEOE010000102 DACEOE010000000
VERSION     DACEOE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.104"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(74..1030)
                     /locus_tag="IW774_001924"
     CDS             complement(74..1030)
                     /locus_tag="IW774_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1154266.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1134..1736
                     /locus_tag="IW774_001925"
     CDS             1134..1736
                     /locus_tag="IW774_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1154267.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1824..3323)
                     /locus_tag="IW774_001926"
     CDS             complement(1824..3323)
                     /locus_tag="IW774_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1154268.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3339..3863)
                     /locus_tag="IW774_001927"
     CDS             complement(3339..3863)
                     /locus_tag="IW774_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154269.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4161..4910
                     /locus_tag="IW774_001928"
     CDS             4161..4910
                     /locus_tag="IW774_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1154270.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4901..5686
                     /locus_tag="IW774_001929"
     CDS             4901..5686
                     /locus_tag="IW774_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1154271.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5655..6371
                     /locus_tag="IW774_001930"
     CDS             5655..6371
                     /locus_tag="IW774_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1154272.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6473..7492)
                     /locus_tag="IW774_001931"
     CDS             complement(6473..7492)
                     /locus_tag="IW774_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1154273.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7511..8569)
                     /locus_tag="IW774_001932"
     CDS             complement(7511..8569)
                     /locus_tag="IW774_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1154274.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         2593 a         1756 c         1544 g         2744 t
ORIGIN      
        1 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
       61 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
      121 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
      181 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
      241 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
      301 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
      361 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
      421 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
      481 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
      541 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
      601 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
      661 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
      721 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
      781 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
      841 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
      901 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
      961 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
     1021 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
     1081 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
     1141 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
     1201 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
     1261 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
     1321 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
     1381 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
     1441 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
     1501 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
     1561 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
     1621 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
     1681 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
     1741 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
     1801 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
     1861 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
     1921 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
     1981 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
     2041 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
     2101 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
     2161 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
     2221 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
     2281 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
     2341 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
     2401 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
     2461 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
     2521 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
     2581 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
     2641 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
     2701 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
     2761 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
     2821 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
     2881 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
     2941 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
     3001 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
     3061 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
     3121 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
     3181 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
     3241 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
     3301 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
     3361 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
     3421 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
     3481 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
     3541 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
     3601 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
     3661 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
     3721 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
     3781 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
     3841 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
     3901 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
     3961 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
     4021 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
     4081 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
     4141 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
     4201 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
     4261 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
     4321 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
     4381 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
     4441 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
     4501 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
     4561 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
     4621 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
     4681 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
     4741 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
     4801 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
     4861 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
     4921 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
     4981 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
     5041 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
     5101 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
     5161 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
     5221 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
     5281 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
     5341 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
     5401 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
     5461 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
     5521 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
     5581 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
     5641 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
     5701 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
     5761 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
     5821 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
     5881 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
     5941 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
     6001 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
     6061 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
     6121 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
     6181 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
     6241 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
     6301 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
     6361 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
     6421 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
     6481 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
     6541 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
     6601 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
     6661 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
     6721 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
     6781 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
     6841 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
     6901 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
     6961 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
     7021 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
     7081 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
     7141 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
     7201 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
     7261 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
     7321 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
     7381 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
     7441 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
     7501 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
     7561 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
     7621 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
     7681 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
     7741 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
     7801 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
     7861 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
     7921 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
     7981 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
     8041 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
     8101 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
     8161 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
     8221 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
     8281 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
     8341 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
     8401 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
     8461 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
     8521 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
     8581 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaa
//
LOCUS       DACEOE010000103         8405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEOE010000103 DACEOE010000000
VERSION     DACEOE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.105"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            259..1011
                     /locus_tag="IW774_001933"
     CDS             259..1011
                     /locus_tag="IW774_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1154275.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1033..2187)
                     /locus_tag="IW774_001934"
     CDS             complement(1033..2187)
                     /locus_tag="IW774_001934"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1154276.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            2372..4813
                     /locus_tag="IW774_001935"
     CDS             2372..4813
                     /locus_tag="IW774_001935"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1154277.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(5143..5847)
                     /locus_tag="IW774_001936"
     CDS             complement(5143..5847)
                     /locus_tag="IW774_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154278.1"
                     /translation="MKKKQKQELLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(6034..6279)
                     /locus_tag="IW774_001937"
     CDS             complement(6034..6279)
                     /locus_tag="IW774_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154279.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(6296..7147)
                     /locus_tag="IW774_001938"
     CDS             complement(6296..7147)
                     /locus_tag="IW774_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1154280.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            7313..8317
                     /locus_tag="IW774_001939"
     CDS             7313..8317
                     /locus_tag="IW774_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1154281.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         2713 a         1475 c         1729 g         2488 t
ORIGIN      
        1 gagttggcag gagaacaccg tttaaggaag aagttgtaaa atcagcattt tgagctaata
       61 ctgagggtca tctgattgga cactgtttat tcggaaagag ggactttgac acgactttgg
      121 tcacagtccc tttttttatt tatgatagac ggaaagccaa tcattttatt attcatcaaa
      181 agagatagaa tagataaaag aggagaatga tttattccga aaagtgaaaa catataacaa
      241 aaaattagag gaaatcaaat gaatgaaaaa gaatgggatg aatttgctga agaatattat
      301 gaaatccagc aagaatcgca agtaactatt gctgaagatg taaaaaatta tctgaaaacg
      361 gagaaagtcc tgcctgctga aacagcagca gacgttgctg gaggatctgg cagatttctt
      421 tccttagcaa aagaaattaa tcgctatgat ttgatcgatt tttcttcagg aatgctagaa
      481 aatgcaaaaa aagaagcaga aaaaaatgag ttgccaaatg tttcctttat caaacagtcg
      541 ttagaagaat ttatccaatc aaatgagaca tatgaaatgg tttttacagc agcaaatcca
      601 gcattggtac atcttattca attagaaaaa ttgatgaaaa agtcaagcaa atggtgcgtg
      661 ataatcagag tagtggaatc aatcgatgat gttttttatc ctatcgaaaa gcaactagga
      721 atgatagaag aagatcctaa tacatcgcca gttgtaatgg atcaatttga aacatactta
      781 attgatgaag gattcaaagt gaagaaaaaa gattttactt attttacaca ggaatcaatt
      841 actgaagaat tgatgaagct atactatgac gcgtacgcaa aagatcctgt tgtgaccagg
      901 tacattcgca aactattcag tgaaaaacga gaaatcctta gtacgactaa gctgacctat
      961 agactgctcc tttggtccgc agataaaaaa agaacctttc acgactcgtg aagggttctt
     1021 tttatgattt tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa
     1081 aggtacatct gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat
     1141 cgtttcatat tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg
     1201 tgcgaagaga tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc
     1261 accagaaccg tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc
     1321 cagtagagaa gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct
     1381 gtaacggatg ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg
     1441 gtctaatacg ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat
     1501 cgcctgataa tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg
     1561 gaatgattcg atatacgtag aattagataa aggaccatca acaacaggaa attcgctata
     1621 atctggacgc cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga
     1681 taaaatacgc gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc
     1741 accaccgctt gccaagccgt aacgagcaat atcacttgct atgactagta cttttcgttc
     1801 tggatgattt ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc
     1861 ctcttttact tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac
     1921 cgcactcgct tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag
     1981 ctcacggtct ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc
     2041 ttcatttgca cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg
     2101 atcatcccca cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa
     2161 ggaaagacga tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta
     2221 ctttactaag gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt
     2281 gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa
     2341 gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc
     2401 aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg
     2461 cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca
     2521 agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc
     2581 tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc
     2641 tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt
     2701 gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga
     2761 aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt
     2821 ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag
     2881 agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc
     2941 cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga
     3001 ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt
     3061 ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt
     3121 ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat
     3181 caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc
     3241 cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat
     3301 caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga
     3361 aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc
     3421 acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc
     3481 ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca
     3541 aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag
     3601 tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac
     3661 actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc
     3721 aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac
     3781 agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg
     3841 cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta
     3901 tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga
     3961 aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt
     4021 catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat
     4081 gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt
     4141 ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc
     4201 tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat
     4261 tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac
     4321 gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac
     4381 acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt
     4441 aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt
     4501 tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt
     4561 agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa
     4621 cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca
     4681 agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa
     4741 aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat
     4801 ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac agttatttta
     4861 tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta gaatttttta
     4921 tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca ggtagaagag
     4981 aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc tgatatttga
     5041 gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat gactaaaaag
     5101 gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat cagtaatcgc
     5161 tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt atttccgttc
     5221 gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg ctaatttacg
     5281 aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt ctgtgtaaaa
     5341 tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg cattcgtatc
     5401 agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt cttctggcac
     5461 actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt catcgttttt
     5521 caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac ggaatcgttc
     5581 gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt tgtcatcgat
     5641 tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg ttaagatttg
     5701 acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca agctctcttc
     5761 ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga attgatttaa
     5821 taattcttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc ctttcgatta
     5881 taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta tacgcaaatt
     5941 acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa atggaacgga
     6001 ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca aggcaaaaac
     6061 aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca ttagcgaatg
     6121 atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc ttgcaggaaa
     6181 atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag tctgggtaac
     6241 ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt tcgacttaaa
     6301 attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga tcgatttgtt
     6361 tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg attctgcttg
     6421 gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc aaagcaactt
     6481 gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca tcgaagattt
     6541 taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt gcatactcaa
     6601 ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc ggcataattt
     6661 ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca ccgattgctt
     6721 tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca tttgcttgtt
     6781 cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga tctaaataat
     6841 ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca tagctgtggt
     6901 ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca ctttccttaa
     6961 tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata tggcgataac
     7021 ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt gtttgccatg
     7081 tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg cttgttaatg
     7141 aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc atttttcgaa
     7201 aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa tgattttgat
     7261 tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga aaatggatat
     7321 ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc cagtcttttc
     7381 acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa agaagatcca
     7441 aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca tgatcgttga
     7501 atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg ttcagcggtg
     7561 gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg ttgtcttgta
     7621 tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg aggcttacac
     7681 tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga atcgagcaat
     7741 tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga ctgattggac
     7801 ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt atttttctgg
     7861 aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag tgatccaaaa
     7921 agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca tggctgattt
     7981 gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg agatgattgc
     8041 ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag aagtacaaga
     8101 gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt ctgagaaaaa
     8161 atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac cagtagatgt
     8221 atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga tatccgtgat
     8281 gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat attaggtgtt
     8341 caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa agacgaatat
     8401 aacaa
//
LOCUS       DACEOE010000104         8342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEOE010000104 DACEOE010000000
VERSION     DACEOE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.106"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            156..2627
                     /locus_tag="IW774_001940"
     CDS             156..2627
                     /locus_tag="IW774_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1154282.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2730..4466)
                     /locus_tag="IW774_001941"
     CDS             complement(2730..4466)
                     /locus_tag="IW774_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1154283.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4463..5167)
                     /locus_tag="IW774_001942"
     CDS             complement(4463..5167)
                     /locus_tag="IW774_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1154284.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5298..5801
                     /locus_tag="IW774_001943"
     CDS             5298..5801
                     /locus_tag="IW774_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1154285.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5761..6099
                     /locus_tag="IW774_001944"
     CDS             5761..6099
                     /locus_tag="IW774_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1154286.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6120..7538
                     /gene="ffh"
                     /locus_tag="IW774_001945"
     CDS             6120..7538
                     /gene="ffh"
                     /locus_tag="IW774_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1154287.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7650..8228
                     /locus_tag="IW774_001946"
     CDS             7650..8228
                     /locus_tag="IW774_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1154288.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8232..>8342
                     /locus_tag="IW774_001947"
     CDS             8232..>8342
                     /locus_tag="IW774_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HAQ1154289.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTY"
BASE COUNT         2707 a         1530 c         1760 g         2345 t
ORIGIN      
        1 caacacaaaa tattatagaa cccttttttt ttgctatatg acaggaatga aaggtgtttg
       61 caagcttaat gtgaggaaca ctttccatat ctgttctata ctggatagga gaagtttgga
      121 aatatccaga aaacgaaatc aatcgaggta gccacatgaa gaataaatgg ataaatcgaa
      181 gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt catccgtctt tttccatcgt
      241 ataaagaagg attaacggca gatcaagtca aggaacgaat agaaaaaggt gcagcaaata
      301 attcagtcga tcccacattc aaaacaaatc agcaaatcgt attggaaaac atatttacgt
      361 atttcaattt gatctttctt atcttagcca ttttgctttg tttagtagaa tcctataaga
      421 acttgacttt tttaccagtt atcatagcca atacaggaat cgccattttt caggaaattc
      481 gatccaaaaa gatcttggac gaactaaatg ttttaaatac agcaaaagtc aatgtcatac
      541 gagacgggat agaacaaaca atagatatag aagaccttgt aatagatgac attgttattt
      601 tagagactgg atatcaaatt ccagcagatg cagaggtcgt ggaagggaaa ctgcaagtta
      661 atgaggcttt attgacaggc gaagcagatg aaataaccaa agaaataggt gatccattaa
      721 tgtccggcag tttcattgtt tccggaaaag cttacgcccg attagacaaa gttggagcag
      781 attcgtatat ttcccagctg acagtcaaag ccaagacaat gggagaagga gaacagtcgg
      841 agatgattgc ttctcttaat caattgatca aatggattgg gatcatcatt attcctatcg
      901 gtatcctgct tttttctcaa agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg
      961 ttgcaatgga agcagcattg atcggtatga tacctgaagg actgtacttg ctgacgacga
     1021 ttgcattagc attaagtgcg actaaactag caaagcaaag agttctttta cacaatatga
     1081 aaagcattga gacattggca agggtaaatg tcctttgtgt agataaaaca ggaacgatca
     1141 cagaaaataa aatgtctgtt caaaaaatga tcgtctctaa aaaacaagaa ataatcagtc
     1201 agtcagatca attggaagca tgcattgctg actatgcaaa agcgatggca ggagacaatg
     1261 caacaatgga agctgttaaa gcatatttta caaaaaccac tgatcaatcc tatcaccatg
     1321 ttattccttt ttcttctgtc caaaaattca gcagtgtagc gttggcagac aaggtgtatg
     1381 ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg
     1441 tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg
     1501 aagaaccagt attgacagaa gcagtcacac cattaggcta tattctgatt gctaatccta
     1561 tcagaaaaga agcaagagcc acatttgatt acttcaaaaa acagcaagta gccatcaaag
     1621 tgatttctgg agataatccg ctcaccgtat ccaatgtagc tgtccaagca gggatttcac
     1681 atgctgatca gtacgttgat gcatctaaat tatcagaaga agaatatgaa gcagctgtcg
     1741 aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga
     1801 taaagcgtaa caatacagta gctatgacag gtgatggcgt gaatgatatt ctagccatga
     1861 aagaagcaga ctgcagtata gcgatggctt ccggaaatga agccgccatg caggcctcac
     1921 aagtggtctt acttgattct gacttttcac gcatgccaga agttgttgca gaaggacggc
     1981 gcgttgtgaa caatattgag cgatcagcca gcttgttttt agtgaagaat attttctcct
     2041 ttttgctctc actgttttca gtgatctttg ctctgaccta tcctttagaa ccatcacaaa
     2101 tcacattgat tagtttgttt actatcggac ttccatcttt cttgcttgct ctagaagaaa
     2161 acaaaaagcg aatcagaggg aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg
     2221 gactaacaga tatgatcgta gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa
     2281 ataaaacaga tgtttctaca gcatctacca tgcttttgat tgcagtaggg ttcctcgttc
     2341 tctataaaat ctgttcaccg ctgaataaat tcagaagtca gatcattctt ttctgtgcta
     2401 gcggtaactt tttttcggtc atctttttgc ataagctgtt ttcaatcacc ggtatctcag
     2461 ctgtatcact tttgctgttg agcattctat ttttctcggc agattcgatt tttaggcatt
     2521 tgaccacatt ggtagaatat ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta
     2581 gtcgcgtagc taaaaaattt ctatcgatat ttaaacgaga aaattgacga aaagtatctc
     2641 aaaaaagagt aaaaaagaac aaccactcgt gtatctttcg aagcaggatg agtcatccaa
     2701 ctttaaaacg cacgagcagt tgctcttttt tacattttcg gaatggtaac ggtgaaagtc
     2761 gaacctgttc caagatgact gtctacagta atcgtaccat taaggagttg cacgtagtct
     2821 tttacaatcg ctaaccctaa tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg
     2881 cggtaaaaac gctcaaagat ccgttcctga tcctcaatgt cgatacctat accaaagtct
     2941 tggacagaaa acgatagatc atctgttgcc tgatagcgga taataatctc gctgtcgctt
     3001 tttgaatatt ggacagcatt ttcaatcaga tttttgataa ttggataaag gatatctgtt
     3061 ttagtagtaa atggttcgtt ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg
     3121 attggctgat aattttgaac gatttgctgg aaataaggtt ctgtataaat tgtctggatc
     3181 tcgtaagaat tctcgccatc ttttgacagc tggatgattt cccgaatcag tttatctaac
     3241 cggatcgcat ctttttgcat gatctcaaga aaagaagtca gagtttgcgg gtcatctttt
     3301 gcaccatcca gtaaagtctc ggtaaaacca atcagtgaag tcacaggagt ttttaattcg
     3361 tgagagacgt tgccaacgaa atccttttgt agcttttcta aacgtcgcac tttcgttaag
     3421 tcatacgcca cccctagaat ctgtccggta ccttctgaat tagtaaagta gcgcaaactc
     3481 aagtctaatg tctgccgacc ttctgtaagg gtgatctcct gatggatcag cggatgctca
     3541 ggagtcactt gatgaatcag ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc
     3601 tgttcaggtt gatagcttac cacgtttaaa tgtagctgca tctttggatt aagcagtagt
     3661 aaacgagaat cagcatcatc aatcaggaat acgccaatca tcagttcgtt cagtaacgta
     3721 tataattgct cttctgtggt agtacacgcc cgatacatct tactcatttg ttcgctcaac
     3781 gcattaatcg tctgatacaa ttcttcccat tgatcgggtg tttgcatgat tacttccgcc
     3841 tgattaggat tttgcaccat tttttttagt acgggcagca cggttttcaa tggctggttt
     3901 ttttgataga tcagataata gatcattcct gcaagaacaa ggaagaaaga cagaaagaag
     3961 aaaaagaccc aacggcgaaa actctcagtt ctcggtaaaa aaacgcttgt cggttctgcg
     4021 atacgtaaaa tggcttctaa ctggccactt tttttgactg gtaatgcgac atataaaaga
     4081 tcctcattca aagtagtact tttgcgcaaa gcgctaccta ggttcccacc attcaatacg
     4141 gctttgattt cgggacggga gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat
     4201 aagatctttc cagtagcatc cattaaagta atccgttcgt tcgactgatg gacgaattct
     4261 tccaaagcag ttttgttttg cgctgcttgg aggttttcga catctaattg ccggatcagc
     4321 agttgtgctt tttttgtcag gtagctttct tgctgttcca cgatctgctg ttgaaaataa
     4381 tgggaaatca attgccagcc accaaaaaac aaactaacta aaagaataag aaagaagaga
     4441 cgttcataaa tttttttcca tttcatcgtt tgggttcctg aaacttatag ccaaatcctc
     4501 taatagtcac caagtacttt gggtgttttg gatctcgctc gattttttct cgcaaatgac
     4561 taacgtgtac atctacgatt cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta
     4621 gcaatgtgtc tcggtcgatc acacggtctt ttcgtttcat gaaataaaca agtaattcaa
     4681 attctttcgg tgttagttca atcagatggt cgtctacggt cacttgataa ttggtcaaat
     4741 cagcagtgat ttgtccaatc gatagatatt caggctcagc ttcatctttt gattgcttac
     4801 gaggttcaat tcgtctgaat attgctttca tacgggctaa tacttcgcga ggactaaatg
     4861 gtttggtcaa atagtcgtca gcacctattt ctaggccaat gatccgatcc acttggtcat
     4921 ctttagccgt cagcatcaaa atcggtgttt catttttttc ttgacgcagc cgctgagtga
     4981 tctccatgcc atccatcgtt ggaagcatca catctaaaat gatgaaatca aatgattgtt
     5041 ctaatgctaa ttctaatcct tcgcctccat ctgtggcact ggttaccttg tagccttctt
     5101 tttccaaatt gaacgtcaag agcgttacaa tagaaggttc atcatcaaca actagtattt
     5161 ttttcattca aatctccttt aaataggttt cattctgttt atattttagc atagaatcag
     5221 tacaaagggc tattttacaa aagaaataaa tggtataata ggaaggcact ttgctgaata
     5281 gaaaggaaga gcagtcaatg attggaatca gtgcatgttt aggcggtctg gcctgtcgct
     5341 atgatggaca gaaaaaagaa gtaccagaat tgatggaact agttgaaact ggagaagctg
     5401 tcatggtctg tccagaagtg atcggcggat taccaattcc tagagatccc gctgaaatca
     5461 ttggtggaaa tggattcgat gtgtggaaag atcaagcaaa agtatggacg acaagcggcg
     5521 aagatgtaac ggaagcgtac aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa
     5581 aacacattga tctagtcatc atgaaggaaa aaagcccttc atgcggaacc cattcgattt
     5641 atgacggaag tttttccgga ataaaaaaaa atggtgccgg agtggctact gcttatttta
     5701 ttcagcaaaa aatgactgtg ctctctgatg aagaatggct gaaaactaga ggtgagcaga
     5761 atggaaatcg aaaagaccaa tcgaatgaac gcgctctttg aattttattc gaccttattg
     5821 acagaaaaac aaatgaacta catggaatta tattatgcag atgatttttc attaggtgag
     5881 atcgcggaag aatacgaagt aagccgacaa gcagtatatg acaatatcaa gcggacaagc
     5941 aagatcttgg aggattatga aaaaaaactc catctttttt ctaattatat tgttcgagaa
     6001 caggtattgg aaaatatgac tgcatatatt acagataagt acccggaaga taaaaaacta
     6061 cttgaatatg tacagcaaat tcaagcaatc gaagaataaa taaaaggaat gaagaaaata
     6121 tggcatttga aagcttaact gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag
     6181 gaaaagtatc agaagctgac gtaaaagaaa tgatgcggga aatccgcctt gccttactag
     6241 aagccgatgt taacttgcaa gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg
     6301 taggggcaga agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg
     6361 aagaattgac gatcacactt ggttctgaaa cagcagagtt aaataaatca ccaaaaatac
     6421 caactgttat catgatggct ggtcttcaag gggcaggtaa aacgaccttt actggtaaat
     6481 tagccaatta tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat
     6541 atcgaccagc tgcgatcgac caattgaaag tattaggtca acagctagac gttcctgtat
     6601 ttgatatggg aactgaagtc agcccggtag agatcgtccg tcaagggatg gagttagcaa
     6661 aagaaaagaa aaacgactat gtattgatcg atacggcagg acgtctgcat atcgatgaaa
     6721 cattgatgga tgagttgaaa caaatcaaag aattgacgca accaaatgag attttgcttg
     6781 ttgtcgatgc catgactggt caagatgccg tcaacgtagc tgatagcttc aaccagcaat
     6841 taggtattac aggggtcgtc atcactaaac ttgatgggga tactcgaggc ggggcagcac
     6901 tatcgatccg ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt gaaaaattga
     6961 ctgacttaga agtattccac ccagatcgta tggctagccg tatccttggt atgggggata
     7021 tgctgacatt gattgaaaaa gcgcagcaag actacgacga gaaaaaagcg gaagaacttg
     7081 cacagaaaat gcgggaaaat tcattcgatt tcaatgactt tattgaacag cttgatcaag
     7141 tgatgggtat gggcccactg gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc
     7201 ctggaatcga aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa gcgatggttt
     7261 attctatgac gccagcagaa agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc
     7321 gaattgctgc cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca
     7381 aggaatcaag aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg
     7441 accaaatgtt tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga
     7501 tcaagaaaaa taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta
     7561 cagactgaaa atgtctactg ccggagcttt tctatcagca atttttattc tttactaata
     7621 caattactct aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa
     7681 gtatgcggca atatcatgat actgtctggg gtgttcctga atatgatgac caaaagctgt
     7741 tccgaaaatt gatgcttgat attaatcaag ccggattgag ctggcagacg attttaaata
     7801 aaagtgaagc attcgatgaa gcatatgatc gattcgatat caaaaaagtc gcaaattatg
     7861 gagaaacaaa aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa
     7921 tcgaagcagc cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt
     7981 tttcagagta tctttggtcg ttttctaatg gaaaaatcgt tgattcttct tatcattcac
     8041 aagaagaagt cccaacaaca agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa
     8101 gaggatttaa atttatcgga agcacaacaa tctatgcttt tttagaagct gtcgggatca
     8161 tcaatgatca ccttgacagt tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg
     8221 gcgaataaag catgcgaaaa atcatatttt atggtgctat aagtttagat ggctatttgg
     8281 caacgaaaac ggatgatctt caatggcttt ttgacactcc aacaggtaag caaactactt
     8341 at
//
LOCUS       DACEOE010000105         8173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEOE010000105 DACEOE010000000
VERSION     DACEOE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.107"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..283
                     /locus_tag="IW774_001948"
     CDS             <1..283
                     /locus_tag="IW774_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1154290.1"
                     /translation="KLMSPLQHIIEDDEGLYGIDEILALSIVNVYGTIGFTNYGYIDK
                     VKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKEPEKRSRLTQ"
     gene            complement(313..972)
                     /locus_tag="IW774_001949"
     CDS             complement(313..972)
                     /locus_tag="IW774_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1154291.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(974..1738)
                     /locus_tag="IW774_001950"
     CDS             complement(974..1738)
                     /locus_tag="IW774_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1154292.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1746..2423)
                     /locus_tag="IW774_001951"
     CDS             complement(1746..2423)
                     /locus_tag="IW774_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1154293.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2460..3845)
                     /locus_tag="IW774_001952"
     CDS             complement(2460..3845)
                     /locus_tag="IW774_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1154294.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3814..4068)
                     /locus_tag="IW774_001953"
     CDS             complement(3814..4068)
                     /locus_tag="IW774_001953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154295.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(4055..4139)
                     /gene="ffs"
                     /locus_tag="IW774_001954"
     ncRNA           complement(4055..4139)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW774_001954"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(4162..4677)
                     /gene="tadA"
                     /locus_tag="IW774_001955"
     CDS             complement(4162..4677)
                     /gene="tadA"
                     /locus_tag="IW774_001955"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1154296.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4784..5641
                     /locus_tag="IW774_001956"
     CDS             4784..5641
                     /locus_tag="IW774_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1154297.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5722..6057)
                     /locus_tag="IW774_001957"
     CDS             complement(5722..6057)
                     /locus_tag="IW774_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154298.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(6124..7494)
                     /locus_tag="IW774_001958"
     CDS             complement(6124..7494)
                     /locus_tag="IW774_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1154299.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7572..8108)
                     /locus_tag="IW774_001959"
     CDS             complement(7572..8108)
                     /locus_tag="IW774_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1154300.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2346 a         1733 c         1335 g         2759 t
ORIGIN      
        1 aaaattgatg agtccgttgc aacatattat cgaagatgac gaaggccttt acggtatcga
       61 tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg atcggcttta cgaattatgg
      121 ttatatcgac aaggtgaaac ctggtatttt aaaagaacta aacagccacg atggcgttca
      181 tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc gctgctgctg ccagtcgatt
      241 agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa taattcaaca aaaaaagaaa
      301 gaaaacgtct atttagacga gttctttctt ttttgtttgt ttcaagctat tttttcctaa
      361 gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa aataagataa agctatgcat
      421 gaaaaattct tctggaagta cattgatgat tggatcagta acgggatcga ataaccagtc
      481 atcgttgttg aaaaacgttt catgaaaaag gataaaaaag cgatcaaatc ctatcatcat
      541 gaaaaaaccg aaaacaactg gcagtaacat gccgatttga aacggacgaa tcaaccgcca
      601 aagccgatca ttcttttgca aatatttgat aaacaaaata ctcgggatca aagtgatgaa
      661 aaacacagca taatccacta aaaacaaaat tttcacttca taaaagtgat gggctccgct
      721 agcagaaact gggaaatctg gcaagctgag gatggttttg aagggattat tgagataatt
      781 cattaggtga tcaaaatttt tcagtaacgt ctcttgatct aaagaagtat aatccaaaat
      841 ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag ttgatcgtca caccaactgc
      901 cagactgatc agtgtcagaa ataggctgaa caatccagca tattccatcc actgccatct
      961 cgaatttttc atattagaag ctccattcat ccagtgattg tttcacatag gtagctggga
     1021 taggcaaccc aggtacatct tcttcttttg taaaacctga taaaacgagc aaagtatcta
     1081 tattattgcg tatcccagct tggatatctg tttcatagtt atctccaacc atgattactt
     1141 cttctttttg cagccctaat acttcaacgg ctttatccat aatgatggct tctggttttc
     1201 cgatgtagat cggcggcgtt tgggttgctg tctctacaaa agagatgact gaccctgctc
     1261 caggaagaag gccgcgttca gtaggtatat tcttatctgg gttcgttcca atgaaagtag
     1321 cacctttttg aatagcaagt gttgctttaa ctactttttc ataggtcaga taattatcta
     1381 agcctaccac tacatagtca ggagcctctt cttcccatgt aaatccggca gctaaaatca
     1441 agtcaatcaa tcctgcttct ccgataacat agactttttt cccttttcca tctgctttca
     1501 taaaatcgat tgttgccaaa gtagctgtgt agactgtctc tggagctaca tgaatgtcaa
     1561 actcatctgc tagacgatta gctactgttt ctggtgactt agtcgtgtta ttggtaacaa
     1621 acaagaaagg taattttctt ttttgcaatt cttccacaaa tcgtttaccg gccggtatgg
     1681 gttgtgtccc acgataaatc gttccgtcta aatcaattaa atatccttta tagtccattc
     1741 atccactact cttccctttt tctgatcgta aatgctgttt ttgttttttc tacttgttta
     1801 ggttgttctt gtttttttac tttttcagag gtttttgctg actctgtttt tcgattttta
     1861 ataaccggtt ttttgcgatt agatgcgact ggtccttttt tctcatcgat atacgcacga
     1921 ttatggtttt ttttcttttt tcggcgctga gttgtctttt cttttttacc gccgattctc
     1981 tcgatgacaa aatacgcaca accaaaattg caatattcgt aaagatagtc ttccaaggaa
     2041 tcgattcgct gatctggatg cgcccgtcga ttatccgctt ggaaaaatcc ttttaggcgt
     2101 agttgttcat aaccccaatc tcctacgata taatcatatc gggctagtac atcactgtaa
     2161 cgttctccaa gcttttctgc attaaatcct tcacgataat cggacacaag ccgataatgt
     2221 ctttcaccta tcaaaaaatt actgtcatct aacagtgtca ctagctcgcc tttgactttt
     2281 tctgctttag gctcctcaac gatttcttcc aaagcagctg tcagttcttc ggtgactgtt
     2341 gtttcctgtt cagctgatga ttttttctta gcaatttctg ttttatgctc aacgttctgc
     2401 tgttcatttt gttttgcaga catgcgttca ccacctatct tttttattat accttgtttt
     2461 tatttgattg ggtagtgagc tttcaagtaa tctcctacca cacttcggat aaattcaggg
     2521 aacaaaaatt cattttttcc agcaatctca atcgttggaa aaaatggaac aaacatcaaa
     2581 tgatctacgg tagtaaaagt ataattttta ttcagatcga ttggcttgtt cttccaaaag
     2641 acttgatggt taatagaatc atatgtgatt ccattataga ccatctgccc aaaaatctta
     2701 ccgcgaaatc ccattccctt cataggatag ttacgtaaaa agttacgatt tttttctatt
     2761 tctaaaacta agcggatcag atcatttcca cccaatgtga catttaacaa gtgcatcgga
     2821 tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc cttttggaat ttcagttaaa
     2881 aacagaccac tgtttaatat tgctgcttcc gtctttcctc gttctttcac cgcctcaaag
     2941 gctacttgga taaacgaatg ctcattgaag atatctagac tcagagaaaa tggcaaatct
     3001 gctacttctt tttcttttaa taattcatgc ccacgttcta gataactatt tatttcttgt
     3061 tcatcttctg taaaagctgt cattgtttcg gtagggatgg ctttggctga atgcttaagg
     3121 atttttttct cttcatcgat ggttaaatgg acttcaccga catactgccc gtactttcca
     3181 gcggctgcta tttggacatc attgattacg tgaccttctc tgaataaatg atgggtgtgc
     3241 gatcctaaaa taacatcaat tgctggtatt tcttttgcaa tttggaaatc atcctgaatc
     3301 ccgagatgac tcatcaaaac taaaacatcc acttgtgaac gtaaatcttc gactaattct
     3361 ggcaaaatat cataaggatt ccgtatgtcc cagccattcg gattataagt aagcggaaat
     3421 ggagcagtaa atgcaattag tccgattttc gtctggtgtt tcgttgtaat gattttatat
     3481 ggtttcgtcc atgccggtgg ctggagcgta tttttatcga ataagttatc caggatgata
     3541 tcaaactcag catgatcgta taaatgattc aactcttctt tggaatttcc tacgccttca
     3601 ttgtttccta tcgtcgctgc atcataacca acttcattca tcagcaagat attttcctgt
     3661 ccattcgttg cttctgacaa tggatgccaa cgatccacaa aatcacctag atctactgca
     3721 atcgttgtcg ttttttcttt cttttcgttt tctcgtttac gttgatccaa aaaccttctg
     3781 atccttggcc aattttctaa atgagaatgc agatcattcg tatgtaaaat cactattttt
     3841 tccatgtacc ctctcgcctc tctttatctt tatcatacca taggcagctt tttcttccat
     3901 tgaaacaagc tctatttcag agtttttcct gccttgaagc aaattgtata agtacaatat
     3961 tcgataaatc agtctggcta aaaaatcagg aacattcatt tttacagtac tataaaaata
     4021 aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa tcagacacat ggcggaacct
     4081 gcttagtgct gcttccttcc agacctgaca cgcttcacaa gcccaccatt gtcctagcct
     4141 ttcggcaaag aatagtatac cttatttttc attcgttgac cagtcttttt ttaatttctt
     4201 ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc atcccacatt cgtcttctaa
     4261 aatgccacct tctacatatg cttggtggtt aaaccgctcg tcctccaaaa gattcatcaa
     4321 ggtaccggct gttccgcctt ttggatcata cgcgccgaaa taaacttctg ctacacgaga
     4381 caaaatcatc gcgccgctgc acataggaca aggctctaag gtaacaaaaa gtgcagcatc
     4441 ttccaagcgc cagcttccca ctttttcaca ggcttccttg attgcaagca tttctgcatg
     4501 tgtagtagca tcttgcgtgg tttctctaag gttatatcct cttccaatca cttttccatc
     4561 caatacaacc actgctccaa tcggaacttc atgaagcatt tctgctttct ttgcttctgc
     4621 caatgcaagg cgcatccatt tttctttttc tactgcatca agatttgtat ttaccaatta
     4681 cccttccctg cctttgtttt ttcttgtttt actttagcat atgataaaaa ggctgtaact
     4741 aacttttttt tctatggtaa aataaataca agaggtgaac aagatgaaaa tggataaatt
     4801 acttgctctt tacccacaag cacagaaaaa aaactctcca gcatcagaag cggatatcct
     4861 ttcttttgca attgatgatg cttttatctg gatcaaacaa gattcattat ctcaacaaga
     4921 aaccagcctt ttgaaagcgt tattccctgt aatcaacgat caaaaaaaac acccatggta
     4981 tcactatctt tttaaagatg cttcttgtgt tcaagaaaaa agctttcgtt tgatccagct
     5041 tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg caagatacta ttttggaatt
     5101 atttcgctat tctgctgatt tcttttttta tacagacaca gatgcgctct tagtcgaaca
     5161 aataaaccgt gattatcttg atgctactga aatcaacggt atttttctat ctttagatgc
     5221 tgattttgat acgacaacgt ctgtattcgt tggttctttc cattcacctg gcaccgacct
     5281 cgtccctttg tttgctgaag aaagaaaaat ttttttaaca gagcaaaaca atctttatac
     5341 tcacagccgt actttttcta tgcaagatgt ggctttacat tactatacaa aagatactgt
     5401 aaaagatagt aagttgatca aaagttacat agaactgatc aaacaaaatg aaatggaagt
     5461 gatcattcaa gaattatgga atgaattagg caatgtcagc tctgctgcaa aaacactgtt
     5521 tcttcatcgt aatactctaa aatatcgaat cgaaaaattt caagaacagt ctggtttcaa
     5581 tctaaaaaaa gccaacgatt tactgttttg ctatctttta ttgcttcatg aacataatta
     5641 aagatagatt aaaggggttg tgacaataat tttgtcacaa ccccttattt cgaatgaata
     5701 gtgttcaaaa gctagtttct cttacatcat tttttcaaca aaatgcatta gccggtcttt
     5761 tacatcttcg gttgaatctt tgattgaatc accataaaca gagattttct tttttcctga
     5821 tttgattttt gctaacattc ccatcaaaga atccaagtca ctatctgaaa gaccatctac
     5881 gatttctagt aatttttcat ttccatcaaa tttgtcatag acgaattttt ttactttcgt
     5941 ccgagtagtc atatgatgga tcttttccat tacttttccc gatacaacga ctgctgtcgc
     6001 tacactggca gcggctgcta cgcttaatcc gattgttact tttgttgatg ttttcataag
     6061 tatcagctcc tttttatcta tctccatcat agcatagaca aaaaaataaa cgaactaatc
     6121 cactcagtga aatctcaatc ctttagggaa aaagttcttt actgttcctt ttacttgttt
     6181 cccaaatccg acaggtatct tatctaatac tagcaaaacc catccttgat tcccatcacg
     6241 ttgaaatgtt tctcctgctg tgtaagattg ccattccttt tgagtgatcg gcaagcaagg
     6301 tatattctct atcttttttg tagcaagcgc taatgcatag ctaggttcaa atcgattctt
     6361 tttgaaatct cctaaatgca aacctgtacg tacgactttt aaaccatcga gagaaggggc
     6421 taactcagga acttcccaaa gatggtcgtt gaacactaaa agtcttccag tagcttcata
     6481 atgaaaatca tttgaaaact ccgtccagag tttttcctgc tcttttgtca tttgcacttt
     6541 actttttttt cgtgtcttct tttctttatg catctgattt tgtccatgaa aagtcagctt
     6601 tgcaacaaaa tgtccttcac cctgatcttt atgaggccat atacgtattg ttttttccaa
     6661 accagctacg cttccccatt cagaacgtcc tgaactaacg gattgagtca aaggaatctc
     6721 ctcaatcgtt actggataat tttctacaag ccagctaatg atctcttcat tttcttctgg
     6781 tgcaaacgta caagtcgaat aaatcagttg tcctttattt tttagcattt ttattgctga
     6841 agacaaaatc tcttgctgac gtttttgaca gtatagcgga gattcttctg tccactcttt
     6901 tattgcattt ggatcttttc ggaacattcc ttctcctgaa caaggagcat caaccacgat
     6961 ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg ggtgcatgat ttgtcactat
     7021 tgcgttcgaa actccccatc gttctatatt ttctgataaa atcttcgcac gtttcggaaa
     7081 gatctcattc gtcactaata accctttccc cttcatttga gcagctaatt gggttgattt
     7141 accaccggga gccgcacaca aatccaaaac tttttcaccg ggttttgctg cagcagctgt
     7201 tccaacgatc attgcacttg gttcttggct atattcatac ccagcttgat ggaggaaaga
     7261 ctttccattg acagtgccaa gaaatccctc attgctgtag ggagcgggtt gtaattcttc
     7321 gctatggtaa gtttggacca tgtccaatcc tgccggtttt agtggattcc accgaaatcc
     7381 ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg gatgcttctt cgcctaaaag
     7441 aagacgatat tttttgataa attgctgtgg taacgttgtc gcttcctcct tcattccaat
     7501 gtttcgttag acattttaca atctttttta cgaaaaataa aggtggctga tctacttttt
     7561 catgttgctg cctaaggtga ctgttccttt aagatatttt ccaagtattc atccgtcacc
     7621 tcgtttttat cccaaacagc gttcatcatt tcaagaaaat cttgagaaga atcatctgag
     7681 cgcaaacttc ccaatgcatt aagccatacc aattcaagcg acttttcttc tttgatcttc
     7741 gtgtgacctt tgacatatga gaaaaatggc cgcttaaata gcgaatccat gtctaaatca
     7801 gattttgcaa tgatttcacg aatactttca tggatagaaa aaaaatcttt caataagtag
     7861 acaatgctta aaacatcatc ggaactgcat tcagctaagt agttttcaaa ataaacagtg
     7921 atttcttcta atatcttctc ttttaacccg ctgattgatt tgaaatgtgt ataaatcggg
     7981 tatgtagaac aaccgcagaa ctctgctaac tctcttgcag taattgactt cattccatat
     8041 tcttccacgt ataccatact tttgaacagc aaatattctt tactataatt tgttactctc
     8101 cccatcattc ttacctccgt ctcttttcat ctacccataa ataaataaca taaataattc
     8161 taaaaatatt att
//
LOCUS       DACEOE010000106         8144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEOE010000106 DACEOE010000000
VERSION     DACEOE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.108"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..1392
                     /locus_tag="IW774_001960"
     CDS             100..1392
                     /locus_tag="IW774_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1154301.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(1535..2329)
                     /locus_tag="IW774_001961"
                     /note="aph(3')-IIIa"
     CDS             complement(1535..2329)
                     /locus_tag="IW774_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1154302.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(2422..2964)
                     /gene="sat4"
                     /locus_tag="IW774_001962"
     CDS             complement(2422..2964)
                     /gene="sat4"
                     /locus_tag="IW774_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1154303.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            complement(2961..3873)
                     /gene="ant(6)"
                     /locus_tag="IW774_001963"
                     /pseudo
     CDS             complement(2961..3873)
                     /gene="ant(6)"
                     /locus_tag="IW774_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            complement(3906..4640)
                     /locus_tag="IW774_001964"
     CDS             complement(3906..4640)
                     /locus_tag="IW774_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1154304.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(4621..5490)
                     /locus_tag="IW774_001965"
     CDS             complement(4621..5490)
                     /locus_tag="IW774_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1154305.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            complement(5593..5838)
                     /locus_tag="IW774_001966"
     CDS             complement(5593..5838)
                     /locus_tag="IW774_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1154306.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            complement(5874..6539)
                     /locus_tag="IW774_001967"
                     /pseudo
     CDS             complement(5874..6539)
                     /locus_tag="IW774_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            complement(6702..6962)
                     /locus_tag="IW774_001968"
                     /pseudo
     CDS             complement(6702..6962)
                     /locus_tag="IW774_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            complement(7132..7869)
                     /gene="erm(B)"
                     /locus_tag="IW774_001969"
     CDS             complement(7132..7869)
                     /gene="erm(B)"
                     /locus_tag="IW774_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1154307.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(7994..8077)
                     /locus_tag="IW774_001970"
     CDS             complement(7994..8077)
                     /locus_tag="IW774_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1154308.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         2414 a         1696 c         1267 g         2767 t
ORIGIN      
        1 tgacaccagc atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta
       61 gaattaattt ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc
      121 aaagaaacat tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa
      181 gaaaaataca aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa
      241 gaatgcatcc actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc
      301 gtccggttgt taatgaacga tgtttctgaa taccgtacct acctcgaatt gaaaaagcaa
      361 cgcttcaaat gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa
      421 cattgtttca tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc
      481 tcaatgacag agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg
      541 aagcgatttt atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt
      601 gatgaattca aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga
      661 caaacacagc gcttattgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat
      721 tttagtcatt tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac
      781 gctccttatg tatcgctcgt taaaaagctg tttccaaaag cacagctgat tattgaccgg
      841 tttcacatcg tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac
      901 caactattaa aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac
      961 tggaaacttc ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct
     1021 ggcttcaaag ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct
     1081 gctttgcgtg taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac
     1141 tacgtatcgt ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg
     1201 accttgagaa cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat
     1261 tcaaatggcc cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgcatat
     1321 ggctttcgta gcttttataa tttcaaattg cgcatcatga ttcgtcatgg caacgcattg
     1381 attttcaact aaaaaagatc aagaggaact gatccacttg atctccaatt attctaagat
     1441 ttctctttac caacatcatt tgacaaagag ccagaaaaga ttagatgtct aaaaagcttg
     1501 tagttaaagc tttttagaca tctaaatcta ggtactaaaa caattcatcc agtaaaatat
     1561 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
     1621 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
     1681 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
     1741 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
     1801 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
     1861 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aatcggctgt
     1921 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
     1981 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
     2041 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
     2101 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
     2161 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
     2221 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
     2281 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
     2341 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
     2401 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
     2461 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cacaattatg
     2521 atagaattta caagctataa ggttattgtc ctgggtttca agcattagtc catgcaagtt
     2581 tttatgcttt gcccattcta tagatatatt gataagcgcg ctgcctatgc cttgccccct
     2641 gaaatcctta catacggcga tatcttctat ataagcgtac cggttccaat tttttcgcag
     2701 tttaactttt ccgacgcatt tatcgtcttg gtagtaaaga tatattatct tatcagtatt
     2761 gtcaatatat tcaaggcaat ctgcctcctc atcctcttca tcctcttcgt cttggtagct
     2821 ttttaaatat ggcgcttcat agagtaattc tgtaaaggtc caattctcgt tttcatacct
     2881 cggtataatc ttacctatca cctcaaatgg ttcgctgggt ttatcgatat ctttcaggtg
     2941 ccctgctttc atttctgtaa tcactgttcc cgcctctctt ctatatcagc ggcatatgtg
     3001 ctatccaggc agccggtttt accagtgtat tttttataca tgtccctggt atattttgtt
     3061 atattcctat catactccgg ataggcataa tgaagccttt ccgccacctc accggatacc
     3121 gccctgaaca attgatggca tagaaataat gcttcccata tgttttcata ggaatccatc
     3181 cggtaggtgg acaaaagttt ctcccacaaa tcctcggata tatacctttc aataaactta
     3241 tagttcttgc ctacacttaa tttaaagcct gtttcgatgc cgaccttcca tgatatcatt
     3301 ctcagcagct catggcgaac aatctgatta aaatgatcaa tagcaaataa aatttcctta
     3361 cggcacaatc ctttaataac ataaggtgtt acattccaaa attcattgca gcaatcatca
     3421 tactcccttg cgcttggctt tcttacatga taatctatat cagtcggaac tatgtccctt
     3481 ttaattctac aatctttatc aattagaacc tttattaatt tatcgccctt taggtaatta
     3541 tctaactctt ccaagggcaa taaggtaaga tcaattttat tgtaatcatc aaatagcata
     3601 agataggaaa atcccttttc ttcaggtggg aataattcca tatcctccgg cttttgcatc
     3661 attattatat tcccaaattg attaagccag tcatcattag atataaacgg ttctatatca
     3721 cttacaaaat atgtaatatc ataatcctga aattcatctt taggtatatt aatatttgcg
     3781 cgtgacccct caagggtcac aattcgaata cgttcgttca tcctgttctg ctaaagaaag
     3841 tactaaatcc atcatttctt tttctgatct catttacata ctcctcgttt attttttcta
     3901 tattattaca tcttcttttt tgccgataca atcagcatca ttgggcgtcg catttcatcc
     3961 gccatccccg gaatatccat catgttctct ggcggctgtg gctccacaat ctgatttatt
     4021 ataaaactat ttgaaagcag tgtatttaga tatgtggtca gtgttctatg atattttgta
     4081 accttttctt ccaaaaacat agctgtccgt ttgccctcat aataataatt gtccaccggg
     4141 aaatgcagta tttctccttt ttcgttataa taccagtctt gtgttccatg agcagtaaaa
     4201 acaggatgtt caactgtaaa aactaaattg ccaccagcct tcagcatcct atatatcttt
     4261 tttattaaat tctcatagtc tgctacataa tgaaacgcaa gcgaacttag tattacatca
     4321 aagctctcct ctgggaaatc cacatcttct atggcacagc attcatattc aatctgtgga
     4381 aaatgggttt ttccttttgc tacttcgagc attttatgag aaatatcaac acctactaca
     4441 gaggaagcac cgttttccat cgcatatata cagtgccatc catagccgca tcctaaatca
     4501 agcacacgct tacccttaaa atcaggtagc atctttttca aagtctccca ttctcccgca
     4561 ccagccagtc ctttctgcga gcgactcatt tgactgtatt tttgaaaaaa tatattatca
     4621 tcatatttgt tttctttcat ctgaactctc ctcgtttaaa aagttattta tctccgatac
     4681 aatttcattc acttcattat aaagcttctc ggtcatgtcg tagcattcaa aaagtgagat
     4741 accgagtact tcaaaaatat gatttacctt ctcggtatat ttttcaggtt tatgttcaaa
     4801 agtttcaagc agttttatag ctttcttttc gttgatacaa taagcattat tacatgcaaa
     4861 taacacttga tttaaacatg aaactatacg aaaaacatga cccgcaatat aatatttatc
     4921 gtctgttccc gaatttgctt ttacaaacat taaagagaac cctgcttcaa acataaaaaa
     4981 gttaactaaa cttttctgca aagcattggg ataagtttct gcctgttttt ttaattcgca
     5041 taagctttca ttcttagcat atagtatttt gctaatcgct aattctcctc gatacattgc
     5101 actaatataa ccatggggat gcccagtctg ataattggca gtaacaattc cgtgctctgt
     5161 atctttcatt atttgttcca cacgtttaat atcacgtaaa attaaatcca catgataccc
     5221 gtttatgact aaccatccgc cgccattaat ccaatcaccc catgctccgg gaggtacaac
     5281 aaggttattt ctatgctcat catccagctt tgtagcgaat tgattaatag tatttatgtc
     5341 aaatgattct gaattgtaat agatgccgat atctatatcc gaatcctctg tatgggtgcc
     5401 ccttgcacgt gaaccaccta aaacaatacc ttctatataa gacagagagg ataatttctc
     5461 tgctactgat ttaataatat tatctaccat caagcatgct ccttttatcg attcactgct
     5521 gtaaaaattc caagccaaat cttttgcttc tactcaacat atttataatt tttttaattt
     5581 cacaaaagat agttaaaact tatcaattgt ctttttatac ctctgctgat tagatgaagt
     5641 cttttcaata gcctttaaat taccattcat tttgctaaca tggtttaaaa ataacgttcc
     5701 aagcagaagt cgatcctttc tgggtatcct attccccaca tatcccttga ataaatcttt
     5761 aacaagaaaa acttcgccat catttagatt ttcagtttct ctaatagctt cttccaatag
     5821 ctcatttaca tcaatcatgt tgtccactcc ttattaataa tgggtcagcc cccttctact
     5881 gtttcggttt ggtcaatcgc ttcaattaat tgattttcct tttcttccaa agattgacga
     5941 atcctttcaa tctcttctat agaaagttca tcctttaaat catctattaa ctcaggtaag
     6001 atttttgttt ccaccatttg ttggtagttt tcaagggctt tttgatcaag gctatcctta
     6061 tactttaaaa tacttttttt ccaaacgaaa gagtatttat tcgcatccgc ttcaattttt
     6121 gtaccatcaa tgtatagagc actgccactg attaagccac tttgaaccag tagctcacga
     6181 aattgaacaa atgaatgatc tagaatatcg ttcataatcg gatgaacgcg aaaacgatta
     6241 atagtacgaa agttagggaa ttgctcttga gataagtagc gcatccgaat actatctatc
     6301 agcatatttt gtatttggcg gcccgaataa accccttgaa tataggcaca aagaataatg
     6361 gaaagtagca tacgaggatg atattggggt ctacccatag attgataata aggaagaaag
     6421 acttcatcgg gaatagagtc aacaaactgc acaatggcat gagcaaaatc attttcttct
     6481 aatttaattt caagatctat aggcaaacaa agttggttta tggtataatt ttgatacatg
     6541 ataaagcacc tctctcaaat ggattaatca cctatattat agcagagtgt gctttatttt
     6601 tgtattcact ttcaaaagat aaatagaggt ctttccacta ctatctaaat ggaaagacct
     6661 ctatttttca tataggagct acttttgggt cagccccatg tttattttga aaaagagata
     6721 aaatcaatat atcccttttc cccaattttt acaacggcat tgtagggctt tttctctttg
     6781 tttttgattc cttttaccag ggtttctttt ccctccagta attcttttac attcgttttg
     6841 gttagttttt tctttctaaa atgttcagct aaagtaaact tacattcagg ataatttgaa
     6901 caaccataaa acgatttttt taatacaata ttgttgccac acttaggaca ttttcctaca
     6961 aggggtcccg agcgcttagt gggaatttgt accccttatc gatacaaatt ccccgtaggc
     7021 gctagggacc tctttagctt cttggaagct gtcagtagta tatctaataa tttatctcca
     7081 ttccctttag taacgtgtaa ctttccaaat ttaaaaaagc gactcataga attatttcct
     7141 cccgttaaat aatagataac tattaaaaat agacaatact tgctcataag taacggtact
     7201 taaattgttt actttggcgt gtttcattgc ttgatgaaac tgatttttag taaacagttg
     7261 acgatattct cgattgaccc attttgaaac aaagtacgta tatagcttcc aatatttatc
     7321 tggaacatct gtggtatggc gggtaagttt tattaagaca ctgtttactt ttggtttagg
     7381 atgaaagcat tccgctggca gcttaagcaa ttgctgaatc gagacttgag tgtgcaagag
     7441 caaccctagt gttcggtgaa tatccaaggt acgcttgtag aatccttctt caacaatcag
     7501 atagatgtca gacgcatggc tttcaaaaac cactttttta ataatttgtg tgcttaaatg
     7561 gtaaggaata ctcccaacaa ttttatacct ctgtttgtta gggaattgaa actgtagaat
     7621 atcttggtga attaaagtga cacgaatgtt cagttttaat ttttctgacg ataagttgaa
     7681 tagatgactg tctaattcaa tagacgttac ctgtttactt attttagcca gtttcgtcgt
     7741 taaatgccct ttacctgttc caatttcgta aacggtatcg gtttctttta aattcaattg
     7801 ttttattatt tggttgagta ctttttcact cgttaaaaag ttttgagaat attttatatt
     7861 tttgttcatg taatcactcc tgaagttatt acatctgtaa ataaatacag aagttaaacg
     7921 atttgtttgt aattttagtt atctgtttaa aaagtcataa gattagtcac tggtaggaat
     7981 taatctaacg tatttattta tctgcgtaat cactgttttt agtctgtttc aaaacagtag
     8041 atgttttatc tacattacgc atttggaata ccaacatgac gaatccctcc ttcttaatta
     8101 caaattttta gcatctaatt taacttcaat tcctattata caca
//
LOCUS       DACEOE010000107         8139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEOE010000107 DACEOE010000000
VERSION     DACEOE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.109"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            67..1260
                     /locus_tag="IW774_001971"
     CDS             67..1260
                     /locus_tag="IW774_001971"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1154309.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1390..2862
                     /locus_tag="IW774_001972"
     CDS             1390..2862
                     /locus_tag="IW774_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1154310.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            2945..3733
                     /locus_tag="IW774_001973"
     CDS             2945..3733
                     /locus_tag="IW774_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1154311.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3800..4468)
                     /locus_tag="IW774_001974"
     CDS             complement(3800..4468)
                     /locus_tag="IW774_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1154312.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4583..6295
                     /locus_tag="IW774_001975"
     CDS             4583..6295
                     /locus_tag="IW774_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154313.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6298..8070
                     /locus_tag="IW774_001976"
     CDS             6298..8070
                     /locus_tag="IW774_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154314.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2486 a         1371 c         1797 g         2485 t
ORIGIN      
        1 gttcccgaaa ggatccgcag gacggagatg ccttgtaacc gaatttaact agggggaatc
       61 attataatgg tagaaagaca cttatttaca tcagaatctg tttctgaagg acatccagat
      121 aaaattgctg accaaatcag tgatgcaatc ttggatgcaa ttttaaaaca agatccaaca
      181 gcacgagtag cgtgtgaaac gtctgtaaca actggtcttg ttttagtatt tggagaaata
      241 tctacgacgg catatgtaga tattcaaaaa gtcgtacggg aaacaataaa agaaattggt
      301 tatacacgtg caaaattcgg atttgatgga gatacagcag ctgtattggt tgcgattgat
      361 gaacaatctc ctgatattgc tcaaggagtt gatgaagcac ttgagatccg ggatgaagat
      421 aaaaaagacg tattagatga gattggtgct ggtgaccaag gtttaatgtt tgggtttgct
      481 gtagatgaaa caccagaatt gatgccttta ccaatcgctt tgagtcatcg tctggtacga
      541 cgcttggcag atttgcgtaa atcaaatgaa ttaacgtatt tacgtccaga tgcaaaatct
      601 caagtaacgg ttgaatatga tgatcaagga caaccggaac gcgtagatac aatcgttatt
      661 tcaacacagc atgatgatgc agtagataat gaaacaattc gtcatgatgt catcgaaaaa
      721 gtagtgaagg aagttattcc agctgaatta ttagatgatc aaacgaaata ttatatcaat
      781 ccgactggcc gatttgtcat tggtggtcct caaggggatg ccggattaac aggaagaaaa
      841 atcattgttg atacgtatgg cggttatgct cgtcatggtg gcggtgcttt ttctggtaaa
      901 gatgcgacaa aagttgaccg ttctgccagc tatgctgcac gttatattgc taaaaacatt
      961 gttgcagcag ggcttgctcg caaagcagaa gtacagctag cttatgcgat tggtgttgct
     1021 caacctgttt cgatctcaat caatactttc ggtactggaa cggttccgga agaagaactg
     1081 attgctgcag tgagagaaaa cttcgatctt cggccagcag gaatcatcga gatgcttgat
     1141 ttgcgtcgtc cgatttacaa acaaacagct gcttacggtc attttggtcg tacagatgta
     1201 gatttgcctt gggaacatac ggacaaagta gatgcgttga aagcaagttt agcaaaataa
     1261 gttatattca gctggaacga tcgccaagtg gtggttgttc cagctttttt tatggcagtt
     1321 aacctcttga aaaagatagt aattatttat ctatttttag atcctgtcat aatgaaaggg
     1381 aggaataaga tgaaaaaaga aacaaatgtt actttggtca cagtgagtat tttcattgca
     1441 acgttcatga cggctattga aggtacaatc gtaacaaccg ctatgccaac gattgtcggt
     1501 tcattacatg gaatggaaat catgaactgg gtattttcga tttatttgct gacaaacgct
     1561 atgcttactc cgatttacgg aaaacttgcg gataaagtgg gacggaaacc aatctttatt
     1621 tttgggacat tattgtttat tatcggttcg ttattttgcg gtttatctca aagcatgatt
     1681 gggttgattg ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat gccagtagct
     1741 ttgacgatca tcggcgatct ttattctatt gaaaagcgag caaaagtttt aggtttgaat
     1801 agttcagcct gggggattgc cagtgttttt ggaccgcttg cgggaggatt catcgttgaa
     1861 acgatcagct ggcactggat cttttttatc aatgtgccga tcggaattct attaattatt
     1921 ttgatttggc tatatttaaa tgaaccaaaa atcatacatg agtctaaacc aatggatatt
     1981 tttggtagtt ttagtttgat ggccatgctt gtctcattgc tgttagggtt ccaattatta
     2041 ggagacgaag gaatgagtcc gcttgtttct gctttgctgc taggtagtat cgtaatgtta
     2101 ggtatttttg tttttgccga aaaaagagct aaagatcctg tcatcatgat gcatttattc
     2161 aaaaatccac tatttgttgt ggtcaacttg gctgcagcat tggctagtgg ttttttgatg
     2221 ggaatcgatg tttatatccc tatgtggatg caaggtgtat tagggctgaa tgcagcaatt
     2281 ggtgggctgg tattagcacc attatcacta gtctggatgg tcggttcttt ccttgcaagc
     2341 aagtggatga ctcaaatgaa aactcattgg gtcttgagaa ttggactact aatcgcagct
     2401 attgggggcg cttggttgta tcttatgcct ttcagaagta actatttatg gttttttgct
     2461 atatcaacgg tcatcggtat aggcttaggt gtgaccatga ctacctcgac tgtcagtgca
     2521 caaaatagtg tatctcgtga ccagcttggt gtagcaactt cttttaatac gttagttcga
     2581 acaattggtc aaacagtcat ggtttctatt tttggtttat tgataaatgg ggtaactaaa
     2641 cgagaacttg cagcagcaaa tttaacaaat gatgcagata tcatgaataa actggtgaat
     2701 ccacaaacgg ctaaatatat tgatgcggat attttgttac cattaagaaa aattctttat
     2761 gatgggttac attccgttta tctagttggc ttgttactcg tgatcattgc tttggtctta
     2821 acgcttttcg ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt aagatatgta
     2881 cgtagccgaa tcattcaaat ttaaagttat tcaaaaataa cttcttacta aggaaggcga
     2941 aaatatggca tttattcaag caaatattta ttcgaatgtg ttagaaatgg aagtcaatct
     3001 aaatgtgata ctaccgcaaa caacaaaaaa ggtgattggc accagttcag agcaagagac
     3061 aacggatata cctgttttat atttacttca tggaatgggt ggcaatcata gtgtatggga
     3121 aagacgtacg tcgattgaac gttatgtagc aaactacgga ttagctgtca tcatgccatc
     3181 tactgatctt ggctggtaca cagatacagt ttatgacatg aagtattgga cattcattgc
     3241 agaagagcta cctgagattt gtcatgaatt attcccgcaa ttaacaagaa aaagagaaaa
     3301 aacatatgcc gctggattat cgatgggcgg atatggcgcg ttaaaactag gccttgcaaa
     3361 atcagaaaat ttcgcagcag taatctctct ttcaggcgct gtttctgtag gaaatcgaat
     3421 ggatgatctt ttgatggttc gtaaagcatc tttttgggaa gggatctttg gaccattgga
     3481 tcaaatcgaa ggttcaaaaa atgatccgca atacttattg aaagaactag taaaaagcgg
     3541 aaaaccagtt ccaagatttt atatggcttg tggggaatct gattttcttt atcaggcaaa
     3601 tcaggaaatg gtatctgcta tgcaggaaca aggcttggcg gtcacctttg aacaaggacc
     3661 tggcgatcat aactggatct tctgggacca atggattcaa cgtgcattgg cgtggctaat
     3721 ggaagaaaaa taaaaaaaga gtcttaaaaa gatagctcct atagagatca caaacatagt
     3781 gatctctata ggagcttttt tactgctcgt tttctcttct tttattttgt tggcataagc
     3841 cttcaatata ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat tctgcatggg
     3901 actgcatact ttctttaaat ccctctaatt gattttcaac tacataatta cgcataaata
     3961 gaactgtttc tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc tgctgttcat
     4021 gctgctgatc cattaactgt ccaatcaata aattgtggag agaggcaatg gcctgatgac
     4081 tttgggtgac tgaaaatcct tcacgaacaa gtaattggat catttgctcc aaatgttcta
     4141 atcgactcgg aaaagcgggc acagtattga tttctaattc tgccccacaa gggaaacgtg
     4201 tataaaggtc atagtaattt tccatatact gcagcaactg ttcattccaa ggcaaagttg
     4261 gatctggaag cgctaattct tcttccattg tttcagccat tttttgcaag agctgttgtt
     4321 tgtttttgaa ataccaatat aaagcaggtg cttgaacatg aattctgttt gcgatgtttc
     4381 gcatggaaag ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg gcctgaatga
     4441 tgattgcttg agaaagcttt atttccattt tatcaacctc gtgtattgca ttatcttttt
     4501 aaacatggta aattgttagt cggtctaatt ttacagtgtt aaattagtcc tgtcaattta
     4561 aacaaagggg tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa aatataaagt
     4621 agctgttttt atttccttgc tgtccgtagc agtcatggtt gcagcagcat tatggcaacc
     4681 taagttacta cagcaagtat tggaagctat tattacagaa gataatgatg aaatgaaaac
     4741 aattggtatt tacctgatca gtctggcttt gctaggatta gccgcaggtg taacgaacac
     4801 gattttctct gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag aagcatttcg
     4861 aaagatccag acattttcat ttggaaatat cgaacagttc tctgctggaa atcttgttgt
     4921 tcgtctaaca aatgatatca cgcaaatcca aaatttagtg atgatttctt tgcaatcact
     4981 ttttcgaatt ccattcttat ttattggtgc cttcatttta gctatgatca cgatgccgca
     5041 attatggtgg atcatcgtag cgcttatcgt tacggtattt ctgattacgg cactttcatt
     5101 tacaaggatg ggcaagcact tcatgatcat tcaaaaactg attgatcgtg tgaatagcat
     5161 tgcaaaagaa aacttactag ggattcgtgt agtgaaatct tttgtacaag aagaaaacca
     5221 attgaagtca ttcagcaaag taagtgagga cttaacaaaa cacaacatca tagtcggtac
     5281 attattttct gtgatgatcc cgtccttcat gctggctgcc aatttggctg tggttggggc
     5341 gatctttttt gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg gaattgcttc
     5401 attcatgaac tacttgatgc agattatgat ggcaatcatt attggcggaa tgatgatgat
     5461 gatgacttca agagcagccg tatcgctgaa gcggattgcg gaaattttag atgctgaacc
     5521 agatcttact tatttagacg ttccagaaca agagttgacg ggttctgtca cttttgacca
     5581 tgtttctttt cgttatcctg gtgaggatac agatacgctt aaagatatca gcttttctat
     5641 caaacctgga gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt caacactagc
     5701 acaattgatc cctcgtctat ttgatccgac agaaggcaaa gttgaagtag gtggtgtaga
     5761 tcttcgtcaa gtaaatgaaa agagtttaag acaaacggtt tcctttgttt tgcaaaaagc
     5821 aattttattc tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga atgcaacaga
     5881 aaaagagatg gaacatgctt cttctattgc tcaagcaaaa gaattcatcg aaaagttatc
     5941 tgatcgttac gaagcaccaa tagaagaacg tagcagtaac ttttctggtg gtcaaaagca
     6001 acgtttatcc attactcgtg gcgttatcgg taatccaaag atcttgatcc tagatgacag
     6061 tacgagtgca ctggatgctc gctctgaaaa gctggtccga gaagcactcg atcgagattt
     6121 gaagggaaca acgacgattg tcatcgcaca aaaaattgct tctgttgtca aagcagaccg
     6181 catcttagta ttggatgaag gcagattagt cggcgaagga acccatgagg aattggtagc
     6241 tggaaaccgt gtctatcagg aaatctttga aactcaaaaa ggaacggagg agtaagtatg
     6301 actgatctaa taaaagcaag taaatttttc tatcactatt tgaagcggta caaactctcc
     6361 tttttcttta tctttatcac agtgatcatt gctacctatc tacaagtaaa ggcacctcaa
     6421 tacatcgggg aagcttttca agaattagct aattacgtag gaggcttgat gcaaggcgta
     6481 gacgataaaa gcaaatttgt ttctgtaata tggaaactct tgctgtttta tgtactagct
     6541 agtgcagcca actttatcta tagtattttg ttcacacaag tagtagggaa gtcaacgaac
     6601 cgaatgcgta tcggtctatt caacaaattg gaaaaattga cgatccgttt ctttgattct
     6661 catcaagacg gtgaaatttt aagtcgtttt accagtgact tagataatat tcaaaatagt
     6721 ttgaaccaag ctttgcttca agtcatcacg aatgctgttt tacttgtcgg gatcttgatc
     6781 atgatgttcc gccaaaatgt agagttagct tgggctacaa ttgcttccac tccagtagcc
     6841 gttttgatag ctgtcttgat tatccgcaaa gcacggaaat atgtggatct tcagcaagat
     6901 gaagtgggga aactaaatgg ttatatggat gaaaaaatca gcggacaacg tgtcatcatc
     6961 acaaatggct tacaagaaga aacgattgaa ggattcttag agcataatca aagtgttcgc
     7021 gaagcaacct tcaaaggaca agcttattct ggtctactat ttccgatgat gcaaggaatg
     7081 tcactggtca atacagcgat agttatcttt ttcggcggct ggttagcatt aaatggtgat
     7141 ttggaacgtt cggtggcatt aggcttagtt gtgacattcg tccagtattc acaacaatat
     7201 taccaaccac tgatgcagat ttcatctgga tacagcatga tccaattagc gattacaggg
     7261 gcaagacgat tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga aaacggtaaa
     7321 gcatttgatg gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg ttattctcca
     7381 gaaaaactag tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat ggttgctctt
     7441 gttggtccaa caggatcggg gaaaacgacg atcatgaatc tgctaaaccg tttttacgat
     7501 gtcaatgatg gttcagtagt catcgatgga atagatatcc gagattatga tcttgatgca
     7561 ttacgtagac atgtaggaat cgttttgcaa gaatctgtat tgttctccgg aacgatcaaa
     7621 gacaatatca cattcggcaa accggacgct tcagatgaag aagtcatcaa tgcagcaaaa
     7681 caagcgaata ttcatgactt catcatgacg ttagaaaaag gttatgatac agagatatcc
     7741 gaagaaaata acttattcag tacaggtcaa aagcagttga tcagtattgc tcgaacgatc
     7801 atcacaaatc catctttatt gattttggat gaagcaacaa gtaatgtaga cacggtaaca
     7861 gaagcaagga tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag ctttgtgatt
     7921 gctcaccggc tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg tgatggggaa
     7981 gtcattgaag aaggcagtca tcatgagtta ttagcagaaa atggatttta tgcggaactt
     8041 tatcataatc aatttgtatt tgaaagttaa attaaaagaa cctcgttata acggggttct
     8101 ttttatatat tttatgttgt ttgtttttat aaggggcag
//
LOCUS       DACEOE010000108         8130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEOE010000108 DACEOE010000000
VERSION     DACEOE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.110"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(16..834)
                     /gene="purR"
                     /locus_tag="IW774_001977"
     CDS             complement(16..834)
                     /gene="purR"
                     /locus_tag="IW774_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1154315.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(1001..1825)
                     /locus_tag="IW774_001978"
     CDS             complement(1001..1825)
                     /locus_tag="IW774_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1154316.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(1798..2478)
                     /locus_tag="IW774_001979"
     CDS             complement(1798..2478)
                     /locus_tag="IW774_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154317.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(2501..3439)
                     /locus_tag="IW774_001980"
     CDS             complement(2501..3439)
                     /locus_tag="IW774_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1154318.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(3684..4527)
                     /locus_tag="IW774_001981"
                     /pseudo
     CDS             complement(3684..4527)
                     /locus_tag="IW774_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
     gene            complement(4681..7254)
                     /gene="mprF"
                     /locus_tag="IW774_001982"
     CDS             complement(4681..7254)
                     /gene="mprF"
                     /locus_tag="IW774_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1154319.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            7395..>8130
                     /gene="proC"
                     /locus_tag="IW774_001983"
     CDS             7395..>8130
                     /gene="proC"
                     /locus_tag="IW774_001983"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1154320.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAG"
BASE COUNT         2469 a         1782 c         1389 g         2490 t
ORIGIN      
        1 ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa tatttttcgt
       61 ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg cgcgatgacc
      121 gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga attcttcaat
      181 caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca ctacaaggac
      241 acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc tttcagaaga
      301 tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt cccggcgtac
      361 aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta cgccttttgt
      421 ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa tgattcttcc
      481 aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat atcctcctgg
      541 aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt ttttcgcctc
      601 ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga ttgtttccaa
      661 ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt cactgataga
      721 agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt catgcgggtg
      781 attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt tcacctattc
      841 caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc aaaaattcga
      901 gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa gaagcctata
      961 aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt tttcttctga
     1021 aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata aagactaatg
     1081 tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct gacagcatcc
     1141 ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg cctaatcgca
     1201 taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg gcaatcggga
     1261 tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc aaatgaacgg
     1321 gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc ggccgtttga
     1381 ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg acttgctccc
     1441 atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct gaattccctc
     1501 cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa atggcaatgg
     1561 agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca gctgctaata
     1621 caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct aacgctaccc
     1681 ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg atcactaaaa
     1741 acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac gctcttctca
     1801 tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc ggatctgtct
     1861 gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg atttcccatg
     1921 gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt catccatacc
     1981 tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac gcgctaaact
     2041 gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca tatcccacat
     2101 ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga gacgcttgaa
     2161 ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac taggaaaacc
     2221 tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt ttccttcttg
     2281 atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta acgatgcttt
     2341 caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt cacccgcatc
     2401 tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg tcaaatcaga
     2461 tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata ctttctttta
     2521 aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg atttgttcat
     2581 tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca ttcgacaatg
     2641 tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg ttgtttttct
     2701 tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct ggtgaaacgc
     2761 ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa gcagcatgct
     2821 gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt tgatccagtg
     2881 cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt ttatctggat
     2941 atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat accggatcga
     3001 gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat tcttcatgtt
     3061 cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc atcaaggtct
     3121 gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct gaactattat
     3181 acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt tcgaaatcat
     3241 gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct acgatctctt
     3301 ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct tttttgtctg
     3361 cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt accgtagctg
     3421 tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa ttttacgatt
     3481 tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat ccatcaattt
     3541 ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc tgctcatatc
     3601 tagccatttc actacaataa aaaactgaag aataccattt taggattcct cagtttttca
     3661 tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac ttcctcacaa
     3721 aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca caaggcaaaa
     3781 acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat catacgctct
     3841 ttgatttgat ggatgccgct ttcccgttat atcttctaaa ctgtttccca tttttttgat
     3901 catctgctga taatcttgct ctcggatggc ttcagcaagt ccgtggatgt cttgatgttg
     3961 gatggcttgc gtatctactt ctctaaacac tgtacgtgta gagacactca ttcttggttt
     4021 gaccaacaca acccagcact gtggcataga aggtaatact tggatttctt cccctttgcc
     4081 accgacaaat gccgtacttc cgtagacaca gtaaggaaca tctgtaccga cttccaaacc
     4141 gatttctgcc agttcttcta acgaacagcc cagattccat aattggttga tccctcttag
     4201 agcagctgca cagtctgtac ttcctccgcc taatccagct gctactggaa tattcttctt
     4261 aacaaagatc ttgacaccgg ttttgatatt gtatttattt tttagcaata aagcagcttg
     4321 ataaacattg ttccgttcat ctaccggtaa aaatgcttta tttgtttcta caatgatttt
     4381 gtcttctgtt atttcttcaa aaaatagatg atctgccaag tctacgctcg acatcaccat
     4441 ttccaactca tgatacccat cttgccgtct atataataca tccagtccta aatttatctt
     4501 ggctggcgct ttttcaatca attccatcct ctcacccatt cgaaaagtct aagtcgcaag
     4561 tattctatca tagggaaaaa taaaagtata gcttatgtct gcctagtggt tgattttgtc
     4621 atcgtttcaa cagctttcac agaattttct cttcataatc caaacaagtt ctagtgattc
     4681 ttataattct gtgagcactt cttctgccaa actcggaaca tttccttttt cttttcctat
     4741 cagactgagt agctgcatgg tagcaaatcc gaaattactt cctttcctat aagcaaaata
     4801 acgtccttcc caatgatcga catattttaa tttcttttca taagcagact ggaaggaata
     4861 gaaaaaatta ccataattat atacgatatg catgactttt tcttgaaaga aagaaaaatc
     4921 tgtttctcca acattagcta atggagctaa gcctagattg gcttccgagt agcccatttt
     4981 ctcgtataca ataaacagat tggctaacag atagtcagct aattcctcag attctttagg
     5041 taagacccgc aacaaatcat atcccgcctg cttcttcgtc atcggctgag cggtgatgaa
     5101 gccaacgact ttttgatctt tacggacaat accgacaccg ctagataata catatgattt
     5161 atcaaatcgt cccacagaga agtatttttc tcgtgcacct tgaagccatt tttgtgaaac
     5221 ttcatttaca gcttgatgta attcttccgg cagttgatcg taccaagtaa aatggtatcc
     5281 ttctttcatc aaacgttgat attcaagttg ctgactcagc cgagacaagt cttctgttgc
     5341 tagatcaaca atccctgctt cacctacttt cgtgaagtga aaacccaatt catgaagcat
     5401 cactacgtag ctttctgata tcttataaaa cgcaagctga tagcctaaac aatctgcttg
     5461 tttcataaac tgattcgttg ctgcccgcat cattttagga ttgccaactg gatctcctaa
     5521 aacaaagcat ttgtttcctt tgatctgata accaaaaacg acttgatctt ggtcgtcttc
     5581 ttggtaataa aaataactgt aacctgacaa gcgaagctga tggcttgtag ctgtgcctcc
     5641 gtatgtttcc aataaacggt caaaacggtc tttttcgtac tcactgccaa gcttcgtagt
     5701 atcttcagct aaatattgat aaagcgccac taaagctagc aatgagatgc ccaaaccaat
     5761 aagccctgag aaccacacat catctgaagg aaacaaaata aaatgatttc ctgttattcg
     5821 accgactaaa aagcgtccgc tgtgatagcc tgcaattgcg taaacgataa acaaaaagcc
     5881 gaacaagcta gcatcaaaga ttatagctcc ccaagaataa acgaattttg ttcgataaaa
     5941 ttcttttctg gataaccaca cagctaacag aatcaagaca tataccaata tcaactgcca
     6001 agataacgta cgtgtgattg tatttaaaat accaaaagat agtaaagcaa tcgtaggcag
     6061 aaatgccttt ttgactttta ctgaaagtgc tctagcaagg ccgagtaata aaaagccaac
     6121 caatagattc actgtctgat ctaaaaaatt gaatgagaac ggcaataata cttgaaatag
     6181 ccgactgatt gcagacagat tagtgattgt tgatagaagt accatcatga tccccgcaaa
     6241 atagacagca cttacgagca cgatatgcgc aaccttttgg aaaaacagtc gcggaatgtt
     6301 atctaaaaat cgattgattt tgactcctgt atgtgtgatg aacaatagaa gtcccgtcat
     6361 aaaaggtaga acataataaa acaaccgata atataatagc caaactactg ctacatcttg
     6421 ccgaacagac aattgactca ttcctaagat catcaaaacg tcaaaagtcc ccattccacc
     6481 tgggaccatc gtcagcattc ctatcaatgt agcaatcatg aacataggat agatcgaaag
     6541 catcgaaata tctattctca tcaacttgcc gatgatcaaa aataccgcaa gggctcccgt
     6601 ccattgtcca aaggatgcca gtagcagacc aagcaagcct tttggataaa actcgaagaa
     6661 caatgtcttt ctctttagat aagcaaaaaa gaaaatgact ggtgctaaaa aactaccgcc
     6721 gaaaagccag atccaatagc ttcgaaaaat actttctgac tgaataaaaa agacatcaat
     6781 aaatgtaaga aacgaccata cagacaaacc agtcagcata aatagtgcga cttttgatac
     6841 agtggctaaa accatttttc tatttgtctg ctttccataa aaactagccc gcagacttgc
     6901 tcctaccaca ccaccaaaac cggcgagatt gttgatcgtg tttgttaccc acgcagcaag
     6961 aagaaactct tttctttcca tttttggttt tccttgtttt tctaagactc tgatcgtcac
     7021 ccaatcatac aacaacatcg gagtgacacc cagcagtcca gccagaatca tgagcaggat
     7081 cgtcatgcgg ctttgttgtc ccatcctttg ccaaatatct tgccaactca tgccattagc
     7141 aatatttgcg acttgatttg ccacaaagac taacacggag ccgaaaaaaa tcagttttaa
     7201 aatcagacta tgttccttta tccattgcag catcaagtat cgtttatttt tcacattttc
     7261 ccattcctaa cttgtatact tttgtgtcat accccaatgt tatcttattt attttaattt
     7321 ttccacttta gacctgttct ttgcttgcga tgatagaaaa agaaagatat cctaatgcta
     7381 aaggagagga taacatgaaa gtaggattta tcggcacagg gaatatggca caagctatta
     7441 ttgatggttt attaaaaaaa ggaacagtct cgaaagaaga ggtgttcgtt tccagtggac
     7501 attttgaaaa tgcacaaaaa tttgctgctg aaactggcgt ccatgcatgc aaaagtaatc
     7561 aggaagttgc atcacaatca gatatcgttc ttttagccgt gaaaccaaaa atcattgaaa
     7621 aagtattgga agaactaaga gaatatagcc agaaagtctt attcatttct attgcttcag
     7681 gaaaatcatt aacagattta gaaacagcgc ttgaagatcc tcaggcctcc atcgtacgtg
     7741 tgatgccgaa tgtcaatgtc agcgtaggag caggcgtttc tgcgatttgc aaaaatgaag
     7801 ctgccacgga ggaacagctt cagcaagcta cgatgatttt tgaagcaatc ggcagcgtct
     7861 atcctttagc agaatcagat ttcagtacat tcattggcct tgctggcagc tcacccgcat
     7921 tcgtttacat gctgatcgat gcgatgggac ggactggtgt actgaatggt ttgtccaaac
     7981 agacagccac tgagattgct gcaaaagcaa ttatgggttc ttgtcaaaaa ttcctttcaa
     8041 gtgataaaaa tccatgggaa cttgtagacc aagtttcttc tcctggcggt acaactgttg
     8101 caggtgtagt tgccttagaa gaagcaggat
//
LOCUS       DACEOE010000109         8109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEOE010000109 DACEOE010000000
VERSION     DACEOE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.111"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            143..1882
                     /gene="yfcC"
                     /locus_tag="IW774_001984"
     CDS             143..1882
                     /gene="yfcC"
                     /locus_tag="IW774_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1154321.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2039..3223
                     /gene="iadA"
                     /locus_tag="IW774_001985"
     CDS             2039..3223
                     /gene="iadA"
                     /locus_tag="IW774_001985"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1154322.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3334..3537
                     /locus_tag="IW774_001986"
     CDS             3334..3537
                     /locus_tag="IW774_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1154323.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3638..4300)
                     /locus_tag="IW774_001987"
     CDS             complement(3638..4300)
                     /locus_tag="IW774_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154324.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4313..5815)
                     /locus_tag="IW774_001988"
     CDS             complement(4313..5815)
                     /locus_tag="IW774_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1154325.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNLKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6363..8042
                     /locus_tag="IW774_001989"
     CDS             6363..8042
                     /locus_tag="IW774_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1154326.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
BASE COUNT         2521 a         1438 c         1748 g         2402 t
ORIGIN      
        1 cataggagaa aaaaaacaaa atagaaaaaa gtgattttct aataaattat tcatgtggaa
       61 atgtcatttt tactgtataa ttcatttgat tatccatttt ttattcatca aaattactga
      121 aaaaagaaag gaagaaataa atttgaagaa aacaaattct tcaggaaaac cgaaacgcga
      181 cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag tcatctttgc
      241 atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata ttgaatacaa
      301 agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt caagattcct tccgttatgc
      361 ttacgagctg gataagtcgt atgtgtttga tcaattagaa gaactgcaag atcatccagc
      421 agaacgtgaa aaactggatg ttccggaaaa aggattggaa aaagtcattg ctgacggaga
      481 aaaaaatctt acgcaggaaa aactagatga aatctccttg acagatgatg tgctttacga
      541 tcagtacggc gaaaatatct atgatacttc taaaaaattg cataaaacag ccaaaatatg
      601 gggaacagat gattttggcg gctttggttt cttaaatttt gtttttgaag gactagtatc
      661 tggagataag tatggatctg ctgtagggat cgctgcattg atccttgttg ttggaggtgc
      721 ttttggtatt attatgcgta ccggagcgat cgacgcaggt atctatgcgt ttatcagtaa
      781 aactaaggga ctggagcgtt tagctttacc gctattattc tttgcttttt cattcggtgg
      841 cgctacattc gggatggcag aagaggtaat tccattctca atggtgatgg ttccatttgt
      901 cattgcgtta ggatatgact cgatcgtagc agtaaccgtg acttatgtcg catctcaagt
      961 cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg gtggctcaag ggattgctgg
     1021 aatccccgtg ttgtctggtg cgacattccg attgattatg tgggtagttg ttactgcatt
     1081 agccgctggt tatatgatga tatatgcaga aaaaatccgt aaaaaaccag aaagttcgtt
     1141 gacttacaaa tcagatgatt atttccgttc tcatatcaaa aagacatctg atgaaaataa
     1201 aaaattcatg cttgggcata aattgatctt attagagatg ttagttgtcc ttgtctggat
     1261 cgtttggggc gtgactcaaa aaggctatta cattccagaa atcgcttctc aattctttgt
     1321 gatgggatta gttgctggaa tcattgctgt tcttttcaaa ttagatggaa tgaaagtcaa
     1381 tgacatcgct tcttctttcc aatctggtgc agctgattta gctggtacag caatcgtagt
     1441 aggtatggcc aaagggattc ttcttgtctt gggaggatct gatgcatctg ttccttcagc
     1501 tttgaatacg attttgcatg ggatcgggac ggcgttgacc ggtgttccag ctgtcattgg
     1561 tgcttgggcg atgtacatct tccaaagttt gttcaacttg gttgttacat caaattcagg
     1621 acaagcagct ttgactatgc caatcatggc tccactggca gatttagtag gtgtatccag
     1681 acaagtagca gttcttgctt atcagcttgg ttcaggtttt atggacgctt ttacacccgt
     1741 atcagccagc ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa aatgggcaag
     1801 attccaaatc aagatgcaag gattcctgtt tgttttagga actattttca tcatgattgc
     1861 cattgcaata ggtctgcagt agtactggag acctgtaaat aaaaaagtaa tccaaacagt
     1921 agataaggac tccaagaacc gtcattttac ggtaaaatca aaggagtaaa gatcctatcg
     1981 tttggattat tttttaagat cctatcatca gttatactta aagaaagagg aaaaatgtat
     2041 gaaaatcatt cgacaaattg aagtgtttgc accagattac ttaggaaaaa tggatgtact
     2101 gattgccggt gggaagattt tggctgtaga agaacagcta gaaggcggct acgaaggagt
     2161 ggaagtagaa gaactttcag gtgagggaaa agtactaacc ccgggtttca ttgactgtca
     2221 tttccatatt ttaggtggag gaggagaagg cggttatcaa aatcgaacgc cagaagtcac
     2281 attgagccaa ttgactacag ctggtgtaac gacagttgtc ggttgtttgg gaacagacgg
     2341 agaaggtaga gatatgactg cgttgatcag caaagcgaaa ggtctggaag cagaaggaat
     2401 ctccacttat gtatacgaag gttcgtaccg attgccggta aaaacagtaa cagattctat
     2461 catcaaagac tttttgacaa ttgataaaat tatcggaatc ggagaaatcg cagtatccga
     2521 tcaccgttct tctcagccaa gttttgaaga gttcgctcgt gcagtagcag atgctcgtgt
     2581 aggaggcatg ttatccggaa aagctgggat catcaatgtc cacttaggcg gaggaaaaag
     2641 aaagctagag ctattgacac gaatcgtaga agaaacagaa attccgatca cacaattttt
     2701 gcctactcat gcaaatcgca caccagaatt atttgaagct tgcgtggcat ttgccaaacg
     2761 tggtggcacg attgacttca ctgctagcga agatcctgat ttttgggaga agacagatgg
     2821 tgaagttcgc ttcagcaaag cactgaaacg attgatcgag gaagatgttt ctttagataa
     2881 tttcacgatg acctcagatg gacaaggaag cttgccttat tttgatgaaa acaatcattt
     2941 cctagggtta ggagtaggaa gtgccaaggc attgctagta gggatcaaag aagctgtgca
     3001 aaaagaaagt atccctttgg aaatcgcctt acgagctata accagtaatc cagcacgtat
     3061 attgaaattg gataaaaaag gaaaaatcga aattggtgct gatgcagatc tgtgtatctt
     3121 ggataaagaa acgcttgata ttgatacagt tatcgctaaa ggagaaataa tggtccaaga
     3181 aaaagaagtc aaagtatggg ggactttcga aaaaagtttt taatcaacag ctctaactat
     3241 cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta ccctgaaggt
     3301 agatcaataa acaaactaag aggaggaagc acaatggcag atttaaaagg acgttctaaa
     3361 gatatgaaag ataaagtagt cggtgaagga aaagaagcat acggcaaagc aacagacgac
     3421 aagagcaaag aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa agaaaaagct
     3481 cgaaatttag gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa aaaataaaaa
     3541 ccacttatta ctaattgaat caagcaaata aaaaatccga accatatctg cgagacagat
     3601 atggttcgga ttttttattt gatcattatc ctgtttttta aacttcgatt ttttgaaatt
     3661 ttttattttt tagttcaaag acttcatcac agctttttgc tacttcaatc tcatgagtca
     3721 cgatgataat acagcgattt tgttcatggg cgatttcttg gaaaagtttg acgatatctt
     3781 gtgaagttgt ttcatctaga tttccagtag gctcatccgc tacgattagt tgatgttcac
     3841 agcagatagc acgaacgata gcgactcgct gctgctgacc tcccgaaagt ttggtcacct
     3901 gtttttttgc taatatttca tcaattccta cacgagccag tcgatctaaa gcaacttgtc
     3961 gataattctc ttgcttggtc ttagtgattt ccattgctgc tatgacgttg tctagagccg
     4021 ataaataagg aagcaagttg taagcttgga aaataatcga gacatcattt ttacgataac
     4081 ttcttaaccc gattttgttc agtgaacgat cttgatagta tacctttcct tcttttggtg
     4141 catctagtcc agaaataagc gaaagaaaag tcgtttttcc agaaccactt gctcctaaaa
     4201 tagcgtacat ttttccagct tcgaaattca gttctacatc tttataaagc gcatcttgcg
     4261 gatcttgata ccaaaatccc aattgttctg tccgtaacat caaattcctc ctttagttta
     4321 ataggatttt tcttgggttt aatcttaata tccctataga agctaaaaag acagaaaaca
     4381 gactgattcc aaatgctact ccggctaata gtcccaattg tgcaggtttg acattgattt
     4441 ccaattcgct gacttgatca gaaacagcaa atggattatt cgtggttcct tgttgaccat
     4501 cgcgttgacc gccttgttct tgtctgttac ctggcatttg tccaccgcca ggagcttgct
     4561 ggttttgtgt cgtggtttct gtctgttggt caagtagctg ctgtcccacg gcattcgcga
     4621 cgacgtttcc gctaagcgaa gcaactccta atgcaagaat catacaaata gccacttcgg
     4681 taaacaattg gagaatgatt ttacttctgg attctcctaa agatagcaag acgcctaact
     4741 catgccgacg ctcccgaatg gtaatcataa tgatcagagt aagtatgata ataccggcta
     4801 cagccactag taaaacaaca tttttagaaa aacttgccac gttgtttaaa ggttccaaca
     4861 ttgactgata catggaatca tttgattgta agctaaatgt atctgtgtcg atcagcttct
     4921 cagcttcttt gagaaactca tccattttat ctggatcaga tagagtgtaa atagcagaat
     4981 ctatcgtgtt atcgtcagat gtcccattta gttcatttgc aaaagtgtaa gaagtgtaaa
     5041 gtgtattcga cggattcatg aagttgaatt tcatacccat actgccgctt gtttcgctgg
     5101 attcataaat tcctttgatc gtcatttcat aagtggtatc ttcatcttca gtactcgtga
     5161 ttgtgaaagt atctccgact gccaggtcat ttgtttctgc tagagtagaa tcaatgacta
     5221 cattgtttgt gcctttgtcg ctgtcatcta ttccttcacc atccgtgatt tttgctgttc
     5281 catcagaaaa attactattt tgtgcagttg ctgacacacc actgatttga aagtctgctt
     5341 gagtcatttg cggattcccg cccattcctc cagcaggttc accgcctgca tcagtagttt
     5401 ctgaactgtc ttcacttgaa tcagaactag tgatggcagt tattccatct tttgccatag
     5461 ctgaggcgga actttcaaag gaataactct ttaaattatc taattcagct attttttccg
     5521 cgtccgttaa ggaaactggt gtaagagaaa aactgcctgg atctggacgt tcttcagtgg
     5581 tagaaccgct tgaagaagtt tgttcctgtt ttttaaatga agcttctcga ttggcactta
     5641 aagtcactgt tgctccaact tctttttgtg cttgattcgc tgcttcttct gcagcgcttc
     5701 gaatcgtgag gccagctagc acaaaaataa gaatggctgt gaacacggca aacaataaca
     5761 atgaacgtcc ccattttact tttgtacttt tccatgcacg tttcataaaa ttcatatttt
     5821 gcatccctcc tagtagatgc atttgataat agacaaccta tcttaaagct agcttaaatc
     5881 ttatgctgtt tgcacaccat caacatgagg ttctgtatat ataaaaagtc actatagtta
     5941 ttgcaaaaaa tgtggtaaaa tgaaaaaagt atgtttcaaa ccgacgaaga agaaatgaaa
     6001 gaggaatagc aaaagaaatc ctagatgcta cagagggatt tttacgtgca tagttgttct
     6061 aacaattgct gatactagcg atttagttta tttgttgtat aataatgggg aacagctgaa
     6121 tacttatata agtattctgg ctatagcgtt tcgcaaaaga tccattttgt taatgaagta
     6181 tatccaaaat aaaatgccca tccaactgtc agaacgcgga tgtgctggta attttcctgc
     6241 tgattctcca taagttgaga aatagtgatt ttgcatatgc ttcattggaa tggctcatat
     6301 atttagataa agaagaaaat cgacatcttt aaacttggat gtcataagaa gaggtgaagt
     6361 ttgtgagtac cattaaaatt atcccattag gcggcgttcg tgaaaatgga aaaaacatgt
     6421 atgtcgcaga agtgggcgag gatattttta ttttagattg tgggctaaaa tatcctgaaa
     6481 atgagttgtt ggggatcgac gtcgtaatcc ctgattttac gtatttagaa gaaaacattg
     6541 atcgtgtagc tggtgtcttt ttaacacatg gacatgcaga tgcgatcggg gcacttcctt
     6601 acttgttgtc aaaaatttct gtaccagtat tcgggacaga gttgacgatc gagttagcaa
     6661 aattgaatgt cagtcgtaat gaagcagcca aaggatttaa ggatttccat gtggtagatg
     6721 agcatacaga aatagacttt ggtgaaacag tagtgagctt tttccgtacc actcatacga
     6781 taccagattc agtggggatc aatctgaaaa caaaagaagg tagtatcgtt tatacgggag
     6841 attttaaatt tgaccaaacg gctatcccaa tgtatcaaac agattatgca cgattggcgg
     6901 aaatcggcaa agaaggagtc ttagccttac taagtgaatc ggcaaatgca gaaaatcctc
     6961 agcctgtagc ttctgaagcc caaatagcag atgaagtatt tgatacgatc aaatattggg
     7021 aagggcgtat catcgtcgct tgtgtagcaa gtaatttgca acgagtgcaa caagtattgg
     7081 atgcagcaga aaaagcacaa cgaaaagttg tcttgactgg tcaagatttc gaacgaatta
     7141 ttcgcacagc gatgcgttta gaaaaacttc aattaccaag tgaagatctg cttgttacgt
     7201 tgaaagacat gaaaaactat gatccagaag aactgatcat tttagaaact ggccgaatgg
     7261 gtgaacccat caagtctctg caaaaaatgg caaacggtac acaccgtaat gtcaagattg
     7321 aagaaggaga tttggtttat atcactacta cgccaagcat cgcgatggaa accatcgtgg
     7381 cgaaaacgga agatatcatt tatcgagctg gtggaacagt gaagttgatc tctgagaata
     7441 tgcgcgtttc tggccatgcc aatcctaatg acttgcaatt aatgatcaat ttgatgaaac
     7501 caacatattt tgtgccagtt caaggagagt atcgtgaatt agctgctcat gcagatttgg
     7561 ctcatgcagt agggatgcca tacaaaaata tctatattac tggacgcgga gacgtgctgg
     7621 aatataagaa caagcgtatg agtgttgcag gaactacaag tgcagaaaat gtaatgattg
     7681 atggattagg cataggtgat atcggaaaca ttgttttacg tgatcgaaaa atcttgtcag
     7741 aagacgggat tttcgtagcg gttgtcacta tcagtcggcg agaaaagaaa atcatttcaa
     7801 aacctcaaat tacttcacgg ggattcgttt acgtaaaagc tagccgtgat ctgatgaaag
     7861 aaagcagcaa tatgattgaa acaatcgttg agaaacattt agaaagcaat gattttgagt
     7921 ggagcaaatt aaagcaagac atccgagatc aattaggccg ttacttattt gaacaaacaa
     7981 aacgccgccc agtgatcttg ccagtcatta tggaagctac tcaacgtcga aataaaaaat
     8041 aaacaaaaag agtaagatga acctctctat agagtgctcg tcttgctctt tttttgcgaa
     8101 aaacaaata
//
LOCUS       DACEOE010000110         8050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEOE010000110 DACEOE010000000
VERSION     DACEOE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.112"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IW774_001990"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IW774_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1154327.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IW774_001991"
     CDS             complement(322..891)
                     /locus_tag="IW774_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1154328.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IW774_001992"
     CDS             1077..1979
                     /locus_tag="IW774_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1154329.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IW774_001993"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IW774_001993"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1154330.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IW774_001994"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IW774_001994"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1154331.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..>8050)
                     /locus_tag="IW774_001995"
     CDS             complement(5002..>8050)
                     /locus_tag="IW774_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1154332.1"
                     /translation="QLMKLSSRKMIQEKLELAEMTETDHLAFVVPANHSVSEAFNKED
                     NFFGHWQFLLENVFTRNFYIGLTENTPDQLPEWYQYVIKENYSLCALHPIASLQQEEL
                     FALEVMKHIKEGSQIPLENLSASLPLTANVYLLSEQEMIKRFEKAGLSQAVIGAQRLA
                     ESISFEFQFHQKLLPHYPVPNGKQAGEYLRELCYSQVERRITDYTKEYQERLDYELSV
                     IHQMGFDDYFLIVWDVMAFAHRNKIVTGAGRGSAAGSLVAYVLEITDVDPLEYDLLFE
                     RFLNPERFTMPDIDLDIPDNRREEVLNYVREKYGQYHMAQIATFGTMAAKMVLRDAAR
                     VFGLSQSEANRWSKAIPNQLKITLTQAYKTSHQLVELVQASEKNQLLFKTAVILEGLP
                     RHVSTHAAGVVISDKNLLELVPLQRGSDEAFLTQYTMNEVEAIGLLKMDFLGLRNLSI
                     IDFTLKAVKRVEGYEIRLKEIPLNDAKTLLLFQRGETSGVFQFESAGIRNVLRRLDPE
                     NIEDIAAVNALYRPGPMQNIDTFIARKKGKEAIRYPDDSLIPILKNTYGVIVYQEQIM
                     QIASTMAGFTLGQSDILRRAVSKKKKDVLDEEKSHFVSGALQQGYSEKTAATVYEYIE
                     KFANYGFNRSHAFAYSFVGFQMAYLKAHHPGAFFTSLMNSVRHNTSKLKEYIAEARKN
                     KLKLSSPSINQSNSSFELVNKEEIRFGLTAIKGIRRDFVEDILNERKQNGPFQSVDQF
                     LIRIDKRWLKLELLQSLTAVGVFDELSPNRKQLMLDLEGKIQNIVYSGGSLDLLGIMA
                     LKEEEVADYTLEERLKLEEEYLGIYLSGHPAEGFDRLRLAKQVQLITDIVPNQPVNLL
                     VMIKEVREIRTKKGELMAFVEGTDSSGEISVTVFPTLYRKYRNLFAVDRVIFVQGRSE
                     ISKYNQELQLIGESFADPNQLEAQYPSQTCYLRITEEREDPEIMKQLQVIFQKHPGYI
                     PVVMYHEKTQRKVVLSDAYWVDDSKSIKGQLIYLLGEDNVVFK"
BASE COUNT         2276 a         1726 c         1414 g         2634 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc
//
LOCUS       DACEOE010000111         7993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEOE010000111 DACEOE010000000
VERSION     DACEOE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.113"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            214..999
                     /locus_tag="IW774_001996"
     CDS             214..999
                     /locus_tag="IW774_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1154333.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1012..1863
                     /locus_tag="IW774_001997"
     CDS             1012..1863
                     /locus_tag="IW774_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1154334.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(1987..2439)
                     /locus_tag="IW774_001998"
     CDS             complement(1987..2439)
                     /locus_tag="IW774_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1154335.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(2904..3272)
                     /locus_tag="IW774_001999"
     CDS             complement(2904..3272)
                     /locus_tag="IW774_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1154336.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3422..3904
                     /locus_tag="IW774_002000"
     CDS             3422..3904
                     /locus_tag="IW774_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1154337.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            3941..4951
                     /locus_tag="IW774_002001"
     CDS             3941..4951
                     /locus_tag="IW774_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1154338.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5066..5779)
                     /locus_tag="IW774_002002"
     CDS             complement(5066..5779)
                     /locus_tag="IW774_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154339.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(5969..6559)
                     /locus_tag="IW774_002003"
     CDS             complement(5969..6559)
                     /locus_tag="IW774_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1154340.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7082..7753
                     /locus_tag="IW774_002004"
     CDS             7082..7753
                     /locus_tag="IW774_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1154341.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
BASE COUNT         2691 a         1290 c         1536 g         2476 t
ORIGIN      
        1 aatttttgta aattacttct tatttcttga agctggccac ttctgtcaca acctctttct
       61 ttatagaaat aaaaggacac tagcaattca tgaaaacgat tgctagagcg tatcatttgc
      121 tttagactat atatcttggt gtacactcac tttaagacgg aaataaaagt tatccgtaat
      181 aacaggttta ggtatgaaaa aggagtgggt actatgtata aggaattaga aaacaaagtc
      241 gcagtagtaa ctggtggctc aaaggggatt ggaacagcga ttgctcaacg attcgggaaa
      301 gaaaaaatga atgtagttgt caactataac tcggataaag aaggagcaga aaaagcagtc
      361 aaagcaatcg aagaggcagg cggaaaagca gtctctgtac aagcagatgt cagttctgaa
      421 gaaggaatac agcgtttact tgatacggct ttagaaaatt ttgggacact ggacgtttgg
      481 attaataatg ctgggatgga aaatcagcat ccaacccatg aacttagcct tgaagattgg
      541 gaaagagtat taaaagtcaa tttgactggc gtttttatcg gaacgaaagc ggctttaaat
      601 tactttacag agaatgataa aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa
      661 atcccatggc caacgtttgc tcattacgct gcttcaaaag gcggagtaaa actattcacc
      721 gaaacggtag caatggagta cgcaccaaag ggaatcagaa tcaatagtat aggacctggt
      781 gctatccgta cacctattaa tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg
      841 gaaagtatga tcccactagg acgcgtaggt gaaccaaaag aaatagcggc gacagctgca
      901 tggttggctt cagacgaatc cagttatgtt acaggtatta cactatttgc agatggtggg
      961 atgactttat atccttcttt ccaaggtgga aaaggataag ggtgaataaa aatgggttta
     1021 ttgattgcat tgatcccggc agttgggtgg gggatccagc cactgatttt gaaaaagatt
     1081 ggtggcagac caacaaatga gatattagga actggtattg gagccgtgct cgttggctta
     1141 cttgttcaat tattcgcttc tccagctggt atttcagtga ctacattttt gattagttta
     1201 ctttctggtg cattctgggt catcggacaa acaggacagt atacatcttt caacttgatt
     1261 ggtgtttcta aaacgatgcc aatttcgact gcattacagt tagtcggaac ctcattgatt
     1321 ggtgtcttcg cttttggaga atggtctgga agtatgggaa aaatcattgg tgcagcagca
     1381 atcattcttc ttgtatttgg ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa
     1441 ggcggtatca caaaaggaat cagtctatta gcatcaacaa gtattggtta ttgggtttac
     1501 agtgcattgc ccaaactagt tgatgcaagc ggactttcca ttttctttcc gcaaatgatc
     1561 ggtgtctttt taggggcagt gatttatgtg attttccgaa aaccaaccgc attttctgaa
     1621 gacaagagtt ggaaagcaac aatagttgga gccatcttca gtgtgtcagc attggcttat
     1681 attttttcag caaatgaaaa tggggttgct actgcatata tcattacaca gctaaacgta
     1741 gttatctcta cattaggcgg tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa
     1801 aaattaacga ttatcggatt attgctgatc gtagtaggaa gcatggtcac ggtctttatt
     1861 tgaggataaa gatggtaaaa tctcatttat taaaaaaata tttgtttgaa ccaaacagta
     1921 agtacttata aaaaaatagc gctagaattt ccaacatctc tggaatttct agcgcttttt
     1981 aattgtttaa gatacaagtg aaatatctgt ctgcatcaat aatttttcat tgatccattg
     2041 actaaagttt acgacataat tgaagttacc accgattagt atctccgcat gattgtcttt
     2101 ataaagatcg tattgtaaaa gacggtcttt tttatttgcg taaacatcgt tatggtactg
     2161 gactaattgt tctcggaatt gattgattgc tgtaaaatca cgatactgaa ggttgaaacg
     2221 catcgtgatt ggtgcattta gcatgaatcg ctgcatttct tttgctgctt cctctgcttg
     2281 atctccgtat aggaactcaa aaagattacg ttcagaaaat ttccattgcc cgccaacctt
     2341 tgttcctttt agttttcctt catgaagata atttctgata gaacgtgtag taacacctaa
     2401 ttgttcggct aattcattta ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat
     2461 ggttctactg acttagttga tatttgctta atcacgaaat gtagaaagaa cgattttaat
     2521 taaggtcatt gtaaaaatca agtcagaaaa ttctaacttt tctttctcta aagaaattcg
     2581 ttctctccta aagtgatatt atatttaaat atacctgaaa ataatgggca gtattatgaa
     2641 agatcactga tattttctcg tattttttcc ttattcaaaa gttttgcagg aaaattgctg
     2701 aaaaaataat tttactaagt ggaaaattca aagccattca atcagtgatt tttttcagca
     2761 caagaaagac tccttataaa aatagcagat actttgtcaa ttaagtgttt aaccagggat
     2821 gataaaaagg ataggattga acaaaacctg tataagctga tgaatagttt cctcgccagt
     2881 aaaaggttta ttcgtataaa gctttatcgc caaaatttat catgaccttt tttatcttca
     2941 atattaaatg aaatcaattg ttcaatcaat tcccagttga ttacactttt ccaaggaact
     3001 ttgatgacgt tatctgtata tacgtagcct gcttcttgga tatttttttc aagggcacga
     3061 atcgctgctt tttccggtgc gatagaaaaa tgatttttag caaaactaaa tgcaataata
     3121 aaggtaccat ggtcagtaaa cataggttgg ttccatttta cagttgtgtc tagctgggga
     3181 tacgtttctg ttatcttagc aaaaaggtct ttgatctttt gttgatgttc tttttctttg
     3241 atctgattga tgatcggctg aaatttttcc attctaaaac gcctccatta agtggtttga
     3301 tttattttac cagaaaaaca atagttatgt ataaaaaacg aacaaaacac aagtaatgtt
     3361 gtaaccgcta tatttatatg ataagatgtc tcaaataata aaatagaaaa gagacataaa
     3421 aatgattgac ttggcaaaga aattcttaca cgaaaatata tattttgagt tgtatcatca
     3481 tcgagccatt tatacaaatg aagatgcttt ggtaataaag aaagaacaag gatttacagg
     3541 tacagagacc aaaagcttat tcttaaaaga taaaaaagat aataactacg tatatctgac
     3601 ttttactacg aaacaaacgg atttcaaagc attggggaag atcgtcggta aaaggctttc
     3661 tattgtttca tcagaacaca tggaagaaca aacgggacaa aagcagggcg ctgtttctcc
     3721 gtttggttat gaaaaacaga ttccgattat tattgatgaa gagctcttat taaatgaaaa
     3781 acttgttttt gcaccaggta gaccagatca aacgatggtc atccaaacgg ttgacttaaa
     3841 caaaatcatc cagctacttg agatgccgat atttttgatg ccggcaaacg aaaaaaacaa
     3901 ttaaccaagt aaaatagtgc atacttggag gtgaagcaac atgataagtt atccattatt
     3961 agaaagaaat acaacgattg gggttactgc accatcctca ggcgtatcag aggaacacca
     4021 ttcgttactc gaatcagctt taaagagaat ggaagaaaaa tctttccaag tagttactgg
     4081 aaagacgctt tggacgcaag ataaggaaaa atcaggtcca gcaagaaaac gagcagatga
     4141 attcaatcaa atgatgcaag atgaaaatat ccaaatgatc tttccgcctt ggggaggaga
     4201 attattgatt gagattctcg attttgtcga ttttgatgct ctacaaccaa aatggatagt
     4261 aggttattca gatattagta cgttgctact tgctgtcaca ttaaaaacag ggatagccac
     4321 tgcacatgga atcaacctta ttgatttaag aggagagtac ttggatgaaa cgacagcaat
     4381 gtgggaaagt gtactgaaaa caagagaagg agaatcgatt ctacagcaac cttctgaaaa
     4441 gtatcaaaaa gagtggcccc atgagcattc atcatcttct gttttccatc tcactgaacc
     4501 aacaaattgg caatcagtca gcggagatgc agtaaggatg aagggacgat tattaggtgg
     4561 ctgtattgat acgatcagtc atttaatcgg taccccttat ggagatgtcc aatcttttaa
     4621 aagaaagtat aatgatggaa agccgatctt gtggtttcta gaaaattgtg agctatcgtc
     4681 agttgattta agaagatcac ttgttcagat gaaactggca ggatggtttg atggcagctc
     4741 ggggatcatg ttcggtagaa gtgcagcaga ggatttgtta gattataaaa tgaaggatgt
     4801 atacgaagat ctgtcaaagg aattaaaact accgatcttg tatgatattg attgtggtca
     4861 tgtaccgcca caactgacat tgatcaatgg tgcctatgca gaagtagcgg tagaagatag
     4921 aaaagcgact gttttgcaaa cgtttattta aaaaattttt tcctgccttg gagtttatca
     4981 aagaccaaca aatctaaata gaaaagaaaa tgaaagaagc tgacagtatt ttgccagctt
     5041 ctttttgtat gatctattag aaattttaat tatccataac tatcacattg atcagctgtt
     5101 tcacatcatt tggattacct gtaggaatag ctactacaga tgcattatca ataatttctt
     5161 gagttgtttc tctaatgaac gctggataag atgtttgatc tgcaggcatt tgccaatgtg
     5221 taggaactgg ataactagtg attgtcccgt catggttact tgaatacact aaaactccct
     5281 ggtaactgta tttcccagtg agcataacca catctttcgg atagactgca ctccctgcat
     5341 cataaggggc gataggtgtt cctgcaggat aatgaataac atgtagttcg aaatcactcg
     5401 atgtatctct aaaagtatta cctctaatag ctagccaaac acgtgcgtac tcaatttgta
     5461 gatcagaata tcctgctaac ggatctattg actgagaaga ctgtgaggaa gttgtgtcta
     5521 ttgttgaaga agaaatattt gtctcaactg tactactaga tgtagaagaa gtattgggct
     5581 ctgagacttt actgcttgtt gaagatgtat tcgtttcagt agaagctgat ttctgagtac
     5641 ttgatttttc agctgaactg gtttgtctga ttgaactttt gtctgtggtc gcagatggat
     5701 cactattttc tttactagga gacgtacatc ctacaaggaa taaagagacc aacaataaaa
     5761 taaaacctaa ctttttcata taaaatacct cattattttt ttaataccag tatagcgtaa
     5821 tcctgaattt ttgaatccgt aaaaccttgt ctatgaatat aaatagagta tatcttcttt
     5881 gataaatgtc agaaaaagtg tgacaaataa ccatcagtaa aattacttgt catactattg
     5941 agaaggagta tttaattgaa gagaatgatt aggagaaagg atctccgtac tttttatagt
     6001 aatcatagaa tccaagtttg tatagacgaa tcgttttttc tcttatttct tctgtttcaa
     6061 aatggactgc ttgaagaatc aagtctgtga attctatagg agctgttcca ttcgcagtaa
     6121 tcaaattacg atctttcaca gcttgttgtt ctataaaatc ttctttattc tgataatttt
     6181 ccgattctaa ccaaggaaat tgcgcatttc cggtgtgttt gtaaccttgc aataatccgc
     6241 tctgagctaa ataatcaact gccccacaaa tagcagctac tggtacgtct gtttttaaca
     6301 atttatcgat tatttgatat aaatgatgat tttttatgtc ccatgaattg ccaccgatca
     6361 taataaaaag gtcaactttt tcaggtattt gttcgattaa gtaatcgatt tttgttttta
     6421 atcctccaat ggaagtgacc tctgattgga tagaagcagt tttgttttgc catccctctt
     6481 tttggtttag tgtgctagta atataagcac cttcccaatc ggcatattcc tctaatagaa
     6541 acgtaatcgc agttttcatt ttcaagctct cctttactat ctatgacaag gacaacagtt
     6601 ttaacataca gacattcatt aaatttggat agcgacagcg aaaacaacaa ttcttaagtt
     6661 ttagacagag tatacagaca gtcaacttaa ttaaatacga actaaataaa aatgacaggt
     6721 aatcattttt gtttaccagc attataacat tttgcataca tggatgacaa aagaaccaat
     6781 tcccacttgc ttgagtacaa tcttaaaaga aactggaagg caaaaagtaa ttatagaaac
     6841 gaaaaataaa tcatatcggg cactcagaca tgaaaacaac cctagaaata tataactagt
     6901 ccaatgaaaa aacagcgaat gccgtagaaa acaaggattc tgcgtacatt catatctcat
     6961 tttgcgagca tatctgctat tactgtgact aaatgttaat atggttaaaa tgaccgttat
     7021 attttttata taaatatcgt ataattagta tgaatataga agaaggaaag aaggtgcaat
     7081 cttggaacct aaagacaagt atttagatta cgctagggtc aataaagata aatttattga
     7141 aacaatcatc aagggaaaaa tccctgatga tgaaaaagat gcggtattca tggctggaag
     7201 cccaggagca ggtaaaacag aggttgcact aggtttagct gaaaattatg acaaccatgt
     7261 tgtgattgat gcggactatt ttagagcaca atttccagaa tacaatggaa agaactcaag
     7321 tgtttttcag aaagcttctt cttggttagt tgaacaatca ttaaaatatg tactggaaca
     7381 tggctattct ttcatactag aggatacttt tgctatcctt agtgcagaga aaaatattat
     7441 tcgagccctt aaaaataact ttcgcgttac gattttctat gtttaccaag atcccaaagt
     7501 tgcctgggat ttcactcgaa aacgtgaact agctgaagga cgtcatgtgc caaaagaaat
     7561 gtttatcaat gcgtttttta agtccagaga gaatattgaa aaagtcaaaa atagacatcc
     7621 tgaagtagtc cttcacataa tgattaagga ttatcaaaac gacatttctg aagtgcatta
     7681 tgcagcagat aatattcaat tagtactacc caatacaata tacttcaaaa gagttggagg
     7741 aggaattaca tgactgagaa agaacaagta caacaaatcg tgaagaagta taataaaagt
     7801 atcgctgatt tatcagaaaa cgcctcagcg aaagaattta aaacagtaat gaagtacgta
     7861 gcagatgaag caaatcgtaa gcagcgaaaa ctagttgggt tggataaata aattttttaa
     7921 accatatcct tgtaaagttg agttttttgt tttaatgaat taagactatt aaatagataa
     7981 ttattgtaac tta
//
LOCUS       DACEOE010000112         7944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEOE010000112 DACEOE010000000
VERSION     DACEOE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.114"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(25..573)
                     /gene="aac(6')"
                     /locus_tag="IW774_002005"
     CDS             complement(25..573)
                     /gene="aac(6')"
                     /locus_tag="IW774_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1154342.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1126..2229
                     /locus_tag="IW774_002006"
     CDS             1126..2229
                     /locus_tag="IW774_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1154343.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2422..3489)
                     /gene="rlmN"
                     /locus_tag="IW774_002007"
     CDS             complement(2422..3489)
                     /gene="rlmN"
                     /locus_tag="IW774_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1154344.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3719..6166)
                     /locus_tag="IW774_002008"
     CDS             complement(3719..6166)
                     /locus_tag="IW774_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1154345.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6355..7791)
                     /locus_tag="IW774_002009"
     CDS             complement(6355..7791)
                     /locus_tag="IW774_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1154346.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2203 a         1726 c         1359 g         2656 t
ORIGIN      
        1 tacttctgtc acaacctctt tttattattg agaatttggt cgaggaataa tcgtttttgc
       61 catccaaata tccggtttgt cccagccatt tgcatttggt aagacaccta cgattttata
      121 acctaatttt tcatagaatt catacggatg ttcacgaagg ttctggatgg aagccacttt
      181 atcaaatgta tgctcataca ggtccgtttg acttaacgtt gttccatggt ctaaatcatc
      241 cgtacctaaa taaatcgtga ttcctcctct ggaagctact tctttttcta agtaattgac
      301 taatcgagta cctatttggt tctttcgtcg ggagctttct acaactaatg gatgcaattc
      361 ccaacctgtg ataccgtatt gagggattgc accaataaat cctactaact catcttggtc
      421 taccgctgct accgcgattc gttctggatt catcatttct tctacttctt ctgccgagct
      481 gtctccatat tcttccggcc aagtcagtct cagtaaatca gaaagctgat ctttcaatac
      541 tggattatta cggtcaaatt cactgattat catacgtctt cccccttgtt cttgcttatt
      601 ctaacagtcc ttttgaaaaa acgaaatgtt ctcgctcaaa aatctttagc taccaatcag
      661 tatctctatt ctttcattat ttatcttact tataaaataa aaaaattata cagaaacact
      721 ttattcaaaa taggaaagat caggaaagcc atcaattgga ggaaaaaact ctgtttgttc
      781 atcatgatat agattatctt ttgctttaaa aaaaaccaat tatgaattga caaataaaac
      841 ctgttttcat ataataatct taatttttag aatttttaga taattacaaa aaagcaaagg
      901 gaatcggtag aaatactaag aggaacaaaa attcattatt gtcgttttca atgtttttgt
      961 acttgttcgt ttttaggata cttattgatg atttcaaggt gttcatgcca ccttattcat
     1021 cacttatcct gtcttaggtt cacctctact ccatctacag ccgcagtcag atcgatcact
     1081 ctttaaaatt ttaacccgtt tcaacgaaga aagaaggaaa tgaatatgag tcaaagtgtt
     1141 ttcttaccaa attataccat tggaacagat gcgtatgaaa aagcaaatgg aatcgtcaaa
     1201 gaatatggga caaaagtagt gctgattgga gggaagactg cactttcaaa ggcagaaaac
     1261 aaaataaaag tagctttgac acaggaaata gaggttgtcg ccagtctctg gtacgggggt
     1321 aattcaactt atggaaatat tgaacagttg atccagcatg aaagtgtcca aaaagcagac
     1381 gtcctgtttg cagtcggtgg tggccgtgct tgcgatactg taaaagtcat tggtgaaaaa
     1441 ctagctaaac cagtcttcac ctttcccact attggttcga actgttcgcc cgtcactgcg
     1501 gtatgtgtca tctataacga agaaggcgta atggacggac tcttctttcc ttcaaaacca
     1561 cctgtacaca ccttcattga tacagcaatt attgccgaag cacctgataa atacttatgg
     1621 gcaggtattg gcgatgcttt atccaaagaa gtcgaatcta ctttttctgc tcgtggcgat
     1681 gatctaggct acacggatag attaggaatc caaatcggaa aaatgtgcat tgatcgtctc
     1741 ttgacttatg gtacgcaagc attgaaagat agtgccgacg atcgtccctc atatgcaatt
     1801 gaacaagtcg tttcggatat cattgttact accggattgg tttctgtttt agttaacaat
     1861 aactataatt ctgctatggc acactcattc tattacggta cgactgttct agaaaaaatg
     1921 gagggatacc ttcacggagc atctgtcagt tatggtgtac tgaccttatt gatgatggat
     1981 cagcagttcg atttgtttga taaaatctat gtgtttatga aaaagaacca acttccagta
     2041 tcgttaaccg acatgaatct caatgtcacg accgatttag aagcaatctt agataaagcc
     2101 atggcaacaa atgatattaa acatgtgcct tatgaaatca cccgagaaaa atttaaacag
     2161 gccattcttg acgtagaaac ttatcatgct acacaagaaa gccagcaagg atataagaaa
     2221 atcagttaaa taattgctaa aacccccatc aagcaggctg tcatcaaatt gaggacattt
     2281 ctttgcttga tgagggtttt tcttgtcaaa aaaagcttta tagctttcgc tatagaatct
     2341 tttctcttat ttaagttgaa cagttcctta attaagaaaa ctgctccttt tatggtaatt
     2401 gaaaaaacga gtaggatacg attatttggc aactgttttt ttcatttgct tgcttcgcaa
     2461 ttgtccgcaa gctgcatcaa tatctgtacc atgttcttta cgtatcacac agtttacgcc
     2521 gtttttcttc aatacatcat aaaatctcaa tacatcagct tttggactgc gagaatattg
     2581 atcatgttca ctgactggat tatatggaat caagttgaca tatgctaatt ttttcttatc
     2641 ttttaataga tctgctaatt gttgcgcatg ctctggtcta tcatttactt gattcaacat
     2701 gatatattca aacgtgattc gacggtttgt tttctctaaa tactcatcca cagcttccat
     2761 caatttctca attgggaaac ttcggttgat ccgcatgatc gatgttcgca cctcattatt
     2821 cggcgcgtgc aaggaaatag ccagattgac ttgcaagccg ttattagcga attccttgat
     2881 tttgtgtgct aatccactag tggaaactgt aatgtgccga gcaccaattg ccaatccttt
     2941 ggcatcattg atgatatgca aaaagtccat tacattgtcg taattatcaa atggctcacc
     3001 gatccccatt acaacgacat gtgaaacgcg ttcgccaaga ttgcgctcat caaaataatg
     3061 ttggaccatc atgatctgtg caacaatttc tccagcagtc agatcacggt tctttttgag
     3121 cagcccgctt gcacaaaacg tacagccgat attacatcct acttgtgtcg tcacacaaac
     3181 tgacaagcca tattcttgtc tcatcagtac agtctcaatc atgtttttat cagggagttc
     3241 aaacaaatat tttactgtac catccgaagc ttcttggacg accatctgtt ttagaggatt
     3301 gatgataaaa tgctccgata atttttcgat caattgtttc gatagattcg tcatttctgt
     3361 gaaattactt acacgttttt gatacaacca ttcccaaact tgggcagctc ggaatttttt
     3421 ctcgccgttt tctataaacc aattgattaa ttcttcattc gtcaagccat aaatggactg
     3481 tttttccaaa gtattttccc tctttttctc aaagtttcgc tacatactat acatgattca
     3541 tttccttaat gcaatacttc acaaataaat cgaatagaac tttaatctca caaaaactaa
     3601 aaagacaaga catcataccg tattagaaaa gatggaaaaa aaccattttt tcttttggta
     3661 tcatcatgcc ttgtcttttt attggttatt cgtttttcaa cccttcatca cacaagtttt
     3721 acatgtcatt atcgtccggc aacgtttctg atgtttcttc ctgctcaatg aatactttcc
     3781 gaggcttgct tccttctgac gggccaacaa ctccatgagc ttccagttca tctactaaac
     3841 gtgctgctcg gttgtaaccg atccgaaatc ttctttggag aagggaaata cttgccgttt
     3901 gcatttcaac gacaagtgct tttgcttctt caaacagctc atcttgtggc tgttctggag
     3961 cacctgtcgt ttcaacttca tctgtcggca tcattttctc ttcataatga gcttcttgct
     4021 gatctgtaac aaattgaacg acccgttcta cttcgtggtc agaaatgaat gctccttgca
     4081 cacggatcgg tttattctca cccattggca agaatagcat atctcctcgc cctaagagct
     4141 tttctgcccc attagaatca atgatcgtcc gagaatctgt accgcttgat accgcaaatg
     4201 ccattctgga aggtacattt gctttgatga ttcctgtgat aacatccaca cttggacgtt
     4261 gagtagctag gatcatatga atccccgctg cccgagccat ttgcgctaaa cgaataatcg
     4321 catcttcaac ttcattgctg gctaccatca tcaaatcagc taattcatct acgatcacta
     4381 cgatgaatgg taaggttgga tgtttcgttc cgttttccaa gtttttctgt tgaacaaatt
     4441 cattgtaacc agaaatattt cttactccgg ttgccgcgaa tttttcatat cgttcttcca
     4501 tttcttgtac tactttttgt aaggcttgcg ctgcttttct agggttcgtc acgaccggcg
     4561 tcaacagatg aggtatgccg ttgtacatat tcaattcaac cattttcgga tcgatcatca
     4621 tcagttttac ttcatggggc ttagcacgca ttaatatact tgtgataatc ccattaattg
     4681 ctactgactt cccactacca gtcgaaccag ccacgagtaa gtgaggcatc ttcgaaagat
     4741 cagccgtttg tacccgtcca gaaacatcac gtcctaaagg tacttctagt aatttatctg
     4801 ggtggtttgg ttgtgcttcg atgatttctc taaaggaaac catactgatc tttccatttg
     4861 gtacttcaat accaatcagt gatttacctg gaatcggtgc ttccatccgg acatctttcg
     4921 ccgctaaagc aagcgcgata tcatctgtta agttcacaat tttacttact tttaccccta
     4981 cagccggctg gacttcgaat tttgtgacgg atggtcctaa gctggctttg acgaccttgg
     5041 catcgacacc gaaactttgg aaagtttgtt ctaatacgcc gatatttttt tcaatttttt
     5101 tatactcatc actttgatcc acagttggta tagaatcaag caaatcaact gtcggtaatt
     5161 catagtcttg attttcctgt tctgcttcaa tctcaaattc taatgaggta ccgtcatcct
     5221 ctggcaactc atctgaaaaa ccttctgctg tattggtctg ttttggtgta tctggtactg
     5281 gtttctctag attcgcagga tggatatttt cttgaaaact gtcgattgga acaaaactta
     5341 gctgctccgg ctctgtattt tctggttcct gccattcatc agcagtattt tccctttgct
     5401 ggctcaccgc ttttttcttc tcatattctg cagcttcttt ttctgcagct tcttttgcag
     5461 ctaagcgcct tgcttctttc gcttctgcgc gctgtttcat tctctcttcc tttgccgctt
     5521 gctttcgagc ttgcttggca ggatcacctt ccaataattc ctgtactcgt tctccaattg
     5581 attggatacc atttaacagc tgatagcctt ccagattact gaacaggaat gctcctcctg
     5641 ccagtaatag cactgctgct aaataacttc caggctgtgc aatcaagaag taagtaaaat
     5701 gataaaggat tgctccaatc atcccaccac caacgttatt gtctacttga ctttgtttta
     5761 tatcactggc taacaaatcc catgtcgtcc ccatgatatt tggttcccca gtatggagtt
     5821 tgccaaataa atgggcatgt aacaataata agataccgaa ataaaagagt atgccgccaa
     5881 accagcgtcg gtttttacca attgaaaact ctgtattctt aatgacaatc caaaatccta
     5941 atacggctaa taaaagacag aggatttggt aagtatttcc tgcaatgatc cgaaagccgt
     6001 ttgcaatcaa tattcctaaa aaacctaatt ttaaaaagcc gaagacagca aataaaatca
     6061 aggcaaaccc aacaccaatc gtcagcattt gttcctgctg ctgtttttgc tttttcgttt
     6121 ttttcgtctt tttccttttt ttagtaggcc gtttcttttg tgccatttat tatccctcat
     6181 ttcatcaagt ttcattatac aaaaaaaagt aagggaacta aagttttgtt tgaaagaact
     6241 gacgctttct ttcatttttc agcttatttt acagtttttc ttaatttcta aatgatttta
     6301 tcaagaaaag gcacggccat atccagccgt accttcttgt tttaagtatg tgaactaaag
     6361 gttctctcca ttttcttcaa tcagtttttg gtaccagtaa taactgtctt tcttatatcg
     6421 ttccaaagat ccttcttgtg tttcgtcttg atcaacatat acaaatccat atcgcttttg
     6481 ataaccgttc agccagctaa gcaaatctgt ataagaccag gtgcaatagc ctaggacttc
     6541 tgctccatcc gaaattgctt ctttgatggc tttgacgtgg ctttgtaagt aattgattcg
     6601 atagtcgtca tggatttgtt tatcttctgt caacttgtca tactctccta agccattttc
     6661 cgtaatcagt accggcatgc ggtaacggct tgtgatacga cgcagtccga tccttaatcc
     6721 ttctgggtca atctcccagt cccaatttgt tcgttcgaca aatggattct caactttttt
     6781 atacattcca ggaactccag tttcttctga tgaacctttt tttcctgtcg tattcatttt
     6841 tccagcacca actccgttta atggattgaa tgcattcgtc gccgtttgat agtaattgat
     6901 cccaagaaaa tctggtttag cagattctag caattctgca tcgccagctt caatcgttgg
     6961 tgcccatcct ttttcttcta agaacctcat ggcagcaatc ggatattttc cccacatata
     7021 gacatccatc caataattgg ccatcaggtc ttctgcattt tcagcagcca gaacattttc
     7081 aggattactg tcaattggat aatttggcgt atacgcaaag cttggtccga tttttccggg
     7141 catttctaac tcgtgaaatt tattgatgac agatgcattt gctaaattag caatatggtt
     7201 aacttcatac attcgtttcg ggtctgtcac acctggcgga tgtgctgcta acaaataacc
     7261 taatgaagtg aagatatttt gttcattcaa actaatccag taatggactt tcccgcggaa
     7321 tgcctcaaat aatgtttctg cataatgagt gaaatcttca atgatctgac gtgattccca
     7381 accattatat tcatcttgca atgcttgtgg aagatcccaa tgatataaag tcaacatagg
     7441 ttcaatatcg ttttcaatca attcgtcgat caatctctca taaaaagcga gcccagcttg
     7501 attgacttct cctcgtccat taggaaaaat ccgtgtccaa gctacagaga agcggtaagc
     7561 tttcagacct tgttctttca ttaaagcaat atcttcttta taacgatgat agtggtctac
     7621 tgccaaatca ccgttcgttc ctttaaatgt ctttccaggt atccgtacaa atcgatccca
     7681 gactgattct cctttcccat cttcttgcca agctccttct acttgatacg ctgcagaagc
     7741 agagccccac agaaaatctt ttggaaatga ggtaagttct ttgtgttcca ttcatctctt
     7801 cctttcctat aaccttcaat cgttcctctt ttagaataac acgaaacccc ttccacaaca
     7861 aaataaaaat atgtaaagga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa
     7921 ttttgtgaaa atggctttct acat
//
LOCUS       DACEOE010000113         7791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEOE010000113 DACEOE010000000
VERSION     DACEOE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.115"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..391
                     /locus_tag="IW774_002010"
     CDS             107..391
                     /locus_tag="IW774_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1154347.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            394..1311
                     /locus_tag="IW774_002011"
     CDS             394..1311
                     /locus_tag="IW774_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1154348.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            1304..2086
                     /locus_tag="IW774_002012"
     CDS             1304..2086
                     /locus_tag="IW774_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1154349.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            2087..2767
                     /locus_tag="IW774_002013"
     CDS             2087..2767
                     /locus_tag="IW774_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1154350.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            2760..3656
                     /locus_tag="IW774_002014"
     CDS             2760..3656
                     /locus_tag="IW774_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1154351.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            3851..5500
                     /locus_tag="IW774_002015"
     CDS             3851..5500
                     /locus_tag="IW774_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1154352.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            6094..7029
                     /locus_tag="IW774_002016"
     CDS             6094..7029
                     /locus_tag="IW774_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154353.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            7022..>7791
                     /locus_tag="IW774_002017"
     CDS             7022..>7791
                     /locus_tag="IW774_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1154354.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         2406 a         1350 c         1718 g         2317 t
ORIGIN      
        1 gaagaaggga ttgtgaaaag tcttttgtca taacctctta tttggtagct aattgtaaat
       61 cccaaacggc atgtattatt tttcataagt ccgtttttaa ggattaatgt tttcttttgc
      121 tttttatatg gtctggcttc agcgagaagt tccaacgcat atacaaggac gtctgatgtc
      181 aacgataaca acgattacca tggtggccag tccacttgga ttagcactgt ttggtgcgct
      241 ctttgattgg cagacaactc aaccacgtct gcgagacagt ctgatttttc ttgaaattca
      301 attggaaaat ttacactttc tttatacatt tctggctgct ttctttatac ccgtcaacta
      361 tacttcactt atcaataaga aagaaggata agtatggatt ttggaaatga aacggtcttg
      421 aaagcaactg gtataaccaa aaaatacggt gctgccaaag cattagataa agtatcgatc
      481 gaaatcaaac gtggaatgat ctatggtttg attggggaga atggagcggg taagtctacc
      541 tttatgagaa ctattatggg tttgatctcg attgatgaag gttcgatcga attatttgga
      601 acgacggatt tgcaggcagc aagacgacgt atgggtcaat cgattgaaac ccctgctctt
      661 tatccagaat taactgcaag agataaccta cgtatccaag cagcgaacgg cggtgtgtcg
      721 gatagagaaa tcgaagacct gctgaaaatg atgcgattgg aaaatacagg gaaaaagaaa
      781 gcaaagaatt tctctttagg tatgcgccag cgtttagcta ttgcaaatgc actgatcacg
      841 aatccagaat ttttgatttt ggatgaaccg acgaatggga tggatccagc agggatggca
      901 gaaatgcgag aaatcatcca acgtttggta aaagaacggg gcatcaccgt cttgctttct
      961 agccacttgc tagatgaatt gtcacagatc gccactcact acggtatttt gcatgaagga
     1021 cacttgatca aagaattatc gaaagaagaa ttggcacaag aatctcgtca atttataaaa
     1081 attgacactt cggcgacgga acaagcagta acggttctcg actctcttgg ttatagggat
     1141 tattttgtcc aatctagccg tgttatccag ctttttgaag gaattgacca agtagcagcc
     1201 atcaatcaag cattggtaga ggcaaaagtt cctgtggatg gtatccacct agttgggcaa
     1261 aaactggaag attatttctt acaattaact ggaggcaatc cacatgttta atctattaac
     1321 tgctgaaaaa ataaaagtat ggaagagccg taaaatgtgg gtctcactag gactgatggt
     1381 cgtacttccg cttttttatt cttggaacga atggtatatg catacaaaat acggcaatga
     1441 attaaaccaa gcaacggata cagtcatcaa cggtgcaaca gggattttaa tggtggaaaa
     1501 aatggctgca tggattcttc tggctttcgc agcatttgcg tgcttttata ttggggaaga
     1561 atttcaaaac ggtacgattc gcaatacgtt atctttagga cggaatcgaa tgacttatta
     1621 tgtgtcgaaa cttctcgtta cgcttttgat cacaacactt ggcgtggttt tgattacggg
     1681 gctagccatg attgcttaca cacttgcttt cggatttggt gaagttgagg gaatcaaaaa
     1741 ctatggaaat tatgttctaa aagtatttcc tgtattgctt ctgctgatat tagcgacatt
     1801 atctgttcca gtggctttga cattcatcac tagaagtacc agtgtcagtt tgcttctttc
     1861 ttttctttat atcatgggaa cggccttcgt tcccggtgtt ttcgcaaaaa tcaaaggctt
     1921 ggaatttcta acggagtggt ttacggaaac gtggctcatg tatacggact ttgcgcaaca
     1981 agcaacttat tcgcaagcac cgaaaatgat tctggtcagt ctagtaacaa tcgtggtatc
     2041 atcagctcta ggaatgttta ttttccaaaa atctgacatt aagtaggtgt tcctgctgga
     2101 aactattttg attatcgaag acgatgaagc tgttcattct ttactggagg aggttcttga
     2161 acagagatac aaacttcttg atgcttattc aggtacggaa ggaaaattat tgttaagcac
     2221 atatcctgtc gatttgatcc ttctagatct catgcttcca gggctttcag gtgaagcatt
     2281 gttagcagaa atccgacaga catcgaatgt tccgatcatc gttctttcag caaaaagcga
     2341 tcaaagagat aaagtttcct tgttggcagc aggagcggat gactatgtga cgaaaccatt
     2401 tgatatcgaa gaactgttgt tacgaatcgg catccaactt cgtcatcaga cttctgtcca
     2461 agtaaaagat ctgtcgcaac gcatctatta caaggaaatc ttactggaca aagagtcgag
     2521 ggatgtaaat gtgaatggtg tggctgtcca tttgacagga agagaattcg atctgctgaa
     2581 gcttttttta gaacatccga aaaaagtatt ttcgcgagca aatctttatg aaacagtttg
     2641 gcaagaacca tattttgata gtgagaaaac agtgaatatg catatttcca atcttcgagg
     2701 gaaactagca aaggcacatg ctgtgtatat tcatacggtg tggggagtgg ggtttagatt
     2761 tgattaagtg gctgttgctt ctcgttgtta tctttatggg aagttttatt ttcttcctgc
     2821 aaagggaaat caagagatta actaagaaaa tcgccactct ttctgaacga caagaatatg
     2881 gtggtcgtct ttcagtcgaa tttcgagaaa agaatctaat ggacctagta gatgcactga
     2941 atacatgggt ggatgaaaat gaacaaaaag gacaagcatt tcaagaaatg gaagaaaaca
     3001 tcagactatc gatcgtcggc ctttctcatg atctcagaac tcctttgact gctattaacg
     3061 gttatgtaca attactggaa caggtagagg atacggagaa acgggagaag tatttaacga
     3121 tcatcaaaca atctgttgca agattactca gtatgacgga tcagttttat gatcttgctc
     3181 gaatagagac gaagcaaaaa gaaatggatc tccatacgct ttctttttct aaagaggtgg
     3241 aagaagtatt tttctctttt tatgaacaat tttctgagaa aaatatcgag gtagcgttcg
     3301 ctgaacactc gataaataca actgtattag cagatccgct catgttgctg cgagtgctgc
     3361 aaaatatcat acagaattta ttgcgttatg cagatggaca agcaaaggtg acattttcaa
     3421 aagaggattc attcattcga ttaacagtgg ccaatcctat caaaccagga gcgcaaacct
     3481 ctgttgaaaa agtcttccag cgtttttata ccgaaaatac cagccgcaca aatacggaat
     3541 ctagcggaat cgggctttac ctgtcaaaaa aattggtgga aggaatggga ggagagatga
     3601 ccgctagatt agacgggggc atcttctcca tctccgtcaa gttgcggagg gtttaattgg
     3661 ctctaattta cctgaagtga atgacaaaaa attgtatctc gttttaaaga catcttcttt
     3721 tttggaaggt gtttttattt tggtgtgatt gatacgaatt aggtaacgaa gacagttatt
     3781 tttgtacccc cttttcaaat agcgcaacag tgctatacta aaactaacaa atcaaggaac
     3841 ggagagggat atgggccaaa gagaatttat cgttttaatt attattgcag ttgttatttt
     3901 attagttgtt ttagacattt tttcacgttt gaaagtaaag gaaacagttc gtagtaattg
     3961 gggaaaaatt ccttatcaac cgagatttga taaagaagaa agtttgaagg atgcgtggct
     4021 cacggagaaa aaattccgtt catgggatag tgaaatcgac gatcttactt ggtatgactt
     4081 ggatatgttc gaagtatttg aagggatcaa cagtacctat tctagtgtag gttcagaagc
     4141 gttgtatcag cgtttacgta gctttgattt tggagaagat tatcagttag aaaaactaat
     4201 tgctttttat caggaaaatc ctcaactgag agaaagaatc caatatcaat tcgctcgttt
     4261 aggaaaaaaa gatcataatt ttgcaaaaca atatctagcg gatggaaaaa gtaaaaagat
     4321 tggacgcgta ggtttattta tttttcttgg cttgcttccg ctggtgggtg taggtttgct
     4381 tcttttaggg caagtactag gaatctatct agcaatcggt ggcttagtat tcaatacggt
     4441 ctattatcta gtgaagaaac aaacactaga aacagagctg aatagcatga gttatttggt
     4501 ttcaacgatt tcagccgcca atcagttagc aaaaatgacg acaccacttc aaaaagagct
     4561 gaaacagcaa actaaaccat taagagacat ccctaaatta ggtttttcat ttcgaatgaa
     4621 gagcgggagt gaagcagaat tgatttttga atatgtcaac atcatgttca tgctgccatt
     4681 tatctcctat catttcgttc tcactaaatt agaaaagcat tcaactgaag ccatcaagct
     4741 gtgggaattg ctgggagaat tggaagttgc agcagccgtt ttgaattatc gcacctatat
     4801 gccgatcact tgccgaccag agtttactga gggcggtgtg acagcgaaag atatctatca
     4861 tccattgtta aaagaagcgg tagttaatcc agtcaactgg cagaagaata cattagtcac
     4921 gggttccaat gcatcaggaa aatctacgta tgtcaaaagt atagccatca gctgtattct
     4981 agcacagacg atccaaacag cgattgcaga acagtttacc ctgcaacctg gacatgtgtt
     5041 gacttccatg gcaatcgaag atgatttatt tgaaggcgac agttattttg tttcagaaat
     5101 caaatcgatc aaacgtttgc tggaccaagt ggcttccaaa gaacgctgct actgcttcgt
     5161 agatgagatt ttaaaaggaa caaatacgat cgaacggatt gcttcttccg cttcagtcgt
     5221 tcggtggctt gcggagtatc catctttagc atttgtcgcc acacatgata tcgaattgac
     5281 agagatttta aagaattatt gtgagaatgt ccactttgaa gaacaagtaa cagaaggaaa
     5341 aggaatcagt tttgatttca aattaaaaga aggaccttca aggacccgaa atgcgattgc
     5401 cttgctgaaa gttttgaatt atcctaaatc catagttgaa caggcccaaa aagaaagttt
     5461 gttgttcgat gaacagcgtc agtggtatcc gtttgattaa ctttatcaaa cgaagcatga
     5521 tgcgtgggtc caaaaagacg attatcaaaa gaagtacatt gatgccaaaa aagtggcaga
     5581 attcaatggg aacgagtctg ctgtccagat gatcttggaa gatttagcaa aagacgatac
     5641 aaaataaacc acgctgtctt ccttctagca gcttttatcg aaaaaactta gcatcagcga
     5701 cgagacgcac gttccatggc aaagttctcc gtaaaacaaa cagcgaaaac agaaattgaa
     5761 aataactatt tccattttat ttatttcggt taaaataaaa gaaaaaagct ttcgataaaa
     5821 ggacggagga aagcttaaca tgaataaaaa agacaagaac ctcagttata aggatcttct
     5881 atatgtcatc gtagtagttg ctgtcggtac ttttatgatt ttaaaagttc cagagttgtt
     5941 tagatagtta ttctatcgaa gatgatcatg aactagaaaa cacatctcct ctttgttagc
     6001 aggtgtgttt ttgtgtggaa gccaagttgt tttttcatct gctgtattcc atgatacaat
     6061 taagaatcat gtctataagg agataggaaa taaatggcaa atgcgcttga aataaataat
     6121 ttgaaaaaga tctatggaag tggggtcgaa gcattgcggg gaatcgatct ttcggtgaaa
     6181 gaaggagatt tctatgcgct attagggcca aatggagctg ggaagtctac aacgatcggg
     6241 attattactt ctttagtcaa taaaacatct ggaaaagtga atgtcttcgg ttatgatctg
     6301 gacaaggatc tagttcgagc aaagcaacag attggattgg ttccacaaga gttcaatttt
     6361 aacccttttg aaacggtcca gcaaatcgtc gtgaatcaag caggctacta cggtgtctct
     6421 cgcaaagaag cgatgaaacg cagcgaaaaa tatttgaagc agtccaatct ttgggaaaag
     6481 agaaatgtcc gtgcacgtat gctttccggg ggaatgaagc gaagactgat gattgctagg
     6541 gcattgatgc atgaaccacg tttgctgatc ttggatgaac caacagcagg agttgacatc
     6601 gagttgagac gcgagatgtg ggagtttcta cgagaattga acgaaaatgg tacaacgatc
     6661 attttgacga cgcattatct agaagaagct gaaatgcttt gtcgcaacat cggaatcatc
     6721 caatcgggtg aactgatcga gaatacaagt atgaaagagc ttctgtcaaa actgcaattt
     6781 gaaacattta tctttgattt agcgccatat gataccaaac ctgtgattga aggatatgct
     6841 tgtacatttg aagatgagca gacaatcgct gtcgaagttg aacgaaatca aggggtgaac
     6901 ggaatcttcg atcagcttac aagacaagga atcaaagttc tttctatgag gaataagtcg
     6961 aatcgtttag aagaactgtt cttgaaaatc acagaagaaa ataatcatac ggaggagaaa
     7021 aatgtttaat ctttatttta ctgcattaaa aagtttagct atcaaggaaa cgaaccgcta
     7081 tttgcgtata tgggtccaaa cactggttcc gccagtgatc acgacatcct tgtattttgt
     7141 tattttcgga aaaatgatcg gtgggcggat cggtgacatg ggtggttttt catatatgga
     7201 attcatcgtt cctggtttga ttatgatgtc tgctatcact agttcgtatg ccaatgtttc
     7261 ttcgtccttc ttctctcaaa aatttcagaa aaatatcgaa gaaatcttgg tagcacctgt
     7321 tcctacgcat gtgattattt ggggctttgt gattggtggt gtgggtcgca gtattctcgt
     7381 cggctctctc gtaacgatca tttccttgtt ctttgtacca cttcatgtgt attcatggtt
     7441 catggtcatc attacattat tgatgacggc aatcttgttt tcattagcag gactgctcaa
     7501 cgggattttt gctcagtctt atgatgatgt atcgatcgtg cctacatttg tcttgcagcc
     7561 gttaacttat ctaggtggcg ttttctatgc catctctatg ttaccgccct tttggcaagc
     7621 tgtttcaaaa gtcaatccga tcgtttatat gatctccggt ttccgttacg gattccttgg
     7681 aacgatcgat gtgccagtag caacatcgat gatcgtactt gtgttattca tcgttgtttt
     7741 gtacgccgtt acttggtatt tgatcaataa aggaagagga ttgagaagtt a
//
LOCUS       DACEOE010000114         7759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEOE010000114 DACEOE010000000
VERSION     DACEOE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.116"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            145..1092
                     /locus_tag="IW774_002018"
     CDS             145..1092
                     /locus_tag="IW774_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1154355.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1104..2024
                     /locus_tag="IW774_002019"
     CDS             1104..2024
                     /locus_tag="IW774_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1154356.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2065..3522
                     /locus_tag="IW774_002020"
     CDS             2065..3522
                     /locus_tag="IW774_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1154357.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3867..4481
                     /locus_tag="IW774_002021"
     CDS             3867..4481
                     /locus_tag="IW774_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1154358.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4474..6198
                     /locus_tag="IW774_002022"
     CDS             4474..6198
                     /locus_tag="IW774_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1154359.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6195..7646
                     /locus_tag="IW774_002023"
     CDS             6195..7646
                     /locus_tag="IW774_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1154360.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2618 a         1289 c         1543 g         2309 t
ORIGIN      
        1 gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt
       61 tcattttccc tatactttgt ttatttattc tcattattga aagcgctata attacgttgc
      121 gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg
      181 aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt
      241 tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca
      301 gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa
      361 tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta
      421 gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg
      481 agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct
      541 tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat
      601 cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg
      661 ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac
      721 tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct
      781 agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt
      841 ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt
      901 cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat
      961 ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt
     1021 gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct
     1081 gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct
     1141 ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct
     1201 gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga
     1261 ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg
     1321 accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag
     1381 gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact
     1441 ttccattccg tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga
     1501 tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat
     1561 tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac
     1621 gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa
     1681 tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg
     1741 ccgcattggg ttactggaat gactggttca atgctttact atacatccaa gatgataagc
     1801 tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa
     1861 attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca
     1921 gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga
     1981 agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa
     2041 atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt
     2101 tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca
     2161 gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc
     2221 gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat
     2281 atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg
     2341 gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt
     2401 gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa
     2461 gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat
     2521 gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata cgatctttca
     2581 attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa
     2641 gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat
     2701 tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc
     2761 atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac
     2821 aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga
     2881 aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg
     2941 acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca
     3001 gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca
     3061 gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg
     3121 atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat
     3181 ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat
     3241 acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca
     3301 gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt
     3361 aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca
     3421 gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg
     3481 aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag
     3541 gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca
     3601 gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt
     3661 tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag
     3721 ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt
     3781 tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta
     3841 taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc
     3901 ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga
     3961 tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat
     4021 tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa
     4081 gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat
     4141 atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg
     4201 ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga
     4261 tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt
     4321 ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat
     4381 tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt
     4441 ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat tctattttca
     4501 aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg
     4561 atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa
     4621 aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg
     4681 agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc
     4741 tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc
     4801 gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta
     4861 agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac
     4921 aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg
     4981 cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa
     5041 ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct
     5101 ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa
     5161 agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat
     5221 ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct
     5281 gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt
     5341 ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat
     5401 tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag
     5461 acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac
     5521 gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag
     5581 caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca
     5641 ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg
     5701 tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa
     5761 gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga
     5821 gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg
     5881 atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat
     5941 gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat
     6001 ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa
     6061 gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc
     6121 ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt
     6181 acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt
     6241 tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag
     6301 cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa
     6361 cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac
     6421 gtcataattt tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag
     6481 gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg
     6541 aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag
     6601 aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg
     6661 aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg
     6721 caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact
     6781 atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg
     6841 atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg
     6901 gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc
     6961 tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag
     7021 aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat
     7081 tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga
     7141 tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga
     7201 ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg
     7261 tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt
     7321 acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta
     7381 cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca
     7441 aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga
     7501 aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca
     7561 acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt
     7621 accgagaaaa cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa
     7681 aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc
     7741 atgacctctc gataaacgg
//
LOCUS       DACEOE010000115         7739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEOE010000115 DACEOE010000000
VERSION     DACEOE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.117"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..868
                     /locus_tag="IW774_002024"
     CDS             <1..868
                     /locus_tag="IW774_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154361.1"
                     /translation="DKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKVVVTVRKRQT
                     KKRSTFHVSPDIYNLIERHRSEDNISISQYIGTVIDELISGKYDYAFDFENEKDYRSV
                     AVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSHKLSQALKEV
                     TEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMNELSYEHLTV
                     HEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDK
                     YKENSDKFSKDK"
     gene            888..2522
                     /locus_tag="IW774_002025"
     CDS             888..2522
                     /locus_tag="IW774_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154362.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNNGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMSLTIKRIYDLLYVYKH"
     gene            2494..3720
                     /locus_tag="IW774_002026"
     CDS             2494..3720
                     /locus_tag="IW774_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154363.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRSKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKRYNELEFKEGNEL
                     IVNFADLCSGECLVNNKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     repeat_region   3833..4050
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3833..3855
                     /rpt_unit_seq="tttagaaccatataaaacagcat"
     gene            4237..5562
                     /locus_tag="IW774_002027"
     CDS             4237..5562
                     /locus_tag="IW774_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1154364.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWSYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            5555..5902
                     /locus_tag="IW774_002028"
     CDS             5555..5902
                     /locus_tag="IW774_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154365.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
     gene            6253..7074
                     /locus_tag="IW774_002029"
     CDS             6253..7074
                     /locus_tag="IW774_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1154366.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            7094..7654
                     /locus_tag="IW774_002030"
     CDS             7094..7654
                     /locus_tag="IW774_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAQ1154367.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT         3037 a          939 c         1459 g         2304 t
ORIGIN      
        1 agataaaatt gtagatgaaa ttccggcaaa aagtcgagaa caagctagaa aattatttat
       61 tgaaaaatac aaagaaatca atggagatag tgaagttcca aaagtggtcg taacagttag
      121 aaaaaggcaa acaaaaaaga gaagtacgtt tcatgtcagc cctgatatat ataatcttat
      181 agagcgacat agaagtgaag ataatatttc tatatctcaa tatattggta cagttattga
      241 tgaattgatt tcaggaaaat atgactatgc ttttgatttt gagaatgaaa aggattaccg
      301 aagtgttgct gtctttcaat ctgattttga gaagctaaaa aactatgctg gcaggagaaa
      361 tgttaatttt gcaagactac tagaagagtt ctactccgaa gagttgggag taggaaatga
      421 atacaagaga gaaacttatg cgaaattgtc tcataagtta tcccaagcgc ttaaagaagt
      481 gacagaagtt aaagcggaac tagatgctaa acttgaagaa gtgaaaaaat atcaaacaga
      541 gaggaagaac aacatgaaga atcaagtgga atttgacgaa cagaaagaat ttgaaaaagc
      601 aagaaaacgc tataacaaaa ttatgaatga acttagctat gaacacttaa cagttcatga
      661 agaattgagt gagggtacag aaaaatatac gatcgcagat atggttaatg aagtggaaac
      721 tcttttagct tctttctttg aaaatggtca cgtaaacgat gaatggcgaa aagaagaacc
      781 taaaagggtt aacgcttata ttgctcgatt aaaaaggttt attgataaat ataaggagaa
      841 tagtgacaaa ttttctaaag ataaataagg agagagtgtg attacatttg ccagaaatcg
      901 aaggaatatt ttcgccaaaa ataggtaaaa aacagttaga tttcagcagt tttccgagcg
      961 taaatagaga ttataatcta ggtgatgagg tacagatact aaatattgat ttcaaagaac
     1021 tagacttcgc tgttgctgtt tccaacaaac ccaaggcgtt ggaacgagtg aaaaaaatat
     1081 accaagagaa tgatcaaaat atcaaaaata tgatcaaaaa gtatagacga gataattcaa
     1141 aagttgaaga cgttttattc aacggtacgg caaaacatga tttgaatgtt tatatggcat
     1201 taggagtggc tttgtactgt aattcaatta atcagacaga tattttggtt aatatcgtga
     1261 ggaatagcta tcaaaaaata ttaaaaataa gtagcgaaac aaacccttat tatgcaatga
     1321 aatatcttgg ggataattta ttaaatgata aaaaattgat gcacaacact gaagagctgt
     1381 tatctaattt ggctgcgatt aactttctat tcaaaacagg ttctgaagat tattttatag
     1441 ataacgtact aaaaggtgct tacggaacag cattttatcc gctttacaat atgaaacagc
     1501 ataaagccga acaaaaggat aacaaacttg aaattaaaaa gatgaaagaa ctattaggta
     1561 taggatttgt aaagcccaat tcaaaagtca ctattgcttc gttagcaact actaacaata
     1621 atgggaaaat agtaaatggg tttgacgatt cagatataga tatattgtca aaatcatatt
     1681 tttctttgta tggagatttt gcagatttga tgggtattga tagttactta gtcaataatt
     1741 ttgagatcaa gggtagcgag ctgaatgata ttgtgtattt cgttttaatc tctctatatg
     1801 caattgatca gaataccgag taccgacaaa ttgactattt gctagttttt tatagtttgt
     1861 tatatcagtt cgttttaact cggttgttta aaaatgctaa ggaagaattg atcctgaatt
     1921 ctgattatga aatagcaaaa caaattgcaa gtattagaaa agaaaacaaa tctgaagtaa
     1981 tcaaattaag aaaagaatta agtattgcga gtaaagaaaa cggtcggtta gaatctttag
     2041 tcaaatcggc aaaaaaagaa gaggaacttt tgaagcaaga gattaaagaa ttaaaaggaa
     2101 tcagaaaaga acttgaacag ctgaggtttg aaaataaagt attaaagcaa ttgcaggagc
     2161 aagaacatag acaatcatta aatggtaaaa ctgaagagcc ttcactgtct gaaatgatag
     2221 cttatctaaa acataaaaac attcttttag tgggaggaca tgtaaatttt caaaataaat
     2281 taactgaact attaccaaat attaaaatgt ttgatgtaag cgaaaataat ggaaagatac
     2341 ttaaaaattt gaatacgtgt gatgcgattt ttctatatac agattacttg aatcatggtc
     2401 tatattatcg tgttatgagt gaaatttcta ataaagataa gatattttat ttagataatc
     2461 atgtgaatat gagcttaaca attaaacgta tttatgatct tttatatgtt tataaacatt
     2521 aatgaggtgt taaaattgac aacaataact atgacaaaaa aaatagaagt atatgttgat
     2581 ggatcacaag aagaaaggga ttcctttttt gatttttttt ggaaaaatag tagagaatat
     2641 gtaaaagcgt tgaatctggt atataaacag ctaatgtttg aagagttgat aaaagatgca
     2701 attaaagaaa tggatcaagg ttatgctgag agagaagaaa gtattcaaaa tcggattgaa
     2761 aagttagcat atactgatcc aatagatcaa aaaaaacttg ataaagcaaa agctgaatta
     2821 tctaaattga gaaatgaaaa aaataaagaa gcgagagaaa tattaacgca agcgataggg
     2881 ttgaagagtc aaacgagaac acgagatgta gcaaaaaaat tagactttga ctttggagat
     2941 tttgttgatt ccgcaaatat gaaagctagt aaagattttg gcaatgattt tatcggaatt
     3001 gtgaccggac aaagaacgcc tagaatatat aaaaatagaa gtaaatatgg attattacct
     3061 tttagaggtc gagatattaa aatccataaa gatgataagt tttatattta tttacctaaa
     3121 ggctttaggt ttaaagtgaa tttaggtagc aagccaagaa aagcaagaga tgttgcttca
     3181 gttttacaac acattatgga tggcgattac aaattaaatc aaagtaatct tatttttgat
     3241 ggaagaaaat tattttttaa tctaacaatt tcttttgaaa aaagatataa tgaactggag
     3301 ttcaaggaag gtaatgaatt gatcgtaaac tttgcagatt tgtgtagcgg tgaatgttta
     3361 gttaataata aatcggtaat gtcttttggt gatagccaat atataagaaa cttcaaaagt
     3421 aaaattttag caatgaaatc aaaagaacaa agccgaggag tcttttctaa aggcggtcat
     3481 ggtcgaaaaa gaaaattaga tcaagatcga tgggaagcaa taaagaaaag agaaagtaat
     3541 tttgttaaaa cttataataa tcaatgttct gcaaaaatcg ttaaaactgc tattcgcaat
     3601 aaagctcaaa ttattcgtat cattgaacca gataaggctt atggcgaatg ggcttactat
     3661 caattgaaag aacaactagt aaataaagga aaaagatatg ggatagaagt cagaagttag
     3721 taaagtaaac aatcgttgat ttggcaacga aatagtgagg gaaaatccac tcactgaagg
     3781 gtaaactccg tcaagttgtg tgaccagacg gaaggacgag cgttgttggg gttttagaac
     3841 catataaaac agcatgtttc tcaaattgtg tttgtttcat ttatgttata ataagagttt
     3901 tagaaccata taaaacagca tgtttctcaa atgagtactt taaattaatt aatggcgttc
     3961 ctgctttaga accatataaa acagcatagt tctcaaattg tttaaaatga ttgtcaagtt
     4021 aaaaaaatct gggatcatat aaaatagtat agttctcaaa tatgattaga aaccctatta
     4081 aagagcaata ttaagccaac ttacttaaat cgtttaatta agaacagtaa tctcttttag
     4141 tcagactttt agtctgactt ttttgtattt ccaaaattta ttctttacaa aaacgaacat
     4201 atgtttgtat aattgcttgt gaatggggga ataaaaatgt ttgattacag tagagaaccg
     4261 gtgaatgact attttttaat cgacatgaag tctttctatg ctagcgtgga atgtattgaa
     4321 cgaaatttag acccaatgac tacaccgtta attgtcatga gtagatcgga caacactggt
     4381 tcaggtctga ctctagcttc ttcacctact gcaaagaaaa aatatcatat tactaatgtt
     4441 agtagagcta ctgatttacc ttatccatta ccaaaagaac ttcatattgt tccaccacga
     4501 atgaacttat atataaaaaa aaatatggaa gttaatcaga tttttagaaa gtatgtcgca
     4561 gatgaagatt tattgattta ttcgattgat gaatcaattt taaaagtgac aaaatcctta
     4621 aatttattta ctagttcagg aacaagaagc gagagaagaa gagaattagc taaaataatc
     4681 aaagatgaga ttcagaaaga aacaggtcta atagcaacag tcggaatagg agataaccca
     4741 ttattagcta aactcgcact ggacaatgaa gcgaagagta gtaaagaatt tatagctgag
     4801 tggtcttatg agactgtacc tgaaaaaata tggaaaataa acgagatgac tgacttttgg
     4861 ggcattggat cacgcatgaa aaaaagattg aatcatatgg gaattatgag tatagaagat
     4921 ttagcaaatt ggaatcccta cacaattaaa agtcagttag gtgtaatcgg acttcagtta
     4981 tatttccata ccaatggaat tgatcgtacg gatattgcta ttccaccaga accaaccaaa
     5041 gaaaagtctt atgggaattc acaagttcta ccaaaagatt atacgaggag agaagaagta
     5101 gaactagtag tcaaggagat ggcagagcaa gtagccattc gaattagaca acacaattgc
     5161 aaaactggtt gtgtacgctt agcaattggt acgtcaattt tggaagaaaa atcaggtttc
     5221 tcgcaccaga tgaaaatacc aattacagat aatacgaaag aattacaaaa ttattgtctt
     5281 tttttattca ataagtttta tgaggggcag gaagtacgac atataggagt aacttatagc
     5341 agattagtat acacagattc cctacaatta gatttattta gtgatccaag aaaaaagatt
     5401 gctgaagaaa atttggatag gattattgat aaaattagac aaaaatatgg atttacagct
     5461 attatacatg cttcatctat gttagaaggt gcaagaagta ttacgagaag cactttaata
     5521 ggaggacacg caggaggatt aggaggtttg cataatgatt gaatataacg atagaggacg
     5581 tttaaaatgg tcgggatttt atttgtctga tcatactgaa agaatcaatg cagacaatgt
     5641 acaacgtgct aatcataatt tggcaaaaga acaaatgacg actgaagaga ttgcgaaaat
     5701 actgagaact gctgtcataa aaaatagacc cgtctgcatt caaaaagagg aacgaaacgc
     5761 tgaaggttac tatccagatg atgtagtagg aaaaattatt ggatatgacg agcttggtgt
     5821 atttgtggaa gaagaaaaag ttcactatga tgaaattagg aatgtttctt tcgcggaatt
     5881 attaaaatgg agtcagatat gaacattatt aaaaaatacg aattcggtta tattacgtat
     5941 gaacagctaa tagaggaatt atggggatat ggtcaacaat taattaatga agtggggatt
     6001 gattgctttt gtttctatat tgaatccgga gcaggatacc ataagaacag atactatata
     6061 tttccatatc caccaaaata gtctcttaga gatttttaaa ttttctccaa atactttcaa
     6121 ttattgtgaa atatttcctt ttaattaatc aaatttttta taaaagtaat atataaaata
     6181 aattgcttga agtattatat ttagatatca taatttatgg aagaaaaatt aattaaaaag
     6241 gatgagatta taatggggtt actggttccg agagctaata cttatactat acctttaacg
     6301 aaacgccaat taattgctaa aagatatcaa aggattacta gagccatcaa tagagagttt
     6361 tggaattctg aaagtgatac tgctcacagt ttatacgtag gttcttatgg gagaggaacg
     6421 gcaattagta ctagtgatat agatataata gttgaattac cgatggcaga atttgacaga
     6481 ttcaaaaatt atttatcaaa tggcccgtct aagttattac aagttataaa aaacgcattt
     6541 caggaaatac taccaaatag tgatattaga gctgatggac aagttgttaa aataaatttc
     6601 catgatggaa tcaaatttga aatagttcca gcttttaatg aaaaagatta ttggggtgag
     6661 tcaaaagggt ttatttatcc agattcaaac atgggaggga actggaaagc tacaaatccc
     6721 aaaaaagagc aagaagccat gaaattaaag aatacaaaaa gtaataattt attatatgct
     6781 acgtgtaaac attttcgaca tgtacgagat acagaattta cgagttatca tctttcagga
     6841 atagtaattg acagtttcgt atatgaggct atgggaaatt ggaaattcgt tgaaaataac
     6901 agtgggggtc aaaacattag ttctgtatct tatgagacag ctttattaga atactataac
     6961 tcacataaag ttatgggcgg tttaaattta tactctcctg gaagcaatca atttgttaat
     7021 tctgatagta gtatcatatg tttagaaaaa gtattaaaaa aaatagcttt ataaagtttt
     7081 atggaggaac aacatgggag acgaaaaaaa gcgcttggaa gttcaattac gtgaagcata
     7141 tggacgagta acgtatactt acaccactca tttaaagcaa gccgataata ttatttctga
     7201 caataaaatt ttaaggtgtc ttcaaattgg tttgtcagcc cttactactg gaggggtact
     7261 tagtaccatt attactgata atagtgagtt aacctttgtt gctggattat gttcaacaat
     7321 ttctcttgga ttaaacttat actttaagaa ctttgatctt gctgaagaag ctaaaaatca
     7381 tcaaattgtt gccgataaat tatggttgat cagagaagaa tacgtaagct tactaacaga
     7441 tttaaatcgt ttaagtgttg aagatattgt gaaaaaaaga gatcaattaa tgaacgaaac
     7501 tcatgaagca tataaaaatg cccctaaaac aaatagtaaa agctatgaat cagctcagaa
     7561 agcattaaaa tctgaagaag agcaattttt tacagaagag gaattggata agatgttacc
     7621 agagcatctt agaaaaaaga gttctaaata ttgaactctt tttttatgct taaaattctc
     7681 ttgtaatttt tctattcaat caataaataa tctatattta agaagaaata tgatgatag
//
LOCUS       DACEOE010000116         7701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEOE010000116 DACEOE010000000
VERSION     DACEOE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.118"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..970
                     /locus_tag="IW774_002031"
     CDS             107..970
                     /locus_tag="IW774_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1154368.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IW774_002032"
     CDS             complement(1099..2721)
                     /locus_tag="IW774_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1154369.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IW774_002033"
     CDS             2970..3491
                     /locus_tag="IW774_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154370.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW774_002034"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW774_002034"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1154371.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IW774_002035"
     CDS             5572..6390
                     /locus_tag="IW774_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1154372.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IW774_002036"
     CDS             complement(6493..6690)
                     /locus_tag="IW774_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154373.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IW774_002037"
     CDS             6859..7437
                     /locus_tag="IW774_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1154374.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         2300 a         1381 c         1532 g         2488 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat t
//
LOCUS       DACEOE010000117         7651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEOE010000117 DACEOE010000000
VERSION     DACEOE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.119"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            316..1854
                     /locus_tag="IW774_002038"
     CDS             316..1854
                     /locus_tag="IW774_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1154375.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1851..2816
                     /locus_tag="IW774_002039"
     CDS             1851..2816
                     /locus_tag="IW774_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154376.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2803..4125
                     /locus_tag="IW774_002040"
     CDS             2803..4125
                     /locus_tag="IW774_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1154377.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4188..4292
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4366..5109
                     /locus_tag="IW774_002041"
     CDS             4366..5109
                     /locus_tag="IW774_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1154378.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5124..6116
                     /locus_tag="IW774_002042"
     CDS             5124..6116
                     /locus_tag="IW774_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1154379.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6128..6865
                     /locus_tag="IW774_002043"
     CDS             6128..6865
                     /locus_tag="IW774_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154380.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6855..7577
                     /locus_tag="IW774_002044"
     CDS             6855..7577
                     /locus_tag="IW774_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1154381.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2492 a         1313 c         1655 g         2191 t
ORIGIN      
        1 ccaataaagg tgttctcccc cacgagattt ctactttatt ggctgtttct ttttattaaa
       61 ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa gtgacaattt cgtatagatt
      121 tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt gatccgactt tgttgcttgg
      181 attctttttt tgtcagcata tttagaggta tttttttctt ttttttgttc gtgctgtaag
      241 gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat gtttatactt aaagaataac
      301 agaaaggaag gtcgcatgaa taattttata caaacattaa gtgaaagaaa aggcgagttg
      361 ctggaagctg catttcaaca tctgtccatt tctctcattt ctttgctgat tgcagcactg
      421 attgctattc ctttagcgat ttgggcagcc aatcataaaa aaatggcaga agtcttattg
      481 cagatcacgg gtgtgctcca aacgattcct tcacttgcct tattaggttt acttatccca
      541 tttgtaggga tcggaactgt tccagcattg attgcacttg ttatttacgc tttgcttccg
      601 attttccaaa acacgtatat tggtctagct gaaattgacc cgtctattga agaagcagcg
      661 gtagcatttg gtatgtccag aatgagacgc ttgctgaaag tagagcttcc catcgcatta
      721 cctgtcatta tctcaggtat ccgaactgcc ttagttttaa tcatcggtac ggctactttg
      781 gcagcattga tcggagcagg tggattaggg acatttattt tgttaggtat tgaccgaaat
      841 acacctgttt tgacattgat tggtgcgatc agttctgcct tgcttgcaat tatattcagc
      901 ggactgattc gctggttgca gcataagaaa cctcgatatt ccatcattac tttagcagca
      961 gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg ccttgtttaa aaatgaaacc
     1021 atcacgattg ctggaaaatt aggagcggaa cctgaaatcc tgatccaaat gtataaagaa
     1081 ttgattgaag acaacacaaa aacagaagtt gaattaaaac cgaattttgg caagacgagt
     1141 tttttgttca atgcactgga tcaaggtcaa atcgatatct atcctgagtt cacaggtact
     1201 gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa gtgctgattt gacaaaagaa
     1261 aaaacatatg acgaagcaga agaattattg aataagcaat tcgatatgac attattaaaa
     1321 ccaatggctt accaaaatac gtatgccttg gcagtcaaga gatcttttgc gaacgaacat
     1381 caacttaaaa agatttccga tttggcagct atctctgatc agatcaaagc gggattcaca
     1441 ctggaattta ttgatcgttc agatggatat aaaggaatcc aagaagcata tggcttgaat
     1501 ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc aagcaatcaa taatggagat
     1561 gtcaatattg tggatgccta ttccacagat agccagctca aggagtacga tctagtgact
     1621 ctagaagatg atcgagggtt attcccagct tatcaaggcg caccgctgat gaaaaaagat
     1681 tttgctaaag aacatcctga aatcgttaaa gcactgaata aattaagcgg aaaaataaca
     1741 gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg aaaagaaaaa gccgacagaa
     1801 gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg aggaagatca gtgactaaat
     1861 taatcgagtt caaacatgtg caaaaaaagt atgatggaaa gtatgtcatc gatgacctaa
     1921 atctggcaat caaaaaagga gaaatctttg ttttggttgg tccttccggt agtggaaaaa
     1981 caactacttt gaaaatgatc aatggattat ctaaaccatc agcaggagac atctacttca
     2041 aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg gtggaatatg ggatatgtgc
     2101 tgcagcaaat cgctttgttc ccgaccatga cagtcaagca aaatattgaa gtcatccctg
     2161 aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt agatgaactt cttcaaaaag
     2221 ttggcttatc tcctgatatt tatcgagatc gtatgcctag agagttgtct ggaggagaac
     2281 agcaacggat cgggatcatc cgagctattg ctgcaagccc agatgtgatt ttgatggacg
     2341 agccatttag cgcattagat ccgatttcaa gaaactcatt acaagaattg gttttgtcct
     2401 tacacgaaga attgggcacg accatcgttt tcgttacgca caatatggaa gaagcaatca
     2461 agctaggtga tcgtattgca tttatgaagg atggagaaat tatccaatgt gacacaccag
     2521 agcagttatt gatgaatcct aaaaacgact acgttcggca tttctttgat gaaccgaaac
     2581 agacgaaaga gtggcgagta gaagatttag tcgtcaatgg atatttctta aatgaaatac
     2641 cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat gaaagaagta tattcgcttc
     2701 tttcggtgta tccaagcatt gtgatagtag aaaagcaaag aagtattggg tcgctgacaa
     2761 gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg aaatgaaaac atatgatgca
     2821 atcatcatcg gttcaggtgt gagcggctta agtgctgctt atggattgaa agaagcaggt
     2881 aaaacagtac tagtggtcga agaagatcta tggggcggga cttgtcctaa ccgtggatgt
     2941 gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta atcgggtaaa acaattgagt
     3001 ggaaaaggtt ttaacgagat cccaactgcc aactgggaag aacttcaaaa attcaaacga
     3061 acatttacgg atccagtccc agaaagccga aaaaaacaat tagcagaagc tgaaatcgat
     3121 catctttcag gaaccgctcg ttttttggat gattcatcta ttgaagtcaa cgaagaagtc
     3181 ttccatgcgg attacttagt tttggcaacc gggcaaagac ctacgatctt accagtggag
     3241 ggaaaggaat acttgaaaac tagtgcagac tttttatcat tgcctgtact gcctaaagaa
     3301 atcattttta tcggtggtgg ttatattgct tttgagctag ctacgattgc caatgctgca
     3361 gggagcaaag taaccattgt ccatcataat caacggccat taaaagaatt tgaggcgtct
     3421 ttggtagagg aagccgtcca tcaaatggaa gcatctggca ttcagtttgc ttttggtgtt
     3481 gaaacccaaa agatcatttc agaaggtact cgctatcgtt tagtcggaaa agagacggaa
     3541 ttagtagcag atatgatttt ttgtgcgact gggagacagc cgaatacaga atctttagca
     3601 cttgagcaag cgaatatcgt ctttgataaa cacggaattg cagtcaatga ttatctgcaa
     3661 acgagcaatc caaaaatttt tgcgtgtgga gatattgttt caaggaaaac cccaaaattg
     3721 acaccagtcg ccacttttga aggaaattat gtagctaagc ggataacgga tgcaacaagc
     3781 gaacctatca aatatccgat tatacctacg atcgtctacg ccagtcctaa acttgctgaa
     3841 gtaggagtta caaaaagtca cgcctcttcg tcagatcaag tggtagagat ggatcttacc
     3901 agttggttca cctatcatcg tgtcaatgaa ccagttgcta aggctgaatt gacctttgac
     3961 cagcaaaatt atttgattgg tgctgcagtg attagcgagc aagcagacga attgatcgat
     4021 gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag agttggatag ctatatcatg
     4081 gggtatccga cattggcaag tgatctctcc tatttattga aataatcagc atgataacta
     4141 aagcgcttgt tgctttttcc ttcttgtgct acactttaaa aaatgaatac ctgtttctgg
     4201 ggatgtcgta gacgacatga gagtaagttg tgaacttaga cccattgaac ctgtcagtta
     4261 atactggtga agggagaaac atagcagacg cttgaagcgg gttactatgt cgtgcccatt
     4321 tcaagcgttt tttgttattc atattagaag aaaggaggta acaaaatgaa gaaaaatctt
     4381 tatccagcag tcagtatggc attcatttta attatttggc agattgttgt catagtttgg
     4441 aaaatacctg cctttatttt acccggacct gctgctgtat tttctgcatt agcggcagat
     4501 gcacaagtat tactttccca ttctgtcacg acattaaatg aagcagtgct tggtttggta
     4561 atagctagcg ttctttcttt tattactgcc ttggcaatgg attattggaa gtttttagaa
     4621 atgtcttttt atccgttact agtgatttca caaatgttgc caatcatggt gttagggcca
     4681 ttacttaccc tatggtttgg tttcggtatg cttccaaaag tgatccttgt tgtcttgata
     4741 agctattttc cgatcgtagt ttctttttcg gatacattga ggaaagtatc aaaagagcaa
     4801 atcatctttt tgaaaacgat gggggcagat acagggaaga tttatcgaat atataaaata
     4861 ccagcgggtt tatccggctt cttttccggt ttaaaagtgg ctgctactta ttgtgtcagc
     4921 ggagcagtcg ttggagaatg gctaagcgct caagcaggct tagggtatta catgatacgc
     4981 gtaaaaaaca gctaccagat cgataaagtt tttgctgcga tcatttgtgt cattctattg
     5041 agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag ggtattatcg tatgctttat
     5101 attttttaag agaaggggat caaatgaaaa aattatggct tttattaccg ctactgttgc
     5161 ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa acaagaaata aaggatttga
     5221 aaaaagtgac acttgttttg gattatgtac cgaatactaa tcatactggc atctacctag
     5281 caaaagaaaa aggctattat aaagaggctg gtttaaatgt ccaaatcatc gaaccaggag
     5341 ataattcgac aagtattggt ctagtaggtg cggacaaagc acaatttggt gtaagttatc
     5401 aagaagatgt cacttatgct cacgcagatg gacagaatat accagtcaaa gcaattgcta
     5461 cagtgatcaa acataatact tctggattcg ccaccctatc agacagtaac atccattcac
     5521 caaaagattt tgaaggaaaa acatatgcag gttggcaaag tccaagtgaa gaagctgtct
     5581 taaaagctgt gatggaaaaa gacggcgggg acttttcaaa actgacaatg gtaggaagca
     5641 atggagaagg gccagaaagc ttaggaaaat cctcagatat ccaatggtac tttgaaggtt
     5701 gggatatgat caaagcaaaa gaagcgggta ttgaactaaa ctatatccca ttgaaagaat
     5761 tagatgaacg gttagattac tatactccgg taattattac gaatgatcaa ctgatcaaaa
     5821 gtgatccaga attggtacag tcttttatgg atgccacgaa aaaaggctat caggaagcta
     5881 ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata tgcaaaagaa aatgatcgga
     5941 cgtttttaga agaatcacaa gcattcttgt caaaaaatta tacggatgat cctgagaatt
     6001 ggggattgat ggaagaaaaa gtttggcgca attatacagc ttttatgcag gaaaacggat
     6061 tgatcaagca agatgtacct tcgcaagaac tattcactaa tcagtttata aaataggagt
     6121 gatagatatg tcaatccaaa tcagccactt gaatgtagtc attgaaaacc tgaaaatatt
     6181 agaagacatc catttcacta tccctaaaga tacctttaca tcgattgtcg cgccaagcgg
     6241 agccggtaag tcaacattgc tgaaaacttt aacaggagtc cagcctatga cttctggaac
     6301 gatcaaagta gacgatcaaa aagtaactgg tctccataca gcatttagtt atatgccgca
     6361 agaagatatg ttacttcctt ggctgagcgt ctatcaaaac gttacgttat atcaaaaaat
     6421 caatcatcta acaatcaatg aagaacaagt aagggattat ctggatatct ttgggttaaa
     6481 cggctatgaa cattttttgc cggaacaatt atctggcggg atgaagcagc gaacggcctt
     6541 gttacgaaca atcatgaatc cttcttctta cttgcttctt gatgaaccat ttggagcgtt
     6601 agatgctatg accagagggc agatgcaaga ctggatgttg aagctgccta aaaaagcaaa
     6661 gcggactact ttacttgtga cgcatgatat cgaagaagca atttatttat cagacaggat
     6721 tcttgtgctt tccgcccgtc cggcacatgt gattgctgag attcaagtgc ctgaaaaacg
     6781 acggtcacgt gaatggctac tgcagcaatc agagttgaag caaacgattt atcagttatt
     6841 ggcaggtgaa ttagatgtta agtgatgctc attgtcattt agatgggagt cagagtttag
     6901 cactccttca gcaggaacac acgattttga cgatcatcaa ttgtgattcg ccagaagaat
     6961 ggaaggaaaa cagacagctt gcagcaagta agactcaagc cttaagttac ggtatccatc
     7021 cttggaaagc agatagctat acgtttgaac aagttgagcc ttttttgaaa aaagcaagga
     7081 tcatcggtga aatagggtta gataatattt ggacgaatgt accgatgact acacaaaaaa
     7141 aagtatttga gcgacagctt gcttttgcag ccataaatga gaaacctgtt gtactgcaca
     7201 caaaaggatg cgaaaaagag atcttaaatt atatccaagc ttaccctaat cactatttga
     7261 tccattggta ttcttctttg gactatcaac aagactatat tgatttaggc tgttatttca
     7321 gcattggtct tgatttgaaa aagaatccag cagtctggca actagctaaa gcagtcccaa
     7381 tcgaccactt acttattgaa acggatggac tacatgcagc agagtgggct ttagggggga
     7441 ttttttcaga aaaagattat gtctcactgc ttgaagaaca gttaaagaca atcgctgaga
     7501 tcaaagagct atcagttgaa gaagttaaaa aaaatactta tcaaaacttg gaacgtttca
     7561 ttagattagg taaataaaaa agagatgtga cacaactagt gtcgcacctc tttttggctt
     7621 tctttgtata aaagtaaact gtttgattaa t
//
LOCUS       DACEOE010000118         7645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEOE010000118 DACEOE010000000
VERSION     DACEOE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.120"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(46..396)
                     /locus_tag="IW774_002045"
     CDS             complement(46..396)
                     /locus_tag="IW774_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154382.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(389..1714)
                     /locus_tag="IW774_002046"
     CDS             complement(389..1714)
                     /locus_tag="IW774_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1154383.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            complement(1883..2467)
                     /locus_tag="IW774_002047"
     CDS             complement(1883..2467)
                     /locus_tag="IW774_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1154384.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(2678..2881)
                     /locus_tag="IW774_002048"
     CDS             complement(2678..2881)
                     /locus_tag="IW774_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154385.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(2891..3313)
                     /locus_tag="IW774_002049"
     CDS             complement(2891..3313)
                     /locus_tag="IW774_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154386.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            3627..4241
                     /locus_tag="IW774_002050"
     CDS             3627..4241
                     /locus_tag="IW774_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1154387.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(4406..5410)
                     /locus_tag="IW774_002051"
     CDS             complement(4406..5410)
                     /locus_tag="IW774_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154388.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(5869..6126)
                     /locus_tag="IW774_002052"
     CDS             complement(5869..6126)
                     /locus_tag="IW774_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1154389.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(6119..6388)
                     /locus_tag="IW774_002053"
     CDS             complement(6119..6388)
                     /locus_tag="IW774_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1154390.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(6519..6698)
                     /locus_tag="IW774_002054"
     CDS             complement(6519..6698)
                     /locus_tag="IW774_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154391.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(7079..>7645)
                     /locus_tag="IW774_002055"
     CDS             complement(7079..>7645)
                     /locus_tag="IW774_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154392.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         2341 a         1455 c         1107 g         2742 t
ORIGIN      
        1 gctgatcaaa ggttatatac tttagctcat attgctggat aatgttcatt ctaatcgact
       61 ccatttatta tcctggtaaa attcgatatg acgtatttcg tcgtagtgaa ttttgtcttt
      121 tccaacgtag atccccagct catcactacc taagatcttg ccaatgatat cttctggata
      181 gtatccttca gcgtttcgtt cttctttttg aatagatact gatctatttt ttatgactgc
      241 agcgttcaat acttccgcaa tctcttcagt cgtcatttgt tcttttgcta aattttgact
      301 ggttcgttgt tcattgtcgg catcaatttc ttcagtatga tcagataaat aaaaccctgc
      361 ccactttaaa cgtccacgat cattgtaatc aatcattttt aattccccca tttccgcccg
      421 catgaccacc aacaagtgtg cttctagtaa tgcttctagc gccttctaac attgaagaag
      481 catgtacaat cgacgtaaaa ccatattttt gccgaatctt gtcaataatt ttatctaaat
      541 tttcttcgtc aatttgcttt tgtggatcac taaataaatc cagctgaagc gaatcggtgt
      601 acacaagctt gctataggtt attccaacat gccttacttc ttgtccttca taatacttat
      661 caaacaagaa aagacaataa ttctgcaatt cctttgtatt atctgtgata gggatcttca
      721 tctggtgtga aaaccctggt ctcgtttcta agatcgacgt gccaatgtgt aaatggacac
      781 aacctgtttt acagttatgc tgcctaatgc gtatcgctac ttgttccgcc atttctttta
      841 ccaccagctc gatttcattt cttctagtat aatctcgtgg taacacttgt gaatttccat
      901 atgatttttc ctttgtcggt tctggtggga ttgctatatc tgttcgatcg atcccattag
      961 catggaagta cagctgtaat ccaatcacac ctaatctatt cttaatcgta taaggattcc
     1021 agttagcaag gtctctaatg ctcaaaattc ccatttggtt gagccttttt ttcattcgcg
     1081 aaccaattcc ccaaaagtcc gtcatttcgg gaatattcca tactttctca ggaacgtttt
     1141 cgtacgtcca ttccgcaata aatccttcat tatgctttgc ttcattgtct aaggctaatt
     1201 tagccaaaag gggattatcg ccgacgccta ccgtagcaat caaacctaac tcttctttaa
     1261 tacgttcttg gatcatttga gcgagcttct tccttcgctg acttcgtgtt tcttcagtcg
     1321 taaaaagatt cagtgatttt gtcactttta aaatcgattc atcgatcgag taaatcagta
     1381 gatcttcatc agccacatat cttctgaaaa tattatttac ctgcatattt cgcttgatat
     1441 ataggttcat acgtggtgga acaacgtgta gtgttttagg aaatggttgt ggtaagtcac
     1501 gtggtctact cacatttgta ataccatacc gcttttttgc ttcaggagaa gaagctaata
     1561 tcaatcctga accagtatta tcagatcgac tcataacaac aagttctgtt gttaatggat
     1621 ctaaatttct ttctatacat tcgacacttg cgtaaaagct tttcatatcg attaaaaagt
     1681 aatcatttac tggctctttt gaataatcca tcataaataa cactccattc ttccacatta
     1741 tacaaacata tgttcgatgt tgtaaagaag aaatagctaa atatttgaga ataagttatc
     1801 agtagacact tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat
     1861 tttcagcatt aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt
     1921 ttgccaaata acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa
     1981 taggtgtacc gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt
     2041 caggacgacc accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt
     2101 ccaggatcaa attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc
     2161 ctgtagaact tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac
     2221 gctcattcag tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta
     2281 agcgccaaac gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg
     2341 gtcgattact ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat
     2401 ttaggctgtc ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta
     2461 ttttcaaaaa aatcactcct tcattaattt tattgtaaca taactttatc catatagttt
     2521 aactgaacat agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa
     2581 taaagcaaaa aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa
     2641 aaaagaatgt cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt
     2701 tttggtctct tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa
     2761 ttttttgtgt ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat
     2821 taattttact tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca
     2881 taaaaaatcc ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag
     2941 ctgatttata actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt
     3001 tccaattccg tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc
     3061 ctgtcactcg tatcgtttta gaagctgctc gagcaagtaa tggagaaact attaaataat
     3121 tttcagtaac agttaagtcg ttagttgggt taccaatggc tattccgttc ctatctaaag
     3181 gagttaattc ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg
     3241 taggaattgt agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa
     3301 acttattttt cattgtctat cacctcgaaa tcaaaaataa gaaaacgttt tcttaataaa
     3361 tcttaacaga taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat
     3421 aatcgtttaa attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa
     3481 aagtgacaca tactatttcc tccatcatta gtttttgtct atataaggtt taccctaaat
     3541 agacactggt ataacattga aaaatatggc ttcctcgtgt atgattaggg tgtattttaa
     3601 atagttgcag aaagtaggta aaagttttga gagttgcata catacgtgtc tcatcgatcg
     3661 atcaaaatga acaacgccag attgaagaaa tgaaaaaatt cggtgcagag agaattttca
     3721 tcgagaaaca atctggcgca acgattactc atcgacctgt tttccaagaa gcattagatt
     3781 acgtccgaga tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattatg
     3841 acgagatcat cgcttcagtc aattacttga agaagaaaaa tgtgaagctg atcattacta
     3901 gtttaccgat catggctgaa gcaattggca atcctctcct ggataaattc attaaggatc
     3961 tgatcatcca aattttggcc atgatcgctg aacaagaacg gactgaatcg aaacgtcgcc
     4021 aggcacaagg aattaagatc gcaaaagcaa acggcgtcta caaagggcgg cccaaattat
     4081 acagtgcaga cgcaaaagat ccccaacgtc gccttgttta caaaagcatc gttgaagatc
     4141 ttaaaaatgg tgttgcgatt gctaagattg ctaaagacta caatgttacg agacaaaccg
     4201 tgtatagaat aaaaaaagac agcatggtca atgacaaata gttgatcatg ctgcttttat
     4261 tatcttctca tctttaattt cctacatata attactatct ataaaattaa gtcttactca
     4321 gaacagataa agcataaaat tccaacaaac cattttttac ttaactaaat aaagagtctg
     4381 ttaatataat cttcacttat ttacgttaat cccctactgt atttgttggt tccttatcta
     4441 tattttctac aaaatcctta ttatcttgtg atttagcttc gtataattct ttgtatgtcg
     4501 acacattggt tatactaatt cctatagcta gttcttgaga ctctactttt ctctctagct
     4561 cctttgacaa agtgatttga gatttatttg gttccgttgg agtcaattct atagattctt
     4621 cataaaaatc tctatacata aagctatcat taattttata aaaaaaatta aatgttttta
     4681 gcactgccat ataacattca aaacattctt ctatagttgg tttacttcct tgaggagaat
     4741 gaaccaattt attacgtttt tctatgaaat ttttaatttc ctttcttatt tctttcggaa
     4801 actcaatatt aaagtaccct ttaaacatgc tccctaataa tttgtctaca gggggagatt
     4861 gtaaattctc aaatattaga gttaaatcag gtttctcttg ttgataaaat tcctttaccc
     4921 ctatttcaca ggctgtaatt atcgaaataa acctatctct agtatttgat atatttttac
     4981 ttcttttaag gagtataaga gaaaagggga tactttcgtg tccatgcatg taattaacta
     5041 attgttgagc ttcagaagtt atatctctaa ttatatagtt aacggaatta ggaagtgtct
     5101 ttaactctaa tgaaatttct atccaattac tatcttctaa tctgtatatg ctcgaagtat
     5161 aatttgattc gtcagcaagc atcgctacat cccaaacact gatcataaaa tcttttaaac
     5221 gttcaatcat ttcagcaata ttgctaaaat cagctaattt ttctttagaa taaaaaaaaa
     5281 catttatatc agaatcaata aattctattt tttttattac tatatcgtca tcattatctt
     5341 gaccatttat gtttattgtt ccctgagtgg ggagtacttc tttcaaattc aagttaaatt
     5401 tatattccat tacttctcct ccaatatata aaaattactt aactgtatcc aaatttaata
     5461 tgtaaataaa tcattaattt ttagctaaat tctatgagaa gtttattttt gttgtttttg
     5521 ctaattaact aaaactagta caaaaaattt aaaatagatt attaaaccta ttgcttattc
     5581 tattcagtca tatatataag cgtcatcctt tagtaactaa catcccacac tacaatagta
     5641 ttataacata tctattcaac ttcaaattgt tgaaatttaa gccatttata tttacttttc
     5701 ctcaacttta gaaatgattt tactaacctt atatccgtaa tacgcgtaac aaacacattc
     5761 attaataaca atcaacacta agctatatta gctttttaaa tgactagggt aaataaaata
     5821 aggatcaaaa acttttaagt ttctgaccct ttccttactt ccgattggtt aatagtgatc
     5881 ttttgcagaa taaataaata tcgtttcatt ttcaactcta tatatcagtc tatgttcatc
     5941 tgtaattctt ctggaccatt ttccagataa atcatgcttt aatggctcag gttttcctaa
     6001 tccagcaaag ggggaacgat cgatatcttt tattaacttg ttaatctttt ttatattgct
     6061 tttgtttcct tgctcatgcc aataaagata atcatcccaa gcatcatcag accaagcctt
     6121 aatcatcaga ttcaacctcg attaagtcat gtgttttaaa tgcacctttg gagaattgtt
     6181 catctcctcg acgaattttt tccatgacgt aattattaga aagtgttctc aacgtttctt
     6241 gcatagaatc ataatctctt tttgataata caacaactgt atcttctaca tctttacttg
     6301 ttacaataag tgtttcagca tcctcattaa cttgtttcat ataactacgt aaattttggc
     6361 ggaaatttga ataagctact gcttccattc ctttcacccc ttatttattt ctataaaaca
     6421 attgtacatt atattgtaca attaagcaaa tgattaatat tctaacttct tataattaaa
     6481 agtgctatca cttttagttt ttaggataaa aagtgacgtc actttttgag tgctggtacc
     6541 tcaaatcctg ctttttcttg ggcaatcaat aaatctaacg caatacacat aatcctggat
     6601 tcacttttgc cgtacttctg ctttagttgc tgcaattctt tttgtctctt ttcatccaac
     6661 gtaacgggta accgtttatt tcctattgca attggcatta gtactctctc ctttcatcga
     6721 aataacccta tgaaaaatat ggctgcaatc atcttgaata gtagactcac aagggctaat
     6781 acaaagcgcc aaaagatcaa acaaaatacc agtattgcca aaaatactac gaagtttaaa
     6841 tgcacagctg cagcatggcg cataagtatt tcgccaacaa ataggactaa caatagagga
     6901 atgattttat tcatatggat ctccttcttt ctcttaggtt atcgggtgtt gcccgcttat
     6961 ttcgcaccac ctaagcggcg ggggcggttg gagcgattgg tgcgaaataa gaaccattcc
     7021 cttagttaat aggttttttg tccttatttc acacattctc acttttcggt ataaatgatt
     7081 attgcctttt tttcctttca aatcgttgat atggcattcg tttacggtca agttcatttt
     7141 ggatttcttc tgtatcgtcg taagtatagg tgtctaaaaa atcagttaaa gtcgtgtacc
     7201 agtcgtcgcc tgggttatct acttcttcta catattccct caaaaatgct tcgggtacag
     7261 cgagttcata atccattccc attaacacga tagattccgt tccacacgtc aaaaatctag
     7321 taaatacaag gtaataatcg tctatcgtcg catctttgct gtttagctct tccatttctt
     7381 cttccattaa ttccatgctc tcaaaaattg aataatattc tgattctgag taatagtgtt
     7441 tcctgcaaat cgtgtagctg acaaagtttt cgaccagctc actcctttct aggtaaacat
     7501 tcatgatgta tttttctgtt tctagtcgta gaagctccgt tccttttttt accgtttcag
     7561 gacgacgttt atacacgtct aaacgattat ggctgactgt gaccttcgtt tgactcgttt
     7621 cactttccaa atacaattgt tcttc
//
LOCUS       DACEOE010000119         7511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEOE010000119 DACEOE010000000
VERSION     DACEOE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.121"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(2..2167)
                     /locus_tag="IW774_002056"
     CDS             complement(2..2167)
                     /locus_tag="IW774_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1154393.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2427..4721
                     /locus_tag="IW774_002057"
     CDS             2427..4721
                     /locus_tag="IW774_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1154394.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFKLLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            4714..5379
                     /gene="pgmB"
                     /locus_tag="IW774_002058"
     CDS             4714..5379
                     /gene="pgmB"
                     /locus_tag="IW774_002058"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1154395.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFTKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            5372..6379
                     /locus_tag="IW774_002059"
     CDS             5372..6379
                     /locus_tag="IW774_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1154396.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            6401..7420
                     /locus_tag="IW774_002060"
     CDS             6401..7420
                     /locus_tag="IW774_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1154397.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         2342 a         1639 c         1409 g         2121 t
ORIGIN      
        1 cttacatttt aatggagccg atcactttac cagctgtcac tgtacctaat tgcttgatca
       61 ctacttcttc tactttatcg tttgtaaata cgatcatgat cgttggatct ttttcttcgt
      121 tttggatcac atcaagatcc atctcagcta atttcgttcc tgttcctact tcttggcctt
      181 ctttgacagc aatctcaaaa gctggctgat tcatttggac tgtatcgatt cccatatgga
      241 tcaaaacttc gataccatct ttcgtttcca gcccaattgc atgtttgcta gggaaaatac
      301 ttgtcacttt tccagcgatc ggcgcaacaa cttctcttga tgttggaata acagcaaagc
      361 cgtcacccat cattttttgt gagaataccg gatcttttac ttcagataaa ggaatcactt
      421 ctccagaagc tgcggaaact agttcttgtt ctatacctaa gtattgctta ctacctgctt
      481 gtccatttcc tgtagaaacc atttctggct ctggaatctc tgctcctgat tgcagcagat
      541 cttcgatatc tgatttcaag acatctgctt ttggtccata aactgcttgg actccattgt
      601 ctttgacgat caatcccatc gcccctgctc gtttccaggc ttcttcagtc cctacttttt
      661 ctcgatcatt tacacttacg cgtaaccgag tcatacaagc atcgacatct ttgatatttt
      721 cttttcctcc cagcaaatga atgatctgga ccacttgctg atctgctgtt cctgttccgc
      781 tgttagatac aacggtttct tcactgtcat tgtcgtaatt tccgttacgt cccggtgtag
      841 caaagttgaa ttttttgatc aagaaatttg ctaagaaata cgtaacaaca ccaaaagcga
      901 tcacacacaa cacgaagttg ataagatctc cacctagacc cgctttgata gctaacggtg
      961 tccgagtcag caattcgata ttcccaaatg aatggacccg caatggcaag atatctgcca
     1021 tcgcaaatgc agccccttgg atcactgaat aaatgacata taatggcaca gcagcaaaca
     1081 taaacatgaa ctccaatggt tctgttacac ctgttagaaa gacagccaac gctgctgaaa
     1141 aatacattga tttatatctt gctttcttat caggatcgac attacgatac atcgcaaaag
     1201 ccattcccat caagatacct gaagaaccaa tcatttgtcc tactttgaaa cgagccggcg
     1261 tccagttatg caacacaaac tcatattgcg acatatcccc tgcaccttta agattcacta
     1321 aatcagtggc ccaagccaac cataacggat cttgcccaaa tacttgtgtc cccgcctgtg
     1381 cgcctgataa aatctcatac gatccgccaa gctgtgtata gttgatagga atcgtcagca
     1441 tatgatgcaa tccaaatggc aatagtaaac gttctaatgt tccaaataaa aacggtgcta
     1501 gaatcggtgc gctttcttgt gattctgcaa tccataaacc aaaattattg attccagcct
     1561 gtacgtttgg ccacaccaca gcaagaatca atgccacgat tgttgaccat aagataacaa
     1621 caaacggaac aaagcgcttt ccattgaaaa atgacaaagc atccggcaat tttctataat
     1681 tatagtactt attataagcc attgccccga caaatccagc gatgatccct acgaatactc
     1741 ccatattcaa agctggtgct tccaatacac ttgtaaaaaa tcctttaacc atgatcttcg
     1801 taccgaacag agtgtgggta aatgcatctt cattcgcaag catgtcactt gtgacaccaa
     1861 agatagcccc agtgatccga ttgattaaaa taaatgagat cccagctgca aatgctccgc
     1921 ctgctctttc ttttgcccaa cttcctccta ttgctaaagc aaataaaata tgaagattac
     1981 caataattgc ccaaccaata ttttcaatta ctccgcccgt tgtgacaagc ggcgtccaat
     2041 caggattgat caagggaatt gatttcccta tactgatcat caacccagct gccggcataa
     2101 ctgctaccac aaccattaaa gcctttccaa atttttgcca aaactcaaaa ctaaatatct
     2161 ttttcattcc gtttagctcc tttacgcaat cggtttcgtt aaacgtatta tagcgtgcgt
     2221 tttctttttt gtaaatgctt tattttttat ttttctgtct ataccattct ataaaaccta
     2281 taatacgtgt gttcccttct ataattttct cctaaaaatt tattttttta agcttttttc
     2341 tatgaaatat tttcttttgt ctattgactt gtgcaatcgg ttgcgtttat tatgaacgta
     2401 taaaacagct cattaggagg agaaaaatga aacagatcaa acgactattt gctattgatc
     2461 catggaaaat cagcacgaca caattagata aaaaaaatct tcgactgcaa gaatctttaa
     2521 caagtattgg aaacggttat atggggatgc gcgggaactt tgaagaaact tactcagggg
     2581 accaccatca aggtacttat ttggcgggtg tctggtatcc cgataagaca cgtgtcggct
     2641 ggtggaaaaa tggttatcca gaatactttg gaaaagtgat caacgccttg aactttatcg
     2701 cgatagatat ttcgatcaac gatcagctga tcgacttggc ttctttggaa ccagaagact
     2761 tttactggga attagatatg aaaaatggtg tattatctcg tacattcaca atcactactt
     2821 ctactaataa agtacgcatt tccttcgaac gttttctgag cattgtcaaa aaagaatcag
     2881 cttatatcta tttaactata gaaatgcttg aaggtagcgg aacagtcaaa ctcgtatcaa
     2941 aattggatgg aaatgttcaa aacgaagaca gcaactatga tgaacatttt tggaatgaga
     3001 ttgatcgtgg aactatagaa gaaatcggct atttgacgac taaaactatt ccaaacaact
     3061 ttgggattga acaatttact gttactgcag caatgcgcca tttcgttaat ggaaaatcag
     3121 tcatccctga ctatcacgaa gatactttag cattacatgg ttgcttgtct gcagaattat
     3181 ctcaaggtga gacatggtca gtggaaaaag aaattatcgt tcttactagt agagatattc
     3241 cagaagcgca gcaagcagaa aaagctgtct cagaattaac gaaccagaca aacaattata
     3301 tccaagcaaa agaagagcaa acagcggctt gggcagaacg atggaagctt gccgatgtgg
     3361 tcatcgaagg agacgaagaa gcacaacaag gtattcgatt caatttgttc caactattct
     3421 ctacttacta cggcgaagat gaacgattga atatcggccc caaaggattc acaggagaaa
     3481 aatacggcgg agccacttac tgggatacag aagcgtatgc tgtccctctt tatcttgctt
     3541 tagccaaacc tgaagtaact aagaatctgc tgaaatatcg gcataatcaa ctgccgcaag
     3601 caatccacaa cgctcagcaa caaggattaa aaggcgcgct ttatcctatg gtcaccttca
     3661 ctggtgtcga atgccataac gagtgggaaa tcacattcga ggagattcac cgaaatggcg
     3721 cgattgccta tgcaatctac aattatacga attacaccgg cgatacggaa tatttgaaaa
     3781 atgaaggact ggaagtttta gcagaaatcg cacgcttctg ggcagaccgt gtccattttt
     3841 caaaacgagc aaataaatac atgatccacg gtgtgaccgg cccaaacgaa tatgagaaca
     3901 atatcaataa caattggtat acaaatacga tcgcagcttg ggtcttgcgt tacacgcgag
     3961 aaaattattt aacttatcaa aaagaagctg ctatcactat cagcgaagaa gaactagcca
     4021 aatggaaaga tatcgcagag aacatgtact tcccagttga cgaagaatta ggaatctttg
     4081 tccagcatga cacattctta gataaagatc tcatgcctgt atccgatatt cccacaagcg
     4141 atcttcctct gaatcagcat tggtcttggg acaaaatcct tagatcttgc ttcatcaagc
     4201 aggcagatgt actacagggg atttatttct tcaatgatac ttttacaaaa gaagaaaagc
     4261 aaaaaaactt tgatttctat gaaccaatga ctgtccatga gtcttccctt tcgccaagta
     4321 tccattcgat tttagcagca gaattaggaa tggaaagcaa agctgtagaa atgtaccagc
     4381 gaacagctcg cttggatttg gataattaca ataacgacac agaagacggc ttgcacatca
     4441 cttccatgac tggtagctgg ttgtcgattg tccaaggatt tgctcaaatg aaaactgccc
     4501 atgatcagtt gagattcgca ccatttctgc caaaaacttg gaaagcttat tctttccata
     4561 tcaattatcg cggacgttta ttgttcatct cagtcacaga tgaacaagtt actttcaagt
     4621 tattagaagg ttctcctcta gaaatttacg tctatgaaca gtcttattct ttagcggatc
     4681 gtctagaaat aacgctaaga aaggaggaag caaatgttta aaggggtatt gtttgattta
     4741 gacggtgtca ttaccgatac tgctgaattc cattatcgtg cttggcgaaa gcttggtcag
     4801 gaaatcggca ttacgatcga ccacacattc aacgaacagc taaaaggcgt cagccgagaa
     4861 gactcactct ctttgctttt agcttatggc ggaaaagaac actcttttac aaaggaagaa
     4921 tttgcagaat tagcgaaaag aaaaaacgat tattatttag agatgatcca gacgatcgaa
     4981 ccaaaagatg tcttccccgg ggtcgtgcct ttactggatt ctttaaaaga agaaaaaatc
     5041 aagattgctc ttgcttctgc aagtaaaaac gggccattcc tacttgaaaa aatgggtctt
     5101 acaccttact ttgatgctat tgccgacccg gcagaagcaa caaacggaaa acctgcgcca
     5161 gatatcttcc tccttgcagc aaaagctgtc ggactaaaag cagaagaatg tatcgggatc
     5221 gaagacgcgc aagccggtat ccaagccatc ctctcaagtg gcgctcaacc aattggcgtg
     5281 ggacgtgctg aagaccttgg agaggagatc ccgcttgtcc ctgatacgac ttttcttacg
     5341 atcgattact tgaaagaaaa gtggcatgac catggataat ccagagatct ccgtcgtacc
     5401 agttccagag actgaaataa agaaaatcac attgaagaac gactacttag aagtcgttct
     5461 tctcaattac ggtgcccgcc tgcaccaaat cttcgcacca gacaaagaag gaaaatcaga
     5521 aaatgtttta ttgtcttacg atcgtttcga agatgttcta acagacaaaa gcttttttgg
     5581 agcaactgtc ggacctgtag ccggaaggat ccgggatgct tcttggggtg agcatagatt
     5641 ggagaaaaac tgcggatcac accatatcca cggtggcacg aaaggctggt ctttccaatt
     5701 ttgggaagta gaagttttca aaactccatc atcaataggt gtggtctttt acttaaaaga
     5761 tgaattttct acctatccag gaccaatcac tgctacgatc acttaccgtt taacaaaaaa
     5821 cgaattagag atgacaacct ctgctagtag ccttgttgaa accatctgca atccgaccaa
     5881 ccatgcctac ttcaaccttt caggaaatgg aacaagagac atctatgaac atcagttaac
     5941 ggtcttttta gatggtgtgc ttgaattaga tcaagaaaaa ctgcctactg gaaattggac
     6001 aaaaaaagaa aaactgccaa tcgactttag aaaatcccca acactgcaag agatccttgc
     6061 atgttatcca gatggactag atgatgtatt tcttttgcat catcctaggc tttccaaaac
     6121 ttccttacaa ttattcgaga agcattctgg tcgccaaatg actatagcaa cttctaataa
     6181 aagtatggta ttattttcta cgactggatt cgaagctgat ttctctgtca atggaaaaca
     6241 gatgcattca aactacggat tagcaatcga accgcaagaa atccctgata tcgttcattt
     6301 ccccaaattt ggatcgatca acctccatcc tggtcaagaa cggatcagtc agacgattta
     6361 tcggttctct gcacaataac aagagaaagg tgtttttatc atggctatta ccgtaaaaga
     6421 tgtggcgaaa aaagccggtg ttgccacctc gaccgtctct cgtgtcatca acgatcatcc
     6481 tagtatttcc gagacaacaa agaagaaagt ccgaaaaatt atggatgaac taggttatgt
     6541 tcctaacatg actgcccgca atctaggaaa acgcatttcc agtgccatcg gcgtaatctt
     6601 accccctctt gattcaaaag agcggatcgg taacccattt tatcttgaga tcatggaagc
     6661 aatcaacgaa gaagcgcgga attatgatat gactacagca atcgctactg ctaaaaattt
     6721 tgaattgctg ttagagaacg tccagcgtat gcatttacaa aaacaagtag atggttttat
     6781 tctagtgtat tcagatagtc aagatactgt cattgattat ttataccaaa accacatccc
     6841 tttcactttg atcggacagc cttatcatca tgagaacgaa atcgtttatg tagataatga
     6901 taaccagctt ctaggtaaac aagcaacaga atttttgatc cagaatggac ataagaatat
     6961 cctgtttgtg acaaacacga ctcatgaaag tttgtatttc gaacgatatt tcggttatca
     7021 aaaagcaatg atgatggctg gtcttcctgc gttatccagt gtagtactag agcatccgga
     7081 ggattatctg tcctttgaga aaacgttgaa agaaaacgaa gccacagcat tggtggtcat
     7141 tgacgatatc ttcgcccttc gcacgatgca gttggcgcaa atgtatggct atgtcattcc
     7201 agagacactc tccgtcatta gcttcaacaa ctcgatcttc tcaacattaa cacaccctta
     7261 ccttacaagt atcgatatcg atatctccga attaggacgt gtcgctatgc aaaagctgcg
     7321 cgaattgatc catgaagaat tgagcagtgg cgtacgtttg gtcatccctc atcgcttgat
     7381 caaaagagag acagtcgttc ctttagaaaa cccatactaa atagctgact cactaaatac
     7441 gctgaccatt tatcctatgg tcagcttttt atgtaaaaga agatactact tctaacacat
     7501 tgtttatctg t
//
LOCUS       DACEOE010000120         7483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEOE010000120 DACEOE010000000
VERSION     DACEOE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.122"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..587)
                     /locus_tag="IW774_002061"
     CDS             complement(<1..587)
                     /locus_tag="IW774_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1154398.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQ"
     gene            complement(714..1187)
                     /gene="dfrF"
                     /locus_tag="IW774_002062"
     CDS             complement(714..1187)
                     /gene="dfrF"
                     /locus_tag="IW774_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1154399.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(1606..1824)
                     /locus_tag="IW774_002063"
                     /pseudo
     CDS             complement(1606..1824)
                     /locus_tag="IW774_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1855..3069)
                     /locus_tag="IW774_002064"
     CDS             complement(1855..3069)
                     /locus_tag="IW774_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1154400.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(3410..3652)
                     /locus_tag="IW774_002065"
     CDS             complement(3410..3652)
                     /locus_tag="IW774_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1154401.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(3684..4241)
                     /locus_tag="IW774_002066"
     CDS             complement(3684..4241)
                     /locus_tag="IW774_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1154402.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(4254..4442)
                     /locus_tag="IW774_002067"
     CDS             complement(4254..4442)
                     /locus_tag="IW774_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1154403.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(4455..5021)
                     /gene="amaP"
                     /locus_tag="IW774_002068"
     CDS             complement(4455..5021)
                     /gene="amaP"
                     /locus_tag="IW774_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1154404.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            6251..6724
                     /locus_tag="IW774_002069"
     CDS             6251..6724
                     /locus_tag="IW774_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1154405.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            6733..6960
                     /locus_tag="IW774_002070"
     CDS             6733..6960
                     /locus_tag="IW774_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1154406.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2293 a         1453 c         1111 g         2626 t
ORIGIN      
        1 atttgaatta gtccacaaat atctttatta caccaataat caatcagcca aattgctttt
       61 tcattgtcgt ttactacatt caacgattct aagtatcctt ttctttcttt cggtattttt
      121 cttttcaatt catcataaga taaccgttca agcattttct cagtgctttc ccatacttcg
      181 aaaatatata aatataattc ttccagatta agctgttttg aaaagtctgc aatctgctgt
      241 ttcatgagtt catttcctgt tgtaatgata ggtgaattga tacgctcttg ataattgcct
      301 gcaaaaaata cttgttcatc ttcatttatc actgtatgca caactatatc ttcaatgcgg
      361 aaaatatgcc aaatagaata agcaatcgtc ttactatgat aaccatcagc gtttatgaag
      421 gggattgagt taaaatcttc tctgcataat tcttctttga aagatactaa ggtttgtatt
      481 aactgacttc gtaaagtaag taaagtatca atacccctct tataagtatc tttctttttt
      541 atttgtgcct gcattgtttt attaagttca gaccattcct tattcatatt caatcctctc
      601 tcaaattctg attttatcgc cccatacaaa ctggaatttg tcttacaaca atttatatat
      661 gcataaaaag caatatattt atgaaaacac cctaaatcca aaatttactc aattcatttt
      721 ttccttacat aaaatgttct cgtatatttc acttcctcac caagtgtttc ccctatcaaa
      781 acttcaaaat cattgatatc aaattctgga aagaatgtat ctccatcttc aatatttaaa
      841 tctacttctg tgatatacat tttatctact atttgcaaag cttccttaaa tagtccatat
      901 ccaccagata tgtatacatc tcgtccttta gccaataata atgcatcttc taatgattta
      961 actgaaacta aattatctcc ttgatactct gttgtggtgg aaacaacaat attcattcta
     1021 ttaggcaacg gatgaccgat ttcttcataa gactttcgcc ccataataac cacattaccc
     1081 gttgttaact ctctaaattg cttttgttct ccctttattt tccatggtat attaccattc
     1141 ttgcctataa cattattctt tgacctcgca acaatcaaac ctatcatcta tttcatcctc
     1201 cataatatga tgtcgtacct gtgattccat caagtgcacc acatcttaac aaactcaaaa
     1261 ttatctcgaa ctaaccagct cgacaaattc cttttttgtc ttcatctgca ttatatcata
     1321 ttgttataag cattgccatt tcttttctgc ttttgaaaag ttacttatca gcctatgttt
     1381 tgtaaagggt gatattcagc atttctcacg caacaagccc ttgacaaacc atagactaat
     1441 aagttttaag gtaatcaagc agaaatgaaa tagtctgctg tttcacggca acaaaaaaga
     1501 gataccaaca cacttatgta gtatctctta gtctagtagc aatattcttt tgtttgcaag
     1561 caggtaaaca tgggctgttt cttaatatct ttttgtttgg gaaacacctt tttcccatcc
     1621 tttcaccgtt gccatctctg ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc
     1681 taataacaag aaaaatacta agctgaagcc tgaggtcgcg aatgtgggat attcatataa
     1741 tgaaacgacc attgagatgg tcatagccaa aaagccaaag aacgttaatt tttttgcagg
     1801 aattttcgtt ttgctttgtt gcatgaatct tcctcttttc tatttgaatt tgtttcaatt
     1861 gagtttgttg ccaaagaaga aaaagtccaa tccttcttct tgttcttctt ttgtagtagt
     1921 gatcgttgta aagtgcatcg ccgattttaa aatcactgtt gtcagcacat aggcatacac
     1981 ggcagtaatg atgacaccaa tcacttgaat ccctaagaag taccagctgc ttggattcgt
     2041 tagtaaatgg ctgttagatg caaagatccc tatcagaatc gtacctgtga atccgcccat
     2101 gccatgaaca ccccacacat caagtgcatc atcccattgt ttctttttac gaaaatcaac
     2161 agcagcatga cagacgatac ccgcaatgat cccaatcagc atggcacttt ttgcttctac
     2221 ataaccggca caaggcgtaa tggctgccaa tccagcaacg gatcctgtta gtacgtcgac
     2281 aaaatcaaag ccatttcctt ttacttttgc aatgatcagc caagtaatca tcgcaaatgc
     2341 aagagcgatg aatgttgaac caaatgcatt gactgcttgt tcatctgctc gaagtgcacc
     2401 accggaatta aaaccgaacc agccaaacca aagcagtcct gtaccgatag cagctgccat
     2461 aagactgcta tgctgacttc tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat
     2521 acatgctaaa gagccgaatc cagctgtcgt gtggatgact gctccaccag cgaaatctct
     2581 aaatcccatc tgatccaaaa atccgccacc ccatatccag tggcaaacag ggaaataaat
     2641 cagcagattc caaaaaataa caagtataat atagcctttt aaattcaatc tccctgcaaa
     2701 agctcctgtc atcaaaggaa cggtaatcac gataaacatc agttggtaaa gaaagaacat
     2761 taaaaatgga atcgttgcac catgaaacat gttggggaag cttgtaacat gtctcatcaa
     2821 aaagaaatct gccgggtttc caataacacc gccgatatcc cgtccaaagg ccaatccgaa
     2881 tccaccaaat acccacataa tcgttgttat cccaacggag agaaaagatt gagacatgat
     2941 cgttagtata ttattcttcc gtgctaatcc gccgtaaaaa aaagctagac caggagtcat
     3001 caggcaaacc attgctgtac ataaaatcat gaatgctgta tctcctgtat tgataggtaa
     3061 tgtattcatt tttttcgcct cctttctacg attacgagta taaaagaagg tgtagttgtt
     3121 tttttcttct gtttttttga attaatactt gcatttataa aagacagaca attaataaaa
     3181 gcttgatgtg ttgtgctctt tttcgttttt cacacaaata aagacaaaaa ataaacggtc
     3241 ttctttttat agaaaaccgt ttattttcgt ctttttttat ttttaaagat attttttcat
     3301 aaggaataaa gaatacttca tataaaatat ataaaaaaga accgcagcga gtgtcgctac
     3361 ggttctcaaa tttgttcttc atttcaacat aagtttttct tcaatttgct tataatttac
     3421 gtgcaaagaa tgaaacgact aatactaaaa tgattgcacc gatcaatgat ggaatgatag
     3481 ccattcctgc aattgttggt ccccaagttc ctagtaaagc ttgtccaatt gctgaaccta
     3541 ctaaaccagc aatgatgttt gcgatgatcc ccattgatga tcctttattc gtgattgctc
     3601 ctgcaattgc tccaatgata ccgccgacga ttaatgccca aataaatccc attttaaatt
     3661 cctcctaact gacttcttac ttcttattgt acacgtggtt cgttattttc tttgactttt
     3721 tgtgcacctt tgcctacagc agattttgct ttatctgttt ggcgtgaagc aaattgtcct
     3781 gtggattttg ctgcttctgt tactttgtct tgtactgttt cttgatcttt ttggtattct
     3841 tcttgtgttt tgatgtctgc aacattcaca ttcacttcga taacatctaa atgtgtcata
     3901 ttgtttactt cagtactgat caattctttg atttggttgt aaatatcttc gatgtcttta
     3961 ccgtattcta cgatcacatc tagatccaca gctacttgtt tcttacctac ttctgtattg
     4021 atacctgctg ttacgttatc tgtgttgact aatttttcag ccatatttga gaaaaatccg
     4081 ccatctaatg tcaatagacc atctacatct ttcaatgcat aaccgatgat tttttggatg
     4141 actttgtctt cataagtcaa ttcaccattc atcatgcggt tttctccttt ttcatttgcc
     4201 aaaactgatc catttgttgt attcatgttt ttgttttcca tctttgtttc ctcctatgat
     4261 ttaaaaaatt gatcaactat tcctgactgt ttcaaataaa aaccgattcc tgcgccaatt
     4321 gctgtcatga ttatgatcaa caatgtcttg aaaaatccaa tcgttaacca taaaacagct
     4381 agcagtagtc caagaccccc aaagataata gggagcttgt atgctgaaaa tagttctttc
     4441 attatttggc cctcctattc cactcgagag tgcttgtagt ctcttgtatc ttcttgttct
     4501 tcataaccgc tgtatgtgac tgccactttg accgtttctt ttgttccgag tactttagtt
     4561 acttcttctt cgatatcttg catcaataca cctgttttcc ctactaatga agaagttcgt
     4621 ttcaagtctc cgttgacatg gatatggatt cgatttttcg ttgaacgtat ccgtacttta
     4681 ggtgaatgaa tgaactcttc ttcgtgtaaa tgggaacgta ccaacccttc gatcgctttt
     4741 ttgtcgattg ttagtttgcc atcttcccgt ttcataacga aagttcctct tgactttgga
     4801 tagaaaagaa cgactagcaa gatgataata gcaagaacgg caaatactac agctgcccag
     4861 aaaatatatt gttgcatgta gtaattcgtt aaagtaaaga agcgaaatcg ttccaattgt
     4921 tcaggcataa tatacggcgc attgatcaac gcaatcgcta gaaatactga aagtagcagt
     4981 aaactcatga taacaccgat tatttttaac cccttattca ttttccacct cctataaact
     5041 tatttttacc ctgtaaataa ctctaccgac aatcacagta tagatagcga acagttagca
     5101 tagcatcagt aaataaagcg ctaccaaaca ttgctggatc ataacggcgt tttattgcca
     5161 gtctgatact gaaataacaa ttgcttactt attctctatt tacttttgat tgctggctat
     5221 acttaaagga tattattcaa gattaaaaat tgcaaagatt atgctcagtt attattacaa
     5281 gattctattt atttattaaa ttttttcgga gaattaaaaa atacaataaa taactttagt
     5341 tactttaatg cgttcttact ttatttttat attatcattt ttcttattca cttctttggt
     5401 ttatactatt cttataaata aaaacgaggt atgtcctatg atgaaaagat tgtactattc
     5461 attgatcata acgattggct atttgattgt atcaaatcta ggaaatatgg tatttggtat
     5521 cagtaaagaa ttcagttgga ctactacatt atgggaatct ctattctttt tcatcttcgt
     5581 atttttgtta caaaattatc gaaaaaaata aacatgtgcc atctatctgt tggcacatgt
     5641 tttaggattt atggtacttt ttattcggca atccatttta tttttctact tcttcttaat
     5701 acctctaaca cttttgattt ataaactaat ccaatgcatc tacttgcgct tattaagcta
     5761 tttcagctag atagtaccac cccatgcgtc aatgcttgat tttccttttg tataacattg
     5821 tataagcaat aaaggacaaa taagtggtga ttacaccaaa ccattgaata agaaaaattc
     5881 tgatacgtaa ataaacatga acttgaaaaa attaatgaat cggttagcta ctacgtagtt
     5941 gtcttttttc tcatacagtt ctgttaactt acacttctct tcgctagata ataaaaaatc
     6001 ctccaactgt attgtccaat ataatcccag gcatctaaac tagcattata gaagagacac
     6061 acatagaatc taaaataact ggttttaaat gtttcttaca atcaaaaaaa gctgttaaat
     6121 gcactccaca ccttcatact ctatcatttt ttataaacga tatattttta ttgtttttcg
     6181 ataaaaaata gttgttttac aataattatt ctgctatatt ttagctatca aagataaaag
     6241 agggataaaa atgaaattta aaacattgct attaaaattt gtcgttttag gtataagtgc
     6301 cgtatttatt ttgttcggcg tactttttta tactcagatc gctacaagcg aaaaaggatt
     6361 caattttgat tggcagacca cattatttgt ttctgtcatt tactttgccc ttctcctagg
     6421 gatcgttatt gcttatttct tattgaaact attgaaaaaa atcgaagata accaagcttt
     6481 ttccactcaa agcttaaaca tactaaaaaa aatcgaacga accattttgt ttattggttt
     6541 cgcctttttt ggcttacttc ctaaatttat gaaagtagcg gatttagacg atgctccagg
     6601 tttgatgttg gtaggaatcg ctgtcgtatt cattcctttt gcagtataca cattaacaag
     6661 cgtgctggaa aaactgcttg aacatgcgat tttacttaag acagatcatg atttgacagt
     6721 gtgaggtgcg acatggcaat tatcgttaat ttagatgtag tattagctca aaaaaaaatg
     6781 agtgtgactg aattgagtga gaaagtgggc attacgatgg caaacatctc tattctaaaa
     6841 aatggaaaag caaaagccat ccgtttttcc actctcgaaa aaatctgcca agttttggag
     6901 tgtcaaccag gtgatttgtt gacctataaa aatgactcga atactatacc agaagaataa
     6961 aataaagtag aacaaacagt aaataaaaaa taatctgaat gatagatgtg tgagactttt
     7021 agaagttatt tacgtctatt attcagattt tttatttcga ttgaataccg ttttatctat
     7081 aaataagata tgtactgact catacaaagg gggccttttc ttctccatgt actatcactt
     7141 ttcctgatac aactggtttc ctgttacgtt gtagttggat ctgcttttag tataatactt
     7201 tattcattcc accagactga tagcagtttg agcagtctaa ataatcatgt agtattacag
     7261 gccattctgt ttcatacctt gaagtgctta aaacttcagc taatggccca ttgtgttaac
     7321 tcttactcca ttctttttta gtaagctccc atatatactg taaagccttc tatatattat
     7381 gtcatgttgc tttttaacat ttactatgta gttttgataa aatgatttga ataacagtga
     7441 tggacaattc ttttttattt ttaacattat tttgataata atg
//
LOCUS       DACEOE010000121         7431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEOE010000121 DACEOE010000000
VERSION     DACEOE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.123"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(449..1795)
                     /gene="gor"
                     /locus_tag="IW774_002071"
     CDS             complement(449..1795)
                     /gene="gor"
                     /locus_tag="IW774_002071"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1154407.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1929..3170)
                     /locus_tag="IW774_002072"
     CDS             complement(1929..3170)
                     /locus_tag="IW774_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1154408.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3188..4111)
                     /locus_tag="IW774_002073"
     CDS             complement(3188..4111)
                     /locus_tag="IW774_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1154409.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4332..6824)
                     /locus_tag="IW774_002074"
     CDS             complement(4332..6824)
                     /locus_tag="IW774_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1154410.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6831..7298)
                     /locus_tag="IW774_002075"
     CDS             complement(6831..7298)
                     /locus_tag="IW774_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1154411.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2041 a         1622 c         1188 g         2580 t
ORIGIN      
        1 atttgttcgg aaaaagtgac taactatgac acaatttttt tcacagtccc ttttttcact
       61 tgatttgatt cgtaccagcg aacttgacga gaactttctt ggtttggtaa ctatatggta
      121 ttcttttaac atcctcgttt gcttcttcta atttatttat acaaagtcac acttttttaa
      181 acatctgttc ttcgatacat ttctatttct tttaaacatc gataccaata ttttgaactt
      241 aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata atcaaaatct
      301 ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat aaataactaa
      361 aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt ttgttcaata
      421 aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct tccgctcctg
      481 ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc ttgatagcca
      541 cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag ccgacgattc
      601 tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa tcatttaatg
      661 caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg ccatattttt
      721 ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta aagacgactg
      781 tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa cgttctgata
      841 aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc acatcaccaa
      901 cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata aacccttttt
      961 tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt acacgtccac
     1021 cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc accgtatatt
     1081 cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt attccagctt
     1141 cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta agtggtcgtt
     1201 cttttcgata tgcccacatg acttcactac ctaaaccacg aaatacacct gaaagttcag
     1261 ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt aattcaaaaa
     1321 atccatttga atcaattgcg tattctccac caggaatggt cattttactt gcacggcctc
     1381 cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg atcgtttgac
     1441 tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct aatcctcttt
     1501 gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga ccggcgaaat
     1561 caacattttt taaatctgct tggataccgt aacttgaggc atctcgttca attgtttcca
     1621 aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg acacatgttc
     1681 cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct gctctgtttg
     1741 ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt tgcattttgt
     1801 tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt ctacagaaaa
     1861 caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg gatatatcag
     1921 ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg tgcgactggc
     1981 atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc ttcattatgc
     2041 ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc gatttcatct
     2101 cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc atcataagat
     2161 aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa taattgttcg
     2221 ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc tactttccat
     2281 aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat ataactgtct
     2341 acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt cattcttcct
     2401 tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc gatcattgac
     2461 atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt taaactgttt
     2521 cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga ttgagaacat
     2581 ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg accagaataa
     2641 gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt tttacgaatc
     2701 tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa cccttcttct
     2761 ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa atggatcggc
     2821 agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac aatcactgca
     2881 gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc atttccttca
     2941 tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc aaaagcaact
     3001 ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc atatgcattc
     3061 ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt ttccagtgtt
     3121 cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat ttctttcatc
     3181 atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct aattcacggt
     3241 tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct tgatcccagc
     3301 taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt acgaattttt
     3361 cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga taaagttcat
     3421 tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca tgactatctt
     3481 cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga cctctttcac
     3541 gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt ggacgtttgg
     3601 attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg agtttttctt
     3661 ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca actgcgcctt
     3721 ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca tcataaatat
     3781 acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta tcaccaactg
     3841 tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca tttttcattt
     3901 tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac ttgtgtgcta
     3961 attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc aatccaaatt
     4021 gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct gcttgttcaa
     4081 tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac ttcataatag
     4141 tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt tttgaaaatt
     4201 atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa aagagattgt
     4261 gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat tcagctacac
     4321 aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac attcaatgtg
     4381 atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc tccgccaagc
     4441 aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat tggacgtgca
     4501 ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg tgtgattttc
     4561 acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat ttggacgatt
     4621 gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat tcgattcaag
     4681 aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat ggctttgtga
     4741 tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat ttgtgtcgct
     4801 ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct acctttagaa
     4861 tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc tggatgtgct
     4921 ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt ttctgtcaac
     4981 tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg gcttgtactg
     5041 tatttttcca taaattctga catatcaaca cggatcaggg catcttcact tccaaacatg
     5101 acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc aaggaacatg
     5161 aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg gatcgcacgt
     5221 gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag tccttccagt
     5281 tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc agtccactcc
     5341 gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg ctcctgtttt
     5401 gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg tctgcgtaat
     5461 tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg tactagttca
     5521 tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc caaacggact
     5581 ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg agaagtgata
     5641 taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc cactccatgg
     5701 tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc aggagttggc
     5761 tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc gatatatttt
     5821 tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc taacgccggt
     5881 ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat taaggtatgc
     5941 aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat gacctttttc
     6001 attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc catatcaagc
     6061 atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat tttttgagct
     6121 aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac agggttattc
     6181 tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg accaacaact
     6241 gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga atctagcgta
     6301 ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg acgattcatt
     6361 ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg acgggctttt
     6421 gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc atcttttaat
     6481 aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt cacttcgctg
     6541 ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac tcctttaggg
     6601 taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat ttccgtttca
     6661 ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac cactaaggct
     6721 agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc ttcttgtgca
     6781 atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc tcacttccca
     6841 tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt atgatcttca
     6901 tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt tccttctttt
     6961 tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt tttctcactg
     7021 attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc tgaaatcctt
     7081 acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag ataaccacgt
     7141 tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca gttaaagagg
     7201 tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat tttctttata
     7261 tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc ctttgactaa
     7321 tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct ttttgttaaa
     7381 taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat c
//
LOCUS       DACEOE010000122         7399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEOE010000122 DACEOE010000000
VERSION     DACEOE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.124"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..380)
                     /locus_tag="IW774_002076"
     CDS             complement(<1..380)
                     /locus_tag="IW774_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154412.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVL"
     gene            complement(588..1514)
                     /gene="glsA"
                     /locus_tag="IW774_002077"
     CDS             complement(588..1514)
                     /gene="glsA"
                     /locus_tag="IW774_002077"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1154413.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1537..2748)
                     /locus_tag="IW774_002078"
                     /pseudo
     CDS             complement(1537..2748)
                     /locus_tag="IW774_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2783..4396)
                     /locus_tag="IW774_002079"
     CDS             complement(2783..4396)
                     /locus_tag="IW774_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1154414.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(4508..4717)
                     /locus_tag="IW774_002080"
     CDS             complement(4508..4717)
                     /locus_tag="IW774_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1154415.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            4808..4996
                     /locus_tag="IW774_002081"
     CDS             4808..4996
                     /locus_tag="IW774_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154416.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            complement(5706..6920)
                     /locus_tag="IW774_002082"
     CDS             complement(5706..6920)
                     /locus_tag="IW774_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1154417.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(7048..>7399)
                     /locus_tag="IW774_002083"
     CDS             complement(7048..>7399)
                     /locus_tag="IW774_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1154418.1"
                     /translation="SSLKKAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFL
                     YKEIPSYKYLVVEHIGAMDKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWN
                     SDNSVIEIWIPIQD"
BASE COUNT         2110 a         1576 c         1206 g         2507 t
ORIGIN      
        1 tccaatacga tttttttgat tgtatatact ttactcaaag aaataaattc aagatcagcc
       61 agttctgtac tcgtgaatat accattaaaa taataactta aaaactttaa aaaataagat
      121 tgaccgtaca gaaactgtgt gagctcagaa agagcaatac catgtttgaa tacgagttta
      181 tacttatgct tctctcttac ttgttctttt atacaatatt ctagacactc ggctagactt
      241 tctagatcgt gaaggatcgt tggtgtagtc acccttagag aaatagctgt ttgttcgatg
      301 tctatttctt ggaattcaaa taattgttca cacagataca cttttctaaa tatatttcgc
      361 tcgatgtaat tttccagcat tcctgataca cgcctccagt ttttcttatg attttattta
      421 atcatatctg taaatcgtat ttttgtttcc tttttattta gaaaagccct ttcaaaaacc
      481 aataaaaaaa gaaactggga caaaagtcaa acaagctcac aatttcagcc tcattgtagg
      541 tagctttgag ccgtttggac ttttacccca gcctctttca agaatggtta ttcaaagatg
      601 tcagcgtcta gttctttgac tacatctttc aacaattgga taccagctgc gctgttacca
      661 aatggatcta gtgccgggct gaagacacct atgccgtaac gatttggcac agctgccatc
      721 aacccgccgc ctacaccgct ttttgcaggt acaccaacat ggacggaaaa ctctcctgat
      781 tcatcataca aaccagcagt tgtcataatt gatttgacga ttgtagcact ttcttcagta
      841 atgatttgtt tgccattcca aggagcaatt cctttatttg ccagcacagc agctaatttt
      901 gctatgcctg tagcggtgac attgagggag cattgtctga aataagcatc gagtatctcc
      961 ggtacgtctc cttcgatcat ttgattacct ttcatatagt aagcaagaga gcgattgata
     1021 tcacctgttc ttgattcaga atgatagatt tcttcattca atgtgatatt aggatcatca
     1081 cagatctctt tcataaatga caagattcgc tcaaattttt catctgcatc tttaccggca
     1141 atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag gattcattgg atgtttgcga
     1201 tgattgattt ccatattcat gatagaattg aaggcaaaac cggttggttc cgtgttgatt
     1261 gtttgaaaga ctttttctat tccgttgtct ttgattgcta ataacaatac gggaactttt
     1321 gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta
     1381 tccaaatcat aaatcgccat acctaattgt ttgggatcca cttcagctaa tgctggaatg
     1441 taatccgcca cttgtcctgt ttgataatag tggaaatttt ttccaatcaa ttgttctaat
     1501 tcttttactt ccatttgaat aaccagcttt cattttttat ttttctgcat gctggaattt
     1561 atgacggcat gcgtagatcg taaatggaat aactaagacg ataagataag aaacaatcag
     1621 aatcgtttca taaatcccat gctgtccagc tgcgatacta gcaggtggaa caaatgagat
     1681 acaaaaagca aaaagtgaag tcaaaagacc taaacaagca acgattactt ttacaacttt
     1741 tccgcctgga acctgatagg ctctttcaag actgtcatct ttaaaaacca gcttcaaata
     1801 gcccgcgaaa aacataaagt acaacatcag gtagattaca acagtcaatg acatcgctgc
     1861 aagaaatgaa agattattcc ccccgccgct taatgtaaag acggctgccc agattgttgc
     1921 aacgatccca tttgcgatca tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa
     1981 atattcagga agtaatcctt cttctgctgc tacgtgaagt gcttttgaag gagaggttac
     2041 ccaagaagca atttctccga tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat
     2101 ccatgcaaga tttggatcaa tgtgtcttaa taggaattct aacgattgaa tcacccctgt
     2161 attaagagac aaaccatgtt gaggtactgt agtagcgacg gtcaatccgc caaaagtatc
     2221 taaaatgatt gccagaataa ccaatacaaa cattgctaat ggataatcct ttttagggtt
     2281 ttccatttca tttgcatgac tggcagatgt ttctacgccc atatatgcta aaataaagga
     2341 aacaaaaaca accagcgtag gcaatttcga aaaatcagga atcaatgttt tcatagacag
     2401 tatcgtttgt agtggagcgc ctgaagcgac ataatggatc cctaagccaa acaagactaa
     2461 tgctggcaac aagataccag ctacaaaacc gatccgagca atcaatgaag tgtatttggt
     2521 tccttttaac tgtgagagta aaatagccca gaaaataatc aataggattg ccaatttcag
     2581 atatttattg ttttgtatag caccaatacc cgttgcatag gataatgcac cagttaagaa
     2641 atacagcatg gtcaagtagc caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa
     2701 accgaatcgt tttcccaatg tgcgactgac ccaagtatag acaccgccgg tttcccttag
     2761 ttaaaaatat tactcttgtt gattactcaa ctcttccaat aaatcggtaa tatatctcaa
     2821 tttcctgtat tctttcgtta tcctcattcg ttgttgcttc atgtatgagg attttttcta
     2881 tcatggcatt taataatggt gctgtcagtt cctttggtac ggaatattcc ttgattaact
     2941 caatccactt ttcagcacct atcatatcct gttccatttt acttagttct tctcttaggc
     3001 ttgttatctg ctgttccagt tctatctgtt ctttttggta cttgctggac aacatcacaa
     3061 aatttcgctc tgttatcttc tcacaggctc tatcttcata cattttcgca aataaacggt
     3121 caatctcatt ttgtctgttc tcggctttct tcaatgtact tgcctttttc ttcttttccc
     3181 gtattctctc tgcattgcca gctttctgta tcttgttcaa taccttttct tcgtcctgct
     3241 gtactgcctt agcccaatac tgcaagcgtt ccaatacggc ttgatacagc acatcataac
     3301 gaatgtagtg catagaacaa ttacctttgc caaactgccc gtagtaactg caagcataat
     3361 aactgtaagg cgttttattt gtcttattcg ttccaaatct catagaccaa ccacaatccg
     3421 cacacttgac aagccctgca aaaatcggcg ttgccttttc ctttgtctgt ctgcgtcttg
     3481 attttatctg ctcctgcaca cgatagaaca cttccttgtc aataatcggc tcgtgagtgt
     3541 tttctactcg aaaccattca ctttcgggct tacgcacttt ctttttgctc ttgaacgata
     3601 ccgtagactg catattgtga acactgtttc cgatataaac ctcacttttc aatatcgcct
     3661 tgacatgagc aatcgtccac tcataacgct tactttcggg ttttccctca aagatatgtg
     3721 caaaagtacc gtatcttgta aaatttagcc atgacgctgt cggaactttt tctgctctta
     3781 actgctttgt gatttttgca cttccgtaac cttgataggc tagggaaaag attttttcaa
     3841 taatccattt tgtttcatca tcaaccaaca gttttccttt gatgtcaggg tgtttcttat
     3901 atcctaacgg agcataagca ccgtaatgag ccccctccgc aaactttgtc ttaaatgctg
     3961 atttgacttt acggcttgta tctcttgcca cccattcatt gatgatgttc ttaaatggtg
     4021 caatctcact ttcgcctttt tcgctgtcta caccgtcatc aatcgctatg tagcggacat
     4081 tatgactagg gaaatacaat tctgtatatt gtcctgtcat aatataattt ctgccaagac
     4141 gggaaaggtc tttcgttaca acacagttga tttttcctgc ctctatatcc tcaatcattc
     4201 tttgaaaact cggtctgtca aaatttgttc ccgaccagcc gtcatcaata tattcatcaa
     4261 tgacattcaa atgatgttct tttgcatata aacgcaacat acttctctgt gtggtaatgc
     4321 tggaactttc gccctgtaat tcatcatctc ggcttaacct aagataaagt gcagtattgt
     4381 aaatctgttg tttcattgtc agttatcctc ctttataact aacccgtgtt tacaatacct
     4441 gcttgcaagt aaagtataac acgggtactt tttgtcattc aatctatttc ttacaactgc
     4501 accgatttta tgcgattgtc tgctttgctt tttccagcat gacatcagtt agaagttctt
     4561 ccattgtttt gcccttttcg gaaaaatgct cttttactac aatctttgtc tttcctctgt
     4621 tgccaatgcc acacttgcca tttttctttt gaattgtttt tacagcatta tcaataacac
     4681 ataccccctt tctgtaaaat ctttctaagc aattcaacgg cttttgtcat agcccacagg
     4741 agttccacct ctgcatagtt cttatgtagc cgtacccatt gcgtgcgacg ctcttaatgc
     4801 tcaagctgtg gctgtacggg agtatcatta tctgacagta caggtcatgg cgataaaaga
     4861 ggacaaatct tttacaagaa cacaaatagg ttttcgctcg ctcttgcagg ggaaaggtcg
     4921 tggcgtattt gtttcaacgg ctagctgtct gtcaaacgta ttgaatagct ttattcagtt
     4981 gtcaaagaac gattgaaaga aaaaaatcgt ctttcctata tatcgcgttg gaaaagagga
     5041 gaaacgtaac caaaatccaa aactttttcc attattagta caaatggaac ggttcatttt
     5101 ggcaatcaac ggttgatttt ctcctttttc actactatct gtacagcaaa cgtccatttg
     5161 ctaagtttaa atggaaaaat tttaacttct tttcatgcac catataggga tagaaaatgt
     5221 cattttgcta agttgatgat gaaaaaagaa tattttcctt ttcacaccct aactacccaa
     5281 accataccgt tactgccaac aatagaatga aatattttct ttgcaccata tagggaacgg
     5341 aaaccttaaa ttgttaagtt gagaacaaag tattttcatc ttttcacatc aacactcaac
     5401 tgctaatgcc tgtttgaata caaaatgatg atgtttttcc tcttttcaca gaagactgtc
     5461 cactaatcac gaatttccgt cattttttca aaactttttt cttctttcac ttcatatagg
     5521 gacagcaagc cccgatttaa tgacctattt tcaaaaaaag tttagatttc cttttctcgc
     5581 accatatctg tacgcaagta gccattttgt tgcagattta ataaaaaatt tatttttctt
     5641 ctttcacttc acatagggaa tgaaaacagc tctttgctga ctaaatctga aatttttcaa
     5701 ttacattaaa aaataccgtc attctcttgt ggaacagcgg tatctatacg ttcttctatg
     5761 gttgatttct ctaattgtaa taaatgttta tgcttgtctt ttagttctgc ctgctcaatc
     5821 atatctttca gtatcgttgt acgattttct ctgtctaatg cctgtaccat ttttatggtt
     5881 ggtgcgactt gtctttgtag ccaatgcaac gctttttgta aagtgtaagg ctctggcttt
     5941 gtggttaagc gtatcggctc tctgttttcc cctacaaacc atgcccaatc atcatttgtt
     6001 atccattgac tttttggctt gtcgtcctct ctgtcaacaa agcggacata atgattgatg
     6061 atagaaaaag cggtgcgttc agcgtcccga taggtcagta aatctacaac cgcataatag
     6121 gctcgttcgt tcttcatgcg tatttcaaaa cggttcttaa tctccgtatc ttcaatttct
     6181 gtgccattct tgacatactg ctcataattt ttctgataag cacacatata taattcgcta
     6241 cttgttgaac cgagatataa ggtttctcct gtattctttg gtaaatcgct accgcatttt
     6301 tctgtaccgc tgtaatcttt ctgcatacgg aagtaggaca tacattcgcc agccttgtat
     6361 ttttccttta actttggaat atccaatatt cctgctttat cattgatagc caaatcaagc
     6421 cgtttcatca caccaccagc cgtcatacaa tccagcatga agtcatacca tgagcgttcc
     6481 tgtgccagca gataacattc catttgccga cagcctttgc ctttcagttc caataaaaca
     6541 cctaaatgct cctgcatgga acacatgata ttgatgtcgc caagtacata tttgctttca
     6601 tatccgtatt ttccataatc ttcataaagc atgtaattag atttcagttg taatacatca
     6661 cggataatcg ccaatgcgtc cgtagtagga aagcggacac gaaagtaatc aatcaataag
     6721 aatagtggtt cttgcggatt gaaacgctca atctgttttt ctatgatttc tcttaattcg
     6781 tcattcaatg gctgttttcc tttttcaatg cggttgtaat attcacggct gataccacag
     6841 gcaacagcaa gccttgtttg tgttactccg tattcctcac gcttttgtct tagttcttca
     6901 ataaagtttt tatcattcat tcttcaattc ctccaatcaa aaaaggcaac taccataatt
     6961 gataattgcc tgtcattaaa aatctgtata gaaaaagcag tcaatcctaa gactaactgc
     7021 tttgtgtgta tggatataaa attgcttcta atcttgaata ggtatccaaa tttcaataac
     7081 tgaattatca ctattccaat gaaaacgata atcgtatttt tcaaagtgta ggataatatc
     7141 ttttatagga atatacgatg tactaggaat aatttcttca taaatcttgc cataagtttc
     7201 atatatttta tccatagcac caatatgctc cacaaccaaa tatttataag acggtatttc
     7261 tttatacaga aagccctctg gggtaatggt tctttgggga atagaacaga aataataaag
     7321 ttttccattt cttctttcca taaaagcata ttttacccaa tttcctgatt gtgaaagata
     7381 cgcttttttc aaactactg
//
LOCUS       DACEOE010000123         7352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEOE010000123 DACEOE010000000
VERSION     DACEOE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.125"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            21..3713
                     /gene="nifJ"
                     /locus_tag="IW774_002084"
     CDS             21..3713
                     /gene="nifJ"
                     /locus_tag="IW774_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1154419.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            3902..4789
                     /locus_tag="IW774_002085"
     CDS             3902..4789
                     /locus_tag="IW774_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1154420.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            4786..5955
                     /locus_tag="IW774_002086"
     CDS             4786..5955
                     /locus_tag="IW774_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154421.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            5979..7334
                     /locus_tag="IW774_002087"
     CDS             5979..7334
                     /locus_tag="IW774_002087"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1154422.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2391 a         1360 c         1708 g         1893 t
ORIGIN      
        1 atgaacaagg atgatgaatc atgcgaaaaa tgaaaacaat ggatggcaat acggcagccg
       61 catatatttc ttatgcattt accgagattg ctgcaatata tccaataaca cccagttcga
      121 ccatggctga attagtagat caatggtcag cagcaggaaa agaaaacgta ttcggtcaac
      181 cagtaaaagt agtagaaatg caatccgaag caggagcagc tggtgtggtc catggatcat
      241 tgaaaactgg tgcactgaca actacatata ccgcttcaca agggctgctt ttgatgatac
      301 cgaatatgta caagattgct ggggaactac tgccaagtgt tttccacgtt gctagtcggg
      361 cagtgacgac aaatgcgttg aatattttcg gagatcacgg agacgtgatg gctgcaagac
      421 aaacaggatt tgctatgtta gctgaaagca gtgtacaaga agtgatggat ttagcacctg
      481 ttgcccatct tgcttctatt gaaggagaag tccctttttt gaatttcttt gatggattcc
      541 gtacgagtca cgagattcaa aaaatcgaag ttttggaata tgaagaatta gctcctctga
      601 tcaatcaaga gaaattagct tcatttcgta aacgagggat gaatcccaat catccgactg
      661 ttagtggtac gaaccagaat ccagacatcc attttcaaca acgggaaacg atcaacccct
      721 attatgataa attacctgaa atcgttcaaa aatatatgaa agagatcaac cgattacgag
      781 gaacttctta tgacttggtg acttattacg gagcagacga tgcggaagaa gtcattgttt
      841 ctatgggatc agttgcacag acgattgaac aaacgattga ttacttgaat gcaaacggac
      901 ggaaaacagg ttttttgaat atccatcttt atcgtccttt tccagtggaa acattcgtgc
      961 aaaagttgcc tcatacagta aaaacaattg ctgttcttga tcgaagtaaa gaaccagggg
     1021 caggtggaga acctctctta ttagacgttc aaagtgcgtt atatgattct gaacttcgcc
     1081 cggctgttat tggtggtcgc tacggattgg ggtctaaaga tgtgactccc gaccaaatct
     1141 cagcagtttt tgatgaattg aaaaaagacc caagcatacg caaaaaacgg tttaccatag
     1201 ggatcgtgga tgatgtgact tatcaatctt tggaaccaaa ggaaagtctg gatttgacag
     1261 aaccgcaaac attccaggcc aagttttggg gatttggatc tgacggaaca gttggagcaa
     1321 ataaatcagc tatcaagatt attggagatc acaccgataa atatgcgcaa ggctattttt
     1381 attatgattc gaaaaaatct ggcggtctga ctgtctctca tcttcgtttt ggagatacgc
     1441 cgatacgttc agcgtatctt gtcgagcatg cggaccttgt tgcctgtcac acaccagctt
     1501 acctgcatag ctatgacttg gtcaaaggat tgaagccagg tggcattttt ttattgaata
     1561 ctctttggag cgacgaacaa ttagaaacgc atctgccctt aaaattaaaa cgttacttgg
     1621 ctgaaaataa cattagattc tatacgatca atgccatgag attggcccaa gaagtcggtt
     1681 tgggacgccg gatcaatacg gcaatggaaa cggctttttt taaattagct gacatcattc
     1741 cttttgatga ggtgcttccg ctgctaaaag aagaagccct gaaaagctat ggacataaat
     1801 caatggctat cgtagaaaaa aacatacaag caatcgatcg gacgaccgaa ctgcttcatc
     1861 aagtcgcgat cccagaccat tggagaacgt tggagatgcc tgaaaaaatc aataaaagga
     1921 acactggtta tatacaagaa atacttgaac cagtgaatgc acaggaagga aatcaactct
     1981 ctgttggtac acttgtagca aatggaatga caactggcga aatgccttta ggaacggctt
     2041 ctatggaaaa gcggggcgtc gctttagaag tacctgagtg gattagtgat cgctgtacga
     2101 tgtgtaatga atgtgccttt gtttgtcctc atgctgccat ccgtccattt ttagcagatg
     2161 aagaggaaat ggaagaagcg ccagaaggat ttatcgtgag agaaatgcga ggcgcagacg
     2221 gcgtaaaata ccgtatccaa gtttcagtgg aagactgtac aggttgtggt ctgtgtgtag
     2281 aggcttgtcc cgcaaaagaa aaagctttag tgatgaagcc ttatgaagaa cagaaagaac
     2341 aagctgtgaa ttgggctttt gcaatgacat taagacaaaa agcaaatcca gtcaaaggaa
     2401 agaatactgt cttgtctaca caattcaatc aaccgcttct tgaattctcg ggcgcttgct
     2461 caggctgtgg cgaaacacct tatgtaaaat tattgacaca aatgttcggt gatcgaatga
     2521 tgatcgcgaa tgcgacaggc tgttcttcta tttggggcgg tgcttctcct gcttctcctt
     2581 atacgaccaa tgaatgtggt caagggcctg catggtcaaa ttctttattg gaagataatg
     2641 ctgaatttgg ttatggtatg cttctagcgg cacagactcg tcgcgaggct ttggcaagtt
     2701 caatcaaaga agtattgcca aaagtatcag aagaactacg agaattgatg gaagactggt
     2761 tagtacacat gtttgagagt gaaggaacgc agcaaagagc agcaaaactg caagctgcga
     2821 tcgaaagcga acgatcaatt ttccctgaac tggaggatat atacaagaga aaggatctat
     2881 tcgtaaaaaa cagccaatgg atgattggtg gagacggatg ggcttacgac attggttttg
     2941 gcgggatcga tcatgtactt gccagcggag cagacgtgaa tatccttgtt ttggataacg
     3001 aagtctattc caatactggc ggtcaaacct caaaagcaac accagcttcg gcaatcgcta
     3061 aattttcggc tagtggaaag tatgcttcaa agaaagatct aggaatgatg gcgatgacat
     3121 atggaaatgt ttatgtggcg caaattgctt caggtgccaa tcaaatgcaa acgatcaagg
     3181 catttgaaga agcagaaaag tatcctggtc cttctctgat tattgcctat acgccttgta
     3241 tcacacatgg cttgcttggc ggaatgaaag aatcgcttaa agaagcaaaa gaagctgttt
     3301 cttctggtta ttggtcatta tatcgatata accctacaat ggtcgaaaaa aacaagaacc
     3361 caatgacttt ggactacaaa aaacctgatt tctctgctat gcctgatttc atgaggaaac
     3421 aagttcgctt ttcctcgcta gataacgcaa atccaaatct tgcaaaagct ttatttgaaa
     3481 agactgtcca cgacgctaaa acacgttttt acaactatgc tgcactgacg ggagaattag
     3541 aaaaaatcaa gaaacgactt gaaccagagg aatctatcga tgtacccgtt aagactagaa
     3601 aagaaaaaac gacggacctt gaagcagaag aaagaagagc tgctagaaga gctgccagag
     3661 cagaacgacg taaaaaaatg gaacaagcta caaaagaaaa aaaggaaaaa taacagaaaa
     3721 aatataatga ataattctct gaagtttaaa aatatttatt gcagagaaaa ataaaacgca
     3781 tacttgcaat tttcttgaaa attatgcgtt tttctcaaga aaaatacgag catatcagcc
     3841 tctaaaatta aaacatatga tatactagag aggaaatctc aaaagagaag agaggctttt
     3901 tatgtcattt caaatcgggg aactttttaa tttggactat tggcagcaaa tgttttccgg
     3961 tgacttgttc tctctgaatt ttttggtgaa catacttgat attctggttg tctggtatct
     4021 tgtatacaaa ctgatccaac tgctgcgagg cacaaaagcg attcagcttc tgaaaggtgt
     4081 agctattttt attgttatcc gaatcgtagc ggaaatcatt ggtctgcaca ccttgtcttg
     4141 gctgatgaac caagtcatta cgtacggggt gatagctgct atcgtgattt ttcagccgga
     4201 aatccggaga ggactggaac atcttggacg cagttcgctt ttcaaacaat caaaaagcga
     4261 acagcaggaa gatgagaaga tgatcttgtc ttttgacaaa gccatccaat atatgtccaa
     4321 gcgaaaaatc ggtgcgttga tcactatcga acgtcatact ggattagatg agtatattga
     4381 gacagggatt gctttagatg cagatatcac tggggagctg ctgatcaata tctttattcc
     4441 taatacaccg ctgcatgacg gtgcagttat tgtaaaagaa ggaaagatcg cagtagcaag
     4501 cgcctatctt cctctatcag aaagtatgtt gatccctaaa gaattcggga ctcgacaccg
     4561 ggcagctgtc ggtataagcg aagtcagtga cgcgattacc atcgttgtct ctgaagaaac
     4621 cggggacgtc agcatcacgt tagataatga actgatggct ggcttatccc agcaagaata
     4681 tttggcaatc ttgcgtagag aactaattgc tgaagagaaa acaaaaaaca agaaaaacgt
     4741 gattcaatct ttcctagacg gtatgactaa gggaggggat cgcaaatgat ctcaaaagag
     4801 aaacggacaa gcattcttta cagttttatt gcattgctgt tcagtattgc gctgtacttc
     4861 aatgcaaatg gccagtccgt tccaaatacg ctttctggaa atgaagcgta taatcaaaca
     4921 gttacggggg tgccgatcaa gatttcttat gattcaaagc gctactatat ccatggatat
     4981 gaaaatgcag tgactgtcaa actttccagt gcaaaccgag ttcagttgaa tgcagaggca
     5041 aacgaagata cacggatgtt ccgcgtcaca gcagatatca ctaagctggg cgaaggaaca
     5101 catgaagttc cgctaaaaat tcaaaatttg agcagtgctg tcacagcaaa gatcgaacca
     5161 tcgactatta cagtaacggt tgagaaaaag gtaataaaag atttcgatgt ggaaacgttg
     5221 attccttcaa cgttcacacc atctggatat gaacttgatg atacttccgt tagtcctaaa
     5281 aaagtctcta tcactacagg tgacaaaaca ttggcagaaa tcaaacgagt agtagctgat
     5341 gtggacgctt cgatggtcac agatgatggg attaatagtg aggtcccaat acaggcgctg
     5401 aatgcagcag gggaagtatt aagtatcgtc tctgatccag tgcaagtaac agtgagagct
     5461 gattcgatca agccaagcaa gtcggtacga ctttatggga tacagcaagg aacaccagct
     5521 gccggggtag aaagctatga tttcagtttt tcaaaactgg aagcagaagt aagcggctct
     5581 agcgatttgc ttgcatcgat cggtgacagt attgctgtac cgatcaatgt ctcaggtatc
     5641 aatcatcgaa caactcgtaa aattgaaata ccagtagaag aggggttaag tatcaagccc
     5701 gaatcagtta gtgtggagat tacacccgtg ttgcaaacgt caagcagtag ctctgcttcg
     5761 acgagcagtc ggacacaaga aaatacaagt acgagtagaa gtatttcatc tgctgattca
     5821 ggaacggaac catcgacaac aaccatttct tccagttcgg aatctgggac aagcagctcg
     5881 accacaagta cagaagaaac atcagaaaca agcgaatcaa cacaaatgag cagaacaaat
     5941 tcctcagaaa attaagttaa aggagtaatt ttacaactat gggtaaatat tttggaacag
     6001 acggcgtacg cggtgaagcc aacaaggaac ttacgccaga acttgcattt aagctgggac
     6061 ggtgcggagg ttatgtccta agccagcatg aagaagaagg aaaacgccca cgcgtgttag
     6121 tagggcggga tacaagaatc tcaggtcaaa tgttagaaga agcgctgatt tcaggacttt
     6181 tatcggtggg tatcgaagtg ttccagctag gtgtgatctc tacaccaggt gttgcttatc
     6241 tgacacgttt gcaaaaagca agcgcagggg tcatgatttc tgcttctcat aatccagcac
     6301 aagataatgg aatcaaattt ttcggtgcag atggttttaa actagtagat gaccaagagc
     6361 tagaaattga agcattatta gatgctgaag tggatgaatt gcctcgtcca tcagctgaag
     6421 gattgggaac tgtagaagaa tttccagaag gcttattgaa atattctcaa ttccttgtac
     6481 aaacgatcaa cggtgacctt tccggtttga ccatttgtgt cgatgcagca aacggagcaa
     6541 cttcaacaag tgttaatcgt ttgtttgccg atcttgaaac agatttttat acaatgggca
     6601 caaatccaga cgggttaaat atcaatgacg gcgtaggctc tacccatcca gaacgtttag
     6661 ctgagatggt cgttgaaaaa ggagcagatg ctggactagc tttcgatggc gatggcgatc
     6721 gtattattgc ggtggatgaa cttggaaaca ttgtcgacgg tgataaaatc atgtttatct
     6781 gtgctaaata cttggcagaa aaaaatcgat tgaaaaaaga tacgatcgtt acgactgtca
     6841 tgagtaactt aggattccac aaagcggtgg aaggaatcgg tttgaaagat gtcgttacac
     6901 aagttggtga tcgttacgtt gtagaagaaa tgcgcaaaaa cgattataac tttggcggcg
     6961 agcagtctgg gcacatgatt ttcttagatt ttaacacaac tggagatggg atgctttcag
     7021 gaattcagtt actgaatatc atgaaacaga caggtaagaa attatcagag cttgcagcag
     7081 aggtaacaat ctatccgcaa aaattagtga atatccgcgt aagtgataaa aacggtgcga
     7141 tggatgttcc ggcaatcaaa aaagtgattg aagaggcaga agctgaaatg ggatcagaag
     7201 gaagaatcct agttcgtcct tccggtacag aaccattact tcgagtgatg gcagaagcgc
     7261 ctactgatga gaaagttaat tactatgtcg ataaaatcgc ggcagtagtc aaagaagaaa
     7321 tcggactgga ttaagagata ttttcataaa aa
//
LOCUS       DACEOE010000124         7302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEOE010000124 DACEOE010000000
VERSION     DACEOE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.126"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            99..746
                     /locus_tag="IW774_002088"
     CDS             99..746
                     /locus_tag="IW774_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1154423.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IW774_002089"
     CDS             1139..3784
                     /locus_tag="IW774_002089"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1154424.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IW774_002090"
     CDS             4094..5407
                     /locus_tag="IW774_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1154425.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IW774_002091"
     CDS             5397..6050
                     /locus_tag="IW774_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1154426.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="IW774_002092"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="IW774_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ1154427.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6671..>7302)
                     /locus_tag="IW774_002093"
     CDS             complement(6671..>7302)
                     /locus_tag="IW774_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1154428.1"
                     /translation="YAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVAGQTIKARYK
                     KKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKFEQIQINYSK
                     KQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNEIEVEPAKID
                     IKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI"
BASE COUNT         2370 a         1235 c         1528 g         2169 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata atctttggtt gtttttttga atagtttatt tgtatttgtt
     7021 caaatttttg tttagtagct tcaaaaatat aacgttctaa atttttttcg taaaaagcgc
     7081 gcttatttgt gcaacgtaat ttattatttc ttcgttttac acatctataa caagtccttt
     7141 cgtatataga ttctgtaccg tcttttttct tatatcttgc tttaatagtt tgaccagcta
     7201 ctgatccctg acagtcagaa cattttaata aacctttaaa aatgtaatca cgttttgcat
     7261 ttgtttttaa atttttaggt aaggcttttt gaacagcata aa
//
LOCUS       DACEOE010000125         7298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEOE010000125 DACEOE010000000
VERSION     DACEOE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.127"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            138..458
                     /locus_tag="IW774_002094"
     CDS             138..458
                     /locus_tag="IW774_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154429.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            910..1110
                     /locus_tag="IW774_002095"
     CDS             910..1110
                     /locus_tag="IW774_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1154430.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1257..4046
                     /locus_tag="IW774_002096"
     CDS             1257..4046
                     /locus_tag="IW774_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1154431.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4093..4620
                     /locus_tag="IW774_002097"
     CDS             4093..4620
                     /locus_tag="IW774_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1154432.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4641..5831
                     /locus_tag="IW774_002098"
     CDS             4641..5831
                     /locus_tag="IW774_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1154433.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5871..7169
                     /gene="asnS"
                     /locus_tag="IW774_002099"
     CDS             5871..7169
                     /gene="asnS"
                     /locus_tag="IW774_002099"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1154434.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         2422 a         1338 c         1566 g         1972 t
ORIGIN      
        1 aaagaggttg tgacgtggtc acgacctctt tttgacgatt atgatacaaa aaacatcatt
       61 tatgaacaaa atgtgaataa cgggcgttct tgagatatga ttttaattgg gaaaaactta
      121 tcgataagga ggtctttatg aaaaaaagac tgttatggtg tttgctgttt ctatccctaa
      181 gtttaacaag tcagccagtt tttgctgaag aaacacagcg gtctcctatt acaggcacac
      241 gtctgtacaa aaatgtttat gtacagacaa cagatcttga ttctgcgatt cctgaaacag
      301 aagaacggtc tttgcagcca agatttggtt acttttggta ctgtccgacc tgtgattatg
      361 aaagttgctg gcatttctta cattctacag ctgcagcttt tgcttttact catactcaga
      421 aatatgatgg acatcatcct gttatttatg aggtatagat atgagcaaaa aagaaaaact
      481 aatctcgttg atcgtattgg tttgccttgt tcttctggta actgtcatag gctatcagaa
      541 ataccatgag aacatcagac ctactaaaga atcttcccca gcctcgaccg ttgaaacaga
      601 accgataagt agtcagaaag aataattttt ttagtttaaa gaagaacatc agagcaaaaa
      661 aacaccaact gaacaatatg tcgtttattt taaaaaaagt tcggtttttc actttatgta
      721 atcgctgaga aaactttttt taataaaaaa agtcgaaaaa ctctagcttt tttgtaaacg
      781 gtatgatata ctataggagt agtttttgtt cggtaatctg tggtagcttg tttcaaataa
      841 acatcttcca gatatacacc attcaagtaa ttgcaaaata tgtgttgata cacaaggagg
      901 atttttatta tggaaacagg tacagtaaaa tggtttaact cagaaaaagg atttggattc
      961 atcactgcag aaaacggaaa cgatgtattc gttcatttct cagctatcca aggcgacgga
     1021 ttcaaaactt tagaagaagg ccaagcagta actttcgatg ttgaagaagg ccaacgcgga
     1081 cctcaagcaa caaacgttac taaagcttaa ttaacctata ctaaaaaata aacgcccaga
     1141 catttgcgtg tctgggcgtt tttgtctatt ttctttaaaa agcgatgaga gtatttttct
     1201 gcactttgta gtataatttc aaaagaacat tagaggcagg agaagaggaa gatattttga
     1261 aaccaagaac ttatgcagtc gttgatatcg aaacgactgg gacgaatcca aaagaagatc
     1321 ggatcatcca atttggctgc gtcatgattg aatcaggtcg gatcacttca cgtttttcga
     1381 tcgatattca tccagggcgg aagatttcaa aacaaatcca gcatttaaca gggatcacga
     1441 atcaacgcgt ccaaaaagcg ccttatttcg aagatgtcgc ccaaacgatt tacaatcttt
     1501 tggcagacac catatttgtc gcgcataata tttattttga ttataatttt ttgaaccatg
     1561 aactcatgcg atgcggactt cctagtttga agataccagg catcgataca gtagaacttg
     1621 cacaaatttt cttgccgacg gaaccaagct ttcgcttagc tgatttgtca gaaagccttg
     1681 gatttattca tgagaatcct catcaggcag atagtgatgc cgaagtgacg ggtcaacttc
     1741 ttttgctgat cgaagaacgg atgagaaaat tgccaatcat cacgttagag cagattgcgc
     1801 gattaagtag acacacaggt atggatacga gtcgtttcat ttatcatgtg attgaagaaa
     1861 tgaaagaaaa accagagcca cttgatccct cattagaaat cgtcgatggg ctagctttgc
     1921 agaaaaaaga agtggaacta ttcacatccg tccattatgg cgagcggaca tatccaagaa
     1981 aaaaacaagc aaaagaaaaa atgtttggta aaacactgat gtatcgaaaa gaacagaaca
     2041 gactgatgaa tgcggtctac gaccatttca caaaagatga atcaaaagat ttgatgatcg
     2101 aagcagctac aggaatgggt aaaacaatcg gttatctgct gccattgagt tatttggcta
     2161 cacctgaaaa acccgctgtc atcagtaccg tttcgctagt tttgcagcag cagatcatag
     2221 aaaaagacat tccattgctg aatcaattgc tagatcagcc catacaacct gtaattatca
     2281 aaagctatcg ccactatatt gatttacagc gttttaaagg cacacttgtg gaaccgccag
     2341 aacaaaaaca atatgctttg tatcaaatgg cggtcttagt ttggctgacg caaacgaaga
     2401 ccggtgattt ggatgaatta catttaacaa atttgaacca ttcattcttt gcagatatcg
     2461 cgcacagagg tattggattt ttagcaagaa atcagtcatt ttacgaacaa gactttgttc
     2521 gtttcttgca aaagaaaatc aggcaaagta attttttgat tgtcaatcat gcgtttttaa
     2581 tacaagaaac acagcgaaaa gaaccattgc ttccagctag tccatttttg ttgatcgatg
     2641 aagtacatca tttgccggaa atcgctgaaa aagtaaatga caaacgatta agtattaact
     2701 atttccataa acaaatccag caattagaag aaccaaatca gctgtttgac cgaatacaaa
     2761 gctatttacc gaaagaacat gaagccttac gctggttatc gatttatcga gaagaacttt
     2821 ctgcgctatg cgaagcacag atttgtatgg cagaaggatt aatgagttta gtgagcggca
     2881 agcaacctaa cggacagtat ccagaagaag tcatgatgac aaaagagatg ttggatcagc
     2941 tgccactgga atctgggaca gccattgatc acctgcagct tttttatcag gagatccagc
     3001 agctacagtc acaaatgcgt ctgacttttg agattatcca agaaagctgg acaaaccaga
     3061 tgcggatcga tttagcaagt ttatatagct tatttgatca attagacaag cagtacttgc
     3121 tgatggatca atggctggaa gactggcgag atcatttgat ccattggttc gttcctggga
     3181 acacagcgca gcagatgatt tttcatctcc atgattttca agcggctatg ttgccgtcga
     3241 ctgtttggta tcctcgatat gatcgtattc tttatattgg aggaacgctg aagatcggcc
     3301 ctaatcgaca ctatctctct aagcggcttg gcattgaagc agcacctctt aaagtgattg
     3361 cctctcctta tgattatgag aaacaggcaa ggatctatgt gccaaaagag gcacctgccg
     3421 tccatcaagt cagcaatcaa gcatatatcg attatttaga gacagcactt acaggtctga
     3481 tcgaacaaga agatcggcca atacttgtgt tattcacttc tcatgatgtt ttgcagaagg
     3541 tctatcagaa aatgcatctg tctatgctag aaaaaggacg agaaatacta gctcaagggt
     3601 ttggcggaag ccgggagaag cttttgaaac gcttcatttt gtcagaaaaa agcgtattgt
     3661 ttggcgctga cagcttttgg gaaggtgtag atttgcctgg ggatgctttg cagatcgttg
     3721 tagtcacacg tttacccttc gataatccgc ttcgcccgat ggtcaaagct aggaacgctt
     3781 atttagaatc agaggggatc aatccttttt accaggaatc cgtacctaaa gcagctttga
     3841 agttacgcca agcattaggt cgcttgataa ggacggagac agatcgtggc gtattgctta
     3901 ttttggatcg aagactagtc actgcaaaat atggtgcacg gatcatccat gcattaccga
     3961 aaaaagtacc gatcaaagag ctagctatgg aggaaatcta tcaggacatc catgaattct
     4021 taaagaaaaa tgaagaagga agttagagga gctgtctttt ttcataatta aaatctgcta
     4081 taatgaaaag gaatgaagat aaaggagcga cgacccttga agaaaaaaca gacaaaggaa
     4141 aaaacgatta atcgcgtgtt acttggcttg atcgtatttt tattagcggt catcgtgatc
     4201 agctcgattt tttacttgcg ttccagtcgt ccggcggtgc aggcgaagaa agaagcagta
     4261 gaaatcgcaa agaaatatgc aaatcttgag gaagccgatc atttttattg gtttactcga
     4321 aagaagacat acttttcact gacaggtaca aatgataaag gaaaagaaat cgccgttatc
     4381 attcctaaat ctggtgaaaa agtaaaagta ttggatcaaa caaagggact gactgaaaac
     4441 gaagcaaaac agacaattgc tgcggcccat cctgaaatcc aaatcgaaaa agctgcttta
     4501 gggatgtatg atgatcagcc tgtgtgggaa gtagtaggaa aagaaacaaa tgggaaactg
     4561 aattattacc tgattggctt tgaatctgga gatgaagtca agacaatcaa gggaatttaa
     4621 aaggtaaagg aatgaatggg atggagttat caaaaagaac aaaacgatta gaaccgtctg
     4681 tcactttggc agctgcggca aaggccaagg cattaaaagc aaaaggcaga gatgtgctga
     4741 gtttgactgt gggagaaccg gattttgcga caccagaaaa catccaagaa gcagcaatcg
     4801 aagcaatccg caacgggaaa gctagctatt atacaccaac agcaggaatc ccagagcttc
     4861 gacaggcaat cgttgattat ctgaagaaaa actatggact tgtctatgaa ccttcacaaa
     4921 cagtcgtaac agacggagcg aaatttgctt tgtataccct tttccaaacg atccttgacc
     4981 cgcaagatga agtcatcatt cctgtgcctt actgggtcag ttacggtgag caagtaaaac
     5041 tggcagaagg acttcctgtc tttgtgaaag gggaagaatc caatagctgg aaagttactg
     5101 ttgaacagct agaacaagca cgttcagaaa aaacaaaagc cgtaatcatc aattctccat
     5161 cgaatccgac tggaatgatt tatagcaaga atgaactgca ggcaatcggt gaatgggcag
     5221 taaaaaatga tattttgata gttgctgatg atatttatgg tcgtttagtt tataatggca
     5281 atgagtttac tccaattgca acgatttcag aagcaatcaa aaatcagact atcatcatca
     5341 acggcgtgtc taaaacatat gcgatgacag gctggcgcat tggttatgcg gtgggaaatc
     5401 ctgtgatcat caatggaatg atcgctgttg cttcccagtc aaccagtaat ccgacagcag
     5461 tcagtcaata tgcagctgtt gaagcattga aaggcgagca agatacagtg gaagaaatgc
     5521 ggaacgcctt tgaagaacgc ctgaatacgt tatattcgct tgtggcagaa ctgccgggtg
     5581 tttctcttga aaagccacaa ggagcttttt atctttttcc taatgtaaaa gaaacgctac
     5641 gcatgtgtaa atatgaaaat gtcacacaat gggtagaaga tcttttagaa gaaacagggg
     5701 tagcgcttgt gactggcgaa ggctttgggg caccagaaaa tgtccgcatg agttatgcta
     5761 ctgatcgcgc tacgttagaa gaagcggtac gcagaatcaa gcaatttatt gagagtaaaa
     5821 gtcaaaatta atcctaaaga aattcaagga atataaaagg agagtcatac gtggaacaaa
     5881 ttcaaatcat cgattcgaaa aaccatgtgg gagaaacagt aacaatcggc gcttgggtcg
     5941 caaataagcg atcaagcgga aaaatcgcat ttttacaatt aagagacgga acagcctttt
     6001 tccaaggtgt agttgtaaaa agcgaagtgt cggaagaagt tttccaaaca gctaaaaatc
     6061 tacaccaaga aacatccgtg ttagtaactg gagaaatccg tgaagacagt cgttcaaaat
     6121 ttggttatga aatcggaatt acaaatgtgg aagtggtaag cgagagccac gattatccga
     6181 tcacaccaaa agaacacgga acagacttct tgatggatca tcgtcattta tggctgcgtt
     6241 cttcaagaca acatgcgatt atgcagatca gaaatgaaat cattcgtgca acgtatgaat
     6301 tctttaatca acatcatttt gtcaaaattg atccgccgat cttgactggt tctgcaccag
     6361 aaggcactac ggatctattc gaaacaaatt actttgatca aaaagcttat ttatctcaaa
     6421 gtggccaatt atacatggaa gctgctgcta tggctttcgg aaaagtcttt tcattcggtc
     6481 cgactttccg tgctgaaaaa tcaaaaacac gacgtcattt gatcgaattc tggatgatcg
     6541 aaccagaaat ggctttcact catcaagaag aaagtttaga agtacaggaa caatatgtag
     6601 cattcttagt acaaaatgtt ttagatcatt gcgaatatgc attagatgta ctaggacgag
     6661 acaaagaagt cttaaaaact tatacaaaat tgccattccc tcgtattact tatgatgatg
     6721 cgatcgaatt actgaagaaa aatggatttg atgacatcga atggggcgaa gactttggtt
     6781 cacctcatga aacgttcatt gcgaattcat tcgaacagcc tgtctttatc ctaaactatc
     6841 caaaagccat caagccattc tacatgaaac cacatccaac aagagaagac gtggtgatct
     6901 gtgcagatat gatcgcacca gaaggttacg gtgaaattat tggcggaagc gaacgggcaa
     6961 cagactatga tttcttgcta gaacaaatcc gtaatcacgg tttggatgaa aaagaatatt
     7021 catggtatct tgatctacgt aaatacggtt ctgttccgca ctctggtttt ggtctaggtc
     7081 ttgaacgaac agtcacttgg attgctggta tcgaccatgt acgtgaagct attccattcc
     7141 cacgtttgtt gaaccgaatt tatccataag aggtgatgaa aaagctgttc tgcatcaagt
     7201 aattagaaca gccaagcaaa aacagctgtt catgtttttg aattggcggg acttattacc
     7261 gcagatgtaa gcttttgaat accgtttagt ggagaggt
//
LOCUS       DACEOE010000126         7296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEOE010000126 DACEOE010000000
VERSION     DACEOE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.128"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            167..796
                     /locus_tag="IW774_002100"
     CDS             167..796
                     /locus_tag="IW774_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154435.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(920..2029)
                     /gene="ugpC"
                     /locus_tag="IW774_002101"
     CDS             complement(920..2029)
                     /gene="ugpC"
                     /locus_tag="IW774_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1154436.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2238..2660
                     /gene="mgsA"
                     /locus_tag="IW774_002102"
     CDS             2238..2660
                     /gene="mgsA"
                     /locus_tag="IW774_002102"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1154437.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            2983..3519
                     /locus_tag="IW774_002103"
     CDS             2983..3519
                     /locus_tag="IW774_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1154438.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(3559..5322)
                     /locus_tag="IW774_002104"
     CDS             complement(3559..5322)
                     /locus_tag="IW774_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154439.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(5325..7040)
                     /locus_tag="IW774_002105"
     CDS             complement(5325..7040)
                     /locus_tag="IW774_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154440.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         2197 a         1391 c         1429 g         2279 t
ORIGIN      
        1 tttgtcacat ctcctatttt tgtttcttga tcaaaaaatt cttatacttg atactcacac
       61 tcattttgta tggatttaga aaagaatatg tgtcttgaca gttattagta agtgagttat
      121 actaagtcga catttatgtg tcaagacata ttaggagaga gaaaaaatga gaaaaaattc
      181 agttcgaaaa ctaacgattt ctgctttatt gattgggatg ggggtcatta tcccaatggt
      241 catgcctaaa atcatgattc caccagcttc ctttactttg gcgtctcatg tgccattatt
      301 tattgcgatg ttttttacac caggagttgc agtggctgtt gcgctaggta cgacatttgg
      361 tttcttttta acaacgccgg ttatcatcgc attgcgagca ttgtcacatc ttgtgtttgc
      421 gttgattggg gcttggtact tgcaaaagca tccgagtatc gttttaaaaa atggacagtt
      481 tacttttgct aattggcgtt tccaagggtt caattttgtg atcggtgtga ttcattcttt
      541 agcagaaatg ctagtagtca gcatctttta ctttattggt aatatgcctg aaacatatta
      601 ttctcaagga tatttttata cagtattcat cttgatgggg attggtgggc ttatccacag
      661 tttagttgat tataatattg cttattttat agcaggaaca ctcagcaaat attttgatat
      721 ccccgtattt acggcagcaa aaaaagaaaa tcggaaagag tcgattcaag ggtacgcgaa
      781 gaaaacgaca tattagacat gatctctcaa aaaaaaaact ctcatctagt aatggataat
      841 aaatcctcta agatgagagt tttttgtcct aaaaagagct gcaccaacat cttctggcac
      901 agctctaaca gggggcaatt tatggaagtt tgatgacttg ttctgtttct ttatcaaaga
      961 agtggccttt gctgatgttg aaggcaaggt cgactatttc gcctggttta tggaaatctc
     1021 ttgcatctac ttttgagatg aactcagtag aaccagtttt tgtgtaaagc atcgtttcag
     1081 ctccaagtaa ttcggaaacg acaacttctg atttcactac ggcttcaggt gaagcttcaa
     1141 gggctacttg ttcactgtgg atgtcttctg gacgaatacc gaagataact tcttttcctt
     1201 cataaccttt ttcaactaat actttgtttt ttccttcagg aatacgaagc ttaagcccat
     1261 gtccatctgt gataacacca tttttcaatg tcacattaaa gaagttcatg gcaggagatc
     1321 caataaagcc ggcaacgaac atattgacgg gtgtatcata aacttctttc ggtgtaccga
     1381 tttgttggat aaatccgtct ttcatgataa cgattcggtc agccatagtc attgcttctg
     1441 tctgatcgtg agtaacatag atggtcgttg tttcaagacg ctggtgaagt ttggcaatct
     1501 cagcacgcat ggctacacgt aatttagcat ctaaatttga taatggctca tccattaaga
     1561 agacttttgc atcccgaacg attgcccgtc ccaatgccac acgttggcgt tgtccaccag
     1621 ataaagcagc tggttttcgt ttcaagtatt ctgtcagacc taagatctct gctgcattgt
     1681 ccacacgttt tttgatgtct gctttatcat atttacgtaa tttcaagcca aaagccatat
     1741 tatcaaaaac agtcatatga ggatacaatg cgtagttttg gaaaaccatg gcaatatcac
     1801 ggtctttagg tgccacgtca ttcatgactt ttccgccgat atttagttct ccttcagaga
     1861 tatcttccag tcccgcgatc attcgcaaag tagttgactt tccacaaccg gaaggaccaa
     1921 caaaaacgat aaattcacgg tctttgattg atagattaaa atcagttaca gaataatttt
     1981 ctgcattgtc atatttttta tatacgtttt tcagagccat ttctaccata atacgttcca
     2041 cactttcttt tttagtttta ttaactattt aaagtataaa tgaaagcgtt aactaacttc
     2101 aatgtcatgt tgaacaagaa ttttttcttt tttttgtcaa tgtgctagtt ttaagcttta
     2161 ataaatatcc ataagaaata aaaactgata cactggagta aataagtatg ttaacaatat
     2221 caaaaggagt gtctcttatg aaaatcgctt tgatcgctca tgaccgaaaa aaacctatga
     2281 tggtgaaatt agcaacagcg tataaaacaa tattggaaaa acatgaactt tttgctaccg
     2341 ggacgactgg gcagaggatc acggaagcaa ccggcttgcc gatccatcgg tttaaatccg
     2401 gaccgttagg cggggatcag caaattggag ccatgatttc ggaggatcag ttagatttgg
     2461 tgatttttct tcgagacccg ctggctgcac agccccatga accagatgta acagcactta
     2521 tccgtttatc tgatgtctat gagattccgt tagctacgaa tatcggaaca gctgaggtgc
     2581 tactaagagg actggaagcc ggcttcctag attttcgaag aatcgtccat gaaatagaca
     2641 agcatccact ttcattttga tggataagcg tatggaaaaa taacgtttaa tgttcgccta
     2701 tatgtagcta taaaagctaa acaaactgat aatatgcgat ataatacgaa cgtattggat
     2761 aatataaaca ataatattag tttttcgtat ttgcagtaaa tgtgaaaata tgctatagtt
     2821 tctgtgggaa aagatcccaa aaataataca gagtagaaaa taaagcgtta agtgctaaga
     2881 ggatgggatg ttgcctcttg gacgaaggac gtaatgttcg cggttttatt ttcgcattcg
     2941 ctgcatgata cgatgtagca gctcttaaag gagatgctaa aaatgaagaa acaccggtta
     3001 gacgcacgta tgattgctat tatgggctta ttgattgcat tgatggtcac tttgtcccga
     3061 ctggtagcaa tcgagacccc atttattaaa atcagtgtca catttattcc acaagtgatc
     3121 atgggcattc tttttggtcc tttttggtca gggataggcg cagttttagc tgatttagta
     3181 ggaatggccc tcttctcgaa atctgcattc tttattggat ttacattgaa tgcatttatt
     3241 gaaggagcaa tctacggatt ctttttctat cgaaaagaga tcacttggaa aaatgccatt
     3301 ctggctacgc ttagtgtcac gctgatcatc aacttatttt tgacaccatt atggcttgcg
     3361 ttgatgtacc atgtgccatt gttcagctgg gtcgtttggg cgccccgcct gttaaaaaca
     3421 gtaatatggt taccgattca atcaattgcc atttattacg taggacgtag tattccttac
     3481 aaaaaaattt taagaagttt agcgatccac gctaaataaa aaacaagcag tcttcccatc
     3541 ccagaagact gcttgttttt attgtgctgt gaattgtgct tgatagagcc gtcggtaata
     3601 accatctttt tgaagcagct catcatgggt gcccatttcg acaatagctc cctgatccat
     3661 cactaatatc tggtccgcat ttttgattgt ggacaggcga tgggcaatca caaaactggt
     3721 ttttccttcc atcattgcta agaaggcttg ttggattttt ttctctgtca atgtatcgac
     3781 tgagctggta gcttcatcta agatcaacat agggggatta ctgatcatcg ttcgagcaat
     3841 cgtcaacagt tggcgttgtc catctgatat ctttaaccct tgaccgccaa tcttagtatc
     3901 taatttttcc ggcaaacgtt ctacaaattc atacatataa gaggctttta gggcatcatt
     3961 gatttctgcg tcacttgctt ccggattccc aaaacgaaga ttttctcgca atgtactgtc
     4021 aaatagccaa gtatcttgta acaccattcc aaaactttta cgtaatgtat cacgttggat
     4081 agagcggatg tcatggtcat cgactgtaat tgttcctttt gttgtgtcat aaaaacgcat
     4141 cagcaaatta acaagtgtgg attttccggc ccctgttttt cctactatag caatggtttg
     4201 acctggataa gcagtaaaat tgaaatcttc gatcaaacgc tgcttggggt cataagaaaa
     4261 agaaacatga tcaaaagaga cttttccttt gatatttttg ggttccaagg cataggcatc
     4321 tggcaaatca ggcttttctt ctggctgatc gatcaaatca aatgctcgtt ctagaccaga
     4381 aaaagcggtt tgcagttgtg tcgtgattcc agaaagttcg ataaatggtt tagaaaactg
     4441 acttgagtag atcgtaaagc tggaaatcac accaacagtg attgacgaac cactcttcaa
     4501 tgccaatagg ccgccaacta aaccaatcga taaataagca aggtgatcta caaaacgaga
     4561 agacgggttt gtcaaggaag aagaaaattg tgcccgttgt cctttttcat atagatcagc
     4621 atttagcagt tcaaattgct gttgactagc ttcttcttgc tgaaaagctt tcacgatttt
     4681 ctggttaccg attcgttcgg tcacaaagcc cgagatatcc ccaataatcc tttgctgtct
     4741 atcgaaattt gtttgtgaag cacgagcaac gatccaactc acaaggaaaa taattggtgt
     4801 agaacaaaga acgaccaatg tcagaagtgg acttagacgc atcataaaaa taaacgaaag
     4861 aatgatgatt gccactccag aaaataattg attgaaggcc gcagaaattg caatggaaat
     4921 attgtccata tcatttgtaa atcgactgac gatatttccg tgggagtttt gatcataata
     4981 acgaagtggc aatcgattca gatgagcaaa tgcatctttt cttaattttg caacggataa
     5041 gtaagctaac cgattgctta acacttggat gagccattga ctgactacgt tgattaaaac
     5101 gataccaccg aatagaataa gtaaatggaa cagtcgattg aagcggacct gtccttctcc
     5161 aatcatttga tcgatacttt gaccaatttg taacgtcatc acaactgttg caatcccatt
     5221 gatgattcct agtatcacag ctagccaaat gtcttttgca taggcagtaa ggtaaggaat
     5281 gaatcggcgg agcgtctgca aggataattt ttttactttc ataactattc ttcctcctgt
     5341 gatgcgacga tttcctgata ttctgctgat tgagcaagta attcttcatg ggtaccctga
     5401 gcagcaatct taccttgatc catcactaag atatgatgtg cttcttgaat agagcggaca
     5461 cgttgggaga taataatcac tgtactgcca agataagtct gtagcgcctt acgcaaatcc
     5521 aagtctgttt gataatccaa cgcacttaaa gagtcatcta aaatcagtaa tgctggcttg
     5581 gcaatcaaag ctcttgcaat cgtcagtcgc tgtttttgtc cgccagagaa atttttccca
     5641 ttttccatga cttttgtatc taaaccattt ggaagctgac ggacaaattc ttctgcctga
     5701 gcgattttca gagcttccca acattctttt tctgtggcgt ctttcttacc ccattgaagg
     5761 ttttcacgaa tagtcccggt aaatagtaca gaagtttggg gaacaagagc aatttgctga
     5821 cggagatgat ccagctgcca agaacgaaca tccattccgt ccattataac tgtaccggaa
     5881 ctgacatcat agaaacgcgg aatcaattgt gtcaaagtcg ttttcccgct tccggttgga
     5941 ccaatgattc caaggactgt atttcgcttc agctgaaatg taatattttg gagagaaaga
     6001 ccagaatcag gagtataacg gaagtcaaca tggtcaaaag cgataattgc ttcagaagaa
     6061 aaatcgggag tagtactgac ctcagcttca agaatcgaat tttctgtgtc tagcacctct
     6121 ttcacccgat ttcctgaagc ttcggcacgt gtgaagatca cgacgaggtt tgaaacaacg
     6181 atcagtgcca aaagcatttg attcatataa ttgatcaaag caagaacttg tccttgttgc
     6241 aagctaccaa tatttacttt gatcccgcca actgtcagca aacagatgat cccgacattc
     6301 atgatcaagg ttgtagcagg tgataatagg gcagagatat tggaaacacg taaataattg
     6361 ttcgctagat catttgtgct tttatcgaac ttctctgttt ctttttttgt ctgcgcaaat
     6421 gcgcggatga cacgaacacc gctaagattc tgactaacgt gttcattgag ctgatctagt
     6481 ttttcttgca ctttttggta gagaggaacg gtttttttga tgataaaaaa taaaatgatt
     6541 gagaaaatag gcaataatgc taagaaaaac agacctactt cccagtcgat cacaaaagcc
     6601 atgaccactg acccgatact taaaaacggt gctctgatca ataaacgaat gaccattgct
     6661 aaagctagct gaagctgatt gatgtcgttt gtcattcggg tgatcaaggt atctgttcca
     6721 aaatgattca actcgttgtg agagaaagta ttgatttttt tgattagctg attacgtaac
     6781 tctgtaccaa atccttgtga agctactgaa gcatagtatt ggcaaatgat agcacaaatc
     6841 agaccaatca cagacatagc gaccatccat aagcccattt caatgatttt tcctcggtca
     6901 ttcattttta gtccattatc gatcaaggaa gccatcatta aaggcaggac aagttcgaag
     6961 acagcttcaa gaaatttgaa gaacgggccc aaaatgattt gcttacgata gtcttttgca
     7021 taacgtaata actgaatcat atgattcctc ctattattta tttgtccaca attgaaaaaa
     7081 caagtactac cacattgtag cttaggaaat ttagaatgaa aagagaacaa gccttttcat
     7141 aaatgaaagt tttattatga agaagcttta gaaattgaat agactatttt ttttaaaaaa
     7201 aagaaaatga gaaaacatgc cagtctcttc attcatagga acgtctattt tacttaattt
     7261 ttctataaat gaattctgca taaaaaatat atcaag
//
LOCUS       DACEOE010000127         7266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEOE010000127 DACEOE010000000
VERSION     DACEOE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.129"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2498
                     /locus_tag="IW774_002106"
     CDS             <1..2498
                     /locus_tag="IW774_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1154441.1"
                     /translation="EQPFFAQPKDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLI
                     TGESGTGKSYLVNLVYQYCLLHDLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAY
                     TGAEENKKGAFEAADGGILFLDEVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKT
                     RLFFATTEEITSHFLTTFIRRIPIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPL
                     SVSGPALSLLTSQKLSGNIGELKNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQP
                     ITKNTAQQGIYITQDQTLEQLMEKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLK
                     QEVIQLFDFLLFETSRQEKHELLVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLY
                     QRSTIKWLPEEHDIIQLIKELEQQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRIL
                     LTIYLKKAKWSKEKRLPKAMIAAHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQ
                     IASEILDYCENSDVSNGLIILVDMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGE
                     CLQKNVSLEEIAEETVRQIQPEWRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSL
                     PTACQLKIIPYEYQQLKDKKNSDPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEK
                     MSVLAEWLDTTMNASEKEIFNQRIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEV
                     QLNRSISNPKKLALYVHVSCLIERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVI
                     EEHYSVMIPNSEIAYIYDILSEDTEFLSIEEEF"
     gene            2579..2998
                     /locus_tag="IW774_002107"
     CDS             2579..2998
                     /locus_tag="IW774_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1154442.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3010..3480
                     /locus_tag="IW774_002108"
     CDS             3010..3480
                     /locus_tag="IW774_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1154443.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3497..4276
                     /locus_tag="IW774_002109"
     CDS             3497..4276
                     /locus_tag="IW774_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1154444.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4263..5084
                     /locus_tag="IW774_002110"
     CDS             4263..5084
                     /locus_tag="IW774_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1154445.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5101..6150
                     /locus_tag="IW774_002111"
     CDS             5101..6150
                     /locus_tag="IW774_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1154446.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6163..7167
                     /locus_tag="IW774_002112"
     CDS             6163..7167
                     /locus_tag="IW774_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1154447.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2400 a         1233 c         1549 g         2084 t
ORIGIN      
        1 aggaacagcc ttttttcgcg caaccaaaag acttattctc ttttatgatc ggatacgacg
       61 ccagtttgaa agaaagtatc gaacaaatca aaacagctct ttactatcca gatggcggat
      121 taccactttt gattacaggc gaaagtggca caggtaaaag ttatttagtt aatttggtct
      181 atcagtattg tctgcttcat gatctgctag aagattctgc accattcatc acttttaatt
      241 gtgcacaata tgcagataat cctgaacttt tgactagcaa tttgtttgga catgttaagg
      301 gagcgtacac tggtgcagaa gaaaataaaa aaggggcttt tgaagcagca gatggtggga
      361 tcttgttttt agacgaggta catcgcttaa caccagaagg tcaggaaaaa ctgttcacct
      421 atcttgatca aggtgtgatt tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa
      481 cacgtctctt ttttgctacg actgaagaga ttactagtca tttcttaaca actttcattc
      541 gaagaatacc tatccgaatc gaactacctg cacttaatca gcgtagtcgt aatgaacggc
      601 tagaactgat ttactctttt tttctcgaag aacaaagaaa aatggggagc cctttgtcag
      661 tcagtggacc agctttgtca cttttaacaa gtcagaaatt atccggaaac ataggagagc
      721 tgaaaaatat cgtcaaagtt tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg
      781 aaatccatgt cacgatccat catttgcaaa aagaactact tgcgcagcct attactaaaa
      841 atacagcaca gcaaggaatc tatattacgc aagaccagac cttagagcaa ttaatggaga
      901 agcaacagcc cgaacagcag cgaatcgtta aaagttttga gcaaatccta attgatttca
      961 agaaaaataa ctgtctgcta tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat
     1021 tatttgattt tttgctgttt gagaccagta gacaagaaaa acacgagctg ctggtttatt
     1081 ggacacaatc tatccgagag acctttcgac aaatggaaac tgcctatcaa atcaaattcg
     1141 atggaaacag tgtctatgcc ttgagttact atctctatca gcgaagcact atcaaatggt
     1201 taccggaaga acatgatatt atccaactaa tcaaagaatt agaacaacaa gtttctcagt
     1261 cttatccatc tagttatcac tatgtccaac gaattctgga attaagtaag atcaaattag
     1321 atattgaaga cacaagtatg gatcggattt tactgacgat ctatctaaaa aaagcgaaat
     1381 ggtcaaaaga aaaacgactg ccaaaagcga tgattgctgc tcacggttat gcaactgcta
     1441 gcagtatggc aaatgtagta aatcgtttat tacaagagga tctatttgaa tcatttgata
     1501 tgccactaga tgtcacacca cagcagattg cttcagaaat ccttgattat tgcgaaaaca
     1561 gcgatgtttc aaatggattg atcatattag tagacatggg atctctgaaa gaaatccatc
     1621 agttttttaa aaaacagtta tccgtaccgc ttttgatttt gaataatgtg acgacacctt
     1681 tagcaatcac agtaggggaa tgcctgcaaa aaaatgtatc attagaagag atagcagaag
     1741 agactgttcg acagatccag ccagaatggc gactgctcta tccagaggaa aacaaaccca
     1801 aagcactcat tactacttgc ttcactggaa tcggtacggc ggtccactta agtgaactgc
     1861 tagaaaaaag cttacctacg gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat
     1921 taaaagataa gaaaaacagt gacccattat tttccatata cgaagtcgta ggaatgattg
     1981 gcaccacaga tcctgatatc accaatatcg actatctttc attagaagac ctcatctcgg
     2041 gggaaaagat gtctgtacta gctgaatggt tggacactac tatgaatgct tcagagaagg
     2101 aaatattcaa tcagcgcatc attcgtaatt tttcattgga aaaagtactg gattccgtga
     2161 ctattctaga tacagagaag gtgatgggag aaatcgatca ttttatgaga gaattagagg
     2221 tgcagttgaa tcgaagcatc agcaatccca aaaaattagc gttgtatgtc catgtgagct
     2281 gtctcattga acgattgatc cgacaaatgc caatcgaaac ctatcaagga ttagagaaat
     2341 ggaagcagtg tcaaaaagaa gggttatctg ctattaaatc tgcgtttagt gtcattgaag
     2401 agcattatag tgtcatgatc cccaattctg agattgccta tatctatgat attttgagtg
     2461 aagatacaga gtttctctcg atagaagaag aattttgaac ataagcaagt tgttgccact
     2521 aattttggca tgcaacttgc ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt
     2581 ggatagagaa attatcttag cttcccatgg aaaatttgct tcagggatat tagattcatt
     2641 agagctgatc tatgggaaaa atcattctat tactgtattc gattgctata ctgatgagaa
     2701 ttttgattta gcggaaacag taaggcagct ttttatgagg tacaaagata aagaaatcat
     2761 tgttctgacg gatctttttg gcggaagtgt caacaacgaa ttcttacaat atatcaatcg
     2821 ggatcatgtc tatctcgttg ccggattgaa tttgccacta ttgattgagc tagtatctcg
     2881 gttggacgct gaagtagaga caactgtttt gatccagcag gtgctaactg attcaaaaaa
     2941 aatgatccag ttttgtaacg agcggctcaa tcagaaaata gaagaagacg aattttagga
     3001 ggaagaaaaa tgattgtatt gacaagagtc gaccatcggt tattacacgg acaagtagct
     3061 ttttcatgga cacaaacaat aggcgcagac tgtattttga ttgctaatga tgatgtacct
     3121 acgaatgaga tacgaaagac aacgatcaaa ttagccaagc cacaaggagt caaactagtg
     3181 atcaaatcaa ttgatgactc gattgccgcg ttgaaaagcg gggtgacaga taaatataag
     3241 ttattcatcg tagtggaatc gatcgaggat gcatataagc tggcagaagc gtatccagaa
     3301 atcaaaacca tcaacttagg agggatcaag gcaaaacctg gtactcgcag catcggaaaa
     3361 gcggtaaata ttttaccaga agaggaggaa cgcttgaaac gattaattga caaacaaata
     3421 gaagtagaaa tccgtcaagt accagcagat aaaaaagtgg atgtaagaga agttttgtaa
     3481 gaaaggaaga acctcaatga ttgtacaagc agtcttatta ggaattgttg catttgtcgc
     3541 acaatcagaa tatgcactag ggacatcgtt gctttctcgt ccgattgtga cggggctgtt
     3601 tacaggaatc gtgttaggtg atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt
     3661 agcatttatc ggatcgttct cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg
     3721 gattttaggg acggcttttg ctattacagc tggagccggt acagaaaccg cgcttttact
     3781 aggtttgccg attgctacgt tgacattgat attgaaaaat atttacttag ggcttttgat
     3841 tccaattatg aaccataaag cagacaatta tgcagaagaa ggaaattata aaggagtcga
     3901 gcatatgcac ttgcttgccg gtatcggtct ttccttgatg cttggtgttg tggtaatggt
     3961 ttcttatatg gtgggaagca atacgatcgg aaatctatta aatatgattc ctgacttcgt
     4021 tcaaaagggg ttgtcagtag ctacagggtt gattccagct cttggttttg cgatgcttgc
     4081 tcgtttgctg atcaacaaac aagttgcacc atattttttc ttaggttttg ctatagcttc
     4141 ttacttagag attcctgtga ccggagtagc tatttttggt gcgattttag cagttgtggt
     4201 agtcaatatt atgaatgtcc gtcaactacc acttcaaaca actgcagggg aggtagaaga
     4261 agatgaagaa ttctgagatc aaaaacagca aaataacgaa aaaagagcta aatcacgtat
     4321 tttggcgttc ttttcaaatg gagttttctt ggaattatga acgtcagatg aatatggctt
     4381 atgtttatgc aatgattccg attttgaaaa agttatatca gtcgaaagaa gaaatggcag
     4441 cagctttgaa acggcatcta gagtttttta acacgacccc acatatcgtt acactgattt
     4501 taggaatcac ggctgcaatg gaggaagaaa ataaggatga tcctgaattt gatgtgactg
     4561 cgatcgatag tatcaaaaca tctttgatgg gacctcttgc agggttagga gattctttct
     4621 tttggggaac tttgcgattg attgccacag gtgtgggtac ctctttagcc atgcaaggaa
     4681 atattttagg ccctatcttg ttcattttga ttttcaatat tccgcacatt ctgtttcgct
     4741 atctagcaac aggttgggga tatcgcttag gaacgggttt tttgaaaaag atccaggcaa
     4801 acggtatgat ggaaagtttg acattaggcg cgtctattat tggactgatg gtcgttggag
     4861 caatgactgc cacgatgatc accatcaata ttcctttaaa aatcggttca ggagaaaatg
     4921 ctgtgactgt tcaaagtatt tttgatgata tcgttccaaa tatcttatgt cttggagcat
     4981 ttggcatagt gttttatcta ttgaagaaag aagtgaaacc attaacgatc ttgatcggac
     5041 tagctttctt tggaattttt ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa
     5101 atgtttacga tgcaagatta tatctatgaa gaaaaggaag tactaagtac gatcttgaaa
     5161 aagaatgatt tttcgacaag agaacatatg aaaaaaacag tgaatttact gatcttagca
     5221 acgggttcat cttataacgc ttgtttagca gcgaagccag cattggaatc ttatggtgat
     5281 cttactgtcg atattcaaga acctttttat tttttcaatt atggaaaact ttctccttca
     5341 atcgatactg tgattgctgt gtcacaaagc gggaaaagtg cttctactgt tgaagcagta
     5401 aaaatgatac aaaaacaagg gttacctgtt gttgctatca caaatgacgt gcaaagtcca
     5461 cttgctttag aagctgcaca aattattgat ttaggtgccg gggtagaatc agtaggcttc
     5521 gttacaaaag gctattctgc gactgtttta cagttactac tcttagggat aggaattgga
     5581 ataagtaaaa ataaaatcag caaaaaaata gaacaagact acatgcaaca gttgcgcaaa
     5641 ataatcaacc atttaccagc gattattcaa aaaacggaag aattttttga tggatatcaa
     5701 agtttatttc gtttagcaca gcgtttcgtc accattggat atggtccaaa ctggggaaca
     5761 gctaaagaag cagagacgaa attgactgaa acaatccgcg ttccgtcaca aggatttgag
     5821 ttagaagctt atatgcacgg cccatacctg gaggcagatg cttcgcattt gctattcttt
     5881 attgaagggg aaagtgtaaa taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg
     5941 tacgttggag agactctcac cattaccaca aaaaaagcaa gaaacgagaa aacacttgga
     6001 ttagcaatag aatgtgatga atatctgtct gtattagcgc ttgtcgttcc gtttcaacta
     6061 ttcgcttata aaaccgcagt agctaaaggt attgacttga acaaaaagat ttttgaagac
     6121 tttgatacag tcttgaaaag taaattataa gaggtggaaa tcatgctgaa attcaatgaa
     6181 gaagaacaaa tcaaagcaat aaaaggcgca ctagctttac gtccgcaagt agaagaaatc
     6241 atcgataaac tttatgcgga aaaatttgac gctgtctatt atctaggaat cggtggaacg
     6301 tatgcttcct cgatgcaagc tgtcacttat atgaatggga aaagcaactt gcctgtcttt
     6361 gtacagcatg ctgcagaata ttacactact ggaaacaaaa gattaacaaa agattctatc
     6421 gttattttgt cctctgttac cggtacaact caagaagtag tgaaagcagt agaacaaatc
     6481 aagaaagtag gagcgacact gattggattt atcgataaag cgaacagtaa gttgtctcag
     6541 ctatgcgatt ttgtggtcac ttatccagct cctggaacag aacaaatcaa attttttatg
     6601 gcagcagatc gattgatgta tttgaatgga gaattcgaag cttattcaga atactatgaa
     6661 cagctcgaac gttatttacc aacagggtta gtggaagcag agaaaaaagc agatgcattt
     6721 ggcttatcat ttgcagaaaa acatcgacgt gatagtatgc attattttat tggagcaggg
     6781 aatcaatggg gagctgttta ttcatatgcc atgtgttact gggaagaaca aagctggctt
     6841 ccatcgaaat ctatccatgc tgccgagttt cttcacggga cgttagagat cgtagaagaa
     6901 acaaccccag tcacattatt tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg
     6961 gctaaacttt tgccaaaaat ttgcagcaat tatacattga tagacacaaa agactatcca
     7021 gtagaaggta tcagtgaaaa atatcgcgga cgtgttttgt ctttcttatt gatgcatgta
     7081 gtgacacaac gcatcgatgc tcatgtggag cagttgaact gccatccatt agagatacgc
     7141 cgctactatc gtcagtttga ttattaggat agactggata aaaagaatat aaaaaataaa
     7201 gagagcagcg ataactgctc cctttatttt tttaaaatat tggcatcaat tacgttataa
     7261 aacaca
//
LOCUS       DACEOE010000128         7237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEOE010000128 DACEOE010000000
VERSION     DACEOE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.130"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(6..1208)
                     /locus_tag="IW774_002113"
     CDS             complement(6..1208)
                     /locus_tag="IW774_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1154448.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1325..1840
                     /locus_tag="IW774_002114"
     CDS             1325..1840
                     /locus_tag="IW774_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1154449.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2039..2485)
                     /locus_tag="IW774_002115"
     CDS             complement(2039..2485)
                     /locus_tag="IW774_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1154450.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2532..4010)
                     /gene="msr"
                     /locus_tag="IW774_002116"
     CDS             complement(2532..4010)
                     /gene="msr"
                     /locus_tag="IW774_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1154451.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4364..4993)
                     /locus_tag="IW774_002117"
     CDS             complement(4364..4993)
                     /locus_tag="IW774_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1154452.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(4977..5441)
                     /locus_tag="IW774_002118"
     CDS             complement(4977..5441)
                     /locus_tag="IW774_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1154453.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5928..7196)
                     /locus_tag="IW774_002119"
     CDS             complement(5928..7196)
                     /locus_tag="IW774_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1154454.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGFTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
BASE COUNT         2200 a         1507 c         1248 g         2282 t
ORIGIN      
        1 atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat acaacataca
       61 ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat atgttcacca
      121 atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa actatagctg
      181 gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt caacagtgta
      241 ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag gactggcata
      301 gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc tgctaaaatt
      361 gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga agcaatagcc
      421 agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata gccaatcaat
      481 aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa tgcgattagt
      541 ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag gcctactttt
      601 ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc gcaaaacatc
      661 aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac cccaccgact
      721 agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat tgctttttct
      781 gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa taaagcattc
      841 ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga taatccgcca
      901 agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt caaaccgatc
      961 cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat gtaactagta
     1021 aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt ttttaagatt
     1081 gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac aactagcgct
     1141 gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg attctccaat
     1201 atttccatcc ctttctctct attagcatca tacatgattt attttacatg taaatatgca
     1261 catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga gaggtaggat
     1321 ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac cacgttctgg
     1381 gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc agatctatac
     1441 aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag acgagcactc
     1501 tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg aatggatcaa
     1561 agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct atgctttttc
     1621 gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac agctgaacaa
     1681 aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga aacaagaatt
     1741 tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac tccaatggtg
     1801 tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca gaacctttct
     1861 agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt tggaaaagaa
     1921 aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt ctagaactgc
     1981 attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca gtgttccatc
     2041 attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg atgatcaact
     2101 gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca gagaataatt
     2161 gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc accattccta
     2221 aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga tttactggta
     2281 gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa atgaaagcca
     2341 ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct tgatgcagcc
     2401 ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt gtaaaaatta
     2461 ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc acgaaaatca
     2521 tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc ttttatttca
     2581 taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa caaaatcgtt
     2641 cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc aataaaatta
     2701 gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc aatcgtcaga
     2761 cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc ctgagcaaac
     2821 cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg tctcatgaca
     2881 aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata tacttgaaag
     2941 cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc taataaggtt
     3001 gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga aaactgaaaa
     3061 tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg tcccatgatg
     3121 ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg tttcatctgc
     3181 tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt cgcattcttt
     3241 tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc cggtctgatt
     3301 tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt tttcttttct
     3361 tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc tcgtagctga
     3421 gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga aaactctcgg
     3481 atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa atagcgatca
     3541 tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag tcgatcaata
     3601 ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc ataagaagaa
     3661 agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg ctctgaagtt
     3721 tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt tgcttcagcg
     3781 agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt cccatgatcg
     3841 ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc attttctcct
     3901 atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag ttctagctgg
     3961 ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat atgttttaca
     4021 ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa aatctgtttg
     4081 caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac taaatcacca
     4141 aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat actccatcca
     4201 tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa aagttcgaaa
     4261 cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt accacacacg
     4321 attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg aacgtagtga
     4381 tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt cctattgata
     4441 aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc aactgatctg
     4501 cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact aacatgaatt
     4561 gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct cttgaaaaac
     4621 tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt ggcatcttcc
     4681 ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc atatcgccaa
     4741 aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg atcaaaggac
     4801 gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt tgcatattga
     4861 cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg tgtgggattt
     4921 tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt gaaaaatcat
     4981 ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca aagttgggta
     5041 ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa agccggggat
     5101 agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt ctgggctttt
     5161 tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg cgaaacatag
     5221 ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat ctgcaacttt
     5281 tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat gatctcgtat
     5341 ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg atcggctaat
     5401 ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc ctttctaata
     5461 attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa agtcaaagaa
     5521 tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag aaattttttg
     5581 tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat tattgattct
     5641 tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc aaaaatttcc
     5701 gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta tgcttaaaca
     5761 gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac ggtgttcaaa
     5821 aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct gtttttgttt
     5881 ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta ataaccataa
     5941 aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc cttagcactc
     6001 aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata aattggcttg
     6061 gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt taaagagttg
     6121 atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta taaacggtag
     6181 acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca gcctacagtt
     6241 ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt atgacggtta
     6301 tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt cagattatag
     6361 gtttcgatta cattgttcaa tgtaaaacca taattaggcg cagtggcata acgacctgtt
     6421 agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc ggaatagtag
     6481 taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg caatgattct
     6541 ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc attcacatat
     6601 tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc aaatagattg
     6661 taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat ggcttgagca
     6721 atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt tgaggcaata
     6781 ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc atagccagta
     6841 tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg cccatctgtc
     6901 aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc tgatgtactg
     6961 tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga aattgtattt
     7021 tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc tgtttctgaa
     7081 gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac tgttgtgttg
     7141 gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc tttcatcttt
     7201 tttctccttt gaattatgaa acttttccta aataaca
//
LOCUS       DACEOE010000129         7220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEOE010000129 DACEOE010000000
VERSION     DACEOE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.131"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            10..285
                     /locus_tag="IW774_002120"
     CDS             10..285
                     /locus_tag="IW774_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HAQ1154455.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            394..2220
                     /locus_tag="IW774_002121"
     CDS             394..2220
                     /locus_tag="IW774_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154456.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSIGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELVVSSEETEEVEEIETVSENEEETEEIFDFEDEEEVENVQKGDLS
                     TSSQNGLEAVNDPQVPPYISSQTPNYSPQYQAPNVLQPLRKHETVQLVTLKEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDIAWKKPYDLDVKKNKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            2233..2949
                     /locus_tag="IW774_002122"
     CDS             2233..2949
                     /locus_tag="IW774_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154457.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKG
                     HYENINAQISCPLDRNGETTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     TKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEIKSFDKRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            2962..5820
                     /locus_tag="IW774_002123"
     CDS             2962..5820
                     /locus_tag="IW774_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1154458.1"
                     /translation="MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFD
                     PKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVI
                     QSYSQLETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLD
                     TDSSVTSSAEQERVITVERASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMP
                     YRYQFLAEDFDTQRDINEIDIACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPI
                     SEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLSEQEMKINIYLKESQKE
                     AIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQIMIL
                     EQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI"
     gene            5871..>7220
                     /locus_tag="IW774_002124"
     CDS             5871..>7220
                     /locus_tag="IW774_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154459.1"
                     /translation="MQWLKYKWPIIVSLIIILFMAYPQRVFALPWITDNKEVLKFLTK
                     YQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFSFVESSSLGAVY
                     QSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALILLMPTMISSGIQ
                     FAKTFYKDSTTITKADGGVAWSLIEDGVTDLVYINKNDAYDRLGKDGVQKNALKEKNF
                     FSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDGWMSMFSDSFKG
                     GYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLVFATDIETGQRS
                     KVVLSDILQCYLTVGFQGFGLSMFAMFVSYLGTGGGSSVNPFIKAVAYVCAVFVLIKG
                     SGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGNGNGSDSGEGED
                     "
BASE COUNT         2707 a          931 c         1440 g         2142 t
ORIGIN      
        1 ggaaataaaa tgaaaaattt agttatcagc aagtttgcaa tgttaccaag aatggatttt
       61 ttcgataatg taagtaagtt tatgtcaaat atgacttcaa aactacaagg aatttcacta
      121 tctgtaatta tcttttgtat tgtggttact ggtttgatgt ttcttttcgg agaagggcca
      181 agtcgtactg ctaaaaaatg gttaacttat atcgtgattg gggcaataat tatcttcgga
      241 gcttcaacta tcggacaaac gatcaagtcc gttggtggtt tctaagggga agcttcttag
      301 aactaaaaaa ttcgcaagta ttgcatgaaa gagcattgag tgattaatgc tcttttttat
      361 tttgaaaaac tataaactaa ggagatttaa actatgaacc aacgattcga atataatttt
      421 gaagatgtag aacctaagtt actgaaaaaa ggaaaaggat taaaaaagaa aaataaatat
      481 actctcaaac aagcagaagc attagtagga tggattcaac acaattggtc agacaagaca
      541 acaattacct tgatctgttc agacacagaa gaaggaacaa agtatgcttt tgatggaatt
      601 gaaattactc cattttcgat tggcggacaa gaaaaattat tgtttcctga tattgaagga
      661 caaattttac aagcgccgga cagcatgatt tccgatccta ataaactgat tttggatatt
      721 aacgatcaat acaaacacga accagacgta gcagaaccaa caaaagaatc aaagtcaaaa
      781 ttaaatttga agctcccttc agtagtatta ccatttgcta agaagaaaaa taaacttatg
      841 aacgaattga ttgaaccaga agaaacggat atttatgaag aagaattaga agaacaagaa
      901 gagatcgaag aattagttgt gtcttctgaa gaaacagaag aagtagaaga aattgaaact
      961 gtttctgaaa atgaagaaga aacagaagaa atctttgact ttgaagatga agaagaagtg
     1021 gaaaatgtac agaaaggcga tctatctaca agtagtcaaa atgggttgga agctgtaaat
     1081 gatccacaag ttccaccata tatatcttca caaactccca attactctcc acagtatcaa
     1141 gcgcctaatg tcttacaacc attaagaaaa cacgaaacag tgcagttagt tacgcttaaa
     1201 gagtactgtg atttgggaga agaaattgaa acttcaattt ctcaaatggt cgaaaagttg
     1261 aaaccagaat ctttattcaa attcataggg attccttcta caggtcttac aaatactcgt
     1321 attgatgaat atagaagtaa ccatgctatg ttgcgattag ctgaagtaaa atttgaaaat
     1381 ttaagagagc attattcacg gcaaattatt tcttttaaag cagaagccct agatcgttta
     1441 agaactgcgg tagatattgc ttggaagaag ccttacgatt tagatgtgaa gaaaaacaag
     1501 gccgaagagt tatcaaaaat ggaagcagaa gcagaagcta aattaaatga gttcgtgaaa
     1561 cgtcaggaaa acaatgtaaa tgataaaatc aaaaagtttg agatcgaaca agaaatagat
     1621 ttaaaaaact ttattgcaag acaagaggca gataaagcgg tatttattgc tacagagaaa
     1681 gatcggactc aaaatttaat tgattctaaa gcggaatcaa tcaatgcaga tttacttgaa
     1741 acaaaagata aatttattga tgaagaaatg tacaaaatga aaattgaaat gaacaaaaat
     1801 ttatttgatg gcaagcaaaa aattaaatca gaattggcta ctgaactcgc taaggcaaat
     1861 gataatgttt gggaaaatgc gctgaaattt attcaagaaa ttcaagatga aattgaagct
     1921 aaaacaccaa tttgggctgc tgaaatcaag gagtttaata gtttagaaga acaagaacat
     1981 gaacgtatga agcaaactaa agaacttcaa ttaaaagagg aaagtattga aatcaagcgt
     2041 ttggaagctg aaatgaattt gaagcgtatg gaagaattag atcatgaaaa taaaatgctt
     2101 aaaattaaac ttgaaactgc cctttcaaaa aatgagttat tagctatgga acaagataca
     2161 aagcaagtgg tgaatggatc aaacagagtt ggtgcactta atttatttag taaacgttaa
     2221 aggagaacgg atatgagtgt ttttaataat aaagttatgt tagggaactg ttatgtgaac
     2281 cacattggtt tttacgatgg aggaacatac agagttttat tcttacgttt atctgttttt
     2341 agttactcaa aagatggaaa ggggcattat gaaaatatta atgcacaaat ttcttgtcct
     2401 ttagatagaa atggggaaac cacgactaca agtagtcacc ctaaagcctt gatgaataaa
     2461 tttatctttg aaaagttgga tcgcaatttg gttgagggga tgaagataag tattcatgga
     2521 aatattcgtg gaacagaaca agtgcttctt aacagtggtt ggaaaaactt tggtcaatta
     2581 tcgcctttag aaaaagaaga atatcaaaaa attcctaaaa atgaattagt aacaagaaat
     2641 caaacatata tctatataga agaggtaagt atgccagaaa aagaagaatt tagaaagtct
     2701 caatctaagg taacaaaaga agatgtggaa gttgcagaac cgaatcaagc agatgaaatt
     2761 ttatccgatg atactgatcc agtgaatgat gaaggagagg ttattgaagt ttttgaagct
     2821 gaaattatcg aagaggaaga agaaattaaa agttttgata aaagaatgta tcgagagaaa
     2881 ccaaaaggtt atgtgaattt tgaagatatg tattatgacg aatattctaa tgaaccaccg
     2941 atcaattagt gggggtcaaa tatgaagcta gtacaaaaca atgaagtgaa agaaataaaa
     3001 aacggctttg ttaaaaactc gaacaaaatt ttgggaaagc tgaaaaaagt aggtagtttc
     3061 aaaaaaaata aatatcatac tggaaaaatt gagggagaag atttaccaaa aagtttacaa
     3121 gataaattga atgttccgct tgtatttata ggtgtttttt taggttcaat agtttttcta
     3181 gcaataacgt acttattgaa ctttttaaaa gctttgttag ttgcaatcgg acagtcagaa
     3241 agtgggctac ttggtgtaga gttcgatcct aagtttagtt tggcatatct aaaaccactt
     3301 ggggatacga aacaatactt tatgtgtgct gtggttgcgg taataggagt cgcttattta
     3361 atatatggga aattaaatca tgaaagtgag aaaaatcttg tttacggtca aaaaggagat
     3421 tcaagacttt caactatcaa agaaattaag agagattacc atgagatacc agaaaaaaca
     3481 gaagtgtatg atggtattgg tggtgtgcca attagtcact atcaagataa atattatatt
     3541 gatagagaca cagtaaatac cgttgttttg ggagcttcac gatccggtaa aggggaaaca
     3601 tttgttgttc ctttattaga taatcttagc cgagcaaaaa tacaaagtaa tatggtcgtg
     3661 aatgatccga aaggagaatt gtttagcgct tcaaaggaaa cacttgaaaa acgaggttat
     3721 cgtgtattag tgctaaatat tgatgatcca cttgaatcaa tgagttttaa tcctttacaa
     3781 ctagtaattg atagttgggc taacaaggac tatcacgaag ctagtaagcg agcgaatact
     3841 ttaacttcaa tgctctttgc ttcaggaatg ggtacagata atgagttttt ctataaatca
     3901 gctaaatcag cggtcaatgc gattatttta acgattgtag aacactgttt taacaatgat
     3961 tgcattgaaa aaattacaat gtacaacgtg gctcaaatgc tgaacgaatt aggaagctta
     4021 ttttacactg atccaaagac tggaaaagaa aaaaatgctt tagatgaata tttcaatact
     4081 ttaccacaag ggaatcaggc caagattcag tatggttcta cttcatttgc cggtgataaa
     4141 gccaaaggtt cgattttatc tactgcttca caaggtattg aaatgtttac aagtgatttg
     4201 ttcgggaagt taacaagtaa aatgtctatt gatttgaaag agataggctt tcctaagtcc
     4261 atacaattta aagtcaatac gaatttagtt ggaaagagga ttagtatttc atttttgaga
     4321 aaagaaaatg atgtaattag attaattaag aagtatcgag tgaaagtaaa agctttaggg
     4381 ctatgtgttt taaattttaa tgaatatctt caggatggag atatgttgga tatacgatat
     4441 gaagaagaga aatcgagagc atggtatcgt attgaatttc ccaaaaaaca agcaaatagt
     4501 catgatactt ctttagtcac taaaaaattc aagtcaggta aaaattattc tgacttagaa
     4561 atcagcacct tacgattaaa atacacggat cagccaactg ctgtgttcat ggttattcca
     4621 gattatgatc cgtcaaatca cgtacttgca agtattttta tcagtcaact ttatacagaa
     4681 ttagcaagta attgtaagca aacaccggat aaaaaatgtt ttaggcgagt tcatttcttg
     4741 ttagatgaat ttgggaatat gccggctata gacaatatgg atgggattat gacggtatgt
     4801 ctaggaagaa atatgctttt tgacttagtg attcaatcct atagtcagct agaaacaaga
     4861 tatgataaag cttttaaaac aattaaagaa aattgtcaga atcatatttt aatcatgtcg
     4921 aacgatgagg aaacgataga agagatttca aaaaaatgtg gtcataagac aacaataaat
     4981 agaagtgcta gttctaaaca tttagatact gatagctcgg tcactagttc ggcagagcaa
     5041 gagcgagtaa ttacagttga gcgtgctagt caattgattg aaggggaaca aattatttta
     5101 agaaaccttc atagacaaga tacaaaaaga aataagatta gaccatttcc tatttttaat
     5161 actaaagaaa caaatatgcc gtatagatac caatttttag cagaagactt tgatactcaa
     5221 agagatatta atgagattga tattgcatgt gaacatatca atctatcttt acaagataac
     5281 caaattccat atgcaaggtt tatcaaagat ttaaaaacac gtttggagta ttcaattatt
     5341 aataatattc ctattagtga agaagactat caggattatc taaatttatc aggatcacaa
     5401 caaacaatta atgaagaaga gctgttaaaa ctggtaaata cggaagtaaa aagtcaagat
     5461 atggaaggtc aagttttatc agaacaagaa atgaaaatta atatttatct aaaggaaagt
     5521 caaaaggaag caatagaatt agtaaaagta attaatggaa ggttagctga tgaaaattca
     5581 gcagatatgg ttataaaaac cattaattca caaatccttg atacgccttt cattcaatct
     5641 ttaatcaatg attctatagc tattcataat gttcaagaga gtaaggatca aattatgatc
     5701 ctagaacaac aattagctaa tgcttatgag ttagcagtgt ttaaaacgaa aaattcaata
     5761 ctgaaaaata aaattatcaa gttgaaaaat gctatgacta agctagcaaa agcaatttag
     5821 agagaaggga agaaatattg actgaatcta ttaattggag aaaactaaat gtgcaatggt
     5881 taaagtataa atggccaatc atagtttccc taataatcat tctatttatg gcctatccgc
     5941 aacgagtttt tgcgttgccg tggattacag acaataaaga ggtattaaaa tttttaacaa
     6001 aatatcaaga ttatcttcag tttactaaag gtggttttgg aagcattgtc gttcaaagtt
     6061 tatcgtgggg gctgattaga ggtgcatatt ttgtttgtaa atcagcagaa aaccttgttc
     6121 cacaatcgct aagtttgttt agctttgttg aaagcagtag tttaggtgct gtttatcaat
     6181 ctgtattaaa cacggtggtt gtaggattaa tgatcctttc attgatgttt gttggttaca
     6241 agatgattac tggaaaagga acagtggatt taaaatcggt tggaatgaac gtagtgatgt
     6301 ctatagcatt aatactttta atgccaacca tgatttctag tggtattcaa tttgctaaaa
     6361 cattttataa ggactctact acgattacaa aagctgatgg gggcgttgct tggtcattga
     6421 ttgaggacgg tgtaactgat ttagtatata tcaataaaaa cgatgcttat gatcggttgg
     6481 gtaaagatgg cgtacaaaaa aatgcgttaa aagaaaagaa tttcttttcc tacgatttca
     6541 gtgaagtaat cacagttgat acagctaaag atttagaaga tgataataaa aaagctgaat
     6601 atcttcaatc taggttgaca ccagatggcg aaggaaatat gcaagccgtt aagattgatg
     6661 atggttggat gtcgatgttc tctgatagtt tcaaaggagg gtactacagg tttactaaaa
     6721 atacttttgc attgacttta ggattacttt cgttagctgt agcctatgtt tttagtattt
     6781 ttatcatagt caccgctatt ttagaactgg catttaagcg tgtgttaggg gttcttgttt
     6841 ttgcaacaga tattgagaca ggacagcgtt caaaagttgt tctatcagat attctacagt
     6901 gctatttgac tgtaggcttt caaggatttg gattatccat gtttgcaatg tttgtttcct
     6961 atttaggaac aggcggaggg tcttccgtta atccgtttat taaggctgta gcttatgtat
     7021 gtgccgtttt tgttttaatc aaaggcagtg gaacagtcat gagatacttt ggtgttgata
     7081 ttggactcaa agaaggctat gggcaacttg cttctgcttt tggaatgggt gcaatgctgt
     7141 ttagaaaagg atcaaatgga tttaatcgtg ccaaaggaag cggaaacgga aatggatcag
     7201 acagcggaga aggagaagat
//
LOCUS       DACEOE010000130         7216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEOE010000130 DACEOE010000000
VERSION     DACEOE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.132"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..484
                     /locus_tag="IW774_002125"
     CDS             <1..484
                     /locus_tag="IW774_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1154460.1"
                     /translation="LSKDDKIVMGKRIREVRKNREETLEKFGKNFTIYTGKNVISRWE
                     KGINIPDIEKLMNIAYLGKVTVPYLMYGEDYKNILPKDERVSDFNKINSFSMGLRMRK
                     IRKDYYLEREEFGKLFSPSISKWSIDRYENGRDIPNTNRIIQYAYIGNLSLEFLIYGI
                     "
     gene            1482..1733
                     /locus_tag="IW774_002126"
     CDS             1482..1733
                     /locus_tag="IW774_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154461.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFVESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            1816..2088
                     /locus_tag="IW774_002127"
     CDS             1816..2088
                     /locus_tag="IW774_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154462.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            2178..2417
                     /locus_tag="IW774_002128"
     CDS             2178..2417
                     /locus_tag="IW774_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154463.1"
                     /translation="MIDISIPEDLKPDPATIGNSGMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMIFGDGEVANKAGIPIGKWTVDFHEAGSFFVL"
     gene            2564..6859
                     /locus_tag="IW774_002129"
     CDS             2564..6859
                     /locus_tag="IW774_002129"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ1154464.1"
                     /translation="MYAKTKMNWLKALALVALSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKLNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KNMNGKVQAEIKDLDPGKYVLKEIKAPKGYALSDETIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKIANKDLVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKERYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVT
                     FTLYNGKGQAIGKYTTDQNGEILVKGLVANNYYFTENVPLESFQPNTGKQAFTITFRQ
                     NAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQEGKEITSILTFIADKANGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAVHVDINDDNQSVRVNNPTVHTTAVFEDGLTIADPTGLFTLNDTVEINE
                     ILPGKAYLVKGILMDKATGEALLVHGQPVESAVGFIAKAENETVIVPFTFDLTGLNGR
                     EMVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLIVGKTYTVNGVLMDKVTEKPVLVDGKEVSSTLTFVAETTDGTVNLDFILD
                     AHALRSKEVVVFEDLFREGVLLASHADITDKDQTIKIVSPTITTKATNANGGKEILAL
                     PNQKVVEWVQVNGLAIGQIYKLIVQGYLTPDGTPFEGTHAEKVFMADKEMMEFLFEFI
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQFVEAPKPPQPVK
                     PNEPQPVKEVKQAAMVPMLPKTGSEDNVLWIMIGMIFVLAAGLIGYDLYKVRD"
BASE COUNT         2566 a         1119 c         1530 g         2001 t
ORIGIN      
        1 tttatcaaaa gacgataaaa ttgttatggg aaaaagaata cgagaagtcc gaaagaaccg
       61 ggaagagacg cttgaaaaat ttggtaaaaa ctttaccatc tatacaggca aaaatgttat
      121 aagtagatgg gaaaaaggta ttaatattcc agatattgaa aaactaatga atattgccta
      181 tcttggtaag gtcactgttc cttaccttat gtacggagaa gattataaga atatacttcc
      241 aaaagatgaa agagttagcg attttaacaa gataaattct tttagtatgg gattaaggat
      301 gaggaaaatt aggaaagatt attatctaga aagggaggag tttggaaagc ttttttctcc
      361 ttcaatttca aaatggtcca tagataggta tgagaatggt agagatatac ccaatactaa
      421 tcgaattatc caatatgctt atataggaaa tctttcttta gaatttttga tttacgggat
      481 ataaaaatag tttacgtcga tacttattaa gttatcgacg tttttttatt atagaaaaat
      541 ttataaaagg aggtttcttg atatgataag aaacaaaaag ggtttgttga aagttgcttt
      601 actggcagct tttttctaag taactttttg acaagctcaa tggcaacaca agcggaagaa
      661 gtaatacaag aaataactgt tatgaatacc agctagataa aatgggctag gtatgttatc
      721 caacaattta gtaatcacta gaagcctagc gaaagttcgc ttgataagtt atagatgagt
      781 attgatttgt agtctttcca aatcaaaagg gtacagagcc acatgaaata tgttgtcctc
      841 tgcagcgtca ggatgggaat ccataccatg gaaattcgga gatctgtgag ggaatactta
      901 cgtggttaat atgtcggttg tgttggacgt agtaatggtc atagtgggta gattgactga
      961 attcttatat gagaaaatga aaccaacaag aagatagggt tatttataca gaattgggga
     1021 tcttttctag aaaagtaatc ggacaatgcg agattcgtac ggtctttttt aaatttctct
     1081 taaaaaaacg gcatcgaatg ggttgggtga ttgataagca atgaatcata gactgatata
     1141 gaataaaaaa atgattctat ttatcttttt acatctatcg ttttttcaag taaccgcaaa
     1201 ttgtatcagc attcaacaat ttttgtatac aagttctagt aaaaggatct gaagaaaaag
     1261 ttggttgttg tgaagataaa aaaagaatca ggttttactg agtttttctt caaaatttgt
     1321 gttattaggt taggacaaag ataagaagaa gtatgaagat ttaaaaaagg aatgaggaaa
     1381 agatagtaat ttccgattat tggagttttt ttattttata aagaagaagc gaacaagttg
     1441 tgattcctag atttcgcgcg tgattaatta atgagcaaaa aatgcggaaa gttttagtgt
     1501 ttaatacacg aggacaaagt ttcttaggta ttgaaggcac acgtgtaacg gttgatgggt
     1561 ggtgtgattg tttcgaggtt ggaaaacatg ttgaacttat ggcatttact gagctaaaag
     1621 ataagaacaa cgtggaattt gtcgagagtg atattggttg ggacgatcat gaagaagttt
     1681 atggaaaatt tattttgcgg aagggaaatt cttatacgaa tggggaagca taagtagcga
     1741 tttgttagag atcattgata ggattgaaat tgtcgggaat atttatgaga atctagagtt
     1801 atttgaggtg atagaatgag tgatggtttt gaacaagtat caataattaa aaatcaagtt
     1861 cgggagattc ttagaaaaaa aggctatttg gtagatagtt attttgaagg agattacgaa
     1921 acatgggtag gtgtctatgc tcgtccagag aataaaccta cctatttaga tcctacgacg
     1981 tcagaagatg cctatttaca aaataggtat cgagtagatg gttttaagca agactttgcg
     2041 gagtggtttg aatgggaaat tgaaaacgga gaagtaaaag aagaataagc gtacttgttg
     2101 tatcaaaata agtcagtaga acaaccaaac cagtagctag taatgacaat aatactgggg
     2161 aatcggatca ttatggtgtg attgatattt ctattccaga agatcttaag ccagatcctg
     2221 caacaattgg caacagtggc atgatttttg attcgttaga tgaagctatg tcatatggtt
     2281 ggaaagaaat taaggatgaa ggtagtaatt ggtatggcat gatatttggt gatggtgaag
     2341 ttgccaataa agctggtatt ccaattggga aatggacagt tgatttccac gaagcaggtt
     2401 ctttttttgt tctgtaatta tagtgggagg gtaaaatcat tgatgaaatg tgttcagata
     2461 agagcgccag ataaaaatag aagagacagt tcacgtcgtt atcacttttg gtaacggtgt
     2521 tttgttttat tatataaaat tctcaggaaa aggaggaatt cttgtgtatg caaaaacgaa
     2581 aatgaattgg ttgaaagcac tcgcattggt ggccttatct caattctgtt tcttggtggg
     2641 aactgctcaa gcacatgcag aagaaacgac ggctacacaa cctacggtgg aagccattgc
     2701 ccaagtcgct tctgaagtga cagaagtcca accagcagaa gccggggtcc cagctggtga
     2761 agccactgtt gaacaggccc ctgtgacaga agcgccaacg gttaattcca aaacgactct
     2821 cgatcagacg attggggaaa cacaacaccc agggaatagt cctgttactt cacaaccagt
     2881 tgaagaaact gcctcaatta caacccctgc ttcagaaacg tccactccag aaggagaaaa
     2941 agctgccgaa gcaacgcctt ctgtgaagcc agagtcttct gagaaacaag cggaagaaaa
     3001 gcaacgaata gaagaaacag tggaacaacc agcggctact gctgacaaag aaggaaaaaa
     3061 tgaggaaaac gttgaaaaaa caatctcttc aaccgcagct agtcaaaatc aaccaacgaa
     3121 actaaacaaa gaatctacgg ctagtattga agatcaaaag aaaaaagagc aggacattcc
     3181 aaaaacaggt acacctacac tgttagtaac agtaccaacc aatcgttcgg ttcgtgttcg
     3241 ccgtgcaacc aatacagctg ttttaggtgg tcggaaaatt gattggggcg aaaatattgg
     3301 tgttacttct agtccgaaca atccaaactt cactttaaat tatatggttc gttttgagat
     3361 taatggacaa gtggcgtttt gtattgagcc tggtgtctca gccgcagaag gtcaagttta
     3421 tacagctact gctttggaaa actatttgaa aaattcaaat gtacggaaaa aaacaagctt
     3481 gatctcttat ttaggatatg tcacaaataa agacaaatcc gatgaacaat acattgcggc
     3541 acaatttatg atttgggaag ctttgggcac aaaaatccca atcaaaaata tccgtgttga
     3601 ttatgatcgt cgaaaagcag aaattcaacg tctgcttaat aattttgatg taagagctac
     3661 ttttcataat caaacacata cgataaaaat tggcgaaacg ttaaaagtaa aaagtggcaa
     3721 tgactttcta caacgtgtaa aaggcattca aaagccaaaa gggatcaatg tatctattca
     3781 tgggaatgaa gcatggatca cagtcacaaa agaagcacca gaaaaggcaa aaattcattt
     3841 caatcagatt gaagtattgg gtacgccatt agcgtatcgt cgtccaggtg cccaaacaat
     3901 tggtatattg aatccttttg aacctggtga ctctgttgtc aatttgacta ttttgaaaca
     3961 gggaaacatt cgtgtcttta aagaagatgc agaaaccggt gtgaaagcac aaggcgcagc
     4021 ttctttagaa aatgcggtgt atggcttgta tcaagcagat gggaaaaaga tcaaagaaat
     4081 taccttgaaa aatatgaacg gcaaagtaca ggcagaaatt aaagatcttg atcctggcaa
     4141 atatgtttta aaagaaatca aagcgccgaa aggctatgca ctttctgatg aaacaattga
     4201 aattgttgtt gaaccaggaa aaactgtcaa tgcgaccgca aaagatcaag tgatcaaagg
     4261 aaccattgat ttagtgaaaa ttgctaataa agatttagtc gattcaacca atccagataa
     4321 caaaccaaaa ttagctggca ttgaaatctc tttgacgtca aaaacaactg gcaaagtggt
     4381 gaaaaccgtt gtgacagaca atgatggtta cgctactttt ggtaaaaata ctgttgtctt
     4441 tgatacctac attctttctg aaaccaaagg aaaagaaggg tataaactat ttgaaccact
     4501 tgaagtcaca atttctgaaa atgggcaaac tttccattat gttttagaag ataaagtaat
     4561 tgaacaacgt cttaaagtgg tgaaagtgga tcaagaaaca ggaaaagtga ttccacttgc
     4621 caatacagaa ttcaagattt ttgatacttt agccaatcgt tatgtcacaa tggagattcc
     4681 aaatgataca gaaacaacgg atatctttaa aaccaatgaa aaaggctttt tcctaactaa
     4741 cggtacactt acttatggaa aagagcgcta tcgtttggaa gaaatcaaag cacccgtaaa
     4801 ttacgttttg aatcaaaacc cattagtgtt ctcgattacc aatgatacag aaccgatcaa
     4861 agtgatccat tttgcgaacc gtcaagcacg gaaaagtgta aaattgttca agtatgaaga
     4921 atggaacaag aagaaaacac cacttgccgg tgtgacgttt acgctatata acggaaaagg
     4981 tcaagcaatt ggtaaatata ccaccgatca aaatggtgag attttagtaa aaggtctggt
     5041 tgccaataac tattacttta ccgaaaatgt accattagaa agtttccaac caaatacagg
     5101 caaacaagca ttcactatta cattcagaca aaatgcgcca gatgatgggg aaacgttatt
     5161 ggtttctgta cgtaactatt tgattccacc aaaattagaa acacaagcgt caaataaagc
     5221 cgatggtgaa aaagtggttg atccattaga aactatcgtc attaaagata gtgtttctta
     5281 taccaacctg tttgtcggca aagaatacac tatttcaggt gtattgatga acaaggcaac
     5341 gaatgcgcca attttacaag aaggcaaaga aatcacaagt atcttgacct ttattgcaga
     5401 caaagccaat ggcacaaaag atcttgaatt tgtggttaat gctagtgttc tgaaaggtca
     5461 aacaatcgtt gtctttgaaa aacttttccg tgatggcatt gaagttgcgg tacatgtgga
     5521 tatcaatgat gacaatcaaa gtgttcgggt aaacaatcca acggttcata ccacagctgt
     5581 atttgaagac gggttgacga tcgcagaccc aacggggctg ttcaccttaa atgataccgt
     5641 tgaaattaat gagatccttc caggaaaagc ttacttggtc aaaggaatct taatggataa
     5701 agcaactggt gaagccttac ttgttcatgg tcaaccggta gaaagtgctg ttggctttat
     5761 tgccaaagca gaaaatgaaa cggtgatcgt accatttacc tttgatttga caggtttaaa
     5821 tggtcgtgaa atggtcgtgt ttgaaagtct ttttagaaat gacgaagaac tagcaaacca
     5881 taaagacatc aacgacaaag gtcaaacggt acgagtaacg aacccaagtg tacgtacaac
     5941 ggctacacat aaacccactg gcttgaaaga ggttaatcca ttaacgaaag tcaccattac
     6001 agacgctgtg aaataccacg atttgatcgt tggaaaaaca tacacagtca acggtgttct
     6061 aatggacaaa gtaacggaaa aaccagtgct agtagatgga aaagaagtct caagtacctt
     6121 aacgtttgtg gcagaaacaa ctgatggtac agtcaatcta gactttatcc tagacgcaca
     6181 tgcgttgcga agcaaagaag tcgtagtttt tgaagatctg tttagagaag gcgtattatt
     6241 ggcttctcac gcagatatta ccgacaaaga ccaaaccatc aaaatcgtca gtccaaccat
     6301 tacgacaaaa gctaccaatg caaatggtgg aaaagagatc ttagctttac caaatcaaaa
     6361 ggttgtcgaa tgggttcaag ttaatgggtt agcaatcggt caaatttaca aactgattgt
     6421 tcaaggctac ttaacaccag acggcacccc atttgaagga acacatgcgg aaaaagtctt
     6481 tatggctgat aaagaaatga tggaattctt gtttgaattc atcgttgacg gtcgcaacct
     6541 tgcaggaaaa ggattgagtt ttgcggaatg gctccaagcc aaaacagaag atgaaaaaga
     6601 aacgtttgaa gaagtcgcaa aacataacgt cgatctaaca aacaaagatc aaacggttca
     6661 atttgtggag gcaccaaaac caccgcaacc agtaaaacca aacgaaccac aacctgtaaa
     6721 agaagtgaaa caagctgcaa tggtacctat gttgccaaaa acaggctctg aagacaatgt
     6781 attatggatc atgattggta tgatctttgt ccttgcggca ggattgattg gttatgattt
     6841 gtataaagtc agagactaaa gaggacgaac aagcggttag gcaactagcc gcttgtttag
     6901 tttttagaaa gaatttttta tcaaaggcaa ataaattatt gtgttgagta gtaaatgatc
     6961 atgaggatac accaagaaat actgagtgta ataaataaga atttaaggat ccaaaagaga
     7021 tagaatataa ttatgtgata atacagtttc tttttttcta tagaagaaat cacgtttagc
     7081 tactcctaat tgctttctta ctttatttag ttctgctagg tagcagtatg agaattcaaa
     7141 atacattctt tgtactttga agaggatttt tttcgaatgt ttatttgtgt taattatttt
     7201 ttgtatttct ttggca
//
LOCUS       DACEOE010000131         7215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEOE010000131 DACEOE010000000
VERSION     DACEOE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.133"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(86..1051)
                     /locus_tag="IW774_002130"
     CDS             complement(86..1051)
                     /locus_tag="IW774_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1154465.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1285..2130
                     /locus_tag="IW774_002131"
     CDS             1285..2130
                     /locus_tag="IW774_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1154466.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2321..3508
                     /locus_tag="IW774_002132"
     CDS             2321..3508
                     /locus_tag="IW774_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1154467.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3514..3870
                     /locus_tag="IW774_002133"
     CDS             3514..3870
                     /locus_tag="IW774_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1154468.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3872..4210
                     /locus_tag="IW774_002134"
     CDS             3872..4210
                     /locus_tag="IW774_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1154469.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            4605..5639
                     /locus_tag="IW774_002135"
     CDS             4605..5639
                     /locus_tag="IW774_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1154470.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5632..6318
                     /locus_tag="IW774_002136"
     CDS             5632..6318
                     /locus_tag="IW774_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1154471.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6342..7190
                     /locus_tag="IW774_002137"
     CDS             6342..7190
                     /locus_tag="IW774_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1154472.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         2394 a         1285 c         1580 g         1956 t
ORIGIN      
        1 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
       61 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      121 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      181 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      241 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      301 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      361 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      421 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      481 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      541 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      601 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      661 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      721 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      781 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      841 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      901 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
      961 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1021 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1081 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1141 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1201 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1261 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1321 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1381 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1441 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1501 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1561 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1621 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1681 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1741 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1801 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1861 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1921 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     1981 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2041 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2101 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2161 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2221 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2281 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2341 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2401 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2461 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2521 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2581 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2641 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2701 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2761 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2821 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2881 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     2941 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3001 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3061 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3121 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3181 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3241 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3301 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3361 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3421 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3481 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3541 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3601 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3661 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3721 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3781 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3841 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3901 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     3961 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4021 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4081 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4141 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4201 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4261 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4321 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4381 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4441 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4501 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4561 aggctttttt gtttgaaaaa tcataagaga aagatgtgaa tggcatgtca ttaatccaac
     4621 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4681 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4741 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4801 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4861 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4921 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     4981 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5041 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5101 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5161 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5221 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5281 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5341 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5401 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5461 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5521 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5581 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5641 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5701 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5761 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5821 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5881 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     5941 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6001 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6061 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6121 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6181 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6241 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6301 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6361 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6421 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6481 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6541 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6601 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6661 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6721 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6781 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6841 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6901 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     6961 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7021 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7081 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7141 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7201 aaaaaagatg agaaa
//
LOCUS       DACEOE010000132         7175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEOE010000132 DACEOE010000000
VERSION     DACEOE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.134"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..716
                     /locus_tag="IW774_002138"
     CDS             24..716
                     /locus_tag="IW774_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1154473.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
     gene            complement(776..2794)
                     /locus_tag="IW774_002139"
     CDS             complement(776..2794)
                     /locus_tag="IW774_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ1154474.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2810..3806)
                     /locus_tag="IW774_002140"
                     /pseudo
     CDS             complement(2810..3806)
                     /locus_tag="IW774_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4004..5245
                     /gene="sstT"
                     /locus_tag="IW774_002141"
     CDS             4004..5245
                     /gene="sstT"
                     /locus_tag="IW774_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ1154475.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5392..6027
                     /locus_tag="IW774_002142"
     CDS             5392..6027
                     /locus_tag="IW774_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1154476.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6218..6958
                     /gene="truA"
                     /locus_tag="IW774_002143"
     CDS             6218..6958
                     /gene="truA"
                     /locus_tag="IW774_002143"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1154477.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
BASE COUNT         2305 a         1167 c         1398 g         2305 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt ttgtgtcgcc catgttgcac tgtactggtt acctgcaaca agatcatatt
      841 gtatcagacg ataatcttct agaatttctt tttgtttctc agttaaattt ttttcttgga
      901 taatttgact atcctctgta acaaagacct gttttccatt aaaacgatta gacaagcttt
      961 ctgtaggatc aattgttaat gcaggtagat gattagatac ttctgtcaac aacgcatagt
     1021 aaggagttac tttgatattc gcatgctcaa gagccatagc accaaagtta ttaggagcaa
     1081 caacttcttt atttaacgta gctgcttgtt cacgactata cttgttgtta taaatgaaat
     1141 aatctgtctg gtgctgttct aatccatatt ttttcgaatc attcccacta tagattgcgg
     1201 gtaaatggtc accataaaaa acaaaggtaa tcggtttatc gattttgtcg atttcttgga
     1261 tgaatttttt tactgctgta tcggtatagt gaaggccttg catataagtt tgtaattctt
     1321 ttcttctatt aggaatgaca gcagttcctt cagcagtgta gttcaattca ctatagtaat
     1381 catcataagg catatggttt tgcatagtgg ataattggat gaattgtgac gtttctgtat
     1441 tgctttgtaa agctttcaag gtttctttgt atgctgaatc atccgatatg cgtgggcttt
     1501 gttctatttt atccgtataa gttaatttat ccggactgtc taaataatag aacctgtcaa
     1561 aaccaaattt atcaaacact tgttttctgc gataaagagc agctgtaaaa ggatggatgg
     1621 ctattttttc atcgaataga tcggtaatat ttggacttat tttctgacgc tccactagct
     1681 gggtataagg tgtaactaac gaagcagaca ggttactgat actcaagcct gtcagtcctt
     1741 cccattccat gttagctgtt ccgccaccat aaccgacgga taacatcaat cctcctgttc
     1801 cttctgttat aatgcttttg atgtaaggaa tagggtctgt aggtactgtc agattaggga
     1861 cacgtgcagg atcactgaag ctttcactta aattgagaac gatcgtctga ttgtttaacc
     1921 agtcacttct tgtttggttg atttcatctg cctttgtgtt gtactttttc attattccat
     1981 taattttttc tttcgaatag ccagcaggtt cttccatgat tttaatgtcg atattgttta
     2041 ggaattgaat gactggtccg ttatctttta cggcgccttg ctggttaaag aaagtcttgt
     2101 ttactttaaa taaattaaac agcaagaaag aaggcgaatt tttgtgatta atgaaaaaga
     2161 cacccgaaaa acatcctaat aatacagcga tactgataaa tcgacgtttc caatgaatct
     2221 tcaaggaata ctgtttttgt agtctacgtt gtacaataat actagtaatc aataatagga
     2281 gaaggatcaa tccgcccacg attaataaga ctgggctaac catcgataat agttctgaca
     2341 atccggtcag catttttaaa tctgaaggaa agacaggctc ttcccgcatt tccattttga
     2401 ttactgtcga aatagtgagc agtaagttaa tgatcaaggt agcagtcaag gcgtaccaaa
     2461 aacgattgga aatcaacaat agtaagaaga aaaatcccat aataatgatg attgtcagga
     2521 tcatgggtgc ttgtcgctga gtaaatattt ttaaggcaac ctggatagtt tctttccaac
     2581 cttcttttgg ttctaaaacg aaaatctgca cgaaggtaaa gcagtaaaaa aatgctgcaa
     2641 ctaaaaaata acttttatga acaaaaagcc aatgaattct ttttttccaa cgctgagtta
     2701 attgttcaat cgaatcaaaa gcgaagtatt cgatgaaacg atgaatgaaa cggatttttt
     2761 gtatataaaa taaaaagata actaggaggg acactgctaa taaatagcaa ataaaaaact
     2821 gcccgatatt tatcagccat gccttccctg agtttggaaa ttcacgtttc ataaatgtag
     2881 atacgcgata agaaactaaa gggaaattac atataacttg agtgacaatc aaaaatacag
     2941 caagaaaatt tcctcttcct tttataccta atttatcaga tagcatttct aaaaataaaa
     3001 acgaagagaa agtataatac attgcgaaga gagaatatgg tttagaaaac ctcagcgctg
     3061 tatctgtatt tcctctgatg atgagtgaaa tttgtgtcat caaaataatc aaaccgaata
     3121 aaacaatcaa gctgataaat acttgaggaa gcttatagcg gaccttttct ttccagttcc
     3181 attctttcaa accataaccc attaaaaata aataaaaaat ccataaaata ctctgtccat
     3241 tctgtaatgc aaacgggtct ttattaaata gagtagagca gccaactaat ccaattgtta
     3301 aaaataatag tagatattta attgttgttt tcgataattt atttaaaaat ccaactgtat
     3361 aaggaatact acaaaaaagc aaagtacaag aaacagcata agtaaagtaa ttctgactga
     3421 taggtgttag gataagccaa taatcacgaa tattcaagct gccagcttgt acgtaaaaaa
     3481 aagatattgc aaactagtgt ggctattagg aatataaagg caaaacgact taaataatgt
     3541 aaaactttta aacgggcgat tttttgatat tggtaaccca tgtaaagtgg gatcaaattt
     3601 gcaccaataa caacggaagt ttgtaaaaat gtatagatgt agaatcgacc agggtttgtt
     3661 gcatatgtag gaatagtgac tgctgaaatc aatatacgct gtaaaaccat cattatacaa
     3721 gcgacaaaga ttgtcaggaa gccataactc tccttcttta tttttttcaa gaataaacac
     3781 ctcacaaaaa aattggacaa aaacataaga aaacgtttcc tgataagttg cccattaata
     3841 acatcctaac ttagaatatc tcaaaaaaca acgtgtagac agaaaaagta caaaattagc
     3901 aagaaatggt tgtttgtatt gttagaattt ttagaaaatt atacctttag tttctttgca
     3961 taaaatgata caatgattgt taaaattgtg aaagggtggt catttgttag agaagttttt
     4021 ggatatgtcg ttagttcaac gtattgttgt tggtatttta gtcggaatat tgtttggtat
     4081 ctttttcccc acatggacat ttatttcaat attgggaacg ttgtttgtca gttgcttgaa
     4141 ggcaatcgca cctattctgg tttttttctt gacgatggcg tcaattgcca agcataaaat
     4201 tgggaataag acattcgtga agcctatttt gatcttatat ttagtgggaa cattgctttc
     4261 tgcacttgta gcagttattg taagttcaat ttttactgtt cctattactt tacaagaaac
     4321 agtatctgaa aaagcaccgc aaagtttgga aagtgtttta tctactatgt tgacgaatgt
     4381 cacacaaaat cctgttcaat ctcttattga tgcgaattac cttggcgtct tattctgggc
     4441 tgtattgttg ggactagctt ttcgagcacg ttcagaatcg acaaaagagt tggtggatca
     4501 gatatctata gcgttgtcac aagtcgtaca agtgattatt gcatttgctc cgatagggat
     4561 tttaggattg gtttatcaat caattgctac gactggaatc gctggattgg cagagtatct
     4621 gcagctttta cttgttttga tcggtacgat gctgtttgtc gcattagttg tgtacccatt
     4681 cctcacgttt ttgtttatta aagaaaatcc ctatccattg attttctttt gtttgaaaga
     4741 aagcgcactt cccgctttct ttacccgaag ttctgccgca aatatcccga taaacatgat
     4801 gttggctgaa agattgaaat tgacaaaaga gtcttattcc atatcgatcc cgttaggtgc
     4861 tacgatcaat atgggcggtg ccgcgattac catttccttg atgacgttga ctgctgtaca
     4921 ttctttaggg attgaagcac cttttgtttt gaaagtcctt ttatgtattt tgtctgcttt
     4981 agctgcttgt ggagcttcag gtgttgcggg cggttcactg ttattgattc cattagcatg
     5041 tagtttgttt ggtatctcta atgatgtcgc gatgcaaatc gtcggtattg gatttattat
     5101 cggtgttatc caggattcaa tggaaactgc cttaaattcg tcaagtgatc tgttgtttac
     5161 tgcaatagga gagctaggcg cgcgtaaaag aagtggggaa aaaatcaaat tgaaagattg
     5221 tttagatgga ttaagtgaac gttgaacgtt agatatttag tatttttaaa atgattcgag
     5281 tttttaatga gttatataga tgttcgtgta aatagaggtt tctgttaaat caagataaag
     5341 tcgtttataa taacaattaa aagcataggt aattgttatt ggaggcgtat catggatttt
     5401 ttacgtaaat ttggtttttt ctttttatta ttaggcgtgc tgagtgattt tttgactccg
     5461 tacgtattag gattgtatta tcctgaatta gatcaaatga agagcgtgat cagtgtattt
     5521 ggagaggttt ccagtcctgt aagaaaagcg tttcttattt ggtctgtggt ttcagggatc
     5581 tttttcatcc tgtcattgcc tgcattgtat gctgcatttg taagtacatc tcgtgtgtta
     5641 gctaccctag ttacagcagc catcgcgttc tatggtatag gtgattgtat ctttacaggt
     5701 ttgttcagta tcaataccca tgaatcttcg tggaatgtct cgacatgggt ccataatatc
     5761 ggatcaggtt taggttacag tggtttttta ttgtttccag ttcttctttt cttgatctat
     5821 cgaaaaaatg ggcaaatgga cgtaagccac ttataccttg gactgtctgt gatcagtttg
     5881 ctttttgcgg cgatttatgg actggcaaga attccttcta taaattcttg gccattactg
     5941 aataaaatag gtttttgcca aagaatcagt tttttcttta attatgtgcc cattgcctgg
     6001 ttgagtatct tgcatctcaa acagtaaaac ttgagccgtc aaattgaaca gtcagcggat
     6061 acttcatccg gtggctgttt tttgtttttc tgaaatatat atttccacag ttttcttgta
     6121 tagcttctgt tttagagaag aaaggagcta tattttgtgc tacaatctta aaaaggtaga
     6181 gagaagacca aatggaagac agaggagcca ctaaaatatg agaaacatca agttgacaat
     6241 cgaatatgac ggaaaaagat attcaggatg gcaaagactt ggagatgacg ataaaaccat
     6301 tcaaggaaaa atcgaaaaag tattgcatca gatgacaaat gaagagatag aaattattgg
     6361 atcaggaaga acagatgctg gcacccatgc gcgaggacaa gtagcaaatt ttaaaacaaa
     6421 cacagaaatt ggactggcag aaatgatcga ctttatgaat cgttaccttc cgcgggatat
     6481 tgtcatcaaa agaatcgaag aaatgcctga gcgcttccat tcaagatata atgcggtggg
     6541 caaaaaatac agctattatg tttggaacaa cgtgattcct tcagcatttg agcgaaacca
     6601 cagcttttat ttccctcaag agttggatat ggacaagctg aatgcagctt gcgaaaaatt
     6661 agtgggtaca catgatttca ttggtttttc tgcattgaaa aaatcaaaaa aatccacagt
     6721 gcgtacgatt gatgaattat ctatcgaacg aaagggagaa atgcttcatt ttacttttgt
     6781 cggagacgga ttcttatata aaatggtgcg gattatcatg gggacattac tagaaatcgg
     6841 cacaggtgcc atgccgatag aaacaatcga tgaaatattt gaaagcaaag tgcggcaaca
     6901 tgcaggagaa accgtccctg cacaaggtct gtttttggat gaagtttatt atcgataaaa
     6961 atactttgat aatggatgaa acgcatgttt cacagtataa atatcgatgt taaataatgt
     7021 atgaagcaaa ttaaagaaat ataaaaaaag actttttagc ctgtttatgt gtacagctaa
     7081 aaagtctttc ttaaataaaa atctagtgag aatgttgtac ttctcttctc ggttgttttt
     7141 gtttagaaaa agtaaatcga taaagtaaaa ataaa
//
LOCUS       DACEOE010000133         6929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEOE010000133 DACEOE010000000
VERSION     DACEOE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.135"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(22..246)
                     /locus_tag="IW774_002144"
     CDS             complement(22..246)
                     /locus_tag="IW774_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1154478.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            497..2083
                     /locus_tag="IW774_002145"
     CDS             497..2083
                     /locus_tag="IW774_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1154479.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQEMVEEIE"
     gene            complement(2149..3924)
                     /locus_tag="IW774_002146"
     CDS             complement(2149..3924)
                     /locus_tag="IW774_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1154480.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(4137..5039)
                     /locus_tag="IW774_002147"
     CDS             complement(4137..5039)
                     /locus_tag="IW774_002147"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1154481.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            5318..>6929
                     /locus_tag="IW774_002148"
     CDS             5318..>6929
                     /locus_tag="IW774_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1154482.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGT"
BASE COUNT         2094 a         1352 c         1320 g         2163 t
ORIGIN      
        1 aaaaagtcgt tgggtcagaa cctatttttt cccttggttc ttcatttgct gcatcatctg
       61 gcgaattttc ttctcagatg gtttttgccc cattgacatc atcattgatc gcaacatgtc
      121 ctcattaacg ggaggatttt tttctaaata gtctttcata tatttacgcg ctaggaaaaa
      181 gccacctact gcgccgatta gcaaagcaat aatagcaata agtacgacaa tacctgttga
      241 taacatataa atctctcctt tctatttccg aattgctctc aatgacttat tttactaaag
      301 tttattgaaa aaagaaaggc gtattcttta gtttcatgca aaaaatagtg gtttttttag
      361 aggagaacaa aatttctttc gtcatctagc aagacacgcc acgaaaaaaa attaggcata
      421 ccgaatcttt ttctttaatt ttttcttttt gaggtgtaga atgtaatcgt agataaatag
      481 gaaaggtggt tttttcttga ataatcaatc caacaaacaa tttcataagc atcgacttat
      541 tgaacataca aatggtttat ctttagctga agtaaatgga acagtggatg taccaaaggg
      601 gaaaagtttt tttaaaacac ttcttgctta ctctggacct ggtgcgttag tagcagttgg
      661 gtatatggac ccgggtaact ggtctacttc gatcaccgga ggacaaaatt tccaatattt
      721 attgatgtca gttattttga tgtctagtct gattgctatg cttttacagt atatggcagc
      781 aaaattagga atcgtgaccc aaatggattt ggcccaagca atccgagcac gcacaagtaa
      841 gacattgggt gtcattttat ggatcttgac tgaattagcc attatggcaa cagatattgc
      901 agaagtcatt ggtgcagcaa tcgccctcta cttacttttt gatattccat tggtgattgc
      961 cgtctttatt actgtattag atgttttcgt tctcttacta ctgacaaaag tcgggtttcg
     1021 taagatcgaa gcactggttg tctgcttgat tttcgtcatt ttattcgttt ttgtctatca
     1081 agttggcttg tctaacccag attggcacag tttatttgct ggctttatcc ctaacagcca
     1141 tacattctcc gactcacctg tcattggtgg cgaaactccg ctaactggag cattagggat
     1201 tattggcgca acagtcatgc cacataactt atatctacat tcagcagttt cacaaactcg
     1261 gaagatcgat caccaagacg aagaagatgt agcacaagct gtacgttttt ctacttggga
     1321 ctctaatatc caattgtcga tggcttttct agtcaatgcg ctgctattga ttatgggtgt
     1381 cgctgtcttc aaaaccggtt ctgttcaaga tccttccttt tttggtctgt atgaagcttt
     1441 atctgatcca tcagctatga gcaatggtat cttgattgct gtagcaaaat ctggtatctt
     1501 gtccacttta tttgctgtag cacttcttgc ttctggccag aactctacta ttactggtac
     1561 gttgaccggt caagtcatca tggaaggatt catccatatg cgtatcccga tttggctaag
     1621 aagactggtc acacgattgc tttctgtgat tcctgtattg atttgtgttt tgtttacaag
     1681 tacaaaagga acgatcgaag aacataccgc tttgaataac ttaatgaatg aatcccaggt
     1741 gttcttggct tttgcgttgc cgttttcgat gatcccttta ctgatgatga ccaacagcca
     1801 agcggagatg ggtagccgat ttaaaaattc attcatcgta aaacttcttg gctggttctc
     1861 tgtgattagt ctaacttact tgaacttacg aggattacca ggacagatcg aaagtttctt
     1921 cggcgagcaa gccacttccg ctcaactagc attggctgat cacatcgctt atgtaatcat
     1981 tgttcttgtc ctcctcctgt tggcatggac gattggtgat ttatataaag gaaacaaaaa
     2041 atatgaacag cgtttgcagg aaatggtaga agaaatcgaa taaaaactga tttttccaca
     2101 caaagaccct tgatagttag gttaaaaatc tagctaccaa gggtcttttt atttagaaga
     2161 taaagctttt ttcagattta atttactgaa aactagcatg cttgttacta cgatgaattg
     2221 cgttaacggc acgcctgcgc cagtgtagac acttaaaaaa taatcctttt tttctggcgc
     2281 ataagaacaa aaccacgaga caccgaaaag aaaaaccaat tgtgtcagta ttaatccggt
     2341 gaccacaatc aaagaggaac tataataggc acgatcagct tttgcaaata cggttcctag
     2401 tatcataagt aatatccctg ctattgaagc aatcagcacg ggaatagcca gaacttggta
     2461 aatttttatg atgacattcg cgacttttgc tgattttgtt aattggaaat taggtgttct
     2521 cggttcatta ctatcgatcc aattctccat tagcggaagg tttaataatt cttgagcctg
     2581 tttgacttgt tcttgcgttc cgaaactata atttcctcct tgatggaaat ctttatatcg
     2641 ccatgcataa gcgtatcctg attgaaggaa atcgaatacg attggtagat cactcatcgt
     2701 ctttccatct cctgatccca tcagatagaa ctcttttttc tttttgatgg ttcccttgtg
     2761 ataggcttct tctaattctt ggttgacttt tttccaaaaa tcattggttt cttttgcatt
     2821 gttttggtaa taaccagatt cgcttaccac atctcgcaat gcccaaaaga tgatatctcc
     2881 gggtaattct tttacttttc caccttgagt ccacgcgtga gtcgtatata attcgtcaat
     2941 acgggtagaa acagtatgga atgtaggaga tgcggctaat gctttattta gtgcagcatg
     3001 tgagacccat atctcactgt tttctttatt caagttcgtt tcatcatcta ttcggatcag
     3061 ttgtttcatc aaacgtccaa attctccact ggaacggtca ttgatggttg caataccgta
     3121 tgttgtctca tttttatatt cgatccactg atagacgatg gaaaagccaa ctataggaag
     3181 taaaaacaat gaaagtttca gccattcccg tttcgagaga agaaagagtc gtttttttaa
     3241 cttctttact tctttgaaag aaaagtcttg ccctagcaag atctggacga aactgatacc
     3301 caaagctact acagtaaaag gaagaagcca gatagaatct tcgcgaatga accaaaagaa
     3361 aggaaaagcg acagaaagcc ctaaaaacca tggcagcaat tttctgctag aaccgtattt
     3421 gcggtaataa atagctagta aacaagcgat aatgatctca acagccggaa tcacgagggc
     3481 attacgatac gttcgagtgg aatattcatg tgtaaaacta atcggagaaa acaaaaaata
     3541 aaaatagaaa cttaaaagaa gatacttgtt cttcacgatc ggtttaaacg cgagtgcaac
     3601 caatatactg gcaaaaatac tgaacatgcc gaacaaaact gcataagaaa gatgcagttt
     3661 atgtgcaagt actaaaaata agctgtagga gattcctttg gaaagtgttg tagtcgtata
     3721 ctcccctagc catttaccgt ccaaaatatt ttgagcatag cgaatcagca gctggtcatc
     3781 ataggttgcc gacatgtctg ttccatagat tgctttacta tataaataca aacgaataat
     3841 tgtaaataat agggctatgc caagaataaa gtatgtatgt ttctttttta gttggttgat
     3901 caagggtttt aaagtgttca gcatcttttc gtccttccaa tcacgttttg ccacttgctt
     3961 gtgaaacttt tcgttttttt acttttctac cagtataccc tgtttgtcta aatttaaaaa
     4021 gaggggggag aagatgcatg atgaaaaaga ctgcaaaaag cactcctttg tttcttaaac
     4081 aaaaaaggac aaggcaatgt gcctggtcct tttttgctgc tgtcagatag aaaatcttag
     4141 agtatcactt cttctgctaa taactcagga aacacttctg gatcgaatat ttgtgctttg
     4201 aacatggcga tttcttttcc atatggtgcg taagctttct ttttatcttt gccgacatag
     4261 ggagtttcca agattttcga aatattgtca aacattggat catggacgat cttattcagc
     4321 gtgtcgaatc cgattgttcc aaaaccaata ttggcgtgtc ggtctttatg cgaaccttgc
     4381 ggatttttag aatcattgat atggagtact ttaagccgat ctaacccgat gatcttatca
     4441 aactcttcca ttacacctga aaagtcttct ttaatattat aaccggcatc attcgtatga
     4501 caggtgtcaa aagtaacgga taatttttca ttcaatttta ctccatcgat gatttgcgct
     4561 aattcctcaa atgttcgacc gatttccgta cctttacctg ccattgtctc caaagcgatt
     4621 tgtgccgtct gttcttttgt caaaacttca ttcaagcctt caataattct agcaattccg
     4681 gcttctgttc ctgcccctac atgagcacct gggtgaagtg taatttgagt agcgcctaaa
     4741 gcttccgcac gaacaatctc ttctctcaga aattggacgg caaaaggaaa catttctggt
     4801 ttgatcgtat tccccaaatt aatgatataa ggggcatgaa cgacgatatt tgttagaccg
     4861 ttttctgcca tgtacgcttt tccggcatca atattcatct cttcgatggc ttttcttcta
     4921 gtgttttgag gggcgcctgt atagatcata aaagtcgaag atccataact ggctgcttct
     4981 tctgctgctc ctaacagcat tttctttccg ctcatgctga cgtgtgagcc taataacatg
     5041 gatggattcc tccttgaaag gcaattgtta ctaacaattc ctatatattg gtaagatgat
     5101 atacaaagaa aatgattgat ttttctttgc cctgttctcc atagtaactg aaattttctc
     5161 taaagaaaag cttaagaact cttttataaa aaagatcatt ttctcacgtt ttaactcctt
     5221 taaaaaagaa attcctgcca aaaacagcct tgtctgtttt tttgttctat tatttgctat
     5281 aattagcgaa ataaaaccat tagttgaggt gacccttttg ccacgcaaaa atactcgaaa
     5341 aaaacagaat agaagaaaaa aagaaaaatg gtttgttcct aagattattt ttagagtttt
     5401 tcaatcattg actgttttta ttactgttct tcttttgatg ttcgcagctc taggtattgg
     5461 aataggcgct ggctattttg cctatctcgt cgaagacaca caattgccga ctaaaaaagc
     5521 actgcagaca gaactaggaa acattacaga aacgtcaaag attgtttatg cagataatac
     5581 agagatatca aaaatccaga cagatctgat gcgcacgact atttcatctg ataaaatttc
     5641 acctttattg aaaactgcaa ttatcagcac agaggatgaa tacttcgata aacaccaagg
     5701 ttacgttcct aaagctgttt tacgagcgtt agtatctgaa gctactggta ttggctcttc
     5761 tggtggatca actttgacac agcaattagt caaacagcag atccttacag atgaaacaac
     5821 attcaaacgt aaagcaaacg agatcattct ttctgcacaa gtagaaaaat acttttcaaa
     5881 agatgagatc attgctacgt atttgaatgt ctcaccattt ggtcgtaata ataagggaca
     5941 aaacattgct ggagtccagg aagctgcaag aggaatcttt ggtgtagatg caaaagatgt
     6001 gacgctgccg caagctgctt atatcgctgg attaccccaa agcccgatca cgtatagtcc
     6061 atacacgaac acaggtgcgc taaaagaaga tctttctgcc ggccttgcca gaaaagattt
     6121 tgttttattc agtatgtatc gtgaaggcca aattacgaaa gaacaatacg aagaagcaaa
     6181 agcctatgac cttacaaaag actttctacc acaacaaatc gcagaacaga ctgacagaga
     6241 attcttgtat tatactgtga tgaatgaagc aacaagaatc attgcacaac aattggctga
     6301 aaaagacaac gctgatatgt ctgattcatc ggtcagtgac gcttattatc aaaaggcaca
     6361 gcaaacaatc caaaataaag gatatacaat ccattcgaca atcgataaag atgtctatgc
     6421 tgcattgcaa aatggcgtag aaaattatgg atacatgtta gatgatggtg ctggcagtca
     6481 agtagaaacc ggaaatgtat tgatggataa cagaactggc cgcatctacg gattcgttgg
     6541 cggacggaat tacagtcaga atcaaaataa ccatgccttc gatacagaac gtcaagctgg
     6601 ctcatcaatc aaaccagttc ttgtttatgg acctgcaatc gacatggggc ttatcggatc
     6661 agaatcccgt gtatcagatt atgcaacaac ttggcaagaa ggagaaaatg ctggcgagaa
     6721 aatcgtcaat gctactaacg aaggatcgaa caccttccaa accgtccgtg agtcactaga
     6781 atggtccaac aatatcccgg cttatcactt atatcaagat gtgttgaatt ccggtggatc
     6841 gaaacaatat gcttacgagc actacttggc aaagatgaac tacccggcaa atgacaattg
     6901 gggcgttgaa tctgctcctt taggtacag
//
LOCUS       DACEOE010000134         6916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEOE010000134 DACEOE010000000
VERSION     DACEOE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.136"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            365..1237
                     /locus_tag="IW774_002149"
     CDS             365..1237
                     /locus_tag="IW774_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1154483.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1234..3192
                     /locus_tag="IW774_002150"
     CDS             1234..3192
                     /locus_tag="IW774_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1154484.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3443..4294)
                     /locus_tag="IW774_002151"
     CDS             complement(3443..4294)
                     /locus_tag="IW774_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1154485.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4417..4884
                     /locus_tag="IW774_002152"
     CDS             4417..4884
                     /locus_tag="IW774_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1154486.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5147..5629)
                     /locus_tag="IW774_002153"
     CDS             complement(5147..5629)
                     /locus_tag="IW774_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1154487.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5729..6592)
                     /locus_tag="IW774_002154"
     CDS             complement(5729..6592)
                     /locus_tag="IW774_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1154488.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         2074 a         1421 c         1251 g         2170 t
ORIGIN      
        1 tcctaacgga gaaatacagt taagtagagt ttttttcctt cgctttcagc tttttaacaa
       61 cgaataagat aagcaaaaga tccaacgatt aaataacata agtttcttaa tttatttctt
      121 ccttttattt ctgataattc cagtaaatta ctataattat aaaaaaagaa ttgacaaaac
      181 ataactgtct atataaaatt gattacgtta acaataattc gtttcatgtg actagatcat
      241 actaggcatg aaaaatctct gaggaacatg gtttccctag gaggtttttc gtgtctattt
      301 ttgtttggcc gcaatcaact ggtctagcaa acgtgaaaac caaatcttca aaaagggtgc
      361 gcgcatgaaa atcaaaaaga tctttaatca aaatgcagta ttggtagatg acggtggaca
      421 ggaaaaagta gctattggaa aagggattgg atttgacaga aaaagaaatg atttgatttt
      481 tgatcgtgat gtcgaacgga tatttgtcat ggaaccagaa ggtcaaatca agttgcaaaa
      541 tttacttaat cagattgatg aacgcttttt atttgcagca gaaacgatca ttgatcatgc
      601 ggaaactgtt ttgatggaaa agttgaatga acatgtattg atcgctttga ccgaccacat
      661 tgctttttct gcggagaaca ttaataacgg tatcgtcatc cgcaacaaac ttttaagaga
      721 aatcgaagtg ctttacagtg atgaattcag tattgctcaa tgggcagtag agtatctgac
      781 aaaagagctt gggattccat atacttatga cgaagcagga tatatcgcaa tccatattca
      841 cagcggacga agcggtcgga acaataacca tcgcagtatc cgtgaagtga cgattgtatc
      901 tgacatcatc catttagtgg aatcagaatt ggaaacagat atccattcag aaccattttc
      961 actgaattat tcaagactgg tgaatcattt acgactactt ctacaacgaa cccatgcgca
     1021 acaatatgct gttttagaca cggaaatcgt agaaatggtc aaacgaaaat acccagaaag
     1081 ctataagatt tctaaaaaaa tccgtgtgtt gctgaccaaa gaataccagc tcgcgatcac
     1141 gaaagaagaa ctgggttact tggccatcca tatcgagcgt ttaagaagcg caatcgtaca
     1201 aacaaatgtt aacaatcatg aggaggaaaa aaattgaaag catatctaca acgaatggga
     1261 cgttcccttc aacttccagt tgcggtctta cctgctgctg ctttactagt agggatcgga
     1321 aactggtggg catcttatag taacgacatt gtcgctcatt ttttacaagc tggcggtaac
     1381 gcagttttag gtcaattacc tttgctgttt gccgttgggt tagcactagg gatgtcagtg
     1441 gacaaatcag gtgctgctgc cttggcaggt ttggtcgcat ttgaagttcc agtaaatgtt
     1501 ttaaaaactg attcagtagc tactttattg aatatcaaac cagaatcagt tgatccatct
     1561 tttgctcaga tcagtaacgt atttattggt attattgccg gattgattgc ggcagctctt
     1621 tataatcgtt tccacgaagc aaaattaccg atggctttat ctttctttag cggcaagcgt
     1681 ctagtgccaa tcttggctgc ctttgtcatg ttgctgatca gtgcagtgct actgctcgta
     1741 tggcctccgg tttactctgc tttagtttct tttgctaaat tcattttgag tttaggctgg
     1801 ggtggtgcag gtctttatgg cttcttcaac cgtttactga tccctacagg attgcatcaa
     1861 gcaatgaact ccgtgttctg gtttgacgta gcaggaatca atgatattgg taaatttttg
     1921 gcttctgaag gaacaaaagg cgtaacagga atgtatcaag ctggattttt cccaatcatg
     1981 atgttcgggc ttccagcagg agcatatgct atttacagaa atgcacgtcc agaaaacaaa
     2041 gcaaaaacag cttccttgat gctagcagca gcttttgctt cattctttac tggtgtgaca
     2101 gaaccacttg aattttcatt tatgttcgtt gcttggccat tatatgtctt gcatgcaatt
     2161 ttcactggga tttctttagc agtgagtgca ttcttccatt ggacagcagg atttgccttt
     2221 agtgccggat ttgtcgatta cttcctatct ttgaaaaatc cgattgcgaa tcagccattg
     2281 atgttgatcg tccaaggatt agtaacagca gtgatttact actttggctt taactttgct
     2341 atcaaaaaat tccatctgat gactcctggc cgcgaggaag cagatctttc agatgacgat
     2401 actgcaacga ctaattcaga caataaatat gcggcacaag caagcaaaat ctacgctgca
     2461 ttaggcggag ccgacaatgt aacttcgatc gataactgta ctacacgcct tcgtttgcaa
     2521 gtcaaagata caggcctgat cgatcaaaat aaaatcaaag ccactggcgt acctggtatg
     2581 aagatcatcg acggaaaaaa tgcgcaagtc atcgtaggta cagaagtaca attcgttgca
     2641 gatgagatgg caaaactaca tggtggtgct gcagcacgac ctgcacaaac aaacaccgtg
     2701 gttaaaacag aaacatttgc cggtgaaacg atcgaacaag atatttacgc tatcgcaaat
     2761 gggaaactga ttccaatcac tgaagtttca gatgatgtat tcgcggaaaa aatgatgggt
     2821 gacggctatg ctgtattacc agaaaatgga gaaatcttct ctccaatcgc tggtacgatc
     2881 acaaacattt tcccaacgaa acatgctgtc ggaatccaaa cagatgctgg tatcgaagtc
     2941 ttgcttcata tgggcatcaa cactgttgac ttaaaaggcg aaccattcac tttatatgta
     3001 gaagaaggtc aaaaagtcgc aagaggtcaa ttgattgctt tagttgatct tgccgctatt
     3061 caatctgcag gcaaaaacac ggatatggtc gttgtattca caaatggcga caaagtccaa
     3121 agcttagaaa tcgaacccgc acgtgatgta aaagcaaatg ataaaatcgg ctctgtttcc
     3181 aacaaagcct gagccatcgt cttccccaga cttttttatc gataaacaaa caggagctga
     3241 gacaaaaatc ttgtctcagc tcctttttcc gaataaaaac ggcgttctcc tgccaactcc
     3301 gcggtaataa gtccagctaa acgaaacata tgaagagcta ttttttattt gactcttctt
     3361 attacttgat gcagaacagc tttttcacaa aatttttctt catactcaaa actaatcggc
     3421 tttttcacaa cctctttata cattattcct gatccaatat ttcttttaat gctgcttcaa
     3481 gagaagttaa tggttttccc aatacttttt ctaaatcatc agaggaaata gctaactgat
     3541 cctttttaat atcatgttgg atcgctaaaa acatgtcagc tgcttcttga gggaatccgg
     3601 cttcttttag attttcgaca aacgctgcat cacttgcttc aataatttct aactctttac
     3661 ctgtcgcacg ctctaatgcc ttggctaatt cttcatagtc agttagattg ccggataatt
     3721 ctaagatttc tggagagtca ctttctacta gtacttttgc tgcagcttct gcatattcac
     3781 gtttcaatgc ccaacctgtt tttccatttc ctgcagcata aacgaattga ccagttttta
     3841 aagcctgccc aatgataggt aattcattct ctacatacca attattccgt aaaaatgtat
     3901 gggaaatacc tgaatcctta atcatttttt cagtgaattg gtgatctggc gctaatacac
     3961 tgatcgagtg atcggcatct gcaaaacttg tataagcgat ataagatacc ccagctgctt
     4021 gtgctgcctt cactacattc ttatgttcgg cttgacgatt tcctggaacg ccagagacaa
     4081 aaagcaatcg atctattcct ttcaacgctt ctttcattga gtctaaatca tcataatcac
     4141 caatacgtat attaaatcca gcttctttca gtgcttctcc tttttcttca ctacgtacta
     4201 gtacatagat ttcattttgt ggaaccatct tttttaagaa ttccagcgca taaccaccaa
     4261 aacctccagt tgctcctgtt actaaataat gcatatttgc cactccctat tctttttatt
     4321 tgttaagatt tacagctgta catttataaa ttacaccttt atcataaaga gtgtggtaag
     4381 ataagtcaag caaaaaaaat gaaaggatga caactgatga agtattctct gcaatttagt
     4441 gatgcaatcc atgtacttgc ctatattgaa atgtttcaag atacccgatc actttctagt
     4501 gagatgattg ccagtagtat cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag
     4561 ctcaaaaaat ccggtttgat ctttacaaaa accggaaaac cgcagccttc ccttgccaaa
     4621 agtccagatc agatttcttt actagatgtc tatcgcagca tcgaaggtaa tacacagctt
     4681 atccaagtcg atccaaaaac aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct
     4741 ttagaagaaa gctacgctcg gctgcagaaa aaggcagaag aagaaatggc taagatcaca
     4801 cttgatagtc tgatacataa aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca
     4861 ggcctcgtag agagattttt ataattctct ctactgtgcc tgcatttttc tgatcggaat
     4921 cgtctacata aattctttta ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct
     4981 ctctaatgct caggtcaaag cgctgaagtc atgacgtctt gataaacggt gttcaagtag
     5041 ctgaccctcg gcattagttc tgaaaaatgc aaaatatgga aagctatttc ccatttttcc
     5101 tctctaatgc tcaggtcaaa gcgctacttt cacgacctct atattatcat ggtttattga
     5161 ctaccggtat ccagatttct cccatatatt tcccgttggt aactcccatt tcgactacag
     5221 catttggacc cccaacatag gcgtaatctt ctacttctct taaaacatgc ccaaaggccg
     5281 tattcgtcaa atcatttctt aattcttcag gcgattctgc taaagcgctt acaacaagat
     5341 acgttccttt tgggaatggg atgattcgcg tagtgtttgg aacatcctga ctcgtttgaa
     5401 caccgacata gtacatcatt ttcccgttat agggttcgtt gactgtaaag aaataatgat
     5461 tttcagcagc ttgtttcaat gcatctattg ttccattacc gataactttt tgctcaaatg
     5521 cttgtttctc tgcagccaaa ccgacaaaat ccgtattgct actcttcaat tccacaccaa
     5581 tacccgtcac cgtaaaatct ggtttttctt cgatcctata ctttgccatt tctcccaccc
     5641 tttctattat attaagttca tcttacataa aattaaaggg aaatgacacg attacgctct
     5701 tttttctagg gatattttta ttttttaact atttttttgc tttcactgca atccaaatct
     5761 cactgtaaac attactgaaa tcctctcccc attttgtaaa agagatctcg ggagcttcga
     5821 ctaacccata atctgaagaa ggaagccatt ctgcatagat tttcccccaa gtatcttgta
     5881 aagtttgggg aaatggccct ttattaggaa aaatggccca agtgtgttct tctatagcaa
     5941 gcatttctaa atcagcgtac ggattttctt ttgtcgtagc aaagccaatc atgtgcatca
     6001 aactgccctt ttcttccaag cggtctccat caaatccata agaaacgttg attacttgat
     6061 ttggatataa atctcctaac ttatgcatct cttccctttg agtctccgtg atcgtctgcg
     6121 ctaacttttg tatttcttga ttgactcctt caaatttgac aggaactttt ttcgccacac
     6181 ctgccacata aaactgttct ttcttttcga tacgaaattc catcgatttt cctcctctga
     6241 catcaataaa aaatgtcaag cgtggaaaag atttttgtat gccgtatctg ctgatttctg
     6301 aaggcagata tcctgaccaa tcacgaaaag ctcttgaaaa accttcgact gtttcatatc
     6361 catatctaaa cgccgtttct gtcactcttg atccatctag aagatcgcga ttagccaaag
     6421 ccaatctacg gtttttgata tagtccatta gcgacatccc tgcaataaac gtaaagatgc
     6481 gttttaattg atattcagag attcctgtat tttcggctat tattttatag gaaagtcctt
     6541 ctgtcaaatg ttcttcgatg taatcgatca attggttcag atctttaagc atatttatct
     6601 tcctccttga ttgccttttt atcatattat caaagaaagt caattacttg atgaatcagg
     6661 aaccaattta gtcagatgta gaaaatgttg tatggatcta aagaaaattc tcaagcagcg
     6721 tttcgtaacg gataattggt attatctgcc tatctatgtt ttacctttag aaaaataaat
     6781 tttggggaag acagattcta agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt
     6841 aatccgctta ctacctactg tacaaagcaa attgttgttc aaactgctga tcagaacgct
     6901 gcttggattt ttcttt
//
LOCUS       DACEOE010000135         6891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEOE010000135 DACEOE010000000
VERSION     DACEOE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.137"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            115..2112
                     /locus_tag="IW774_002155"
     CDS             115..2112
                     /locus_tag="IW774_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1154489.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTSAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2298..3107)
                     /locus_tag="IW774_002156"
     CDS             complement(2298..3107)
                     /locus_tag="IW774_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1154490.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            3400..4836
                     /locus_tag="IW774_002157"
     CDS             3400..4836
                     /locus_tag="IW774_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1154491.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            5006..6202
                     /locus_tag="IW774_002158"
     CDS             5006..6202
                     /locus_tag="IW774_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1154492.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPLMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(6264..6449)
                     /locus_tag="IW774_002159"
     CDS             complement(6264..6449)
                     /locus_tag="IW774_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1154493.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            6680..>6891
                     /locus_tag="IW774_002160"
     CDS             6680..>6891
                     /locus_tag="IW774_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ1154494.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYT"
BASE COUNT         2122 a         1240 c         1440 g         2089 t
ORIGIN      
        1 ttagaaaaaa taagaaaagg tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat
       61 gttccttttc gcttaaattt tctttttttc attatattta cgttatttgt tgcgttgatt
      121 gctagattgg gttatttgca gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct
      181 acttctacga ttacggtcca agaaagtact cctcgcggaa tgatctacga ctccacagga
      241 aaagctttag ttacgaataa agcgaatcag gccatcacat atacgagagg aacacaagtt
      301 acggctaaag atttgcttga aatcgcaacg aagctgaact cgctgatcga tgttccggta
      361 gatgaaaatc tgacagatag agataaaaaa gatttttggc tggctgatcc caaacatttg
      421 aaagaagcca caaaaagact ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat
      481 agtgcgatgg tcaataaagt cacagctgat gagattaatt taaacgagga gcaattaaaa
      541 attgctacca tctttaaacg gatgaatagt gcatatgaat taaacactgt ctatatcaaa
      601 aactctggtg ttactgatca agagttagct gttgtggcag aacgcgcttc tgaacttcca
      661 ggagtttcga ctggcacaga ctggacacga gaatataatg cagcaagttc actgaaaagt
      721 attcttggca gtgtgacgac tgaaaagcaa ggacttccag cagatgaagc agagaaatac
      781 ctagcaaaag gttattctcg gaatgaccgt gtaggacaaa gttatttgga aaaacaatat
      841 gaagatgtcc tgcaaggaac caaaacccaa tatgaagttt cccttgataa tgaaggaaat
      901 gtttcgaatc aaaaagaaat cttctctggg gaaaaaggtt ctaatttaat gttatctatg
      961 aatgcagaat tccaatcaaa agtagaagaa attttgaaaa ggaattatca aacattgatc
     1021 aacaacggca aagcacagta ttcgcctggt gcttatgcag tggctatgaa tcctcagaca
     1081 ggagaagtgc tagcaatgac tggtttcagt catgaacaag gttccaaaga aatcaccgag
     1141 aatgctttag gcacgattac tagcgccttt gatccagggt ctgtagtcaa ggctggtact
     1201 ttgacagcag gctgggcaag taacgcgatc agcggaaatc aagttttgat tgatgagccg
     1261 attcgtctgc aaggtgcttc agaaaaaagc tctgtcttta atcgaagcgg acaagttgct
     1321 cttgatgcag tcaaagcatc ggaattgtct tctaatacgt atatgatcaa agtggcgtta
     1381 aagatgttag gactagacta tacacctgga atgggactac ctagtttaga tgaagaagct
     1441 aaagcttatc agcaattgag agactctttc aaagagtttg gtcttggtac aacgactggg
     1501 atcgatttgc cgaatgaatc tcctgggatc tcacgttctg ttgattatat gaagaagttc
     1561 aatgcggata atggcaaaga atggtatgct ccaggtaact ttaccgattt ggcatttgga
     1621 cagtttgaca catatacacc aatccaatta gctcaatatg cttcaacagt cgctaatggc
     1681 gggaaacgag tacagccgca cctagtcaaa gcgatctatg gcaatgacga aaatggaaac
     1741 ttgggtgaag tgaagaaaga aattggcaca acagtagaaa atacggtcaa tatttctgca
     1801 gaaaatatgg cgatcctccg tgaaggattc caccaagttg tccatggtac cgatccttac
     1861 acatcagcca aaccattagc cagtgcgaaa atggaccttt ccgcaaaaac agggactgca
     1921 gaaacggttg cggaaggtca tccagatatt gcgacagtca atagtaatat cgtggcatac
     1981 ggaccaacag acaatccgga aattgccatc agtgttgtct tgccgaactt attagacgaa
     2041 caagaccaca taaacttgac gattgctaaa gaaataatgg acgcgtatta tgatatgttc
     2101 atggcaaaat aaaaaagagg tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt
     2161 ttgtgaaaat agcttctcac atttttgctt aaatttgact tcataccgag aaactggctt
     2221 ttgaacaccg tttactcggc aaaaaaggtt gtgacaaaaa ttgtcacaac ctcttttttg
     2281 tggaatctaa ttctctatta tagtttaagt agtttttcca gataaacagc taaaccgtct
     2341 tcctgattgc tcaaaggagt gacatcattt gcaatgcctt tgagttggtc agtaccgtta
     2401 gccatagcta ctccccaacc tgcataatcc aacaattcca aatcattatg ctcgtcacca
     2461 aaggcaatga cgtctttttg atcgatagac aggtagttag caatctcttg cacgcctttt
     2521 gctttttgga ttccttttga aacaatctct aagatggcag taggtccacc ccatgtccga
     2581 acatcgacat aactgccaaa ttgacgtgtt aattcagcag atacagctcc tgcaaaacgt
     2641 ttgtctgttc ggacaagcaa agatgtagga tttgtcgtca aatttttagg agaaagcaaa
     2701 ttcttctctc ctggacgaga agaagcaaaa attttttcat caaagaaatc aaaactgtca
     2761 ataaaaaaag tatcacgatt ttcagcagcg ataaaatcta aatttagctg ctttttttgc
     2821 gataagattt caaaagcgat ttcacgattg atcaacgttt ctctttcacc atcccagtgc
     2881 tgattaggga tgtgtactaa tgcgccgtta aaattaacca tcggtgttgt taattctaat
     2941 tgttggtaaa actgatggct cattcggaat gggcgtcctg tagcaatact gacatgatga
     3001 ccggcattga ttgctttttt tagagtctct tttgttttag atgtaatcat cgaatcttga
     3061 ttgagggtag ttccgtccag atcgatggca attaattttt tattcaataa gggtccttcc
     3121 ttcctcaact agcatttgtt ttagtctagc atattttagg taattaacaa gagcaatcag
     3181 ctattcctaa caaaaactac agttatggct atataaatag ctaaacataa gctaaacacg
     3241 aacgttaagt attattttcg ctttttttgt taaaaaaacg ttgaaaagcg aatgaaacgt
     3301 ctatataatt tgaaattgtt cgatggattt gccctatttt acttattttt tattattata
     3361 gttttccatc agaaaaaaat tcagggagtg gaacttttta tggaaaagtt tttcggttta
     3421 aagaaaaaca atacaaatgt atcaactgaa ataatggcgg gagtaacgac attctttgcc
     3481 atgagttata tcttattcgt aaatccgacg attttgtcag catcaggtat gcccttccaa
     3541 gcagtatttc tagcgacaat cattgcatcg attattggta cgctggtaat gggattgttc
     3601 gcaaatgtac catatgctca ggcacctggt atgggattga acgcattttt cacttttacg
     3661 gtcgtttttg ggcttggtta ttcttggcaa caagccttgg caatggtgtt tatttgcgga
     3721 ttaatcaata tttttattac tgtcacaaat attcgaaaaa tgattattcg ggcgattccc
     3781 gaaagtcttc aacatgcgat tggtggcggg atcggtattt tcgttgcgta tgtcggaatc
     3841 aaaaatgcag gattcttatc tttttcagca gaccagtcag ctatttcgag ctctgtcgtt
     3901 gaaggcggaa aagcgacaaa tgttacgata aatggtggaa tcgtccctgc gttagccaac
     3961 tttgacaacg ctcctatctt attggctgtg attggtttgg tactgacagc cattttagtt
     4021 gtaaaaaatg tccgtggggc tattcttatc ggtatcgtag cgaccacagt ccttggtatt
     4081 tttatgggtg ttgttgattt aagttccatt gattggcaaa cgaattcgtt aggtaattca
     4141 tttaaagaat taggaacaac tttcggtgca gcatttggtt ctgaaggcat gcaatcttta
     4201 ttcagtgatt cttccaagat tcctcaagta ttgatgacga tcatcgcatt cagtttatct
     4261 gatactttcg atacgatcgg aacatttatc ggtacaggac gccgcacagg tattttctca
     4321 aaagaagacg agatcgcgtt ggaagatggt cgtgggttca aaacaaaaat ggataaagca
     4381 ctatttgccg atgcgatcgc tacttctgtg ggggctatct ttggtacatc gaatacaaca
     4441 acttatgtcg aatctgcagc tgggatcggg gccggtggac gtactggttt gacttcagta
     4501 gtagtggcag tgctattcgc attaagtagt ttattctctc cactgatcgc cattgtacca
     4561 aatcaagcaa ctgcgccagc attgatttta gttggagtaa tgatgatggc ttcttttgca
     4621 gacatcaaat ggctagatat ggaagaagct ttaccggcct tctttgcatc gatctttatg
     4681 ggattgtgct atagcatttc ctatgggatt gcagcaggat ttattttcta tacaattgtg
     4741 aaagtaatca aaggaaaata caatcaagtt tctgttgctt tgtggatcgt tgatatttta
     4801 ttcattatca acttcatcat tctagcaaca atttaactaa agagaaggga ttggaacagt
     4861 atttgttctg atctcttttt gttttacata aaaagaacgt tacatataaa atgacctttg
     4921 cgggattttt ctctttttac tggcgagatc gttgcttgtt tattcgaaaa taggtatgat
     4981 aaaagagacg aaagagggat gatagatgga gattaattgg agaaaaaatt tagtgattgc
     5041 gtggattggc tgttttttca ctggtgcaag tattagttta gttatgccat tcataccagt
     5101 ttatgttgag caactaggaa caccaaaaga tcaaatcgaa ttgttttcgg gactagcaat
     5161 ctctgtgact gcttttgcat ctgccgtggt tgcaccaatc tggggaaatc tagctgatcg
     5221 aaaaggccga aaactcatga tgatccgtgc agcagcaggg atgacattaa caatgggatc
     5281 tttggctttt gtaccaaacg cgtattggct tttgatcatg cggttttgga atgggatttt
     5341 gtctggatat attccaaatg ccacagcgat gattgcttca caggcaccta gagaaaagag
     5401 cggatgggcg ttaggaacat tgtctaccgg agcgattgct ggaaatctga tcggaccttt
     5461 aatgggcggt gcactggctc agtggtttgg tatggaaaat gtattcttga tcacaggggc
     5521 tttgttgatg atcacaacgg tattgaccat tttcttagtc aaagaagatt tccatccaat
     5581 cgagaaaaaa gatctgataa gcaccaaaga aattttttca aaaatggatc atctctctat
     5641 tttgatcggt ctcttcatta cgaccttgat tttacagata ggaatcacta cgatcagccc
     5701 tattttgacc ttatatattc gtgaattgag cggaagtaca gaaaatatcc tttttgtcag
     5761 cggcttgatt gtttcgatag ctggtgtttc cgctgtcttt tcttcaccga aactaggaaa
     5821 actaggggat aagattggga atcaaaaagt tctaattgga ggactcattt tatcattttg
     5881 ctgttattta cctatggcat tcgtcactac accgctccaa ttaggtatac ttcgctttat
     5941 tttaggcttt tcaactggcg ctttgatgcc ttctgtcaat acactgatca gtaagattac
     6001 gcctcaagaa ggagtcagcc gaatctatag ctacaatcag atgtttagca actttggaca
     6061 agttttaggt cccatgctag gatcgacagt cgcgcatgct tataattaca gtgctgtttt
     6121 tattgtgaca agtttgtttg ttctgtcaaa tattcttctt tctttattca actttcgtaa
     6181 agttcttcgc agcagattat aatggtggtg aggagtagaa aataaaaaag agcttgagaa
     6241 atctcaagct cttggctgtt tttttacgaa tgggcagagg tttcttttct tggcgcaact
     6301 gcaccaatca aaaggataat gacacccgca atgaatgaaa tgattgttgt caaagtaaaa
     6361 tcatatgttc cgcttgttaa tgcagcacca atataaccga ctacttgacc tagaacgaca
     6421 gcccagaata atgtaacgat atatcgcata aagaacacct cttttctatc ttaattttac
     6481 cagattttct ttgaatgtaa agaaacgaga ccaaatctca ataaaatttg agttcttgcg
     6541 aaagaactca ttcctacaga ttacaagcaa gcaaatgaag tggaataatc aacttcagcg
     6601 cctgctatct taatgaaagt aaaaggatca aaccttttaa cgctagtgaa ttttaacgaa
     6661 aaaaaaggcg gaatgcaaga tgtttttccc acaactttat acaattactt cttattctct
     6721 tttacaaagt acgatacgta ttcgagaata tgtacaagaa gcaaaaaaac aaggctacac
     6781 ttcattagcc ataacggata aagatgtctt atacggtgca cctgaattct atcgtatttg
     6841 tgtagaagaa ggagtacagc caatcattgg attacatctt agttatactt a
//
LOCUS       DACEOE010000136         6827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEOE010000136 DACEOE010000000
VERSION     DACEOE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.138"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            184..540
                     /locus_tag="IW774_002161"
     CDS             184..540
                     /locus_tag="IW774_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1154495.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            550..1449
                     /locus_tag="IW774_002162"
     CDS             550..1449
                     /locus_tag="IW774_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ1154496.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            1966..2697
                     /locus_tag="IW774_002163"
     CDS             1966..2697
                     /locus_tag="IW774_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1154497.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2842..4869
                     /locus_tag="IW774_002164"
     CDS             2842..4869
                     /locus_tag="IW774_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ1154498.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            4977..6074
                     /locus_tag="IW774_002165"
     CDS             4977..6074
                     /locus_tag="IW774_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1154499.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6381..>6827
                     /locus_tag="IW774_002166"
     CDS             6381..>6827
                     /locus_tag="IW774_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1154500.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT         2397 a         1102 c         1386 g         1942 t
ORIGIN      
        1 ctttttgggt ctttttccgt tcaagaaact gaacgaagaa aagggaaagt tgtctctttt
       61 gtgataagag aaatcataat aaacgaatga cttactttcc aataaattga gaacagtcag
      121 aaccagacaa attagttgca tctgatgaat aaagtgatta ttctatgaca tgaggtgata
      181 cagatgaaat tagaagtgac aaaagaagca caagaagtat tgaagaataa attagaagca
      241 gacgatcaac ttttattaga tatcgaaaac ggtgacggtc catttgcaga aagccaagtc
      301 agttgccaat tggatacggc atttcgattg atcattgtca aaaaagatac acaaacagat
      361 ctaagtcttt attctgtagt ggctgataca ccagttggac caatcaaaat caaagacagt
      421 gcaatacttt acatggatga tccatccaca cttgctttag aaccaactta taatagttta
      481 caattaaaag gaccaagcgg cttgagaaaa ggcaacttgc aagttgttcg aaaagactag
      541 agggataaaa tgggaaaatt ggcgatcatc agcgacctac atgtggacat caataaattc
      601 ggcgaatcag agctgatgca gctttggcaa gttttgcaaa agcacaagat cactcggctt
      661 catctagccg gagatacggc taataaagta gaccgttgcc tagctgttgt cgaatttttc
      721 gtcaataaag gattatctac gacatttcat ttcggcaatc acgagttggc agatgtgccc
      781 aatgaacaga tgatggacca ttttccagac ccacattttt taaatgaacg gtatattgct
      841 ttaaatgagc agaccgttct tttagggatg aatggctggt acgactatca gttttccgaa
      901 ttgaaagatt acgaggcaat cgtacgaatg aagcgtttgt attggtatga tcggttcatc
      961 cagcgtggga aaacagatct cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg
     1021 ctagaccagc tggaagcaaa aaaacttgag gtgattttat ccactcattt cgttcctaaa
     1081 cgggcattta tcatgtatca atctgctcct tatgagcgtt ggaacaaatt aaatgcattt
     1141 ttaggatcag aatcattcgg agaattactt gaccgttatt caaatatcaa acaagtagtt
     1201 tttggccata cgcatcgtcg ctttgaagat aaatccatcg gtcacacgat ttacagctgc
     1261 agaccttttg gttattatta tgaatggcag ctgacccgag attttgtctt gaaagaacag
     1321 ttgctagaca gctatgatcc tatgaaatta cgtgtcctct tgcgtgccca tcacccagct
     1381 tttcaggcct atcaaacaca acatttgcaa gaggaatttg aacaagcgat gacaatcgtt
     1441 ccgtattgag acagcattag taatgtgtaa aagaaaatac cttgtaaaac ataaggataa
     1501 gtaagagcag tagatccatt tatcgtggat atgctgcttt ttttgcggaa aataatcagc
     1561 gaatgtttat ttgatcggat gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc
     1621 tttttttgaa cgataatcac aaaaaaaaat ataatataca ttttttattt aattataatt
     1681 aatattgatt gaagtctagg cagtcaagtc acattttgat agataggagg gggacttacg
     1741 aattatttta aaggctatga cagctgtatt tctagattgt gtttgcatca agttgcaata
     1801 aaaactagaa atcaaatcgg gctgataatc atttataact aatcgataca taggaccaaa
     1861 tataggtcta tgttctttta tttcataagt atcgtaaaac agaattttgc tatggattaa
     1921 tccaatcgac tgatatgttt agtaaataaa gaaagaggaa tgtcaatgat gaaaaaagaa
     1981 atcgtattaa cagcagcaat gatgttattc agcatggtag caagtacgac ttttgtttct
     2041 gcaactgaag tatacccaaa ggaatataac acagaaggaa cgattacatt tgaagcagga
     2101 gatgagggtg taactcctcc tgttgatcca gagaatcctg atcccaataa acctgtcgat
     2161 ccatcagatc ctccaagccc tggaacagga ggggcattat cgattgatta tggatcaaaa
     2221 ttcaaatttg ggactcaaaa aatttctact gcggataaaa cgtattatgc agctgctgat
     2281 gtgatgaacg atgggtctag aaaaccgaca tatgtccaag tgacagatag gagaagtacc
     2341 ttatctgggt ggaaattaag tgtctctcaa ccagaacagt ttaaaactgc cagtggggat
     2401 gaactagttg gagcacagct gaaatttacg aaagggcagg ctgtttcttt ggttgatcca
     2461 acatatacgc cgcaaacggt gaattctgaa ttaacgttga caccgggagg aaacaatacg
     2521 ttagcaatta atgctaaatc aggaacaggt gtggggactt gggtctatcg atttggtgca
     2581 aatgaaaatg aaaatcaaga tgcggtacag ctttcggtac ccggtaaatc tgtcaagtta
     2641 gcgcaacaat attccactaa attagtttgg acattagaag atacacctaa caactagttt
     2701 actgaaaaga actgttaaaa ataaatacgt agtcatattg tcttgcgagg taatcgagca
     2761 aaaattgtca aaggctaggc taaatttgtt tggatacgaa tagttcatct aggaatttaa
     2821 cgatcaggag gaaaagatat attgaaaaag aggaggatcg tgctcatttt aagcagtctc
     2881 ttagtgacta atatctttcc gatcataggg tactctttgg aattgaatca gttggagcat
     2941 ggaaatagaa gagacgaaga aagcaaactc acttcgacat tcgacagttt aatagaatcc
     3001 aaccagacac aagaaagcag tgaggattta agtatttcga gtagtggaga gaccactgac
     3061 aacgtagaag ctgagggttc ggacaaacaa ggactagaag atgcaccaat ttctgcggtt
     3121 gagctgcctg acgatcccaa tattatttct atcgataaaa tttttcaaac acctatcggt
     3181 actagcacgt ccatattgga aaatgggaag ttactgcagt tgaacccagc agaaaggtct
     3241 caaagaggag ctatttggtc aaataagaag attagtttac tctcggattt tacatttaaa
     3301 tcttatttat acttaggaaa tcaacgagat agagcgggag atggcatgac ctttacattg
     3361 actggcgatc ctagaatggc tagtaatccc agtcaagtta ttggatcgcc aggtatggga
     3421 attggctcat attctactcg ttctggccaa ccttacattg cgaatgcgct ctcgattgag
     3481 tttgatacgt ataaaaatag tggttcttct gatcgaatgg atcgagagat aactgctgat
     3541 aatagagggc atggacatgt cgcttttgtc acgcctaaac caaataataa taattattct
     3601 ggagagcata ctggcgtgac ggtagctcct acatttcttt ctaatggcac atggcgcttg
     3661 ttaataatta aatgggacgc ggagcaaaga agtcttactt atgatttgga aggtgtcggt
     3721 cgctcttctc ggattatacc tgatctcatt aggcagtttg gttctacaga agtaaattgg
     3781 gggtttactt cttcaacagg tacttttttt caagaaaatg ctgtcgctgt cacgcgactt
     3841 ccatcgaatg tcacatcaac agctgatgta aaagtcaacg atagtgatta tggggccatc
     3901 gctgaagctg gaggaaacga taagataact atcagaaata cattgaaggt cgagggggat
     3961 tttcttgaat ataagaaaat gcagactagt atcgacctcc cacaagaatt aactattccc
     4021 gagcatatct tgatcaatgg aaatcaaatt gatgccaaag atatcttgat aaccggaaat
     4081 cgagtcacgc taaatctgac agattatttt attgctggag atacgttaat aatcgaattg
     4141 gagacaactt tgcaggaagt aattcctgaa aaagtactca ctatgcattt tgaatacctt
     4201 gaaaacggga atttgattga gcaatcgaat gaaataaacc taaagatcgc aaagctgaag
     4261 gagatagata tccatgttta ttataaagat aaagacagtc agattgatat tgctgaaatg
     4321 aaaacaatca ctgggaaaat aggagagaag tataaagaaa ctcctattga tattgaaggg
     4381 tatgtttttg actcggattc agcgaataca gaaggcatct tctccgaaaa tacagaggat
     4441 atctattttt attacagaaa aggcaaactt tatctctcaa ctgctcctca atatgtcaat
     4501 tttggaaaac ataaaatatc cagtagacca ttaagtgaat tcggacagtt ttcaggtggg
     4561 ttgaaaatta tagatgaacg ggcttttgga agttggaaat tacaattgaa acaagagaaa
     4621 gttcttacaa atggagattt tgagcttcca gaagctcttt cctttgttac ctctgaaaat
     4681 acaacggtta tagggacatc cgctgttacg attttcgaaa gtgatcaaaa gggtgaaagc
     4741 gatttgtcct ctcttttaga ttctacagct catcgtggaa tcagaataga tattcctgta
     4801 gaaaaccaaa gaatcggaac ctttgagggg aaactatctt ggatacttgc agatgtccca
     4861 ggaaattaac aggaagagaa gaatataaca aaattattga gtttcaagaa gggcacaggt
     4921 accataggct cttgtgtccc acagagacaa gattttgtta tagtcttctc ttacatatga
     4981 ttaaaggagc gattttcttg agaccaaaac aacaattatt tataacactc ctagttattt
     5041 taggggcact ttttaacaat atatcagtgt cagctagtgc atttaatttt tcagtgacgc
     5101 cgataacttc agagaatcaa atcgacaaga gaaaaactta cttcgatctt caacttgatc
     5161 cggatcagga agtagaagta aaagcggaat tgagaaatga tacagaaaaa gaagtgaaaa
     5221 tcgatatttc tgtgaatagc gcaaccacca attcaaatgt gatggttgaa tacggaaaaa
     5281 acgagattga aaaagacatg agcctgatct ttgatctggt agattatgtg agctatccac
     5341 agatagtgac gctaaaacct aaaagtgtcc aaaccgttgc ttttcatgtc aggatgccga
     5401 atgagcgatt tgatggtgta ttggctggtg ggatcacatt ccaagaaatc cagacggaaa
     5461 aggaccaaac agaaactaag gatcaaagct tatcgattga gaatgaatac acctatatta
     5521 ttgctttgtt gatgcaacag acactgaacg aagttgctcc gaaccttact cttcatgaag
     5581 ttaaaccaga tcaaatcaat gcaagaaatg tcatcttagc aaacgttcaa aatgatcaaa
     5641 aaacctatat caatcaagta gttattgaaa caaaaattac aaaaaaagga cattctgaag
     5701 ttttatatca agaggaaaaa gaaggattgc agattgcgcc gaatacaaat ttttcttttc
     5761 caactgcttt aaatggtcaa ccgctaaccc caggtgaata tcaccttaca atgactgttt
     5821 taggaaatga aaatgaaaat ggaaaatttt cgagaaaaaa aggaaatact acgatcaact
     5881 ataccaatca atgggtattt gaaaaagatt ttacaataga cggaaaagta gccaaggaat
     5941 tgaacacgac ggatgttacg attaaaaaag acaataattg gatttatatc ttagtagctt
     6001 cacttttctt attgtttatt ttattatgca tacttttgtt tgttttgaga aaaaaagaag
     6061 agaaagaaaa gtaaggttaa gataacaaat agtaaatttt tattattgta ggatggttgg
     6121 gctcttttta taacaatata aaatatacca ttttattaat aaaaatgcat actagtaaat
     6181 gatataattc taataacagg tttgatcgcg agaaaggcca aataaaacgc tctccaaaat
     6241 gttaaaaaaa agacaggacg atgtatatta tacaaacaag aatcgctgac aacagtgact
     6301 cggatccagg aaatcagttt tatccccagc tagttggtga atgcatggaa agtaatatac
     6361 gtagaaagag ctgatgacta atgaattatg agtttttttt aagcaaagaa gatcacagaa
     6421 gttattcatt acttaaatat ttagaagcaa gctcaaattt atcagagtca atatcgaatg
     6481 ttcaagaaga gttgtcgcta tcgacatttc tcttgaaaaa aacgatcgat aagctgaaac
     6541 atgatttaca aaagttcgat ctagagaaaa atttgcgagt aaccgtcagt gaattggata
     6601 tatgtctgga aattaatgga aaatgttcta gcaaagctct gttatctcgt tatataacgg
     6661 attctttatc tatgaaaatg gtattggctt tctttcagga aaaatatatc tcagtagaaa
     6721 attttgctaa acagcatcac gtgagttatt ctgtggctta taaagtatta caaagactaa
     6781 aaagaaatct aaaaaaatat caaatttttt ttgaaaagag aaagttg
//
LOCUS       DACEOE010000137         6800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEOE010000137 DACEOE010000000
VERSION     DACEOE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.139"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..325
                     /gene="sgrA"
                     /locus_tag="IW774_002167"
     CDS             <1..325
                     /gene="sgrA"
                     /locus_tag="IW774_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1154501.1"
                     /translation="SSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSV
                     LKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSL
                     FHRVK"
     gene            623..979
                     /locus_tag="IW774_002168"
     CDS             623..979
                     /locus_tag="IW774_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154502.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            982..2139
                     /locus_tag="IW774_002169"
                     /pseudo
     CDS             982..2139
                     /locus_tag="IW774_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2165..2605)
                     /locus_tag="IW774_002170"
     CDS             complement(2165..2605)
                     /locus_tag="IW774_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1154503.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2765..3271
                     /locus_tag="IW774_002171"
     CDS             2765..3271
                     /locus_tag="IW774_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1154504.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3439..5352
                     /locus_tag="IW774_002172"
     CDS             3439..5352
                     /locus_tag="IW774_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1154505.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5357..6358
                     /locus_tag="IW774_002173"
     CDS             5357..6358
                     /locus_tag="IW774_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1154506.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIEKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6560..6766)
                     /locus_tag="IW774_002174"
     CDS             complement(6560..6766)
                     /locus_tag="IW774_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1154507.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2499 a         1026 c         1363 g         1912 t
ORIGIN      
        1 gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa aagtaaataa
       61 tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg cagggagcaa
      121 cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc atcaagaaaa
      181 agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta gaaccttgaa
      241 caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa gctattttcg
      301 ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat tgaataaaac
      361 gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg aagttgtagc
      421 tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa caggaacaca
      481 aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag tatctctgaa
      541 aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa gtctaaataa
      601 tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt
      661 atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat
      721 cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc
      781 gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa
      841 agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac
      901 gattggttat attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct
      961 tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact
     1021 attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa
     1081 gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat
     1141 tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat
     1201 gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg
     1261 caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg
     1321 catccattga taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca
     1381 aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt
     1441 ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa
     1501 agttggggct ggattgaatc gtctattgta gaaaattttt aaaaaaactt caagatttgt
     1561 tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca
     1621 agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat
     1681 tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct
     1741 cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac
     1801 tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa
     1861 tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa
     1921 cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat
     1981 cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa
     2041 cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa
     2101 aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat tgtttatgcc
     2161 aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga ccagaaagca
     2221 agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg attttatctt
     2281 cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt ttcaataaag
     2341 aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct attctgtgaa
     2401 caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg ttgtttgtca
     2461 attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag ttctgttcga
     2521 taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac gagcagccag
     2581 aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt atgaaataat
     2641 aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa atgttgtcac
     2701 gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga aagaaggcac
     2761 aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag gggtatgttt
     2821 tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata aaagaatcaa
     2881 aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg ctcaattagt
     2941 gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc acagttattt
     3001 tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt tattaactga
     3061 attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta tagatacgac
     3121 tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag caacgacaac
     3181 ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga aaatttggga
     3241 taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa aaagcagaat
     3301 ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc ctgttctatt
     3361 tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca tataattctc
     3421 ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa gggagcgttg
     3481 gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg agagagcatt
     3541 tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc tggaaatgaa
     3601 acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga gacatctgtt
     3661 actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga agaagcaaca
     3721 gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa agagaatggg
     3781 acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata tttgaattac
     3841 ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa gtcaaaatta
     3901 atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa agagattatc
     3961 aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg
     4021 atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac aggcttctat
     4081 ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa aatgatggat
     4141 gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca tggcggattt
     4201 cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta taaggccggg
     4261 tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga ttatgtgacc
     4321 tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt tcgagagcgg
     4381 ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat gatgtctgta
     4441 ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg atttgcatca
     4501 attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact accggatgcc
     4561 ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt tatgggcctt
     4621 ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc tctacctacg
     4681 attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga tgatgtagta
     4741 cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga tcttttatta
     4801 gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta cacgaaacat
     4861 gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta tgtggatgaa
     4921 aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc ttatggagaa
     4981 aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg
     5041 actgaaggga tttttaatga gacgatacca actattattt tctattacaa aaaaatccca
     5101 gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg aaagaatgaa
     5161 cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt gaaagtacaa
     5221 aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt
     5281 ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga
     5341 aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat
     5401 cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt
     5461 gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga
     5521 tttccagcaa aaaatcgaac aaatcgatat agaaaaagaa atatcctatc cttcaaagta
     5581 caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct
     5641 tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt
     5701 ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc
     5761 ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa
     5821 agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc
     5881 acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca
     5941 tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt
     6001 ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg
     6061 tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc
     6121 cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa
     6181 tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt
     6241 cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt
     6301 cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag
     6361 tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt
     6421 caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat
     6481 ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct
     6541 tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc
     6601 cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt
     6661 ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa
     6721 tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct
     6781 tttttataat tgtatgataa
//
LOCUS       DACEOE010000138         6774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEOE010000138 DACEOE010000000
VERSION     DACEOE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.140"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..575)
                     /locus_tag="IW774_002175"
     CDS             complement(<1..575)
                     /locus_tag="IW774_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1154508.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVS"
     gene            complement(590..991)
                     /locus_tag="IW774_002176"
     CDS             complement(590..991)
                     /locus_tag="IW774_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1154509.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(969..1445)
                     /gene="ybeY"
                     /locus_tag="IW774_002177"
     CDS             complement(969..1445)
                     /gene="ybeY"
                     /locus_tag="IW774_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1154510.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(1462..3654)
                     /locus_tag="IW774_002178"
     CDS             complement(1462..3654)
                     /locus_tag="IW774_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1154511.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(3678..4649)
                     /locus_tag="IW774_002179"
     CDS             complement(3678..4649)
                     /locus_tag="IW774_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1154512.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            5354..6733
                     /gene="brnQ"
                     /locus_tag="IW774_002180"
     CDS             5354..6733
                     /gene="brnQ"
                     /locus_tag="IW774_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1154513.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         1871 a         1406 c         1247 g         2250 t
ORIGIN      
        1 gaaacgatga agtattcagg atgatcagtc acttgatcat ctggaaaata ttgtgggccc
       61 tctggcattt cactgactaa tacatccatt aagcgttcga cattgtttcc ttctgtagca
      121 gagatcggaa tcacttgtgc aaaaggcatc tgcgtcgaat aatcttcgat gattcccagc
      181 aactcgtctg gatggatctt atcgatttta ttgataatca gataaactgg agaatgcacg
      241 ttttttagac gttcaataat aaaatcgtct ccccggccgc gtttttgatc agcactgatc
      301 ataaataaga ctgcatccac ttctttcaac gcagtgtaag ctgcttctac cataaaatct
      361 cctagacgat gctttggttt atggattcct ggtgtatcga taaaaatcag ctgagcttct
      421 ggtgtagtat atactccttg gattttattt ctagttgtct gtgccttgtc actcataatg
      481 gcaatttttt gacccacgat tcggttcaat aatgtggatt ttcctacatt cgggcggccg
      541 acgattgcga caaaccctga tttatgttct ggcatatacg ttctcctttt taattaaaca
      601 acaaatgata tattttaggg acaaataaaa tcaacccaat gatgacagca aaacaagcgg
      661 tcactaaaac tgctccagct gccatatctt tgattttttt cccaatcgga tgaaaatgaa
      721 aatccgtaaa catgtccact acattttcaa aaacagtatt gatgatctct actatccaaa
      781 cgagaaaaac agctaaaagc agccataacc actctatccg atcaatctgg aaaatcacgc
      841 ccataaataa agcaaggata ccgaacgcaa catgtttcct catattgcgt tcttcctcaa
      901 aaacggtttt gactccttgg acagcaaatt ccacggacat caggaaatgt ttgtttttct
      961 ctacgttttt atcttttgag tccataggca tccaagatct ctttctgtaa accaaacatt
     1021 tctttctcat cttctggtgt catatggtca tacccgttga tatgcaagaa tccatgtacc
     1081 gctaaaaagc caagttcccg atcatatgaa tggccgtaag cttctgcttg ttcagcagca
     1141 cgttcaatag agatcatgat gtctcctagt acacgaggca atggattctc tccatcttca
     1201 aaaatcactg ggatttcgtc ttcgccttct tcttccagag caaaactgat gacgtctgtc
     1261 ggtgcatctt ttccacggta atcacgattg attacttgga ttgccgcatt gtccatgaaa
     1321 gtaacagaca tttctgtttc ctctggcagc tttaaataag atgctgcaaa ttgcagcaag
     1381 tcatcgattt ctttcatttt ctcttctgag acttggtttg tttcatccat aaaggtaata
     1441 tccattatac atcgcttctt tctattcttc ttgttcttcc tttatcttct caacttctgt
     1501 tttcatttta ggatatttga tccgagaatg gaaggtgcta cttaaaccgc tgatcaatga
     1561 tttttccact tttcggattt ctttcaaggt caatccacta tcgtctaatt gaccatcgga
     1621 aatacgctct tcgataagat tatggacaaa actttcgatt ttttcgctcg ttggatgatc
     1681 cattgcccgt actgcagcct cacaactgtc agcaatattg acgatccctg cttcacgact
     1741 ttgcggtttt ggtccaggat aacggaattg ctcttctgtt acttccggat tgcgttcttt
     1801 cgctttgatg tagaaataac gcattagcgt ggttccatga tgctgtttgc aaatatcgat
     1861 caccatttgc ggcatattat aatcttccaa tattttggct ccatctgtca catgtccaaa
     1921 gataatatgt ttactgtctt ctggcaataa gaaattgtgc gggttttccg ctcctgaagg
     1981 caagttctca acgaaaaagc tagcgtgttt gatcttgccg atatcatgat aatagcaagc
     2041 tactcgagtt agtaaagaac gtccgccgat ttctgctaca gcgttagcac ttagattagc
     2101 taccatcata ctatgatgat acgtacctgg cgcttcttcc agcaactgtt tcaataaagg
     2161 atgatttgga ttgctgagtt catttaatac aattacactg tcatcattga ccatcaattc
     2221 aatatatggg tgtagaccga tcgtcagcaa gaatgagaag agtgcaccag tgaacccgca
     2281 gacaagcatt aaccaagtcg ttgcatcacc aaagctcatt ccttgataga cgatcaaaat
     2341 gacatccatc atcacaggaa aaataatgac ccacatggca gcagataatc cttgttctga
     2401 gatccgttta cgtctgacaa tcgttgccat gagcccagag aaaacataag acatcaagat
     2461 aatcgttaga aagttcgtgc caatcgaatc ataaaaaatg aaaagtgcgg atactgcttg
     2521 gaaaatacca gccatgatcc ctgctcttcg attcaagaaa aagcttaaaa caagtggtgc
     2581 aaaagcagcg ggataaaaca gcgggatata agtagcttgt tccgtttgga ataattggaa
     2641 aaatttcatc agcactatgc tgactgacat agctgtcaca tagaaaagaa cgtattttgt
     2701 tctttctcct gtattttgga attgaagtga attataaatc agtacgccga tttgaagcaa
     2761 aacagctaaa accatggcaa ttagcggaaa aatcgaagtt gtttgattag tcagtcctag
     2821 taattccaat tttttcatag ctgaagcatc aatttgatta ccttcacgaa caatgatttc
     2881 tccttggaag atcatcactg gttgtacagc ttctcgtgcg gattgtttca attcttctgt
     2941 ctttttctca ttcaatacgt cattcactac gataccttga tccactaagt agcgaatcat
     3001 ttgttgttgt gctggtgtga cattcaaata ttggatttgc tcttctgctt cttgtttaaa
     3061 agcttccaat tcactttcac gaacttgttt gctcatctgt tcgtcaatga gcttcagact
     3121 ctcatccctc acagtatgaa tctcagtttc agtcatttca aatatcgtcg aataaaaatt
     3181 gttcggtagt ttttgataaa acacaacatt ttctgcattc acattttcaa aatttttctt
     3241 cagcgcggcg atccgctctt ctgtagtagg ctttgggata ttttcatctt cctttgcctt
     3301 atcaatcttt tcgtcataag atttgttgat ggcatcattc gtcttatcga tcaattcaaa
     3361 tagttgcttg atccgattgt tctggtcatc tgcaagatct ttttggtaag tgtattctgg
     3421 agtgactgct tctgctgcca gttttctttt ttgttcggtt tcttccgtgt tctcgatcgt
     3481 tttgttcgca cggatacttt cttctgctac ttggccttct ctgaaatcat tggccttttg
     3541 tttgacacta ctaaatgtaa cgccgcagat aatgataaaa aacaagaaca gcaggaaagg
     3601 caatgctgcc tttccaagtt tttcttgtag tttctgcagt gttttattca acatgtgctt
     3661 atcctcctaa gcaaagctta ttcttgatgg agatctgcct tttcataagc tttgatgatt
     3721 tcggcaacta cagggtgccg aacaacgtca cttgcttcaa aattaacaaa atcgattttc
     3781 ttgatttgct ttaatgttcg ttctgcatga acaagaccac ttgttgtccc tcgaggcaga
     3841 tcaatctgac tcgtatctcc attgacaatc atttttgatt gataaccaag acgtgtcagg
     3901 aacattttca tctgagcaac tgtcgtgttc tgtgcttcat ccaaaataac gaatgcatca
     3961 tccaatgtcc gcccccgcat gtaagctaaa ggagcaattt cgatcacgcc tcgttccatc
     4021 aatcggttcg tatgatctaa tccaaaaatt tggtataaag catcataaac aggacgcaga
     4081 taaggatcta ctttttcttt tagatccccg ggaagaaagc ctagattttc ccctgcttcc
     4141 acagcaggcc gcgtcaaaat gattttttgc acttcacctt ttttcaaggc agcgattgct
     4201 aatactactg caagaaaagt ttttcctgtc ccggctggtc ctacgccaaa tacgatatca
     4261 tgtttggcta ccgattcgac atatttcttt tggccgctgt tttttacccg tataggtttt
     4321 ccattacggt ctttgacgat ctcttcctca tacatctcaa caaaataatc taatgtgcct
     4381 ttttgcccca ttttcagtgc cgtcacgaca tctggtgtac ttacatggat tcctcgtttg
     4441 atcaattcct gcaatgtccg aatgactgag gcagccaagg aaatctggga ttgttcgcca
     4501 atgatctgaa tgatttctcc acgtgtatgg atggttgttt ctgttgtttc ttcaatcagt
     4561 tttatgtgct tgtcgtgtgc acctaaaagc atgctgacat catcagcagc cgtcaatcta
     4621 atctctaaag aactagactc ttccgtcaaa aatagacatc cccttttttt cgattcattc
     4681 tttcctattt taccatactc agaagaaact atatagcaaa taagaaaagg ttctaaaaga
     4741 gttgcttaac cataagtacc aagaaacgtt aaaaaatagc tgcttccatt ctctaaaggt
     4801 ctcgtttgaa taaaaaaagc tgatcatgta agctatgcat aagatagagg acaagtattc
     4861 cgtctaactt agcaagtatt ctctgggtat ttttcttgtt tcaaaaaaag aaaacaaaat
     4921 atttgaaata ttcttgcatt tcaaaaaaaa caggtgtatt ataacgtcaa caaataaata
     4981 tacagcaaag cgtcgaggaa aacagtaggc tattcagtga tttattccca gtgagtcatg
     5041 taagtgagaa atgacaataa atcgaataga cgaacatcat ttccgagtca atgtcgaaaa
     5101 cgtagatcat tgtggctgcc tccattatca ggccagcgga taactttatc gaaaagcgtc
     5161 cgtgaatgaa gaagcttttt taagcgaaaa aaggtggtac cgttctagaa tctagagccc
     5221 ttttcccaag tggaaaaggg ctttttttat attttaaggt ggtggttttc atggaatctg
     5281 atattagtgg tatttctagt ttctgcaacg tttattttgt tactcaaaat gataattatt
     5341 ggaggaaaaa tttatggaag aaacaaaaac ttattcatgg aagcaattat tgatggtcag
     5401 ttcacttatc ttcgggatgt tcttcgggtc aggtaatctg atctttcctg tccacttagg
     5461 tcaaatggct ggatcaaact ggatcagcgc tggtgtagga tttgccatct caggcgctct
     5521 tttcccatta ttagctatct tagctgtagt agtgacaaaa agtgatggtc tgtatgactt
     5581 agctaaacct gtcggaaaat ggtatgctgc cttgttcttg atccttgttc atttaacgat
     5641 cggaccattc tttggtacac cgcggacagc agcaactgct tttgaaatgg ctgctaaacc
     5701 ctttttgcct gaaaaatatg attcattagg tatgttttta ttctctgctc ttttcttttt
     5761 acttgcttat ttccttactg tacatccaac acgtttggtc aaatatgtcg gaaagtattt
     5821 aaactccttg tttcttgcat tattagctat catttttgtc gtttctttat tccaacccat
     5881 ggggaatcta aaccaatctg ctgcagattc ttatcagacg aacgctttga caaatggcgt
     5941 tttagaagga tacaacacgg ttgatgcagt agctttactt gctttaagtg tcacttttgt
     6001 ccatgcagtt cgtgatcttg gttttaaaga taaaaaagtc acagaattaa cagcaaaagc
     6061 tggtagcctg agcgttgcgt tagaagtcat gatttatttt ggtctggtcc ttctcggtgc
     6121 gatgagtttg aataagatgt ctttatcaga aaatggcgga actgcacttt ctcagatcac
     6181 taatttttat ttaggtaact tcggtgctag cttcttaggg gtaatggtca cattaggtgt
     6241 cttcactacc gctatgggac tcgttgtttc ttttgcacaa gatttccata aactgttccc
     6301 taaagtcagt tatatgacat ggctgcggtt aacgactttt gtctcttttg tcgttgcgaa
     6361 tgccggattg gataatatca tccaatggtc tttaccagta ttgatgcttt tgtatccatt
     6421 gtcacttgcg ttaatcttgc tttccttaac agcaaaattc ttccaaaaga gtccatttgt
     6481 ctatcaagta acgatgttct tttctgccgt tccagccgta ttggatatgt tggcaaattc
     6541 gcctgcatta gtgagtcagc aaactgtcgt agcaagctta ctaggatttt atcatcatta
     6601 tgttcctttt gctgcattag gacttggctg ggtcgtccca acgttgcttg gttacgctgg
     6661 cagcttgctc ttttattatg tatatcgcgt atcaggatat aaacaagaag caagtgagtt
     6721 accagaagaa taaccaaatc aaacacaatt taacataaga gatattaaac agga
//
LOCUS       DACEOE010000139         6769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEOE010000139 DACEOE010000000
VERSION     DACEOE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.141"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            58..537
                     /locus_tag="IW774_002181"
     CDS             58..537
                     /locus_tag="IW774_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1154514.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            756..1436
                     /locus_tag="IW774_002182"
     CDS             756..1436
                     /locus_tag="IW774_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1154515.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            1444..2775
                     /locus_tag="IW774_002183"
     CDS             1444..2775
                     /locus_tag="IW774_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1154516.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            2790..3752
                     /locus_tag="IW774_002184"
     CDS             2790..3752
                     /locus_tag="IW774_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1154517.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            3863..4201
                     /locus_tag="IW774_002185"
     CDS             3863..4201
                     /locus_tag="IW774_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1154518.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            4280..4987
                     /locus_tag="IW774_002186"
     CDS             4280..4987
                     /locus_tag="IW774_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1154519.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(5090..6571)
                     /locus_tag="IW774_002187"
     CDS             complement(5090..6571)
                     /locus_tag="IW774_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1154520.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2200 a         1116 c         1437 g         2016 t
ORIGIN      
        1 ttttgaatac agcatccctc gctttataat aaacgataga ataaagggag tgaatcaatg
       61 atgagtcaaa caaaagtcga acgttattta gaagaacaaa agatcagcta tcaaccactt
      121 gattttagtc atgtggtaga agaatctttc acagaagaac tacaaaaaag agggattgat
      181 ccagctttga tatgtaaaac tctcgtagtg aaaggaaaca aaactggggt gatcatcgca
      241 ttgattcctt tgaacgatca cttagattac aaaaaaacga gacaagtgac gaaagatcgt
      301 aaagttggtt ttcctggaat ggattttgta ttaatgcata caggatatcc ccacggagca
      361 aacaccccta ttggaataaa actcgcacat cctgattatt gttttttagc agacagttct
      421 ttaaaagaaa aagaagaagt aattgtgtca agcggtgaaa tcgggagaag ccttcggata
      481 aaagtagccg atttagaaaa gatcgtccat cctatttacg ctgatttaat tagttaaggg
      541 acgagaagag aagctaagca gagcagagac ctcaaacttg agattatatc tgcttggtac
      601 ttggtgcatc ctgtattatc tgttacaatt gctttgtaca tactgtacta tcaatccaat
      661 gaaaatatat ttagcagcta tattttttca ttgatgtgca agtgatgaaa tcgtttttaa
      721 taaaaaagat acagagaaaa cgcgaggtgt aaaatatgaa aattacgtat catggacatt
      781 cctgtttaac aattgaaatg gaaaatggcc aacgcgtgat gattgacccg tttattacag
      841 gaaatccatt gacagactta actgcagaag aagtaaagac agattggttg ctgattactc
      901 atggtcatag tgaccacata ggagatatga ttcctattgc aaaagaaaat caagccacaa
      961 ttatcagtat tgttgagatt gctgattatg cgaacagcag aggagtagat tcattcggta
     1021 tgaatatcgg tggaaaacat gcgtttccat tcggcgtggt aaaattcgta catgcacagc
     1081 atagttcaag ctatgaagta gacggtatcg ttcagtatat gggcgagcct agcggaatta
     1141 ttattcaagc agaaggaaaa acgatttatc atgcagggga tactgcttat ttcagtgatc
     1201 taggattgct tgcagaagaa ttcgatattg atgtcgcttt cttgccgatt ggcgataact
     1261 atacgatggg gccagaagat gcagcaaagg ctgcgcaaac aattcgtgcc aagaaagtag
     1321 ttccaatcca ttacaatact ttccctgtca ttgaacaaga tcccgaatct tttgtagagt
     1381 tactggaaga tagtgaagga aaaataatga gcgtctcgga aacgctggaa gtgtaggcag
     1441 aaaatggcga caaaacatga tttgatttta gaatatattg aaagtttacc tgttggcaat
     1501 cgaatttctg taagaagcat cgcaaaagaa ttgaatgtca gtgaaggaac agcgtaccgt
     1561 gcaatcaaag atgcagaaaa tgtcggacta gtttcaacaa ttcaacgagt agggaccatc
     1621 cgtatcgaac gcaaattaaa aaaacatatt gaacgtttga catttggcga ggtcgtcagg
     1681 atcattgaag gggacgtttt aggcggggcg gcaggattag acaaggtctt gaataaattc
     1741 gtcatcggcg cgatgactaa acatgccatg gaacgctata ttacgccagg atcactaatg
     1801 atcgtcggca accgggcgga agtccaaaaa ttagcattgg aagatggcgc cgcagtattg
     1861 atcacaggag gattcaatac gactgctgag attgcaaagc tagctgatga tcttgaaatg
     1921 ccgatcttac gcacctctta tgatacattt acagtcgcaa ccatgatcaa ccgagcgtta
     1981 agcgaccaat tgatcaaaaa agatatcatg cttgtcagtg atatctatat gccagctgaa
     2041 aagactcatt atttgcagca gacagatacg atttctgatt atcagcgttt gtcagaagaa
     2101 acgcagcatt cgcgttttcc agtcgtcaac cgccatcatc gtttgatggg gattgttacg
     2161 gcaaaagatg ttttagggaa atcgccaaac cagatcatcg atcgggtgat gacgaaagaa
     2221 ccaatcagtg taaagaaaac aatgagtatt gcctctgtta gccatcaaat gatctgggat
     2281 gggctagaag tcatgccggt cgtttccgat gacttgacat tggaaggctt gattacccgg
     2341 caagatgtaa tgaaagcgat gcagcttgtc cagcgacaac cgcagattgc agatacgatt
     2401 tcggatcaga tatctggaaa tatccatacg attgatactg accgtgaagg aaatcgtttg
     2461 gaaaacccta agttcaaatt cgtagtagca ccacaaatgg tcaatggcgt tggaaccatt
     2521 tcttttggcg tacttagcga aatcatttct gatgtcgcac aaaaaacaat ggtgatgaac
     2581 caaaaacgaa atatcttgat tgaacaagtg aacctacatt atttgcggct gatccaattg
     2641 gaaagcgaat tagacattcg tccacgtgta ttggaaatcg gcagacgttc cgctaaactg
     2701 gatatcgaag tgtttattga aaacaccatc gtttccaaag caatcgttgt ttgtcaagta
     2761 atggaacgtt cttagaaagc aggagtgaaa tgacagtaca gcaagaaata gtagatacaa
     2821 taaaaaaata cgataaaatc attatccaca ggcatttgcg ccctgatcct gatgcattag
     2881 gatcacaagt aggattagcc gaactattac gggaaagttt cccagaaaaa gaaatacgtc
     2941 aagtaggtga aacgattgaa gggcttcgct ttttagcaga aatgcaagaa gttgaagatg
     3001 tcttctacca aggagcgcta gtcatcgtga ctgatacagc taattcaccc agaatcagtg
     3061 atcaaagata tcagttggca gaaaaactga tcaaaatcga ccatcatccc aatgatgacc
     3121 cttatggaga tcttgtgtgg gtaaatacaa aagcaagtag ctgtagtgag atgattgcag
     3181 aatttgcttt gatgtttcct gaatctttaa agatgaacgt taaggctgca cggttacttt
     3241 atgcaggaat cgtcggagat acagggcgct ttttgtatcc ctccacgacg tcccgtacat
     3301 tagagattgc ttcgatattg agaaattatc catttgatgc aagtgctttg aacagagaga
     3361 ttgaacaaat gccgatgaaa gtcgctaaac tttccggtta tctttatcaa aatatccagg
     3421 tggatgaaaa tggtgcagga agaataatcc ttccgcaaga aattcttgat aaatatggaa
     3481 tcgatgattc agagacagct tcgatcgttt cacttcctgg agtgattgac gaagtgctgg
     3541 cttgggggat ttttgttcag cagccttcag ggtattaccg cgtacgttta cgttctaagg
     3601 ggccgatcat caatgaattg gctaaacgcc atcatggagg aggccatcca ttagcaagtg
     3661 gtgcgaatgc aaaagattta gcagaagtag aggaaatcta taaagagatc caagatatat
     3721 gccaatcgta taagagcaga aaaacagaat gatacttcgt tcgaaaacgt gccttttgta
     3781 gtaaaaatca caaatgtatc ttttcaagat cttctttttc cgttacaata gtgctcacta
     3841 aagattttaa aggagaaaga agatgacatt accaaattgc ccagaatgtg gctcagagta
     3901 cacatatgaa gatcgaggct tgttgatttg cccagaatgc gggaacgaat ggaacaaaga
     3961 agaacaagaa actgtcatag aagacggatt agtggtatta gatgcaaatg gaaaccgtct
     4021 gttagagggc gatagcgtga cagtgattaa agacttgaaa gtaaaaggtg cgtcaggtgc
     4081 cattaaacaa ggaacaaaag tcaagaacat ccgtttagta gaaggcgatc ataatatcga
     4141 ttgcaaggta gaaggatttg gaccgatgaa actgaaatcg gaatttgtga aaaagaatta
     4201 aagaatattc aaggtctttt cacattcttt tcctactact aatgataaaa taaagtaaac
     4261 gaaaaggagt gaaacgaaaa tgtatggtta tggaatgtat ggttttttag atccgacatt
     4321 tcttttggtc attcttggat tagttatttc cggaattgcg tcagcgtacg tcaatagtac
     4381 tttccgaaaa tacgatcaag tgagaagcaa aaacaatgtg actggtacac aagcagcaca
     4441 atatatatta caaagtcaac agataaataa tgtcggtgtt caacaaattg ccggagatct
     4501 cacagataac tacaattcag gtaataagat gttgagtttg tctcaagcaa ctgcacagtc
     4561 tacttcagtt gctgcgatag gtgtagcagc tcatgaatgc ggacatgcgg tacaagatgc
     4621 ttcgggttat gtcccattga agattcgtgc agcacttgtt ccagcagcta actttggttc
     4681 gatgatttcg ttccccttga ttatggtagg tgtattgctt agctggaact ctacgttaat
     4741 caatatcggg attttcgctt tttctttagc attacttttc caattggtga cattaccagt
     4801 cgaatttaac gcgtctcggc gagcaatcca aattttgagt gaaggcggac tactgacgga
     4861 agaagaagta ccgatggcaa gacacgtatt atttgcagca gcactgactt atgttgcagc
     4921 agcgctttcg acattcctgc agctgttgcg cttgatctta ttgttcggcg gcaaccgaag
     4981 agattaaaat aaagaagttg tgaaatcagc gctttgatct gagctaatac cgagagttag
     5041 ctgattgaat accatttatt tggaataaga ggatgtgaca agaattttgt cacatcctct
     5101 ttgttttgtt ctcataacct tttttcatat acagttcatg aaccgctttt attaacttgc
     5161 caaataacac tgtattgtgc gttgtatcca gatagaaagt ctccgtttca tctgaaattt
     5221 tctcatgctg atctgtaatg atcaaatcag catgttctga atatgcggta attttaacag
     5281 catctttgcg aaataactgc tgcaaatggc tttgaagatg ataatttcct gtaaactgtt
     5341 tggttatctg caaatggatg agtaactgct ctttcgtttc tgtttgtagt aagtgttgga
     5401 tcaattgact aaggtattga ctgatttggt ttctttcccc attttttgaa aaaattgtat
     5461 cgattttttc tcttaatttc ttttcctgta taaaagaact agaatgattg gacgtggctt
     5521 ctggaaagca aagtgtagaa aaactactgc tggattcacc caacagttcg aaaaaaacaa
     5581 ctgccaacac aagaaaataa aaataaattg gtattttatt ttctaaatgg ttgagaggaa
     5641 actgttgagt taagtaagtc atcaattgac cgacttgtcg acaaataagg tttttgcttg
     5701 tggaaaataa aaaaccaatc tcctgttttt gtcgaaaaga gatttgattt ggaaaaaaac
     5761 aatagaaaaa ataattaaag taggctctct ctgtttctgt ctcttcacaa gggcgactca
     5821 ctgaaaaaga agtaaagtat tctgaataat catgtgtatg ctgcaataag tccatcaatt
     5881 cagtgatttc agaagaagtt tttggaaaat atgcctgttg tttgtttcgt aatatgaata
     5941 aagttaacag caagtagata tttttttctt gttttgaaat tttttctatt tgctcatctg
     6001 tactgttcat ttttagtgca tttaattcaa gtgaggaaaa tggccaatcg ttacctccaa
     6061 atgtatctga aagaaaaaag taaagataga gacggataga taactcattt ccttcaagag
     6121 tacagcaatt ttttacagat aggatccgta aatggaattt ttttaaggaa tgattgagct
     6181 gttttgtcag tttttcgaga taggagggcg atatataaag ttgttcaagt aaatattttt
     6241 ttggtacagc agtttgtgga taattgatta gtaacatcaa atagttaaaa cgattggatt
     6301 cttttaggta gcataatttt aacttagaaa aaacagctaa ctttttagtt tgatcggttt
     6361 ctttggtttg gatctctcct ttatgattag aataaataaa ttcatcaatc aataaaaatt
     6421 gcttgagtga tacatttatt tttcggatca gttctttgat tgtccgtttt ggaatatgta
     6481 agtcattgga taattcttgt aaagaacaat tatttttttg aatgaccatt tgtagaacaa
     6541 gtaactcttt ttttcttgct tcaattagca taaatacctc ctttttattc tgtttttgta
     6601 tatagttaga caaaaactgt gagtggaaag tcatttcagt ctgtgttatt ctcacagagt
     6661 gtcgccttct tttttatata acgttattta atttttttgt taccttgttt attctattgt
     6721 ttatttccta gggtattttg tcaagatatt cttgttttta aggcggaaa
//
LOCUS       DACEOE010000140         6693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEOE010000140 DACEOE010000000
VERSION     DACEOE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.143"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            45..677
                     /locus_tag="IW774_002188"
     CDS             45..677
                     /locus_tag="IW774_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1154521.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(679..1584)
                     /locus_tag="IW774_002189"
     CDS             complement(679..1584)
                     /locus_tag="IW774_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1154522.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1669..2322)
                     /locus_tag="IW774_002190"
     CDS             complement(1669..2322)
                     /locus_tag="IW774_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1154523.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            2644..3588
                     /gene="mvk"
                     /locus_tag="IW774_002191"
     CDS             2644..3588
                     /gene="mvk"
                     /locus_tag="IW774_002191"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1154524.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3581..4558
                     /gene="mvaD"
                     /locus_tag="IW774_002192"
     CDS             3581..4558
                     /gene="mvaD"
                     /locus_tag="IW774_002192"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1154525.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4571..5656
                     /locus_tag="IW774_002193"
     CDS             4571..5656
                     /locus_tag="IW774_002193"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1154526.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5653..>6693
                     /locus_tag="IW774_002194"
     CDS             5653..>6693
                     /locus_tag="IW774_002194"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1154527.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRT"
BASE COUNT         2057 a         1369 c         1341 g         1926 t
ORIGIN      
        1 ttcccgcttc tttgctatac taaagaaaaa cgaggtgaac ccacatgaaa aaaatcgaac
       61 ctattccacc ttctcttcct cttcttacaa agggagagtt ttatttagaa gatgaggaga
      121 tcttctctgg tcttctgatc aacgggggtg accaaagcta tctcgatgcc aaaaatatgg
      181 tattaagaga gagtcatatc caaaaactta cgatgcaaaa aacagtttta cgtcattttg
      241 aatgcagtaa tgtcattttt gaaaaatgtg atttttccaa tctggagtgg attggtggca
      301 gtttccacca agtcgtgttt catcaatgta aattaacagg tacgaatttt gccgaaagtt
      361 atcttcgaga ctgtactttc actgattgta ttgcttcgat ggcttccttt agtagtacaa
      421 acttgaaagc tgtctccttc gatcattgtc aattagaaga cagcgaattt tatgaggtca
      481 cttggaaaaa cctttttttg tctgacaatc agttgaccgg ttcgaactgg tttcgaactt
      541 ctctgaaggg attagacttt acgactaatt tcttcacagc aatcgcttta tctcaagatt
      601 ctttggcggg actaatcgtc aaccaagagc aagcacttgt gatcgcccaa gcccttggtt
      661 tgcagatcaa agactagatc aacgaatccg ccgccacagt gcaataagca tccaaataga
      721 taagaccaaa ctgatgatca aggcgattcc ccatccgaaa acggaatggg taaagggtaa
      781 ctggagattc attccgaaga atcctgtcac aatagttgga acggtcaaga tcagcgacca
      841 gaccgtcaaa aatttcatcg tgtcattcaa gttgttattc agcaaattat tataagtgcc
      901 agagagctgg tctaaaatct gcgaagctaa gttcgtcatt tcaactgctt gtcgtgcttc
      961 gatcagcgca tcgtccaact cttctttttc cttttctgac aacttgcgat aaatagccaa
     1021 tgccttgatt tgttctaaaa gtactacatt ttgttttgtt cctgtaacta agtaaaccaa
     1081 accgatctct aaatctgaaa gttgcagcag attcttgttc gttgtttttt ctcttaattt
     1141 cagattaagc cgctgacgtt cactattcac ttcctcgatt aaaggaaaaa aggaatcaga
     1201 aatcaaaaac aatgtacgaa acaataaact gtatacactc atgtctggca ttcgttccaa
     1261 aagcgatcga attagtcgaa tcacgtactc ggtattcggc gtggtaaatg taaacaaacc
     1321 gtcttctttt aaaataaaag cgataggact agtttcataa tgattatcga ctttttgctg
     1381 gtgcggaaca tttgatacaa gcagtaacgc ttcatcaaaa gaatcgtact ctacgcgcgc
     1441 ccgttcattt ttatctagag aataagtcag catctcgtca ctaatgccat attcttcttt
     1501 taagttttta acagctagca catcgttttg ctgtacattg atccaaaaat tacggtcttg
     1561 attaaaatag tgtttttccc gcatactcta tcctcctgac atttgtttcc agtataaaga
     1621 aaaaagtcgc tcaaataaag gtttaccctt tatttgagca actgtaaatc aaatggctac
     1681 tttggtttgg cgaatctctg ctaatttttc ttcgatcatt ttcaagactt cttcttttgc
     1741 ttgaggatct tccacaaaat tatagcggtc tccatcgatt tggatttttg gactttcttt
     1801 gtattcttca taccattgat cgtaacgtct gttcaattct ttgtaataat cgtatagact
     1861 tggatcaaag gacaattgtt catacgaacg accacgtttt tcgatacgct ccaacatcgt
     1921 atcaaaagat acacgaatat gaactaataa atccggatgt tttttatggg cagcataggg
     1981 aagttcttcc atcatgtttt ccagcagttc atcatacaca cgaacttctg tttctgttgc
     2041 tcttcctaga tctgcattta gatggaaaag cagtgaatct tcataaatcg aacggtccaa
     2101 tacattattg tcatctttca ttgcttgttt gatgcttgca aaacgtttat tcaaaaaata
     2161 aatttgcagc aaaaaagcat actgttcagg gtttgcataa aataagggca agacttcatt
     2221 atcatcaatc gattcataaa atgcctgact atcaaagtgt tcagccatca tttctgttaa
     2281 acttgatttt ccggcaccaa ttgtacctgc taaaacgatc acgctcattt acctccagct
     2341 cacttcatca ctaaatattg tgtttaactc caaccttttt aaatataccc ccaatatcta
     2401 gtagtgtcaa ttgtattttt ttctagtcaa agaagaaaaa aagtcaaaaa attcttaaga
     2461 aaaaagtgtt ttttctttag aaaacgtatt cttagtttta cttctgagat ggttttagta
     2521 aaataaagga ggaatgtttt ttgagaccct tagtggacaa aaaaattcgt gaagcagcgc
     2581 aactcgtttt gggcgcttta ttttttgctt aaaaatcact tttacgtgag aaggagttag
     2641 attatggcaa actatggcca aggagaatca agcggaaaga tcatattgat gggcgagcac
     2701 gcggttgttt atggagaacc agcgattgcc tttcctttct atgcaacaaa agtcaccgca
     2761 ttccttgaag agctggatgc aatggacgat caactggttt cttcctacta ttcaggaaat
     2821 ttagccgaag ctcctcatgc attaaaaaat atcaaaaaat tattcattca cttaaaaaaa
     2881 cagcatgaca tccaaaaaaa cttgcaactg accattgaaa gcacgattcc tgctgaacgt
     2941 ggaatgggat caagcgctgc agtcgccaca gcagtcactc gtgcttttta tgattactta
     3001 gcatttcctt tgtctcgtga aatactatta gaaaatgtcc agctttcgga aaaaatcgcc
     3061 cacggtaatc ctagtggaat cgatgcagcc gctactagca gcttgcagcc gatttatttt
     3121 acaaaagggc atcctttcga ctacttttct ttgaacatcg atgctttttt gattgtcgct
     3181 gatacaggaa tcaaaggaca aacaagagaa gccgtcaaag atgtcgctca cctctttgaa
     3241 acccagcctc atgaaactgg acaaatgatc caaaaattag gatacttgac gaagcaagca
     3301 aaacaagcaa tcattgaaaa ttcaccagaa acgttggcac agacaatgga tgaatcacaa
     3361 tcacttctgg aaaagctgac aatcagcaat gattttctta atctattgat ccaaacagca
     3421 aaagataccg gggcattggg tgccaaatta actggcggtg gacgcggtgg ctgtatgatt
     3481 gcattagcac aaacaaaaac aaaagcccaa gaaatcagtc aagcacttga agatgcgggt
     3541 gctgctgaaa cttggataca aggattagga gtacatacct atgtttaaag gcaaagcacg
     3601 cgcatatacc aacattgctc taatcaaata ttggggtaag aaaaatgaag aactcatcct
     3661 tccaatgaac aacagccttt cgttaacatt ggatgcattt tatacagaga cagaagtcat
     3721 cttttctgat agctatatgg tagatgaatt ttatctagat ggcaccttgc aagacgaaaa
     3781 agcaacaaaa aaagtcagtc agtttcttga cctttttcgt aaagaagccg gcctctcact
     3841 aaaagcttca gtaatcagtc agaatttcgt tcctaccgca gctggattag cctcctctgc
     3901 cagcgggcta gctgctttag caggcgcttg caatactgct cttaagcttg gattagatga
     3961 tctctctctt tcaagatttg ctcgacgcgg gtctggttca gcttgccgaa gtattttcgg
     4021 tggtttcgtc gaatgggaaa aaggccatga cgacttaagt tcttacgcta agccagtccc
     4081 ttccgattct ttcgaagacg atttagcaat ggttttcgtt ttgatcaacg accagaaaaa
     4141 agaagtgtcc agcagaaatg ggatgcgtcg gacagtcgaa acatccagtt tttatcaagg
     4201 ctggttagat tccgttgaag gggatctata tcaattgaaa caagcaatca aaacaaaaga
     4261 tttccaactt ctcggagaaa cgatggaaag aaacggacta aaaatgcatg gaacgacttt
     4321 agctgcccag cctccattca cttactggtc tcctgacagc ttaaaagcga tggatgcggt
     4381 gcgacaattg agaaagcaag gcattccatg ctactttacg atggatgctg gccctaatgt
     4441 caaagtttta gtggaaaaca gccatttatc tgaggtacaa gaaacattta ccaaattatt
     4501 tagcaaagaa caagtaatta ctgcacatgc tggtccagga attgcgatta ttgaataaag
     4561 ggagattttt atgattgaag tatctgcacc aggcaaactc tatattgccg gagaatatgc
     4621 agttgttgaa acaggccatc cagcagttat cgctgcagtc gatcaattcg tgacagtcac
     4681 tgtagaatct gcacgaaaag tcggaagtat ccaatctgca caatatagtg ggatgcctgt
     4741 acgttggaca agacgcaacg gagaattagt tttagatatt cgggaaaatc cttttcatta
     4801 tatccttgct gctattcgct tgactgaaaa gtatgcacaa gaaaaaaaca ttcttttatc
     4861 cttttatgat ctgaaagtaa caagtgagtt agacagttca aatggccgga aatatggatt
     4921 gggttctagc ggtgccgtga ccgttgcaac ggtcaaagcc ttgaatgttt tttacgcgtt
     4981 gaatttatct cagttggaga ttttcaagat tgcagcacta gccaatttag cagttcaaga
     5041 taatggttcc tgcggcgaca tcgctgctag ctgttatggt ggctggatcg ctttctcaac
     5101 cttcgaccat ccttggctcc aagaacaaga aactcagcat tctatcagtg agttacttgc
     5161 cctggattgg ccaggtctat ccattgagcc attgattgct cctgaagatt tacgtttatt
     5221 gattggttgg acgggtagcc ctgcctctac ttctgatttg gtcgatcaag ttcaccgttc
     5281 gagagaagat aaaatggtgg cttatcagct tttcttaaaa aatagtacag aatgtgtcaa
     5341 cgaaatgatc aaagggttta aagaaaataa tgtaacgttg atccaacaga tgattcgaaa
     5401 aaaccgacaa ttactgcatg atttatctgc aatcactggg gtcgtcatcg aaacgcctgc
     5461 tttgaacaaa ttgtgtaatt tagctgaaca gtatgaagga gccgcaaaat cttctggtgc
     5521 aggtgggggc gattgcggaa tcgtaattgt tgaccagaaa tctggcattc ttcctttaat
     5581 gagtgcatgg gaaaaagcag aaatcactcc actgccgtta catgtctata gcgatcaaag
     5641 aaaggaaaac cgatgaatcg aaaagacgaa catgtttcac tagctaaagc ctttcatgat
     5701 aaacaaaaaa acgaatttga cttcgtacgg atcatccata accctttgcc gcaaatagca
     5761 gtttctgatg tcgatctaag tacacaggca gttgggttta cactaagcag cccattttat
     5821 atcaatgcga tgactggcgg aagcgaaaaa acaaaaaaaa tcaaccaaga tcttgcaatc
     5881 gtcgcaagag aagcagattt gatgattgcc actggttcag tcagcgctgc cttgaaagtc
     5941 ccttctttag ccgatactta tacgattatg cgacaagaat atcctcacgg gaaaatcatt
     6001 gccaacatcg gtgcagggac ttctgtcgaa agagccaaag aagctatccg attgtttcac
     6061 gcagatgcct tacagattca tttgaatgca cctcaagagt tggttatgcc tgagggagac
     6121 cgtgatttta ccaattggaa agtacttatt caagaaactc aaacagccat tgacgtcccc
     6181 ctcatcgtaa aagaagtcgg ttttggcatg acaagagaaa cgctcaatga tctagctgct
     6241 ttaggagttc atacagttga tatcagcggt cgaagcggaa caagcttcac ccagatcgaa
     6301 aacgcccgtc gctccaagcg ggaactgagt tatttagctg actgggggca atcgacagtc
     6361 tcttcattgc ttgaagcaaa tgaagcagac acttctatgg aaatcctagc ctccggaggt
     6421 atccgcaatg cctatgacat atttaaagcc ctttgtctcg gcgcaaatgc cgtgggtact
     6481 tcaggaaccg tactgacgca tctgatgaat cacggcgtag aagaaactat tatactgatg
     6541 aagcaatggc aagaagagct tcgtctgctt tatactatgg tcggcgcaac gaataccgcg
     6601 gccctacacc aacaatctct gatcttttcc ggaccagtga aggattggtg cgaagcaaga
     6661 gggatcgact tagtgaagta tggaagacgc aca
//
LOCUS       DACEOE010000141         6654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEOE010000141 DACEOE010000000
VERSION     DACEOE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.144"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            92..2737
                     /gene="polA"
                     /locus_tag="IW774_002195"
     CDS             92..2737
                     /gene="polA"
                     /locus_tag="IW774_002195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1154528.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            2782..3618
                     /gene="mutM"
                     /locus_tag="IW774_002196"
     CDS             2782..3618
                     /gene="mutM"
                     /locus_tag="IW774_002196"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1154529.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            3582..4211
                     /locus_tag="IW774_002197"
     CDS             3582..4211
                     /locus_tag="IW774_002197"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1154530.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            4263..4595
                     /locus_tag="IW774_002198"
     CDS             4263..4595
                     /locus_tag="IW774_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1154531.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            4907..5398
                     /gene="nrdR"
                     /locus_tag="IW774_002199"
     CDS             4907..5398
                     /gene="nrdR"
                     /locus_tag="IW774_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1154532.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            5421..>6654
                     /locus_tag="IW774_002200"
     CDS             5421..>6654
                     /locus_tag="IW774_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1154533.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQT"
BASE COUNT         2343 a         1180 c         1452 g         1679 t
ORIGIN      
        1 aattatttgg tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg
       61 aataagaact ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga
      121 tgggaacagt gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa
      181 aaatagaaac ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt
      241 tatgcaaaaa gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt
      301 tcggacagaa atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa
      361 agaacaaatg ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt
      421 gcccaattat gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca
      481 atttgatgtc gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt
      541 aaaagtggat atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt
      601 agcggaaaaa tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg
      661 agatgcctct gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct
      721 attgaaacaa tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag
      781 caaaatgaaa gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc
      841 gaccatcaat acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa
      901 aaatctcgat aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa
      961 attgaatatc gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga
     1021 attcaaagag gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa
     1081 ttatcataca gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac
     1141 gaacgatcta tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg
     1201 gctgaaaaaa gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa
     1261 aacgaaagga atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa
     1321 aagcacagat atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga
     1381 agctgtatac ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc
     1441 tcatttagcg cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact
     1501 ggcagataaa aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt
     1561 ggcagagatg gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg
     1621 cgagttctct gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga
     1681 attcaatttg aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc
     1741 cgttatcaaa aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg
     1801 tgaacaagca ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc
     1861 tacctatgtc gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta
     1921 tgtccagaca ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat
     1981 cccgattcga ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga
     2041 ctggctgatt tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc
     2101 aaatgatgaa catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc
     2161 gatgcgtgta tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc
     2221 aaaagcagtg aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct
     2281 aggaatcagc cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg
     2341 tgtcaaagaa tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac
     2401 gctgtatcac cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc
     2461 ttttgcggaa cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa
     2521 aatcgcgatg atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct
     2581 gttacaagtt catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga
     2641 taaattggtg aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga
     2701 cagcagttgg ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata
     2761 taatgaaggt gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga
     2821 ttggaacggt tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc
     2881 atcgagcaac cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc
     2941 attggacgta gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac
     3001 ttgcgaatgg aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg
     3061 catgtacttt tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt
     3121 ggtcgtatga caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg
     3181 ggacctgagc ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag
     3241 tcccacaaag caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat
     3301 atttatgtag atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca
     3361 ctgcgcccca aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca
     3421 atagaagccg gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga
     3481 tttcaagtag cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg
     3541 ccgatcgtga aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg
     3601 ctaaaaatcg caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa
     3661 gtacggcagc caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg
     3721 ccagagaaac tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc
     3781 aagaggtatt gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc
     3841 catcaaagga aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga
     3901 taaaagatca aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat
     3961 tgctctacga aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc
     4021 cagaaactca gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc
     4081 agagaattag tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg
     4141 acaacaatgg aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc
     4201 gctttctttg agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag
     4261 cgatgggatc attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg
     4321 gcgagatgct caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag
     4381 aagaaaaaga aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag
     4441 aagaagcgaa gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta
     4501 aaacaccgct cccagaaagt gaccacattt ccgatgagca aaaaatcgag gaacagcgaa
     4561 tgaaacacta tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact
     4621 tgaagcagat gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag
     4681 cttttgtgta agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag
     4741 taaggacagg ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt
     4801 tttgggtagg acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag
     4861 aaagacacat cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag
     4921 atgtcaacat aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat
     4981 ccgtcgccgt cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga
     5041 agctgctccg ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa
     5101 aattttaaga ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga
     5161 acaaatcgtc aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac
     5221 gaatctaatt ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat
     5281 tcgttttgca agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca
     5341 agatatcgtg gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga
     5401 aacaaaaagg aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt
     5461 accaaggaag aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt
     5521 atcagccact aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct
     5581 ctcaagaatc tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct
     5641 gcggtatccc gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg
     5701 tttattttaa agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac
     5761 tcattcctaa agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg
     5821 gtgaacgacg attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg
     5881 gatatcaaaa aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg
     5941 ctgctaatca agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag
     6001 cattacttga agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa
     6061 aacatattgc aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt
     6121 acggtttcga tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac
     6181 aaaaagtatc tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga
     6241 agcaaacaaa agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac
     6301 atttgacaaa cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat
     6361 atctggaagc aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac
     6421 tgcaagatct tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt
     6481 atgtactagt gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa
     6541 atgaatgggc acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa
     6601 taagtaatca agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actg
//
LOCUS       DACEOE010000142         6599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEOE010000142 DACEOE010000000
VERSION     DACEOE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.145"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            130..510
                     /locus_tag="IW774_002201"
     CDS             130..510
                     /locus_tag="IW774_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1154534.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            606..1115
                     /locus_tag="IW774_002202"
     CDS             606..1115
                     /locus_tag="IW774_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154535.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            1192..1371
                     /locus_tag="IW774_002203"
     CDS             1192..1371
                     /locus_tag="IW774_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154536.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            1426..1989
                     /locus_tag="IW774_002204"
     CDS             1426..1989
                     /locus_tag="IW774_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1154537.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(2036..2551)
                     /locus_tag="IW774_002205"
     CDS             complement(2036..2551)
                     /locus_tag="IW774_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154538.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(2577..3446)
                     /locus_tag="IW774_002206"
     CDS             complement(2577..3446)
                     /locus_tag="IW774_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1154539.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            3572..4252
                     /locus_tag="IW774_002207"
     CDS             3572..4252
                     /locus_tag="IW774_002207"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1154540.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4405..5385
                     /gene="pta"
                     /locus_tag="IW774_002208"
     CDS             4405..5385
                     /gene="pta"
                     /locus_tag="IW774_002208"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1154541.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            5508..5981
                     /gene="tsaE"
                     /locus_tag="IW774_002209"
     CDS             5508..5981
                     /gene="tsaE"
                     /locus_tag="IW774_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1154542.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            5974..6498
                     /locus_tag="IW774_002210"
     CDS             5974..6498
                     /locus_tag="IW774_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1154543.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
BASE COUNT         2183 a         1169 c         1391 g         1856 t
ORIGIN      
        1 tactttctgt tttttttgcc ttttttctta taaaaaggaa aaacagctat ttttttcaat
       61 ctgacacatt tacaatggaa ctggagatta atatagatcc gagcttaaac gaagaatagg
      121 agtgctcata tgaaccttac aacgatccat catatagcca tcattgtttc agattatcag
      181 aaatcacgtg atttttatgt taataaatta ggatttgaag taatcagaga aaattaccgc
      241 aaagaacgag atgactataa attagatctg aaactaggaa gtgctgaatt ggagattttt
      301 ggaattgcta atgcgccaca acgaccaaac tatccagaag cttgtggctt gcgtcatctt
      361 gctttcaaag tagacgatat tgaagaaaca attgcagaat tgcagtcctt agggatcgaa
      421 tcagaaccga ttagagaaga tacctttaca ggaaaaaaaa tgacgttttt ctttgatcca
      481 gacggattgc cattagaact gcacgaataa agataacaaa attgtcactt ttttttcgtt
      541 tcattcgatt tttcattcgt tcatttcttg atattcttta aaaaaaagaa acaggagaat
      601 gaagaatgaa aaaaatgatg tttggtgttg ctgccctcat cgtaataggg attaccggta
      661 tgacgggact gctcaatgcc gcttttcaca tggatgatat tagtgaccag gaatatcgga
      721 tctcattgaa aaaagcagca gcaatcgtaa aaaacgaaac aaaagaacat aacatatctg
      781 ctattcattt tgcagttgaa aaagatttgg agaatcctac gaatgcaaca tatgaatacc
      841 agtttttttg tgaaggacaa gtcatagcag tcaatccatc cactgggaaa acaaccgtac
      901 gaaatgacac ggaaaaggcc gcagaacatc tttttgatat agaaaaaacg gaagagttca
      961 aacaaccgca agcagcgatg aaggaagcag tcagctacta cgggaaaaaa gcaaaagtga
     1021 aagagtggac acttctagtc aaagatgaac gtccttatta taaagtccga ttaatagatg
     1081 gagaacagga ccgtcaggta tggatatctg catagaagaa ggatattgct tctctttttt
     1141 acaatataag agtaagctaa gagagtaaag atgaaaggaa gttgagataa catggaaaaa
     1201 atgaaatgcc ctaattgcgg aaaaaaattt gcatacgaag aagtcaataa tgtagtcgaa
     1261 cataatgata aagaaatgcc agtcgtctgc ccttattgcc gaactgaagc ggcaagaatc
     1321 gttactcatg gttatttcat tactgaaaag attgaagatt ttttaaaata acgattttgc
     1381 gatttctttt tctgcatgtt agaatggaga aaaggagagg aaactatgat tcgttttgcg
     1441 agaaaagaag atgctccaga aatcgcccca ttgatcttag tgattctaaa agatatggaa
     1501 ctgccatttt tgaagcagac aggggaacaa aagactttag agattttaga agaagcaatc
     1561 actgatccaa attatcgtta cagttattca cgaggtatcg tagaagaaat agacggtaaa
     1621 gttgcaggag ttgcttacgg ttatacagat gaagaagaat caatcatcga tcagccactg
     1681 gtacctatct tagagaaata tcagctagat gcgtctacac gtttgttcat tgataaagaa
     1741 acgtttccca acgaatggta tttggactct atcagtgtat cagaagattt tagaggacaa
     1801 ggcatcggtt cgcgattatt agaagcatta cctaaattgg cgaagaaagc taatcgctcc
     1861 gtcatcggtc taagtgtgga tgaaaaaaat ccaaaggcta aaaaattata cgaacgccat
     1921 gacttcaaag ttgtcggtca gcggatgatc agtggtcatc tttatgatca tatgcaaaag
     1981 aatatctaaa aagatgtacc tgaaatgcgt atttccgcat ttcaggtaca tctttttaac
     2041 ttaagttaag tcggtaagaa acaggcttat cgcgactttt gtctttacgg tagagccagt
     2101 aatctaaaac cgcagcaata caaatacaaa ctggagcatc ttcatcattt gagacatcca
     2161 atacatagta atctcctgaa aaaagtgttg ctttatccat tttcatgatt tgctgattga
     2221 aatgatggat ttgatattga tgattataga tatcaccttg gactgtccag tgaagtctac
     2281 gaatataata aaagtcaccg ggccaattga atattttctg catggttccc accttttcga
     2341 actctttata cagctcaaaa cgcgtaccga atgtcaggct tatctgcttg atgtgagcaa
     2401 cgagatcgcc attcatggca taaagagaca atgcatcgcc ctttgttccc cagcgaccaa
     2461 ccatcaaaaa cagggaatgt cctttttcgt ttttgacaat tgtccgagtt gcacgtccaa
     2521 gctgcttttc ctgaatgaaa aattctgaca tctatatccc tcttttctag ataaaactac
     2581 aagtcttctg caatcgcgcg caatatggct tttcctacgc cagattcaag gtttgtgtcc
     2641 gttgtatatt tggcataatc ttttacttcg atttctgcat ttcccattgc gaaactgact
     2701 cctgctactt gcagcatgct gacatcattg aagttatcgc caatcgtcat gacttcttct
     2761 aaagaaatcc cacgctcgtg agcgacatgg gcaacagcaa ttcctttttg agcgttttta
     2821 tggttcactt cgatattgtt ttgaccagag gaagtaacgg ctagatcgcc taaaagagaa
     2881 atctgctttc caactggacc taatacagct ggcccttctg tatgaaaaca aataattttt
     2941 aaaacttcca gattcttttc ttccaaaata gcacgaatac tgtccacgta tgtgatgtgc
     3001 aataactcca gcttagctga ggccatggca atcgccattt tatacgtaag gtgaggtaaa
     3061 tgggtagcca ccatggaagc aaaactttca attcgtttcg cttgattttc agaataaaga
     3121 ccatcgttgg ttgctacttc atagtaaata ccgttagcat ctagaatatc taaaacagag
     3181 attgcttgat tatttggaat cgacacggta aacaatgaat ttccttcttt atcaaatacc
     3241 tgtgccccgt tcaaagtgat catcgcacaa tcgattccta cctcatctaa agctgcgcga
     3301 gcttcttgat agtttcgacc agtagcgacc atgaactcaa caccggcatc atttgcaaaa
     3361 cgtatcgcct cggcattttc aggagaaaca ctcatatggg catctaataa tgtgccgtcc
     3421 atatctgatg caattaattt gatcataatg attatcgttc ctttctgctt cttgttccta
     3481 gtttaccata ttaatcaagt gctgagggtg aagattgtcc cttgtgattg aagaaaatgt
     3541 gagttattct ttttattagg aggtggagtt tttgaaagaa atcattcata acagttggca
     3601 agaagttttg tcctcagaat tttcaaaaga ttattatctg catctaagag aatttttaaa
     3661 aaaagaatat gcgtcccaaa agatccatcc agatatgtat catatctatg aagcattgga
     3721 attgactcct tatgaagaag ttaaagtggt gattttagga caagatcctt atcatggaga
     3781 aaatcaagct catggcttat ctttttctgt gcaacctggc gtaaagatcc cgccatcgtt
     3841 aagaaatatc tataaagagc tttatgatga tcttggaatc gcacctgttc aacatggaaa
     3901 tctagtgtca tgggctaaac agggagtcct tttactcaac accgttctta ccgttcggga
     3961 agggcaagcg tattctcatc gcgggaaagg ttgggaaaga ttaacagaca cgatcattga
     4021 aaaattaaat gaacgggaaa aaccaatcgt gtttatcttg tggggaaaac cggcacaaga
     4081 aaaaatcaaa atgatcgata agagtcgcca tatcatcata acatctccac atcccagtcc
     4141 gttatctgca agtcgtggct tttttggttc caaacccttc tcgaaaacga atgatgcgct
     4201 ccttgcgtta ggagaagaac cgattgattg gcaactgcct gaaacagtgt aataaaacag
     4261 cgctttcctg ttatagtgca gttcgctttg tatgtgaaaa acttaatttt tccagcattt
     4321 ttgactgata cagataaaat aaactgttct ttttttctgg aatagtgtat gataatacat
     4381 ataaaaacgt ctggaggtca tcttgtggaa ttgtttgata gtttaagatt taaaattgtt
     4441 cgccgtggga tcaaaatcgt tttcccggaa gctactgatg ctcgtatttt aggcgcagct
     4501 gcccggttga aagcagaaga acttgtggaa cccattttag tagggaacga agaagaaata
     4561 agaaatgcag cgcatgccag aggaatcaaa acatctagct ttacgatcat cagccctgat
     4621 aattacgata agtgggatga aatggtggat acttttgttg aacgtagaaa tggaaaggca
     4681 tccaaagaac aagcagaaaa gattttaaaa gatgtgaatt actttggtac gatgcttacg
     4741 tatatgggga ttgcagacgg aatggtctct ggagcgatcc attcgactgg cgacactgtt
     4801 cgcccagcac ttcagatcat caaaacaaag cctggcatca gtcggacaag cggagcgatg
     4861 atcatggtac gtggaagaga tcaagaaaaa tatgtctttt cggattgtgc gatcaacgtg
     4921 aatccaactg cgcaagaatt ggctgaaatc gccgtagatt ctgcaaaaac agcagaattg
     4981 ttcgatatcg aaccaaaagt agcaatgatg agcttctcga caaaaggctc agcgaaatcg
     5041 ccagaagtag acaaagtagt tgaagcaact aaaatcgcta aaaaactagc gccacaatac
     5101 atgatcgacg gcgaattgca atttgatgct tcttatgttc cttcagttgc acaactgaaa
     5161 gcgccagatt cagaagtagc tggtcaagca acagtctttg tatttccaga attgcagtca
     5221 ggaaatatcg gctataaaat tgctcagcgt ttcggtaact ttgaagcaat cggaccaatt
     5281 ctacaaggct tgaacaaacc ggtttctgat ttgtctcgtg gagcaaatga agaagatgta
     5341 tataaattat cgattatcac agcagcacaa acattgattg attaacaaat acacgcgaac
     5401 tagccaaaaa cagcagctgc tgttcttgac tagttcattt tttctttaaa aaaacttcat
     5461 tacgcgatat actggcaaaa agaaagaaga gcgggagaag atgaaaaatg atcgaactat
     5521 caggattaac aatgacagaa caattcgcag aggcaattgg tcattccgca atgccaggtg
     5581 acaatctgat attgactggt gatctaggtg cgggtaaaac aacgttgacg aaaggaattg
     5641 cccaaggatt agggataacg caaatgatca aaagtccgac atacacgatt attagagaat
     5701 atagtcaagg tcggattccg ttgtatcata tggatattta tcgggtagca gctagcggag
     5761 cagatttagg gttagatgaa tattttgaag gtgatgggct ttccgttgtt gaatggggaa
     5821 atctattaga agaagcactg ccggaggatt atttggaact gattttagaa aaaagtgata
     5881 cagatttaga aaagcggtat gtgaaactaa tctcatacgg ggagcaaagt gaaaccttca
     5941 aacagcgaat tctagaaaaa tgggctgaaa atcatgagtg aagaagttgg gatcatcttg
     6001 cgggaagcag tgccaggaga tgcaaaagac attcttttga tgatgggaca agttaataag
     6061 gagacagaat ttttagtttt ggatgaagca gaactcctgt taccaccaga aaccttagaa
     6121 gaagagttgg attatatata cgaaagcaat aataatttat tgctgcttgc aatctatgaa
     6181 gggacgatta ttggaacagc atctgtcaag gcagacagtc aatttcgttt gtctcacgtg
     6241 ggagaagtcg gtatcagtat tctgcaggaa tattggggaa tgggactagg cactcttatg
     6301 ctagaagaaa tcatcagttg ggcgaaggaa atgggtgtac tttttcgatt ggagcttgac
     6361 gttcaagttc gtaatgaaag agccgtacac ctttaccgaa aaatgggctt ccaaatcgaa
     6421 gcggtcatgc ctagaggggc aagaacagat ctaggtgaat ttttagatgt atataaaatg
     6481 agttatttga ttgaatagaa gaaataggaa aagagtgaga aactgtcatc cttttgagaa
     6541 ggcagaaaaa cacacgaagg gactgtgaca aaagtcgtgt cgcagtccat tttttttga
//
LOCUS       DACEOE010000143         6597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEOE010000143 DACEOE010000000
VERSION     DACEOE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.146"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..811
                     /locus_tag="IW774_002211"
     CDS             <1..811
                     /locus_tag="IW774_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1154544.1"
                     /translation="YIIIFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNK
                     KLADQLLEQQVRMQQDYIKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYY
                     DQTILPTRQMMNLFPANLSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELD
                     TKHTVNLVRMLGIILDNAIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQL
                     KQKGFSTKTNPKNEGLGLFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            824..1552
                     /locus_tag="IW774_002212"
     CDS             824..1552
                     /locus_tag="IW774_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1154545.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(1621..3144)
                     /locus_tag="IW774_002213"
     CDS             complement(1621..3144)
                     /locus_tag="IW774_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1154546.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            3396..4577
                     /locus_tag="IW774_002214"
     CDS             3396..4577
                     /locus_tag="IW774_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1154547.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(4637..6265)
                     /gene="treC"
                     /locus_tag="IW774_002215"
     CDS             complement(4637..6265)
                     /gene="treC"
                     /locus_tag="IW774_002215"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1154548.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
BASE COUNT         1873 a         1284 c         1138 g         2302 t
ORIGIN      
        1 ctatatcatt attttttata cccgtttttc tggtgaaaca cctgatgtct tagctttgaa
       61 tacgagtttt ttcattctat atctgtgtgt tggcctgatc atcttggttt attattggaa
      121 aatctctaat aagaaattag ctgatcagtt attagagcaa caagtcagga tgcaacagga
      181 ttatatcaaa gatctagaaa aaaactatca agacttgaga gaattcaaac atgattacca
      241 aaatctgctt ttttctcttc acagctatat ttcagaaggc gacttagaag gattacggtc
      301 ttattatgat caaacgatat tacctacacg ccagatgatg aacctttttc cggccaatct
      361 aagtatgttg gataatatga aagttcctga gatccgtagt ttactgtcat taaaactcat
      421 gatggcacag gaaaaagggt tgatcgtcca gcttcatttt cctgagccaa tcgaactgga
      481 tacgaaacat acggtaaacc ttgtacgtat gcttggtatt atcttggaca atgcgattga
      541 aggagctagt gctgcaaaga agaaaaaaat cgaattgacc atactcaaga agaatcattc
      601 cgtgatgatt cgtgtgatca atacaacaac aaatacatta ccgttaaacc agttgaaaca
      661 aaaagggttc tcaacaaaaa caaatccaaa aaacgaaggc cttggcttgt ttatcttgga
      721 tgaattgaca aagaccaatc cttatctttt ccttgaaaca tcaattgaaa accagcgttt
      781 ttctcaaacg atttatatac aaataaaata aaggagtaat gatatgattc ccatttatat
      841 ttgtgaagac gatccaaaac aattagaagt tttatcaacg gtgatcaaaa atagaatcat
      901 gattgaaaac ttagacagtt atgtccatat ttctacctct gatccggaag aacttctcaa
      961 tgcagccaag atcagaactt cttcttttgg tatttacttt ttagatatcg aactagaaga
     1021 cagtgaaatg gatggtgtag cgatcggtaa attgatccgt gaattagatc ctttaggaaa
     1081 aataatctat gtgacttccc atacggaaat gtcgatgaaa atcttaaaaa gcaacatcga
     1141 accgactgat tatatcgtca aagaagatct ttatgattta aaagaaaatg tcgagcatat
     1201 cctgagcaaa attttcgaag attttcaaat gtcccaaatc caaaaagata tcttcaaaat
     1261 agagtttaat gatgagatca aatttttacc gatcaaagac atccaatatt tttctacttc
     1321 gctaggagcg ccacataaac tggaagtcca tttgactcat gcccaattac aattctatga
     1381 gaagatcaaa gaaatcgaga aaatgcatcg gaattttatc cgttgtcata aatcctatgt
     1441 tatcaataca caaaatgtgc ggtcgattaa taaaaaaacc agagaagtca cgttggcaaa
     1501 cggcgagacc atcccagcct ctatccgtgg attaaagaaa ttgatttcat aaaaagaacc
     1561 gaatagtttg gaccgactaa atgaccagcc gatccaaacc cttcggttct tttttgccta
     1621 tcagtgcaaa agcatttcct gtgcaatttc acgtccatcc attgtatctg gataaaatgt
     1681 tggccaattc tccatttctt tcagcaatac ttcttgactt tcatttcctg catataaatg
     1741 aaaatgttcg gagtcagccg gtgcaatcgt gtgatcactg aattgaatat atctaaaagc
     1801 gccactttcc ttatcttctg cttcgaatag ataccttacg ccacggtttc ctgcctcgta
     1861 ggtcaagatt tctttcccta cataacgata atttgaacgt ttcgttgttc cattcttaaa
     1921 cgtaaatgcc atctctttct cactgatctt gattgaagca atatctgttt gatagccctt
     1981 cgtataatat tctttatatt cttgtgctgt cttatcctga ttcaattttt ctttatattc
     2041 aaaaacagga tcaaaagtcc catcttctac atacgtatct actgtttgcc aagttcctgc
     2101 ccaatctgaa agagagcgat cttgtacatc ctcgtcttca aaataaccgt tgtagacgct
     2161 cttttctttt ttgggggtca aagtatcttc tagcgggttt tctgtctctg tcgttttttt
     2221 caacgcttcg aggtttgctt ccatgataga aagatagttt tctccatttt ctatttgttc
     2281 attcgtcaat ccttccaaag gattcaatac ttctaaagat acacctgctt cgtttgctaa
     2341 cgttctggca atactatctt tggcgttttc ttcaaaataa atatagttga ttccatgttc
     2401 tttaacaaag tgtttcagtt cagcaattct cgccgcagac ggttcttcac tagaagaaag
     2461 accagcaatc gatacttgat tcaatccata ttctaacgct agatagttga atgcagcatg
     2521 ttgcgtgaca aaagatcttt gttttgcttg cttaaaggtt tcttgataaa gctgatggag
     2581 tgcttgaagt ttctcggtat aggctgctgc attttttgtc catacttcct gtttttctgg
     2641 gtaagcttca atcagctgat cacgtatgtt tgctacctgc ttgattgcta aacttggagc
     2701 tagccataca tgtgggtcta gctcatgtgt atggccttct tccccgtgtt catggtcatg
     2761 atcatgtcct tcttcacttc ccggaagtaa aagcatacct tctgtcgctt ttatcgtatg
     2821 gacattgcct tcttgtaatg ttttttggat tgcggggacc catgtttcca tgttttcatc
     2881 attataaaca aagatatctg tatcttggat ctttgccatg tctttagcag aaggttcata
     2941 atcgtgggaa tcagtccctg ctggaatcaa taactctact tctccttcat ccccgacgat
     3001 tgcttttgta aaatcataca tgggataaaa ggtcgtcatc acgctcaatt tttctttact
     3061 gtcctcggct ttcccacttg ccccgcaagc agttaacata cctgctaaaa ccagcatggc
     3121 caatatacct atcaatcgtt tcatttatga cactcctctt ctcatcttct ttcatttgaa
     3181 tgttccgatt cgcttttcgc aaaccgtaat agttacgttt tagcaacagg atttaattta
     3241 tcatatggtt aaaagaactg tcaagcgttt tcttatttta attgatgaga aagtttatat
     3301 gaatgattga cgatattttt gcaatcaacc tctatcattt tccttttata cccgcttttg
     3361 tctataatga agttatagat aaaggaggaa aaagagtgaa aaaagaaaca gatacaacga
     3421 atcgtttgat ccgcttttta ggaggaaaaa cctcttacta tgttttagga atagttatct
     3481 tacttgcgct gatcatttat tttttccatc agatctcgtt tatattcaag cctgtcggaa
     3541 tcattatcgc tactatcttg cccccattat tgtttgcact gatcttgtat tatcttttgg
     3601 aaccagtcat caaacgtttg agcaagcacc tttcgaggaa cttgtcagtc attatcgttt
     3661 atgtgattat tttattttta ttggggcttg gaagcatatg gcttattcct tttcttgaag
     3721 aacaggcaaa gacattgatc agtagtttgc cagatttgtt tcaagatttt caagagagtc
     3781 tccgacaatt tttggagaaa acaccttttg ccgattcatt taatcaattt ttcgcttcta
     3841 ttgacgacgt gacgtctgat atagctggat ttattggaaa ttattgggaa agtggcgcac
     3901 aacggatcag caacattttt tcaactgtta cagctatttt tattacttta tttactggtc
     3961 caattgtcgc ttttttcctt ttgagagatc ctcaaaaatt ttatcagtca gttttagcga
     4021 ttgtcccacc agcatttcgt acagatttta aaaatttgac aaagattgct aatcaacaat
     4081 tgggttcatt tctaaagggg caaatcattg cttcatttat ccttggagca gtctactggg
     4141 tttgtttctt actgatcgga ttagaatttg ctagtgtttt agctatttct gctgggttgt
     4201 tatgtatcat tccatatatt gggccgttta tcgccttctt tccaggtttg atcattgcat
     4261 tccaagactc tacttttatg gctgtcaaat ttgtcattgt ctggtttgcc gtacaacttc
     4321 tccacggtga cttggtgatt cctcgagtaa tgggtgatcg tttacagatc catccgatta
     4381 cgatcttaat cgttttactt gtcatgggtg atctaatggg aattgtgggc gttatttttg
     4441 gtattcctat ctatacgtta gtcaaattac tcgttacttt tattttccgt aaattcaaac
     4501 aacgctacaa taaattttat ggcgataaag gagagtatga aaactcttca ttttctgaag
     4561 aagattattt taaataaaac acaaaacgtc gatcttacga gaatagctcg taggaacgac
     4621 gttttgtgtt tttgttttaa tttttcagca aagcaattgc ctggtatggt tttagctcca
     4681 gactttgatg aagaacagta tcggaataat tatcgataag aatattggct tctgtgaatc
     4741 cttctgggat ggcgatcctt gtatctttat catagaaatt tgttaataca agcagctgct
     4801 gtccttcaaa ttggcgaaga aagccataaa cttccggatg atttgcttca taagcatgat
     4861 aatctccttg tgcaacgatt ggatatgttt ttctcagccg aatcaatttc ttgtaatagg
     4921 acaaaataga atcttcagaa gatagttctt tttctacatt aatctcttct agcttgcctc
     4981 caacttttaa ccaaggtttc cctgtagtaa atcctgcttg ttctgtgtct gaccattgca
     5041 tgggtgttcg agagttatcg cgagattttg ctttgatccg acgaaacgct tctttttctg
     5101 tgaatccttg ttcaaggagt aacttataag cattcaaact ttctacgtca atatagtcgg
     5161 aaatcgatga atagtctggg tcgatcatgc cgatttcttc tcccatataa atatagggcg
     5221 tcccacggtt caaatggatc atggttgcta gcatctctgc tccttgtttt cgatactgat
     5281 cgatagctac aaatcgattg agcgcacgtg gctgatcatg attattaaaa aatagagcat
     5341 tccagccgtt accctcactc atcttttctc cccatgtatg aaatagtcgc tttaattctg
     5401 caaaatcaaa tggaggagta gaccatttat tcccattttc ataatcgact ttcaaatgat
     5461 ggaaattaaa gaccattgat aattcttctc tactaggttc tgtatacaag atacaatttt
     5521 cgatcgtcgt agagctcatt tcaccgactg tgatgatagt ggaatccgtc ccaaacgttg
     5581 cttggttcaa ttcttttaga tacgtatggg tgattggttt gtccgtatat tcaggtttgc
     5641 catcattttc tttattattt ttcaattgtt cgtctttacc gataacattg ataacatcga
     5701 aacggaatcc ttttacccct ttttccatcc aaaaacgaac aactttgaat agttcctctc
     5761 ttactgcttg gttccgccag ttcaagtcag cttgtgtccg atcatataag tgcaaataat
     5821 atttccccgt atctccaaat ggcgaccaag cacttccacc aaattttgat acccaatccg
     5881 ttggctcttc tcttaaaatg aagaagtcct gataatgctg gtcccctgct agtgcttttt
     5941 tgaaccattc atgttctgtc gaaacgtgat tcagcaccat atcgagcatg atttctatcc
     6001 ggtatttctt tgcttcggct attagctgtt cgaattcctc catcgtacca aagataggat
     6061 cgatggctgt gtaatcagag atatcataac cgttgtcttt ttgagggctt ggataaatag
     6121 ggtttagcca aatcatgtcg atgcctagat cattcagata cgggagtttt tctttgattc
     6181 cttttaaatc tccaattcca tcaccattac tgtctttgta agatttggga tagatttggt
     6241 aaatgacttt gtctttaaat gtcatggata tttacctcca ttcaactgat ttattaccta
     6301 ccacaactta tatatacaag ttatcattta ttattataac tgaaccgaaa gcgtttgtaa
     6361 aatttatctt tatacattct aagaggtctg ttgcccaata aaaaacaaga gatcagacat
     6421 gtatttgtct atcctcttgt ttaaaatagc aaaatattct tgtttcttct ataaatggtg
     6481 ttcaaaagct tttatctacg gcaatcagtc ttctcaaatg taaaacatga ggagctaatt
     6541 ttatttgagt attctttttg ctcgatgcag gacggctttt ttacaacctc tgattca
//
LOCUS       DACEOE010000144         6527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEOE010000144 DACEOE010000000
VERSION     DACEOE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.147"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            52..789
                     /locus_tag="IW774_002216"
     CDS             52..789
                     /locus_tag="IW774_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1154549.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            817..1356
                     /locus_tag="IW774_002217"
     CDS             817..1356
                     /locus_tag="IW774_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1154550.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(1854..2315)
                     /locus_tag="IW774_002218"
     CDS             complement(1854..2315)
                     /locus_tag="IW774_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1154551.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            2448..2822
                     /locus_tag="IW774_002219"
     CDS             2448..2822
                     /locus_tag="IW774_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ1154552.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            2876..5002
                     /locus_tag="IW774_002220"
     CDS             2876..5002
                     /locus_tag="IW774_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154553.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIF
                     VSFTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFM
                     IFLINLITSIIIYKKNSFKYFKENIL"
     gene            4999..5619
                     /locus_tag="IW774_002221"
     CDS             4999..5619
                     /locus_tag="IW774_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154554.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            6312..>6527
                     /locus_tag="IW774_002222"
     CDS             6312..>6527
                     /locus_tag="IW774_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1154555.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQ"
BASE COUNT         2564 a          911 c          906 g         2146 t
ORIGIN      
        1 ttttacatcc tcttatgtct atgataacat aatttattga ggtgattttt attgagtaac
       61 gatatagttc gatataataa tggattaaat tctgtccctt taagaaaatt tacccctata
      121 gatatggatt tattttgggg aatatgttct aaaatgaaaa gaaaaggtac aaaagaagaa
      181 atttttacat ttaaacaaat aaaagaatta attgattaca atataaatgc acgtggcgac
      241 cgttttgcta atgatttaat aaaaatgtca aaaaaaatca ttactttgtc ttttatttat
      301 gaagacgaaa aaacatatga acaattaaat ctgtttcaaa aatttaaaat cgataaagtg
      361 aatgaaacat tattaataaa ggtttctgaa gaatttgaat atatacttaa ttcaatcgga
      421 actaatttta ctcgtttcga attagaaaat atgacaaact taaagagtag ctacgttaaa
      481 gagttatatc gccaacttat ggctcataaa gacagaagat ctaaaaaagg tcactggttt
      541 gtaaaaatag aagattttag agctctatta gcaataccag aaagttatcg catgagagat
      601 attgataaac aaatttttgc ccaagcgaaa aaagaatttt taaacgttaa ttcaactcac
      661 cctgctattt ttaaaacttt tgaaatcaaa aaaattaaag cctacaaagg aaataagata
      721 ggatctctgc aaatttattt tgaagaaaat ataccttcaa ttaccttaga taattggtta
      781 gagaaataat acctaacaat ttaggaggca attatcatgc aacccaaaga aacctttaca
      841 ataaaggaat tatctgaatt atttgaaata agtaggcaag caatctctaa acatgttgat
      901 aaattagacc cgtccttatt agctaaaaat gagcgtggtt ataaggttgt attacgttct
      961 ggagtattgc aacttgctag caacttaggt aaccaaaggt tactatatag gttgcatgag
     1021 tatgaacaag aaaacgataa aaacgtttat atatcaagca aaaacgtgat tgtaaacgag
     1081 ttgctagata agttgcgtga agaaaatgaa tttttacgag aacaactttc cctcaaagag
     1141 caaaataatt ctaatatgca aaaattactc gaccagcaac aacagctcac cctacaggca
     1201 aatcaacaga tcgaaaaact gcaagaacag ttacaattag cttatacgga agaaactcca
     1261 gaagatgaat caactactat tctttctgaa gaaacagaat cgatagaaaa acacacaaaa
     1321 aagaagaagc aaaaatggtg gcaactttgg aaataactgg agggaacatt catgacaaga
     1381 aaaaatcttc tattcggttc tattttacta ggaattctag gcttcagtcc gatgttattg
     1441 tcagtaatca ataaagggaa taaacatggt gaattgaaat tgaatccaaa ttattacaac
     1501 agtcctaacg aagaattgtt tgtaaaagag acggataaat aataaaaaag actgctcttc
     1561 cgagcagcca gttaaaaaat aatgagtgtt tattcttgaa aaagcacgcg cttatgaagc
     1621 gctaccaaaa atttaccata ttttacaaaa aataacaagt cttaaaaaaa tgacagataa
     1681 aaatgcacaa agaattagat atagataacc gcgaaaaatc aagctttaga aagttgcttt
     1741 tagctagacc aattaaaaat cacaaagtga gcgtctctat ccattggtag aggggctact
     1801 tggcatagtg tacattatgt caactatgca attaaataac taactacttc atattagctt
     1861 aataaaataa tcaaaataac tagggggaaa aacattgtat aaaaaaattt ttcatttttt
     1921 aaataagaat agtaaatata aaaataattt aagatatcta ttactattaa tccagataga
     1981 gcaaaccaag taaatattcc atgtgtaaag ctaaaaaaat tcatttgaat caattcaaat
     2041 aaacctaata tagtaacaaa tatattaaaa ataatagctg tcaataccag taaattattt
     2101 tttttatttt ttgaattatc ttcatttaaa atagaattta tgttagtact ataaaattca
     2161 caaatttttt ttagtgtctc tatatctggt gttgttttgt tattttccca ctttgaaata
     2221 gtttgacgtg taacgttaaa ttttcttgcc acatattctt gagtataccc ttttttcact
     2281 cttaattctc ttagcttaga tcctaaatcc tccatttttt ctcctatgta aacgattatt
     2341 tacataaaat tttatcataa aaatactaac gcgccaaaaa agttctttat tttataaaac
     2401 acactaaaat aaaataattc tagtaaaaaa gataggagaa ataagttatg aaaaaatttt
     2461 tatgtctttc tgctttaatg ggtgtcttat taactagtgg aggaatagct agtgccacag
     2521 aagctttaaa tctagatgta cccgaagacc ataccgccat ttacggagga ggaatggaag
     2581 ctacagaaga aggattttct tctagtaaat tgagatatgc tgctggcggc ggggatttca
     2641 actgtggggt taatggattt aaagtttatg caaattacta ccatgctaga gctaaacata
     2701 gtgcaacagc taaaaatgga cgaggcggtc aagtacgatc agttcagaaa gctggagtaa
     2761 gagcttatgc aacatgcaat gccacactta ctggaaatac tggttggtgg aatgtttatt
     2821 aaaaaagaga gtttaaactc tcttttttat ttacatttga aaggaaattc aaaatatgtt
     2881 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
     2941 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
     3001 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
     3061 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
     3121 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
     3181 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
     3241 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
     3301 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
     3361 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
     3421 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
     3481 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
     3541 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
     3601 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
     3661 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
     3721 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
     3781 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
     3841 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     3901 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     3961 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     4021 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     4081 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     4141 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     4201 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     4261 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     4321 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     4381 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     4441 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     4501 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     4561 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     4621 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     4681 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     4741 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     4801 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     4861 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     4921 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     4981 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     5041 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     5101 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     5161 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     5221 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     5281 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     5341 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     5401 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     5461 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     5521 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     5581 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     5641 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     5701 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     5761 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     5821 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     5881 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     5941 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     6001 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     6061 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     6121 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcactaa
     6181 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     6241 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     6301 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     6361 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     6421 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     6481 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccag
//
LOCUS       DACEOE010000145         6297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEOE010000145 DACEOE010000000
VERSION     DACEOE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.148"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            396..1229
                     /gene="msrA"
                     /locus_tag="IW774_002223"
     CDS             396..1229
                     /gene="msrA"
                     /locus_tag="IW774_002223"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1154556.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1309..2298
                     /locus_tag="IW774_002224"
     CDS             1309..2298
                     /locus_tag="IW774_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ1154557.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2587..3285
                     /locus_tag="IW774_002225"
     CDS             2587..3285
                     /locus_tag="IW774_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ1154558.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3435..4751
                     /locus_tag="IW774_002226"
     CDS             3435..4751
                     /locus_tag="IW774_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1154559.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            4768..6183
                     /locus_tag="IW774_002227"
     CDS             4768..6183
                     /locus_tag="IW774_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1154560.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
BASE COUNT         2118 a          998 c         1332 g         1849 t
ORIGIN      
        1 cacttcctgt atatgcttat ttaggaacga atgtagaaat aaatcatcaa cctgtaaagg
       61 agcaggtgaa tgctaatgga ttggtacaaa taagcggagt caaaggagtt gctgatatta
      121 aagtaagcac aagttataca gtaacgacta ttatttctta tcttgttaca ttaactgcat
      181 atttatgttt attttttaaa atagttttcc ataaaaggta aattaacttt tcatttataa
      241 aataaattga ttaaaaagga gaatctgtcg ataaggattc tctctttttt atacgaaaaa
      301 tctgtagggg gatgaagtaa tcgcttattt cctcacttta tgataaatta cagctaaagt
      361 cactagcttg tcttcttcgg aaaggaggag aatctatgga tgaggaacaa atattcattc
      421 aaacacttta taatctgatt ttgaatccga atacacgaga ttgggaacga aaagtattga
      481 ttcagacaaa gaatgacata agagaaaata tatccgttaa agaacagtta tcaaaactag
      541 aagcaacact acgtccatta gctatccgaa tgaatctgac gcctgatgta atggactttt
      601 atttattgat tacggaagga tttgataagg agcaaaaata tgacttttcc aagcatgcca
      661 tgcaagatgc tgactatcaa gaacgtgcag tatttgcagg gggatgtttc tggtgtatgg
      721 tcgagccgtt tgaatcgaaa aaagggatcc tatctgtttt gtcaggatac acgggcggac
      781 atgttgaaaa accaaattat gatcaagtaa gtggtggtta cacaggtcat gtggaagctg
      841 tggagatcat ttatgacaca agagaaataa gttatagcga gttgctgaca atctattggc
      901 aaattactga tcccacagat acatttggac aatttcaaga tagagggaag caatatcgtc
      961 cagtgatttt ttatcaaggc gaacgacaaa aagaattagc ggaacaatca aaacagaaac
     1021 tagacagttc aggaactttc caccaaccaa tcgtcacaaa aatcgaacca gccggtacgt
     1081 tttggccagc agaaaactat catcagcaat tttacaaaaa acaaccgaaa cgttataaaa
     1141 aaatccagca agcacgtaat caatttctga tctatcaacg agtaaaaaac aaatggcaaa
     1201 aaaatatacg aaaaaatcat tttgattaac tccttttctt taaaatgtag taaactaaaa
     1261 atagaaaaaa gaaagcgttt taaaaagatt tgatgaggag gagaaaaatt gtatattgca
     1321 gatagtgcac gttatgaaaa aatggaatat cgtaaatccg gtcattcagg tttgaaatta
     1381 ccgattttat ctctcggatt atggcaaaat tttggtgatt atgatcctat ccataatcaa
     1441 agagagattt taagaggcgc attcgacatg ggaattaccc atttcgacct tgcgaataat
     1501 tatggaggcc ctgctggagc tgcagagaaa aactttgggc gtatttttag agaagatttt
     1561 caagcctacc gtgatgaact gattatttct agcaaagcag gttatcatat gtgggaaggg
     1621 ccatatgggg aatggggctc tcgtaaatcc atcatttcaa gttgtgacca aagtctccaa
     1681 agaatggggc tagattatgt tgacatcttc tatcatcatc gcccagaccc tgatacacct
     1741 ttggaagaaa cagcagaagc tttgatgcaa cttgtaagac aaggaaaagc attatatatt
     1801 ggtatatcca actataatgg agaagataca aaaaaaatga ctgaaatttt aaaaagaaag
     1861 gaagcaccgt tcattattca tcagatgcgc tacaatatgt tttctcgtgc actactagaa
     1921 gacgatctat ctccagtatt agaagaagaa gggctagggg caatcacgtt cagtccgctt
     1981 gctcaagggt tgctgacaaa tcgttatcta catggtattc ctgaagattc tagagcacat
     2041 cgtaaggaaa taccattcct ttcagaagaa caagttggat caactctgga aaaaatcaaa
     2101 gcactgcaaa caatcgccgt atcacgagga caatcattag cgcaaatggc ccttgcatgg
     2161 aatttgcgac aaaaatctgt tacaagtgta ctggtcggag caagtcggtt aagtcagtta
     2221 caagaaagtg ttcgaatgat ggataattta gatttctcac cagaagaaga attgagaatc
     2281 gaccaaattc tagaatagaa acgatgtaat caaacttttt atccatatat taaaaaaaac
     2341 aaaagatgac atcaagaatc gaatgaagat cgtctgttat tgaacatcat attttttgtc
     2401 gttcaaaata aaaatgatcg aaggctagga gtgaatttta tcctagtctt ttttatggta
     2461 aatatcttct tcgtttgtat gtctatgaac ggattaatca attttgtata caaaaaatca
     2521 tcagatgatt caaacgtgtt ataataatta cgaaaaaata catcagatga aaaggaggta
     2581 tgtggtttgg aagaaaataa aaccctagta gaagtaacta ctgacaagat ccttagatat
     2641 ttaaaggaaa tgcagttgca agtaggagat aaactgccga atgaatatga attgtccgat
     2701 tatttagagg tggggcgaag cacggtgcga gaagctgtgc gggctctagc ttccaggaat
     2761 atccttgaag ttcggcaagg agcggggaca tttgtcagtg ataagcgagg gattgccaat
     2821 gacccactcg gtttttcttt gattgaagat caggatcgga tgatacgaga cttatttgaa
     2881 ctgagatatt tattggagcc taagatggca ggtttggcag cggaatatgc aacgaagaat
     2941 caaatcgtcc aaatgaaaaa attaacgcaa aagattgaaa cttcttttcg agaaggcaca
     3001 caagaacatg ttcaattaga catcgatttt catacgatga ttgcagaagc tagcggaaac
     3061 gtagccttgc tgcatattat cccaatcatt aatgaatcaa ttgctttgtt taataaaaat
     3121 tatgatatcc agcatctgaa aatcgagacg atcgatattc atacagagat cgttcaggcc
     3181 atcgaaaggc ggaatgcact tgctgcgttt gatgcaatga ctgtccatat ggcaataaat
     3241 cggagagaat tacagaaaat aataggagaa aaagaaacta attgacagtt catctgatgt
     3301 attttatgat aaatacatca gatgaatttt tgttttatat ttggtaaaaa agaggagtaa
     3361 atagtttctg ttcatcgaaa tgtaagcgct gactaataga caaaaagaga tgacgtcaag
     3421 gagaagaagc aggaatgagg ggatctggaa tgagttttat ggataaatta gccaatgttt
     3481 tagggaacgt ggcaacgaga atcaataatt taaggtatat catggtgata aaaaacgcat
     3541 ttgctgcatt gattccggta attattacgg gtgcttttgg cacattgttt tcagcaatgg
     3601 tctttgactc ggaaaatggc ttagcacaga taagtttctt gcgtttttta gaagaattaa
     3661 agcctatcgc atctgccatc agctatgtca cactaagttt cttgaccatc tatgctgttt
     3721 ttctgatagg gatcgaatta gccaagctga ataaagtgga tggtgtattc cctggtatca
     3781 tcgcagtgat gtcttattta tctgttaatc caactgttta tgaattagtg aatgaaaatc
     3841 agactgttat agtagaaaac gtattggcta aacaatatac agatacgaaa gggttatttt
     3901 tgggaatgtt ggtctccatc ctttctattg aactttattg ctggttgagg aaacagaaga
     3961 aactacagct gagaatgcct gataccgtgc caacgaatgt ttctgctagt ttttctgctt
     4021 tattcccgac gattctgact gtatcgggta tcgcggctgc gggatttatc atcaaagaac
     4081 ttacaggaat gtacctttat gatatcattt acaatgtcgt acaacgccca ttagaaggtg
     4141 tcgtacaagg gttaccgggt attttgctat tgatgtttat tgcgcaagtc ttttgggtga
     4201 ttggtatcca tggaaatcaa atggtgaagc cgatacggga accgttgctt ttagcggcaa
     4261 ttgctgtaaa tacggaagct tttgaagctg gaaaagaagt accgaacatt attactatgc
     4321 cattttggga tatgtatatg agtatgggcg ggtctggagt cacgatcggt ttactgattg
     4381 cagtattgtt agttggaaaa cgggaagata tgaaacaaat caccaagtta tcccttgctc
     4441 ctgggatctt caacatcaat gaaccagtta tttttggtat gccgatcatg ctgaatccaa
     4501 ttttagctat tccgtttatc ttaacgcctc ttatcactgg aacgattggt tattttgcaa
     4561 ccgctttagg atttgcggcg aaagcagttg taatggtccc ttggccaatg ccgccgatcg
     4621 tcaatgctta tctggcaaca gcaggaaata tcggtgcagt tattacgcaa cttgtttgta
     4681 ttattgtttc tattttgatc tatctgccat ttgtaaaagt agcaaatcgt gtggaaaatg
     4741 atgggatcta aaaaacagga ggaaatcatg aaaattacta ttccaaaaaa ttttattctt
     4801 ggtgctgcat cttctgcttg gcaaacggaa ggttggaaag gaaaaaaaga aggacaggat
     4861 tcgtttatag atagctggta taaaaatgaa cattttgttt ggcataatgg gtatggccca
     4921 gccgtagcaa ctaattttat ggaacaatac cagcaggatg tggatctgat gaaatccatc
     4981 catctgacac attatcggac ttctatcaat tgggctcgtt tttttactga ttatgagaac
     5041 tgtatagtag atgaagaata tgctcagcat ataagtgaaa tgatcgactc tttgatcgaa
     5101 gcagacgtag aaccgatgat ttgtttggaa cattatgaag tcccagcttt tcttttggaa
     5161 aaatatgatg gctggtcttc aagaaaagta gttgattttt atgctaagta tgctcaaatt
     5221 gcctttgatc gttacggtga tagggtaaaa aactggttta cattcaacga accaatcgtt
     5281 ccccaaacgc gttgctattt ggatgctgtt cgctggccgc atgaacaaaa cacaaaaaag
     5341 tggatgatgt ggaattatca taaagtttta gcaacggcta aagcagtaga gatttaccgc
     5401 agcggaaatt atggtggaag gattggctgt attttaaatc ctgaaatggt ctatgctcga
     5461 tccaattctc cagaagacca aaaagctgct tatatgtatg atttgtttta taatcgagta
     5521 ttttttgatc cgatggtaaa aggatactac acagaagagt tcctggagtt gtgtcaaaaa
     5581 caatcgattt atttcgatcc tgcaacagag gatttagaaa cgattcagca tcatacagta
     5641 gatttccttg gaatcaatca atattatcct aaacgcgttc gcgcccctcg ttatcaatgg
     5701 aatgaagaga cacctttcca tcctgaacga tattatgaag tctttgattt acctgggaag
     5761 aaaatgaata attccagggg atgggaaatc tatccgaaaa tcatgtatga tatagccatg
     5821 tatctaaaag aaaattatca taatatccct tggctgataa cagaaaacgg tatgggaaga
     5881 gaatatgaag aacagtatat ggatgagaaa ggaatcgtgc aagatgatta ccgtatcgaa
     5941 tttatcggac agcatttgca ttacttgatc aaagcaacag aagaaggcgc aaattgcgaa
     6001 ggctatatgt tatgggcatt cactgactgt gtgtcaccaa tgaatgcatt taagaatcga
     6061 tatggattag ttcgaatcga attgaacgat gaacgaagca gaacattgaa aaaatctgct
     6121 tcgtggtatc agactttgat tgataagaga gaattggatt tgcctgacga aatctataaa
     6181 taaatagaag ttttttgtag atgttcattg tatttactgg ttatttgaaa taaaagaggt
     6241 tgtgaatcca gcgttttgag ctaataccca gggtcagctg attgaacacc gtttatt
//
LOCUS       DACEOE010000146         6156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEOE010000146 DACEOE010000000
VERSION     DACEOE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.149"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..586)
                     /locus_tag="IW774_002228"
     CDS             complement(5..586)
                     /locus_tag="IW774_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1154561.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLVKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IW774_002229"
     CDS             complement(769..1395)
                     /locus_tag="IW774_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1154562.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IW774_002230"
     CDS             complement(1417..2748)
                     /locus_tag="IW774_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1154563.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IW774_002231"
     CDS             complement(2903..3304)
                     /locus_tag="IW774_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1154564.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IW774_002232"
     CDS             complement(3390..3596)
                     /locus_tag="IW774_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154565.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IW774_002233"
     CDS             complement(3691..5193)
                     /locus_tag="IW774_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1154566.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW774_002234"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW774_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1154567.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5712..6156)
                     /locus_tag="IW774_002235"
                     /pseudo
     CDS             complement(5712..6156)
                     /locus_tag="IW774_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         1622 a         1347 c         1011 g         2176 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gaccaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt tttacataca gcataggatt ttgcttgatt
     5761 aatccccaag gataaaccgc catattcagc gcattcttaa ggacagagtg agtaatagtc
     5821 attgttttct tcgagtaacc ctttttaaat tcagcattga tgaaattttg taaaagagca
     5881 ggggagagat ccgtaagttt tttctttcct aaataaccgt ttatatgatt tttgatggta
     5941 aatcggtagt tttcataggt attgtatttt agatttagtt taacgtattc ctccatccaa
     6001 aaatcaaggt attgttttac tcgagtatcc gtacctaaaa agtattgtcc tgtttcgtca
     6061 atatctgata aaacttttcg taaagcagct tcggcctctg gtcgggtgtc tccgccaact
     6121 ttctccactt tttttcttga gccatcatca ttgata
//
LOCUS       DACEOE010000147         6073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEOE010000147 DACEOE010000000
VERSION     DACEOE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.150"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            234..842
                     /locus_tag="IW774_002236"
     CDS             234..842
                     /locus_tag="IW774_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1154568.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            1010..2647
                     /locus_tag="IW774_002237"
     CDS             1010..2647
                     /locus_tag="IW774_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1154569.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2719..3831)
                     /locus_tag="IW774_002238"
     CDS             complement(2719..3831)
                     /locus_tag="IW774_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1154570.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(3906..5279)
                     /locus_tag="IW774_002239"
     CDS             complement(3906..5279)
                     /locus_tag="IW774_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1154571.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5500..>6073
                     /locus_tag="IW774_002240"
     CDS             5500..>6073
                     /locus_tag="IW774_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154572.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAP"
BASE COUNT         2044 a         1197 c         1206 g         1626 t
ORIGIN      
        1 aatctgcgtt tttcattcaa aaataaaaaa aacatagaaa aacgattact ttgcttatac
       61 aacaaggctt ttatggtttt gtatcaaaaa aatatttgtt taaatgttac aaatattaaa
      121 taattcgtta ttttgtcatt aaatgtcatg tttcttttac ctatgaaata tttctaaatg
      181 ataaagttaa tagcgtcgaa agaataaaaa acggagaaaa aaaggagttt aatatgaatt
      241 catttaagaa aattgtttta ggtacaactt ttgctgctgg tgctactgca atgtttgtag
      301 gaactacaaa tgctcacgct gatgaagttt atacagtaaa atcaggtgat tcattatcta
      361 agatctcaca aaaatttgca ggagacaact caatgatcga tgcaattgct gaaaaaaatt
      421 cgatcgcaaa catcaaccgc atttatgttg gtgaacaatt aacaatccca acttcaaatg
      481 attcttcagc aacaacagaa aacaaaacaa cagaaaatac agcatctaca actgaaacag
      541 caacgcaaga acatacatac gttgcacctg ttgaaacagt tgaagtagct cctgctgccc
      601 cagctgctgc aacagcgcca acttcaagct ctgcaaaaga atggatcgca caaaaagaat
      661 caggcggttc atacacagct actaacggac gttacatcgg ccgttatcaa ttagatgctt
      721 catacttaaa cggcgactat tcagctgcca atcaagaacg tgtagcagaa caatatgtaa
      781 catctcgtta tggttcatgg gatgctgcta aaacattctg gttagcaaac ggctggtact
      841 aagccgataa aaggaaaatg atgaaaccga gaaacttgtt tattcaagtt ctcggttttt
      901 ttgtgtgaaa aataactcgt ttgcttccca cagcgcagca gtacaaataa aaactcgaat
      961 aatgtataat aaagaaaacg tttttttgtt tagggaatga ggaggagcca tgagtacaaa
     1021 agagagtctc gaattaaacg aaatcacaag tggaaatcca ctgttatcca gtattcgatc
     1081 gattgtagaa acgacatttt acggtaataa tgttcatgaa gtttttgata ggaagacagc
     1141 ttatcaacta gcaaaaggga gtccaggaac gatcatcaca gatttaacaa tctcacatgc
     1201 tgaagaacta gatttaccag ctgatgttag gacactagta ttcaatgatg gaagcattgt
     1261 gggaagaaca gcaagtgctc ggcggatctt tgaggactta gacaaggaac aaagcaaata
     1321 cgagaaaatt ttaagagaag ctgtgtatca aagtcgcaag cgccagtttt accatacaaa
     1381 agtaatcgtt ggcctatctg aagaattttc tgtgcaaagt catttgctga ttccagaagg
     1441 atttgagaac aatttgtatt cttatttgct gaattttcag ccagcgaatt cgaagatctt
     1501 agagacttat caaaagtctg tttcctttga tgagggagat ctttatatct tttgtgatcc
     1561 cgaatggaca catgaaacat ttccaaatgg actcattttg tttgatgctc tccataatac
     1621 agcggttgtg ctaggattac gttactttgg agaattaaaa aaagcgactt tgacgcttgc
     1681 ttgggcattg ggaaatcgca atggctttat tgcctgccac ggtgggatga aacaatatgc
     1741 tagtaatgac cagacctata cgatggctgt atttgggttg tccggttctg gaaaatcaac
     1801 gattacctta gcaaaacata cggaaggtca ctctgtttcg atcttgcatg acgatgcttt
     1861 cgttatttct cgaaaaaatg gttttgctac tgccttggaa ccttcttact ttgataaaac
     1921 acaagactat ccgtctgatg atccggccat ttcttctttc cttacttgtc agaatgtggg
     1981 cgtgacatgt acaagtaaag gagaaaaagt cctagtaaca ggagatatac gaaacggaaa
     2041 tgggcggaca gtcaaatcac gttatgcgac acccgaccga gtcgatcatc ttagcgaaaa
     2101 gatcgatgct gttttttgga tcatgaaaga tgattcgctt ccgccaatta tcaagatcaa
     2161 tgacccagtt ttagcagcgt cttttggtgc aacattagca acaaaacgat cagacgcgga
     2221 aaatatcact acaaaaagac agctagatga gctagtcatt gaaccatttg ctaatccttt
     2281 ccgtgtctat cctcttaggg aagactatca agcttttcga gagttgtttt tgaaacagca
     2341 aacagcctgc tatgtcctga atactggata tttcgatgat aaaaaaatca aacctgaaac
     2401 gacattgtca atcgttgaaa gaatcgtgca acaaacggca gaattcaagt cgtttggtcc
     2461 atttgaagac atccactatt tacctatacc agattttcct gtttcttttg aggatacgtc
     2521 ctataaaaat ctggtgaaac aacgacttga tattcgttta cgattcattg aagctcagat
     2581 aggaaaagat ctcttgccag atgaagcaaa agaaagtttg gagaatttga ttcagaaatt
     2641 gaactaaaat aacagctttt tcaagaagta atccatgcgc aatatcgctc gggatcctta
     2701 cttgaaaaag ctgtttttct atgatggatt tttcgtaata taggtggaga tgtccttttg
     2761 ataatcttcc gtaggccaat gaccagtatt taagaagttg gctaatagat agccgatata
     2821 aggacctgtg gtcaagccag aagagcctag accgctagca gccagtaagc ttggatcatc
     2881 tggcagtacg ccgaaaaatg gggaaaaatc tgacgtataa gcacgggtac ctacacggaa
     2941 ctcgtatggc cagtttttta tctgatctgg atcagtcaag aactgttctg tactcttagt
     3001 caatccctta taagcttcgc tagtaggagc caaatcccag ccttgatcgt tttcatgggt
     3061 agcacctaaa aggatctttc catccatgaa aggaatgaaa tcagcttctc cgtcaagcat
     3121 agcaacaggc cagtcttgac tcatcttata agatgtctga aatgctaaga gctgcccttt
     3181 ttgcggacgt atatctgcag catagcctaa ttcttgtaat aaaggcttaa gtgccgggcc
     3241 aggtgtcaaa attacacgat cagctgtgta ttctccaaac ggtcccttga tgagccaaga
     3301 atcgctcttc ttatgcaaag tgacttttgc ctggatctgc tccatttgtt tttgtgctaa
     3361 acgctgacca agatgttcta aatatgcttt tccatccaga cgaccaccac cactgatacg
     3421 cagtgaaact gctggtttca ataacggaag tgcttgagat gtctgttcag gtgaaagcca
     3481 ttcgatgttt ccaatttctg gcgcttcttc ttttcgtttc tcggcaagaa cagccaattc
     3541 agctaaatct ttttcaggtc gaagcaaaag tgtccctgat tgtgcgtaaa tcgtttgatc
     3601 aagttggaaa tcttttataa gtttagggaa aaaagcagcg ccgtctttag ccagacgata
     3661 ccattgtttg tttctgcgtt tcgacaacca aggggagata atcccagcgc tagctttagt
     3721 tgcctgtcct gtcccttcat caaataaagc aacattgaat tttgatgtat ctaagtaact
     3781 ggctagggtc ataccaatga tcccgccacc gataatggca attttttcca taaaaaagcc
     3841 tccttttaca aacaaccatt ttcacacaaa aaaacggcag atgcaacacc tgccgtatat
     3901 ggaatctacg cttttttagc aaagaacaga taataggcga atcgagcaaa gcttacgaga
     3961 agcaacagca acaagacgcc taatgctgct tttgatccta atgtagtaga cacttcatag
     4021 ttatgggaag tatgatgctg gacgtaattg ataatgaccg ctactaatga agtagccaca
     4081 gcgccagaaa actgctgcaa ggtattgaac attgcgttcc cgtctccttg tagttcttca
     4141 ttcagtgcat tcattccggt ggtcatcata ttgctgtaag ataatccgat accgatcata
     4201 taaaacacat gacaagcaat caatacaccc actgaagcgt gtcctatgag taggaataat
     4261 gctagccaac caatcaaaga aagaatcagc ccagcaaaaa ttggcttctt cggaccatat
     4321 tgatccagca ttttcccgga aaaaggagaa agcaaagcac caaccgctgc gccgggaagc
     4381 atcgctaatc cagcgacaaa aggactactt ccttgaacga tctgaaggaa attcggcaag
     4441 acaaaagata caccaagtaa taaaaactga taaactaaga agccaatcaa aaacaagcgg
     4501 aatgttttat ttgaaagaat agacagtcgg atcagtggat gctccacttt tttacttcga
     4561 tagtaaaaaa ggaaccagcc aataccgccg atgattaaag caatccagct ggaaactgtt
     4621 cccaacgtac tcaaaaatgt tagacttcca ctaaacaaaa gacaaattcc taaaacgctt
     4681 atccaatcca aataaccact tttttgtact gccatctcgg gaatagcaaa ccagccgata
     4741 atcaaagaaa gaatcaaaac gggcacaaga aacaggaaga tatgattcca tgtaaaatgt
     4801 gaagtcataa tgcctccata agtggggcca attgctggag caatggccgt tgtcaaagta
     4861 ccgacaccca tcatcgcgcc tcgtcgattg aggggggcaa atgtcaagat aatatggaac
     4921 atcaacggaa gagcgatacc tgtcgctgca ccttggagca atcgaccaaa caataggacc
     4981 atgaaggtag gagagaagaa atcgatcatt atacctgcca agaaaagcag atttgaagct
     5041 aaaaagagtt ttttgagact aaaattgcga ttcaaataag tagacagcgg aacaatgatc
     5101 gaaatcatca aaagataaat cgttgttacc cattggactt gtgcagtcgt taaatcaaac
     5161 tgtctgataa gagttgggaa agttacattc atggccgtct cgatcaatac gcccgcaaaa
     5221 gacatcatcc ctgttgcaat tactgcaggt actaaaagca gctgtttttg ttttgtcatt
     5281 tacatcacct ctaaaaaatt tcgtaaaaaa agagagtgat ttctattata aaaatagaaa
     5341 ccgcctctct ctcgacacgc cgaaacgtgt tatctgtacg atttatttac ttcacttatt
     5401 ctagcacaaa ctgaaaaata gtgtcaacac gattagccaa gtcactcaaa aagaggtatg
     5461 ataataaata actaaatgga atttcgcgga ggaagaaaaa tggaaaaaat tgaatggcgt
     5521 aaagacaaga gctatagtat gaaaaaaagc ccgcaaatac tagaggttcc taaaaaatca
     5581 ttctttagca tcagagggat cggcgacccg aatcaggaag actttaaacg aagagtagcc
     5641 tgtctatatg cagtcagcta tgcaatacgc atgtcgccta aaaaaaattg gctgattcct
     5701 gattatcaga tgtatactgt ctatccactg gaaggacaat ggggactgca agaaaagtat
     5761 ttgcatgaac cagtgatgaa aaaagaacat ttttcttatc aattgatgat ccgacagcct
     5821 gactttgtta cagaagcgat agcacaagaa gccattcagc gttctagcaa acttccggaa
     5881 gacttgaaag aacaagtagt attcaaaaag atggaagaag gtctttgtgc ccaaatctta
     5941 cacgtcgggc cctatgccga agaaccagca acgtttgaga aattagaagc attcatcgca
     6001 aaagaaggct atgaaagagc ttctaaagaa cacaaagaga tttacatcag cgatccaaga
     6061 aaatcagcac ctg
//
LOCUS       DACEOE010000148         6049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEOE010000148 DACEOE010000000
VERSION     DACEOE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.151"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..226
                     /locus_tag="IW774_002241"
                     /pseudo
     CDS             1..226
                     /locus_tag="IW774_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            352..1323
                     /locus_tag="IW774_002242"
     CDS             352..1323
                     /locus_tag="IW774_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1154573.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            1463..2776
                     /locus_tag="IW774_002243"
     CDS             1463..2776
                     /locus_tag="IW774_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1154574.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            2819..3685
                     /locus_tag="IW774_002244"
     CDS             2819..3685
                     /locus_tag="IW774_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1154575.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            3682..4530
                     /locus_tag="IW774_002245"
     CDS             3682..4530
                     /locus_tag="IW774_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1154576.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            4544..6016
                     /locus_tag="IW774_002246"
     CDS             4544..6016
                     /locus_tag="IW774_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ1154577.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
BASE COUNT         1889 a         1023 c         1311 g         1826 t
ORIGIN      
        1 tcttgaaaaa ttaattaaag acttagataa agccattgac gacttagtcg tggtgctacc
       61 ggagtatcaa tgtttaacca gtatcccagg tattggtcct gtttaggctg ctggaatact
      121 cgctgaaatt ggtccaattg aacgtttttc tgatgaaata aaattgacaa agtatgccgg
      181 cctatattgg cgacagagtc aatccggtaa atctgaatac gaaaattttt ttacgaaaag
      241 cttcacgtct aatatctatt ttaaaataaa aatattcctg ctaatttgga atcgtttcct
      301 tcttttggta taataatacc tgaaattctt ttgagaggag ttgttcgtga agtgaaaact
      361 actatgaagg atgttgctgc gttagcagga gttggtgtgg gtacggtatc tagagtaata
      421 aatggagtaa aggtgaaaga ggttaccaaa gaaaaagtaa tggctgcaat tagtgagctt
      481 aattatgagc ctgatgaata tgccagaggt atgaaaacga atagtagcaa tacaatagct
      541 cttattttac ctacgatttg gcatcccttc ttttccgagt ttgcctacta tgtagaggaa
      601 gctctcagcc aaaaaaatta taagcttttt ttgtgtaatg cgaacggcag tgcggaaaaa
      661 gaaaaagaat atatccaaat ggtcaagcag agtaaagtag acggaattat aggtattacc
      721 tattcagaca ttgataaata tgtatcatca aatttaccat ttgtcagtat agaccgccat
      781 ttttcggaac aggtttctta tgtaacggca gataattatt taggtgggca aattgccgct
      841 caagaactac ttactcgtaa ctgtaaaaac ttgtgctatg ttgggggtgt ttcaccttac
      901 cctaacgaaa caaacaatcg taaagctggt ttttacaata tttgtaagga aaataaaata
      961 gctgtgaagg tattggatat gcctgaacca attagcaatt tagaggaaca attgctcgct
     1021 tttttttcgg ataatcctga aattgatagc atttttacca tcaatgattt catggctttg
     1081 gatgtgataa aagcccaaaa aaaactaggg aaggaagcac ctaaggattt tcaaattatt
     1141 ggttttgatg gagtcaagct atcgttgcaa aaagactatt tactatcaac gattgtacag
     1201 ccgatagagg aaatggccaa ggtttcagta gaaacactca tggcgatgat tttaaatgaa
     1261 gaaccgccta agcgtgtggt agtaccggtt ttctttcacg ctggagagac gactaaagag
     1321 taaaaaaccg gtttataacg atttttttga gataggttga cagaaagtga tttcaatgat
     1381 atcttaaaac caattaattg gaaacgttac caattaattg aaaaataaaa cggaaacgtt
     1441 tcaaaaaata ggaggaacat cgatgaaaaa gagaatgtta gcaattgcag caattttagc
     1501 attaacagct ggaggacttg caggatgtgg cagtaaggag aaaagttcag gtagcagtga
     1561 tccaggagaa attaagattt gggttcagtt ttctgatgaa acgcctgaag gaaaagcttg
     1621 ggcagagatt gtgaacaatt ttaatgatga atacgagggt gaatataaag ctgttactga
     1681 gtatattcct cgtagcggaa gcggcggtgg atatgaggat aaagtaaatg ctgcaattac
     1741 aaccaatacc cttcctgatg ttattacgtt agatggaccg aatactgcag cttatgctca
     1801 ttcaggagtc attgctccat tagatgatta tttgaaagat gcggacatgg atgatgtatt
     1861 agacagcatt attcagcaag gaacctacga tggcaaaatg tatgcgtttg gtttctcaga
     1921 gtccagtgtg ggtgtctatt acaataagtc gctgttcaaa gatgctggaa ttacagaaga
     1981 tcaattgccg acgttagaag atccttggac ttggacggaa tttcaagcaa tttgccaaaa
     2041 gctgactgat aaatacaaaa cgcctgcaat tgatatgcaa atggcgtcgg gagacgaaat
     2101 gctgacctac gcatatacac catttatatg gtcgaatggc ggcgatgtcg ttaatgctga
     2161 tggaaccaaa gccgaagact attttaacgg taaaaaatcc gttgaagcgt tggaatttat
     2221 tcaagaattg gttgaaaaag gttacaccac aatcagtcct attgaaaaag gatttgaaac
     2281 tggcaaatat gccatgatgc tgagcggttc ttggacaatg gcagatttag aggaaaacta
     2341 tcaagagatt gattatggta ttttgcctta ccccgtttca gatgcaactg gggagttagt
     2401 ttcaccaacc ggaagctggc agttagcaat gactgagaaa acagagaaaa aggatgcttc
     2461 tgcggccttt attaagtttg caaccagtac agaatctagt cgtattatga gtttagggaa
     2521 cagtgttcta cctatcagaa attcaacagt tgatttaatt aaagatgaag tgtcggaagg
     2581 aatgcaggta ttaattaagc aaaaccaaat ttctggacat gcccgcccag tggttgtagc
     2641 ttatccgcaa gtatctagag cattccaaca agcagttcaa gatattagct acttcgagga
     2701 aaacccagat gtacaaaaag tagcagatag tcgcgctgct gaaatgcaac aggcaattga
     2761 tacttcacta aaatagaagt ttctaaaggg ggccaatcaa gtgttaaaaa aaccgacaat
     2821 gaaagacaat ttgataggtt acggctttct agcgccagct ttgctgctgc tgacgatttt
     2881 tctgatcata cccgtaatca tggtaattta ttatgccttt actgattatt atttgttaat
     2941 gccagatgct agacaattta tcggcttaga aaattttaag cgactgttcg ctgatccaat
     3001 ttttgtaaaa agtatttgga acacggctaa atttgttgtt tggattattc cggtccaaat
     3061 aggggcagct ttaggaatgg cacttattat caacaaaaat cgtaaaggaa atatcttttt
     3121 taaagtagcc ttttttgcgc cagtcgtgat gtccttagtc gttatttcga ttttatggtt
     3181 gtacttgtta aatcctaatg aaggtctggt taatacgcta tttgcaaaga ttggaattgc
     3241 ttcgcagcct tttttgacta gtcccaaaca agcaatgtat acgcttgtgt tcgtatcggc
     3301 atggcaaggt gcaggatatc agatgctgct gtttttaggg ggaatgcaaa atattccgca
     3361 agacgtttat gaggcagccg aaattgatgg tttttctaag tttcagcaat ttcgctatat
     3421 caccatgcca ttattaaagc ccacggcgat ttttatttta cttaccacat tgatttcggc
     3481 ctttaaacta atcattcagc caatggtaat gactcaaggt ggaccaatga attctactat
     3541 gacaatggtt tattacattt accaaactgg atttacagac cgtatggtag gctactccag
     3601 ctccattgcg ttaatcttta ccactatgat tggtttaatt acaattgctc aaagaaaatt
     3661 ggtaaaggag gatgatccag catgaccaag aaatttggac cgctaatgat cgtagaatat
     3721 tgtttgctgt tccttttagc ggcattattc attttcccga tgctgtggat ggtagtttcc
     3781 tctatgaagc ctgaagcaga tgtctatcaa aatttagctt ccttcaaagc ttttttacct
     3841 agtttgaatc cagctaattg gtttaaaact tatcaagagg ttatttcccg gttcagtgtg
     3901 ggaacttatt tattaaacag tattttttat ggtttaacct ttgcctgtgg gtcaatcatt
     3961 gtaaactctt tggcgggttt tgcctttgcg aaggttaatt tttcaggcaa gaaaattatc
     4021 tttggagctt tgttagctct gctgattgtc ccgatggaga cggttttaat tccgcaattt
     4081 accatcgtta attcactagg cttagtgaat actcgcttgg cagtgatttt accagcgatg
     4141 gccagcgcgt ttaatatcta tctgtttaga aatttcttta tcgcgattcc agaagaaatc
     4201 attgaatcag cgaaattaga tggtgcaaat attgttaaaa ttttctttca catcatgctg
     4261 ccaatgtcta aaccagccat agccacagta ggtgtcttat cctttatctc cagctggaat
     4321 gattatattt ggccgttaat ggtcttaact gacagcagca aattttcaat gcaagtggca
     4381 attactacga tcaatactac acagccagtt tatatcaatc aagtgatggc tgttttaacc
     4441 attgcaacga ttccactgat tattgtttat atcgttgcac aaaaatatat tctgcaggga
     4501 cttggcggtt ctggaacggg tataaaatag gagtgaatag tgaatgttgc aaaaactttt
     4561 aaaggccaat gagtttattg agaaaaatgc tcaaacagtc aatcaagaat accggaataa
     4621 atttcatttg atggctccga ttggctggat gaatgatcca aatggttttg tttattatca
     4681 agaggagtat cacttgtttt atcaatatta tccttacgac agcatctggg gaccaatgca
     4741 ttggggccat gctaaaagca aggatctaat tcactgggag catttgccag tagcactggc
     4801 accaggtgaa agctatgaca aggaaggctg tttttccggc agtgccatag aaaaggatgg
     4861 caagctgtat ttgatttata caggacacaa tgttgtggaa gggcaagttc gacaagttca
     4921 gtgtttggca gtttcagaag atggcattca ctttgaaaaa tatgccggaa atccaatcat
     4981 cgcagaagat cacttagctt ctgttgcaac gaccgaagat ttccgcgatc ccaaagtgtt
     5041 tcagcgaaaa gaagactatt ttactgtagt cgctactaaa actgcagatg ctcgtggaca
     5101 aattgtgatg ttccattcca aagatttact aacttgggag ttttattccg tattgctgga
     5161 gggaactcca gaacaaggca ttgtatggga atgcccagac ttgtttgaac tagatggaaa
     5221 agaagtcttg attctttctc ctattcaaat gactccacaa ggcaatgctt accaaaatac
     5281 gaactcaact gttgctttca tcggtactgt cgattggcaa acaggcaaat ttctagtaga
     5341 gaattttcat gaaatcgatg gcggtttaga tttttatgca ccccagacct gtgaaaacga
     5401 agcaggccaa agaataatga ttgcttggat gcaaatgtgg cagcgtaaaa tacctacgaa
     5461 tgatctgggc catggttggt caggttccat gaccttgcct agagagctga gggtggctga
     5521 taatcaattg ttgcaacggc cagtttcatc aatttatcaa gtaattgatc cgcaggaaag
     5581 gattgaatgg gcgaagactg aagagaatcg cttcttactg aaggacttag tatatgacca
     5641 gcactattta cgcttggaaa tcaatttaaa acaggcaacg aaactagaac ttcactatgc
     5701 gaagggaccc gaccatacat tactgcttaa ctatgatgtt gaacgggagt gctttactgc
     5761 ttccagggaa cacatgccgt atgatttatc aggagctgaa aaggaagtgt tgaaaaagcg
     5821 cagtgtgacg attcctttgg aaaataattg tctaaccatc gaaattttcc gtgatacttc
     5881 agctattgag ctttttttaa atgatcgtct gactatgagt acaacttttt atgagacaga
     5941 aaaaggacag gattttcttt tgctggcaga cagtgaagta accttttcat tggaaagcgg
     6001 cttaattaat ctatagaagg gagagctcag tgatgattta tggttcaat
//
LOCUS       DACEOE010000149         5998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEOE010000149 DACEOE010000000
VERSION     DACEOE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.152"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            279..803
                     /gene="infC"
                     /locus_tag="IW774_002247"
     CDS             279..803
                     /gene="infC"
                     /locus_tag="IW774_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1154578.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            875..1075
                     /gene="rpmI"
                     /locus_tag="IW774_002248"
     CDS             875..1075
                     /gene="rpmI"
                     /locus_tag="IW774_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1154579.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1128..1487
                     /gene="rplT"
                     /locus_tag="IW774_002249"
     CDS             1128..1487
                     /gene="rplT"
                     /locus_tag="IW774_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1154580.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1739..3046
                     /locus_tag="IW774_002250"
     CDS             1739..3046
                     /locus_tag="IW774_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1154581.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAIFA"
     gene            3123..5252
                     /locus_tag="IW774_002251"
     CDS             3123..5252
                     /locus_tag="IW774_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1154582.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKQQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            5227..5916
                     /locus_tag="IW774_002252"
     CDS             5227..5916
                     /locus_tag="IW774_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154583.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
BASE COUNT         1937 a         1072 c         1328 g         1661 t
ORIGIN      
        1 gaatcactgc tagaaagtct gctaatctaa tcattttcag aaaaggaaga caaactgtat
       61 gaaaaacatt gacaaaaaga ttagtttttg ctatactatg atggttgaga aataaaagca
      121 gaagcacccg cttctcgcct tagttgataa tgtcactggg ctagatagtt ttcactggtt
      181 ctattaggtg aagtacgcgc gggttctgta agagaactcg tttttttatt gccgtttatt
      241 cggtaagctt ttctttctct cataatattt ggaggtgaat gaccatagca aaggatatga
      301 tggttaacga cggcattcgt gcacgagagt tacgattgat cggttcagat ggtgagcaat
      361 taggagttaa gacaaaagca gaagcattga atattgcaga acaagcaaac ttggatcttg
      421 tgttagttgc ccctggtgcg aaaccaccag ttgcgcgaat catggactac ggtaaatacc
      481 gtttcgaaca acaaaagaaa gaccgcgaag cgcgtaaaaa acaaaaagtg atcaatgtaa
      541 aagaagttcg cttaagtcct acgatcgact tgaatgactt caatacaaaa cttcgtaatg
      601 cacgtaagtt cttggaaaag ggcgataaag tgaaagcttc tatccgtttc aaaggccgtg
      661 ccattaccca taaagaaatt ggtcaaaaag tcttgaatcg cttagctgaa gaaactgcgg
      721 atattgcaac agtggaacaa aaagcgaaaa tggacggacg cagcatgttc ttgacgctgg
      781 caccgaaaaa cgacagtaag taatttgaat gactaactgg ctgttggaca gccgatagtc
      841 ggataacgaa agattttgag gaggaaatat agtcatgcca aaacaaaaaa cacaccgcgg
      901 atcagcaaaa cgtttcaaac gtactggtaa aggcggattg aaacgcttcc gcgcgtttac
      961 aagtcaccgt ttccacggca aaacaaaaaa acaacgccgt caattgcgta aagcaagcat
     1021 ggtttctagt ggcgacttca aacgtattcg ccaacaacta gcaaaaatga aataagaagt
     1081 acaaataaca gaatctgaag atttattttt attaggagga attatacatg gcacgtgtta
     1141 aaggtggaac agtaactcgt aaacgtcgta aaagagtgct aaaattagca aaaggttact
     1201 acggttcaaa acacacttta tttaaaacag caaaagaaca agtaatgaat tcatacaact
     1261 acgcatatcg cgatcgtcgt caaaagaaac gcgacttccg taaattgtgg attgctcgta
     1321 tcaacgcagc agctcgtatg aatggcttga gctactcaaa attaatgcac ggtttgaaag
     1381 ttgctgaaat cgacatcaac cgtaaaatgt tagctgactt ggctgttaac gatgcagcag
     1441 catttactgc attggctgac caagcaaaag aagctttggc taaataagct aaacaattat
     1501 aaagaggaaa gaagataacg aaaagttatg ttctttcctc tttttttatt tacaatcctc
     1561 catcaaatga aacaggacaa taacgtataa gtctaaaata gcataattaa ggttttttaa
     1621 ttgaaaactg aacatttatg gtcacccttt aatgaaagcg ctaactacat aagttagaat
     1681 gagtttgact gatatattag tttgcttatc agaaaaacaa aatggacgga ggaataacat
     1741 gagtgatatt attattggta ttttgttagt tatctcattc tttttcatgg tctggtactg
     1801 tgtaaaaggc tacaacttga tggtcggctt tgccatcatg gctactgtat ggatgggatt
     1861 agcgctagtc ggaaacacgt tttcaccaaa cccggcgatg gaagggcaag gagtaattga
     1921 tgtgttgacg catatctata cgacggggcc agctgagtac gccaaatcga tcttggtaaa
     1981 tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat agcggaatcg cagctaccct
     2041 gattcgaaag gtagttgaac ttggagggga caagccaaga atcacgatga gcttactatg
     2101 tattgtaacc gctgttatat tcatgtcgat gacaggaatc ggaccagtga tttctattgc
     2161 agttattgtg ttgccaatct tgatgtcatt aggtatttca gtaccggttg cactgttctc
     2221 atttatgggc tcgattatgg ctggaatctt tgctaatatc gtcaatttca aacaatatca
     2281 aacgatttat gctggtttca atccagcggc tgaaagctat acttataatg actatttcca
     2341 aattggtatg attgggatga ttgtaagctt agttgtggta ctaacggtcg ctaatatatc
     2401 aatgaacaaa aagaaacgct atgcaatggc agcaaacgta ccagctgaag gtggcgacgc
     2461 accaatgatc tcatggttag ccgtattatt accagtttta ggggtcgttc ttttagatct
     2521 tccaatcatc ttaggtttta ttctagcagg tatctgggca ttgctgttta ctggaaaatt
     2581 gcgcggaggt tataaagaaa tctgtcgtca gtttgcgaaa ttatttacag atggcgcagt
     2641 agatgttgca ccaatggttg gtttcttgat gaccttagcg atgttcaata attcagctgc
     2701 gtatgcttct ccttattttt cagctatttt tggagattgg ataccgcaat caccattagt
     2761 cttggcaatc gtatttgcta tattgacacc actaggattt ttccgcggtc caatgaatct
     2821 agtcggttca ggctcagcta ttttagctgt cgtattagct gttaatccaa cgatgtcgcc
     2881 agcctttttg ttcccgttat ttgcaatcac aacgattgct cctcagcatt tagatattac
     2941 acaatcttgg gttgcttggg gattaggtta tacaaaagta acttctcgag aatatatgaa
     3001 gaaatccatc ccaactggtt ggatcatcgg agcgatcttt gcctgatcac gttcttattg
     3061 tacgggaacg cctagtaaac aaaacgtcga taaaaacgat tagatgaatg gaggaaaaga
     3121 aaatgaaacg ttttgtgcga atgggaattg atgttggcgg tacacatacg aaagcagtcg
     3181 caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc agtgaaaaca acccacgatg
     3241 atgtccgtgg agtagccgca ggtgtcgttc agtcttttca aaattgtttg cgggaaaata
     3301 atatcagtcc agaagatgtt gtgtttgttg cacattctac cacacaagcg actaatgcac
     3361 tgattgaagg agacgtagca aaagtcggcg taatcgggat ggcaaaaggt ggattagaag
     3421 gttttttagc aaaacaacaa actagattga acgatatcga tttagggaat aagaaaaaaa
     3481 tagagattgt caatgcattc ttacctgtca aacacttaaa tgtagaccgt gtttcagaaa
     3541 cgatttcttc tttagaaaga gaaagagcgg aggtcttggt ttcttctatg gcatttggag
     3601 tagataatgg ggaaccagaa cgagttgttt atgaagcagc atcggtaaaa tcgatcccta
     3661 caacgatggc ttctgacatc acaaaattat atggattgac aagaagaact cggacagcag
     3721 caatcaatgc atctatttta ccaaaaatgc tagatacggc aacctctaca gaagattccg
     3781 tacgagaagc aggggtcaat gtttctttga tgattatgcg cggagacggc ggagtaatgg
     3841 aaatcaatga aatgaaaaaa cgacctgttt taaccatgct ttccggtcca gcagcatcag
     3901 tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt ttattttgaa gtaggcggaa
     3961 caacaaccaa tatcggggtc attaaaaatg gacgtccagc aatcgattat tcaattgtag
     4021 gtggccaccc aacatatatc tcttcattag atgttcgggt tctaggtgtg gcaggcggtt
     4081 cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg tccacgttct gcacatattg
     4141 ccggtctaga ttatgcagta tttacggaga cagaaaaaat caaaggtcct aaggttgaat
     4201 ttttctctcc taaagaagga gaccctgctg attacgtcaa ggtagtgatg gaagacggag
     4261 aagaagtgac tattacaaat acatgtgcag ccaatgtcct tggattagtg caagaagaac
     4321 atttttctta tggaaatgtc ccatctgcac gaaaagccat ccaagcatta gctgattatt
     4381 gtcatacgac ggttgaagac atcgcagaac agatcatgga aaaatcctat gctaagattg
     4441 aaccggtgat tttagaattg gcagacaagt atcacttgga gaaagatcag atttctcttg
     4501 taggtgtagg gggcggcgct gcctcattga ttacttactt tagtaataaa atgggtgtga
     4561 aatattcgat tcctgaaaac gccgaagtga tttcttctat tggtgtagca cttgcaatgg
     4621 tccgagatgt tgtagaacgt atcatccctt cgccaagtaa agaagatata cgatcgttga
     4681 aaaatgaagc gatgaataaa gcgattgaat caggcgctac accagaatcg atcgaagtcc
     4741 acgtagaaat cgatccacag acttcaaaag taacggctat cgctactggt tctacggaag
     4801 tgaaagcaac cgatctaacc aaggagatca caacagaaga agcactagag cttgctgcag
     4861 aggatatgcg attaaataaa aatgaagttt gcttattgga aaatacacct ttcttttatg
     4921 tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct tcggatcatc gatcaaaaag
     4981 gatttatcaa ggtccaacgt ggacatgctt catgtatgaa aacaactgca gccaattata
     5041 tgacagctgt cgaacaactt tgggaagata tggcagttta tcaaactgaa ttgattgccc
     5101 gaccagaatt ttatctctgc ttaggagcaa gagtctctga ttttacagcg acggatctgg
     5161 aacaactaca gcttttgatg gatctggaag tatctactat ggaaccggag gaagaagtga
     5221 tcgtcgttgc cggaaatatc aaacaaacat aagttagaag agttactaaa cgaattacaa
     5281 cagatccccg aagaaatttg gggattctac cagtttcggc gagatttatt ttggaaaaag
     5341 attcctttgt ccaagcagaa aattcttata caacaatcaa tcgattgtgg tatcgaaacg
     5401 gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag gagaaatttg tgaacaaatg
     5461 gctgttccaa tcgtttcctg tgaatcagag caaataaacg aacgaatcac ttttgcaact
     5521 tatgcagaag atgaagggat tcgattgatg acggagccgt tagaaaaatt aaaatgttca
     5581 ggactagctt cgatctctaa agaaacagcg caagctttga ttattggaca tgaactattt
     5641 caccacatcg aagcttctgt taagggaatt tatacgcaaa gcgaaaaaat tgtgctgtgg
     5701 cggttgcctt tttatacaca tcaatcaaac attcgtgcat tgagcgagat agcagcgatg
     5761 agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt atgtattaga ggcagtctta
     5821 ctttggccat ataatgaaat tcgtagccaa gggatcttag aagaaataaa aaaaatagaa
     5881 aaaagatgta cggagtatga tttagattac aaataaagag actgtgacaa aagtcgtgtc
     5941 acagtccttt ttttccgaat aaacgatgtt ctcctgtcaa ctcctcggtc acaagtca
//
LOCUS       DACEOE010000150         5958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEOE010000150 DACEOE010000000
VERSION     DACEOE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.153"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            184..2277
                     /locus_tag="IW774_002253"
     CDS             184..2277
                     /locus_tag="IW774_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1154584.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            2302..2604
                     /locus_tag="IW774_002254"
     CDS             2302..2604
                     /locus_tag="IW774_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1154585.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            2631..3920
                     /locus_tag="IW774_002255"
     CDS             2631..3920
                     /locus_tag="IW774_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1154586.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            3939..4346
                     /locus_tag="IW774_002256"
     CDS             3939..4346
                     /locus_tag="IW774_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1154587.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            4369..5238
                     /locus_tag="IW774_002257"
     CDS             4369..5238
                     /locus_tag="IW774_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1154588.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            5240..5860
                     /locus_tag="IW774_002258"
     CDS             5240..5860
                     /locus_tag="IW774_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1154589.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
BASE COUNT         2009 a         1023 c         1265 g         1661 t
ORIGIN      
        1 tgtatagatg ggcaaaattt ttgtaataat aggcgtatgt ctttttcgtt gaggagatga
       61 aaaagcatat ggcttatttt ttgtttgctt tgcttacttt ctttccactt caagtggcag
      121 aaatatctac tttttattat gtaagcggaa ccatatactt gaaatcagaa ggagatcatt
      181 tctatgaaaa caagaacaat tgaaatactt cacagaatca tgacagccca atatccagta
      241 aaagcaaaac atctagcaag tgagttcaat gtcagtcagc ggacgatccg tcaggaagtt
      301 cttgaagcga atatctggtt gagcagtaaa aagcttccgg agatccgtac aattcgcaac
      361 aaaggttttt tgttgaagct ggaaaaggaa aagcaagctc gattagaaac tgaaataaaa
      421 gatgtgaaag acggcttttt gaatcggcgc gaacgtattt ttgatctagt tttagccgtt
      481 gcttacagcc gaatcccgat ccatctaaac aaaaaagaag aagaatttca aatctctaaa
      541 agtacattag atgaagatat gagacggtta cgagcaacac tggtcagtta tgggatagag
      601 atcgtcagtt atggaaagca aggactggtt tataaaggtt cagaacgatc tatccgtacg
      661 atgatctatg attttatcaa tcagaatctg ggaatgaccg acttctttat gaatcaagat
      721 gcacaattaa caccggctca aaaaatcttt ctccattatt ttcctttggt ggaaatcaaa
      781 aaaatacagg agatctattc caataagttg ttgaaacaag aagatgatat ttataaaaaa
      841 caaatcctac tttttacatt gatctggatc cagcgaatca ggaaacatga atttatctct
      901 gccattaatt ggaaaaatac agaaataaaa gacagtagct tttctgcatt tattgaccac
      961 atgatcgagg aatttcaatt ggacaatgtc ccgcaagtag aaagaaatta tcttcgtttc
     1021 actattgaga cgttcaatgc tagagatatc tccaattcaa tcgagtgggt acaagctcaa
     1081 ttactaacga tccaattgat ccaatttgta gagaacgaga cacacgtacc tttccatcta
     1141 aaggaagaag ctttattcga aaatctctat aagcacatgg ctgcactgat tgtaagaatc
     1201 aagaatcata tccaagtcat gaatccattg aaagaaaaca tccgaacaaa ttatttccca
     1261 atctaccgcg cagtcactca ttttgttcct acgatcgaag aagtaattgg tggcaagata
     1321 ctggaggacg agatagcttt tcttgtcatc cacttttcga cgatcgccag tgtaatcaag
     1381 cgagacttga attatgttta taaatcagtc gttgtctgta accatgggat ggctactgga
     1441 aatctgttag cagagaatct gaaagaaaag tttcctcaga tcgagattac tgcagtttta
     1501 agttcaaaag aaatcgaatt agtagataaa ttggatgtca atctggtttt cagcaccttt
     1561 caattgaatt atcgaaataa gccgctttta gttgtcgatc cgattttaac tgagacgaat
     1621 atcttggtta tagaagactt tttgaaaaga aatgaagaac tacagcggtt agagcctaac
     1681 agtcatgaat cgacggagtt attcactcaa ataatagatg tcatgaaaga aagtggtggc
     1741 cgagtagatc gaatcatcta tggcaagtta gaaaaattat tcgaaacaaa caatttagaa
     1801 atcaacaaaa gggagatcca gcctatgtta aaagatattt tgacggacag caatatcatg
     1861 atcaaagaaa aagccactac ttgggaggaa tcgattgaac tagctgctag accattggtt
     1921 aaagaaaaca ttgtcgaaga gcgctatgtc gaagcgatga ttcgtgcagt gaatgattac
     1981 ggaccgtata tcgtgattgg caaacacatg gcactagccc atgcccgacc agaagatggg
     2041 gtcaataaat taggagtcag cgtagctacg attgagcaac caatcgattt cggtaatcct
     2101 gaaatggatc cagtaaagat tattttttgt ttggcagcag tagactccta ctcgcatctg
     2161 aacatcatga aagaactaat tgaattgatc aacaacgaag agaagttgga tcgattgatt
     2221 gaatgcacaa atatcgaaga tttcaaacaa ctgctttttt cagtagttga gaaataataa
     2281 atgatacggg ggagaacaaa gatgaacaaa ttgaacattt tattcgtatg cggtgcaggt
     2341 ttaggaagta gttttgcagc acagatgtct gctgaggatg ttttgaacaa attaggagta
     2401 gaagccaaac tggaccattg tgatatttct tccgcagttt ccatgaaacc agatgtgatc
     2461 atcacagcag aaaattttcg ttcgcagttc gaaaagttcg cagttgatcc taaaacggcg
     2521 atgatctatt tgaaaaatat tgtttcaaaa aatgaaattg aagaaaaaat caccccagtt
     2581 ctgcaagata agggagttat ctaaatacaa aaagaaaaga gggtaaaaac atgggcgtta
     2641 tcaattttat catcgaaaat attttgactc aggcatcgat caccattgca ttgattgcta
     2701 tgttgggtct actactgcag aagaaatcag caggacaagt catctctggc acgttaaaga
     2761 cactcctagg atttcaagtg ttgagtgccg gttcgagcat catcgttgga agtttaactt
     2821 atttcgggaa aatctttact gaaggatttc atatgcaagg gatcatccct tctattgaat
     2881 ctatcaatgg tcaagcaatg aatgatttgg gtttaggtag agatattgca ctgacgttct
     2941 tagccatttt tgtattcaat attattttgg ctcgtttcac aaaatggaag tacatttttt
     3001 taactggtca agcaatttta tggatggcaa cgatgacgac agtctttggc tattttgccg
     3061 gtctgcgagg gatcgtattg atcttggtcg gtgattttat cggagcttgt ttcgccattg
     3121 ctatgccggc agtagcgcaa ccgatcattc gtaaaatcac tggatcaaat gatattgctt
     3181 taggtcattt ttgtacgatc gggtacttat ttgaagccgg tgttgctaag ctttttggtg
     3241 agaaaggcga aaataaaaag tcgatcgaag atatcaagtt gccgacacat ttcgaatttc
     3301 tgcaagatac ttatttgtct gtcatggtcg tgatggtccc gctatatatc atcactgttt
     3361 tatttgcagg cgaaccattc gcttctgaat tatcaggaga tcaaaactat atcatgtttg
     3421 cctttttaca agccatccaa ttcgtagtgg gtgtgtatgt attgctagct ggtgtacgtc
     3481 tactgttagg tgaaatcgtg ccggcatttc gagggatagc tatgaaatta gttcctgatg
     3541 cgatccctgc cttagattgt ccagttttct ttccttatag cccaaatgct gtcattttag
     3601 gtttcattac gacaacgatt ggtacgatta ttgccatgtt tactttgccc atgttcggct
     3661 tagcgatgat tttacctggt atgctaacca atttctttgc cggagggaca gcagggatat
     3721 ttggcaacgc agttggcgga cgccgaggag cgatcattgg cggtattgct catggtttct
     3781 ttattacatt attgcccgct ttgctagtaa cgatctttaa ttcgatggga ttcatcaatg
     3841 ctacagcaac cgatgtagat acagtagccg cagcactctt atatgcctgg atcttaagcc
     3901 cagttttgaa agcattttaa ttgaaagtag ggaaaatgat gtttggattt tttaagaaaa
     3961 aagaaaaaga accgaaaaaa gttctggcaa aagaagaaaa agccctatcg ccagaagcga
     4021 ccgaaaagat tcaatcagag attacacaat tgaaacaaga gatttccact acccaagata
     4081 agcataaatt agcgaaactt tatgaacaag ttggacttaa attttctgaa ttgtatgtga
     4141 acgatcaagc catccaatat ttagaaaaga gtttggaaaa taaacagacg atcggagatg
     4201 ggtataaaaa actgatgagc ctctataacc agaagcgtgc agatgctgct cgtgctggtg
     4261 atgatcaagg gatcgattac tacatgggca aaatggatga gatgcgacaa attgcaaaac
     4321 aagtaacgat caaaggaaac aaataaaaag ataaggtggc aataaaagat gtatacaaca
     4381 ttgaaagaag taacaaaaac agcagaagag ttgaatttta cagttggagc ttttaatacg
     4441 cataacctag aaatgcttcc tgctatgcta ggcgcagcaa aagaaatggg ttcgccgatc
     4501 attattcaaa caagtgtcga taccgctaaa tatatcggca tgcatgtgtt agtcaatgta
     4561 atgacagcta ttgcagaaga agaaatggtt gatgcagtct tgcacttaga ccacgcccga
     4621 gactttgatg atatcaaaga agcaatcgac agcggatata cttctgtcat gtatgatggg
     4681 tcccatctgc ctttcaaaga aaatatcctg aagacaaaag cagtagtaga atatgcccat
     4741 gcgcacggtg tttccgtaga aggagaactt ggaacaatcg gcggtacaga agaaggaatc
     4801 catgtcgctg aaaatgacaa ggtatataca agaccggaag acgcagtgaa atttgtagaa
     4861 gctacaggcg tggatgcatt agctattgct atcggtacga atcatggaca attcaaatca
     4921 aaaacagaag taaacattcc cttgctgaaa gaaatcgatg cagtagttga tgtcccccta
     4981 gttatccatg gaggaacagg tgtgaaagaa gaagattatc ctgaattgat caataacggg
     5041 atccgcaaat tcaatgttgg aacggagctt ttagtcaact ggacgaaagt agctaaagaa
     5101 acctttagtg aaacagaaat caacaagtct cttcgccata atgtgattcc agcaaatgaa
     5161 gcagtaaaag aaattgtcaa gcataagatt ggactgttta tgaacttgga aagtccaatt
     5221 aacttaggaa ataaatgaga tgaaaaagta tttcattctt ctggctggta gtcctggtac
     5281 aggaaaaaca tatcttatga ataaactacg ggaacgattt ccagatatgt atgccatcac
     5341 gctcgatgaa atcaaggaat actatgcaga atccatcggg tttaacaatt tggaagaacg
     5401 ggcggaacaa gaaaggacga aagtttatcc tttctattac aaagcacttg atttgtatat
     5461 ggaagcagga aagaaagtga ttgtgtccga gtatcctttt agtgataagc aaaaagcacg
     5521 actccaagaa ttagcagata gatacggata tcaggtgatc acgattcgcc taacagcaga
     5581 ctttgaagtg ttatggcaac gtcggtacaa acgtgatcgt gaatcagatc gtcatttgag
     5641 ctatatcatg gatcattatc actatggtga cgtattggaa gatcgaacga aagggaccaa
     5701 tcatattacg aaagaagcat tcaaaaagat catcgccgac cgaaaatatg acaaattcca
     5761 gttaggaaca ctatatgaat ttgatgtaac agactatact caagtggatt acactccact
     5821 attggataaa ttagaaaaag aaatcaatga caataagtaa caaagaaatg gcttaatatc
     5881 gaaaaattgg cttttgaacg ctgtttaccg agaggtcgtg agcctgtcgt tcagctccga
     5941 gtcacaagga gcaatttc
//
LOCUS       DACEOE010000151         5878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEOE010000151 DACEOE010000000
VERSION     DACEOE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.154"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            642..1376
                     /locus_tag="IW774_002259"
     CDS             642..1376
                     /locus_tag="IW774_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1154590.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1392..2561
                     /locus_tag="IW774_002260"
     CDS             1392..2561
                     /locus_tag="IW774_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1154591.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2577..3572
                     /gene="lacD"
                     /locus_tag="IW774_002261"
     CDS             2577..3572
                     /gene="lacD"
                     /locus_tag="IW774_002261"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1154592.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYAEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3651..4577
                     /locus_tag="IW774_002262"
     CDS             3651..4577
                     /locus_tag="IW774_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1154593.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4596..5846
                     /locus_tag="IW774_002263"
     CDS             4596..5846
                     /locus_tag="IW774_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1154594.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1968 a          956 c         1203 g         1751 t
ORIGIN      
        1 agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt gactaaacga
       61 agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat tcagataaac
      121 tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc ttaagagaga
      181 acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat ttattctcat
      241 agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga tttgaaacca
      301 aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca ctggtaagta
      361 gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta aaaaaagata
      421 ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa aagttggaaa
      481 attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat attaacaaga
      541 ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa ataccactac
      601 tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat acctttcaat
      661 caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa aacagtatgg
      721 tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc gttagtcgca
      781 cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat cgacaacatg
      841 gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct ggcgcatata
      901 gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata ttggccttca
      961 agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta ggtgagcctt
     1021 tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc gagaaatcct
     1081 atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg aaacctttat
     1141 ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag gaattttatg
     1201 ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga tcaccagtgc
     1261 tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt acattaagtg
     1321 tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact aactgaattg
     1381 gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga gcagaaaata
     1441 ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac cgttatcaag
     1501 aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct ggagagcgac
     1561 caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat acactaattc
     1621 cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt ggttcgaccg
     1681 atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc ttggtctcat
     1741 ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc aatcagctgg
     1801 tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg gctacacaaa
     1861 tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat gacgctggtt
     1921 ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt tttgatcaag
     1981 caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga gaagaagtca
     2041 tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc atcatttact
     2101 taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt ctcgagttaa
     2161 ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat ggtcccaaat
     2221 cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca tatacgcaga
     2281 aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa cgcgttgttg
     2341 gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt gcagattcgt
     2401 tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg atcgcattac
     2461 tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga acagtcaatc
     2521 gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg tgaagcatgt
     2581 tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca gaaggcatta
     2641 ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg gctttaggga
     2701 tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa gaattgactc
     2761 cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca cagaagcgtg
     2821 cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa accgtacccg
     2881 gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag ttaggtgcgg
     2941 atgcggtgaa atttttactg tattacgatt atgcagaagc agaggaaatc aatcggcaaa
     3001 agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg ccattttatt
     3061 tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc tatgccaaag
     3121 caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag tataaagtag
     3181 atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat gcaacagggg
     3241 agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc gatgcaacag
     3301 atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg gagacattgc
     3361 gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga gcaacttgga
     3421 aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa tggctgacag
     3481 aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact gccatcccat
     3541 ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt agtgataaga
     3601 gatatgtgtg attataattt atcattattg aaactgggag gatattttat atgaataaaa
     3661 gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa ttaaagcagg
     3721 caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt agctgtcctt
     3781 acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct gatttaattt
     3841 tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta actctaaaag
     3901 agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta aatttagaac
     3961 ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt ggtagaatcg
     4021 tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt tgtttaactg
     4081 gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca ttatccaaat
     4141 caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt gatgaaccaa
     4201 ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt ttattagttc
     4261 ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc gttgattata
     4321 taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta acggctaatg
     4381 gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta gcatcaaaag
     4441 catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt cttccagaaa
     4501 atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt atgttaggaa
     4561 aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt caaaaaatag
     4621 ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg gctgcagtta
     4681 tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg atagaaggat
     4741 ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca tcagattcaa
     4801 catttggtat tattggatta gctgttacct actctttggg acacaatcta gcaaagactt
     4861 atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt ttacttacac
     4921 ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt acctcgggtc
     4981 tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg tttgtactta
     5041 aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga gctttcgttg
     5101 ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta atttgttata
     5161 aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa ccactatctt
     5221 tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt ttcttttgga
     5281 tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta ttattgcaaa
     5341 actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac attataactt
     5401 atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt gcacttgcca
     5461 ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag ttatcaatcg
     5521 ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact atattaaata
     5581 taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt acttattttt
     5641 caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg acaactccac
     5701 cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg attcaagtaa
     5761 taacaattgc aataaatgtt ttgatatact atccattctt taaattagat gataatgcca
     5821 agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt agttaagt
//
LOCUS       DACEOE010000152         5725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEOE010000152 DACEOE010000000
VERSION     DACEOE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.155"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            184..639
                     /gene="tnpA"
                     /locus_tag="IW774_002264"
     CDS             184..639
                     /gene="tnpA"
                     /locus_tag="IW774_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1154595.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            904..1002
                     /locus_tag="IW774_002265"
                     /pseudo
     CDS             904..1002
                     /locus_tag="IW774_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1225..1455)
                     /locus_tag="IW774_002266"
     CDS             complement(1225..1455)
                     /locus_tag="IW774_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1154596.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1685..2503)
                     /locus_tag="IW774_002267"
     CDS             complement(1685..2503)
                     /locus_tag="IW774_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1154597.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            2664..3353
                     /locus_tag="IW774_002268"
     CDS             2664..3353
                     /locus_tag="IW774_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1154598.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            3367..4869
                     /locus_tag="IW774_002269"
     CDS             3367..4869
                     /locus_tag="IW774_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1154599.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4882..5358
                     /locus_tag="IW774_002270"
     CDS             4882..5358
                     /locus_tag="IW774_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1154600.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(5455..5565)
                     /locus_tag="IW774_002271"
                     /pseudo
     CDS             complement(5455..5565)
                     /locus_tag="IW774_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1951 a          837 c         1035 g         1902 t
ORIGIN      
        1 aacttaaaag ttttagaccc ttttattttc ttaatgactt cagtcagttt tgtgcgtaaa
       61 gcgtgcgaaa caagaaaacc gcgcgtttct acgcgtggaa gaagtatctt tagcgataaa
      121 aactcctttc gatatactag tgagtggcta ccaaccgctc atcagatcga aaggagtttt
      181 ttcatgtcaa atgacgataa aagtttagca tatactcgtt ggaattgtaa gtatcatatt
      241 atttttacac caaaatatca acgaaaagta atctatgggc aattacgaaa agatcttgga
      301 aaaatactta ggaagttgtg cgaaatgaaa gatgtggaaa tcatagaagc acatgcaatg
      361 ctagatcata ttcacatgct tgttcgagta ccaccaaaaa tatgtatatc cagttttatg
      421 gggtatctga aaggtcgtag tgcggtacta atccacgagc agcacgcaaa tctcaaatat
      481 aaatacggaa acaaatcctt ttggtcgaag agatactatg tgagtacagt cggattgaat
      541 caaaagacaa atgaaaaata catccgtgaa caagaagctg atgatcgcat tcgatatatt
      601 ataagtaaaa gagagtacgt agaaccattt aaaaaataaa gagaagtaag tgagtgaaga
      661 tgagcagttg gcggtctttt aaaaatcccc ttttaagggg cagcgagtgt cgaccccttc
      721 tagggggatt aaaaatacac ccgtcctcac gggtgtatgg gttactgata gacttgattt
      781 taactggtgt cagaaactta tgtccagaaa gggtgatgaa attttggcaa tttacggcta
      841 ttcaagaatt agcacaagca accaagatta caaaacacaa attcaaacat tagaaaatgc
      901 tggcgcagaa ataatcttcc tagaaaatca ctggattatc gaacaccttt ggaagtattt
      961 ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag agtatattta
     1021 cttcagatgg taaggcaaca aaataaaata caataggcag atgaactaat aaataaaatc
     1081 tgtattaaat acagataaat taatataaaa atcaaaagga tcataaactt ttaacaagtg
     1141 atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg aagtattttg
     1201 caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa caagatgatt
     1261 gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa acattcgctt
     1321 agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt cctgctcatc
     1381 gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga gaggatgatc
     1441 cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt tctttcaagt
     1501 tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg taggaactac
     1561 agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat caagtatgct
     1621 ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc taagtggggg
     1681 tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt aattcaactg
     1741 aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata taaagttgtg
     1801 atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt tgtaaaacta
     1861 agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata atttttgcgc
     1921 catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca gataaagaaa
     1981 aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga aaatgagggt
     2041 ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa tcaagactag
     2101 tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg atcattttaa
     2161 tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga gcttctgtta
     2221 aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt ttttgactaa
     2281 agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca attttttgac
     2341 aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca ctcatcgtac
     2401 tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt tcagacttcg
     2461 ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact cctctacaat
     2521 ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata ctccattttt
     2581 atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc caaaaataat
     2641 aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt atttcaaaag
     2701 gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag gaagaaagtt
     2761 taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata cgtgcaaatt
     2821 ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt ggaattatta
     2881 aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa gtagatgaat
     2941 tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat cgacatgatg
     3001 gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat cagttattta
     3061 tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga atagattttg
     3121 taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg ccagatatag
     3181 atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga aggattcata
     3241 ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt gtgggtggtg
     3301 ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt tagggggaga
     3361 aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc aattgctata
     3421 ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga gagtgctata
     3481 aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat tatgagttat
     3541 gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat tgctgtagga
     3601 cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta tctagtatat
     3661 actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac actagataca
     3721 ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa tcgttataga
     3781 aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat tccgattgtt
     3841 tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc accaattcaa
     3901 aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg aactttttta
     3961 tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat ttaccctttg
     4021 ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat tgctggagcc
     4081 caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt tacttatggt
     4141 actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc agcatgttat
     4201 gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct ttattttagc
     4261 ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat gtttttattt
     4321 gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt ttattttgct
     4381 gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga ctttttatta
     4441 ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc ttttggtatt
     4501 gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt taaggtagct
     4561 attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa caaaaataca
     4621 tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga aaatattgag
     4681 tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga gattattcaa
     4741 aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg tggcatccaa
     4801 gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat attaggaaaa
     4861 gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca agaaactttt
     4921 tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc tgtcttttca
     4981 caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat ttatgctcca
     5041 ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat aaaaagcaac
     5101 tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca aggagagcct
     5161 ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt ggttgatgtg
     5221 gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt ttttccggag
     5281 aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg gaattcatgt
     5341 ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct aaataggata
     5401 tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat ttttttattt
     5461 tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat aaaagcttcg
     5521 aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata aaatatagag
     5581 agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca acttaggaaa
     5641 tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc actatatacc
     5701 aaatatagta aaatgctgaa agatg
//
LOCUS       DACEOE010000153         5636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEOE010000153 DACEOE010000000
VERSION     DACEOE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.156"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            273..1139
                     /locus_tag="IW774_002272"
     CDS             273..1139
                     /locus_tag="IW774_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1154601.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(1161..1598)
                     /gene="ndk"
                     /locus_tag="IW774_002273"
     CDS             complement(1161..1598)
                     /gene="ndk"
                     /locus_tag="IW774_002273"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1154602.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            2257..4935
                     /gene="mgtA"
                     /locus_tag="IW774_002274"
     CDS             2257..4935
                     /gene="mgtA"
                     /locus_tag="IW774_002274"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1154603.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            5022..>5636
                     /locus_tag="IW774_002275"
     CDS             5022..>5636
                     /locus_tag="IW774_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1154604.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLA"
BASE COUNT         1854 a          956 c         1206 g         1620 t
ORIGIN      
        1 agcaaaaagg cgaacattcg acaagtgtct tttcaaaaaa tggtaataaa acaacaacca
       61 acttatatcc agtaacaaga acagacgttg aacagcaaaa aaataatcaa taaacaatca
      121 aaccaacctt cagtagttag acaacattct aatgcttgaa ggttggtatt ttttatgaaa
      181 aaagcatttt tttatttttt tcgtgtattt ttccttttta acgtatctat ttattgaatg
      241 tggaaaaatt taaattaaaa aggagaacta ttatgtatga tggtgaattg atcaagaaac
      301 ttcgagtcaa caagaagttg acacagtccc aattagcgga aggaatctgt tttaagactt
      361 ctctagtcgg gattgagaca aattcagtga aaaaaatgtc atttgtaaca ttgagatcat
      421 ttttagagcg aatgaacatg acacttgcag aatatgaatg gatgcgaaac cagctggatg
      481 aaccaataaa gttaaagaaa aaaagacgaa tgctagatcg ggtacaagaa gacagctttg
      541 atccttataa agagatcagc aataaccgta ggcattttaa gaaaacagaa gatctgtatt
      601 atttggtttt gaatcttcac atggtcgaaa aaacagaagt atctgagaca gaatttgaat
      661 ttttacgtta tgaatgccga atcattgaag aatacttgat gcagatacca gactatggtc
      721 aatttgaact atctattcta gctgattttc cttggatatt cagtgatcgg ttcattgaga
      781 acaattattt gaaaatcaaa aaaagaatgc gacgattagc acagttgaac aatcatgagc
      841 agtatctctt tactttttta atgaacctga cttcgtttta tatagaaaat ggtcggttga
      901 aaaaggcacg tagtgtgaat gaagatatga aacggagctt agcacataaa gaaagaagta
      961 cagtgatcta tgaaacattg atggcagatt attatcaaag attgatttct gctgctttag
     1021 gggaagaaaa gacgaaagac taccaagaat tcttcacagt gctcgaatat atgttaggaa
     1081 agaatcaacg gaggtcctta gagaaaaaac tgctggccat tgctgaaccc agcaactaga
     1141 ccagcagttt agaatttgta tcacttcgcc gctttttcag acggtttgtt tatatcgaat
     1201 ggcgggaaaa agcgggcgat ctcttttacc gcagcatcac gggaatcaga agcgtgaatg
     1261 atgttttcag tccccggcag cgcgtagtct cctcggatcg tacctggaac agcatcggca
     1321 gcttttgttg ctccaaccat cttacggacg atatcgatca cttcctcgcc gaccagcaca
     1381 agataaacga caggaccgga agtcatgtaa tcgatcagtt catcaaaaaa actgcgctca
     1441 gtcaagtgct gataatgttc ttttgctaat tcaggagtca tagtttcaaa cttcatttca
     1501 gcaatggcca gtccctttgc ttcaaaacgt tggatgatgc ttccgactaa atggcgtctc
     1561 acgccgtctg gcttgataat aacaagtgtt ctttccataa cagcacctcg tttcctacta
     1621 tatttctatt atagcgggag gtggaaaatg agacaaataa aaccgcgtga gacaggaata
     1681 taacgtgtgt attttaaaaa aagtatgaaa aacatggaaa attgatttga catcctcttt
     1741 tttagttgtt atattaacga aggaaaaccg aataagattt gttaggtgag gctcctatac
     1801 aaacataggc tactgcccaa aaacgtcgag agacgccaat gggtagaaca ggaattgtcg
     1861 agataaggct tttcataagg tagctaaaag caggtatctg ctttttacgt tgtacagtgt
     1921 caaaactcaa cgatgagatc tgagaagctt tttttaatat gctttttcaa acctttcgct
     1981 gagtatttgg cgagaggttt ttttgttgcc gcaaatcccc atcggtgaaa aataaaaagg
     2041 aggtggtcta attggaaaaa taactattcg aaaaatgatt ggaagtcatg aattaaaatt
     2101 acttagtaaa ggagatgaga acaaatggat gacagtcatt cgaaaaacaa ggatctagag
     2161 gaaaggccag ggctattatc tatctgttct cagccccttt cagttttttc tctatcgatc
     2221 cgatgaatag ctaaagacca aaagcagcga gaacagctgt cttttggaca caaaggagag
     2281 attgagatgg aaaaaaagaa agatacacaa aaaatctatg cagcttttgc tggaaaaacg
     2341 aaacaagaat tatttgaact attccatgca tcagcaaatg gattgacaga agaacaaatc
     2401 gaacgttcaa gagaaaatta cggtgaaaat gcgatttcat atggcaaaaa aacaccattc
     2461 attgtagaaa tattgaaagc atacatcacg ccatttacat tagtgttgat cgcattagga
     2521 attatttcat tcatcacgga atatgtatta gcagctcctg gagataaaga tcttttcggt
     2581 gtagccatta tcttcactat ggttatcgtc agcgggacaa tgacgttgat tcagtccgta
     2641 cgttccaatc aagcggctga aaaactgaaa tcacttgtga aagtaacggc agctgtaaaa
     2701 cgtaatggaa agtacacaga gattcctatg gaagaaatcg tctgtggaga tcttgttcgg
     2761 ctttcagcag gggatatgat accagcggat atgcgtttat tgtccgcaaa ggatttattc
     2821 atttcccaag cagcattgac aggggaaagc tatccagtag aaaagcatgc gaaagtatgt
     2881 gaagacgatc agcaaagcga aacaagttac gaaaatcttg tctttatggg aagtaatgtc
     2941 atcagtggaa gtgcggaagg aatcattgtt tctgttggaa atgaaacact atttggtcat
     3001 gtagcagaaa cgctatctga aaaaccaatt caatcaagct ttgaattagg catccataaa
     3061 acatccatgc tactgatcaa atttatgtca ttgatggctc cagcagttat cgtcatcaat
     3121 gggcttacaa aaggggattg gctagaagct tttctttttg gactatctgt agcagttggt
     3181 ctaacacctg aaatgcttcc aatgatcgtt acgacgaatt tggtcaaagg agcaagtgtg
     3241 atggccaaaa aaggaacagt gatcaaaaat ttgaatgcca tccagaactt tggtgcaatc
     3301 gatgtgttat gtacggataa aacggggaca ttgacacaag ataaaatcat tcttgaatat
     3361 catatggatt gtgaaggaaa tgaagatgat cgtgttttgc gccatgcgta tctaaatagc
     3421 tactatcaaa cagggctgaa aaatttgtta gatgtggcta tcattgatga agcaacagaa
     3481 actttagata cagataagat caattatcaa aaagtggatg aaattccttt tgattttgaa
     3541 cgtcgtcgta tgagtgttgt ggttgctgat ccagctggta aaacacaaat gatcacaaaa
     3601 ggcgcgattg aagaaatgct gaacatttct aaatttattg atcttggcgg tactgttaca
     3661 ccattaacaa aagaacgaaa agaagccgta cttgcaaaag tacagtcact taatgaagat
     3721 ggcttacgag tgattggtgt cgcacaaaag accaatccaa gcgtcgttgg agaattttca
     3781 gtcaaagatg agtcagacat ggttttgatt ggttatcttg ctttccttga tccaccaaaa
     3841 gaaacaacga aacaagcgtt gaaagctctg aaagaacatg gtgtagctgt aaaagttctg
     3901 actggggaca acgaattagt tacacgttca gtgtgccgtc aagtaggtct agagatcaat
     3961 gaattggtta ccggtgagaa aatctcggaa atgagtgatc atgaattagc acaagtagct
     4021 gaagaccatg aagtattcgt aaaattgaat cctcagcaaa aagcacgttt gacgacagct
     4081 ttacgtaaaa atggtcatac tgtcggattt ttaggagatg gtatcaatga cgctcctgca
     4141 atgaaagtgg ctgatatcgg tatttctgta gatacagctg tggatattgc gaaagaatct
     4201 gcagacgtga tccttttaga aaaagatttg acgattttgg aaagaggcct tctttctgga
     4261 cgagaaactt ttgggaatat catgaaatat atcaaggcta cagcaagttc taattttggc
     4321 aatatgtttt ccgtgctgat tgcaagtacg ttcctgccat ttttaccaat gctgccttta
     4381 caacttttgt ttttgaatct gatctatgat gtttcctgca tttccttgcc atgggatcgg
     4441 atggataaag aatatttgga cgagccaaaa aaatggaatg catctagtat tggggaattc
     4501 atggtttact ttggtccaac aagttcgata tttgatatta ctacttactt gttgatgtat
     4561 tttgttattt gtcctgctgt agtgggcgga agtttccata cattggatag ttcacagcga
     4621 gtgattttca tcgcattatt ccatgcagga tggtttgtcg aatcactatg gacacaaacc
     4681 ttagtgctcc atgcactgcg tacaccgaaa gtaccattta tccaaagcaa cgcaacattt
     4741 gccatgtttg ttatcactac attaggaatt attgtcggtt ctgtccttcc ttttacaagt
     4801 tttggtgctg aattaggctt gatgccactt cctggcaatt actggttatg gttatttgct
     4861 acagtcatcg cttatctggc gcttgtgaca gctgtgaaga aaatctatat ccgtcgattc
     4921 caagaactct tataaacgag aaggctggtt ctttgcttct gttctttttg gaccagtcgt
     4981 gctaaactat gtgtattgtt ttggaaagga ttaatgtata gatgaaaggt gcaatatttg
     5041 atttagatgg tttgttagtt gacacagaaa aaacgtatcg tgatggctgg ctttggggat
     5101 ttcagcaatt tgatatccat atcccaaaag aaatcgttga cgcttggggc ggaaaaaact
     5161 ggaaacaaag ttttgatatt ttagtgaaag cagcaggtag ccctgaaaaa gtacaggaag
     5221 tccgagcgaa gcgtgaagaa tatttttatc aacagctgac aagcggagcc atccacttaa
     5281 aaccatatgc aaaagaaata ttggcagact taaaagaacg tcatatgaaa ctaggcattg
     5341 ctacgaccac tgttacgaaa agagcaacag atatcttgga tcactttaaa ttagctgatt
     5401 acttggacac gtttacattc ggagacgaag tgtcggagaa caagccttca ccgattcctt
     5461 atctgacagc tttggaacga acaggattag aagctagttc agcctttgca gtggaagatt
     5521 ctttagtagg agcaacagct gcttcacgag caggattagg cgttgttttg atacctgatt
     5581 ccagttttgt ccgaaattat accgtcgaag aaaaggaaaa attgaaactt cttgcg
//
LOCUS       DACEOE010000154         5525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEOE010000154 DACEOE010000000
VERSION     DACEOE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.157"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(67..537)
                     /locus_tag="IW774_002276"
     CDS             complement(67..537)
                     /locus_tag="IW774_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1154605.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            867..2507
                     /locus_tag="IW774_002277"
     CDS             867..2507
                     /locus_tag="IW774_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1154606.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2661..3983
                     /locus_tag="IW774_002278"
     CDS             2661..3983
                     /locus_tag="IW774_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1154607.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4051..4485)
                     /locus_tag="IW774_002279"
     CDS             complement(4051..4485)
                     /locus_tag="IW774_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1154608.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4550..5386)
                     /locus_tag="IW774_002280"
     CDS             complement(4550..5386)
                     /locus_tag="IW774_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1154609.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1650 a         1099 c         1009 g         1767 t
ORIGIN      
        1 tttttcacga cctctgattc acggtgtttc tttttcatca acatttttac gaaatactgt
       61 ggaagactat tgaaataagt gtggattgtt tctttgtgtt tcaatcgttt cttgccattc
      121 ttcatttaat tttggattac cgttttcttc cttgaactca taaggtccta attcaaaaat
      181 cccagacagt atttgattca cgatttcata tagttcagtg tgatcagaca catgattctt
      241 gaagatcatc tcattatcac cacgtttgaa cctcacattc gggctctcat gatgaacctc
      301 tatgcatttt tctccttttt ttaactgacg gacttctgaa aacatctcat aaatatcttc
      361 tccagtaaaa ccttcagcaa gcgccagact gtccacaaga ccgcctactg tcagttcaga
      421 gattattgct tgattggaat ctaattttaa tgtatagctt gtcgcttcta ctacgccaca
      481 tgcattgcaa ccgccttctg atagttcgct ttttctaatc aattgaactt ctttcatttt
      541 ctcttcctcc tcttaatttt ctacttatgt atttttatca cctgacatag gtgactccta
      601 tcaacgctat catgtgcttt tttcctcaga aatcctattt atccattcca tctatccctt
      661 attcttctac ttttctttct ctagcatata agagatacgc atagtattca agcaaggatt
      721 gtcgatataa aaagaacaaa tactgctatt ttgatttctt ttggctatat tacatataaa
      781 taaaatttat catacctgct ctttttgact atatcatcat ctgacttcta tacttcgttt
      841 gtattaaaac ttaggaggaa tcattcatgc ttacagggtt aaacttatta aataatcctt
      901 ttctaaataa aggaacagct tttacaaaag aagaacgggc aaaatatgga atcactggta
      961 tgcttccaag tactgtccaa acactggaac aacaatctgt tcaagcttat ggtcaatact
     1021 taagcaaaca aacagatttg gaaaaacgta tcttcttaat gaatctattc aatacgaatc
     1081 ggacattgtt ttataaattg atgggcgaac atttagttga attcatgcct gtcgtttatg
     1141 atcctgtcgt tgcggattca atcgaacaat acaacgaaat tttcttgaaa ccgcaagatg
     1201 ctgcatttct ttcaatcgac gaaccagaaa acatcaaagc aagtttgaag aacgcggcag
     1261 atggacgaga cattcgtttg attgttgtga ctgatgcaga aggaatctta ggtatgggcg
     1321 actggggtgt gaacggtgtt gacatcgcta tcggcaaatt gatggtctat actgctgctg
     1381 ctggtattaa tccagcacaa gttttgcctg tatcgatcga cgctggtact aataatgaag
     1441 aattattgaa taatccctta tacttaggca atcgtcatgc tcgtgtagaa ggcgaaactt
     1501 actatgaatt catcgatcaa ttcgttcaat cagctaccga attattccca gaattgttat
     1561 tgcattggga agattttgga cgaggaaatg ctgcagcgat cttagaaaaa tacgaagatc
     1621 aaatcactac tttcaacgac gatatccaag gtacaggaat tgttgtccta gcaggtgtat
     1681 taggcggatt gaatatatcc ggtgaacaat tgaaagatca aataattttg acatttggtg
     1741 ctggtactgc tggtgtgggt atcgcaaaca tccttttaga tgagatgatc cgtcaaggtg
     1801 tacctgaaga agaagcacgc caacatttct atcaagtaga taaacaaggt ttattgtttg
     1861 aagatacaga aggattgact cctggtcaaa tcccattcgc tcgcaaacgc tcagatttca
     1921 ctaatgctga tgaattgaca aacttagaag cagttgtaaa agcgatccat ccgacaatca
     1981 tgattggtac ttctacccaa cctggagcat tcactgaaga aatcgtgaaa gaaatggctg
     2041 ctcatacacc acgtccgatc atcatgcctt tgtctaatcc aacaaaattg gcagaagcaa
     2101 aagcaaaaga tttaatagaa tggacggacg gaaaagcatt agttggaaca ggtatccctg
     2161 ctgctgatgt tgaatataat ggtgtaactt accaaatcgg tcaagcaaat aatgctttga
     2221 tgtacccagg tttaggactt ggtttgattg cttcaacagc tacacgagta aatgctgaaa
     2281 tcatttctca agctagtcga gcattaggcg gaatcgttga tgtaacaaaa ccaggagcag
     2341 ctatcttgcc tccagtagca aaaatcactg aattttctca aacgattgct gaaactgtcg
     2401 caaaatcagt tgtcgcacaa aacttgaatc gtgaagaaat cacggatatc aaagaagcag
     2461 ttgaatctgc taaatgggtt cctgaatata aatctttaga agattaagat cgttatagta
     2521 aaagaacata cagaaacttt gtatgacgtc taaaaagaaa aaagcgactg cggcaaaaca
     2581 gcggccagcc aactcctgct cttttgtcgc ttttctttgg cttttttcat atgatacaga
     2641 ataagaaagg atctgtaaaa atggcacaaa aaacaacaac tgcaactgca gttgaggaaa
     2701 agaaaaaaga aggtttttgg tccacaaaaa tcaccggcgt aagtttgcca ctttatttga
     2761 tcatggtcct tgtcctgatc attgcaatgg ctttagggaa attaccgacg aatatgattg
     2821 gaccaatcgc agttttggtt attttcggta atttatttca ttttatcggg acaaaaatcc
     2881 ctatcgtcaa aagttatttg ggtggtggtt ccgtttttgc gatcttcgct tctgctgcga
     2941 ttgctacatt tgggatctta cctgaatatg tagtgaaatc cacagaaaac tttgtcagca
     3001 atatgggctt ttgggacttt tatattgctg ccttgattac tggtagcatc cttggtatga
     3061 accgtaatct attgatgaaa gcttctgttc gttttattcc cgttgcactt atttcaatgg
     3121 tcgtctcctt ttttgccgta ggactcgtgg gcatgcttat cggaaatggt tttgcaaatt
     3181 ccgttttata catctcatta cctgcgatgg ctggtggtgt tggagccgga gccgttcctt
     3241 tatcaacgat ttatgcaaat gttttaggta cggatgcttc ttctatcatt tcccgcctga
     3301 ttcctgcgac agcaatgaca aatgtcttag ctatcatcgg tgcagcatta gttgctcgtg
     3361 ctggacaatc aatgccaaaa ttcaacggca atggtaaatt aatggaaaaa ggcgatcttg
     3421 gcgatggaaa acctcgaact gtgaaacctt ccattccaca gttaggtgtt ggtttgatgg
     3481 tatcattgac tttcttcatc ttaggacaaa ttggtaacta ttttgttcct aaagtccatg
     3541 cttacgcatt tatgattatt atcgttgtca tctgtaaaat cgccaaccta ttaccagagt
     3601 attatgaaga tgcagctatc atgttcaata acttgattgt caaaaacttg acagcagctg
     3661 ttcttgccgg tatcggtatt gcgttgctga atctgaatgt attagcttct gctttgacat
     3721 ggcaatttgt tgtcttatgt ctgacaagtg ttatcgcgat ttcagttgtt tcaggatttg
     3781 tcggtcgttt gtttggttta tatccaattg aatcaactat tactgctggc ttatgcaaca
     3841 actcgatggg cggaacagga aatgtagccg tcttatctgc agctaatcgg atggaactta
     3901 ttgcttttgc tcaaatgggc aatcgacttg gtggtgccat cgttttgatc atctctggtt
     3961 tcttgatgca attactttct tagtcaaaaa aacaaaaata cctgatcatc aagtatcggc
     4021 ttggtgatcg ggtatttttt tgataaagat ttacaggatt ttcaaaaatc ttttttgcat
     4081 tttatcggaa tgataaccga tgtgttcata aaattggtgt gcttctactc tgtattctgc
     4141 agaattaagc cttattgtcg ttattcctcg ttttttcgct tcttcttcta cgccagtcat
     4201 caaagccttt ccgatgcctc ttttttcagc atatttagac acagcaagtg ccataatatt
     4261 gaacattgga tcgctaaaaa tcgattcata ggactccgct tgtacatagc cagctgcttt
     4321 tttagaagta ggatcttcat aaaccaagaa aaaatttttc tctggctcct tcagcagttt
     4381 cttcatttgt ctcaccgtaa cggttaatgg tatatcatac cctaattgat ccgcattgat
     4441 ttcacgtagt gcttctgcat cttctaaacg catttcccgt aacatccaaa tcaccagcca
     4501 atttctgatt ttttaaaatg aattttttta tcatagtata ctcttttttt catttagaac
     4561 aatatgtttc agtgattttg acttgcgtta gtctagattc tcctaatgtt tgtaagatga
     4621 caaaggcatt gtaacaattg accaaaagaa ccacgaggac aattacttga aaaataaagg
     4681 tcactttttt tggagacaaa accctgctaa atcttgctcc ccacaaacct ccaataacag
     4741 cagcggggat aacataaaac aacatagata aatcaaagcg cataaagcta cttgttaaag
     4801 caatcgttac caattttgcc agttgagaaa agaaaatcgt agatagagaa tatagtgttg
     4861 cttcttttat tggcaatgca aacatcagca tcaacaaaga aacattgact ggtccaccac
     4921 caatacctaa aaaactagct aaaaagccca gaaccccccc acatataagg taccaagtcc
     4981 atgacgtcaa atgaaatttt ggctgatgat gcttggtgta gaagaacgca aaaaccaagg
     5041 tcaataccgt caaaaaaatt tggatcagct gcacatgctt ctcgttttca aataactgta
     5101 aaaaaacttc gaatacaatg ttgccgacta ctcctccaac aacagctcca ctagataccc
     5161 aaccgacaat ctgccaattc agtgatttcc cgcttgatac ttgtcgcaca gtcgaaacaa
     5221 tggacatcgt gaataccgca actgttgagt aaaaagaaat ccctgcaaca gaatgggcgc
     5281 cgataagatc aagaatcggt ttgatcaaca cgcctccacc catcccggaa atagcgccaa
     5341 ctgtgttcgc cagaacgatc accacaaaat aaatcagacc aatcatcttg tatttccttt
     5401 ctcttgttta tatcttttag cgattgttac tcttttcttt tattatacag taaaagaaac
     5461 aaacgaggct tcgaaagctt ttctactttt taaggaaatt tagtgctaaa cgaaaagagg
     5521 ttgtg
//
LOCUS       DACEOE010000155         5517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEOE010000155 DACEOE010000000
VERSION     DACEOE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.158"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(24..977)
                     /gene="menA"
                     /locus_tag="IW774_002281"
     CDS             complement(24..977)
                     /gene="menA"
                     /locus_tag="IW774_002281"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1154610.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(990..2093)
                     /locus_tag="IW774_002282"
     CDS             complement(990..2093)
                     /locus_tag="IW774_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1154611.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2183..3118)
                     /locus_tag="IW774_002283"
     CDS             complement(2183..3118)
                     /locus_tag="IW774_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1154612.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3420..5354
                     /locus_tag="IW774_002284"
     CDS             3420..5354
                     /locus_tag="IW774_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1154613.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT         1604 a         1085 c         1098 g         1730 t
ORIGIN      
        1 tttttcacaa cctcttcatg ctgttaaatg gcttgcataa tgattgttag taatagcccc
       61 aatacataac tggcattgaa taagaccata tttttgatgg aatagctgaa actgataggc
      121 tggggtaacg tttgcttgaa ttgatccaaa tgcttcttca caattggcaa ggtcaagaag
      181 gtgaccaaga tcggccattc atagataccg ctcaacaacc ccattaaaat cacgaaatag
      241 caagcataca tcaatagctg gaataagact gttcccgcag gacgtccgat ataaaaaact
      301 agtgtatagc gatgattttc aatatcacgg tcaagatccc gcaaattatt agccaacatg
      361 atattggcaa tcgtaaacac caagggtaag gaagctaata cgattgctgc tatagcccaa
      421 agatttccta ccaaatcaaa ttgtccagat gaaaagttga ctgtcagatc atagacatct
      481 gaaccgaatg tattgacata aacgaccatg gcaaaaattc caaggcccat agtcacccca
      541 ctgaaaattt cccctaaagg cattcgagat aaaggaatcg gtccgaatgt atagaatact
      601 ccaataaaac aacagataaa tcccatcaaa agtaaaagcc aacccgtatt gatcgttaga
      661 ataacgccga taacaaaaac aaatgcaatc atagctaaaa tcatgtttcg aatcaataca
      721 ggagatagat ttctgcggcc aatgacattt tcttcatact tatatacttc agaatgagcc
      781 ttttcataat ccatgtaatt attgatagcg gtagtcgcta aatcaaaaac tagcattcca
      841 ataaaaaata aaaaagtgtt tagccaatgt atctcatgaa aaaaagcgat agagaataat
      901 acccctaccg caaaaggaaa tacactagcc acctttgttc gtatttcaac tacttctaaa
      961 aagattttta gtgtcatggt tcgtactcct tattttagtt cagaacggtc acccatcgtg
     1021 taatgggagt cctccgataa tttgaaggtg tctttgattg gatcggtcac atatacttta
     1081 tcgtcttttg tcacgaagat cgcttccgtc ccgttattca attcgctttc gacgtattcc
     1141 agtccttttt tcacacccat tgaaaagact gctgtagaca acccgtcacc atcaattgat
     1201 ttatctgtta tgatcgtgac acttgcgata tcattgtcga acggataacc cgtttttgga
     1261 tcaaacaaat gatggtattt tttgccatct acttctaaat aacgctcgta aattccagag
     1321 gtaactaatg ttttgttccg ttctttaata gaacctaaaa cagatccgcg agccatgttc
     1381 ggatcttgga tgccgactgt ccagtcttgg ttttcgcctc ttgggctgtg gcctaagaca
     1441 tagacattac cgcccaagtc aacgatggca gtggtcactc cttgtttttt caagactttc
     1501 actacttcgt ccgtaatgta acctttggcg attgcaccta aatcgatgat cattcctttt
     1561 tcttttagat aaacagtttt ttctttgtca tttagttcga tcttatgata atccactaat
     1621 ttcaatgcct gatcgatttc ttcttgggaa ggttttctgg cgtcatcaaa accgattcgc
     1681 cataattgtg tgattgcacc aatcgccata tcgaatccgc cttgagaatc ttggctatat
     1741 tcgtaggccc gcttgactaa cgtgtagacg tcatcagaaa cttttactgg tttgatccca
     1801 gcttgctcat tcacttcatc gatctcagaa cctttttgat tgatcgtgat tttatcacca
     1861 agttctttta ctcgatcgaa ggcaggtttc aatgctgatt ctttgccttc atcatacaca
     1921 cggatacgaa cgtaagtccc taacaagaac tgttcatcag aatatggatc tttcaatatc
     1981 ttggtagctg ctgaactttc agtggttgtt ttatttgctg agttgctgca gccagttagc
     2041 accataaaag agatgcccag tgtgattaag gaaaatagta gttttttccg catctttttc
     2101 ctctcttcat tttgaaaggt gagacgactg cctaatctgg ctatcgtccc accctccaac
     2161 ttttattagt atgattatgt gattattttt ctgttacgat attatctact tcgattgttt
     2221 gagtgtcgcc tttttcagct gcattcacta attgttgtgc gtacatgatg aatgaatgtg
     2281 agctgtgtgt agcacctgta acaacttcta cattagcagg tgagccatct tcttcgccca
     2341 ttgctttgac gaattcgtcg ttcagagtag ggatgtaagt ttttggttct gtaccgcttt
     2401 ttgctttcat attttcatta tattctgtat catcttgttt tgatttaccg tctttgtcga
     2461 tataatcaaa gtttgattca gttactttgc cgccagcaac tgtcatttcg aattgcacac
     2521 ggtagccgtt agagtagttc ttttcttcaa gcttgtaagt gccgtctttc aaatcagcac
     2581 cattgtcgat ttcgattgta tctgtgttac cagcttgagc agcttggatc aattgttgtg
     2641 cgtagttttg gaagctttca gatgagtgag tagctccagt tacgacttcc acaccgcttg
     2701 cgctttgtgc ttttaagaat tggtcattca actcaggaat gtatttttct gggtttgtgc
     2761 cagattttgc ttccatgttt ttgttgtatt ctgcatcttt tgtttttgat tcgccgtttt
     2821 cattgacgtt atcatatttt gattcagtga ttttgccgtc ttttacaacc atttcaaaga
     2881 ctgcacggta accatttgaa taattttttt cttctaattt atatgtgccg tcttgcaaat
     2941 ctccgccagc tacaactttt gcagatgatt ctgttgtgct ttttgcagtc gaagattgtg
     3001 ctgtagatga ttcagaagaa gagtttgagc ttccgttgtc atctgcgccg caagcgccta
     3061 acactagagc tgaaaatgct aatacggcaa atccagttac aaaacgtttt ttcttcattt
     3121 taatacccca ccattccatt tctagtccac aatttttctt tgtgattcat tatacattct
     3181 aatcttttta gcctataagt caactatttt tgataaataa cccatgtaat atttcaaacg
     3241 ttttctttca gcaaatttcg tgaaaaaacg ctttatttta aaatttgaaa aaaatccaaa
     3301 aggagttaga caaaagaaca gaacatctat ataatatatg gagattgtgt aataagtatc
     3361 aaactaaaag aattatcata tattcattat atagatagtc tataaggagc ggatgatcaa
     3421 tggcaaagca aaatattgtc gttgtaggag caggatatgc cggtgtttca gctacaaaat
     3481 acttggcaaa gaaactgaaa aaagaagatg tagcaattac tctgatcgat cgtcattcgt
     3541 accatactat gatgaccgag ttgcatgaag tagctggcgg acgtgtagaa ccagaagcaa
     3601 tccaatacga tctgcagcat ttgtttgctc gtcaaaagaa tgtacaactt gtcacagaca
     3661 ctgtaatcgg tatcgataaa gaacgaaaaa tcgtcaagac caaattaggt tcctatcctt
     3721 ttgatcagct gatccttgga atgggcggag agccgaatga ctttggtaca cctggtgtca
     3781 aagcacacgg attcaccctt tggtcatttg aagattctgt aaggattcgg gaacatattg
     3841 agaagacagt agcaaaagct gcattggaac cagatgatga actgcgtaaa gccatgctga
     3901 ctttcgttgt ctgtggttct ggatttaccg gtattgaaat gattggggaa ctgatcgact
     3961 ggaaaaatcg cctagccaaa gattataaat tagatccaaa cgaaatcaca ctgatggtcg
     4021 tagaggcaat gccgacgatc ttgaatatgt tggatcgaaa tgatgctgca aaagctgaac
     4081 gctatttgcg taagcacaac gtggaattat tattgaatgc accaatcgta gaagtggctg
     4141 ctgatcatat caaattgaaa gacggacgca acattccgac tcacacactg atttggacag
     4201 ctggtgtcaa aggaacttct gacgctgctg actttggatt agaatcagca cgcggacagc
     4261 ggttgatcgc caatgaatat atgcaggcaa aaggctacga agataaaaac atctatatca
     4321 ttggtgattt agtcttttat gaagaattcc caggaacacc gacaccgcag atcgttcaag
     4381 ctgctgaaca aacaggacac acagccgcag cgaatatcgt ggcttcgatc aaaggcggag
     4441 aaaagcataa atttaaagga aactatcaag gatttatggt ttctattggt gccaaatggg
     4501 gggtagctaa cctatttgat aagatccatt taagcggatt tttagctatc gtaatgaaac
     4561 atatcgtgaa tttgaaatac ttctttgata ttcgttctgg ttattatatg ttccaatatt
     4621 taatgcatga gtttttccat atcaaagatg atcgcaacgt gactcgagga cattcttctc
     4681 gttacggcaa tgtcttatgg agtgtgccac ttcgtatttt ctatggttta gtttggttcg
     4741 ttgaagcttc caaaaaaatc attgggaatg gtgaagtgtt caaaccaagt acttggtttg
     4801 gtgaaggatc atggttcact ggaaacagtg tgttcccttg gacatgggaa tacaatgtaa
     4861 cagatgttgc cagtggtgct tctgctgctg gtgatgcaac cacagctgcc agcggagcag
     4921 gggcaagtgc cggagaagca gcgactcaag ctgcacattt tggtttgagt tatgcatacg
     4981 gtgaagaacc aatggctgtg attgagaagt caccagactg gttcacaaag atcatgcaag
     5041 tgatcatgcc aaacaatgat gtagcaatgt tcttccaaaa attcatggta ttctttgaaa
     5101 ttgccttggc attagcattg attgctggat tattcacatg gctatgcagt gcgacaacga
     5161 ttgtattggt gatcacgttc tgcttatcag gcatgtttta ctgggtaaac atttggttca
     5221 tttttgttgc tttcgcattg atgaacggtt ccggacgtgc gattgggttg gatcgctggg
     5281 taattccatg gctacaacgt acactaggta attggtggta cggtagacca agttcccagt
     5341 acggcaagaa ataaatagat aaagaacgtt atttgaatgc aagatttcaa tagaaagggg
     5401 ctgagacaaa ccgtcacagc ctccttttta tgtaaaatta aaggaataag atgcccgaga
     5461 taagcctcca tgagcaagac acaggtgtca caacctctgt tacaacctct tgttata
//
LOCUS       DACEOE010000156         5410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEOE010000156 DACEOE010000000
VERSION     DACEOE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.159"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(57..350)
                     /locus_tag="IW774_002285"
     CDS             complement(57..350)
                     /locus_tag="IW774_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1154614.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="IW774_002286"
     CDS             complement(378..1043)
                     /locus_tag="IW774_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1154615.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="IW774_002287"
     CDS             complement(1183..1815)
                     /locus_tag="IW774_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1154616.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="IW774_002288"
     CDS             complement(1822..2889)
                     /locus_tag="IW774_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1154617.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="IW774_002289"
     CDS             complement(2886..3617)
                     /locus_tag="IW774_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1154618.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IW774_002290"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IW774_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1154619.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..>5410)
                     /gene="mltG"
                     /locus_tag="IW774_002291"
     CDS             complement(4401..>5410)
                     /gene="mltG"
                     /locus_tag="IW774_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1154620.1"
                     /translation="GFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDN
                     IIKSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAI
                     PEGYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRL
                     EGYLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEK
                     EGVKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTN
                     TGYGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNN
                     S"
BASE COUNT         1545 a         1148 c          829 g         1888 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa
//
LOCUS       DACEOE010000157         5395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEOE010000157 DACEOE010000000
VERSION     DACEOE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.160"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(158..616)
                     /locus_tag="IW774_002292"
     CDS             complement(158..616)
                     /locus_tag="IW774_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1154621.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(629..1954)
                     /locus_tag="IW774_002293"
     CDS             complement(629..1954)
                     /locus_tag="IW774_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1154622.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGRDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1971..3383)
                     /locus_tag="IW774_002294"
     CDS             complement(1971..3383)
                     /locus_tag="IW774_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1154623.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3400..4164)
                     /locus_tag="IW774_002295"
     CDS             complement(3400..4164)
                     /locus_tag="IW774_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1154624.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4268..5143)
                     /locus_tag="IW774_002296"
     CDS             complement(4268..5143)
                     /locus_tag="IW774_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1154625.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1666 a         1122 c          780 g         1827 t
ORIGIN      
        1 cctgaaaata agttttctaa atcataggag caacaaaaaa attcgataat taacaaaaat
       61 caaaatgatt aaatctagtg attgaaacat atcatttcaa aaaaattttg taaattcacc
      121 cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa caactatctt
      181 taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta
      241 caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca
      301 gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga
      361 gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg
      421 ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat
      481 atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt
      541 cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg
      601 ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta
      661 ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca
      721 atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact
      781 tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta
      841 gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg
      901 accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat
      961 cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt
     1021 tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct
     1081 ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca
     1141 tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg
     1201 tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata ttaaaatcgg
     1261 cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt tctcgttgca
     1321 attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa tacgtaacga
     1381 tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca
     1441 tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct tcagaaacga
     1501 tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct tgtatatgat
     1561 cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct ccgagtccac
     1621 acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct
     1681 ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgcg tcctgtaaaa gcaagctttg
     1741 gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc ataaatttaa
     1801 caatcaagta catatccata tttctttctt catcaatatc atataaacgt atttcagata
     1861 atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc
     1921 cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt ctattcttct
     1981 aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat aatttgataa
     2041 tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg ctgttcctcg
     2101 accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata gacatccagg
     2161 atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc ataattttta
     2221 cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc tgccttagct
     2281 tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt aacgtaatac
     2341 aaggcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc gattcgaaaa
     2401 ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat attgaataag
     2461 taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa cataggagaa
     2521 agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga agttcctgca
     2581 ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc tttatagata
     2641 gctagacata tagctggtat accaaacatc attactacaa aaaaaactga taaaaacagt
     2701 ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac ttccttgcta
     2761 ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg gtgcagtcca
     2821 aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc tcccatatca
     2881 acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat aaagcccaaa
     2941 aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt ttcgcccgca
     3001 aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt ccatgccgct
     3061 actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc cataatttct
     3121 aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc
     3181 ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa gtttccaaaa
     3241 acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc tgataaacgc
     3301 atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct tccaagcttc
     3361 tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct catatgttac
     3421 taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc
     3481 gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct gatccaaata
     3541 cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga gtaaacttgc
     3601 caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt ctcctacgac
     3661 atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt caaagttgct
     3721 ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat tttcatccaa
     3781 agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat gataaatttt
     3841 ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa atggatgctg
     3901 ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag ttttgaggag
     3961 tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca tttcctttct
     4021 tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg atgtcaaaat
     4081 tcctcgctga taagcatcca taatcccata attcacacca tggctatagc caaaatcatc
     4141 cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa aataggcgca
     4201 accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc
     4261 ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa
     4321 tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt
     4381 tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat
     4441 aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt
     4501 ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt
     4561 ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca
     4621 tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct
     4681 cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca
     4741 taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg
     4801 tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa
     4861 tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc
     4921 gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg
     4981 gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat
     5041 cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc gttaattcac
     5101 ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca
     5161 aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat
     5221 tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa aagcgttatc
     5281 aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg aggtaattac
     5341 ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg ataat
//
LOCUS       DACEOE010000158         5358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEOE010000158 DACEOE010000000
VERSION     DACEOE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.161"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2185)
                     /gene="nrdD"
                     /locus_tag="IW774_002297"
     CDS             complement(<1..2185)
                     /gene="nrdD"
                     /locus_tag="IW774_002297"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1154626.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHM"
     gene            2511..3278
                     /locus_tag="IW774_002298"
     CDS             2511..3278
                     /locus_tag="IW774_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154627.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            3268..5310
                     /locus_tag="IW774_002299"
     CDS             3268..5310
                     /locus_tag="IW774_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1154628.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         1591 a          940 c         1038 g         1789 t
ORIGIN      
        1 tcatgtgttt cacacgtgag gaaatttctt tatggcggcc atgtaccatc ggacgagctt
       61 gcggatttcc taaatagcca caagtacgtt tgactacatc acatgttttt ggatcatgat
      121 ttccacattc tggacattcg aaccctcgtt ctgtcggttt gaaatcacct tcgaaaccac
      181 attgatagca atgatcgatt ggcgtattcg taccaagata cccgattttg tcatatgcaa
      241 aatcccatac agcttctaaa gctttcggat tttgttggag catagggtat tcacagtagt
      301 ggatgaatcc acctgaacaa tattttggat aatccttctc aaattccaat ttttcaaatg
      361 gcgttgggtt tttacgcaca tcatagtgga aactgtttgt ataatattct ttatctgtga
      421 tattttcgat gatgccgaat ttttctgtat ccaaacggca gaaacggtca gttagacttt
      481 cacttggagt tgaatagaca ctgaaatgat aaccatattc atccccccat gcatctgcat
      541 tagcttttaa ttctttgacg atatcaagtg tgaaagcttt tgcttctggg tttgtttccc
      601 attcgccacc gaagaaagct gaagcaactt catacaatcc aatgtagcct aacgatacag
      661 tagctcgacg gtttttgaat aattcattga cagaatcggt acgtgaaaga cgtttcccga
      721 aagcaccata catatataaa attggcgcat ttgctggaat ggcttcttta caacgttcta
      781 cacgatagag aagcgcatct ttcatgattt ctaagcggtc agacagtaac tgccagaatt
      841 tattctgatc tccttttgat tcgatcgcga ttcttggcaa attcaatgta acaacaccta
      901 aattcattcg tccgacattt acttcttgac cattttcgtc tttccaccct tgcaagaatg
      961 aacgacagcc cataggtact ttgaaacttc cagttaattc aacgatttta tcgtaattca
     1021 gaatatcagg atacattctt ttcgttgcac attccaatgc tagttgtttg atgtcgtaat
     1081 taggatcttc agggtttaag ttcacacctt ttttcaaagt gaagatcagt ttagggaaga
     1141 tagctgtccg cttttcactc cctaatccat tgattcgaat ctgtaaaatt gctttttgaa
     1201 tttctctttc aaaccagttt tctcctagtc caaatcctaa tgaagtgaaa ggagtttgtc
     1261 catttgaagt aaataaggta ttgatttcat attccaaact ttgcatagca tcgaagatat
     1321 cttttttggt ttttgctttt gcatattctt tttggcgttc tggcgaatcg atccattctt
     1381 ccgcgtcttt tagatgtttt ttatagttca attctgcaaa aggagcaagc aattcatctg
     1441 ttcggtcggc agaacagcca ccatattgac ttgacgctac attggcaata atctgtgaga
     1501 tttgtgctgt ggcggtctgg atagattttg gcgattcgac ttcggcattg ccgattttga
     1561 acccattgtt cagcatgcct ttgaaatcaa tcaagcagca attcgtcatc ggtgtataag
     1621 gatgataatc caaatcatgg taatggatat cgcctttttg atgcgcattt gctatatgtg
     1681 gtggcaacat tttcagtccg attgattttc caacgatccc ggctgtcaga tctcgctgtg
     1741 tattgaaaac attactgtct ttatttgcgt tttcgttcac gacagtttgg tccttgttga
     1801 ttagcttttc aatcgaaaag ttgatatctg ttgctttact gcgagcaaaa tctcgtttgg
     1861 tacgataatt gatgtactct tctgctaatt catattcgtt tttttctagt aatatatgtt
     1921 ccacgatgtt ttgaatctca tagattttga cattttcagc aaaccggctt ttgatttctt
     1981 ggacaaccaa gtctgcaatc atttgaattt tttcataagt aagtggtgat aatgagccgt
     2041 gaattttctt ttctgctttg atcagtgctt cattgatctt ttgttcatcg aaatcaacat
     2101 ggcggccatc gcgtttgatg actttcatct gggatgtctc tggctgctga gtaacgacag
     2161 ttttttcttc ataatttact ttcattatct tcaactcctg ctaaattgtt cttgtctatc
     2221 ataaaacaaa atcggggaag gagcaactaa atatcggcta tatatagtat tcaatgtgct
     2281 gtataattgg atttctatac tatatgtagt gtatatttta ttgtgaaaaa aggtgaaaac
     2341 ttgtagctgg tggatttgtt ttttgcatat ttaattttgc tgaataaaat aattgaataa
     2401 aattttttat tcagtccaag taaaagtgac aaaaacataa gagcaaatta ttcgtagata
     2461 tgctactatg caaataaata gaaatgaaaa atgaaagagg aggagaagaa ttggaaaaga
     2521 tgatcgaagt gaaacatcta agtaaaagct acggcagcaa actttctccc gtcaaagcct
     2581 tagataatat ctcattcaca gtagaagaag gcgagtttgt tgggattatg ggcccaagtg
     2641 gtgcaggaaa gacaacgctg atgaatatct tgtccacgat caatttgcct acgatgggca
     2701 gtgtctcgat ccaaggaaaa gaaatcacaa aaatgaaaaa cagtgagctc agtgactttc
     2761 gtcgaaaaaa actaggattc atttttcaag agttcaattt gatcgatacc ctaaatgcaa
     2821 aagacaatat cctattgccc ctagcagttg aacggatgac taaagaggaa atggaaaaga
     2881 gagtaagaca tgtagctcaa ttgttgaata tagaagaact gctcaaacgg tatcccgatg
     2941 aactttctgt cggacagaga caacgaattg cagctgctag agcacttgtt gttcagccac
     3001 aagtgatttt tgcagatgaa ccaactggct ctttggactc gaagtcggct acagaattgt
     3061 tgaactactt gaagaccatg aatcaaaaag aaaaagcaac gatactgtta gtcacccatg
     3121 atccatatac agcaagctac tgcgatagaa ttttatttat taaagatggt gtgatttttt
     3181 ctgaagttgt cagaagaggg acaagaagag agttctttga gaaagtaata gacatgcagg
     3241 cgacgattgg cggaggagga aaaatgaatg ctgtataaac tagccgttaa aagttttttt
     3301 cggcagtcac gaggctatct tgtttatttt ttcagtttga ctttatccgt aatgatctat
     3361 tattccttca gcgcaatgac ctacgatcaa tctttagtca gaagagcaag tcaagatact
     3421 tcgatagatg gtggattgag ttttggtgga tttatcatta ctgttgtttt gctttttttc
     3481 gtattcagtg ccaaccgatt tttcttgaat agaaggcaaa aggaaatagg catctatcag
     3541 ctttttggta tgaataaact gcagatatca ggtatatatg tgttagaaat catgataatt
     3601 ggattgttcg cctgtatttc tgggatatta ctcggtatta ttttttccaa actcttttca
     3661 atgattttgg ttcgaatgat ggatatggat cttactagtc cattttttat ctccattcca
     3721 tcagtagtag atactttatt tgcttttttc atcatccttt tagcagtatc tgtatattca
     3781 atatggaaaa tatggcgcta tccaatgatt cgaagttttg gagaaaaaga acaagcagat
     3841 agttcaacaa tgcgcttcag gacaaggcac cgattactcg cagtcatcgg gttactactt
     3901 ttaacgagcg gatattttgg agctagtcat tttagggaaa tcacttcatg gatgatcagt
     3961 tatcattatc ttgatacatt agttttcttt tttccttttt taattttttt cgtttgtgtg
     4021 gtcggtactt atttgttttt ctgttataca tttcggttat ttttgatcat attgagtaaa
     4081 tggaagattt attactatgg tatcaatttt ttgataatgg gtaacacgca agttcatttg
     4141 ctcaaaagtt ggcgtacaaa ttctctgatt acagtcattc tcggaatttc tttaatgaca
     4201 attggcggca cggctagtat ctctactatt ttatcctacc aggagaaagt ttcctccccg
     4261 atggactatc agtttgatgt tgagacagag aaaaaaatca agccgattct agcagaggaa
     4321 gggcagaaga taactaaaga aatcaccttg cattataaag tcattgggag tatctatagt
     4381 caaaatctcg aagaacctat gtttcaaact gctaatctga tcacggaaac acaatatcaa
     4441 gccttcagaa aagttaatcc agatttgccg aacatttctt tgaaatcacc tggtcatact
     4501 gttttgcttg atcaaatgca aactcttttt cggaatattt cattatatgg atctagtatt
     4561 ggactgccag ataatcaaga attgaagatt caacaaatgc ttccaagtgt attaggtgac
     4621 gaaacaatgg tatatctaag tcctgtactg attgtagatg ataccgtttt tgaaaaagca
     4681 aaaggtgtgg attatcaagt agtcaatgta gatgtatcag gtggaaataa tgaaaaaata
     4741 gatgaacgac tgagcaatga cctgtcgata aagtggctca atgctgttta ttattcttat
     4801 gatatcgtaa atggaagtct acaaggagaa attagcacaa agaaacttcc ggatgaccaa
     4861 atgaaggaag cacaatttag ccaagaaagc tctcgtttga atttctcttc cagctattcg
     4921 aaattgcgtg cagttaatcg gcgaatcggt atcaatattt ttgtcacact atttgtcggg
     4981 atgatcgtga ttattgctac tggtagtatt ttgacagtcc ggcaattagc agaagcagag
     5041 gaagaaaaag aaaattatca cctattgact aagctgggga ttccacaaag aaggatcaga
     5101 cggatgatct tcgagcaaaa cgcacttacc ttctttccac ccatgatatt agggatcact
     5161 cacgcagtat ttgcaatcaa tgtcttcact cagtatataa aaactgccga ttactggttg
     5221 gcttatcttg tgagtggctt attgatcctt atctatcttt tgctttatgt cattacgagt
     5281 cagttatatt gtcggattat cgaggaatga tatacggaat aagacaagat aataaataga
     5341 actaaatttt gaatacca
//
LOCUS       DACEOE010000159         5260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEOE010000159 DACEOE010000000
VERSION     DACEOE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.162"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(392..1081)
                     /locus_tag="IW774_002300"
     CDS             complement(392..1081)
                     /locus_tag="IW774_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1154629.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1126..1890)
                     /locus_tag="IW774_002301"
     CDS             complement(1126..1890)
                     /locus_tag="IW774_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1154630.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(1918..2616)
                     /locus_tag="IW774_002302"
     CDS             complement(1918..2616)
                     /locus_tag="IW774_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1154631.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2628..3407)
                     /locus_tag="IW774_002303"
     CDS             complement(2628..3407)
                     /locus_tag="IW774_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1154632.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(3423..4367)
                     /locus_tag="IW774_002304"
     CDS             complement(3423..4367)
                     /locus_tag="IW774_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1154633.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4413..5192)
                     /locus_tag="IW774_002305"
     CDS             complement(4413..5192)
                     /locus_tag="IW774_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154634.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1385 a         1104 c          899 g         1872 t
ORIGIN      
        1 tattttttcg attctttttt tgaaccgtcc catgaacata ccgattcaac aagctaaatt
       61 ataaaaattg gtgtgctgtc attatttttt ggaaaattta ttaatggcta tcaatatact
      121 tttgtatcat actaaatagc tcgtcagttc ctatatcgga tataccaata ttaaagcatt
      181 tagatcatta tatttaagag tggtatcatc ccaaaatttc gaatattttc ttttatataa
      241 gaataatgat actgaattgc cccattcacc tttctttttt tagattttct acaagtgatt
      301 caaaccctcc ataattacag ttattacttt tgaaacgata atatgaacat gtgtaatctt
      361 ttttattaac aatccatcat tctatcccta actaatatgc tccattgggc ttgacaataa
      421 cataaaacgt ttttaaaatc aacctcaagt cgtacattaa acttcgatga ctaatatact
      481 ctaaatccaa ctcaaccatt ttatggaaac taacattatt tctatcacta acttgccaca
      541 aacctgaaca accaggtttt acatacaagc gttgaagatc atatctcgtg tactcagcta
      601 cttccctctc caaaggtggc cgtggaccaa ttagactcat atcgcctttt aaaacattcc
      661 atagctgtgg caattcatca atgcttttgg cacggataat tttacctatt ttagtgacac
      721 ggggatcttc tttcatcttg aacattgcac cttctatttc attttgatca agtaattctt
      781 ccaatttttc ttcagcatct aaacacatcg aacggaattt atacattttg aatgtttttc
      841 catttttacc aacacgtgta tggcaaaaaa atatcggact tcctggctct tcttttctga
      901 ttcgataagc tacatagaac atcaaaggag aagctaaaat caaaccaata atgcttccta
      961 caatatcaat cattctttta aaaaataaat agatatatcg tttttcaact ttttctttat
     1021 ccaaccaaac ttgattagga tgtacttcac taatacgaga taataaatct tcactttcca
     1081 tactttccct ctctcctcat ttccttttta tctacgaata aagaactaaa ataaaccaaa
     1141 cttcttcttt tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc
     1201 ctttgcgact tgtttcatct gtactacttt ttccttacca aagtccttag caatctgctc
     1261 atagcactct tttaagtaaa aggtacgctt agagacccca tgggcatcag aagcagccat
     1321 ctgcacgaga ttatgtttga ccattgtttt ggctgttttc tgaatgtctt tgccaaatac
     1381 accaacataa cttggcgcag taatttgggc caaacatccc atatctagga acggaagcaa
     1441 atgatttggg tcttctcgaa aatgcgcgtt gcgttctgga tggacgatca ctggtgtctt
     1501 tcctagttct aacaactcaa aaaaaagcca ttctgcatat aacggcactt ccagtgtcgg
     1561 aaattcgatc agaagatatg tatcatctaa atctgtaaat aaaatttcat ctcggtggat
     1621 gtcttcgatg agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa
     1681 ctgtcttttc tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc
     1741 attgctgtat tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg
     1801 aatcgctttt tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc
     1861 tggtaggata tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta
     1921 atttccgtag taataaccag catctttgct atgttctgct ccgttgtata caacacctaa
     1981 aactcgtgct tgtaccatct ctagcagttc ttttgcttta ttcaatgatt ctttacgtga
     2041 tgtattttca cgtacgacca ataaagtgcc atccgttttt gaagacatga tctgtgcatc
     2101 tgttacggct actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc
     2161 gtctaggatc tgatccattc ttggcgaact taacaattct gatgggttgg gtggcttagg
     2221 tccactaggc aaaatcgaca agttctcgac tggcgttcgt tggattacat ctgctacact
     2281 tcctgatgaa ctaagtgctg tacttaatcc cctggcattg ttcagctgga atgtcttata
     2341 gacgacgggt ttacgtaaat ctgcatcgac tagtagaact cgttgaccgg atttagcaaa
     2401 aacgactgca atgtttgctg cagtggtgga ttttccttct ccaggacctg atgaagtcac
     2461 gacaatcgtt ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt
     2521 acgatattgt tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg
     2581 agtttgtgct gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag
     2641 cggcttctgc gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt
     2701 ccttttttac ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag
     2761 attgggaatc ctaatgcatc cgtcacatat ttttcatctt tcactgtccg atcaagtaat
     2821 tcgaataaga atgccagacc tacgcctacc atcattccta atactaagcc aatcactaga
     2881 ttcaatttat tatttggcga aacaggtgtc gtattggcaa ctgcacctga aataatcgag
     2941 attttatcga cattcatcac gtcttttgcg ttctcttgga aaacttgtga agttgtgtta
     3001 gcgatctgtt gagcagtcac tgcattcgtg cttattgcct ggatcgagaa catttgtgaa
     3061 ttttgtgact gattgacgga gatcgcatct ttgatttgtc cagcagtcat tttcacattg
     3121 tcttctgttt ctaaacgatc tttgacttca tttagtacta aatcactgac gatcatatct
     3181 ttatacgtat tgatcatttg caggttcgta ttgacatcat tgacatttgc tgtgtctgat
     3241 tgtggcagtg tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat
     3301 gttacgattc ctgagatggc caagccagca aacattgaga tgatgattgt taccaagtgt
     3361 ttgcgtaatg tttcaaaaat ttcttccaag ttgattgtct cttccatttt ttctccactt
     3421 tcctatttat tctctagatt catttgatct tttaattctt tttttacacg atcaagttct
     3481 tgatcactga ctttttgata ggagatatta tcttgcataa agccttctcc ttgcagctga
     3541 tcttgcttga tcgtaccaaa tgcctcccga taatctaaag cgattttttt catttcatca
     3601 aagctcatgt ctgttttcat attcgactct agagcattca aaacggattg atagttgctg
     3661 acaccgttta aactcaatac tttttttgct acaccttcta cgatctttct ttgacgctct
     3721 tgtctaccat agtcaccatt tggatcttca taacgcatac gagaataaga taatgcctga
     3781 tccccgttca atgtgatttt accgattgta aagtcatagc catcttgtga gaaagtgagg
     3841 tcattattga cttcgatacc accaactgca tcaacaagtt cctttagccc tttcatattg
     3901 atagagacat agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg
     3961 gcagctccgc cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg
     4021 tcgacatacg tatcacgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact
     4081 atggcaacca tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca
     4141 atccccatca atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt
     4201 tctccttggg ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga
     4261 tataattttg ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata
     4321 atcagaaata tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt
     4381 tgctaacctt gcttgaatca acgttttaac tgtcaataac ttatattgct atagcccatt
     4441 tgctgagcaa cagtttgtgc ttttgttgcg tatgctgcgc ttattttttc attccggatc
     4501 tgctctattt gactgagcgc cgtcaaatat tgatcgtaag tagcggtggc agtcacttga
     4561 ccatccttca acatcgtatc gaataactga tctagttgcg tgacttgcgc gatctgagta
     4621 tcagcgaatc ctaaatattg cataacaacc gttttccaag gactatcttt aaaaaagctc
     4681 atattttcac gtacacgagc gacatctgca cttgtcacct tttctttgat gatgacatcg
     4741 tctacagctt gctgccagtt tgatacattt ttagtaaaca aatcgttgac agctgtctgg
     4801 atcttcagtt ttgattctgc actatctaac tgtgtcacga ttgctttctt ctcatcttgg
     4861 attgcaacag cttctttagg caattctgat tctttgatgt cgtaatcatc tgctgaaagt
     4921 ttgacatagg atagcttatt tgacaaactt gtgattgttt cttctgttat ttcagggttt
     4981 agaaaaattt tttcttttgt actatacagc ttgtctactt cttttgataa agaagtcaag
     5041 gtgtcttttt gattggtgat ggcagattct gcataattga tccgttgctg atgttgccgc
     5101 tgattattga tatggtaacc aaacaaacag atcgcaatag cacctgcacc taccgccaca
     5161 gcagtcaatg acttgacgaa tggcttttcc atagattcac tcctaagtat tgatgataga
     5221 taaattaaga tataattcac ttacataaaa atgacagctt
//
LOCUS       DACEOE010000160         5089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEOE010000160 DACEOE010000000
VERSION     DACEOE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.163"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..182)
                     /locus_tag="IW774_002306"
     CDS             complement(<1..182)
                     /locus_tag="IW774_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1154635.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="IW774_002307"
     CDS             517..951
                     /locus_tag="IW774_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1154636.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="IW774_002308"
     CDS             974..1294
                     /locus_tag="IW774_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154637.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="IW774_002309"
     CDS             1435..1626
                     /locus_tag="IW774_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154638.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="IW774_002310"
     CDS             complement(1697..1954)
                     /locus_tag="IW774_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1154639.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="IW774_002311"
     CDS             2112..2375
                     /locus_tag="IW774_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154640.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="IW774_002312"
     CDS             2399..2587
                     /locus_tag="IW774_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154641.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="IW774_002313"
     CDS             complement(2670..2855)
                     /locus_tag="IW774_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154642.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="IW774_002314"
     CDS             3070..3441
                     /locus_tag="IW774_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1154643.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="IW774_002315"
     tRNA            complement(3591..3663)
                     /locus_tag="IW774_002315"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="IW774_002316"
     CDS             complement(3709..4137)
                     /locus_tag="IW774_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154644.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="IW774_002317"
     CDS             complement(4197..4955)
                     /locus_tag="IW774_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1154645.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1776 a          785 c          991 g         1537 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattc
//
LOCUS       DACEOE010000161         5088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEOE010000161 DACEOE010000000
VERSION     DACEOE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.164"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            21..839
                     /locus_tag="IW774_002318"
     CDS             21..839
                     /locus_tag="IW774_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154646.1"
                     /translation="MKEEDKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFN
                     SRNYTNENMELLRKDFEVVYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQF
                     VRTLWADGQVFLPERIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKS
                     LSLINSEQAGRQEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDE
                     CLCAKKRVVLCDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1060..2823
                     /locus_tag="IW774_002319"
     CDS             1060..2823
                     /locus_tag="IW774_002319"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1154647.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2907..3365
                     /locus_tag="IW774_002320"
     CDS             2907..3365
                     /locus_tag="IW774_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1154648.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3474..4157
                     /locus_tag="IW774_002321"
     CDS             3474..4157
                     /locus_tag="IW774_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1154649.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4304..4918
                     /locus_tag="IW774_002322"
     CDS             4304..4918
                     /locus_tag="IW774_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1154650.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
BASE COUNT         1802 a          810 c         1050 g         1426 t
ORIGIN      
        1 gaaaggcatt tccccttgga ctgaaagaag aagataagaa aagcgcagaa acgaagccgt
       61 tatttcaatt agtctgtcaa ggattggaag agcaaaaagg aattctagac ttacaagaag
      121 aagatgtcta ttatattttt tctttgttca acagtagaaa ttataccaat gagaacatgg
      181 agctgttaag aaaagatttc gaagttgttt ataaaaattt tgttctcaat gacctatcac
      241 tgaatgaatt gattttacag attattagta tcgctgaaat cgacgatgca gaaaatttta
      301 ttttcaagca atccttttta caatttgtcc gtacgttgtg ggcagatgga caagtatttt
      361 taccggaaag gatctatctt ctatcagaaa aagaacaact tctgtacgga aaaattgtag
      421 aaatattgga agagtggaaa aagaaaaatc aaatccaact aagatggaat gaaaatttca
      481 ttcgaaaatt cgtcaaaagt ctttctttga tcaacagtga acaagcgggg agacaggaaa
      541 tagaaatttt tgttgtttca gaaagttcag tcaaacaatt tttttatcga acccaatttc
      601 atttgtatgc ggaagaaggc atcgctgaaa taaatcctat tttatttcgt tcattagaag
      661 aattgcctga tgaatgtcta tgtgcgaaaa aaagagtggt tttgtgcgat gctgctagct
      721 atcaaaatgg attagaaact gaaaagacaa agatctatcc aatctcatta aaaggtgcaa
      781 acatccattt gtatcatatt gctcaggaac ttcacctatt aaaaaaacat atttcttgat
      841 gagaccttaa ttaggagacc ttctacaaag ctttttaatt gtctgtagaa ggtttttatt
      901 tgagtcctga ttcgtgcgaa gatctactta tataacaagc tttctctgaa atttttttaa
      961 aatgctattg acttagagtc aactctaagt agtagaatag ataaagtgta acaaatctat
     1021 tttcattaaa agaagatagt acggaggaga gaatgtatta tgagtacagt attacatgta
     1081 ttggatgcaa tcaatagccc cgcagatgtc aaaaaattat ctgttgagga aatgaaacag
     1141 ttggcagaag agatgcgcca aaaaatcttg caaaaggaca gcgatgtagg tgggcacgtt
     1201 ggtccgaatc taggtgtagt agaattaacg attgcgtttc attatgtatt tgattcacca
     1261 aaggataaaa tcgtttggga tgtttcccat cagtcttatg gtcataagct tttaactggt
     1321 cgaaaaaatg gttttgaaaa aggacattac cgtgaagtga gcggatattc caaccaacat
     1381 gaaagtaaac atgactattt tacagtgggg cacacatcta catctatcag cttagcaaca
     1441 ggtttagcta aagcgagaga tctgaagaaa gaatcaggaa atgtggtagc atttatcggt
     1501 gacggctccc taagtggtgg cttagctttt gaaggattga acaatggtgg tgttttacaa
     1561 tccaatctaa tcgtattagt aaacgataat gagatgtcta ttgatgagaa tcatggcagt
     1621 ttatataaaa acttagctga gcttcgtcaa accaacggta aaagcagcaa taacctattt
     1681 aaagcaatgg gatttgatta tcgttacgtg gataatggaa acgatctgga aacggtgatc
     1741 cgtttatttg aagaagtaaa agatattgat catccgattg tacttcacgt ccatacggaa
     1801 aaaggtcgcg gttatcagcc ggcaatcgat aataaaatga aatatcattg gcatgtacca
     1861 tttgatctgt ccaccgggga ggcaaagcaa gtaacttctg gagaaacatt taatatgatc
     1921 atcatggatt atttggataa gaaaatgcaa acaagtacag ctcctattgc cgcagtcaat
     1981 gcagcgatac caggcgcctt tggactgaaa acattccaag aaaaacatcc agatcattat
     2041 tttgacgtgg ggatcgctga acagcactct atcactttcg cttctgcttt ggcttctcag
     2101 ggagtacgtc cagtcgtctt ccaatcaagt actttcttgc aacgggcata tgatcaatta
     2161 tcacatgatt tagcgatcaa cgagaatcca gtgattattt tagtcggtgg cggaacgatc
     2221 tcagctagtg accgaacaca ccaaggaaca tttgacattc cttacttatc aagtatcccg
     2281 aacatcaaat atcttgctcc agcaacaaaa gaggatatga tctctatgct agattgggcg
     2341 ttcgaacaaa cagcgcagcc tgtagcaatc cgaattccta cacatggtgt agaacatggt
     2401 acagctatcg caaatgatta tagtattcca gcctatcaaa cagtaaataa tggaggaaaa
     2461 gtagcaattt tagctttagg cggttttctt gaattaggaa aagaaacagt aaaagctttt
     2521 agaaaaaaag gaatcgaagc aacgttgatc aatccaggaa cgatcacaga aaatgacgta
     2581 gaaacattag ataaattaaa agaaacacac gaattagtca tcacattaga agatggtagt
     2641 ttgtcaggag gatttggcga aaatatcagt cgttattatg gtgattcttc aatgaacgta
     2701 ttaaattttg gtgcagagaa agaattcacg gatagcgttc ctgtagggca gctttatgaa
     2761 cgttatcatt taacaccaaa acaaatcgtt gaagatagtc tgcgtgtctt gaatattcaa
     2821 taattcatta tgctcaccat ccagataatt gtttatacta gtatctggat ggttttttca
     2881 ttataaaaag caaaggggtg gatgttatgt atcagcaagt agaagaatat gtcaaaaagt
     2941 tccaagaaaa atatcctagt tatcaatcag aaaaattagg tttcttttca tttggggata
     3001 cagaaaaaat ggcagacgag ttgagcgaac tagttgtaaa aaagataaag actggtacat
     3061 caagtggtta cgatctgtat aaacaagaga atgagccttt accaaaagtt ggtcaattag
     3121 acgtggtcct gaatggaaaa aatgaaccag tttgtatcat caaaaatata aatgtgacca
     3181 tcttgccttt tagtgaagtc acgaaagagc atgcttggaa agaaggagaa ggggatcgga
     3241 cattagctta ttggcggaaa gtccatgctg agttttttaa acatgcttat aaagaagcgt
     3301 tgaacataga atttacagaa gagaagctgg tcgtatacga agaatttgaa gtaattttct
     3361 tttagtaaga aaggtctgct gaaagaaata gctcattatt gtctcctgaa tagaaaaggg
     3421 ttacattttt atatgaagct gtatttttat ataacattac tggaggagat attgtggata
     3481 cactggaaca aatcaaaggc ggagtcgtcg tttcttgtca agctttggaa aacgagccgc
     3541 tacacagttc ttttattatg gggagaatgg ctttggctgc aaaagaaggc ggagcagttg
     3601 gtattcgagc aaatacgaca gaagatatca atgaaatcaa gcgaatcgtt gatttaccgg
     3661 taatagggat tatcaaaaga gattacgatg ggtcagaagt gtttattact gcgacagaaa
     3721 aagaagtgga agaacttttg gagacgcaag cagaaatcat tgcactggat gcaacttctc
     3781 gtaaacgtcc gacagaaaaa acaactgctg aacttgtcca aatgattcac gaacacggtc
     3841 ggttagctat ggcagatatt tcgacttttg aggaagcgct tcaagcgcaa gaagatggtt
     3901 ttgatctttt gtctacgact ttagcaggct atacagaata ttcacgaaag acgcagacac
     3961 ctgattttga tttattgaat caaatcatag aacaagtcga tatcccagta attatggaag
     4021 gacatactga ctctcctgaa caagtagaga aagcatatga attaggtgct ttttcagtcg
     4081 tagttggatc tattatcacc cgtccgcaat tgatcactaa aagatatgtc gaagccgcaa
     4141 gtaaagcaaa taactaaaaa acacgcacgg cacagattct tgacaaaaaa gaaaaattga
     4201 tagatagttt aaagcagatt ctaagaagat gaaaaaagca ttacttttca ttttgtcttc
     4261 aagatctgct ttttaacgaa ttttatagaa agaaggcgaa accatgtttg aacggagtcc
     4321 ttcagaattg acagaacgtg aaaattacaa gttgctgatt ggttcagtca tccctagacc
     4381 agtagcagta gtaacgacta aatcagcaaa agatatagtg aatatcgcac ccttcagtta
     4441 cttcaatatc gtcagttcga atcgacccat cttatccttg gcgatccaga gaaaacaaga
     4501 tgcagtaaaa gacacagcaa agaatattct tgagacaaga gaagctgtta tccatatcct
     4561 tgatgaggac aacgtagaag aagtaaataa aacagcagcc aatctgccta atgaggaaag
     4621 cgaactgtac ctgactgact tgactttaac tgattcagta atcatttcag taccagcatt
     4681 gacagaagca aaagttcgtt ttgaaacatc cttatatgaa catgtcgaga tacggaataa
     4741 aaataaagta acagctgatt tatttttgct agaagtacag aaatatcatt taaatgaaac
     4801 agtctatgat gataagacag gaaggatcga tccaagaaaa ttgaatgctg taagccgttt
     4861 ggcaggaaat gattatgcag caataggaga aattttcacg attgcacgtc ccgaataaaa
     4921 aggagcacta gttatgttaa aaataagcta taaaccatcg acagattcaa aagaaatgaa
     4981 aaaagaatat gaaacagtga atgattttct gcaagggcag tacttagaag ttccgccatt
     5041 gcaagaccat tttgtcgtta ctactgttac tttagatggt aaggaaat
//
LOCUS       DACEOE010000162         5079 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEOE010000162 DACEOE010000000
VERSION     DACEOE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.165"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            140..1183
                     /gene="hrcA"
                     /locus_tag="IW774_002323"
     CDS             140..1183
                     /gene="hrcA"
                     /locus_tag="IW774_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1154651.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            1277..1840
                     /gene="grpE"
                     /locus_tag="IW774_002324"
     CDS             1277..1840
                     /gene="grpE"
                     /locus_tag="IW774_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1154652.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            1891..3720
                     /gene="dnaK"
                     /locus_tag="IW774_002325"
     CDS             1891..3720
                     /gene="dnaK"
                     /locus_tag="IW774_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1154653.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            3871..5037
                     /gene="dnaJ"
                     /locus_tag="IW774_002326"
     CDS             3871..5037
                     /gene="dnaJ"
                     /locus_tag="IW774_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1154654.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         1736 a          902 c         1160 g         1281 t
ORIGIN      
        1 tcaattattt agaattagca ctcttgtgtt tagagtgcta aaaaatggag tctttctctt
       61 gacaggagaa gggcttcttg ctatatttat aagtgaatta gcacttagaa tatatgagtg
      121 ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac gacattttac gtctgatcat
      181 ccaacactat acgagtagtg gcgtgcccgt tggttccaaa acattaatgt ctgagggagt
      241 caaggccagc tcagcaacga ttcgcaatga tatgaaagca ttagaagatg aaggattgct
      301 gttgaagact cactcttctt ctgggcggat tccatctgct ttaggctatc gttattacgt
      361 ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat ttgctgctga ttcgtcagtc
      421 tctaggcaga gaatttcatg aaatcaatga gattattaag caatctgctg agatattatc
      481 tgagctgaca agctatacga ctttttcatt gggacctgaa ataaaagacc gccgactgac
      541 tggctttcgt atcgtaccct tgaataatcg acagattatt gctatcgtcg taacagatat
      601 ggggaatgta gaaagccaag tgttcactct tcccgaatat atgggaagtc aggaactaga
      661 aaaaatggta cagattgtaa acgaccgtct agtcggagat cctctgatga cggtgtacca
      721 taaacttcgt acggagattc cgatgattct tcataaatat ttccagacga cagaagggat
      781 gtctcagtta tttgacaccg ttcttggtca tgtttttgaa gaaaaggttt atgtcagtgg
      841 acaaatgaat ctcttggatt atgagccccg tcaagatctt gatcagttca aatcgatgtt
      901 ttcttttatg aaagactcag acggacttac acaaatgatc actccgatgg atagcaatat
      961 tcatatcaag attggtgctg aattaggcaa tgatttattg cagaatatga gtatgattca
     1021 agcaagttac gagatagatg gtcatggaag aggtacaata gccttgttag ggcctactag
     1081 tatgccttat tctcggatgt tcggtctagt cgatgtttat cgaagagaac tagcagctaa
     1141 acttgctgat tattaccgct cgcttgactt gtcaaactcg taatgtgaat aacgaagcta
     1201 tttgcagctg atcgttctct ctttcttagt aagagcaatc aaacagctga ctgaataatg
     1261 aaaggaagac tgtatcgtga aagaaaatca agaagaacta gataaagaaa tgacagatgc
     1321 tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat tcgggtattt cagaagctga
     1381 agcggaagaa ttcgaaacag cgaaattaaa agctgagtta gaagaaatgg aagatagata
     1441 cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga ggaaaaaatg agcgtgagca
     1501 attacaaaaa taccgttcac aagatttagc caaaaaatta cttccatcaa ttgacaacct
     1561 ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg gcaggtttga aaaaaggcgt
     1621 ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa gaaggaattg aaaaaatccc
     1681 tgctaaagga gaagcctttg atcctaatct tcatcaagca gtacaaactg ttccagcgac
     1741 tgaagataca ccagccgata cgattgtaga agtactacaa gaaggctaca aattacatga
     1801 tcgcgtgtta agaccaacta tggtcattgt tgcacaataa ttgtgcggat ataaaactta
     1861 cactttcaat taaagaagga gacgttaatt atgagtaaaa ttatcggtat tgacttaggt
     1921 acgactaact ctgcagtagc agtattggaa ggaaacgaag caaaaatcat cgctaaccca
     1981 gaaggaaacc gtacaacacc ttctgttgta tcattcaaaa atggagaaat ccaagtaggg
     2041 gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga tttcatcaat caaacgccat
     2101 atcggtgaag caggttacaa agtagatgta gaaggtaaat catatacacc acaagaaatc
     2161 tcagcaatga tccttcaata tatcaaagga ttcgcagaag actatttagg tgaaaaagtc
     2221 gaaaaagcag ttatcactgt gccagcttac tttaatgatg cacaacgtca agcaacaaaa
     2281 gatgctggta agatcgctgg tctagaagta gaacgtatcg tcaatgaacc aactgcagct
     2341 gctcttgcct atggtttaga taaaacagat cgcgacgaaa aaatcttagt attcgacctt
     2401 ggtggcggta cttttgacgt atctatcctt gaactaggag acggtgtctt tgatgtattg
     2461 tcaacagctg gcgacaacca tctaggtggg gacgactttg ataacaaaat catcgactac
     2521 atggtagcag aattcaaaaa agaaaacagc attgacttgt ctcaagacaa aatggcttta
     2581 caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt tatctggcgt atcaagcaca
     2641 caaatcagct tgccatttat cacagcagga gaatctggac cattgcattt ggaaatgaca
     2701 ttgacacgtg caaaatttga tgaattaaca gctgacttag tagaacgtac aaaagtacca
     2761 gtacgtcaag cgttaaaaga tgcaggtctg tcacaatcag aaatcgacga agttatttta
     2821 gtcggtggat ctactcgtat ccctgcagtt gtagaagcag tacgtaaaga aacaggaaaa
     2881 gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta tgggtgctgc tatccaaggt
     2941 ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg acgttacacc attgtcatta
     3001 ggtatcgaaa caatgggtgg agtgttcact aaattgattg accgcaatac aacgattcca
     3061 acaagtaaat cacaagtatt ctcaactgcg gcagataatc aaccagcagt agacatccat
     3121 gtattacaag gtgaacgccc aatggctgct gacaacaaaa ctctaggaag attccaattg
     3181 acagatattc cagcagctcc tcgtggtatt ccacaaatcg aagtaacatt cgatattgat
     3241 aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta cacaaaaaga acaaaaaatc
     3301 acgatcaaat cttcttcagg tctaacagat gaagaaatcg aacgtatggt gaaagatgca
     3361 gaagcaaacg ctgaagcaga taaagctcgt aaagaggaag ttgacctacg taatgatgta
     3421 gacgcattgc tattctctgt ggacaaaaca ttgaaagaat tagaaggcaa agtggacgaa
     3481 gaagaagtga aaaaagcaga aacagctcgt gacgaattaa aagccgctgt tgaagcaaac
     3541 aacatcgagg aaatgaaaac taaacgcgat gcattgaacg aaatcgttca aaacttgact
     3601 gttaaactat acgaacaagc tgcacaacaa caagctcaag aaaatccaga agcagctcaa
     3661 ggcggagcag atgatgtcgt tgatgctgat tttgaagaag tcgatggcga cgataaataa
     3721 tcagttcact gaatttcgag agaaggccaa agttgctact ttggcttttt cttttgaaaa
     3781 atagtttgaa gtatcggttt ttgtttcaaa caaaatatga tatgataaca agcgacgtga
     3841 aaatgatgag aaccaatagg aggaaagcct atggcaacaa aacgtgatta ttatgaagtc
     3901 ttagggctct caaaaggagc atcagaagat gaaataaaga aagcttatcg taagctttcg
     3961 aaaaaatacc atcctgacat caataaagaa cctgatgcgg aagaaaaatt caaagaagta
     4021 tcagaagcat atgagatttt aagtgacccg caaaaacgcg ctgcatacga ccaatacggg
     4081 catgcaggca cagatccaaa ttacggtgct ggtggagctg gtggcggatt tggtggattc
     4141 ggcggcttct ctggtggcgg cttcggtggt tttgaagata tcttcgactc tttctttgga
     4201 ggaggcggtc gatcagtcga tccaaatgcg cctcgtcaag gtgctgattt gcaatatacg
     4261 gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa aagaaatcaa gtataatcgg
     4321 gaagaaatct gtcatacttg tggtggaaac ggtgcaaaac caggcacaca accaactact
     4381 tgtcataaat gtcatggtgc aggtacgatc aatgtagaac gccaaacgcc acttggacga
     4441 gtgatgagcc gtcaaacttg tgatgtttgt catggcacag ggaaagaaat caaagaacct
     4501 tgtccaacct gtcatggctc aggacatgag aagaaagcac attcagttaa agtgaatgta
     4561 ccagctggtg tagaagaagg acaacaaatg cgtttagctg gtcaaggaga agctggagaa
     4621 aacggcggac catttggtga tttgtatgtg gtctttcgtg tagaagaaag tgatatcttt
     4681 gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa gttttgtaca agcagctttg
     4741 ggggatgaag tccaagtacc aactgtccat ggagatgtga aattgaagat ccctgctgga
     4801 acacagacag gtacgaactt ccgtttacgt ggaaaaggcg ctccgaagtt acgtggtggt
     4861 gcaacaggcg accaacatgt caaagtaaaa ctgatcacac cgaaaaatct aaatgaacaa
     4921 caaagagaag cattacgtgc attcgctgaa gctggcggca tcaaagttga agaacagcaa
     4981 gaagacggtt tcttcgataa gtttaaagat gcttttggcg ggaaaaagcg gaagtagttt
     5041 ttctagtaaa aatataaagt agacttgccc aaattttct
//
LOCUS       DACEOE010000163         4958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEOE010000163 DACEOE010000000
VERSION     DACEOE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.166"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..2118
                     /locus_tag="IW774_002327"
     CDS             100..2118
                     /locus_tag="IW774_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1154655.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            2545..>4958
                     /locus_tag="IW774_002328"
     CDS             2545..>4958
                     /locus_tag="IW774_002328"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1154656.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT         1713 a          924 c         1062 g         1259 t
ORIGIN      
        1 tgattacaaa aaacaacaaa gctcttaaat aacttttaat aaatattaag agaagttata
       61 attattaata atagataaat tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa
      121 gaacggagac gtttagaaca gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc
      181 gtgggaactg cgatggtagg ctgttctgtc gctgccccat tagtacagcc ggttcctgtt
      241 caagcggatg aaacacctgt tcagttcggc gcaagaatca atacagcggc atttatcaca
      301 gaaattgcta cttatgctca accaatcgct caagccaacg atctatatgc atcagtgatg
      361 atcgcacaag cagttgtaga aagcggttgg ggcagtagtg cgttatctca agcaccttat
      421 tataatcttt ttgggataaa aggcagctat caaggacaaa ccgtctacat ggatacgctt
      481 gaatttttaa ataatcaatg ggtaaataaa aaggagcctt tccgacaata tccttctttt
      541 gctgaatctt tcaatgacaa tgcctatgta cttagaaata cttcatttgg caacggttat
      601 tactatgccg gtacttggaa gagcaacacg aaatcctata cagatgcgac agcttgtttg
      661 acagggcgtt atgcgactga tcctggttac gcaggcaaat taaataatat catcgcaact
      721 tacggattga cccaatatga tacaccagct agcggaaacg caggcggtgg agcgactaca
      781 ggtggcaata atactggaag tgcaggtagt actggcggtt caagcaattc tggtaataca
      841 aactcaggtt cagcaacttc gaccacttat actgtccaat ctggtgattc tgtttgggga
      901 atctcaaata aattcggtat cacaatggat caattgatcc aatggaacaa catccaaaac
      961 aatttcattt atccaggaca aaaactgaca atcaaaggta gccaggaaaa tggctcttcg
     1021 acgaataatt ccggtaacaa taccaactct tcaggaaatg ctggtacatc aaacggagga
     1081 caaacaactg gtgcgaaata cactgttcaa tctggtgatt ctgtttggaa gatctcaaat
     1141 gatcacggaa ttacgatggc tcaattgatt gaatggaaca atatcaagaa caatttcgtt
     1201 tatccaggac aacagctaat cgtatcaaat ggcggttctt ctaatgctgg agcaaatact
     1261 ggctcaaata ctgggaatac aagtggctct agttcgtcca gctcaagttc ttctaataca
     1321 gcttcaggaa cgaaatatac agttaaagcc ggagactctg tttggagcgt ctcaaataaa
     1381 tacggaatta cgatggctca gctaatccaa tggaacaata tccaaaataa ctttatctac
     1441 ccaggacaac aactgactgt ttcaaacagt ggatcaacaa acagcacgtc aggtaataca
     1501 ggttcgtcaa ccaatacaaa taacagcagt tcagttaata ctggcagcag ttcgtctcaa
     1561 ggcacttata ccgtgaaagc aggtgaatct gtttggagcg tggcaaataa aaacggcatc
     1621 acgatggatc agttaatcga atggaataat atcaaaaaca acttcattta tccaggacag
     1681 caattgatcg ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca
     1741 gcgaaaccaa atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg
     1801 atgtataccg tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg
     1861 atggatcagt taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa
     1921 gtgattgtga agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa
     1981 acctatactg tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca
     2041 atggctcaac taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg
     2101 ttgattgtag ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata
     2161 tttgttacta taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact
     2221 gaagtttcag agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt
     2281 gagttgccct tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg
     2341 aggccttttc actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag
     2401 caatcttttt ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg
     2461 aaataaaaat aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt
     2521 ttcaatagaa caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg
     2581 caaaaatact gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa
     2641 ttttacgcac tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct
     2701 gaaggttata cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg
     2761 ctgcatccaa tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca
     2821 ggaaacgatc cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat
     2881 tctttaggat ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat
     2941 aaatggacac aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa
     3001 gtagcggtaa actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat
     3061 gggaaaagtg aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg
     3121 aaaatcactg cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa
     3181 agcattaaag aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt
     3241 aaagtggctg gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc
     3301 ggtgcaactt actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct
     3361 gaacaaaaag ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag
     3421 agaacggatc ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca
     3481 gttaatggca aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca
     3541 ggagccatca tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc
     3601 ggactagaaa ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa
     3661 gatggtacgc acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat
     3721 aaaatgaatg aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta
     3781 cgtgactggc tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg
     3841 gaggacggaa cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca
     3901 aaaaacatca agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc
     3961 aatgtcgttg atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa
     4021 tgggcaggaa gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta
     4081 gcagattatg aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa
     4141 catgcggtac ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt
     4201 gttgttccta caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa
     4261 aacaacgaaa aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa
     4321 acttacggtg cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg
     4381 attgcttgga gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt
     4441 ttgatcgttg atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag
     4501 cttgataaag tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat
     4561 ttcaatacag caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct
     4621 ctgccttatg aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg
     4681 ggtgaagaac tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca
     4741 acatacgatg aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt
     4801 aaagttcgtg cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca
     4861 gcattagcca atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt
     4921 gcggtaccaa acaagttagt caatatcgtt gctaatta
//
LOCUS       DACEOE010000164         4940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEOE010000164 DACEOE010000000
VERSION     DACEOE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.167"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            212..1012
                     /locus_tag="IW774_002329"
     CDS             212..1012
                     /locus_tag="IW774_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1154657.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1031..2899
                     /locus_tag="IW774_002330"
     CDS             1031..2899
                     /locus_tag="IW774_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1154658.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            2892..3383
                     /locus_tag="IW774_002331"
     CDS             2892..3383
                     /locus_tag="IW774_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1154659.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3371..3925
                     /gene="srlA"
                     /locus_tag="IW774_002332"
     CDS             3371..3925
                     /gene="srlA"
                     /locus_tag="IW774_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1154660.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            3943..4938
                     /locus_tag="IW774_002333"
     CDS             3943..4938
                     /locus_tag="IW774_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1154661.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1725 a          771 c         1035 g         1409 t
ORIGIN      
        1 ttcttaaatg ataaaacaat tatatatcaa aaagagaaac attgcttata taacgctcag
       61 gaattttagc aatctatgca aaaagatgaa aatttttttt gctcttccat tagtacacat
      121 tttttagctg aagctgtaaa tttcatgtct taattttttt tagtgagctt ttatactgga
      181 tttgtaagaa aaaagaagga gaggatcaca tatgtcggat tggttggata ttgcaggaaa
      241 agtagtaatt gtaactggag gttcctcagg aattggaaga agtatcgtcg aaaatttgtt
      301 gaagcagaat gcacaagtag cgaattttga tgtaactgaa tgccgtatac agcatgaaaa
      361 cctactatct ttaaaagtcg atgtgtcttc taaaacggac atagaagaag ggatatataa
      421 agtaatgaaa catttcaaga cgatcgatgg tctagtcaat aatgcaggaa taaatattcc
      481 ttctcttcta atagatagaa atcatccaaa aagcaagtat gaattatcag aacaagtttt
      541 tgataagatg atcgctgtca atcaaaaaag tgtatatttg atgagccaag cagttggaag
      601 aatcttggtg caaaagggat caggagtgat cgtgaatctt tcttctgaat caggattgga
      661 aggatcagaa ggacagagct gttatgctgc tacaaaagca gcgatgaaca gttttactcg
      721 ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg gtaggtgttg caccagggat
      781 actggaagaa accggtttga gaacacaaga atatgaagaa gctctttctt atacgagagg
      841 tataagcgtc gaacagctga gaaacggtta cagtcgaaca agtacgatcc ctctaggaag
      901 gagtggtaag ctgcaagaag tggcggatct agtctgctat tatctttcag aaagatcaag
      961 ttatatcact ggagtcacga ctaatatttc aggaggtaaa acaagaggat aaaggatagg
     1021 ggggacgcac gtgacgctag taaacagatg gtaccaatta ttgaaattac tagtcactca
     1081 caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa accagtaatc aaaccataaa
     1141 aaaagatatt ggggagttga atgaagaaat tgccggtatt gcaaaaatca tccaaaaaaa
     1201 caaacatttt gaacttgaaa tttatgattt tgattttttt gatgagatca tgcaaggaaa
     1261 attgaaaaca gccagtgatt tcaattcggc atccaaaaga atcgcttatt tactaaatag
     1321 gctgatcgaa acagaggatt tcttacgtat ggatgatttg tctgaagaag tcggtgtcag
     1381 cagaggaaca gtggtcaagg atctaaagac attaaaagag atgatcaatg attttgatgt
     1441 aaagattaca ggcacaccaa ataaaggact aagaataata ggagatgagc tagaattacg
     1501 actactttta ttttattttg tttctcccta cttttcagag acgaaaacag aacagtttct
     1561 agaagaaaaa caagcaatca aacattttca gaagaagacg aatgctagta aacaagaaat
     1621 caccctttta acgaaagtta tcggtatttc tttgaataga atctctagta actatctgtt
     1681 gaagaagccc atcaatcgtt acagtgggtg ttttgaatat atccaagaat tcaatgaact
     1741 gatcaatcag ttggaagaaa tctatgaact aactttgagc caatatgaaa aagattttat
     1801 cagttttcct ttgaacatca tcaacacaag tataacgatg ataaggaaag ctaactctga
     1861 tttgcgctta tatttcgacc ccatgatgat gcgtattcga cgattagatc tcattgatct
     1921 ggatgagtcc ttcttatatc aagagatgaa acagcacctc tctttattga taaatcgggt
     1981 aatttttcgt tatcgattga ctgatttatt ttatggtgag atagaaaaaa agtatccttt
     2041 ttcttaccaa atcgctagcg aatgcataca agcattagaa gctatcttgc aacataagat
     2101 ctctaaagta gagatcagct acctaacgtt gtatttggag ttagcatttc gatatcaaaa
     2161 agagcatttt tcctctaaaa aagtagccat tgttcatacg acaggaaaag gaacagcatt
     2221 aatgatccaa agacaaatca aaaggatatt ggggagggat atacaaataa cacatgtttc
     2281 agaagaacaa tatgaaaaag tagatctaaa caaatatttc gctgttttta caacgattcc
     2341 gttaaaaaat gtaaatgaag agacaccggt gatccatttg actaatcttt ttgatgactc
     2401 ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga ttagccgggt ttcattctac
     2461 tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat caagaatatt taaacaaatt
     2521 agtcgctcct ttgatcagag gctattatgt ggatgaagaa tttgaaaaaa ggatcttgaa
     2581 aagagaatat cagcaaagca catttgttaa caaagaattt gcttttcctc acacaaccaa
     2641 ccagctatct caacacattg ttttatcagt agggctaata actgatcaag gtttagcaga
     2701 aacaggagga aatggagtta tttttttatt tgccatacct gaagaaatga cggctgaagt
     2761 tgagaatgaa ttatttgagc tatacgatat cctatttgaa ctcataaaaa tgggaataga
     2821 tgagtttatt gaattagcac cgacacaaga atcattttta aaactaatca aaagtaaagg
     2881 agagagactt catgaatgat atgtatctat tcatagtttt cgcagcttca gcttatgtgc
     2941 tgcaagcagt tatcagttgg aagcaactaa agcatttcaa tcaaacatat agcgaactgc
     3001 gaagaaaagg aaaagtagcg attggtaaac gacctgggaa aatccgttca ggcacgattg
     3061 ttatgctagc tatagacgat catgatcaag ttttagaagt cagaaaaatg caaggggtta
     3121 catctttggc aaaatttcat tcattacctg tctatgcggg tgaagatatc cattacatcg
     3181 atcgttacca tccctcagtt cgagcggaag atcgtttgac acaagcagct ttagaaaatg
     3241 ccagagaagt atacttacga atagaggcag gaaatgaaaa aagtgaacaa ccttcattgc
     3301 cttttattgc tatgctcaat aattttcgga tatggaaaag tacgatcaaa gaaaaaagga
     3361 gtgacaaacg atggatatca taacaaaatt tgctgaggga tttatgaggc tgttccaaac
     3421 aggtgcagaa acattcataa gctggatgac aggaatcgta ccagttgtct tgatgctgat
     3481 ggttgcaatg aatacgctga ttgctttttt aggtgaagac cgagtaacga aagtagccaa
     3541 attatcagcg aaaaatccgc ttactcgtta ccttgtgtta ccgttcttat ctgcttttat
     3601 gttgggaaat cctatgtcat ttacaatggc tcgtttcctt ccagaatact acaaaccaag
     3661 ctattatgct gctcaagctc aattttgtca tacgagcaat ggtgtattcc cccatattaa
     3721 tcctggagaa ttgtttgtat ggatggggat tgctcaaggg atcgaaacct tagggctaaa
     3781 ttcgatggag ctggctatcc gatacttgct tgtgggactg ctgatgaact ttattggtgg
     3841 ttggataact gattttacta ctggatttgt atgcagacag caaggaatcg ttcttagcaa
     3901 aaaagtagag ctttcagttg attagagaaa ggagagtttt aaatgtatgg tgatattaag
     3961 atcgtaagag gaaacggcgg gtatggaggt cctttgatta ttaggccgac aaaagataaa
     4021 cacaagttca tctatgtcac aggtggaggt gagaggccaa tgatcgtaga taaaatagaa
     4081 gaactgacag gtatggagtc tatcaatggg ttcaaaacat caatacctga tagtgaaatc
     4141 gcgcttgcaa tcgttgactg tggagggact cttagatgtg gaatttatcc taaaaaaggg
     4201 ataccaacca tcaatatcgt tcccacagga aaaagtggcc cattagcaca atatatcact
     4261 gaagatattt atgtttcagc agtagggtta gaacaaatac agccactaat ggaaactgat
     4321 gataacaaag aaacagtaaa tactgcatct ccagataaaa agtacaaata tgatacttct
     4381 aaaaagataa ccgagcaaaa agcagagcag caaaactgga tagcaaaaat tggaatgggc
     4441 gcgggaaaag tagttgctac attcaatcaa tcagcaagag aagcagtaga tacgatgatc
     4501 cggacagtga tcccttttat ggctttcgtt tcccttttga ttggtgtgat acaaggttct
     4561 gggatcggta atgtatttgc taacattatg gcacctttag caggaaatgc agttggatta
     4621 acaatcattg gctttatttg ttcactgcca tttttatctc ctttgcttgg tccaggggca
     4681 gttattagcc aagtgattgg aacattgatc ggtgtagaga ttgggaaagg aaatattcct
     4741 ccccaattag cattaccggc actatttgct atcaatactc aaaatgcatg tgattttatt
     4801 ccagtcggtc taggtttgga agaagcaaaa gctgagacag tagaagtagg tgtaccatca
     4861 gttctttatt caagatttct taacggcatc ccacgagtta tcattgcttg ggtagcaagt
     4921 tttggattat atagctgagt
//
LOCUS       DACEOE010000165         4923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEOE010000165 DACEOE010000000
VERSION     DACEOE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.168"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IW774_002334"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IW774_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1154662.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="IW774_002335"
     CDS             complement(2064..3062)
                     /locus_tag="IW774_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1154663.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="IW774_002336"
     CDS             complement(3288..3941)
                     /locus_tag="IW774_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1154664.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IW774_002337"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IW774_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1154665.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1460 a         1024 c          756 g         1683 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt aatatacagt aaaaccagca ttctcgcttt ttttatcatt
     4861 taaaaattag agttttcaaa tctaattcaa attttctaaa catatgaaac agcgtataaa
     4921 act
//
LOCUS       DACEOE010000166         4912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEOE010000166 DACEOE010000000
VERSION     DACEOE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.169"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(400..717)
                     /locus_tag="IW774_002338"
     CDS             complement(400..717)
                     /locus_tag="IW774_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1154666.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(718..972)
                     /locus_tag="IW774_002339"
     CDS             complement(718..972)
                     /locus_tag="IW774_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1154667.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1331..1735
                     /locus_tag="IW774_002340"
     CDS             1331..1735
                     /locus_tag="IW774_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1154668.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1752..2900
                     /gene="tnpB"
                     /locus_tag="IW774_002341"
     CDS             1752..2900
                     /gene="tnpB"
                     /locus_tag="IW774_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1154669.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(3123..3341)
                     /locus_tag="IW774_002342"
                     /pseudo
     CDS             complement(3123..3341)
                     /locus_tag="IW774_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3607..3801)
                     /locus_tag="IW774_002343"
     CDS             complement(3607..3801)
                     /locus_tag="IW774_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154670.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT         1681 a          800 c          764 g         1667 t
ORIGIN      
        1 catagatata ttggacttcc tcttttaaaa cacaaaaata aatactacct tatttaggta
       61 gtatttatta aaagttacgt caattttgat taaaaatata ctacttgaac ctctcatgag
      121 taaagtcacg aaatttttgg gaatagagcg tacttgatat cttatttttt cactaatagc
      181 agtgtctctt attcaccaag cgatccccgt agttcctacg gttcttaatt tttctctaga
      241 ctaatgattg tatctttaga ccttcggcca taacatggag caaaataaaa aggtactaag
      301 tcttatatat caatcttcga gtaaccgacg gttaacaacc atatcgagcg gcattaatca
      361 cccacctgta gaggatgagc gactttttcc taattattgt taaaagccta gtttcatttg
      421 tttagattgg aaaatacgtc ctacaagtat ctcttttttt tcatggtcga tccgataaaa
      481 taaggcatag cttttgccga tcacgatccg atatgttggg atatccattc catagatttc
      541 ggaaacttcc tcatgcatag ttggaaaatt tttggttagt tctaaagcct tctggatcgc
      601 acctacaaat ctttttatgc tggctggaga aagagcaagc tcattttccc attcttctat
      661 tatcgtttca aggctggtct gaaaactttt agaataaact agcgtgtact ctttcatcta
      721 ttcactccag ccgtattttc cgaaagcttt taccatttca atttctgaaa ctgttaaacc
      781 tttctcaaag tcttggaaac tttcttcaat caatttgcga tcgtgttctt ttgctatttg
      841 agtagtatct aatatgaata agttgtcttc ttgtttggct tggataatgt ctccgctttt
      901 taatccaaga gagcttggaa ggatcactcc cacgctattg ccgatctttc taacctttag
      961 ttcttttccc atgttatcac actcctttta gtttttgatg aataaattat aacatgttat
     1021 aacaacaatt gccaacggtg tttcttaagg gattccactc catattatta ttggacaaaa
     1081 taagccactt agaacgctga aaacatatac aaacgatatc aaaatacgag tgaactactc
     1141 accatacact tccgttaaca tccatttaac agaattaggc agaagtcgcc catcctctat
     1201 aggtgggtga tgaatgccgt tcggtatgag ggttaccgtt ggtaactcga agaccgacaa
     1261 ctaaggcgta ttgccttttt attttgtccc atgttacaat cagtctatac aagaaatgag
     1321 ttgatataac atggaattag atacaaataa tcattcagta tttcttcttt attaccacct
     1381 tgttttagta acgaaatatc gtagacaagt gattgatgat gaaatatctg attatgccaa
     1441 aactactttt gaaagaattt cagaatcgta tcacattact ttagtggagt ggaatcatga
     1501 taaagaccac gttcatatta tgttcaaagc tcaacctaaa acagaattga caaaattcat
     1561 caatgcctat aaaagtgcca gctcaagatt gataaaacgt gactttccaa gagtcaaaca
     1621 atttctttgg aaagaaatgt tctggtctaa aagcttttgt cttctgacaa ctggtggcgc
     1681 accaattgat gtgattaaga agtatatcca aaatcaagga aacaatcata aatagaaagt
     1741 aggtgaactt tatggaacaa ctaaaagcat acaaattcag aatctatcca acagaggaac
     1801 aagaaatctt ctttgccaaa tcttttggtt gtgtccgtaa ggtctacaat ctaatgcttg
     1861 atgaccgaaa aaaggcgtat gaagaagtta aaaatgattc ttctaaaaaa atgacttttc
     1921 cgacaccagc taaatacaag aaagaatttc cgtttttgaa agaaattgat agccttgctt
     1981 tagccaatgc ccaacttaat ttagataaag catacaagaa tttttttcgg gataaatccg
     2041 ttggatttcc acgtttcaaa agtaagaaaa atcctgttca gagttataca acgaataatc
     2101 aaaatggtac aattgctttg attgatagta aattcatcaa agttcctaaa ttgaagtcat
     2161 tagtaagaat taagcttcat agacaaccaa aagggattat caaatctgct acaatatcac
     2221 gtcattctag cggtaaatac tatatttctt tgttgtgtaa agaagaaatt atcgaattac
     2281 ctaaaactaa ttccacaatt ggtattgact taggtattat ggactttgcg attctttctg
     2341 atggacaaaa aattgataat aataaattca cgtccaaaat ggaaaagaaa ctaaagcgtg
     2401 aacaacgcaa attgtctagg cgtgctttat tggctaaaaa taaaggtacc aatctatttg
     2461 aagctaaaaa ttatcaaaaa caaaaacgca aagtagctag attgcatgaa aaagtaatga
     2521 atcaacgtac tgattttctg aataagttga gtacagaaat gatcaaaaat cacgatatta
     2581 tctgtattga agacttaaac acaaaaggta tgttgcgaaa tcataaatta gctaaaagta
     2641 tttctgatgt ttcttggtca aagtttgtga ctaaattaca atacaaggct gactggtatg
     2701 gacgaaaaat catcaaagta gataaatggt ttccgtctag tcagatttgt tcagagtgcg
     2761 gacataaaga tggcaagaaa tctcttgaaa ttcgagaatg gacttgtcct gtttgtcata
     2821 ctcatcatga tcgtgatatc aatgctagta tcaatattct gaccgaaggt ctaagactac
     2881 attctatggg attggcttag atccaatcat caagaaccgt aggaactacg gggatagctt
     2941 ggtaaataag agacaccgct gttagtaaag aaatacgcta tcaagtatgc tctattccca
     3001 agaagctccc acttcaagcg ttaagaaccg tcaggtttag ctaagtggtg agtagttcac
     3061 gtaggtattt ttattatata tagatttaac atatgtcggt cggttgattg atatagctaa
     3121 agttataact caaccgaagc cgattcatct acacgaataa agttgcgagt gttctcggct
     3181 aattcataaa aattgttatg tgttccatat agcaatactg gaataacttt attttcttga
     3241 atctcataag caactctata cacggtgacc gcatacttaa atccattaga ccaattacct
     3301 gataaacctc cagttttttg ctcttactta aatggatcaa tggcaatttc atcacaaacc
     3361 tatagaggat gagtgacttc tgcctaatta cgttaaaaaa tattgaaagt aactctatgt
     3421 agcaaaaaat atctagaatt tcatttcctc aaaactgatc gatattgtat ctaaaaatat
     3481 atactaccta aaataggtag tatatattaa aatatgtgct tttgttagaa aaatcaatac
     3541 ataaattatc atatctgtat atgtcattta ggtatttcag ttatgttata atagttttgc
     3601 tgtctgctac cccggcctaa tctggcaagg ggaggaggag atataatgaa ataccttttt
     3661 gattttgttt ttgcaatcaa gccacttgca gatgtagtgt gcagttacct ctgcaaatgg
     3721 cttgattgca aaattgccaa aaaagacagt aagcctaccc gttaattcta taatatagaa
     3781 acgtaaaaaa aaccccatca gcctgcacgc tgatggggtt tggtgatata atgaaatacc
     3841 tctttgttac tcataattgt accataatta gtcacgaatg caaccaatag aaaaaactac
     3901 ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat tttgtgaagc
     3961 attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc ttctattata
     4021 aaagaagata tatatcattt cttaattacc tactacctta aaccaaaatt taagtactac
     4081 ctaatttaag tattacttaa acaatctaaa aaatagttat cccaattgtt atgcttttat
     4141 aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat atttaggtag
     4201 tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc aactaagtta
     4261 tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact acaagcatgt
     4321 agttttatct gttaattatg tactacctaa ataaagtagt ttataattag caagtatata
     4381 tatttaaaaa tttaagatta atttttttat gacttgtaca aatttatctg atattctctt
     4441 attaagaact acctaatttg ggtagttctt attttttaca ttttaagtag tatttaattg
     4501 caatttggta gtattttttt attatcttat tcttagaacg gtagtactta atataaatat
     4561 aggtagtatc taatttaagt attaatggta gtatctaaat taacaggtag tatgtaacta
     4621 ctttaaagta atttttttca gattaacaca gtagtatctt attaaataag ttaattttaa
     4681 tggtagtaca taattattat atatatctga cattggtagt acttattgta ttttttagta
     4741 gtatataatc tattgttttc tagcctaaag ccttgtctta taagaataat ataatatata
     4801 ctactgaaat aaggtagtat atattactag taatggtagt atgttttata tgcataaggt
     4861 agttcttaat ttattttttg ttttgacact ggtagtactt aattatcttt aa
//
LOCUS       DACEOE010000167         4880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEOE010000167 DACEOE010000000
VERSION     DACEOE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.171"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..759)
                     /gene="argS"
                     /locus_tag="IW774_002344"
     CDS             complement(<1..759)
                     /gene="argS"
                     /locus_tag="IW774_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1154671.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDS"
     gene            complement(1179..2126)
                     /gene="arcC"
                     /locus_tag="IW774_002345"
     CDS             complement(1179..2126)
                     /gene="arcC"
                     /locus_tag="IW774_002345"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1154672.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(2241..3260)
                     /gene="argF"
                     /locus_tag="IW774_002346"
     CDS             complement(2241..3260)
                     /gene="argF"
                     /locus_tag="IW774_002346"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1154673.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(3351..4580)
                     /gene="arcA"
                     /locus_tag="IW774_002347"
     CDS             complement(3351..4580)
                     /gene="arcA"
                     /locus_tag="IW774_002347"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1154674.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         1320 a         1019 c          843 g         1698 t
ORIGIN      
        1 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
       61 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
      121 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
      181 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
      241 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
      301 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
      361 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
      421 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta cggtttgcaa
      481 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
      541 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
      601 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
      661 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
      721 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
      781 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
      841 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
      901 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
      961 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     1021 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     1081 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     1141 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     1201 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     1261 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     1321 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     1381 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     1441 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     1501 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     1561 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     1621 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     1681 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     1741 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     1801 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     1861 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     1921 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     1981 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     2041 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     2101 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     2161 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     2221 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     2281 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     2341 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     2401 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
     2461 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
     2521 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
     2581 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
     2641 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
     2701 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
     2761 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
     2821 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
     2881 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
     2941 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
     3001 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
     3061 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
     3121 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
     3181 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
     3241 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
     3301 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
     3361 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
     3421 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
     3481 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
     3541 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
     3601 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
     3661 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
     3721 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
     3781 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
     3841 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
     3901 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
     3961 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
     4021 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
     4081 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
     4141 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
     4201 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
     4261 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
     4321 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
     4381 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
     4441 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
     4501 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
     4561 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
     4621 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
     4681 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
     4741 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
     4801 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
     4861 tatacacaaa tcattcgctt
//
LOCUS       DACEOE010000168         4808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEOE010000168 DACEOE010000000
VERSION     DACEOE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.172"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(90..1175)
                     /gene="xerS"
                     /locus_tag="IW774_002348"
     CDS             complement(90..1175)
                     /gene="xerS"
                     /locus_tag="IW774_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1154675.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3251..3769)
                     /locus_tag="IW774_002349"
     CDS             complement(3251..3769)
                     /locus_tag="IW774_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1154676.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1580 a          786 c          730 g         1712 t
ORIGIN      
        1 ataaaaatga taatgaagct tgcctttaaa gttgggcttt tatctaattt tagagacagg
       61 cttcattatt atactatttt ttactacatt tacaaagcgt tcaaagcatc aattgtagct
      121 gcattgtcta tatgagtata taaagttgtt gtatttgtac ttgtatgtcc caattgttgg
      181 gcagttaata ccaatgaatt tgtctgttga tagagtctag tagccaaagt atgacgtaat
      241 ttatgtggac tgacttggac tctgaatcct tttgaatatt ttgctaccat ccgttcgatt
      301 gcaataccgg atatacgctt ggcttctcct tgataaattg ctagaaataa agctttttcg
      361 ttttctgtta ctttgtatcg ttctttccga atagctagat aattagctaa atattccatt
      421 gcaaatgctg ctatatttac tgcgtctctc ttatttcctt ttctagttac taccacatta
      481 ttcttttcta aagataggtc ctctaaatta atatttacaa cctcggagag tcttaatcca
      541 gatcctaaaa tcaatgcatt gatcgccagg tctctttcta catctcgttg ataatagatc
      601 aaagctcgtt tgctcaaagt ttttttatat tcattggcaa tataatctag ataattttgt
      661 gtatcttcgt ctagaaataa ttttttctcg attgcgtttg cacgggcagc taacgtagtt
      721 ttatctgtga tagttgctac ttgatccata ggattatttt ctaagtatgg ttttcctaaa
      781 ggattattgg aacttttcga aagatattta aatagaacct ttaatgctgt gatatttcta
      841 ttgatagtag aagtagatag acctaagtta ttattatctt tgagcttagt ttttgttgtc
      901 tcttttttta cattttcctt tgtgcgacgt ttcaaatgag atttgtacaa gtttagatca
      961 taggcagtta actgtcttat cgaggtgatt gaagtatcgg gaacgataga ctcactcaga
     1021 agccattcat aaaagagctt ataatttttt agatattcta aagtagttcg aggtgattga
     1081 tctttatcct cacgatcttg aacaaaatct tttatataat cgggcatatg aagtatgatt
     1141 gtatctattt tttgatatat ttctgtattt ttcatgttat tcatcctttc gataagtagg
     1201 ggagaggagt atctctataa ttggactata ccaattatag agtgttttgt caattggtat
     1261 actctgaaat ttattaattt atttcaaaaa agcagataaa taggtataga tttaataatt
     1321 taaaataaat ggtatacatg aataggaaag gaaacagaag gaggcaatct taatttttta
     1381 aattgtctaa attatataga aagaacaatc tttctatata attagacata agagagacta
     1441 cggttgattt gatattaaca agcgtatatg aatgtatata caaataaaaa aggggaggga
     1501 gaagttaaag aaaattcaga gaagtggggc actcagtagt attaacaatt taaaaggaat
     1561 ttcttaaaga aaatgggttg catcctggag atacaatttt tttagataaa gaaaaaattc
     1621 aagcaacaat tcaaaaaaat cttaactgct gagattgatg tagcaatatc tcaatcatca
     1681 acaaagtacg atcctgcctt tcaaaagctt attgataaat gtttattcat tataaaaata
     1741 aaagaaaaat ttatgtcagc ttttttcatc aaagtggtaa tgataattcg tctattaatt
     1801 gtatttttta ggaaatatgg tataattgtt ttaacgaaaa ggtaattcat gatacaccaa
     1861 aaaccactag ctggcgggct agtggttttc ttttacgttc tattcagatt aacgggtagg
     1921 cttactgtct tttttggcaa taattttgca atcaagccat ttacagatgt aatgcgcgga
     1981 tacttctgct aagattgcaa aaaacatatc gaaaaggtaa ttcataatac aacctcctcc
     2041 ccttgccata ataggccggg gtagcagaca gcataaatat tataccataa ctgaaaattt
     2101 cattttagta catttcaata tattgatgaa ccagcacaat aaaacatgcc atgactaaca
     2161 gaaaaaaact tattaaacca gatataactt gtaaacatcc aactatatag aaaacgtaaa
     2221 ttttctatat agttggatta agataacgac tatttatttt atcatatttt tactataccc
     2281 tatactttta cttggtatat ttatgaataa tcgcaaattt acccctatac tactagtttg
     2341 taagttagat taataaaaat actaaagtta tttgttcttt tattaaatac acctacaact
     2401 tataaattga tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt
     2461 tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac
     2521 ttatgaaatg aaagaccttt tacatcttgc agaaatcggt actgggatta aactaagcaa
     2581 taaaaactaa tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata
     2641 aatgacgatt tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc
     2701 agtatcagta ataggatagg cgataggcca catacccttt gatggttaga ataaactgct
     2761 caaaatctgt atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa
     2821 ccataagcgt tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata
     2881 taattaccgg acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa
     2941 ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct
     3001 agtctcctaa gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt
     3061 catggtttat ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca
     3121 ttctagggga gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg
     3181 tagtcgtatg ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt
     3241 aaacgggaat ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa
     3301 atggagcaac tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg
     3361 cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa
     3421 tcatcatgtt aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta
     3481 gatgtgcata ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt
     3541 gatcagaagg cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta
     3601 aattcgtcca accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa
     3661 ctcttttatc cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag
     3721 tcctatttat ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc
     3781 cataaattcc taattgtctc ctttaattca tctataattt catccacgat attttaatcc
     3841 gttctacaat gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa
     3901 aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga
     3961 atatatttct tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca
     4021 aaatcatgtt aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg
     4081 ttaagtttag ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt
     4141 aaaactcaaa atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg
     4201 taaaaaatat tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa
     4261 acagctacaa aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc
     4321 atattcacgc ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt
     4381 ttaggaaatt gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc
     4441 accagatatt cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca
     4501 ccgcgaattt actaaaatta tgaataatta tgttaatctt ccgaaatttt tttccaaaat
     4561 taagggatca agctggtgtt atagattgtt taaggttggc aaaaaaataa cttcttttta
     4621 aacaattata gaaaaccgat actctgttaa gttggttgac acggtgtcga tttttatgac
     4681 tttcttattc tgaaacttca ctaacggaca gtacctaaaa agtctatagt atggacgttt
     4741 acgagtacta ttttatcaaa aatgctgctc atttcaactt atatgtttta gtgaactacc
     4801 cccactta
//
LOCUS       DACEOE010000169         4754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEOE010000169 DACEOE010000000
VERSION     DACEOE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.173"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(235..1458)
                     /locus_tag="IW774_002350"
     CDS             complement(235..1458)
                     /locus_tag="IW774_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1154677.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(1489..2328)
                     /locus_tag="IW774_002351"
     CDS             complement(1489..2328)
                     /locus_tag="IW774_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1154678.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(2345..3025)
                     /locus_tag="IW774_002352"
     CDS             complement(2345..3025)
                     /locus_tag="IW774_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1154679.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(3027..4100)
                     /locus_tag="IW774_002353"
     CDS             complement(3027..4100)
                     /locus_tag="IW774_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1154680.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
BASE COUNT         1314 a         1045 c          805 g         1590 t
ORIGIN      
        1 tcactttcaa acttttattc aaatgtatgg actttccatg taattcgtca attaggcttg
       61 atcggttcac agcgaccacc gactttctga acaaaacacc taaccgctac tgcgtgaaaa
      121 gttgacttgt acgaagttta ccttccataa gatcatttgt caatgaaaaa gaaaatattt
      181 tttcattttt ctgaaaatta tttttataac tttcacacac aagtttattt tttactagaa
      241 agtagcgccc tcttgttttt ttcttgctac tatttcttga tgttcttgct tgattctttc
      301 taatagcttt ggctctttca acagatcaag agctgtattg gcgagtagtt ctgctgcaat
      361 cgcaatagaa gcaaggcctt tgtcactttt agcagcagct ttgaattctt ctgtgtgtcc
      421 ggcaatatat gtctcagaga ttgctaccgt aggctggatt gttggtatga cttggctgac
      481 atttcctaca tcagaagaac cgagacttgt tctttgttcc gtttctattt cttcatctgg
      541 aacacctgct tcttttgcat gctggaagaa taagtcatca aaagaaggag taacgatgac
      601 atcatcaact gcattttgga acaatccaaa ctcaaaagtc gtgcccgtac ttatcgcagc
      661 acctcgtacg atattttcca ctttttcata tactttatct aatgtttgcc gatttcctgc
      721 acgtaaatag aaacgtccag cagcgtaatc tggaatgacg ttggcagcta ctcctccgtc
      781 tgtaatgatc ccatggatat tgacatcttt tggcaattgc aagcgtagag cgttgattcc
      841 attaaacact tggatcaatg cttccaaagc atttactcct ttttcaggtg ctgctgctgc
      901 atgggaagcg acaccgtaaa atttgatgtc gactggatca ttcgctaaag attctgtcgt
      961 tttcccataa cgaaaagctg gatggacgca taacgctaca tcgacatcct caaaaaaacc
     1021 ttctcgaaca aagctgcctt tagctgatcc gttctcgccc ccttcttccc ctggggtgcc
     1081 atatacacgg atttctccac cagtttcatc aatgaattgc ttcactatgc tggcggctaa
     1141 tacggagtaa gtaccaaaca aattatgccc gcatgcatga ccaatacctg gtaaagcatc
     1201 gaattctgcc aaaaaggcaa ttgtgggacc gggtttattg gaaatataac gtgcatcaaa
     1261 ggctgttcgg tgtccagcta cttctttttc tacccaaaac ctttcttttt ctaattgttt
     1321 aatcaacgct gctgaggaat aaaactcata gttacttact tcaggatgtt cgtggatatc
     1381 tagagcaagt gcttgatatt ctggtaaacg atattggatt agatccttca attggtttgt
     1441 tgagattgtt gttgtcataa aaaattcctc ctatgctagt catttttctt attttctccc
     1501 gaatgtttcc catgcaggca cgcttgatcc ttttgatgtt ttctcgatta ctttgactgt
     1561 gtcttctgtt tgataagcgt ctactatttt ttgatacgtt ttattgtttt catcttcttt
     1621 tcttgcaacg atgatattca cataaggacg tgcatggtca tccactggtt ctaagaagat
     1681 ggcatcagaa gcaggattaa atccagcatc tacagctaca ccgctattga tcactgaaat
     1741 gtctacatct gacaaagcac gtgctgtttg ggaagcatct aattcagaaa tgtctagatt
     1801 ttttctattc tcggtaatat cagaaacagt gggcgcttgt ccttttgcct tatccacttt
     1861 gatcaatcca gcagtttgca ataacagaag agctctgccg ccatttgtaa catcattagg
     1921 gattgctact tttgctccgt ctttgatatc agctacatcc ttcaaagtgt tagaataaat
     1981 tcccagcggt gcattgacag tatttccgat cgatactaaa tcagttccgt gttctttatt
     2041 gtaattgtct aaaaagaatt gatgttggaa agaattcaaa tcaatcgatc catctgctaa
     2101 agccacattt ggttgattgt aatctgtaaa tttcacgtat tccaaattaa tgttctcctt
     2161 tttcaaacgg tctttcacgt tgtcccatac atctgtatct tctccaataa tccctagttt
     2221 tacgatagtt tcttttttgt cagttgtatc tgcttttcct tggctacacc ctgccaacca
     2281 aataattgct acaaatgtaa tgactacaaa acgaataatt tttttcattt tttctcctcc
     2341 actttcaatg tgtaattttt ttaatcagtt gattactgat aaattgactt aaaaaaacta
     2401 agattagaat caataatgta gccacaatcg ttacatctgt ttggaatcga ttataccctc
     2461 tcgatatagc aagattccct aatccgcctg cacctactgc tccagccatg gtagtcagtc
     2521 cgatcacgtt gatgatagtc aacgccgaga cccgaatgat accaggcaaa ccttctaaca
     2581 aatacacacg taaaatgata ccgattggac tggttcccat cgcttctgcc gcttcgatca
     2641 ccccaggatc aacttctagc agtgcgttct cgatttgacg agcaaaaaac ggaacaactc
     2701 caaaaatcaa tggcactaaa gctgctttcg caccaatcgt cgttccaact aagaaacgag
     2761 tagtcaatgc taaaagagaa agcaagataa tgaaagggat ggaacgaatg atattgatca
     2821 gcttatcaag tatttcaaat agcagacggt tagctagtaa tccattaggg cgtgtcacta
     2881 ctaacgtcac acctaagatg attccaatca tcccgccaat gaatgctgtc caaaaagaca
     2941 tatatagtgt ttgcttcgtc gcttcgacaa attcatccgg aatcaaataa acattgggca
     3001 aatatgtttt aaagaaatca gtcattttat cttcctcctt cgatcacttt gatacgacta
     3061 tctaaatcgt ttttttgtaa aatatttatt tttacaccag atttgatcaa ataatccaat
     3121 gcttttttcc gctgtttggt ttctccagct aaagtaacga tcaaactccc taacggcgtt
     3181 tcttgtaata actctacatt gccatataaa atattcgctg ttacttgata tttgctgtac
     3241 agatgagcga ttagaggttc gttcgtctga tttccaatat aacttaattc caccagccat
     3301 ttattggcga tctgatcggc aaaagcagaa tgggcaatga tcgtttctaa tgcttggtca
     3361 agatgagtag ctgttcgaat aaaatctttg gtcaattctt cttcaggatt gctgaagatc
     3421 tggatgctgc tacctttttc aacgattctt ccatcttcca taaccgctac cttgttgcag
     3481 atttctttga caacttgcat ctcatgagtg atcaggacga ttgttaaacc taactggcga
     3541 ttcaattttt ttaacaatgc aaggatctga agggtcgttt tcggatcaag tgcactggtt
     3601 gcttcatcac ataataatac cttaggttca ttcgccaaag ctcgtgcgat tgctactcgt
     3661 tgtttttgtc cccccgacag ttgtttagga taagcagatg ctttttcttc caaaccgaca
     3721 agttccagta gttcattcac tttttgacga cgttcctgtt tcgattttcc agaatacttt
     3781 aaagaaaaat caacgttgtc aaaaatagtt cggctttcca ttagattgaa atgctgaaag
     3841 atcataccga tggcctttct ttcttttcgc aattctttgg gagagagctg actcagattc
     3901 gtctgattga caatgacttc tcctgctgtt ggtttctgca gcaaattcat cacacgaact
     3961 aacgtacttt tccctgcacc tgagtaaccg acgattccat aaacatcgcc tttttcaatc
     4021 gttaaatcta cagaatctac tgcctgtatc tctttttctt tttgtttgaa acatacagaa
     4081 acatctcgca attcaatcat taaaattcct ccttatctaa aaaaagcgtc ctcaagaaaa
     4141 tgaaaatgtc attttcttga ggacgctttt ttgcgtgtta ccacctcatg ttcaagctgt
     4201 gttcacacac aactcctctt caagtacgat catactctag caagatatcg gtcgcagccg
     4261 tcatcgcctt acatctatgc tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt
     4321 ccttgcttct tctcagctac cgaagctctc tgtcaaaggt tcttctatcg tacttaccat
     4381 ttcaatgcaa ataatattca aaaaaaaagt ccttttacta acataaacta tgtcagtaaa
     4441 aggacgacgt atcgcggtac cacctttttt cggaagtttc cttccctcaa gcccaacatc
     4501 tcaaaaagag agattggcgt tcgttttaac ggtcgaacag ttccgaattt gcctacttat
     4561 cgacaaatta ttctccaagg ccattttcag cttttcttac ttgactcttc tcaccagcca
     4621 gagcctctct gcaaagtttc caaacttact ttccttttca acgaaatcga tattttgttg
     4681 ttgggattaa tctacatgag gaaaactgaa aagtcaagta cgaaattcaa attatttttt
     4741 ctatatttat tttt
//
LOCUS       DACEOE010000170         4706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEOE010000170 DACEOE010000000
VERSION     DACEOE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.175"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..1055)
                     /locus_tag="IW774_002354"
     CDS             complement(93..1055)
                     /locus_tag="IW774_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1154681.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1057..2496)
                     /locus_tag="IW774_002355"
     CDS             complement(1057..2496)
                     /locus_tag="IW774_002355"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1154682.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2681..4627
                     /locus_tag="IW774_002356"
     CDS             2681..4627
                     /locus_tag="IW774_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1154683.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1499 a          802 c          772 g         1633 t
ORIGIN      
        1 gttgacaact cctttttgtt tacaaatcaa ttggatatct acaaattgta ttttctaacg
       61 aattttatag acattaaaaa attaatttaa cgttaaatac ttcctccaga aagtattgag
      121 attggtaaaa tataatcctt atttgtcttt tcgcctttaa tttttttcag aagaatatca
      181 acaataagac ttgcaatttc atcaattggt tgtcttattg ttgtcaattg tgggaaaaat
      241 ttctcaataa aagaggtacc atcgtatcca ataattttta aatcttctgg gatattaagt
      301 ttaagttgat gagcaatttt catagtgaga atggctgtta aatcatcaga aacaaaaata
      361 ccatctggtt tgtgatttat aatagttgac tttatttcca tttcacgacg gatagtagac
      421 agattattag gaattttgaa aatttttcca tttggatttt gaaaagaaaa acctagtgca
      481 cgtaattctg tgggagaatc cgtgttatca tttcctgtaa tcataatggt attttgacaa
      541 ccattttttt gtaaagtctt agctgcaagt tttccccctt caaagttatc tgaagaaaca
      601 atggaaacat taggagctaa atttctatca aaagcaacaa taggtgctct cacacgctca
      661 taatcagcaa ttccaagatt atggcttgat gagattatgc catcaacttg gttggcttca
      721 agcatttcaa tgtattctct ctctttagca ggatcatttt gactattgca aataatcgtt
      781 ttatatccat gtttaaataa ctctatttcg agatactcaa tgagttcaga gtaaaagata
      841 ttactaatat taggaaaaat aagaccaata agctgaggtg actttccttg aagtccacgt
      901 gctaaattat ttggtttata accaagagtt ttcatagctt catgaacttt ttgttttgtt
      961 ttttctgata aatatccctt attattaatg acgcgtgaaa cggttgttgg gctaactcca
     1021 gctaattcag cgacatcagt taattttgct accattttaa tattttaatt caaagtattt
     1081 tccagtaggt tcgcctgatt tgatttgaat accattttgg tgattttcag ggaatacacg
     1141 actagtaaac actttttctc ctttattaat aaaaatctca atgattgagt tatctacaaa
     1201 aatgttagca gttgtttctt tagtatcaat tgagcattca cgaatcgtac caaagtcagt
     1261 ggcatattga atgcctgctt tgctgcgatc taaaataatt tttccttctt tggtatctac
     1321 aatcaagcta agtccatttc ctttactatc agagaaaaga agtaactccg atttctgatt
     1381 tggaggaaaa gttagttcaa gctcataagt attatttgtt tctgcttttg ctgtaaattg
     1441 ttcagaacta gcacgtaatg atgtaatagc ttctactgga tattggtaaa gtttaccatc
     1501 ttttattgaa agttctttta caagactcat tgctccctga taatcatact tatcagttgg
     1561 ataatcgata tcaggtagtc caatccaact gacaatcaga gcacgaccat caggagtatt
     1621 gaatccttga gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc
     1681 cacaagtttt actttttcta tgtcaagcga ttgacagatc ttaaaagtat tcggatagat
     1741 attcccataa tttagctcag atttatccaa cccttgagga caataaatga gaacaggttt
     1801 tttgtctaca aatactagat ttggacactc aatcatgtat tctgaaccag tgccgccaaa
     1861 atcaaggttg ccaacttcct cccatgtttc cacgttattg tcatccgctt tgtaaagttt
     1921 gataaagcct gatttttcta aattttgtgc tccaacaatc gcatacaatt gtccttcata
     1981 attaaacatt tgtggatcac gaaaatgatc agttacatca gcaggtttat gaataagaac
     2041 attctcacct ttcgtaatgt tgtagtcttt atccatccat gctccgattt gaagtgtatc
     2101 acgttcccaa ttttcatttc gaacgtttcc tgtatagagt aagaatagat tcccctctat
     2161 ttcatatgca gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc
     2221 tgctcctgtt acatgaaaat gaactaaatc atctgattca ttgtgtaccc attctttcaa
     2281 cccatgagca gctccaaatg gccaattttg ataaaaaagc gtatattttc cgttaaaaaa
     2341 agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat agctagcacg
     2401 ccaaggtgat cgttttacat tatccttaat tttatccatc tcatctttag tccaatcaga
     2461 ataagcacga tatcgtattt ctcgaggtaa gttcatattt tactccttca atttatcttt
     2521 ttacaactag tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata
     2581 aactatatga caaacgtttg acatattttt aagatatgct aaaataaatt tgcatttcag
     2641 tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga atgaattaca gtaaagtagc
     2701 ttccgacgta atacaagcgg tcggaaaaga taaccttatt gcagctgcac actgcgctac
     2761 acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat caaaaagctt tagacgaaaa
     2821 tccagatgtg aaaagcacat ttaaaattga tggacaatat caagtgatta tcggtgcagg
     2881 tgatgttaat ttcgtttatg acgaacttat caaaaaaact ggactttcgg aaatttcaac
     2941 agatgattta aaacaaattg ctagtaaaga taaaaaattc aaccccatta tggcattaat
     3001 taaattatta tcagatattt ttgttccaat tatacctgcc cttgttgcag gtggtttatt
     3061 aatggcatta caaaactttt taacagctgc tggacttttt ggaccaaaat caattgaaga
     3121 aatgtatcct gcgattgaag gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt
     3181 catgttctta ccaattttag taggtatttc agctgctaaa cgttttggag caaaccaatt
     3241 tcttggtgct gcaattggta tgatcatgac aacaccagat cttggtggct caactgaata
     3301 ttggaatatt tttggttatc atgtttcaca aacaaactat gcttatcaag ttattccagt
     3361 tttagcagct gtttggatat tatctatatt agaaaaattc tttcataaaa aattgccatc
     3421 atctattgac tttacattca cgccattatt atcagtaatg attactggat ttttgacttt
     3481 cacagttatt ggccctgtaa tgttattgtt gtctaatgga attacagaca ttattgtttg
     3541 gctatataac actactggat ttattggaat gggtattttt ggaggaacat attcacttat
     3601 agtcatgact ggtttacatc aatcatttcc agctattgaa actcaattac tttcagcgtg
     3661 gacaaatggt atcggacatg gagactttat ctttgtagtt gcatctatgg caaacgttgc
     3721 tcaaggtgct gcaacatttg ccatttggtt cttaactaaa aattctaaga caaaaagtct
     3781 tgcttcctct gcgggtgtct cagcacttct tggaattaca gagcctgcct tgttcggagt
     3841 taacttaaaa taccgctttc cattcttttg tgctttaatt ggttcaggaa ttgctacagc
     3901 agtagcagga ttacttaaag tagttgctgt ttcacttggt tcagcaggtt tcttgggatt
     3961 tctttcaatt aatgcaacat ctattccatt ttacgtattg tgtgaattga ttagctttgt
     4021 agttgctttt gcaattactt acttctacgg taaaacaaaa gctgctggta tctttaaagc
     4081 agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa cagatccaat
     4141 atctactgat gttgaatcaa cacaaacaaa agtttcatca gaaacaattg ttagtcccat
     4201 ttctggtaaa gtgattagtc tttcttcagt aaatgatcca gtcttttcat cagaagctat
     4261 gggaaaaggg attgccatta aaccaaatgg aaacacggtc cattctccag tagatggtac
     4321 tattcaactc acgtttgaaa ctggacatgc ttatggtatt aaatcaaata atggtgctga
     4381 agttctaatt catattggta ttgatactgt atcaatggga ggaaaaggat ttacacaaaa
     4441 agttactact aatcaaaaag ttagaaaagg cgatattctt ggagcatttg accgttcaat
     4501 gattgagaaa gaaggtcttg atgatacaac aatgattatt gtgaccaata cttcggatta
     4561 ttcagaagta aatatagtag ctgaaaatac agtcactgag ggtactgatt tacttgaaat
     4621 caaataaata tatttccctg agtaaagatg gaattttctt tccaaaatca aataatatag
     4681 attaatcaaa tatttgagaa taacat
//
LOCUS       DACEOE010000171         4702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEOE010000171 DACEOE010000000
VERSION     DACEOE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.176"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(441..1253)
                     /locus_tag="IW774_002357"
     CDS             complement(441..1253)
                     /locus_tag="IW774_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1154684.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1416..1523
                     /locus_tag="IW774_002358"
     CDS             1416..1523
                     /locus_tag="IW774_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1154685.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1581..2648
                     /locus_tag="IW774_002359"
     CDS             1581..2648
                     /locus_tag="IW774_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1154686.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            2790..2993
                     /locus_tag="IW774_002360"
     CDS             2790..2993
                     /locus_tag="IW774_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1154687.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEQLFHIQVAE"
     gene            3053..4255
                     /locus_tag="IW774_002361"
     CDS             3053..4255
                     /locus_tag="IW774_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1154688.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1562 a          745 c          982 g         1413 t
ORIGIN      
        1 agaatttgag aatgcactgg taatttgtat gacatgtact gatgaagtat ttcgaaaaca
       61 tttttaaagg ttgagatgta tatagattta accaatcatt tatctctctc ggaaaaggaa
      121 tattaactaa ccagcgggtt atcaaaaata ggtaggaaaa ctgtacttaa tggcttattt
      181 cgcgctgatt atgagaatat cagtctgttt gattaatttt tagggttcaa aatacagaga
      241 caaactccag gtcgggtttc gccacaagcg aattacccga tctggagttt tgtctattgc
      301 aggaaggcgt agccggactg caggaataaa gcccccggtt ctaacagggg gctaacaaag
      361 aacaggaaac tgtgacaact acccattggg gagtgtccta gcagtatttt ctttgtcacg
      421 tgcttatcaa ttttgtcacc tcattttgat gatggatacg ctgcatttaa caggcgttct
      481 ttctcgactt ccatcacgaa tggtagataa agatattgct gttctagtag atattgattg
      541 tcctctgtgt actgataaga agcggtttct tctgaataga ccaacggggc aattcgcaca
      601 tcgctggtca attgattaat tgagtataga aggatggctt tctcacctag ttccttgggt
      661 aaatgtttcc caaaatagga ataggatttt tgatttgcta aatagacgag tatgcgatcc
      721 acactaaagt tggagaaggt aaagggcatc atgtgcagaa tatgattttg attatcatgt
      781 tgcaaataga gctgggcttc cttaccaaaa ttagttgaac caagataaag ctgctttttt
      841 tcatcgatac attggaaagt aaactggtct tgttcagttg gtggaatgag atttgtaata
      901 aaaaattcaa tcattcgatc catttctttt tcactaacaa ggtcagcgtg tttgatttcg
      961 tctactatga gaccttcaat cttaggatca agcttacctg cctttttttg gagcatttct
     1021 aaaatgtatt gattctcttg atcaccatac aaaagatatt cattggtgac atgacctatc
     1081 tctgcgattt ggtgaagggt ttcggttttt ggaaggttga tcccacgttc ccagttattg
     1141 actgtacttc gcggtaaatc accaattagt ttgccaaatt tctccatact caactttaat
     1201 tcttggcgaa tctgccgaat tcgattgccg actgcttgct tgtctatctc catgaaaact
     1261 cctcctcagg ataagttctt aaatttagta tacgtgtgta cttaaaaaaa gtcaaaacat
     1321 attgacttaa aataaataca ggcgtacaat aaaaatgtac ttaaaataag ttcaataggt
     1381 tctttgacaa gtaaatagca gaaggaggtt tgtcgatgaa ccaagttaat caacatgatc
     1441 tcggtgaagc cattcgagtt tctcgagaag aaagggggtg gacacaacga tatttagcag
     1501 aaaaagtggg gatttcgaga tagttattat cgaaagtaga aaaaggaacg agacaattaa
     1561 gtgaagaaaa attgaatctt gtgttggata gtttgcaaga agaggtcgtt ccagttaacc
     1621 gtgttttgat tgattatcta accattcact ttttctcgaa tcaacattta aagctaattg
     1681 aagagattat tgaaatgccc attgaacgtt ttgaggaact ggattatgcg ccaaagggct
     1741 atattggcca atacgtgtgg aatcaagtaa tcacgattcg gtattccatt gacgatgcgg
     1801 ttaaaggaac tgtgatggag ttttcaggtc aaggctgtaa acacttagca agatggctaa
     1861 aaacacgaaa aacaaattgg caacagtttt ttcgtaaggt attagattat caggggaact
     1921 ttacacggat tgattttact ttagatgatt ttgtggggag tctcagcatt ccagaactta
     1981 agcataaggt aatactaggc catgtgtgga ccacttttca agtgagtgaa tctcatggcg
     2041 gtacggatat cataaacaat gaatcaaatg gtgagacctt gtatttaggc tcaaagaaga
     2101 gtcagtgtcg tttttgcttt tatcagaagg accatgaaca acggaagcga cgaggaattc
     2161 ctttagaaga ggcaaaagta aagaatcgct ttgaactacg gtatcgaaaa gagaaagcgc
     2221 aaagcttggc aaagattatt tcaaaaaccc atgatttaac caaactgttc tttgagttac
     2281 ttaatggagc aatttgtttt tatgaccgtg atccaaatga tctaggtgca aaagttgatg
     2341 ccaaatgggc agcctttatt agcaattatg gcgcaatcac catttcttta gatacaattc
     2401 ctcaaagttt tgagaaaact atgagttggt taattcatgg tgtttcacca tcattagttt
     2461 ttattgcaca ggtcgatcaa acgtttcatt cttccttgtt gcctttgatt attgaacaag
     2521 gagaaatgaa tccaagacag atcaaacttc tccaaaatat gaaggcagac ccggagtatt
     2581 atcaggagga ggttgagtat tatgaacgaa aattacaaag tatatcaaat gagaaaacaa
     2641 gctactaaaa aagcaaagta ctgcttacac aacaagttgt gcaacgtact ccgcctcgac
     2701 aagacgagta taacacaacc atcgaatgag aaacaataga ttataaagag gattatttta
     2761 aaaggagtaa agaatcagga ggtgagagaa tggaaacaaa atgtgatttc atggtaaatc
     2821 gtgcaatttt gatagagatg ggctttaaac ctagtcaagc tgcacgaatg attaaagaga
     2881 gcaaggcata tcttgcaagg attgaaggta ttgattttta taataatcgc caagttggtg
     2941 tggtgccatc ccgagtaatt gaacagcttt ttcacattca agttgcggaa taaacagtac
     3001 aagcgcggta ctattaaagt agcttttctc aagtacaggg aggaatggaa cgatgccaaa
     3061 aattaaaaat atttaccaag ataaaaagac aaaaaaatgg ttcttccgag cgtatctagg
     3121 tatagatgaa aatggaagaa aggttcagaa gactaaacga ggttatgctt cacaaaaaga
     3181 tgccaaaatt gcttatgaca agtacatgga aacacatgat tttaacaaga caattcctaa
     3241 tcaactgtgt tctaccaatc aaatgacttt cgaagagttt tacaaggata ggtttgtaaa
     3301 atggtacgag agacaggtca aaagtcaaac atatgagaat gcgcaattca ttttcgaaaa
     3361 gcgtatgcag tttttctatc attttcgtgt tcgagatatt acaagtcacg atattgagga
     3421 ttggttattt gaactaagcc agacagagtc acgtaattca cggaaaaaag ttaatggcga
     3481 aacgttatca aaatcttata tcaatcggat tcgagggcac ctaaaaattg tattggatcg
     3541 ggctgttaaa gagggggtga ttgctaaaaa tcccgttgaa gatgtttctt cgcttccttc
     3601 tgagaataag aaagttgagt tttgggaaca cagtgagttt caaaaagtga tgaatgaatt
     3661 gaaggatgat aagattcaaa cccaccatcg gaagattgtg tatgaaatgc tcttttatac
     3721 tggcattcgg attggagaac ttgaagcttt gtcttggtcg aatgtagatt ttgagaaaaa
     3781 cacgataacg attgagaaga cattgattta taatacaaaa gatgattggt acttttcgac
     3841 tcccaaaact aaatgtgctt atcgaacgat tgggttgggg aagaagcttt ccttaaaact
     3901 cagacagtgg tatgacttgc aaaaacgtat tggaaatttt gaatatgttg ctcagttgga
     3961 cggaacattt acgccacctt attcttttgc gaactggtta aaagaagctg ctaaaaaagc
     4021 gggtgtcaaa ccgattaagc ttcacgcgct acgacattct catgttgcat ttctaattga
     4081 gcagaatatt caaccgctat ctattcaaga acgattgggc catgcgaata ttcaaatcac
     4141 tttaggaact tacggtcact tatatgcaaa gtcagatcaa caagtggtaa gtgcgattga
     4201 taatgttcaa gagggcgcta ttctttcaaa aaatgacaat ttattaccag tctgagtttt
     4261 ccacggttct accataagac aaataaatgc ttttgcacca gtgttttcag tcgaattgct
     4321 cctgttcaag atgtatcgga aatagcgaga aaataagcaa atttgcataa ctataataaa
     4381 aaggacttag ttttgatggg aaatttttca aaattaagtc cttttttatg tggttgaaac
     4441 gggtatgatc attttaagga agtgatctaa gatgaaagga cgactgaaca attatcatat
     4501 tagaaaagct ttattgtatt ccacaaacaa attactaaga ataacaaatt agtttttgtt
     4561 tgttcttatt ccctattgac ttttctttca aacatgctat tctaatttta ttctaatagt
     4621 atccgatgag aaggaaagta gacagaaaat ggttcaagag aggctttggt tggtgtaaaa
     4681 agctagaagt tgactgtcga aa
//
LOCUS       DACEOE010000172         4691 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEOE010000172 DACEOE010000000
VERSION     DACEOE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.177"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..280
                     /gene="era"
                     /locus_tag="IW774_002362"
                     /gene_synonym="bex"
                     /gene_synonym="rbaA"
                     /gene_synonym="sdgE"
                     /gene_synonym="yqfH"
     CDS             <1..280
                     /gene="era"
                     /locus_tag="IW774_002362"
                     /gene_synonym="bex"
                     /gene_synonym="rbaA"
                     /gene_synonym="sdgE"
                     /gene_synonym="yqfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Era; Escherichia coli Ras-like protein; Bex;
                     Bacillus Era-complementing segment; essential protein in
                     Escherichia coli that is involved in many cellular
                     processes; GTPase; binds the cell membrane through
                     apparent C-terminal domain; mutants are arrested during
                     the cell cycle; Streptococcus pneumoniae Era binds to RNA
                     and Escherichia coli Era binds 16S rRNA and 30S ribosome;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1154689.1"
                     /translation="PHSVAVVVDSMKRNENDKIHIQATIIVERDSQKGIIIGKGGKML
                     KQVGTKARKDIENLLGDKVFLELWVKVQKDWRDKKVYLQDFGYRKDEY"
     gene            423..1235
                     /gene="recO"
                     /locus_tag="IW774_002363"
     CDS             423..1235
                     /gene="recO"
                     /locus_tag="IW774_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1154690.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            1619..2536
                     /gene="glyQ"
                     /locus_tag="IW774_002364"
     CDS             1619..2536
                     /gene="glyQ"
                     /locus_tag="IW774_002364"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1154691.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            2538..4613
                     /locus_tag="IW774_002365"
     CDS             2538..4613
                     /locus_tag="IW774_002365"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1154692.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         1616 a          852 c         1002 g         1221 t
ORIGIN      
        1 gcctcattct gtcgcagtcg ttgttgattc gatgaagcga aacgagaatg ataaaatcca
       61 tatccaggcc acgatcatcg ttgaaagaga tagccaaaaa ggaatcatta tcggcaaagg
      121 tggaaaaatg ctgaagcaag taggaacgaa agcaagaaag gatatcgaaa atcttttagg
      181 agacaaagta tttctagaac tttgggtcaa agtccaaaaa gactggcgag acaaaaaagt
      241 ctatctgcaa gacttcggct accgaaaaga cgagtactga tattctggac ttcttatagg
      301 ttaggtatag aaataaactg gataaaaatc cgtacgttga gaaaaacagc gaaagatcgt
      361 aacataaata atctggatcg gcagaatctg cttttccaga tttatttttt aggagggaga
      421 atatgagcca gcttgcagaa tcgaaaggct tgattttatc tagccgtaat tacaaagaaa
      481 aagataaatt agtcaaaatc tttacggaat cttctggcaa aatgatgttt tatatcaaag
      541 gaatccatcg gcaaaatcag ccattagctc cggctttgct cccctttacc gaagcagtct
      601 atatcggtaa ttttctaagt gaaggtttgt cgtttttaag cagtgtcaaa gacgtccatg
      661 cgtttcggaa catccaagaa gatatctttt tgagcggtta tggtacgtat ttgctaaatc
      721 tagtagatgc agcaatcgaa gatcatcaat atgacccgca cttattccaa tttacgcaac
      781 aagctctgca gcggatggac caaggagatg atgcagaaat catcacgaat atctttgaag
      841 ttcaaatact gcaacgcttc ggaatcgcgc caatttggac gcactgtgtc gtatgcgggg
      901 aaacaaaagg aaaatttgac tattcttcca aatatggcgg cgtgatatgc gagaaacact
      961 ggccaatgga cgaacaccgt tatcatgcat atcctcgcgc tgtttatttc gttcgaatgt
     1021 tttcggctat ttcttatgat aagatcagtg gaatccactt gaaagaagaa acgaagcagg
     1081 caatccgtga cctcattgac cagttgtatg atgaatatgt cggtattcat ctaaaaagca
     1141 aaaagttcat tgatcaaatg aagtcttggg cacacgtatt aaagatgcca gagaaaaata
     1201 tgcctgaaaa agagatacct gaaaaagaag aataaatgaa agatacaaca aaaaaatcaa
     1261 aactatttga caaatacagg gacttccgtc tattattaac cgtagattaa atagatcgtt
     1321 cactgagagg atctattata taaacaagaa aactgcaaaa tgaatacgta cattgaccaa
     1381 gatcagtaaa aaacacgtgg tttagcgagt tcgggagagt ggaagccgga cccaaacgat
     1441 ttttgaagag gcacttgcga gtacgataaa tgaagctgcc gaatctttat tcggaagtag
     1501 ggtggaaccg cgaaattatt cgtccctatg tctgtaatca gacataggga ctttttgtcg
     1561 ttttctttcc tgtattctta ttgacagcaa cgcttaaaaa ttgaaagggg caaaacagat
     1621 gagtaaaaaa ttgaccgtcc aagatatgat tttaacatta caaaaatttt ggtcatcaaa
     1681 cggctgcatg ctgatgcaag cttatgatac agaaaaagga gcaggtacga tgagcccata
     1741 tacattctta cgggcaatcg gaccggaacc atggaatgct gcttatgtag agccatcccg
     1801 ccgaccagct gacgggcgtt atggagaaaa cccaaaccgt ttgtatcagc atcatcaatt
     1861 tcaagtcgta atgaaacctt cacctgaaaa tatccaagag ctttatttag aaagcttgcg
     1921 ttttttagga attgatccat tagaacacga tattcgtttt gttgaagaca actgggagaa
     1981 cccatcgatg ggttgtgccg gtgtgggttg ggaagtatgg cttgatggga tggagattac
     2041 acaattcacg tacttccagc aagtcggagg actaccttgt aaaccagtta cttctgagat
     2101 tacttatgga ctagaacgtc ttgcttcata tattcaagaa gttgaaagtg tctatgactt
     2161 agaatgggct gatggcgtga aatatggcga gatcttcaaa caaccagaat atgaacattc
     2221 caaatattct tttgaagtaa gcgaccaaga cttgctacta agcaactttg atcgttttga
     2281 aaaagaagct aaacgctgta tcgatgaaaa tcttgttcat ccagcgtatg attatatttt
     2341 gaaatgcagc catacattca atttattaga tgcccgcggt gcagtatccg taacggagcg
     2401 tgcaggttat ctttcccgta tccgcaacat ggctcgttct gtagccaaaa tctttgtggc
     2461 agaacgcgaa aaattaggat tccctttatt gaataaaaac acagcaaatc cagaaaacgt
     2521 gaaggaggct gaataagatg gcaaaagact tattacttga aattgggctg gaagaaatgc
     2581 ctgctcatgt tgtcacacca agcatgaaac aattagaaga aaaaacagca aaatttttag
     2641 atgaaaacca attgacttat gatacgattg aaacattttc tacaccgcgc cgtttagcaa
     2701 tcaaagtaac aaacattcca gaacgtcaag cagacagtga agaagaagtc aaaggaccag
     2761 ccaaaaaaat tgctttggac gcagatggaa actggacgaa agcagcggaa ggatttgtcc
     2821 gcggacaagg tctgacgaca gatgctatca cttttagaga attaaaaggc gtggaatatg
     2881 tctatgtaac gaaatacact cacggcaaag aagcaaaaga agtattgaca ggtttagcag
     2941 atgtcattaa aagcctcact ttcccagtaa cgatgcattg ggcagaatac gattttgaat
     3001 atatccgtcc tattcactgg atcgtggcat tgttagatga cgaagtaatc ccgtttgaga
     3061 tcttggatat caaaacagac agagcttcaa gaggtcatcg tttcttaggc gaagatgtga
     3121 catttgcgca tgcaaaagaa tacgaagaaa aattaaatga acaatttgtt attgcttcac
     3181 cgcaaaaaag aaaagaaatg atcatcacac aagcaaacga attagcagaa aaagatcact
     3241 ggatcattga tctagatgaa gatttactag aagaagtcaa caatctagtc gaatatccaa
     3301 ctgtcttttc tggcgacttc gaagaaaaat atctatctgt tcctgaagaa gtactagtca
     3361 cttctatgaa agaacatcaa cgttatttcg aagtacggaa cgatcaaggt atgttgcttc
     3421 ctcattttat ttctgctcgt aatggcgatc gggtaaaatt ggaaaatgtg gtacgaggca
     3481 atcaaaaagt attgactgct cgtttagaag atgcggaatt cttttataat gaagacaaga
     3541 aagcttcaat cgaccaatac gttgaaaaat taaaaactgt tactttccat gaaaaaatcg
     3601 gtagcatcta tgagaaaatg caacgggtac aagtcatcgc tcgcatcata ggcgaacaag
     3661 taggactgtc tgaggaagaa ttgatggatc tgcaacgcgc tgctgaaatc tataaatttg
     3721 atctagtaac caacatggta ggagaattcc ctgaactaca agggatcatg ggagaaaaat
     3781 atgctctgct taaaggcgag aaaccagccg tagcaacagc gatccgtgaa cattacatgc
     3841 ctatatccag tgaaggagat ttacctgaga ctacagtagg ttctgtttta gcgattgctg
     3901 ataaattaga tagtatcttt tctttctttg cagttgggat gatcccaagt ggctcaaacg
     3961 atccatatgc tttgcgccgt caagcatatg gtgtcgttcg aatcatcgaa tcgaaaaaat
     4021 gggcctttcc tttgtctgtt ctgcaagaaa cgattagtga agttatcagt aaagatacgg
     4081 atcgttttgg cattggcttg tcagcgggac aacaacaggt gatcgacttt atcaaaggcc
     4141 gtctgcgcca attattgaca acgaagaata tccgtcatga tgtcatcgaa gctgttttga
     4201 acgcagaaca aaaagatctg acaaaagtat ttgcagcagc acaattgttc aagcagcatc
     4261 ttgcagatga agacttcaaa ccatcgatgg aagctttgac acgtgtcgtg aacttagcga
     4321 aaaaagcgga gcttgaaaaa caatcagaag tagaccctga actgttcgaa aacgaagcgg
     4381 agaaagaact gcacaaagca gtagaggtaa tgaaaacaag ctttgctgac caaacgatca
     4441 acgaaaatta tgatcagctt gtaagccttc gcccactgat cgaaaattat tttgatcaga
     4501 cgatggtcat ggcagaagaa ccagcagtac gtaagaaccg attaacgcaa ttaagtcaaa
     4561 ttgctgccat ggctttgtca ctagcaagtt tagatcaaat catcacaaaa taatttctaa
     4621 tatgtaaata aaccagagaa caggcacaaa ataggctatt tctctggttt atttgttata
     4681 aatataaata a
//
LOCUS       DACEOE010000173         4669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEOE010000173 DACEOE010000000
VERSION     DACEOE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.178"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            105..1532
                     /locus_tag="IW774_002366"
     CDS             105..1532
                     /locus_tag="IW774_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1154693.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1624..1989)
                     /locus_tag="IW774_002367"
     CDS             complement(1624..1989)
                     /locus_tag="IW774_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1154694.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1986..2351)
                     /locus_tag="IW774_002368"
     CDS             complement(1986..2351)
                     /locus_tag="IW774_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1154695.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2543..2791)
                     /locus_tag="IW774_002369"
     CDS             complement(2543..2791)
                     /locus_tag="IW774_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154696.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2790..2918
                     /locus_tag="IW774_002370"
     CDS             2790..2918
                     /locus_tag="IW774_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1154697.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2975..4669
                     /locus_tag="IW774_002371"
     CDS             2975..4669
                     /locus_tag="IW774_002371"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1154698.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
BASE COUNT         1538 a          890 c          823 g         1418 t
ORIGIN      
        1 cggcatttat atgctttttc agaaattaac tgatgatttc aaaaagaaaa catgtcataa
       61 tatttcccat attgtgcaaa aaaatacaag gcggaggctc tactatgatt gaagaatata
      121 ttgagaaaaa tattttaaga cagctgtttt tatgtgggca attctatgta aataaagaag
      181 tcaatctaga aaagctctcc aatctcctgc atgtttgcaa aaccacttta ctcaatgata
      241 tcaataacat aaaaaaagaa tttgaagaac agatcgctta tacacatcgg gaaaaggacc
      301 gttacacttt atatttttca gaacatatac ctcgatgcaa gatcatgcag caattgtctc
      361 aaaattcgtt gtttttaaaa acttgtctgc tttatttgga agaagatgaa cctgattatc
      421 ttcaattaac tgaatgtgaa ttcatctctg tcagcaaagc ttattcatta aaaaaacaag
      481 tattagctta ttttaatgac tgcgggatcg aaatcgaccg ctactcccct cgattcactg
      541 aaatggaaag acgtctgctg cttcttaatg tgtcgtatcg tttaggcggt tttaattctt
      601 gggagctacc tgaatcgttt tttgaacgtg ccgatcgttt cattgaatcc gtaacggaaa
      661 atagtggcag attttatgat aaagaaaata aagaaatctt atcgattggt tttgctatca
      721 gtttcctacg ccagcaagtg tgtgctgtga caatagattc aaaatttatc gaagaaatca
      781 aaaaacgtcc tatttacaat tatgtcgaat cagcatggga aaacactgat ttccaaacct
      841 attataaaaa agaagaattc gcctttattt tgacactatt caatctttgt aattatggtt
      901 tccattctta tcagttgatt gcggaagact ttcaacagct acaccaagtg tttattgaca
      961 atactcccga aataaaagaa ctagttgcta cctttgaatc tcacttcaat caagaattat
     1021 tcggaaacca accgttcgaa cgtgcgctga ttcatctgat gcgttcggct tgggataact
     1081 atcaattatt catgccagaa aaattttatc ttttaaatga ggaacagaca aatttatata
     1141 aagaagtcca gacaattttt tccagctggt ccagccagtt accttatgat ctacggttga
     1201 atcctaattg catgcgggct tttgtgatcg aattgagcgg aattctccgt ctgacaaaag
     1261 aacatctgac tatctatatc gtcacaaatt cggatgttca ttatctgatt tatcgggaag
     1321 ccttggaagc tgtaactact ttcgattttc aagttgcacc aacaatttat tcaagtatat
     1381 ctgatataaa gaagtatgcg cagcagtcct caaaccgggt attatgtgaa cgtacattat
     1441 atacaccaga tgcggtccaa tatgaaaata ttattcctat ttcgatcaat acgatcgatc
     1501 gagctatcat ttcagctgtg caaaacaaat aaggggctgt gacaaaaatc atgtcacagc
     1561 ctcttatttg ttgaagatgc ccatttctgt aacaatctct tgatacacgt caggctcaca
     1621 acctcactta ttttggacat ctacgggtcc gatctcttta tttaaaaaat aagaaatgat
     1681 ggttcggata actaccaacg ccgctagttg gaaaatatcc tgcaaggtag gtttgataat
     1741 cgattcgatg atcccagctg aaacaagaat ttccaaactt agaagtatat agcctcccaa
     1801 catcttttta atagcattgt tctcgctgac tcggtcgctt tgcacttttg tatggacaaa
     1861 taaatatttc aaaaaatcct tgattgatag acaaacaccc caaaccaata aacagatagc
     1921 aaagatatca agtcccagta caagtttttc gaaatatgga agaagaaact gcatcaactc
     1981 tgtcgtcatc ctacctctcc tcttctttgt ttttctttgt cttctatttc ctgattcaag
     2041 aaataagaaa tgactgttcg aatgataata ataagtgcaa gcttcaaaat atctgtccaa
     2101 gtcggcttga taactgactc gatgatatca gcaacgacta gtacttccaa gcttaacaaa
     2161 atataacttg ctagcagttt tcttgtatct gcattacgag tattacgttc tgcatagttt
     2221 ttcttgacat gagaccaatc aaaaagtttg aatagaacaa gaacgatccc gacaattaag
     2281 ataccgatag aacaaatatt taaaatagca atgatccatt ctagaaattc aattaaattt
     2341 gagcgaaaca tatacgctac ctactttctt ttaatttgtt cctattcttt tattgtaaaa
     2401 gaaagttacc ttgttcgtct atttaaaagt tgttctaatg ataacggttc gcttaaattt
     2461 ttctttttca ctctcccatt tttctccctc catccataaa tcgtggaagt atccaaaaca
     2521 acgtagcaag ttaaaataag gcttagtccg tcatcagctt ttcaaagaag cactgtctcg
     2581 gtgctttttt tgtacccaaa tttctaaaaa tagaaccata cacctcctca aatcttttct
     2641 tcttacctac cctattttta tttacaggaa aatcagacac ttcttctttt ttgtcaaaat
     2701 ttcctttttc ttctcgtcct atcatttggc attctaataa aaacgaacta tccttggata
     2761 tagaaagaga aaacaaggag gcgtttttca tgagtaaaaa attaatcaaa gtcggcatcg
     2821 gtttaggttt gcttgccctt ggcgctgcat atctaggaaa aaagacagga ctattcgaag
     2881 atgacagtca tttatatgat gaattcgaat caatctagca tcctatttag ttgaatcact
     2941 acggacatac ttggaagaca ttggaggaat cacaatgaga aaaagtaaag caataatgat
     3001 tggtgccggt attgccaata tggcagcggc tgtttatttg attcaagaag gcaaatggaa
     3061 aggggatcag atcacctttt attcattgga tgatcacggt tcaaatgatg gtgcaccgac
     3121 cgacactgta gtggatgaat attggaataa aaaccatcca ttagaaaacc aaaaaggcta
     3181 tattgctcga ggcggtcgca tgcttaatta ccgtacctat gtagatttga tggatctgtt
     3241 aagtcgaata ccttctgtta ctgagccagg gatgactgct gaagaagata cgagagattt
     3301 tgatgcaaaa caccgaacct ttgacaaagc acgtttgatg gaaggtggca aaggaatcat
     3361 tgatggcgga aaactgggat tcaataataa agaccgtatt cttctgacta aactgattgc
     3421 tatgcctgat tcagaagaag aaaaactaga caatatcact atcgaagaat atttcaaaga
     3481 tgatccacac ttttttgaaa caaacttctg gtttatgtgg gaaacaacat ttgctttcag
     3541 aacacgtagt tctgctcaag aattgcgtcg ctatatgcat caaatgattt atgaattcac
     3601 tcaaatcgaa catctagtgg gcgtcaatcg aacacgttac aatcaatacg aaagtattat
     3661 gcttcctttg atcgagtact tgaaagaaca aggatgcaaa atcattttga accgccgtgt
     3721 cactgactgg gaattcaaag aaacaccgat gcaagatgaa atcactgtga ctggtttgaa
     3781 aattaccaat acactgactc aagaagaaga atacgttgtg gtcgatgaag atacagcagt
     3841 tattttcact aatgggtcca ttacagattc agctacttta ggagattacg aaacaccagc
     3901 tgtagaaaat atggattatg gtgcagcttc tagtctctgg aaaaaagcca gtgaacgttt
     3961 ctacaacttg ggaaatcctg acaaattctt tgctgatcga gatgcaagcg aatgggtcag
     4021 ctttaccctg acgacgaaaa accatttgtt gttgaatgaa atcacccgta tcacgactca
     4081 agaaccagga aatgccttga attcatttat ttcaaccaca ccgatcacgc cgttaggcca
     4141 aaaagatgtc aatatgtcta tcgtcgtgca tcatcaaccg cattttacgt ctcagaaacc
     4201 aaatgaaaca gttatttggg gttatttcct ttatccaaga cgacgtggcg aatttgtgga
     4261 taaacagtat attaaaatga atggaaaaga aatgctagaa gaactgattg gacaactttc
     4321 aaaagtagat cctggaccag tgaatattcg tgaaaaagaa acagaaattt tcgacagcgt
     4381 aataaataat atccctgtct atatgcctta tgcttccgcg ttgttcaata atcgtgccaa
     4441 aagcgatcga ccaaaagtca ttcctaaaca ttcgaccaat ctggccttta caggagaatt
     4501 tgtcgagcag ccttatcaga tgatctttac tgaacaaagt gctgtacgtt ctggagaaat
     4561 cgctgctttc cattttgcgg gtattcctat gtcccgtctt gtcaaaacac cgcgttacga
     4621 caaagatatt cctactctag ctcgtgccgc aaaaaaaatg tttgaataa
//
LOCUS       DACEOE010000174         4641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEOE010000174 DACEOE010000000
VERSION     DACEOE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.179"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..498
                     /locus_tag="IW774_002372"
     CDS             <1..498
                     /locus_tag="IW774_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154699.1"
                     /translation="SIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGK
                     KETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFE
                     KTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLV
                     EYGII"
     gene            485..1906
                     /locus_tag="IW774_002373"
     CDS             485..1906
                     /locus_tag="IW774_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1154700.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            1919..2926
                     /locus_tag="IW774_002374"
     CDS             1919..2926
                     /locus_tag="IW774_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1154701.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            2938..3942
                     /gene="neuB"
                     /locus_tag="IW774_002375"
     CDS             2938..3942
                     /gene="neuB"
                     /locus_tag="IW774_002375"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ1154702.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            3939..>4641
                     /gene="neuC"
                     /locus_tag="IW774_002376"
     CDS             3939..>4641
                     /gene="neuC"
                     /locus_tag="IW774_002376"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ1154703.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQ"
BASE COUNT         1639 a          594 c          827 g         1581 t
ORIGIN      
        1 tcaatctttg ggatagaaga agtttttgtc ggagataaat gtatcttttt tgatcaacct
       61 ttattatctg atggttatgg catagatgaa acagatattt tcgctaaagt aaatagtata
      121 ttttctggaa aaaaagaaac tttaattaag cttcatccta gagcaaggac aaatcgttat
      181 ggtgaaaaaa acatattgaa ttccagttta ccgtttgaag tcttgctact tgaaaatgac
      241 ttctcttcaa cacttttgat tagtcctata tctacaattt ctttttcacc atctatgatg
      301 tttgaaaaga cgatcaactc tattatgttg gctaaattaa ttttaaatga atatacagca
      361 ttatctgaag ataaaaaaga aacattgaca aatattgttc ttttctgtga acattataac
      421 cgtctatcag aagaaaaaat tttactccca gaaaactggg cagaattaag aaagttggta
      481 gagtatggga ttatttaaca cactttccaa gtcaaaatta gttaagtcca gtttctggta
      541 tactataggg aattttttta ttaagggaat ttcattcata actattccta tttttgtaaa
      601 tattactact caagcagaat atggtttgat taataatttc acttcatttg tatcaatttt
      661 ttcgattctt gttgggctaa gcctaaattc ggcaattaat aatgcaagtt ttgactataa
      721 agatgacata aatgaattta tgtcatcggt actttctcta gctacacttt cgttaattgt
      781 gttcttatta atagggaaca tatatttttt gttttcaaat gctttttttg atatgaacca
      841 acttgtattt aatttgatgt tattacaaag ttttgctagt tttgtaatta gttatatcca
      901 gtcttatttt acgattaact ttaaccattt taaatatttg attttatctt ttatatctac
      961 ttttttgaat attgcaatct ctgtagcttt gatgcagaca ttatattttg atgataaata
     1021 tgttggcaga gtagtcggcg gaacactgtc atttgtgttg ataggtttag taatttattt
     1081 aagaataatg ataaagggtg ctcatttttt tgataaaaaa cagtggagat acgctttaaa
     1141 aatctctttg ccgctaatcc cacattcctt agcaaatata gttttagcac aattcgatag
     1201 aattatgatt aattcttatt taggtcctgc tgcgggggga atatattcct acattagtaa
     1261 cataggtatt attctaagcg taatatggat gtctactaat aacgcctggg ttccatggtt
     1321 ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag aaagttagta actattattt
     1381 agtagtcttt actttgatta cgatgattgt aatggtagtt tcgattgatt tagcaaaatt
     1441 tatggctccg aaagactatt attctgggtt atctttagtg gttccgataa ccttaggcta
     1501 tttctttcag tttttatata gtttaccagt taatgctgaa ttttatgaga aaaaaacatc
     1561 gtttattgcg attggtacag tagcaagtgc tatagttaat atactattaa acatgatttt
     1621 catacctagg ttaggaataa ttgctgctgg atacacaacc gtatttacgt attttttact
     1681 ttttattttt cattataata tcgctaaaaa aattacgaat aaacaattat ttgacacaaa
     1741 aaaaatatgt atcataataa tttttgtatc aattttttct ttgatacttc atattttaat
     1801 caatcaaatg ttgattcgtt acgttttgac atcattattg ataattagtt tagttatata
     1861 ttttttaaag attaagaatc gaattataga tgactataat tcataggagg agatataagt
     1921 gtttaaagga aaaacaatat tagtaactgg cggaactggt tccattggaa gtgaaattgt
     1981 tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt tatagtagag gtgaagaaaa
     2041 acaattttat atgcaacaag agctctcaaa tcacactaat ttgagatttt taattggtga
     2101 tatcagagac tctcaaagga ttgattatgc atgtcgtgat gtggatatta tttttcatgc
     2161 tgccgcaatg aaacacgttc cagcaagtga atataatcct atggaagctg taaaaacaaa
     2221 tgtgatggga actcaaaatg taatagatgc agcgatcaaa aataatgtaa aacattttgt
     2281 aggtatcagt acagataaag tagtgagtcc tactaacacc atgggtgcga caaagttatt
     2341 gagtgaaaaa ttaataatag ctgccaatca gtataaagga aaaacagaaa caatattttc
     2401 ttgtgtaagg tttggaaatg tcatggggtc aagtggatct gttattcctc tttttagtaa
     2461 acaaataaaa aacaataaag atattacttt gacagatgtt gatatgacac gatttatgat
     2521 gtcaataccg gatgctgcaa gattagttct gtctgctgct atgaattcaa aatttggaga
     2581 aacatatgtc ttaaaaatgc cggcaatcaa tattttaact ttagctcaat cattaataaa
     2641 aatatataat gatacacatg atgaaaaata tagaggtgaa ataaaagttg taggtatcag
     2701 gccaggagaa aaattatatg aagagctaat gactacagac gaaattgaac gctcatatga
     2761 aaatgataaa atgtatatca ttccaccaca ctatcatgct actgacgaac tgtacaaaga
     2821 ttatgaaaaa attaaaatca ctgcatactc ttcagatgca acagaattaa tgagtggaca
     2881 ggaatttatt agaatcgcaa aagagaataa tgtcgtgact tggtaaggag ttttatgatg
     2941 aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag tctagattta
     3001 gctttagaaa gtattgataa agctaaagaa tgtggagcta acgcggttaa atttcaaaca
     3061 tttaaaacga ataagctagt ttccaaaaag gctccaatgg caaaatatca gaaggataat
     3121 cttaaagagg acagttctca atttgaaatg ctaaaaaaat tagaattatc tcaatctgat
     3181 tttattagaa taaaagatta ttgtatacaa aataaaattg attttttgtc tactcctttt
     3241 gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa aattggctca
     3301 ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt gccagtttta
     3361 ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg tttcatagat
     3421 agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga agatattaat
     3481 ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta ctccgatcat
     3541 tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa
     3601 cattttacct tggataagga actacctggt ccagatcata aagcttcttt agatcctcag
     3661 gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg tgatggtgtt
     3721 aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag cctagtggtc
     3781 actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat aaaacgtcct
     3841 ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt taaaagacca
     3901 ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata cttatagtaa
     3961 gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta ctacaagata
     4021 attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat ggatttacta
     4081 aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt ttgtttttag
     4141 agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt ttcacaaata
     4201 tatttgaaca aactagccct gattttgtat tattactagg ggatagagga gaaatgttgg
     4261 cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac ggaggcgaag
     4321 tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca tctgttcatt
     4381 ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat tcttggagag
     4441 tttttcccgt cggagctcct agaatagata ctattttaaa taatagatta cctagtttta
     4501 gagatataaa agaaaaatat catctaaaat ttgaaaagaa aaaatacgtt ttgcaagtat
     4561 ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca aattttagct ttgatcaatt
     4621 caatggaaca acaatctcaa c
//
LOCUS       DACEOE010000175         4606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEOE010000175 DACEOE010000000
VERSION     DACEOE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.180"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            315..1448
                     /locus_tag="IW774_002377"
     CDS             315..1448
                     /locus_tag="IW774_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1154704.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1479..1799
                     /locus_tag="IW774_002378"
     CDS             1479..1799
                     /locus_tag="IW774_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154705.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(1828..2292)
                     /locus_tag="IW774_002379"
     CDS             complement(1828..2292)
                     /locus_tag="IW774_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1154706.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2424..3678)
                     /locus_tag="IW774_002380"
                     /pseudo
     CDS             complement(2424..3678)
                     /locus_tag="IW774_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(3707..4024)
                     /locus_tag="IW774_002381"
     CDS             complement(3707..4024)
                     /locus_tag="IW774_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1154707.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(4050..4370)
                     /locus_tag="IW774_002382"
     CDS             complement(4050..4370)
                     /locus_tag="IW774_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1154708.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         1463 a          936 c          825 g         1382 t
ORIGIN      
        1 ataccgagaa actgatccct tctgtcataa cctcttttta tttatgtctg tttctttttg
       61 aaaacaacta ttttttcaac gtttactctc ttgccaatcc aacatcatct aacggataca
      121 actatttcaa tgattaccat tacactaaaa aatcggaaac acacctctcc gaacacaaat
      181 acataagact atagaatgtt tcatttaaga aaaaaagcgt ttcattatca aaaaggtaag
      241 atctccttta aaatgaaatt atctgtagta ctaataatgc agacaatttt atgaatctga
      301 aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg gaaatcagaa
      361 acgtagatga tccaaaaata ttagaaccaa cagatgcaat catccgtatc acagcaacag
      421 ccatttgcgg ctccgatctg catctttacc atcatggtga agcagttttg gaaaaagggt
      481 atgttgtagg acacgaacca atgggaatcg ttgaagaagt gggatctcaa gttcaaacat
      541 tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag ttgtggtcat tgtcatttct
      601 gcctgaatca catggaaagc caatgtgatg aatcaaatcc agatcagtta tatggcgggc
      661 ttttcggatt tggcaagttg aacggtaatc attggggtgg tcaagcggaa tatctaagag
      721 ttccttatgc agactctact tcatttgttg taccagataa tgatcttcct gatgaaaaag
      781 ttttatttct atcagatatt ttgcccacag cttattggag cgttgaaaac gcaggagtaa
      841 aaaaaggcga tacagttgtg attctaggtt ctggtccagt cggtctattc gctcaaaaat
      901 tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga tccagtacag caccgtctag
      961 ataaagcggc taattacaat ggggttgaaa cttatttctt agaagacacg gcacaagctg
     1021 gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt cattatcgat tgtgtaggaa
     1081 tggacggatt agagcctgtc aaagaaaaag cgaaaaattt agtaagctta caaagcggca
     1141 caatctctcc attacagatg gcatcgcaag ctgtcaaaaa atttggaaca gtccaagtaa
     1201 ctggtgttta tatgactcca gcttcttctt acccgctgca agaatttttt atgcgggata
     1261 tcgctgtcaa gcatggacag gcaccaatta tccatctaat gccaaaaatt tacgcaatga
     1321 ttgcagaagg actattcgat cctacacaaa tcattactca ttcgctgcca ctatcagaag
     1381 cagcacaagc atatgagatc tttgacaaaa aagaagataa aaatataaaa gttgttttga
     1441 aaccttgaat tcagtaaaaa atacaggtga ttttctttat gcccaaaatc aaacgaaata
     1501 cccatgagat cgaaatagga aaagaagaag atatcgaaat acgaggacgt cgcttccgct
     1561 tctatttcca aaatcgttac acattgatct cactggctgt cgatttatta accgggattt
     1621 tctatatttt tggaagcctt gcttccttaa ctaaaatccc cgatcgatat ggcacctatt
     1681 tttatttggc tggtgctatt ttcttgacga ttcgtcctat tctcagaatc gtaaggaaca
     1741 tttttattta cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa gaggcatgag
     1801 aatttttcaa atctcatgcc tcttttttca ccctatcgta tcactttgat acagacgagc
     1861 ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc cactcgtatc gttcataaaa
     1921 cgtggtatga tctgtaatta agtaaagttt ttcgtaccca agttgatgca tttcttttct
     1981 gatttccgct aatatccgct tggctatccc ctgcttacgc ttttccggtt caacaaacag
     2041 ggcacaaaga tttggtgtca gatcagggcg gttatggaag tcgttttcaa tgacgcccgc
     2101 tcctccgacg atggtttctt tttcatctaa gaccacatac cattgaggaa tactttcctt
     2161 attagcgatt ccagtacgaa tactttcctc atacacaaca gcaggaactc cccattttga
     2221 tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt tgatccagtt
     2281 atatgtaacc aatgtttaaa cctccgacta aaataaaacg tttacaaaaa ttatacaagt
     2341 ctttatgaaa attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg aaaacaatca
     2401 cttgttctcc gttttttgat tgatcaagca tctaaagctt cttttcttct ttcttggatc
     2461 gtagctacct tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt gacagcggct
     2521 agtacaccac ctgcaatact ttttgttgcc aatacaccgc caataattgg tggagttacc
     2581 catggaggca taacggtagc tgctggcact aaacctgtag aagtcgcaaa gtaagcaaca
     2641 ctaactaaga ccattggtgt caaaataaat ggaatgaaca tgatcgggtt caacacgata
     2701 ggtaacccaa acatcgttgg ttcattaata ttgaaggaca cctggcgcaa tcgacaagtt
     2761 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
     2821 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
     2881 aaacggtgcg ctatgcccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
     2941 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
     3001 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
     3061 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
     3121 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
     3181 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
     3241 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
     3301 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
     3361 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
     3421 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
     3481 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
     3541 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
     3601 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
     3661 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
     3721 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
     3781 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
     3841 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
     3901 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
     3961 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
     4021 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
     4081 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
     4141 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
     4201 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
     4261 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
     4321 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
     4381 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
     4441 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
     4501 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
     4561 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggt
//
LOCUS       DACEOE010000176         4599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEOE010000176 DACEOE010000000
VERSION     DACEOE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.181"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(61..2727)
                     /locus_tag="IW774_002383"
     CDS             complement(61..2727)
                     /locus_tag="IW774_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1154709.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            3068..4519
                     /gene="cls"
                     /locus_tag="IW774_002384"
     CDS             3068..4519
                     /gene="cls"
                     /locus_tag="IW774_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1154710.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1344 a          978 c          833 g         1444 t
ORIGIN      
        1 ttgaaattgc accttgtgac tcagaattaa acggcaggct cacaacctcg ttgattcgtt
       61 ttatttattt ccttgtactc gtacaatgat caatttagtg atttccatca aaacaattgt
      121 cacaagtgcg attcccatac atgtaaagac ttctgtcaat ccaaatgctg atgggataga
      181 gaagacttct ttcagtcctg ggatcattgt taagcagtaa agcgcgctac atacaactac
      241 agctagtaag acacttctgt tagagaagaa ccctgcttgg atagctgttt gtgtgtttga
      301 acgtgctggg aatgtttgca atgtccgaca ccaaatcaat gttgaaaatg acatggctac
      361 accgaattca ggtgaatgct gcatgccaag ccattgagca aagataacag caatcccaat
      421 cagcacacca cgatacgtta cagaaatcaa tgttttgtcc gtaaagattc ctgaatttgg
      481 atctcttggc gggcggttca tcgtagcagg ttcagctttt tctgtaccta atgcaattgc
      541 tggcagtgaa tcattgacta aattgatgaa caacagttgt aatgctgtaa atggattgga
      601 ccagtcagca actaaggcaa agatgatcgc aatgatcgca cctaaatttc ctgcaaatag
      661 ataagaaata gctttcttga tattatcaaa cacattacgt cctacttcta ctgcgctgac
      721 aatagaagca aagttatcat ccgtcaatac catcgctgct gcatcttttg ccacatctgt
      781 acccgttccc atagcaattc cgatgtctgc ttgtttcaat gctggtgcgt catttacacc
      841 atcacctgtc atagctgaga tatgaccttt attttgccaa gcacgaacga tacgaatttt
      901 attctcaggt gaaacacgtg cataaacagt gactttttct aaaacttcgt ttagttgatc
      961 ttctgtcaat tcatctagtt ccgtcccggt caaagccagg tttccttctt caaaaatccc
     1021 gatttgtttt gcgatagcaa cagctgttgt tttgtggtca ccagtaatca tgattgtttt
     1081 gattcctgca cttcttgctt ctgttaccgc ctgagtcact tcagcacgtg gaggatcgat
     1141 catagcaagc aaaccaacaa ggatcaaatc gtcttcatct tcaaatgata aatcaaaatt
     1201 atcaagtgga cggtaagcaa aagctagtac acgtaatgcg cgtgttgaaa aagcttcatt
     1261 ttgctcttgg aattttttca tcaattcatc cgtcatcgga acgacttcac catttagcaa
     1321 tactttttta ctgcgttgga agacgatatc tggtccgcct ttaacaaaca agtaattttt
     1381 gttatcaatt gtgtgtccag tagacattaa ttttcgatct gagtcaaaag gaagttctgc
     1441 ttgtcttggg tagttgtttc ggatttcctg ataaggctgg tttaattttt cactaaagtc
     1501 gataaacgct acttccgtag ggtcgcccaa tttagtaccg tcttcactga tcgatgcatc
     1561 attcgctaaa acactgattt cgatcaagcg acgttcgtca gctgtccatt ttgaagggtc
     1621 atttgtgaaa tccccagtat cgccattacc taaaaagtag tcaacaactg tcattttgtt
     1681 ttgtgtcaat gtaccagtct tatctgtaca gatgatactt gtcgaaccaa gtgtttcaac
     1741 tgctggaagc ttccgaataa tggcatgacg ttttgccatt ttattcgttc ccaaagataa
     1801 cacgattgtt acgatagact gtaatgcttc tggaatagca gctacggcta cggctacggc
     1861 aaacatcact gcgctgacga tatctgcagt catatctcct gtaccttctc caagataaat
     1921 acgaaacagt tggataccta aaatcaataa agacaatgcc aaaatagcca aacttagttt
     1981 tttactgaat ttatccagac ctcgttgtaa cggagtgact tgctcagttg ttgattccaa
     2041 aaggccagca acttgtccga tctccgtatt attcccagtt gaagtcacta agaatttacc
     2101 gcgtccgtaa gttacgatcg ttccactgaa taccatattt gtacgatctc cgattggtac
     2161 cgtacttgtg atatccgtta cttctttttc tgcagaagtc gactctcctg tcagcatacc
     2221 ttcatctact tttaatgatc ccgcatctag taaacgtcca tcagcaggta cataatcgcc
     2281 tgcgtctaac aacacgatat ctcctaccac aagttcgttt gccggaatcg ttttttcctt
     2341 accatcacga atcactcgag catcgggcgc tgaaaggttt ttcaacgctt ctaaagaacc
     2401 ttctgctttt tttgtttgga caacactgat tatagaattc aacatcaata ctgcaaaaat
     2461 aactaagaat tcgatgtagt cacccatgac catctgaatc agtgcgacaa tcagcaatac
     2521 gataaccatt gcatctttga aagtttctaa gaacaatttc caagttggct tcttcggctt
     2581 ttcttttagc tggttcaagc catctctttc taatttttcc ttcgcttgcg tggtgctcaa
     2641 tccatcggtt gagctctgcg tttgctgcat caacgagtcg atcgtttctt tatagaccgc
     2701 ttgttgtgct tgttccttgt tttccaaatt tcatccccca ttttttgatc ttggccctag
     2761 tcctgtctga aaaaaaagag acctaagaca aatacgcatt tgtcttaggt ctcaccattt
     2821 catacaatac cagaaaacca tttggttaac ttactgttga tattatcgca acaaacgttg
     2881 ccggttactc ccctaaggag atattcaact gtactaatat cctaccttat taacctacaa
     2941 aaagcaacta ctttagcccg atttacctac attttcatga aaggagtgaa aagtatattc
     3001 accttggtag gcttctttct tctatcatgc tatactaatg ttattgtatg taaggaggaa
     3061 catttttgtg gtatctagta ttataaccgc cctttatcta ttaaatgcac ttattgcttt
     3121 ggtggctatt ttgatcaaac cccgagatgt agcagccatt tgggcatggc tcttagtatt
     3181 gtttgccctt cctggggtgg gctttgtatt atatttattt ttcggacgtg gattaacgga
     3241 taagaaaaaa ttctatctcc gacaaagtga cttgaaagaa ttagaaaact ttcagtcttt
     3301 tagaggagat accattgaac attacgatcc tgacatgggc gataaagaca aacagcaatt
     3361 tgttgacttc ttctcttcat taaatcgtat gccgctgaca agaatgaatt ctgtcactct
     3421 tctcacagac ggacaagaga aattggattc actgcttcaa gatctaaaaa aagccaaaca
     3481 ttcgatccat atcgaatatt acgcatttgt gacagataat atcggccagc aagtcttaca
     3541 tgttttagaa gaaaaagccg cagaaggcgt ggaagttcga atattatatg atgcatttgg
     3601 ctctcatggc acaaaagcaa aagatttcaa tcgtctaatc aaaaatggtg gacatgtcca
     3661 tacatttgtt acctcacaaa gggcattact tcgtttccga ttgaattacc atgatcaccg
     3721 aaaaatcgtt gtgatcgatg gaaagattag ttataccggt ggtttcaata ttgccaatca
     3781 atatgtaaat acaacaaaaa aattcggcta ttggcgcgat acgcatatac ggattttcgg
     3841 tgccgcttct tctttgctcc agcttcgctt cttaacagac tggaacgtct cggtacctga
     3901 agaaaaaaag gtcggctatc atttgaatta tttctttaaa aaagcagatc gagatgaatc
     3961 taagcttgct gatacatcca tccagcttgt ttcaagcgga ccgaataacg aaagggaaca
     4021 aatcaagctt tcatttatca aattgattac ttctgctaaa aaacgtgttt ggatacagac
     4081 accttacctt gttcctgatg aaagtgtcat tgctgcttta aaaatcgcaa ctgcctctgg
     4141 tgtagatgtg aaaatcatga ttcccaacaa accggatcat ccttttattt atcgagcaac
     4201 acaatattat gctcggcagc tgatcaagga aaatgtacaa atccttgtct atgagaacgg
     4261 cttcctccat gcaaaaacat tgataatgga tgatgaaatc tgcatggtag gttcagcaaa
     4321 tcaagatatt cgtagctacc gattgaattt tgaaacaagt gctgtcattt acgatcctga
     4381 gtttttagaa gaacttgcta ctcagttcaa agaagatgag acacattgtt catccatgac
     4441 aactgaaaca gtcaaggaaa tgtctaactg gctattattc aagcaacaaa tttctcgatt
     4501 attttctcca atcctataat ttttttattc aagtagagaa aaatatacta agattaaagg
     4561 gactgtgaca aaaatcttgt cacagtccct tcttccgaa
//
LOCUS       DACEOE010000177         4519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEOE010000177 DACEOE010000000
VERSION     DACEOE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.183"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(92..1141)
                     /gene="recA"
                     /locus_tag="IW774_002385"
     CDS             complement(92..1141)
                     /gene="recA"
                     /locus_tag="IW774_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1154711.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1250..2491)
                     /locus_tag="IW774_002386"
     CDS             complement(1250..2491)
                     /locus_tag="IW774_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1154712.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2731..3309)
                     /gene="pgsA"
                     /locus_tag="IW774_002387"
     CDS             complement(2731..3309)
                     /gene="pgsA"
                     /locus_tag="IW774_002387"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1154713.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3518..4402)
                     /locus_tag="IW774_002388"
     CDS             complement(3518..4402)
                     /locus_tag="IW774_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1154714.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         1212 a         1029 c          818 g         1460 t
ORIGIN      
        1 aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag tacctactct
       61 tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc aattcttctt
      121 gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct gctctaacta
      181 gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta gcgttttccc
      241 gtccttgacc aatccgatct tctttataag aataccaagc accgctttta tcaacaatgt
      301 ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa ccatacatga
      361 catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact ttgatctttg
      421 tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt acttccaaac
      481 ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca
      541 tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt gttttgttga
      601 tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg
      661 agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact gaatcgacta
      721 ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga ccttgttctc
      781 ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt tgtgcatatt
      841 gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt ttttgtactt
      901 ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca tatacttcga
      961 tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca agagagccac
     1021 ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata gagcctttac
     1081 catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga tcatctgcca
     1141 atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc ttatcatatc
     1201 ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat cattttctta
     1261 aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg atagcagcac
     1321 gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc
     1381 atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg gtgaaagcta
     1441 cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc acacattctt
     1501 tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa ccagcttttg
     1561 tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca gcaatgtcgc
     1621 ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca gtctgcttgt
     1681 tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg tatccataaa
     1741 aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct tgattccctg
     1801 cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt ttggcatatg
     1861 gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg
     1921 attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca gaaggaaaac
     1981 gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca ttaggcggac
     2041 cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa gcaagccctg
     2101 tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga ttgtttttgg
     2161 tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct tccgtatttt
     2221 gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg
     2281 gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga gcaatcaact
     2341 cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg atacctagat
     2401 cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct agtaaaagtt
     2461 ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata tagtatacgc
     2521 tgtattttta tattttttta tttaaaaaga gggcaaggag ctgtgacaaa aatcttgtca
     2581 cagctccttg ttccaaataa acagtgttct cctgccaaaa cctcggtcat aagtcacaat
     2641 atacataaaa catgaaaagc tgttttttga aattatccct tgtgactcag agttagacgg
     2701 caggctcaca acctctccac taaacgatac ttacattgat cctttgaaca caccgctgtt
     2761 tttgatgaag taatccacac ctgaatagat agtaaagatc agacaagcat aaagcatgat
     2821 ttgatctact ggaataccga tcaggctgaa tgggatatta ttgataaaca gcaagataat
     2881 agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gctgcagcca tgacttctcc
     2941 atgttcgaca agtagtaaac gcaagcctgt gacggctaat tcacgacaga caatgatcgc
     3001 tactacccaa ccaggggcct ttccttggtc aaccaacatg ataaaagcag tcataactag
     3061 cattttatct gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc ctcgcgcacg
     3121 ggcaattttt ccatctaacc agtctgtgat actagctaaa gcaaaaataa ctgcacctac
     3181 tagctgtgtg acagccagtg tggtgtctcc tacgttaaat tgtccccaat ctaaaggtaa
     3241 acctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta atttatttgg
     3301 taaattcaca ctatcgactc ctttcttccc tattctacgt gactatcagc gaagtttcca
     3361 ccgatattat ttacttttcc aaatgaattt attcaaagga gtatttttat tgaaattgat
     3421 ctttttgaat taacgaagaa ggaccgcgac acagatttgt cacgatcctt cttttttata
     3481 cctacaagta aacagatgcg taaactaatt tatacgatca ttctgcgtat tgaagagtaa
     3541 ggttcacatt ttttggactc tgattgtttt cccctggatt aactggtacg gattctccat
     3601 tcgctttgat cgttgcattg cttgctgcac caatcgtgat ggtcgcattt gttgctgttt
     3661 ccggtaacac ggtactctgt gtttcatttg ctgccagtgt cccttgataa acataggcat
     3721 tgtctactag cacacctaac catacacgat tgacagcagt aaattcaagg gttacaggct
     3781 tggtagcgtc tgtgatattt actgcgatgg cactgccaga atcttctcct gtagctattg
     3841 ccattttctt ttcttcactt gattttgttg ttgattctac tgtagaagac tctttggtct
     3901 gcgtgctttg ctctgaggaa gaggactcct cgactgatcc ttctacttta agcgaagaag
     3961 ctgtgtctcc gattattgga tcagcttgac gatcttgcca agtcatatag cccacgacaa
     4021 cgacaatcgc caaagcaacc aatcctaata aaatgacagg aagacttgtc caaaagcgac
     4081 ttggatgcga ttcttcctga tgcattgctt ttctagaagt acttactgtt tcaggctgcg
     4141 gacgttcagg gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg actaaatggt
     4201 ctccatcttc tcctacagct tcagcgtatt gccgaataaa tgcccgcacg taaaatttac
     4261 caggcaattg gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt tgaatttttg
     4321 taatctgctg taattcatct aaagatattt ttttattcag gcgtgcttgc cgtaattttt
     4381 ctccgatggt ttcaaaagcc actcgttcat cttctccagt ctgtttgatt ctccgaagca
     4441 tctgctgttg caacgaaaga cgttatgttt gggtgctgtg acaaaagtca tgtcacagct
     4501 ccttttttcc gaataaaca
//
LOCUS       DACEOE010000178         4507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEOE010000178 DACEOE010000000
VERSION     DACEOE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.184"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..353)
                     /locus_tag="IW774_002389"
     CDS             complement(<1..353)
                     /locus_tag="IW774_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1154715.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKSNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEP"
     gene            503..1453
                     /locus_tag="IW774_002390"
     CDS             503..1453
                     /locus_tag="IW774_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1154716.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            1446..2405
                     /locus_tag="IW774_002391"
     CDS             1446..2405
                     /locus_tag="IW774_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1154717.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            2402..3157
                     /locus_tag="IW774_002392"
     CDS             2402..3157
                     /locus_tag="IW774_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1154718.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            3180..4133
                     /locus_tag="IW774_002393"
     CDS             3180..4133
                     /locus_tag="IW774_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1154719.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT         1342 a          765 c          943 g         1457 t
ORIGIN      
        1 ggttcattta ctgcttcttt tgctgttaag agcaatccga attcctcagg atttgcagtg
       61 acgtaatcgt tgactacagt gaaacggcta ttgcttttcg ttgctagctg gatatattgg
      121 ttttggacag aaaggggtag agccccattc aagagtatgg aagcatctgg atgttttttg
      181 agttctttta aaaagttttc tttgtttttt ggttggatca tttcagcaac agtcatacta
      241 agatagcagc gttccctgaa tgtccctaaa tatttatgct gttcttcagg gttcactaaa
      301 ggtgtaccat aaagtccctt atccaaatgg ctttttacat catcttgtgc cataaaaaat
      361 accctccttc ttctctttgt ctttcttacg attatagcat attcccaata atacctaaaa
      421 catatgaaaa agcggttgct tttgttcaac gattcactta aaataagaat gaaaatcatt
      481 ctcaattaga aaggtggatt ggatgcgcta cggacgacta gtagcattat tagttatttt
      541 aagtagtatt tcattgttca tcggagtatc gaatgtacaa ttgtcagaac tgctccagtt
      601 agatgggcaa agcttacaaa tcttgctagt gactcgtttg ccacgaacga tttgtttgat
      661 ccttgtggga gcgaccagta gtatttgtgg gttgatcatg caacatttga cccaaaataa
      721 atttgtttca ccaacgactg cgggcacgat ggatagtgcg cgtttaggta ttttagtggc
      781 tatgattttc ctacctggtg catccttgat tgtccgatcc ttgacagcat tttgctttgc
      841 ttttgcagga acattgcttt ttttatccct tacgcgattg ttcctccaga aaaatcaatt
      901 gatgcttccg ttgattggag tcatgttagg gaatatcatt ggttcagttg ctactttttt
      961 tgcttatcaa ttccagttgg tacaaaatat gacttcatgg cttcaaggga attttgcgac
     1021 tgtgatacga ggaaattatg agttattgta tgctgttgtt ccattgttgc tcttggtttg
     1081 gctatttgct taccgattta cgatcattgg tctgggcgaa tcgtttgcag taaatctggg
     1141 aatagattac cagaagatgc agtttttagg cattggctta gtatcattgg ctagtgcagt
     1201 catgttgatc atggtaggaa gcattccttt tatcggagtg atcattccga atcttgtctc
     1261 tagaatatat ggagatcaag tgcacaaaac aattggcgta actgctttag caggtagttt
     1321 gttcttagta ttttgcgatc ttgtcgctcg cgtgttgatt tttccttatg aaataccagt
     1381 cagtgtgatc gttggaatca tcggtggggc tatcttcctt tatcttctga taaggggacg
     1441 acgatatgct taaaaaaaag ccatctattt ttattggtat gcttgttctt gttttatttg
     1501 ttacaggaat gtacttgacg atccatacat atggaaactg gcattttgca tgtgcattga
     1561 ggagtaagaa aatttttgca tttatccttg tctcgctagc tgtcagcagt gccacaatca
     1621 gctttcagac aattacgcat aaccagtttt tgactccttc agtattggga cttgagtcgt
     1681 tatatgtcct tgtccaaacg gtccttttct tctttatcgg aggagtgacg atgcttcagc
     1741 agacaggtac agtttttttc ttattgaacg ttgggctgat ggtcgggctg agtctattgt
     1801 tatttttctt tcttttgaaa aaaatgagag cgaatctttt tttattactg atgtcaggga
     1861 tgattttagg tacgcttttt ggtagtgtca gtaccttttt acaagtcatc atggatccga
     1921 atgaatatga tttgcttcaa ggcagattat ttgcaagctt tggcaatatc cagagtcagt
     1981 atcttttgcc tgcttctttg atcattatcg gctgctggta cctgttattt cgatatcgac
     2041 atgtgctaga cgttttccat ctcggaaatg aacaagcagt aaacctcggg atagacacag
     2101 atcgttttga atttttgatt ttatgttgtg tcagtctttt gacagggaca tcaacggctc
     2161 ttgtgggtcc agttactttt ttagggttta ttgtcgcaaa tatcagttat cgtgtctttt
     2221 ctacttatcg tcatggtcag ctatttatcg gaagctttct ggtagctatg ctgtttctga
     2281 caggaggaca gctcgcagta gagcagattt tcggctggaa tacaacagtg agtgtcgtcg
     2341 ttgaatttgt cggaggaatt tattttgtca caaaattgat tcgcgaaagg aagagtatcc
     2401 tatgattgat ttacaaaagg tcaataaact gtaccatgga aaatcagtgg ttaaagatgt
     2461 acagttaacg attcctgaaa agaaattgac tgcttttatc ggtccaaatg gtgctggaaa
     2521 gtcaacggtg ttgtcgatga tcagtcgttt gataccaaaa gatacaggtg aaatctattt
     2581 agaccataac gaagtaaagg cttggaaatc caatgaatta gccaaaaaat tatcgatcct
     2641 acgacaatcg aatgaaataa atgtccaaat caccgtgaaa gaattagtag aatttggacg
     2701 atttccttat acgaaaggaa aattgacgac aaaagacgag gaaatcgttc aggaatcact
     2761 ggaacacctt gggttagaag aaatagcgga tcaatcgatt gacactcttt caggcggtca
     2821 actccaacgt gcgtatattg caatggtgct agcgcaagac acggattata ttttactgga
     2881 tgaacctttg aacaatctag atatgaattt tgcggttcaa atcatgcaga ttttaaaaaa
     2941 tttagtacaa gaaatcggga aaacaattgt tattgttctt catgatatca actttgctgc
     3001 aagttatgca gacgaaattg ttgcaatgaa agatggacag ttatttacgc aaggaacgac
     3061 ccaagaaatg ataaaaaaag atgtattgga tgaattatac gaaatggaca tacggatttg
     3121 tgagattgaa ggccgcagat tctgtcttta tttctaaaac taacattagg agagaagaga
     3181 tgaaaaaatt agtagtaggt atcttgattc tagcatcatt tggtttagca gcatgtggaa
     3241 atagcaacaa caccagtcag gcagacacaa aaaaaagcag cacacaaact gaaactacat
     3301 tgacgataac agatagtaat ggagaccaaa tcgaggtgcc aaataatcca gaaaaagtcg
     3361 tcgtattcga caatggttct ttagatacga tggatgctct tggtgttgga gataaggtag
     3421 ttggagcagc tacaagcagc cttcctgagt atctttcatc ctataaaaaa gtagaatcag
     3481 caggtggaat aaaagaacca gacttagaaa aaattaatca gcttcagcca gatctaatca
     3541 tcatttctgg ccgacaaaga gattttcaaa aagatctaag tcaaattgct ccgaccattt
     3601 tcctttcttt agatgcgaaa aatccatggg aatctttcca gcaaaatgtc actgctttag
     3661 gggagatctt tggtaagcaa gaagaagcta aaactcaatt agaggaatta tctagtgcaa
     3721 tcgatcagac gaaaaagaaa gcagaagcta ctgacaaaaa agcactagtc acacttgtga
     3781 atgaagggca attatcagcc tacggatctg gttctagatt cggtttcatc catgatttat
     3841 ttggctttga acaagcagat gatcaaatcg aagcatccac tcacggtcaa agtgtttctt
     3901 atgaatatgt gctggaaaaa aatccggata tcttatttgt agtagacaga acaaaagcta
     3961 ttggtggaga cgacagcaaa gatgatattt ctgccaatga actggtagca caaacgaatg
     4021 ctggtaaaaa tcagcagatc atctcccttg aaccagatgt ctggtattta agcgggggcg
     4081 gtttagaatc aatgaaactg atgatcgaag atgtgaatca agcatttaaa tagcataaat
     4141 aacagaggat taaactctca tactatgtaa caataatgtt tcttacattc ttaaatagca
     4201 agagcctatc ctcaaagaag ttatcaaaaa ccatccgtaa aatcgtattg atgattttac
     4261 ggatggtttt tatttatttg agaaatcttt tttcaacgtg aattcattat ctacatcata
     4321 cgggttgatt ctagaatggg gagtggcaaa aaatagtgtg atccattttt tcagcatcaa
     4381 gatcgcctcc tttgatttga atcaaaggta caaagcgaaa cttagataaa acttaaattt
     4441 agatattttt attttgtacc gatctcttct ttaaacgatg ttccatctgc tgcttctcgg
     4501 caataag
//
LOCUS       DACEOE010000179         4487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEOE010000179 DACEOE010000000
VERSION     DACEOE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.185"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            544..1107
                     /locus_tag="IW774_002394"
     CDS             544..1107
                     /locus_tag="IW774_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1154720.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IW774_002395"
     CDS             complement(1386..1673)
                     /locus_tag="IW774_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1154721.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IW774_002396"
     CDS             complement(1663..1992)
                     /locus_tag="IW774_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1154722.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IW774_002397"
     CDS             2297..2626
                     /locus_tag="IW774_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1154723.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW774_002398"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW774_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1154724.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IW774_002399"
     CDS             complement(3551..4462)
                     /locus_tag="IW774_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1154725.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1486 a          754 c          666 g         1581 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatata
//
LOCUS       DACEOE010000180         4428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEOE010000180 DACEOE010000000
VERSION     DACEOE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.186"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW774_002400"
     CDS             complement(543..2048)
                     /locus_tag="IW774_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1154726.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW774_002401"
     CDS             complement(2045..2731)
                     /locus_tag="IW774_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1154727.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW774_002402"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW774_002402"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1154728.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1237 a          919 c          729 g         1543 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaac
//
LOCUS       DACEOE010000181         4362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEOE010000181 DACEOE010000000
VERSION     DACEOE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.187"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            372..1220
                     /locus_tag="IW774_002403"
     CDS             372..1220
                     /locus_tag="IW774_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1154729.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1365..2306
                     /locus_tag="IW774_002404"
     CDS             1365..2306
                     /locus_tag="IW774_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1154730.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2287..4362
                     /locus_tag="IW774_002405"
                     /pseudo
     CDS             2287..4362
                     /locus_tag="IW774_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631314.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         1343 a          863 c          794 g         1362 t
ORIGIN      
        1 tagtttttgc catctcatcg atcatttctc acctgatttc taaacatatt gtttgatcaa
       61 aatgggttca ctcctaaaaa gataaaaaac tccctttcta tttatttaaa tacgcaaatc
      121 ttcctcatta ttttttattt tactcaattt aatcgtattc tcatttcaaa acgaatgtac
      181 tttaaaaaaa cataataaga tcactttgct tgtaggatgt attcccttcc attacaatga
      241 aataaatagc gattttatga gaaggtgatg tgaaaatgga agaaaaaata gaagaatcca
      301 aacaaaaaat cgatcagttg atataagaat tagaaaaagc ggtcatcggc aaacgttcaa
      361 ctatccaatt gatgatcgtg gccctattag caggtggtca tgtcttattc gaagatattc
      421 ccggtgtagg taaaacgtta ctggtcaaag cactagcaaa agctgtacag ggaaccttca
      481 gcagagtcca atgtactcct gatctgctgc ctagtgatat tcttggtttt tccgtataca
      541 attcacaatc aaaagagttt gaatttcgtc cgggaccgat ctttaccacg atcttgttag
      601 cagatgagat caatcgtacg actcctcgta ctcagtcagc acttttagaa gcaatggccg
      661 agaatcatgc aacgatcgac aacaatacat accctctaga caatcatttt tttgtcttag
      721 ctacgcaaaa tccaatcgag tacgaaggaa catatccttt gccggaagca cagctcgatc
      781 gttttttatt ccgactacaa atcggttatc cttcttttga tgacgaactt ttgctacttt
      841 tggataaatt cgaaaaagtg ttggataact taaatgaagt attgaacagt tatgaaatag
      901 aagaaataaa aacaaatgtg gcgaatgtct ttgtcagtcc cgaagtggca tcttatgcct
      961 tgcagttagt gtcagcaaca agaagtcatg ctgccatcca gcttggcatc agtccacgag
     1021 gaagcctttc ctttattcag gcagcaaaag cttatgcttt aatccatggg agaaattacg
     1081 tcacgcccaa agatttgcag gatttagttc cttacgtgtt ttcccatcgc ctgatttttt
     1141 acgatcgttc attgaaggaa gacgaaaaga aacaaatcat tcatactctt gttgcacaaa
     1201 ttcctattcc tgtgaggtag attgaggtag acaatggaaa aaatcaaaga ttatttcatt
     1261 aattttgtgt tatttctggt atatttcatc attgtgcttt ttgctcttgt attcaacaat
     1321 tctttaggct ggttcttgtt ggcctttttt actctttatg tatcatggag cttgatttca
     1381 ttaagcagct ttctaaagaa actttcggtt acttgtacat tgcagccact cgtttatcgc
     1441 aaaaaaagta tgcaggttat tttttccata gcaaaaaaga gaactttttc tttacctctc
     1501 cctcgtttac aaatcggctt cccagcaatc tttgcagaaa aaaacaagag attttgattc
     1561 ttacaaataa accgcaagaa gtccaattgc tttggcagcc aaaagaaaga ggatgccatg
     1621 aaacattaac tgtcattttt acagcctatg atagtctagg acttttcaga aaaagtgcaa
     1681 aaagagagat ctcttttcct gtgactgttc tgcctgcttt ttgtaagatt caagcagaaa
     1741 aattacagag agttctggaa aaaaaacagc agctgaacgc gtaccaaaga ggtcttgatt
     1801 ttcgggaaca tcgcgcttat cgtccaggtg attctttcaa ccgaatcgat tggaaattgt
     1861 ctgctcatac tcaagagtgg ttataccgtg aatacgatta tcaagaagaa gaatgttctc
     1921 ctcttttttg cttttggggt tcgcccagtc caaaatttga acctctgcta gacttgtatt
     1981 tttctttatg gcatttgaga aaagaaaagc agccggaact tctgatatta ggcaaccgcg
     2041 cctttcatgg caaagacccc ggtcatacct attttgcttc tttggaagct ggcgatgaga
     2101 aaactttttt cgcacacttg aaaaaatatg caaaaaaaca aatcgtcctc tttgttcctg
     2161 agaattctcc tgaagtctca gaagctattg aaacattgtc aaaagatcga acggtttatc
     2221 ttgtttattt cgtagaaaaa caactcatgg tccaagaaaa agacaagata tgttcattgc
     2281 caggaggtga atggattgac gattaaacgg gaaaaatggc tgcgtggcgt ttactccttt
     2341 gtgatactaa gtgtaggtgc tccgatattt gtagtagcta atcaattgca aaacagctac
     2401 cttcttatcc taactattgg tgtcctatgc cttattgtct gctgtattcc taaaaaatgg
     2461 gtacgtttgc tgctttcttt gccagtaatg ataggttgtt tgtttttata ttttccttcg
     2521 aagacatttt ccatattatg gatcttacag tttttttcgt cagttacaga ggaattcttc
     2581 catctatccc aaggtaaact ttattatttg ccagaaaaga cagctttttt tctcataatg
     2641 ttgctgatct atttatttat tacattgacc gtggattacg actattggta cgcacccttt
     2701 ctttctttga tggcttactt catgatgctt accgtttttc ggaaaaaaga tttactgtac
     2761 gaaatgattg cggtagtaac tgttttgttc gtgtatctgg cagcaaggca atttttctcc
     2821 atccggttgt tgtcgagaaa cactcgattc cagtcattga ctacagttct gcttatttgt
     2881 ttcttaactt ttagcgctat cttgccttta tatcttccga aagtacacca atctctcgag
     2941 gaaacgagtg aaccgatccg agaatatctt aatgatgaag ggctttatga cacactccat
     3001 gagtatcaat taaccggaag tgcaagaaca ggcttcagtg agaatgatga acagcttggt
     3061 ggacctttat atgataatgg cgaagttgtt tttacagcga accaaaaagg aaatggctat
     3121 tggaagatcg aaaataaaaa tcattacacc ggttctggat gaatagaaga tcaggacgca
     3181 cccatccaac caatccaaga attccctgtt gacatcacga gctaccaaac cgttatagaa
     3241 gacaaagaaa acattacatt gaccctaaaa aaacaagctt tcttacctta tccttatgga
     3301 aatactacct tgactgattt ttcattagct agtggacaaa cgctttacta tttgcctgaa
     3361 agccagcgat tcattcctgt cccgaatgaa tcgacacgtt ctgcaacttt acaaacagaa
     3421 aggttagttt ttacgagaga agaattaaaa agcagtcagt taaaagctga tgatttagaa
     3481 aatcagcaga caaatcttca attgccaaaa agtttgccta aacgtattga ggacttggcg
     3541 gtcgaattga ccaaaaatga agcaacttta attcgtaaag tcgaagctgt ctaacagtat
     3601 ttaaaaacaa gcggaaattt ccgctattcc aaaacggata ccgggcatac tccaaaagaa
     3661 cacgattatg tggattattt cctctttgat tcaaaagtcg gctattgtga caacttttca
     3721 acttctatgg tcgtccttct tcgaacactt ggtatcccag ctcgctggac aaaaggtttt
     3781 acaaatgggc tcagaacagg cacagataat caagggaatg aggaatacag cattctcaat
     3841 agtcatgctc attcttggcc agaggtatac tttcctggat ttggctggct tccatttgaa
     3901 ccaacccctt catttgccaa tccaatgggt tcacaaccag ttgctgaaac aaccgaaacc
     3961 gtccagcctg ctatcgaaca accaaatgaa agcgaaaata tggcagaaac tatttccagt
     4021 gagagatctg aggtcattcc atcagaagaa acaacactca caccagctta taaaatcgtc
     4081 ttattcagcc ttggagtgtg tttatccgtc ttgttgttct ttctattgtt attcagaaaa
     4141 tctctgcgct ggacaagtta tcttatctat cttcattttt ttgcttctac taatacgatt
     4201 gcatatcaaa aacttcttaa ccctttggaa acattggtac cgcgcaaacc cgcagaatct
     4261 ttgcaggaat atagcctgcg aatagaacaa gaattcaagc tagatctgcg tttcattcga
     4321 ttaactactc aatatgaagc agaaatatat ggacgacaag ca
//
LOCUS       DACEOE010000182         4329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEOE010000182 DACEOE010000000
VERSION     DACEOE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.188"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(506..847)
                     /locus_tag="IW774_002406"
     CDS             complement(506..847)
                     /locus_tag="IW774_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1154731.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
     gene            complement(837..1118)
                     /locus_tag="IW774_002407"
     CDS             complement(837..1118)
                     /locus_tag="IW774_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ1154732.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            complement(1489..1728)
                     /locus_tag="IW774_002408"
     CDS             complement(1489..1728)
                     /locus_tag="IW774_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154733.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(1748..1996)
                     /locus_tag="IW774_002409"
     CDS             complement(1748..1996)
                     /locus_tag="IW774_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154734.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFLWLGQ"
     gene            complement(2011..2577)
                     /locus_tag="IW774_002410"
     CDS             complement(2011..2577)
                     /locus_tag="IW774_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154735.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            complement(2578..3123)
                     /locus_tag="IW774_002411"
     CDS             complement(2578..3123)
                     /locus_tag="IW774_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154736.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3149..3916)
                     /locus_tag="IW774_002412"
     CDS             complement(3149..3916)
                     /locus_tag="IW774_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ1154737.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1218 a          918 c          542 g         1651 t
ORIGIN      
        1 gctacgcgac cgttcgttcg tcttttcctc cgtgggactc acccaccgca agcggttggt
       61 tgagttgtgg ctcctattca aaattcacta atctgttatt ttttatttct attcaagtag
      121 gtaacgttcg tttgcaagca aactctctct atgtaacttg ccagttacat cagcggtaac
      181 gctacgtcgc aagcgacttg ctctaatgaa actgccgttt cattctgcct gttcgttcgt
      241 agcaagctac ttcactcact gtatccgtct gaattattgt tatttattgc tattcaatat
      301 ccgtcagaca ttttatttat ttacatacat tgtatataaa atatatctta taattcgtat
      361 attactaaat atcactattt aacaattctt aacgttttct taatgtttat aatcacgtat
      421 atatttacgt atattttcat atacataaat acgtttatat aaacgtatat cttaatatac
      481 atataaacaa aaaagaaaag ccttgttaga cttttctttg taatttctcg ataaacctat
      541 ttagcttttt cgtatcacta gctgataaca taccaacctt tttgaatgat atgttctctc
      601 ggcttttggg aatagccttt aaagacccta cgtcaatcca tgattgcttt ttaagtcctg
      661 ctttttccca ttctgcaata ggaaaataca ccctctttat ttggtctgat tttttctcat
      721 attgacttgt gattccaaag aattctactc tatcttcttt atcactaacc accaaaatag
      781 gtcttctttt accaccattt ttatctgtaa atgcaacata gaccgttact atctcattag
      841 gcttcatcga accactcttc taacttttca tctgtatcta gaacatctac tggcatgtcc
      901 ttagttagct gttgcaatct gattccttgc ttctcctctt cacttagctt tatctctact
      961 ggaaactcac ctctagcgac tacttgtttg taaagaattg ttattgctgt tgtaggattt
     1021 aaacctagct cttccaatat catttctgcg ttctctttta aattcttgtc tattccgact
     1081 tggattctgc ttttttcagt tggatttttg accttcattt catccactcc ttttgagtat
     1141 aaaacactca ttatttctca aattgtccac atttatgatt atattatact cttaataaac
     1201 tacattgcaa gtaatctaat caaattaaga tgctatatca tacggtaatt gaacaaattg
     1261 tcgctaaccc acacatacgg aatcgtaatg aatgaaaaaa agacaagcct attcgcagta
     1321 ggcttgtctt tgtgcttctc ttgtacaaaa tcttcacatt tgtaaattaa ttttagcacc
     1381 tttgttagga aagaacaact taaatgagat gctaaagaat taatagttct caatctagca
     1441 tattcactta ataccatctt atcttcattc atggttcacc acctcatctt aattattttc
     1501 aaattggcaa atcaatttat tttgggttaa caaaactaat agctgttcac tatcaaaaag
     1561 aacctcacaa aattcttttt ctatcctctc ttctggcatg taataaccta tttgtttcaa
     1621 tatcttatct ggataacctt ttcgttgggt ttttctcaat aaatcttcat catttccaac
     1681 ataataataa gaacaccatt gcataccctt acttttaaat tttgtcatgt ttctatcttc
     1741 ctttcactta ttgacccaac cataaaaaaa tatattttgc taaatcttcc ttttctattc
     1801 catataaaac attctctaaa tccaatttat attcctcgtc aacaaggctt tctgtatcgt
     1861 catcataaag aacatatact gcataatttc tttcgtaaaa ggcattagca agacttctgc
     1921 taatacctac cattcctttc catgtataac caaaatcaac caaatcttca tgatctagaa
     1981 aatccattcc ttccatgctg attcacactc ctattctgct gttgtgtaat atgcaacgca
     2041 atcatctgcc cctactaacc acacacacca atcttcaatt tcatcatcgc ttctcttact
     2101 caattcctct ttggtacaaa aaactacttc accattttct ttttccatct cataatcttg
     2161 tagcatgtat tcaaaccaac ttctttcggt tggaaattct atctcgtggc aactctggca
     2221 aataaaatca ccatattcat tgattcggta gcaaccttca tcttctacaa attcatgatt
     2281 acaacaaaca catctagtac ctgttaggac ttccccatta atcgttgttc taacggtatc
     2341 ttctcttgct acgtgttcac tgctgacaag ccaatcaatg attttctttg ccaattcttc
     2401 atcagaatcg gcaaagcctt taaacgtgtt tctctttcct tgaaagtagt atttgcaact
     2461 tgcggtagtt tctatggttc cataactttt ttggaataat tcaatcattt caccatcaat
     2521 cactttttga cttttcgctt ctaaattagg gaactggtct tttctttttt caaacactta
     2581 tttcacgctc tcttcttcta ggtttattcc ataattttct agcaataatc tgtatccctt
     2641 aataaaatct ttcgtagcaa actcttttcc gctttcacaa aacttgttta gtcctatctg
     2701 ttgaccctca aaatcaaaca atcgcaatct ggttttatca tctagactaa atccattttc
     2761 atcgaacacc atctctaaat gagcatagcc atcattgtta tctgaaaact tcatcacatc
     2821 ttcaatcgtt tcttgataag gttcatagcg cccaccaaac cctgtttcat cataagcact
     2881 cactccagat tcataggtaa caaaattgat aactcctgta cttctatcaa agattccttt
     2941 tgctaattct tctcttgatt tatctcgtaa gtcgtatgct ttattgatta aataaattcc
     3001 ttgattcaat acgtccatca gcatatatcg accatacccc cactgataat gaatcgacac
     3061 gcttgctttc gttttgctgt taccatattt gactgtaatt aacgcttgtg ttctttcgcc
     3121 catcttgttt tcctcttttc tctaattttt ataaagaacc attttcttga aatgagtaat
     3181 agcttgtgtt tttacttgtt acgatgatat ggtctaacaa ttcaatacca attgtttttc
     3241 ctgcgttttt caaattcgta gtaaaacttt tgtcattttc acttggaaat tcatatccgc
     3301 tcggatgatt atgtgatact gcgatttttg ctgaattgtt tagcaaagct agttgaaaaa
     3361 tctctcttgg tatcgctact gattgattaa ttccaccttt gaaaacttcg ctataagcaa
     3421 tgacctcatt ctttgtattc agacacatca ctagcaaaga ttcactagca ttgtttccaa
     3481 ttaagttagt agcaaagttt gctagaccaa ccgagcttgt gactctataa aaacttctat
     3541 cttcactgat atatttttgt tcgattttca agacattctt aatcaatcct tcaccttcat
     3601 tctcggtctt gttttcaact tcaatagatt caagactgct atttcccata tcagaaccca
     3661 acaaagcatt tctgaaatcc aatacctttt caaagttatc agtcataacc atttcatagg
     3721 tgctattcca ttcattcaaa tatttctctt tgatgagaat gaacgctctc ggtttacttc
     3781 tgccaattct gatattttgt gtatcgtgaa aaatatagtt atcaagtaaa gtaccatcgt
     3841 acttttcata gttcccactg atatttgtta acttgtttac catttcttga ttaacttcta
     3901 aaattctatc tctcatgttg aacgcttctt tctgtttttg atttttggtt tttcaaaatt
     3961 cttttttgtt tggattgttt tagagggagg tggttttttc ttcatcgcta tttggttttg
     4021 cattttatgt ttcttccttt cgttcattta tttatctttt tcgcctgtct tgccttgtcg
     4081 ggagtgatta gaatgcgttc ttatctgccg gattaataca agtgtttaag taaattattt
     4141 tcgtaacgct tgcgtttaga aaaaaattta cgccacttgt attaatccaa agatgagata
     4201 agctctaaac acttctgaca aggcaagaca ggcgaaaaag ataatgggcg taaggaatta
     4261 taaatggcaa aaccaaataa agagcgatga atttcaatgc tttcaggcgt agcttgaaag
     4321 cctaaaagt
//
LOCUS       DACEOE010000183         4305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEOE010000183 DACEOE010000000
VERSION     DACEOE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.189"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            148..1137
                     /locus_tag="IW774_002413"
     CDS             148..1137
                     /locus_tag="IW774_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1154738.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="IW774_002414"
     CDS             1134..1553
                     /locus_tag="IW774_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154739.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="IW774_002415"
     CDS             1562..1870
                     /locus_tag="IW774_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154740.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="IW774_002416"
     CDS             1874..2236
                     /locus_tag="IW774_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154741.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="IW774_002417"
     CDS             2458..3864
                     /locus_tag="IW774_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1154742.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1497 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttta
     4021 aagggtgcgg ttttatgttt gcatgactaa gaatgagtgg gaaaatttaa cagccctatc
     4081 taacaggcgg ttaaatgtac aaaatatgta caccccctta aaaatgcagt ggtcgattga
     4141 tcgaatttta tgtctttggg ttttggggtt ttaaaacgaa aaaaaggggg cgattttcgc
     4201 cctctttttt gatgtttttt cttatcttga tactataggc aactatttct gcgaagaaat
     4261 cggggctata gtatgggtct atcccttgtg gctctaagga agaac
//
LOCUS       DACEOE010000184         4299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEOE010000184 DACEOE010000000
VERSION     DACEOE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.190"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..156
                     /locus_tag="IW774_002418"
     CDS             <1..156
                     /locus_tag="IW774_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ1154743.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IW774_002419"
     CDS             175..1452
                     /locus_tag="IW774_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1154744.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IW774_002420"
     CDS             1462..2118
                     /locus_tag="IW774_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154745.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IW774_002421"
     CDS             2118..3494
                     /locus_tag="IW774_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1154746.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3780..>4299)
                     /locus_tag="IW774_002422"
     CDS             complement(3780..>4299)
                     /locus_tag="IW774_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAQ1154747.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT         1681 a          505 c          750 g         1363 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatcgcag
     3961 actgtagaca aaagctgttc caaactcgta actcgagctt ggaacagctt tttatctact
     4021 tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc ctttccatag ccaggtggct
     4081 aatttcttta aattcattgc agcaaacact agcatcgtat gcgtcgcgac tttttccaag
     4141 cctcgatact tggtccaacg catcccatgc ttttcttttg catccgcaaa tattcgttca
     4201 atcgtttgtt tccgtttttt gtaaattgat ttattcagat ctgttaggcg aagatgttcc
     4261 acttcatcca tcagatctcc ccaaacatgt ctcgtgatg
//
LOCUS       DACEOE010000185         4282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEOE010000185 DACEOE010000000
VERSION     DACEOE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.191"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1468
                     /locus_tag="IW774_002423"
     CDS             <1..1468
                     /locus_tag="IW774_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1154748.1"
                     /translation="LKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQDSYKRFI
                     QPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTGCKLAVV
                     DATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRESELFVA
                     EQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQDPLAELV
                     KIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHISGLNKTT
                     AQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGIHPESYS
                     IAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLTQPGRDM
                     RDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHISKLSKKF
                     VKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            1488..1970
                     /locus_tag="IW774_002424"
     CDS             1488..1970
                     /locus_tag="IW774_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1154749.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            1986..2747
                     /locus_tag="IW774_002425"
     CDS             1986..2747
                     /locus_tag="IW774_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1154750.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            2878..4212
                     /locus_tag="IW774_002426"
     CDS             2878..4212
                     /locus_tag="IW774_002426"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1154751.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT         1432 a          776 c          927 g         1147 t
ORIGIN      
        1 tctgaaagta ttctttcaag tagatgaagc agcaatttta gcttacttgg atcgccaaat
       61 agtcaaaaat cctgcctctc cttcttcttc attcgtcaga gaagcctatc aggatagtta
      121 taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga ctgaaaaggc
      181 agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc
      241 actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc
      301 agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc
      361 agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca
      421 agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc
      481 tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc
      541 ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga
      601 aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat
      661 cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc
      721 tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac
      781 agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat
      841 tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc
      901 acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa
      961 aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt
     1021 aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac
     1081 acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga
     1141 tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt
     1201 attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac
     1261 ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact
     1321 tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt
     1381 aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt
     1441 gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag
     1501 aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc
     1561 aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc
     1621 atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca
     1681 tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag
     1741 atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga
     1801 cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc
     1861 gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat
     1921 ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa
     1981 ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt
     2041 tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt attttagctg
     2101 gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag
     2161 atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg
     2221 aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc
     2281 taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt
     2341 ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa
     2401 aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag
     2461 tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc
     2521 acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat
     2581 atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg
     2641 gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt
     2701 acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag
     2761 cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg
     2821 agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg
     2881 gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg
     2941 gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc
     3001 tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat
     3061 gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc
     3121 cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt
     3181 atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga
     3241 ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca
     3301 ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat
     3361 ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat
     3421 tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa
     3481 aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg
     3541 atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca
     3601 acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta
     3661 ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc
     3721 gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt
     3781 cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct
     3841 gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt
     3901 gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa
     3961 gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa
     4021 caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc
     4081 aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt
     4141 gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa
     4201 aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca
     4261 aaaatcttgc caagccccct tt
//
LOCUS       DACEOE010000186         4214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEOE010000186 DACEOE010000000
VERSION     DACEOE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.192"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(10..540)
                     /locus_tag="IW774_002427"
     CDS             complement(10..540)
                     /locus_tag="IW774_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1154752.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            645..1196
                     /locus_tag="IW774_002428"
     CDS             645..1196
                     /locus_tag="IW774_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1154753.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1349..1822)
                     /locus_tag="IW774_002429"
     CDS             complement(1349..1822)
                     /locus_tag="IW774_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1154754.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            1960..3432
                     /locus_tag="IW774_002430"
     CDS             1960..3432
                     /locus_tag="IW774_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1154755.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3429..4139
                     /locus_tag="IW774_002431"
     CDS             3429..4139
                     /locus_tag="IW774_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1154756.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1332 a          749 c          823 g         1310 t
ORIGIN      
        1 ttcacaacct tattttactc gtttataatt ttcatcgaag tattttccgc tacggatatc
       61 atctggcatt ccatcgtatg gttcttcgct aataacatct tcattgtcta tacctgcatc
      121 acccatataa gtgatcgtag caaattcagg aacaagtaat tttggataag tttcatattt
      181 ttcacgagct gcttccattt ctgcgatatg ttcgttttgg aaagtaacag tgatttctgg
      241 atgttcttca acccatttag ggaaaaagat cattccgtga agtttctttt catttggcat
      301 aaagtccaat gccacgtctg tccctgttgg ctctgtccaa gtgattttga atacgccgtc
      361 cgtcaattta acgatatcag cttcttggtc ttttacccaa cggcctttga ccattccgcc
      421 atggatacgg taatcaacag tatggtcatt tttggcatac cattcatatt cccaaccatt
      481 gtcatacgta tagataaagt gagttccaat aaaatcttcc aattctttaa attctttcat
      541 gtttaagcat ctccttataa attaaaacat gtaataaact acatgttttc gattactcat
      601 ttatgataga atggatttaa agatttgtca aggagggaaa actgatggca agaaaaaata
      661 cacttcgata tattttgtta gggttattaa gtaagaagaa gatgacagga tatgaattga
      721 atcaatcgtt caaaaatgaa ataggagaat tttggcaagc aaaacatagt cagatttatc
      781 ctgaacttgc aaaaatggaa gagcaaggaa tcattcagca tcaagtagag atcacaggtg
      841 aaaaattgga aaagaaagtt tatgaattaa cagaagaagg gaaggaacaa ttggcagagt
      901 ggattcatac acccaccaat gaactaccgg ttaatcgaga cgaatttgtt ctgaaattgt
      961 atttcgtcaa agacgtgaat gatccggcat tagtagaaat tattcagcag cagcgtgacc
     1021 ttcatgaaga aaaacgactt catctattag agcgaaaaaa aacgatcttt cctactgaaa
     1081 aagaaaaaca tgaaaattac ggacactacc tgattttagc gcatgcgatc aatcgggaaa
     1141 cagaatatgc aaattggcta acagatgtat tagaagaaag taaccgaaaa aaataaaaaa
     1201 atatccgaac agtgatggag aattcctttc tatatgaaag aaacgcagat caccgttcgg
     1261 atattcttat gttcaaacag gccttttttc aagtatttat gtagcatcta cttattaaaa
     1321 agcttggtac ttaggagatt agtggcgttt agagttcttt taacaactgt tcgtagtcag
     1381 ctttttcatc gacaggaagg agcttgtaag ttaattcgtc tgtctcgttg tcatgaatga
     1441 tgtacatctt agcattctct tctttgaagt taccgatttg taaaatcgta tagcgttcgt
     1501 cgatcaattc gatatctttg attgcttttg aaaaaacttt acggacatgg tgcattttct
     1561 tttggcggat ttctgccttt tctgcagctt ttgacaaagt aaatccgtca tctaaatatt
     1621 ttttgatcag ttctactttt atgactgtag gtaaacgata ttttctagga caattcgcat
     1681 cagcttcgat cgaacgaatg aatccctttt gttcccaata ccttaattga cgtggagaca
     1741 cacctacgat atcacttaat tcactgatgc caacaaccaa ttgatcattt tccaacaaac
     1801 gtttaaggga agccccaacc atttttgtca ctccttttgt cctcttgctt cccttaatta
     1861 tatccgtatt aacttatgtg tcaagtaggt tgacaaatag cgaatttagg actaaagtat
     1921 tctagtagtt tcagaaaaaa ttcagaaaga aggagaaata tgagtaacaa ccaacccgta
     1981 gatatttacg gaaaaccata taatcggaca cttttagttg ttgtcttatt aattggtaca
     2041 ttttgtaccg ttttgaatca gaccttattg acgacagctt ttcctacatt gatgaaagct
     2101 ttcgatattt cagcgtctga tgtgcaatgg ttaacgactg gtttcttgct agtcaacggg
     2161 atcatgatcc caataacagc atggctgatc aataaattca gttcaagaaa tttatatctt
     2221 tctgccatga cgatcttttt gatcggaacg attacttgct ttacagcacc taattttagt
     2281 acattactaa tcggtcgttt gatccaagct tgcggtgtgg gagtatcgat gccattattg
     2341 caaaatatca tgctatctat tttcccgcca gaaaaaagag gatcagcaat ggggatgtca
     2401 gggatcgtta ttggacttgc tcctgcttta ggccctacat tatctggtta tatcattgat
     2461 aactaccatt ggcgtgactt gtttggtatg gtactgccaa tcgttgttct tgtcttggtt
     2521 ttagcattct tcctaatgaa aagtgttatt ccattgtcaa atcctagcat tgatatttta
     2581 tccgctattt tatcaacgat tggattcggt agtttacttt acggattttc tagtgtagga
     2641 gatgacggct ggggaagctt gaaagtaatt ggtttcttag ttgttggtgt gattttcatc
     2701 gccttgttct catggcgtca attacatttg gaacatccat tcttggaact tcgtgtattc
     2761 aaatcatcta cattcacgat tgcagctgtt ttagctggtg taacaaatat ggcgatggta
     2821 ggggcagaaa tggtcgtacc attgtatatt caaaatattc gtggagaatc agcattccat
     2881 tctggcttga tgttgttgcc gggtgcattg atcatggggg ctatgatgcc tgtgacgggt
     2941 gcgattttcg ataaatacgg cgcgaaacgt ttagccatca cagggatgtt cattttgaca
     3001 gcggcaactg caccatttgc tttcttgaca aaaacgactc cagttttata tatcgtgatc
     3061 ctttatgcga tccgtatgtt tgggatctcc atggtcatga tgccggtaac gacttctggt
     3121 atgaatgcat tgccaaataa cttgatcagt catgggacag cggtaaataa tacattccgt
     3181 caaatcgcaa gctcgattgg tacagctatt ttgatcagtg tgttgacgaa tgtgacaaaa
     3241 gcaaatatcc caagcaaatc tgtattggat aatacgccat tagcttataa agatcaggca
     3301 atcaatgcca cactgtcagg ttaccatgcc gcgtttatcg tagcgatcat ctttgggatt
     3361 cttggcttta ttattgcctt tttattgaag aaaaaagaac ctgttctaat gaaagcgggg
     3421 gataatgcat gattattgga gttttgattg tcagcttgct tgcttttgct ttaacgaacg
     3481 tgttcgctaa aaaaacatgg caaacctttt tatctttgat tttcggacta atcttcgtcg
     3541 cttcactaag tttgatcgtc gctaatttgt ccaatcactt tggaatggaa aaagtaacgg
     3601 aaacaaaaac agaaaaaatt gtttccagtg ctgattccca aggagcagat atgcttttgt
     3661 ataaagcttt agggaatgga aaagaaaaag tgtatcttta ccgaacaaat gaaaaacaag
     3721 aaaaaccaaa agcaactggt acggataacg aaacaaataa agtagaaaag acagacggcg
     3781 atgctgaaaa agtgacgaaa actacttatt gggaatacaa aaacgacatg tataaattct
     3841 ggttcaatat tgctgataac aatcacgaat atgacagccg agtgaataca ttcaaaatcc
     3901 cagagacatg ggtagaatta agtacggacc aagcagccaa gttagctgaa ttagtgaaga
     3961 aacagcaaag tacgatggaa agtgaagcaa aagcttatgt tcaagacggt atggtaaaag
     4021 caatgactga aaatccaaaa atgagcaaag cagagcaaga acaacgtacg aaagccttag
     4081 ctgctgaatt ccaacaacaa gcattcgcaa aattagtgaa agaagcaaaa ggcgaataat
     4141 attttgaaca ccatgtagga ggttgtgaaa aagctgctct gcatcaaagc agtaagaaca
     4201 atcagataaa aata
//
LOCUS       DACEOE010000187         4200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEOE010000187 DACEOE010000000
VERSION     DACEOE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.193"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1223)
                     /locus_tag="IW774_002432"
     CDS             complement(3..1223)
                     /locus_tag="IW774_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1154757.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1848)
                     /locus_tag="IW774_002433"
     CDS             complement(1243..1848)
                     /locus_tag="IW774_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1154758.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="IW774_002434"
     CDS             complement(1897..2829)
                     /locus_tag="IW774_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1154759.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3254..4111)
                     /locus_tag="IW774_002435"
     CDS             complement(3254..4111)
                     /locus_tag="IW774_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1154760.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1191 a          849 c          692 g         1468 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggcttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgcttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta catgagtaat tagtttttat
     2881 ttttgttact gttttcacaa ccagatgata cgcctgctag atttatttgt caaccgattt
     2941 tatatatttt tttatataat aaggtaattt taatataaaa ttttatattg taaaaaaaca
     3001 aataagggaa acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt
     3061 ctatttgatg gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg
     3121 ctttgtaacg tattttacga gaattacgac tttattttat tactctatct tgctgccata
     3181 cccgtctatc cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt
     3241 attttttcct tcgttaatca atagtatcat tttttttcat atgatttaat tcatcaatta
     3301 attttttgtt tttattaatt ttagcaaaag ctagtgcatc ttgaatcaat tcgagcactt
     3361 ctgccttcga cttattttta gcctgtgcgt tcttcgctaa ttgggcagca gctcgtgcta
     3421 agtaataagt caaatggtta tccgaacaaa tatcgattgc gtaatttagt agtgcatcac
     3481 ttgtttccaa atcaccgtct tgcgcaaaaa aaacaccaca atgaaagacg atatttaaca
     3541 cccgccagat atcttctgtt gtacgaatcg gatacgtata aattttttca aacactcgat
     3601 tgaaataaac ttctgctttt tccaactctt tttctctagc atagaccatt ccaatacctg
     3661 tatatgccaa taaacgaaac aacgtgttgg ctgtttctgc catcaacaac tgatcaaaat
     3721 ggaacaacac atccgtaatt ggtgcattcg taaaaatcat ttcaaatcct tttagatagt
     3781 agtaacgtgc aatcagctca gactcgtcaa ttaccttggg atcaatggct gctaaaatct
     3841 gtgtcaattg ttcatattca ctcgtaatca gtgaaaactc tgctttttct agctgttcat
     3901 caagttgtgt attgatcacg ttcatcgaag aaaaaagttc aactaacgga agattgattc
     3961 gattacataa tttgattaaa atttttaacg aagggacttg accattgttc tcaaatttac
     4021 ttaatgttgc ttgtgtacaa attccttcag acacttcttt ttgagataag ttggcagcct
     4081 ttcttgccgt aataaatttt tcaatgttca ttattttctc tccaatcaga cctcacacga
     4141 aaatcatttc tgtctcgatt ttagcatgtt ttctttggga atgctttaat tggttaagat
//
LOCUS       DACEOE010000188         4109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEOE010000188 DACEOE010000000
VERSION     DACEOE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.194"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..532)
                     /locus_tag="IW774_002436"
     CDS             complement(<1..532)
                     /locus_tag="IW774_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154761.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKIS"
     gene            798..2498
                     /locus_tag="IW774_002437"
     CDS             798..2498
                     /locus_tag="IW774_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1154762.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2468..2686
                     /locus_tag="IW774_002438"
     CDS             2468..2686
                     /locus_tag="IW774_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154763.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2706..3596
                     /locus_tag="IW774_002439"
     CDS             2706..3596
                     /locus_tag="IW774_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1154764.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKTREQVQNIE"
     gene            3614..3898
                     /locus_tag="IW774_002440"
     CDS             3614..3898
                     /locus_tag="IW774_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154765.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1443 a          678 c          817 g         1171 t
ORIGIN      
        1 cactgatttt ttcaacaggt gtttcttttt tgaaaaaaga tttttttaca ttgatcttat
       61 ctgacaagat cgaacttaat ttactagcaa aaatcctata aaattcatct aaatagcgat
      121 aataaatccg attcagctga tcattaagat cgaagtatac aaaattattc tgtagtttat
      181 aaaaaaacgg cgaatgaaga tgtgttttca tatgaccaaa atataataat tctgaaattt
      241 ctaaaggtgt caattctttc agcatcgtta aatcagtaaa gtcgatccac ttcaattcat
      301 cgaatccacg cctgttacga gaagaggaaa agaaacgctg aatattttct gtaccacgga
      361 ttactttcag ccctgtatgg gattcatatt ctccagcaga agcagatgga tcaagcaaga
      421 gcaagttttg aggaaaatgc acgatactgt ttgcaaagtc agtagcagtc agcccttttg
      481 ttaaaacagc attacttgtg gtatcaatat gtacataaac tgactcagtc atcatgttcc
      541 tcctcttatt tcctcttaat ctcttttatt ttacaacatt tgcattaaaa aagcttgttt
      601 cttttatttt aaaattccat tacaaaatca cgttttcatt gaaaacactc tcattttcaa
      661 ttaatttttg tgatttttgt attttttatt taaaggatgt tttatgaatt gttttcttat
      721 ggaatctaca aagtcgaata cgagactcat gaccttttta tgacgtactg gtatacttga
      781 agaaaaagga gagcgcgatg gagataaaaa aagtttactt ttcacagaaa atgtcgctgt
      841 attcgcctat ccagcaagca aaacaacccc ttccattttt tcaaagtcat caagaaagta
      901 gagacgcttt ttggcgttct gcaaaaaagc aatgtgcttt taaagcagta tcgaaaaaat
      961 ataccgtcta ttttactatt gctaaaagta gaggagatgt tatttttaat aatctgttga
     1021 ttgaaggtga aacttttgaa tggaaaaaat tttttcggta tatggaagct tgcgctcgtt
     1081 tttttattaa agaaaactgt tgttttttat ttcaatctcc tctatcagaa gaatggttgc
     1141 gtatttttca taaaaacggc tatcaaggaa ccacacaatt aaataaaaag ctggtctatc
     1201 atacagcact tgttttaggt ggaggaggag cacacggtgc ttatcagatt ggtgtgtggc
     1261 aggcgctgaa ggaacatgat atcaactttg agattatcac aggtacctcg gtaggtgcgt
     1321 tgaacggcgc attgatttta caaggagata tgaaaaaagc tgtgaattta tggaaaaagc
     1381 tgtcgacaag acaagtgctt gcgttaccag aaatggctgc agttgaagac cttcgtgaac
     1441 gcttctatcg agaaagacgc caaatgacga aaacagcact aattgaaggc ggggtttctt
     1501 ctgctccatt ggaaaagatg gtcaaagaca atttggattt gtccttactc aaacataatc
     1561 ccaaaatacg tttatatacg gtgtctacta aactgccaga actagcggaa gtagtgactg
     1621 atatccaaca gacaaaaaca gaagaaatac cagattggat actcgcctct gcttcatttt
     1681 atcccgctat ggcttatcga aagatcggaa aaaataaata tgcagacggt gggtatcgca
     1741 acaaaatacc tattgatatt gcgattaaca aaggagcaac agaggcattt gtcgtagatg
     1801 tgcaagggcc aggcccagcg aaaaaaattc gtatgccaga catttttatc cattggaaat
     1861 gtcaaacatt gtggacacta ggcagctttc tgctttttga tagccaaagg aaccaattaa
     1921 atttacaatt aggctatctg gaaatgaaaa aacgattagg ctattactat gggaactggt
     1981 atacatttca ttctgttaaa cctgcaagaa catactggcg aggatttttg tcttacttaa
     2041 caaaagaaat tcagttagaa ttatcttttt tcaaaagcat aaaattttgg caaaaactgc
     2101 gtaatttata taagaacagg gtcgtaccag agacatgcgg attggcgatg ctagaacttt
     2161 tagcaaaaaa acatttttta ctgcccaatg aaatctatca agtagacatg atgattcaaa
     2221 tgatctgcca acaagatata aaaagtatgc cggatgattt attggcacaa attggtcaat
     2281 taagtactga agaatggcga aggtatcgaa aataccagca aaaaatacaa aatgagcgca
     2341 cgaaaactgt ttattttgat tatctgctac aaggaaaacg caaggatcgt ttgcagcatg
     2401 aattgcttaa gcaaccagtt gacacattac tgatcctata tttatattat ctcaaggagg
     2461 aacaaccatg gcacaagaat tttcatatga aatcgtagaa gaaatcgcta tattgtcaga
     2521 aaacaacaag gggtggcgta aagaactgaa cctagtcagc tggaatggtc gcccgccaaa
     2581 attcgatctt cgagattggg cgccagacca tgaaaaaatg gggaaaggcc tgacattgac
     2641 gaacgaagag tttgaacaac tgcaaaaagc aatagaaaat atgtaatgag gaaggaccct
     2701 gtcgaatgaa aagtatgact ggttttggca aagccatccg cgaaacaaaa gcgtatcagt
     2761 tagatattga aataaaaagt gtcaaccagc gttttctaga tgttcaaata cgaagtccta
     2821 aaatcctgaa ctttgttgaa aacgattttc gtcagcttat taaacagcag ttaagccgcg
     2881 gtcgaatcga agtatttgtg aatctagctt atattggcaa aaacgaaaaa cagatcgtca
     2941 tcaactggga attgattgat tcacttatca ctcaattgac agacggaatt caacaacgat
     3001 acggtagtca aaatcaagtg tcaattagtc ggatggcgga acgcttcgcg ctggacgaaa
     3061 acttcgtaac ggtagaagaa aaaccggaag agagcatgga agaattggca cagttagctc
     3121 tagatacatt gaaaagcgca cttgattcta ttgaggcgag tcgagaaaaa gaaggtcaag
     3181 ctttagcttt agtgttgaaa aagaatactg aagaattcaa agaagtacta aaaaaactcg
     3241 atagttttgt agaaatctat gaaaaggaat ttcaaataag gtatcaaaaa aagctggagg
     3301 aatttcttgg agcgaaagtc gatgagcaac ggttgttgac ggagctagcc atcttgttgg
     3361 aacgcggaga tattcacgag gaactggatc gtctggcgat ccatgtacaa aaactagatg
     3421 aacttatcga agcacaatgt cctgtaggtc gggaattaga tttcctgatc caagaaatga
     3481 atcgggaaat caacacgatc ggttccaaat caaatgcaat cgaaattaaa aatcaagtcg
     3541 tccagtctaa gacgatctta gaaaaaacca gagaacaagt acaaaatatt gaatgacgaa
     3601 agaggtagag ataatgaaat attatgacgt aacgttccac gaattaagtg gaaaaactgt
     3661 ggttaaacgg gatatccctt ctgaaaaaaa cggctttgac gtatggaaag atgcctgtgc
     3721 agactataat gagaacgaac tgtttatttt gatcaacgac ggtgcttacg tgacgatgaa
     3781 tcggaaattt attgttcgaa ttgacacaga agaggtagaa gatccgacag aaaaagcacg
     3841 ttctcgaaaa gatgagatca tgggtgtagt gaatactttg tccaatatgg gcttttagca
     3901 aggatgctga acaaagaaat acaataaata atcagaattg gatttcttcg cttagcttct
     3961 ttgctctagg aatacattta aaggttgtga caaaattcgt gtcacaatct ttttttattt
     4021 tgaataagtg atgttcaaaa accattttct cggtattaag ccaaatttat gcaaaaatgt
     4081 gagaaactat tttcacaaaa tttttcttc
//
LOCUS       DACEOE010000189         4074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEOE010000189 DACEOE010000000
VERSION     DACEOE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.195"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..1051)
                     /locus_tag="IW774_002441"
     CDS             complement(77..1051)
                     /locus_tag="IW774_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1154766.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2915)
                     /locus_tag="IW774_002442"
     CDS             complement(1500..2915)
                     /locus_tag="IW774_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1154767.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESISAEMRYISKQREK
                     NEREKALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLNSFEKLSDGSYRLSSADF
                     VDLYQKASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDVSA
                     VVAENKKLKEENRSLRRRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKT
                     LASQQNAPERNIGRSENEMEW"
     gene            complement(3137..3499)
                     /locus_tag="IW774_002443"
     CDS             complement(3137..3499)
                     /locus_tag="IW774_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154768.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVSLR"
     gene            complement(3503..3820)
                     /locus_tag="IW774_002444"
     CDS             complement(3503..3820)
                     /locus_tag="IW774_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154769.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQIGVIYRNKSKG
                     KEL"
BASE COUNT         1153 a          896 c          576 g         1449 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attctgcaaa ggtttctcgt aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca gcaaatttcg atatttatat tttgtctttc
      241 gattaatttg cccccaataa tcatgttctg ttgttaaagc agtaatcatc attttgtcag
      301 ctacacttgt atcagcaggc aagtaatgag gactagcaaa actatctaag ctttcataaa
      361 ccatagcatg ccaatcagtc gctttccctc gtcttaattg taattctaac cgccaccaac
      421 tcacgatttc ttttggcaca atttttcttt tcttctcttg ttccagtttt ttattataca
      481 tccgaatttg acgttcagaa gagcgagatc cccaatatgc cgtctctaat tttccattcc
      541 gtccataaat aggcttaaaa gaaactggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ctgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaaatttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccgttacctt
      841 catttaggcg cataagctgt tcaaaatcta aaatcaacac atcatgtgta gtgtgataat
      901 agatattctc tttaagattt ccaacaatgg taattcggtc gattgaccac tgaattttta
      961 ggggggtgtc cataaaaggg acacttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aatttagttc ctcctcaaag aaaaaagaga
     1081 ttggtggaaa gaattttcca gagttcacgt aactctaaaa aattcataac cagcaatctc
     1141 tagtcttgaa gaaacctgaa attattcgta taatgaattt gagttacgga gctagtgtga
     1201 ttagtttccg taaaggtcga gaacgtgtcc agcgttgctc gactttattt ttttgctctt
     1261 ttttcagttt atttatttga taactcaata atagcacttc tctttagcta tgcaaatatc
     1321 caaaaacata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaagtt
     1381 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacagaccg cctccggtac cgttgactgc accgtataac
     1501 taccactcca tctcattctc agaacgccct atattacgtt ctggggcatt ctgctgacta
     1561 gccaacgttt tctcttgatc taatattttt agctcttcag atattcccat atctttttct
     1621 acaagcccaa aacgaattga taaacgagtg attgattttt gaaatgcgtt tttcagttga
     1681 gttaaccgcc gacgcaaaga acgattttct tccttcagct ttttgttttc agcaacaacc
     1741 gctgacacat caaccttttg ttgatttttc aaaaaagaat tttcttgttc taacgccatg
     1801 tttttatcaa ataaagcgat atttttatct aatgtatttc caagcgattc attcgccata
     1861 tcagcatatt ttttgctgtt cgtagcatta gacgcttttt gatacaaatc tacaaaatca
     1921 gcactagata agcgataaga accatctgat aatttttcaa aactattgag tggcacagga
     1981 tctttttgaa atacaacagc cagctctttt tccaaatcga taatcgattt tttctcttct
     2041 tcaattttcg ccttctcaga agccaccaga gccttctcac gctcgttttt ttctctttgc
     2101 ttagaaatat acctcatttc cgcagaaatg ctctcacgct cttttacagc cgtttggcgc
     2161 aattcagaaa tttcactcat gatttctttg tattcgtgaa tatctttaat tttgttggta
     2221 ccgacaatat cccttttcca accgtaatca ttcattaatc gttctataga gtcaacttct
     2281 tgatttctaa aattattgaa aagcactcga ccattatctt tatcagcgtg tatcccttgt
     2341 tgaaccagtg ctttattaaa gctcggctgc ttttgaacac cccgtttata accttcagca
     2401 accggcacaa tattcaagtg caggtgcgga cttatttcgt cattatgaat aacagcatta
     2461 taaacagcca aattagggtt tctctcttga aactccttgt aatactgaac caaaatttta
     2521 ttggcaatca ctcgattttg ttcagcttgt tgtttcgata tgccagtaga agaaccatct
     2581 gctcgatttg tctggaaatc atctaaaatg cctatttgca caataaattc tcgttgaggt
     2641 tccaactttt tatccttgcg cactttcgct aaatagtcct cgatttttct atcggctctt
     2701 ttttgtttcg cattatattt ttctaccact tcgccaaaca attcctcata tttatctgct
     2761 agattcgtct gctctaaata aatattttca tccgatttcg tgaagtcaat atgcttatgc
     2821 caatcattgg ttttctggct atcagttaac tcacgattat tatgagaaat acttgtattc
     2881 ttcgttcctt ttttaaatga aatactaaac cccaagttaa aaccctcctt cgcgctgtcg
     2941 ctaataaaaa gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat
     3001 tgtgacatgg ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca
     3061 acgaaaaaag gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt
     3121 cacgcaaaat aatcgcttat cgtaaactca cgtcattaaa cattgacgta aatttactct
     3181 cactcatctg acttttttta ctcggtatca ctccgagtaa gccgtcagat tcattgggat
     3241 aatctcgttc gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt
     3301 cgtgttgctg atcgtcagaa attgacgata accaccgctc aaaatcatca aaagattcaa
     3361 atccaaaatg cttccgaagt gccttaccca tttcagcatt ttccttctca atcaatcgtt
     3421 ttttctgttg acgaagtttt gcctgttctt ttttcaattt ttcttcttgt gctttaatat
     3481 ctgctaaagt cattcccaag atttacagct ccttgccttt tgatttattt cgataaataa
     3541 caccaatttg atttaattgc ataacataag ttaaaatatt cagccaaaat tccttttcag
     3601 attcatcttc tgtttcggct attttaccgt aaacaaattt atcagtaatc aaagaagaat
     3661 ttgctaggcc tacattgatt gtagaattga gaacgtccat cattctctca agcatttcac
     3721 tataattatc ttcatccggc aaattcaaat aatcagctaa tcgttcatac atcaattcaa
     3781 ttctatcaac tgtctcttct cttaaatcaa ttttatccat gttacacctc atttccaaaa
     3841 ctgccaccat ttccgattct ttaattttcg atacacctaa ttcatcagca agatttttta
     3901 tggtcatctt ttctgacaaa tcagtcatta gcttctccac tactttcagc taccgcataa
     3961 atatatttaa cggcgtgctt agggataata atcggagagt caattttatt atttttcaaa
     4021 atcaattcat attgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACEOE010000190         4041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEOE010000190 DACEOE010000000
VERSION     DACEOE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.196"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..775
                     /locus_tag="IW774_002445"
     CDS             179..775
                     /locus_tag="IW774_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1154770.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IW774_002446"
     CDS             899..2698
                     /locus_tag="IW774_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1154771.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IW774_002447"
     CDS             complement(2976..3188)
                     /locus_tag="IW774_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154772.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1188 a          688 c          712 g         1453 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat a
//
LOCUS       DACEOE010000191         3894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEOE010000191 DACEOE010000000
VERSION     DACEOE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.197"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..895)
                     /locus_tag="IW774_002448"
     CDS             complement(<1..895)
                     /locus_tag="IW774_002448"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1154773.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQR"
     gene            complement(1007..1339)
                     /locus_tag="IW774_002449"
     CDS             complement(1007..1339)
                     /locus_tag="IW774_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1154774.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1487..2362
                     /locus_tag="IW774_002450"
     CDS             1487..2362
                     /locus_tag="IW774_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1154775.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(2444..3025)
                     /locus_tag="IW774_002451"
     CDS             complement(2444..3025)
                     /locus_tag="IW774_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1154776.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3031..>3894)
                     /locus_tag="IW774_002452"
     CDS             complement(3031..>3894)
                     /locus_tag="IW774_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1154777.1"
                     /translation="DEPLIQFALAELYFSVDRFKEAAVVYETLLASGINEISGISMQE
                     RLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQLKENQQAINYLEELREV
                     NPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELYHLASENAFRLHDRQRA
                     EELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQMEETANPYAEWNLAHAYN
                     ELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRAQELLTYYLTLEPGDME
                     ALSLLDDLTER"
BASE COUNT         1078 a          819 c          674 g         1323 t
ORIGIN      
        1 ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg ttagaacact
       61 tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg ctgatcaata
      121 atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca aaacgtaaaa
      181 gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt tcaataaatg
      241 catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc acatttatac
      301 gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag aaggtttctt
      361 cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt cgtaaggcat
      421 cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct tctagatctt
      481 gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac gcattgatcg
      541 taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca tgatctggtc
      601 tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat tgttcatcat
      661 tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt ggaaaaagct
      721 gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt ggctggttca
      781 gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca ttctctaatt
      841 ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat ttcatttttc
      901 caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg tgactgtttg
      961 acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac tcgtccttac
     1021 gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca aaaacttcag
     1081 ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca tcaccaagtt
     1141 cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca taagtgtggt
     1201 tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga gctaatggag
     1261 agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc atactgtata
     1321 aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac tttaacgcaa
     1381 atcctagata agagcacttg agaaaatcga atttccatgc tacatttagg aaggaatttc
     1441 taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg tcaaacgaaa
     1501 aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat ttggtcttgt
     1561 ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca ccttgatctt
     1621 aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca tcccattgat
     1681 tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc taggtacact
     1741 ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca atttacagca
     1801 tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa gcgggatcgt
     1861 ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt tagaacaaaa
     1921 ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct tacttgcttc
     1981 attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt atgttttcag
     2041 tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac ttgtcgtctc
     2101 caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag gtgtgacttt
     2161 cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata tggtcgttag
     2221 ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa aagctttcct
     2281 ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga agccacagac
     2341 aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga aagattccac
     2401 atccagctga cctttgtctt ttttagtact atactatttt taactaattg ttatgttcac
     2461 ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact tcatcagata
     2521 atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga gcataaagca
     2581 tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat tcagtatctt
     2641 cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata gatagatatt
     2701 ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt ttccaattca
     2761 aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc aaaaacaatt
     2821 tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc gcaaaattcg
     2881 ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga taattcaaga
     2941 tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt aaaaaattct
     3001 tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat ctaataatga
     3061 cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt cttgcgctct
     3121 tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat ctggttcatg
     3181 tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt cttctaattc
     3241 attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt ccatctgatt
     3301 gatcacttgt atcacatctt catatctttc atcatccaag taaagattac ttaaagtaag
     3361 taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt cttctgctcg
     3421 ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt cgacttggta
     3481 aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca tttcttcttc
     3541 ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa cttctcgaag
     3601 ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg taaaagctag
     3661 ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag gtacgatagc
     3721 tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca tcgagattcc
     3781 tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg cctctttgaa
     3841 acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt catc
//
LOCUS       DACEOE010000192         3886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEOE010000192 DACEOE010000000
VERSION     DACEOE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.198"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            720..1325
                     /locus_tag="IW774_002453"
     CDS             720..1325
                     /locus_tag="IW774_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HAQ1154778.1"
                     /translation="MILENKLGLTNQVELAKAEEKLSKQKAKELYDSGKINEIEVGTF
                     KGLSEIHDFLFSEIYDFAGKIRTVNIAKGNFRFAPVMYLEQSLNHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1341..1916
                     /locus_tag="IW774_002454"
     CDS             1341..1916
                     /locus_tag="IW774_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1154779.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKLFAKKNNPNFKEGRPKATITPKKRHAYELI
                     TSGKSYKEVEAITGFSRSTLFRIKKQIEASE"
     gene            2076..2279
                     /locus_tag="IW774_002455"
     CDS             2076..2279
                     /locus_tag="IW774_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1154780.1"
                     /translation="MKEQQLGYTLWGRPLYGLERRTAVSIDCACGELIQLNIQTKQYN
                     CKNCGNCYQFIDQDFQLLNEKNK"
     gene            2388..3713
                     /locus_tag="IW774_002456"
     CDS             2388..3713
                     /locus_tag="IW774_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1154781.1"
                     /translation="MLDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKNLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIAPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKI
                     SITDNTRELQDYCLFLFNKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            3706..>3886
                     /locus_tag="IW774_002457"
     CDS             3706..>3886
                     /locus_tag="IW774_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154782.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANQNLAKEQMRTE
                     EIAEILNASVIKNRSV"
BASE COUNT         1426 a          599 c          791 g         1070 t
ORIGIN      
        1 cagcaactta aacagaaata cggtaaaagt gaatccagga ttatgtgcat tgcgttagat
       61 ttattgattg cgcaggaaaa agcaggattc aacataccag cgctcaaaaa gtgacgtcac
      121 tttttgactt aaaaaataaa agtgatagca cttttatata gtaaaaagaa gaataaacgt
      181 taagggatag aaaaagagaa aagaacaaaa aaagagctaa cagtaccatt gttagctctt
      241 atgcagtaat gaaaaccaag cctttaaaaa aggagatgtt tcatcacaaa cacaaacaga
      301 aaaataaaaa aatcaaaaat ctaataatca taaattgcta ccgaagcacc ttttattata
      361 attaatcaaa tataaaatta caagtggttt ttttaattat tactaatact caaaataaaa
      421 acagggtatg ctactgaaaa ataaagaaac acctgatttt atgaacactt tgttttaaaa
      481 taagaactga aaaaatcact caaaataatt gagtgatttt cttagcgatt aaatgtttat
      541 agtttgtgcc gaataaaata aaaaattttt gccatttaaa aagaaaagga cagatggctt
      601 ctattgaaaa ctaactacgt gtcaaaaact taatagtttt tgacacatgg ttaaattcgc
      661 gatacaatag gatttgtagt gtttcataaa cttatgtcaa aaagttaagg ggattttcca
      721 tgattttaga aaataaattg ggtttaacga atcaggttga gttggccaag gctgaagaaa
      781 aactcagtaa gcaaaaagca aaagaattat atgattctgg aaaaataaat gaaatcgaag
      841 tcggcacgtt taaaggcttg tctgaaattc atgattttct cttttcagaa atttatgatt
      901 ttgcaggaaa aattcggaca gttaatattg caaaaggaaa ttttcgtttt gcgccggtca
      961 tgtacctaga acaatcttta aatcatattg atcagatgcc gcaaacttct ttcgaagaga
     1021 ttattaagaa atacgtggaa atgaatatcg ctcacccttt tcgcgaagga aacgggcgca
     1081 gtactagaat ttggctggat ctcattttaa aggaagaatt gcaaaaagtg gtcgattgga
     1141 acttgatcga taaagcagac tatctttctg caatggagcg cagtccaata aatgacttgg
     1201 aaatacgcta tctcatttcc aatgccctga ctgataaaat tgacgatcgt gagttatata
     1261 tgaaaggaat cgatgtcagc tacttctatg aaggatactc tgagtatacg atagatgatt
     1321 tgtaaaagca ggtgagttaa atgacaaaat atggctatgc acgtgtctca accgcttctc
     1381 aaagtacaaa ggaacagata caacagctac ttgagaacgg tgttgagaag aagaatattt
     1441 tttctgaaaa atttacaggt acgaaacgag atcggccaaa gtttaatcag ctgttggctg
     1501 gtctgaagcc tggagacgaa atcgttgtta cgaaacttga tcgattcgcc cgtaatacaa
     1561 gagaagcact agagatcatt gaacctttac tcgatgataa cgtcacgatt aaggtattga
     1621 atctagggac gatcgaaaat acatcgatgg gtcgaatggt tacacgtacg ttgttgagtg
     1681 tagctgaaat ggaacgtgat atgattgtag aacgtactca ggaagggaaa ttatttgcga
     1741 aaaagaataa tcctaatttt aaagagggac gtccgaaagc aacaatcaca ccaaagaaga
     1801 ggcatgcata tgaattgatt acttccggca aatcgtataa agaagtggaa gccataacgg
     1861 gtttcagtcg tagtacactt tttagaatta agaaacaaat agaagcgagt gagtagaaga
     1921 ttgataaaat taatataaaa atctctttta aatcaaataa atcgttgttt atacgagcat
     1981 tctaaggtca ggttcactca ttaagatgta tcattggtcg aacgaagcgt cataccagct
     2041 acgaatagac atatttttag ggaggaacga agtaaatgaa agaacaacaa ttaggctaca
     2101 ctctttgggg acgtccatta tacggattag aacgaagaac ggctgtatcg attgactgtg
     2161 cttgtggtga gttgattcaa ctcaatattc aaacgaaaca atacaattgt aaaaattgtg
     2221 ggaattgtta ccaattcatt gatcaagact tccaattgct aaacgaaaaa aacaaatagg
     2281 tttattatag tcagacaatt gtctggctat ttttctattt agagaagatc ctcgttgcaa
     2341 acacgaacgt atgtttgtat aatatgagag aagaggggga gaactttatg cttgattact
     2401 caaaagagcc agtcaatgac tactttctaa tcgatatgaa aagtttttac gcaagtgtcg
     2461 aatgtataga aagaaattta gatccattaa cgactgaatt agttgtcatg agtcgagcgg
     2521 ataatacagg atcagggttg attttagctt cttcaccagc tgcaaagaag aaatatggta
     2581 tttctaatgt cagcagacca cgggatctac cacagccttt ccctaaaaca ttacatgttg
     2641 ttccaccacg tatgaacctt tatattaagc gtaatatgca ggtaaataat attttcagaa
     2701 gatatgtggc tgatgaagat ctactgattt actcgatcga tgaatcaatc ttaaaagtga
     2761 cccgatcact gaatcttttt acgactgaag gaacacgaag tcagcgaagg aagaatctcg
     2821 ctcaaatgat ccaagaacgt attaaagaag agctaggatt gattgctaca gtaggtgttg
     2881 gcgataatcc tcttttggct aaactagcct tagacaatga agcgaagcat aatgaaggat
     2941 ttattgccga atggacgtat caaaatgttc ctgataaagt atggaatatt cctgaaatga
     3001 cagacttttg gggtattggt tctcgaatga aaaaaaggct caaccaaatg gggatcatga
     3061 gtatcaaaga tttagctaat tggaatccat acaccattaa gagccgttta ggtgtgattg
     3121 ggttacagct gtacttccat gcaaatggta ttgatcgaac tgacatagca atcgcaccag
     3181 aaccgacaaa ggaaaaatca tatggcaatt cacaagtgct accacgagat tatactagaa
     3241 gaaatgaaat cgaactggtc gtaaaagaaa tggctgagca agtagcaatc cgtattaggc
     3301 aacaccactg taaaacaggt tgtgttcatt tacacattgg aacgtcgatt ttagaaacga
     3361 gaccagggtt ttcacaccag atgaagatat ctatcacaga taatacaaga gagctacaag
     3421 actattgtct gttcttattt aataaatact atgagggaca agaagtccgc catgtcggaa
     3481 tcacttacag caagctagtt tatactgatt cgcttcagct ggatttgttt agtgatccac
     3541 aaaaacagat tgatgacgaa aaactagaca aaataataga taaaattagg cagaagtatg
     3601 gttttacgtc aattgtacat gcttcttcga tgttagaagg cgctcgtagt attactagaa
     3661 gcacgttggt tggtggccat gcaggcggcg caggggggct taaaaatgat tgactataac
     3721 gatcgaggac gattgaaatg ggcaggtttc tatttatcgg atcatactga agagatcgat
     3781 gcagataatg agcaacgtgc aaatcaaaat ttagcaaagg aacaaatgag gactgaagag
     3841 attgcggaaa tattgaacgc ttcagtcata aaaaatagat cagtat
//
LOCUS       DACEOE010000193         3856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEOE010000193 DACEOE010000000
VERSION     DACEOE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.199"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(121..486)
                     /locus_tag="IW774_002458"
     CDS             complement(121..486)
                     /locus_tag="IW774_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAQ1154783.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            complement(830..2482)
                     /locus_tag="IW774_002459"
     CDS             complement(830..2482)
                     /locus_tag="IW774_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1154784.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            complement(2542..3121)
                     /locus_tag="IW774_002460"
                     /pseudo
     CDS             complement(2542..3121)
                     /locus_tag="IW774_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
BASE COUNT         1263 a          869 c          624 g         1100 t
ORIGIN      
        1 caaagcagct cccaagaact cgtatgcttc catatcctgt atgaaacatc ataatagccc
       61 agatactaat aactcagctt gcaacaagca atcatttcat tcctattttt ctctatagtt
      121 ttacaattgg tatgaataag aaatagaact gaataattgt cgaaataaac caaagggacc
      181 cagtgactcc tcatatactg gtagaaaaat tttaaataac aaaacatgag acaaatatac
      241 ttttgcagca tttaaatctt gtgtcatata aggtagtaac actgataccg taattacgat
      301 aatatacgga atataaatct tgataaatcg tttctgtaaa aattcaccca atgtaatagg
      361 cttttttaaa tatgacagat atagcccaaa acctgagcaa aagaagaata tatgcactcc
      421 tgtatcacca atagatattg ctttgttcag tattgctggc aaatacgatt gctgaaaata
      481 gtacatcaat acaatagcga atattgaaaa cgccttctca aaactaataa tatcacgttt
      541 atttctttcc ccctcatttt attatttcaa tagttttatt catattctac tcctttcatc
      601 tctattgctg aatacattat taaaactcta atatctctta tttctccctt actgctttga
      661 tcggtgcttc atcaggctgc tgtgtctctg ttaacacttc tttgcctttt cgatccacac
      721 agtcggcccc tttacctcaa ttatgtaaac agaaccgtaa agcaggaaat ttttattaaa
      781 catagatgtt gctacttcgg acattgacac tcagatcttt ttcatagtat tacttaaaca
      841 tttcagccag ttcagcagcc ataacagctt ccattacttc ggcagcttcc ttgctttctg
      901 cgctaacgct gatataggtc ttcagcttcg gctcagtgcc ggaaggccga accactacag
      961 agcagttgcc ctccagcaga tatttgagaa catcagactt cggcaggcca ttcagtcccg
     1021 tgctgtaatc caaggtttca atgaccttct tacctccaat catatcaaga cctttatgga
     1081 attcaaccat aattttctgc atcttggtaa aacctgccgc accctcaaac tcaaaggaat
     1141 gcagggtgtt caaacagtag ccatacttag cataaatctc gttcagctta tcaaacagac
     1201 tgataccctg agtcttgtag taagagaaca tctcgcagat cagataagcg gcgttgacac
     1261 catccttgtc acggacatag ctgccggata gatagccgta ggattcctca aagccgaaaa
     1321 tatagctgtc caccttgcct gccttttcca gcataccaat ctgttcgccg atgaacttga
     1381 agccggtcaa cacatcgaca gtacgcacgc catagttgga agcgatccgc tcgcccagat
     1441 ccatagtcac gatggtcttg accatcacag ggtcagtggg catcttgccg tgcttaattc
     1501 tctgagagca tatgtagtcc agaagcaaca gaccagtttc gttaccggac agcaggacat
     1561 aatccccttc tgcattcttc acggcgatac ccacacggtc gcagtcaggg tcggtagcca
     1621 gcagcagatc agcattatac ttcttggcat actccatgcc cagagacata gcttcattaa
     1681 tttccggatt cggataagga caggtcggga aattaccgtc tggcagacgc tgctcttcga
     1741 caacggtcac attgctgtag cccatttctg ccagagtacg ggtaacaggc accagaccgg
     1801 taccattcaa aggagaatac acgatagcaa cattcttgtc cacttcttcg ccaaacagaa
     1861 ccgactggcc ctttacttct tcaatgaaag cagtcagaac ctctggctgg atagaagcga
     1921 ttctggaagc gtcgccgccc attttcacat ctgcaaaaat atccagcttt tcaatttcag
     1981 ccagaatctc agcggcagcc tcggtggtga tctggcagcc gcctgcgcca tagaccttat
     2041 atccgttata cttggaagga ttatgggatg cggtgatcat aacaccggca gaagccttca
     2101 gataacggat agcaaaggat acggtaggca caggcagcag ctccggccac agatgcacct
     2161 gaataccgtt tgcagcaaag atgtccgcgg caacctttgc aaatacatcg gacttgattc
     2221 gggaatcata gcccacgacg acagaagggg tttcataggt cttattcaga tagtctgaca
     2281 gtccctggct tgccttacct acggtgtgta cgttcatacg attagtgcct gcaccaatgg
     2341 tgccacgcag accaccggta ccaaaggaca gatttttata gaaggagtcc tcaatgtcct
     2401 tctcggaata tgaccgcagt tcctctacaa tttctgcatt ggcatttgta agccaatgat
     2461 aataatttgc attatgatcc atagaatctc tccaatcttt taaaattctg ctcatttatt
     2521 tttaaggtta ggatgaatat atcagctcaa ataatatata accccactaa tgccgccacc
     2581 caaagtatta taaaataaat ctgacaattg gaatgtctct atgtgtaaaa ataactgcaa
     2641 aaactctata agacacgaac ataaaaaaac aaattttata atctctgata aatttttcca
     2701 aaatcttttt ctgaagcctt ataccacatt actagaatta taaatggaat aaacagaata
     2761 acattttcaa aacattctgt agttaatcta atctcccctg tcgtttcatt cacagagtaa
     2821 atccaccaac taccaaacac attagacaaa ggatttagcc aaacatatct atccatcagt
     2881 gttctaaaaa gaatcatatt tgtaaaaaac acaagcaaaa acatttttct aaatgagctt
     2941 gattttctga aataccgaat ccagacaaga aaagattttt tcaaacctaa tcctgatttc
     3001 tcctcagcca tcgcaaacaa gtataaaaac atagccaaaa ctgcaattaa aatcgaaacc
     3061 caaatatttg atacaatgca gataacacat taaccaaaat agaagatatg attttcttca
     3121 taaactctcc tttcatcatg cgctaaatga gtaccatcaa gaaggtttcg taaaaggagg
     3181 tacccactct aacatgaata acctcggcca gcgaaaataa aactcaacag aattcctatc
     3241 tctatgctgc tataatttca acaaatttac atgtaccagt tactaatcta atataatttt
     3301 ttcagcaagg taaatttcac actacacgca tacacaaaaa ctgtagaaat taaaattatt
     3361 atcattccac aagtcatttc tattaatgaa tttgtaatcc ccaattttgg gaaaataaaa
     3421 tttaaagcag ctactatata cggatgtaaa caaaataata acgtactaat tcttcgcaga
     3481 tgtactgtta tttttataaa tgtactatct ttaggctcag tgatactgat acaactacca
     3541 gtgctaatgg aatggaaaat attaacaaat tattgacttc tcgcaaagct atttgattta
     3601 tgatgaaact ttcaccaact aataagcatc ctatagctaa aacagcacat aagataaaat
     3661 tacggtattt aaagacctta ttaagtatag tgtttttgtg gaaggctaag tgttgcccaa
     3721 taatgacgca tggtacacct ataaataatc cattacgagt tgttacaaac acgcttttat
     3781 aacaatatct aatatactcg atggaatcat tcccagataa tataaaccaa taagattcaa
     3841 atgctaaacc tactac
//
LOCUS       DACEOE010000194         3829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEOE010000194 DACEOE010000000
VERSION     DACEOE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.200"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..680
                     /locus_tag="IW774_002461"
     CDS             <1..680
                     /locus_tag="IW774_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1154785.1"
                     /translation="YLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLE
                     LLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRM
                     IFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFG
                     QVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKK
                     YYPGKGQ"
     gene            682..1626
                     /locus_tag="IW774_002462"
     CDS             682..1626
                     /locus_tag="IW774_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1154786.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1825..2469
                     /gene="pcp"
                     /locus_tag="IW774_002463"
     CDS             1825..2469
                     /gene="pcp"
                     /locus_tag="IW774_002463"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1154787.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2548..3825
                     /locus_tag="IW774_002464"
     CDS             2548..3825
                     /locus_tag="IW774_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154788.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1219 a          673 c          850 g         1087 t
ORIGIN      
        1 attatcttag ccttttaggc gtcttgattg ttattctcgg gtttgcattt aaattagact
       61 cgatattgat cgtaatggtt gctttagttg ttacagcttt gactggcggt cttggcattc
      121 aaggaatgtt agaactttta ggtacaagtt ttgtaaacaa tcgcgggatg gcgattttta
      181 tcatcattat gttggcaaca ggaacgcttg aaagaaatgg tctgaaagaa tcagcggctt
      241 ccttgatcaa gcgattcaag aaagtatctg ctggtatgat tgtagatatt tatgggatct
      301 ttcgaatgat ctttgcggct tttaacgtga gttttggtgg agttgctgga tttgttcgtc
      361 caatcatttt accgatggca ttggggacca tcgaatcaaa agggttagaa gtccatccta
      421 agcatgaaga agaactaaaa ggtatggctt cagctatgga aaatgtctgc tggttctttg
      481 gccaagtact attcatcgga ggagcagggg cgttgctagt ccaatcaaca ctgaaagatc
      541 taggatatga cgtaacattg ggacagcttg ctttagtgga agttccagtg gctatcacgg
      601 ctttagttgt ggcaagtgtc tacttctatc ttaaagaaaa gaaattagcg aagaaatatt
      661 atccaggaaa ggggcaataa gatggacttt tttacaaatc ctgatatttt attaggcgat
      721 aaattactgg agatccttta tatcgtgatg ggactcgtat tgatttacac tggagtaaga
      781 aatttaacag ataaaaccaa tcctcatcgt tatgggtcag cctttttctg gacggtctta
      841 ggggtagtta ttgcaggagg aagatggctt ccggcaatcg tgaacggtgg cttgatcttt
      901 gcgatgacca taccagcaat tgctaagaaa gtcagcaaag gagaaagccg gcttccatca
      961 aaagaatata tggaaaaaat gtcagacaaa ctaggaatga aaatttttat tccagctttg
     1021 agtatcggtg tctttgcgat cttgtttgct ctatttacca atttaggtgc tttagtcggc
     1081 gtgggcgtcg gtgtttttgc agccatgcta atcttgatgt tcttttctcg agataatcga
     1141 ccaagtacat ttttagatga tgcagcagat atgctgggca cagtagggcc gttaagcatg
     1201 ttgccaatgc ttttagcttc tctaggtgcg gtatttacaa gcgctggagt tgggacagtg
     1261 atttccaatg cagtaggtac gatcgttcca gaaggaaatg tgaacatcgg catcatcatt
     1321 tacgctcttg gcatggtgat atttacggta atcatgggga atgcatttgc ggccatcacc
     1381 gtgatgacgg ttgggatcgg tgcaccattc gtatttagtc atggagcgaa tccagcgtta
     1441 ataggaatgg ttgcattgac ttgcggtttt tgtgggaccc tgctgacacc gatggctgct
     1501 aacttcaata tcgtgcctgt agcgatgctg gagatgaaag acaaatacgg agtgatcaaa
     1561 aaccagttgt ttattgcgtt atttatgcta gttttccaaa ttgcttatat gattctattt
     1621 aaataataga agagaggggt tgtgacagaa taattgtcgc aacctcttct aacattgtag
     1681 tggaacacta tttttttata agaatatatt aagtttgatc tgttatgtta tttaaactat
     1741 tgtttccttt atttacgctg ttcaaggaaa atggtatgat aaaagataac catatgttaa
     1801 atcaatcgga cggaggaaca acagatgaaa gtactagtaa caggttttga tccatttggt
     1861 ggggacaaag tgaatccagc ctatgaagca gtaaaaaaaa tgccagatga aatcagcggt
     1921 gctgaaatca tcaaagtgga agtaccgacc gtattcgaaa aaagcagtca agtagtcaaa
     1981 gaagccatcc aacagcacca accagatgtc gtgatctgtg tcggacaagc aggtgggcgg
     2041 tcagcagtct cgttcgaacg tgtcgcaatc aatctagcag aagcaagaat ccctgataat
     2101 gaaggaaatc agccattcga tacggcttta gaagaaaacg gtccagccgc ttactttacc
     2161 tcattgccaa tcaaagcaat gacaaaaaat gtacaagatc atggtctacc agcgtacatc
     2221 tcttatacag caggaacatt tgtctgcaat gatatcatgt atcgtttgct tcatttgatc
     2281 gaaacacaat tcccggcaat tcgaggtggg ttcatccacg tgccattctc acccgatcaa
     2341 gtgatcgatc gtccagtggg tacgccttct atgtctttag aagacattgc ttcttctttg
     2401 acttatgcag ttgaagcagc agtagaaaat aaagaagata tttccggaaa tgccggaaca
     2461 actcactaat ttagcaagaa acactggctg gttgctaacg ttatgttagt agccagcttt
     2521 ttgtaattag aaaggaaaga aacagccgtg gaagaatggc aatccgtctt tgaagaatgg
     2581 tttccaaaag aaatcagcaa aagctatccc atcaaaattt ctaaacaata cacaagcagt
     2641 caacgttggg agatctatgc aaaattaacg aaaaaacagc gggaactagt cgataagcac
     2701 cgccgatatc taatcagctc tcgttttatg gaagaacatt atttagctgc aacagattgg
     2761 gtgttttctg acttcaagat caatccgttt tttcgaacaa aacgaagcca gcaaaaattg
     2821 tattgtgagt gtggtagaga attaaaagtg caatacatcg ttaaatcgcc aaaaaccgga
     2881 aagatcctaa aactaggcat caaccacttt gccgatcacc tgcatgtctc tccgacagtg
     2941 gcagcttcga tccatcaggg aatgacaaaa gttgatctgg ccttagacga attattatgg
     3001 cttaagcaga agaatatcga ttttcctgaa ggactatggc aaaaatactg ttttgtcttg
     3061 taccaaaatc gtcggatgaa acagccatat ttaccggata taaaattagc tcagcggctc
     3121 gcagaatttc gccaagtaga aatgccgatt tacatagcgg attatcaagc attggaaaat
     3181 gaaatcaaga aaataagtga acacatcaat gggcagtcaa aaaagcgaca gataaaaaaa
     3241 gaactatttg acgactttgc agaagaatta gtaaaggatg tagaagaatt cttgatcaac
     3301 tatcgcgcat ttttacgtaa agattggcaa tccatcgtat atgaagaagt gccagtacat
     3361 cctaatgctt attttgaaac atttatttcc gtgttgagaa agacaaaacg tcaacgaaca
     3421 ccagaagtca ctgctcagat ggaatatttt gcgaaaaacc aacgattcat ccaaccaaaa
     3481 atctatctat ttatttggaa gcaatattgt cactatggtt ttacagaagg gttcttcgac
     3541 agcattcctc ggatagtcag aaatggtttt ttaaaagtat tgcgtaaaga acgagaagct
     3601 atacaatctg ctgataagaa agaccgtaca gtctcaaaag aaaaatggca actagtagtg
     3661 aaagatatcc aatctggaaa tgtgcaagag acaatcgaca aatggaaagg aaaacattat
     3721 cgttttactg aagctcagaa acaagcgttg gaatattatc agaagttaga agaatcactg
     3781 cgtttcaatg atgaggcaag aaagtatctg aaggaattac tataaaaaa
//
LOCUS       DACEOE010000195         3817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEOE010000195 DACEOE010000000
VERSION     DACEOE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.201"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            352..1041
                     /locus_tag="IW774_002465"
     CDS             352..1041
                     /locus_tag="IW774_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1154789.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IW774_002466"
     CDS             1028..2236
                     /locus_tag="IW774_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1154790.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IW774_002467"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IW774_002467"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1154791.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1258 a          658 c          771 g         1130 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaag
//
LOCUS       DACEOE010000196         3802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEOE010000196 DACEOE010000000
VERSION     DACEOE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.202"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..832)
                     /locus_tag="IW774_002468"
     CDS             complement(<1..832)
                     /locus_tag="IW774_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1154792.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
     gene            1098..1442
                     /locus_tag="IW774_002469"
     CDS             1098..1442
                     /locus_tag="IW774_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1154793.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1443..1862
                     /locus_tag="IW774_002470"
     CDS             1443..1862
                     /locus_tag="IW774_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1154794.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1954..2304)
                     /locus_tag="IW774_002471"
     CDS             complement(1954..2304)
                     /locus_tag="IW774_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154795.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2270..3601)
                     /locus_tag="IW774_002472"
     CDS             complement(2270..3601)
                     /locus_tag="IW774_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1154796.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1348 a          682 c          707 g         1065 t
ORIGIN      
        1 ctactgaatt gaaatccata gatttcaata aaccatctct acgtaacaat ccgttagaat
       61 tctcgtttag gcctctttga gacggcgttc ctggatcagc gaaataaatg gcaatatcat
      121 tgacattact gatctgtttc caatttgaaa attcctttcc acaatcaaaa gtgatggatt
      181 tgaataggtt tttcggtaca gattcaaacc attgatttaa ttttttttca atatcaatcg
      241 cttgtctacc acacggtttc aatgtgatga taacttttga taatcgttca actaaggtaa
      301 ttacagcact tttatgtttc agacctacga tagtgtcacc ttcaaggtga ccaaactcat
      361 ttgagaattg gctataatcc ttttcacgtt catgaataga gcggcggaaa gtttgttttc
      421 cacgtctttc ttgatgtcca ttcggtttac gcttgccttt catcggtaag tcagaagaat
      481 caaatagccc ctttttgaac atacgataaa tggtacgagc agaacaacta ataggaaacg
      541 ctgcacgacc aacaatcaca tcgggtgtcc atccttgaac aacctttctt tgaatatatt
      601 ctgattgttc ctcgggtaaa acaagcggac gtctaccaca gtttgatttg tttttcttat
      661 actgttgata atattctaag gctgattttc cttgcttgaa gaacagatat actttatgga
      721 tcgtttgtct tgaacgattc aagcaatggg cagttttagc aacagattga ttcattttga
      781 aataagactc tattattaca agttcatccg ttgtaagatg ggtataggtc atttgtactc
      841 actccttata attctttggt cggaactatt ttgagtgtag cacaaatgac ttttttagtt
      901 gtctagctta attttacaat cggcgaataa aaaaagcgtt agcaactatg cttaaaaagc
      961 atatcgactg aataaactaa gcattggaca ggctgaaaga ctacatttaa aatgaaatgc
     1021 gaagaaacaa tagaaaataa agttgcctta gaaccatagt tgaatcacct aacagtcgtg
     1081 aagggagaat aagagaaatg gcagaaaaac aaaccgcagg acgagatcaa ttaggagatt
     1141 ttgcaccaaa atttgcagaa ttgaatgatg atgttttatt tggacaagta tggtccagag
     1201 aagaattatc tgctcatcag cgtagtatca tcaccatatc tgcattaatt tcaggaggaa
     1261 attttgaaca attgccagct catctgaaga ttggcaagca aaatggcatt actagtgaag
     1321 aaatagcaga aatcattacc catttagctt tttatgtagg atggccaaaa gcttggtcag
     1381 cttttagttt agcaaaagaa atctatttag aagaaaatag cgaagataag aaagaggcgt
     1441 aaatgacgac aaaacacgaa gaaatacaca atggcgtgat ttttccagca ggtgaaaaaa
     1501 acgaagctta cgctcaagta tttaccgggc aaagctatct gaatatgtta gtgaatgatc
     1561 ccgaagtttc tgttggtgta ggtaatgtaa cttttgaacc aggttgtcgc aatcactggc
     1621 atgtccataa agacggctat caaatcttac ttgtgaccgg aggagaaggc tggtatcagg
     1681 aagaaggaaa gccagcaaga cgattaatgg ctggagatgt cgtcgtaacg cacaaaggga
     1741 ttaaacattg gcatggtgcg acaaaagaca gttggtttga acatttggcg atcactgctg
     1801 gtaattcaga gtttttagaa cctgtaacag atgaagtgta tgaaggattg gaaaatcaat
     1861 aatgtcaagc ttgaacagct cctgaaagta gattttaatc gaactttcag gagctgcttt
     1921 tttgattcag tcttttataa atagaaaata gggttaataa taggaatgag aagcacgtcg
     1981 aaagatctta atattgataa tcggctgggc atacatgatt tccacgtcat caatcaaact
     2041 acctaacaga tgataatctt cccgatcatg tcggggatta cctaatagtt cttgataata
     2101 gatttccata ctagggtccc gaggattgtt cagcaaatca gcaagtacat ctaaatcttc
     2161 tggctgagtt tctgccgtac cctgtatctc tatgaaaagc ccttcctcag caaattctac
     2221 tccaatacgt atttcagtta tttcatggga atataaataa gtaagaagct catcgatgat
     2281 tctttttgat aattcttttt ccaagtgtct ttttttcctt tctgacagcg gtaaagttat
     2341 ctagtatcgg tacaaggata cctgcaacga aaccagttga aaaaccactg ttatatagat
     2401 ttagtcctcc gtgcaaagtg ctgacgttat ggacaagagc aatatgtagt acaccagcta
     2461 ttacaccgta aaaactgccg taaaaaccgc ttatgggcgc gagacctgtc ccaaacacag
     2521 ctgcaacaag catgccagta ctggtgatct catgaaaagt tccaaacaaa gaagcaataa
     2581 atattccgac tagtatcgga aaactgtttt ttaaatgaca accaaaagca ctgaaaccca
     2641 ccgcagataa aatagcaccg atgacaggtc catttaactg accgccaacc agcaaaacat
     2701 aagaagacag catcagacca actaagccta tgttgatcaa agtagcacca ataccagaat
     2761 tagcaatgaa atcagttgtc aattttccag atgaatcgaa aatttctcga atccccaaaa
     2821 gccgaaagga attataatag aaaccaattc cgactgtcag cgaaaaaaag ccaaaaataa
     2881 tccaaatgag aggaaagtga tattcattga aaaccaatgt attttcgacg attgaatagc
     2941 tgaataacct caaaacagca gtaaaagtca tcgcaatcaa cccagaggtg aatccaatat
     3001 tataaagtgt gaatccacga tggaaaacta aagtttgagc agctaagggc ggaagaagta
     3061 gaccaattgc tatgccagct aaattcccca ataaaatacc tatcagtaaa ggaaaacgca
     3121 taccaaaagt gatataacta acgaccggtg ataatgcgct accaaacagt ccgatcatga
     3181 tgtactgaga aaaaggttta ttgacaaata cagcgtatag atacacacct aaaatgattg
     3241 agatagaatt ataaaggttt ttgccaaaaa aagagaaacc ggtaactgtt agaatcgaag
     3301 caaataaggg accgtttaaa atcaccttgt gccgataagc taaaagcaca gaaagtaagg
     3361 tcaaaatccc tacatttatg aatgcgcttc cgaaaccgcc taaagcaaaa taatctgtca
     3421 aaagattgct tggcgaggta agaatagtta gcaatccact ccaattatca gtaggagtat
     3481 tagaaagcaa gccaataaaa agaaaaaaaa cacctgtaaa accaagataa aaatactttc
     3541 ttttctgtga aacgattcgt ttttgaggat gaatataaat aacgtcagtt tcgttttcca
     3601 ttacacacct tctttttcat tttctttaac tataacttga aatgaaagat ttggacaact
     3661 atcatttagg tcaaaactaa aaatgtaagg gaacccattg aatttaacca aattttttta
     3721 attttgcaaa aaaaagttaa agaagactga aaaaaaattt aaaatataac gttttgagta
     3781 aaatgtttgc aaaaaatgct aa
//
LOCUS       DACEOE010000197         3762 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEOE010000197 DACEOE010000000
VERSION     DACEOE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.203"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            302..577
                     /gene="rpsP"
                     /locus_tag="IW774_002473"
     CDS             302..577
                     /gene="rpsP"
                     /locus_tag="IW774_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1154797.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            589..840
                     /locus_tag="IW774_002474"
     CDS             589..840
                     /locus_tag="IW774_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1154798.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            965..1756
                     /locus_tag="IW774_002475"
     CDS             965..1756
                     /locus_tag="IW774_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154799.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1926..2447
                     /gene="rimM"
                     /locus_tag="IW774_002476"
     CDS             1926..2447
                     /gene="rimM"
                     /locus_tag="IW774_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1154800.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2437..3198
                     /gene="trmD"
                     /locus_tag="IW774_002477"
     CDS             2437..3198
                     /gene="trmD"
                     /locus_tag="IW774_002477"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1154801.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3334..3681
                     /gene="rplS"
                     /locus_tag="IW774_002478"
     CDS             3334..3681
                     /gene="rplS"
                     /locus_tag="IW774_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1154802.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
BASE COUNT         1313 a          677 c          754 g         1018 t
ORIGIN      
        1 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
       61 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
      121 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
      181 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
      241 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
      301 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
      361 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
      421 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
      481 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
      541 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
      601 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
      661 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
      721 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
      781 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
      841 ataaacgtgc tgtactcgaa tattcgagtg cagctttttt gtttttttta ttaattgaag
      901 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
      961 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
     1021 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
     1081 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
     1141 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
     1201 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
     1261 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
     1321 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
     1381 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
     1441 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
     1501 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
     1561 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
     1621 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
     1681 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
     1741 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
     1801 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
     1861 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
     1921 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
     1981 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
     2041 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
     2101 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
     2161 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
     2221 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
     2281 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
     2341 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
     2401 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
     2461 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
     2521 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
     2581 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
     2641 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
     2701 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
     2761 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
     2821 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
     2881 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
     2941 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
     3001 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
     3061 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
     3121 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
     3181 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
     3241 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
     3301 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
     3361 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
     3421 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
     3481 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
     3541 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
     3601 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
     3661 atcaaagaaa tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa
     3721 gggttctttt ttcttagcat gtaatttgaa caccctgatc ca
//
LOCUS       DACEOE010000198         3718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEOE010000198 DACEOE010000000
VERSION     DACEOE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.204"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(20..904)
                     /gene="rsmA"
                     /locus_tag="IW774_002479"
     CDS             complement(20..904)
                     /gene="rsmA"
                     /locus_tag="IW774_002479"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1154803.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="IW774_002480"
     CDS             complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="IW774_002480"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1154804.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1459..2235)
                     /locus_tag="IW774_002481"
     CDS             complement(1459..2235)
                     /locus_tag="IW774_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1154805.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2390..3712)
                     /locus_tag="IW774_002482"
     CDS             complement(2390..3712)
                     /locus_tag="IW774_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1154806.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
BASE COUNT         1045 a          833 c          646 g         1194 t
ORIGIN      
        1 tctgtcacaa cctctttttt tactgcatat tttctgacat cgcattgctt aatgcggcga
       61 attcttgtaa tgacaaagtc tccccacgac gttttggatc gattccagct gtttccaagc
      121 tttttgctaa ccatgctttt gtctggtcat ctttgccata cgaatgctgc aagttattcc
      181 aaagcgtttt tcttctttgc tgaaatgccg cttttgtcaa tcggaaaaat gctttttcgt
      241 ctgtcacttc gactgccggt atatctcttc ttgtcagttt caaaatcgct gaatctacat
      301 ttggctgagg gacaaatact gtttttggta cgatgaatgc taaactggct tccatataat
      361 attgaacagc aatcgataat gaaccataag ctttcgttcc tggttcagca gagatacgat
      421 cagctacttc tttttgcatc atgacgacta gttcatccac aaccaaatct gattcaagaa
      481 aatgcatcat gatcggtgtc gtaatatagt aaggtaaatt cgctacgact tttaacggaa
      541 gctcctcatg aaatgtttct ctaacagctg ttgacaaatc ggtctttaaa atatcttgat
      601 gtacaatagt cacattgtca tagggttgca tcgtatcagc caatacaggg atcaaacgat
      661 catcgatctc aaatgctacg acttgttttg catgcttggc taattgttcc gtcaatgcac
      721 cgattcctgg tccaacttcg atcacgttcg tatgttcatc tatttgcgca gtttctacga
      781 ttttccttaa aatatttggt tcagtcagaa aattttgtcc taaacttttt ttaaatgaaa
      841 aaccatgttt caataaaatc tcttttgttc tagagggtgt cgcaatttca cgatattcac
      901 tcatgttgtt cctccactat tttcattgct tgggcgagtt ctttcggcgt aatacgaaac
      961 atcttcaaac gtttttccaa ttgtttgccg ttcgtgtaac cgatgcgcaa ctcgtcacct
     1021 agtttttctc gtttttcttt tgcttgtgcc ccagctatta aaccatagtc taaaaggtct
     1081 tgtctagtga tttcagaaac agccgtttgg gaaatgtctg gcgtcacgac ctttttcaat
     1141 gcttctaaaa tagcagcatc acttgcatgc tcgacaccaa ggcttccctt tttctttggt
     1201 ttagcttgtg tgcgcggcaa aaaagcatgt tttgcctgag gaacagcttc cataatgatt
     1261 tttcttattt tttctcctga aaaatcagga tcggtaaaaa cgatcactcc acgagtctct
     1321 tgggcatgtt caatttgcat caggatctct tcattgattg cagaccctat tgtttctatc
     1381 gtatctgcat caactacttc ccgcagcctt cttgtatcgt cttttccttc aacgactata
     1441 atctcttgga tcttcatctt atccatgttc gcttagacca aataatcggt gcgcatttgt
     1501 ccatgtttgg gcagccactt cttctgtcgg aagattccga agttcagcaa tcttttctac
     1561 tgtataacga gtatagcctg gttcatttcg ctttcctcta aaaggaactg gtgccagata
     1621 aggggcatct gtttccacaa gtaatcgttc gattggtaca tttgctgcag cctcttggac
     1681 gtctaatgct tttttgaaag tgacgacacc gctaaatgaa atatgcatcc ctaagtcaag
     1741 aaatcgtttt gcccattcat aatcaccact gaaactatgc atgattccac cgatatctcg
     1801 aacatcttct tcttttagga tttgatacgt gtcttcaagt gcttctctag tatgaatact
     1861 gattggtagg ttcatttctt ttgcgatagc aatctgtctg cgaaatactt tatcttggac
     1921 ttctttcggg tcttccatcc aatgataatc caagccaatt tcccctaatg ctacaacctt
     1981 tggtaaagtc aattgttctt gtaatttatt ttcgatttct ttcgtataac tgccagcctc
     2041 tgtcggatgc cagccgataa tactgtatat atttggatag tcatggctta attgcaaaga
     2101 cttttcaatc gttggcgtat cgaagcccac gaccgccatt ttcgttaccc ccatttcttg
     2161 tgctcgctga atcgtttctg gaatatcttc attgaactgt tcagcattta aatgggtatg
     2221 agaatcaaat atcatgttta tctctccttt tcattcttac tttagcacat aagcactact
     2281 tgcagaagta gcaaaaatca aaatagaaga acttgtttta ttgaaataga aaaagcacga
     2341 agatctagag gacctagttc gtactttttc tgtctgttgt tgaaatattt tactcttctg
     2401 agcgtttttt ctttttttga taatagactg ggatacctat caaggtaaca cctataccga
     2461 tcaatgctaa tttcgtttgc gtcatcatcg tcatacctaa aatgaaaata gcaccgagaa
     2521 tcgcaataat cggaacgatt ggataaagtg gtactttata tggacgtggc aattccggcg
     2581 ctttttttct taaaataaag acaccgataa aaatcagtaa actgaatagc cacatcacaa
     2641 aaatcaacat gtctgttaaa aagtcgaaag aaccaaggct catcataata caagctactg
     2701 ctaactggaa aaccgcagga acataaggaa ccgtaaactt ctttgaaagc tttgtcagct
     2761 gtttactgaa aggtagctcg tcttctaaag ccatcgcata aggcacgcgg atcccagtta
     2821 gtgtataacc gttcaatgca ccgtataccg agatcaagat cccaatagtg accagctttc
     2881 caccgattcc tccaaaaatg acgtctgatg cttcagaagc tgcattcaaa ttaccggcaa
     2941 gatgatcaat cggcaaagtt ttcaaaaaaa caaaattgat caatagataa acgactgtta
     3001 caaaactcaa accaagaata attgctttag gcaaatcttt ttccggccgt ttcatttccc
     3061 ctgccattgc accgacacct aaccagccgt cataagcaaa caacgttgct aacaacgcac
     3121 tgctcaatcc ttcgacaaaa gtaacatctt ttccagcttc gattggaaat aactggacag
     3181 ttccttgtcc tggggtcaat aatccccata tgacaatcac agcaattggt attaatttaa
     3241 caatcaacgt cactgattgt acagaagcac cgacttttgt ccctaacaaa ttcaaccctg
     3301 tcacagaaac agcggtgatc actgcaatca acaaaagata atcggtagat aattgtaata
     3361 agttcgtcaa ttgtgtcgca aagataatcg ataatgcagc aatattagca ggaaagtaaa
     3421 tcaagctttg cgcccatcct aataaaaagc tgggcaattt tccatacgca gcttcaatat
     3481 attttacagc tccaccagtt tccgggattg ctgtagctaa ttcagccact gtcaatccag
     3541 cacagatcgt taagaaacca cccaaaagcc aagctagcaa tgtcaaactt gtagactgag
     3601 tactagcagt aacagcagca gttttgaaga agacgcctgc accgatcacg gttcccataa
     3661 cggtagctaa tgcgccaaac agattgattt ctcgttttag tttgcctttc attcccat
//
LOCUS       DACEOE010000199         3697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEOE010000199 DACEOE010000000
VERSION     DACEOE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.205"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            123..1097
                     /locus_tag="IW774_002483"
     CDS             123..1097
                     /locus_tag="IW774_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1154807.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1079..1540
                     /locus_tag="IW774_002484"
     CDS             1079..1540
                     /locus_tag="IW774_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1154808.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1590..2054
                     /locus_tag="IW774_002485"
     CDS             1590..2054
                     /locus_tag="IW774_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1154809.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2068..2817
                     /locus_tag="IW774_002486"
     CDS             2068..2817
                     /locus_tag="IW774_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1154810.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2817..3647
                     /locus_tag="IW774_002487"
     CDS             2817..3647
                     /locus_tag="IW774_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1154811.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT         1245 a          489 c          714 g         1249 t
ORIGIN      
        1 ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa gcaatttcat
       61 agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg agggataaag
      121 aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta aaggaaaaga
      181 caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta atggctggtc
      241 aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct aacgaattta
      301 actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt tcccattctg
      361 gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga gcaatgacta
      421 tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat cctgttcact
      481 atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt tatggtttat
      541 tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc ttgaatgaat
      601 ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata actgctaaag
      661 aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc ggtattaatt
      721 acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg attaattcta
      781 atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat tatgatgtac
      841 cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg gtagaagact
      901 tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt gacttagaat
      961 ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt gttattcgcc
     1021 aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga cgctatatgt
     1081 ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat tatttcacat
     1141 tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa aattaatttt
     1201 aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta gtccagatga
     1261 ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc taattatttt
     1321 aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga acaacaatag
     1381 aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa tggcccagtc
     1441 agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact ctcaaaaaag
     1501 tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt tctattctat
     1561 aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta gaccacagat
     1621 tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac catattattg
     1681 tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca attgctaaac
     1741 ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt atggaaacat
     1801 ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt gaattagttc
     1861 aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa agcgagtcca
     1921 aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca aaaaaaattc
     1981 ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt gaaaatgtag
     2041 attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt aggtttagtt
     2101 ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg tagaccacta
     2161 attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat tgttcttggg
     2221 gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg acctcctaat
     2281 atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag tagtacagaa
     2341 acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag tattatcatg
     2401 cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa taataaattt
     2461 agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt tgtttacttt
     2521 attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt agtagataaa
     2581 attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc cgcattaggg
     2641 tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt tatttttgga
     2701 ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt tgcagctctg
     2761 attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca tggttaatgg
     2821 aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag atgtgtcttc
     2881 gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg ggatttatgt
     2941 atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa aaaagagaag
     3001 cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca tttattatgg
     3061 gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac tttaatactg
     3121 aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt ggtgattcat
     3181 ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt gctaagcaag
     3241 gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct attttagctg
     3301 ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact aaattgtatc
     3361 aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg gctgtagtag
     3421 gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact acagggggaa
     3481 cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta ccacttgtat
     3541 gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg ctcttattag
     3601 gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga ttaaggctct
     3661 aatttttgat atggacggag ttttaattga tagcgag
//
LOCUS       DACEOE010000200         3678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEOE010000200 DACEOE010000000
VERSION     DACEOE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.206"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..473)
                     /locus_tag="IW774_002488"
     CDS             complement(<1..473)
                     /locus_tag="IW774_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1154812.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTY"
     gene            complement(614..1216)
                     /locus_tag="IW774_002489"
     CDS             complement(614..1216)
                     /locus_tag="IW774_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1154813.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            1461..1667
                     /locus_tag="IW774_002490"
     CDS             1461..1667
                     /locus_tag="IW774_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154814.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            1979..2698
                     /locus_tag="IW774_002491"
     CDS             1979..2698
                     /locus_tag="IW774_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1154815.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(2779..3462)
                     /locus_tag="IW774_002492"
     CDS             complement(2779..3462)
                     /locus_tag="IW774_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1154816.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
BASE COUNT         1368 a          619 c          677 g         1014 t
ORIGIN      
        1 tagtatgtaa ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga
       61 aaccaagtct ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat
      121 aagacaatgt gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca
      181 aaaaaatcgc cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga
      241 agcgcgtaaa ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt
      301 cctatagcaa ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata
      361 caagaaatca gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat
      421 aagatacaaa ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac
      481 agctcccatt gatttttacc aaattatagc acaattggcg tattattttt ttaatttttc
      541 agggattttg aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa
      601 gttagttaat tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt
      661 agtttgtaat aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag
      721 aaaagtaaga agctgagaaa aataacgtaa tgagagaaaa aattagaaac atgctgatcg
      781 ccttctttaa taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg
      841 atccatagcg aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg
      901 agatagtcaa ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc
      961 acagcagaca aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga
     1021 tataagaaag aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta
     1081 gaacgaccgc tagttttcca agattattca ataaaacttc taagacaaat tttacttgga
     1141 catacaccat atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca
     1201 caactttatc tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa
     1261 aaacgtcttt tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa
     1321 aaatcttcgt tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact
     1381 ttttatgaca agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttgtaa
     1441 aaggtgatgg agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg
     1501 catttaaatc tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa
     1561 atagtgtgat gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc
     1621 tgcactggca gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat
     1681 gttgagagca taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt
     1741 taaaaaacag cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga
     1801 atactgttta tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg
     1861 aataggagtt acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa
     1921 ggcttttatt tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat
     1981 gccgataatt gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga
     2041 aatgcttgcc caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt
     2101 attgccgcta ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt
     2161 gaacaaacgc tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg
     2221 ctccatctac gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga
     2281 agtagaagta aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat
     2341 ttcgccacaa aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt
     2401 agcgagaatc aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta
     2461 ttcctactat aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg
     2521 tcccaaaata gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga
     2581 acaaatcgta caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca
     2641 gtcgatccgc tacactacaa ataacccgaa agcaaaggga atctttacaa ctaaataata
     2701 taaaaactaa gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt
     2761 ttttttgatt cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg
     2821 aaatgtctga gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc
     2881 gccaaacaga tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac
     2941 acttagatag tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata
     3001 taagttcgtt gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc
     3061 gttgatttta tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac
     3121 taatgcggga tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata
     3181 atatagaaca aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata
     3241 ctttgttaga agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact
     3301 tagccattct ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga
     3361 ggcaaaagca ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag
     3421 caaaagtcct attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta
     3481 tgaatatttt tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag
     3541 aaaactaaaa tcatatcgct tcccattttc atttttagaa aggtctgtta gtataaagaa
     3601 aaaatagaca acgaaacttt aaataaccaa cttgaaataa acaaattcaa agaagaaaga
     3661 tgggaaggaa atcaatca
//
LOCUS       DACEOE010000201         3598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEOE010000201 DACEOE010000000
VERSION     DACEOE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.207"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            150..1523
                     /gene="glmU"
                     /locus_tag="IW774_002493"
     CDS             150..1523
                     /gene="glmU"
                     /locus_tag="IW774_002493"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1154817.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            1617..2588
                     /locus_tag="IW774_002494"
     CDS             1617..2588
                     /locus_tag="IW774_002494"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1154818.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      2594..2696
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2762..3331
                     /gene="thiT"
                     /locus_tag="IW774_002495"
     CDS             2762..3331
                     /gene="thiT"
                     /locus_tag="IW774_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1154819.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(3412..>3598)
                     /locus_tag="IW774_002496"
     CDS             complement(3412..>3598)
                     /locus_tag="IW774_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154820.1"
                     /translation="DQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIATLIYSVTD
                     RHWQLVEDADLSYLPLK"
BASE COUNT         1178 a          644 c          797 g          979 t
ORIGIN      
        1 caccggttac caaatagata ggcccgttat ggtctatcta tttttttatc cagaaaacgc
       61 tgggataaaa gaaaatcact tgttttgttg aaaaaatggg tatactggga taaactgaaa
      121 tcagcaaact atatttagga gtgacgcagt tggacgcacg ttatgcaatt attttggcgg
      181 cagggaaagg tacccgcatg aagtcaaaat tatataaagt tttacatccc gtttctggta
      241 aacctatggt agaacatatc attaatcggg tcagtgaaac gaaaccagat gaagtgatta
      301 cgatcgttgg acacggagca gaacaagtta aagcacaact aggagaacgc agtaaatatg
      361 cgttacaagc agaacaattg ggaacaggac atgctgtttt gcaagctgct tctttcttgg
      421 aaggaaaaaa aggaacaacg ttagttatca gtggcgatac accattattg actactgaaa
      481 cgctgaacaa tctttttgaa tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg
      541 cacaagcaga aaacccaaca ggatatggac gaatcatccg tgaccatatt gggatcgtag
      601 aaaaaatcgt ggaacaaaaa gatgcaacac cagaagaagc tcttgtacaa gaaatcaata
      661 caggaactta ctgttttgac aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa
      721 acaatgctca aggtgaatac tatctaacag atatcatcga aatcttaaaa gaagaaggcc
      781 atacagttgc tgcttatcaa acggatgatt ttgaagaatc aatgggagtc aatgatcgga
      841 tcgcgttagc taaagcaaat gagatcatga gaaagcgaat taaccagatg catatggtca
      901 atggcgtaag ctttgtcgat tcagcaacga catacatcga tgcaggcgta gaaatcggtc
      961 cagacacatt gatcgaagca ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt
     1021 gtgtcatcgg gtcacattca aaaatcgtag acagtaggat tgaggatcac gtcgtcattg
     1081 aaaactcagt cattgaaagc agccatgtca agaaacatgc agatgtggga ccttatgcac
     1141 atttacgacc aaaagctgaa attggcgaaa acgtccatat cggaaacttt gtggaagtga
     1201 aaaatgctca aatcggcaaa ggaactaaag tcggtcactt gacttatgtt ggtgatgcta
     1261 cgctgggaga agaaatcaat gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga
     1321 acaaacatca tacgactgtc ggcgaccaca gctttattgg ttctagcacg aatatcatcg
     1381 gacctgtcga ggtagctaaa aacagttcga ttgctgccgg gtcaacaatt acagacaata
     1441 ttccagaata tgcgctggca attgcgagag caagacaagt caataaagaa ggatatgcaa
     1501 aaaaactccc ttatctaaac taagactttt gcaaaatcac ttgatttatt taaaaaatat
     1561 gactactatt tattaggtag aattctagag ttgtgaaaaa gaaagtggag gtccccatgt
     1621 caaaacatta ctttgaccca agattaaaaa tttttgcgtt aaattccaat cgcccattag
     1681 cagagaaaat tgcagcagca gtcggcgtcg agttaggaaa atcatctgtt actcaattta
     1741 gtgatggaga aattcaagta aacattgaag aaagtattcg tggatcgcat gtctatgtta
     1801 ttcaatcaac gagcagccca gtcaatgata acttgatgga gttgctgatc atgattgacg
     1861 cactgaaacg agcaagtgca aaaacaatca acgtagtcat gccttattat ggctatgcgc
     1921 gtcaagaccg taaagcacgt gcacgggaac caatcacagc gaaattagta gcaaacatga
     1981 ttcaaaaagc tggtgcaaca agaatgctga ctttggacct tcatgcagtc caaatccaag
     2041 gtttctttga tattccagtc gatcatttga tgggtgcgcc acttattgcc aattatttct
     2101 tagaaaaagg aatcaaaggc gatgatgttg tcgttgtttc accagaccat ggtggtgtca
     2161 cacgggcacg taaattagcg gaatttttga aatctccgat tgcgatcata gacaaacgcc
     2221 gtccaaaagc aaatgttgca gaagtaatga atatcattgg acaagtcaaa ggcaaaactt
     2281 gtgtactgat cgatgatatg atcgatacag caggtacgat cacattggct gccaatgctt
     2341 tgaaagaagc tggtgcaact agtgtgtatg cttcatgtac acatccagta ttatccggcc
     2401 ctgctttaca aagaatagaa gattcagcta tcgagcgtct agttgttaca gactcgatct
     2461 atcttccaga agatcgtaaa atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg
     2521 gagatgcaat caaacgtatc catgaaaaca aaccagttag tccattgttt gaaacaaaaa
     2581 ataaataata aataaactca caaaggggag tccaattggg ctgagattga atctatttct
     2641 aaacccttcg tacctgtatc ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg
     2701 actattttcg aaagctgctt cctcctttgt gactttttta ttcaaaggag gaagtttttt
     2761 tatgggaaaa aatagagttt gggtagaagg aacgattgtt gcagcgttag cgatggtgtt
     2821 gtcgttgatt ccgatccaga tcggcagcag tttttctatt tcattagggc agattccatt
     2881 gactcttttt gcgttaagaa gagggacaaa ggcagggatg ctggcaggtt tcatttgggg
     2941 catcctacat tttccgttgg gacaagttta ttatttaagt gtcgttcaag tgttgattga
     3001 gtacccgatt gcctatactt ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa
     3061 aggctatgta gacgataata cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat
     3121 tggcgcactt tccagatatt tttggcattt tattgcagga gtggttttct ggggagcgta
     3181 tgcactatgg gggatgaatt cttggctctt ttctttcgta atgaatggtg ccagcgggtt
     3241 gagtacagct attgtaacga tcatcgtttt attagggatc caacaaacga ctccttcgat
     3301 ttttgtgcca gataaacgac aggagctgta aataaaaaag aagcaagaaa cttgagacag
     3361 aatcatgtcc tagtttcttg tttccttttt attttttgat aagaaataga tctacttcaa
     3421 tggcagataa gaaagatcgg catcttctac caattgccag tgtcgatcgg tgacagagta
     3481 gattaaagta gcgatgattt cctcttctcg ataccgggtg ccttttaatt tccaaatata
     3541 atcttcaatc acatagattg ggatacgata tttaccatcg atcaaaccaa cttgatca
//
LOCUS       DACEOE010000202         3597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEOE010000202 DACEOE010000000
VERSION     DACEOE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.208"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..671
                     /locus_tag="IW774_002497"
     CDS             <1..671
                     /locus_tag="IW774_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1154821.1"
                     /translation="QMFFLNEGWEATFKYDGKEGLDAFLENPQHWDMITLDLNLPTLD
                     GMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVTKPFSPLTLIARMKALH
                     RRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPIEGLTPKEFDLLFTLAK
                     KPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVGPQVIQTVWGVGYKFDD
                     SGVA"
     gene            671..2119
                     /locus_tag="IW774_002498"
     CDS             671..2119
                     /locus_tag="IW774_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1154822.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2189..2698)
                     /locus_tag="IW774_002499"
     CDS             complement(2189..2698)
                     /locus_tag="IW774_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154823.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(2688..3413)
                     /locus_tag="IW774_002500"
     CDS             complement(2688..3413)
                     /locus_tag="IW774_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154824.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1283 a          609 c          739 g          966 t
ORIGIN      
        1 tgcagatgtt tttcttaaat gaaggttggg aagcaacttt caagtatgat ggaaaagaag
       61 ggctcgatgc ctttttagaa aacccacaac attgggatat gattacacta gacctgaatt
      121 tgcccacact ggacggtatg ctagtaggaa gagagatccg caaagtttca agtactgtgc
      181 cgattatcat gctgacagct agagattctg aaagcgatca ggtgatcggc ttagagatgg
      241 gggcagatga ttatgtcaca aaacctttca gtcctctgac gcttattgct cgaatgaaag
      301 ctttgcatcg ccgtagtgag ttggcagaag caagagaaga agcagtagtt tcagatgatc
      361 attttgatat cgagactagt catttcaaga tgaatacgaa aacacgagaa gcgtatctgg
      421 acaaccggcc aattgaagga ctgacgccta aagagttcga tctattgttt actttagcaa
      481 aaaaaccacg tcaagtattc tctcgggagc agttgcttga attagtctgg gattatcagt
      541 actttggtga tgaacggaca gtggacgccc atatcaagaa attacgacaa aaaatcgaaa
      601 aggtcggacc acaagtcatc caaactgttt ggggagtcgg ctacaaattc gatgattctg
      661 gtgttgccta atgcgttatt tgtatcagca actcttagct ttctgcggaa tggtcgcgtt
      721 gatcttattg atcgttggag tttcttttac acaactgaca aaacaaacga ttgaagaaaa
      781 caattaccag caactgtttg gatatgctga atcagttgaa aagacaacgc aaacctatgc
      841 cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa aatgcattgc tgctgacaga
      901 gcaagtactg acagaacaaa atgtgaactt tatatttatc gataaaaacg aacgtgtcat
      961 ttatccgact aatacagctt tactgaattt taccttaaca gctgatcaat ggaaaaatct
     1021 gaggaatgga cgtcaagaaa aattcacatc gaataaaaat attttaggac aagcacaggc
     1081 gacctcttac gcacttgtgc catttaattt gaatcatgaa ttctatggag cacttgtagt
     1141 tagtcaacct gcgcgaaata tcgataacag cgttcgtcca attacactga acctgttcaa
     1201 aggatttatt ttttccagta tcgttgcagt tattgccagc tatgcttttg cagcattcca
     1261 agttaagcgt atcaatcggt taaggaatgc gacgaaagaa gtgacgaatg gtaattttga
     1321 tgtacagctc cccgtacatg acaaagatga atttgatgag ttagcagatg atttcaataa
     1381 aatgacgaat tcattgaaag aatctcaagc agaaattgaa gaacaagaaa atcgtcgaag
     1441 acaatttatg gcagatgctt ctcacgagat gagaacacct cttacgacaa tcaacggact
     1501 tctggaaggg ttagaataca atgcaatccc tgaaaatcag cgagaaaacg cgattaaatt
     1561 gatgaaaaat gaaacagagc gtctgatccg tttggtgaat gaaaacttgg attatgaaaa
     1621 aatccgtacg aatcaaatca gtatggtcat taaaaaattc aatggaacag aaacattgaa
     1681 aaatattgtt gcacagttag aagctaaagc agaagcagca ggagatacat tgacattgaa
     1741 agccgttgat gatattgatg tttacgctga ttatgaccga tttgtccaaa tcatggtcaa
     1801 tattattcaa aacgcgatac agtttactga aaacggccaa atcacgatta ctttagaaaa
     1861 aggctatctt gaaacgatca tcacgattga agatacggga attggaatgt ctgaacaaca
     1921 aatgaaaagt atctgggatc gttattataa agtagatcct tcaagaaaaa atacaaaata
     1981 tggtgaatca ggattaggtt taccaatcgt gcagcaactt gtacgcttgc ataaaggaaa
     2041 attagaagta gaaagcgaac taggaaaagg aacaaaattc gctgttcgtt tgcctgacgt
     2101 agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat catgaatcga tcttctatac
     2161 cgcttgtttt tactataaaa tgattgattt agcccgcggg ctctaaacga tcaatataag
     2221 gactttgagc aatagtagca tcatgagtaa agagtaagat accttgattt tcttcatgag
     2281 ctaaataaga aagcaagtgg agtagctctt ggatctccaa tacactaagt tcatcaaatg
     2341 tatcttcaat aacaatattg tttcgcttgg aaagcaaggc atgaagtaat tgcagcttta
     2401 ctttttcaaa agacgtcaac tcagctgccg cacaattaag caaggaagga gagatatcaa
     2461 gtttttcttg ataggccaaa cgatcttgtt tgatttcttt ttccggtaca tccaacagga
     2521 catggtcttt aagattcaga taaggaaaca tcggccaatt tttttggatg atcgcaaaat
     2581 tatccagttt tgcaacaaga acctcttttt tcaagtgctc caatatttta tgctgttttt
     2641 cgagaggaca caaaataacg gtcaagtgtt cagcatgaat caaatattca ggtttcatta
     2701 cgtccaggcc tcctactttg caaatgatgg ataaaaaaag tgaaagggaa tagactaaga
     2761 atgagacagc atccactgag cccaataaga gtttgggcaa atcccttaag tatatctgtg
     2821 aaaaaaagat cattttggaa atcgatggta aaaaaagtct gttgatccat cggaattgta
     2881 agtccgctta ccgaagtcaa gatatcacgg atatctggca gttcagtagt ctttaagaca
     2941 agaaaattga tcctttgaag aagcgcttca taaactgact ggaagcttac gatccacaat
     3001 aaaaccaaga tacaacaaag aaagagagga aagaacagct gccagaaaat aaaaaggaat
     3061 aatttggttt tagaaagacc agtcaggtgc caattgatca attcctgctt gttttgcctg
     3121 actgaatagt aaaagaaaaa gataaaaagg aaggcaaata gaaaaaataa actgaatatg
     3181 ccttttatat aaaatcggct agctaattct aaagccggaa gaagctctgt agatgcagaa
     3241 gcgccaataa atgaaagccg atcttggact tgattggcta aagccgattg caaatggtaa
     3301 agagtcaggc atgctatcaa taaaaaagca gaaaagcttg aaaataaaca atagagtatt
     3361 gtctgctttt tgtaataaat taatcgagca gtagcatagc ggattatttt catacttttc
     3421 acctctataa aaagtataga aaatgaatat gaataaagta tgaaattcct ttagtgaaat
     3481 cacgaattaa aaccagaaaa tattttgtaa aaagcaagag gttgtgaaga agctgcccta
     3541 ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg agcagcg
//
LOCUS       DACEOE010000203         3587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEOE010000203 DACEOE010000000
VERSION     DACEOE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.209"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(46..270)
                     /locus_tag="IW774_002501"
     CDS             complement(46..270)
                     /locus_tag="IW774_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154825.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(290..658)
                     /locus_tag="IW774_002502"
     CDS             complement(290..658)
                     /locus_tag="IW774_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1154826.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(855..1565)
                     /locus_tag="IW774_002503"
     CDS             complement(855..1565)
                     /locus_tag="IW774_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154827.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1583..2080)
                     /locus_tag="IW774_002504"
     CDS             complement(1583..2080)
                     /locus_tag="IW774_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1154828.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2117..2569)
                     /locus_tag="IW774_002505"
     CDS             complement(2117..2569)
                     /locus_tag="IW774_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1154829.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2704..3309)
                     /locus_tag="IW774_002506"
     CDS             complement(2704..3309)
                     /locus_tag="IW774_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1154830.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT          943 a          726 c          575 g         1343 t
ORIGIN      
        1 tttctttatt gtgttaataa agtttcttca ttctagtctc aaaaatcaat agtgtgattt
       61 gattttacca tttcgtttct gttcttcttt gatgaattct tttgtatcga caggattcag
      121 attatttagt ccgccaaaac ctgtatcgac catttttccc cagccttttg tcttcttttt
      181 tcctgatttg agattctttg ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa
      241 ataaccttct aatcttccca ttagatccac tgtttttcct cctttttatt caatcagacg
      301 actcagcttt tcctataaca aaataattgc cttcagggtc agaaaaattg aaagtcaaga
      361 tgccattgtt atctgtgatt tctcctggtt cttccatgtt ctcgtagata gtttcaaatt
      421 catccgtaaa aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa aacttctgca
      481 caaatacttt tgggaatacg cctaattcta attcttttga gatgctgagt acaatactct
      541 taaattcttc tggcaattcg attgtttcag aaatttctgc accaattttt tctacaaaga
      601 atcggcaaat catctctacg tcattgacat aaagcatcac acgtgttttt tgtatcatca
      661 tttgaccctt ctttctattt gattttcttt cctgtctata aactgattcc attatatcac
      721 gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa ataaagcaat agtttctagg
      781 attgctttcc tccacaaact ttttttacaa aaaaaaaaac ggcaatgaat gccgctcttc
      841 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
      901 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
      961 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
     1021 gtcagcatcg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
     1081 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
     1141 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
     1201 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
     1261 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
     1321 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
     1381 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
     1441 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
     1501 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
     1561 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
     1621 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
     1681 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
     1741 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
     1801 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
     1861 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
     1921 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
     1981 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
     2041 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
     2101 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
     2161 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
     2221 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
     2281 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
     2341 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
     2401 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
     2461 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
     2521 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
     2581 tttattttgt ttgtaagact agttcacctc tttttgttgc aaatgcaact attttcaata
     2641 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
     2701 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
     2761 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
     2821 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
     2881 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
     2941 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
     3001 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
     3061 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
     3121 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
     3181 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
     3241 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
     3301 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
     3361 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
     3421 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
     3481 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
     3541 gctgttctta ttaattgagg caggacagct ttttcacaac ctcccca
//
LOCUS       DACEOE010000204         3572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEOE010000204 DACEOE010000000
VERSION     DACEOE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.210"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            44..1033
                     /gene="galE"
                     /locus_tag="IW774_002507"
     CDS             44..1033
                     /gene="galE"
                     /locus_tag="IW774_002507"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1154831.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1045..2529
                     /gene="galT"
                     /locus_tag="IW774_002508"
     CDS             1045..2529
                     /gene="galT"
                     /locus_tag="IW774_002508"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1154832.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2553..3533
                     /locus_tag="IW774_002509"
     CDS             2553..3533
                     /locus_tag="IW774_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1154833.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1213 a          657 c          790 g          912 t
ORIGIN      
        1 agaatcaaca aacacaattt tactaatcaa aggagtgttt tttatgacaa ttttagtact
       61 aggcggagcc ggttatatcg gttcgcacgc tgtagaccag ctagttcaaa aggggtatca
      121 agtagctgtg gtagataatt tgctcacagg tcacaaacaa gcagtacatc cagatgctca
      181 tttttatgaa ggagacatcc gggataaaga atttctacgt tccgtatttg aaaaggaacc
      241 gattgaagga gtaatccatt ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc
      301 attgatgtat ttcaacaaca acgtatacgg tatgcaaatc ttacttgaag tcatgcacga
      361 gttcaacgtg aataaaatcg tattctcttc tactgcagca acttatggag agccgaaaga
      421 gtctccaatc acagaagata caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt
      481 gatgatggaa aaaatgatga agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt
      541 gcgctacttc aatgtagccg gagcaaaagc agatgcatct atcggagaag atcatacacc
      601 agaaacgcat cttgtcccaa tcattttaca agtagctctt ggtcaaagaa aagccttggc
      661 tgtttatgga gacgattatg atacaccaga tggaacatgt atcagagatt atgttcaagt
      721 agaagatttg attgctgcac atattttagc tttggaatac ttgaaagaag gaaatgaaag
      781 caacttcttc aatttaggca gcagcaaagg atactcagtc aaggaaatgc tagaagcagc
      841 tagagaagtg acaggaaaag aaattccagc agaaatagca ccaagaagag ctggagatcc
      901 tagccgatta gtcgcttcaa gtgaaaaagc gagagagatt ctgggctgga aacctgaata
      961 cacagatatc aaagcgatta tcaaaacagc atgggattgg catgtcagcc atccaaatgg
     1021 ttatgaagaa taggggaatg agatatgtca atcagtcaaa cgatttgtga ttttgccaca
     1081 cttgcgatcg aagctggcgg atggatggaa atggaccgct tatatttaca gaatcgtctt
     1141 ttagggatgg taggagaaga tgctctagaa agcattgaag taagaccagt taaggaagaa
     1201 tctgtagact tattggatca gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa
     1261 ttgccagcag aacaagagat ttttgaagcg caattaatgg attttctcac accgccacct
     1321 tcagtcgtca atgcattctt tgcacagcac tattcgaaaa gcccagagga agcaactgag
     1381 tatttcttta atttaagtaa gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac
     1441 atcaaatttc cggcagagtc ggagtatggt gaactggaaa tcacaatcaa tttatctaag
     1501 ccagaaaaag atccaaaaca aatcgcagca gaacgcgagg cggtaaaagt agactatcct
     1561 acctgcatgc tttgcatgga aaacgaagga tatagaggtc gcttgaacta tccagcccgc
     1621 accaatcacc ggattattcg aatgaatcta gacggtgaaa gctggggatt ccaatattcc
     1681 ccttatgctt attacaatga acattcgatc attttatcag aacaacaccg cccgatgaaa
     1741 atcacaaagg aaacatttgc tagactgctt cggattactg aagtcttgcc tcattacttt
     1801 gttggttcga atgctgactt accaattgtt ggaggatcga ttctttctca tgatcattat
     1861 caggcaggac gtcatgaatt tcctatggca aaagcaccga tagaaaaact tatcaaacta
     1921 gaagattatc cgcaagtcat cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg
     1981 caatcaacag agaaaaactt gcttattgaa gcagctaact cagtattgga aaaatggaaa
     2041 gcatattcgg atgaaacagt atctatcaga gctttttcag cagatggtac accacaccat
     2101 acgattacgc caattgcacg aagaagggac aattactttg agttggacct tgtccttcga
     2161 gacaacaatg tttcagaaga acatcctgat gggatttttc atccacataa agatgtccaa
     2221 catatcaaaa aggagaacat tgggttgatc gaagtaatgg ggcttgctgt tttaccacct
     2281 cgtttgaaac cagaactagt agaagtagag catttcttat taggagaaga aaaccagatg
     2341 gctgaatatc atcgtccgtg ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa
     2401 gatacggtca ccgatgtcgt acaaaaatct gtaggtcagg tttttgcacg tgtactggaa
     2461 gacgctggag tattcaagcg agatgaaaaa ggacaggcag cgtttgaacg atttactgcg
     2521 attttataaa cgtaaaaaag gaggagagac aaatggcaac aattaaagac attgcgcagt
     2581 tggcaggagt ttctcctgct actgtttcaa gagttctcaa ctatgatcct gatctttccg
     2641 ttggcttagc cacaaagcaa aaaatttttg aagcagcaga acgcttaaat tatacgaaac
     2701 atcgtaaaaa tgcagcaaat actaagcaaa cgcttcgcct gatccagtgg tacgacgata
     2761 cggaagagct tgaagatctt tattatttat ctattcgcct aggaatcgaa aaaaaggcag
     2821 aagaagcaaa cgttacttta ttgaaagaaa cgtgggattc cattagtgat cttccggcag
     2881 atggaacgat cgcactagga aaattcgacg aagctcagtt attagaaatg aaaggatccc
     2941 aaaatgcctt gcttttggta gattctgacg gcacttctca aggaatcgac tctctagttg
     3001 tcgactttaa gtcaagtgtt caaaaagtca ttgaacactt tgtctcccat aaagcgcaac
     3061 atatcgctat gcttgctgga acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc
     3121 ccagaattat ggcatttcaa caagttttgg cagaaaaagg ctacgacgaa ggtccaatta
     3181 tcgaagcaga tttttctgtc gagtcaggat atcaagcagt gaagcagtat ttaaaaacga
     3241 atccgcatgt tcctgatgcg ctttttgcag ctaatgatgc attagcgatt ggcgcactga
     3301 aagcgattca agaaaaaggt ttagtggtac cagaagatat ttctgtgatg ggcttcaatg
     3361 atgtcagtgt agcgaaatat gtaacgccct cgctttctac tgtaaaagtt tataccgaat
     3421 ggatgggaga gctagcagtg gagacgattc ttcaattaag taattccaaa gcaccggttt
     3481 caagaaagat tattgtggaa acagagttga ttattcgaga atcaacgaaa taaaagatgg
     3541 aaaagctgtg ggaaaatgga gtgataccgc ag
//
LOCUS       DACEOE010000205         3540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEOE010000205 DACEOE010000000
VERSION     DACEOE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.211"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            703..1377
                     /locus_tag="IW774_002510"
     CDS             703..1377
                     /locus_tag="IW774_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1154834.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1374..1715
                     /locus_tag="IW774_002511"
     CDS             1374..1715
                     /locus_tag="IW774_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154835.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            1745..2104
                     /locus_tag="IW774_002512"
     CDS             1745..2104
                     /locus_tag="IW774_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1154836.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2307..2486
                     /locus_tag="IW774_002513"
                     /pseudo
     CDS             2307..2486
                     /locus_tag="IW774_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            2628..>3540
                     /locus_tag="IW774_002514"
     CDS             2628..>3540
                     /locus_tag="IW774_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1154837.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAE"
BASE COUNT         1217 a          567 c          759 g          997 t
ORIGIN      
        1 ggagagcacc gtttattcta aaaaagaact gcgaactctc gcagttcttt ttttaaaata
       61 cgggataaag gaagtttcca gcagacaaga gaagaaagca aaagaaattg ctatttgatt
      121 cacaaggttc tataacgaaa tcgcaatcaa tcaaacattt acaaagaagg taattcaaat
      181 ggaaatctga tttgatgatg cttctgctgt tgaaataaag cagcatctag cacctgaaaa
      241 caaactaata gtaacattcg aagatggagt aggtccgtat tttcagcatg caataatcca
      301 tatgtaagtt caattcaccc ttgaaatcat cggaccagat aaagttatga ccaatctatc
      361 ccaagcaatc tagaagagat attagtaaag ggacatttac tagaagtctt agatgagcct
      421 ataaatatat gttctgatgg cgtcatcagt ggtctgattg acggaagctt aggtgtgatc
      481 gatctctctg gaccaaacaa cgaaggaatc aaggtgaatc cggcacgtta agaaaaaaat
      541 agcataaaaa actagacact tgatgagcgt tcattcaatt gtttgaaggg actaggctgg
      601 tgtcattttt tatacatata ttttctcttt cgtcttgata gggcttatgg gataatataa
      661 ggaagaaaat catcgtttta ttcaaaaagg agtttttgac aaatgaaaaa acgtatatta
      721 tttatcggtg atagcattac agacgcagga agagaccgag cagaagcaac tgatctaggt
      781 aaaggttatc cacttttgat tgccggaaaa ctaatctccc aatatccaac gatggaattt
      841 gaatgcatga accgtgggat aggaggagac aaaattgcag atctgcaagt acgctggcaa
      901 gaagattgtt tagctttaca acctgactat gtatcgattt tgatcggaat caatgatacc
      961 tggcataata ctagcgatca ggctatattc ggaactgaga aatctgctca gcaatttgaa
     1021 tccgtttatc gaaaattact aaatgaactt caaacagtgg gaatctgcaa catcatcttg
     1081 atggagccgt ttgtacttcc tataaccgac gaccgtcatg gctggcgtaa agacttagac
     1141 cctaaaatcc aaattgttcg taaacttgct aaggaatatg gcgcattgct ggttccttta
     1201 gatggtatat tcaatgcttt agggattgtt tatggttttt cgaaatatac aagagaagat
     1261 ggggtccacc caacgattgg cggacatgag atcattgcac aaagctggat agatgcaatt
     1321 gccgcagtcg atttttttag tggagataaa aatgagcgaa agaaaagtga gaaatgatgg
     1381 aaatacggtg ggcagtaaaa gaagatctac ctcaaatcaa tcagctgatt actgatcatc
     1441 cagaatcatt cgtaaaagca aaaacggcag ctgacctaaa tgataagaat aaactattgc
     1501 tcgttgctgt aacaaaagaa aaacggatta tcggatttgc gcaagcggac gtgcaacagt
     1561 cagaagaacg attggatcag cgaccatcag gaaaactaat ccaacacaca ttagtaacaa
     1621 aagaaaatac ggaagaaatc caaaaaaaat ttgcagaata tttgatactg gaagctaaaa
     1681 aaagaagaat aaaatcattt tttgcccagt tttaatcaaa aacagtaata gaaggagaaa
     1741 tacaatgagt tgtttaggaa atgtcatctg gtttatattc ggcggactaa tcggtggtct
     1801 tagctggtta gcagcgggga tactttggtg tatcacgatc ataggtatcc caatcggtat
     1861 gcaatgtttt aagatcgcct ccctttcttt tgcgcctttt ggcaaagaag taatttatca
     1921 ccagtctggc atgtcattga ttgttaatat catctggctg atcgtttcag gcctaccaat
     1981 tgctctcgca catgtcgtca gcggcatcct tctatgtatc acgatcatag ggataccttt
     2041 tgccaagcaa tctttcaaac ttgctcgatt ggcgttgatg ccattcggtg cagaaattgt
     2101 ataaaagaga ataataaagg ttagataaag catagagtca ggtgggatac atgtatttca
     2161 cctgactcta tgcgttctcc tgtataagtt cttccatgtg acttttacgt cttttattta
     2221 tatggggtta ctgtttatgg aaagttgaaa taattttcat taaagaactc tttctatatt
     2281 tttgaaagcg tagtggagct tcgaatatct gggaaggaca ttttgcaggg attgaaaaaa
     2341 gatattgtcc tatcgaaaaa ttaatgaatt tagacaaatt gccagcaaca ggcttcaaac
     2401 ttagttgcta tcctgtcaag atcaagaagg caagtgcggg ttggataaga gcagtggcaa
     2461 tgatcgaaaa gaaaaagaaa gaataagcca aaacatctgt atggaagaaa ttttccgatg
     2521 cagatgtttt tcattttttc tttcggatac cttgacatat gaataaccgt tcatttataa
     2581 ttagttatga agagatattc atatgttgag gcgaggaggt cattaaaatg aaaagttaca
     2641 gattagaagg tttagattgt gcgaactgtg caatgaaaat cgaaaaagga gttcagaaga
     2701 tcaatggagt caaggaagca acggtaaact tcacttctgg aaaactaacg attgacgcag
     2761 aggaagatca tttggctacg attgaacaag aaataaaaaa agtagtgaag gaattagaac
     2821 cagatgtcaa agtaacagaa atagataagg aaaaagtatc tgaacatgga aatgaaaaag
     2881 agagaaatac gctgttccgg attttatttt cccttgcagg tatcgcttta ttgctattat
     2941 ttgattttaa tgaaccaatt cgcttgatag ggtatttatt gatttatctg ctgataggat
     3001 atgatgtagt gaagaaggca gtgatgaata tcgtcaaagg aaaaatcttt gatgagaatt
     3061 ttcttatgtc tgttgctacg attggcgcta tgttgatcgg tgaatatcca gaagccgtag
     3121 cagtgatgct attttatcaa atcggcgagt atttccaagg attggcagtc agtcattcaa
     3181 gaaaatcgat tcgcgaattg atggcgatcc gtccagaaac agctcacgtg cagacgacgg
     3241 aaggattgat gaccgtgaac cctgaagacg tgcagatcgg tcagtttgta cttgtgaagc
     3301 ctggtgaacg tgtaccgtta gatggaacga tcatcgaagg agaatcatta gtcgatacgt
     3361 cagcacttac aggagaatca gtccctaaat ctgtatatgt tggtgaaaca gtattaagtg
     3421 gatttatcaa taaaaataaa ccatttttag ttcaagtaga aaaatcttat gaaaattcta
     3481 ctatcagcaa attattagaa ttagtagaaa atgccagcag taaaaaagca ccagctgaaa
//
LOCUS       DACEOE010000206         3497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEOE010000206 DACEOE010000000
VERSION     DACEOE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.212"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            180..539
                     /locus_tag="IW774_002515"
     CDS             180..539
                     /locus_tag="IW774_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154838.1"
                     /translation="MEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDE
                     RTLLLIEDCYYLWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFRE
                     KEFSNQKKKIDKKLKKR"
     gene            complement(710..1051)
                     /locus_tag="IW774_002516"
     CDS             complement(710..1051)
                     /locus_tag="IW774_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1154839.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1137..1844
                     /locus_tag="IW774_002517"
     CDS             1137..1844
                     /locus_tag="IW774_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1154840.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            1831..2154
                     /locus_tag="IW774_002518"
     CDS             1831..2154
                     /locus_tag="IW774_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1154841.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2243..3277)
                     /locus_tag="IW774_002519"
     CDS             complement(2243..3277)
                     /locus_tag="IW774_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1154842.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTRAGS
                     NQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKILV
                     INGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELGI
                     DQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMFA
                     KTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHMLG
                     KVVVSGPFNG"
BASE COUNT         1008 a          731 c          623 g         1135 t
ORIGIN      
        1 cctctcttgt acgggagctt ttaacagaga ttcttataga acatccgcac tttatcgaac
       61 gcttcaaagt taagatgcca tttcatgcaa taaattattc agataaatta acaactatca
      121 tccataaata cgatcatatt atcaagaaaa acaaaaacag gaaaacagcg aagttcatca
      181 tggagatgcg aaagttcatc caagaagcag ttgaatcgct tattcagcaa aatgcctatc
      241 ttcccgcatt cgaattgatc aatgaagtga ttgctactct cgaaacattc tattgggagc
      301 ctgaggatga acgaaccctt cttctgatag aggactgtta ttatttatgg aaagaattat
      361 tagcagaagc acctcatgca gaaaaaaggc aaatgttctc ttggttcgtc tgccaagtcg
      421 atcatactga cgcctcttat tcaaaacgct actctataaa aatcctgaaa gaagactttc
      481 gggaaaaaga attttctaac caaaagaaaa aaatagataa aaaactaaag aaacggtaaa
      541 gaaagacgat ttcaatgaaa aatagcaagt aaacgataac aaccatccta aatccacaag
      601 aaatacagca gaccttgctt taaaaacacg aatcatgcaa aggatgaagt aaagaagact
      661 taacgcgtac ccaatattag tcatttgact attaggtacg cgttttgttt tataagtcat
      721 gacaaatttc cttcgttgct ggatagattt tccggcattg ttgatagatt ttatgatact
      781 tttctacatt ttgtaagaga ggttccactg catctgtata ttggataaaa ttctgacaac
      841 aactttcagg tgtgtcgaac caccctaacc ctagtgcagc aatcattgtt gcgcctaatg
      901 caggtccttc ctccacactt aaacaataga ccgttgcatc gaaaatatca gcttggattt
      961 gtagccatgc aagatttttc gcgcctcctc ccaccgaaac aatacgtgta aatctgcgac
     1021 ccgctagttc tttacgtttc aatcccacca ttgacaaatg ctcattaaaa atttagaata
     1081 ttcacaagtt taacgacaat tatgaaaatt tatgtcgtgc taaaggagtt ttgttattgg
     1141 atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt ttcggttata
     1201 tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cggatttcat ccactagtcg
     1261 tcacgttgat gtccctgctg gtctatgctg gttctgccca atttatcaca gtcagcatgc
     1321 ttgctagtca cagcccattg ctttccatcg ttttctcgac ctttctagtc aattcccgaa
     1381 tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg cttcaaaatc
     1441 tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat aaattgaact
     1501 atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt tcttatttga
     1561 cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact gatccgaaaa
     1621 agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat cttcaagtca
     1681 tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc actttgattc
     1741 tggtatatgt cggactgatt tttatcccaa gcaacttagt gattgtagtt gtaacactga
     1801 ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac attgctgact
     1861 attttaggtt gtactttagc aacttggctt tctagggtcc taccatttgt cttactgaaa
     1921 aaattcgatt taccgcaacc gttgcttgag tatttaagtt ttgtcccgat cgtcattatg
     1981 tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc gcagatcaat
     2041 ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc aaaaagctta
     2101 ttggtgatcg tcttggcagg aattctttcc ctttcgttga ttcgcttatt ataaataaaa
     2161 aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa gtcaaataag
     2221 aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg acttttccta
     2281 acatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca ttcatcccat
     2341 ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa gcagcaatgt
     2401 gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta gcgaacatat
     2461 actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac ggctctttga
     2521 tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgcttgatt tgatcaaagt
     2581 agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct aattcacgaa
     2641 aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt tttttcagcc
     2701 attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa tgcgctagtt
     2761 gattcaaaat cgaaccgacg cccccggatc cgttgatcac taagattttt ttcccgcgat
     2821 tggcattttc ttctggagtt agaccaaatt tttcaaataa caattcataa gcggtcaatg
     2881 aggtcaacgg caaggctgca gcttcttcat ctgaaagatt ttttggtgca agagcgacaa
     2941 tacgttcgtc aactagttgg tattcttgat tgcttcctgc tcttgttgta cctgcgtaaa
     3001 aaacgcgatc gccgacagaa aatttttgca cttcgtcacc tacagcaaca actttcccga
     3061 ctccatcaaa acctaagata cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg
     3121 tatcgacagg attaacagat actgcgttga cttttaccag aagatcccgt gaactgggta
     3181 caggtatctg ttctttttca tcaataaagc tttttggatc atcgatcggc aacccttcat
     3241 aaaaaccgac taccttcatt aattcctttt ctgacatacg aattcctcct tagcttcatc
     3301 aagttggagc atccatacag tgttcatttc aatatatata gtatgctatt tgttccacct
     3361 tttttattca tcataattat tctcttactt aaagtaaact atttgcactt ttttggttaa
     3421 aaaagaggtt gcgacaagac ttttgtcaca acctcttata aacggtgttc tcctaccaac
     3481 tcttcggtca caagtca
//
LOCUS       DACEOE010000207         3488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEOE010000207 DACEOE010000000
VERSION     DACEOE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.213"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            683..1909
                     /locus_tag="IW774_002520"
     CDS             683..1909
                     /locus_tag="IW774_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1154843.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(2017..2487)
                     /locus_tag="IW774_002521"
     CDS             complement(2017..2487)
                     /locus_tag="IW774_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1154844.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            2809..3387
                     /locus_tag="IW774_002522"
     CDS             2809..3387
                     /locus_tag="IW774_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1154845.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         1268 a          536 c          659 g         1025 t
ORIGIN      
        1 ttttggctaa gtaaatgcat ttgttcactt gacatgtcct aactaattat caagcaagga
       61 acattttctt cgttagtaat ggattttaaa gaaatttcag tttttggatg ttgaataaaa
      121 gaccatttgt gaaaccacta gtatacatac tcattaccat gtcaattcta ttactttaaa
      181 agaaaatcgt tttactttga agaggagcat atctatccat taaaagatga tggtaatgca
      241 atcactagtt cgagtcattt taagagccat gcagtgagct ggaaataaaa cgaatagaag
      301 atgggtttaa caaaaggacc agtcatagga agaaattgtc gtgaaatcga atgataatct
      361 gaacggtagg caaagactgt taaaaagatg ggaaaggtat gtgcgatcat tttactgcac
      421 gattatttgg aaaatacctt gttgttcgct ttttctggaa aaaatacata aaaaaagata
      481 aaacgattgc gaactgcgtg tattcggtct ttcgatcttt ttcacggtat ggtatgatag
      541 ttaagaattt tgagaaattt taaaaaataa taatcgttac acaaaaatgt tttttaaagt
      601 tttcttatga ttaaaaagtg aatttgctga ttgaacagtt atatgagaca atattttaga
      661 aaatgtgaaa cagaggtgta ttatgaagcg atggcaaaaa gttctattaa ccttgttagg
      721 ggtattcgtt ttgctgattg ccggattatc agcatacgga atcagattta tgggagaagc
      781 gagccaaaca gtaaacaaaa tttctaaaaa ctctaatcga atatcctcca aacgttctga
      841 gagagtaagc attgatgaca aagaaccttt ttcggttttg ctattaggtt tagataccgg
      901 tggattagga agaacggaac aaggacgttc tgatacgatg atggtagtaa cagttaatcc
      961 gaaacaaaaa aaatcaacga tcatcagttt ggaccgagat atttatacga atatcgtagg
     1021 ttatggaacg gtagataaat taaatcatgc ctacgcattt ggtggtgtcg aaatggccat
     1081 ggattcaatt gagcaactgc tcgatattcc tattgatcat tatgtaacga tcaatttgga
     1141 tggcatggaa gatttgatca atgcggtcgg tggcatcaaa gtgaataata aaattgactt
     1201 tactttggat ggtgtccatg taccagtagg tgaacaaaca ttgaatggag aaaaaggttt
     1261 agcctattct cgaatgcgac acgaagatcc agaaggcgat atcggtcgac aacgtcgtca
     1321 gcgggaagta gtgacgaaaa tcgtcaataa agttttaagt ttagatggcg taagcaatta
     1381 tagaaaaatt ttaaaagcag ttgagaacaa tgtgacgacg gacttagatt gggatgacat
     1441 gcttgatgta gcgacgaact acaccccggc gtttgaaaca atcaaacaag accagttgca
     1501 aggcgaagga caaatgatca atgatatcta ctatcaaatt ttaggaaaaa atgatcttct
     1561 ttcgatccaa aatgaactga agaaacaatt aaatataaaa acaagcgata cattacctaa
     1621 tttgaaaaat gagaatgctt caatgatgtt ctttgatgat tcggaagatg gcgattcgac
     1681 aaacggtgat ggatcgaata gtgaagggta caccgattat tcttcctatt cagaaaacac
     1741 atatgatcag taccaagatc cgaataccta ttatcagcaa acagaagata caagtcagta
     1801 ctatacacca agtcaagata ctcaaagtta tgatccttac gctggacagg aacaaaatgg
     1861 ttatggttat gaccagaata atgagtatgg aacaacaagt gggtactaga aaaaaatagt
     1921 ttggttaata aagagtgcca cctaaattag acaaaaaatc taacttaggt ggcacttttt
     1981 ctatgtgtta aattacagga cgtctctttt tagcgcttag tttaaaaaat aaattgaata
     2041 attcaataga gttgcgaata gtacccataa ataataagga atcatgagcc acattgccag
     2101 gcgatgatgc agccacaact gatacataag cactgacaag aaaataacta aaagtgtaat
     2161 atcgattgct gcaatgaaaa tatttcttaa attaaaaaat agagcagacc atataaaatt
     2221 aaaaatgaac tgaatcacaa aaagaccgac aagattgttt cgctgttttt tgttgttgtg
     2281 attaagtaac aaataaagag aaatacccat caatatatac agtaaggtcc acatcggccc
     2341 aaatatccag ctaggtggtg caaatggtgg aagttgcaaa gaatagtaat attttccggg
     2401 atttccgcta agcaaccctg ataaaaagcc taatatgaca attcctataa cacaaaccca
     2461 aaaacgaata tcttttatca tcttcatttt actaactcct ttctataata ttagtttacc
     2521 atgaaggctc acagcaagac ctgtatttag tttgtgaagt aagaaaaaaa ttgataattg
     2581 ttgttattaa aaagttgaac atttgtttat atgaatgatg tttttgaaaa agatcatatg
     2641 atttgattct catgttttct atttgtactt ttctcgtcta cttctgttat aatctgcatc
     2701 ttactttttt cacctttttt acttcaacat aaaattataa aagcataatg tttgtgttaa
     2761 tacaataaag cgtttacaat gtggttatca aatgaaggag gtttctacat gaaaaagatt
     2821 agtacgttat tgtttattgg gttagttagt ttagggttgt tcggttttag cattccaggt
     2881 tttgcgcatg gttatattac ttctccagga agccgtgcat acctaggaac atcagcagca
     2941 ggaaacttaa atcaaaatgt cggaagagca caatgggaac cgcaaagtat cgaagctacc
     3001 aaaaatacat tcattgatgg aaaaattgct agtgcaggag tcagcggctt tgagccttta
     3061 gatgaacaga catctaacag atggcataaa aacatggtta atccaggaac attaaatatt
     3121 acttggaatt taacagcaca acatcggaca agtacttggg attactacat taccaaacca
     3181 acttggaatc ctaatcaacc tttgaaattt agtgattttg aattgatcac aaaaatagac
     3241 gacaaagcaa ctgttccacc aaaaacagtc aaccaaacga tcacacttcc gcaggatcga
     3301 aaaggatata atgtgatttt agcagtatgg aatatcagcg atacgacaaa tgcattttat
     3361 caagtgatag acgtgaatat ccaataataa tcaaaaaggg ctgtgacaaa aactatgtca
     3421 caaccctttt tttaaataaa tagtgtcctc ctgttaactc ttcggtcaca agtacagtat
     3481 tcataaaa
//
LOCUS       DACEOE010000208         3475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEOE010000208 DACEOE010000000
VERSION     DACEOE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.214"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(271..756)
                     /locus_tag="IW774_002523"
     CDS             complement(271..756)
                     /locus_tag="IW774_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1154846.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1236..1877
                     /locus_tag="IW774_002524"
     CDS             1236..1877
                     /locus_tag="IW774_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1154847.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1896..2531
                     /locus_tag="IW774_002525"
     CDS             1896..2531
                     /locus_tag="IW774_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1154848.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2549..3364
                     /locus_tag="IW774_002526"
     CDS             2549..3364
                     /locus_tag="IW774_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1154849.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1138 a          567 c          737 g         1033 t
ORIGIN      
        1 tagaatataa tattttttgt taaatatgac tgaaatcaaa agaataaaat tccgatttat
       61 aatcgaaaaa agaagcattt ttatatatgc ttcttttttc gtagaatcaa taaatatgga
      121 tttggctaac tatggaagaa tactcgtatt agttttcagc gttcatacta gttgttacat
      181 tgtttgattg gccaaaagaa tttctgatat attttgtgct aaataaagaa aaagacgctt
      241 gaattgggaa ataagaaaac tactctatca ttacaaattt tcataaactg gcagatgttc
      301 taagtaaaca agtatctttt caaagtcgga aaaatccata atcaacgtag tggtattgat
      361 atttggatga aagcctacgg tatcttgacg atcaatagca gcatcgatca cgacttggat
      421 ttttttctct ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg gaacgcctag
      481 gaggtcagga agctgttctt cagaaacaaa agataagcgc ttttctttta attgttcagc
      541 tagttgtttt aaatcggcgt gcttttcatc aggcaagatg acaagataga tttgttttcc
      601 ttttcttgat ttcaataata gattttttac ttgcggacca ggtagggaaa aagggacatt
      661 tttgactgac gtaatcgcag gatgatcgac tttttgaaaa gagataccca gtttttttag
      721 tagttcataa gcttcttgtt cggttgcacg actcatcgcg catcaccctc ctttttttat
      781 tagtgtatca taaaaaagag ttgacaaatg aaaaataatc atgtaaatta cacgtaaagt
      841 taaaacaaat gacatcgaaa agaagagtag ccgttaggtg tttttagaga gtctctggtt
      901 ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt ctgaagcgta
      961 tagtaggaaa atccggggag cccgttacag ctgcggtcgt tgctagatga ccagataagg
     1021 ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa cgtcctttga
     1081 tcttcggatc aaaggacttt ttgttttcat ctgaacggca acgactgtgt gaggtttttt
     1141 ctgcaaggaa aaaataaaca gaggtggaac cacgtataat acgtcctctt ggcatttgcc
     1201 aagaggattt ttttatttta aaggaggaaa agaagatgga gtatttgatt gaagttatgc
     1261 cgcaattatt agtaggagca ctcacaacat tgaaattatt tttatttacc ttgattggct
     1321 cattaccatt agggattgtt ttagcattag gattgtcttt gcattttaag ccgctacgct
     1381 acttgcttca aacatatgta tgggttatgc ggggaacacc gctgttgttg caactgattt
     1441 ttatttttta tgggttgccg acgattggga tcgtttttga tcgtttcgaa gcagcactca
     1501 ttgcctttat cttaaactat gcagcttatt ttgcagagat ctttcgtgga gggatacaat
     1561 cgattcctca aggtcaatat gaagcagcgc aagttttgca attaagtccc tttcaaacgg
     1621 tgacgaaaat cgtcatccca caagtgacaa agattgtttt accttctgta gggaatgaag
     1681 ttatcaactt ggtcaaagat tcttccttga tctatatatt aggtttaggt gacgtaatgc
     1741 gtgctgggaa aatcgcgatg gaacgcgacg cgactttact gcctttgcta ggcgtggcaa
     1801 tcatctatct tacattgact ggtattctaa caatcgtaat gaaacaagtt gaaggaaaat
     1861 taaattatta ccgctaactg ataggaggaa gaaatatgat tgaattgaag aaagtgaaca
     1921 aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct aatcattcca gatgggaaaa
     1981 cattagctat cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt atcttggctg
     2041 gcttagaaag ttctgacagc ggagaatttt atttagacgg tgtaccgttc aacccattcg
     2101 agcttaaaat gaaagaacaa gttgtaggag tcgtattcca agacttccag ctatttccac
     2161 atctgaatat ttttgaaaac attaccttag caccaaaaat ggtattgaaa cagacaaaag
     2221 aagaatacac caaaaaagca gaagaattag cggcttcatt agggattagt gatttattga
     2281 atatctatcc ttatcagctt tctggcggac aaaaacagcg ggtagcgatt gccagagcat
     2341 tggctatgaa tccacgggta ttggcttatg atgaaccaac aagtgcttta gatcctgaat
     2401 tacgccagca agtcgagcgt ttgatcctat cattgaaaga agatggggta acccaaatta
     2461 ttgtcaccca tgatttagta tttgcagaaa atgtcggtga tcaattacta aaagtttccc
     2521 caattcatta agaaaggaga agatagagat gaaaaaaacc agttttctat tcgctttgat
     2581 cgcaggtctt cttctttttg caggctgttc gaacaaaaaa acttcagcag ataattgggc
     2641 gactatcaac gaagagaaaa aaatcgttat aggtttagac gatacatttg ttcctatggg
     2701 atttagagat aaagacggga agctcacagg atttgatatt gatttagcca aagcagtctt
     2761 tgaacaatat gggattacgg tagactttca gcccattgac tggtcaatga aggaatttga
     2821 actgaacaat ggaacgatcg atttgatatg gaacggctat tccaaaacgc cggcaagaga
     2881 aaagaaagtg caatttacaa aaccttatat ggaaaacgat caagtgctga tcacacctaa
     2941 agaatcagga atcacaaact ttggacaaat gaataataag agattaggtg cgcaaaatgg
     3001 gtcatctggt tatgatgtat ttacgaaaca accagaagta ttaaaagaga tagtcaaaaa
     3061 ccaagatgct gttttgtatg atagttttaa tgaagcattg atcgatctga aaagcagacg
     3121 ggtcgatggt ctgctgatgg ataaagtata cgcagactat tatttgaaac aaagaaacga
     3181 actagaagac ttcaacataa caaaaggttc ttatgagagt gaggattttg cagttggtgc
     3241 acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa gccctagatc aactagagaa
     3301 aacaggtgaa ttccaaaaaa tatccgataa atggtttggc gaagatgtta cgccgagaaa
     3361 ttaacaaata ggggactgtg acaaaagacg tgtcacagtc cctttttccg aataaacagt
     3421 gttctcctgc caactccttg tgactcggag ctgaatggca ggctcacaac ctctt
//
LOCUS       DACEOE010000209         3444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEOE010000209 DACEOE010000000
VERSION     DACEOE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.215"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..403
                     /locus_tag="IW774_002527"
     CDS             <1..403
                     /locus_tag="IW774_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ1154850.1"
                     /translation="IRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIE
                     AASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYG
                     DGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            376..1053
                     /locus_tag="IW774_002528"
     CDS             376..1053
                     /locus_tag="IW774_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1154851.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1043..2185
                     /locus_tag="IW774_002529"
     CDS             1043..2185
                     /locus_tag="IW774_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ1154852.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2198..3175
                     /locus_tag="IW774_002530"
     CDS             2198..3175
                     /locus_tag="IW774_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1154853.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3175..>3444
                     /locus_tag="IW774_002531"
     CDS             3175..>3444
                     /locus_tag="IW774_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1154854.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEK"
BASE COUNT         1298 a          424 c          654 g         1068 t
ORIGIN      
        1 tatcagaaaa atgtaccaga aatatttaaa aacggatatt atttatatta cgaatttaga
       61 gccggaggaa tacctgacta tattaaaaga gtgcaaattt atgatcggaa attcttcttc
      121 tgggataatt gaagctgcat cattcaaaat accagtaata aatttaggaa ccagacaaaa
      181 tggacgagaa caatctaaaa atattataaa tgcaaatcta aatgaaaatg aaattgaaac
      241 tgctttgaat aaaatacaag atcctcagtt tataaaaagc ttgaaaaatg ttaagaacat
      301 ctacggtgac ggcaacagtt ctagaagaat aacaaaaaca ttgaatcaaa tattgaatga
      361 tggggaagat attaagtgga ttcaaaaaca aatagcttat taaaatatac tagtgatcca
      421 tacattatta ttggggctgg cggacacgca aaagtcatta ttgaaacctt aagattagaa
      481 aataagttaa tatatggatt ttttgatgat aagcaaaata aaatgcaaga tctgaaatat
      541 cttgggaaaa taaacgaatt gaatagctgg gcggatagca aaaaaaaaat ctataaattt
      601 attgtagcta ttggtgactg tgtagtaaga aaaaagatta taaaaaaatt gaatatgcca
      661 agtgagcaat atggaatagc tgttcatcca agtgctatag tggcaaaaga aatttcaatt
      721 ggttatggaa ctgttatttt tgcgaatgct gtaattaacc cagatgctgt aattggagag
      781 catgctataa taaacacagg tagcattgtt gagcatgatt gtagaattaa taactatgtt
      841 catttatctc caggtgtatg cttagcgggt ggagtacatg taggtgtagg aactcaaata
      901 ggaattggtt ctcaatgtat ccagatgaag aaaattggat cttggtgtat gataggcgca
      961 gggagcacta ttgttaagga tattccctca cattccttag cttatggcaa tccagctaaa
     1021 attaagaaag aaggaattaa ttttgaattc tagaatttta ttatcaagtc ctcatatggg
     1081 aggaacagaa gaaaaatatg ttgctaaggc ttttaaagat aattggatag cgccattagg
     1141 tgaaaatgtg gataaatttg aagaaagtat tacaaaatat actggttcta agggcggagc
     1201 ttctgcgtta agttcaggta cagctgccat acatcttgcc ttaagactgc ttaatgttgg
     1261 aaaaaacgat attgtctttg ctccttcatt tacttttata gctacagtaa atcctatttt
     1321 atatcaaggg gctgtaccag tgttcattga ctcagaacct aatacatgga atatgagccc
     1381 tgttgcacta gagagcgcat tagaagaagc tgaaagaaac aaccaattac ctaaggcaat
     1441 catcgtagtg aatttatatg gtcaaatggc tgattatgaa cgaattttag ctattgcaaa
     1501 ccaatttaat gttccagtga tagaagatgc agcggaatcg ttaggttcta cttataaggg
     1561 aatttcgtca ggcttatttg ggacaattgg gatctattca tttaacggta ataagatcat
     1621 cacaacctca ggtggaggag ctctagtgtc tagtgataaa aaatatgttg agcattcaag
     1681 atttttagca acacaagcaa gagatcaagc accgtattat caacatagcg aaataggtta
     1741 taattaccgt ctaagcaata tttctgccgg tatcggacgt ggacaaatgg aagtattgaa
     1801 tgatagagta gctaaacgaa gagaaatttt caatcgatat aaggaagctt tttctggaat
     1861 tgatagaatt gaaatgatgc cagaagtgga tggcatggtg ccaaatcgtt ggttgtctac
     1921 tttaacgatc aatgattata attctattga tcaagtagtt aacgtaataa ataaactaaa
     1981 tgaaaaaaac attgaaacaa gagctttgtg gaaaccacta catttgcaac cagtatttca
     2041 aggggtaaaa atgtattacc atgataataa tcttagtgtg tcagaaaagt tatttaatag
     2101 cggcttatgc ttaccttctg gaagtaatat gacagatagt gaacaagaat ttgttattga
     2161 tgagtttaaa aaaatattca agtaggtgtg atattttatg aacataggaa ttattggttt
     2221 aggttccatg gggaaaagaa gacttagact cattcaagaa cactttaatg atattttact
     2281 atgtggtgtt gatactaatg aggcgagacg aaaggaagtt gaaaaggaat ttggaatacc
     2341 aacttataat agtctaacag aggcaaaaaa tttgtttgaa ataaattcag tttttgtttg
     2401 tacatcccct ataacccatg aatttattat aaaagaggct ttaaatttag attgtaatat
     2461 ctttacggaa atcaatttat taaatgaata ttatgatgaa ataatcgaag tagctaaaaa
     2521 gaaagataga ttactttatc tatcttctac atttttgaag agaaaagaaa ttcaatatat
     2581 taagtctaaa gtgacgcata ataaaaaaat ttcttatcaa taccatgtag gacagtattt
     2641 acccgactgg catccttggg aagattatcg caattttttt gtttcaaaca aaaaaactaa
     2701 tgggtgtaga gaaatattcg ctattgaatt accttggatt atagatacat ttgggaaagt
     2761 taaatcgttt actgtaatga cccagaaatt atccaatctt gatattgatt attcggacag
     2821 tttcactctt attattgagc atgaaactgg tatagttgga actttaagtg tagatgttgt
     2881 ttcaagagta gctaaaagaa atttaactat aattggtgaa aagattttaa tagaatggga
     2941 aggtaacccg gattctctat tttcatggaa tgaagaaact tctagtatga cagatattca
     3001 attgtacgat gaggttatgc atgataataa ttattcacat actattattg aagatgcata
     3061 tttagaggag attaaagaat ttttcacgct tttggaaaag aaaatggaac cccaatatac
     3121 ttttgaaaaa gataagtata ttattaactt aattaacgac attgaggtaa attaatgatg
     3181 aaagtaattt tttttgggtt agggtcaatt ggaaagagac atctaaaaaa tctaataagc
     3241 tatggaaaaa agaattctat taattttgat ataacagctt atagtagcag tggaaaaaaa
     3301 gagtttgaag aaataacgta tattcaaaat agagacgatt tggatacaca ttatgatata
     3361 gcttttatta caaatccgac ttttaagcat ctggaaacac taaatgtaat tgttgaaaaa
     3421 gttgattaca tatttttaga aaaa
//
LOCUS       DACEOE010000210         3440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEOE010000210 DACEOE010000000
VERSION     DACEOE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.216"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..206
                     /locus_tag="IW774_002532"
     CDS             <1..206
                     /locus_tag="IW774_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1154855.1"
                     /translation="SQRGLNEHSNGLLRKDGLPKEMDFNQVNQGFISSVASKRNHIPR
                     KSLNYQTPLEVFLSYVNGKFCLA"
     gene            184..1659
                     /locus_tag="IW774_002533"
     CDS             184..1659
                     /locus_tag="IW774_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1154856.1"
                     /translation="MESFVSLNLTNNTSKKNQPKDIKDIVDTRMYKERDDLNYTLIIN
                     KDGNYSWRPLVLLHPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASK
                     GSKRTDLGETILNWWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEK
                     VKNDENKCSGSLGDKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELK
                     KLENLKDYKVLRYRDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIII
                     DSIKKDKLYWEEIKASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKK
                     ALSEFNKERIIKITSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAR
                     EIVEMIINKYALKPNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVD
                     WLNEDAKKSFYEGTIKDEEKYIKMSKKIYITEFDDFNDYPF"
     gene            1997..>3440
                     /locus_tag="IW774_002534"
     CDS             1997..>3440
                     /locus_tag="IW774_002534"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1154857.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1266 a          553 c          616 g         1005 t
ORIGIN      
        1 cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaagat ggactaccaa
       61 aagaaatgga cttcaaccaa gtcaatcaag gattcatctc atccgttgcg tccaaaagaa
      121 accatatccc tagaaaatca ctaaattacc aaacaccatt agaagttttt ttgagttacg
      181 taaatggaaa gttttgtctc gcttaatttg acaaataata cttcaaaaaa aaaccaaccg
      241 aaagatataa aagatattgt agatacacga atgtataaag aacgtgatga cttaaactat
      301 acgcttatta tcaataaaga tggaaactat tcatggcgtc ctctcgttct actacatcct
      361 attctatatg tagatttggt taattgtctc actaaagaag accattggga ggagttaaaa
      421 aagaggttta gtgaatttaa aaaagataaa aatattaact gttattctat acctgttgaa
      481 gcttccaaag gttcaaaaag gacagatctt ggtgaaacaa tactaaactg gtgggaatat
      541 tttgaacaaa aaagcttagc tcagaatatt aaataccaat attgcatgtt tactgacatc
      601 gctgattgtt atccatcaat ttatactcac tctataacat gggccatgta tggtaaagaa
      661 aaagttaaaa atgatgaaaa taagtgttcg ggctcacttg gcgataaaat agataaaaaa
      721 atatctggaa tgcaacacaa tcaaacaaat ggtatccctc agggttccgt tcttatggat
      781 tttgtagcag agatcgttct agggtatgtt gatttgagat tatcgaaaga attaaaaaaa
      841 ttagaaaatt taaaagatta caaagtactc agatatagag atgattatag aatattttca
      901 aataatcgta atgatttgga acaaattatg aaagtgttat cagaagtctt atatgattta
      961 aattttaagt taaattctag taaaacaaaa ttatcagatg atattatcat agattctatc
     1021 aaaaaagata agctctattg ggaagaaatt aaggcaagta taagaatgaa tgccacaaac
     1081 ttagatggtg atagagaaat tcgttatagt ataaatttac aaaaacattt attggaaata
     1141 aaaaagttag cagataagca tcctaattca ggccaattga aaaaagcact ttctgagttt
     1201 aataaagaac gcattattaa aatcacttca aagccgaacg atcttttacc acttattggt
     1261 atcctggtag atatcatgat aaataacccg ggttctttgc cgcagtgtgt cttaattata
     1321 gggcagttat taaatttcga aaatgagagt aaggctagag aaatagttga aatgattata
     1381 aacaaatatg ctttaaagcc aaatactgaa tttatgatga tatggtttca aagattgacc
     1441 accatttttg gagaaatgtt tgatagtgtt ttatcatccg aatttgtatc taaaatcaaa
     1501 gatcctgtct ctaatcagat gttttctgta gactggctaa atgaagatgc taagaaatct
     1561 ttttatgaag ggaccattaa agacgaagag aaatatatta aaatgagtaa gaaaatatat
     1621 attactgaat ttgatgattt taatgattat cctttttaag tgaaaattta gaatgaatat
     1681 aaaaaacatt acaatctaat ttttttaaaa aaatgttata tcggaagtaa tctcttgatt
     1741 tctatactat aagataagaa aaccactatt taacaaggtt attcgaacct tattttaagt
     1801 tacttattat ttaatgtatc gaaaaaggtg aaataggttt gttataataa gaattaagta
     1861 agacatgaat tgtctaagca aatgtagctt atccatttca ttaaaataca tatggtgaaa
     1921 aaacaagttc aaatagtaac taatttttta aaaaactgat ttataaaaaa tacaacacga
     1981 atgaggaaaa taaccaatga cagaacaaat cgaaaaaaat tcaaaaagct tatatcaggc
     2041 actgtggaat tccgcagata tcctgcgttc aaaaatggac gctaatgaat acaagagcta
     2101 tttactaggc ttggtctttt ataaatactt atccgacaac atgctgcggt atgtctccgt
     2161 tctattggag gaagaaacgg acgacctaaa taaagcacaa gacctttacg ttgaagcatt
     2221 taaagatgca gatataaagg atgatctctt gtatgaacta aaagatgagt tttcatatac
     2281 catcgcaccc gcattgacct ttaccgcgca agtggcagcc attcatgatg gatcttttca
     2341 attagaagac ctggtacaag gtttccgtga cattgaacaa tccagcgaaa actttgagaa
     2401 cttatttgaa gacattgatc tgtattctaa aaaactgggg tcaaccccac aaaaacaaaa
     2461 taaaaccatc gcagatgtta tgaaagaatt agaagggtta aacatggcgg ggcatgctgg
     2521 cgatgtctta ggcgatgcct atgagtactt aattggccaa ttcgcatctg aatccggtaa
     2581 aaaagccggt gaattctata ccccacaacc ggtcgcaaaa ttgatgacac aaattgtttt
     2641 acaaggaaaa gaagataaaa aaggcttttc tgtttacgac gcgaccatgg gttcgggctc
     2701 gttacttttg aacgctaaaa aatattccca tcaaccggga accatttctt atttcggaca
     2761 agagttgaat acgtctacct ataatttggc gcgtatgaac atgattttgc atggggttcc
     2821 cattgccaac caacacttgc ataatgcgga taccttggat caagactggc caacagaaga
     2881 gccaacgaac ttcgatgggg tgctcatgaa cccaccgtat tctgcaaaat ggtcggctga
     2941 taaaggcttc ttagatgacc cacgcttctc tgcctatggc gtattagccc ctaaatcaaa
     3001 agcggacttt gctttcttat tacatggcta ctatcatttg aaagatacgg gcgtcatggc
     3061 cattgttttg ccacatgggg tgctctttag agggggcgca gaagggaaga tacgcaaagc
     3121 cttgttagaa aatggtgcca ttgacaccgt cattggctta cctgctaaca tcttctttaa
     3181 tacgtccatt ccaacgaccg taattatctt gaagaaagac cgtgcgaaaa aagatgtgct
     3241 ctttattgat gcgtcacaag attttgaaaa aataaaaacg caaaatacgc tacgagatta
     3301 ccatattgac gctattttag aggcctataa aacccgtaca attaaagaca agtatgctta
     3361 tgtcgcagaa tttgatgaaa ttgtggagaa tgattacaac ttaaacattc cccgttacgt
     3421 ggataccttt gaagaagaag
//
LOCUS       DACEOE010000211         3398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEOE010000211 DACEOE010000000
VERSION     DACEOE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.217"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..276
                     /locus_tag="IW774_002535"
     CDS             <1..276
                     /locus_tag="IW774_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ1154858.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="IW774_002536"
     CDS             419..640
                     /locus_tag="IW774_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1154859.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="IW774_002537"
     CDS             656..1498
                     /locus_tag="IW774_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154860.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="IW774_002538"
     CDS             1561..3363
                     /locus_tag="IW774_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ1154861.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1228 a          469 c          703 g          998 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actgctggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaata
//
LOCUS       DACEOE010000212         3374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEOE010000212 DACEOE010000000
VERSION     DACEOE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.218"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            254..580
                     /locus_tag="IW774_002539"
     CDS             254..580
                     /locus_tag="IW774_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154862.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(697..1041)
                     /locus_tag="IW774_002540"
     CDS             complement(697..1041)
                     /locus_tag="IW774_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154863.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1117..2364)
                     /locus_tag="IW774_002541"
     CDS             complement(1117..2364)
                     /locus_tag="IW774_002541"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1154864.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IW774_002542"
     CDS             complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IW774_002542"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1154865.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          987 a          666 c          670 g         1051 t
ORIGIN      
        1 ccgtttacca agaggttgtg agactgtcgt ttaactctga atcacaagga acaatttcaa
       61 aaaacagttt ctcatgtttg ttgaatattg tgacttatga ttgaggagtt ggcagaagaa
      121 caccatttat tcggaaaaag gggatgtgac acgatttttg tcacattccc tttttttctc
      181 aaaataatga aactggaaaa ttttcagtag gtaagctaaa attcatttaa gaaaaagaat
      241 ggggggataa tagatgctgg aagaatattt tcaggaagtc gggttgacac tcgttgaatt
      301 acccattgaa ttttcagatg atcggttgat ttatcgtggc tatatcaaag aattgccaat
      361 aatcaatgcg gaaggtgaaa ctagaaaagc gatgcagcaa aaaatagctg gtatgtatca
      421 ggtgtatcgg gaacagctac tggctgaatc agcggaagaa gaaaaagaaa aaacgatgaa
      481 cttgtccatg gatgagctgc ttcgttatta tgatggcgaa acatttgacg gattttcgct
      541 tcttaacgaa gaggaaacag atttaccagg cagctggtag gcgaacgccg tttatatgga
      601 aaaagaggct gtgacaaaat ctttgtcaca gcctctaaaa agtccggcag ttttttgccg
      661 atcttcaagg cactctactt atcgtacgga tattttttag cggaaagtcc gattcaagtg
      721 gtttttattc agctgatatt tataagataa gtgaaaaatc ccagcaatca gtagcgtaat
      781 cgtataagcg attgaatgat cgaaaaaaga atagtaattg attgtcgcta ataagcataa
      841 tgcaaccatg taactaatgt gcaagcgtgt aaccagccct ttttgcccga ttttgtcaaa
      901 atactgcgac catttttccc gttgactccc tatgcttcgt tttttgaatt gaaagaaaag
      961 ggataaaata ccgactccca gaaaagcagc gatattcaga gaaataaggt aaataaaaaa
     1021 aatcattaaa atttcaaaca tattgacacc tttctagttg gatagaacct ttacagaaaa
     1081 aaggcaaaag gcgggaataa tgtttcacac gaaacattat tcgcggattt gtccatcgcc
     1141 ataaatgata tattttgtgg aagttaattc atttaacccc attggaccac gggcatgcaa
     1201 cttttgagta gagataccaa tctctgcacc aaaaccaaat accgatccgt ctgtaaaacg
     1261 agtggaggca ttgacataga cagccgccgc gtccacttca tttaagaaac gttgactagc
     1321 cgcataacta tctgtcacga tcgtttctga atgtttagta ttatgccgat tgatatgtgc
     1381 aattgcttca tccaaagaat cgaccacttt gacagcaagg atataatcta gatattcagt
     1441 atcccaatct gtgtcttctg ctaaagcagc cgtctctaga tattctaacg cacgagcatc
     1501 cgcacgtaaa gagaccccgt gttctaccag ttctttttca atagctggca agaaaaaagg
     1561 tgcgatctca gaatggatca gcaatgtttc tgccgcgtta cagacagatg gacgggaagc
     1621 ttttgcattg acgatgatgt tcactgccat agataactgt gcttctttat cgatatatac
     1681 gtgattattt cctgtacctg tttcaatgat aggtaccgtt gcctgttctt tgactcgttg
     1741 gattaatttt gctccgcctc gaggaatcag cacatctaag tattctgtca atcgcatcaa
     1801 ctgtcctgca gtttcatgag aagtatcatc gacaaactgg atggcataag gagattcgcc
     1861 agcactgacc agtacttgct gcaataattg gactaataat tgattcgaga agaatgcttc
     1921 tttgccaccg cgaaggataa cggcattccc tgatttgaat gctagactag ctgcatctgt
     1981 cgtgacattt ggacgagatt cgtaaatgat gccggtcacg cctagtggca cgcgcttttt
     2041 gccgattctc aatccgtcgg tgttcgtcca catctcatct acttttccaa taggatcgtc
     2101 taacgaagca attttttcga tctctgtagc catggcattg attcgctcag gtgtcagctg
     2161 caagcggtct tgcatcgttt cactgatatt gttttcgact gctttttcta agtctttatg
     2221 atttgcttca atgatcgctt gttgattctt tttgatggtt tgtgccattt gaacaagtag
     2281 ctcatttttg cggtttgtag gcataagtgc tagttgttgc gcgctatctt ttgcctgttg
     2341 tcctaattgc atcaagtcag tcatttcaag tcctccttaa ataaagtacc tacttctttt
     2401 cctgcaagaa tatcaaaaat aattgccgga ttttttccat ttgctaaaat cattgcgctg
     2461 tttgcttcaa atatcctaga agcggctttt aatttactct gcataccgcc tgttccaaat
     2521 gtgctaccag caccacctgc ttgtgccaga atcttttcat cgattttatc gatatagcga
     2581 tacatctgag cctcgggatt tgtagtcgga ttatctgaat aaaacccgtc gatatcagaa
     2641 aggacgatca aaaggtctgc cgcgacaagt tttgccatga tggctgacag ctgatcatta
     2701 tcgccaaatt ttgtcagatg atctaattca tccactgcta cagtatcgtt ttcgttcacg
     2761 atagggatcg tgtccataat caaaagctgt tccaatgtat tcctcacatt tttacgactt
     2821 tcaggataat ctaccacgtc tcttgtcacg aggatctgtg ccgtctgttg attatataca
     2881 ctgaatcttt gattatagat attcatcagt tcggcttgac caacagccgc cgcagcttgc
     2941 tgttcaggaa tcaaagtcgg acgttctgac aaatgtaatt tatgcatacc aacgcctaca
     3001 gctcctgatg agactaagat cacttccttt ccccgatttc taagatcaga aaggacaaaa
     3061 gctaactgat cgattgccgc aagattgata tttccattgg gataaatcaa tgtacttgtt
     3121 cctactttga caataattcg ttttgcattt ttaatttctt tacgcatgtg acacctcaat
     3181 caaatatcca ctcattaatt tcgttctatt ctacttgttt tgacgagtaa agtcatgtaa
     3241 aaaatagttt ttttgtacaa cttgcagtcg tttggaaaaa gaacgaatta atggtttact
     3301 ttaagatagc agaatttctt gaagaatttg taaagaaagt cttttacagc ctcggatatg
     3361 ttagaatagg agta
//
LOCUS       DACEOE010000213         3333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEOE010000213 DACEOE010000000
VERSION     DACEOE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.219"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(350..1390)
                     /locus_tag="IW774_002543"
     CDS             complement(350..1390)
                     /locus_tag="IW774_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1154866.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1739..2065)
                     /locus_tag="IW774_002544"
     CDS             complement(1739..2065)
                     /locus_tag="IW774_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154867.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2052..2840)
                     /locus_tag="IW774_002545"
     CDS             complement(2052..2840)
                     /locus_tag="IW774_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1154868.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1019 a          537 c          450 g         1327 t
ORIGIN      
        1 actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa aagaagtaat
       61 atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat tattataact
      121 caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa taactaaaat
      181 atctaacgat tatgacttta aaacctacgt ttgtgattgt tttcataaac ttttaatcta
      241 aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa aatcataaaa
      301 aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac tactctagcc
      361 aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg tttgcaagct
      421 ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt agattctgca
      481 aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc atatataatc
      541 ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact gtttgctcta
      601 attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga gcgtcttcgt
      661 aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat gtatttatac
      721 tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt aactgatcag
      781 tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat agattgtctg
      841 cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta tttgcgattt
      901 tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg attttcgcaa
      961 ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct tgattcgtta
     1021 cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg tcacgtttat
     1081 ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt ttgattttag
     1141 atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg aatatgaaaa
     1201 atatattatc atcagaatct acccagttat ttttgactga ataatcaaat cgatctttaa
     1261 gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg ttagtaaaaa
     1321 ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg taataattaa
     1381 aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac tatgttacaa
     1441 tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa gtcgccaaac
     1501 tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa ataaattaaa
     1561 gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata ttacaataat
     1621 attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca gctttgatac
     1681 tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg aatgtaaatt
     1741 aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct tcaggtatgg
     1801 actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca gcaatagtcg
     1861 tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct gctgttctta
     1921 cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat tcgatttctt
     1981 ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta cttactgcag
     2041 cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata ttcttctgta
     2101 aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag atctgatcca
     2161 gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa aatttttgca
     2221 ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat gttataacgc
     2281 ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt atattctttt
     2341 accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata cgcagcttca
     2401 actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat tcctaaactt
     2461 ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag tactctttta
     2521 agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag ggctatgtcg
     2581 gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc agcctcccct
     2641 tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc tacattatct
     2701 gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta aacaaatttc
     2761 aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt ttgattggct
     2821 actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata atataattat
     2881 atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat tgtgtgatta
     2941 ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt tattcgaaat
     3001 taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa tatgttttgc
     3061 gagcagcaac tagaataatt taaacattac taaactgtct atattgttac taataattcc
     3121 cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat attaagatat
     3181 cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt tatcatgttt
     3241 aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa catcagacat
     3301 gtttaaacta gaggccaggc ttaaatcata aac
//
LOCUS       DACEOE010000214         3296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEOE010000214 DACEOE010000000
VERSION     DACEOE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.221"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            205..1836
                     /locus_tag="IW774_002546"
     CDS             205..1836
                     /locus_tag="IW774_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1154869.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSNIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1839..2918
                     /gene="uxuA"
                     /locus_tag="IW774_002547"
     CDS             1839..2918
                     /gene="uxuA"
                     /locus_tag="IW774_002547"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1154870.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLQTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2992..>3296
                     /locus_tag="IW774_002548"
     CDS             2992..>3296
                     /locus_tag="IW774_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1154871.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNP"
BASE COUNT         1169 a          437 c          680 g         1010 t
ORIGIN      
        1 atacatgaaa aaaagaaaaa aataagtttc gataaaatca gtccaaaaag aaggagtatt
       61 ttatttttct gaataaaaaa tacaataatt ctgatatggt agaaaaaaag caagtttatt
      121 ccatttttat attgacaacg gttacaacgg gttttaaaat ataaaatgta tcaaaactaa
      181 catattagtt taaggaggtt aattatggtt tcgattaaaa attggcaaaa ttctatcgaa
      241 gaattcaatg aaatgcagat tctactgccg tgttttgatg tcaaagaagt taagcaagaa
      301 ggccagcaac atccagtatg gcttcatttt ggtggaggaa atctttatcg aggatttcat
      361 gcagaaattg ctcaatgctt agcagatcaa aatgaattag aaacaggagt agttgtgtgt
      421 gaaacatttg atgaagaagt agtggaaaaa gcttatcatg actacaacaa tgacattttg
      481 gaaattgtta tgagtggaga tggtgaactc aaaaaaaggc ttttggcagt aacggctgat
      541 agtttatatt gcaatccgaa gaatatagcg gactatcaaa gagtggttaa atattttgaa
      601 gatcctaaat tacagtttac aacgtttact attactgaaa aaggttacag tttaaaagat
      661 tctaaagggc aattcttttc ggtagtcaaa gctgatatca aatctggacc tagcaatcct
      721 caacatacga tgagtattgt aacatccttg ttatatagca gatttaatgc tggagagttg
      781 ccgattgcga tggtttctac tgataatttc tcacagaatg gtcaacgttt tcaagatagt
      841 atttttacga ttgcagaagc ttggcaaaaa gaaggatatg tttctgaaaa atttattgct
      901 tatttaaagg ataaagaaaa agtgactttt ccttggagta tgattgatcg aattacgcca
      961 aatccttcag agaatgtaaa ggaagatcta ttgatgatgg ggctcgagga tttagatatc
     1021 attcacacaa aaaaacatac caatattgct gctttcacta atactgaggt agttcattat
     1081 ttagttattg aagatagttt tccaaatggc cggcctaaat tggaaaaggc tggtgtgatt
     1141 atgacagata gagagacggt tgataaagca gacatcatga aggttacgac aagcttgaat
     1201 ccacttcata ctgctttggc agtaacaggt tgtctgctag gatatcaatc aatttcagca
     1261 gagatgcagg atgatgattt ggttggttta atcaaaggaa taggttattt agaaggttta
     1321 ccagtagttg aaaatccagg cattattgat ccaaaacaat ttattgatga agtaattaat
     1381 aaacgtttgc ctaatcccta tattccagat acaccacaac gaattgcaac agatacttct
     1441 caaaaagtag cgattcgcta tggtgaaaca attaaaaagt atgtcgaaaa agacggaaat
     1501 gcagaaaatc taacatttat tcctatcgct attgctgctt ggttacgtta tttactggca
     1561 gtcgatgatc aaggggaaaa ttttgtgcca agtcctgatc cgttattggt agagttgcag
     1621 gataaattat cagtattgaa attaggtgaa caagatcctt caaatattca ttgtgcggta
     1681 caggaaattt tagaaaataa agaaattttt ggtttagatt tatatcaagt tggggtagga
     1741 aagaaaactg aaaaaatctt ttgcgaaatg ctagtaggtc ctcaagcagt tcgtaaaaca
     1801 atcaataaat atataacaga agttggagga aagtaactat ggaaatgact tttagatggt
     1861 atggggcaaa tgaagaaaaa atctcactac aagaaattcg tcagatccca gcaatgaaag
     1921 gaattgttgg aacattaatg gatataccag ccggagaggt ttggccgaga gaacgaatta
     1981 aatctttaaa agatgaagtc gaagccaatg gacttacctt gaaagttatt gagagtgtta
     2041 atgttcatga tgatatcaaa attggtttac aaacacgaga tcaatacatt gagaattata
     2101 aacagacgat tcgcaatttg gcagaatttg ggattgaggt tatctgttat aattttatgc
     2161 cagtatttga ttgggtaaaa tcggatttgg attatcgttt agatgatggg tcttctacgt
     2221 tagcctatat ttcaaaaaat atccctactg atccggagga aattattaga aaagtagaaa
     2281 attctactaa taattttgaa ttgccgggtt gggaacctca tcggttatca caaattaagc
     2341 aattattaga agcctataaa gaagttgatg aagagaaatt acgagagaac tttgcatatt
     2401 ttctcaaagc aattatcccg acatgtgaag aagttgggat taaaatggca gttcacccag
     2461 atgatccacc atattccatt ttcggattac ctcgtattgt taaaaatcgt gacgatttag
     2521 attggttgtg taatgtagtg gattctcctt ctaatggtat tacattatgt actggaagta
     2581 ttgcagaacg accagataat gatgtctatg aaattctatc tgaattttgt caacgggatc
     2641 ggattccttt tgcacatgtt cgtaatatca aatatttaag tgacaatgga ctcgattttt
     2701 acgaagcgcc gcataaatca gaatatggtt ctctagatat gtataagata ttaaaaacaa
     2761 tgtacgataa tggatttaat ggctatattc gtccagatca tggtcggatg atttggggag
     2821 aaaccggtag acctggttat ggattatatg accgagctct aggtgcagtt tatttaaatg
     2881 gtatttggga agcgcttgaa aagggggcga gtaaataatt atataatgga gtttatttag
     2941 aggaaaaatt ttctcttcta aaaactaacg gaaggaaagg agtgtttaaa tatgttttta
     3001 gataagaact ttttattaga gaatgaaata gctaaaagac tttttcataa atttgcaaaa
     3061 caccaaccaa ttatcgacta tcattgtcat ttagacccag catatttgct agaaaataag
     3121 caatacaaga atttgactca ggtatggtta aatgataaag gagctggaga tcattataaa
     3181 tggcgattga tgcgagctaa tggtatagaa gaagagttga tcagtggtcc aggtgatgat
     3241 tatgaaaaat ttttagcttt tgtaaaaaca gtggagaagg cgccaggtaa tcctat
//
LOCUS       DACEOE010000215         3292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEOE010000215 DACEOE010000000
VERSION     DACEOE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.222"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            37..588
                     /locus_tag="IW774_002549"
     CDS             37..588
                     /locus_tag="IW774_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ1154872.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1530
                     /locus_tag="IW774_002550"
     CDS             1264..1530
                     /locus_tag="IW774_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154873.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1588..2100
                     /locus_tag="IW774_002551"
     CDS             1588..2100
                     /locus_tag="IW774_002551"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1154874.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="IW774_002552"
     CDS             2097..3134
                     /locus_tag="IW774_002552"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1154875.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1213 a          555 c          653 g          871 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac ac
//
LOCUS       DACEOE010000216         3230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEOE010000216 DACEOE010000000
VERSION     DACEOE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.224"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            35..1336
                     /gene="murA"
                     /locus_tag="IW774_002553"
     CDS             35..1336
                     /gene="murA"
                     /locus_tag="IW774_002553"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1154876.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1349..1525
                     /locus_tag="IW774_002554"
     CDS             1349..1525
                     /locus_tag="IW774_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1154877.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1660..1956
                     /gene="yidD"
                     /locus_tag="IW774_002555"
     CDS             1660..1956
                     /gene="yidD"
                     /locus_tag="IW774_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1154878.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2141..2608
                     /locus_tag="IW774_002556"
     CDS             2141..2608
                     /locus_tag="IW774_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1154879.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2693..>3230
                     /locus_tag="IW774_002557"
     CDS             2693..>3230
                     /locus_tag="IW774_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1154880.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYC"
BASE COUNT         1088 a          523 c          723 g          896 t
ORIGIN      
        1 tgtatgctca ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg
       61 caatcaatta aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt
      121 agctgctagt ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc
      181 tgatgtcttc actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga
      241 acaaaagaat caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga
      301 gtatgttagt caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg
      361 acatgcaaaa gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca
      421 tttaaaagga tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc
      481 aattgcggat gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac
      541 tcaaaatatc atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc
      601 tcgcgaacca gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta
      661 tggagctggt actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca
      721 ctcaatcgta caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca
      781 aggcaatgtg ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt
      841 gatcgaaatg ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa
      901 acatatctta cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca
      961 agctcaaatg acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt
     1021 gtttgaaaat cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat
     1081 cgacggaaat gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc
     1141 aacagacttg cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac
     1201 acgtgtgcgt aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca
     1261 gcaattaggt gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc
     1321 acaaacgatt gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt
     1381 gataaagatt ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat
     1441 cggatatggt gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat
     1501 tcatattcga gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa
     1561 gtgagccatg aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg
     1621 atcccttttt ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat
     1681 ttagttagag gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac
     1741 ccaacctgtt ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg
     1801 gtaatgggga ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat
     1861 gtaccattga cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa
     1921 aaggttgtga aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc
     1981 ttatattttg caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa
     2041 aaaggttgtg aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc
     2101 aaagtaacaa ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa
     2161 gaaagattta ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt
     2221 atatgtttcc agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact
     2281 gatctatgtg gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta
     2341 tcaaggaaag gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact
     2401 cgcttccagt caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca
     2461 tctctatcaa aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca
     2521 ttactctgag cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat
     2581 tcacttacct aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat
     2641 gctaaataga tacattacaa tagctttagc aaacaaaaag gagaatattt ttatgggaat
     2701 attagttttt gattttggtg gatcggctgt caaatatggt tgttgggatg gcaaagagat
     2761 taaaggtaag ggacaatttg caacgcctga atcttgggaa gaaatgaaag cgcaattatt
     2821 tcaagtttat aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc
     2881 tggagtagtg aataatgaga aacaggtgat tgagggaatc agtgcgattc cttatattca
     2941 tggatttaat attttccgag atttggaaac tctgtttcaa cttccagtaa cgattgaaaa
     3001 tgacgccaac tgtgccggaa tggctgaatt ttatgaaggt gctgcccgcg attatcaaga
     3061 tgttgctttt gttgtggcag gaactgggat tggcggggcg ttgttcacga aaggacaaat
     3121 caataaaggg gcacatcttt acggtggaga atttgggttg atgttcttaa atggcgataa
     3181 gacattcagt aaacttggta cagcggtaca gatggcttgg cgttattgtg
//
LOCUS       DACEOE010000217         3224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEOE010000217 DACEOE010000000
VERSION     DACEOE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.225"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(552..2165)
                     /locus_tag="IW774_002558"
     CDS             complement(552..2165)
                     /locus_tag="IW774_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1154881.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="IW774_002559"
     CDS             complement(2306..3214)
                     /locus_tag="IW774_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1154882.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1038 a          555 c          571 g         1060 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttct
//
LOCUS       DACEOE010000218         3215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEOE010000218 DACEOE010000000
VERSION     DACEOE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.226"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            204..1172
                     /locus_tag="IW774_002560"
     CDS             204..1172
                     /locus_tag="IW774_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1154883.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="IW774_002561"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="IW774_002561"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1154884.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="IW774_002562"
     CDS             2202..2810
                     /locus_tag="IW774_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1154885.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          955 a          615 c          778 g          867 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaagat gtaaggcaga gcgatattgc
     2941 ggtcattatc tgcgtgcgct gcggcaagat agcctgataa taagactgat cgcatagagg
     3001 ggtggtattt cacaccgccc attgtcaaca ggcagttcag cctcgttaaa ttcagcatgg
     3061 gtatcactta tgaaaattca tctacattgg tgataatagt aaatccagta gggcgaaata
     3121 attgactgta atttacgggg caaaacggca caatctcaaa cgagattgtg ccgtttaagg
     3181 ggaagattct agaaatattt catacttcca actat
//
LOCUS       DACEOE010000219         3131 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEOE010000219 DACEOE010000000
VERSION     DACEOE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.227"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            110..445
                     /locus_tag="IW774_002563"
     CDS             110..445
                     /locus_tag="IW774_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154886.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            442..1059
                     /locus_tag="IW774_002564"
     CDS             442..1059
                     /locus_tag="IW774_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154887.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
     gene            1075..1542
                     /locus_tag="IW774_002565"
     CDS             1075..1542
                     /locus_tag="IW774_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154888.1"
                     /translation="MLKHCRKGFCKVIKGKNAADLIFEWKESNLINVRGKNGSGKTER
                     VMASIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYEEVLFVWITEEDSNSKSAFFSEKNAFLHSI"
     gene            1558..>3131
                     /locus_tag="IW774_002566"
     CDS             1558..>3131
                     /locus_tag="IW774_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAQ1154889.1"
                     /translation="MIVSKKLARNLVSEGYDLRLVEKYQPSGGIKFDERYISTGDGYV
                     TSIAVIEFAEDVNSRWLTDIMAIPNTIATFDVCTADKEDLLHKINTSIGELDYRSTNE
                     NRTTDKTDALLDKYNLEDFAQEITKRGEVVKTVHARIFVYSDSLEELEKRISEIRKDL
                     ASVNHKAIVPQFRMKDEWMSLFQDGMEQESKLFAKKGLTVPAKNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFYSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVKKAGGVYVDLGGGDMVVNPLQIYATVMKKDQFGNDVVDESKSFQ
                     NHITKCSNQIRFLEPEIDTATVREYTLMLQDFYIHYGILPKEWREARDDNERNQLNTN
                     ICNLPNDSYPIYGEFLKFIENQQFKIKNATPERVRSIELIKLIVTDMCTTYPQIFNGV
                     TTMPNFDTTKIVYYNIDSISKMSAPVFHCQLFTALTTIWNSALQNGKIQKENFKKYGS
                     DYYKYAQDFMVLIDEC"
BASE COUNT         1163 a          399 c          603 g          966 t
ORIGIN      
        1 caagacgaag attcacttat gaagacaacg aattattcag agatacactt aatgatccta
       61 atccactttt cgataattta aattaaatta aaagccggca tgagtgatta tgccggcttt
      121 ttatgaggtg aaaatgatgt atagaaatcc caaaaatata aaacaagaga taaagctatt
      181 tttcttctat ttagtcgatt taggaatagt agccggaagc gtgatggcaa gtgtttactt
      241 tgcaaaatta atgccaattt caccaatgat gaaatttgca ctgtacttat tagcatttgt
      301 agtgggtgtt tggatgtgcg ttagaacgcc taatcaccct aaagagcgaa atattttaat
      361 gctgtattat ctctttaaaa tggatagaaa cagataccac gaagtagagt ttaaaagaaa
      421 tagtaggggg aaaattgaat tatgattgtt gctgaaagta atgaaaataa attaagtggt
      481 gtttttgaca aattcttaga actagttatt ccaaaagata aagatatgtg tgaactttta
      541 ccatataaaa aaatattgaa agacggaacg ttagtgacta atgacaataa gtatcaagcc
      601 ttttttaaag ttaaaacaac tgacttaact tctatttcag aagatggatt tatgcgtatg
      661 gtaaacaaac tcacttattt aaatcgagta tatttagaac catataaaat ttattcatta
      721 acttatccta ctgaaacagt aaatcaagcg atttattgga agcatagaat acaggaggca
      781 aagagaagtt tattgagtaa taatgatccc aatttagaaa cgtatctgaa gtggaatcta
      841 aagctagctt acgataatta tgaaagagtt gatctagtat cgactttacc agagttgatg
      901 ttcgttattt gtgtttatgg aaaaacacaa aaagaagtga aaaatcatgc cgatgtaatg
      961 aaacaggccg gtggtagaga attagattta caaagaatta ctgaagggga tttaatcaat
     1021 gtgataacta aattgatgaa tcccaatact tctttataag gcggtgctta ttatttgtta
     1081 aagcactgta gaaaaggatt ttgtaaagta attaaaggta aaaatgctgc tgatttaatc
     1141 tttgaatgga aagaatctaa tttaattaat gtacgtggga aaaatggaag cggaaagaca
     1201 gaaagagtga tggctagtat tgataattca aggcctagat caagagcatt agtaataact
     1261 gatgaaaaaa atcactataa aggcatacga acgatagaaa aaaaatattt aactaacaat
     1321 gatttaaaag tagtagatga tattttaaat atttatcctg attttaattt agttgttttt
     1381 gatgaaacaa ttcctgtgac agaaataaaa ggattctatc aaagcttttc caatctagca
     1441 aataaatatg aggaagttct gtttgtttgg attacagaag aagatagtaa tagcaagtca
     1501 gcgttcttta gtgaaaagaa cgctttttta cattctattt aaaaaggaga attgaacatg
     1561 attgtaagta aaaaactagc aagaaatcta gtaagtgaag ggtatgactt aagattagta
     1621 gaaaagtatc aaccgagcgg tggaattaaa tttgatgagc gttatatttc tactggtgat
     1681 ggatatgtaa cttcgattgc tgttattgag tttgcagaag atgtaaatag tcgatggtta
     1741 acggatataa tggcaattcc aaatactatt gcaactttcg atgtatgtac ggcggataaa
     1801 gaagacttat tacacaaaat caatacgtct attggagaat tagattaccg atcaacaaat
     1861 gagaatagaa caactgacaa aacagacgct ttactagata aatacaattt agaagatttt
     1921 gcacaagaaa ttacgaaacg tggtgaagtt gtcaaaactg ttcatgctcg tatttttgtt
     1981 tatagcgatt ctttagaaga attagagaaa agaattagtg agattcgtaa agatttggct
     2041 agtgtcaatc acaaggcaat tgttccgcaa tttagaatga aggatgaatg gatgtcttta
     2101 tttcaagatg gcatggaaca agaatcaaaa ttatttgcca agaaaggctt aactgttccg
     2161 gcaaaaaata ttggtggcgg tattccattt caccacaaga gtttgaaaga tccacgtggg
     2221 ttttatttag gaactacgtc aactggtggt gcttttatat ttgatccatt ttattctacg
     2281 aagcaaagac ggtcgtttaa cggctttatt ttaggaaaga tgggttttgg taagtcaact
     2341 ttaattaaac agctacagga aggcctacaa gcgaaaaatt gttttattcg aggatttgac
     2401 aagtcaggtg aatttgaagg catggtgaag aaagcaggcg gtgtatacgt cgatttaggc
     2461 ggtggtgata tggtagtaaa tccattgcaa atttatgcaa cagtcatgaa aaaagatcaa
     2521 tttggaaatg atgttgtaga tgaatcaaag agttttcaaa atcatatcac taagtgtagt
     2581 aatcaaattc gctttttaga acctgaaatt gatacggcaa ctgtacgtga atatactttg
     2641 atgttacaag acttttatat tcattatgga attttgccaa aagaatggag agaagcgaga
     2701 gacgacaacg aacgaaatca attaaataca aatatttgta acttgcctaa tgattcatat
     2761 ccaatctatg gagagtttct taaatttatt gaaaatcaac aattcaaaat taaaaatgct
     2821 actcctgaac gtgtccgttc tattgagcta attaagctta tcgttacgga tatgtgtaca
     2881 acttatccgc aaattttcaa cggagtaaca acaatgccaa actttgatac aacaaaaatt
     2941 gtttactata acattgattc tattagtaaa atgagtgccc cagtattcca ttgccaatta
     3001 tttacagcgt taacgactat ttggaacagt gccttgcaaa acggaaagat tcaaaaagaa
     3061 aactttaaaa aatatggcag tgattattat aagtatgcac aagatttcat ggttttgatc
     3121 gatgagtgtc a
//
LOCUS       DACEOE010000220         3123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEOE010000220 DACEOE010000000
VERSION     DACEOE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.228"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            198..1031
                     /locus_tag="IW774_002567"
     CDS             198..1031
                     /locus_tag="IW774_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1154890.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1133..2833)
                     /locus_tag="IW774_002568"
     CDS             complement(1133..2833)
                     /locus_tag="IW774_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154891.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
BASE COUNT         1106 a          518 c          619 g          880 t
ORIGIN      
        1 tttgaatact gtttagtgga gaggtcgtga aaaagctgcc ctgcaccaag tgataaaaac
       61 agccaaacaa aaaatagctc ctggtccatt gagcttatta ctgaggagtt agcaggagaa
      121 cactatttgc tggagaggtt gtgacaaaaa ttttgtcaca acccttttgt ttcgaaatga
      181 agacaaggag aaaaccaatg aaattcatga cactcaacac gcatagctgg cttgagccag
      241 aaccagagaa aaaattgcaa gaattagcag ataagatcct gttagaagat tatgaagtga
      301 ttgctttaca agaaatcaac caattactgg aatctgaaga agtagaacct gcgaagctaa
      361 tgaagttctg tccggtaaag aatcaagtac ctatccgtaa agataatttt gcctaccgtt
      421 tagttcagct tttaaaggaa cacggaaaag agtatttttg gagttgggaa atgagccaca
      481 tcggttatga taaatatgaa gaaggaaatg cactgcttac gaaaaaaaca ttggaaagtc
      541 aggtccttac cgtctcccaa tttcaaaaga aagaagatta tcagacacgc aagatcttga
      601 ttgggaaaac aaagatagat gatcaggaca tttttgtctc gagctgccac ttttcttggt
      661 ggacagataa aaaaagtggc ttttattttg agtggaaaaa cttggaaaac tactttttgg
      721 aaactcgcgt accacttttt ttcctaggtg atttcaataa tccagtagac tctcaaggtt
      781 attatactgt aagagaaagt tgccttcttc ttcaagattc gtatgttgtc gcgaatgaaa
      841 aaggaaaagc agctactgtg gaaaagaaaa tagatggttg ggaacaaaat acagaaaaat
      901 tgcgtataga ttttattttc gtccctgaag gaatgcaggt taaaaaatat caacggattt
      961 ttgatgggat cgatagtcca atcatcagtg atcactacgg ggtggaaatc gaattagatg
     1021 tgaacgaata aaaaatataa catataacat aaaaaaactg attaaaataa aactctccaa
     1081 acgattatac cgtttggaga gttttttgat cggatgtatt tatccctttt gcctaagcac
     1141 gtaaaagctt gtatctatga taaataaata aacatagcaa ggtaaaccag ccaatcaaag
     1201 taagagaaag aatgaaatag aaatagattg gcgtttgata atgaagcgtg agctcgttga
     1261 ttcctttttg ctgcttcacg attggggtcc cgatatctgt tacttcataa tcagtcaatg
     1321 tttttccatt ttgctgtagc acagtccgat cgtagacgat aacagggata tgaacagttt
     1381 tgtccccagt gtttttccac ttgattgtca aaagaccgcc tggatgtgtt tttataaaag
     1441 ttgtttcgtt ttctagtatt tcttcgttat accggtcgta tttattgtct tttgtctcct
     1501 tatagattgg cagatagtca ggtgtggttt tttggaaaac gaacaagctg gtagacatat
     1561 ctttgctgaa gaatgttttc cttgcttctt ccggcgtatc gaatagataa gtatgtcgac
     1621 tgacaaatgt ttcattttcc cacttttcca gatgctggta taagttctgg atcgtctgtg
     1681 ctgaaaaaat aagaatgcca aatatactta aaagacggaa ccacttttgt tcagaccact
     1741 ggttcagtga aagggcaagg taaagtagaa gcaatagcgt aaatggcacg aaaaatcgaa
     1801 atggaaactg gatcaaacta ataaaaggca catttttttc gatcaatgtc gtccatggaa
     1861 tcaagctagt agaaagaaaa aagaaaacaa gacttgtgta caacacgatt ttttggaata
     1921 aggtgttctt ttgttgaaag aaaataaaaa gcaaaaaacc tgataatatc aatataggaa
     1981 aggccttagg gtaatggagc caataactgc catccagatc tatcgccttt tgactcattt
     2041 ctctgttaac aaagggagga agaatcttat tcccactgta aacaatagct aatcctgccc
     2101 aaatatttgc tgtcagaaca aaataaagca agaccgactt aatcagttga agcagtgctt
     2161 ttcctttttc agtctggtta aaaaagtaat aaagataaaa agggatatac atcaaagcta
     2221 acatgatcga agacagtaaa tggatctgtg tcataagtcc cactgagaca gcgactttga
     2281 taacagggaa ctttttatga aggacgaaat cagcaatagg gatcatacat aaaggataaa
     2341 aacaagtccc ccagctagtg aatccttggt tgatagacca gtattgaata gaatacgtac
     2401 tcatgaataa aacggctact ggtacactta cccaatttcg aacattgaca tatctcagta
     2461 aaatcagcat agaaaatcct gaaataagat aaagaatgaa atttgaaaga atctgataat
     2521 gaaaccagct acctgcaagc aacacgatca atccttgtaa ataagcgaaa aatgggccgt
     2581 aaacggcatt cacgatcctg ccactttgct ggaatccata aagggaagga aaataatgga
     2641 aatccccata tttaatttgt tgcgcggcat catagaaccg attgtaatgg aatggggcat
     2701 cagcacccaa aattagttta tgtgtgaata aatgaggcat gagaaatgcc aaagcaatga
     2761 aaaaaattgc taaaaatggg ccatattttt tcaattgtcg aactcctttg caaactttag
     2821 ataaattcat catatatcct atgcgagagg taggcaagaa attagaaaat tcatgaaaat
     2881 atttatgaat tttacaagaa aaacataagt gaaagttcta aggagagagg atgcgacata
     2941 agttttgttg catctccttg ttttgaaaca gaatttttct taatactcga aacaaagtag
     3001 ctttttcatg acctctcggt aaacggtgtt caatcagctg cttctcggta ataagctcac
     3061 tagcctagaa aatatgagaa gctattttct gactagcgct cttattactc aaagcataac
     3121 gca
//
LOCUS       DACEOE010000221         3060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEOE010000221 DACEOE010000000
VERSION     DACEOE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.229"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(484..1098)
                     /locus_tag="IW774_002569"
     CDS             complement(484..1098)
                     /locus_tag="IW774_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1154892.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1704..2081)
                     /locus_tag="IW774_002570"
     CDS             complement(1704..2081)
                     /locus_tag="IW774_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154893.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2381..>3060)
                     /locus_tag="IW774_002571"
     CDS             complement(2381..>3060)
                     /locus_tag="IW774_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HAQ1154894.1"
                     /translation="ANIELENNLREKEKTELLALVEETTQVNLHFIRQSHPRGLGDAV
                     LQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRVPENETSKYGI
                     IDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILETQEPGAGNEIQ
                     LTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGTSLSAYIIQLS
                     KKLRTES"
BASE COUNT          955 a          618 c          459 g         1028 t
ORIGIN      
        1 aaaaataata aacacttata aatatccatt atctatcctt caatttttgt gtattttatt
       61 tctattagaa aaaggtatcc tgttggacaa ttttgttata tcaggtaaac ttcattgcaa
      121 taatgataac aaatgtacta taattacttt gtaaagagtt ttggggaaga ctctttcatc
      181 aacgtgtatt aagagacaag aaagaatcca tatggattct ttcttgtctc tttttccact
      241 actttttatt tttctgacac tacatattct tataactcat ttctcataac tgtgaaacaa
      301 cttataaaaa taacaaactt taaacaaaac aagcttaaaa tcaaagatga tccatcttaa
      361 aataactaaa acaaaaaaag tagtcgttgt taatcctcat taataatcgt tttctttttt
      421 tcaaacgctc catttttatg tggaacatta tttttttaag gaactttgtc aacatgtgga
      481 taactatttt ccacttgtgt cctggactag ctgttttgct gtaaaataca aaacttgctg
      541 cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc gctggatcta gatttggcaa
      601 agtcaagact gcatcatgta aattggcgtg gttgtccaaa accaaatcag caaattcaaa
      661 taatcgcaaa ccactgctat gttgcgattt gatacttcgt gacaaatcaa atccagtaac
      721 aatgattaat ggatgaccat tttctttgat ccactgagct agttcgatga ttgccgggtt
      781 tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa gctgatcaat
      841 aattgcctgt cctgcacctt ctagcgtttc atagacacct ccggctggat tttcaatcaa
      901 tatacttggt acaagatccg gggtttgatc ccaaaattca aatgctacac cacttagtgt
      961 gcggctagca tatatctgca atctaccacc ttgattaatt tgtttcgcga tccaagtgcc
     1021 agcttgaagc atcagcggct cttctatcct taatttctct tccaattggt gataatagtc
     1081 aaaataacat aaactcattt atctctcccg cctagctata cttgacatga atagaaacag
     1141 ctgcctaatc taaggaagta cataaaaaaa cagtagttag acagtaacac atgtggtctt
     1201 tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc caataccttg acatagcaaa
     1261 taaaacaacg tgcagaccat ggaaaatatc tacatagggg tagaattttc accctagatt
     1321 aggtacaata aattaggtcg atgcactgca ttgactatgg caaaatgatc ggtcagggtg
     1381 tatgtgtgtg actggtcatt ccctgattct tagcgccaac taagggtcag ggagcctttt
     1441 tttattcagc tgtttctatc tgaatttatt gtaagtaatc tcttatccaa gtgcaagcaa
     1501 gaaaatacaa aaaaacattg gtatgattta tatatatata aataacataa aaaaaggaat
     1561 ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata agtgaaaaaa
     1621 gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt attttatctt
     1681 tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact ttgttcgaga
     1741 gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg agactttctt
     1801 ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc aaatacgcaa
     1861 gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac ggatgctgca
     1921 taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat gataaataga
     1981 ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag ccacttgagt
     2041 atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac acaccttttt
     2101 ttataattaa aaaattcgcc tttttattct actatgaatc ttctggcttg gctttcattt
     2161 ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat tagcataata
     2221 ttttctgaat ctattcacga aagtcttttt ctatctttcg ttttccacga ctcaactgat
     2281 ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc cgctttaata
     2341 acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct gttcttagct
     2401 ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa tgttgaagtc
     2461 catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat ctttttcctt
     2521 taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca tccgttagct
     2581 gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt ttaggagtca
     2641 gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca ggtttttcta
     2701 caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca ataattccgt
     2761 atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct tgtgtttctt
     2821 cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa tcatccccca
     2881 gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg gcatccccca
     2941 gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt tcttcaacta
     3001 aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg atatttgcat
//
LOCUS       DACEOE010000222         3040 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEOE010000222 DACEOE010000000
VERSION     DACEOE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.230"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..226)
                     /locus_tag="IW774_002572"
     CDS             complement(<1..226)
                     /locus_tag="IW774_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1154895.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAI"
     gene            complement(220..621)
                     /locus_tag="IW774_002573"
     CDS             complement(220..621)
                     /locus_tag="IW774_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1154896.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(806..2614)
                     /gene="uidA"
                     /locus_tag="IW774_002574"
     CDS             complement(806..2614)
                     /gene="uidA"
                     /locus_tag="IW774_002574"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ1154897.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KLITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWKERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLQKRWSSIPNFSYKK"
     gene            complement(2637..>3040)
                     /locus_tag="IW774_002575"
     CDS             complement(2637..>3040)
                     /locus_tag="IW774_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ1154898.1"
                     /translation="SPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANV
                     NPGTVQRIVWPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQET
                     LEILVDKLMGLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT          943 a          601 c          387 g         1109 t
ORIGIN      
        1 agatcgcttc tattaattta atttctagct gacttatcat atattcatct acataggtaa
       61 cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata taactatgat
      121 aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat aagataatgg
      181 atagtggaac taataggtac tgccgctttt tggatttatc taccataggt cataccccat
      241 tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc tcatattgag
      301 aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga taatctttct
      361 tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa ttaatatttc
      421 cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat acctgccttc
      481 atttgaatct gccaatggtt cctcattatc ctttatgtta gcatttcgtt ttcgaataga
      541 cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt gtatagatat
      601 gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca ctaatacaac
      661 aatcagagag cccctttttt tcgaaaaaac tagaaaaaaa cataaaaata tttatcattc
      721 tttaaacaga aaatctttct ataaaaaaca aaaaaacgat taggctaggc taatcgtttt
      781 ttgaacattc ttaaaaaaat ccaaattatt ttttataact gaaattgggt attgatgacc
      841 atcgtttctg tagatagtga gcaatcattt taggttttct atctctagta aagattcctt
      901 tcttatttcc ttgcactctt aaaatccctt gactcgttgc aaaatctgca aaattccaca
      961 cttgttcacc aacaaaattt tcacatgtat ctattacttc gtgatttgct tcgtaataag
     1021 ccacttgata ctcttctgta aacattgttg gtactgtatc atgaaatcca gctactgtat
     1081 ctgctccata ttctgtaaac attattggcg tatcaggaca tctttctttc catccatcta
     1141 gctctttttt caacagatat tttgcatgtg ccagatcccc tcccgaaaca taccaaccat
     1201 aataacgatt taaacataaa acatcgacaa tatctcctac cttacaaata tctggcgttg
     1261 attttagata agtaactatt gttacaggtc ttttctgcgg atcaagttct ttagttaatt
     1321 caactaaagg ttcaaaatat tctttagcac cttcggaatc cgaatctggt tcatttgcaa
     1381 tagaccacat tacaacactt gggtggttct tgtcgcgttc tatcaattcc ttgatcaccg
     1441 ttttatgaga attttctgtt cgtatctctt tccacgtatt atgtttttcc acattatcat
     1501 ctaataaagt ggccatgaaa tttaaatgta accctactgc aggtacttca tcaataacta
     1561 caataccttc tcgatctgct aatcgcataa tttcttctga gtaaggataa tgtgatgttc
     1621 taaaagaatt agcacctatc catttcatta aattaaaatc tttaatattt acagcctcat
     1681 tcaaaccacg tccattaaca tacgaatcct catgcttacc aaatccttta aaataaaaag
     1741 gtttattatt aatcaaaaat ttgccattgg taacctgaac actgcgaata ccaaattctt
     1801 ctgtataggt atcgatcact ttattatcat caattaaact aattttcata ctatataaat
     1861 aagcatctag aggttcccaa aaaattggat tcttaagctg aatcattcct ttctttcctt
     1921 cagcttcagc tacaagttga gcctcttcgt catatatcgc tattaagatt tttccggtcc
     1981 attctgtatc gatattgaat tctacacttg catccaaatc agttattctt gtattaatag
     2041 taatatcact tatatagtct ttcggagttg tatataactt aacaggtcta tgaatacctg
     2101 cataattgaa gaaatcaaaa ttaactaagt tttccttttt tatatcccca ttttcctgta
     2161 ctgtttcctt cataatcccc acaggtaaag tactatcgtc aataatattg tttacagcaa
     2221 ctgtaattcg attattcccg tcttttacaa accgattaat ttcagactca aatggtgtaa
     2281 acccaccttt gtgttgagta atcaactttc cattcaaaaa aacttttgct tcatgtgttg
     2341 cagatccaaa acgaagaact aatctttctt ctaataatct tttaggaaga gtcacttctt
     2401 tttcatacca cacccatcca acatgatttc taatgttctc tgtttcaaat aaatcattat
     2461 aagaagaggg aactgaaatc ggataactct ctttttcatt tattggttct aaagcaagtt
     2521 cttcctgtaa gtcatcattt gtatctaatt taaatctcca gagaccgctt aagtcaatta
     2581 cttgcctcgt ctctgtcata atgggataca acatataaac cactcctatc taaatattaa
     2641 ataaatgtat cctttaaacc acaataagca tctattggac ttactccctt aaattcagaa
     2701 agacccatta gtttgtccac caatatttct aaagtttctt gttttccatc ataagtatta
     2761 atataagtct ttacttgagg tacatcagcc aaatgaaacg gacactgtac agatacaaaa
     2821 attgttggaa tttcatgaat gtaaaacggt atatccggag ttcctttact tgctggccag
     2881 acaattcgtt gaactgtgcc tggattaaca ttagctatat taatgatcaa gtcatatttt
     2941 ttagttaaat ttttaattgg tctcttagca gcatatattc cagctatatt tttaattttt
     3001 tcttcttgcg ataatccttt caactcttct tcaggtgaaa
//
LOCUS       DACEOE010000223         3027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEOE010000223 DACEOE010000000
VERSION     DACEOE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.231"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..362)
                     /locus_tag="IW774_002576"
     CDS             complement(<1..362)
                     /locus_tag="IW774_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1154899.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYL"
     gene            complement(546..1763)
                     /locus_tag="IW774_002577"
     CDS             complement(546..1763)
                     /locus_tag="IW774_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1154900.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2146..2229
                     /locus_tag="IW774_002578"
     tRNA            2146..2229
                     /locus_tag="IW774_002578"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2180..2182,aa:Leu,seq:gag)
     gene            complement(2469..>3027)
                     /locus_tag="IW774_002579"
     CDS             complement(2469..>3027)
                     /locus_tag="IW774_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154901.1"
                     /translation="LFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTY
                     VNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNK
                     KTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSV
                     QKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT          963 a          616 c          485 g          963 t
ORIGIN      
        1 agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa
       61 gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt
      121 tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc
      181 gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta
      241 ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat tttttgcgtc
      301 attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat taacgtttcc
      361 ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt
      421 cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc
      481 cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt
      541 taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc
      601 aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat
      661 ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca
      721 tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt
      781 ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt
      841 tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg
      901 gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc
      961 agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact
     1021 cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca
     1081 ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt
     1141 tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat
     1201 tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc
     1261 cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg
     1321 caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag
     1381 tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg
     1441 atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt
     1501 ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag
     1561 tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca
     1621 aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc
     1681 atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg
     1741 ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt
     1801 tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac
     1861 ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc
     1921 atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag
     1981 cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc
     2041 cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca tttttgagct
     2101 tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa
     2161 ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca
     2221 tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt
     2281 tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta
     2341 aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg
     2401 ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt
     2461 tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat
     2521 ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc
     2581 attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc
     2641 catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata
     2701 actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat
     2761 agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt
     2821 ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat cattgccctt
     2881 ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg
     2941 tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat
     3001 gttagaagga aaattaagaa aaatagc
//
LOCUS       DACEOE010000224         2968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEOE010000224 DACEOE010000000
VERSION     DACEOE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.232"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(84..1031)
                     /locus_tag="IW774_002580"
     CDS             complement(84..1031)
                     /locus_tag="IW774_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1154902.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1277..1741
                     /locus_tag="IW774_002581"
     CDS             1277..1741
                     /locus_tag="IW774_002581"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1154903.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1803..2846)
                     /locus_tag="IW774_002582"
     CDS             complement(1803..2846)
                     /locus_tag="IW774_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1154904.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          981 a          587 c          567 g          833 t
ORIGIN      
        1 tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa
       61 atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt
      121 tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccaata
      181 tatttcccga tgattccaag acataacaat tggattccac caatgaacag gaagatcgag
      241 accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac
      301 aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac
      361 ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac
      421 caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaat
      481 ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca
      541 tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg
      601 ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg
      661 ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg
      721 atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac ggtcactaaa
      781 tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa atttctggaa
      841 aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta
      901 ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt
      961 tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt
     1021 gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta cattcaaaat
     1081 ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac aggtattctt
     1141 tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca
     1201 atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctattg
     1261 actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga
     1321 tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca
     1381 gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga
     1441 atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc
     1501 tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg ttgctgttga
     1561 tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat tgccaatcgt
     1621 tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc
     1681 aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta
     1741 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
     1801 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
     1861 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
     1921 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
     1981 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
     2041 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
     2101 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
     2161 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
     2221 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
     2281 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
     2341 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
     2401 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
     2461 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
     2521 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
     2581 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
     2641 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
     2701 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     2761 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     2821 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     2881 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     2941 aaagtcttgt acgacctctt gtttcgaa
//
LOCUS       DACEOE010000225         2967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEOE010000225 DACEOE010000000
VERSION     DACEOE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.233"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            391..600
                     /locus_tag="IW774_002583"
     CDS             391..600
                     /locus_tag="IW774_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154905.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            807..1007
                     /locus_tag="IW774_002584"
                     /pseudo
     CDS             807..1007
                     /locus_tag="IW774_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1673
                     /locus_tag="IW774_002585"
     CDS             1494..1673
                     /locus_tag="IW774_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154906.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1719..2324)
                     /locus_tag="IW774_002586"
     CDS             complement(1719..2324)
                     /locus_tag="IW774_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1154907.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2506..2841
                     /locus_tag="IW774_002587"
                     /pseudo
     CDS             2506..2841
                     /locus_tag="IW774_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          989 a          471 c          527 g          980 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atttaaaaag aaaagtgaca
      361 gataggaata aaaacttatt aaatgtagtt atgatctgtt atatttcatt tctaatacta
      421 ggtcttccac aagcagtgtt tcctgaaatg ttttctggag actggggtat tgtcagctat
      481 tctatattaa cattgattgc tatggtttta ggtacttcat tttataatgc ttttgtaagt
      541 aagcatgttg aggaatatga agtattaatg tacttaaaag agaaaaaaac aagtaattaa
      601 tatgagaaca ccacgcatag tttttatttt gagatattct agaatgaaac acattaagaa
      661 cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc taagtttttt catatttgtt
      721 tgttatgcta tacatagatc tgcataatta gacttgcgct gcccgttatt gaagtttcag
      781 gcagcgctct ttttggggga tcgataatgg aagaagaaaa aatatttgag aaacgctggc
      841 agctagcatc aagtgaacaa agagcaagat acaataattt gatcacctcc taccccactg
      901 ttgactagac atataaggaa aagaaatact tactttggct gtgccagtta gatatagata
      961 cctttgaaac atttgaagtg atcctagata aaattaaaca gaattgatca gaaacaagcg
     1021 aagccttctc tcggttcgct tgttttgcta tttattagtt ggctcagggc aactctaagc
     1081 tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta ccactttaga atacatggat
     1141 gtacgtattg ccattgactc ctattagtta ctgaaaaaaa atttaaacaa gttgagtatt
     1201 gcagataaat agctccagtg aggtgaataa ctgctccact caaggcaata gatagctcca
     1261 tccagggcaa caaactgctc cactttcata tttaatagat gaataacctt ctgtataata
     1321 gaaaggcata cctttcggta tgacaactta tacagaatgt tattttattg gttcactatc
     1381 gaaaaatttt ggagctttat ttttccggta tttctcaacg aacgattagt ttaactgtgg
     1441 gaagctccag acatacgatt cgagaagtta cttctcgagc agacaggaag ggggtgactg
     1501 aactaaccgc tgatatgagc gatacttggc tgcaggaata tctttttccg gaaaaacaaa
     1561 caacagcacg aggttacctt cctgtagact gggaatatgt tcataaagaa ctaatgaaaa
     1621 agaatatgac attgaaactc cttcatagag aatatggggt acatggaaca tagaaacgaa
     1681 aatgcacggt agaaaagcga atgaatatag tataaattct acaaatcatt cgctttcttt
     1741 attttatata tggttggtcg agagagacca ttttctttgg caatttctgc aactggaact
     1801 ccttgattta acatgtccac cacagtttta aagactaatc gtttttgcgg atctgttgag
     1861 ttctcagaat attcaattgg tcgcccctta tatcgaccag cttttttggc caataatact
     1921 ccttgccgct gacgttcacg gattttctca cgttcgtttt gtgcaatata acttagcaat
     1981 gataaaaaca tatcgaacat agcagtatct aggagttcat ttccagtatt aaaatctaaa
     2041 aactgtgcat ctagtatttt taatgctact tttttctgtt tcataaaggt aactgtattt
     2101 tttatgtctt cataatctct gcctaggcga tccagtgatg caacaatgac acaatcacct
     2161 tctcggatat aatttagtaa tttttgaaac tcaggtcgct ggatatcttt accactggct
     2221 ttttcttgga ataatttatc aatgttaagg tcttccatca ttgccaattg tcgatctaaa
     2281 ttttgatctt gtgaagacac tctaatatat ccaatttttg ccataaaaac tccttttaaa
     2341 aaatgatata atttacatgt aaaatttact tgtagtttta ctgttatcat agcattttta
     2401 aaaataaatt ttaagtctac ttataattta ctttggaggt tttgaatgaa tgaaattaag
     2461 cgcaataaaa attcttttgg tgtttattta ggagagccta gctcggaaca gttaaataaa
     2521 ggtgaagctc gtcatgctct atgtcgaaat atattttatg gtagaaaagg caaactttat
     2581 caaacttatt acgacggtat ggaggaacag ctcaacgcat taggattagt aactaatgct
     2641 atcatatatt ggaattcact atatttagaa aaagtctttg aacaaattaa aacggaaggg
     2701 ttcgactgtt ccgaagaagt gttacgcaag caatttccat taattagtga tcacattaat
     2761 tttgttggca aatatagttt ccgctatgaa aaagaattat cgaatggaat actacgtccg
     2821 ttaaatatag agaattattg agaatgttcg tcttttggtt cctaccgtgc attttcgcac
     2881 ggatgttcca gatactattt ttgaatgtat aaaattgaaa acacaagaat actaataaat
     2941 caacgattct tgtgttttta atttttc
//
LOCUS       DACEOE010000226         2918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEOE010000226 DACEOE010000000
VERSION     DACEOE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.234"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(18..1484)
                     /locus_tag="IW774_002588"
     CDS             complement(18..1484)
                     /locus_tag="IW774_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1154908.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(1795..>2918)
                     /gene="cadA"
                     /locus_tag="IW774_002589"
     CDS             complement(1795..>2918)
                     /gene="cadA"
                     /locus_tag="IW774_002589"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1154909.1"
                     /translation="LAVLLAVLPPLVVSGAAFSEWIYRALTFLVISCPCALVISVPLS
                     FFGGIGGASKIGVLVKGSNYLELLAQTETVVFDKTGTLTKGDFSIQTIDTKIDPKIFM
                     QYVASAEQFSTHPIAQSVLEGYAGPLLPTANIQEFAGEGILAEVDGKQVLAGNHKLME
                     RFEISFPSSQEIGTLLYLAIDQSYSGYLVIADTLKEDAVDALVQLKKAGVKNTVMLTG
                     DSKKIADHIGKQVGVDKIYSELLPEDKVQRLEEILQRNNKKTAFVGDGINDAPVLARA
                     DVGIAMGGLGSDAAIEAADVVIMNDQPSKIAEAIHLAKKTLKIVKQNIVFAIGIKILV
                     LALGAFGFASMGDAVFADVGVTVLAVLNAMRSLHVKNKR"
BASE COUNT          895 a          613 c          477 g          933 t
ORIGIN      
        1 taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtgat ttctttgttt
       61 ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac aattggtgtg
      121 atgatattcg tgaataattg gaaccaccat gctttaggat cagcagtata atctactttt
      181 ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc accaaagata
      241 aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt ataagcttta
      301 ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac aactgagttc
      361 aagttcgtca accattttac taattcattc atatctttga taccaaagat cggcaacaaa
      421 ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt acctttacga
      481 ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa caagattttt
      541 agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt tgtcaatgcg
      601 taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg aaaagcactg
      661 tatgccccat ttgccatcaa gtctttagga atattatcgc tagcaaacag catacccatt
      721 gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat catcccttta
      781 ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt ttccgctcct
      841 ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa tgttggaata
      901 tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt gatgaacggt
      961 gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt tccggccagt
     1021 ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa ggcaagaaat
     1081 ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat atttccattt
     1141 ccttggactg cccaaccgcc agcaatcagg attgcttgag gcttttgtgc caaataagga
     1201 atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt agatgtcgta
     1261 ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgaccc taattgtcca
     1321 acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga ggtgacaact
     1381 gatactcctt gctgagcata gttattaact acattcccca tgccccaaac catgttaaat
     1441 gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc tctcctttat
     1501 gataaaaatt tgttatccag atgtaatcat attcaatttt taacaaactt acaagacagt
     1561 gttattttat gtgtgtattc ttttatttaa tacacatcgt tttttcatat tttttccatc
     1621 aaaaaaataa gttattttct tttatttcat ttaccttatt gaatttcaag ctattttgaa
     1681 ttttctgcaa aattaaaaac attagttatt ttataatcct atctattttc tttacatttt
     1741 tcattaagaa aaggatataa aaaaaagagg acgttcattc gtcctcttca ttgatcaacg
     1801 tttatttttt acatgcaagc ttcgcatggc attcaaaaca gctaatactg tcaccccaac
     1861 atctgcaaat actgcatctc ccattgatgc aaaaccaaaa gcacctaagg caagtaccaa
     1921 aatttttatc ccaatagcaa agacaatgtt ctgtttgacg attttcaatg ttttctttgc
     1981 taaatggatt gcttctgcaa tcttcgatgg ttgatcattc atgatgacta catctgctgc
     2041 ttcaattgca gcatcgcttc ccaatccacc cattgcgatt cctacatctg ctcttgctag
     2101 caccggtgca tcgttgattc catctccgac aaatgcggtc tttttattgt ttctttgtaa
     2161 gatttcctct aaccgctgca ctttatcttc tggaagcaat tcgctatata tcttgtccac
     2221 gcctacttgc ttcccgatat gatcagcaat ttttttcgaa tcgcccgtca acatgactgt
     2281 gttcttcaca ccagcctttt ttaattgaac caatgcgtct acggcatctt ctttcaaggt
     2341 atctgcaata acaagataac cactgtaact ttgatcgatc gctagataaa gcaatgtacc
     2401 gatttcttga cttgaaggaa aagagatttc aaatctttcc atcaatttgt gattacctgc
     2461 taatacttgt ttgccatcaa cttcagctaa gatcccttca cctgcaaatt cttggatatt
     2521 agctgttggt aaaagcggac ctgcgtagcc ttctaaaaca gattgagcaa tcggatgtgt
     2581 agaaaactgt tctgcactcg caacatattg catgaatatc tttggatcga tttttgtatc
     2641 gatcgtctgg atcgaaaagt cacctttcgt taatgtgccg gttttatcga ataccactgt
     2701 ctcagtttgt gcaagcaact ccaagtaatt tgaacctttt actaatacac cgattttact
     2761 tgctcctccg ataccgccaa agaaactaag gggtacggag atcactaatg cacatggaca
     2821 tgagatcact aaaaatgtca atgctcgata gatccattca gaaaacgccg ctccagaaac
     2881 aacaagtgga ggtaaaacag ctaataaaac ggccaatc
//
LOCUS       DACEOE010000227         2916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEOE010000227 DACEOE010000000
VERSION     DACEOE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.235"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            12..887
                     /locus_tag="IW774_002590"
     CDS             12..887
                     /locus_tag="IW774_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1154910.1"
                     /translation="MDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYEGNNQLE
                     NRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGRKIVLLP
                     FDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFMINIIAM
                     FALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQLAPKGI
                     RVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLASNKASYV
                     TAQIYGVTGGEAINL"
     gene            925..1488
                     /gene="amaP"
                     /locus_tag="IW774_002591"
     CDS             925..1488
                     /gene="amaP"
                     /locus_tag="IW774_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1154911.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            1502..1690
                     /locus_tag="IW774_002592"
     CDS             1502..1690
                     /locus_tag="IW774_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1154912.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            1703..2605
                     /locus_tag="IW774_002593"
     CDS             1703..2605
                     /locus_tag="IW774_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1154913.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            2637..2879
                     /locus_tag="IW774_002594"
     CDS             2637..2879
                     /locus_tag="IW774_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1154914.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         1044 a          537 c          619 g          716 t
ORIGIN      
        1 aaccaaaatt aatcgacccg cgaaacttgt accatacgga gaaatttcct aaacaagatc
       61 aggatacacc agctttacag cagaatatga ttcctgttcc tgattgtgga gaagaatcct
      121 atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat cactggtgga gattcaggga
      181 tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc agatatcgct cttcaattct
      241 tcccaggaga agaaaaagat gcagaggaag tagcaaaata cgtgaaagtt gcaggacgta
      301 agattgtctt actacctttt gatcttcgtg acgaacaagc acctaaagaa atcatagata
      361 aagccgtgag tgaacttggc gggctagata cgatggtact aaacgcagga cagcaaattt
      421 cttgtcagtc tattgctgag ttaccacttg agcaagtcaa agacacattt atgataaaca
      481 tcatcgctat gtttgcttta gtgaaagaag ctgttccgca tatcccagca ggaggagcaa
      541 tcgtaacaac aacttctgtt caagcattca atccaagcga acatttatta gattacgcag
      601 caacaaaagc ttcaatcgct aactttacga tcggtttagc aaaacagctt gcacctaaag
      661 gaatccgtgt aaatggtgta gcacctggtc caatctggac accattacaa ctagacgaag
      721 gacaaccttc tgattcgatc cctgaatttg gtcagcaaag tttactagaa cgtgcaggtc
      781 agccaggaga attagcacca gtttatgttt tcttagcatc gaataaagca agttatgtta
      841 cagcccaaat ttacggagta actggtggag aagcgatcaa cttataagaa gttgatcgtt
      901 tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa aaacgatcgg agtgatcgga
      961 acattacttt tgctatctgt gcttttgttt gcgatactat taagtgcaac ctatccattg
     1021 ccttttcagc tagagaagtt tcgattcttt tccatcacta attcatacgt gcaacaatat
     1081 gtattttggc ttgccgttgc atttgctatt ttgacaatta tcacgttact tgttttcctg
     1141 ttttatccaa aaacaatcgg aaactttcta ctgaaacatg gtgatgggga gttgacgttg
     1201 gataaaaaag caattgaagg aatggttcgt tctcacttgc atgaagatga attcatccat
     1261 tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg ccgtcaatgt aaaaggggag
     1321 ttgaaacgaa cttcagcact cattggtaaa acaggaacat tgatgaaaga aatcgaaaat
     1381 gaagtacaac acatattggg aacaaaagag ccagtgaaag tatcggtgaa atacacaggc
     1441 tttgaagaac cagctaaacg aagcaatgac cgcaaacgag tagaataggg ggggaccaaa
     1501 aatgaaagaa ctattttcga catacaagct ccctatcatc tttggggtac ttggattgat
     1561 attagccgtt ttatggatca ccgtgggctt tttcaagacc ttattgatct tgatcatggc
     1621 agcgatcggc gtcgctatcg gtttttatct gaaacaaacg aaaatcttag atcactattt
     1681 taaatcttag gaggaaacat ccatgaataa caataacaaa aataataaca ataaaatgaa
     1741 caaccaaaaa ccagtgtctg cacatccaga cccaacgaca ccaaaccaat caaatggtgt
     1801 gaacgatgaa aagaacaaaa cagcaccagt atctgcacat ccagatccag tagtccctaa
     1861 cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa aagcatgcac ctcatcatcc
     1921 aggaactcca gaatctaaag ttccagaaca acatgatgca atcgatcctt ctaaacctgt
     1981 ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa gaaaaactta gttctgacca
     2041 cacaaacgga caaggaaaac ttggttctga tcatacaaat aaccaaggaa agcatgattc
     2101 tgaacaggaa gattcaatgc gcggtgagct gaattatgaa gataaagttg tgcaaaaaat
     2161 catcggaatc gcaattgaac aagtagacgg tttattatct gcaaacggtg gtttcttctc
     2221 taatgtggca ggaaaacttg tgaatacaga taatgtaacg gctgggatcg aaacagaagt
     2281 cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg gaatatggca aagatatcga
     2341 aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa gtaaaaaata tgacacattt
     2401 agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa acaaaagaag aatttgaaaa
     2461 agatcaagaa acggtgcaag acaaagtaac tgacgcagcg aaaaaaaccg gtgaatttgc
     2521 ttcgaatcaa acagataaag taaaatcagc agctggaagc ggcgcacaaa aagtaaaaga
     2581 aaatacagaa ccacgggttc agtaataagg aagtaaaaca ttaggaggaa cgaaacatgg
     2641 gatttatttg gtcacttatc gtcggaggaa ttatcggagc aattgcagga gcaatcacga
     2701 ataaaggttc ttcgatgggg attattgcaa atgtcgtagc aggtttagtt ggatcagcac
     2761 ttggtcaagc attattaggt tcttggggac caagtattgc aggaatggca atcgtgccat
     2821 cattgattgg tgccatcatt ttagtactaa tcgtatcatt ctttgctcga aaactgtaaa
     2881 aagactttta aaaagaggtc gtgaaaaagc tgttct
//
LOCUS       DACEOE010000228         2913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEOE010000228 DACEOE010000000
VERSION     DACEOE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.237"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..995)
                     /locus_tag="IW774_002595"
     CDS             complement(<1..995)
                     /locus_tag="IW774_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1154915.1"
                     /translation="MSTEIQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSQTIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWF"
     gene            complement(1315..1722)
                     /locus_tag="IW774_002596"
     CDS             complement(1315..1722)
                     /locus_tag="IW774_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154916.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     KNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(1741..2559)
                     /locus_tag="IW774_002597"
     CDS             complement(1741..2559)
                     /locus_tag="IW774_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1154917.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          869 a          452 c          404 g         1188 t
ORIGIN      
        1 ataaaccagt tttttacata cacaaaaatc aaattctgca aattatttac tttctcagtc
       61 ttttgttttt gataagcttt cagcaacgtc atatataatc ctttatccgc atcaattccg
      121 taagattcaa gttcttcata cacaactacc gtcttctcaa gttcctcggc tttatgtttt
      181 gcgttgtgta ttgtggtgac ggtttgttgg gcttcttcgt aagtgttaga aaatgttttt
      241 atcaagctta ataccttttc tggtacaaat gtgtttatgc tgtcttcttt catgatttca
      301 acgaagttat ctagcaaaac ttgattttgt aattggtctg tttcagtgtc ttttaagtgt
      361 cttttagtgt ctaaagattt attaatataa taatcttgtg cgatttccgc atttccagtg
      421 ttatcaaggg tttcagctgt tttttctcga cgtgcgattt ccgcatttcc agtattatca
      481 agggttttag cagtttcctc tcgacgtgcg atttccgcat tccgagaaga ggttgattta
      541 tcaatagttt ggcttaaatc gtcatcgaaa gaattctgaa taaaaagatc atcggcactt
      601 atttcaggtt gaagtaaata gtatttattt ttttcatttt ttttcgtttt tgggttaaat
      661 tctcctttta cgactgaaat caaattatat gtttctaact cgtttaaaat ggtttttact
      721 ttgccttggt agcaatttaa tttgtccatt aggtctgctt gtgtaaattc caaatataat
      781 agaccatcat cgtcgatcca tttttcatgt aaggctctac gaaattcatt tttaaaaagc
      841 atataggcta tttttgagct gtctttcatt ttagagtatt tagaattctg aattaaaaaa
      901 ctaggaaatt tataatattt ttcaatataa acattcgttg ctttatatcc ccaattttta
      961 ataatcattt tttgagattg aatttctgta gacattaaat ttctccttct tcatagaaac
     1021 ttgtatatat tcacgatttt caacaagtga actataccat gtattaaggg tgaataaaac
     1081 actttcagta tttaaggtat aagttatata ttaaaaaaat aagtataagt tatactttaa
     1141 atacaaataa tgtataactt atactttaaa taataatttt ataatgttga tttaatcgca
     1201 aatttacacg ttgtttatta agttatactt taaatattta atgtataact tatactttaa
     1261 tattcattta tctagtataa cttatacttt aattgaatac cgcatgaaaa aaaattattt
     1321 tggtaatttt tcaaactctt cttgtatttc ttctcttatg tgttcttctt ttgctactcc
     1381 tagtctatgc tggatatatg agtctgctaa tgtattaaca attttttcga aagaaattct
     1441 ccctttttca ctttgtaact ccgtcatata gtcttgtaaa attgagagtt tgctatttaa
     1501 tgccggagag atctttttag gaactgatac cttgtttctt ttatcaggaa ttgaaactgg
     1561 tcggcctctt tttttcaatg taatattttt tgtgttactc aatgcactat tgctaaagtt
     1621 atattcaccg cttattttca catcttggcg tttgttattg ggtaataatg gctcaaattc
     1681 gccattctcc atcattttag gaatatcaaa atctttaggc attacttatg caactcctta
     1741 ttatttattt aaaaactctt gtctagcatc tagttctgcc aaaatattta taaatacttg
     1801 gtgtgcttta taatcccatt gatcaaaatt tccatttttg tattttctta aatgtatacc
     1861 aattttagaa taccttctca atcttttttg ataatgaatg agatttttgg ataccacggt
     1921 tccgtatttt tcaattgctt cttctaggat ttcttcatca aaatcatcat ttggttctac
     1981 catacaaggt acaatatcta taatttgcaa atcaagatcg tatctatcga ccatgaattt
     2041 ttgataattt acatatttgt tgactccctc taaggattct tcagctgttt gaaatgaaat
     2101 tatcgaatag tctgaagctg ccatagcatt atcggaaaat tcgctaatgg ttggtggtac
     2161 atcaataaat atttcttgat aattgttctt tattggaaga agtaattttt tcaagacagt
     2221 aacttggtct aattcatttt catgtttatt agcaaaagcg gagaatgatc gaaaactagt
     2281 gtcacatgcc agaaggtcaa gattttcatt taccttaata atactacttg ctaattctcc
     2341 atttgcgaca ccatttgtta tagtggtttt gatatcggtt ataccgaaag ttcttgctat
     2401 gacttgtgtt aagttcgctt gagggtctaa atcaataata agcgtttttc tatttcttat
     2461 cattgcattg tcataagcaa gcatgctgac tatcttagtt ttacctactc ctcctttaaa
     2521 attgccaact gtaaatgtag tcccttgaaa agtatccatg taaatcctcc caatctttaa
     2581 ttgataatta aagtataact tatatatcgg aaatggtcaa cattaaaagt ataacttaaa
     2641 taataatatc taaaaacgtt tggatatagc ggtttttaag aataaaagta taagttatac
     2701 cctaaatata tatggtataa cttatacttt aattatttaa ggtataactt ataccttaga
     2761 tattgctttt cgaaaatagc tattctagtc tatctgtttc tttacttttc gctgctgatt
     2821 tgaagtgaat tgatcatatt cattttctag tattaataac ttttcgtata catcggtcat
     2881 attcatacta gaagcaagac ttagatcata tac
//
LOCUS       DACEOE010000229         2876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEOE010000229 DACEOE010000000
VERSION     DACEOE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.238"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1015)
                     /gene="tsaD"
                     /locus_tag="IW774_002598"
     CDS             complement(<1..1015)
                     /gene="tsaD"
                     /locus_tag="IW774_002598"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1154918.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1012..1476)
                     /gene="rimI"
                     /locus_tag="IW774_002599"
     CDS             complement(1012..1476)
                     /gene="rimI"
                     /locus_tag="IW774_002599"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1154919.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(1483..2025)
                     /gene="rimI"
                     /locus_tag="IW774_002600"
     CDS             complement(1483..2025)
                     /gene="rimI"
                     /locus_tag="IW774_002600"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1154920.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2009..2833)
                     /gene="tsaB"
                     /locus_tag="IW774_002601"
     CDS             complement(2009..2833)
                     /gene="tsaB"
                     /locus_tag="IW774_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1154921.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT          796 a          651 c          485 g          944 t
ORIGIN      
        1 attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt
       61 gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag
      121 ggggaatcac tagttctacc tcagggagtt tagttgccaa agcatcctgc aatccttcac
      181 gcaacccttg attggccgct acacctccag ctataatcag ttgcttgact ggataatttt
      241 gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg
      301 ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa
      361 atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg
      421 gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt
      481 aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg
      541 tctccccaat gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc
      601 cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga
      661 tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct
      721 tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga
      781 ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg
      841 tgatttgttc tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat
      901 gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg
      961 ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat
     1021 cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata
     1081 tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc
     1141 attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag
     1201 taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt
     1261 tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc
     1321 ttcagctaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt
     1381 cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa
     1441 gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag
     1501 ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt
     1561 tgcttgaaac cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc
     1621 taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag
     1681 cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc
     1741 taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag
     1801 atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa
     1861 ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa
     1921 ttctcttagt acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt
     1981 atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag
     2041 gtgtgtgcgt ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa
     2101 aagcttgttt ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac
     2161 ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt
     2221 cagagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta
     2281 gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat
     2341 tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca
     2401 caataatatt atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta
     2461 ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc
     2521 ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt
     2581 gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga
     2641 tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt
     2701 ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct atgacattct
     2761 cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag
     2821 tacaataata catgattatc tgctggcggg caaattttgc cccttcctga aacaca
//
LOCUS       DACEOE010000230         2829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEOE010000230 DACEOE010000000
VERSION     DACEOE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.239"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..304
                     /locus_tag="IW774_002602"
     CDS             107..304
                     /locus_tag="IW774_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154922.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            308..919
                     /gene="mobC"
                     /locus_tag="IW774_002603"
     CDS             308..919
                     /gene="mobC"
                     /locus_tag="IW774_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1154923.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            904..2295
                     /locus_tag="IW774_002604"
     CDS             904..2295
                     /locus_tag="IW774_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1154924.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            2511..2825
                     /locus_tag="IW774_002605"
     CDS             2511..2825
                     /locus_tag="IW774_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154925.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
BASE COUNT         1108 a          399 c          525 g          797 t
ORIGIN      
        1 caagacgaag attcacttat gaagacaatg agttattcag agatacactt aatgatccta
       61 atccactttt cgataattta ttaagaaata aaaggggggg attgtcatgc aattaattct
      121 aaagattagt acgcatgatt tcttagaact tgacgaatta tgtgaatcag taaagaacgc
      181 aacaaatgaa aaaatcacgg tcactaatta tattgaactg atgattcaag aagaattaaa
      241 gatgaaaaat gaaatagaga gcaatttcaa atgttgctct ctagaaaaaa atgtgagggg
      301 ataatcaatg gatgataaac aaaaaatgta cgaaaaaaat cgcaaacata gtgaggcctt
      361 aaattttcgt gtcgataaaa agacttatct tgaactgaca ttgattgcaa aacaaagggg
      421 aatttctaga gcagacgttt gccgtgagcg gttaaaggga ctcaaatatg aaccgccgtt
      481 gttagctgaa gatgataggg tagttttaac caaacagtta aatcaaattg gtaacaattt
      541 gaatcaagcg gtaaaggcat taaatatcat tgctaaatat tttagagaag aatctgaaga
      601 taaaaaagaa aagagcctac attttagttt tgatgctagc cgttacaaca tttttaaaga
      661 tgaacttgcg aatagcatag caaaaaagtt gatgattagt tatccagatg acattttttt
      721 ggagtccaat cggagtgccc cctctttttt taaattatca gaagtagaaa aagaatattt
      781 cgaatattgg atagaaaagc aaactagacc gggtacgaaa gaagagtatt cccaaaaagt
      841 tgatcggaat ttaattcact caattcagat tattgcaagt ttaaaagatg aggtgaaaca
      901 actttgggag ctactataac atcgaaagga cgcaagaaaa atttatcctc gctattacaa
      961 tatgcagcta atccttcaaa aaaagaaaaa tgtgttgcag tctcaggaat aaattgtagt
     1021 gatgatagtg aggaagcact gcaagaaatg aatttagtac gtaccaagat gaataaatct
     1081 aaagatatag cacaagcgtt tcatgttatt cattcgtttg ataagagtac ttctaaagag
     1141 ttaagtattg ataaaatgca cgaaatagct gtcgaatttg cagagaaagc ttttccaaat
     1201 tcgcaaatca ttgttgcaag tcacaatgat aaagatcatt ttcatagtca tcttgtcatt
     1261 aataatatta acatggaaac atgtaaaagg ttgcgtattg accctaaaga tttagaacta
     1321 atgcgaaaaa ttaacgatca agtcctcaat gaacatggtc ttgaatcagt gaataatgag
     1381 tactatgaaa acttagaaaa gagaaccaat ctttattcaa acgatccttc taagatgaaa
     1441 tatggaaaaa ttaattatca agagtttgtg caaaatgcga tcgaagcaac gttggaaaaa
     1501 tgcgaagtga attcttttga agatttttcg gatcatttgg caaaagaata taacattgaa
     1561 gtttatcaat tttcaaaaac aaataacaaa cttggctatg ttctttacaa acaaggtgta
     1621 aatttctatg agcgtagagg tgagttaatt actttaggaa gagataaaca atctagaaaa
     1681 gaaaaaagtc agtatgtcga acgtactttt agtgcaagaa agttagggaa aagatattca
     1741 tttgaatctg tttctgaaca acttgaaaag aatttagaag aatcacatat aaaaaatttt
     1801 tttaatatgg aaagagagca agttatgtcg ctaattagtg gagtaaatta caaaactggt
     1861 gagttaaatg taccgtttgt ggaattagat tccatcgcac aaaagcaatt tagcgaccgt
     1921 gaaggcgatg aaaaattcat taaatatgat gacttcttgc gagaggtaga caagcatttt
     1981 gtaaatacaa tgtctttaga agatgatcaa gaatacacga ttaaacttca tacggatgtg
     2041 aatcctcaaa ctttacgtag tgcgaaaagt aaaacaattt ctattaaaaa gactgatcag
     2101 aaagagttat ttctaccggc aaagggtttt gaaaattgtt ctcatacacc ttctgctaaa
     2161 cagattcact atcaaatcag tcgcataatg aatgacgatg taaaaatgta tcgtgctaaa
     2221 aaggattatg aattcacaca acaaatgatt gaaaatcagc aaaaaaggca acaaaatttt
     2281 gaaatggaat tctgaatggg cttgttagtg gactttccag ttttttgcct atgaagagtt
     2341 gcttggaaag aagcagtttt taggacatac gccagctacc catctacttg ccagtcaatc
     2401 cgccccgctc acgctaggct actttctggc aagtcagatg gcgggtaaat ctctgaggga
     2461 gcaagctccc tacagactcc ctcccaaaaa aagaaaggtg gaagaaaaaa atgaactatc
     2521 catattttaa agtctcggct tcagaagaaa ctaaagaaat attcaacaat ttttacaacc
     2581 aaaacaaagg aatttttggt tcaaaggcaa atatgtttcg tgttatggtt tcaaatttac
     2641 cagtgttagc tagtccatca aataataaat ttaatgatcc tgaaagcatt aaatttgaac
     2701 aaaaaatttc agaactagaa tcaatgataa gtaatgaagt aattgaaaag ctggatgaca
     2761 ttgatcaaaa gttatcgtat tctctaaaaa acaaatacaa aacggaggag aaaaaagatg
     2821 tttaaagtt
//
LOCUS       DACEOE010000231         2790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEOE010000231 DACEOE010000000
VERSION     DACEOE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.240"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..108)
                     /locus_tag="IW774_002606"
     CDS             complement(<1..108)
                     /locus_tag="IW774_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1154926.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAG"
     gene            complement(135..443)
                     /gene="rplU"
                     /locus_tag="IW774_002607"
     CDS             complement(135..443)
                     /gene="rplU"
                     /locus_tag="IW774_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1154927.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            725..1366
                     /locus_tag="IW774_002608"
     CDS             725..1366
                     /locus_tag="IW774_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1154928.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(1428..>2790)
                     /locus_tag="IW774_002609"
     CDS             complement(1428..>2790)
                     /locus_tag="IW774_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1154929.1"
                     /translation="TKGMQGVLLATFLAGLFLLAAGLFRLGTLTSFIPAPVITGFTSG
                     IAIIIALGQIDNLFGVHSEGTNVIEKLSSYQTIGFPINTASLIMGGLVILGMFIYPKK
                     WAKRMPSSLLAIILATAVMLLFHLPVATVGKIPQTLISSNRLNMGDFSFSALQQVAVP
                     AISIALLGMIESLLCGASAGRMANKPLDSNQELMAQGIGNLLLPFFGGIPATAAIART
                     SVAIKSGAQTRLAGMIHAVVLLLSMLVFAPVMSQIPMPALAGVLIVTAWRMNEWETIK
                     ELFTNKYWSAVLLFILTMICTVIFDLSIAIVIGVIGGCVFFVVKSAAITISLETIDWQ
                     RMGLPETEKLNNWTVVYISGPLFFMSSEKLKNKLEELNHHDGVIFSMRGVPSIDLTAR
                     AVFDEFQEKAEQRGQTIIYTSLQPEVEKQLSHLWEHTFTHQHQTVAHALTALHAKYTA
                     ISH"
BASE COUNT          859 a          617 c          504 g          810 t
ORIGIN      
        1 acccgcacaa acgatatcac ttccataagg accagcatca gcatgcccgg tcaaagtgaa
       61 tgaaacgatc tgacctgcgt catttcgctt aaatgttcct ttgatcatat ataggccttc
      121 tttctcaaac gctattaagc gttgatcgcg tcgataacca cttttgtata tggttgacga
      181 tgaccttgtt tacggtgtga atgtttttta ggtttgtatt tgaaagtaac gactttcttt
      241 tgtttgccgt gtttttcaac agtaccttca acagttgcac ctgcaacagt tggagcgcct
      301 acttttgtag attctccgcc tactaagata acttcgtcaa aaacaacttt ttctcctgct
      361 tcaacgtcaa gtttttcaac atagattgct tgtcccactt caacttttac ttgtttacca
      421 ccagttttaa taattgcgta catgctataa agcacctcct tctgatatac ttagactcgc
      481 catccgaagt gacagcatat gctgtgctta ttacttgttc tgagcggttg tagctgtgga
      541 gtccacaatt acaacagtac tagcttacca gaacagcaac cgttagtcaa ctaaatttaa
      601 aatcaaaaag taaaacaaaa attcaagcaa tacttctgct tttctatcct cacagttcta
      661 ccaaataaaa tagtcgcatt gtgtttcttt tattcttata ataaaagaaa aaggaggagc
      721 ataaatggaa tacccaaaag aactgataca agaagcttct ttaagaatga acggccgtcc
      781 tgtagaagga tttatcgctg ggcaaggacc tcttcatcct aaactgatgc tcgtgggaga
      841 agccccaggg aaaacagaga ttgatacgca tgttcctttt agcggtcaag ctggaaaaga
      901 attgatgcag gcactttctt caactggctt aacacgagaa gaggtgtata tcaccagtgc
      961 tgtaaggagc cgcccgtatc gcgtgactca tcggatcaat aaacggacac aacagtcaga
     1021 aactgtttat ccaaatcgga cacctactcg ttccgaagta tttgcgcatg caccaatatt
     1081 ggattacgaa ctgatgaagg tccagccaaa attaattgcg acacttggaa acattggtct
     1141 tcaacgttta cttggcaaag aatatacggt taccaaaaat catggtcaac tctatacagg
     1201 gcctgtctta caattaacag aacaaaagga tgcctacgaa tggtcagaaa aaaactatcg
     1261 aattcttcca ctttttcatc cagcagctat tttctacaat cgctcccttg tacctgttat
     1321 ccatcaagac tggcaagtac tagcttcact tttacagcaa tattaaaaag aacagacaag
     1381 accataaaat cttcagttta cagtcttgtc tgttcttttt cattgtttta atggctaatt
     1441 gcagtatatt tcgcatgtaa agctgtcaac gcatgagcaa ctgtttgatg ttgatgcgtg
     1501 aatgtatgtt cccacaagtg agaaagttgt ttttctactt ctggctgcaa ggaagtatag
     1561 ataatcgttt gtcctctttg ctctgctttt tcttgaaatt catcaaaaac tgcacgtgcc
     1621 gtcaaatcaa tacttggtac gcctcgcatg gagaaaatga caccatcgtg atgatttagt
     1681 tcttccaatt tatttttgag tttttctgaa gacataaaaa acagtggacc actaatatac
     1741 actactgtcc agttattcag cttttccgtt tctggcagcc ccattctttg ccaatcgatc
     1801 gtttctaatg aaatcgtgat agcagcgctt ttgacaacaa aaaatacaca gccgccgatc
     1861 accccgatca cgatagcaat acttaaatca aagataactg tacaaatcat cgtaaggata
     1921 aacaatagaa ctgctgacca atatttatta gtaaatagct ccttgatcgt ttcccattca
     1981 ttcatacgcc aagccgtcac aatcaaaaca cctgcaagtg ctggcattgg aatctgtgac
     2041 atcactggcg caaaaacaag catggagagc agtaagacaa ctgcatgtat cataccagct
     2101 aatctagttt gtgcaccaga tttgatggcc acgcttgtac gggcgatggc cgcagtagca
     2161 ggaatcccgc caaagaaagg aagcaacaga tttccaattc cttgcgccat taattcttga
     2221 ttgctatcta agggcttatt tgccattcgg ccagccgaag caccacatag caagctttca
     2281 atcattccta ataaagcaat gctaatagct ggaactgcca cttgttgcaa ggcagaaaag
     2341 ctaaaatctc ccatattcaa acgattacta ctgatcaatg tttgtggaat cttacccaca
     2401 gttgctactg gtaaatggaa caatagcata actgctgttg ccaaaatgat agccagcagc
     2461 gaactaggca ttcgtttcgc ccattttttt ggatagataa acatccctaa aatgactagc
     2521 cctcccatga tcaaagaggc tgtgtttatc ggaaatccta tcgtttgata actacttagt
     2581 ttttcaataa cgtttgtacc ttccgagtga acaccaaata aattatcgat ctgacctaat
     2641 gcaataatga tagcaatacc tgaagtaaac cccgtaatta cgggtgctgg gataaaagaa
     2701 gtcagtgttc ctaatcggaa aagtcctgct gcaagtaaaa atagacccgc taaaaaagta
     2761 gcaagcaata caccttgcat tccctttgtt
//
LOCUS       DACEOE010000232         2744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEOE010000232 DACEOE010000000
VERSION     DACEOE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.241"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(9..371)
                     /locus_tag="IW774_002610"
     CDS             complement(9..371)
                     /locus_tag="IW774_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154930.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(445..825)
                     /locus_tag="IW774_002611"
     CDS             complement(445..825)
                     /locus_tag="IW774_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1154931.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            907..2082
                     /locus_tag="IW774_002612"
     CDS             907..2082
                     /locus_tag="IW774_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1154932.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2159..2743)
                     /locus_tag="IW774_002613"
     CDS             complement(2159..2743)
                     /locus_tag="IW774_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1154933.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          840 a          539 c          470 g          895 t
ORIGIN      
        1 tctgtatttt agaagaaagg cactccctct acttcgtaag ttccaagaac agagcgcatt
       61 tgacctggtt cttcccaaat atatccttcg cctaattctt ctctttgatt cttttgttca
      121 gtttcaaaga caaacaacgg aacattttcc tctttaatat gtgtatttgt caattccatc
      181 aaactaatct tgctaacatc gatatgaact atttctttta agaaattaag tatattcgct
      241 aaaccagtct ttccttcaga aagttccgct aacgcaaatt ccaacttgtc atcaattgaa
      301 tggaccaaaa attttttagt accatcctcc aaatgcaaca taattgtacc agcaactctt
      361 ttgcttgcca aaatttccac ctcgatcaac ttgttctatg gcttacataa aaattctagc
      421 acattttcag aaaaaataca ctttttattt cttattttct ttccgttcta gttcagataa
      481 tgcttcttct atccaaatag gtaactgctc cgcaatagtg gtaaatccga tcttacgatt
      541 tttattcgtg aaataccttt ttctgcgata tggaacagct ggatttgttt tttctaacgt
      601 tcttaaagat aaactgattt ttttggaata ttcatcaatg tcgatgatct gaactgttac
      661 tggctgtccg acttttaaca atgaatggat atttttcgta taaccggatt gtacttctga
      721 aacatgaata agtccttgcg tctcgttatc aagagaaacg aatgcgccat aaggctgtat
      781 ccctgtcact tttccttcaa ttatttgtcc aattttataa gtcatctact tccctctctt
      841 cgcatttatc attatactgt aaatagctca aaaaaagaag cgacgaaaat gactggaggt
      901 cttaagatga tcgaatttga taaacagatt tcaagaaaag atacagaaag tgtaaaatgg
      961 gacgcaattg ctgagacata tcaaatggat gacttacttc ctttgtgggt agcagatatg
     1021 gacttcttgt ctcccggtgg ggtagctaaa gcgtttagtg aatacatcaa acatggaatc
     1081 tttggttatg ccactctgtc tgataaacta tatcaggcta tcattcattg ggaacgccaa
     1141 caccatgctg tggagttgac gaaagaaaat atcgttttta caagtggtgt gttaaccagt
     1201 ttagctgcag cagtacaaac ttttacaaaa ccaggcgatt ctattttgat tcatgaccca
     1261 gtttatcctc cattttcctc tattatagaa acaaatcaac gtcacatcgt tcgaagctct
     1321 ttgctagaaa aaaataatca tttcatcatg gacttgtctg acatggagaa aaaaatcgaa
     1381 gaaaacaatg taaaagcaat gattttatgc aatcctcaca atcctggagg gcgtgtttgg
     1441 agcaaagaag aattgaaaca gttaagcgaa ttgtgtttaa aacatcaagt gattctgttt
     1501 agtgacgaga tccatcaaga tttaacttta ttcgatcata catttacttc catgctaacg
     1561 attgatccta atttagatga tctactagtt gcttttactt ccgcaaccaa gactttcaat
     1621 ctagctgcaa tcaaaaattc aatggtattt atcaaaaatc cagagatgaa agctgatttt
     1681 gaaaaacatt tagtgatgaa ccaacagcac gaaatcaaca cttttggact gattggaacc
     1741 caagcggctt atgagacggg tggagaatgg ttaagccaac tgattcctta tctagaaaaa
     1801 aatgttcaaa cagttaatca gttttttgaa cagtatcttc cgaaagtgag aataatgaac
     1861 cctgaaggaa cttacttaat gtggttagat tttaccgcct atatttcaga cgaccaagaa
     1921 ttggagaaca ctcttgttcg gaaaggaaaa gtcgtattaa atcctggcat cacgtttggt
     1981 ccttccggtc atgggcatat gcgcttgaat attgcttgtc cagaagaaac tttactggaa
     2041 ggacttaatc gaatcaagta tgctttagag acaatcgatt aaatcattta ttcaaaataa
     2101 aaggacgaga ccttactagg tttcgtcctt tttcataatt actccgtacc ataccaaact
     2161 attctgaaat ttcaattgtt tcaatcacta catcatgtac aggtcggtct tgtggaccac
     2221 gttgtacatt tgcgatttca tctactgtgt ccatcccttc tagtacatgt ccaaagactg
     2281 tatgtcggaa atctagccaa ggagttccgc cacctttata cgcttcaatg atttctgatg
     2341 ggaatccagc accttctagt tgtccaagca tattagctgg cacatttgtg ttatttacaa
     2401 taaagaattg gcttccgttt gtgtttggac ctgaatttgc catagataaa gctccgcgaa
     2461 ggttgaacag ttcacgactg aattcatctt caaaactttc gccataaata ctttcgccac
     2521 ccattcctgt tccagttgga tcgccgcctt ggatcatgaa atcaggaata acgcggtgaa
     2581 aaatcacgcc atcataataa ccttttttgg aaagttcaat gaaattttga accgtcttcg
     2641 gtgctagttc agggaataat tgaacagtaa tgtcaccacg gtttgttttg ataacagctt
     2701 ttggtccttt ttcacttttt aaatctagtt gtgggaatgc cata
//
LOCUS       DACEOE010000233         2732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEOE010000233 DACEOE010000000
VERSION     DACEOE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.242"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..794)
                     /locus_tag="IW774_002614"
     CDS             complement(<1..794)
                     /locus_tag="IW774_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1154934.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLIIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQFKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYV"
     gene            complement(914..1561)
                     /gene="eda"
                     /locus_tag="IW774_002615"
     CDS             complement(914..1561)
                     /gene="eda"
                     /locus_tag="IW774_002615"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1154935.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1654..>2732)
                     /gene="uxaC"
                     /locus_tag="IW774_002616"
     CDS             complement(1654..>2732)
                     /gene="uxaC"
                     /locus_tag="IW774_002616"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1154936.1"
                     /translation="LTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDDP
                     TDDLGYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAAIPIKDFNGLK
                     QALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQFV
                     TGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVAL
                     FDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMRQ
                     QLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYLS
                     QIVENICYRNAYNYFNFFEIGSRK"
BASE COUNT          877 a          567 c          372 g          916 t
ORIGIN      
        1 aatacataat tattcatact cataattaat aattgagcaa ggagtaagaa aatagccgca
       61 ccaattatag aaagtaatgc cctattttta actaaccctt ttaaagattt taacaatgaa
      121 actttgaatt gagattggtc aattcttact cgttcagtag ttagtttata acatattaaa
      181 taacaaataa gtgcacatat cgaaaatatt cccgcaacaa ttgtaaaagt tgatccacca
      241 cgaactattt gagcgcctga atcatctctt gtataaataa ataatggtgt taatacacca
      301 atgatcaaac ttgctaaagt agctccaaca tttcggaatg tcgatagcat tgttctttct
      361 tctgtaatag aagtaatagc tgatgccatt gatccataag gaatatttat agatgtgtaa
      421 aaaatactcc cccataatat atacgtaaca tacatgtata ctaccttaac gctcatagaa
      481 gctgaaatca tagaagtttg atacattaaa aaacttgcta aagcgactgg accactcatt
      541 cttaaaatcc acggtttaaa cttaccctgc ttagttgctt tcgatctatc aacaatacgc
      601 cccatagtta catcagtaac tgcatcgata aatcttgcta ctaaaaataa tgttccaacg
      661 gcagctccgc ttatccccaa tacttttgta taaaagatca ttaaaaaaga actagcaaaa
      721 atgaaagtaa aatcgtttcc aaaatctcca aacatatatc ctattttgtc tcttatacca
      781 aattttcttt ccatacaatt ctctcccttc ttttgttaag cgttttcatt cttatgaaag
      841 tacgtactat actctgaagc gtaaagagtc agtatttatt ttttacattt cagagtatta
      901 tttgtttttt ctcctaataa ttctttatct cataaaactt atctatatat ttactagcta
      961 ctcgacttac ttcttcaaaa tcaccttttt caacagaagc caataaattc ccacctacac
     1021 caactgcaac aactcctact tcaaaccaat cttctaagtt ctctagactt actccgccag
     1081 taggcattat atttacgtgt ggtaatggag cttgataggc ttttacaatg gatggaccaa
     1141 aagcagaacc tgggaatagt ttaatgatat ctacgccagc agtaagtgcc tcctttatct
     1201 cagtaattgt catacaacct ggtaaataag gaatttgata taaattacaa atctcagctg
     1261 tttgtcgatc aaaagatgga ctaacgatat attctgcccc agccataatt gcgatacgag
     1321 cagtaaacgc atctaaaacc gtaccggctc cgattattac atctttttga tcagcatatt
     1381 ttttagcaat ttctttgatt atttcttcag cattaggaac tgtaaaagtt agttcaatcc
     1441 ctttaattcc acccgcaata attgaattta ttgctttaat ggcctcatcc ttatttttcc
     1501 cacgtacaac cgcaatcact ccagccgctt ctaacttact taaaatttct acttttttca
     1561 tatctatctc ctctatctcc ttcttttatg gatacatact ttgtctagct gtactaactt
     1621 aaaaagaata aatttaagct tttatttcaa gtattatttt ctacttccga tttcaaaaaa
     1681 attaaaataa ttatacgcat tacgataaca aatattctcc actatctgac taagatacat
     1741 ttcatcatct ggaattcggc ctgctttaac ccactcgctt agtaattgac ataatactcg
     1801 acgaaaatac tcatggcgag gatagcttag aaaacttcga gaatcagtca acatacctgt
     1861 aaaattcgac aacaaacttt gttgcgccat cgtagtcaac tgttgacgca ttccttcagc
     1921 tgtatcattg aaccaccaag cacatcccaa ttggattttt tgaatcatac ccccttggaa
     1981 gctttgcatt gctgttgcta gtggaagcca atcagttgga tttaatgaat ataaaattgt
     2041 ttttggtaag cacccactct tttcagcggt atcgaacaat gctacaacat tttgtgtcaa
     2101 actagcttga tcccccatcg aatcaccacc gacatctact ccaatttgtt cctttaatat
     2161 acttgaatta tttcttaaag catttaaatg catttgcatc gtccaaccat atcttgtata
     2221 ctcagacatc aaatgtaatt gaatacctgt aacaaattgt gcaatttctg ataaatggaa
     2281 atccgttttt cctgctaaag cttttattaa cagattgtcg agtatttttt catctgctat
     2341 ttcataaaaa taagtattca aaccgtgatc tgctaatcga cagccaattt gatgaaaatg
     2401 atccactcgt tggctcaatg cttgcttcaa cccattaaag tctttgattg gtatagccgc
     2461 tgcttgacta agttttaaaa tatagttcga aaagcctgat gatttgatac tccagacctt
     2521 atctggacgg aaagtaggta ataccttgaa agaattatcc tgtttagaaa gctcagtatg
     2581 ataccccaag tcatctgtag gatcatccgt tgtacagatg gctttcacat ccatcatctc
     2641 caacaattct ttcgggcgat aggaatccgt ttggattttt tcgttagcac gtcgccaaat
     2701 ttctggtgca ttcttctcag aaattgtcaa at
//
LOCUS       DACEOE010000234         2732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEOE010000234 DACEOE010000000
VERSION     DACEOE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.243"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..553)
                     /locus_tag="IW774_002617"
     CDS             complement(<1..553)
                     /locus_tag="IW774_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1154937.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGV"
     gene            complement(740..1390)
                     /locus_tag="IW774_002618"
     CDS             complement(740..1390)
                     /locus_tag="IW774_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154938.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1933..>2732)
                     /gene="whiA"
                     /locus_tag="IW774_002619"
     CDS             complement(1933..>2732)
                     /gene="whiA"
                     /locus_tag="IW774_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1154939.1"
                     /translation="QTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLK
                     QGTKDVLMDLDIMDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLE
                     IFSIYEQHNQDICDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRF
                     EDVRIVRDMRNSVNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIA
                     ELRLQHPEVSLKELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
BASE COUNT          901 a          553 c          375 g          903 t
ORIGIN      
        1 tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac aaccttgatc
       61 cttcttttat agattcagga aacagtataa ttttagtact agataaattg tttacaccga
      121 ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac aaaagaacag
      181 ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta taaatactct
      241 ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct atccaagcaa
      301 cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt aatctattta
      361 tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa ttaaaaatcg
      421 gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa taacctgatt
      481 ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat atccataatc
      541 tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag catcaccaat
      601 tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc atgcttcaac
      661 aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac ataaatttta
      721 cattctgttt tttataagac taataataat cattgaaaaa atttcttatc ggtggcgcat
      781 attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct tcttctaagt
      841 catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc tgtggcttgc
      901 ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt gttacatctt
      961 cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta ttatttaaaa
     1021 gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa cttcctataa
     1081 attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca tattcaagac
     1141 taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt ctccaaataa
     1201 atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg agacctgctc
     1261 gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct agaaaaataa
     1321 aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt tgccctccct
     1381 ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt caggtataac
     1441 gtataccaac catacatccc aattactatt tatgaactta tgattatcac tgaataaatc
     1501 actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc gactagctaa
     1561 tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac cagaagtaag
     1621 aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa tctccagtta
     1681 tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa gtttatgtat
     1741 aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat taaaaaacaa
     1801 tagaagcctt tcaacattat gttactccag taaagttgga attatagttt gacggaagaa
     1861 aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc atgtatcagt
     1921 tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat ttattttgcg
     1981 aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt cccctaattc
     2041 tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa gtttttcagg
     2101 aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt gtttagaagc
     2161 cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat tgaccgaatt
     2221 tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg ttgcaccaat
     2281 caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat agccattgcg
     2341 ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa tgtcttggtt
     2401 atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg gattattgac
     2461 tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg aatttcccat
     2521 aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa gatccatcaa
     2581 cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt ttaatttcat
     2641 ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa gagaatagat
     2701 acgtctggca attgcagcat tttctgtttg ga
//
LOCUS       DACEOE010000235         2719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.244, whole genome shotgun sequence.
ACCESSION   DACEOE010000235 DACEOE010000000
VERSION     DACEOE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.244"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            81..239
                     /locus_tag="IW774_002620"
     CDS             81..239
                     /locus_tag="IW774_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1154940.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            311..1537
                     /locus_tag="IW774_002621"
     CDS             311..1537
                     /locus_tag="IW774_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1154941.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(1626..2018)
                     /gene="rpsI"
                     /locus_tag="IW774_002622"
     CDS             complement(1626..2018)
                     /gene="rpsI"
                     /locus_tag="IW774_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1154942.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2034..2477)
                     /gene="rplM"
                     /locus_tag="IW774_002623"
     CDS             complement(2034..2477)
                     /gene="rplM"
                     /locus_tag="IW774_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1154943.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT          934 a          469 c          474 g          842 t
ORIGIN      
        1 aaattcctat gtgcttaccc aaagcaattc aaaaattcta atctttttta ggagatgatt
       61 ttataaaatt gagagtctac ttgaataaaa aacaaacctg tgagtttcta ggaattgata
      121 ataatacttt agataagtgg attcgtatat ataattttcc agtaatcaga attgacggta
      181 tagttcgctt tgacatagag gatatcaaag attttatgaa tacatttaaa acaatttgaa
      241 aatttcttct cgccgcatat actaagaata gcgaaatata ctgccttact ttttaaggga
      301 gtgaggaatt ttgcctacaa aactttcaaa tgggaaatat aaaactaatc tgaggtatcc
      361 aaaaaaattc agagaaatca ctggaattac atcagaaaag tatcaaaaag tatttccaac
      421 taggcaattg gctattaaag ccgaaaatgc aatgaaaagg aaaattgaaa cagttttaag
      481 agaggagaat gcaaattcac tagaattaaa aggaaaaata aaatttaagg aattctatga
      541 atcaaaatgg cttcctagat atgaactagg tcagactact agatcaaaaa gagctccaag
      601 ttacgtaaca attagtaata ctaaagatat attccgattg cacatccttc caatgtttgg
      661 tgagtatgca atgaactatc taaattcaaa tactgagata atatcagatg aattaacaaa
      721 aaaatcgaaa gaatacgcaa atataaaaat tatcaaagga tatgtccgtt ctatgtttga
      781 tattgcagaa atactaaatt atattgaatt taaccgaact accaaaatta ttcaatgtat
      841 cactgcacct aaaaaaattg ctttagaaga aagaaggata cgggaaggta atcaagcatt
      901 atcgtcagaa gagcttattg cttggataga agcagtaaac actgacttga ataatcactt
      961 gcttactctt catgactata ctcttttcat gcttacccta tatcttggtg atagaaaaag
     1021 tgagacgtat gctttgcaat ggaaatatat cgactttgag aaacaaacgg ttcgccttaa
     1081 acatgctttg gataaatatc aaaaaaagaa atttacaaaa ggtcgaaagg ataccgtaat
     1141 tcaagtaccc gaaattgtaa tggcattact aagcaaatgg aaatcagtgc aagctgagca
     1201 attgttgaga ttaaaaatta atcaaacacc agatcagtat ctctttacct atactaaacc
     1261 atccggagaa gtaaattgcc ctgttcacgc tgattaccta aactatcgaa tcaattcaat
     1321 taaaagacga catcctgaat tggctcattt atctccacat aagcttcgtc atacctatgc
     1381 aactatagct agacaaggcg gagcaaatat gaatcaaata tctagtgctt taacacactc
     1441 agatatttct acaacgagaa tatacgtaaa tacaccagat gtagtagata aaactgtttt
     1501 tgaagccttt cagagaggat tgaataaatg tgattagtat gtgttcttaa atgtgttctt
     1561 aaacaagaaa aagacacttt tcaaacaatt gaaaagtgcc ttgaaacatt gatatataaa
     1621 taatattaac gtttagagaa ttgagatgct ttacgagcct ttttgagacc tggtttttta
     1681 cgttcgacca tacgtgcgtc acgagtaagt aagccagcac gtttaagagc taaacggaag
     1741 tcaggatcaa cttctagcaa tgcacgagcg ataccatgac ggatagctcc tgattgtcca
     1801 gcgtatcctc cgccgtttac gttaacaaat acgtcatatg cgccttttgt ttcagtaaca
     1861 gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg gaatgtattc ttcaacatct
     1921 tttttattta cagtaatttt gccagttcct ggtactagac gtacgcgagc aactgcgttt
     1981 ttacgacggc ctgtgccgat atattgaact tgtgccaatg aaattccctc ctattagatt
     2041 aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat gtggatgttc tgctccaccg
     2101 tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt ttttaggaag catacctttt
     2161 acagaagttt cgatcaaacg acgagaattt ttagcacgca attcgccagc agtgattgat
     2221 ttcaatcctc ctgggtagtt gctgtgacgg taataaattt tgtcagtcgc ttttttacct
     2281 gttaatttca ctttgtctgc attgatgacg atgacgaaat caccagtatc caaatgaggt
     2341 gtgaaagttg gtttattttt accacgtagt acagatgcaa caactgttga aagacgtccc
     2401 aatggaacat cagttgcgtc tactacatac catttacgtt ctacttcgcc tggtttggcc
     2461 atatatgttg tacgcacgtt attttcctcc aatttaagtt ctgatgtttg acatacactt
     2521 cgagtttccg gggctctttg tggggcaaac aataccattt aacatattac ccgctaatct
     2581 agtcaatgtc aacgtttttc tgcttgattg aaaaatttat atgaaacttt acttcttttc
     2641 tttcttttat ataaaagggt cgtttcctgt caaaatgaaa aacaaaaaga ggctgtgaca
     2701 aaagtcttgt cacaacccc
//
LOCUS       DACEOE010000236         2717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEOE010000236 DACEOE010000000
VERSION     DACEOE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.245"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..670)
                     /locus_tag="IW774_002624"
     CDS             complement(<1..670)
                     /locus_tag="IW774_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1154944.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMV"
     gene            complement(814..1545)
                     /locus_tag="IW774_002625"
     CDS             complement(814..1545)
                     /locus_tag="IW774_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1154945.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(1614..2555)
                     /locus_tag="IW774_002626"
     CDS             complement(1614..2555)
                     /locus_tag="IW774_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1154946.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
BASE COUNT          728 a          617 c          455 g          917 t
ORIGIN      
        1 aaaccatttt atggacgggt tctgcaaagt cataatacat ttcatatacg cctttttcat
       61 ctgcttgctc atccttgaca ttgctgacat acataccttt gttatacgta taagaccgaa
      121 tccaagtccg aaattttgca ttatcgctga cttgttcagc aataatctca tgagcaccgt
      181 gcaacgcttc ttcggcagaa gagacttctt tttctttgtc aatatatttt tctgcttcag
      241 attgcacctc tccatctgta agctgcatca gccacttcgc gagtggttca agtccttttt
      301 ctttagcaat cgttgctttt gtccgtcgtt tttgtttgta tggacgatac aaatcttcca
      361 cttgttgcat tttcacggct tgggtgattt ctgcttctaa ctcgggagtc agtttccctt
      421 gttcatcgat tagccgaatc acttcagttt tacgtttttc taagttttca agataggcat
      481 accgctcttc gatttcacga atctggactt catcaagact tccagtcatt tcttttcggt
      541 aacgcgcgat aaaaggaaca gtgtttcctt catttaacaa attaagtaca gtagtcaact
      601 gcttaggacg atagtctgaa agttcttttt gtacaagctg aatgatcgtc tggttcaaat
      661 tttccgtcat taaaatgcct cttttctgct aaaaattgct tctttattct atcatacttt
      721 ttatcctggg ttaaactact gaaagaaaca atattctcta taaaaataaa aagagccgca
      781 acaaagtcac agctccttca ttctttccat tagtcagatt ccatgccaag gtccttcgtt
      841 gttctgatat tcaatatctt gaagaatacc ttccttgtca aaaacaaccc catgcaatga
      901 ccctccgaat acagccccac cgtccattcc gattttatga tcctgaatcc agaggtctgt
      961 tgtctgcata tctccatgga gcattggtgt aatcgtatgt ccaaatacaa ttgtcttacc
     1021 tgtattgttt ttcccttcat gaaatggttc ccttatccaa ataaagtctc ttagacttgt
     1081 cttacgccag tctttctttg tcaaatccac accggcatgg acaaaaagat aattttccca
     1141 ttcataatac aaggggcgct ggactaaaaa tttaatcagt tctgaatatc tcgattggat
     1201 catcatcgag atttcagttg gggagtattc ctccgatgcc cctgggtgaa tcaggctttc
     1261 aaacgtttct tttccaccat ttctcatata aggcgcatac cagtcctccg gtgattgcag
     1321 aaattgcaaa aaatattctt catggtttcc ccggaggtaa atggctcctg tttcttcgac
     1381 taattgcttt cctaataaaa aacattcttt tgtacgagga cctcgatcat tgagatcccc
     1441 taataaaaca agctgatgga tctttggatc ataatagtga ataagttttt caaaaagctc
     1501 atgtttgcca tggacatccc caatagcata aatcaatgat ttcatgtgga tcaccatcct
     1561 caacgctagt atagcgcata ttggaaatgg tcgacaatca aaactattgt tttctattta
     1621 attaaagctg taaaccgcag ttctgcttct tctggagaga ttccatggtc ttgaacatag
     1681 cgattgcgcg gatgtttcgc acactctatg ctacatgctc ctaaatgttt ttcttcattt
     1741 tcaatggaag caagaatctg acgattgcac tcaggattgc tacaattgat atatcgttca
     1801 catggtgttc cgtcaaacca gtcgcgaccg acgatttgtt tatctacttg gttgatatcg
     1861 acagcgatcc gttcatcaaa cacatacatc ttgccgtccc ataattctcc ttggacttca
     1921 ggatccttac cataagtagc aataccgcca tgcagctgtc cgacatcttt gaatccttca
     1981 cggacaagcc aaccagaaaa cttctcgcaa cgaatgccac cagtacagta ggtcactacc
     2041 cgtttttcca taaattcttc tttgtgctcg cggatccagt ctggaagctc tcgaaaactg
     2101 cgtatatctg ggcgtacagc ccctcgaaaa tgtcccaagt catattcata atcattccgt
     2161 gcatcaatca ccactgtctg ttcatctaaa atagcttctt taaattcttg cggtgaaaga
     2221 taggcacctg tcacttggtt tggatcaatg tcctcttcta gctttaaact tacaagctct
     2281 ggacggtatc ttacaaacat cttcttaaag gcatgtcctt cgcttggatc gatcttgaac
     2341 atcgtatcag caaatcgagg atcagctttc attgctttca tataggcagt cgtgttttca
     2401 acggttccag acacagtacc atttattcct tcttcagcta ctagaatacg acctttaaga
     2461 tccaaagatt tgcaaaaggc taaatgattt ttggcaaatt cctctggttc ttcgattttt
     2521 gtataattgt aataaagcag tacttgataa tccatgtatt tcttccttcc ttcttttcgt
     2581 tcctaaacag tataaagaag gcgttcgcat ttgacgattt atttgcttgt gtataattac
     2641 gcatggaaag ttttttgttt acataatatt attatttgaa ttaaaaagac ttcgctttcc
     2701 tgtttcgcac agaaaaa
//
LOCUS       DACEOE010000237         2717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEOE010000237 DACEOE010000000
VERSION     DACEOE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.246"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..779)
                     /locus_tag="IW774_002627"
     CDS             complement(<1..779)
                     /locus_tag="IW774_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1154947.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIM"
     gene            950..1318
                     /locus_tag="IW774_002628"
     CDS             950..1318
                     /locus_tag="IW774_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1154948.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1435..1920
                     /gene="mraZ"
                     /locus_tag="IW774_002629"
     CDS             1435..1920
                     /gene="mraZ"
                     /locus_tag="IW774_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1154949.1"
                     /translation="MGDCGRLILVSGGMGGLSMLMGEFQHNIDAKGRLIVPSKLREEL
                     GEKFVLTRGLDGCLFGYPMSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQ
                     GRINIPSTLRNYAALTKGCVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFG
                     L"
     gene            1941..>2717
                     /gene="rsmH"
                     /locus_tag="IW774_002630"
     CDS             1941..>2717
                     /gene="rsmH"
                     /locus_tag="IW774_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1154950.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFK"
BASE COUNT          836 a          436 c          576 g          869 t
ORIGIN      
        1 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
       61 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
      121 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
      181 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
      241 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
      301 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
      361 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
      421 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
      481 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
      541 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
      601 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
      661 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
      721 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
      781 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
      841 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
      901 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
      961 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
     1021 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
     1081 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
     1141 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
     1201 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
     1261 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
     1321 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
     1381 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
     1441 gattgtggta gactaatttt agttagtgga ggaatggggg ggctttcaat gttgatgggt
     1501 gaattccaac ataatattga tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa
     1561 gaacttggcg aaaagtttgt tctgacacgc ggattggatg gctgtctttt cggctatccg
     1621 atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac
     1681 gcacgtactt ttgtgcgatt cttctattcg gctgccactg aatgcgaact tgataaacaa
     1741 ggacgaatca atatacccag cacgttaaga aattatgcag ctttgacaaa aggttgcgta
     1801 gtcatcggtg tatctaaccg aattgaaatt tgggatgaga cacgatggca agatttttct
     1861 gcagcagcag aagagaactt tgacgaaatt gctgaaagta tgattgattt cggactataa
     1921 gaataggaag aggctgacta atgactgaaa catttcaaca ctatacagtc atgttgaaag
     1981 aaacggtaga tggattgaac atcaaaccgg atggcgttta tgtagactgt acactcggcg
     2041 gtgccggaca tagtgaatat ttattaagcc aactaagttc taaagggcat ctgtatgcgt
     2101 ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg tttagcttct tatgtcgaac
     2161 aaggtatggt cacatttatc aaagcgaact tcagagaatt agaagaacgc ttgagtgagc
     2221 atgtagataa ggtagacggc attttgtatg atctaggggt ttcctctcct cagctagatg
     2281 aagcagaacg tggatttagt tatcatcaag atgcaccgtt ggatatgcgc atggatcagt
     2341 cagctccttt atccgcttat cacgtaatta atgaatattc ctaccatgaa ttagtaaaga
     2401 ttttcttccg ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag
     2461 tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt aaacaagtca
     2521 tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt caagctgtgc
     2581 ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc tttagaacag gcaatccggt
     2641 tattgaaagt caatggaaga atcagcgtaa tcacattcca ttcattggaa gacagaattg
     2701 tcaaaagtat gtttaaa
//
LOCUS       DACEOE010000238         2708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEOE010000238 DACEOE010000000
VERSION     DACEOE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.247"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            240..656
                     /locus_tag="IW774_002631"
     CDS             240..656
                     /locus_tag="IW774_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1154951.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            670..1236
                     /locus_tag="IW774_002632"
     CDS             670..1236
                     /locus_tag="IW774_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1154952.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1259..2245
                     /locus_tag="IW774_002633"
     CDS             1259..2245
                     /locus_tag="IW774_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1154953.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2400..>2708)
                     /locus_tag="IW774_002634"
     CDS             complement(2400..>2708)
                     /locus_tag="IW774_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1154954.1"
                     /translation="DSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSD
                     NRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR
                     "
BASE COUNT          824 a          504 c          560 g          820 t
ORIGIN      
        1 ttgaaaagta attttcgtaa attccttctt atttcttgaa gttgcccact tctgtcacaa
       61 cctcttgtta taaacggtgt tccacctgct gcttctcggt aataagccca aagaattaga
      121 aaacatggaa agctgttttc tattcttcgt tcttattact caaagcaaga cacaggtgtc
      181 acaacctctt gtttttatgc taaaattggg tgagttaatc tggaaaaagg agaacaaaaa
      241 tgagccttaa agaatttgct gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt
      301 tgcttttgac tgttctttct tttttaccag tcatgatttt tggtcttcag caaaacaccg
      361 caacagcaga aaaagaagcg gtactgcgtg tggatggcac agaaatcaaa acattccctt
      421 tgatggctgg agaagagagc aacacatata cttacaaaga tgaacacgga gattataact
      481 tgatcgagat agatggcgat cgagtacgga taaaagaagc agattgcgat gaccagatct
      541 gcgtacgtcg aggctgggca acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat
      601 tagtgatcga agtccgttca acagatggag gtgacgaaga cagtctgatt tattagacct
      661 tcacacacca tgagtaaatt acaaaaaatg atttatattt cgctacttgt tgcacaagga
      721 gtgatcattg gtttattgga aaatatgatc ccttatcctt tttcttttgc gcctggtgca
      781 aaactaggct tagccaatct aatcacgatc gttgccattt ttaccatgaa aaaacaagac
      841 agcttcttac tactttggct acgactattc ctgactactt tgctaggcgg aacgatttcc
      901 acattccttt atagtatgag tggtgctttg cttagttatt ttggaatgtt attggtcaag
      961 caattaggac caaaacgtgt cagtatcatc ggaattagtg cggccggcgg tttcatgcat
     1021 aatgtcggac agcttttgat ggcttctttc attgcacaat catggacagt catgttgtac
     1081 ttgccagtcc tttcagtttt agggatttta tcagggattg caatcgggat cgcagcgaat
     1141 tatcttttac gacatgtgca gacacttcaa cgattccaaa aagattatga gcagggaacc
     1201 aaacaaaaat ggagcccaat gtttaccaaa aaataatcgt cacacaagga gtttttttat
     1261 gaagctacat ccaatgtggg aaaactatcc tgctttaagt aaagaattga cagaaacact
     1321 aaaacggatg gaagaaactg tccgcctgaa aaacaaacct gtcgaacaag ccatcaaaga
     1381 aacgatccat gccggcggaa agctgttgcg tcctgcctat cagctattgt tctcacaatt
     1441 tggaccagag caagatcgag acaaagccat cgctttggca gcttctatcg aaatgctcca
     1501 tacggccaca ctgatccatg atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc
     1561 taatatccgt agtcagtttg gtaatgtcgt ttctgtctat gcaggagact atttatttgt
     1621 ctgctgtttt aaactattgt ctgattactc cacttccttg aaaagtctcc aattgaattc
     1681 tcgcagtatg gaaaaagtat tgaatggcga attagggcag atggatgatc gctataatta
     1741 tgaactttct gtaaaagaat atttggaaaa tatttcaggt aagacagctg aactgttcca
     1801 attaagttgt tctgttggcg cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc
     1861 aggagatatc ggactttcta tcgggatggc ctttcagatt atcgatgata ttttagatta
     1921 tacaaaagag agccaggaaa tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta
     1981 ttctttaccg ttgatctact ctttgcaaaa aaacaaacca aagctactac cttatttaga
     2041 gaagaaagcg gcaatgacag aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac
     2101 aggaggcgtg gaagaagcta ggaagttagc ggcctcttac acagaaaagg ctttgaagga
     2161 aataaagaaa ttacctgcca cctctcttcg aacaaaagag aatctctttt cccttaccca
     2221 actgattttg gatcgcaaag actgaatagg gaaaggttcg atacgtaaat aaatttctca
     2281 gaaggaaatc tacgtgtttt ttgacaaatt gaattgtagt tcttgtattt atgataaaaa
     2341 gcaaaaataa agagcaacgt ccgttagcga cgttgctctt ttttatcttg aatatttttt
     2401 taccttttcg atttcttata actcataaaa gtgattctta aaccgattac aagtaatata
     2461 acgcctgcga gtgttatcca attattcgat tgacttccag ttttaggaag tgctcttttt
     2521 gatttgtttc cattgtcatt gcttgtttta ttagttaagt taaagttcgt cctgttatcc
     2581 gaacttgttt ttgagaagga gacacttgca tgacgtgtat cgcttgtggt agacgacgtg
     2641 tctgtactat cgcttgaagt tgatgaatct gagctatcac ttgtggtaga ggatgtatct
     2701 gtactgtc
//
LOCUS       DACEOE010000239         2705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEOE010000239 DACEOE010000000
VERSION     DACEOE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.248"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            718..2595
                     /gene="ltrA"
                     /locus_tag="IW774_002635"
     CDS             718..2595
                     /gene="ltrA"
                     /locus_tag="IW774_002635"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1154955.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
BASE COUNT         1039 a          416 c          599 g          651 t
ORIGIN      
        1 gtgaaaagct gtgggttgca agtagtctaa aaactattgt aataactctt ataacgagaa
       61 gtcaaatgag tgggtaacgc cacaaagggc ttccactgat actccgacat gcaacaagta
      121 gtttatgcag accaacgttg tgtgaagctc ggtgaagtcg gctgaaggat accgtaaggt
      181 aaaagaaata gtgaatacca cgaaagttct ttagaggtaa agggtgtatc cgataagtcg
      241 ggtgtccaaa aaaacttaat atggtgagta tgtacaaacg tactgacgaa ctagcgaatg
      301 tactgtccga aaggcatagc tcctagaaat gggagagaca gtgagcaaaa aatcactcat
      361 tgtgctaact tgtggttgag taaaagtcgt tgttatgaaa gaccatgctg cgttacaggc
      421 gtaggagaag ttagggggca aaagctagca tctaagttaa ggcatgtgga tagatataag
      481 ggaacagggg aagccatcaa catggagagt gcatactcga tgaagtggta gtaagaaata
      541 tgtgaatgac aaataactta ctttagtgat ggtgaaagta gagtcacgac tgatgaaggt
      601 tctgtaatgg aactggagga acaggctcga ttcgtctgta aaagtcatga aacccgttca
      661 accatgggat tctcgtatga ctaaaaaggt cacgagccaa ttggagcgtg ataaaccatg
      721 acaaaagaaa caagtccaaa agataggaag ttgcggcatg cagaatatta tggcatgacg
      781 gaaatcttcg ataagatata ctccaaaagt ttgaagggag ataatttcaa gaatctaatg
      841 cagattatca tttctgataa caacattgtc ctagcatatc gtaatatcaa gagaaatcac
      901 ggtagtgtga caagaggaga agatggagta acgattaaag atattgaaaa tctcaaccgt
      961 aatgaattcg tagaaattgt taggaagcga ttcgcacatt ataaccctcg gaaagtaagg
     1021 cgtgtagaaa ttcctaaata taatggtaaa ttaagaccgt tgggtattcc atcaatatgg
     1081 gatagaatcg cgcagcaatg catcttacaa gtattagaac caatatgtga agcaaaattc
     1141 aataatcaca gctatggttt ccgcccaaat agatcagcag aacacgcaat agcagattgt
     1201 atggcccgaa tgaatcaaag tcacatggaa tatgtggtag atgtagatat caaaggtttc
     1261 ttcgacgaag tcaatcatac taaactcatg agacaaatct ggtctatggg tatacgagac
     1321 aaacagcttc tcttaatcat taggaaaatg ttaagagcac ccgtagtact tccaaatggg
     1381 aagttgatat acccaacaaa aggaacacca caaggcggaa tacttagccc attacttgcg
     1441 aacatcaatc tcaacgaatt tgactggtgg atagcaaatc agtgggagga aaaagatgcc
     1501 aaagagctaa aacatcattt taggaaaaat ggagcttggg ataaaggtaa tatctatcac
     1561 aagctcagaa agagtacaac tctaaaagaa atatacctag tgagatacgc agatgatttt
     1621 aaaattttct gtagaaatta taatgaagcg aaaaaagttt tctatgcaac taaaatgtgg
     1681 ttacaggagc gtctaaagtt accaatatct gaagaaaagt caaaaatcac aaatctttca
     1741 aaagaagaaa gtgaattttt aggctttacg atgaaattag tgaccaaagg taacaaaaag
     1801 gttgttaata cgcatatagg tacaaaagca caaagcatga ttaaagtgaa actaaaaaaa
     1861 caaatcaaac gaattcaaaa aagtcctaat agcaaagata cctttatcta tattggagtc
     1921 tataacagta tggttattgg gattcataat tacttcaaaa ttgcgactca ttgtagtgta
     1981 gcaatgcgaa aaattgcaag agaactcaga ccaagttttg aaatacgact acgagaacaa
     2041 ggaatctcaa aagaaggaca atacggaaag tacgaaggat acaagcctta cctacggtca
     2101 ggacaaatac gatatataag agggtttcca atattaccag tagggtatat tcaacacaga
     2161 catccgatgt gtaaaaaaag aaatattaat aaatacaccg aagaaggtag ggaagccatt
     2221 cataagaagc aacaaaccgt agaagaatgg aagatatcgt ggttgtgtag caatccagtg
     2281 cttagtaaga gagcaacgat tgaatttgcg gacaatcgaa tttcaaaatt catcgctcaa
     2341 aagggaagat gtgcagtaac aggtgaggag cttatcttat ctgaaatgca ttgtcatcat
     2401 attatccctt atcatgaaag caaaagtgat agctatgaaa acctagtgat tgttactgag
     2461 gaggttcatc gggtaataca cgctacacaa agtgaaacga ttgaggaact tctaaaatat
     2521 ctaaaattaa atccaaaaca aaaagaaaaa ctcaatgagt tgagattaaa agttggtaac
     2581 gaagaaatat cttaataaat atttgtagga cgggaaaaca gaatgtcttt tatagacgag
     2641 ggaacgccgt atgcggtgaa agtcgcacgt acggtgtgga ctgggggaaa agacggagat
     2701 aatat
//
LOCUS       DACEOE010000240         2700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.249, whole genome shotgun sequence.
ACCESSION   DACEOE010000240 DACEOE010000000
VERSION     DACEOE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.249"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            34..1368
                     /locus_tag="IW774_002636"
     CDS             34..1368
                     /locus_tag="IW774_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154956.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1355..>2700
                     /locus_tag="IW774_002637"
     CDS             1355..>2700
                     /locus_tag="IW774_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154957.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLP"
BASE COUNT          911 a          406 c          460 g          923 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt attcgtttgg tttctaaagg
     1981 tttcaagaat gcagatttta ttgctatatt taaaaaatta tttagtgcgg caggaatgac
     2041 attggttctg acgagtgctt tttggtatcc gatgtttgaa caaatgctct accaaaaaat
     2101 caacaaacct agcgtgacaa atttatatgc tcatgcatct aatgtgttcg atagtctgac
     2161 tgaagctatg aacaacgact taactactta ctcaatggga ctagttggtc tgttatcttt
     2221 atgtattccc ctgattttat ttaaaaaatt aacaaacata gaaaaaaaga tttattatgg
     2281 tacgtgtttg acttggctag ctacaactag tttggtgcca tggtatcttc tgcaaaatac
     2341 gccagccaaa ttgttacaat ttccttggcg gatcttaagc ttgcaaataa tattctcttc
     2401 tctgatatta acgatgatat ttttcaaaaa tagaagatac aataagacaa gagaactatt
     2461 ttatttattt atgactgttt tgttattaat cactttaact gtttctagta aaaaaaattt
     2521 caatcaaaaa ataatttctc agccgggaca tattgttgta aacaaagaaa cagtagaggc
     2581 gtataccact aaaggaatgg attatttttt atatgattat tctcctatcg cttcattgga
     2641 gaataaggag catataataa aacacgaagc aaagattggc agtcaatggg taaaccttcc
//
LOCUS       DACEOE010000241         2690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.250, whole genome shotgun sequence.
ACCESSION   DACEOE010000241 DACEOE010000000
VERSION     DACEOE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.250"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..799)
                     /locus_tag="IW774_002638"
     CDS             complement(<1..799)
                     /locus_tag="IW774_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1154958.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWK"
     gene            complement(977..1780)
                     /locus_tag="IW774_002639"
     CDS             complement(977..1780)
                     /locus_tag="IW774_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1154959.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1926..>2690
                     /locus_tag="IW774_002640"
     CDS             1926..>2690
                     /locus_tag="IW774_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1154960.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFED"
BASE COUNT          870 a          437 c          525 g          858 t
ORIGIN      
        1 ttttccaaag aggatgatct tgtggaagag gctcttcctc aaaaacgtct aatgctgcaa
       61 aggaaagttg ctggttattt aaagcctgca ccagatcttg tgtcttaaca gaagcaccac
      121 ggccaacatt gatgaataca gattttggat caaagtgttc aaacatatgc tgatcaaaga
      181 gcccagttgt ttcctccgtt agaggtaaga tattgactac gaaatctact tcaggaagaa
      241 cttcctttag ttgatttaaa gaaaaagtct tttccattcc ttctgctgga tgaccggatg
      301 tattgattcc atagcagttc atgtctaatg ctttgcattt ttttgcaatc atttttccaa
      361 tatggccagt tccaatgatt agtatttttt tcccttggat agattggtaa tggaccgatg
      421 aacctaacca tttcatgtca ctctgtgcct tttgtgcttg aaaaagacca cgagaatacc
      481 caaggatgat tcctaagaca tgctccgtaa tcgattcact gtggatgcca ctaccatttg
      541 aaagtaaaat atgtttcttt gaaaaagtct caaggggtag ctgatcaaca ccagctgaga
      601 tagattggac ccatttcaag gtagaagagg ctaatagttt ttcttggtag tctgagttcc
      661 agcccaaact gatctctaca ttttgtagat cctctggttg taattctgtt ttaaagatat
      721 aaccaggcgc tatagtatgg atttcttcga taaattcatc acgaaatgga cgatctgcat
      781 aaatgattct ttctgacata gttaatttcc cgcctttctt tactctaagt gtagcgcata
      841 caagaagaac tttgaagcgc cttgcttgtt ttttgagaaa gaaatatttt tcaaataaaa
      901 aaggggctgt gacaaaaaaa tgtcacaacc tctaaaaggg tctattgaag tagaaataac
      961 gagttgtctt tatttcttaa ttgatcgata tttttgccca tcctttgaca gcttgagcaa
     1021 aaataaagat tcctgcaaaa gcaatgaagg cgctagtgtc aaaagaagga agaaagacca
     1081 tcaagattgt tgcaattata gtaggcaatg aaattgaata gatcaatgtt tttaaacaat
     1141 caagaaaagt agcttttgtt agtcgaaagc gtgcatagag aaaagcagca atgttggtaa
     1201 atagcaaagt gatcagcaga ttcagaaaac taggataaat agaaaatata aatgtcagga
     1261 cttttatcca aagtggtata gatgcttcac ttaaaaaaga acggagctgc ttacctgtta
     1321 agttttttaa agattcattg gtgtagggaa gttctaattg gttattgttc aacaaagcag
     1381 aagtcgtgcc tgtattagga agagctatga taagtttgtt ttttaacatc ccgacactaa
     1441 caaaattccc catcaaatca gtagagatgt cctgttctgt tcgttttccc tctggatcaa
     1501 atgtgaagat aatggagttt gtttggtaaa tgaatccttc tttttcttcg gtttgaagct
     1561 gcccgttttg gattgtaaaa tcaggaatgc gagtagcaat cttttgccca tccaattgaa
     1621 tgttgtttaa gacttgaaaa acttgaaaag agattggcaa agccataatt atactcaaaa
     1681 ctaaaagata gacaatggct ttccaaaatg ggacatttct cgcctccttt aggtctttga
     1741 atcgtataaa ggaactgata attaattgtt ttgttgtcat aagtactcct catctagtag
     1801 tgtcatcact tgcaattcta gcgaagtatc aagtgaaata caagtgcttt aaggaaacag
     1861 ctgaatgatt ggtgaaatat caaaaaaatg ttattcttgt ctaaagacta tccaatataa
     1921 ataaaatgga ggaaggaata gtgcctacac agccacagag atcatcaaaa aaactatttg
     1981 gagtagtggt tttgaatgcg gtgatcactt tatcagaatt tattggaggg attatgtcag
     2041 ggagtttgtc tcttgtttca gatgctttcc ataaccttag tgatacattg gcaattattt
     2101 tttcttacgg ggcacaaaaa atggcccgaa aagaagcaaa taaaaaacgt acgtatggct
     2161 accagcgttt agaaatattg agtgctttca tcaactcatt catattgatc atattatcct
     2221 tgtttttaac cgttgaagca ttcaagcggt tcacttcacc agaaaagata aatagtcatt
     2281 tgatgctaac agtagcagtt attggattgc tcgcgaattt gttctcagta ttattattac
     2341 gacaagaagc agatgaaagt ttgaatataa aatctagtta tttgcatttg ttaagtgata
     2401 cactgtcttc ggtaagtgtg attatcggag caattttgat ccgttttttc gatatctact
     2461 ggatcgatcc agttattaca ctgatcattt cgatatatat attgatagaa gcgataatgg
     2521 tgatcaaaaa agctgccgct attttgatac aaagtgcacc tgctatcgac tatgaaaaaa
     2581 tggaacaaga aattgaagca atcgatgggg taaaggatgt tcatcatgtc catatttggc
     2641 agtatagcga aaagataatt attttcgacg gacatattga ttttgaagat
//
LOCUS       DACEOE010000242         2689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.251, whole genome shotgun sequence.
ACCESSION   DACEOE010000242 DACEOE010000000
VERSION     DACEOE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.251"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..170
                     /locus_tag="IW774_002641"
     CDS             <1..170
                     /locus_tag="IW774_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1154961.1"
                     /translation="QAIARDLAKAGGNINQIAKWCNTHQHDVASGDAQRLSENLKIMQ
                     KELQKIWQQLR"
     gene            152..1066
                     /locus_tag="IW774_002642"
     CDS             152..1066
                     /locus_tag="IW774_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1154962.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            1306..1995
                     /locus_tag="IW774_002643"
     CDS             1306..1995
                     /locus_tag="IW774_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154963.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2115..2201)
                     /locus_tag="IW774_002644"
     CDS             complement(2115..2201)
                     /locus_tag="IW774_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1154964.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
BASE COUNT         1001 a          494 c          518 g          676 t
ORIGIN      
        1 cccaggcaat cgcgcgcgat ttagccaaag ctggagggaa tatcaatcaa attgctaagt
       61 ggtgcaatac acaccaacat gacgttgctt ctggtgacgc tcaacgttta tccgaaaacc
      121 taaaaatcat gcaaaaggag ctacaaaaga tatggcaaca attaaggtaa gccgaaccac
      181 ttcttgtagt cgtgcaatta attacgcaga accacgagca accattaaaa caggaataaa
      241 ctgtgacgtc gattatgcca aaagtgaaat gaaacaaatt cgcatgctgt atggaaaaga
      301 cgaccgtgta caggcacact tattgattca atcttttcga ccaggagaaa ttaccgctga
      361 aaaagctaat caactagggg cagagtatgc aaaaaaaata gcccccgatc accaaatagc
      421 tatttacaca catacagaca aagaccatat tcataatcac attgtcatta actccgttaa
      481 tctggagact ggaaaaaaat ttcaagctca tggtcaagca tttctaaaca aatgttatga
      541 cctaaatgac gagatttgtc ttgcacataa cttaccaatc actgagcgag gaaagaaacc
      601 agaaaaacga accatgtctg aaataaaatt aaaagaaaaa aatgaacctg tttggaaaga
      661 cgaaattcgg attgcgatcg atcaaacaat gaaaaacaaa aaaaccagaa cctatgacca
      721 attttgtaat tccctcaaaa cattggggat tcattgcttc aatcgcggga aaaatttcac
      781 ttacgaattg atccacaaaa agaaaaaagt acgatccaac agactaggaa aggactacga
      841 aaaggagacg attttacatg agctggacag acgagaacga acctataacc aacagcgaac
      901 caacgagcta cgaagagctc gagctagcat tcgcgaaact gcagaagaag tacgaagaga
      961 acaacgaact cttacgcctg aacaaccgga aattagaccg gctcgaagaa accatgaaca
     1021 acacgttgaa agaggaacag accaccaaca agaattatcg ctctaaaacg ttaaaagaca
     1081 tagaaaacga aataaagaaa caacgtgctt ttctcgttcc ttatacgcaa gaaaggatac
     1141 aaactacgat tgatttacaa gttaaaaaac tccatcaaga gctattagac cagcgaaata
     1201 aggaacagaa aaaggtgctt attcaacgat cattggattt tcttgccccc ttatgttgct
     1261 tagtcctgac actgttcgca ttttggttag tatggggagg ctaaaatggc attttattta
     1321 ccctatctac tcatttttgt tagtatttca ggaagtattt ggctcatcta taaaattttt
     1381 caaacaagat attcattaaa aggctctaaa attcgattta agcgattttt ctttcttggc
     1441 tgtatatttt ccttgataat cgtttcaagc ggcttattcg gcgttttaga gggaaataag
     1501 cgcgtttcta gaagtatttt attaggaaat gtcactcaaa agtatgaatc agcgcgtgat
     1561 aaaaagaaaa aagagcaagc attagcacaa aaaatggaag agtttaccac ttgttatgag
     1621 gaaatgaacg atatttttgt gaatcaagaa aaacgtttga cggataaaaa catggaaaaa
     1681 ctcactcgtc tttatcaaaa cttgccagaa aaacagcaaa aagaggttca agacaactat
     1741 gagcgaacca aaaaagacgt gcaatatgta aaagatacaa agatagaaga agcctgtagt
     1801 gatctatttg gagacacaaa cccttggttt gccagtgaag aagaaaaaaa agaaaaacaa
     1861 caatctgtca catatgaacg ctatgaaatc ttacttcaac aagcaactaa catgcaaaat
     1921 ccgacaaaaa aagaaaccgc cttaaactat ctaaaaagtg tcaaggaatg gttagatcag
     1981 caacaacaaa attgaatttg atttaaaatc atgttaaact gaatgtgcaa atgatcaaac
     2041 attagataca cgaaagcccc agagaggtcg gattctctgg ggcttttttc aggttgatgt
     2101 cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa taaagagaca
     2161 caaactccta caagaagggg gcaaatgaac agttcaaaca ttagatacac ctccttccag
     2221 tcgcaagact gtcggatagg cgacaagtta ttatatctta aaaaattaaa tttgtgtggt
     2281 gtttttaagt atcacttgtt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc
     2341 ttctcgacta gattaaggga aattaatcaa gaattgtctt agaacggctt agacaagctt
     2401 tcagagtctc acacctcctc tttgaacaac ggttttagca aaggagccgg aggcgactgc
     2461 gcagtataat atgagagcat agcgatcaca cctttttagc gaccgtaggg agcaagtttt
     2521 ccagtttaga aaaattcgcg aacctttttg gaggaaaaag taggagtttt ttaggcggga
     2581 aattcaatgt gctcttaaga tatactgtta gatatactgt tagatatact gttaataata
     2641 actgttaggg attagcaacc ttatgggaga gtaagtattg acataggta
//
LOCUS       DACEOE010000243         2686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEOE010000243 DACEOE010000000
VERSION     DACEOE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.252"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..395
                     /locus_tag="IW774_002645"
     CDS             <1..395
                     /locus_tag="IW774_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1154965.1"
                     /translation="GKFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENP
                     YYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFD
                     KMSSQGLEEDENVISLAEIAFPNEKIFR"
     gene            411..1451
                     /locus_tag="IW774_002646"
     CDS             411..1451
                     /locus_tag="IW774_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1154966.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            1470..2555
                     /locus_tag="IW774_002647"
     CDS             1470..2555
                     /locus_tag="IW774_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ1154967.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            2588..>2686
                     /locus_tag="IW774_002648"
     CDS             2588..>2686
                     /locus_tag="IW774_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1154968.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHI"
BASE COUNT          984 a          366 c          516 g          820 t
ORIGIN      
        1 taggtaaatt tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga
       61 aaggcgatat gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca
      121 atgcagaaaa cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg
      181 cacaagtcta tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt
      241 atatcaaaaa atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc
      301 tatttgataa aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag
      361 aaattgcttt tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc
      421 cgaaattcag cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg
      481 atattctaaa agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa
      541 cagatcaaac acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga
      601 ttactacgac caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag
      661 gagaatatct cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt
      721 atgatcattt gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc
      781 gtacaatgga cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact
      841 atctaactca cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt
      901 atgttgtatg gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta
      961 aaacttataa tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc
     1021 aagtgatcat gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca
     1081 ctaatcaata ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata
     1141 ctgtaacaga cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt
     1201 cagttatttt ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag
     1261 caaagcagat attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt
     1321 cttcagcaaa aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg
     1381 ctgtaaaagt tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc
     1441 gagtatatta gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa
     1501 tgggtttagg tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa
     1561 actatcaatt tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag
     1621 agattgaaag attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta
     1681 aatatttaat agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc
     1741 aggtttattt tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga
     1801 gaattgctca tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat
     1861 tgcgtaaatt tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc
     1921 aagaagctgg tgaaagtctg tttcaaaacc atccctttga gatagttcat aatggtattg
     1981 atttagagct ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata
     2041 tctcaatgag tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat
     2101 cttatttgtt agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt
     2161 tagtaggtga agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag
     2221 agaaaagagt aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg
     2281 atgtttttgt aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag
     2341 cagcaaaatt aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata
     2401 atgtggattt tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag
     2461 agccgtatgc taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg
     2521 aacaaatgga gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg
     2581 attatttttg aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag
     2641 attggtcaat cgattgaacg aaaatgcagt ttttattatt catata
//
LOCUS       DACEOE010000244         2684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.253, whole genome shotgun sequence.
ACCESSION   DACEOE010000244 DACEOE010000000
VERSION     DACEOE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.253"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            32..2629
                     /gene="adhE"
                     /locus_tag="IW774_002649"
                     /gene_synonym="adhC"
     CDS             32..2629
                     /gene="adhE"
                     /locus_tag="IW774_002649"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1154969.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT          923 a          509 c          584 g          668 t
ORIGIN      
        1 caaataacca tgacccatta ggaggataat gatggctaaa gtaatagaaa aagaaaaaac
       61 gaagacaatt gacgtacaag caatgattga tgatttggca gaaaaagcaa atgcagcatt
      121 gaaagaaatg gaaagctttg atcaagaaaa agtagaccat atcgtgcatg aaatggcaat
      181 ggcagctttg gatcaacata tgccattagc gaagatggct gtagaggaaa caggccgcgg
      241 tgtttacgaa gataaagcaa tcaaaaatat gtatgcgtcc gaatatattt ggcataatat
      301 caaacatgat aaaacagttg gtgtgatcaa tgaagatgtc caaaaagggt tgattgaaat
      361 tgctgaacca gtcggtgtgg tttgcggagt aactccaaca acgaatccga cttccacaac
      421 gattttcaaa tcaatgatcg cgttgaaaac aagaaatcca atcgtttttg ctttccatcc
      481 aagtgcccaa aaatcttctg cagaagcagc acgagtggta cgtgatgctg ctatcgctgc
      541 cggagctcct gaaaattgta tccaatggat cgaacatcct tcgatcgaag cgacttctat
      601 gctaatgaac catccaggta tcgcgatcgt cttagcgaca ggtggagctg gaatggtcaa
      661 gtctgcttac tctacaggaa aaccagcttt aggtgtaggt ccaggtaacg taccagctta
      721 tattgaaaaa acagcaaaaa ttaaacgtgc agtgaacgat ttgatcgttt cgaaaacatt
      781 tgataatgga atgatttgtg cttctgaaca agctgtgatc gtggataaag aaatctatgc
      841 agcagtaaaa gcagaattcc aagcacacca agtttatatc gtaaaaccag atgaattgca
      901 aaaattagaa gatgcagtga tgaacgaagg aaaatatgcg gtcaatccat caatcgttgg
      961 acattctgcg atggaaatcg caaaattggc aggaatcagt gtaccaaaag gaacgaaaat
     1021 gctgattgct gaattggaag gcgtaggccc agattatccg ctatcaagag aaaaactttc
     1081 tccagttctt gccatgatca aagctaacaa cacggaccat gcgtttgatt tgtgtgaagg
     1141 tatgttgaat cttggaggat taggacatac agcagttatc cattcagaaa acgaagagtt
     1201 gcatgtcaaa ttcggtcttc gaatgaaagc ttgtcgtatt ttagtgaata caccatctgc
     1261 agaaggcggc atcggtgata tctataacga aatgattccg tcattgactt tgggatgcgg
     1321 ttcttatggt aagaactctg tttctcgtaa cgtctctgct gtaaacttaa tcaatgtcaa
     1381 aactgtagcg aaacggagaa ataacatgca atggttcaaa ttaccgccaa aaatcttctt
     1441 tgaaaagaac tctctattgt acttagagaa aatggaaaac gttgaacgtg tcatgatcgt
     1501 ttgtgaccct ggtatggtcc aattcggtta ctgcgataca gttcgtgaag tattggcacg
     1561 acgtaaaaat gatgtgaaga tcgaagtctt ctcagatgtt gaaccgaacc catcgacaaa
     1621 taccgtatac gcaggaacga aactgatggc agacttcaag ccagatacag taatcgctct
     1681 tggaggcggt tctgcaatgg acgcagctaa gggaatgtgg atgttctatg aacacccaga
     1741 cacatctttc tttggtgcaa aacaaaaatt cttggatatc cgtaaacgga catataagat
     1801 cgacaaacca gaaaaaacac aattcgtatg tatcccgaca acatcaggga ctggttctga
     1861 agtaacgcca tttgcggtca tcactgacag tgaaacacat gtgaaatatc cgttagcaga
     1921 ttatgcattg acgccagatg tcgcaatcgt cgatcctcaa ttcgtcatgt cagttccagc
     1981 atctgtcaca gcagataccg ggatggatgt gttaacacat gcaatcgaat cctatgtttc
     2041 agtcatggct tcagattata cacgtggatt gagcctacaa gcaatcaagt tagtctttga
     2101 ctatttagaa aaatcagtaa aaacacctga tatggaatca cgtgaaaaaa tgcacaacgc
     2161 ttctacaatg gctggtatgg cgtttgctaa cgcattccta ggtatttgtc actcagtcgc
     2221 acataaaatc ggcggggaat acgggatccc tcatggacgg acaaatgcga ttcttttacc
     2281 gcatattatc cgctataatg caaaagatcc acaaaaacat gcgatgttcc ctaaatatga
     2341 ctatttccgt gcagatacag attacgcaga tatcgcaaaa ttcttaggtc tgaaaggcga
     2401 aacaacagag gaattagtcg aagctttagc aacggctgtt tatgaattag gaaaatctgt
     2461 cggcatcaac atgagtctaa aagctcaagg agtcacacaa gaaacactag atacaaccgt
     2521 agaccgtatg gctgaattag cttacgaaga ccaatgtacg acagctaatc cgaaagaacc
     2581 attgatctct gaattgaaac agatcatcat tgatgcttac aacggataag caaaagaaag
     2641 cgtagaaaaa agataagaaa atagatgaaa aagaacttgg aaaa
//
LOCUS       DACEOE010000245         2640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.254, whole genome shotgun sequence.
ACCESSION   DACEOE010000245 DACEOE010000000
VERSION     DACEOE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.254"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            227..1198
                     /locus_tag="IW774_002650"
     CDS             227..1198
                     /locus_tag="IW774_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1154970.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            1230..2627
                     /locus_tag="IW774_002651"
     CDS             1230..2627
                     /locus_tag="IW774_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1154971.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
BASE COUNT          944 a          415 c          592 g          689 t
ORIGIN      
        1 agaaagcgtt ttaaaaaagt gttgcaatct tttaaaaaaa tgctatatta aaaatgaacc
       61 ggttcaaata cttgtttaag tataattaat agtgagagga agttgctcta ctgaagactt
      121 tagttatatg gatattgatg acaagcaaca ctatgcaaag aatgcggtca tcataatcga
      181 acaacaagaa agagaaatca aatgtaactg gcaggagtga gaatgtatgg catcgatccg
      241 tgatgtggct aagatggctg gtgtttcagt gggaacagtt tcccgttact tgaatggtca
      301 gcaactgaag gaaaagaaca tgaaaaaaat agcagaagcg atttcagcgt tggactataa
      361 agaaaacatc attgctaaag ggttgaagaa taatcgaagc ttctctattg gacttttgat
      421 aaacgggatt tccagccgtt ttgggtcgga agttgtctca gggatcgaaa gagtagctga
      481 agaaaatggg tatagcttat tattaagcgg gtttgccgat cagccagaaa aaattgaaca
      541 aaaaattgaa tacctgatga agcatgtgat cgatgggctg attgtcttct tatccgaaga
      601 agagtggtct ggatttgaaa tgctcacaaa aatgagtatt ccagttatcg cattgaattg
      661 tccagaagga ccagcaggta ttgatacaat attagtgaat gatcgagaaa gtgtagcaaa
      721 agtgatcgct catcatggtg aacaaggaca taaaaaaatc ggatttattg ctgccactca
      781 aacagactac gtagcaagag aacgtttagc aggagccaaa gaagcggtcc aaaatgatcc
      841 atcgatccaa ttagaggtct ttacaggaga ctattctaga aaaagcggct attacggggc
      901 aaaagcatta ttagaaaaag gggcaactgc tatttttgtc agcaattaca atatgtcgat
      961 tggtgccatc gaatgtttca atcaagaagg tgtcagaatc gggaaggata ttgcgttcag
     1021 tcactatgat tatttagata aaaacgatct gagtatcctt ccgaaaatca cgatcacgcc
     1081 accaacagat aaaatcgggg aacgagcagc agaacagctg ttaaaacgaa tcaaaaaaga
     1141 agaggaagaa aatgaaaaag tgattgtgat ggaaaatatc atacatggaa tcaactaaaa
     1201 cgaactaaaa aagaaaaaga ggtgctttga tgaaaaaata tcaatttcca gcaaattttt
     1261 ggtggggttc tgcagcaagt ggcccacaaa cagaaggaag gatttcaggc gatggaaaag
     1321 gagagaacat ttttgattat tggtacaaaa aagaaccgca aaagttcttt gatcaagtag
     1381 gacctgaaaa aacctcgcaa gtttatacga agtatcaaga ggatgtacag ttgatgaaac
     1441 agactgggca taattcattt cgaacatcta tccaatggag ccgtttgata cctgatgaat
     1501 ctggaacaat caacgaagaa gccgttcgtt tctatcatcg ttattttgat gagttgattg
     1561 aaaacggtat cgaacccttt gtgaatctgt atcatttcga tatgccgatg tatctgcaag
     1621 aaaaaggcgg atggttgaac cgagaaacgg ttgaagccta tgaaaaatat gccaaaagtt
     1681 gttttgaatt gtttggttcg aaagtaaaaa aatggtttac tcataatgaa ccaatcgttc
     1741 cagtcgaagc aggttattta tatcgatggc attatccaga agagtcagat atgaaaaaag
     1801 cgatccaagt tggctatcat gaagcgttgg cttcagcgtt agccatcaaa tcttaccatg
     1861 aaatggagca aggtggtgtt attggtatca tcttgaatct gacaccaagc tatccaagag
     1921 acgaatcaaa ccctgaagat gttaaagcag cgcaaatagc agatgcgttc ttcaatcgtt
     1981 cattccttga tccagctgta aaaggcgagt ttccaagtga attgattgag atagtaaaag
     2041 aacttgatct catgcctgtg atccatgcag aggatttgac ggttatcaaa gaaaatacag
     2101 tagatctttt aggtatcaat tattaccagc cacgccgggt aaaagcaaaa gaaacaccaa
     2161 tcgatactaa aaacggtcca atgcctgagg attatttcga caattatgaa atgcctggtc
     2221 gtaaaatgaa tccttatcgc ggatgggaga tctatgaaca aggaatctat gatattttga
     2281 tcaatgtacg agatcattat ggaaatatcg actgctttat ttctgaaaac ggtatgggtg
     2341 tagaaaacga agcacgattt atcaaagaag atggcatgat ccacgatgat tatcggatcg
     2401 aatttgtcca atctcatctg aaatatgtcc atcaagccat tcaagaaggt gccaattgtt
     2461 taggctatca tatgtggaca tgtatggata actggtcgtg gaccaatgca tataagaatc
     2521 gatatggctt tatttcagta gacttagcac aagatggaaa aagaacaatc aaaaaatcgg
     2581 gtcattggtt caaagacgta tcagataaaa acggatttga ggcttaagaa agaatagttg
//
LOCUS       DACEOE010000246         2627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.255, whole genome shotgun sequence.
ACCESSION   DACEOE010000246 DACEOE010000000
VERSION     DACEOE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.255"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(573..1130)
                     /locus_tag="IW774_002652"
     CDS             complement(573..1130)
                     /locus_tag="IW774_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154972.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IW774_002653"
     CDS             complement(1309..1920)
                     /locus_tag="IW774_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154973.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IW774_002654"
     CDS             complement(2033..2290)
                     /locus_tag="IW774_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154974.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
BASE COUNT          782 a          554 c          345 g          946 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatg
//
LOCUS       DACEOE010000247         2596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.256, whole genome shotgun sequence.
ACCESSION   DACEOE010000247 DACEOE010000000
VERSION     DACEOE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.256"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(857..2092)
                     /locus_tag="IW774_002655"
     CDS             complement(857..2092)
                     /locus_tag="IW774_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1154975.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT          810 a          417 c          516 g          853 t
ORIGIN      
        1 caccgtttat tcggaatatg ggactgtgac aaggcttttg tcacagccct tttttacata
       61 aagattatta ttgttttaac tgttttggca gaaagaagga ggaaaaaatg gaaattttat
      121 cagcaatgga tttagaatta gtagatctac taccaaagtg atcaagtcaa atatagattt
      181 gggagcttac aaccatatag taggggctgc agtatggtgt atgaatggta atatgtatga
      241 tgtgagtgtt tattcaaatc atgaaacgtg tatagaaata attgtgctgg gaatagcaat
      301 cagtcatggt gaacgtgact tttagtgtat cgttgctgac gacatagaac attctgacat
      361 gatctactca atatgtgggg attgctggca gtttatatag gagtatgcat cggattgcga
      421 gatcattgtc tcaacagaag agggaggttg attatcaaag ataaagtggt aaatcctaca
      481 tttgatttag taattgttga ggggactagc ggcacaaatc tctacaaagc gatgcttcta
      541 gatctatatg acacatttta agaaaatgta tttgcctaat tattatggtc tttcttttga
      601 agaaacgttg agatgatcca accagagaaa ggtgtcaaag aatttgacta agaataaatg
      661 gcagacgcta cctatgctca tttggatagc gtcagatctt ttattttaag gatgattttg
      721 tgttttttag acaccgtaat ttaagtaact ctaacttttc aatccactat ttactttttt
      781 tgtattttca ctgtctgggt ctcgaatagc gaaattactg atgagtctct attaaaaaag
      841 ctttttctac ttgaaactat attgggtggt tgctcaggaa ggtttcgatt tcttgaattg
      901 cttgttgtgg accatttatt ggaaaaacgg cttgcagcgt gcgtgcctgc tgacgaatct
      961 tgggtgaaga aacttcgcgc agagcaatct gcagttttct ttcagtcact ggttttgtga
     1021 gggcgattgc gtttccgtat tttacacaat agtcaatatt acagctttgc tcaaattgca
     1081 tccccacacc gataaacggt ttgccggatt cacaggcagt ttggacggtc ccttccccgc
     1141 cgtggataaa agaaaaatca atgaattcat gcaaaagatg actagacaga tattctgtta
     1201 ttaaaagatc ttctgagtca gattgaatgt catcaagcag atagtcaata ggtgcaataa
     1261 ccgctagatc cggttgtttt tgtaagaaga acaaaatttt gcgtaacata cgcggattgc
     1321 ctgaactacc cattgcgaaa tagataatac gttttctttc ttgttgcttt cgctgtataa
     1381 aagttagcac ttcttcaggc aaggaagtac tgagcttagc aaaaattgga ccaacgattc
     1441 gataattttc cggcagctgt tcttcttcca aaaatagttt aggcgttgtg atgagattta
     1501 gatcagcatc catcatttgt aaacttgttt ttggtaaagg aagcatttct ttttgtgcag
     1561 cgtgtttgaa ggcattgggg atccatcggg tatttactat acgctttttt gtccagtgcc
     1621 aaaggggatt gcttaaaggg ccgaagaaac gtaactcgtg gggcatcagc gcatgctctg
     1681 attgaaaaaa aggttcactc aaagcatagg gtttaacata tactaaagga atctttttat
     1741 tgcgggccga taaaaaaatt gtcacattcg aacctgtcac aatcagtcga ggctcaaact
     1801 tagcaatcag actctgttca ctagtgatac gagaggcaac catttctttg gtaaaagggt
     1861 ttttaagtgt acgcaactgg tcaaatttta tgatttgttc ttcttgtttt ttcgtaagga
     1921 tcggcgctag taaatgatac tcaaagccac tttctgtgat caaaggagca aaagttttag
     1981 aaaagccaaa gaatacacag taataacggt ctttcatagc tttggcaacc tcaatcattc
     2041 gtgttgtctc agctaaattg aatactgcag gagcaaaaag aattgtcttc atgaaatcct
     2101 ccttattgga atgagtctat cccttcgttt cttactagta tactaaaaaa cacagcgttc
     2161 gagtagccta tgtatatttt gaatattaaa ctgtctctcg ctgttttcac aacttctaaa
     2221 tttaaatttt gtaatcaagt tgaaactcca attttcaaaa aaacacgcaa caattttttg
     2281 atcgatttat atgttgtgcc agataaatac gagaagttgt gtcattaaaa ataaacatga
     2341 gaacataaaa aaataaaata attatattaa ttgataggtg tgctgtcgtc attatacaca
     2401 cgtaagaaag aaatagttat gtttaaaaat attcttagac tttctcgcca tgacaatttt
     2461 cactatgtgt tataactgta aacgataatt attcagataa cacaaaaaga aatcaaggag
     2521 cagatacgcc agttgtttaa tttgaatatt attttaaaat taaaattttt attctgttct
     2581 aaacattttt ggttgt
//
LOCUS       DACEOE010000248         2546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.257, whole genome shotgun sequence.
ACCESSION   DACEOE010000248 DACEOE010000000
VERSION     DACEOE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.257"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..216
                     /locus_tag="IW774_002656"
     CDS             <1..216
                     /locus_tag="IW774_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1154976.1"
                     /translation="SEMETVRSATENLFSHICAKRIGETIADLVEEATLTQEDINEIL
                     KQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            229..438
                     /gene="copZ"
                     /locus_tag="IW774_002657"
     CDS             229..438
                     /gene="copZ"
                     /locus_tag="IW774_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1154977.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            438..>2546
                     /locus_tag="IW774_002658"
     CDS             438..>2546
                     /locus_tag="IW774_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1154978.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPII"
BASE COUNT          853 a          457 c          589 g          647 t
ORIGIN      
        1 tcggagatgg aaacagttcg cagtgcgacg gaaaatcttt tttcccatat ctgtgcaaaa
       61 aggattggag agacgattgc ggatctagta gaagaagcca ctttgactca agaagatatc
      121 aatgagattt tgaaacaact ggaacaaaag caaccagtag atacgatcga atgtaactgt
      181 atcccaggtc aatgtgaatg taaacaacat caataggagg aagaaaaaat gaaacaacaa
      241 ttttcaatcg aaggtatgag ctgcaatcat tgtgcagcaa gagtagaaga aacagtaagc
      301 gcacttgacg gcgtgcaaaa agtgaaagta aatttgaaaa aagcaaacgg tacggtcaag
      361 tttgatgaaa caaaagttca atccgaaaag atttgtcaag caatcaatgg attgggttat
      421 aaagcagagg tgatttgatg gcaacagcag aaaccaaaat ggatacgttc gtcattacag
      481 gtatgacttg tgcaaattgt tctgctagga tcgaaaaaga gctgaacgga caacctggtg
      541 tggtcaatgc cacagtcaac ttggccactg aaaaagcaag tgtcaaatat gaagggacga
      601 ccactgaaaa attaatccaa agcgtagaaa acatcggtta tggtgcaatt ttgtacgatg
      661 aagcccacaa acaaaaaatt gcagaagaaa agcaagcgta tttgaaaaaa atgctattcg
      721 atcttatatt gagtacggtt ttgactttgc cgttgatgtt gtcaatgatt gctatgatgt
      781 taggaagcca tgcggcgatt gttcactttt ttcattttcc aatcgtccag ttagtgctgt
      841 ctgctcctgt ccaattttat gtcggtgctc gtttttataa aggtgcgtac catgcaatca
      901 agacgaaagc accaaatatg gatgtattgg tagcaattgg aacatcggct gcctttgcat
      961 tgagcattta caacggattt ttccgtggac acccccaaga tctttatttt gaaagcagca
     1021 gtatgatcat cacgctgatt ttgctcggca aatatttgga acatacagca aaaacgaaaa
     1081 caggtaatgc aatcaaacaa ttgatgtctc ttcaaacaaa aaccgcccaa gtgataagaa
     1141 acgggaaaga agaaacgtta gcgattgaag aagtagtcgt tggtgatcaa ctagtcatcc
     1201 gtcctggtga gcagattcca gcagatggtc gaattatttc aggaagcagt gcgatagatg
     1261 aaagtatgct tactggtgaa aatcttcctg tagaaaagaa tccggatgac acgctttttg
     1321 gcgggacaat caatacgaat ggtctgctac acatggaagt cacacaagtc ggcaagcaga
     1381 cagtattagc gcaaatcatt caaatggtgg aagatgctca agggagcaaa gccccaatcc
     1441 aaaaaatcgc agatcggatc tcagggattt ttgttccgat cgtattagtc atcgcgttta
     1501 tcacgctcat agctacaggt ttgatcactg gtgattggca actcgcattg atccatagtg
     1561 tctctgtatt agtcattgcg tgtccatgtg cattaggttt agcgacccca acagctatta
     1621 tggtaggaac gggtgttggt gcacgaaatg gaattttaat taaaggcgga gaagcattag
     1681 aagcagctgc tcacttagac agcattgttt tggataaaac tggaacgatt acagaaggaa
     1741 agcccaaagt tacagatcta gtcggttcaa aagaagtcct atctattttt tatactttag
     1801 aacaagcttc tgaacatcca ctaggtaaag caatcgtgga atacggaaaa ctgcaagaag
     1861 cagcaacata cgatatgatt gattttacag cccacccagg tgctgggatc agcggtacga
     1921 ttaatggagt acgctatttt gcgggtacac gtaaacgatt aattgaattg aacctatcat
     1981 ttgatgaata ccaagaacac gcgttagaat tagaacagca gggaaaaaca gtcatgtttt
     2041 tagctgatga aaagcaagtg atcgggtgga ttgccgtggc tgatcaaatc aaactagaag
     2101 ccaaacaagc aatcaagcag ctccagaata aaggattaga tgtttttatg ctgacaggag
     2161 acaacaaact ggcagccgaa acgattggta aacaagttgg tattgatcca aagcatatct
     2221 ttgctgaagt acttccagag gataaagcag cctacgtaga aaaacttcag aaagatggca
     2281 aaaaagtcgg tatggcaggc gatggaatca atgatgcacc ggccttagca ttagctgatg
     2341 tcggtatggc aatgggaagt ggaacggaca ttgcgatgga aacagccgat gtgacgctga
     2401 tgaatagcag tttggcatcc attgcacaaa caatcgagtt gtctaggttg acattgagaa
     2461 aaatcaaaca aaatcttttc tgggcttttg tctataatac gattggtatt ccttttgccg
     2521 cactaggctt tttgaatcca atcatt
//
LOCUS       DACEOE010000249         2494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.258, whole genome shotgun sequence.
ACCESSION   DACEOE010000249 DACEOE010000000
VERSION     DACEOE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.258"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IW774_002659"
     CDS             complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IW774_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1154979.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTE"
     gene            complement(734..1573)
                     /locus_tag="IW774_002660"
     CDS             complement(734..1573)
                     /locus_tag="IW774_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1154980.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1758..>2494
                     /locus_tag="IW774_002661"
     CDS             1758..>2494
                     /locus_tag="IW774_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1154981.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGS"
BASE COUNT          884 a          505 c          375 g          730 t
ORIGIN      
        1 tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
       61 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
      121 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
      181 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
      241 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
      301 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
      361 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
      421 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
      481 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
      541 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
      601 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
      661 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
      721 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
      781 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
      841 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
      901 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc
      961 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     1021 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     1081 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     1141 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     1201 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     1261 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     1321 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     1381 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     1441 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     1501 tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca atcaaccgaa
     1561 taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg
     1621 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
     1681 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
     1741 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
     1801 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
     1861 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
     1921 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
     1981 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
     2041 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
     2101 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
     2161 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
     2221 ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggggaaaat ttctccaagc
     2281 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
     2341 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
     2401 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
     2461 tagtaaaaat ggtaaaaaaa caggataaag gttc
//
LOCUS       DACEOE010000250         2435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.259, whole genome shotgun sequence.
ACCESSION   DACEOE010000250 DACEOE010000000
VERSION     DACEOE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.259"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(132..869)
                     /locus_tag="IW774_002662"
     CDS             complement(132..869)
                     /locus_tag="IW774_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1154982.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(936..1505)
                     /locus_tag="IW774_002663"
     CDS             complement(936..1505)
                     /locus_tag="IW774_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1154983.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1633..>2435
                     /locus_tag="IW774_002664"
     CDS             1633..>2435
                     /locus_tag="IW774_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1154984.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLI"
BASE COUNT          673 a          515 c          460 g          787 t
ORIGIN      
        1 tttgataaat ttgctttttc tacataaaaa gacgtaaatg aagaatattc ttctccattc
       61 acgccttaaa ttgatggtcg tttgaataac tatttaacaa gtaattctct ttggatatta
      121 tccgttatgg attatacttc ctgtgtcgtc ggacggataa tcatgttact gacagacatc
      181 cggtctggcg tttctatcgc aaatgctaca gcagaagcta tatcttcgga ggtcaatccc
      241 cattcttttt gcgcttcatg aagttcaaga gcagcctttt tgtcgctgat ggtttgatat
      301 aattcagttt gaacagctcc tggagagatg atcgtagatt tgatgttatt ttgccgttgc
      361 tcttgacgta atccttccat gattgcacgg acagcaaatt ttgttccgca ataaacggcg
      421 gaatcaggat aaacaacatg tcccgccaca gaatcagttg aaatgatatg cccagatttt
      481 tgtttttcca tgattggaag tacggcggaa attccattta acacacccat gatgttgata
      541 tcaagcatat tttgccattc atcacggtga ccttcaacta atggagcggt tggcatgatt
      601 cctgcattat tgtacaatac atcgattcga ccgaactttt ccatcgctaa atcgataact
      661 gcttggactt gtgcaaaatc tcttacatct gctgtttgaa tatatagctc agcttctggt
      721 aaagattctt ttattgcttt taggcgatct tctcggcgtg cagctataac tagtttagcc
      781 cctttttctg ctaatagctt cgttgttgct tcaccgattc cgcttgaagc gcccatgatg
      841 acaatcactt tttctgataa atcattcatt ctattctcct cctgttttga aaaatctctt
      901 gcttatactc actatctctt atttcatctt actgttcaaa taatccgttc gacaggtttg
      961 acgattttgg cagggattcc tgcaacgatg gtattgtctg ggacatcttt cgtaacagtt
     1021 gcattcgctg caacaatggc attctcgcca atcgttacgc ctggcagaat agttgctcct
     1081 gctccgatcc aagcattttt cttgatgtgg attggcttta ctgacaagcc tcttcgttct
     1141 tttggatcgg ttagatggtt caccgtgatg aggttgacac gaggaccaat caatactgaa
     1201 tcttctaaac agatgccacc taagtcaacg aacatcactt gacgattgat aaagacattt
     1261 ttccctatac taatatgttt gccgaaatct gtataaaaag gcagcagaac agtcaccgta
     1321 tcatctactt tgtcactaat gatattttct agccgtttgc ggatatctgc tggttcatgg
     1381 tacccagtat tcagcgctgc taaaggctct tcgttttcag cttggatctg atggatcact
     1441 tcaaagagcg gatccgttac tcgaatctct ttattgacga tttgtttttc gatgaggctt
     1501 gtcattttgc tccctccttt tatccttact ttataacgga cggcttatca tgtctaatac
     1561 ttataaaaca tacttaacta ttccttttaa ctatacttag tggtatgctt ttttaaaaac
     1621 aggagggaaa aaatggatat tcgtatactg acgtattttt tagcagtagc tagagaaaaa
     1681 aatatcagcc gagcagcaaa atctctgcat atcacgcaac ctactctctc aaaacaactg
     1741 aaagatttag aagaagaact tggtgtgaca cttttcactc ggggctcccg agagatacag
     1801 ttgacggatt ctggtcgata tctttattca aaaggaaaag aaatcttgtc gttggtagaa
     1861 aaaacggcga acaatttaac aaatgacgca atcatctctg gagaaatcta cattggcgga
     1921 ggcgaaacta aagcaatctc tttcatttct gatagccttc attcgttgat caaggcgcat
     1981 cctgatatcc agtttcattt atatagcggg aatgcagatg atatcataga caaactggat
     2041 agcggattac ttgattttgg actcgtaatc gaaccgacaa acaaacaaaa ataccagtca
     2101 tttcgactgc cagcccaaga tcgttgggga cttttgatac atcgaaatca tcctttagca
     2161 aagcaaaccg tcatccatcc gcaggaactg aaaactgttc ctttgatcat ttcccggcaa
     2221 acgtcagtgg acagtcaact agctgaatgg ctaggtttca gcatagatca gttatcgatc
     2281 gtcggaacat ataacttatt gtacaatgct tctttgatgg tcgataacgg gacttttggt
     2341 gcattagctc tagatgggat catcaatact tatgggacgg aaaaaatatt catcccactc
     2401 gatccgccat tacaagcttc actgaatctc atttg
//
LOCUS       DACEOE010000251         2419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.260, whole genome shotgun sequence.
ACCESSION   DACEOE010000251 DACEOE010000000
VERSION     DACEOE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.260"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(34..624)
                     /locus_tag="IW774_002665"
     CDS             complement(34..624)
                     /locus_tag="IW774_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1154985.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            771..2123
                     /gene="brnQ"
                     /locus_tag="IW774_002666"
     CDS             771..2123
                     /gene="brnQ"
                     /locus_tag="IW774_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1154986.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          723 a          451 c          450 g          795 t
ORIGIN      
        1 ttggagataa aagcttctgt cccagcctct tttctatttc cccggaaaca accgctgata
       61 ataggcatct gcttttttat aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa
      121 taacccgatc aacggtacaa aaaagacacc ggcaatcaag cataagttaa tcatcatgga
      181 gataagactt cgacggtcct ttgacaaaac tctcacgaca tgggaatctg gtttatttgc
      241 tttcatgatt tgcctcatca agataccaaa agaaaaactc cacatcgtgt atactaagac
      301 ataagccatc tcaggccaaa aagcttcagg gttacgcccg acccaactag taacagaagg
      361 acataaagtc agccaaaaga gcaaaaagat attcgaccag accactcggt aattaattcg
      421 ttctgccatc tgaaatatac gatgatggtt cacccagatc accgcaacaa atataaagct
      481 gatggtataa gctaataatg tatttcggat gacccataaa gaatgaaagc tagatgtatt
      541 agggatcttg atctctaata caataactct caaaacaatg gctaccactc catcagtaaa
      601 tgcttctaat ctcgactttg tcacgatgat tcctcctcag atgattcagc gcttttattt
      661 tatcacttac gttattaaat aaccatcgtt ttatataggg aattttctga aaattgttgc
      721 atatttttcg aaattttgtt acttttaaaa aaaccgtaaa ggagttatgt atgacaaaaa
      781 aattatcatt taaagactat ctttttatag gctccatgct tttcggtcta ttttttggtg
      841 ctggaaatct tatctttccc gttcatttgg gtcaagaagc aggcgctgct accttttggg
      901 cgaatctagg tttcttggtt accggtatcg gtcttccctt cttaggggtg attgccattg
      961 gtgtatcgaa aagctcaggt gtatatgaat tagcaaaacg gataaatcaa acatatgcga
     1021 tggtttttac catcttgctt tacttagtta tcggaccgtt ttttgcttta cctcgccttg
     1081 caaccacatc tttcgagatt ggtttagcac catttatcga tcctagcaga cagacgctat
     1141 atctagcaat tttcagtatt gtattttttg tgctggcttg gtggttttca agaaaaccga
     1201 caaagatatt agattatgtt ggaaagttcc tgaatcctgc atttttggtt ttattaggtg
     1261 ctttgcttct ccttgctttt ttgaatccac taggttctgt aaatcaagcg cctattcagc
     1321 caaattatca agagcatgct tttttcacag ggtttactca aggatataac acattagatg
     1381 cgcttgctgc tttagcattt gggatcatca ttgtcacaac aatccaaaac atgggtgtaa
     1441 caaaacctgc tgaaatcgct aaagacacga tcaaatctgg tgcgatcagc attatattga
     1501 tgggaatcat ttatactttg ctcgcttatt taggtgctat gagcttaggt agctttgctc
     1561 taagtgaaaa tggcgggatc acgctggctc agattgctga tcattattta ggtacttatg
     1621 gcagtatctt attagcattt atcgttatat tagcttgctt gaagaccggt attggtttga
     1681 ttacagcttt tgcagaaaca ttcacagatt tgtttccgca aagaaattac gcattctttg
     1741 ttgcacttgc tagtatcctt ccttgtttag cggcaaacgt aggcttgacg aatatcattc
     1801 aattttcgtt gcctgttttg atgttcatct atccattagc aatgacactg attttactag
     1861 tgttagttgg tcctttgttt aagcagcgta cggctgttta tcgaatgact acttatttta
     1921 cattgattgc ttcgattttt gatgggttga acgcttgccc agaaactatc aaacaaacac
     1981 ctattgttca aaatctttta tatgttgcgg aaagctactt gccgtttttc aaattgggga
     2041 tggggtggat cgtaccagca gttatcggtt ttgttatcgg actgatttgg agtttcgcta
     2101 agaaagaaga agtagcagat tgataaaaga ggttgtgaaa gagacgttca gccttgagca
     2161 ataaaaagta attttgaaaa atggcttctc acatttttgc ataaatttga cttaataccg
     2221 agaaactggc ttttgaacac cgtttattga gcgtctataa actgacctga gtattagaga
     2281 aacaaaacgg caaagtagct ccatatactt tgccgttttg ttttgaacta ataccgaaga
     2341 gaagctgatc aaacaacatt tcttttataa tatagatttt agacatatat atgatataaa
     2401 aatagttttc gcaaaaaaa
//
LOCUS       DACEOE010000252         2378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.261, whole genome shotgun sequence.
ACCESSION   DACEOE010000252 DACEOE010000000
VERSION     DACEOE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.261"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(160..705)
                     /locus_tag="IW774_002667"
     CDS             complement(160..705)
                     /locus_tag="IW774_002667"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1154987.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(709..837)
                     /locus_tag="IW774_002668"
     CDS             complement(709..837)
                     /locus_tag="IW774_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1154988.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            complement(830..1921)
                     /gene="phnW"
                     /locus_tag="IW774_002669"
     CDS             complement(830..1921)
                     /gene="phnW"
                     /locus_tag="IW774_002669"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1154989.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            complement(2138..>2378)
                     /locus_tag="IW774_002670"
     CDS             complement(2138..>2378)
                     /locus_tag="IW774_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1154990.1"
                     /translation="LVQMIEDIVLKVERVHFLTIVNTLPDNAHLFASVLDEIKPPSIV
                     STIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
BASE COUNT          695 a          483 c          419 g          781 t
ORIGIN      
        1 ttattacttg atacaggaca gctttttcac aacctcttat tttgtataaa agtcattctg
       61 gattcttgtc agcgtacaaa atgtattctg ttaactcttt taaattgttt acacaaatat
      121 cagcgccggc ttgttggaat ctaagagcaa cagactgttt taaacgatct ttttccccat
      181 ctctaatttt tggtactcat cataagaaat ccccattagc gaactacctt caattacacc
      241 tactgtcaac actccggcat ttttcccttc ctggatatct gagaccgtat ctccgacttt
      301 gattacttca tcaaccgact tgtttttgaa gtactgcatg tttttatgga tcatataagg
      361 aaacggtcga ccataccctt cgacgtcttc aaaagttgcc atgaagtctg gtgaatacct
      421 ttcttttttt gcggtttctg ctacgattgt catcatagga gcattaaatc cattcgtgga
      481 accaatattg atcccagcgt ttctcaaccg ttcgatcgtt tccaatgtgt cgggtttcaa
      541 ggtagaatgc ttgaccaaat ctttaaacag ataatgttca aattgttgat acaattcctc
      601 cacctctttt tcctgaggta gttgtccata aatagctatc cattgttttt tgacagaagg
      661 gagggaaaga agctgttgga tatgatccca ttttaatttt cccatcggtt agcggatttc
      721 tttgtcagtc agttggattc ctttttctag aaaagcattt ctaaaagcgt ggacaggagc
      781 catacagcca aagtccacag ttgttcctgc ccaatcaaaa attacagttt taatcattaa
      841 cgttctcctt atagtctttg ataattgctg tcagttccaa aatatcttcc ggatagatct
      901 cgccaatatt tccgatacga aaacattcat gatcgctgat tttcccggga tagatcacaa
      961 atccattttg ctttaaatac tgataaaaat gattaaaatc aaatcgccag tccttagggt
     1021 aaagaaaaga ggtgatgaat ggcccctgat attcttgaat gaaaggctgg aaccccatct
     1081 ctgtcatttt ttgtcgtagc acatgattat tggcgagata acgtgaatat cgagtttcga
     1141 ttcctccttc tgcctctagt tccttcagcg cttgttgaaa agccaaaacc gtatgggtag
     1201 gggaagtgaa acgccatttg ccattctcgt tttccatcac gcggtactgg tcatatagat
     1261 caagtgacaa acttcggcta acattttttc gtttttccag ttccgcttta ttagcaagta
     1321 caaaagaaaa gcctggaaca ccttgaatac atttgtttga gctggaaatc aaaaaatcaa
     1381 tccctagttc ggctacacaa atcggtatcc caccaaagct tgacatcgcg tccactataa
     1441 acaaaagatc tctttcttgt gtgacagcag caatctcttc caaattattc aaaataccgg
     1501 aagtagtctc gctatggacc attgctagtg cggagataga cggattctct tcaatcagct
     1561 gtgtaatcac atgagcttgg aggatttgat gttccgatac ctcataaacg gtatagttca
     1621 gtttcagaca ttcgcacatg ttgatcattc gttgtctata tgcgccgttt gaacaaatca
     1681 gtacagtatc ttctggcctg atcacagaac ttaataccga ttctaccccg aaggagctgc
     1741 taccttgcat taagaccgtt gtgtatattc cttccgatac ttgagcaaga tccaataatt
     1801 tttttcttat ccattgtgta atttctttgt attcttgatc ccaagtacaa tgatcgatca
     1861 gcatagcatt tttcactgtt tcagaagtcg tcaatggtcc agaagttaat aatttataca
     1921 tatgatttcc tttcatttct tttttgaaat acacaaagag caagaagctg ctcttgttaa
     1981 catccaatat aaatgatgat tattgtgatt caatcaaggt gttgaaaaat ttgaataaaa
     2041 aatgatgtat ttttgtaaag agtgtaaagg tttattcaat aaggaaaata ttgaaaataa
     2101 aaaaacacgc attccatatt aggaatacgt gctctgatta aaaaagtgat acttcagttg
     2161 gatggtgaag aaattcttct actcgttttg cgtcctcctc attttccgag atgaccatga
     2221 tcgtatcgaa tccagcaatt gtactgacaa tagagggagg tttgatctca tctaaaacag
     2281 atgcaaataa atgagcattg tctggcaagg tattgacgat cgtcaaaaag tggactcgtt
     2341 cgactttcaa tacaatgtct tcaatcattt gaaccagc
//
LOCUS       DACEOE010000253         2366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.262, whole genome shotgun sequence.
ACCESSION   DACEOE010000253 DACEOE010000000
VERSION     DACEOE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.262"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..605
                     /locus_tag="IW774_002671"
     CDS             <1..605
                     /locus_tag="IW774_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1154991.1"
                     /translation="PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            739..2082
                     /locus_tag="IW774_002672"
     CDS             739..2082
                     /locus_tag="IW774_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1154992.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2194..>2366
                     /locus_tag="IW774_002673"
     CDS             2194..>2366
                     /locus_tag="IW774_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1154993.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT          938 a          301 c          422 g          705 t
ORIGIN      
        1 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
       61 aaaaaggtac tgtagtagta acatatccag atggatctaa agaagaagta gaagttacta
      121 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
      181 accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca ttaccagacg
      241 gaagtcaaca ccatgggaca gctgataaaa atggtaattt tacagttaaa gttcctaaat
      301 tagaggcagg gacaaaagtg atagtaactg caactgatga atctggtaat actagtgaac
      361 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
      421 gtaagactga caatcaaggt agcaacagca atcaagacaa aaatagaggc aaaagccagt
      481 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
      541 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
      601 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
      661 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt tttatagaaa
      721 ggaaaagaat gtaattaagt ggaaaaaata gtaattattg gttcaaacca ttcagggcta
      781 gccgcagctc gatttctaaa cttaagcgat tcaaattatg aagttattct aatcgataaa
      841 aaatcagaaa ttagttactt ggaatgtggg acatcgttat ttataaaagg agaagtaagt
      901 aatttagaag atctttttta tcaaaataga atgacactaa attctgagat aaaaaagtta
      961 tttctagaga cagaagttga caaaattgat attcaaaata aaaagattag ttgtgtaaaa
     1021 aaagatggta caagattctc tcaagaatat gacaaagtta tattagctac aggttcaaag
     1081 caaattatgc tagatatacc ggggaataat ttagctaata ttttttcgat aaagaatttt
     1141 ccaaatgcaa aagagatttt taaagctttg gatgatagta ctattaaaaa agtaggaatt
     1201 attggagcag gatatattgg agttgaaatt gctgaagcca taagaaaaag agggaaagaa
     1261 gtatatcttt tcgatgttgc agatagagtg ttgtcgactt attacgaccg tagtttttca
     1321 gataaagttg aagaaatact atcaaaaaat ggaatacatc tttgtctatc agaaagtcct
     1381 aaaaaatatt taggaagaaa aaagattgaa aaaatagtca ctacaagtgg tacccaatac
     1441 tctatggata tggtcgtaca agctatagga tttctatcta atagtccaat tggggaaaag
     1501 gaactcttga gagatgttag tggagcatat ctaactaatg aataccagca aacaaatata
     1561 aaagatatat atgctattgg ggattgtgct acaacttttt ttacgtccat taatagaagt
     1621 agtgttgatt tttctatttc taatgctcta agaacagctt atattgcaac tcaacatatt
     1681 aacaatcaag attttacacc ttctggttcc caatttacaa atggcttttc tatatttaat
     1741 tacaattttt acgcagcagg tttgaattta aaaacagcac gattattaag tgagtctata
     1801 gattatatag aaatagaaga tttcattaga cctgcttttt tgagaaaaaa tagtcgtgta
     1861 aaattaagaa ttagctttga aaaaaataca aagagaatag taggtgtaca actttgtagt
     1921 caagaagatt tatctggact attatcaatg ttttctcttg ctatagaaga acaaattaca
     1981 ttagacaaat tgaaaatgtt agattattta tttcatccta gttttagcca accctataat
     2041 tttattttaa aggcagtaac aggattaaac attgaaacat aatcgttact aaatgaaatc
     2101 aagaaatata ctgtattact caattcattg aaaatattta tcataatttt taatagaaaa
     2161 cactattaaa gagaaaaaag agaggtaaat caaatgaaaa aatctttaaa acttgcatca
     2221 gcagttatat taatgtccca actccttata gcaacacctt tccatgtgtt ggcaagcgaa
     2281 aattcaacat acaaaattgc aaaagaaaga accgagacga ctgaaatgag taattccgat
     2341 gtaacagcag agagtagtga actttt
//
LOCUS       DACEOE010000254         2357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.263, whole genome shotgun sequence.
ACCESSION   DACEOE010000254 DACEOE010000000
VERSION     DACEOE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.263"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(86..1252)
                     /locus_tag="IW774_002674"
     CDS             complement(86..1252)
                     /locus_tag="IW774_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1154994.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTEFTVFDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFVILEILPKKIFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFALSMILLLSFLWLL
                     VRKCELTNGQLTFLWAFLAIMCQSFMEETLATISWVPIVFLMLAMATHQYKTTR"
     gene            complement(1258..1947)
                     /locus_tag="IW774_002675"
     CDS             complement(1258..1947)
                     /locus_tag="IW774_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ1154995.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDDVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(2074..2313)
                     /locus_tag="IW774_002676"
     CDS             complement(2074..2313)
                     /locus_tag="IW774_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154996.1"
                     /translation="MNCKESVNWKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKTSSNCQLLLEVS"
BASE COUNT          776 a          443 c          344 g          794 t
ORIGIN      
        1 tcatccatct gcctcctttc agacaaaaca aaaaacactt tttcacaacg aaaaagtgtt
       61 ttctcttatt actattctat ttcatttatc tggtggtttt atattggtgt gtagccattg
      121 ctaacattaa gaacacaatt ggtacccaac tgattgtagc taatgtttct tccatgaaag
      181 attgacacat gatcgctaaa aaagcccaca agaatgttag ttgtccattt gtcagctcac
      241 attttcttac gagcaaccat aagaaactaa gcaaaagaat catcgataga gcaaaaccaa
      301 tcaatccata gtgattgacg agtgcgtgat acgtattatg gttacctaga atctgatttc
      361 ctctatggaa aaagaaaggt ttcatcccta caaatatttg tcctgttttt tcgcctaatg
      421 tttcatagaa tttgcgccaa atttcttctc gtcctgtaaa taaatgaact ttgtctgaca
      481 ttgcagcaaa ataagaaatc ggtaaagcta gaaccgaaac tgctgcatac atccaaaagg
      541 acaagtgtgt gtttcgaaag atcttcttag gtaaaatttc taggataacg aagataacaa
      601 gcgcgtataa tgctgctttc gattggcaaa cccaaatcgt tgccccagca agaatcaacg
      661 ctggtaatga tagcaatcgc atgtaccatt ttccaaatga actagcaaat cctgtgataa
      721 gaagggctaa tgtcattaaa gaactaccta tcgtatttgt attgatccaa atatctgata
      781 atttgttcga tggattccaa atactttgtc cttctggtac tggtatctcg gtaaatactc
      841 gataaaccgt tacgagtaaa aagacataga atactacaag atacaagcta cgatcaaata
      901 cagtaaattc cgtccatttt acaatcaaaa taatggcaat aattgtcgga acgatcagat
      961 caccatagaa agtatctcct cggtagcttc taaaaaaaga aaagaaaagg taaaaaagaa
     1021 tgcctgctgc aatgaagaat agatcgtttc tctttacctt gtacagattc aaaagaaaca
     1081 aacagcctac aaaaaacgtc actaactgtg cagcattatc ataaataaat aatatcgtag
     1141 gaggatttaa ccccaccgtc catattaaat aaagaatgga taccaacagt aacagaaaaa
     1201 tggcactcat tttattgatc aatttttcta caagttcttg aatgtgactc acttccctta
     1261 tttattgatt ttttctacat tgactaattc gcactggatc acatcgcact ttttcatatt
     1321 atttgcttca aagtctaaca gtgtgagtag tggtttttta cctgcttttg gcaaggataa
     1381 aaaggaattg ttttccttag attgaactac ctggctgtaa tcataaattt ttcctctttt
     1441 ggagacttca tactgatact tgtaatgagc atcgattaga tcaatttctt ctccttcgga
     1501 taaatcaatc atatcggata ataaaacgct tggtgtatct acgttataac cagcaagaat
     1561 ataatttcca tttcccattt cagcatctgg ataataagta gaagctactc ctaaactaag
     1621 cgtgtcgcta ttcgctcctt gaaaaacagg gagaataaat cctgtagaag aaaaaacaag
     1681 ttttccagca atttcacctt tgagatcctt ttctaatgcc tgtttttgat tagtaatcag
     1741 ttgtctctta gaaatatccg ttaaactggt taaattatct tgataggttt tagcctgttc
     1801 ctgaatatgg attgtttttg ttacatcatc tactttttca gcgattttgt ccttttgata
     1861 accttgataa gcaaaataac taaaaaggag aaaaacaatg attacgccca cgtaaggatt
     1921 aaataaagtt ttattgtttt tattcatcta tctaccttct atatattaat taatttacct
     1981 acaactaacc aagataaacc acgttaaatt ttactattct ttttattcaa taacaatgcc
     2041 acatcataat tgatcatttt catatcataa tcgtcaagaa acttctaata atagttggca
     2101 attagaactt gttttcacat aagttcgtct caactgttca aaatttttca ttgatcccaa
     2161 tagacaggct ttctcctttc ctgttatctg catatcattg atatattgca tgacagataa
     2221 tcttttaagg tgtttttgtt tcatcatttt tagcaacttt tcttccattt cttttgacac
     2281 ttttttccaa tttacgcttt ctttacaatt catgagtttt cctaccttcc ctgttttttg
     2341 caaaaataaa aaagcct
//
LOCUS       DACEOE010000255         2343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.264, whole genome shotgun sequence.
ACCESSION   DACEOE010000255 DACEOE010000000
VERSION     DACEOE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.264"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(76..1623)
                     /locus_tag="IW774_002677"
     CDS             complement(76..1623)
                     /locus_tag="IW774_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1154997.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1725..2078)
                     /gene="tnpB"
                     /locus_tag="IW774_002678"
     CDS             complement(1725..2078)
                     /gene="tnpB"
                     /locus_tag="IW774_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1154998.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2068..2262)
                     /locus_tag="IW774_002679"
     CDS             complement(2068..2262)
                     /locus_tag="IW774_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1154999.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          657 a          489 c          421 g          776 t
ORIGIN      
        1 taagcgcccc atagaacagt acctgtacgt caaataaccc gacctataga atagaatcgg
       61 gaatgtttgg cgtttttagt tacgagaaaa atgttgttgt acatccttgt tccaaggcaa
      121 cagttgatct aaagtttctg ctgaaagtac gggaagattt ggtatcttat caaatagaaa
      181 ctctagatac tttacaggat tcaatccgtt aagttctgct gttttatata cactcagaat
      241 gattcctgaa gttcgtgctc cttcaagact tgtggaatgc aaccaatttt tccgtcctat
      301 caccagctct ttgatatttc gctccgcacg attatttgtg agttctaaac gaccatcttc
      361 aaaaataggt tcaaaaccac ttcgtagttt acacgcatat tgaacggctg catccaactt
      421 ggattttgca acaggatcta tcgttgccat ccactcataa aaagcaataa ataatggacg
      481 cagttcttgt tgccgacagc ttaatcgctt ctcaggagaa aagtctttcc atttcttttc
      541 taggacaaac catttatcca attgtttgac tgcttgagct gcgggaatat ccgtgttttt
      601 ctttcctttt ggaattgctt cgaaaaattt ccgccgaaca tgcgcgaaac aatagacaag
      661 tcgtacactt tccacggcat tatacccgct atagccgtca cagtgtaaat accctgtaaa
      721 ctccttgaga aaagttttcg gaacatctcc agcacgactt tcgtcgtggt gataataaac
      781 gatgggatgc tgactgtctt ttcctgtaca aaactgccag taataggtat ctgttttggc
      841 actttcaatc acacgataac ttgtttcatc cccgtgtaga atctcttctt gattaagata
      901 gactcttaat cgttctccta aagggtctaa cgcgttttgc actgctttaa tatgccaatt
      961 ggtgatatta tctctcgaaa tatccaaccc atgtgtttgt ttccaatagt tctcttgccg
     1021 ataggcagga actttctgac caaatttcat ccggatcgtt tccgccacta aactattgga
     1081 acctaagcta ttagaaatca aaggtttagg aacgatcgct tttttaaaag aagaaagtcc
     1141 ttctttttcg cagcttttac aacaataggt atgttgataa tgggtgagac aatccaacat
     1201 agcctgatgg aaacgaattt cttctcgtac ttttgtccgt ccgatatctt tcatctgtga
     1261 accgcaatgt tcacaaaatt gttcttccaa aggaaggaga tgttctttct ctgttatagg
     1321 taagtgtttg attttgtctg ctttcgttcc tacaactcgt tgatgtcctt ttactggaat
     1381 aatggtggca gctgcttctg gaacacttac agaaatttct tcatcactaa aaagattcaa
     1441 ttgcccattc gtttcctcaa agagagtctc tttagagcgt ccaaataatt tgttggttaa
     1501 ataattgacc ttttcattca atagttggat ttcagttgta agattctgaa tggtttgttg
     1561 ttgtgcttga ttggttttat gggcttcttg aagcaactga agcaacgtat ctgtagtttc
     1621 caaaattttt caccaccttg aatcgaactg attttgttac agaataccac aagaaacgca
     1681 caagaaatag gggttcaaca cattttttgt gttaaccccc cagcttaaaa aactcctttt
     1741 tttcctggca aaatagctcg aggttgatcg atacttaatc cttctaacaa ccatcgaacc
     1801 tgttggttcg ttaaggttcg aacttcgtct ttcgtccgag gccattttaa cttgccgttt
     1861 tctatccgct tataacagag aagaaaacca tcaccgtccc aatagagaag tttgaaacgg
     1921 tcttgacggt tcccgcaaaa taaaaaaacg gcatcttcgt aaagctcgag ttgatactct
     1981 tgttgaataa gactggcaag accatcaatt ccttttcgca aatctgtttt tccgcacaca
     2041 atataaatat gtttggtttg aagtaaatca taaagcattg gacaacagtt ctttcaaaat
     2101 agcttgaaga atataattgt taataccgtt atagatcaaa acttcagaac cattctctct
     2161 tttgatgcgc atcgctaact tggcagccgc gggtttacct ttttctggtg cactcttttc
     2221 gtttcctaat tggacaggaa caatctcatt taatttatac atagaaaacc tccagacttc
     2281 ttgatagctc aagtatatct tgaggtgcat ttgttagtaa ggtactgttc tatggggcgc
     2341 tta
//
LOCUS       DACEOE010000256         2307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.265, whole genome shotgun sequence.
ACCESSION   DACEOE010000256 DACEOE010000000
VERSION     DACEOE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.265"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..1147
                     /locus_tag="IW774_002680"
     CDS             2..1147
                     /locus_tag="IW774_002680"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ1155000.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1318..1776
                     /locus_tag="IW774_002681"
     CDS             1318..1776
                     /locus_tag="IW774_002681"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1155001.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1935..>2307
                     /locus_tag="IW774_002682"
     CDS             1935..>2307
                     /locus_tag="IW774_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1155002.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNF"
BASE COUNT          766 a          434 c          440 g          667 t
ORIGIN      
        1 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
       61 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      121 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      181 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      241 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      301 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      361 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      421 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
      481 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
      541 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
      601 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
      661 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
      721 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
      781 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
      841 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
      901 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
      961 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1021 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1081 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1141 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1201 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1261 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1321 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1381 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1441 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     1501 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     1561 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     1621 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     1681 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     1741 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     1801 aagcaatata gctatcttat cttcgtattt tattaaatat ttattgtctg aaaagagttc
     1861 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
     1921 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
     1981 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
     2041 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
     2101 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
     2161 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
     2221 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
     2281 tcactaaagc aataaaatgg aatttta
//
LOCUS       DACEOE010000257         2295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.266, whole genome shotgun sequence.
ACCESSION   DACEOE010000257 DACEOE010000000
VERSION     DACEOE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.266"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            206..2068
                     /locus_tag="IW774_002683"
     CDS             206..2068
                     /locus_tag="IW774_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1155003.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2295
                     /locus_tag="IW774_002684"
     CDS             2068..>2295
                     /locus_tag="IW774_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAQ1155004.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSK"
BASE COUNT          824 a          342 c          449 g          680 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaa
//
LOCUS       DACEOE010000258         2216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.269, whole genome shotgun sequence.
ACCESSION   DACEOE010000258 DACEOE010000000
VERSION     DACEOE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.269"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            203..1204
                     /gene="ccpA"
                     /locus_tag="IW774_002685"
     CDS             203..1204
                     /gene="ccpA"
                     /locus_tag="IW774_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1155005.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLADRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEGFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(1541..>2216)
                     /locus_tag="IW774_002686"
     CDS             complement(1541..>2216)
                     /locus_tag="IW774_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1155006.1"
                     /translation="YEEGYIIDSITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSV
                     INAKITTPFKDAISSLNGNLGKADWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDN
                     TGMDSKARIRSANYLANLINQAYQAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKV
                     TVNKKSIQTPGKTVESLWAKNGPKKSAFKFGVGGTDENYTDYWNTASAYARANQATKE
                     SDEKDD"
BASE COUNT          703 a          396 c          439 g          678 t
ORIGIN      
        1 cctactttct aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa
       61 cgttctaaaa actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat
      121 tcttgagatt atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata
      181 aaatatgaat gggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag
      241 agaggcaaat gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgttaa
      301 accagcaaca agaaaaaaag tcttggaagt catcgatcgc ttggattatc gtccaaacgc
      361 agttgcgcgt ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca tccctgatgt
      421 cagcaatatg ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa
      481 atacaatatt atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa
      541 taacttactg gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga
      601 aatccgtgga gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc
      661 agatgaacaa gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa
      721 taaattggca aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc
      781 tatcaatgga caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc
      841 atatagcgaa ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagc
      901 cgatcggatc cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat
      961 cggtattttg gatggtttat tagatcgtgg cgtgaaagta ccggaaggat tcgaaatcat
     1021 cacaagtaac aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca
     1081 accattatac gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga
     1141 agaagtcgaa gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa
     1201 ataataaaag gacaaccatt cagaggttgt gagcctgccg tttaactctg cgttacaagg
     1261 agcaatttca aaaaacagca tttcatgttt gatgaatatt gtgactcgtg attcggagtt
     1321 ggcaggagaa caccgtttat tcgggaaaag gggttatgac aaaattttgt tataatcctt
     1381 tttttgtgtc ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa
     1441 tccgtgtata gatgctgcat tttgttcacc accatcaaaa aaataaggga ctgtgacaat
     1501 actattgtca caatctctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg
     1561 attctttggt tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat
     1621 agttttcatc ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg
     1681 cccatagcga ttcaactgtt tttccaggtg tttggatgga ttttttattg acagtaactt
     1741 ttccaggtgt ttgaccagta aatccagata cttttttctt catcacatca gatgataatt
     1801 cgaatttctc atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat
     1861 tggcaagata attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt
     1921 gccctgccca gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt
     1981 attcattggt agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc
     2041 ttgagatggc atctttgaat ggtgtagtga tttttgcatt gatcacactg cgcatcatgt
     2101 cattcatgat cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat
     2161 aaattacatt tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatag
//
LOCUS       DACEOE010000259         2209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.270, whole genome shotgun sequence.
ACCESSION   DACEOE010000259 DACEOE010000000
VERSION     DACEOE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.270"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..633
                     /locus_tag="IW774_002687"
     CDS             <1..633
                     /locus_tag="IW774_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1155007.1"
                     /translation="QKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPE
                     IPHKVFKDIQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVG
                     RKIRSIRKKLGLTMQEFGYEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDL
                     LFDEGFISEKSQVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            673..1284
                     /locus_tag="IW774_002688"
     CDS             673..1284
                     /locus_tag="IW774_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1155008.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
BASE COUNT          884 a          254 c          423 g          648 t
ORIGIN      
        1 caaaagctga aattattatc aactatcact aattcaacaa ttgactggat taaatgggga
       61 actttagaag aatatataac ttcatacctt atcgatatag ggtatgagct atatattaag
      121 gactttccag aaatacctca caaagtcttt aaagacattc aagaaagata tagtaatacg
      181 ttttcgctaa ataaagacta tgagttattg aatcctataa taaaaaatat atttacaaaa
      241 tattactcaa aagattttga agattatcga gacatagaag ttaaaccaga ccctaaaaag
      301 gtagggagaa aaattcgttc tattagaaaa aaattaggac ttactatgca ggaatttggg
      361 tacgaggttt ctaattctcc tagaagcact gtgtcaacat gggaacatgg tggtaactta
      421 ccgaacaaag ccaaactaaa aaaaattgca gacattgcga attgtagtgt agaagatttg
      481 ctttttgatg aaggctttat ttctgaaaaa tcccaagtta aattgataaa tgaactcaaa
      541 gaagaaaatt caaagctaaa agaaaaaata aaatactatg aatgtttatt tgatggtata
      601 gagagtctgc tgaactcacg aaaaaattcc tagttaatta aactagagta aaattatttg
      661 taggtgttaa atatgttagt tggctatgca cgagtcagtt cagatgatca aaatttggaa
      721 agacagcttg aagaatttag aaaaattggt gtcgaaaaag tatttgctga gaaaaaatct
      781 ggacgtaaca cagtagaacg gccagagttc aataaaatgt tgtcatttgt cagagaaggg
      841 gatacgcttg tttttgagtc gcttgaacgc ttaggaagaa attctgatga aatactagaa
      901 acagtatctt ttatagataa aaatggtatg ggcttaatgg ttttgaattt accaatttta
      961 gatacaaaat ttggtgatcc aaacttagaa aaacttgtta gaagcttagt aattaacatt
     1021 ttttcttgga cggctcaaaa tgagagggag gaaattaaga gaaaacaaaa acaaggaata
     1081 gcaattgcga agaagaaagg agtctataaa ggaaaaccct acgaatattc ttctactgca
     1141 aagaatccac aaaagaggtt tatctataat gaaatagtaa gaatgcttgg agaaggtgtt
     1201 ccgattaagc gaattgcaga aaaaacagga acaaatagaa atttagtcta tagaataaag
     1261 aaaagaataa gtgaatctga ttgattttaa gttctttgag aaatcaatgt tacgagagaa
     1321 ggattctaag cctgtttcaa acttttaagg tgtacaatta tacaaagaag cttctaaaca
     1381 aaagaattaa ttttgtggca gtcaagtaac gacagaaata gaaaagaaag aggctatgcg
     1441 agagtgaatc aacaagttaa ttgggttaat gattttgaaa aagaatttcg actgaaattt
     1501 tttggaaatc ctacagcaga aagtacagaa gaaaaaaatt atgactggtc agaattttag
     1561 gttgcatatt cgattcaata agtgttgaag ataatagttc tcttacgcca cagtatacaa
     1621 cgttagaata tgacgaaaac agattcaata catttccttg caataccatt caagaatcaa
     1681 ttgataagca tgagtgataa ttgattatct acaggaaggt gttttttatg aaactagaga
     1741 tcaaagaagt tgtctgcgat tggggaattt atgtagacgg agaaacatat ccatttatga
     1801 tttttaatag taaagcaaat gcacaagaga taatgcgtat catggagttg gataataagc
     1861 acgaaagatt tgattaagta aaaaaatgga gaatgaatgt gaagcagtgg aataccaaaa
     1921 aacatgcgcc aataataagt atattttagg tgcaatggga ttaggtaaat cagcttctat
     1981 taaacaggtc tacgaagaat cacaggagaa ttgcgtatat gaaaattcga attaaagagt
     2041 gagaatatgt gggagctaat ttatttagtt ttatacttaa taggaaaatc aactgaattt
     2101 gtatataaca ataaggtaat atttatattg ttcttaatag tttgtattat cgtatttgat
     2161 gtctatgatt tactaattca taagaatgaa aatagtaata ttggtagaa
//
LOCUS       DACEOE010000260         2207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.271, whole genome shotgun sequence.
ACCESSION   DACEOE010000260 DACEOE010000000
VERSION     DACEOE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.271"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            88..984
                     /locus_tag="IW774_002689"
     CDS             88..984
                     /locus_tag="IW774_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1155009.1"
                     /translation="MEINLFEAGNRIKQIREKHGYTMADLGKLVDANSPSTINNWEKG
                     NNLPNRKRLEKIALLGGTSVEWIKYGDFSDYVYRLTQNIEEKILKEYGETTLNNYREA
                     LLDELTIEKITYEEDTKILAAAEKINTQNIFQEVKYMYSPDEDYRNIEVKPDLGLTDD
                     NKNIKMIGQRIRKIRKKENITLEEFGKLFSPTADKAVISNWENGKNLPNSERIKKIAE
                     IGGVSELYLMTGVDSSVAESMYFLSEVALDALERLSIEEVNRIIESFAGYLNVISKID
                     DPDKREISLNAICQLSLLDKMG"
     gene            1033..>2207
                     /locus_tag="IW774_002690"
     CDS             1033..>2207
                     /locus_tag="IW774_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1155010.1"
                     /translation="MKPNLSLTSERIRKIIESKGLTLERFGDLIGDTPISSVNNWLRG
                     VSLPNKNRLLTIANIGNTSVDWIKWGTLEEYIASYLIDSGYELYIKDFPEIPYKVFKD
                     IQEKYSDTFSLDKDYELLNPIIKFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTT
                     LTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNVELKNLNIGNNIKNIRKSKN
                     MTQTDFAELMGLSRSYIGDLENNRSNPSIKTLEAIARALDVPIVALINDSSTDNIVEP
                     YESLTAENILLKQENEELKYEVESLRKRLYEIQQVVNQGAEKMIPNLKDVSKRIKYIR
                     KKLGMTMEQFGSHLSNSPKSTIATWESGRNIPSQKKLQLIAIVGETTTDWIKWGTLE"
BASE COUNT          857 a          314 c          410 g          626 t
ORIGIN      
        1 cctagttttt atgaatgacc gttttgtaaa tctgattgaa atagatttat atgatttcgg
       61 tatatgatat acattaggag gtgtcgaatg gaaataaatc tttttgaggc tggtaatcga
      121 ataaagcaaa tacgagaaaa acatggatat actatggcag acttaggtaa actggttgat
      181 gccaatagtc ctagcacaat taacaattgg gaaaagggaa ataacttacc taatagaaag
      241 cgtcttgaaa aaattgcact attaggtggt acttctgttg aatggataaa gtatggagat
      301 ttttccgatt atgtttatcg cttgacacaa aatatagagg aaaaaattct taaagagtac
      361 ggagagacta cactgaacaa ctaccgtgaa gctcttctag atgaacttac gattgaaaaa
      421 atcacatacg aagaagatac aaagatatta gcagctgccg aaaaaatcaa tacacaaaat
      481 atttttcagg aggtgaagta catgtattct cctgatgaag attatcgaaa tatagaagtc
      541 aaaccagatt taggactgac tgatgacaac aaaaatatta aaatgattgg acaacgtata
      601 aggaaaataa gaaaaaaaga gaatattaca ttagaagaat tcggcaaact attctctcct
      661 acagctgata aagctgttat aagcaactgg gaaaatggta aaaaccttcc taactcagaa
      721 agaattaaaa agattgcaga aattggcgga gtttctgaac tttatcttat gacgggtgtc
      781 gattcaagcg tagcagaatc aatgtatttt ctatctgaag tagcattgga tgctttggaa
      841 cgtctgtcaa ttgaggaagt caatagaata attgaaagct tcgccggtta tcttaatgtg
      901 atttcaaaaa ttgatgatcc agataagcga gaaatatctc tcaatgctat ttgtcaacta
      961 tcacttctcg ataaaatggg ttgattcaca aaaaatattg aaagattact ttactcacga
     1021 ggaggcttag ctatgaaacc taatctatcg ttaactagtg aacgtattag aaaaattatc
     1081 gaatccaaag gtctgacatt agaacgattc ggtgatttaa ttggcgacac accaataagt
     1141 tctgttaata attggcttag aggcgtgtct ttacccaata aaaataggct tttaacaatt
     1201 gcaaatattg gaaacactag tgtagactgg attaaatggg gaactttgga agaatacata
     1261 gcttcatacc ttatagattc aggttatgag ctatatatta aagactttcc agagatacct
     1321 tacaaagtct ttaaagacat tcaagaaaaa tatagtgata cgttttcact agataaagac
     1381 tatgagttat tgaatcctat aataaaattt actatagggg gcgttacttt gaatagaata
     1441 aaattttata ttgattccag taaaaagacc attaaacaaa ttagtacaga aactggtatt
     1501 aactatacaa ctcttacgaa ctattaccgt ggtgagcgaa atcctagaaa tactgaaaca
     1561 tggaatatcc ttgctgatta tttccatgtt cctgtaagtt atttgatggg attagacgaa
     1621 acaaatgttg aactcaaaaa ccttaatatt ggaaacaaca tcaaaaatat tagaaaaagt
     1681 aaaaatatga cacaaactga ttttgctgag ttaatgggtc tatcacgatc atatataggg
     1741 gatttagaaa acaacaggag taatcccagt ataaaaacac tggaagctat tgcaagagca
     1801 ttggacgttc cgatagtagc attaatcaac gattcttcta ctgataatat agtagagccg
     1861 tatgaaagtt tgacagccga aaatatcctt cttaaacaag aaaatgagga attaaaatat
     1921 gaggtagaaa gcttacgaaa aagattgtat gaaatccaac aagtagtaaa tcaaggagct
     1981 gaaaaaatga ttcccaatct aaaagacgta agtaaaagaa tcaaatatat cagaaaaaaa
     2041 ttaggtatga ctatggagca atttggttct catttaagca actcacctaa aagtacaatt
     2101 gctacttggg aaagtggtag aaatatacct tctcagaaaa agttacagct gattgctata
     2161 gtaggagaaa ccaccactga ctggattaaa tggggaactt tagaaga
//
LOCUS       DACEOE010000261         2197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.272, whole genome shotgun sequence.
ACCESSION   DACEOE010000261 DACEOE010000000
VERSION     DACEOE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.272"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..1468
                     /locus_tag="IW774_002691"
     CDS             5..1468
                     /locus_tag="IW774_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321529.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1155011.1"
                     /translation="MTGLVTLAFGHVAQADNTTTAESSTSETMQKIEPKKDVYVIASD
                     SAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAALQALESGQADGMIA
                     GMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTVGAKVGTESAEFLS
                     DHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQGQELETPIEKESGG
                     KYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSETSSESSTADESTFL
                     GLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRALRTIASIYVDIIR
                     GIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIVRGGINAVPVGQME
                     ASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISAIGVVELLQTGKII
                     VARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            1470..>2197
                     /locus_tag="IW774_002692"
     CDS             1470..>2197
                     /locus_tag="IW774_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1155012.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKV"
BASE COUNT          714 a          404 c          467 g          612 t
ORIGIN      
        1 cttaatgaca ggacttgtga cacttgcgtt tggtcatgtt gcacaagctg acaacactac
       61 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
      121 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
      181 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
      241 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
      301 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
      361 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
      421 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
      481 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
      541 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
      601 ttatgcgatc aaacaaggac aagaattgga aacaccgatc gaaaaagaat ctggcggaaa
      661 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
      721 attgaaagaa ttaaaaagaa caggcgaata cgataagatt atcaaagaat atgtagcgga
      781 tggttcagaa acttctagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
      841 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
      901 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
      961 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
     1021 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
     1081 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
     1141 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
     1201 ttcttataat cggacaatgc aaaaaatcat tttgccacaa gcaatacgca tcatgatccc
     1261 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
     1321 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
     1381 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
     1441 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
     1501 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
     1561 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacatttttg cgttgcttga
     1621 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
     1681 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccag cactttaacc
     1741 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
     1801 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
     1861 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
     1921 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctaca tctgcactcg
     1981 accccgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
     2041 cgatggttat cgttacgcac gaaatgggct tcgctaaaga agtggccaat cgtgtcatgt
     2101 tcattgacga cggaaacttt ttagaagacg gaacgcctca acaaatcttt gaaaatccgc
     2161 aaaatgaacg gacaaaagat ttcttgaata aagtatt
//
LOCUS       DACEOE010000262         2191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.273, whole genome shotgun sequence.
ACCESSION   DACEOE010000262 DACEOE010000000
VERSION     DACEOE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.273"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..531
                     /locus_tag="IW774_002693"
     CDS             <1..531
                     /locus_tag="IW774_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1155013.1"
                     /translation="NGVRYKSLDQIEALLSVSVPRELFDITQFNIIHGDLCFANIMVD
                     NTFSFIKVIDPRGKFGDFDIYGDYRYELAKLFHSVDGKYDFIIKDLFTIKYDPKKAII
                     DYIVQDRKRDYDLYEVFYSVFKDEIGSDLKKIELIEALLFLSMIPLHGESLNHQMAML
                     ATGLEILGRVVPDIYC"
     gene            619..1017
                     /locus_tag="IW774_002694"
     CDS             619..1017
                     /locus_tag="IW774_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1155014.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            1042..1758
                     /locus_tag="IW774_002695"
     CDS             1042..1758
                     /locus_tag="IW774_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155015.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            1913..>2191
                     /locus_tag="IW774_002696"
     CDS             1913..>2191
                     /locus_tag="IW774_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAQ1155016.1"
                     /translation="MVAGLSTVAVPTFFIISSFLLCSGEVTSAKLSKQIYRVLKIYII
                     WSILYIPLVLKNWVQSDFSWKTDVLLYIRDFLFIGSYYHLWFLPALAFA"
BASE COUNT          691 a          286 c          461 g          753 t
ORIGIN      
        1 aatggagtca gatataaatc tttggaccag atagaagctt tattgagcgt aagtgttcca
       61 agagaattgt ttgacattac gcaatttaat attattcatg gtgacttatg ctttgccaat
      121 attatggtag ataacacatt ttcgttcatt aaagtcatag atccgcgagg taagtttgga
      181 gattttgata tttatggtga ttatagatat gaactggcaa aattgtttca tagtgttgat
      241 ggaaaatatg actttataat taaggacttg tttacaataa aatatgatcc aaagaaggca
      301 atcatagact atattgttca agatcgtaaa cgagattatg atttatatga agtattttac
      361 tctgtcttca aagatgaaat aggttctgat ttgaaaaaaa ttgaacttat tgaggctttg
      421 ctgtttttaa gtatgattcc tttgcatgga gaaagcttaa accatcagat ggctatgtta
      481 gcaactggtt tggaaatttt aggacgagtt gtcccggata tttattgtta atgctttgag
      541 ctattgtttt tctatttgat cagtgtaaag ccgggcatat aaattgcctg gctaaaataa
      601 aggagatgtc ttagttatat gtttaaagat tacgcatttg tttttgatat tgatggaact
      661 ttgtgcccca tcaaaaagaa agatgagaaa tatgaagatt tagttccgta tgctaatatg
      721 gtggagaagt tgcgatacta taaggagaac ggagcaaaga ttacactgtt cacatctcgc
      781 aatatgaatt cttatcatgg taatttaggt gtgatcaatg caaatactgc aaaaatcttg
      841 ttggcttggc tagataagtg ggagattccg tatgatgaaa ttctttatgg aaagccttgg
      901 ccaggccata aagggtttta tgttgatgat cgcacagttc gtccggatga atttttatcg
      961 tgtaccctgg atcagctcga tgagatttgc aaaaaatcca aattgaaatc tgagtaaagg
     1021 agactttata aagtgaataa gatgacggtt attattacaa tggctggtct tggcagtcgc
     1081 tttcgtaaag ttggctacac tgtgcccaaa tttatgattg aagcaaaagg taaaacctta
     1141 tttgattggt ccttagacag cttgcttgat tataattcac atgtagaaaa gtatgttttt
     1201 gttgtgcgta aggaagatga tgctactgtt tttatcaaag aacattgcag tctgtatgga
     1261 attgaaaatg tcgaggtagt tgaaattgac tatatgactg atggacaagc cactacatgt
     1321 atgcttgcaa ttccttattg cgcacctgat aatgcaatta tggtttataa catagataca
     1381 tatgttgagc caggtgagat gaaatacgtt gatatttctg gagaaggaca tattccgtgt
     1441 ttccatgcgg acggtgatca ttggagtttt gccaagttga acgaatatgg tgatgtagta
     1501 gaagtaagag aaaaagttag aatttctaac aattgtactt taggtgcata ttatttttct
     1561 tctgcaaaat tatatgcatc actctacgaa gaatattatg cagatgaaag gcatctggaa
     1621 aagaatgaga aatatattgc gccgctatat aattatatga tagagaaggg catgaaggta
     1681 accattagta tagtagataa atcaaaagtt cacgtactcg gtactccaga agagctcgat
     1741 gtgttcttaa atgaataggg aaaatataat catagaaaat gttaattata aagctgcgtg
     1801 tgtgggggag tactattcga tagatattct aaaaatacta atggctattt tagttgttgt
     1861 tcgtcattta ggtcaaaact atttagctcc agattaagca tttagtattg gtattgttgc
     1921 aggactgagt acggttgccg ttcctacttt tttcatcatc agttcctttt tgttgtgtag
     1981 tggtgaagtt acatccgcta aattatctaa acaaatctac agagtactta aaatttacat
     2041 tatttggtct atattatata ttccattagt attaaagaat tgggtacagt cagattttag
     2101 ctggaaaaca gatgtattat tgtatatcag agattttctg tttattggta gttactatca
     2161 cctgtggttc ttgcctgcac tggcttttgc t
//
LOCUS       DACEOE010000263         2174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.274, whole genome shotgun sequence.
ACCESSION   DACEOE010000263 DACEOE010000000
VERSION     DACEOE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.274"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            188..511
                     /locus_tag="IW774_002697"
     CDS             188..511
                     /locus_tag="IW774_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155017.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            675..929
                     /locus_tag="IW774_002698"
     CDS             675..929
                     /locus_tag="IW774_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155018.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            999..1244
                     /locus_tag="IW774_002699"
     CDS             999..1244
                     /locus_tag="IW774_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1155019.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1341..1685
                     /locus_tag="IW774_002700"
     CDS             1341..1685
                     /locus_tag="IW774_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155020.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
BASE COUNT          613 a          374 c          363 g          824 t
ORIGIN      
        1 catcataaat aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca
       61 caagtttcaa aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata
      121 actattttgt tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt
      181 attctcaatg ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc
      241 ggcagcagct tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat
      301 gatttttcaa ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa
      361 aagatatgcc cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat
      421 catcattctt tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt
      481 agatatcaat ccattgattt tttctcgata gataagacac attcagcgga acagttcctg
      541 cttatcagca gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt
      601 tcctagcatt tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt
      661 gaggagtccg ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac
      721 tggtcgcaag tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt
      781 tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg
      841 atagtgtttt tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt
      901 atttgatacg tttaaataaa aaacattgag tactattgat tgcatgtgta gctttctata
      961 gtcattataa ctattctact ataatgaaag gtgattaaat gagtatcgaa gaagctattt
     1021 tactggatac tttacgttgt cgtctgaatt tcttaagaac tggccgatct ttatctcaag
     1081 tagctgtttc tagcgcgtta agtattcctt tattcaaata tcgtaagtac gaagaaggag
     1141 aagaaattcc aagcatcatt gaattaatct atatcgctga cttttacgat atttccgttg
     1201 attacctaat tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt ccagccttgg
     1261 tgctggtttt ttcatttaac gtctaaggtt aacaatctat atataaagga gacatttcta
     1321 tgtgattatg gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt ttaggacaac
     1381 atcgggaatg ctgtgcatta agaggaaatg gctggggaaa gaaacatgct actgtggatt
     1441 atgtttttca ctattctcct tatagattat tccaatatca tcagcttgtc atggatgaaa
     1501 tggaaaaacg tggttacagc ccagcaccag agtggaaaaa tcctatgtat cgtggcaaat
     1561 catgcgaagc attttttgat ttacctactg tagagcgcaa cgtgccgatt tatccggaac
     1621 acgatgaagt ttatctagca gaatgtttag caaatttgga gcaaaaagga atttatctta
     1681 cctaatataa ctagaaaagc cttctatctc ctcttacttc attaaaaagc tttaaccatc
     1741 ttagatcaag ctaaaatgtc gtaagtttta gggttatctt catcctcacg tgcaaaacta
     1801 aaagcgtact ctagttttgg ttcatcgcca acttccctag acataaatga acatctcctg
     1861 acgtggttta aaagtaccgc atcttcgacg tctgtatacg atgcacagca cagattgctt
     1921 cttttgatct ttcttttgat ttttctgttg cttcttctac accttcttcg attactacat
     1981 cgatgcttac aattgcttct gcctaatatt cccatgcatc gtcgatttaa gcctctgtag
     2041 tcactgtgat cgtttccaca ttttaactcc tctgtcgtgg cttcagttga ttttgtgatc
     2101 agttcagttg ctaagccaga gtcagaacaa cctgccattg ctagcgttaa tattgatagt
     2161 aaaaatagtt tttt
//
LOCUS       DACEOE010000264         2167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.275, whole genome shotgun sequence.
ACCESSION   DACEOE010000264 DACEOE010000000
VERSION     DACEOE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.275"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..111
                     /locus_tag="IW774_002701"
     CDS             <1..111
                     /locus_tag="IW774_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1155021.1"
                     /translation="YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            325..1020
                     /gene="vanR"
                     /locus_tag="IW774_002702"
     CDS             325..1020
                     /gene="vanR"
                     /locus_tag="IW774_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1155022.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            998..2152
                     /locus_tag="IW774_002703"
     CDS             998..2152
                     /locus_tag="IW774_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1155023.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          731 a          400 c          461 g          575 t
ORIGIN      
        1 tatgcggtaa agctatataa agaaggaaat atgactgtaa atcaaatttg tgaaattact
       61 aatgtatcta gggcttcatt atacaggaaa ttatcagaag tgaataatta gccattctgt
      121 attccgctaa tgggcaatat ttttaaagaa gaaaaggaaa ctataaaata ttaacagcct
      181 cctagcgatg ccgaaaagcc ctttgataaa aaaagaatca tcatcttaag aaattcttag
      241 tcatttatta tgtaaatgct tataaattcg gccctataat ctgataaatt attaagggca
      301 aacttatgtg aaagggtgat aactatgagc gataaaatac ttattgtgga tgatgaacat
      361 gaaattgccg atttggttga attatactta aaaaacgaga attatacggt tttcaaatac
      421 tataccgcca aagaagcatt ggaatgtata gacaagtctg agattgacct tgccatattg
      481 gacatcatgc ttcccggcac aagcggcctt actatctgtc aaaaaataag ggacaagcac
      541 acctatccga ttatcatgct gaccgggaaa gatacagagg tagataaaat tacagggtta
      601 acaatcggcg cggatgatta tataacgaag ccctttcgcc cactggagtt aattgctcgg
      661 gtaaaggccc agttgcgccg atacaaaaaa ttcagtggag taaaggagca gaacgaaaat
      721 gttatcgtcc actccggcct tgtcattaat gttaacaccc atgagtgtta tctgaacgag
      781 aagcagttat cccttactcc caccgagttt tcaatactgc gaatcctctg tgaaaacaag
      841 gggaatgtgg ttagctccga gctgctattt catgagatat ggggcgacga atatttcagc
      901 aagagcaaca acaccatcac cgtgcatatc cggcatttgc gcgaaaaaat gaacgacacc
      961 attgataatc cgaaatatat aaaaacggta tggggggttg gttataaaat tgaaaaataa
     1021 aaaaaacgac tattccaaac tagaacgaaa actttacatg tatatcgttg caattgttgt
     1081 ggtagcaatt gtattcgtgt tgtatattcg ttcaatgatc cgagggaaac ttggggattg
     1141 gatcttaagt attttggaaa acaaatatga cttaaatcac ctggacgcga tgaaattata
     1201 tcaatattcc atacggaaca atatagatat ctttatttat gtggcgattg tcattagtat
     1261 tcttattcta tgtcgcgtca tgctttcaaa attcgcaaaa tactttgacg agataaatac
     1321 cggcattgat gtacttattc agaacgaaga taaacaaatt gagctttctg cggaaatgga
     1381 tgttatggaa caaaagctca acacattaaa acggactctg gaaaagcgag agcaggatgc
     1441 aaagctggcc gaacaaagaa aaaatgacgt tgttatgtac ttggcgcacg atattaaaac
     1501 gccccttaca tccattatcg gttatttgag cctgcttgac gaggctccag acatgccggt
     1561 agatcaaaag gcaaagtatg tgcatatcac gttggacaaa gcgtatcgac tcgaacagct
     1621 aatcgacgag ttttttgaga ttacacggta taacctacaa acgataacgc taacaaaaac
     1681 gcacatagac ctatactata tgctggtgca gatgaccgat gaattttatc ctcagctttc
     1741 cgcacatgga aaacaggcgg ttattcacgc ccccgaggat ctgaccgtgt ccggcgaccc
     1801 tgataaactc gcgagagtct ttaacaacat tttgaaaaac gccgctgcat acagtgagga
     1861 taacagcatc attgacatta ccgcgggcct ctccggggat gtggtgtcaa tcgaattcaa
     1921 gaacactgga agcatcccaa aagataagct agctgccata tttgaaaagt tctataggct
     1981 ggacaatgct cgttcttccg atacgggtgg cgcgggactt ggattggcga ttgcaaaaga
     2041 aattattgtt cagcatggag ggcagattta cgcggaaagc aatgataact atacgacgtt
     2101 tagggtagag cttccagcga tgccagactt ggttgataaa aggaggtcct aagagatgta
     2161 tataatt
//
LOCUS       DACEOE010000265         2161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.276, whole genome shotgun sequence.
ACCESSION   DACEOE010000265 DACEOE010000000
VERSION     DACEOE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.276"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..184)
                     /locus_tag="IW774_002704"
                     /pseudo
     CDS             complement(1..184)
                     /locus_tag="IW774_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            263..439
                     /locus_tag="IW774_002705"
                     /pseudo
     CDS             263..439
                     /locus_tag="IW774_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(615..1184)
                     /locus_tag="IW774_002706"
     CDS             complement(615..1184)
                     /locus_tag="IW774_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1155024.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1258..1581)
                     /locus_tag="IW774_002707"
     CDS             complement(1258..1581)
                     /locus_tag="IW774_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155025.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(1735..>2161)
                     /locus_tag="IW774_002708"
     CDS             complement(1735..>2161)
                     /locus_tag="IW774_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155026.1"
                     /translation="QIFGSERKVRYLLFCFLKDFPHFFADDGEQSFSEMFIQLLKQRK
                     DPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSCNQITEKRWKRIEE
                     TQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          584 a          440 c          380 g          757 t
ORIGIN      
        1 cgccccaaat attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag
       61 tgtctaatgt taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata
      121 ctttatagtt cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag
      181 gacttttggc tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa
      241 aacaccaaca ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga
      301 agatatcaaa ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat
      361 ttctcccgaa gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat
      421 aaaaaagcac tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt
      481 ttatttataa gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat
      541 cttaaaggcg ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg
      601 cgaacatggt tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc
      661 aattgagatt gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt
      721 tttcttctta actggtagtt gattttccca gtttccatca actcgctaag catatccacg
      781 actccgccaa atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga
      841 ttttttgctg taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct
      901 tccatccctt cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt
      961 tcttttggtt ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag
     1021 agcgtttcgt attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata
     1081 tatacgccag ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact
     1141 tctgtgatcg aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta
     1201 ctaatcgctt tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta
     1261 tcggtttttg cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg
     1321 atggtacaga tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat
     1381 cactagatct gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt
     1441 tcgatgtctt agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata
     1501 gcgaaaaaaa aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg
     1561 gagtgttttc agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat
     1621 cgctagccga aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg
     1681 ataataacga acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc
     1741 ttggtaaagt acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca
     1801 aaacagcgaa tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc
     1861 tatccgtttc caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata
     1921 atttttttct tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc
     1981 aaagaatagt gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt
     2041 caataattgg atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg
     2101 aaaatctttc aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg
     2161 t
//
LOCUS       DACEOE010000266         2154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.277, whole genome shotgun sequence.
ACCESSION   DACEOE010000266 DACEOE010000000
VERSION     DACEOE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.277"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            317..1174
                     /locus_tag="IW774_002709"
     CDS             317..1174
                     /locus_tag="IW774_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1155027.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1167..2069
                     /locus_tag="IW774_002710"
     CDS             1167..2069
                     /locus_tag="IW774_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1155028.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
BASE COUNT          802 a          342 c          406 g          604 t
ORIGIN      
        1 tttttacata tctattttcc tgttacctta agattaaatg agaatttaaa aaaatgaaac
       61 taacttgctt tgtcattatg aattttttca aaaaacattc ttaagaaaaa ggtggctata
      121 tatagtagtt tttggtcaat acctttgaat aaaaagccat gaaaaacgaa tagttagcgc
      181 ttaccgatgc gttttgtgat aaaaaaattt taaaatattc aatgaaaaca aagcatatat
      241 tttgctttaa aaccaaatga ggaatattct agatgtagaa caggttagaa aaagattaga
      301 aaaaggagtg attttgatgg agaagagata cggcgtagtt cctgtggtaa ctgcttcaga
      361 cgaaaattat gctccttact tgagcgtgat gatcgcaact gcattagaaa attgcaataa
      421 aacaagacgc ataaaatttt atgtgattga cgatggtcta tcagaatata gtaaagaagg
      481 attggaagaa acggtaaata aatattcaag taatgcaagt atccaattcc tgacagtgga
      541 aaaagatatc tacgaagact tcctagtcag tgaccatatc acaactaccg cttacttaag
      601 aatttcttta ccaaacttat tagctaaaga ggattataag aaagtccttt acttagattc
      661 agatgtgtta gtactagacg atatcgtaaa actatatgat gagccgttaa atggaaaaac
      721 aatcggcgct attatcgatc caggccaagt gaaagctttg gaaagattag gaatcgattc
      781 cgatgacttg tacttcaatt caggagtgat ggtgattgac attgatcaat ggaataaaaa
      841 agaaattaca gaaaaaacta tccattacct cagtgaaaat ggcgatcgaa tcatctatca
      901 cgaccaagat gctttgaatg ctgtacttta tgaagattgg gaacaacttc atccaaaatg
      961 gaacatgcag acttcgctga tttttgaacg tcatccagca ccaaatgaaa aatatgaacg
     1021 tcagtataaa gaaggaaatg aaaaaccttc gatcgttcac tttacaggac atgataaacc
     1081 ttggaatacg ttgaaagatc atccgtatac caatttatac ttaaaaaaat tagcacacag
     1141 tactctaacg aaagtaggcg agatcaatga ataaaaaaga aatagcagta gtcgcaagtt
     1201 gtaacacaaa attcgtacca catttagctg cattatttgt ctcagtatta gataattgca
     1261 acccgtctaa atttgttcgg ttctatgtga ttgacgatga tatcgatttt gaaagtaaac
     1321 agcttttacg cttctcagtg aaaaacgcac gaatgaactc agatgtggaa ttcttgaaaa
     1381 tcaataaaga attctttaca aatgtggtaa tcagcgatcg tattccagaa acagcttatt
     1441 atcgtattgc gattccggaa ttgttccgcg gcacagaagt tgaacgtatc ttatatatgg
     1501 attgcgacat gatcgcttta caagatatct ctaaattatg gcgtcttgac ttcggtgatt
     1561 caatcgtagc agctgttgaa gacgctgggt tccatcaacg actggaaaaa atgaaaattt
     1621 cagctaaatc aatgcgttat ttcaactcag gtttgatgct gatcaatgtg aaaaaatggt
     1681 tagatgaaaa tattacgcaa aaagtattag atttcatcga acataatcca gaaaaattac
     1741 gattccatga ccaagatgca ttaaatgcta tcttacatga ccgttggtta ccacttcatc
     1801 cgcgttggaa tgcacaaggc tatatcatgg caaaagcaaa aaaacatcca acagcagcag
     1861 gtgaacgtga atacgaagaa actcgcaaca atccttacat tatccatttt tcaggacatg
     1921 tgaaaccgtg gagcaaggat tttgaagggc caacgaagaa atattacgaa aaatatgcag
     1981 ggatgacggc tttccgttgt gtagctaaat tccctaaata tccaaaatat gcaaaagttc
     2041 aaccaaaaac agatgtagtt gttaaataat agaagacaac aactgaagag ggggagtagg
     2101 aatggaaatt acaatgtcta aaaatgcagt agagacgtta attgaaaagg ttgg
//
LOCUS       DACEOE010000267         2151 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.278, whole genome shotgun sequence.
ACCESSION   DACEOE010000267 DACEOE010000000
VERSION     DACEOE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.278"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            46..>2151
                     /gene="acm"
                     /locus_tag="IW774_002711"
     CDS             46..>2151
                     /gene="acm"
                     /locus_tag="IW774_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1155029.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPFHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVII"
BASE COUNT          848 a          343 c          416 g          544 t
ORIGIN      
        1 atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga
       61 aatattattt tatcaatgtt atttattatt actaatataa catcactgat tccttttcat
      121 gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca
      181 acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa
      241 aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt
      301 gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg
      361 gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta
      421 gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat
      481 acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca
      541 gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat
      601 actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca
      661 gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt
      721 aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt
      781 ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata
      841 actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata
      901 gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat
      961 aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt
     1021 ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag
     1081 attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga
     1141 cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa
     1201 gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta
     1261 agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct
     1321 atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc
     1381 actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca
     1441 gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat
     1501 atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa
     1561 aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca
     1621 aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac
     1681 caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc
     1741 caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa
     1801 tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt
     1861 acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct
     1921 ataccaggta gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa
     1981 attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa
     2041 caaagaagca attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa
     2101 agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat t
//
LOCUS       DACEOE010000268         2139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.279, whole genome shotgun sequence.
ACCESSION   DACEOE010000268 DACEOE010000000
VERSION     DACEOE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.279"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..640
                     /locus_tag="IW774_002712"
     CDS             <1..640
                     /locus_tag="IW774_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1155030.1"
                     /translation="RPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVG
                     EVCGLTWDDVDFSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHK
                     KQQLENRMKYGKLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTH
                     ATLLLENGAKMKEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(858..2000)
                     /locus_tag="IW774_002713"
     CDS             complement(858..2000)
                     /locus_tag="IW774_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1155031.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGICLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2005..>2139)
                     /locus_tag="IW774_002714"
     CDS             complement(2005..>2139)
                     /locus_tag="IW774_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1155032.1"
                     /translation="YANKIFKECKELLVEKGYFFYRNSKIKRIPISILEEVLHISWEE
                     "
BASE COUNT          697 a          392 c          333 g          717 t
ORIGIN      
        1 acgaccaacg actaaaaaag atctaaaaat catttctctt gaggactttg atcatatgct
       61 agaaattact cctgaaggac atcctttcta tattcctttg aatattggat tttatacggg
      121 aatgcgcgtt ggcgaagttt gtggtctgac gtgggatgat gtcgattttt caaatggaac
      181 aattactgta gagaaacaaa tggtaaagaa tgacggcgca tgggtatatg gtacgccaaa
      241 gacaagcagt tccaatcgaa cgatttttat tggacaaacc ttgctagcaa ttctgaaaaa
      301 acataagaaa caacaattag aaaatcgaat gaagtatgga aagctctaca ttgattcaaa
      361 tgcagtatgt acgaaggaag acggtgagct agttacgcca agtgtagtga aatggaatac
      421 taggaggata tcgaatgcac tctccctctc ttttaacttc cattctctca gacatactca
      481 tgctacactt cttctcgaaa atggcgcaaa aatgaaagaa atctctgaac gattgggcca
      541 cagcagaatt tcaattacga tggatactta ctcgcatgta acagataaga tgagaaatga
      601 aacggtcgat atcatggaaa atcttagaaa gaattcttga tttttgccac cgaaaaaaac
      661 caccggtggc aaaccggtgg caaatccatg taaacatggt ttatttttta cactgttttc
      721 aaacaaaccc ttgctatttc ttcggttcgt cctcgtccat tttcgatacc gacagctagc
      781 ttataaccca aggtatatca acataaaaaa aaaaaaatag gctgtgacag cagcgtgaca
      841 gcaaaaaaga tggaaagtta aatatcaaac ctcgtaattt catttgcgag tgcttgattt
      901 gtttctggat ataagtgtcc atatttgtcc aaagtagttt taatagaggc atgacctaga
      961 cgcttagata ttgcatagat atttttatta tgttcaatta aaaaggcaac atgcgagtgt
     1021 cttaatccat gcatatgaat tggttttact cctgcaattt cagctaattt ttttattctg
     1081 cttaaaatag ttcttggaga tggcggtaat ccatcaaaag aaaaaaccaa ttctatattt
     1141 ttcccaattg attgttgagt ttttttccat gtttttaaat cttcaacggt ttgtctgtct
     1201 aacgaaatta tccgaatact tgatgtattt tttgtttcag caaattcata atcttgtctt
     1261 gtacggatgt aaattgactt atttacgcta catctgccat ttattaagtc aaagtctttc
     1321 cattgtaacg ctaagagctc ctcactcctt acacctgtaa caaaaataaa acgcatcaaa
     1381 cgtttataaa attcttcata aaaatccccg tgataaacac tattatatac tttcttaaat
     1441 tcatcaactg tccaaaattc tacttgagga cgctgagttt ttattttacc tatagtatct
     1501 acaggatttt cttcaataat ttcaagtaca attgcccttt taaaaactac tctcaactta
     1561 ttgaatatct gtttaatata atttggtgat aatggttgct caatgccctt ttttacaaca
     1621 tggattttcg ataaaaactg ttgaaattcc tgtatatgaa ttggtcgaat ctttacaacc
     1681 tgcatttctc caaaaaaatc caatgcacgt tgtaaaatat gatctgtttt catatatgtt
     1741 ttttctacag ttcccaactt ataccaagga aaaaaatact ctcgataaaa atcatgaaac
     1801 gttattcctt gcagacaaat tccttgtgaa tcagaaaaac tttgttttaa ctcttcgagt
     1861 ccccttctag cttctccttg agttttgaag cctcttctgg tcacttgaat tctttttcca
     1921 aagcgatctt gtcctaaagt tgcagaaaaa aaccaagtgc ctctttttct gtctttataa
     1981 acattcgcaa ttttcctcat tttactattc ctcccaagaa atatggagaa cttcttctaa
     2041 aattgaaata ggaattcttt ttatttttga attacgataa aaaaagtatc ctttctcaac
     2101 taaaagttct ttacattcct tgaagatttt attagcata
//
LOCUS       DACEOE010000269         2130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.280, whole genome shotgun sequence.
ACCESSION   DACEOE010000269 DACEOE010000000
VERSION     DACEOE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.280"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            37..1377
                     /locus_tag="IW774_002715"
     CDS             37..1377
                     /locus_tag="IW774_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1155033.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1595..2038
                     /locus_tag="IW774_002716"
     CDS             1595..2038
                     /locus_tag="IW774_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1155034.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT          589 a          360 c          458 g          723 t
ORIGIN      
        1 aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa attgaaaaaa
       61 caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg tgcaggtcta
      121 tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat tgcttttttg
      181 attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt
      241 ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc tcattctgct
      301 gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc tgaagctgtt
      361 gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt atttgttttt
      421 atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt cgcagaaaca
      481 gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat ttttgctact
      541 tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca gcaattgact
      601 gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt gcttgtagtt
      661 atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc agatgatcct
      721 aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc cttttacatc
      781 attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg cacgaatgtc
      841 agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat
      901 tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc atcttcaaga
      961 attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac
     1021 aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta tcttggtgta
     1081 gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc cctttcgtat
     1141 acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc actaagaaaa
     1201 ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg attgattttc
     1261 cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct gctttacctg
     1321 ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat
     1381 cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga aaggagttgt
     1441 gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc tcttggtaaa
     1501 cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca tgacatgata
     1561 gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt
     1621 gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg tctatgcgct
     1681 cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg gtgcagtcat
     1741 ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat
     1801 gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac gaagccaaac
     1861 tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta tcgctatcct
     1921 ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat
     1981 tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc
     2041 attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca agaaataaga
     2101 agaagctact tctggaacgc cgtgaataag
//
LOCUS       DACEOE010000270         1997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.281, whole genome shotgun sequence.
ACCESSION   DACEOE010000270 DACEOE010000000
VERSION     DACEOE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.281"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            48..695
                     /locus_tag="IW774_002717"
     CDS             48..695
                     /locus_tag="IW774_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1155035.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            779..1723
                     /locus_tag="IW774_002718"
     CDS             779..1723
                     /locus_tag="IW774_002718"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1155036.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
BASE COUNT          696 a          337 c          418 g          546 t
ORIGIN      
        1 ttatacttgt aatagaatac tattttataa caaaggggat cgaatcaatg aaaaaaactg
       61 ttatttatac tagtttattt gcagctttgt ttttagctgc ttgtcaatca aataccaatc
      121 aaggcacatc tgggaatacg tcttccagta gcgaacagcg ctcttcacag gtatcggact
      181 cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa aagttctagt gaagcaagta
      241 ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga attgttccaa gataaatacc
      301 cagatactgc aattactagt ttggaattag atagtgattg gggatcttac ttctacaaga
      361 tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa
      421 tggagacaga gaatccagaa actctagatc gagatgaaca aaatggtcaa aagaaaaaag
      481 aagatggtct ggatgttgat aaattgatca cgattgagga agcagcaaaa atcgccgtcg
      541 acaaagtagg aagcggtacg gcaacagatt gggatttgga taaagattta ggtactactt
      601 attgggaagt caaagtaaaa gacggcaatc aaagtacatc agtcaagatc aatagccaaa
      661 caggcgaagt actgtcaact gaacaagatg attaagaatt tcttcggaaa acaagagttg
      721 atttttcttt tagtcatgat acactagaca aaataacttg tttgaaggag cgatttactt
      781 gagtttagaa acgacctttg ccaatcatat ctttgccaat cctttgatga atgcatctgg
      841 tgtccactgc atgactaccc aggaattaga tgaactggct cattcagaag caggagcatt
      901 tatcactaag agctgtacga tcaatgaacg aaaaggaaat cccgaaccac gctattttga
      961 tgttccttta gggagtatca attctatggg acttccaaac ttaggttttt cttattatct
     1021 agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc
     1081 cattgccggg atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg
     1141 gtttaatggg atcactgaat taaatttatc ttgtcccaat gtgcctggaa aaccacaact
     1201 agcttacgat tttgaggcca cttatgaaac actgaaagaa gtattcagca tcttttctaa
     1261 gccgctagga atcaaattgc ctccttattt tgattttgct cattttgatc agatggctga
     1321 tatcttgaat cagtttccat tgacctatgt caatgctatc aacagtgtgg gcaatggttt
     1381 gtatatcgat acagaacaag aagcagtagt catcaaacca aaagaagggt tcggcggcat
     1441 tggcggagag tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt
     1501 gaaaccagaa atccaaatca ttggtacagg tgggattcgg acaggacaag atgcatttga
     1561 acatctgcta tgtggcgcaa gcatgttaca gattggtacc gagcttcata aagaagggcc
     1621 ggaaattttt tctagaatca taaaagaatt aacgcagatt atgtcagaaa aaggatatac
     1681 gtcaattgac gaatttaaag gcaaattacg caccatttca tagcaaagag acgaaggtca
     1741 tgattgatct tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta
     1801 ctcattgtat gttagacgaa cttgtgggaa aataacagag gcaaaaagga ggataatatg
     1861 aatgatgcaa cagaattagc aaaagagctt aaagaaaaag aatttagttt tcaagaacta
     1921 gttacggata tacagaaaaa aataaagaag caaaatcatg aattgaatgc gtttgtcgcg
     1981 cttgaaccat tcgagag
//
LOCUS       DACEOE010000271         1957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.282, whole genome shotgun sequence.
ACCESSION   DACEOE010000271 DACEOE010000000
VERSION     DACEOE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.282"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(96..662)
                     /locus_tag="IW774_002719"
     CDS             complement(96..662)
                     /locus_tag="IW774_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1155037.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(662..1618)
                     /locus_tag="IW774_002720"
     CDS             complement(662..1618)
                     /locus_tag="IW774_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1155038.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1618..>1957)
                     /locus_tag="IW774_002721"
     CDS             complement(1618..>1957)
                     /locus_tag="IW774_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1155039.1"
                     /translation="FFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQ
                     KRTLRTLLQLLMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQ
                     IDNSYRKKDF"
BASE COUNT          555 a          420 c          370 g          612 t
ORIGIN      
        1 agcaccgtga ataaagaggt tatgaaatca acgctctaac ctgagtatta gagaaaaaaa
       61 cgcaaaacat atcatttatg ttttgcgttt tttatttatc tttttttctc aatacaatat
      121 aaaataggcg gctggttctt ttggttgata aaccgataat tcagcacatt atatgtttcc
      181 tgtggcagtt tggaacaaaa agaatcaacc atatccagtt ctttttctcc accttcatga
      241 ccatagtaga cgacaaggat caatcgtccg cgaggcacaa gtcgtttcaa tatttcttcc
      301 atagctgtgc gtgtagtctc tggcatagtg atgattgctt tatcactttt tgggagatac
      361 cctagattga aaatccctgc tttgagattt tgttgttcag ctatcacttc tcctaacgtt
      421 tcgtggcctt gtagaaacaa tgagacacgt tcttctaact gacgctgttc tagcctttca
      481 cgagtagcat cgattgcttg ttgctggata tcaaaactat agacatgacc agtttttccg
      541 atgtgttctg ctagaaaaac cgtatcgtgg ccattgccca tcgttgcatc tacgacgtga
      601 tcccctggtt gcaagatttc ttgcagtaat gtatggctga aatgcaaagc agtttgcaac
      661 atcagacgct cacctttttc cgtacatcat attttccttg gaagctatct ctgcgaagaa
      721 gttcttcatc aattgcattc aagacttccc attttttcaa gctccacatt ggtccaatca
      781 aggaatccca cggcgcatcc cctgtcaaac gatggatgat gatctctttt ggtatcattt
      841 ccagctggtc gcaaatgaca ttgacatagt caggacggct cattaattga aggcgacctt
      901 catgatagtc ccgcaacatt ctcgttttgc gcatcagatg catcaaatgg agcttgattc
      961 cttggatatc tgaatctaaa atggtgcgtc tgacattttc tttcatcatt tctaaacttt
     1021 cgcctggtaa tccgttgatc aaatgcgtac aaacacggat attatgtttg cgtaatttag
     1081 ccacgccatc taaataagtt tgataatcat gtgctcggtt gatcagtttt gaagtttctt
     1141 caaaagttgt ttgcaatcca agttctaccc ataaatacat ccgctcgttc agttcagcaa
     1201 ggtaatcaac tacttcatct ggcagacaat ccggtcgtgt accgactgat aatcctacga
     1261 cacctggtaa attgactact tgctcaaatc gttctttgat tatttcaact ggcgcatggg
     1321 tgtttgtaaa attttggaaa taaacgatgt attgttggac atgaggccat ttttgatgca
     1381 tcatgtcgat ttctttctgg aattggattg gaagagggtc ttcaggtgca acgatcatgt
     1441 ctcccgaacc agatacactg caaaaagtac aaccgccatg ggcaacagtc ccatctcgat
     1501 tcggacagtc aaatcctcca tcgatcggca ccttgaaaat tttcccgcca aactcatttc
     1561 gtaaagcgta attccaagaa tgatatcttt tatttccttc tgaataagga aaagtcatta
     1621 aaagtccttc tttctgtaag agttatctat ttgttttctt ttttctctat acactggata
     1681 agtcaggcac agccaagaaa gactgagtaa gtagccgccg atgatatcag taggaaaatg
     1741 aacgcccaga taaatgcggc ttatcccgac acttactata ataaacatca tcagcaattg
     1801 aagcaatgtg cgtaaggttc ttttttgaac aaaagctgga agcagaaata gtaaagaacc
     1861 gtaaagaatc atactgcctg ctgcatgtcc actagggaaa ctgtaacttg tttcaactac
     1921 taagtgcgcc aatgaaggac gctctcgcat gaaaaat
//
LOCUS       DACEOE010000272         1946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.283, whole genome shotgun sequence.
ACCESSION   DACEOE010000272 DACEOE010000000
VERSION     DACEOE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.283"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            592..1920
                     /locus_tag="IW774_002722"
     CDS             592..1920
                     /locus_tag="IW774_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1155040.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
BASE COUNT          653 a          320 c          383 g          590 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgta
//
LOCUS       DACEOE010000273         1920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.284, whole genome shotgun sequence.
ACCESSION   DACEOE010000273 DACEOE010000000
VERSION     DACEOE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.284"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1918
                     /locus_tag="IW774_002723"
     CDS             <1..1918
                     /locus_tag="IW774_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1155041.1"
                     /translation="LRIIAGAGSGKTQTICAKAVYLMTQKQVDEERILMITFTRNAAN
                     ELKKRVDNFSQRKTAVHIGTFHSIFFRIYNEICRKFPEVAMQGIQGDFSKDTAQKVNA
                     VLQQLIRKYNLYIFDQYGEKTIASRLDYWQNMNLSVEEMVQLVKEKYDSIDKNARQPI
                     SERLYGLLTELQEQKRSQQLLEFNDVLQNLKSALENEEIQHYIGQKYDYLFIDEFQDT
                     NPLQWEIVKRMTKENRIKLIVVGDDDQSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNY
                     RSRAAIVQGANRLISYNKNDRIPKAMIPAQKEAGVMEAYLFSDTKAESQWLIKQIQTF
                     IQKNGKYKESIILYRSASQTQQLVQSLLKTNIPFVLEADSPYEGIFGIKSFQHFYQKI
                     VHWQTALNLQKKNQAYQQLLRQLMADCYLKKSECDQYFSPKYQNISVADYILSISPNL
                     KSKSAEIREFEEALRKTDQHQEIYSLVQSYLHLPRIAKEIDQSDQEWIREEVKQHATF
                     RRLQALSQETQATAKELKTRLIAYRQGKLDALCIQSIHKSKGLSYRNVFLIGCNEGSL
                     PYNGATEKKVIDFNEIKAEPVTTIEEERRLFYVAMTRARNRLYLCVPQMKNSRKLKVS
                     RFIKETGSRVKKGK"
BASE COUNT          724 a          333 c          376 g          487 t
ORIGIN      
        1 tcttcgaatc atagcaggag ctgggagcgg caaaacgcaa acaatctgtg caaaagctgt
       61 ttatctgatg acgcaaaaac aagtagacga agaacgcata ctaatgatca cttttacaag
      121 gaacgctgca aatgaactaa aaaaacgagt agataatttc agccagagaa aaaccgctgt
      181 ccatatcgga acattccata gtatcttttt taggatatac aatgaaattt gtcgaaaatt
      241 tcctgaagta gcaatgcaag gaatacaagg agacttttca aaggatactg cacaaaaagt
      301 gaatgctgtt ttgcaacagc tgatacgtaa atataatctt tatatatttg atcaatatgg
      361 agaaaaaacg attgcttctc ggttagacta ttggcagaat atgaaccttt cagtagaaga
      421 aatggttcag ttagttaaag aaaaatatga ttccattgat aagaatgcac gacagccgat
      481 atcagaacga ctgtatggat tgctcacgga actccaagaa caaaaaagaa gccagcaatt
      541 gctagagttc aatgatgtac tccaaaatct gaaaagcgct ttggagaatg aggagatcca
      601 gcattatatc ggccaaaaat atgattatct attcattgat gaatttcaag atactaatcc
      661 acttcaatgg gaaatcgtga aacggatgac aaaggaaaac aggatcaagt tgattgttgt
      721 tggagatgat gatcagagta tctatggatt cagaggatca gagccagcat acatcaagaa
      781 ctttgaaaaa gaatatccta ccaaaactct tttcttatta actaattatc gttctcgggc
      841 agctattgtc caaggagcaa atagattgat cagctataat aaaaatgatc ggataccaaa
      901 ggcgatgatt ccagcacaaa aagaagcagg tgttatggaa gcctatttgt tttctgatac
      961 taaagcagaa agtcagtggc taatcaaaca aattcagaca tttattcaaa aaaatgggaa
     1021 atataaggaa tcaatcatat tataccgatc agcttcacaa acgcagcagc ttgtccaatc
     1081 tctgctgaaa acgaatattc cttttgtact ggaagcagat agtccatatg aaggtatctt
     1141 tggtatcaag tctttccaac atttctatca aaaaattgtt cattggcaaa cagcactgaa
     1201 tctgcaaaag aaaaatcagg cctaccagca attgctccgg cagctgatgg cagactgcta
     1261 cctgaaaaaa agtgaatgcg atcaatactt ttctcctaaa tatcaaaata tatccgttgc
     1321 agattatatt ttgtcgataa gccccaattt aaaatcgaaa tctgcagaaa tccgagagtt
     1381 tgaggaagcg ttgcgtaaaa cagaccagca tcaagaaatc tattcgcttg ttcaaagcta
     1441 tcttcatctg ccacgtatag ctaaggaaat cgaccagagt gatcaagaat ggatacgaga
     1501 agaagtaaag cagcatgcta ctttcagaag actccaagcc ttatcgcaag aaacccaagc
     1561 gacagctaaa gaattaaaaa cccgcttgat tgcttacaga caaggaaaac ttgatgcact
     1621 atgtatccag agcattcaca aaagtaaagg tcttagttac cgaaatgtct ttttgattgg
     1681 ttgtaacgaa ggaagtttgc cttataacgg agcgacagaa aagaaagtaa tcgactttaa
     1741 tgaaataaaa gcagaaccag taacgacgat agaagaagaa cgaagactat tttatgttgc
     1801 gatgactaga gcaagaaatc gtctgtatct atgtgtacct caaatgaaaa attcaagaaa
     1861 attgaaagta tcgagattta tcaaagaaac cgggagtcgt gtgaaaaaag gaaagtgata
//
LOCUS       DACEOE010000274         1910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.285, whole genome shotgun sequence.
ACCESSION   DACEOE010000274 DACEOE010000000
VERSION     DACEOE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.285"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..627
                     /locus_tag="IW774_002724"
     CDS             <1..627
                     /locus_tag="IW774_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1155042.1"
                     /translation="GINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEY
                     YQSEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQ
                     EEWERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKV
                     FFYPILVNKEKESWENLRTDVLFKCLSGEYVDIEQKFIDTFWKNLTNK"
     gene            640..>1910
                     /locus_tag="IW774_002725"
     CDS             640..>1910
                     /locus_tag="IW774_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ1155043.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IISDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIEPEKYKRILLVDVHGVKGG"
BASE COUNT          709 a          221 c          387 g          593 t
ORIGIN      
        1 ggaataaaca gatttttagg tttagttaaa acattagaaa aaaatgatgt aacatccatt
       61 aatcgattaa agcactgggt atctctaaca aaagaattgt ctaatccatc tttgaagagg
      121 gaaatagaat attatcagtc agaggattta ttaaaagctt taaaatggtc ggaagaagtc
      181 aatcaacaga ttagtcaagc taaaggatta gataaaccct ttttaagtgc tagagatact
      241 ataaaagctc tgaaaaaata tgggaagata attattgtaa gctcagctaa taaggaagct
      301 gttcaagaag aatgggaaag acatgaatta ttatcattag ttgatgaact gtgttgtcaa
      361 gataaaggaa aaaaagaaga cattattcga agtgtaattg aaaatggttg tgatttggat
      421 aagatattaa tgattggtga ttctcctggt gatctagaag ctgcaaataa aaataaagtt
      481 ttcttttatc ctattttggt taataaagaa aaagaatcat gggaaaactt aagaactgat
      541 gttttattta aatgtttatc gggagagtat gtagatattg aacaaaaatt tatagatact
      601 ttttggaaaa atcttactaa taagtagagg agaaagaaaa tgactataaa agtagactta
      661 cgaaaaaaac catataattt agatgaagat gctatttctt ggattgaaga gacgattgat
      721 tctatgactt tggaagaaaa aataggtcaa ttgtttgtta atatgggatc tagtcgtgat
      781 aaaaattact tgagtaatat ggtcaatcag taccatattg gagctgtacg atataaccca
      841 ggaactgctg atgaggtata tgatcaaaac tatatcctac aaacagaaag caaaattcct
      901 ttattaattg ctgctaatac ggaagcaggt ggtaacggag cttgtacaga tggtactgaa
      961 gttggtgtag aagtaaaaat tggtgcaaca aatgatgcga aatatgctta tgaattaggg
     1021 agagtttctg ggattgaagc atcggctatt ggatgtaact ggagttttgc tcctattgta
     1081 gatattaata gaaattggag aaatcctatc atttctagtc gatcttttgg acaaaaccct
     1141 gataaagttt tagaacttag tttagcttat atgaaaggta ttcaggagag tggaattttg
     1201 cctgcagcta aacatttccc tggtgacggt attgacgaac gagatcagca tctatctttt
     1261 agtattaata gtttaggtac ggaagaatgg gataattctt ttggaaaagt atactcaggt
     1321 ttaatagaag ctggattacc ttcgattatg gcaggtcata tccatatgcc agcttattca
     1381 aaacatttta atcctgatct gaaagatgaa gatttacttc ctgcaacttt atcaaaagaa
     1441 ttaatcactg acttacttag aggaaaactc ggatttaatg gattagtagt tactgatgct
     1501 agtcatatgt taggaatgac tggggcaatg aaacgatcag aattattgcc cacttctatt
     1561 gcagctggat gtgatctatt tctatttttt aatgatccag atgaagactt taattatatg
     1621 atggaaggtt atcgtaaagg aattatctct gatgatcggt tattagatgc cttaaaaaga
     1681 attttagggt taaaagctaa attgggatta cacaaaaaag caaaagaaca aattttatta
     1741 cctaagcaag aagctctgga aaaaattggt ttaattgaaa ataaatcaat ttttaaagag
     1801 gtggctgacc aagcaattac attagttaaa gataaacaag atatttggcc tattgaacca
     1861 gaaaaatata aaaggatttt actagtagat gtacatgggg taaaaggtgg
//
LOCUS       DACEOE010000275         1873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.286, whole genome shotgun sequence.
ACCESSION   DACEOE010000275 DACEOE010000000
VERSION     DACEOE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.286"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            612..1424
                     /locus_tag="IW774_002726"
     CDS             612..1424
                     /locus_tag="IW774_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155044.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          647 a          262 c          305 g          659 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa taacttttat
     1681 attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag attagataga
     1741 tattataaat atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata
     1801 aaagaaggta tcaagagtca cggtgaattt attccgaaaa gtgaaaagaa caaatataaa
     1861 atttaataaa aat
//
LOCUS       DACEOE010000276         1871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.287, whole genome shotgun sequence.
ACCESSION   DACEOE010000276 DACEOE010000000
VERSION     DACEOE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.287"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>1871)
                     /locus_tag="IW774_002727"
     CDS             complement(<1..>1871)
                     /locus_tag="IW774_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1155045.1"
                     /translation="NRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQYFILWINPIA
                     TTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITVKSVLGFSKV
                     SQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPMAIAVTCLSG
                     LIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSSVRAHASSNG
                     IDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQEVTPFLNSIF
                     QNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVFQAAPAILGQ
                     KQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGILDKDLFRESA
                     QYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTAHYLDQSLEQ
                     FFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDNAQMQRVPLM
                     IHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHDQVVAFRNGN
                     FVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDKLNQENLLR"
BASE COUNT          576 a          380 c          337 g          578 t
ORIGIN      
        1 ccgcaagaga ttttcctgat tcagcttatc tgaaagactt aacgcagtct tgactttttc
       61 ctgatctttg gagatttcct catcgattcc tgctgctttc gtatcgatga tttctcctgt
      121 ctgattattg taagcttttc ctcctaggac ggtgtatttg ggtgtcacaa agttcccatt
      181 tcgaaaagca actacttgat catgctgaga agataataag tctgtaccaa aatgaagata
      241 atctttatca tcgattccca atagatgtag taatgtcggc aaaacatcga tttctccacc
      301 gtattcatga ttgactgtcc ctttagtata accaggcata tggatcatca atggcactcg
      361 ttgcatctgt gcgttgtcaa attcatccca tgtatctgga tctttaccaa gagcgcttgc
      421 taaatcttta ttatctgtat tcgagatccc aaaatgatcc ccgtaaatca caaagattga
      481 attttggtaa ataccagatt ttttcaaata cgtaaaaaat tgttccaatg attgatccag
      541 ataatgtgcc gttcgaacat aattatctac tgtgctattg ccagtattca aagatggaaa
      601 gtcgaaattc ttgtcatccg tataataagg cgtgtggttg gttacggaaa gaaatttcgt
      661 gtaaaacggc tgttgtagat gctcaagata ttgggcagat tctctgaata aatctttatc
      721 taatatccca taacctaacg tttcgtcgga ttcatcaaaa taagatcgat caaagaaatt
      781 gtcataacct aagtttttat atacatgatc tctattccaa aaactagcta cattcccatg
      841 gaaaacagcg cttgtatagc cttgcttttg tccaaggatc gctggtgcgg cttgaaagac
      901 attgtctgac ccaagttctg tgaataatga gccttgaggt aaaccaaatg tgccagtttc
      961 caacatattt tcagcatcac ttgtctttcc ctgtcctact tcatgaaaga aattgtcaaa
     1021 gctgatcgtt gcttgattct ggaaaatact atttaaaaac ggtgtgactt cttgtccgtc
     1081 taccttcata ttaatcaaaa actgctggaa actttctaaa tggagaacga tcacattttt
     1141 ccctttagcg atcccaaatg tttcgggatt tggctctgca taatgtttct ttgtataatc
     1201 taaaacttca tcaatcccat tactgcttgc atgcgcgcga acactgctag tcattcctgt
     1261 tttgattccg tcataaatag tatacgcatc gattcctaga tatttgacaa tatatgaacg
     1321 gtcaaatgtt cgctgcagca attgtggacg gttggcttcg ctcagcgcta gatttcctga
     1381 aaaaataagc cctgaaagac aggtaacagc gatagccatc ggtttgccga cagggttgct
     1441 tttgataggt attttcttga ccttcaacca aaccagtagc ccaatgaaca cagcgatatc
     1501 cagccagtag atgacatcat ggggcttcat caaagaaaag gagcttcctg acaatccttg
     1561 cgacactttc gaaaagccta atacagattt gactgtaatg aaatctgtga attcccgata
     1621 aaaaataata ttcaagtaca acaaaagcgt atttaaaata tcaatgatca aaagaatagc
     1681 taacgctggc tttggctttt taatatacaa agaaaggcca aaaaacaata aggtagtcgc
     1741 aattgggttg atccacaaga taaagtactg gatcgtacct tccacaccta atgaaaaatc
     1801 tgcatagtag gcaatgatcg tcttcagcca gaaaaagaaa acagctgtca aaacgattgc
     1861 taaacgattt t
//
LOCUS       DACEOE010000277         1851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.288, whole genome shotgun sequence.
ACCESSION   DACEOE010000277 DACEOE010000000
VERSION     DACEOE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.288"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..1204)
                     /locus_tag="IW774_002728"
     CDS             complement(26..1204)
                     /locus_tag="IW774_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1155046.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
     gene            complement(1576..1839)
                     /locus_tag="IW774_002729"
     CDS             complement(1576..1839)
                     /locus_tag="IW774_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ1155047.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          577 a          314 c          312 g          648 t
ORIGIN      
        1 aattgtcctc ctgcaaaata attagttact cagtaaacat tgaaactaat gtatcggtta
       61 cctgttgaaa acctttatgg cttctgttta gaaatttttg attgtatgta tcaaaaatgc
      121 tgactagaaa gcgttctagt gattcttcat tttgaaactg ctcttttcta cggctgtatc
      181 ttttaatttg cttattgaaa gactcgatta gattggttga gtaaatggtt ctacgaatgc
      241 taggtggaaa atcataaaaa gttaataagt cttggttttc tatgagtgac tgcgtcactt
      301 taggatagtt tttcttccat ttctcaatca tgccggataa gaaggtattc gcttcttctt
      361 ttgagttagc ttgataaaca gccttaaagt catcacagat ttcttttcgg tctttgacac
      421 gtactttatg agcgatgtta cgagatacat ggatacaaca atgctgatat tttgctttag
      481 gataaatttg atggatagta tctttcatgc cttttaagcc gtccgtaata aaaagcaaga
      541 cttcttgaac tcctctggag ttaatatcct gtagcagctc attccaaacg tatgttgatt
      601 cagttggagc aatcgcataa ctcagtactt ctttagtgcc gtcttctcgt ataccaatgg
      661 caatataaat cgcttctttg gatacggttt gacgttttag tggaatgtaa gtagcgtcca
      721 taaaaatagc gacatactta tcatttaagg ctctggattt aaaggcattt acttcttcag
      781 tcagaacttt agtcatgttg gacatggttt gtggagtata gtgatgaccg tacatttttt
      841 cgatcaaatc agcaatttca gacatcgtaa cacctttttc gaataaatgg ataatagtgg
      901 tttccaatgt atcgtttgtt cttttgtagg ctggtaaagt ttgttgttta aactcaccat
      961 tacgatctct aggtatttcc aatgttaatt caccatattc ggttttgatt gatcgaaagt
     1021 aagaaccgtt tctcgaatta cctgaattaa aaccagtgcg atcatatttt tcgtaatcta
     1081 aaaaagccgt taattcagtc cgtaggagtg tgtttatcgc tttttctaag tgcgaacgga
     1141 ataattcatt taaatcgcct ttagtgacta gagtttgcac aatttctgta gtaaaatcat
     1201 tcatagggaa gtcctctttt ctgtgaattg gttgtcgtta actttattct acagaagcgc
     1261 cttccttttt tgtatggatt ttttcattta cacaaaatat tttatactct cctgaatttt
     1321 ttcatgttga aaacatcact aatcaaaggc aaattataat ttagaaatag aatgaaaaca
     1381 ctaaatgaaa atagaacatt agaaaaaata gaatcaaaaa aacccaagac tagtgccgaa
     1441 ataagggaaa caatcgaagc tacaaaaaat aatgatttcg ttgaacagga ccaatttatt
     1501 tgtttccgat atttctcaaa aagaactttc tttgggaaag ttaaaggcga ctgtaaagtc
     1561 gcctttagtc cttttttacc aagctgctgc atatttagct ccatattttt taattaatga
     1621 tttggctgta gcaacaatac cagctgtaat taatccgcct ccaacaactg cagcaacaat
     1681 agaaatgatt gtagcaaccg tgctaccagt attaattatg tcaataactt ttttagctgc
     1741 ataagaagaa attccaatgt tagcacttag cattggtaca ccaacaaaga atcccgcaaa
     1801 acctaaaatt ggtaaaacta ataataaatt ctttttcata ataattcctc t
//
LOCUS       DACEOE010000278         1838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.289, whole genome shotgun sequence.
ACCESSION   DACEOE010000278 DACEOE010000000
VERSION     DACEOE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.289"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            263..1819
                     /gene="rny"
                     /locus_tag="IW774_002730"
     CDS             263..1819
                     /gene="rny"
                     /locus_tag="IW774_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1155048.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT          647 a          310 c          417 g          464 t
ORIGIN      
        1 taatagaaaa gtttctcaat ttaaaggttt acctatatga aactagtgtc atttatttta
       61 aaaaccatcg tttaatgaat aagtagaaag ccgtttcttt gtttatttaa agttgacact
      121 acggggtaca aacattagaa taaagttgtg catgtttttt gtacacatat atgcgaagat
      181 gcatattgac gagtttagaa taatttatca tagaagctgt atagatgata aaaatgaaaa
      241 agatagtatg gaggtggatt ttatggtatt caacattctc ctcgctatca tcggtttaat
      301 tgtcggtctt ggtttagggt ttatggtcgc aaagtcacgt cacgagaaag aaatagctgg
      361 tgcacaatca tcagctgctg gaatcatcga tagtgcaaaa aaagaagcag aaactctaaa
      421 aaaagaagca ttgttagaag ctaaggaaga aaatcagaaa taccgttctg aagttgaaag
      481 tgagctgaga gaaagtaagt tagaattaaa atctcaagag aatcgtttga ttcaacgcga
      541 acaaacgctg aatcgtaaag atgactcgtt agaaaaacgc gagaactcac ttgaaagtaa
      601 agaagaagca cttagttcaa aacagcaatt aattgaagag cgagaaaaag acgttgaaaa
      661 aatgattgaa gatcaacaaa aagaactaga aaaaattgct ggtttatcaa aagaggacgc
      721 acgagatgtc atcatgaaat cgactgaaga agagctgaat catgagttaa ctctaatggt
      781 aaaagaatct gaacagcgtg ccaaagaaga agcagatcgc aaagcgaaaa acttattatc
      841 attagcgatt caacgctgcg cagcagatca agtttcagaa acgactgttt cagtcgttac
      901 ccttccgaat gatgaaatga aaggtcgtat cattggacga gaaggtcgta atattcgaac
      961 gcttgagacg ctgactggta tcgatttaat catcgatgat acaccggaag cagttgttct
     1021 ttccggattc gatccaatcc gaagagaaat cgcacggatg accttggaaa aactgataca
     1081 agatggacga atccatccag cgcgtatcga agaaatggtc gagaaatctc gtaaagaaat
     1141 ggatgaacgt attcgcgaat acggggaaga ggcagcgttt gaagtcggtg ctcatacact
     1201 tcatccagat ctaatcaaga tcatcggacg tctgcgattc agaactagtt atggacaaaa
     1261 cgtcttgaag cattcggtcg aggttgcaaa attagctggt atcttagcag cagagcttgg
     1321 cgaagatatc caattagcaa aacgtgccgg gttgctccat gatattggga aagcacttga
     1381 ccatgagatt gaaggatctc acgttgagat cggtgcggaa ttagctgcca agtacaaaga
     1441 aaacccagta gtaatcaacg cgatcgcttc tcatcatgga gatgtggaag ctacttctgt
     1501 tatctctgtc cttgtagctg cagcagatgc gctctcagca gcaagacctg gtgcgcgaag
     1561 tgaatcatta gaaaactaca tccgtcgttt aagaagtctg gaaaatattt cgaacagctt
     1621 cgccggtgta gaatcaagtt acgcagttca agctggacgc gaagttcggg taatggtcaa
     1681 gccagaagaa atctcggatt tagacgctgt tcgtttagta cgtgatattc gtaaacgaat
     1741 cgaagaagaa ctggattatc ctggacacat caaagtaatc gttattcggg aaactcgtgc
     1801 aactgattat gctaaataat aaaaaataca aaagaaga
//
LOCUS       DACEOE010000279         1810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.290, whole genome shotgun sequence.
ACCESSION   DACEOE010000279 DACEOE010000000
VERSION     DACEOE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.290"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(12..287)
                     /locus_tag="IW774_002731"
     CDS             complement(12..287)
                     /locus_tag="IW774_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1155049.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            395..1159
                     /locus_tag="IW774_002732"
     CDS             395..1159
                     /locus_tag="IW774_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1155050.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1282..1785
                     /locus_tag="IW774_002733"
     CDS             1282..1785
                     /locus_tag="IW774_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1155051.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
BASE COUNT          579 a          330 c          401 g          500 t
ORIGIN      
        1 ttttcacaac cttatcccac cacatcaaat ttctttcgtt cctcaatcat cggatcttct
       61 tggacatcca catactgcac tcgtccactt gggctcggag acttcttgac ttcttcgata
      121 aatttttgga tgatgtcgtc atttccgatt gcttcgattg tgactgagcc gtcgtcttcg
      181 ttttttaccg ttccggtcac gcctaactga tcagcaacca tcttggtcat atagcgaaat
      241 cccacgccct ggacacgtcc ttggacattc attttaactt tgcgcatgtt cgtcaccttt
      301 ctttcctgtt ttataaatca atgataccga tttttttagg atgaaacaag ctcaagcacc
      361 tattccatga tataatcagg tgaggtgaag gaatgtgaaa gagatccaat cagcaaaaaa
      421 tccaagaatc aaagaattaa aaaaactaca taaaaagaaa tacagagaag aaaaggcaga
      481 atatcttttg gaagggttcc atctgatcga agaagctgcg tcgtctgatg cagaaataaa
      541 agaacttttt gtgacacaga gaggtatgaa agaatgggga gactggctgg ctgtaaacaa
      601 agagatttct acttttctag tttcagatga ggtcatgacc gctatgtcag atctacctac
      661 accgcaaggg atcttagcag tgatgacgtt aacatctgaa gatacgccag atttttcagg
      721 aggctggctt ttactagata atgtacagga tcctggaaat gtgggaacga tgatccgtac
      781 ggcagatgcg tttggtatgg ctggtgtttt attaggtaaa ggaacggcag atatttatag
      841 tacaaaggtg ctgcgaagca tgcagggaag caattatcat ttgcctgtgt tacaaaaaga
      901 tttggcagat gtaatccctg taatgaaagg caaagggttt acggtgtacg gtacagaatt
      961 aaatgaagaa gcacatgcat tagacaaagt agaaaaaaca gaggatttcg ctataatcat
     1021 gggaaacgaa ggtcagggag tcagtcagga aatcttgtct ttgactgatc agaacgtata
     1081 tatcccaatg aaaggaaatg cggaatcatt gaatgtcgga gtcgcagcag ggatcgttct
     1141 ttatcatttt tctttatgat taaggaacta ttaagcttta gctattttat ctagagcgac
     1201 tagaataatc cgaacactgg tggtatactt agatcaattt cagaagaaaa tgaaattcaa
     1261 aaaggtggca gatcgataat tatgacagaa aaatggcgtg aagacgaaga atatatgtct
     1321 tatgtacaag atcttttaga aacagaagca gtgaaaaaat tagcgaatta cacacagcat
     1381 gttcattcca ctcgtttgga gcattccatc agtgtttctt actatagtta tctgctcgca
     1441 aaaaaatggg gtggcaatgc taaagcaaca gcgcgtgctg gacttttgca tgatctgttc
     1501 tactatgatt ggcgtacgac gaaatttgac gaggggacac atgcgtatat ccatccaaga
     1561 attgctgtaa agaatgcaga aaaaatcacc gatttgtcgg atcttgagcg agatatcatt
     1621 ttgaagcaca tgtggggtgc aacgatcgca ccgcctaagt ataaagaagg atatattgtg
     1681 acattcgtgg acaaatactg cgcagtcaaa gaagcggctc agcccatgtc tgcaagtatg
     1741 cgtaagagat ggcaacgtta ctttgggaaa gaatcctcga tttaacaggt aaaaccaata
     1801 atagaccata
//
LOCUS       DACEOE010000280         1803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.291, whole genome shotgun sequence.
ACCESSION   DACEOE010000280 DACEOE010000000
VERSION     DACEOE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.291"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            118..315
                     /locus_tag="IW774_002734"
     CDS             118..315
                     /locus_tag="IW774_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155052.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            421..903
                     /locus_tag="IW774_002735"
     CDS             421..903
                     /locus_tag="IW774_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155053.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(961..1557)
                     /locus_tag="IW774_002736"
     CDS             complement(961..1557)
                     /locus_tag="IW774_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1155054.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          556 a          275 c          360 g          612 t
ORIGIN      
        1 acgctataat gcctgtaagg ttgattttta actgtaaata aaaatcaatg agttaaatct
       61 ccaaagaaat agcagtggta atgtggagaa aagttccagc agtttaggag gattttgatg
      121 acggaagaaa tgattaattt aggagaacaa tatgcatgca aaccaattgg ttttacaaaa
      181 acggtcattg gtgaagttgt atcaaaaatg acaaactgcg ccgtggtcaa agttgcccaa
      241 tgtgctgcag aggaccaaga attattagat gaaaaagcaa gcatggttgt tgcaaaatat
      301 gatacatttg aataaagaaa acaattggac gtgcggcgtg ctgcaagtcc aatttctttg
      361 agaaggtaaa tcagtattct tttcctattt tctggagaag aaaaagcgag gcgagttgtc
      421 atggtaacat tctttacggt tctattgttt ttattcgttg ttgtgatgtt tttatttctt
      481 tggctggttc gaaaagaaat tatttatcgg actgtgcgaa atcgatgggt ctacttggtg
      541 atacccttct tagtgtcact agtcatttgg tatacactca tcagtcatcc tacagcagat
      601 gaattggcaa aagggatctt gtcagcaatg atttttatta gttttttgtt ggacagtaga
      661 ggaatcacag aagaaggact tgttttgaat agctttgaca aaaaaggtgt tcctttttct
      721 gagatcaata aaattgtatt atatcagcca aaaggttcta aaatcgtaaa aatgaatttt
      781 ttccgaaacg gatggcgagg accgatgctg aaattttctg catcattaga agaattagtt
      841 ccatttttgt cccaacgctt aaacgatgag gcagagattg atattatgat tgatccagaa
      901 taaaaacaga ggttgcgaca agatgtttgt cacaacctct gttttttatt tcaaataaaa
      961 ttatccaaat acgcggaagc gatcggcatc gttcatgtat tctttttccc cagccggtgt
     1021 ttctggtgaa ccgaatacta attgcgaacg tagtttccag tttgaaggga tattccattc
     1081 ttttgcaact gcttcgtcaa taactgggtt gtaatgttgc aagtttgcgc ccaatccttt
     1141 atcaactaat gctacccaag tattagctgt tgcgattccg tttgattgtt ctgaccaatc
     1201 tgggaagttg tctgcatata attcaaattg ttcttgtaga ccttttacca cgtcagtatc
     1261 tttgaagaat aatactgtac cataaccatt tttgaaacca gctaatttgt tttgtgtatt
     1321 tgggaaagct tctgctggag tcaatgggcg aagtgcttct tcagtgattt cccaaagttt
     1381 ttcatgtgca tctccgaaaa ggataactgc gcgtgttgat tgagcattga aagcagttgg
     1441 gctttcttta attgcttctt tgatcaaagt tgttaattct tcattagaaa gtgaaacatt
     1501 acgtcctaga tgatagattg aacggcggtt tttcaaagtg tcagtaaaag ttgtcataat
     1561 aaaatttctc ctttgatttg cgttttgcat ttttttaatt gattacgaaa tgaagtatag
     1621 caccttacaa aaagtaagta aacagatatg ctcacaaaaa ataagcgaag ttcaaaaaac
     1681 aattttaata agaaaaatcg ttatgtaagg attttcttag ttatcgcttt cattgttgtg
     1741 ttttaggaat ttttgcttat ttgaaaaaaa cagctgatat ataattatta aatctctatc
     1801 ttc
//
LOCUS       DACEOE010000281         1801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.292, whole genome shotgun sequence.
ACCESSION   DACEOE010000281 DACEOE010000000
VERSION     DACEOE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.292"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            77..1237
                     /locus_tag="IW774_002737"
     CDS             77..1237
                     /locus_tag="IW774_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1155055.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1253..1696
                     /locus_tag="IW774_002738"
     CDS             1253..1696
                     /locus_tag="IW774_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1155056.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT          629 a          286 c          363 g          523 t
ORIGIN      
        1 ggctttcatt ttctattttt ttcctgaacc catataataa aagaaagaac aaaaaaactt
       61 gcaagggagg acacatgtga agcagagtaa gcaaaaacag tctcgtcgac ttttgtttcg
      121 tttgttttta ttaatatgta tcgtcgtagg gttactgatc gctttatatc cattttatgt
      181 ggattcttta aatagtttta ttgatcaaaa acgtatcgaa caagtacaaa aacggaccgc
      241 agctgaaatt gaagtgcagc gaaagaaaat ggaggagaaa aaccaacgat taactgacca
      301 aggattcaat cctggtactg atcctttcaa tgaaaagaac agaaatgaaa gtacaacatc
      361 cagccaatta gaggaatggc tgatcggaag tatcaatata cctaagatcc aattaaatat
      421 ttcactttat gatcggctca atggtaggat ccttgaaaat ggagcaggcg tcttgcaagg
      481 aacctctttc ccgcttggtg gaaattcgac gcattcagtg atctctgctc acagcggttt
      541 acctaatcga cggttattta ccgaattaga tcgtttagaa aacggagaca cgtttatttt
      601 gactgttttg ggggagaaat tggcttatca agttaaaaat atacaagtgg tactgccaga
      661 tgacacgtct gttctaacga tttcagaagg agaagactta gttaccttgc ttacatgtac
      721 gccgtatatg atcaacacgc atcgtttgct agtgacagga catcggattc cttataacga
      781 atcggtaaaa aaagaagaag gaaaaggaaa tcaggaaaga accatgcggc aatggtttat
      841 tcttgcagga acgataatag ctgtagtcat actgttagtg tttatcggac gactaatcta
      901 tcagtataga ttaagtaaaa aagtgcttga tttttcattt attattagtg attcgtcagg
      961 gaaaccggta aatggtagtt cattcatttt gaaacataag aaaaaaacac ttactagaaa
     1021 tggtgtgccg ttttcagtac aaagcgacca tcatgggaaa gtcaagctgg atcaattgcc
     1081 tggtggaact tatcgaatcg tttcagctga tgatcctaaa gttgctgttt cttttggtat
     1141 tagaaaagta aaacaagaga agatatactt ttttgagggg cgaaaacttg taaaagagct
     1201 tcaaaaaaat ggtttttatt ttaaaatcaa tgaataagta aggagagaaa aaatgatccg
     1261 cacaacaaca aaattgtctg ccaaagaact ggaatctgtt ctatcgattt ggctgcatgc
     1321 taacttggat tctcatgaat tcattccatc aacttattgg gagaatcaag tagaatctgt
     1381 taggaaagct ttaccagatg cagtgattta cttagcagaa atagatcaag aaattattgg
     1441 ttttgctggg gtgattgatc agtttatcgc aggaatcttt gtgaaaaggg aataccggca
     1501 acaaagaatc ggtacgcaac taatgaaaaa aataaaagaa accaataaaa cactgacttt
     1561 gacagtctat caaaaaaatc aatcagctat tcatttctat ctttcacaag gttttacaat
     1621 tcaaacacag caaaaagatg aagcagtaaa tgaggaggaa cttgtgatga aatggaatca
     1681 aaacgctgat ttttgatgaa cagaataacg aaataatgct cccagcctaa ttttttgctg
     1741 ggagcattat ttcattttga agtcactaat ttaactgtgt gaatatgcag ttgttacaat
     1801 c
//
LOCUS       DACEOE010000282         1773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.293, whole genome shotgun sequence.
ACCESSION   DACEOE010000282 DACEOE010000000
VERSION     DACEOE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.293"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..403
                     /locus_tag="IW774_002739"
     CDS             5..403
                     /locus_tag="IW774_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1155057.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IW774_002740"
     CDS             387..1082
                     /locus_tag="IW774_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1155058.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..>1773)
                     /locus_tag="IW774_002741"
     CDS             complement(1666..>1773)
                     /locus_tag="IW774_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1155059.1"
                     /translation="EAKTLDTEFMDKTIRKVMKKDNVTIKDPYVKNIFK"
BASE COUNT          652 a          259 c          343 g          519 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttc
//
LOCUS       DACEOE010000283         1764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.294, whole genome shotgun sequence.
ACCESSION   DACEOE010000283 DACEOE010000000
VERSION     DACEOE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.294"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(98..358)
                     /locus_tag="IW774_002742"
     CDS             complement(98..358)
                     /locus_tag="IW774_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1155060.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(385..1686)
                     /locus_tag="IW774_002743"
     CDS             complement(385..1686)
                     /locus_tag="IW774_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1155061.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT          650 a          374 c          272 g          468 t
ORIGIN      
        1 taagccaaca tttcacaaaa atttaagaag caattttcgt aaatttcttc ttatttcttg
       61 aagctaacca cttctgttac aaccactttt agaacattta aagcttcgat gtgacaaacc
      121 atctatgctg ctcgatcata aaattgatag caaaaattcc acaaagtaca tatgtccccc
      181 atgaaaacaa aacattcata tgcggtacaa tgatctttgg aagaatcacg atcaaaaatg
      241 atgccagtgc ccaaatccat cgattgattt gaatatgata tgtagctagc atattcaata
      301 taaggtgact agccgttaat aaaaagataa aaacaaaaaa taaaatcagc caatacattg
      361 tttcccacct tctaaaaacc aaacttagac agtctggact tcgttttctc cacgactttg
      421 ttcatctgac agcatctttt tatcataagc ttttacaaat ggataataga taataaatgc
      481 tatcaatgtg ttgatcatga tcaagataac aacacgccag tcatttcctg ctgaaagata
      541 accgccgatt ggagctggta atgtccatgt tggcaaaaca gtaaatctat tgaccatttc
      601 taatttgact gcaaaataac tgataatagt caaaactaaa ggaactaagt tgaacgggat
      661 gaacatgtaa ggattcaaca tcataggagc tccaaaaatt atgggctcat tgatattgaa
      721 gataccagga ataatagaaa aacgaccaac ctctttcaag aattttgatt tacagaagaa
      781 tataaacaaa acagatacca cgatcgttgc tcctgcacca ccaattgtta ccgtccattg
      841 ataaaactgt tcaggagcga tatagggtaa ttgggtggca gctgtaccat ctgccaatgc
      901 ctttgcattc tcatccaaca tgatcagcca cattggacga acaacagccc ctacaatact
      961 catgccatgg ataccaaatg accacaacaa atggatcaaa aacattggta gtaaaacacc
     1021 aagcaaatta ttccctaaaa tattggttaa aggctcgaat aacgataata aggcagcgtt
     1081 tacatcaaag cccgcgacgc ttcgaatcag ccaagtaatt gttaaaatga ctgcacctgg
     1141 aatcaatgcc tcaaaactac gggcaacagc aggtggcact tgttctggca ttttgatagt
     1201 aatatttcga tttttaaaga aacgatatac ttccactgca aaaatcatag ataagatccc
     1261 cgcaaacatc ccagatgctc ccaaagaact cataggtaag acccagccaa gaggtgcctc
     1321 ttcaggagtt aaaacgttta caggaacttg taacataaag aacgttccta aagataatat
     1381 accacctgtc acactatcta atccatatga tttagctaga tccgaaccca taacaaaagt
     1441 tgcataaata gccatcaatc ccatcgtcat cctaaagggt aaaacaatcg ttgccgaata
     1501 aggggcaata aattcattcc atccaggaaa cggtaaattg ataaagacca taaaaaatgt
     1561 cccaatcaaa atcaatgaca atgttgaaac aaccccatta cgaattgcta ataaatgacg
     1621 ctgtgtacca atttttgcca ttggtgggac aagtttctct tcgacccatt tcatcagctt
     1681 ttccactaaa accactcctt ttcaataaaa tatgaaccat tcattgcatg acaacgcaaa
     1741 gaatgttcat aactaattat ataa
//
LOCUS       DACEOE010000284         1750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.295, whole genome shotgun sequence.
ACCESSION   DACEOE010000284 DACEOE010000000
VERSION     DACEOE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.295"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            516..1115
                     /locus_tag="IW774_002744"
     CDS             516..1115
                     /locus_tag="IW774_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1155062.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1179..>1750
                     /locus_tag="IW774_002745"
     CDS             1179..>1750
                     /locus_tag="IW774_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155063.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          633 a          265 c          333 g          519 t
ORIGIN      
        1 agtcaaccga tggagaatta gaaacaatct acagtaggga agaaccattg acacaaaatt
       61 attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt cttataaatt
      121 agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat gactaaattc
      181 tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt atatagactg
      241 aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt tttaaagggg
      301 caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc caatgtgaca
      361 caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct aggcagaatt
      421 gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat tatatgttat
      481 aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa cctattcgag
      541 acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat ggccaacgaa
      601 atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat ttgcgtcaat
      661 tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa gatcgattag
      721 cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac aaattgcgaa
      781 aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag caagattttg
      841 acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc caaagtctgt
      901 ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc ccacattcca
      961 tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa atcattcagt
     1021 ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt acacaagaag
     1081 acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt taaacatgga
     1141 tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta tatattatag
     1201 gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt tcaaaatggg
     1261 ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat gaaaataatg
     1321 aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt agtttgcatg
     1381 agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat tatctcatag
     1441 gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat atggtttatg
     1501 attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata cgcaaagaaa
     1561 tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat gtactattct
     1621 ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa gaaaacatat
     1681 atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat tttgacaact
     1741 ttataggaga
//
LOCUS       DACEOE010000285         1719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.296, whole genome shotgun sequence.
ACCESSION   DACEOE010000285 DACEOE010000000
VERSION     DACEOE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.296"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..533
                     /locus_tag="IW774_002746"
     CDS             <1..533
                     /locus_tag="IW774_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1155064.1"
                     /translation="SYKFIAQTLGKYMAQEEIELVYGGSYSGIMGVISKTVLENNGKV
                     TGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLVFPGGLGTLEEFSQLL
                     SWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWLDQVIFSNNPLELVNE
                     LRAVSIKNQLLLKEAK"
     gene            533..973
                     /locus_tag="IW774_002747"
     CDS             533..973
                     /locus_tag="IW774_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1155065.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            989..1690
                     /locus_tag="IW774_002748"
     CDS             989..1690
                     /locus_tag="IW774_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1155066.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT          602 a          272 c          377 g          468 t
ORIGIN      
        1 aaagttacaa atttattgcg caaacgttag gaaaatacat ggcacaagaa gaaatcgaac
       61 tggtttacgg tggttcatac tccgggatta tgggagtgat tagtaaaaca gtactggaaa
      121 ataacggaaa agtcacaggt atctatccga atgggctttt tgaaactgaa ttgcctggaa
      181 aagatgttac tacatttatt cctacagaaa cgatcgatga aagaaaggta ttattatttg
      241 aaaagggaga tgctgtcctc gttttccctg gaggtctagg tacattggag gaattctcac
      301 aacttctttc ttggatagca atcggtctga cgcccgacaa gccaatcgga atattgaata
      361 ttggcggtta ttacagcggc ttgcaacaat tattagaaac ttttgcaaaa gaggatttta
      421 tggataaaaa atggttggat caagtaatct tttccaacaa tccattggaa ctggtcaatg
      481 aattacgagc agtatctatt aaaaatcagt tattattgaa ggaggcaaaa taatgagtca
      541 aatatattta gcaggaccat ttttttcaga ggagcaaatc gatcgagtaa gtcgtatcga
      601 aaaagcgttg gaggaaaaca aaacagtcac aagtttctac tctccacgtc atcaccaaga
      661 aagcaattat gaactattca gcgctggatg ggcgcaggaa gtatacgaaa aagatatgga
      721 agaactgaca aatgcagaat ttgttgttgc tatccttgac tttgagcatc aaacaatcga
      781 tccaggaacg gcttacgaat taggtgtggc aactatgttg aaaaaaccga tgatcattgt
      841 acaagaagag acggtaccta cgaacttgat gattacacaa agtctccata cgtatttaaa
      901 atcagaccaa gctgtacgtg aatatgattt tgaaacattg cctgttgaaa cttacgtagg
      961 cgagtactta tagaagagga agggttgtat gtcagtgatt ttgatttcag gaacgattgg
     1021 tgcaggtaag agcagtttga cggatatgct tgcaaaagag attgattcta aaccatttta
     1081 tgaaaatgtc gaagataacg aagtattgcc gctcttttat tcaaatcctg agcagtacgc
     1141 ttttttattg caaatcttct ttttgaacaa gcgattttta gcaatgaaaa atgctttagt
     1201 gaacgatgac aatgtgctag accgatccat ctatgaagat agcctgttgt tccatctaaa
     1261 cgctgattta ggtcgggtaa cggatatcga agtacagcag tacgatagct tattagatac
     1321 gatgctaaac gagttagatg atgttgctcc aaaaaaacgg cctgatttga tggtccatat
     1381 caaagtttct ttggacacga tgctagaacg tatcaaaaaa cgtgggcgtg actatgaaca
     1441 gctagaaagc gatgagacgt tatataccta ttatgaaacg ttgaacaccc gctataatca
     1501 atggtatgaa gattttgatg tatgtcctaa aatacaagtg gatggggata aattcgattt
     1561 tgtagaaaat ccagaagatg caaaaaaggt gatccgtcag atccaagaaa aattagctga
     1621 attggaagga aagcctgctg gaaaatattt tacggataac cagcatgcaa aaggtcaaga
     1681 attaggctaa aaataaaaaa ccaaacgacg ggaaagaaa
//
LOCUS       DACEOE010000286         1715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.297, whole genome shotgun sequence.
ACCESSION   DACEOE010000286 DACEOE010000000
VERSION     DACEOE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.297"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..414
                     /locus_tag="IW774_002749"
     CDS             <1..414
                     /locus_tag="IW774_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1155067.1"
                     /translation="LESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSD
                     YRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAK
                     TDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            490..708
                     /locus_tag="IW774_002750"
     CDS             490..708
                     /locus_tag="IW774_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155068.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            695..>1715
                     /locus_tag="IW774_002751"
     CDS             695..>1715
                     /locus_tag="IW774_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155069.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIE"
BASE COUNT          627 a          231 c          319 g          538 t
ORIGIN      
        1 cttgaatcac tttcaactaa aaataataaa ttagatgaag aattgatcga aacatttatt
       61 gaagaaaatc ttcttaaaca aatatgggga gaaagtattg tgaattgctt aaaactggct
      121 agcaatagtg attatcgtca attcgacaac tggtacaaaa aattcaaata tgcgattaga
      181 tctgctgaaa aagaacaaaa aattcaactg aaaattatct atgaaatttg taataacaag
      241 tattttgtag atcatgttag agaacagcta tcaatgactt tacgtgactt gatacgaaga
      301 gcaaaaacag atcatcgtat caaacaaaaa gacgattata tctttgcttc tctaaaaaat
      361 aaagcgttag aactaattga attgcaaatc agcgaaggaa tagataagca ataagaatag
      421 aattgagtca aatgaaatgg aggggtatct ccacttcatt tgagaacttt gaatatagga
      481 ggggttattt tgattaaaaa tcgtttaata gatcaagttt ggtgtctaga tcaaaataca
      541 gatagtctcg aattaatcga gactatttgc ttcaacacta tagttctcga tttaagagct
      601 gaaaatgata attgtgtaac tcatgtcata attaatcaaa aaatggctca gaagctctct
      661 gagagcctca gaaaatgggc taagggaggt aacgatgaaa ataattgaaa aaaagattgc
      721 caataaaaaa ataaattggt tggttgttaa ttttcttgtt ctgatagcta tgattatttt
      781 tacaagtaat attgctcatg cgagtatttt caattggttt gatgacacaa aaagtcaaac
      841 agagtttttg aataattctc tttatagacc ttacctaaaa aagtacaatg gagatttcct
      901 tagtcagttc gggatttggc tcggatgggc tgtggtaaaa ggcatgttta ctgtgacaga
      961 ttcgattcaa aatatgattc cagatgtgct ggatttgttc aatttcattg aaagtacagg
     1021 gctaaataat gtttatcaat cagccgtaaa tacaattgtc attggattaa tgatcctttc
     1081 attgatgttt gttggttaca aaatgattac tggaaaagga acagtggatt taaaatcggt
     1141 tggaatgaac gtagtgatgt ctatagcatt aatactttta atgccaacca tgatttctag
     1201 tggtattcaa tttgctaaaa cattttataa ggactctact acgattacaa attcagatga
     1261 cggagtcgct tggtcattga taaaacaagg agtaacagat ttagcatata ttaataaaac
     1321 agatcagtac tcaagcattg ataaaacgga ggatcgaaat aatttaacac gaaaaaattt
     1381 tcaacagacg gatctcacac aagtgttaac ggataaagtg attgataaat tggaaaaaga
     1441 aaatccaatt gcggacaatc ttcgttatga attagttgaa aattctaata atgaatttgt
     1501 tgctacaaag ttttctgaca acttcttatc aacattttct gatagcttga aatcaggcta
     1561 ttaccgatac caagcaaatc tatgggggat ttcaattggg ttgacagcgt tagcaatcgc
     1621 ctatgtgttc agcgcgtttg tgatcattac agcaatacta gaacttgcat ttaaacgtgt
     1681 gttaggtgtt cttgtttttg caacagatat tgaga
//
LOCUS       DACEOE010000287         1699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.298, whole genome shotgun sequence.
ACCESSION   DACEOE010000287 DACEOE010000000
VERSION     DACEOE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.298"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..70)
                     /locus_tag="IW774_002752"
     CDS             complement(<1..70)
                     /locus_tag="IW774_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1155070.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASG"
     gene            complement(73..972)
                     /locus_tag="IW774_002753"
     CDS             complement(73..972)
                     /locus_tag="IW774_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1155071.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKKLYNQEMPQLMKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            complement(988..1581)
                     /locus_tag="IW774_002754"
     CDS             complement(988..1581)
                     /locus_tag="IW774_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1155072.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
BASE COUNT          477 a          322 c          240 g          660 t
ORIGIN      
        1 taccacttgc agtataacgt aaatctggat ctttggttaa tcgtcctact aaagttacat
       61 tattaatcat ttttatctct ccatttcttg attcatttgt tgtgatctct taaaattttg
      121 ttttcgttct actttctctt gctctacaat tgattgcatc aaagaatcga cggaagttag
      181 tgtagattcg aaagaaatgt ctttggcaaa ataattttct tgttggtatc ttttccactt
      241 ttccctcaac ccctcatcct tcatcaattg aggcatttcc tgattataca attttttaaa
      301 cacagattga ttatttcttt ccttgaacgt tccttgaaca ctcttataca agtttttctc
      361 atcgatacgt tctagttttg gaatcatata caggtcataa aagtctttca tgcgcgaatt
      421 atcttttccg aaactaaacg tagcatgtaa tttttcagct agaatttgtt ccgtaggata
      481 agctggaata tgaatcgttt tatcttcaaa cattaatttg tgttcataca tcaatgccgc
      541 tggaataata gaatcacctg aagtaatatc taatttaaat ggtacacgtg cattctcaag
      601 ataagcaata aactttgctt gaaaacctgg gtaataatcg gcttccctcg tctctgttaa
      661 tccctttaat tcaaatcgaa ttccatcttt tgtgggatca gctgttaact cagaaaaaac
      721 agtttctaac ttttcttttg ttagtttcga atttcgataa gtcgtatcaa tatcaacagt
      781 tgaacggttt tctaacccgt attttgatcc gatcagaaat ccccctttta atacaaaatc
      841 atcatgataa ggcgattcaa caatacgttc taataatttt tccatcatat aatatctttg
      901 gacaatttgt ggatttaaat gtttctctgt tgcgaaattt ctcgtttttg ctttaaattg
      961 tgtcggtgtc attcaccttt actcctctca ataaagtggc aacatataca aagtcatttc
     1021 ttttggtgat tttaaaatat ccatgtatct tcttagctta tttgtatttc tactggtact
     1081 tgtcatatat tcttttaatg cttcttgttt tacctctacg cttgctttgt atcttggatt
     1141 ccacatatca cataacgttc gctctaaatc atagactaaa attttattgc cgtaaaagct
     1201 ttctgtttct gtgattccca ggtcaaaatt ttctttattc gtgtaatgcg gttgaatagc
     1261 atactcgtct aatttctcct ttgaaacatt gtatccttta ggaaacatca tattgatctt
     1321 tttagggata tacgttccga cttgaaaaag atcgagcgca gtctcatgag aaaaaatacc
     1381 tctggaatag atactttgag cgagatacaa ttcatccacg aacgcttctt ttaaggtata
     1441 gaaacctggt gctacctttt ctattttgtc cttttcggct aatcgatcta acgtcatagg
     1501 cgataagcct tcacttctag catccttcaa ggatagattc ccattatgtt tatcaaaaat
     1561 gcactggatt ttgtctttca ttgcttttcc cctaaagtta atataaaact aacataacta
     1621 tatcacgtta gttttatatt aacaagaaag atatttatcg atctaattca atttcttttt
     1681 gaatccgttg ctggttctt
//
LOCUS       DACEOE010000288         1695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.299, whole genome shotgun sequence.
ACCESSION   DACEOE010000288 DACEOE010000000
VERSION     DACEOE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.299"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            126..722
                     /locus_tag="IW774_002755"
     CDS             126..722
                     /locus_tag="IW774_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1155073.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            735..1634
                     /locus_tag="IW774_002756"
     CDS             735..1634
                     /locus_tag="IW774_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1155074.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
BASE COUNT          636 a          255 c          346 g          458 t
ORIGIN      
        1 aagaaccagc agaggattca aaaagaaatc gaattagatc gataaaatct tcctaactat
       61 tgtatacgta taaacttatg ttatactgtt tttataagtt tatacgtata caaaggaggg
      121 agaaaatgac aaaagcaatg gtatacaagc tgctagaaga atttaatgga actttggctt
      181 tgaaggatgc caaaaaagcg ggcatttctc ctgtaacgat caaacgtatg gtagatcgag
      241 gggagctgga tcgagagtat ccaggttttt tcaccttacc tggtcaattt cctgatgaat
      301 tgtttatggc gcaaaaaaaa tatgaacgcg gcattatttc acatattacc gcgttagatt
      361 tgtatgattt aacagatatg atccctagac agatcgatct gactgtacct tacggttatc
      421 acgtttctga aaaaggattg aaggaattcg cagttgagct acactataca aaagcggaat
      481 ggtatgaatt aggtaagatt gaaataaaaa gtcgctatgg gaatcctatt atagcttatg
      541 atccagaaag aactttatgt gacatttgga atccctggta taccgttgag gatgaaatca
      601 aagtaaaagc aatcaaaaac tatatggagt cggacagaaa aaatcttaga aaattgaatg
      661 agtatcgacg aattttacca acagacaaaa cgatgcgttc ttatattatg gctttaaatt
      721 aaaggagaag atacatgaca cctacgcagt taaaagcaaa agtgaagaat atttctagag
      781 aaaaagaagt tgatccgcag cttgttttaa gacattttat gatggaaaaa ttcttggaaa
      841 aaatctcaga gtctccttat cgtgaaaatt ttgtgttaaa aggcggcttc ttgattggct
      901 caaagtatgg cattgaaaat cgcacaacca aagatattga tacgacttta cgggaaatga
      961 aagtaaccaa agaaacatta acaacggttc taaacgaaat tttttcaacg ccgacgaaag
     1021 aagacattca atttgagatt caaggaatga aagaaacgag agaagccgat tactatccag
     1081 gatttagctt acgtgtgctg gcacatttgg aaaacatgcg gccagatttt aaggtagatg
     1141 tgactacagg tgactcgatc tatcctgcaa cgatcactca cagtcataaa ttgatgtttg
     1201 aggatcgaac gattcctttg gaatcctatc ctacagaaca aattattgct gaaaaattaa
     1261 gtgcaacctt tgatttttta acagataatt cgagaggaaa agatttttat gatctctata
     1321 ctattcctaa aatggagaag atcgatgaga agaccttgaa agatagtgtg agaaatacat
     1381 ttacacgtcg aggaaaaact gatccgttaa agatatacta tgaaaaggat atggatgtac
     1441 tgaaaaatag tcacgagcta aaagatacct ggaagaaata tcaaaaaagc aatccttatg
     1501 ctgcgtcgat cacgtacgat gagacaatgg attcgattag taatttgatg gaaaaagtga
     1561 ttcacgtcga agaaaaagag cgaaaactag cttatcaaag acaccaacag atgaatagag
     1621 aaatggagag ataaagatga ttaataatgt gacattagta ggacgcttaa ccaaagatcc
     1681 tgatttacgt tatac
//
LOCUS       DACEOE010000289         1669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.300, whole genome shotgun sequence.
ACCESSION   DACEOE010000289 DACEOE010000000
VERSION     DACEOE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.300"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            103..573
                     /locus_tag="IW774_002757"
     CDS             103..573
                     /locus_tag="IW774_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1155075.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            658..1266
                     /locus_tag="IW774_002758"
     CDS             658..1266
                     /locus_tag="IW774_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1155076.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1400..1600
                     /locus_tag="IW774_002759"
     CDS             1400..1600
                     /locus_tag="IW774_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1155077.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT          555 a          240 c          340 g          534 t
ORIGIN      
        1 aaaggcaaag gatcaaaaaa aattaatttt tttaaataaa gatgaaagtt attgaaatat
       61 agagtatgat aaataagaat ttttaataag gtaggttggc tcatgaagca gtatcgacag
      121 ttaaaaggat atttagaagc aaaaggatat tgggagatac tagtctatct cttttttggt
      181 ggtttggcta cgattgtaaa ttttgttagt tttgctctcg cacgccaata ttttgattta
      241 agtatggcct tatccaattc tatttcgtgg ttttgttctg tattgtttgc ttttgtaacg
      301 aacaaacttt gggtattcca ttcaaaatca ccgaatttta cacatgcact tattgaatgc
      361 ggtaagttct tcttctaccg tatcctttct tacggattgg acatgggggc aatggtattg
      421 ttgatcaatg ttatgaacag caatgagtat gtggcgaaaa tcatcactca aattattgtt
      481 atccttgcga actatatttt cagcaagttg tttattttta aagaaactga ggtttttgaa
      541 gaagaagtag gtcaaagtga aaaagacgac taggcataac aattgaagaa tttccttctc
      601 tttgttactc tttgtaatgt agacattatt aagaaaacat tgaggaggaa tttttttatg
      661 acttacacat taccagatct accttatgca tatgacgcat tggaacctta tattgacgaa
      721 gaaacgatgc atctgcatca tgataagcat cacaatactt atgtgacgaa tttaaattca
      781 gcaattgaga aatacccaga attaggcgaa aaaacaatag aagaattatt atctgatatg
      841 gacgctattc caacagatat caagacagct gtacgtaaca atggtggcgg acatgctaac
      901 cattcatttt tctgggaaat catggcacca aatgctggtg gcgaacctac aggagaaata
      961 aaagaagcga ttaatgaagc ttttggtgat ttttcttctt ttaaagaaga attcaaaaaa
     1021 gcagccgctg gacgatttgg ttctggatgg gcttggcttg taatggaaaa tggaaaatta
     1081 gctattacct ctactgcaaa tcaagattct ccattgatgg aaggaaagac accaattcta
     1141 ggtttggacg tttgggaaca tgcttattat ttgaaatata aaaatgtccg accagattat
     1201 attgcagcat tctggaatgt tattaattgg gatgaagtaa acaaacgttt cgaagcagca
     1261 aaataaccat ttcctccgtc atatcagtct taaaaataat ttggaaaata aaagagcgaa
     1321 agcttgctag cgttttctaa attgggtata atgttcttgc aaggttactt gcaagaactt
     1381 ttttggagga tatacattta tggaacacgg aacggttaaa tggttcaaca atgaaaaagg
     1441 atttgggttt attacagtag acggcggaga tgacgtattt gttcatttct cagcaattca
     1501 aggagacggc ttcaagagcc tagaagaagg tcaagaagta gaattcacga ttgttgaagg
     1561 tgcacgcgga cctcaagctg ctgaagtgac taaattataa taatttgttg aataaagaca
     1621 cgacttctta gagagtcgtg tctttttgtc tatttatgaa gtttttaga
//
LOCUS       DACEOE010000290         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.301, whole genome shotgun sequence.
ACCESSION   DACEOE010000290 DACEOE010000000
VERSION     DACEOE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.301"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..505
                     /locus_tag="IW774_002760"
     CDS             131..505
                     /locus_tag="IW774_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1155078.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IW774_002761"
     CDS             complement(619..1248)
                     /locus_tag="IW774_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1155079.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDIA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IW774_002762"
     tRNA            complement(1382..1454)
                     /locus_tag="IW774_002762"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          585 a          240 c          282 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca atgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACEOE010000291         1636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.302, whole genome shotgun sequence.
ACCESSION   DACEOE010000291 DACEOE010000000
VERSION     DACEOE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.302"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            146..1630
                     /gene="guaB"
                     /locus_tag="IW774_002763"
     CDS             146..1630
                     /gene="guaB"
                     /locus_tag="IW774_002763"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1155080.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          563 a          284 c          378 g          411 t
ORIGIN      
        1 acaatctctt ttaatataca cagatgtaaa cgctttatat tggtattcta tgagaaagct
       61 aacttgattc caagtagatc attgacttaa atgaaaaaat caattatttt aatagataaa
      121 ctaaattaga ggagtggtac taaaaatgtc caactgggaa acaaagttca cgaaaaaagg
      181 atttactttt gatgatgtct tattgatacc agcagaaagt cacgtgttac caaacgaagt
      241 ggatatgagc gtacaattag ctaaaaatat taagttgaat attccaatca tcagtgcaag
      301 tatggatact gttaccgaca gcaagatggc cattgctatg gcaagacaag gcggactagg
      361 tgtgatccat aaaaacatga cgatcagcca gcaagcggat gaagtaagaa aagtcaaacg
      421 ttctgaaagt ggcgtaatca ttgatccgtt cttcttaacg ccacagcatc ttgtagcaga
      481 cgcagaagaa ttgatgagca aatatcgtat cagtggtgtg ccaatcgttg aaacacttga
      541 aaacagaaaa ttggtaggga taattacaaa ccgcgacatg cgttttgtaa cagattatca
      601 catgccgatt gctgatgtga tgacaaaaga caacttagtg actgccccag taggtacctc
      661 tctaaaagat gcagaaaaga ttttgcaaaa acataaaatc gaaaaattac caatcgtaga
      721 taacgaaggt cgtttaagcg gattgatcac gatcaaagac atcgagaaag tgatcgaatt
      781 tccaaatgca gcaaaagatg aacatggtcg attgcttgta gcagcagccg ttggtgtaac
      841 aagcgataca tttgagcgtg cgaatgcttt attagaagca ggggcagatg caatcatcat
      901 cgatacagct catggacata gtgcaggtgt gatccgaaaa attcaagaaa tccgttcaac
      961 ttttgcagat gcaacattga ttgcaggaaa cgttgcgacg gctgaagcga caaaagcttt
     1021 atatgatgca ggtgtagatg tcgtaaaagt tggtatcgga ccaggatcga tttgtacgac
     1081 acgtgtggta gccggtgtag gtgtacctca gctcacagct atttatgatg cagcatcagt
     1141 tgctcgtcaa tatggcaaag cgattattgc agacggaggg atcaaatatt ctggtgacat
     1201 cgtcaaagca ttagctgccg gcggacatgc agtgatgcta ggaagtatgt tggcagggac
     1261 agatgaatca ccaggtgaat tcgaaatctt ccaaggacgt cgattcaaaa cataccgagg
     1321 tatgggctca ttaggcgcga tggaaaaagg ttcaaaagac cgttacttcc aaggtagtgt
     1381 caatgaagca aataaattag taccagaagg aatcgaaggt cgggttgctt ataaaggaag
     1441 tgttgcagat attattttcc aaatgatcgg cggtttgcgt tcaggaatgg gttatgttgg
     1501 ggcagccaat ctacagcaat tacgtgatga agcgcaattt atccaaatga gcggcagtgg
     1561 attaaaagaa tcacaccctc atgatgtaca aatcacaaaa gaagcaccaa actattcagt
     1621 tgaatcttaa aaacat
//
LOCUS       DACEOE010000292         1621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.303, whole genome shotgun sequence.
ACCESSION   DACEOE010000292 DACEOE010000000
VERSION     DACEOE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.303"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(141..1433)
                     /locus_tag="IW774_002764"
     CDS             complement(141..1433)
                     /locus_tag="IW774_002764"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1155081.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          463 a          342 c          286 g          530 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctgaacca ttgtatcaat aatagataca tatttcaaat
     1501 aaaatcgaat gatttttatt tttattttat aaaaacgctt aaaaaacgaa ctttatcttt
     1561 ttagaatggt tattaaaaaa gaggttgtaa cagaagtggt cagcttcaag aaataagaag
     1621 g
//
LOCUS       DACEOE010000293         1616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.304, whole genome shotgun sequence.
ACCESSION   DACEOE010000293 DACEOE010000000
VERSION     DACEOE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.304"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..267
                     /locus_tag="IW774_002765"
     CDS             <1..267
                     /locus_tag="IW774_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ1155082.1"
                     /translation="NDEQSLEILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYD
                     QLSAKQKKMTKVLINAEGKLSHPLSIHVRNEWQLADSQLLFQEK"
     gene            413..706
                     /locus_tag="IW774_002766"
     CDS             413..706
                     /locus_tag="IW774_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155083.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            699..995
                     /locus_tag="IW774_002767"
     CDS             699..995
                     /locus_tag="IW774_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1155084.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            1070..>1616
                     /locus_tag="IW774_002768"
     CDS             1070..>1616
                     /locus_tag="IW774_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1155085.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQ"
BASE COUNT          601 a          218 c          323 g          474 t
ORIGIN      
        1 aatgatgaac aatctttgga aatattaacg actgttttta cagaaaagaa aatcaatacc
       61 attatttttg cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcaaag
      121 actatttacg atcaattgag tgcgaaacaa aagaagatga ccaaagtatt gataaatgca
      181 gaaggtaagt tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat
      241 agtcaattat tatttcaaga aaaataacat tttattttct catgttactt ataagtagtt
      301 ttacatgctt tttgacttta aaaatacatg tgatgataca tgttatatga attatcaatg
      361 tacccagtaa tatgctattt tcattatgta aaaaataaca gggggtgtgt agatggagat
      421 tgatgaaact tgtttgagta actcttataa aagtatgaat gacttttttg aggaaatgga
      481 actggattca gaattgctct atcaatttta tcttgctttt cgagggcaac agatttcttt
      541 tccaatgaaa atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca
      601 ggaaaaaact gtagatatcc gtcaattgac agaagtttat ggtttctctg caagatggat
      661 tcaaaaagta attaaacaaa aagaaaggag acgagtacat ggctaagctg attgtattag
      721 gtggtttaat ttacggtgga tattggttat ataaatatat gcttgcgcat ccgaatatag
      781 ataaaggaaa agtaattaag acagttgggg taatcatagt tggactcata gctctaacta
      841 agaaaaataa tactaatagc ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa
      901 gggaaatttc tactagtttt aatccatctg agagtcctca aggaagagac cgctgccata
      961 cgggctatcg acattattgg gaaagaatcg attaaaagat taccatttag atcaattaat
     1021 tgtctgatag aataagtacg tgagggagaa attgaaaggg ggaaatataa tgaaaaaatg
     1081 gctgttatta ctaataggaa tgggaatttt attcactggt tgtacatctg agaatacgag
     1141 gactaaagcg actatagaaa ctgatacaac aattgagact aaaaaaagta aaacgcaatc
     1201 aactgaacaa actacaacag aaatgaagca atctgtggaa acaagtgcaa gttcaatcac
     1261 tgaatcttct tctgaagagg ttaagaatac tttgtgggat acgaataaag cttccaaatt
     1321 agaaactttt gtcacacaat ggggaaagac attaggtcaa gaatataaaa gctacaatct
     1381 tcagaataat gtaagtctgt atggcactcc tttaccacaa gccgtgatta atggcgattg
     1441 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     1501 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     1561 gcatgtttac tttttcggtt ttcaaaatgg acaacctaaa gtttatttaa cgcaac
//
LOCUS       DACEOE010000294         1610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.305, whole genome shotgun sequence.
ACCESSION   DACEOE010000294 DACEOE010000000
VERSION     DACEOE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.305"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..431
                     /locus_tag="IW774_002769"
     CDS             <1..431
                     /locus_tag="IW774_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1155086.1"
                     /translation="YYLKEALSYSSQKERIHVSDTEIQLISCIGKIYGVAGKTAEARY
                     YLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEIDEALEQVNQGIYWAQEKNSY
                     YYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISKNL"
     gene            507..>1610
                     /gene="nagA"
                     /locus_tag="IW774_002770"
     CDS             507..>1610
                     /gene="nagA"
                     /locus_tag="IW774_002770"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1155087.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMT"
BASE COUNT          559 a          262 c          357 g          432 t
ORIGIN      
        1 tttattattt gaaagaagca ttaagctact ctagccaaaa agaaagaatc catgtttctg
       61 acacagaaat acaactgatt agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg
      121 ctgaagcacg ctactatttg agtcgaagca tccaactgct gcatcaatca gctgatgaac
      181 gatcaaaaag tgaattttcg caaatttttt ataattatgg aaatttctta ttccatcaaa
      241 atgaaatcga cgaagcgtta gaacaggtga atcaaggaat ctattgggct caagagaaga
      301 atagctacta ttacctcaat gatttatttg tgcttaaaag cttaatatac aagcgtaaag
      361 aagaaccaga aagagcagca ttctatgaag gcttagcaca agctgtgaaa caaatttcaa
      421 aaaatttgta aatggtgtag accattcttg acttcttctc taaataagag tactttagag
      481 gtagaaaaac aaagggagag aatagaatga gaacttttat ctatgctgac aaattctttt
      541 tagcgtcagg tgtcaaacat gcaggctatc ttgaaattac cgatggactt tttggtgaat
      601 acaccaaaga actacctgag ggagaagtca acatcattga ccaaagcggc aaatggattg
      661 ctcctggttt ggtagataca catattcacg gctatatgaa tcatgatgta atggataatg
      721 atgcagaagg catcaaagtg atgtctgaag gtctgttatc ttgtggagtg acttcatttt
      781 taccaacaac attgacgtca agcaaagaac gtttaaaaga tgtagctgag acaatcggca
      841 aaatgtatca ggaagcacca ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct
      901 tcacagaaga acacaaaggg gcacaaaacc caagttattt cggggatcct gatttggata
      961 ctttccatga atggcaagaa gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg
     1021 aacgtcaagg tgtagaagag tttgtcaaac aagtgactga agaaggagta gttgtttctc
     1081 taggacacag taatgctaca ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg
     1141 tatttgtcca tgctttcaat ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg
     1201 gtgcgttatt atctttagaa catgtctttt ctgaattgat ttgtgatggc catcatgtac
     1261 atccaaatgc tgcgggaatc ctaatggaaa aagcaggaca tgatcatgtc gcattaatta
     1321 cagactgtat gatggccggt gggatgcctg atggaaatta caatttaggc gaatttccag
     1381 tagtagtaaa agaaggaaca gcacgtctag aatctggcaa cttagctgga agtatattga
     1441 aattgaaaga agcaattaaa aatgttgtag actggggatt agcaacacca gaacaagcag
     1501 tcatgatggc tagtctgatt ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa
     1561 tcgctcaagg acgcgatgca gactttatcg tattgaaccc tgatatgaca
//
LOCUS       DACEOE010000295         1595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.306, whole genome shotgun sequence.
ACCESSION   DACEOE010000295 DACEOE010000000
VERSION     DACEOE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.306"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            291..1226
                     /locus_tag="IW774_002771"
     CDS             291..1226
                     /locus_tag="IW774_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ1155088.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            1270..1539
                     /locus_tag="IW774_002772"
     CDS             1270..1539
                     /locus_tag="IW774_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155089.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
BASE COUNT          560 a          220 c          314 g          501 t
ORIGIN      
        1 agaagtgaat tatctaacac aacaatatct gagttgaaga atggtagaaa gagtttagat
       61 accttaaaat tcagatcatt tgaagctcta tatatagctg ctaaaaaata ttttacagct
      121 gataataaat aaaaaattaa ggtgggttct atgtgaaaaa aacgtatcaa aagtttttat
      181 ggattagcta tatcgtccta tttatcgttg ggtttctcgt cagtgtgtcc gtgatcgtcg
      241 gaacgctttt tcttttgttc tctgacgaag atctggtact taaggggtta atgagtacgt
      301 tatcgtgtgt catgttactt tatttatgta tacagatcaa cgaaattatg ccccctcatt
      361 ggcaagaaaa gaagaaagaa tttaaggaag aaatcgaaat aaaatccaat gactggattg
      421 aatacacatg gcttcctctg aaaagaaata gcaaacgaaa ggaattttgg gcattgcaat
      481 tatggccaat aggaaaactt tttatttata tggcttcact tatggttact ttaatcggtt
      541 atttggtggt ggtctatggg gtggcctatt acttgttaag tgagccaaaa cagatcgcgg
      601 cgtcaaatat tttcacggca gtaatgacat taatgtgtgt catgtgcttt tctattttta
      661 gttacttact cgtccaattt atcaggcaaa atttaacaat gagtaaactt ttcaaaagtt
      721 tattggactt acttatctta ggcagtatgt taacgactct aacagatcgg ttcgaacaat
      781 ctgaaaaaac ggtagagggg ataaagaact tcttaacgac gattgatcaa tttataaatg
      841 gatatatgta tttgtttgaa gtaggcttta ttctcacttt gtttggttgt gtatttataa
      901 aactcattaa aagatcaatc gaaaaagaaa tggttattga tctaattccg acgctgagcg
      961 gctatgagta ttacgttgat tttggaaagt atgaggagtt gatccatacg gatatcgttc
     1021 acacgagtat ccatgaagta accgttaagg gcatcagatc tactttaaaa gatggacagg
     1081 agacgaaaac aatcgaccga agaaaagagg tctacaattt ttataaagtt gaagcaagtc
     1141 tacgatatgg cattgggaaa ttgaggttag atgggagtaa tattcaacaa aacaaaccat
     1201 atgttttagg aaaagtggtg atttagaact tcctatgaaa gatgaataaa taaggagaat
     1261 cttttgaaaa tgattcttct ttacagaatt gtgcttatag ttggcacgga gttaaaaatc
     1321 ttaaagaagg agcagttatt caaagtgaaa gaacatcata atcagttact atgtattaaa
     1381 acgggtacga tgaacattca ataccgaaca gaatatttag aattaacgaa tcaaactgat
     1441 tttttgtcga ttcgagagca acataagcaa gaaaaattaa tggctactta tagccaattg
     1501 attgatgagt gggaacaatt caaggaatct aaaacataaa attccattta aacaccttat
     1561 tttttaaaga gcataaggaa acgtgacccc acttt
//
LOCUS       DACEOE010000296         1586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.307, whole genome shotgun sequence.
ACCESSION   DACEOE010000296 DACEOE010000000
VERSION     DACEOE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.307"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            76..1542
                     /locus_tag="IW774_002773"
     CDS             76..1542
                     /locus_tag="IW774_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1155090.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          441 a          291 c          332 g          522 t
ORIGIN      
        1 ataagttttt tagttaaatt tttcggtaaa gatgtataat attttctgaa aatttaaaga
       61 attggggtat agattatgga aaaacagcaa gacaagggct tcttcgggca gccgaaggga
      121 ttgtcaactt tgttcttcac tgagatgtgg gaacgattta gttattacgg tatgcgtgca
      181 ttacttatct attatatgta cgatacagtt gccaacgggg gacttggatt accacgagca
      241 acagcattag ctatcatgtc gatctacgga tctttgatct acatgtctag tatcgtcggc
      301 ggatgggtat ctgaccgagt gttaggagca aaacgcactg tattcttcgg tggagttttc
      361 attatgcttg gccacatcgt cttggcttta ccatttggtg taagtacatt gtttgtttcg
      421 atggcattga ttattatcgg aaccgccttt ttgaaaccaa atgtttctgg catggtcgga
      481 catctttatt caaagactga cttgcgaaga gatgcaggat tttcgatttt ttacatgggt
      541 gtcaacttag gggcattatt agcaccacta atcgttggta caatcggtca agaatataac
      601 tatcatttag ggttctcttt agctgctatt gggatgttct ttggattatt acaatacatc
      661 atccaaggac gcaagacatt agacggagtg ggtacaacac cgccgaatcc tttgacaact
      721 gaagaacgta aaggattcat ccgtaacttg atcatcgcac ttgttgttat cgctgcagta
      781 ttcggcgggg cacaactaac tggtcatctg acaattgact tttttgtcaa tacaatcagt
      841 gtattgggga ttatcttacc gttgtattac tttatcaaaa tgctgacttc aaaagatgta
      901 actgctgaag aaaaaccaag agtatgggct tatatcccat tattccttgc agcaatcatt
      961 ttctggtcat tacaagaaca aggttcatct atcttagctt tatttgctag tgaacgtaca
     1021 caatctacgc tatttggtgt aacgatccaa gcaagctggt atcaatcatt gaatccaatt
     1081 ttcatcttta ttctgacacc agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa
     1141 ccatctacag tagtgaaatt ctctcttgga cttttatttg caggattatc gtacttgcta
     1201 ttgatgttgc cgggtatgct ttatggtaca agtggaaaag taagtccatt gtggttgttg
     1261 ggaagtttct tcatcgtggt tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg
     1321 acaacaaaat tagcaccacg agcatttgaa tcacaaacaa tcgctatttg gttcttagct
     1381 gatgcttctt cacaagcaat caatgcacaa attgcacgct tctacacacc gggaacagaa
     1441 tctgcttact tcggtattat cggtgcagta gcaatcgtcg gtggtatctt gctatttact
     1501 gtgaaagaac caatcaaaaa actaatgggc tctattcatt aatcaatgaa cataaaaaca
     1561 ggaattgctt atttgcagca gttcct
//
LOCUS       DACEOE010000297         1581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.308, whole genome shotgun sequence.
ACCESSION   DACEOE010000297 DACEOE010000000
VERSION     DACEOE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.308"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..742
                     /locus_tag="IW774_002774"
     CDS             200..742
                     /locus_tag="IW774_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155091.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="IW774_002775"
     CDS             898..1347
                     /locus_tag="IW774_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ1155092.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
     gene            complement(1377..>1581)
                     /locus_tag="IW774_002776"
     CDS             complement(1377..>1581)
                     /locus_tag="IW774_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1155093.1"
                     /translation="KVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQI
                     RQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          600 a          217 c          308 g          456 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tccgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt g
//
LOCUS       DACEOE010000298         1575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.310, whole genome shotgun sequence.
ACCESSION   DACEOE010000298 DACEOE010000000
VERSION     DACEOE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.310"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..381
                     /locus_tag="IW774_002777"
     CDS             <1..381
                     /locus_tag="IW774_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HAQ1155094.1"
                     /translation="YYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVEN
                     NHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFC
                     LTGITAWNTFVELKKKRRRTQKFL"
     gene            509..1192
                     /locus_tag="IW774_002778"
     CDS             509..1192
                     /locus_tag="IW774_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1155095.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1501..1574
                     /locus_tag="IW774_002779"
     tRNA            1501..1574
                     /locus_tag="IW774_002779"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1535..1537,aa:Arg,seq:tct)
BASE COUNT          521 a          258 c          336 g          460 t
ORIGIN      
        1 tattatgcac aaggaaatct gatatatgta cgaccgaaaa atacaaatga gattttagtc
       61 ggagatatta ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata
      121 gccgttgaaa acaaccacga agatttttat acaaaagctg atacaaagac ttatattgag
      181 cctggagtag taaaaaagag aaatattatc ggtagtccca tctttcagat tccatatatt
      241 ggactagtcc tttcaaaaac ggcatttgca tggattcagg ggttattctt tacgattgct
      301 ttttgcctaa caggaatcac agcatggaat acctttgtag agttgaaaaa gaagcggaga
      361 cgaacacaga aatttttgta agaatgttaa aaacataaat gtattccaga gatccaacga
      421 ctcttctgaa catgaaacac gagaaaacac tcatgcagct tgtagattta gtcagttgtt
      481 cgtataatgt gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt
      541 caaagaagaa ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg
      601 caaactggaa gaagaagctc atgtagcaga agtaccagtt attccccatg aaacagttgt
      661 atttttgaaa tttttacttg gacagcttca gcctgaacga atcttggaga tcggagcagc
      721 tatcggtttt tcatcaagtg tcatggccac gacaatcgga gaaaacgctc atgttacgac
      781 aattgatcgt ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac gtctaggact
      841 gacagataaa gtgactttgc ttgaaggtca agcagcagat atcttgccgg agttgtctgg
      901 tccgtatgac tttattttta tggatagtgc aaaatcaaaa tatattgaat ttttacctga
      961 atgcttacgt ttgttaagaa aaggtggcgt tttgatggta gatgacatct tccaaggagg
     1021 aacgatcttg ctcccagatg aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt
     1081 aaatgaattt cttcgtgttg tgatggatca tcccgatttg acatcgacaa tcttgccatt
     1141 aggtgatggt gtgatcttaa tgacaaagga atcagaaaca atcgagttgt gatacatgat
     1201 tatggcagcc tgtagataat cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt
     1261 ttctatattt gtaagtcaat taaaggatac aggaaatcta aataaacagg agtagcatga
     1321 agtcaatgta gctcctgttt tcaattgata accttagaac agctgaagga gaatgtctcg
     1381 ttttaaaaga ttgatttagt agttgggcca aaataattca ttatttgcta attaaataac
     1441 tatttcgtat tgacagaagc aatttaatta tctataatta ttgatgttcc gctgattctt
     1501 gtcatagtag ctcagctgga tagagcatcc gccttctaag cggacggtcg ggggttcgaa
     1561 tccctcctgt gacgc
//
LOCUS       DACEOE010000299         1560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.311, whole genome shotgun sequence.
ACCESSION   DACEOE010000299 DACEOE010000000
VERSION     DACEOE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.311"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..476
                     /locus_tag="IW774_002780"
                     /pseudo
     CDS             1..476
                     /locus_tag="IW774_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1094)
                     /locus_tag="IW774_002781"
     CDS             complement(378..1094)
                     /locus_tag="IW774_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1155096.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1144..1353)
                     /locus_tag="IW774_002782"
     CDS             complement(1144..1353)
                     /locus_tag="IW774_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155097.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
BASE COUNT          472 a          372 c          289 g          427 t
ORIGIN      
        1 ttattttttg atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt
       61 attaaaggga gattggatga ctcaccttcc cattgtgaac actgtggatc gattcgtatt
      121 atccgcaacg gttcctatac gacacgtact cagatgttga aagtcaaaga aaagttgact
      181 attttagaat taaaacgtac ccgttttcta tgctatgatt gtgggcagac cttttctgcg
      241 aaaacggact tagttgatga acatcatcaa ctaacaaagg aactgaagca ggcgattctc
      301 atagaactgt acgaaaatca atcacgtaat ctgaaaatca gaagaagtac cgtcggctca
      361 agcgatattg gaaactctta ctcaaagatt ccacgacatt ggagccgctc aaacggcatt
      421 atcatcgact gtttaaacga ccaattagtc aaacagaaat cgtcgatgaa ccgttgtgca
      481 caatggtgtc ctgaacgtac agcgacacct tccatatcca gagcagtcgc cacatcacgg
      541 ggatggatgc catccaaatt aaaagcaacc acgccagtat gatgttcagg atcttgcggt
      601 ccgtaaatgg tgataccgcc gatcgcttgt aatttaggta aaacgtaggc aaccagttct
      661 tgctcataac gatgaatatt ctccatacca atctcattca agtaatcgat tgctcctgca
      721 atgttcggag taccagcttc aaatttccat ggcagctctt tccatgaact atcgtaaaga
      781 ttgacaaaat ctatcatttc accgccgaat tcgactggct ccatttgttc taacaattga
      841 cgtttgccat ataatacacc gattcctgtc ggcccgcaca ttttatgccc gctaaaggcg
      901 aaaaaatctg catccaacgc ttgtacatca actgtcatat gcggtgctgc ttgggcaccg
      961 actggactat cctcaactac aacagcctgt tcaaccctca gccgtttcaa gcacaacaaa
     1021 taaccttctg gatctggttt gccgacctga agatcatcga tcgtcacaaa ggaatcaatt
     1081 ttttccttaa tcatcggatg tgcttcaaaa aaatagtcta gctcccgcag ttcagaattg
     1141 agactactcc gattttccag tgctgctgat ccgctgctca aaatgttcag catccactgg
     1201 tctcccaacc gcttgactta tcccttcata aaaggacagt tctgctgccg tcccaacacc
     1261 ttgttgatag tctgcaaaag gaattttata gccgaactgc tccagtaaat aggcttcagt
     1321 aaatagtgat gaatactctc gtaaaacatc cattcactgt caatcatcgt cccatcaaaa
     1381 tcaaaaataa gtgctttggt catcttcttt cccccttacc caaaacaagc aaaagctgtg
     1441 tcctgctctc tctacagtat cagcataaaa actgccgtct gaaaagtcaa aggtgaaaga
     1501 aatgtggcgc tataatttct aggactgata gtaaatcatt ctatcagtcc ttttgagcat
//
LOCUS       DACEOE010000300         1545 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.312, whole genome shotgun sequence.
ACCESSION   DACEOE010000300 DACEOE010000000
VERSION     DACEOE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.312"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..190)
                     /locus_tag="IW774_002783"
     CDS             complement(<1..190)
                     /locus_tag="IW774_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1155098.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMAL"
     gene            334..1107
                     /locus_tag="IW774_002784"
     CDS             334..1107
                     /locus_tag="IW774_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1155099.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1278..>1545
                     /gene="ssrA"
                     /locus_tag="IW774_002785"
     tmRNA           1278..>1545
                     /gene="ssrA"
                     /locus_tag="IW774_002785"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          497 a          297 c          288 g          463 t
ORIGIN      
        1 tcaatgccat cagtaagccc ataagaaaaa accagtttcc gcttgtcttt tgagccagcc
       61 gccaatgagc gtctgttttt gcggcaagag gcgaatgata cccataaaaa ggcagatttc
      121 cttttgaggg cagaatccga aaaagactgc caattattaa gaataagata ccaatactga
      181 aaaaaatcat tctttccctc ctaaattctc ttcttatagt atactctaaa ttgttgactg
      241 aaccaatatg ttcaggaatt cttcacgaaa ggtttgtctt ttgttcattc tttgttaaga
      301 taaacttatt ccaataaaaa aggagtttta tttgtgtcaa attatcgtgc aatcactttc
      361 tttgatcttg atggaacatt actagatgcg aaatcaaaaa tcacacccga agtggcaaaa
      421 gctatgaacg cattaaaaga aaacaatgtt ctccccgtca tcgctacagg acgtacagaa
      481 gcagaaatcc actctatcat ggaagatgcg ggcattactt ctgcggtcac catgaatggt
      541 tctttcattc gagtagacgg cgaagaaatc tattctcaaa cattttcttc agaagaatgt
      601 caaaaaatgt atgatcatgt acagttgaac ggtcatgaac tttcttacta taacgatcag
      661 aaaatctggt gtaccggtca tagccaaaca atgatcaatg cttacgactt cattcattca
      721 gatgttccgg aaattgatcc tttaggcttt aaagaaaaca cagtaaatat gttgctgatc
      781 ttatctcaat caggtgacga atactatcac gaacattttc ctgagctaac tttttatcgg
      841 aatggtcctt actcgatcga tacagtgaag aaaggaatct caaaaggggc aggtgtcaaa
      901 cgtctgcaaa aagaacttca gctggaaaaa gttcctactt ttggatttgg cgatggtccg
      961 aatgattttg ccttgttgga agcttgcgac aacaaaatcg ctatgggcaa cgcctatgac
     1021 ggattaaaag aactttctac ctttatcaca aagaaaaata cagaaggcgg aattgtccac
     1081 gcattgaagt attttgatct gatttaagat ctttttttga agctacctct tttcttaaga
     1141 aaatcgctta ttttttctta agaaaggggc agttattata taaataatgg gatatgattc
     1201 gcttttttat tgaatttatc ccaatatatg ctatactaat tttgtatgaa aaatacacgt
     1261 aacccttgtt agctaacggg gacgttacgg attcgacagg cacagtcgag cttgaattgc
     1321 gtttcgtagg ttacgtctac gtaaaaacgt tacagttaaa tataactgct aaaaacgaaa
     1381 acaactctta cgctttagct gcctaaaaac agttagcgta gatcctctcg gcatcgccca
     1441 tgtgctcgag taagggtcct aactttagtg ggatacgttt caactttccg tctgtaagtt
     1501 gaaaaagaga acatcagact agcgatacag aatgcctgtc actcg
//
LOCUS       DACEOE010000301         1537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.314, whole genome shotgun sequence.
ACCESSION   DACEOE010000301 DACEOE010000000
VERSION     DACEOE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.314"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..365
                     /locus_tag="IW774_002786"
     CDS             <1..365
                     /locus_tag="IW774_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155100.1"
                     /translation="QGSGFVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSC
                     LLVFTSMFSLLPRLIIGPNGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYF
                     WTEIRIKSERSVSIKNNY"
     gene            562..1452
                     /gene="pstS"
                     /locus_tag="IW774_002787"
     CDS             562..1452
                     /gene="pstS"
                     /locus_tag="IW774_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1155101.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          519 a          271 c          316 g          431 t
ORIGIN      
        1 tccaaggaag cggatttgtg gtttctagga attttccagc tttgattgtt ttctatttag
       61 tgataagtgt aggtctctta ttaccaaaat ctctgatcaa tcactacaat tggcagctgc
      121 tttacttttc ttgcttatta gtatttacat ctatgttttc tttgctgcct cgcttgatta
      181 tcggccctaa tggtttgagc gagagtggat tgaaggattt agcttattcc tccatgtatt
      241 tcttgacgat caaaccatgg ttctatttag taattgcctc atttattctt gcaacatttt
      301 tttacttctg gacagaaatc aggataaaaa gtgaacgatc tgtatctata aaaaataact
      361 attgatggtg gatcatcagt aaaaaaacat atgagctgcg taaaatgcat gtttctcata
      421 tgcttttttt attattatgt aaagaatgcg aaaaacgttt acatgatttt tacataactt
      481 aactatattt ttacagcaac catacgaggg atttacagtc gactgataaa ctttagttgt
      541 aaataagaaa ggagcaataa aatgaaaaaa gtattgttta ctctaggtat gatgggaatt
      601 cttttagccg gatgcggctc aggatcagga aatacagatg gctctgccac acaaaataat
      661 tcaagtgact caaatgaaca agtaaaaatc gttgcggtag gttcgacagc attacagccg
      721 ttagtcgatg cagcacaaga aagctttgta caagaaaatc caaattatca aatctctgta
      781 caaggaggag gaagcggaac tggtttgagc caagtcgaag ctggagcagt tactatcgga
      841 aactctgatg tatttgcaga agaaagagac ggagttgatg cttcaaaatt agtggatcat
      901 aaagtagcag tggtcgggat ggcaccaatc gtcaataaag atacagatgt caaagatatc
      961 accaaacagg aattgattga tatctttaca ggaaaaatca ctaactggaa ggaagttggc
     1021 gggaaagatc aaaaaatcaa cgtcgtgaac cgtgcaaacg gcagcggtac ccgcgcgaca
     1081 ttcgaaaaat ggggactgga tggtgctacc cctgtgcagt cccaagaaca agattcatca
     1141 ggaacagttc ggcagctggt aagtcaaaca ccaggagcaa tcagctattt agctttctca
     1201 tatctcgatg attccacaca agcactaagt atcgacggtg tagaaccaaa agaagagaat
     1261 gtagcagata acagctgggg aatctggtca tatgaacata tgtacacgaa cggaaaacct
     1321 tcccctgaag tccaaaaatt cttagactac atgatgacag aagaaatcca agaaggacct
     1381 gtcaaagaat tgggctattt accaatcaca atgatggaag tagaacgtga tcacgaagga
     1441 aatatcaaat aactagcaga taaacatgcc acggtagtcg ttactattaa ggaaaaatca
     1501 actatgtttc cccacatata catttttcct ttaattg
//
LOCUS       DACEOE010000302         1533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.315, whole genome shotgun sequence.
ACCESSION   DACEOE010000302 DACEOE010000000
VERSION     DACEOE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.315"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..775
                     /locus_tag="IW774_002788"
     CDS             <1..775
                     /locus_tag="IW774_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1155102.1"
                     /translation="LDRNGKIPEENIQALRKFDENGGIVTVATGRNSISAKDIFSELG
                     IGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAKISIIASRETQDD
                     QLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALTDKSEEKLQKLQR
                     DLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMAFGDQENDIDMLN
                     YSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            815..1312
                     /locus_tag="IW774_002789"
     CDS             815..1312
                     /locus_tag="IW774_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1155103.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT          538 a          269 c          292 g          434 t
ORIGIN      
        1 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
       61 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
      121 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
      181 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
      241 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
      301 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
      361 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
      421 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
      481 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
      541 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
      601 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgatatgt tgaattacag
      661 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
      721 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga
      781 ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa
      841 tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg
      901 tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga
      961 tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct
     1021 tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga
     1081 aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga
     1141 agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc
     1201 ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga
     1261 atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct
     1321 atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta
     1381 cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc
     1441 acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg
     1501 caaaatatga ggaactattt ttccgtttgt taa
//
LOCUS       DACEOE010000303         1530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.316, whole genome shotgun sequence.
ACCESSION   DACEOE010000303 DACEOE010000000
VERSION     DACEOE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.316"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(185..1168)
                     /locus_tag="IW774_002790"
     CDS             complement(185..1168)
                     /locus_tag="IW774_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ1155104.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT          470 a          279 c          231 g          550 t
ORIGIN      
        1 gcttatttaa gaagaagttg cttctgctcc caccgtttat tcgtttttaa agaatgagac
       61 aaaagtgttt cttacttttg tcccacgctc ttattcatgc tcagagcgcc aatagataca
      121 aaatcatagc caaagtttca cgaaccctag ctgtcaaaga atcgatattt tattttaata
      181 taggctattt taaaataaat ggtttaaggt attcctctac aaaagccact ctatcaatta
      241 tcatgggttt aaaataagaa gtgatggaga taaccagatt gttcttagcg tcaatataaa
      301 gaacatttcc actattacca attgctgaat atatttcgtt ctcagaatct aatacccacc
      361 ataaatagcc atatctcata ttttgtaagt cttctaaagc actggttcta ggtttgaaca
      421 tctcgtttat ccacatgggt ggtacgattt gttttccttt atacatccct ttatttaggc
      481 ataatacacc aatttttgcc agatcacttg ccgataaaca caaaccatat cctgccgtcc
      541 cgatatttag tggatcacaa aaccagacat tggttttagg agacttatcc attgtaaact
      601 gcttatgttc ttcagctgtt tcagcataat aattgacatg gtttggtatc cctataggat
      661 caaatagata cttattggca aaatcaacag ttgttagctt acttgtctga tacaacaagc
      721 cagttaatat gtgtaaacag acagtttgat agttgaattc gccggttata ccttttcttc
      781 ctcccaataa atctagtgat gtttttgtcc aatcttcact tgaacagact tttgtccatg
      841 gatcgccttt acatttgtag ggtgctttca ttgttagcaa gtgcttgatt gtaatagcgt
      901 aaatagtttt ctccccgcgt ttcactttat actctggaaa ataatctaat actttatcat
      961 cgatactttt gattaatcct tgctctaaag caatgccaat cagcaaagat acaatacttt
     1021 tcgttgctga catgatatga caagtatcgt cttttttata accattccat tcatctgtat
     1081 aaacttctac cccatcctta tatgcgacaa tttgacatat atttggttgt cgaaaaccaa
     1141 taaaatcatg caactcttgt ttattcataa gatattacct cactttcttc tataagcata
     1201 atgtcattaa aaatcgattt caattttttc gattcttcac gtaatacagg tattcttatg
     1261 gactagacag cgctttggga agatggattc tctgaaaaat ataaaggttc actttatgag
     1321 tggataaaaa aagcagcaaa tacttcgact tctttaattt accaacatta gtgaaaatcg
     1381 attcatctgg agatttgtaa tttagagtcc ttgtcttgat tagaggtgta taaagctttt
     1441 tattagacat ttttaaattc cattacaatt tgcttataaa ctaggttttt aaaggtgact
     1501 atggtaagat aatagagata ttattttgtg
//
LOCUS       DACEOE010000304         1527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.317, whole genome shotgun sequence.
ACCESSION   DACEOE010000304 DACEOE010000000
VERSION     DACEOE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.317"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(343..1383)
                     /locus_tag="IW774_002791"
     CDS             complement(343..1383)
                     /locus_tag="IW774_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1155105.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          470 a          237 c          207 g          613 t
ORIGIN      
        1 acaaaaaatg taagcgaata atattcgtat tgcctgaaaa gaagtaatat caagattttt
       61 agcttgttta aataatgaaa ttatctctct tcgcgtatta ttataactca aaaaaaagga
      121 gtatgcaaac atacgttccc ttttctatat ttgaaattaa ttatttcttt taacacttat
      181 gacgtaaaag gctacgtttg tgatttttat cataatattt taacttataa attctattaa
      241 aatagctatt attttgtaat attcgtaata taattagaat aataaaaaat ttttaatttt
      301 aatgttactc catgaagcaa caatcacttt gtcagtaaag tttcactcta accagtcttt
      361 cgtaatagtg cttggggagc tttcataatt caaacgtttt tcacttgata ctttagcagc
      421 tattggtttt tcgataaacc aattttttac atagacaaaa atcaggttct ggatattttc
      481 gactttttcg gtttttaatt tttgataggc ttttaacagt gtcgtgtata attcttgttc
      541 tgcattgaca taataactat cgagttcttc aaaaactatc gaaataccac tttctatttc
      601 agcttttttc tttgcattat gaatagtctg gacggttttt tgagcttcac tgtaacttga
      661 agaaaatgtt tttatcaaat ttaatactcg ttcagggaca aacgtagcaa tgctgtcgtt
      721 tgtcataatt gtctcaaaat tgtctaacaa caattgatct tgtaggcggt ctttttcagt
      781 gtctatattg tgtctattag tgtctaatat attattatta agttctggta tgattttcat
      841 accctctgat ttatcaacgt tttggcacgg tatgattttc ataccctctt gagccccaag
      901 ggattcagcg tctgattttt cgtcgagggt gggtatgatt ttcataccct ctgatttatc
      961 aacgttttcc ctattctctc tatcaaattt ttcgagtaag tagatatcat tttctgagac
     1021 ttttaattct gctagatata gtctattagg attatttttc cctaactgtt tgttaaatcc
     1081 catctgcttt tgtaataata aaccaaagga ttccaactct tttttgattg ctatcgcttt
     1141 ttgtttttgg cagtttaata ctctacatag ttcatttgtc gtataagtaa aataaacatt
     1201 accttcttca tcgatttgcc gtttgctgat tgcgatctct aatcttgctt ttaaaagcat
     1261 ataggcaatc ttagttgaat ctttcatttt tttgtattcg tcagaaacaa taaagacttt
     1321 tggaaattga taaaataatt cgttgtaaac tctatccgcc ttaaaaaatt taaaatcagt
     1381 cattgtagat cctctacttt cacgttgttt tatttatgta aaattaccta ctcttttcga
     1441 aaatagcaaa gataaataaa aaatgcaaac gcattaaaac tgttttttaa ttaaatacta
     1501 cctaaaatca gaaataaaat gaattat
//
LOCUS       DACEOE010000305         1527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.318, whole genome shotgun sequence.
ACCESSION   DACEOE010000305 DACEOE010000000
VERSION     DACEOE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.318"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..376
                     /locus_tag="IW774_002792"
     CDS             <1..376
                     /locus_tag="IW774_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1155106.1"
                     /translation="INPDFIETVNYVTNFQREMRKVEAMTVLATQSPQEMVPENMSSD
                     NFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKSMTNSELESIPQQNQGDAVINFGS
                     KETIRVHFSPNKRQLKLFDGGK"
     gene            435..698
                     /locus_tag="IW774_002793"
     CDS             435..698
                     /locus_tag="IW774_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component,
                     AbrB family protein"
                     /protein_id="HAQ1155107.1"
                     /translation="MLVKEIEMKKWGNSQGIRFGKEELSVLGETNTENLKFEMVVTEG
                     KIVLTPKNKKPETLDELFANYEGEPLGKEDKYEWGNMQGREII"
     gene            698..1033
                     /locus_tag="IW774_002794"
     CDS             698..1033
                     /locus_tag="IW774_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1155108.1"
                     /translation="MKKNYHDLSQGDLVYIDLNPTKGHEQRGKRPCIILSKPNKYLNY
                     MIGVAPITTKAKPFPVHVDLNGTQKIKGQILLEHHRMIDIESRGFQFVEAVPKEILEE
                     CIEKIKLFY"
     gene            1107..>1527
                     /locus_tag="IW774_002795"
     CDS             1107..>1527
                     /locus_tag="IW774_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HAQ1155109.1"
                     /translation="MENDTNIVMEHLKRRAKRRLFLWLFGTSAGLITILITVFVTLFL
                     ILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKL
                     LPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLF"
BASE COUNT          528 a          209 c          316 g          474 t
ORIGIN      
        1 gattaatcca gattttattg aaacggtaaa ttatgtcaca aactttcaac gagaaatgcg
       61 taaagtagaa gcaatgactg ttttagctac acaatcccca caagaaatgg tgccagaaaa
      121 tatgtcttct gataacttct cgaaactgaa gaaagtcttt gaattttctt ctacgaagat
      181 ctttatgcgt atggatgaat ctattttacg tcatatagaa ggattagttg gtaaatcaat
      241 gacgaactca gaactagaat ccattcctca acaaaatcaa ggagacgctg tgatcaattt
      301 tggctcaaaa gaaaccattc gtgttcattt tagtccgaac aaacgtcaat tgaaattgtt
      361 tgatggtgga aagtaacttc ttgtattttg gtgtatacaa gtatatacta atgtgtgaga
      421 gaaggaggga ttccatgtta gtaaaagaaa tcgaaatgaa aaaatgggga aattcacaag
      481 gtattcgttt tggaaaagaa gaattgtcag ttctaggaga aactaatact gaaaatttaa
      541 aattcgaaat ggtggtaaca gaaggaaaaa ttgttttaac tcctaaaaat aaaaagccag
      601 aaactttaga tgaattgttc gccaattatg aaggagaacc cttaggaaaa gaagacaaat
      661 atgaatgggg aaatatgcaa ggtagggaga tcatttaatg aaaaagaact atcatgattt
      721 atcgcaagga gatcttgttt atattgattt aaatcctacc aaaggacatg aacaacgggg
      781 aaaacgacca tgtattattt tatctaaacc taataaatat ttgaactata tgattggtgt
      841 cgcaccgata acaacaaaag cgaagccttt tcctgtacat gttgatttga atggtacgca
      901 aaaaataaaa ggacaaattc ttttggaaca ccatcgaatg attgacattg agagtagagg
      961 ttttcagttt gtggaagctg tgccaaagga aattttagaa gaatgtattg aaaaaattaa
     1021 attattttat taaaagcagc gaagaataat tattatttct tcgctttttt gttgtttaaa
     1081 attaaaaagt taggagtact gactatatgg aaaatgatac aaatatagtt atggaacatt
     1141 tgaagcgtcg tgcaaagcga cgcttatttt tgtggttgtt tggtacttct gcaggtttga
     1201 ttactatttt gatcactgtg ttcgtaaccc tttttctaat acttgcggcg ggttcgattg
     1261 ataattcgga ttccgattca agtagtggtg gcgaagcttt tacaggagag tattcggaag
     1321 gcttaccgat ttataaagaa atcaaaggaa gaggcccttt ttctgatgag attgcgcaat
     1381 atgccgtagg tgcagcagtg aaatataaat tattaccaag tgtgattctt tcgcaatacg
     1441 gttatgaatc agcgtttggt acgtcggcgt ccgcaagaaa tgatctcaac tattttggga
     1501 ttacttggtt tgatggttgt ctgtttc
//
LOCUS       DACEOE010000306         1508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.319, whole genome shotgun sequence.
ACCESSION   DACEOE010000306 DACEOE010000000
VERSION     DACEOE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.319"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            203..559
                     /locus_tag="IW774_002796"
     CDS             203..559
                     /locus_tag="IW774_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155110.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          544 a          187 c          276 g          501 t
ORIGIN      
        1 acattagatg aaaagagaca aaaagaattg cagcaactaa agcagaagta tggcaaaagt
       61 gaatctaaga ttatgtgtat tgcgttagat ctattgatcg cgcaagaaaa agcaggattt
      121 gatgtaccag cgctaaaaaa gtgacgtcac tttttaattt gatctatata agtgatatca
      181 cttttaccaa ggagggaatt aaatgttaaa aactaaaaag aattcgtaca aattaaagct
      241 tagtatacta tctattattt gtcttttagg catgataatt agtggtcatt tgtatagagt
      301 ggcatatacg cagtttattg agtacaaggt tcactcttta ggattagctg gggatcgata
      361 cgttgaatat gtccattatt cattaaaatg ttcttcatat attcttcttt ggcttatttt
      421 ttcattgttt gaattgctaa atttaatagc aatatcttca tatcacttag ataagctaaa
      481 taccaaatct agccgttata acgaagtgtt taaggaaaat gtgaagttag aaagtgactt
      541 aaacaagcta aaaaaataaa aagaagaaaa cataagttaa ctaaaattat tttattaaga
      601 atgatggaat ataaggataa ggttaccaac ataacgatca ttacttctct gatatgctat
      661 ggacttctat caatcagtat tttgtctata gattatggag taactatact gcaagcctta
      721 gttttggcta tgcctatgta tctatctttg tttatcatgc atgaaatata taagctattt
      781 gttttatcaa aaaatgtaga aatacgggta aatattttgg tgaatagtta tcaaaaagaa
      841 ctcaaagaaa gaagacggtg gcggtattta ttgaaaggat gaaaaagtag gatttgacat
      901 actggtattc agaaagtgcc gtcactttta aaacaatgga aaaggtggag ataagatgaa
      961 gaaaaaacaa actaaactat cattagcaga tatttaacac agttaaccgc aactgaagat
     1021 ggtgttgttg aatttgaaca atctgaaatt gcggtagtag atgatcgtta ccttaaaatg
     1081 ccttacttct ttgaccaagc aaaggtcatt tgcctttgtc actacgatgg ggtacgtgat
     1141 tttaggcgtt aaaaagtagt gattatatca actttacgag taaatataaa tttagacatg
     1201 aaactgaatt atccaatgga aaatcatggt ttataatcta gaaaaattat agtaatgatt
     1261 ggattatata tattctttcg tattttcgta caactgtagc ttgtcaaaat tgagaaattt
     1321 aaccgattag gtactttaca tcgttatttg ggtaatttct aaaacatgaa gcttaccaac
     1381 gaataaactg agactatctt attaattgat caatataaga aaatatcttt gaggagatga
     1441 tgtactatgt tttcgtctat ccacgattgg ttaggtggtc ttttcggcgg tggaggtaaa
     1501 ggttttta
//
LOCUS       DACEOE010000307         1496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.320, whole genome shotgun sequence.
ACCESSION   DACEOE010000307 DACEOE010000000
VERSION     DACEOE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.320"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1485
                     /gene="copB"
                     /locus_tag="IW774_002797"
     CDS             <1..1485
                     /gene="copB"
                     /locus_tag="IW774_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1155111.1"
                     /translation="ETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGES
                     RGIKKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDK
                     VAKWLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTS
                     IAAKNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYL
                     GALEANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVE
                     RLGLNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITP
                     VMLTGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPS
                     LARASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYN
                     ILAIPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT          510 a          260 c          340 g          386 t
ORIGIN      
        1 gagacagttt cattgaaaga agtaaacgaa ggtgatcgtt tgatcgtccg gtctggtgat
       61 aaaatgccaa cagatggtgt aatcttgaaa ggtgaaacaa ttgttgacga atcagctgtg
      121 actggcgaat caagaggaat caaaaaacaa gccaatgaca aagtgattgg cggttctatc
      181 aatggggatg gaacgatcga aatcgaagta actggaactg gtgaaaatgg ttatctagcg
      241 aaggttatgg aaatggtgcg aaaagcacaa ggagaaaaat ccaagctaga gtcattatct
      301 gacaaagtgg caaaatggct gttttatgtc gcattaattg tgggtatcct ggcattcatc
      361 gcctggttgt tcttgacaga tcttcctaat gcattagaac gtatggtaac agtctttatc
      421 atcgcctgtc ctcatgctct aggtttagct atcccattag ttgttgctcg ctccacatca
      481 atagcagcta aaaacggatt acttttgaaa aaccgcaatg cactggagca agccaatgat
      541 ctagatgtga tcatgctgga caaaacagga acactaacag aaggaaaatt tactgtaacg
      601 ggtgtcgaag tacttgatga tgcgttcaat aaaaatgaaa tccttcaata tctaggagcc
      661 ttggaagcga atgccaatca tccattggct gttggcatta tgaattattt gaaagaacat
      721 gaaatcaagc cttatcaagc tgaaaatcta aaaaatcttt caggagtcgg gctagaggca
      781 acagtcaaaa aacaacaagt gaaaatagtt aatgaaaaag aagttgagcg tctcggatta
      841 aacgtagaac aagcattatt gaaaccttac caagaacaag gaaataccat cagcttcctg
      901 attttagaaa atcatctagc tgcaatcgtt gctttaggtg acgtagtaaa aacagaagca
      961 aaagaattta tccgaacatt gaaagaaaga aaaatcactc ctgtcatgct tactggtgac
     1021 aataaaaacg ctgcccaagc agtagcagat tatttaggga tcgaagaata ttatggcggt
     1081 ttattaccag atgataaaga agctgttgtc cagaaatacc ttgaccaagg gaaaaaagta
     1141 atcatggtgg gtgatgggat caatgatgcg ccaagtttag cccgtgcttc aatcgggatg
     1201 gcaatcggtg cagggacaga tatcgcaatc gattctgcgg atgtcgtatt gacaaacagc
     1261 gatccaaaag atattttgca ttttcttgat ctagccaagc aaacgcgaaa gaaaatgatc
     1321 cagaacctct ggtggggggc aggttacaat attcttgcaa ttccgctagc agccggaatt
     1381 cttgcgccaa ttggtatcat attaagtcca gcagttggtg ctgtcttgat gtcgttaagt
     1441 acggtagttg tagctctaaa tgctttgaca ttgaaaatca aataataatg aaaaaa
//
LOCUS       DACEOE010000308         1477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.321, whole genome shotgun sequence.
ACCESSION   DACEOE010000308 DACEOE010000000
VERSION     DACEOE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.321"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..184)
                     /locus_tag="IW774_002798"
     CDS             complement(<1..184)
                     /locus_tag="IW774_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1155112.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHN"
     gene            complement(296..1294)
                     /locus_tag="IW774_002799"
     CDS             complement(296..1294)
                     /locus_tag="IW774_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1155113.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
BASE COUNT          459 a          278 c          259 g          481 t
ORIGIN      
        1 cgttatgtac ttcacgcttt gttagcacgc tgttgacact ttcagtacaa tccgccaaag
       61 tcaaatcaga aatatagtct ttttgtaaaa acatgacaag ttgtgcgata tcttctacag
      121 ttacgccacg ttccttcaat agttctcttg ccttttcttc taaagtctca gttttgacga
      181 ccatattatt gctccccctt tattttttct ttctctattt catgttaacg gaacatgaaa
      241 aaaatgcaag aaactgcttt cgggtttaac ttgaaatgca aagagatata aaaaatcaac
      301 taggacgaag atttttatcg tataagcttg tgctgtgacc aggctgagtt cgttcctttg
      361 gattaatata gtgtaaagcg ttgttcactg cagtaggtgc ttcaccgagc ccagtagcaa
      421 tcagtttcac ttttccttca taggtacaga tgtcaccagc tgcataaacg cctggaatag
      481 aagtagacat atctgatttt acagtaatgg catttctggt actatcaagt ccccaagaag
      541 aaagatgttc taaattagaa gagaatccat aattgacgat cagagaatca atcatcaaat
      601 caatcgtctc gtcacttttc acttttttta accggatatc tgtcagttcc ccattattgc
      661 ctgataatcc atcgatgaga tagggagtaa gcaagttgac agatgaagat ttcaagcgtg
      721 atacactatg ttcgtgcgca cgaaattccg ggcgacgatg aatcaagtaa acttcgcttg
      781 caataggctc taacatcaac gtccaatcga tagcagaatc accacctcca gcaatagcga
      841 ctttttttcc agcatacttc atcaaatcat tcacgaaata atccaatccg tggttttcaa
      901 atgattcagc attatctaaa ttcaattttc ttggctggaa tgaaccattt cctaaagcta
      961 aaatgactgc tttggaataa tggatgcctt tatttgtagt aatctcgata acttcgtctt
     1021 cacgagtaag cgagagaact tcttctttta aatggaatgt atggttaaaa gtagttaatt
     1081 gtttttctaa ttgatcgatt aattcacttg cttttattgc tggataccca ggaatatcgt
     1141 aaatatattt ttcgggatac aaagttgcta attgaccacc aagttgaggt agactgtcaa
     1201 tgatttttgt tttagcttgg cgcattcctg cgtaaaaagc ggcaaacatc cctacaggac
     1261 ctgcaccgat gattgtaatg tcatagattt ccataaaaat tcctcccttt tcacactata
     1321 gcagaattta attgagaatg cttctcaaaa atgtttgaat tcgaaatcga aacggtaagt
     1381 tatagaatgt atctagtgat gaaaaataaa agaaaacaaa tctagtattt ttacgagagg
     1441 ttaggtatta tgttattatt attatgaagt atgcaaa
//
LOCUS       DACEOE010000309         1470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.322, whole genome shotgun sequence.
ACCESSION   DACEOE010000309 DACEOE010000000
VERSION     DACEOE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.322"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            181..1368
                     /locus_tag="IW774_002800"
     CDS             181..1368
                     /locus_tag="IW774_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1155114.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          438 a          280 c          296 g          456 t
ORIGIN      
        1 ttcccccctt gggccattga acccgcactc gagagactgg ctcctctctc tgataagtgg
       61 attgcgtatg agttagatgt tgaaagtcct tactttactc cgtatctcgt acaaggtagt
      121 gacgcttttc agttgagtgg atttcaatcc aaaattcttt ttttgaaagg tcagatatac
      181 atgtactttt taggcattga tatcggtaaa cgcactcacg ttgcttctat catgaacgag
      241 gaaggaaagg tattactaaa aggtttttca tttcctaaca ccactgaagg agcagagtca
      301 ttaattgagc gaatggtcga ctattccgga gcacccagtg actttgttat agggatggaa
      361 gccacggggc actattggct gtccatcttt tcataccttc acgaatcaga ttatttgatt
      421 cacgttgtga atccccttca aacagacgga tggcgtaaag gtacagaaat acggaaacga
      481 aaaaacgata ttattgattc tgttttgatt gctgacttaa tgcgctatgg ctcatttgtc
      541 gaaacggtct tatccgatga aaatgttttt tctttgaaac aactttctcg ttaccgaact
      601 tatttagtag gtacagcgag cgacttcaaa agaaaaatta tagccgtatt ggatcaagtc
      661 tttccagaat acgcgacgat ttttaccaag cagggcgttt tcggaaaagc ttctaaagag
      721 ttattgctgg cgtttagtgc cccgatcgac ttagaaaaca tttcctcaga aactttggcc
      781 caatacttag ctgagttaag caggaaccga atcaaggcag aaaaagcgga ccaattgaag
      841 caagcagctt ctaactcctt tggagtaaaa tttgctcaag aagcgttcac cttccaacta
      901 cgttcaatga ttgaacaatt aaaattcatt gaaagccaaa taaaggaaac ggaaagagaa
      961 atcaagcaca taatggacac tttaaattct gttattctca caattcctgg aattggtcct
     1021 ataaacggtg cgactattct tggtgagatc ggagatattc aaaaattttc taacccgaaa
     1081 aaactcgttg cttacgctgg gatagacgct tccgtgacac aatcagggca atatgaggca
     1141 acgcataatg ttatgagtaa acgagggtct ccatatttgc gaaaagcctt gttttcggcg
     1201 gctttagtag cttctcaatg tgatcctgtt tttagtgcgt tttaccagaa aaaagtttcc
     1261 gaaggaaaac atcatctaac tgccttaggt gcggtttctc gtaagctttg ttacgtgatt
     1321 catgctattt tgactaaaaa cgagccttat ataattgttt ctgattagct attgaacatt
     1381 caaaaaaact aatggtaaga agcttatttg ttgtaccctt ttttcggaat tatctgttat
     1441 aaaatatttt ttgatttggc taatttttac
//
LOCUS       DACEOE010000310         1462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.323, whole genome shotgun sequence.
ACCESSION   DACEOE010000310 DACEOE010000000
VERSION     DACEOE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.323"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            159..881
                     /locus_tag="IW774_002801"
     CDS             159..881
                     /locus_tag="IW774_002801"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1155115.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            884..1441
                     /gene="frr"
                     /locus_tag="IW774_002802"
     CDS             884..1441
                     /gene="frr"
                     /locus_tag="IW774_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1155116.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT          533 a          264 c          332 g          333 t
ORIGIN      
        1 ttatgagcca agtgaaaaaa taattctgtt gaataacaga cgggaacgca cggtttgtgc
       61 gctccctttt tttaagcaaa acaagaaaaa ccagtcttag agtgtcgaaa tcatctaagt
      121 atggtaaaat ggcaatagaa aatattggag gaatgaacat ggtcacaccg aaatatcagc
      181 gtgtagtctt aaaattgagt ggagaagctt tagctgggga cgcaggattt ggaatcaaac
      241 caccagtaat caaagaaatc atccaagaaa tcaaagaagt tcacgagctg ggcgttgaaa
      301 tggcaatcgt tgtgggcggc ggaaatatct ggcgcggaca aatcggagca caaatgggca
      361 tggaacgtgc tcaagctgat tacatgggta tgttagctac agttatgaat gccttagctt
      421 tacaggatac gttggaaaac gtgggtgtac cgacacgcgt gcaaacttcg attgaaatgc
      481 gtcaaatcgc agagccatat attcgccgta aagcagaacg tcatttagaa aaaggacgga
      541 tcgttatctt tgcaggcggt accggcaacc cgtacttctc gacagacaca acagcagcat
      601 tacgcgcggc agaaatcggc gcagatgtta tcctgatggc taaaaacaat gtagacggtg
      661 tttattctgc tgatccgaaa gtagatgcaa atgcagtaaa atttgaagaa ctgactcatc
      721 tggaagttat tgccaaagga ttacaagtca tggattcaac tgcaagttca ctaagcatgg
      781 ataatgatat tccactgctt gtcttcaatt taaatgaaca cggaaatatt cgccgtgcaa
      841 tcttaggcga aaatatcgga acaactgtaa gggggaaata aaaatggcag atgcaattat
      901 gacagaagca aaagaaaaaa tgaaaaaagc agcacaaaat cttcaacgtg aacttgggca
      961 aatccgcgca ggccgtgcga acgctagcct tttggaccgt attactgtga actactacgg
     1021 tgcgccaaca ccattgaacc aaatggcatc aatccaaatt ccagaagcac gtgttttaat
     1081 gatcacacct tttgacaaga gcattcttca agatgttgaa aaagctatta tggcaagtga
     1141 tatcggaatc agtccaacca atgatggaaa tgtcattcgc ttagttatcc cgcaactgac
     1201 agaagaacgc agaaaagaat tagctaaaga tgtgaaaaaa gaagcagaaa atgcaaaaat
     1261 cgcgattcgt aacatccgtc gtgatgcgat cgatgaatat aaaaaacaac aaaaaaatgg
     1321 tgatattacc gaagatgatt tgcgcggatt agaaaaagac gtacaaacat tgacagataa
     1381 cagcatcaaa gaagtagata agatcgctgc tgataaagaa aaagaattat tggaagttta
     1441 ataaaatcat aaaaacagag gg
//
LOCUS       DACEOE010000311         1441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.324, whole genome shotgun sequence.
ACCESSION   DACEOE010000311 DACEOE010000000
VERSION     DACEOE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.324"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            124..1293
                     /locus_tag="IW774_002803"
     CDS             124..1293
                     /locus_tag="IW774_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1155117.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          530 a          255 c          233 g          423 t
ORIGIN      
        1 atattctatc aaaaatgtaa gtagtaaatc cattactata agattaaaat aaatgttatc
       61 aagatacaag ctattttagc tttattcttt ctgtaaaaaa gaaaggagac taaataaact
      121 attatgaaaa aaggaaaaca tgctaaaaaa agaaaatatt catttttaat gatcgttgtc
      181 gtgttgttta gccttagtgc gttctctaca ggaactattc ttggatggca attttacaat
      241 aatgaacact ctcagatagt tgcccatgct caatcagctc aaaaaaagtt gatactgact
      301 accaatgaac aacagctcgt tgaaaaaatc gatcaactat taagaaaaaa tgattatatt
      361 ggctcaattt acgtaaagaa aaataatcgc atcatcatgg aaaaaggcta tggctatgcg
      421 aataaactca cgcaacaacc aaatgatcct tctctttatt atcagattgg ttcggttcaa
      481 aaagcattga ctactctttt gattttaaaa caagtcgagc gtgggcacct ttctttagat
      541 actaagctgg ctcaattcta tccacaaatt gctggcagcc agcaaattac tatccgtgac
      601 ttgctgtata tgcgctcagg cttgcaccga actgcttctc caaccatacc gatgactgat
      661 gaagaagtcg ttcaatttgc gattcagcac ttaaaattta cagattatca gacctctcat
      721 tatgattcat tgaactttac catattgact ggaattttga tcaaattaac aaatcaaccg
      781 tatgaaaaat tattgaccga tgatatcatc gaacctttag gattacagca tacaaaattt
      841 tatgaccaag tcaaaaattc atccaatcat gcactatcct atcaaatgtc taaagaggat
      901 gattacttta aaccactgac agaatctgaa acagagatcc ataacgaatt agggactggt
      961 aatatcagta tgtccgtcta tgatttaaat acgctgttta cacaagtttt atctggaaaa
     1021 ctagtttcca aagacttact cttctcatta tggcgtgaaa atgcgaatgg acatccatat
     1081 agtggtgggg tatatagcgg agataattat atcctgtcgc aaggaaatat cgactctttt
     1141 gatacatctg ctatatttag caaagattca caggatgcta tcatcatgga aagcaatata
     1201 aaagctgaca aaactatcaa actgcctact gctgatctaa gaaaccaaat ctatgacctg
     1261 atggaaggaa ctactaagtt ggcaaatagc tgatatgatg aaaagatagc aaatactact
     1321 tctttgaata gcatttgctt aaaaaaagta ctcgactaaa cgtaggaaag agtgtgggac
     1381 aaatctaaaa atcaattttt gctacagact ttaattccaa ataaacagaa cagaaagagg
     1441 t
//
LOCUS       DACEOE010000312         1440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.325, whole genome shotgun sequence.
ACCESSION   DACEOE010000312 DACEOE010000000
VERSION     DACEOE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.325"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            25..>1440
                     /gene="nhaC"
                     /locus_tag="IW774_002804"
     CDS             25..>1440
                     /gene="nhaC"
                     /locus_tag="IW774_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1155118.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
BASE COUNT          411 a          250 c          312 g          467 t
ORIGIN      
        1 acaactaaaa aaggagagat tatcatggga aaaaaaggaa caccaagttt tttagaagca
       61 acacttgtat taatggttat tgttttaggc attgcgattg gcgttgtcgg attaaaggtc
      121 tcacctaata tcacgatctt agcagcgatc ggaatggtca tgttgtatgc agtgataaaa
      181 agatacccga cagattggct gcacgaagga atcatcaatg gcatcaaacc aggaatcatc
      241 ccaatcttta tttttatttt agtcggttca ttgattgctg tatggataca agcagggatc
      301 attccaactc taatggtcat tgggttcaaa ttgatcagtg tccagtggtt tgtcccttct
      361 gtatttgttg tgtgtgcaat cgtaggtagc gctgtaggga gtgcctttac tgttatgtca
      421 acgattggga ttgcattttt cggtattggg acaacactag gcttaaatcc agcgctggtt
      481 gttggagcta ttgtttcagg tgccgttttt ggagataaga tgtctccatt atccgaatca
      541 acaaatctag cagcagctat tgttgatgcc gatttgttca aacacatcaa gcatatgatg
      601 tggtcaactg tcccggcatt tgttgtttct ttctttttat tcatgatttt aggacacaca
      661 agtaaagccg caagtctttc atctattcgt gaagtgactg atgttctaga agcaaacttt
      721 actatttcaa tctggtcact gattccttta tttttaatgt tactgtgtgc atggaaaaag
      781 gtgccggcaa ttttaacgat tttgttgaat atcgctgtag cagtaggaat gatttttatt
      841 caaaacccac aaacttcctt aacgaagttg gctggggtca tcgaatcagg gtttgtttca
      901 aatactggaa accaacaaat cgattcactg cttagccgcg gaggaatcga aagtatgatg
      961 cctactgtgt cattgattat tctaactttg tcattaggtg gattgttgat cgaatttggc
     1021 ttgatctcaa ccgtgatgga acgagtatca agaaagatga cgaacacacc aaatttgatt
     1081 tttacaacat tgatgacaag tatcggagta aatattttta ttggtgaaca attcctatct
     1141 gttattctgc ctggtaatgc atttaaagaa acttatgaaa aagcaggatt tgatccaact
     1201 gtattaggta gaacgctaga agatggtgga acagtaatca actacttagt gccttgggga
     1261 attgccggaa gtttcgtagc aagtacattt ggtattccta cactcactta cttgccattc
     1321 gtatttttca gtttgctttc tcctgtgttt tcgatggtca gtgccttcac aggttgggga
     1381 atctcaaaaa aatttgtgaa accgcaagaa gcagtaagca agacagcgca attagaacaa
//
LOCUS       DACEOE010000313         1432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.326, whole genome shotgun sequence.
ACCESSION   DACEOE010000313 DACEOE010000000
VERSION     DACEOE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.326"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            223..408
                     /locus_tag="IW774_002805"
                     /pseudo
     CDS             223..408
                     /locus_tag="IW774_002805"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            602..772
                     /locus_tag="IW774_002806"
     CDS             602..772
                     /locus_tag="IW774_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1155119.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1196..>1432)
                     /locus_tag="IW774_002807"
     CDS             complement(1196..>1432)
                     /locus_tag="IW774_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1155120.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT          447 a          260 c          237 g          488 t
ORIGIN      
        1 ggttctatca aaaaaattct gagatcaata gacaaaaatt tgatgattac agaggtctct
       61 tacgaaacct tacagaagaa aaaaacattg atcgttgctg cagttctctt ccctgctcca
      121 tgtgtctgta gaaattgtgg ttctgctgtg gtagatggaa ataggaaatt aatggtggtg
      181 aaaaacaaga agaaggaaac cattgtccac tttgaataat ataatcatat gccaatggtt
      241 atacgcctaa aaaagcagcg ctatacctgt aaaaactgtc gaagccattg gaacgctcaa
      301 agttatttta tccgtccaag gcactcgatt tcaaatcatg tcagacataa aatcacttct
      361 ttactgactg aaaaagtgtc gttatttttt atttcgaaaa gttgttaggt atctttgaca
      421 accgttattc gcacattgaa agagttgaga agctatttac caaagtcttc caaaagaatt
      481 ctgtcttgag tgttgagggt tgtgaatttc gttctcacgt ttccgttgag gataaactga
      541 gttttatttc cgcggatggc gagacagaaa aattaatcga gaaaatttac ctacacaaaa
      601 aattcctcga ttaacaaact atttccttag ctgcactaat cctaaagaaa tagaattctt
      661 agttacagac atgaacgcca cctactttca gctcaccaaa catgttctac caaatgcgaa
      721 attggtgatt gatcgattac ttggtttttc cacgcctttc aaggaggcgt aacctttatt
      781 cacgagttgg ttgaagcttt ccgagacaaa agacccgact tgtttttctc cttattgaca
      841 aagagccatt aaaactagag aaaaaattac aactgatcgc tgattatttg tagaaagaaa
      901 atcaaagtat tacccaataa acttgagtca ttaggcaaaa aacactgcga aactcaagaa
      961 tgattttcgc agtgtttttg tatatttttt attaactagt attactacaa aggtttattt
     1021 ttcacttctc aacgcagcac aaaattgtct ttacttattt actggttggg ttcaaccttc
     1081 tctactccga tactaatatt tgtttgtata ccccttctaa aatcggtagt ttccattcaa
     1141 ttgatgggta ctagttgatt ttaaaaacta tgcttattca attttttatt ttagctcata
     1201 tatttcgttt ctttctaaat tttatactga tgaaaacaag tagcacaccc aatattgaca
     1261 atataaactg gctaatgatg gtttctccag tttttggaag atgcttattt ccactgcttg
     1321 ttggattgtt tcctcttata tctgagctat cagcaacttt tctattctct tttgttaaaa
     1381 agatcgccac ttcttgttct ttttcgatct tgaaagttac tggtgtcgca tc
//
LOCUS       DACEOE010000314         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.327, whole genome shotgun sequence.
ACCESSION   DACEOE010000314 DACEOE010000000
VERSION     DACEOE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.327"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(150..1337)
                     /locus_tag="IW774_002808"
     CDS             complement(150..1337)
                     /locus_tag="IW774_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1155121.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACEOE010000315         1411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.328, whole genome shotgun sequence.
ACCESSION   DACEOE010000315 DACEOE010000000
VERSION     DACEOE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.328"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(656..856)
                     /locus_tag="IW774_002809"
     CDS             complement(656..856)
                     /locus_tag="IW774_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1155122.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          471 a          242 c          192 g          506 t
ORIGIN      
        1 ctttttttgt tttcagacga aaattaataa tttctaaata ttctttaaaa aagcttataa
       61 ctattatgcc ttttttaata tataacacag ttatttttaa aaataagaat gctaaatcac
      121 tctacaataa cggcgaaagc acaaaaaata aaaaaattat tacgttatta aaaaaacaga
      181 tgaaagatca ggttttatta gatctcattc tttcatcact tgaagggatt taatgaacta
      241 ctttttcctc tttgtcgttt ttatatattc gtattccttc aaacagtagg atagcctctt
      301 tcaatgcttg atatttgaac tgtccgttac ttaaaaattt agatgtgccg ccctcttcct
      361 agaacatatt cttttttact ttttcattaa ttgacagacc aatcgaacga aactgtaaat
      421 ttcgatagga taatgattgg tcaaactcat tcattttcaa gacatctcga ttcatattca
      481 agataggaat ctttttatca acatcacttt attcgtattt tcctctacca taaaagcttc
      541 acgctccaca aaattgtttt atacactact attcagccca ttcaacgacc aatgaatttt
      601 ttcgtacaaa aaaaccgtct tttctttcga aaagacggtt aaaagtcaat ttttattaag
      661 ctttaacaat gtttgttgct tgagggccac gttgaccatc ttcaacatcg taagacactg
      721 cttggccttc ttctaaagtt ttgaagccgt caccttggat agctgaaaaa tgtgcaaata
      781 catcatttcc atcttcacca gtgataaagc caaaaccttt gtctgcgttg aaccatttta
      841 ctgtaccgtt attcataaat aaattcctcc tgatacgtat caatgtacgt tttttcgttg
      901 caataaatat ttgataggca aaaggagttt tatctatgta gatatatcgc tcaaattacg
      961 tttcaaaata gattactcat ttaatatatc atgctttttc taataacaca aggataatgc
     1021 aatgaaatat aaaattcatt gtcttctatt gtcttcgaat cgaagaaaaa gttgaataac
     1081 cgcgattgat tcactttttg ttttcttgtt tatttgcttt tatctgcaac ttttgacttt
     1141 aaaatttctt aactatgaaa acctttcaat tggtggtata cttataaatg gaatttcgtt
     1201 atcaacgcgt tcgttgcctt tcagcaagta tttaaagaaa ggagaatggc tatgacattc
     1261 tataacaaag atatacaata tcgagtaacc ttggatacag atttgaatct atttgtcgtt
     1321 tttgacaaag aaaataccaa tcatgtagcg acaggtgtta cgatcgaaca agcagtgcaa
     1381 gaattaaaaa aatctgcata aggagtggaa a
//
LOCUS       DACEOE010000316         1398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.329, whole genome shotgun sequence.
ACCESSION   DACEOE010000316 DACEOE010000000
VERSION     DACEOE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.329"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..424)
                     /locus_tag="IW774_002810"
     CDS             complement(<1..424)
                     /locus_tag="IW774_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1155123.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..>1398)
                     /locus_tag="IW774_002811"
     CDS             complement(533..>1398)
                     /locus_tag="IW774_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1155124.1"
                     /translation="VFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQL
                     FENSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDF
                     EHLFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQIL
                     NLNRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILS
                     KITVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYH
                     ELAAFVRVSSI"
BASE COUNT          436 a          245 c          241 g          476 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactt
//
LOCUS       DACEOE010000317         1387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.330, whole genome shotgun sequence.
ACCESSION   DACEOE010000317 DACEOE010000000
VERSION     DACEOE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.330"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            272..826
                     /locus_tag="IW774_002812"
     CDS             272..826
                     /locus_tag="IW774_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1155125.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1080..>1387
                     /locus_tag="IW774_002813"
     CDS             1080..>1387
                     /locus_tag="IW774_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155126.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT          560 a          146 c          240 g          441 t
ORIGIN      
        1 taatttaatt atgtaaatta gctattctaa gaacttctac ggataactgc ccgtctaaaa
       61 tttaattaag agcttagaat cttcatataa gtaaggttaa tttttttgta caaaatagtc
      121 ggaatattct gagttactga aatgatataa aatcataaac taaacccctt gattttcttc
      181 acttttaaca gagtatatat ataagttcat gattatggta aaatatatat ataatcatga
      241 actcagaata tttttaagaa gggaaacaat agtgagctat aatgtacaac cgttaaggac
      301 aaatgaagag atacaagatt ttcagttttg gctaagaaga acaagtaatc cagaaagaga
      361 tagtttttta tttttgtttg gaatcaataa tgggttacgt atgtcagata ttataggatt
      421 aaaagttgga gacatacgag gtaaaagtaa gcctattatt gttgaacata aaacaggtaa
      481 gcgaaaaccg atttttatag ataatttgag agaagagatt ttattatata ctgaaggtaa
      541 agaagaaaat gattggttat ttccaagtcg gcaacaaggt cgacatataa caagggatcg
      601 tgtctatcag atatatgcag atatagcaga aaaattagga agagatgata taggaacaca
      661 tacattaaga aaaacttttg ggtatcacta ctacaagaaa actagagaca ttgctacctt
      721 aatgtttatt tttaatcata gctcgcaagc aattacaaaa aggtacattg gtataactga
      781 agatgaaata ggggcatcgt tacgtggttt taaattggga ggctaattga gatattagga
      841 tatatttttt gtgtataatg aaaacttaga aggtgagatt aatgaagata actgaatttt
      901 cggaagtatt taaaacgttt agtaacattt acaatagaat accagagata gaacaaaatg
      961 ttgtagttga taatgattat tatatagaga atgattttaa taaaccaaga aaatatgaca
     1021 acctatacaa ttttatacac aatagaacaa caataaataa caacagtaac actcgagttt
     1081 tgattaattc agcatatttt catattaaat atttaaagaa atatgagtat gaagaagttg
     1141 tattagatta tgggctaaat agacagtcat ttttaattta tgcaataaat aacggaaatt
     1201 tgccaagcaa taaaaagaac ataattattg aaacatatgc ctatctggag gggggatcta
     1261 aaaaactaat taagtcatgt aatatagagt atcaattatc agtaggagaa attaaatgtg
     1321 ttttcgaaga agatttagaa cttgacttaa aaaaaatttt tgccagcatt catgattata
     1381 taggttt
//
LOCUS       DACEOE010000318         1380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.331, whole genome shotgun sequence.
ACCESSION   DACEOE010000318 DACEOE010000000
VERSION     DACEOE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.331"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            164..1330
                     /locus_tag="IW774_002814"
     CDS             164..1330
                     /locus_tag="IW774_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1155127.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
BASE COUNT          477 a          224 c          298 g          381 t
ORIGIN      
        1 tgtaaaagca ccgtgtagct ccgatgacaa taagcaagca tttttagagc taggtttcct
       61 tgattctaaa tatgcggctt atttcaatga gttggtgctt gaacgcgatt ttaagaaggt
      121 cgaaaacagg cacgactttt cgacgtaagg agaaagaatt tcaatgatag aaacaataaa
      181 tcccgcagaa aacaaggttt ctgcgtacat tcatatccca ttttgcgagc atatctgcta
      241 ttactgtgac tttaataaag tttttttaga agggcagcca gtagatgagt atgtggaaat
      301 gctgctgaat gaaatgagat taatgctgga aagtcatcct acagcgaatt tggaaacact
      361 ttatgtagga ggcggaacac caacttcttt atctgctaaa caacttgatc gactattatc
      421 aggtgctaga gagatcctgc cttttcaaaa tggtaatgag tttactgtag aagcgaatcc
      481 tggtgatttg acccgtgaaa aacttcaagt gatgaaaaac tatggcgtca atcgtttatc
      541 catgggagtg cagacatttg acaatcgctt attgaaaaaa attggacgta aacatactgc
      601 agaagatgtg tatcaaacaa tgcaattttt agaagcagaa aattttacca atgtaagcat
      661 cgatttgatt tatgcattac cgggacaaac actagaagga tatcgggata ctttggaaaa
      721 agctttggct ttggatctgc ctcactattc tttgtactca ttgatcctcg aaaacaaaac
      781 aatgttcatg aactgggtac gacaaggaag actcgaactg cctgatcagg aaacggaaac
      841 cagaatgttt gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc aatatgaaat
      901 aagtaacttt gggctctccg gacatgaatc tcaacataac ctcatgtact ggaacaatga
      961 ccactatttt gggtttggag ctggtgctag cggttatctt ggagatattc gttaccgcaa
     1021 caaaggaccg atccaacact atttacgccc attacgagca aatgaacgcc cggtattgga
     1081 agaagaaaaa ttgacacgaa aaaatcaaat agaagaagaa atgttcttag gactaagaaa
     1141 aaaaacaggg attactaaaa aacatttcta tgatcgctat caaaaaacaa ttgaatcggt
     1201 gtacggtgat gtgctaaggg aattagagca agaagggtta cttgttaatg aagaagaccg
     1261 tgtttatttg acaccaagag ggactttttt ggggaacaat gtgtttgagc gctttttatt
     1321 tgaaaaatag tttgaagaat atttgttttt tatcgaaact tgaaaagaaa aagtttagaa
//
LOCUS       DACEOE010000319         1378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.332, whole genome shotgun sequence.
ACCESSION   DACEOE010000319 DACEOE010000000
VERSION     DACEOE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.332"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(737..1240)
                     /locus_tag="IW774_002815"
     CDS             complement(737..1240)
                     /locus_tag="IW774_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1155128.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
BASE COUNT          548 a          188 c          234 g          408 t
ORIGIN      
        1 gtttttattt gtggaaaatt taatttttag aaaagtgtat cgaaaaatag atagttagtt
       61 tttccacagt ggataacttc gtaagagata aaaataaaaa attagtcaaa gaagggcgta
      121 tagatttctt ttgataggag atatacaaca gaaatgaata aatattaacg tgttttcaaa
      181 cagtataaaa cggtgtggtt tttggaataa aaaaataaca tatctcattt tacgcttttg
      241 ttattttata cttaaaaaat aacgacacag aaaataccgt gtctcagatt tctctactac
      301 ttctaaaaac tactgttttt ttcctttgaa atgccttcaa aaagtaggat caggcagaat
      361 atactggcag aatcaaggag aagggagcct aaataaggag aaaaaccata gaaaatataa
      421 agtaattact tataaaacaa gctttttata gaaaaataat atacctgagt agggtataaa
      481 ttaagacgga attcctttct agattgagat attgactagg aagaatgtta agattttaag
      541 cctttaagct aaaaaaaacc ctttttgtta aataagggga atatagcctg tcagtttcaa
      601 actgcaagtc gtcctgctgc aataatatag tagattagag atgaaaatag atttttttaa
      661 accaatgact gaataaaaag accgtcatag atacacattg gaatgtgtct attacggtct
      721 ttttttacga acgattttat tgaagacgga cagttcgggt caaaaataaa aaagctacga
      781 tcagaaataa gaataaaatg tatcccaaaa taatggctag acggtctaac ttatttgtcg
      841 atagatgttt gtttagaagt tgataggccc ataaacctat cagtcctcct gttgtattag
      901 ttatcaaatc agtgatgtcc gctacgccaa taccgaatag gaattgaatc aattctgcca
      961 agaagcttac aaggaaaata atccctaatt ttttagaaaa agaaagtctc ttgaaattga
     1021 cattcaaaag caccccaaaa ggaaaaaaga caatcaagtt ataagtggtc tcacgtaata
     1081 ccgtcgtatt ttcaaaagaa aaagtagaaa aagggacgag attgacattg ctgtaagaat
     1141 aagtgagaaa ttgtggaaga ttaaatgata gcttgaataa aatcaaccat aatagaaaga
     1201 ataaataaat ccctaataaa aattttgata cataccgcat agacatctcc tccatttaaa
     1261 aagtatacca tcgaaaaccg ctgaaaaatc ataaaaaagt atgaagttta aggaccaacg
     1321 tagttatcgt acgaaaatta gaagcttttt tttgttggga atccggtttc tttaacaa
//
LOCUS       DACEOE010000320         1357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.333, whole genome shotgun sequence.
ACCESSION   DACEOE010000320 DACEOE010000000
VERSION     DACEOE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.333"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(386..1077)
                     /locus_tag="IW774_002816"
                     /pseudo
     CDS             complement(386..1077)
                     /locus_tag="IW774_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          375 a          244 c          189 g          549 t
ORIGIN      
        1 ttctattatt ataataacct gtaacgacta acttcatccc ttcgtctact tcagctagga
       61 agttcaaact atgggaagca atcaaacaat tgaccccatt taaactgaaa cggatcaacg
      121 gtctggcact aacttgcaaa gtacggatct tatctactgt tccatatttt ttctccataa
      181 ccttcacctc aataccatta tacgaacata cattcccttt gtaaagaaga aactctgttt
      241 tatctttttg actttttgtc tattaccttt cttaaaatca tattggcact tcctgcgaag
      301 atgaaggaca tgtactaaaa aaaagaggca gcagtataga ttactgttgc ttctttacta
      361 atttaggttt acttgttaag aaagattatt tacgcaaatg ttcttcaaca atttgctgag
      421 atttatcagc tgtatcttcc acttcatctt ttgctttttc taacttttga atatattgtt
      481 tcaagcttga gttttcagca gttgcttcgt ctaatttctt ttgtgtatct gttaactgat
      541 ttctacattt gttttttctt gtgtcagtgt tgcgatttgt tgattcaaac catttacagt
      601 attttgtaat tctgaaagtt ttgcttgtgc ttcttgtagt cttgctgtcc aatctgcatt
      661 cagatgattg atttcatcat ttttttggtt gatctcatct agtttgacat tgatttcatt
      721 ttgtttgctt tgtagttgtg catccttgct gactaattga ctttctaagt tttgtttttc
      781 tgctgccgat tgattttttt ggttttctaa ttcatttact tttgtttggt attcttggac
      841 atctgccttt gaatcttgca atgtctgttc tttttgtgct agtcgagcgg taagatctgt
      901 aatcgcttgc tctttatctg tcaatgtatg atcaagctgt gtcagattct cattgattgt
      961 atcaatatct gtctctccac cccacaaaat aatttcattt gcgtaccgcg tgcctgtgta
     1021 gccaattaga catagaaatg caacgacaat cccaccagta ataaatttct ttttcatttg
     1081 catagctcct taatccaata cttttacatg ctaaaagtaa gtgatctcaa acacaatgtc
     1141 aagcgttttt aaattacata tgtaatttta atataaatac tatcaaatac tcatttaatc
     1201 ctcctttttt atctatttaa aaaatctttt ttatagagaa aaatgcaggt taatactttc
     1261 ttaaataaat atttatggtg aacaaaaaca aaaaagcaag ttttattaaa cgtaaccctt
     1321 ttcatttcat agattactgt atttattaca aaaaacg
//
LOCUS       DACEOE010000321         1352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.334, whole genome shotgun sequence.
ACCESSION   DACEOE010000321 DACEOE010000000
VERSION     DACEOE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.334"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            11..1350
                     /locus_tag="IW774_002817"
     CDS             join(11..452,452..1350)
                     /locus_tag="IW774_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1155129.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          565 a          175 c          235 g          377 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga
      481 acaagatttg agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt
      541 aaattgaatc tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta
      601 aaaaaattag ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta
      661 aaagaatcaa gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga
      721 tatacaatca atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa
      781 atccgaataa agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg
      841 ttaaaacgaa atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa
      901 ttcaagataa aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa
      961 ataattagtt atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa
     1021 caagcaatta aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg
     1081 cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg
     1141 tctagaaaag gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa
     1201 tcagagtttt tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat
     1261 gagtatatta ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca
     1321 gtagaatatc ggaaacagtc cattaaatag tt
//
LOCUS       DACEOE010000322         1350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.335, whole genome shotgun sequence.
ACCESSION   DACEOE010000322 DACEOE010000000
VERSION     DACEOE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.335"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(88..1218)
                     /locus_tag="IW774_002818"
     CDS             complement(88..1218)
                     /locus_tag="IW774_002818"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1155130.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
BASE COUNT          381 a          267 c          218 g          484 t
ORIGIN      
        1 ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga ctttttggga
       61 cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga cttgctgcaa
      121 taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt ctgttgaagc
      181 aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt gtgcttcttt
      241 tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag taactaatcc
      301 taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat ctcctgctcg
      361 gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact cttctgcaat
      421 gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc agatatgtgt
      481 tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct ctgtacaaat
      541 atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt tagtttgatc
      601 agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt tttcataaaa
      661 agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag atagatcagg
      721 ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc gtaaaatcat
      781 cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca ctacttcttt
      841 cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt tatctactac
      901 ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc cagtttctgg
      961 aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa atccacgccg
     1021 aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca ctaatttttt
     1081 tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt cttgaacttt
     1141 ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat ctactgtaaa
     1201 tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa aaaaatcctt
     1261 ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt tttcagaaaa
     1321 aacattcttt ctctcagcct gacaataaaa
//
LOCUS       DACEOE010000323         1348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.336, whole genome shotgun sequence.
ACCESSION   DACEOE010000323 DACEOE010000000
VERSION     DACEOE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.336"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            15..1310
                     /locus_tag="IW774_002819"
     CDS             15..1310
                     /locus_tag="IW774_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1155131.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          418 a          268 c          260 g          402 t
ORIGIN      
        1 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
       61 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
      121 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
      181 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
      241 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
      301 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
      361 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc gctcgtcaga
      421 aaaatatttc cacttcttcg gtctatcgtg tcatgaagcg attctaccgc ccattgaatc
      481 ctttcaaaca gactttgcca aaggtcttat gctttgatga attcaaatcg gttcgaagtg
      541 ttgccagtgc aatgagtttt atcatgatgg acggacaaag ccatgcatta ttggatattg
      601 tcgaaaatcg acgcttgcct tatttagaac gctatttttc tcggttttca ttagcgactc
      661 gtgaagccgt tcaattgatt gttattgata tgtatgcgcc ttatgtttcg ttggtgaaaa
      721 agcttttccc gaatgctcag ttgatcatcg atcgtttcca catcgtccaa catattggca
      781 gaactttctt gaatcatcga gtgaaagaaa ccacggtacg acttaaagga aattcccact
      841 ctcaacgagg gttagggaaa aaattgaagc gctattggaa actcctacaa aaagaagaag
      901 caaagttaaa ctatgaaaaa cgggtttggc gtgccagttt taaagattat ttgactgaaa
      961 gtgagattgc cgatcgactg ttagctgctt gtcccaactt acgccaaggc tatcagttat
     1021 accaagacat tctttatgcg gtaaagaaaa gagatcagtc actatttgag gattgtttaa
     1081 ctagagacat tagcggtctg ccagaaacct atacaaccac tttacgaacg tttaaaaagt
     1141 ttctgccaca gatccaaaat gcgcttcact acagctattc aaacggacca ttggaatgct
     1201 taaacaacca catcaaagta ttaaaacgca atgcgtacgg ctttcgcaac ttttacaatt
     1261 tcaaactacg aattatgatt cgtcatggca aaacattttt aaccaaataa acagaacaga
     1321 aagaggtttt cttctttctg ttctgtcc
//
LOCUS       DACEOE010000324         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.337, whole genome shotgun sequence.
ACCESSION   DACEOE010000324 DACEOE010000000
VERSION     DACEOE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.337"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1337
                     /locus_tag="IW774_002820"
     rRNA            <1..>1337
                     /locus_tag="IW774_002820"
                     /product="23S ribosomal RNA"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DACEOE010000325         1331 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.338, whole genome shotgun sequence.
ACCESSION   DACEOE010000325 DACEOE010000000
VERSION     DACEOE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.338"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..986)
                     /locus_tag="IW774_002821"
     CDS             complement(<1..986)
                     /locus_tag="IW774_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1155132.1"
                     /translation="MKEERKLPEWLIDYAEKEGLIAELKPKHERQNFLVGDDRLDHAV
                     AFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKF
                     FESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRRKQTFPQSIEIC
                     VDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVE
                     PEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKV
                     CERADGTYNFDRFYSRKANIKDVNAGILISYKAEQYYKGYKKHEHEFVPEVK"
BASE COUNT          342 a          281 c          209 g          499 t
ORIGIN      
        1 tttttcactt caggtacaaa ttcatgttca tgctttttat agcctttgta gtattgttct
       61 gctttatagg aaataaggat tcctgcatta acgtctttta tgttggcttt tctactataa
      121 aaacgatcaa agttgtatgt tccatctgcg cgttcacata ctttcagttc cttcgcgacc
      181 gtggtacatg cttctttcaa atcaattgtt tctactggtt cgcccttcgc caacatcttt
      241 ccaaacgtag attgaacatc atgagcagac accaattgat ttgtcagctg taaattagtt
      301 tcagttttgt gtatggccat aattgcttca ggttcaacac tcatttcttt tgcgactgct
      361 tcataagtag ctttgtattc tttaggtact tgccaatcat ttggaattcc tcgctcacaa
      421 cggattttct tattggtaaa aggatcaacg attcccttca tctgttcttt ttcataaaaa
      481 atatgtcctg cacgatcatt gtccacacat atttcaatcg attgaggaaa tgtttgtttt
      541 cgtcttaatt cagaaatcga ctcttcaacg taatgactga ttacatgatg ttttaacccg
      601 tccatcgaaa ctaaccaggc atcttgtagt ttctcccgat tcaatgcagc gtaacttaac
      661 agatcaatac tactttcaaa gaattttaaa tgcttcggat cgcctatttt taaattaaat
      721 ccgtgatttg gcgtaggatt cttgtcaatg tgtttatacg ttccccgttt accgaaacga
      781 ttaaaatcga cgatcgttcc ttgataactt gcccctactg tctctcctgt ctgtggatct
      841 ttccataaaa aagcaaccgc atgatctaaa cggtcgtctc caactaaaaa attttgccgt
      901 tcatgtttcg gctttagttc agcaatcaac ccttcttttt ctgcataatc aatcagccac
      961 tctggtagtt ttcgttcttc cttcagataa ttccttcctt tttctgtgag tggttgataa
     1021 tcaacggggt gatctttgta cttttctgtc tcaaaatgaa gattttccac tcgttctgtt
     1081 ttgtcttcat tctttagaat aaaatcagtg gctgctttaa attgttgctt agggtcttgg
     1141 atctctttgt ctatgaaaaa aagtttagct aattcaataa tgtcgccact aatattttga
     1201 ctgttccaat aaaaacgttg ttttcttcga tcaaaaacaa atgaatcatg gtcttctaag
     1261 cgatagtctc gtcctttatt gactacagcc attccattgt ttcgagcaaa atccactata
     1321 tcgatctgtt t
//
LOCUS       DACEOE010000326         1324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.339, whole genome shotgun sequence.
ACCESSION   DACEOE010000326 DACEOE010000000
VERSION     DACEOE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.339"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1042)
                     /locus_tag="IW774_002822"
     CDS             complement(<1..1042)
                     /locus_tag="IW774_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1155133.1"
                     /translation="MTEKGRSYLKEQRKFPEWLIDYGEQQGLILEMKPRNERKNYIVQ
                     DDRLDYAIVFPWKDRHTDQILGATYQGTIVDYERFGKRGVDKRIDINPTPNHGFNVLL
                     GSPKNLKFFESPIDLLSYAALNRETIKDTWLVSMDGLKDAVVGHYVREAVTELAPKEE
                     FPESIEICVDNDRAGHVFWDKVHRLGVSNPFTGKAVYFEPNLPNDWQVPREYQTIYEE
                     VGKEMRVAPEAIMAIHKIENNLTDTNQIVSFGEIQGVFAKKLAPKEKAQSIVVKEKCI
                     EAAQQLKSCEKSDGTYDFDRFYRGKGKINEQIQFSLKAKHYFEGYKNHEHEFVSEVKK
                     DWNDQLKHEIQQQ"
BASE COUNT          351 a          294 c          212 g          467 t
ORIGIN      
        1 cttgttgctg gatctcatgc ttcaattgat cattccaatc ttttttcact tcagaaacaa
       61 attcgtgttc atgattttta taaccttcaa aatagtgttt ggccttcaat gaaaattgaa
      121 tttgttcatt aattttccct tttccacgat aaaaacgatc aaaatcgtat gttccatcag
      181 atttttcaca cgattttagc tgctgagctg cttctataca tttttccttt acaacaatag
      241 attgggcttt ctcttttggc gcaagttttt tggcaaatac tccctggatt tcaccgaacg
      301 atacgatttg gttagtatcc gttaggttgt tttcgatttt atgaatagcc atgattgctt
      361 caggcgcaac tctcatttct ttccctactt cttcatagat tgtttgatat tctctcggta
      421 cttgccaatc gttgggaaga ttcggttcaa aatacaccgc ttttcctgta aatggattcg
      481 acacaccaag tcggtgtacc ttatcccaga atacgtgtcc agctcgatca ttatccacac
      541 aaatctcaat cgattctgga aattcttctt tcggcgccaa ttctgtcacc gcttctcgaa
      601 catagtgccc cactactgca tccttcagtc catccataga aacaagccac gtgtctttga
      661 tcgtctcgcg atttaaagca gcgtaactaa gtaaatcaat gggactctca aaaaatttca
      721 gattcttagg agatccaaga agaacattaa acccgtgatt aggcgtagga tttatatcta
      781 ttcgtttgtc aacaccccgt tttccaaaac gttcatagtc tacgatagtc ccttgataag
      841 ttgcccctaa tatttggtct gtatgacggt ctttccaggg aaaaacgatc gcatagtcta
      901 agcgatcatc ttgcacaata taattttttc gttcattgcg tggcttcatt tccaggatca
      961 acccttgttg ttctccgtaa tcaatcaacc attcagggaa ttttcgttgt tcttttaagt
     1021 aacttcgtcc tttttctgtc aatggtcgat aatcaattgg gtggtgctta tattcttgtg
     1081 tttcaaagtg aagattttcc acccgttcca ccttattttc gtcctttata atataatcta
     1141 gcgctgcttt aaaacgttgc tttgggtttt gaatagaatc gtcaataaaa aacaattgag
     1201 tcaattcaat aatatcccca ccaatacctt gactgttcca ataaaatcgc tgttttcttc
     1261 gatcgaaaac aaaagaatca tggtcttcta aacgatagtc tcgtccttta ttgactaccg
     1321 ctaa
//
LOCUS       DACEOE010000327         1320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.340, whole genome shotgun sequence.
ACCESSION   DACEOE010000327 DACEOE010000000
VERSION     DACEOE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.340"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(118..1143)
                     /locus_tag="IW774_002823"
     CDS             complement(118..1143)
                     /locus_tag="IW774_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1155134.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          495 a          266 c          220 g          339 t
ORIGIN      
        1 ttccacattt ttgtgtgaat ttgacttaat gctgagaatc tggcttttga acaccttgaa
       61 ttagaggttg tgacaaaaat cttgtcataa cctcttttgg cacatatatt tgtccttcta
      121 ttctaatttt tgacgaacaa ccgttaaata atgttgcaaa ccaaccgtca ttacccacgc
      181 aacacctaaa ccgatgaaac ttcctatgac gatccctaaa aaacgatatt cgatcaaaga
      241 aatcaagtat gctccacgaa taagtgtaga tagcatcaac gccatcggat taagaaaaat
      301 attagcaact gcataatttc tagcaactag aaattgtgcg attccatata gaaaagtgat
      361 cagaaaaatg gtctgcaact gcgtaaacgg cacaaatgac aagaatgccg acacacataa
      421 cccacccatc gatccaatca gatattggac ttgtcgatgc ttcatcgcat ccaagttttc
      481 tgctagcaaa atcgatgcac atgacaaggt caaccaataa ggattatgca gattcaagcc
      541 gtgactgaca taaacggaaa gaaataaggc agcgctgtag aaaacagaat caatgatcac
      601 taaaggctct tcgtgaaatc tttcttttaa agttgctttt tcaattgtct gctcaggccg
      661 tgaatccagt aattttgtaa tcactgccgc aatcagggca aaaacgacac ccataaaaaa
      721 ataaagtgag acgatcggta attgagcgat tggcagctgc atgctcgttc ccatggcaga
      781 aagcatcgca aaaaacaagc cacctggttt agaaatatgg aataatcgca acacgaatct
      841 gctggaaaaa gcaaccaaag ctacggcaaa tggttcaagc cataaaacat aagtcgataa
      901 gatccccaaa acgaaagcaa ccaacagtcc gcaaccgatc agaaacatac gtttcagcaa
      961 atatttcatc ggtatgtttt gataatagaa aaaagtaaag atccctaatg atgaaaaact
     1021 tgaaatcaaa aaatcatgat ttatgtaacc taaccataat acaataaaga taagtagcat
     1081 cagattcatg atacgaaaag gattatcttg tattttattg aaacgaaaat aagtcttgaa
     1141 caatctatcc ctccttccta gtaattggaa aaaaagctga accaagatga aatgccaatc
     1201 ccgtttgtca gcattaccta gtataacaac tatttcttga tttttcataa acttagtatc
     1261 aaaaagtaaa caacttggag aaattagaac aaaaaacgaa cattccgagt cgttttgaca
//
LOCUS       DACEOE010000328         1305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.341, whole genome shotgun sequence.
ACCESSION   DACEOE010000328 DACEOE010000000
VERSION     DACEOE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.341"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            21..1241
                     /locus_tag="IW774_002824"
                     /pseudo
     CDS             21..1241
                     /locus_tag="IW774_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789773.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          402 a          242 c          283 g          378 t
ORIGIN      
        1 gatcaagagg tgattacacg atggctagaa agaaaagaaa tcctgatgcc gaaaagttag
       61 ctgaatccat tctgaatgcc tatcaacctg aatctgtcga tgacatgcaa gatgctttga
      121 aagatgtgtt tgggcccctt tttgaaaaaa tgcttcaagg agaattgaat aatcatttag
      181 gttatgatgc ccattctaaa gagcctaagg aacacgataa ccgtcgaaat ggctatggaa
      241 ctaaaacgct taaaaccagt tttggtgaag tagctattga tgttcctaga gaccgggaag
      301 cttcctttga accagagtta attcctaaga gaaaacgaga tgtctccgac atcgaaggga
      361 aggttctttc catgtatgca cgaggaatga gtcaacgaga tattgccgca accgtcgaag
      421 ctatttatgg ctttgatatt tcccatgaaa tgatttcaga tatcactgac gctgtccttc
      481 ctgaattgga agaatggcaa gcccgccccc tagcaaagtg ttatgccttt ctattcgttg
      541 attgtatgta tgttacttta cgagaaaatt atgaagccaa agaatatgct gtatacacca
      601 ttcttggcta tgatctcaaa ggaaataagg agattttagg attatggtta aatcaaacag
      661 aatctaaaaa tcgctggatg caagtatttg atgaactgaa agcacgtggt gtcgaagatg
      721 tctttttcat ttcgatggac ggtgtttctg gcctcgaaga aggcgcaaaa gcgatctttc
      781 catcggtaat tgttcagcgc tgtattgttc atcttgttcg aaatgcattg cgctatattc
      841 caagtaagga ctataaggaa gtctgccgag atatgaaaaa gttctacggt gcttcttctc
      901 taaacgctgc acatgctgct tttgacagtt ttcaaaatcg gtggtctcat tattctggtg
      961 ctgtagatgt ctggaaacgg aactttgcac atgttgaaca actatttgat tatggtagtg
     1021 ccattcgaaa aattatgtac actaccaatg cggttgaaag tgtccactcc agcttcagaa
     1081 aagtcacaaa aaaaggagcg ttctctaatg agaatgcact cctaaaacta ctttatttac
     1141 gcacgaaaga attacacgcg aagtggtcag gtggtcgaat tcaaaactgg gctatggtgc
     1201 ttaatcagtt aatgatcaac gagaccttct cctctagaat gaaaagtatg cgatttacct
     1261 tccgtaacaa cggtaggttt attcaactat gtgtccgatc aaaat
//
LOCUS       DACEOE010000329         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.342, whole genome shotgun sequence.
ACCESSION   DACEOE010000329 DACEOE010000000
VERSION     DACEOE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.342"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(45..779)
                     /locus_tag="IW774_002825"
     CDS             complement(45..779)
                     /locus_tag="IW774_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1155135.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..>1294
                     /locus_tag="IW774_002826"
     CDS             1106..>1294
                     /locus_tag="IW774_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1155136.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNL"
BASE COUNT          435 a          240 c          232 g          387 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tcta
//
LOCUS       DACEOE010000330         1273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.343, whole genome shotgun sequence.
ACCESSION   DACEOE010000330 DACEOE010000000
VERSION     DACEOE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.343"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            32..529
                     /locus_tag="IW774_002827"
     CDS             32..529
                     /locus_tag="IW774_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1155137.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(566..1249)
                     /locus_tag="IW774_002828"
     CDS             complement(566..1249)
                     /locus_tag="IW774_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155138.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          443 a          217 c          224 g          389 t
ORIGIN      
        1 tatagtatac tatgtcaaaa ggaggagtta gatgaacaat caaatcacac tagcgtattt
       61 agattatgat gatctagatg ctttacaagt tgtaagtgtt gtcagttttt atgaatcgtt
      121 tatcgatggc gcagatccac ttgatatgca aggatattta cagacacaat tgacaactga
      181 aatattggca gtagaattgg cacagtcaac cagtaaattt attggtatta aggatcatca
      241 aatactcatt ggttatatga aagtcaatga tgaaaaagat gctatagaga ttcaacggct
      301 ctatctgctc aaagattatc aaaacaaagg gttagggcaa cggttactcg atgaagcgaa
      361 tcgttatgct aaaacgaaac aaaaacgtta tttacgtttg actgtttatg aaaaaaatca
      421 tgcaggtatt cgtttttatg aacgtcatgg atttaaaaaa atcgggatca aacattttcc
      481 tttaggaaaa caagatcgta tttgtccgat tttagaaaaa gaaatctaaa aaaacgtcct
      541 cacttttgat gctgaggacg tttttttaga ctggtaatgg aaccataatt cgctttcctt
      601 tttgataaat cgctaattcc gtcacaccat ttgcttttaa cagagcaaaa agatcatcga
      661 aatggaaacg gaaatgttca aggtaatgcc catctgatcc aacactaaac aaggtaccac
      721 cacgtgaaag atataaatcg atgacatatt catataacgc taaattgtca tacaagtaag
      781 cgctcttagc atttaattcg aatgccaatt tatttttgat tacttgatct agaatcggta
      841 aaaactgcgc ttcatattgt ttaaattcag caacggatag accaaatacc cgaaaaccat
      901 agtcaaaatg agcaaagaca tctgcttcta taacaactag tgcttctgaa agttgtgcaa
      961 aatactgagg aatcacaata gcaggatcca tatctttgac ttcgtcatct aaataatcaa
     1021 attgcccatt atgatgcaca ctgagtaatt ttaaatcgta atctttatcg gctaaatagt
     1081 cgatgatccg atccttttca gaagcgatgt aaccgatttc gatgcctttt aaaacacgat
     1141 tgccaaaacg cttatttaag ctatcgattg tttgactgta catttcataa tcggggatat
     1201 catcgacttt agtaacagga ttggctaagt caaaatgttc agttgtcacg acgtaaccat
     1261 cgtagtttgt taa
//
LOCUS       DACEOE010000331         1267 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.344, whole genome shotgun sequence.
ACCESSION   DACEOE010000331 DACEOE010000000
VERSION     DACEOE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.344"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1185
                     /locus_tag="IW774_002829"
     CDS             <1..1185
                     /locus_tag="IW774_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1155139.1"
                     /translation="LEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
BASE COUNT          450 a          253 c          242 g          322 t
ORIGIN      
        1 ctggaaaaag ctgggaaact ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac
       61 accttagttg ctgagatgag cagcgggttg gggatcgatg tcttaggttt agattcactg
      121 ttgaaaaatc taagtggtgg acaacgttca aaggtgattt tagcaaaact acttttagaa
      181 aacccagatg ttctgatctt agatgaacca accaatcact tagatgatca gcatatccaa
      241 tggctgacgc agtttcttca ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa
      301 gaatttcttg atcagatcac cacccatatc gcagacatcg agtttggaaa gattactaag
      361 tatactggcc atctaaaaca agcattgaaa caaaaggaac aaaatcgcga aagctatttg
      421 cgccaatatc atgctcaaca aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac
      481 aaagccggtt cccgatcaac gatggcaaaa agccgacaaa aacaattaga taaaatcgaa
      541 cggctgacgc ctccagcttc agcaagtaaa gctgtctttg attttccatt ctcgcctatt
      601 gtcacgacat tagcagtgga aacaactgat ttagtcatcg gatatgatcg accgctactt
      661 gcacccatca atttgaccat ccgctttggt gaaaatattg ccattcgagg cttcaacggg
      721 attggaaaat ctactttatt aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat
      781 ttccacttcc cagataacac aaaaatcaat tacttttcac aagatctgta ctgggaaaat
      841 ccattagaaa caccactaca atatttaagc agtcaatttc caaaagcgac tatcaaagaa
      901 ttacgtcgcc aactagctaa agcagggtta gttaatcaat tagcaagtga acctctcact
      961 acattaagtg gtggggaaca aactaaggtg aaacttgcac agttgacaat gaaccaaggt
     1021 aatttactga ttctcgatga accgacgaac catatcgatc aagaaacgaa agaaagctta
     1081 caagacggga tacagaaatt tccaggaaca gtaatcatcg tctctcatga acaagaattt
     1141 tatcaagatc ttgtgcaacg agtcattgag attgaaccaa aataaacgtt tgattgaata
     1201 gttgaaaaaa aggagctgtg acaaaagttt tgtcacaact ccttcttctg aataaatggt
     1261 ggccaat
//
LOCUS       DACEOE010000332         1261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.345, whole genome shotgun sequence.
ACCESSION   DACEOE010000332 DACEOE010000000
VERSION     DACEOE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.345"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..522
                     /locus_tag="IW774_002830"
     CDS             <1..522
                     /locus_tag="IW774_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155140.1"
                     /translation="KMISGQSKVLSVNGKYGAVILTKADLGLENAITELPYASETSDG
                     ILTAEMFQKIVNGEGGTYILPIATPEQLGGIKVGELLEVTEEGVLSATKQTDFNFSEE
                     LKGKLESLKILKAGANISIAEDGTISSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNE
                     RIPNFTFEKIGEV"
     gene            526..975
                     /locus_tag="IW774_002831"
     CDS             526..975
                     /locus_tag="IW774_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155141.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            975..>1261
                     /locus_tag="IW774_002832"
     CDS             975..>1261
                     /locus_tag="IW774_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155142.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNG"
BASE COUNT          465 a          186 c          280 g          330 t
ORIGIN      
        1 aaaatgatat caggacaatc aaaagttctt tcggttaatg gtaaatatgg tgcagtcatt
       61 ttaacgaaag ctgatttagg attagagaat gcaattactg aactacctta tgcaagcgag
      121 acaagcgatg gtattcttac tgctgaaatg tttcaaaaga tcgtaaatgg agaaggtgga
      181 acatatattt tgccgattgc tactcctgaa caattaggag gtataaaagt tggggaactc
      241 ttagaagtta ctgaagaagg tgttttatct gcaactaagc aaacagactt caatttctct
      301 gaagaattaa agggaaaatt agaatctcta aaaatattaa aagcaggtgc aaatatatca
      361 attgcagaag atggaacaat tagttctaca ggtggtagtg ggactggtgg tgttaatcag
      421 tcatatgtag atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa
      481 agaattccaa attttacttt tgaaaaaatc ggggaggtat agaaaatgac agatatcgta
      541 aaaataaaac aaagcggtgt acaggtttat ccacaaactc attggaatgc tatagaaggc
      601 aaaccaacaa cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata
      661 ggaaccgttt ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct
      721 agatttaatt ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact
      781 acagctgtag ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg
      841 gatggaattg cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt
      901 ttaatgtcag caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa
      961 gtggggacgg tttaatggct gatattgttc aattaaaaga agacggagtt gctaaatact
     1021 taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca gatggagacc
     1081 aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat aaacgtgttt
     1141 tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac ggtaatacag
     1201 ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg
     1261 g
//
LOCUS       DACEOE010000333         1260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.346, whole genome shotgun sequence.
ACCESSION   DACEOE010000333 DACEOE010000000
VERSION     DACEOE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.346"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..536
                     /locus_tag="IW774_002833"
     CDS             <1..536
                     /locus_tag="IW774_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155143.1"
                     /translation="NGLKSAPRKENLEKHKGKLPDYTGHYQSEITSTGRKKLFGDSSR
                     SVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESKISEDHLIESMK
                     KTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSKKSHGILERSLQ
                     GLIDDQGNSLGENSYFK"
     gene            complement(827..>1260)
                     /locus_tag="IW774_002834"
     CDS             complement(827..>1260)
                     /locus_tag="IW774_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1155144.1"
                     /translation="TIVGTFALVFFLHLWESLHIHFAVDSLLINSLMTGILSGIGCGL
                     VFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGILVVSLLYLDLTHFLYTLLSCS
                     ILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLEQLEH"
BASE COUNT          475 a          216 c          225 g          344 t
ORIGIN      
        1 caaatggatt gaagtcagct ccgcgtaagg aaaatttgga aaagcataaa ggaaaattac
       61 cggattatac cggccattac caatcagaaa tcacatcgac aggacgaaaa aaactttttg
      121 gtgattcctc tcgttctgtc agacaaagtg aaacaaggac agagtccaag catactacac
      181 gaaacgcatc tgttgacacg aaggtgcggg agcgttctta ttttgttcct aaatatgtac
      241 ccgcatcgat tatcccagac cctagagaaa gcaaaatttc tgaggatcat ttaatcgaat
      301 cgatgaaaaa aacggatgac tcttatctgt tgttcgatac agaaccgacg ccgtatcaag
      361 aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg aacaaaaacg ttagaggaaa
      421 caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg tatcttagaa cgctcattac
      481 aaggattgat cgatgatcaa ggaaactctt tgggagaaaa tagctatttt aaataagtga
      541 tatacaaatt tatattcggc tacagttgga aaggaaacgc tgcggcgttt ccttatttta
      601 tactctatcc gaaagtttat atcgtatcct caaatttttt tgagtttaag cgaatttttt
      661 ctcccaataa gtaagaaaat aatggcaaaa taaaaaaaca aactattttc gcagatcgtt
      721 tgcaaaaata gtttattttt ttataaagaa gattttactg aataatatca ttttagttta
      781 cataatgata tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag tgctctaact
      841 gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca
      901 aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc
      961 taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac
     1021 caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt
     1081 gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa
     1141 tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta
     1201 tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc
//
LOCUS       DACEOE010000334         1248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.347, whole genome shotgun sequence.
ACCESSION   DACEOE010000334 DACEOE010000000
VERSION     DACEOE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.347"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..237
                     /locus_tag="IW774_002835"
     CDS             <1..237
                     /locus_tag="IW774_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1155145.1"
                     /translation="GAFAKGSFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGK
                     RLNMDFNKQLDSFSLENEIAVLDYNIKLKNCFFS"
     gene            271..1209
                     /locus_tag="IW774_002836"
     CDS             271..1209
                     /locus_tag="IW774_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1155146.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
BASE COUNT          447 a          197 c          244 g          360 t
ORIGIN      
        1 ggtgcttttg caaaaggcag cttttttcaa atcattcctt ttgtttcgga atttggagaa
       61 tatgcgacta aaatcgagca tccattattg gacatctata catctaaatt agagcaaaat
      121 tatttcggta aacgattgaa catggacttc aacaaacagc ttgacagctt ttcattagaa
      181 aatgaaatcg cagtactcga ttataatatc aaattaaaga actgcttctt cagctaatag
      241 tttcgagaaa gtccaaaaag gaggtataca ttgagatact actttgtgga tgaagaaaag
      301 aatgtaatag aaacagatga aaaaaaatat aattggctcg tacttgatag tgcaaagccc
      361 gaagagattg agcaagcaat cgaagaattc gagcttcctg atgatatttt cgtaggaacc
      421 gaatatccgg aagaagtttc tcggatggag cgcctttatg agacgaaact agagcatcca
      481 ttttctttgg tagtgattaa tctagatcag tcagataatc gtatcgaaaa aaaattaacg
      541 ccaatatctt ttgtactttc cgataatctc ttgattactt gtatcgatag aaaaaccgaa
      601 tttgtggatc ggctgctgac aaagcatgga aatcatttaa actcgtttga aaagatcatc
      661 acgtatacat tattagatat ttacacccat tacgtgaaag aactaagaga gatgaaaaag
      721 cgaattgacg ctttggataa agcagctcgt aaaacaacgg aaaatgaaga actgtataaa
      781 caggcagatc ttgaaagaga catcgtatat atcgatcata ccttaagaga tcaacgggaa
      841 acaatggatc aattatggaa ttctgaaact ttcatcaaac gtttagacga tgaacagcta
      901 ctctatgatg tccatttgag acagcgacaa acagaaaaaa tgatcgagat ctatcgagat
      961 ttattagaga gtattggtga tctattcaac gggatgatgg acaataatct aaatcatatc
     1021 atgaaatatc tagattcagc tgcattaatc atttccgttc cggcgttgtt cgcagggatt
     1081 tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt tatactcgta
     1141 gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg gaaagattat
     1201 acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgt
//
LOCUS       DACEOE010000335         1247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.348, whole genome shotgun sequence.
ACCESSION   DACEOE010000335 DACEOE010000000
VERSION     DACEOE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.348"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..297)
                     /locus_tag="IW774_002837"
     CDS             complement(<1..297)
                     /locus_tag="IW774_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1155147.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEE"
     gene            531..1232
                     /gene="nagB"
                     /locus_tag="IW774_002838"
     CDS             531..1232
                     /gene="nagB"
                     /locus_tag="IW774_002838"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1155148.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
BASE COUNT          432 a          211 c          242 g          362 t
ORIGIN      
        1 ttcttccttt tcgtattctt ttgcgtacgc agtttttaga aattcttcga ttgttaatgt
       61 ttcatcgact tccacatatt gatcgaggta acctttgtga atattttttt gccaatggat
      121 ctgtccgtca tctggtaaga tctcaccagt gatgatttta atcaatgtac ttttaccggc
      181 tccgttttgt ccaatcagcc caagatgttc gccagcattt aattgcagat cgacaccttc
      241 atagaggatt ttgtcgccaa attgctgggt aatgttgttt aatgtcagta tactcataat
      301 aactcctttt tcataaaaaa actgcagaca taaagcgtcc acagtgaatc acttgcttgg
      361 ttttcatgca acatggattg tttgattgaa ccatcttatg aaacctaaca cggattccta
      421 tttattgtca agaaaatgaa aaaaatgcaa tttggtctat actaattatt gtaaaaagaa
      481 aaaaagaatg ctataattgg gctataccaa aatataaagg agcaatcact atggaaatca
      541 ttcgtgtaaa aaacgctgaa gaaggcggaa aaaaagcatt tgaaatcatc aaagaaggta
      601 tgaacaaagg tgcaaaagta ttaggattgg caacaggaag cacaccagtt actttatata
      661 aagaaatgac ttcaagcgat ttggatttca gtgaaatgac ttctgtaaac ttagatgagt
      721 acgttggact aggcggttca gatgaacaaa gctaccgtca tttcatggat gtccaattat
      781 ttgataaaaa accattcaaa gaaacgtttg taccagatgg aaaagcagaa gatctggaag
      841 cagcatgcaa aaagtatgat caaatcatcg atgcacatcc aatcgatatc caaatcttag
      901 gaatcgggca aaatggacat atcggtttca atgaacctgg tgcttcattt gacggaacga
      961 cttcagtagt tgacttaaca gaatcaacaa tcaatgcaaa caaacgattc ttcgataaag
     1021 cagaagatgt accaacaaaa gctgtttcaa tgggaatcgg ttcaattatg aaaagtaaag
     1081 aaatcgtctt gatggcattt ggcgaaagca aagctgatgc aatcaaaggg atgattgaag
     1141 gacctgtaac gaatcatttg ccagcaagtg tattgcaaaa tcacgacaat gttgttgtga
     1201 tcatcgatga agaagcagca agtaagttgt aattttcaat agtaggc
//
LOCUS       DACEOE010000336         1223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.349, whole genome shotgun sequence.
ACCESSION   DACEOE010000336 DACEOE010000000
VERSION     DACEOE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.349"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..949)
                     /locus_tag="IW774_002839"
     CDS             complement(<1..949)
                     /locus_tag="IW774_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155149.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPE"
BASE COUNT          287 a          219 c          234 g          483 t
ORIGIN      
        1 attcaggcgc tgttgattct tctgttgaag attcaggtgt tgttgattct tctgttgctg
       61 aagattgtgt agctgatgat gattcttcag tcattgaaga ttgtgtagct gatgattctt
      121 ctgttgctga gctttgtgca gtagatgaag cttgagtagc ttgtgcttca gcctctgctt
      181 gttcgcgtgc ttctttttca gctttttctt gtgcagcttg ttgacgagct tgttctgcta
      241 gacgttgttg ttcacgaata cgagcttgct ctgcttcagc ttctgctttt tgacggttca
      301 agtcagcttt cttgtcttca gcagttgctt gttcagcagc taatgaagtt ttcagtacgt
      361 tcaaatcagc ttgtttagat aaaagatcgc ctttttgaga ttccaaagca gcttggtttt
      421 cagctaattc ttttagttta gcatcgtttt cagctttttt gtcttctaca gcttttttat
      481 cttgtttttg ttgttccatc aagtcgttgt ttgcttttac catcgttgtc attgcttgaa
      541 cgcgtccaat agcatctgct aatgaatcag cattcaatac agcatcgatg tagtttgagc
      601 ttgtgttgct tacttgtgct tcgcgtgctt gtttttggat tgtatcttca cgtttttcaa
      661 tacgttcttg taaatctgca atgtctttta ctaattgtgc agattcttga cgcaatgtat
      721 cttgttttgc taataggtct tgtgcttgtg tgttgatttc agaaacttgg ctttctaagg
      781 catcgatttg tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc ttttgatctt
      841 gctgttgtat ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat gctacggcag
      901 tcaaggtcat tgaacatacc attactgctg atattaaact ctttttcact cttcattcct
      961 ccgactggct taatttaata aatatctaaa tatatttaag tctttcttaa cgatataaaa
     1021 attgtaccat agcagtgtgt caacgatata atagttatgt tacaaaaatg tttcaaaaca
     1081 acttttaaaa atgacaaaac tgttcgtttc agaaaatgat catgaaaaag ccgcgttaag
     1141 tctagcattt cttaagataa caacgattct ttttcgcttt cgacctaaaa ataaaaaaag
     1201 aaattattca tcactgaata att
//
LOCUS       DACEOE010000337         1218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.350, whole genome shotgun sequence.
ACCESSION   DACEOE010000337 DACEOE010000000
VERSION     DACEOE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.350"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..569
                     /locus_tag="IW774_002840"
     CDS             <1..569
                     /locus_tag="IW774_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1155150.1"
                     /translation="FVTQKKESLKLAGVHGFSDSLSTRGHKIRYTVLHSKIIKADEFL
                     ANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGSQYTSLYKLLEDD
                     YDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQPFHYSQSTVRCD
                     RISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(667..>1218)
                     /locus_tag="IW774_002841"
     CDS             complement(667..>1218)
                     /locus_tag="IW774_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1155151.1"
                     /translation="LSYPLYSYQKKIGTTASIFDDIEKYNEGCNSLELKGKFTKYWKS
                     QKIDYLDIFPNILIEQLEWLKKKKIKMAIASSSPMDTINEVVTTCKIESYFDCLISGR
                     DLPESKPNPTIFLKASEQLRIPVEECLVIEDSYNGIKAGKRANMKVLAIKDKRFSQDV
                     SLADDVIYDIKYLRQEVQVKFGI"
BASE COUNT          421 a          194 c          167 g          436 t
ORIGIN      
        1 cctttgtaac tcaaaaaaaa gaaagtttaa aattagctgg agttcatgga tttagcgatt
       61 ctctttctac ccgaggacat aaaattagat atactgtttt acattctaaa attataaagg
      121 ctgatgagtt tttagctaat aaattacaaa tcaatgtaac tgatagagtt tacaatatta
      181 aacgtttaat gtgggaagac ggtgccccga tgggaattga taattttttc gcatcagaag
      241 aaaaatttcc aactttattt gaaaaaggta gccaatatac ttcattatat aaacttctag
      301 aagatgacta tgatgtcaaa gctacgagct ctattatgga aataaacggt ttatctgcaa
      361 cacctgagct ttccgactta ttgcaatgta tagtaggaga tccgctattt attttagaaa
      421 aggttggcaa agataaaaat aatcaaccat ttcattattc tcaatccaca gttcgttgtg
      481 atagaattag ttatgttatt gaagtcacag aaaatatcac tttagatacg gtgcataaaa
      541 atgattcaga tcctaaagta atagattgat attaaaagaa atcataccca aagcaatcaa
      601 ttttgattag atttgggcat gatttttatt tttactaata ttctctaaat acatcaaaaa
      661 acttatttat attccaaatt tcacctgtac ttcttgtcgc agatatttaa tatcgtaaat
      721 aacatcatct gctagactta catcttgaga aaaacgttta tctttaattg caagaacctt
      781 catatttgct cttttaccag cttttatacc attataagaa tcttctatca ccagacattc
      841 ttcaactgga attctgagct gttctgaagc tttaaggaat atagtaggat taggtttact
      901 ttctggcaaa tctcttccgc taattaaaca atcaaaatat gactctattt tacaagtggt
      961 aacaacttca tttatagtat ccattggact ggaagaagca attgccattt ttattttttt
     1021 cttttttaac cattcaagct gttcaattag tatattagga aaaatatcca agtaatcaat
     1081 cttttgcgat ttccaatatt tagtaaattt tcccttcaat tctaaactgt tacatccctc
     1141 attgtacttt tctatatcat caaaaatact tgctgtagtt ccaatctttt tttgataaga
     1201 atacaaagga tagcttaa
//
LOCUS       DACEOE010000338         1213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.351, whole genome shotgun sequence.
ACCESSION   DACEOE010000338 DACEOE010000000
VERSION     DACEOE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.351"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1161
                     /locus_tag="IW774_002842"
     CDS             <1..1161
                     /locus_tag="IW774_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1155152.1"
                     /translation="QEAEEQKVLASREEDFEKAAYYRDQINKLQKMKERQLTEEETPV
                     ITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVDRVAKAIRRNR
                     VGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSEYMEKHSVSKL
                     IGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQILDDGRLTDAQG
                     RTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTPEFLNRFDGII
                     EFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYDPSMGARPLRR
                     TIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAEETAE"
BASE COUNT          423 a          226 c          253 g          311 t
ORIGIN      
        1 caagaagcag aagaacaaaa agttttagct tctcgagaag aagatttcga aaaagcagct
       61 tattatcgcg atcagatcaa taaacttcaa aaaatgaaag aacgtcagct cactgaagag
      121 gaaacacctg tgattaccga aaaagatatg gaaaaaatcg ttgaacaacg gacaggtatt
      181 ccagtgggtg aattaaaaga aaaagaacag acacaactga aaaacttagc agatgacttg
      241 aaagctcatg tcatcggaca agataacgct gtagatcgag tagcgaaagc gattcgccga
      301 aaccgtgttg gattaaacaa acagaatcgt ccaatcggct ccttcttatt tgttggacca
      361 actggtgtag gtaaaacaga gcttgcgaaa caacttgcct acgaactatt cggttcacaa
      421 gattcaatga ttcgtttcga tatgtctgaa tatatggaaa aacatagtgt ctctaaattg
      481 attggttctc ctccaggtta tgtcggatac gaagaagctg gtcaattaac agaaaaagta
      541 cggagaaatc cttacagtct tgtcttgtta gatgaagtag aaaaagctca tccagatgtt
      601 ttgcatatgt tcttgcaaat cttagatgat ggtcgattga cagatgcaca aggaagaacg
      661 gtcagcttta aagatacgat catcattatg acaagtaatg ccggaactgg taaagtagaa
      721 gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc gttctgtatt gaaccaatta
      781 aacaactact ttacaccaga atttctgaac cgttttgacg ggatcatcga attcagtgca
      841 ttatcaaaag aaaacttgat gacaatcgtc acactaatgc tagacgatgt caaccaaatg
      901 ctggcagcac agcaacttca catcgaagtt cctacgaatg tcaaagaaaa attagttgac
      961 ttaggctatg atccatctat gggtgctcgt cctcttcgac gcacgatcca agaacaaatc
     1021 gaagatggta tcgctgaatt ttatcttgat cacccttcta tccatgagtt gaaagcaaaa
     1081 ctggataaag atggaaaaat catcgttact tctaaaccag aacgtttggc taaagaatca
     1141 gccgaagaaa cggctgaata aatccagttt tatagcgtga gacggtattt ctttgagaaa
     1201 tgccgtctca ttc
//
LOCUS       DACEOE010000339         1205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.352, whole genome shotgun sequence.
ACCESSION   DACEOE010000339 DACEOE010000000
VERSION     DACEOE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.352"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..476
                     /locus_tag="IW774_002843"
     CDS             168..476
                     /locus_tag="IW774_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1155153.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQLPLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY
                     "
     gene            503..802
                     /locus_tag="IW774_002844"
     CDS             503..802
                     /locus_tag="IW774_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ1155154.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEIGTLPKDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            976..>1205
                     /locus_tag="IW774_002845"
     CDS             976..>1205
                     /locus_tag="IW774_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ1155155.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIA"
BASE COUNT          451 a          167 c          247 g          340 t
ORIGIN      
        1 atttagaaaa acttactaaa caaagagata tgaaaaactt taataattgt gtagttttga
       61 taattataac tgaattaact ttatttacaa atatatatta tatccatgaa gcccaatctt
      121 ctttaataga agattgggct atttttatag caaaggaggg aaaagaaatg tttcaaaata
      181 aaggacaacg ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt
      241 tatgttggaa tctaatcgat caaaacgttc aaaagcaact tcctttagac tatcttcaaa
      301 tttttgaatt ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccgg
      361 aagaaagaaa agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa
      421 tatgggccat tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactagtatc
      481 taaaagaaag gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc
      541 acagctggtc actgcttctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga
      601 aattgaaata ggtacacttc ctaaagatgt tcatctacca cgtttagtga tgacacatct
      661 ttatatttat tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat
      721 taccagccag cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca
      781 gattggagga acggatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag
      841 atatccttac cgcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt
      901 tcaactagta ttgacactta taacggcaat ggtaattgaa gtgcttgaac aaaaaatagc
      961 ataggagaaa aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga
     1021 gatcctcatc atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa
     1081 gcggaagata aattagcagt agtcattact attcgtccaa cagatacaga accatttacg
     1141 aatgatttga gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt
     1201 attgc
//
LOCUS       DACEOE010000340         1196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.353, whole genome shotgun sequence.
ACCESSION   DACEOE010000340 DACEOE010000000
VERSION     DACEOE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.353"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1029)
                     /locus_tag="IW774_002846"
     CDS             complement(<1..1029)
                     /locus_tag="IW774_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1155156.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKIN"
BASE COUNT          321 a          266 c          209 g          400 t
ORIGIN      
        1 atttattttg tattccgtta caaactcgcg tacttggtta ggttccagag tgacatctcc
       61 aaaactttca agtcgctccg ctcctggcag ttcttgagtc tcaaatgtga taccgccatg
      121 atttactaat ttattcccgc gcatttccgg tccatcttct ccaaaatttg ctgtaaaaat
      181 cacgattcct gaagcatctg tttttacttg gatcgatact ttttcatctg ggcttgtcaa
      241 ttccgcagca tttagtaaac cagattgttt tttcaacaaa aatggatgat cgatgccgcc
      301 cactatcatt gattggcgaa aatcactggc aaagacctca cctaattttt taggacgttg
      361 gaagtcaaat gctgtgccac ttactgaaac tacttctcct gtaggcgttg tatctttatt
      421 gagcgtagca aataatcggc tattcaacca aagtatatgt tcgtcgattg actgtgctgg
      481 atcaccggtt aaattgaaat agacatgatt tgtaggattg aatactgttt tgcggtcact
      541 gatcccttgg accgtcactc gccatgtatc atcgttcgtc agcgtatacc gtacttctac
      601 atctaaagtt ccaggaaatc cgttttccat atctgggcta gtctgataga aaatgaccga
      661 tgcttcgttt tcaccattga tgactgtata atgccactgc ttggcttcaa agctaggtgc
      721 accaccatgt aaacaatgac cagtgtccgg gtctgttttt acttcgaacg tttcatcatc
      781 taatttgaat gtgccattcg taatccgtcc agccatacgt ccgattgaag caccgatata
      841 agggtctttt tctagatatt cttttgcttc atcaaaacct aagatcaact ctcgtttttc
      901 attatcgatt attgtttgaa aatgaacaac ccgtgcccca agatcactga cagacaaaga
      961 tgtcccattt tgattcgtga tcgtaatcaa gtgatagttt tcgccaaaat tttgttcatt
     1021 gatttccatg ttccttgcac cctcttttct ttcatttgca ctgattccag catacatgga
     1081 aaccgttttc ttaacagctt attccgtgag taacgagaaa aaatttacta tattctaata
     1141 aaagaccgtg aaaaaaaggt tgtgacaaaa atattgtcac aacctctttt tccgaa
//
LOCUS       DACEOE010000341         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.354, whole genome shotgun sequence.
ACCESSION   DACEOE010000341 DACEOE010000000
VERSION     DACEOE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.354"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1076
                     /locus_tag="IW774_002847"
     CDS             <1..1076
                     /locus_tag="IW774_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1155157.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACEOE010000342         1189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.355, whole genome shotgun sequence.
ACCESSION   DACEOE010000342 DACEOE010000000
VERSION     DACEOE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.355"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..854
                     /locus_tag="IW774_002848"
     CDS             <1..854
                     /locus_tag="IW774_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ1155158.1"
                     /translation="ISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLV
                     LFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRK
                     VEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDF
                     YGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFS
                     LLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLY
                     LKKGELI"
     gene            851..>1189
                     /locus_tag="IW774_002849"
     CDS             851..>1189
                     /locus_tag="IW774_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155159.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNP"
BASE COUNT          398 a          143 c          194 g          454 t
ORIGIN      
        1 taattagtgt attaatttca aaaataacaa aaacgattcg tgttttttat atgatgaatt
       61 ttatcgtaca aatattttat attacacttt cttcgtctag aggtgcccag gtagtaattt
      121 gttctttttt agtattattt cttttgttgt ttgccaattc aaaatttaga aagagcatta
      181 gagaagtact acctgtaaaa atcaaatgga tggtaagcat attattagtt ctattggtat
      241 atttttcttt tggtactaat tatgctaagg attttttagc aacaattcct gtatcgattg
      301 atagaaaagt agaagttata gagtcaaatg aatcagataa aactccactt ccagaaaaag
      361 aagtaatatt acagcattct agtgataact ccgaaatttc agctggtcgt tttacgattt
      421 ggaaagctgc gcttaaagtt ataagaccaa ctttgttatt tggttatgga gagaccgatt
      481 tctatgggaa tagcgatgca aaatttttga aaaaagtaga tcttgatact acagatgtga
      541 atgaactaaa aagagcacat ggaaacatgc ataatggatt tcttcaagta cttgtatcaa
      601 gtggtataat tgcttttata tgtatctatg ctttctatgt gttaaatatc tttagtttac
      661 tcaaaacatt ttttagaagt aaagtgaatt atgcgtttct tggtattgtt ttaattttta
      721 tactatctat ttttatagat gaaatggtgg aggctcatgt acttttcaat aagcgcgatg
      781 tcatttcaat tgttttctgg tattatttgg gaagcatttc ttacttgtat ttaaaaaaag
      841 gagaattaat atgaagacag ctttcattgc ttggactcct ttacatttga ttaatatatt
      901 aaatgtttat caaaattttt accgagaatc aaaaggagat ttatttattt attctgaatt
      961 tgaaaacgct agaagatata gtgagcgtat aaaagaatta gggctattta atgatgttgt
     1021 ttttgtgaat caccggaaaa tcggttcttc ctttaaacgt aaaattaact tgttaactaa
     1081 ccataatcaa tttttctcgg atgatttatc cgtttatgat gaaattttta tacaggggga
     1141 aaattatttc acaaaaatac ttttctccaa tgtttacaaa aaaaatcca
//
LOCUS       DACEOE010000343         1187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.356, whole genome shotgun sequence.
ACCESSION   DACEOE010000343 DACEOE010000000
VERSION     DACEOE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.356"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..391
                     /locus_tag="IW774_002850"
     CDS             <1..391
                     /locus_tag="IW774_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1155160.1"
                     /translation="PSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAATEG
                     GVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNEDE
                     IRSFVPREIRCVARNLIYLQNNNILNS"
     gene            405..761
                     /locus_tag="IW774_002851"
     CDS             405..761
                     /locus_tag="IW774_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1155161.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          423 a          176 c          224 g          364 t
ORIGIN      
        1 gcctagctgt acttcctgta gaaaagcaag agcatggttg caagcgaatc aacttgaatt
       61 tgaagagaag aatatttttg ccgaaccgtt aacagaaagt gagatcaaaa agatcttggc
      121 agcgactgaa ggaggagttc aagatattat ttccactcgg tcgaaaattt acgaaaagct
      181 gggcatagat tttaacgaat taactttaaa gcaaatgatc gtcttattca aagaatatcc
      241 ttcattattg agacgaccta ttttgattga tgaaaaaaga atactaatcg gatttaatga
      301 agatgagatc cggtcatttg tccctcgtga aattcgatgt gttgccagaa atctgatcta
      361 tttacaaaac aataacatct taaattcata gggagggagc cttcgtggaa gtgaaagtct
      421 atggatcagc gcactgtgta acaagtctga aggcgaaaca atggttagaa caccatcaat
      481 tgccttatca atttgttgac ttagaagaac gcgatctaag tacagaagaa gcagaagaat
      541 tatgcagttt tgaagaagtg caagcagaac agctatttgc gacttggtct gaggcttttc
      601 acaacattgc gattgacctt tcctgtgtag gaaaaaaaca actgattttt ttatgcacac
      661 aacataccaa tctcctcaga cgtcctttga ttattatcga tgaagttcta tttattggat
      721 acaatgagca gttgatggaa cagcttatct taaataaata gactttaact gttttaatca
      781 agtcactgag catttcatac agttgtaaca gtcgaacact tagcatcaag cgtgttatgt
      841 gttctttttt tgactttatt tttctactat agagacatgc tcaatttatg tggtcaaaat
      901 tcaaatctag gtggggatat agagttaaaa atatattgac atgtgtaaat ttacatgata
      961 tattcatttc tgttgtttta agtagcaaag caataaaata gtattttaaa aaacatataa
     1021 aatagttgtt gacatgttag aaatgacatg ataagataac tgagttgtta aaaaagaacc
     1081 acttgtgaag cttgattcta tcgaaaaaaa cttcaaaaaa gtccttgaca aataacaaat
     1141 gataagttat gataaatgag ttgctgtaag atttcgaaaa acgaaat
//
LOCUS       DACEOE010000344         1186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.357, whole genome shotgun sequence.
ACCESSION   DACEOE010000344 DACEOE010000000
VERSION     DACEOE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.357"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(34..1017)
                     /locus_tag="IW774_002852"
     CDS             complement(34..1017)
                     /locus_tag="IW774_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1155162.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          329 a          261 c          226 g          370 t
ORIGIN      
        1 acgacctcta attcacggtg ttcaagcaag aaatcattgt tcactgaaaa gcgttggatg
       61 ttctttttca aaggtctcta agatctctcg tacttcatca gtagatttcg tattcatcaa
      121 ctgaactcgc aagtcatttg ctcctggaaa tcctttgata tagattttaa agaaacgatg
      181 tagtcctgtg atggaacgcg ggatgatttc tgaataatga tcttgcagat ctagttgcag
      241 acgaagtagt ccgatcagtt ctttttctgt atgttccctc gggtcttttt caaatgcata
      301 aggattcttg aaaatccctc ggccaatcat gatgccatca acgccatatt tctcggccaa
      361 ttcttctcct gtctggcgat cggggatatc tccattgatc gtgattagtg tttgtggagc
      421 gatttggtca cgtaaagcaa tgattttagg aatgacttcc caatgtgcat ctactttact
      481 catctcttta cgcgtacgca aatggacaga aagattggct atatcttgat gaagtaggtg
      541 actgatccat tcttccatct cagacatttc cgtatagccg atacgcgtct tgacgctgac
      601 aggcaaaccg ccagctttag ctgcttcaat caattgtgcc gctacttctg gacgaaggat
      661 caagccactg ccttttccac gttctgctac attagggact gggcagccca tgttgatatc
      721 gattccttgg aagcccattt ctgccatagc aatgctcatt tctctaaaaa attctggctt
      781 gtctccccaa atatgggcaa ccatcggctg ttcatcttct gtaaatgtta atcttcctcg
      841 tacactgtct tttccatctg gatgacagaa actgtctgag tttgtaaact cagtaaagaa
      901 gacatccggt gctgctgcat gctttacgac atggcgaaat acaacatctg ttacatcttc
      961 cattggtgct aaaacaaaaa aaggctttgg cagctctttc caaaaattag tagtcattta
     1021 tttgttactc tccattctat caagcagtga agtcgcaatc tttctcaaaa acattgtcat
     1081 tatactagga aacaaaaaaa gaagcaaagt gaacagctta aaagatgttt ctgattgagc
     1141 aaaaaaaaga ctgtgacaaa aatcttatca cagtccattt tccgga
//
LOCUS       DACEOE010000345         1185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.358, whole genome shotgun sequence.
ACCESSION   DACEOE010000345 DACEOE010000000
VERSION     DACEOE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.358"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW774_002853"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW774_002853"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1155163.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT          333 a          252 c          206 g          394 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaa
//
LOCUS       DACEOE010000346         1141 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.360, whole genome shotgun sequence.
ACCESSION   DACEOE010000346 DACEOE010000000
VERSION     DACEOE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.360"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..>1141
                     /locus_tag="IW774_002854"
     CDS             128..>1141
                     /locus_tag="IW774_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1155164.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSE"
BASE COUNT          403 a          202 c          236 g          300 t
ORIGIN      
        1 gcgtatactt aaaaaagtag aaatatcaga accttaaaag gatataattt gaagaatata
       61 gaacaaatgt tcagtatttt ttgatataat aaaggagcac cgtatcatgt gttgaaggag
      121 gaaaagcatg tctttacaat ttattcttgg aaacgggaaa aaagatcacc gtcaagtctt
      181 gatcgacgaa gcatccgctt ggctcgacca ggatgcaagt aaccaagtat ttttccttgt
      241 tcccaactac agcaaattcg aacaggaaca agaaatttta gcggagatgc gaagaagaag
      301 cggaaaaaaa actttcagca caacaaacgt tcaagtattt agtttttatc gtctggcttg
      361 gtatttgctg caacagacaa ctcttcttac aggaaatgaa ttatcagaat caggatctgc
      421 aatgatcttg cgacagattt tagaaaaaaa tgaggaagaa ttaacaatct ttcgcggaga
      481 aatcaataaa agagggttta ttcgtcaatt acaagaactc tacagtgaat tgcgtacagg
      541 aaatattcaa ccagaagacc tgatgctttt atttggtgat tcacctaatg cagaagacca
      601 gcaacttaaa atgaaagact tgaagctgat tttttcagct tatgacagcg aattgactca
      661 acgtgcacta acaaatgaag atgcactatc tatactagca aattatatgc agacacaaga
      721 tttcaacaac gtcaaattta ttgtcagtgg tttttctcga atcaatggtc aagagtatca
      781 attactgcag gcaatgatgg aaaaggggca attggttgtc gatttattgc tggatcgtcc
      841 ttatacagcg gatcttccag aagtacttac gctctttcat gagactggaa agctttattt
      901 ccgtcttttt caaagtgcaa gagaacggaa agtccctata ttagtggacc gatttgcacc
      961 agagcagact cgtccgacag agcttcaact gctgcaaaca atgtgggaag agacatctag
     1021 ccaaaagaaa gcggcagtac acaaaatagg gggacaagag catctccatt tgtgggaagc
     1081 tgaaaatcca actgaagaag tccgacaatt agctgttgaa atcagacgac tagtcagtga
     1141 a
//
LOCUS       DACEOE010000347         1132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.361, whole genome shotgun sequence.
ACCESSION   DACEOE010000347 DACEOE010000000
VERSION     DACEOE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.361"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1004
                     /locus_tag="IW774_002855"
     CDS             <1..1004
                     /locus_tag="IW774_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAQ1155165.1"
                     /translation="TFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSD
                     GTVTRILREATKHYQPRMNFLPTVLCMDEFKSMNSVSGSMSFICVDGTTNQLFTILED
                     RRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINR
                     SFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQ
                     TEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIF
                     HCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          349 a          245 c          219 g          319 t
ORIGIN      
        1 ggaccttttc tgcgaaaacg gacttagttg atgaacatca tcaactaaca aaggaactga
       61 agcaggcgat tctcatggaa ctgtacgaaa atcaatcacg taaactaatc gctaagaagt
      121 acttcgtctc ggatgggacg gtgacacgga tcttgcgtga agcaacgaag cattaccagc
      181 cacgtatgaa cttccttccg acggtgttgt gtatggatga gttcaaatca atgaattctg
      241 tttctggttc catgagtttt atttgtgttg atgggacaac caatcagcta tttacgatcc
      301 ttgaagatcg cagactttac aaactaactc aatacttcct gcgctttcct agaaaggccc
      361 gtttaaaggt caagtaccta gtgatggaca tgaatgccag ctattgtcag ttgcttaaaa
      421 cagtctttcc aaatgcggag atcgtaactg accgctttca tattgtccaa cacatcaatc
      481 gcagcttcaa tcaattacgt gtccagatca tgaatcgttt tcgaacctca cactctgaaa
      541 atcagaagaa gtaccgtcgg ctcaagcgat attggaaact cttactcaaa gattccacga
      601 cattggagcc gctcaaacgg cattatcatc gactgtttaa acgaccaatt agtcaaacag
      661 aaatcgttga tgagctgtta agctacaacg aagaattgcg aactgcgtat catttttgtc
      721 aattattacg ctattatttc gttaaacgag gtacggaagg ctttcaagaa acccttaaaa
      781 caatttcatc tacgttacct cagtcgttta aaaagaaatt cacgatcttc cattgctacc
      841 aatcgggtat ttcaaatgcc tttaaggtgt cccactcaaa cggcgtcact gaggggctga
      901 ataacaagat caagctgatt aaacgaattg cgtttggtta tcgcaacttt tataactttc
      961 gggcacggat ctatttacaa caaggtctga tctttgaaaa ctaaaaaaat atgcggcgca
     1021 cagaacgcgc cgcatatcgg actaagtttt attcaaattt gtcttcacca acacgatttg
     1081 acgaagagcc aaaaaaagtt acgacagttc tatgtcgtag ctcttttttt cg
//
LOCUS       DACEOE010000348         1116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.362, whole genome shotgun sequence.
ACCESSION   DACEOE010000348 DACEOE010000000
VERSION     DACEOE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.362"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(473..697)
                     /locus_tag="IW774_002856"
     CDS             complement(473..697)
                     /locus_tag="IW774_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155166.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            900..>1116
                     /locus_tag="IW774_002857"
     CDS             900..>1116
                     /locus_tag="IW774_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1155167.1"
                     /translation="MKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSHY
                     LNQLNDEQLLVKINSRPVIYFHKEAFEK"
BASE COUNT          405 a          153 c          205 g          353 t
ORIGIN      
        1 caatcttttt tgctacgtaa tttttgtaaa aaagtgggtt gagagaaatc taattgattt
       61 tcaaagaagg agtatgaaaa tgagaaaaat atttattcgt ttattgtggg tactatctta
      121 gaggaataaa taatagtgga gaaaactatg gtttggcgca gattctatca ggtttgatgg
      181 aacgaagaca acctcccgac atcaagtcaa aagctacttg agattttgat ctgaaaataa
      241 aaaaagaaaa caaggtagga acatcaatgt ttctaccttg ttttcgtaat caatgggaaa
      301 aaactaagag tggtgtacgt aatcaataaa aaggatggtg acaacagtac ctctttactc
      361 tctgaaagca tcataactcg tttttcctca ttatgccaat aaattgttgt tataccacat
      421 ataaggattt gtaatagcta aaaacagaca ggaaagttga aatctgtgaa tcttattttg
      481 tatggaagga tttatagaac gcaaaaatca agataaagat gaataaaaat cctgtttcgg
      541 ctatatattg cgtatctacg ccaataaaac gagtttgttc tgtcatagcc atataaagca
      601 aaggcaaaaa tacaactaca gcgagataag atccgatgat ttctcgaaaa gttttccagg
      661 aagtatgttt cctgttttta tatttatccg atttcattga atagtcatag cctccttatt
      721 ctttcatatg ttgaaattcc agtataaaaa ctggaaggaa ctgtacgttt tttagaataa
      781 aaattataag ggataagtag gctgctgtct ataataagat atatttttca attctttatt
      841 aaagatttta ttgacaaaat gaatccgttt tctatgatgg aggaggggtg aataagttgt
      901 tgaagaagga aattttatgt tacttagaga accagacagc ctttttcgat ttagaacata
      961 tgaacgaggt ctttacagca caaaggttag ctgatcattt tggggtaaaa agaaacacag
     1021 tcagtcatta tttgaaccag ttaaatgatg agcaactact agtcaaaatc aattctcgac
     1081 cagtgattta tttccacaaa gaagcttttg aaaaac
//
LOCUS       DACEOE010000349         1108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.363, whole genome shotgun sequence.
ACCESSION   DACEOE010000349 DACEOE010000000
VERSION     DACEOE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.363"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1069
                     /locus_tag="IW774_002858"
     CDS             <1..1069
                     /locus_tag="IW774_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1155168.1"
                     /translation="AQHQVELAYISDPGHIAYFSGYESDPHERVLALFIPLEQDPFLF
                     TPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRTRYGQPGNVGIEKNALTVERFD
                     AISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAFEIGFKAVKEGVTE
                     QAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISPNELVLFDLGVVWN
                     GYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGELDQIARNVINSYG
                     YGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYIPEKVGVRIEDCVY
                     VTSDGCVPFTTTPKELLVLEK"
BASE COUNT          335 a          244 c          243 g          286 t
ORIGIN      
        1 ggcacaacac caagtcgaat tggcttatat cagcgaccct ggccacatcg cttacttttc
       61 cggatacgaa agtgatcctc atgagcgtgt actagctctt tttatccctc ttgaacaaga
      121 tcctttcctt ttcactcctg ctttggaagt tgaagatgct caaaacagta gttgggcgta
      181 tgatgtccgc ggctatttag acagcgaaaa cccttgggag atcatcggaa aagaacttcg
      241 tactcgctat ggtcagcctg gcaatgtagg gattgaaaag aacgccttga ctgtcgagcg
      301 cttcgatgct atctctgata tcttgacggg aacggattct tacactgatc taacttccat
      361 catccaacgt ctacaactga tcaaaactct agaggaaaaa gacaaactga tcgaagctgg
      421 taaatgggcc gatgttgcat ttgagattgg ctttaaagca gtgaaagaag gcgtgactga
      481 acaagcgatc attgcagaaa ttgaatacca attgaaaaaa caaggtgttt cacaaatgtc
      541 tttcgacaca ttagtccttg caggagcaaa tgctgcaagc cctcatggta caccaggaaa
      601 tacgaagatt tcaccgaatg aacttgtgtt attcgatctt ggcgttgttt ggaatggcta
      661 ttgcagtgat gctactcgta caattgctta tcaaaaacca accgacttcc aagaaaaaat
      721 ctacaacatc actcttgaag cacagcttgc cgcacaagaa gccgtccgtc caggtgtgac
      781 agcaggtgaa cttgatcaaa tcgcacgaaa cgtgatcaac agctatggct acggcgaata
      841 tttcaatcac cgtttaggtc acgggatcgg aacaaccgtc cacgaattcc cttcccttgt
      901 agaaggaaat gacttagtaa tcgaagaagg gatgtgcttc tctcttgaac caggaatcta
      961 tattcctgaa aaagtagggg tacgtatcga agactgtgta tatgtgacaa gcgatggctg
     1021 tgtgccattc acgactacac caaaagaatt acttgtttta gaaaaataag agtaaaaaag
     1081 gtgagagcta gtccgctccc accttttt
//
LOCUS       DACEOE010000350         1093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.365, whole genome shotgun sequence.
ACCESSION   DACEOE010000350 DACEOE010000000
VERSION     DACEOE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.365"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(199..>1093)
                     /gene="argS"
                     /locus_tag="IW774_002859"
     CDS             complement(199..>1093)
                     /gene="argS"
                     /locus_tag="IW774_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1155169.1"
                     /translation="IVKLLEEKHLLNEDKGAEIVDLSAYDLNPALIKKSDGATLYITR
                     DLAAALYRKRTYDFKQSLYVVGNEQSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQG
                     GKKLSTRKGKIVLLEEVLNEAIQSAKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKN
                     DRLNTFDFNLEEVVRFEGETGPYVQYTHARAVSLLEKAGFVPSETADYALNDDTSWEV
                     VKLVQKYPETVLSAGEKYEPSVIAKHAIKLAQAFNKYYAHTKILADDEQKEARLALVY
                     AVTVLLKEDLRLLGLHAPDKM"
BASE COUNT          291 a          237 c          190 g          375 t
ORIGIN      
        1 agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac agtgtttaag
       61 agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac
      121 gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc agaacgctga
      181 tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca aacgaaggtc
      241 ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt tttgttcatc
      301 atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta atttgattgc
      361 atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg tttcagggta
      421 tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat agtctgctgt
      481 ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat gtgtatattg
      541 cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa agtcaaatgt
      601 attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc
      661 tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt tagcagattg
      721 gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag ttgataattt
      781 tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag accagtcaaa
      841 gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt cattccctac
      901 tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag cagctaagtc
      961 acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat tcaggtcata
     1021 agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa
     1081 aagtttgacg att
//
LOCUS       DACEOE010000351         1075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.366, whole genome shotgun sequence.
ACCESSION   DACEOE010000351 DACEOE010000000
VERSION     DACEOE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.366"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..203
                     /locus_tag="IW774_002860"
     CDS             <1..203
                     /locus_tag="IW774_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1155170.1"
                     /translation="DLNGEMKFRFHCCQGEIGHHHHFICTVCGTTKEIHMCPMNYFEE
                     QLGGCTIEGHRFEIFGRCENCQ"
     gene            365..541
                     /locus_tag="IW774_002861"
     CDS             365..541
                     /locus_tag="IW774_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1155171.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            568..1014
                     /locus_tag="IW774_002862"
     CDS             568..1014
                     /locus_tag="IW774_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1155172.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT          396 a          164 c          231 g          284 t
ORIGIN      
        1 ccgatttaaa tggagaaatg aagtttcgtt ttcattgctg tcagggggaa atcggccatc
       61 accatcattt tatctgtaca gtgtgcggaa caacgaaaga aatccatatg tgtccgatga
      121 attatttcga agaacagcta ggtggatgca cgatcgaagg gcatagattc gagattttcg
      181 gaagatgtga aaattgtcaa taagggttta aataaaataa catttgataa ttttaaaaaa
      241 aagcaagatt tccttgacgg tcaaaacagc attagatata atatttacag gacagtcttg
      301 ttttttgaga tatatttcaa taacaattac ggttactttt taagctcgga gggagggaat
      361 taacatgtca aaaacagtcg ttcgcaaaaa cgaatcactt gacgatgctc ttcgtcgctt
      421 caaacgttcc gtgtcaaaag cgggtacttt acaagaatct cgtaaacgtg aattctacga
      481 aaaaccaagt gtaagacgca agaaaaaatc tgaagcagct agaaaacgca aaaaattcta
      541 gttctgtgat gaattggagt tggtagtgtg tcactactaa gtagattaaa cgatgacatg
      601 aaagcagcga tgaaagcgaa agataaggaa tctttacaag tcatccgaat gatcaagtca
      661 tctatacaaa acgagcagat taaagaaggt cacgacttga cagaagaaga agaactaacg
      721 gtgttgtctc gcgaaatgaa acagcgacgt gattctttgc atgagtttga agaagcagga
      781 cgtgacgatt tagctgaaaa agtaaaaagt gaaattgtaa tagtcgaaaa atatatgccg
      841 gaacaacttt ccgatgaaga aattcgacaa cttgttcaag aagcaatcac tcaaactggt
      901 gcttcatcaa tgaaagaatt tggcaaagtt atgggtgcaa ttatgcctaa agtcaaagga
      961 aaagcagatg gaaaccaagt gaatgcgatt gtgaaagaac tattacaatc ttaattttat
     1021 catttaaact gcggacagtg attgtccgca gttttttgtg ttcgtaaaaa aagaa
//
LOCUS       DACEOE010000352         1068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.367, whole genome shotgun sequence.
ACCESSION   DACEOE010000352 DACEOE010000000
VERSION     DACEOE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.367"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..809
                     /locus_tag="IW774_002863"
     CDS             <1..809
                     /locus_tag="IW774_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1155173.1"
                     /translation="YTPQTMSNITKSFTEEVTAFNGRELHDRYAAIYMDATYIPLKRK
                     TVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERGLKNVLLFITDGL
                     KGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKEVALEA
                     RGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIESFNKQIKKYSHR
                     KEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQLIEN"
     gene            943..>1068
                     /locus_tag="IW774_002864"
     CDS             943..>1068
                     /locus_tag="IW774_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1155174.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTA"
BASE COUNT          343 a          204 c          216 g          305 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaatataa caaaatcatt tacagaagag gtaacggcgt
       61 ttaatgggcg ggagcttcat gaccgttatg ctgctattta tatggacgca acgtatattc
      121 cgttaaagcg gaaaaccgtc gccaaggaag ctattcatat cgcagttggc attcgcccgg
      181 acggatcaaa ggaagtattg agctatgcga ttgcaccgac tgaatccatc acgatttggg
      241 aggaaatttt attggacctt caagagcgcg gtttgaaaaa tgtcctcctg ttcatcacgg
      301 atggcttaaa ggggatggta ggagcgatta gtcggttcta tcccaaagct cgttttcaac
      361 attgttgtgt acacgtttcc cgtaatatca gtcataaagt gcgtgtcgat gatcgtaagg
      421 aagtctgtga tgattttaaa atggtgtatc aagcctcatc taaagaggtg gcgttggaag
      481 cacgtggtac ttttgcggaa aaatggaaaa ccagctatcc aaaagtggtt gaatcgattc
      541 tttcgaacga tcacttgctc actttctatg atttcccctt ggccatacgc aagagtattt
      601 attctacgaa cttgattgaa tcctttaata aacaaatcaa gaaatacagc caccgcaagg
      661 aacagttcca aaatgaagag tcgatggaac gtttcttagt ctcgtctttt gatacttaca
      721 accaaaaatt cctaggtcgc agtcataaag gtttccaaca ggccgaaggc gagctcgaac
      781 aaatgctaag ccaactgatt gagaattaga aacaatctgc agtagggaag aatcatttac
      841 acaaaattat tgacgctccc ttataatgag aacttttact aacataattt atgataaact
      901 tactatataa tcatattaat taaatagtta ggagatctgc ttatgtttat atctactctt
      961 caaattcgaa attttagaaa ttatgaatcc gaaatctttc gattcgataa tgagacaaat
     1021 actattatag gtgagaatga ttcagggaaa accaatgctc ttactgca
//
LOCUS       DACEOE010000353         1068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.368, whole genome shotgun sequence.
ACCESSION   DACEOE010000353 DACEOE010000000
VERSION     DACEOE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.368"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            42..1028
                     /locus_tag="IW774_002865"
     CDS             42..1028
                     /locus_tag="IW774_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1155175.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT          413 a          171 c          245 g          239 t
ORIGIN      
        1 caatatacca atcataaatc tatttgctag gaggaattta catggaagag aaaaatttaa
       61 cggtcatcgg aagctatagt acgagggaag aagcattatc agtaattgaa cggttgcgta
      121 acgaagggta tgaacgagat gatatcgtta tttatactac tgatgaagca gcatctagac
      181 taggatttga cggattgtct ggtatagatg ttgaaacaga tgaaaatcgg atggatggtg
      241 aagaagaccg ttctttatgg gaaaaaataa aagatacttt ttcttttgat acctatgatt
      301 ctgagacggc tacaccagaa aatgatcctt tatatcaata tagaggagat atttctgatg
      361 gaaaatttgt catcacagta aaaggctatc gtcagccaga aacaacagaa gatacaatgg
      421 atacacaaga tactgttagc ccagcaagca caatggggac tggattagaa actggcactg
      481 gaacagatcc aatggacgta ggtggcacga caggctttca aaatacaacg gatccattga
      541 atatgacacc aggaactgat ccgatggacg tacctggcga gactgataca atgggtacaa
      601 gcaagactga agacatcgat cgcaaaccaa atctagatga tgatacaatt caactaaaag
      661 aagaaaaatt agatgtaaat acacatgatg tgacaactgg agaagtcgac atccacaaac
      721 atgtggtcaa cgatactgaa acggtagaag taccggtcaa acgtgaagaa atcgtcatcg
      781 aacgcaaacc agtaacagat caatcttcac agggaacaga cgaaaaattg gaagatgaca
      841 cgattactat cccaatcaaa gaagagcaag tagatgtaag caaacataca gttattcgtg
      901 aagaagttgg gattcataaa gaagaacatg aagatgttga aaaagtgaca gaagatgtta
      961 gccgagaaga attggatatt gacacaagcg gtgatgtaca cattgaagac cgcaataaaa
     1021 aatcgtaagc gtaagctaaa gaggttgtga cagaagtggt cagcttaa
//
LOCUS       DACEOE010000354         1066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.369, whole genome shotgun sequence.
ACCESSION   DACEOE010000354 DACEOE010000000
VERSION     DACEOE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.369"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(22..900)
                     /locus_tag="IW774_002866"
     CDS             complement(22..900)
                     /locus_tag="IW774_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155176.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT          336 a          205 c          169 g          356 t
ORIGIN      
        1 aactatttcg aagtactaaa cttatgaagt aagaatacct aagccagagc caatgtgctc
       61 gcctgcaagc aattgctctt ttgcttgtcg ctgtaaactc gtactttctg gataatcatg
      121 tatttgggca tctactcgtc caaagatcca agggactcca ctatcgtcct gttcatcaac
      181 tgataataat cgataatcgg ctgcaaaaag acagattttt gactcttttt tcaaccattt
      241 agtaagattg ggtgatagta accaagtatc tgtgtacatc acttggtaat ggtaatcagg
      301 aaaataaagt tcaaaaaaac gaatggcact ttcgtaacta tctcttatct tattctgaga
      361 taaatctgca tcggaaggaa tatgaagaga aaggcactgc tttccttcta atcttagatc
      421 cgtgtaccct ttcggaaaat atgtcatttc gtactccaat tcaccaatcc gaaagatcaa
      481 gccactagtg taatgccaaa tccaaaaacc acggtcaaag cgatagcttc ctgttacttg
      541 gaaatgttca tgaataaatc tagtaaaagc attcattgtt gccaaataga tagttcttgg
      601 aatcttcaat ggttgataaa tttttttcca actttgagaa gctgcatata gataaatagc
      661 taatgctgca aaaggttcct cttcatagac tttgtctatt ttctttgcag tcgcctttgc
      721 tttattagga tccattagtt catgactata ttgctcaaac acctcatcaa ggatgttttt
      781 tgcttgtaca atcatttgct ctataacgac tttgggaaga gttaaagttt ctgccagaat
      841 ttggatttca ttgcttattt tcttcatcaa atttcccctt ttcatatctt tgaacctcat
      901 tgtttaaatg actgattatc cggacaccat atggtataaa catactttta ttgtacacta
      961 actataaaac ttaaactttc tatgaagaaa gaatatctgt aagactttca aggaaagcat
     1021 tgaccatctc aggaaagttt attcagttac catcataaac aaaatt
//
LOCUS       DACEOE010000355         1065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.370, whole genome shotgun sequence.
ACCESSION   DACEOE010000355 DACEOE010000000
VERSION     DACEOE010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.370"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1028
                     /locus_tag="IW774_002867"
     CDS             <1..1028
                     /locus_tag="IW774_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1155177.1"
                     /translation="YAENLSITPDLSNVPLHKENTYTVKELFDSAIIQSANASMVALA
                     EKISGSEAKFTERMKEQLKDWGIKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQ
                     DVAIVARHLILDFPEILDVSSTTTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLK
                     TGTTELAGACFVGTITKNDQRIITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKE
                     LGKANASIPSLKTIDVKDGKESTVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTD
                     NEIQAPMDKGTKIGTATITLADDKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVA
                     EFFGNLF"
BASE COUNT          367 a          215 c          234 g          249 t
ORIGIN      
        1 attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg cataaagaaa
       61 acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc aatgcttcaa
      121 tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa cgaatgaaag
      181 aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca ggattgaaca
      241 actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa aatcaaatgt
      301 ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca gaaatactgg
      361 atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca gtggaaatgg
      421 tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg gacggattaa
      481 aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact aaaaacgatc
      541 aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg agtgcccgct
      601 tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa aaagagctcg
      661 gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac ggaaaagaat
      721 caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac gatatggata
      781 ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac aatgagatcc
      841 aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg gcagacgata
      901 aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg gataaaacag
      961 tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt ttcggcaact
     1021 tgttttaatt gaacaataag ggctctttgt caataaggac tgatg
//
LOCUS       DACEOE010000356         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.371, whole genome shotgun sequence.
ACCESSION   DACEOE010000356 DACEOE010000000
VERSION     DACEOE010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.371"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1062
                     /locus_tag="IW774_002868"
     CDS             <1..>1062
                     /locus_tag="IW774_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1155178.1"
                     /translation="RLLIPSAKIVPEELHHLGKLPAIIYPINQKIVFDYLYDQYKDVC
                     SAIDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCDEPVIINFA
                     DTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNELKEDDGKKHKLFS
                     GVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNWFDIGHADK
                     YYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVEYVRPRIFE
                     YSTSYINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCSDFKRYSVSGDNI
                     QKSLKDMYLDKTFQRFNKL"
BASE COUNT          384 a          132 c          189 g          357 t
ORIGIN      
        1 tgagattatt gattccatct gctaagattg taccggaaga acttcaccat cttggaaaac
       61 ttcctgcaat tatctatccg attaatcaaa aaattgtatt tgattattta tatgatcaat
      121 ataaagatgt atgttctgct atcgatattg cctgttacga aaagatggat aaggtagcaa
      181 gacgattaga taagtatatt aaatctaaaa cagtaaatat tattcagctt aaagagttag
      241 gggatcttgg aagaactata tatgattctc taataggatg tgacgagcct gttattatta
      301 attttgctga taccattatc aatgataata tatattcctt ggaatgtgac agctttttct
      361 atgctgaaga ctattatagt aatacttgga cattctttga agaaaaagac ggagatatta
      421 tttctgtttt agataagaac gagttaaaag aagatgatgg aaagaagcac aaactgtttt
      481 ctggtgtatt tcaaattatg gatgcccagt attttagaga gtgtttgcgt aaagctttga
      541 tgtcaaatgt agtaaatgtg aatagttttt atcaagcttt acaagagtat agcaaacgat
      601 atgaatttct ctctattaaa acaaataatt ggtttgacat tggacatgca gataaatatt
      661 ataattccaa attggaagtt aaggcaagag aatttaacca catttcaatt gataaagatc
      721 gtagcatcct gagaaaaatt agtgaagatg ttgaaaaatt tatcggagaa attaagtggt
      781 atcttaagtt gccagcacag gtagaatatg tgagacctag aatattcgaa tattctactt
      841 cgtatattaa tccatatgta agtatggaat actattcgta tcatactgtt cacgagttat
      901 ttctctatag tgacttaaca aaaaaacaat ggatagatat ctttaataga atacgatttg
      961 tatgctcaga ctttaagaga tactctgttt caggggataa tattcaaaag tcattgaaag
     1021 atatgtatct cgataaaaca tttcagagat tcaataagct ca
//
LOCUS       DACEOE010000357         1018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.374, whole genome shotgun sequence.
ACCESSION   DACEOE010000357 DACEOE010000000
VERSION     DACEOE010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.374"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            452..565
                     /locus_tag="IW774_002869"
     CDS             452..565
                     /locus_tag="IW774_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HAQ1155179.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
     gene            complement(653..946)
                     /locus_tag="IW774_002870"
     CDS             complement(653..946)
                     /locus_tag="IW774_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1155180.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          314 a          167 c          156 g          381 t
ORIGIN      
        1 aaaaaaaaga taaaacttga ttaattgaag tattgcttaa atttggaagc tatgagggaa
       61 gaataaaaga gttaggacgg gattatctac tatttttctt tgttttaaat atttgttatc
      121 tttgaacata aaagagaaat gtgtcgactg cagtgttgtg tccctcatca cgaataaacg
      181 gtgttcaatt agctgatctt gtattcactt aaaaaatggt aaagtacgag ttgctattcc
      241 ccattttcaa ttttctaacg ctcaggacaa aatactgttt tcacaacctc attggaataa
      301 aagcatatat attgtttcaa gaacgaaatc ttgttatctt tttaaagggc atctgtccaa
      361 aatctaattt taggaggtac gttttggtat ttattcgtac aaataaaaga gaattatctt
      421 tattcatgta acgcatccaa ttgatttttt catgtacgct cctggaacga ttttactgac
      481 gattgcttat ttaactgcga atcgctcatt agcttttatc atagctatcc atattttgaa
      541 taacgttctt ggctttgtat tatagataca gatgacaaaa aaagttcaag tatgcggtgt
      601 agaaccaata tcgcatactt gaactttttt caatgagtat aaattaaaac ttctaatttt
      661 caattcccat catctgatat gcccgaccgt ctgaacttct tttgaaaaag ccaaatgtga
      721 ttagataacg tctttgttcg acataatcgc cactagaaaa tacgttcagc agtatcgaat
      781 taacctctgt ttctgtataa gtcactccat ggtcaaagcg tttcaagact tctttcaata
      841 ggatgtattt cttttttgtt gaacttggta cttgttttac ttttccatca ttcatgaatg
      901 tgtctaaaac tttcatcttt tcttgctccg tataagtata aaccatcttc tttctccttt
      961 tgattctaag aatactccat ttatgttaga atattctgga ttttttatta aagcggtt
//
LOCUS       DACEOE010000358         1004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.376, whole genome shotgun sequence.
ACCESSION   DACEOE010000358 DACEOE010000000
VERSION     DACEOE010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.376"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..210)
                     /locus_tag="IW774_002871"
     CDS             complement(<1..210)
                     /locus_tag="IW774_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1155181.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="IW774_002872"
     CDS             complement(309..623)
                     /locus_tag="IW774_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155182.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(691..981)
                     /locus_tag="IW774_002873"
     CDS             complement(691..981)
                     /locus_tag="IW774_002873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155183.1"
                     /translation="MTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQWSIENKDGEEL
                     YEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGLPFTL"
BASE COUNT          319 a          192 c          112 g          381 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttggta gtcctgcctt tcaatcggta attcataata
      781 gtaatttact gtttcttctt tgccactttc aatatactct ggattatcca atatcatatc
      841 atactcataa agttcttcac catccttatt ttcaatcgac cattgttgtg gacgtatcgg
      901 attttctgta tcatttataa gtgtataggt cacttgtaat acacgatcat gttgtgcaaa
      961 ttcatttctt ttttcggtca cttcaatatt tttaaatgtt agtt
//
LOCUS       DACEOE010000359          996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.378, whole genome shotgun sequence.
ACCESSION   DACEOE010000359 DACEOE010000000
VERSION     DACEOE010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.378"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..963
                     /locus_tag="IW774_002874"
     CDS             4..963
                     /locus_tag="IW774_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1155184.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          340 a          182 c          206 g          268 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataact
//
LOCUS       DACEOE010000360          992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.379, whole genome shotgun sequence.
ACCESSION   DACEOE010000360 DACEOE010000000
VERSION     DACEOE010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.379"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            378..539
                     /locus_tag="IW774_002875"
                     /pseudo
     CDS             378..539
                     /locus_tag="IW774_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            689..889
                     /locus_tag="IW774_002876"
     CDS             689..889
                     /locus_tag="IW774_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1155185.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT          329 a          146 c          207 g          310 t
ORIGIN      
        1 atttaatcta tttgataaaa aataaaattc aataatggta taataaaatg tcggctcgtc
       61 gcttccacag agattttcgt gggaggaggt gtatgatttg tttgaattca ctttggagca
      121 cgtgctcttg ccattgttaa ttggtttaac tttggcatta tttagtcatt ggctagataa
      181 acattagagc cgacaatggc gcttactgtt tcgcagcggt aagcgtcttt ttttgaaaaa
      241 gaaaaaaaca caacgtattc gcaggtacgt tgtgtttatg gtgtatattt gtttaaattc
      301 actttcaatt attattatac ttgttaaata aattgtgtca atataaaaga tatttttata
      361 cgtgtactaa cctatattat ccttctggta atgttattcc atcgcagcag gatatgaatt
      421 ttaccaaaag gatacaaaag tgtggagaaa tgatggggat cactgtactg gaccacttga
      481 tcatcggacg caaacgatat tttagtttga gagaagaagg aatgatggaa gagaagtaag
      541 ttgctttctt gcaaaaaaaa tgaagcaagt gtacaattat attgtaattt tgtttgggaa
      601 tagaagttga aacatctgtt gcacctctat cgtacacgag caatgttaca atttaaatta
      661 gtgcttttgg cacgaggagg atattattat ggaaaacgga acagtaaaat ggttcaacgc
      721 tgaaaaagga tttggattta tctcacgtga agatggaagc gacgtattcg ttcacttctc
      781 agctatccaa ggtgacggat tcaaaacatt agaagaaggt caagcagtga ctttcgacgt
      841 tgaagaatca gaccgcggcc cacaagctgc aaacgttgta aaagcttaat tttatctgta
      901 aatcaaattg atggtagaca tcaattttta cgagagaaca aagcgagtaa caaaaatacc
      961 tgccacaaga gacaatttct cttgtggcag gt
//
LOCUS       DACEOE010000361          984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.380, whole genome shotgun sequence.
ACCESSION   DACEOE010000361 DACEOE010000000
VERSION     DACEOE010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.380"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            70..549
                     /locus_tag="IW774_002877"
                     /pseudo
     CDS             70..549
                     /locus_tag="IW774_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT          318 a          172 c          228 g          266 t
ORIGIN      
        1 ggcttattac cgcagataca cggctgtttc tggattacca tttattctgg ataagggggt
       61 gtggcacgat tttcagaagg aatctatgaa ccaatcactg tattgatttt agcttcgatg
      121 gtaaccccat tcggcttgac gatcgcttat ttcttaggga aaatcattcg taaaaatata
      181 ctcaatcgtc aagaaatcga tacattgaag accgcttttc ctatggggat ctgtcagatc
      241 acagagggat gtttccctat cgtattgaac gacttggcac gcaatgtcat agcaacggga
      301 gtcggtggga cagtaggtgg cggattaagc atgttttggg gcgcagatag ccgtattccg
      361 gcatcgggaa tgtttgcagt cgctacgatg accagaccat gggcattcat tggtgcattg
      421 ttagctggtt cttttgtcac aggaataacc attttattat tgaaaaaacc agtagatccg
      481 aatgcagaaa tcatccaaga agaaaaagag gaagaagaca tatcttggga tgatttgaca
      541 attagttagg agaggcagaa tggattacaa gaaaaaagta caatagatgt accttgagac
      601 aggaatggta tgaaagaatc agagttagaa agaatcgaat atacagattt tggtttagga
      661 gcgatcgaaa ctgtccagag catcggcacc ctccttgaaa tgacgatgaa ggaaaaatga
      721 gaacctttcg ttgtcgaaag ggtctggtat accttatgtc gaaggaaagg aaacaaaaaa
      781 actttcctgt actattccaa aaaggaaaga aagttattat acagtaaaaa aagaaattat
      841 tcttcatgct agagatcagt acacgttcga gcccaatatc aaacactagt tccaggcagg
      901 cgcaacaggt gcggttatca ctgaattttc ttccagcagt gacgattcaa gtgatatttt
      961 tacaaatcca gctatttatc gtta
//
LOCUS       DACEOE010000362          974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.382, whole genome shotgun sequence.
ACCESSION   DACEOE010000362 DACEOE010000000
VERSION     DACEOE010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.382"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            94..969
                     /locus_tag="IW774_002878"
     CDS             94..969
                     /locus_tag="IW774_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1155186.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSDDKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGA
                     QDFHDKRWYLNIPQR"
BASE COUNT          342 a          162 c          197 g          273 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa ctacttcaga tgataaacca gtcattgatc caactagagc aggtgcagca
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg agaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaatt ataaatacga gtatattttc
      721 attatggatt acaatactgg aaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa atcaagctta taatacttct ggaaacgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctaataa gaaagtctat gtaatgatgc gtgcaacgaa tgatccagaa
      901 gggaacacta aaggcggagc acaagatttt catgataaac gctggtattt aaatattccg
      961 caacgataaa aaaa
//
LOCUS       DACEOE010000363          972 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.383, whole genome shotgun sequence.
ACCESSION   DACEOE010000363 DACEOE010000000
VERSION     DACEOE010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.383"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..154)
                     /locus_tag="IW774_002879"
     CDS             complement(<1..154)
                     /locus_tag="IW774_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1155187.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLL"
     gene            complement(228..767)
                     /locus_tag="IW774_002880"
     CDS             complement(228..767)
                     /locus_tag="IW774_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ1155188.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT          258 a          203 c          163 g          348 t
ORIGIN      
        1 ccagcaaagt tttgatcgtt gctggtttcc aatccatcga atcagctaag atctgcgtga
       61 tttcttgggc tgtcgtctgt ccaagtgtcc aaataacacg catgatctcc cactctgaat
      121 cactgatctt tactggcgtt ttgacttttt ccatttattt cacacctttc gtttacaaat
      181 gtaatcatta gcttatgaat acagattacg cttgtaaaca aaaactgtca attatttttg
      241 ctctctattt tcaatttagt taaaaattct tgtttctctc gtcttgtttt aaggatgatc
      301 agtttatttc ttgcattagc tacttggatc agctgctgta ttttcggttc attgttttct
      361 tcaaaatgcc agataaaaga taaaaattct aagtattccc tgtttagttt ctctgaaaat
      421 gattcaggca tatctggccg agattttttg tttatctttc tttttagaga acgccaaact
      481 actcggtaga tggcgagata acgcggcact ttaagccaaa tgatcttatc tgcttcttta
      541 atcctttcct ctaatgtaga agtataattt ccttcaacaa tccatgacgg attagatacc
      601 ataaacgatt gctgttcttg caaaaaccac ttttttgcct gaatactgta gtctgttgta
      661 tgccataaaa ggtctagcgg cagtagcgga aagtcggtga tttctttgat cttccttgcg
      721 agggtcgatt ttcccgaccc actttgtcca atcacgatga tcttcattct tctccctcac
      781 ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa caagggactg
      841 tgacaaaagt cgtgtcaaag tcccttgttt cgaataaatg gtgttcaatc agctgacctt
      901 cagcattaac tcaaaaaacg gcaaaatatg aggagctatt ttttcgtttg ttcctctaat
      961 actcaggtca aa
//
LOCUS       DACEOE010000364          957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.384, whole genome shotgun sequence.
ACCESSION   DACEOE010000364 DACEOE010000000
VERSION     DACEOE010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.384"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..244
                     /locus_tag="IW774_002881"
     CDS             <1..244
                     /locus_tag="IW774_002881"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="catalyzes the addition and repair of the
                     3'-terminal CCA sequence in tRNA; these proteins belong to
                     the CCA-adding enzyme subfamily 2 which does not have
                     phosphohydrolase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1155189.1"
                     /translation="MIYFDETPQMDAVGESYDRLPIKSLSELAVDGKILLEETGKKPG
                     KWVSEALQLAEKAVVEQRIKNDKKQVLYFLSKEKLI"
     gene            398..853
                     /locus_tag="IW774_002882"
     CDS             398..853
                     /locus_tag="IW774_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155190.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
BASE COUNT          349 a          153 c          199 g          256 t
ORIGIN      
        1 gatgatctat tttgacgaaa cacctcagat ggatgccgta ggagaatcct atgatcgttt
       61 acctatcaag tcattaagcg aattagcagt agatggtaaa atcttgctag aagaaactgg
      121 aaaaaaacct ggtaagtggg ttagtgaagc tttgcaatta gctgaaaaag ccgtcgtaga
      181 acaaagaata aaaaatgata aaaaacaggt tttgtatttt ttatcgaaag aaaaattgat
      241 ttaaaaaaca ctagactgta tctgctcagc tttgttggct acttaaagtg ggcagatacg
      301 tcattagaag aaaaaacata agatgtgacc acttacataa atttgtgcta aactgaggta
      361 gtacaagaat aaattacatc ataaaggagt ctttttaatg agtaaagaaa tgacagcgct
      421 aaaattttat tttagaaatg gagaaacctg gacgatcaac cgccgtcata tcggcgatct
      481 ttggatcaaa caaatcacaa caagttttgg acgaatcaat ggcagtgaat ttgttgaaat
      541 ccatccgtgt gctggattta aaatcgaaat cttccatgaa ggggacgcag ttgcgacaca
      601 tgatatcaac ctaggcggac tggaaatggg catgtttaat cgcgcattga aatacgaaga
      661 tatcgagcga atggaaatcc tttaccgaaa tggcacacct gatcttgtgt acttcccata
      721 tctagataaa ggaacagaag gattagacaa tcaatatcaa tcaacaaaaa tcagcgaaaa
      781 aacaggtaac ttgtatatcg tgatcaatcc agatcaacgt gtagaagatg tatacgggga
      841 atttttcgaa taaatcgatt tttacgcaaa aatgaggctg tgaaagagat gtgtcttatt
      901 actactgata ggctgtctct tccacaccgt ttattcagaa taagggactg tgacaag
//
LOCUS       DACEOE010000365          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.385, whole genome shotgun sequence.
ACCESSION   DACEOE010000365 DACEOE010000000
VERSION     DACEOE010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.385"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>943
                     /locus_tag="IW774_002883"
     rRNA            2..>943
                     /locus_tag="IW774_002883"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACEOE010000366          931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.386, whole genome shotgun sequence.
ACCESSION   DACEOE010000366 DACEOE010000000
VERSION     DACEOE010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.386"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            7..>931
                     /locus_tag="IW774_002884"
     CDS             7..>931
                     /locus_tag="IW774_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1155191.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFR"
BASE COUNT          312 a          145 c          154 g          320 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc t
//
LOCUS       DACEOE010000367          923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.388, whole genome shotgun sequence.
ACCESSION   DACEOE010000367 DACEOE010000000
VERSION     DACEOE010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.388"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            248..781
                     /locus_tag="IW774_002885"
     CDS             248..781
                     /locus_tag="IW774_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1155192.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          300 a          174 c          164 g          285 t
ORIGIN      
        1 gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt ttttgaaaat ggttcctcac
       61 aactttgcat aaatttgact taataccgag aagtcatgat ccccatctaa ctccaaatca
      121 caaggaatga gttcaaaaac tagcttttct caacaaatat tacataaaaa attctatgtt
      181 ttttgttaaa tttatttgct tttttcaaaa gtatgttaca gtaatttccc aaggaggttt
      241 tacagaaatg aattttgttg ccatggattt tgaaactgcc aatcatcagc cttatagtgc
      301 ttgttctctt gcgctagtca tggtaaaaaa tagccagatc gttgatgaat tctatacatt
      361 gatacagcct gagactcctt ttttctggcg aaatgtccaa attcacggaa tccatcaaga
      421 agacgtacgc aatgcgccga aattcccaga agtctggcaa actatccaac catattttca
      481 aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat acaaaggtct tagcgggatg
      541 cttagattat tatcatcttg aacagcctaa ttatctttct ttatgtacag tgaaaagtag
      601 ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa
      661 catccaatta gatcatcacc atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct
      721 gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa aaacttgtga ctataaagta
      781 aaagaatact tatgaaacac acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca
      841 aggagcaatt tcaaaaaata gcttctcatg tttattaaat attgtgactg gtgaccgagg
      901 agctggcagg agaacaccgt tta
//
LOCUS       DACEOE010000368          913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.390, whole genome shotgun sequence.
ACCESSION   DACEOE010000368 DACEOE010000000
VERSION     DACEOE010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.390"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(7..756)
                     /locus_tag="IW774_002886"
     CDS             complement(7..756)
                     /locus_tag="IW774_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1155193.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT          310 a          165 c          164 g          274 t
ORIGIN      
        1 gtttttttat gcgtgttgtt gtgcgttgta gtattcttct tccatttttt gcatacgttc
       61 acgttctttt tggttatacc aaggtgaaac ggtaaatgca aggacatcat tcagcaagta
      121 aacagagctg ttcacgaaca tcgccaaact tgcagatcca tgcgtaaatg aaatatacca
      181 aaggaccatt tgagcaagcc ctgaagctaa ccaccagtaa tattggttgt tgtaacgcaa
      241 gaaactgatg ataccagcag tcaagctaat agaaaaagca atggcatcta cccacggccg
      301 aggatcatct gtataacgac caatcaggaa tcctgaaaca agatagacaa ggaaagtact
      361 gatgattgcg ataaaccagc tttttccagt gaattttcgg atttttgaag ccatattatg
      421 attccattct ttacttaaca atacggggat atccaatgtg atcatataag caatctgttc
      481 accgatactt aaatagttct ttgctgaaaa cccaacgata ataaaacaaa tcgcagacaa
      541 aatacctaaa ataccgttga ctgattttgc cgcattgatg gataaaatac acaatacacc
      601 aagcgtggta ccgataaatg taataacgga cagccaagtg atgggactat tgacaagagt
      661 catgacctgg cagccaagac tgaaaaagaa aagataataa ttttgttgcg gccagccttt
      721 tagctgatga gctaaaaact gaaaatatga cttcatgtat gaggactcct taagacacta
      781 aatgtagtgt gttctgtatt tactaacaca atctattgta tgcttttctt gaaaaaaaac
      841 tagtctttca attataaata atttgtgtta tgataatttt ctgtttgttt tcttgaaaat
      901 ataatttctt act
//
LOCUS       DACEOE010000369          902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.392, whole genome shotgun sequence.
ACCESSION   DACEOE010000369 DACEOE010000000
VERSION     DACEOE010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.392"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            66..881
                     /gene="thiD"
                     /locus_tag="IW774_002887"
     CDS             66..881
                     /gene="thiD"
                     /locus_tag="IW774_002887"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1155194.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          325 a          146 c          205 g          226 t
ORIGIN      
        1 acatatgata gaaacagtaa cagtattatg aaaaatcttt ttataaaaat ggaggaatcg
       61 atttgatgga agatcagtat gtacaagcaa tgacgatcgc aggatcagat agcgatggaa
      121 gcgcagggat gcaagcagat atgcatacat tctttacaag aaatgtttac ggtgtttctg
      181 tcatgaccgc atgtgttgca ggtaattctt acggaatcgg agcaagtgtg acattgccaa
      241 cagattttat cgacaaagaa tttgaactga ttgcgaagga ctttcaaata cgtgcagcta
      301 aaacaggtat gctagcagat tcaaagttga ttgagacagt tgtaaaaaat taccaaaaat
      361 atgattttgg accattagtc gtggatcccg taatcgtaac gaaacatgga aatcttttac
      421 tagaagaatc tgctttgcaa agcttgaaag aaaagcttgt ccctttggcc gaagtactga
      481 cacctaattt ttatgaagca gaaaagctga ctggacggac attagcagct gaagaagatt
      541 ttgtgcaagc agcaaaagaa ctgcaagata tgggtgcaaa aaacgttatg atcaaaggac
      601 atcatgctaa aaatgcgcag caagaaacag tcaatgacta cgtattgttc gaatcaggaa
      661 aatcattttg gttaaatgca ccattttatc ctaccgaccg gatcaacgga actggcgatt
      721 cgctatctgc atgtattgca gctgagttag cgaaaggaaa aacagttgaa caagcgatcc
      781 gattggcaaa agactatgta aaccgtgcaa ttcgtgaaga aataaatgta gggcacaaat
      841 atggccccat caatcattgg gcagctggtg aattagagta aaagaaagaa gttgtgaaaa
      901 ag
//
LOCUS       DACEOE010000370          900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.393, whole genome shotgun sequence.
ACCESSION   DACEOE010000370 DACEOE010000000
VERSION     DACEOE010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.393"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..360
                     /locus_tag="IW774_002888"
     CDS             <1..360
                     /locus_tag="IW774_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1155195.1"
                     /translation="QEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLK
                     NLQMTEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEM
                     LDTTRFGEFIELHYVRK"
     gene            complement(439..>900)
                     /locus_tag="IW774_002889"
     CDS             complement(439..>900)
                     /locus_tag="IW774_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1155196.1"
                     /translation="GYESVFVKIDDLPFYNEDLETPEQAPAEWTRFREEVRGLDGVVF
                     VTPEYNRSVPAVLKNALDVGSRPYGHSVWDHKPGLVVTASPGGIGGFGANHHLRQSLV
                     FLNVPTLQQPEAYIGNVANLIDENGNIVEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT          285 a          178 c          179 g          258 t
ORIGIN      
        1 caggaagcaa aaaaatattt ggagaccgaa gcaatttatc cagagaaaaa gaatttcgtt
       61 ttttccaccc acgcacaatt acaattgcca gatgctgagg tcgtaaacga agatcctgtt
      121 gcttttctaa aaaatctaca aatgacagaa ggaaaagata tctgggttgt gggtggcggt
      181 cgattgatca aaccgctatt ggaacatcaa atgattgatg aatggtatat ccagattacc
      241 cctgtattat taggggacgg gattccttta ttccaaaaag gaacatatga agcgcgtttt
      301 gaaatgctag acacgacacg attcggtgaa tttatcgaat tgcattatgt acgaaagtag
      361 aagtaaataa aataaaaaag gcaactatat gcagaaatgt cctgtacata gttgcctttt
      421 ttattaccgt tatgcttttt actctgttaa tttgttagaa aaatctagat acgcatctaa
      481 aatcgtttgg aagaaactta atgttccttc aacgatatta ccattttcat caattagatt
      541 cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt ggcacattca aaaagacaag
      601 tgattggcgt agatggtggt ttgcgccaaa gccgccgatc ccacctggtg atgcagtaac
      661 gactaaacct ggtttatgat cccaaacact gtgtccataa ggtcttgagc ctacatctaa
      721 agcatttttc aatactgctg gcacagaacg gttgtattca ggagtaacga aaacgacacc
      781 gtctagtccc ctcacttcct cacgaaaacg agtccattca gcaggagcct gttctggagt
      841 ttcgagatcc tcattgtaaa atggcagatc atcgattttt acaaatacac tttcgtaccc
//
LOCUS       DACEOE010000371          898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.394, whole genome shotgun sequence.
ACCESSION   DACEOE010000371 DACEOE010000000
VERSION     DACEOE010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.394"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(65..778)
                     /locus_tag="IW774_002890"
     CDS             complement(65..778)
                     /locus_tag="IW774_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1155197.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
BASE COUNT          273 a          200 c          151 g          274 t
ORIGIN      
        1 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
       61 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
      121 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
      181 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
      241 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
      301 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
      361 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
      421 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
      481 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
      541 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
      601 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
      661 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
      721 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
      781 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
      841 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaata
//
LOCUS       DACEOE010000372          896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.395, whole genome shotgun sequence.
ACCESSION   DACEOE010000372 DACEOE010000000
VERSION     DACEOE010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.395"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            12..578
                     /locus_tag="IW774_002891"
     CDS             12..578
                     /locus_tag="IW774_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1155198.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            723..810
                     /locus_tag="IW774_002892"
     tRNA            723..810
                     /locus_tag="IW774_002892"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:755..757,aa:Ser,seq:cga)
BASE COUNT          305 a          143 c          184 g          264 t
ORIGIN      
        1 ggggagtttt aatgagaaga aaagtctata ccaaggatca aattctaaaa gctgcttatg
       61 atgttgttgc aaaggaaggg tttaaaggat ttactgcgag aaatattgct aaaaagatgg
      121 ggatttcgac acagccgatt tatctggaat tcaaaaacat ggaagattta aaaaatacgt
      181 tgctggattc tatctttgaa caactaaaag aagatgtttt ccctattgaa cgtactggtg
      241 ataaaatcat tgatctcggt ttgaactata tctactttgc aaaaaagcat catagtcttt
      301 acatggcatt gtacattgat gaacatgggg acggaaagaa aatgcatgat ttttcataca
      361 attatttccg tgaaatagtt aaagaagatg aaagatacaa tcaattatcg gatgaatatg
      421 tcgatgctct gcatactggt acatgggtag cagtgactgg cttagcgtca ttgatgtcat
      481 caaacatcat gcatccaaca gaagaacaaa tcattgcact gatcaaacgg acaattgact
      541 cgattttagc attagataat cctgaaataa tcagatagtg ttctatcagt atgaatttga
      601 tcggtaagtc cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa agcgatttct
      661 gttatttatc tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt
      721 acggaaggtt ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc ggctaatacc
      781 ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg attagaggtt gtgaagaagc
      841 tgtcctgcat caagtaataa gaacggccaa acaaaaatag cttttcctat tttttc
//
LOCUS       DACEOE010000373          852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.397, whole genome shotgun sequence.
ACCESSION   DACEOE010000373 DACEOE010000000
VERSION     DACEOE010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.397"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..382
                     /locus_tag="IW774_002893"
     CDS             <1..382
                     /locus_tag="IW774_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155199.1"
                     /translation="AFISNGESLKNVTARIRKENEVDIVFGIDHECNSVIYVTQSANL
                     ILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFVSEQKGSFSKKIGIEVIESIMCH
                     NHITLEELEILIGQKNNNTKIQVS"
BASE COUNT          302 a          120 c          145 g          285 t
ORIGIN      
        1 agcatttata agtaatggtg aaagtttgaa aaatgtaact gcgcgaatta gaaaagaaaa
       61 tgaagtagac attgtttttg gaattgacca tgaatgtaat tcagtgatat atgtaacaca
      121 atcagcaaat ttaattcttt ttgagaatgg ttgcaaggac gattttatgg tggtctattt
      181 gtacgatata ctaattccaa aacttcgttc cttatatatg tcttctgaaa gtatttttgt
      241 gagtgaacaa aagggatctt tctcgaaaaa aataggtatt gaagtaattg aatcgattat
      301 gtgtcataat catattacgc tagaagaatt agaaattttg atagggcaaa aaaataataa
      361 cacgaaaata caagtttctt agaaatcatt ttttattgag atatctagat aaatgattta
      421 ttcttcaatc aaccaactcg ccaagctgag gtaatgtttt gaggtaatga taacatacaa
      481 ctttttatac cctagtaaaa ctctataaac aaagacttat taatttcgca tgaaatacat
      541 tcctgtaaaa tgcaataaaa tagagttttg tttaaccagg tcttaagaaa gcccgtaaag
      601 cttcacagtt ctcaaaacgt taattgcgat tatcgttcga gttcacgagt tacgagaatc
      661 gtactgggcg aagcaatgct gagcgcagtt accttaatcc taacttttct ggcactttcc
      721 acaatttgga aagtgccttt tttgtgtttt tagctatgtt aataggtagg attcttggtc
      781 tataatatca ttcgatctat aaacaaacgg gaaaaagata aagtatcatc aaacaatatt
      841 atttatccgc tt
//
LOCUS       DACEOE010000374          847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.398, whole genome shotgun sequence.
ACCESSION   DACEOE010000374 DACEOE010000000
VERSION     DACEOE010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.398"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            282..521
                     /locus_tag="IW774_002894"
     CDS             282..521
                     /locus_tag="IW774_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155200.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          288 a          157 c          126 g          276 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttg
//
LOCUS       DACEOE010000375          785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.404, whole genome shotgun sequence.
ACCESSION   DACEOE010000375 DACEOE010000000
VERSION     DACEOE010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.404"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>785)
                     /locus_tag="IW774_002895"
     rRNA            complement(<1..>785)
                     /locus_tag="IW774_002895"
                     /product="16S ribosomal RNA"
BASE COUNT          156 a          253 c          172 g          204 t
ORIGIN      
        1 gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt gcgggccccc
       61 gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg cttaatgcgt
      121 tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt ttacggcgtg
      181 gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag cgtcagttac
      241 agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat ttcaccgcta
      301 cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa tgaccctccc
      361 cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg ctttacgccc
      421 aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca cgtagttagc
      481 cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc ttgttcttct
      541 ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt gctcggtcag
      601 actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt gggccgtgtc
      661 tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc cttggtgagc
      721 cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca cccgaaagcg
      781 ccttt
//
LOCUS       DACEOE010000376          778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.405, whole genome shotgun sequence.
ACCESSION   DACEOE010000376 DACEOE010000000
VERSION     DACEOE010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.405"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            23..460
                     /locus_tag="IW774_002896"
     CDS             23..460
                     /locus_tag="IW774_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155201.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            453..>778
                     /locus_tag="IW774_002897"
     CDS             453..>778
                     /locus_tag="IW774_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155202.1"
                     /translation="MNNVVYQLTMEDISRFLTNKEHLNEVIRRIQKDFSCMEEMKYFV
                     QNVEEQLYLESETSQTKVTVSNNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKS
                     ELVENF"
BASE COUNT          316 a          110 c          152 g          200 t
ORIGIN      
        1 gaagagaaag gaagataaaa tgatggctca aaattactat gacgaatttg tcaaattacc
       61 tttggataaa atggcacaaa aaatggagga tatgacgttc ctatacaacg aaacacgtgt
      121 tccaaaaaaa cattataaag aaaaattgtc ggtagctgtg gaagaaatga ttgaatcagg
      181 tgtcgaaatg aatcttattg cgacgtacta tcgaacactg gaagaactga aaaagcaaaa
      241 tgcaaaatgg tttttccaag ccttactatg cttggaagta ggtgtcaaac caagcactat
      301 taaaccttct gaataccaag ctttagaact aacgtatgcg aaatttatcg aaacgaaaaa
      361 agccaagact gtttcaagtg aatggttaga ttattttgaa aatatcaata agtatggcgc
      421 ctattacacc atgaaaaagg aggacaacga aaatgaataa tgtagtttat caactaacaa
      481 tggaagacat ttctcgtttt ttaacaaaca aagaacattt gaatgaagta atcagacgga
      541 ttcaaaaaga ctttagttgt atggaagaaa tgaagtactt tgttcaaaac gttgaagaac
      601 agttatattt ggaaagcgaa acgagtcaaa caaaggtcac agtcagcaat aatcgtttag
      661 atgtgtataa acgtcgtcct gaatcattaa aaaaaggagc ggagcttcta cgactagaaa
      721 cagaaaaata cgtcatgaat gtttacctag aaaaaagtga gttggtcgaa aattttat
//
LOCUS       DACEOE010000377          734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.411, whole genome shotgun sequence.
ACCESSION   DACEOE010000377 DACEOE010000000
VERSION     DACEOE010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.411"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(52..240)
                     /locus_tag="IW774_002898"
                     /pseudo
     CDS             complement(52..240)
                     /locus_tag="IW774_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            220..>734
                     /locus_tag="IW774_002899"
     CDS             220..>734
                     /locus_tag="IW774_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1155203.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT          255 a          114 c          162 g          203 t
ORIGIN      
        1 ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt tctaaaagtt
       61 cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt ttgtgagtat
      121 ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa gaacgcatag
      181 gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat taacagagta
      241 acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga aattgcacag
      301 cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga agaagccgga
      361 ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt cctacttccg
      421 ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa atttttaaat
      481 tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa aatcaaatct
      541 attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat taacgtgtac
      601 agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat tcagactgca
      661 atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca agaaccggaa
      721 agcagaatga taga
//
LOCUS       DACEOE010000378          732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.412, whole genome shotgun sequence.
ACCESSION   DACEOE010000378 DACEOE010000000
VERSION     DACEOE010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.412"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..296
                     /locus_tag="IW774_002900"
     CDS             <1..296
                     /locus_tag="IW774_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1155204.1"
                     /translation="AETGDAIAKEEVESFYTYLTKGLFSIQFSFDPEVIVLGGGVSAK
                     EGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRNDANLVGAAANYLAVRQGEN"
     gene            413..646
                     /locus_tag="IW774_002901"
     CDS             413..646
                     /locus_tag="IW774_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1155205.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT          287 a           85 c          164 g          196 t
ORIGIN      
        1 tagcagaaac tggagatgca atagcaaaag aagaagttga gagtttttat acgtatctta
       61 caaagggatt attcagtatt caattttcgt ttgatccaga agtgatcgta ttaggcggcg
      121 gggtttctgc aaaagaaggg ctcattgatg agatcaaaag ccgaatgaag aagctgacag
      181 aacaattcga tctgcatgat ttcgaaccgc agattttatt atgtgaatac agaaacgatg
      241 ctaatcttgt aggtgcagca gcaaactatc tcgctgtacg tcaaggggag aattaagagc
      301 tttagagaag gtgcagcttg cataaaagaa gtgaatgaaa tgaataggag atttttacct
      361 aaggaatgtt ttatttgctg cagtatgata aaatatctga gaggtgaaaa tgatgtcaaa
      421 aaaaaataaa gatattgaag tgcgtataga agaagtgaaa aaagatatca aaggtaagaa
      481 ttatgaagtc actcagttat ttatagggaa aaaaatgatt ggtgagatct tagcctatgg
      541 accaaaagaa tatgaaattt tctttggtga agaagatttt ggaaaagaaa aaagtcttga
      601 aaatgcgata gaaacagtga ttcgacactg gaatctgcac gaataaaaaa atatcaaaaa
      661 aactttcaat aaagggttgc aaaaatctgg aaaattaggt attataacta agtcaggttg
      721 atacaaacct tt
//
LOCUS       DACEOE010000379          731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.413, whole genome shotgun sequence.
ACCESSION   DACEOE010000379 DACEOE010000000
VERSION     DACEOE010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.413"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            55..731
                     /locus_tag="IW774_002902"
                     /pseudo
     CDS             55..731
                     /locus_tag="IW774_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          297 a          125 c          137 g          172 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt c
//
LOCUS       DACEOE010000380          730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.414, whole genome shotgun sequence.
ACCESSION   DACEOE010000380 DACEOE010000000
VERSION     DACEOE010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.414"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            51..704
                     /locus_tag="IW774_002903"
     CDS             51..704
                     /locus_tag="IW774_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1155206.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          307 a           61 c          134 g          228 t
ORIGIN      
        1 ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat atgaaaatat
       61 taactgttga agatgataat atgataagag agggaataag tgaatatctt tcagaatttg
      121 gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt aatagcgata
      181 taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa gtgttgaaag
      241 aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt gatgaagaat
      301 ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca ttttcattgc
      361 cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat ttagagaagt
      421 ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata aatggtgaag
      481 aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat aataatggac
      541 aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa gaaactcctt
      601 atgatcgagt aatagatgta tatataaaag aacttagaaa aaaattacaa ttagactgta
      661 taactactat aagaaatgta ggatataaat tggagagaaa atgaaaaaat taaagatatt
      721 tccaaaaatg
//
LOCUS       DACEOE010000381          714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.417, whole genome shotgun sequence.
ACCESSION   DACEOE010000381 DACEOE010000000
VERSION     DACEOE010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.417"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..108
                     /locus_tag="IW774_002904"
     CDS             <1..108
                     /locus_tag="IW774_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1155207.1"
                     /translation="FTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
     gene            212..358
                     /locus_tag="IW774_002905"
     CDS             212..358
                     /locus_tag="IW774_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ1155208.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLY
                     YLSN"
     gene            complement(355..>714)
                     /locus_tag="IW774_002906"
     CDS             complement(355..>714)
                     /locus_tag="IW774_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1155209.1"
                     /translation="VDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSISNTNDIDI
                     YFADPGTPSQRGLNEHSNGLLRKDGLPKEMEFNQVNQGFISSVASKRNHIPRKSLNYQ
                     TPLEVFLSYVNGKFCLA"
BASE COUNT          249 a           89 c          134 g          242 t
ORIGIN      
        1 ttcactgaat atcaagagga attaataaga aatttgagtg aaattggaag aactgctgga
       61 ataaatggtt tatatcgagc agattatgga gataaatctc aattttaatg atacttataa
      121 tattaaatat gtctcaaaat tttttgatta gaaaaatagg tatattgaat cagtgacata
      181 aaagtattta tagtttgtag ggggaatcgt aatggaagaa aaaaatagac taaatgctaa
      241 acaatgttca gatcaagagc ttaaaaaaat taaaggaggt gctggaacaa aacctcaagg
      301 aaaaccggca tcaaatttag tggaatgtgt tttttcatta tattatttgt caaattaagc
      361 gagacaaaac tttccattta cgtaactcaa aaaaacttct aatggtgttt ggtaatttag
      421 tgattttcta gggatatggt ttcttttgga cgcaacggat gagatgaatc cttgattgac
      481 ttggttgaat tccatttctt ttggtagtcc atcttttcgg aggagtccgt ttgaatgttc
      541 atttaatccc cgttgggaag gcgttcctgg gtctgcgaaa taaatatcaa tatcattcgt
      601 attactaata cttttccaat tagagaattc ttttccacaa tcaaaggtaa ttgatttgaa
      661 aagatttttg ggtacggatt gaagccattc attaatcgaa ttttcaatat caac
//
LOCUS       DACEOE010000382          698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.419, whole genome shotgun sequence.
ACCESSION   DACEOE010000382 DACEOE010000000
VERSION     DACEOE010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.419"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            45..>698
                     /locus_tag="IW774_002907"
     CDS             45..>698
                     /locus_tag="IW774_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1155210.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKILEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKINNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     "
BASE COUNT          281 a          108 c          113 g          196 t
ORIGIN      
        1 agaattaaaa tatcagctaa aataaaagtt aaggagcatt gagtatgaca attaaaacta
       61 gtctccaaaa tcaagcttac caatctattc gccgacaaat tatctatgct gacttagaac
      121 ctggaaaaaa agtatcagaa aaaatattag aagaaacact ttctattggt cgtacaccaa
      181 tacgtgaagc actgattcaa ctccgacaac aaggtcttgt tgatacaatt cctcaaagcg
      241 gaaattatat ctcattaatt gatttagatt cggccaaaaa tgctcgattt gtcagagaac
      301 atttggaacg acaaattatg ttagagtgtt gtgccaaaat taataaccaa aaaaaacgga
      361 ttttagaaac tataatttca gaacaagaaa aagctgttag tcaaaaaaat gctcgtagtt
      421 tttttcataa tgataattta ttccataaag cttgttttga aattgcagag cgagaagaag
      481 tatggcaatg gttagaaaat aacaatacac acttagaacg atttcgttgg ctacgagtaa
      541 aaacggaagg attaaagtgg gaaaaaatta tggaacaaca ttatcaacta tttgaggcat
      601 taacaaaaca agatccagaa gaagctaact ttctaacatc gttgcatctt catttaatgc
      661 ttaatgagca aaatattgtt ttagctactc atccaaaa
//
LOCUS       DACEOE010000383          676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.422, whole genome shotgun sequence.
ACCESSION   DACEOE010000383 DACEOE010000000
VERSION     DACEOE010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.422"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..676
                     /locus_tag="IW774_002908"
                     /pseudo
     CDS             6..676
                     /locus_tag="IW774_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          244 a          122 c          142 g          168 t
ORIGIN      
        1 tttttatggc taagtatact tttgaactta aattaaagat cgttcatgat tacttggatg
       61 gtaaaggcgg aagtgattat ctggcaaaga aatattccat taaggctcca tcacaagtga
      121 agcgctggat caatgcctat caagaatttg gtgaagaggg tctagtaaga aagcgacaaa
      181 agaaaaaata ttctgttcaa tttaagctgg atgcgataga gttatatcta acaagtgagc
      241 tgtcctatcg tgaagttgcc aacacactta acatgaacaa ccctaatttg atcgctagtt
      301 ggatgcgtca attccgtgag ggtgggatcg atggactctc aaaaacgaaa ggatgtccac
      361 ctatcttgtc taagaaaaat gaacccaaaa ataagaaaaa atctacaact aaagcaacgt
      421 ctaaagaacg tgaacgcatc gaggagttgg agaaacgagt ccgttccctt caaattgaaa
      481 atgccttttt aaaagaattg aggaagctgc gaaaacagga agcccaacaa cgacgaatga
      541 agcaatcgca cgaatcatcg caagcctccg aggaccgttt aaattagttg agcttctaga
      601 aactttaaag ttccctaagg caatctttat gtactggcaa aaacgattgg accgtaaaaa
      661 tccagatcaa gagatt
//
LOCUS       DACEOE010000384          668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.423, whole genome shotgun sequence.
ACCESSION   DACEOE010000384 DACEOE010000000
VERSION     DACEOE010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.423"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..610
                     /locus_tag="IW774_002909"
     CDS             179..610
                     /locus_tag="IW774_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155211.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          260 a          100 c          135 g          173 t
ORIGIN      
        1 gaaggctatc ggacaccaat tagttttgaa caactggttg ggtcaataga ccgttctact
       61 ttgaacagtg ctcaactatc tattggcgca gacggagcca ttaaatggag aagaaattca
      121 agttatggat cagcttattc atttgttatc acttattcaa tttaagggag gaaatctaat
      181 gaaagtagtt tacaaatcaa tcaagcctta cggattcgag caaatcattt tgaacaatca
      241 agaaaatatc cctgaaaact gtacagagat taaaccacca gttcctaact ggagaccaag
      301 atttgatttt gataaaaaac agtgggttga gttagctact gaagaagaga aaaaagggaa
      361 tgctgttgat agcgtagatg aattggctaa tattaaggca ttatacgaaa cgctgaaggc
      421 agaaaatgat gaattgaaac agctaaattc taaagcaatg ctaaacaatg tagcaatcaa
      481 gcaagaaaat gtcttattga aagaaaagtc agaaagttta gcgcagttga attcaaaaac
      541 gatgcttgct tctgtacaaa ataccaagga aatcgaagaa atcaaaaaac aattacaagg
      601 tgggaagtaa catgtattca tatgaagata tcaaactaat gtatgactgg ggctgtttca
      661 ctaacgaa
//
LOCUS       DACEOE010000385          636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.429, whole genome shotgun sequence.
ACCESSION   DACEOE010000385 DACEOE010000000
VERSION     DACEOE010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.429"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            150..380
                     /locus_tag="IW774_002910"
     CDS             150..380
                     /locus_tag="IW774_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155212.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTAERK"
BASE COUNT          270 a           95 c          101 g          170 t
ORIGIN      
        1 atactgaaaa caagattaat agctaccagc aagaaattga aacatcgaaa acgcaactga
       61 aagatcaaga agagatcatt caaaaacttc aagaaaaaat tgatcgtatg acaaaagaaa
      121 agatttagaa ggacaggtgg agtgaaaaaa tgaagcgaac aaacttggct gacaaagata
      181 ctttcgctga gcaaaaagta ttgattaaag cctatctcaa agttgtaaaa gctaaagaaa
      241 tatgtcgaga agtgaattta tctaacacaa caatatctga gttgaagaat ggtcgaaaga
      301 atttagatac tttaaaattc agatcgtttg aagctctata tgtagctgct aaaaaatatt
      361 ttacggctga aaggaaataa aaacaggagg attttatgtg aaaaaagtat atcaaaaatg
      421 tttatggatc agctatatca ccgtaacaca aacatcaagc acataaaaca tttaacactc
      481 ctcctttctg gctgaagatt tcaaccaaac aggaagacag cgtatcaatt atagcataca
      541 ctttttctta aattcatttt tcatatgaca aaatatttcc ttctaatcga caaagttaga
      601 aggatttttt attgtctaaa tacacaaata acctta
//
LOCUS       DACEOE010000386          611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.431, whole genome shotgun sequence.
ACCESSION   DACEOE010000386 DACEOE010000000
VERSION     DACEOE010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.431"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..611
                     /locus_tag="IW774_002911"
                     /pseudo
     CDS             1..611
                     /locus_tag="IW774_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          214 a          109 c          112 g          176 t
ORIGIN      
        1 actaaagaag tactaagtta tgcgattgct ccgactgaat cgacctacgt ttggaatgag
       61 ttgttacagg acatttactc cagaggggtt catgatgtct tactgtttat taccgatggc
      121 ttaaaaggca tgaaagatac gattcaccag atttatccta aagctaaata ccaacattgc
      181 tgtgttcacg tttcccgtaa tattgctcat aaagtacgtg tcaaagatcg aaaagaaatc
      241 tgtgatgatt ttaaggctgt ttatcaagct agctcaaagg aagaggcaaa taccttttta
      301 gggagtatga ttgagaagta gcaaaaaact tatcctaaag tgacgcagtc actgataaaa
      361 aatcaagatt tattgacttt ttatgagttt ccgcctggta tccgccgaag catttactca
      421 actaatttaa tcgaatcttt caagaaacaa atcaagaaat acagccgcag aaaagagcag
      481 tttcaaaatg aagaatcact agagcgtttc ctagtatcca tctttgatac atacaatcaa
      541 aaattcttaa atagaagcca taaaggcttc caacaagtga cggatacatt agcttcaatg
      601 tttactgagt a
//
LOCUS       DACEOE010000387          609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.433, whole genome shotgun sequence.
ACCESSION   DACEOE010000387 DACEOE010000000
VERSION     DACEOE010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.433"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..394)
                     /locus_tag="IW774_002912"
     CDS             complement(95..394)
                     /locus_tag="IW774_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1155213.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          193 a          113 c           70 g          233 t
ORIGIN      
        1 ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg agattgacta
       61 tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa gtaatcggtg
      121 ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat atctccatat
      181 aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt aaactacgtt
      241 ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc tttgcgtcta
      301 tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca tataatttta
      361 gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa aaatatattt
      421 cttcccacta cattctataa acaaagtata cgctacatct agattttttt aaatcattac
      481 tggattgtta aaacctttca atacactgtt atggcaacga tttttatttt aaaaacattc
      541 taaattaaat cagtaaaaat ttttattcta agatttttat tagaattacg cctaatttgt
      601 gtctctcag
//
LOCUS       DACEOE010000388          584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.435, whole genome shotgun sequence.
ACCESSION   DACEOE010000388 DACEOE010000000
VERSION     DACEOE010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.435"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(11..>584)
                     /locus_tag="IW774_002913"
     CDS             complement(11..>584)
                     /locus_tag="IW774_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1155214.1"
                     /translation="EFYYKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPP
                     KIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFN
                     SSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEK
                     LFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          188 a           84 c           71 g          241 t
ORIGIN      
        1 aggtactatt ctattcatct atatttccaa ctttcaaaat ttctttatca attctattta
       61 atagtttatc agagtaatcc gatttaactt ttcttacaaa taatttttca ctttctttta
      121 tttgatctat atccctttca tcaaaccatt tgttcatgga aggatcaata atatgaatat
      181 ttgctaattt cgaagtttgt aaagtagttt cttcaatatt tttcaattca agtggtaaat
      241 ctggtcctcc aataacattt ttatttttga acgaggaatt aaaaaaaagt gtatgaaaaa
      301 atttttcatc tggggcaaac atacctattt taaaattcag gtagtcttgt tgattttgtt
      361 catacatttg tattaattct gttaggcatt ctttcgacaa agcccaccat tgagaaccat
      421 gatacaaatc aaatttatgg tgtctaattt ttgggggaag tttaatcatc ctcagacaac
      481 gattaattat agctctaaaa attttaaatg cagttgtgtt attcctatta attctaggat
      541 aatcatgttt ttgatataca tctatatgtt tataataaaa ttct
//
LOCUS       DACEOE010000389          548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.441, whole genome shotgun sequence.
ACCESSION   DACEOE010000389 DACEOE010000000
VERSION     DACEOE010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.441"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..548
                     /locus_tag="IW774_002914"
                     /pseudo
     CDS             1..548
                     /locus_tag="IW774_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
BASE COUNT          201 a          112 c          115 g          120 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaaaat tctaaatatt
      241 tctcgtcaga cctactatta tcaagcgaaa ccgatcgaaa atgagtccga cttagaagag
      301 atcgttcagg aagagtttat tcgaaaccga aaggcttacg gtacccgaaa attgaagaag
      361 tgtttagcaa agcgtgggct tcaactcagt cggcgccgaa tcggtcgaat catgaaacgc
      421 cgcggattga catctaccta tacgatcgct cattttaaag ggcaacgaac agcttgtaat
      481 gaagcgaaaa cagcgaatgt attagatcgg acctttacac aagaacagcc attggaagcc
      541 atcgttac
//
LOCUS       DACEOE010000390          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.450, whole genome shotgun sequence.
ACCESSION   DACEOE010000390 DACEOE010000000
VERSION     DACEOE010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.450"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..132)
                     /locus_tag="IW774_002915"
     CDS             complement(<1..132)
                     /locus_tag="IW774_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1155215.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACEOE010000391          493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.denovo.456, whole genome shotgun sequence.
ACCESSION   DACEOE010000391 DACEOE010000000
VERSION     DACEOE010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.denovo.456"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(33..118)
                     /locus_tag="IW774_002916"
     tRNA            complement(33..118)
                     /locus_tag="IW774_002916"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(82..84),aa:Leu,seq:taa)
     gene            complement(135..206)
                     /locus_tag="IW774_002917"
     tRNA            complement(135..206)
                     /locus_tag="IW774_002917"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(172..174),aa:Gly,seq:gcc)
     gene            complement(218..290)
                     /locus_tag="IW774_002918"
     tRNA            complement(218..290)
                     /locus_tag="IW774_002918"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(255..257),aa:Thr,seq:tgt)
     gene            complement(308..389)
                     /locus_tag="IW774_002919"
     tRNA            complement(308..389)
                     /locus_tag="IW774_002919"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(353..355),aa:Leu,seq:tag)
     gene            complement(397..469)
                     /locus_tag="IW774_002920"
     tRNA            complement(397..469)
                     /locus_tag="IW774_002920"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Lys,seq:ttt)
BASE COUNT          127 a          137 c          122 g          107 t
ORIGIN      
        1 tatccagttg agctatgggc gcatatttga aatgccgagg accggaatcg aaccggtacg
       61 gtgatcactc accgcaggat tttaagtcct gtgcgtctgc cagttccgcc accccggcgc
      121 gattgctttg gcaaagcgga aaacggggtt cgaacccgcg acccccacct tggcaaggtg
      181 gtgctctacc actgagctat ttccgcgtgt ttaaagatgc cggctaaagg acttgaaccc
      241 tcgaccctct gattacaaat cagatgctct accaactgag ctaagccggc atgtaaaact
      301 taaattatgc gggtgaaggg acttgaaccc ccacgccgta aggcgctaga tcctaaatct
      361 agtgcgtctg ccaattccgc cacacccgca aaaatatgag ccgtacaggg ctcgaacctg
      421 tgaccctctg attaaaagtc agatgctcta ccaactgagc taacggctca tatggaggtt
      481 aacgggatcg aac
//
LOCUS       DACEOE010000392        26953 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.guided.018, whole genome shotgun sequence.
ACCESSION   DACEOE010000392 DACEOE010000000
VERSION     DACEOE010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.guided.018"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            368..931
                     /locus_tag="IW774_002921"
     CDS             368..931
                     /locus_tag="IW774_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155216.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGEFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
     gene            918..1802
                     /locus_tag="IW774_002922"
     CDS             918..1802
                     /locus_tag="IW774_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAQ1155217.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            2196..2693
                     /gene="dfrG"
                     /locus_tag="IW774_002923"
     CDS             2196..2693
                     /gene="dfrG"
                     /locus_tag="IW774_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1155218.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2765..4717)
                     /locus_tag="IW774_002924"
     CDS             complement(2765..4717)
                     /locus_tag="IW774_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1155219.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4724..5020)
                     /locus_tag="IW774_002925"
     CDS             complement(4724..5020)
                     /locus_tag="IW774_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155220.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            5586..5840
                     /locus_tag="IW774_002926"
     CDS             5586..5840
                     /locus_tag="IW774_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155221.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            6237..7838
                     /locus_tag="IW774_002927"
     CDS             6237..7838
                     /locus_tag="IW774_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1155222.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            8072..8371
                     /locus_tag="IW774_002928"
     CDS             8072..8371
                     /locus_tag="IW774_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1155223.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            8505..8882
                     /locus_tag="IW774_002929"
     CDS             8505..8882
                     /locus_tag="IW774_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ1155224.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLIC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            8931..11294
                     /locus_tag="IW774_002930"
     CDS             8931..11294
                     /locus_tag="IW774_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1155225.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGSYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
     gene            11612..12211
                     /locus_tag="IW774_002931"
     CDS             11612..12211
                     /locus_tag="IW774_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155226.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            12323..12547
                     /locus_tag="IW774_002932"
     CDS             12323..12547
                     /locus_tag="IW774_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155227.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            12899..13135
                     /locus_tag="IW774_002933"
     CDS             12899..13135
                     /locus_tag="IW774_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155228.1"
                     /translation="MLSDYKEELKLIENRVLGFVECKDNLDDTLEVLESIIFNTKVLD
                     LKAINGDMTTHIILDEISAKEIGETLIAWANKEL"
     gene            13215..14069
                     /locus_tag="IW774_002934"
     CDS             13215..14069
                     /locus_tag="IW774_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1155229.1"
                     /translation="MMKSNFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            14082..14519
                     /locus_tag="IW774_002935"
     CDS             14082..14519
                     /locus_tag="IW774_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155230.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            14537..14836
                     /locus_tag="IW774_002936"
     CDS             14537..14836
                     /locus_tag="IW774_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155231.1"
                     /translation="MDHKSNYSYKENYIWKYVVAVFLLVGGIIALVKWAIIPNALVFI
                     NWFVPNFWSFFGYILIIWFMFKVLSFFWSPVYSLLGKWSYLILIATAIYIIMQIN"
     gene            14839..15282
                     /locus_tag="IW774_002937"
     CDS             14839..15282
                     /locus_tag="IW774_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1155232.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLSHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSVVFYT"
     gene            15427..15750
                     /locus_tag="IW774_002938"
     CDS             15427..15750
                     /locus_tag="IW774_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155233.1"
                     /translation="MARSRIAKIEQTKKDKKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKNKG
                     IMIPA"
     gene            16168..18978
                     /locus_tag="IW774_002939"
                     /pseudo
     CDS             16168..18978
                     /locus_tag="IW774_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19074..19829)
                     /locus_tag="IW774_002940"
     CDS             complement(19074..19829)
                     /locus_tag="IW774_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1155234.1"
                     /translation="MSNYYHLLNKLEDLNLKHTREILPEYLDTIVQENLSFVEALNHL
                     MTEEINSRNERQKENRLRKANLPFKKTINDFDFGFQPKINRTEILDLCTLRFMDTNNN
                     ILFIGNSGVGKTHLAIAITLEAITKGYSCHFALSNELVNRLNRANQRGNLDRLLKKYA
                     SYDLLIIDEIGYLPFTPDGAKLFFQLINLRYEKKSTIITTNIPLSQWAETFQDKKLTN
                     ALIDRLVHHAKIIQITGKSYRIKDYKEKKLSYK"
     gene            complement(19844..21070)
                     /locus_tag="IW774_002941"
     CDS             complement(19844..21070)
                     /locus_tag="IW774_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1155235.1"
                     /translation="MRKDIYEGVLPFIMTHTKPNYAALAKQYNCDYRTVKRYYEAGIS
                     NKLPLLAQKNKNKKTLVSDFEDIIADKLALGCSSSAIYHFISKRGYRGSARTLRRYCR
                     NIKADKIQKATIRVETTPGLSAQVDWKEDLKLVNKFGEVISCNIFLYVLGYSRIKYLE
                     LTFDRSQTTLFRCLTNAFQYTGGVPTEIWFDNMKTVVDRSRSQFSKVIFNERFRQYAK
                     EAGFLPIACRAFRPQTKGKVEALARTIERIRVFNYEFESTHELEKIIQTIMDSLNTEE
                     ISQAIHECPSDRWSYEKEQLSSFDANQLKIYASVEKQIKRKVSNEAMVQWNQNKYSVP
                     VNYIGKILTLRVKEDKLYIYDDSTLIRTHTMTHQLLNYHRNDYIEILRSDVFKHLEDE
                     ELERFVDENLQAYDEL"
     gene            21178..22005
                     /locus_tag="IW774_002942"
                     /pseudo
     CDS             21178..22005
                     /locus_tag="IW774_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22065..22427
                     /locus_tag="IW774_002943"
     CDS             22065..22427
                     /locus_tag="IW774_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155236.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSIVFTDMVHKMLNGEPINFNFDYMLEKAQSNQIVAFIVSIYYFIEKSIFSLKDF
                     FFEMLTKLTTFYNKISKN"
     gene            22661..23083
                     /locus_tag="IW774_002944"
     CDS             22661..23083
                     /locus_tag="IW774_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155237.1"
                     /translation="MIYVAFFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETAD
                     YKFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            23095..24510
                     /gene="cpaF"
                     /locus_tag="IW774_002945"
     CDS             23095..24510
                     /gene="cpaF"
                     /locus_tag="IW774_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1155238.1"
                     /translation="MRVVEEKKISPKIEETPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSASEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            24527..25342
                     /locus_tag="IW774_002946"
     CDS             24527..25342
                     /locus_tag="IW774_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155239.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYLNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            25342..26139
                     /locus_tag="IW774_002947"
     CDS             25342..26139
                     /locus_tag="IW774_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155240.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKKDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIRPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            26157..26519
                     /locus_tag="IW774_002948"
     CDS             26157..26519
                     /locus_tag="IW774_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HAQ1155241.1"
                     /translation="MQEQSVMEIIKWFIGLMMIMAMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDRRFTIESDKLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVTLSFNGNSVSQIR"
     gene            26691..26912
                     /locus_tag="IW774_002949"
     CDS             26691..26912
                     /locus_tag="IW774_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155242.1"
                     /translation="MEAIQENRKDNQETSEKNKKKRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
BASE COUNT         9914 a         3516 c         4875 g         8648 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact tagaattata atcacaaact
       61 taatagtaat tactattggg attacaacat taacaaataa agctttaata tcaagtatga
      121 ttttaatagt gtttgatttt attatcattt atagtttgct gtttttaata aagaaagtaa
      181 ctgaatattt aaatccgtta ggcacgaaat aaattttaat tgaggttgaa ttatagcggt
      241 aaatcataca aaatagaaat gtagtcttgt taagcgtctt gactatctaa ttttgaatga
      301 ggagggttgt atatagaaaa agggtagtta aattcgtaga tcaaaaggct acgaaggaga
      361 ataaagaatg acagctattt ataaagcact aaaaaattct aacgtatcag agtacgttaa
      421 gtcgagagta ttaaataata atttagatta cattttagaa aatggtgttg ctgatttaat
      481 cagtaaatta agtgatccta tagatataga tcatttggtg gtatggcaag atggtacttt
      541 agaagtatca atatatggag aattttatag tagaaataaa gtaactacat tcaaaaatag
      601 tattagtggt cgagaagatt taaaaggaat caataaaaat agtaaattct atagaacaaa
      661 taaagaattg ctagaaaaaa tttcaaagcg tgtttttgaa ttggtgaatc cattttcaaa
      721 aaaacgtagt aaagaacttt ttggaatttt ttatgatggt tttagtgcaa aaagtaatgt
      781 agaaaaattt ttttatttta atgattcagc ttatattgcg gttaaagaca gtaaaagaat
      841 ccctgaagca actttagaat atttagagcc aattaaattg tctgtttttt attcacttta
      901 tgagaaagaa atcgctgatg aagcggttta aggggagtaa aatgagtaaa aaaataggca
      961 aagagtcatt atcgctcgga ttgaaaaaag aaattgttaa atttaaaagt atgtatagag
     1021 agccggaatt tagactcatt tttcaattag atatagtgag agggctgaaa attagtttta
     1081 gttgtttagg ttatctggtc tggctattca ttgctagagt aggatggagc aatgcttata
     1141 gcttttataa actgttcgcc ggaactgatc gattaaaaga atcattagag gtgaaagaag
     1201 ccttaaatat tttaggaagt cttgctttca gcctgtttat ttttgcttta tttatcttac
     1261 aagtaggaaa aataatgatg tcaacaaatg acaattatat taattatttg aagccttttc
     1321 aaaaaggaat aaaaatcatt tgtagacagg gcacagtttt atttctactt agcctgtatg
     1381 agcatgaaat tcattttcaa aattattata atacgacaac taaacttggt gcatttttac
     1441 tcctatattt agttagtaat atagctgaaa aaatgctgtt taaacatatt gtaaagcaag
     1501 tcttttctga tattcaaaag gaaagctatt gtttaaaaac accttatgga aatttaaaaa
     1561 atgttgagct gtttttattt aaaatggata gtaagtggga taaatatatc tatagaagta
     1621 aagaagtatt tttaactggt agttataaca aaactcttca ggagagcctg tacgacacag
     1681 gagcaatatt taataatgaa cctatggttt tgaaagattt ctttggagat tatgaactag
     1741 tttggaatcc aaaacagctt agtgaacagg gggcagaaaa tgaatttgct gaagttcact
     1801 aacatagtgt acagaaaaac attatctgaa agaagaacgc cttcaaaaga aagtacaaga
     1861 acgattgggc cgtacagaag actaacgaaa gatcgtaaaa aaaaggaaag acaatgaaaa
     1921 ttaagtagtt cagttttgga gtacaaaatg acaaaatgta cttcaaaatg gactactttt
     1981 ttcattttaa cttacatacc aaattacata caaaatgatt tataaaatgc aattgaaaat
     2041 gataattgaa tgaaaatttg tgattttgtg gtattatgat aatataactt atagaaaaaa
     2101 ttgcttattc aactaacggg caggataatg agaaaaggtt ttttgataga aataagaaat
     2161 tagatggtaa attagttaaa aaggctgtgt tataaatgaa agtttctttg attgctgcga
     2221 tggataagaa tagagtgatt ggcaaagaga atgacattcc ttggaggatt cccaaggact
     2281 gggaatatgt taaaaatact acaaagggac atccgataat attaggtagg aagaaccttg
     2341 aatcaatcgg aagagcctta cctgacagaa gaaatattat tctgacgaga gataaggggt
     2401 ttacctttaa tggttgtgaa attgttcatt caatagaaga tgtttttgag ttatgtaaaa
     2461 acgaagaaga aatttttatt ttcggaggag aacagattta taatttgttt ttcccttatg
     2521 ttgagaaaat gtacatcaca aaaatacatc atgaattcga aggagatact ttttttccag
     2581 aagtgaatta tgaggaatgg aatgaggtat ttgcccaaaa agggataaag aatgataaaa
     2641 atccgtataa ctactatttt catgtatatg aaagaaaaaa cttattgagt taacgggtgc
     2701 gttaattgaa gaagaacagt aaagataaag cacaagagct tgggattact ccaagctctt
     2761 tttgtcattt gaaataaatt atatctttcc aatcaaattg tttatctgta atccctaatc
     2821 tttgagcagg tgtcaaaacc tttttatccg ctgatttaaa agggcgacaa aagttgtagt
     2881 aagttcttag aattgtaatc gcaaattgag catacttcgg attgaagtta gcgtaaatat
     2941 agctcttctt atcaccacga gctgttgtta atggtctttc aagagatgat atataacgtc
     3001 ttatctgttg aataaagcta ttggtcgaat ggtcattaac atttaatagc atctttgcga
     3061 tttcgtttgg ttccagagca gataaatccg ttctacaatc aactgaataa aatcctttat
     3121 ccttagtggc taatggatgt ttgatagggt tatcggcata tttgcgatat ctttctccat
     3181 ctttactaat agcctcttcg tggaattgat gacttgttag taattcagtt agataatgag
     3241 atgccaaagt ccgtagaaac tttgttttga atcctctgat atctccccaa tcgagcagtt
     3301 ctgcttttgc ttgtccgaat tcttttaagc attgttttct gcttttcgtc ttgttaactt
     3361 ggcaaataaa atgatgtgca tcgctcaacc ttaattcttt cgtgaatact ctataaaagg
     3421 cgtttctaat agagaagtca tcatcactaa tcattcgcca ttcgtcggca ttaaccattt
     3481 gtttaattaa ccaatagtga gcagttgtag tataaggagc atcaacattt aaaccatcga
     3541 caaatctact cctattagta attttcccta attcgtgaag ataagcattc ctattttcgc
     3601 tatcgttagc ggatggttct tgtggataat aagaccaatc taacctatca tttttacgac
     3661 taaaagtatt gagatggtct tctttgaatt ttcttgtatc ttcatttaat tcatccattg
     3721 aaatattcca atcataggca acatcagaac ggaacacata tcttgataag acctcagcag
     3781 atacaactat atacgtttgc atattaaggt cttcaaaccc cgtatatttc tttgaacctt
     3841 gtcctttctt acggacgtta tttaggtagt agtgcatctt gtccgtatta agccacatct
     3901 cgttaaattt ctttgtttga aggggttgtg tctcatatcg ttctaaaaac tccaaacaac
     3961 gcctatacag ccactccagc ttatgatagt aggttccaac tccaattcct aaaatatcac
     4021 aagtacgact gactgaaacc cttccaacaa ccatcttagc gaacattgga agaatggtgt
     4081 tcttttgttg gtgataagta gtggtttctt ctctttttgg tagaacatta gtgaacttct
     4141 tacacgcctt acattgatag cgttgagatt tgcccctact tttccccctt ttgtaaaatt
     4201 gcttcggctc attgaatggg gttgattctt cttccgagca gccttcttta tgaaattgat
     4261 aatctggctc aacatcttgg atgctattga tttctataag gcgtttaatt tcttcaacaa
     4321 cagaccaatt agataaaggg gttactgtac agttttgcga tacacctctg ccaataggat
     4381 tgtcattgca gtacatacct ttatgacctt tatctttgga actaccagtc attttatatc
     4441 gacttggttt cccttttaca tctttgaatt tgtgctgttc tttcccaaaa ttacaacaaa
     4501 acggattgat gcagtaatta tattgaatgg gatattcaga accgttccat ttaaaagacg
     4561 ttggcaagaa caacattttg ttataccttg ttgcaaatcg ttttggagtt aataaatcat
     4621 attgtttagc cctatctttt aattcttcgt ttgaaatcgg aattttaaca tctacaattt
     4681 catcttcctt cgtggctaat cttttaagct tggtcattgt ctatcaaccc actctatctt
     4741 tgatacccat tttttcaaca atccgcttat tacttcgctt gttggcaatc cattcaataa
     4801 acttcttatc gtctaaaatg ttcaataaga attcatccac tgcttcgctt tcagtacgct
     4861 ctgcatattc agcgtattgc ttaactatca cccttacttg ctctgatatt tcccaatcca
     4921 cagacttagc gttcttattt tttggctcaa gaaattgcat aggaaaatgc accccttttt
     4981 gcttagtaaa ttgtaaccat tttaacacac caatttgcat tcctacaata attatttcac
     5041 gttcgcattt ttatatacat tttaacttac attttgtggt gtaaattaac aacaaacgtt
     5101 gatttaacaa taaaaaacga gtatcaaatt cagttgcaat ttgatactca tttaggttta
     5161 cattttcact ttttgtactc caaaactgaa ctacttaaat gaaaatgtct ttcctttttt
     5221 tttgactaca agtagcctga aaatgaaatt tcaaaagact acaagtagtc ttttgtggca
     5281 aaaataaaga gccaacgaaa gctaccaaca atcgttgact cgtaagactg ttcgtagtaa
     5341 gaaaaatctt tggcaatcca cgaagtcttt atttcataat ttatataaat gattatacag
     5401 tcactttcct gaatttgcaa gtaaaaaagc taagaaatat tgtttaagcg tttccttaaa
     5461 agaatcttta aataaatttt tttccaattg aaaacatgac tacaagtaga ctatttcttt
     5521 tattccaagt tgactacaag tagtcaaggt gatataatat atatatcaag aaaggagaaa
     5581 caaaaatgta taacgtagga gatcaaatag atgagatcgt aaaaataggt aatgttaaac
     5641 ttgttaaaag taagtactat atttcagaaa ctgaaataat ttttaatact gaatcaggat
     5701 gtattctaat ttggaaactg ccttttccca aagacgaaac aacactgaaa aaaataaata
     5761 ctttaaaaat cagatattgc attcatgaaa ttattgataa aaatacttat gttataaatc
     5821 aagtaaaagt gatggggtaa aaatctttta aaaaaactct tgttaaaagt aattctatta
     5881 gcttataatg ttggtattag ttctgaagcc aaaggaacac taaaaaattc tagtagaaaa
     5941 gttagttaca gactctctac tatgtatgaa tgtaacgaaa aaaatagcga aaaagataat
     6001 taaataagac taccaatctt atttaatgga acttctgtag taagaaaagc gatttggcaa
     6061 tccacgaagt ccaccaagat gagctaccaa ctcattttaa tttttgcacg tttaaagttt
     6121 agcatttaag tgcttactct aaagacatag tgtaatttta ggactcctta ttacggaatc
     6181 taaagttacc ctatgtcttt tttgctattc aaaattgata gcaaggagaa agtgcaatgt
     6241 tgaatagttg cgagtatgcg gacgttttag ctaaaattct taaaaatgga attaaacagt
     6301 ttaatagcaa aaatctagtt gcttttaaaa agaatagcaa taagattggt gctattgccg
     6361 gatttacttc taaaacaaac atgataaaga ctaaaggtct tatatatcgt tctattgatc
     6421 gtgtcttgga agattcagat gtattaacac actggactcc taacgtgtat tcatggcttg
     6481 gtggcggtga aaacaaaaca atttttggtc acaaagaagc taacttaatt cagattaata
     6541 cttttgtagc agatattgac tatccagtag gagaaaagaa aacggatttg aatttactag
     6601 ttctttatct attggaagaa gggttattgc ctacactgat tgtagatacg ccaaaaggtt
     6661 atcattgcta ttttctgatt caaaactata accaagaaac tgattcattt gataaggcaa
     6721 gctatatttc taattcaaat gattttaaat ctttgagagt tgctaaaaga atatcagaaa
     6781 atatcagaaa atctatccaa aatcgtttac cacaagtcga tatgggctgt aatcattttg
     6841 gtatttttag atttccaact aaaaaaaata ttgtacatta tgaacctaat ttcgtagata
     6901 cgtttgaagg gtatttgaaa tggtcaaaag actttgaaga aaaagaatat gctttaaaga
     6961 ggtctaactt gtcattatta gctcctaatt ctactggcaa gaaagggtct aaacgacaaa
     7021 ttgatactaa atggtacgac tttttaattc atacggatat tgctgaagga gatagaaata
     7081 aaactatttt taccttagcc ctggcatgta aatcaagtgg cttatctatt ggcgagtgta
     7141 tggatcaatt ggatcaactt caattcaata atgatttgtc ctattctgaa gttgagagaa
     7201 caataagaag tgcttatcgt ggcgattaca aaggtgctaa aagttcatat gtcaatgaaa
     7261 ttatcgaaaa ttatgctaca cctaccgagt ttgaaaaata ctcaataaat gtaaatagac
     7321 aagctgttaa acgtaaaggg aatcgtgtcg atccgtctaa ttgggtgaaa tttgctaaac
     7381 ctagagaaga acgaaaatat agtcattttg aagaaagtaa agctgattta ttagcttact
     7441 tagaaagaaa acaaagatca ttgaaaaaaa atgagttgtt tttaagtgtc tctatgggaa
     7501 ctatatctga agaaacacaa attcctgtat ctacgttaaa ggtgatttta aaagagctta
     7561 gaacggcgaa tacagtgata ttacgaacgt ctcgtggtcg gtatggatcg actaaaatag
     7621 caacaagcaa atttgtggaa gctaaaatat taaatacagt tattcagaat aaaaaagata
     7681 aacaagtatt tgtcttagaa ttattaggaa cagcaaatag ccacgtagaa acgattttaa
     7741 gacgttatga agacaaaaca gtaccgttcc atgcgaacat actgaaagaa agagaaaaga
     7801 gcaaacatgc gccaattaga ggagctaata caggttagag ttctatttat ttttttgatt
     7861 tttaaatagc taaattgcca attaacatat ctagtgtttc tgcccactcc tattcctgtt
     7921 ttagcgtata ctaatgatta gtagttagta ctctctttta tgtatggagt gtatagaact
     7981 atttttactt gtgtcttttc ccttaatcca cgtgtacgga atacagctta taactgcgta
     8041 tgcagttatg tttgaaggag gaagattttc tttggtaatt aagaaatatg tttactcaca
     8101 cccgatcaat gtatttatta tcggtaagct aggtttatct gttgaggtgt tttgtgagtt
     8161 atatggattt aaacaaggaa ctctatcgtc ttggattact agagagcgtg aggtagctga
     8221 tttgcctatt tcttttttgt atgctttgtc tctagcttca ggagggaata tgaatgaggt
     8281 ttatgattgc ttgtgtgagt atgaaaaaga gtataaagag tatttgaaaa tttcagcccc
     8341 aaaacggaat aaaaaatata ttgaagatta gagaaagaag aaaagaataa tttgtttttt
     8401 gactacaagt agacagatag ctactctgtt ttttgatagt ttgatattgg ctgttttttt
     8461 gttagaatta atatatatca ggtaaataaa aaagggggct attaatgaat attgatggga
     8521 tttataatga atcagatttt aaaaaaggca tagtagatat tgatagaaaa gagtatttat
     8581 ttcctgatga aataggttta tttaaaattg taggtgaaac tgtctgtagg tattggggga
     8641 agaaaggaaa tataatttta cttgttgata cgattgaggg aagaaagtta aaattttcgt
     8701 tttggagtaa tacaaaatat tgttgtgaac ctgtacctaa aagggaagta gatgtggcta
     8761 aagaaagtgt acgattttta cctgatcgat caaaaataac tttaatttgt gaacggacaa
     8821 gtaatgctaa atttacaaga attagagata tactactttc gactacaagt agtcagtcat
     8881 agagtaagaa cttttggtta aaaatcaatt gaagaaagtg gaggaagtat atggcagtag
     8941 ttatattagc agaaaaacca gatcaaggag caaaatttgc ttcagcattg gccggaaaaa
     9001 aattatcgaa aaagagtggg aagtatgaat ttaaaagtga aatatttgga gatactattg
     9061 ttacttgggg aataggtcat ttggtaggat tatcattacc tgaaaagtat gagtggctac
     9121 cgaataaaga taaatggcat ttagataatt tgccttttct acctaaattc aatgaattaa
     9181 agtatgaagt ctctaaagga aaaaaccaac aattttcaat agtgaagtca tgtttagaaa
     9241 atgcagatga gataataatt gctactgatc cggatagaga aggagaaaat attgcttata
     9301 atattttcaa attatgcaag cagtcgattt ttaataaacc gataaaaaga ttatggatta
     9361 attctttggt tcaaaaagaa attatcaaag gatttaaaaa tctgcgaaat tctgaagaaa
     9421 cgatctctta cttcaaagaa gctaatgcta gacaaattgc tgactatcta gtagggatga
     9481 attatacaga gttgtattca ttaaagttgc aagaaaaagg tatatcgggt gtggtatcac
     9541 tcggtcgagt tcagacaccg gtaaacacac ttgtggttga aaacgatttg gcaattagaa
     9601 attttagacc ggaaaaatat aaaacgattg aatgtcatac aaccgataag caaccaaaag
     9661 caatatttaa gaatagtctt gaatacttta aattagaaaa atttgaagaa gacactaaaa
     9721 aattcggcct tgattccgcc agtactggaa tcataaaaaa aattgaggtt agtaaaaagc
     9781 aaacagaacc acaaaaattg tttagcttgg gtggtattca agaatacgct aataaaaaat
     9841 ggaaatattc tgctaaaaaa acagataaaa ttatccaaag tctatatgat cgtggatatt
     9901 tatcttatcc aagaaccgat agtgaattga taactacaaa tgaatttgag tatttaaaag
     9961 cgaatttaga cgtgtataag agtgttttag ggatcagtat tgatacacct tgtttagagc
    10021 ctaaaaagcg gtttgtagat aatgacaaag tattagagca ttatgcacta ataccaacaa
    10081 tggatattcc tgatatagca aatctgacat tagatgaaaa aaatatttat gagatcgtta
    10141 ctaggcgtgc gtgtctaatg tttgcagaac cttacaagta cgaaaataca aaagttacat
    10201 tagatgtaaa cggcttagaa tttaccacaa gtggtaatgt acctatatct ttaggttgga
    10261 agcaagtaga tactaattta gatgaatctg atgataaaga ggaaacatta cctaaatttc
    10321 atgaacaaga gacaataaat atagatatta gatttattga taaattaact aaggaaccta
    10381 aaaggttgac tgaaggtaaa ttattaggta aaaccggtat tatggcgaaa ttagggctag
    10441 gaacgcccgc tacacgttct aatattgtag acactcttct aaatagggaa tatattaaga
    10501 tagaaaacac gaatgtttat cctactccaa agggatattt attatgggat ttgacaaaga
    10561 ataaagacct attgattgga aaaccagaaa atacagcgat atgggaagaa tcacttggga
    10621 aaattgcgca aggtagctat acacaggaag cttttttaag aaatatctat aactatatca
    10681 ataagactgt ttcagatttg aaaaaagtag aatttacttc ggattattta gaaaagactg
    10741 taaatcaaaa taaatttgat ataggtagtt atcaagtgga agaaaagaaa aaagtttatt
    10801 ctattttaaa tattactagt ggtgaacagt tcgtgatatt taaaaatata tcaggaaaag
    10861 caatcaataa aaaagttctt caagaactat tactaaataa tcgtacagat aaagtccttt
    10921 ccggctttaa gtcaaaagaa ggaaaagctt ttaatgcaag attacttttc gatccaaatg
    10981 tgatgaaagt ggtaatggag tttgaaaaga acagtccaaa aggcgataaa aagagcaaac
    11041 acgatacagt aaatggttat gaaatagttg aaaaagcaaa agtatttgaa ataaaagaat
    11101 tagcaacagg tgaaagtttt attttctata aaaataatag tgggaaaact ttaagttcaa
    11161 aaacagtcaa agaattgtta gaaaatggga aaactaaaga gaaagtaacg ggttttataa
    11221 gtaaagacaa taaaaaatat agtgcttatc ttgtctttga taataaaaac aaagttataa
    11281 agaaagaatt ttaattctta gtggcggaaa tcatttgttt ttccgcttat ttttattcaa
    11341 aagtgcccaa aatataggta tactgaatta aggataaata caaaactagt tagaggacag
    11401 ttgaaaaaat acaaacaaat tagattgcta ttttgttaaa ttggaagtag aataataccg
    11461 ttttgatatg tatagtgtgt tttaccaata aaattacata aacgtgaata tatttttatg
    11521 aattaaatgt aaaatgagag caattagttt taaaaaagat tataggctgt taatcttagc
    11581 atatataatc cattggaaag ggggagatcc aatgaatcta aagaatacaa actcaaacaa
    11641 atctacacta tatgtaattt cagatgatta tataaataag ctgaaaaagg tggactatcg
    11701 tgtccaaaac aactatcgag aacaacgatt atattttaaa tcaaacttga aaatttctaa
    11761 caaaaatgaa gaaataaatt attatgtacc attggcttca gcaaaagaaa atcaacgttc
    11821 tttgactaat aaatcagtgt ttaagattta tggtgatcga gaagaaaagg actttttagg
    11881 agtacttcat ataaataata tgattcctgt gccaaaagat tgtgcaaaag tatggacacc
    11941 taatagccca aatgttgatg agaagtataa gatgttagta atcaaacagg caaaatattt
    12001 gaaattacat gagtctgaaa taaatgaatc atgcgaatta ctttataatt ctaagttagg
    12061 taaacttgat cctagttact ttaagaaaaa tcgtaaagaa gtaattactt atcaaaaaat
    12121 aatgacgaat gtagaatcat tggaagctgt tagtatagca gaatcccaaa gaaatattca
    12181 tgtacaaact caaagtatag atgtagagta agataggcta aacctatctt attttattat
    12241 tctattggat attatgatac attgaatttt aatcgaatat gtatttaaat gttattatac
    12301 tgtagaagat aaaacttaaa ggatgatttc tatggcgaga ccgagaaaat acaatacaga
    12361 agaagaaaga aaagaagcaa tgaaaaaggc taggaaaaaa tgggttgaaa aaaatcagga
    12421 aactattgcc tatataagag ccaaatcatt tgctaaaaaa ttcattgaaa tgtctgaaga
    12481 tgaagatatt gaattgttgg aaaaatggtt agaagatcgg aaaaataacg gtcaaaatga
    12541 tgaataataa ccattgacta caagtagtca atggtgtaga atggttttag ggatagaaat
    12601 atcccttttc taaaagtcta atgactacaa gtagtcgata gagggatcgg gaataaaaac
    12661 agacgattat ggcctaaaga aaggaggaat atgaatattt tgaggaacga tttattaaat
    12721 gagcgttgat aaatttcact aaatattttc ttaatatcta tgtattgttc aagcgtagaa
    12781 aaaaatattg aaattattac aaaaccgctc ataatggaaa ataacgaatt atattaatga
    12841 atcgttcact tttgaccttt tttactagat ttttttgtag gacttttaga gaattttttt
    12901 gttatctgat tataaggagg agctaaagtt gatagagaat agagtactgg ggttcgtaga
    12961 gtgtaaagat aatttagacg atactttaga agtccttgaa agtattatat ttaacaccaa
    13021 agttctggat ttgaaagcaa ttaatggtga tatgacaaca catatcatat tagatgaaat
    13081 tagcgcaaaa gagattggcg aaactttaat agcatgggct aataaagagc tgtaaaagat
    13141 aattttaaaa taaagtggcc aattgactac aagtagtctg tatagtataa taaattatca
    13201 aaagtgaggt atacatgatg aaatcaaatt ttttgggata tgtaattgca atcaacatgt
    13261 ttaaaggggg agttggtaag tcaactctta ctcagctaat ggctgatatt ctatcggcta
    13321 gaggctttaa agttttagtt attgatgttg atccacaagg aacagtaact aaaaagtttc
    13381 aaagacaata tgatttaaaa gttgttggga aaatgacatt gtttgaagca gtaattaata
    13441 tgtctttctt agactgcatt tctcctgtta aagaaaactt agatattgtg cttggttcag
    13501 aacaaatgtc aaggtttacc aaagtattaa gagatatgtg gagaagtact ggttttactg
    13561 atgaaggtga agaagtcttt tggaatttgt ttaattatat gatcggtaat gataatttaa
    13621 gagatcaata tgattttatt ttgtttgaca ctattcctac agtttcagaa tttacagata
    13681 acgtttacgt ggcgagtgat tttttagtaa ttcccacgca aactgagcaa gattctatag
    13741 acaacttgca tagtacggtt actaattatc aaactgctgt aaaagaagta aacggagatt
    13801 tagaaatttt aggtattgtt ccatatctag tggatactaa attaaaaaca ggattagaac
    13861 ttttatcaga acttcaagaa gaatatggag acttagttta ttctcatatt gtttcagaac
    13921 gaggcgtaat taaacgttgg ggtagagaag gactaaataa atctatccca cattctaaaa
    13981 ctgcttatag catgtatgta gacattgtag aagaaacgtt agtacgttta gaaaaagcta
    14041 caggagaaaa tattttagaa cgtgtttagg aaaggtgttg catgatgtcg aaaactaaaa
    14101 aagtaactag tgccggcagt aatggttcgg gtaatccgtt cggaataaaa agcggtagtg
    14161 gtggatttaa tataggacaa caaatgttaa aaggtaaagc tggcattaag tctactggtg
    14221 ataaacggaa atctgttagg gtaccagttg aattatacga tgaagttgtt aaagatttat
    14281 catacggggc taaatattca ttaatgacag aaattttagc tttattttta aaagaatttg
    14341 aaacacatgg aaatagtata gttactgatt ttggcaagtt gaaaacgaga ccagtaaaaa
    14401 tttctgaaga agcctttaaa gttttgaaag ttataaaatt tgaaacagat caatctaata
    14461 ctaatattat tgcttcagct ttgacatggt atcttgataa aagaaaagga aaagcataat
    14521 gaaatgaggt aagactatgg atcataaatc aaattattca tacaaagaaa attatatttg
    14581 gaagtatgta gtagccgttt ttctactggt aggtggaatt attgcattgg ttaaatgggc
    14641 tataattcct aatgctttag tatttatcaa ttggtttgtc cctaattttt ggagtttttt
    14701 tggctatata ttgataatct ggtttatgtt taaagtactc tcttttttct ggtcgcctgt
    14761 atattctttg cttggaaagt ggtcgtacct aattttaata gcgacagcta tttatatcat
    14821 tatgcagatt aactaattat gcagatttta gaagatatta aaatttattg gaaaaggata
    14881 ataggctata cattagttct tcttgtagta aatttattcc tttcgatgat agcgttggtc
    14941 tttgttaatc aaagaaaaga caatgtaact gaattagtaa cgtttatttc gtttttcata
    15001 agtgggattt ttgctccttt ctttgaagaa accttaatga gaggaattat ccaaaaccaa
    15061 ttggaattaa aaacaaattt gtctacaaga agcatatata taattgttgc aattttgttt
    15121 agtctatccc acttaaattt atattttatt ccttatttta tgacgagttt aattttgtct
    15181 tttgtctatg ataattcaga taaaaggctg attgttccgg ttactattca ttgtttttac
    15241 aatttaattg taatactgtt aagtgtagtt ttttatactt gaaaaaaata gacgaaaacc
    15301 taaaaataca aggtttttat ctattttttt tattttgttt ttatctatca ggttgataga
    15361 aattgtcata aatgatataa ttaagtatga actgatttag taaaaaaatt aaaggagtga
    15421 tagctgttgg caagatcaag aattgctaaa attgaacaga ctaaaaagga taaaaaaatt
    15481 tctaaaaata aaacaaaatt ctttaaagct gaagatgtta agaggatagc atggtctgtg
    15541 aaaacatttg atgaagatcg ttatgttaaa gtttcaatga atggtctatt tgtacagctt
    15601 ggtaacttca gtaattttaa aacgattgat gaactttatc cggaaataag aaatattgtg
    15661 gatagtaaag gtttaatcgt ggctaaacgg aaatcaagaa actctggtct tgcttactgt
    15721 aaaaataaag ggatcatgat tccggcttaa atcagtgaaa ttgttctttg aaaacttaat
    15781 actactcgta ctgtagcaat gttgctatgt tacagataaa agcgtaaggc taataagcaa
    15841 aatagcaacc aagcatactg ttatttcctt tgccaagagg tatgtaaata cgttagcaat
    15901 agaagtgata aggttagtat ttcacgccaa aaaatacaaa agtcgatcca ttgaaactac
    15961 taattatgag gtggcgtaaa tggtaattat tcagaatttc tatctaggaa gaacctatga
    16021 aaaccgtcct agagatcaga atattgacac taaaaaattc agcacagtaa taaatgtgct
    16081 agatacaggg taactcttta ctacaagaaa tgtagtaaag agttgttatg attctttatt
    16141 acatttcttt ctaaaggaag tgtgataatg aaaaaactaa aactgattag cattgctcta
    16201 ttcagcctaa tcactatgtc aacggctcgc caatcaattg tttcggctga aggaacgtct
    16261 acgaaagcag tagtagaaga gtctggcgat aaaaaagttg ctatagacga caaagtgaaa
    16321 aacgaagcgg aaaaagcgac tgtggttcaa ggggcagacg gcttgatgta tgtggtcgtt
    16381 gatgaaaatg atcctgaaaa attgctagaa gctgaatctc ctttgaaaaa tcaacaagaa
    16441 aataccggcg ctgttgaagt cggtgttgat gaaaagagac aagaaaaggc tgataaagct
    16501 aacgaaaaag tgaaaaactt tgacgatatt gattacaacg atattagttc gttgcagtca
    16561 gcgttgaatg atccgaaatt ccctttttta tcggaagatg aattaaatga aaaattgcaa
    16621 aagacaaaga atacatttga gtctgaagcg ctaatgaaaa agacaagggg atatttcagt
    16681 caaaggattt tagagttaag caataattta aatgacgtac cgaaaagagc gaaagtgagt
    16741 cggcatgctt tagttagtaa ccgtgcttca caagtaagtg aaacacgaat aaaccctcca
    16801 aatggttatc cgtgggtgat ttatttaatt aggagcaatg gttatagtta tacagaaaat
    16861 tatgctatta gaagaactat tgatgggaaa gaagcttttt gtttacaggc cggtgtacaa
    16921 gcgacgaata catacactgg ttcaacaagt ggttatcttt ctaattcgca aagagaaatg
    16981 attagttcga ttttatcagc atataaagaa ttaggcggtt gggctaataa tgatgccgga
    17041 aaacaaagat ggatagcaac tcaaatgctt gtttgggaaa acagtaatga aaagcctaaa
    17101 agtataactg ttacgccaaa ttggaaaaac ggagctacta agtggaaaaa tgatttagtg
    17161 gctcgtgcaa atgctattag agtaagaaca aaatgggatt ctacgtctaa gacggttgat
    17221 gagggagata aagcaacctt taaagtaact aatacttatg gagatcataa tttgcatgtg
    17281 aaatctgtat ctggtggcgg caaagctacc ataagcaatg gtactttaac tgttgatact
    17341 acaggagcca ctggaaacaa aatcactgta gatatgatta aaggttctca cccttcagac
    17401 gcgcacccta caacggtatg gagtaatggt tcttatcaaa agcttgttac cggttctttt
    17461 tacattagta cttctgttac ggtaaatgtt aagagaaaag gaactgttga agttttaaaa
    17521 gttgattcag aaacaaaaaa accattatca ggagcaacat ttagatttag ctactcaggt
    17581 aaaacaaaag atgttactac tgattcgaat ggtaaagcaa aattaactga aaggttgaaa
    17641 gctggaactg tagtaaaagt taaggagata aaagctccta atggctacca actagattca
    17701 agcgaatttt caattacagt taaagaaaat caaaatgtta ctaccacaag aaccaataag
    17761 aaatccacag gttctgtaga aattgaaaag ataggcgatt tagatggatt aggactaccg
    17821 aatgtagtat tcacgattta taattccgat aacaaagttg taaaagataa cttaaaaaca
    17881 gatagtaatg ggaaaataaa agtcgatgaa ctgcaatttg gtaaatatta tgcagtagaa
    17941 aaacaaggtg ttactggtta tgatcccgat ggtaaaaaat acaattttga aataagtcaa
    18001 gatactcctt ttacaaaacc ggctaaagtg aaagtaacaa atatttatga agtatccata
    18061 agcgaaaaag tatcggacat gaatgaaagt caggtattaa aaaataattg gtctgctgat
    18121 ccaaacgatt atcttcaata tgacattaca actggaaatc taaaaaaatc gggagaaaga
    18181 aacgtccaaa gtttcattat cgaagggttt tacgacaata agaatgtaga tgtgtcaaaa
    18241 agtgaagtat atgttggatc aacaaatgta acaaactctt ttgacattaa taacgatact
    18301 aacaatggaa aagtaattgc taaggcaaag acaagcgtat taacgaataa tgatttttat
    18361 aataagtcct ataatttacg gatcactatg aaaattaaaa aatctactct tgaaaacact
    18421 gaaaagcaaa aaaatttaac gattgttaaa aatagtaaag ttactgtcgg ttcaaaaatc
    18481 tctaaatctg ctagctctaa tgatgtagaa accgttttat attcaagaaa agttactatc
    18541 aatcatattg atgaaaaaga caaacacctt ttgaaacaag atattgatta taaatacgat
    18601 ggggaaacgt atgaatataa acctagaacc gatttatttg ataaagatgg aaacaattat
    18661 aaatctgatg taatccataa agggaaaatt gatggaaaag atatagtttt aaacactccc
    18721 tatcatattc cggtactttc tgtgaatgtg gatcggattc aaattgatac ggctagagct
    18781 gttaaaaatg gctatttacc tacaaaaata gaactaagta aaaaagaaga atatgaaaaa
    18841 gaattagaaa aaattgtatt caaagttaaa attacagata tagataacaa taaagttgtt
    18901 tatgatgaaa actttaaata caaagaattt aatagtcata tgtcgcttga tttacctaca
    18961 gaatatttaa caaagaatgt caatggccat tcaaaaatgt aggtttttgg ccatttagaa
    19021 atgtcgtttt ttggccacca aaaatgtagg tttttggccc ataaataaca aggttactta
    19081 tacgacaact ttttttcttt ataatcttta attcgataag actttccagt aatttgaata
    19141 atcttagcgt ggtgcactaa tcgatcgatt agtgcatttg ttaatttttt atcttgaaaa
    19201 gtttcagccc attgagacaa aggaatattc gttgtaataa tggttgattt tttttcatag
    19261 cgtagattaa ttaattgaaa gaataacttt gccccgtcag gggtaaaagg caaataacca
    19321 atttcatcaa taattagtaa atcgtaagaa gcatattttt ttaacaatcg atctaaattt
    19381 ccacgttgat ttgcacgatt tagtcgattg actaattcat tgcttaaagc aaaatggcaa
    19441 gaataacctt tggttatagc ttctagtgta atggcaatag ctaggtgagt tttccccact
    19501 ccgctatttc caataaacaa tatattatta tttgtatcca taaagcgtaa ggtacacaaa
    19561 tctaaaatct ctgttcgatt aatctttggt tgaaatccaa aatcaaagtc attgattgtt
    19621 tttttaaacg gtaaattcgc ctttcttaaa cgattttctt tttgtcgttc atttcgtgag
    19681 tttatttctt ccgtcattaa atgatttaaa gcttctacaa aactaagatt ttcttgaaca
    19741 atggtatcga gatattctgg taaaatttct ctcgtatgtt ttaaattcaa atcttctaat
    19801 ttgtttagta aatgataata attagacata atgattcacc tctctataat tcgtcatacg
    19861 cttgtaaatt ttcatcaaca aaacgttcta attcttcgtc ttctaaatgt ttaaaaacgt
    19921 cagaacgtag aatttctata tagtcatttc gatgataatt aagcaattga tgagtcatcg
    19981 tatgtgtgcg aataagagta gagtcatcgt aaatataaag tttatcttct tttacacgaa
    20041 gtgtaagaat tttaccgatg taatttactg gcacagaata tttattttga ttccattgaa
    20101 ccatcgcttc gttagatact tttcgtttaa tttgtttttc aacagaagca taaattttaa
    20161 gttgattcgc atcaaaagaa gatagttgtt ctttctcgta tgaccaacgg tcactaggac
    20221 attcatgaat tgcttgagaa atttcttcag tatttaatga atccatgatt gtttgaataa
    20281 ttttttctaa ttcgtgagta gattcaaatt cataattaaa tacacgaatt cgttcaattg
    20341 ttcgtgcaag agcttcaact tttccttttg tttgaggtcg aaaagcacga caagcaattg
    20401 gaagaaaacc tgcttctttc gcgtattgtc ggaaccgctc attaaaaata actttagaaa
    20461 attgactacg actacggtca acaacagttt tcatattatc aaaccatatt tctgtaggaa
    20521 cacctccggt gtattgaaaa gcattagtta agcaacgaaa taaagtagtt tgagaacgat
    20581 caaaagttag ttctaaatac ttaattctgg aatatcctaa aacgtaaagg aagatattac
    20641 aagaaataac ttcaccgaat ttattgacta atttgaggtc ttctttccaa tcaacttgag
    20701 cagataagcc aggagttgtt tcaacacgaa tcgttgcttt ttgtatttta tctgctttga
    20761 tattgcgaca atatcttctc agtgttcgtg cacttccacg atagccacgt ttagaaataa
    20821 aatgataaat agcagaagaa gaacaaccta aagctaattt gtcagcgata atatcttcaa
    20881 aatcactaac caaagttttt ttgtttttat tcttttgagc aagtaaaggt aatttattgc
    20941 ttattccagc ttcataataa cgttttactg tacgataatc gcaattgtat tgtttagcta
    21001 gagcagcata attaggtttt gtatgagtca taatgaaagg taacactcct tcataaatat
    21061 cttttctcaa tacactttcc tccttgaata aaataacaca aggaaagcgt accagatgac
    21121 gcataaacct acatttttat ttggccattt ttctacattt taacttggcc atttacaaca
    21181 aagaataaaa aaatcaatta tagtgttgat attcttttag tggataatcc agataaaaac
    21241 aagttcgaaa ctgaaactaa agatttaaga acgatcggtt atacttcttc tgaaaaagtt
    21301 ttaacaaaca aagatatgac aaataaccaa gtcaactatt cagcagtagt tagaacagtg
    21361 aaagaaagag agaataataa tccggtcaaa gagttcaatg agagtatcaa ttataaattt
    21421 actgatagca ctaaaacaaa aacaggatat ggtatatcag ttgatttaga gccaacatat
    21481 agaaatgaaa ttgggaaaca aatagatatg aaagtctata gttatccgat gaatgaattg
    21541 attgacagca aaattgacga aatctatcaa agtgggggaa aagaaacaga agttaagatg
    21601 gatcgcttaa agaatgatgg taaagttgag tcgggtatta gtactcaaaa tctttcattc
    21661 caatttccac atatgaatat cgaaagaaaa actggaaata tatatgctga cagtcaaaga
    21721 aatgatgaat ctattaagta tgaattgatc gatggcggta gaaaattata tgtgcctatt
    21781 tggtgtgatt tgggaaatta ttctattgct atcaaaagta gtaactttgg tggtaactat
    21841 atctcgttgg atatgaacca gaacatagat gtttatgctt atatgtatgc aacgatagat
    21901 agtgaaacaa aaaaagatga tgaattactg cttgaacctg tttatccaga gtctactcta
    21961 ccagatggtt ggagcaaaaa tgagattgat tggctgaata aataagtttg aaaagttttt
    22021 atctatctga tagaattaaa taatgatata gggatggtga tagaatgagg gttcaaagct
    22081 atggtaacag tgtctttgat gttaaaagag aatttcaaga ttctagaaga aaaaccaaaa
    22141 agctgatagc ttacttcatt ctatcagcta tcgttttcta tattgtcaag tttgttttgg
    22201 aattaaactc aatagtattc acagatatgg ttcataaaat gttaaatgga gaacctatta
    22261 atttcaactt tgattacatg cttgaaaaag cacaaagcaa tcaaattgtg gcttttatcg
    22321 tgagtatcta ttattttatt gaaaaatcta ttttttcttt aaaagatttt ttctttgaaa
    22381 tgcttaccaa actaacaacg ttttacaata aaatttcaaa aaattaagga ggaagtttga
    22441 atgttagatc aaaatacaga cagaagttgg tggatgattg gtgccgtgat cgtaggtgcc
    22501 atcttaatcg ggacagctaa agtagctttt cccgaagtgt ttaatatcgt cattacattt
    22561 ttcaaaaaca tgatccctac aaacttagga tagttccact aaaaaattca cgtagtttga
    22621 tgcgtagaaa aataataaag aatgaaaaag gctacataaa gtgatttatg tagccttttt
    22681 tttggatgga agtgatgtaa tgctaagaga tatgcccaaa atgattcttt taatgtttgt
    22741 tgggttcttt ttaattttag tgattgcgta ctattcatta aatggctata cacaagattc
    22801 gtctgtgaaa ggtatgcaag aaacgttaag aactacagca atagcaaaca gggatgatag
    22861 tattcgagta gaaaaaggac gatttgcttt aattaagtct gattttgaaa aagattttaa
    22921 agaaaaattt gggaaaagta aaaatctaaa taatgaaacc gctgattata aatttgatta
    22981 tcttacagat gggaaggacg gtattaaagc aattaaagtg aaggttgaaa gtggatcaaa
    23041 agaatatcaa gcaacttgta ttttaaatgt tgcaaatgat tagaggtgtg aaatttgcgt
    23101 gttgtagagg aaaagaagat ttctccaaaa atcgaggaaa ctcctaaaag taaaattaat
    23161 atcgcaagta ttattaaaaa aaatggaatt aaagaaaact ctgaagcgat agaaggaaac
    23221 cttgattttt taactaagct tttagaagaa gatagtggaa attatgaacc accaattgaa
    23281 gatgcaaaat taaatatgat tcgtgatgat ttaaccagaa actattccca agaagtttta
    23341 caagctttaa ctgatgatga aattaggcga aaacttgtac aagttgtttt gaaagaacat
    23401 ggtactttct ttaaaaaaga taaagaggtt gcagaatatg tagtacgtga atgtattgga
    23461 acagggatta ttgagcgaat tatgaccaac gatgaaatta cagatgttgg ttggaatggc
    23521 tctcaactta gcattgaaac taactctaac aaatggttgt ttgacggtga taaattaaat
    23581 atcacgcaag agtatattga acgtgttatt tctaaatatg ctgttgtaaa tgatcgggct
    23641 tttaatagtg gtaatcctat catggatgga atgtttaaaa atgttcgtat atcagctaca
    23701 cacatagata attcaccaga tggctctacg ttttctatgc gtgtggcaag gcctagatta
    23761 gcattgaata aagaaaactt tgaagatttt gctcctgatt atattttgga gctatttgag
    23821 aaaatgatga aaacaaaagc aaatatctgt atatcagggg agacaggtac aggtaaaacg
    23881 gaacttcaaa aattactgct aagttttatt gataatcatg atcgtatgat tatgattgag
    23941 gacgttcaag aaactcacgc taaagaatta tttccggata aagatatttt ctcttggatc
    24001 actactccca aaaaagcaat ttcagattta gtaaaagctt ctcttagaaa taatccacga
    24061 tggatcattg tttcagaaac acgtggttct gaagcttatg aaatgcttca agcggttttg
    24121 tcaggtcact atgttgtaac gacacttcat gcggttaatg cacgtgcgat acctagacgt
    24181 tttgtaaata tgtgtgcaag tggttttaaa ataaacgaaa atgcggttga ggaagacata
    24241 aatcgttatt tcgattttgg tgtccatatc aaaaaaacag tggttgatgg taaagtgatc
    24301 cgttatttga atgaaattgt tgaattttct gctagtgaac cgactacagt ttttaagcaa
    24361 aaatttatca atggaaagtt tgtcattgaa aatggtagtc tttccgaaga ttttaaagaa
    24421 aaaatggctg aaaaatttat tacgtttgaa tttcctgaag atcaaactaa tacagaagaa
    24481 tcacttcctg aagaagaaga ggtgatttaa gttgaaagta caggctatga caaagcgaaa
    24541 aggtctcatg gatgtttcgg aactaaacgc ctatcaagta gcgtatggta aaccgttaga
    24601 ttatagagat tatttgaact atgttggatt accttgtttg tttatgggtg gcttttcttt
    24661 tgctttatgt tactactggt ggattgcttt gatttgtgga gtaattggag gcgtatatgg
    24721 ctttaaagtt ataatgccta aaagtattaa acgctcttat gaattaaaga gtttacagga
    24781 aagaaataaa tttattaaca acatgactca aattttgacc gatgatagta aaacagttac
    24841 tagagcgtta tctatagcaa aacagcgaac aaaaggtgaa ttaagagaag atattcaaat
    24901 cttagaatca cgtttacaag gtgctgataa atttcagatt gctgaagctt ttcgtgaaat
    24961 caacaataaa tattatagtg atgttgtttt tacacaatac tttgagcaat tggaaacagc
    25021 tatttacgaa ggtagaaata attcagatac tatgaagcaa ataaagagct atcataatga
    25081 tgtgaaacgt aaaacagaag gttttatgaa aatcaaagac ggctatcttc aagatatgaa
    25141 gcaaatgatg tttattgtag ctgtttttat tggagctata acttttgctt ttggtttttc
    25201 tactttctat gaaggttttg ctcgatcacc tatcggatgg gtatttggat ctatttactt
    25261 tttgattatg gttagcttca tgaagtcatt tttcaaacat tattttgatg atgaaataat
    25321 gagtttgggg gtaagtaatt aatgaaagtt aaggctttaa aaaagaaaag aaaatcaagt
    25381 attagcccct tgaaaattac gaaaacagaa cataaaagct tttggattaa attactaggt
    25441 gatgtttatt atcaaaaatt acgtgaaatg aacaattcca atattgcgat tattaagtgg
    25501 caaaaacaac ggttaaaaaa gtcattgatt tttatcctag tcggattctt aatgtatcta
    25561 tttttaggga aagaatggta ttacttagcc ggtggaattg ctacttcagt aggtttttat
    25621 gtttggcaag caaaatcaat caatggtatc tataatcaat ttcggtttga acgacacttg
    25681 caatttagta aatttacacg gctattgatc ccctatctga aacaaaaaaa agatggcgga
    25741 aatttatatg gtgtttttgc aaaaatcgta aaaagactag attatgatac cgataaaaag
    25801 ctattaatga agcttatgca agaaatgaca gatcagccta acgatattag accttttacg
    25861 gaatatgcag aacagacttc tggaacagac atgtccgtat tatttatgtc tacagtttat
    25921 gatattcgac aaggttcggc agatttggat gttattgaag agttggatcg tttagcttct
    25981 gaagagttaa tggccggaat cgattcaatc attgactata agtcgaggcg ttttgtcttc
    26041 tttcctacaa aaattgcaat gacttcattt attttagttg tcggttttgc agtaggcgtt
    26101 cttttcatga acctaaaaga tattaacttt gggcaataaa ggaaaggaga aagtcaatgc
    26161 aagaacaatc cgttatggaa atcattaaat ggtttatagg tttaatgatg attatggcaa
    26221 tggtttctat aggacttttt tgtgttcaat tacaagatat taatactttc aagcaacaag
    26281 taaattatca aattgaacga aaaggaggtc tgacgacaga agctgttgaa gcaatcgaca
    26341 aatattcggc tactaattat gatagacgtt ttacaattga aagcgataag ttaaatcaga
    26401 aagtatctta tggagaagaa attgattata cgatcaaagc aactattcca attcaaattt
    26461 taccgcttcc tgatgtaact ttatcgttta acggaaatag tgtttctcaa ataagatgag
    26521 aggaaggata aaatgcaaga gaataatgtt tccagaataa tttatttgat tggcggattg
    26581 ataattttat ccttagctat tgccggtctt gtagagcatt ggccggaaat aaagttatta
    26641 ataagaaact tttacgacaa taaaatgatt aaaatgtagg aggtatagat ttggaagcaa
    26701 ttcaagaaaa tcgtaaggat aatcaagaaa caagtgaaaa aaacaaaaaa aaaagaaaaa
    26761 agaaagtaga taaatttacg gaaatgaccg ataaaatcat tgaaaagaaa ggacttaaac
    26821 ctaaagactt tgaccgtcaa gcaatcaaag aagcacgaca aaaactgtat gatgattttg
    26881 ttaaagaaaa catatcacag cttttagact aacatttagt ctaatggtga taagtcaaga
    26941 tgaaatttaa gat
//
LOCUS       DACEOE010000393        21835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.guided.032, whole genome shotgun sequence.
ACCESSION   DACEOE010000393 DACEOE010000000
VERSION     DACEOE010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.guided.032"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..377
                     /locus_tag="IW774_002950"
     CDS             <1..377
                     /locus_tag="IW774_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1155243.1"
                     /translation="AMKDLLAHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWE
                     WFIGKNRGALRLTTQPEPYSFERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEA
                     KLTEKHEKLIEQQHLAMEDLIT"
     gene            374..664
                     /locus_tag="IW774_002951"
     CDS             374..664
                     /locus_tag="IW774_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155244.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            661..882
                     /locus_tag="IW774_002952"
     CDS             661..882
                     /locus_tag="IW774_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155245.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            924..1703
                     /locus_tag="IW774_002953"
     CDS             924..1703
                     /locus_tag="IW774_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ1155246.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            1770..2264
                     /locus_tag="IW774_002954"
     CDS             1770..2264
                     /locus_tag="IW774_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1155247.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            2320..2958
                     /locus_tag="IW774_002955"
     CDS             2320..2958
                     /locus_tag="IW774_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155248.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            3030..3425
                     /locus_tag="IW774_002956"
     CDS             3030..3425
                     /locus_tag="IW774_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1155249.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            3409..5862
                     /locus_tag="IW774_002957"
     CDS             3409..5862
                     /locus_tag="IW774_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1155250.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            5859..7886
                     /locus_tag="IW774_002958"
     CDS             5859..7886
                     /locus_tag="IW774_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1155251.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            7883..8905
                     /locus_tag="IW774_002959"
     CDS             7883..8905
                     /locus_tag="IW774_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1155252.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            8922..9851
                     /locus_tag="IW774_002960"
     CDS             8922..9851
                     /locus_tag="IW774_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1155253.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            10096..10212
                     /locus_tag="IW774_002961"
     CDS             10096..10212
                     /locus_tag="IW774_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1155254.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            10228..12147
                     /gene="tet(M)"
                     /locus_tag="IW774_002962"
     CDS             10228..12147
                     /gene="tet(M)"
                     /locus_tag="IW774_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1155255.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            12245..12433
                     /locus_tag="IW774_002963"
     CDS             12245..12433
                     /locus_tag="IW774_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1155256.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(12491..12844)
                     /locus_tag="IW774_002964"
     CDS             complement(12491..12844)
                     /locus_tag="IW774_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1155257.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            13375..13797
                     /locus_tag="IW774_002965"
     CDS             13375..13797
                     /locus_tag="IW774_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1155258.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            13794..14024
                     /locus_tag="IW774_002966"
     CDS             13794..14024
                     /locus_tag="IW774_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1155259.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            14522..14722
                     /locus_tag="IW774_002967"
     CDS             14522..14722
                     /locus_tag="IW774_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1155260.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            14749..15942
                     /locus_tag="IW774_002968"
     CDS             14749..15942
                     /locus_tag="IW774_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1155261.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(16010..17575)
                     /gene="guaA"
                     /locus_tag="IW774_002969"
     CDS             complement(16010..17575)
                     /gene="guaA"
                     /locus_tag="IW774_002969"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1155262.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(17634..18557)
                     /locus_tag="IW774_002970"
     CDS             complement(17634..18557)
                     /locus_tag="IW774_002970"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1155263.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            18753..19358
                     /locus_tag="IW774_002971"
     CDS             18753..19358
                     /locus_tag="IW774_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1155264.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            19666..20682
                     /locus_tag="IW774_002972"
     CDS             19666..20682
                     /locus_tag="IW774_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1155265.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            20823..>21835
                     /locus_tag="IW774_002973"
     CDS             20823..>21835
                     /locus_tag="IW774_002973"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1155266.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMEL"
BASE COUNT         7293 a         3753 c         4341 g         6448 t
ORIGIN      
        1 aagccatgaa agatttattg gcacatcaac aggcggaaaa aacagccttt gaaattatca
       61 atcgctatat ccgctttgca gataaagacg acactaaacg tcggagtgat tggaaaacta
      121 atgaacgttg ggaatggttt atcggtaaaa atcgtggagc attacgccta accacacagc
      181 cagagcctta ttcttttgaa cgaacattga actggttaca tcatcaagtc gcccctacat
      241 taaaaatcgc aagcatttta gatgttctca acggtacaac cattatctcc accatgattc
      301 aggaagccaa actgacagaa aaacatgaga aattaattga acaacaacat ctagccatgg
      361 aggatttgat tacatgagcc ttttaacaaa agaattaaag aagttagggt ttcagtgtgg
      421 cattgagttt caagcgtata tacaaaatac agggaaatat acgtctctca ttattgaggg
      481 aaaacggcaa gctggtgata ccatctatac ctatgatttt tacaaggtga gattttataa
      541 taattatacc aatcgagtaa ccgtctatgg agagcattta accccttttc aattattaag
      601 gcgtgtaaaa agctatatct attatcgaga aaaatatctt aaggaaagga ggacaatcac
      661 atgaattttg ggcagaattt gtacaactgg ttcttggata atgcacagtc attggtattg
      721 ttagcgattg ttgtcattgg actgttttta ggttttaaac gagaattttc aaaactcatt
      781 ggcttcttga ttattgctct aatcgccgtc ggacttgtgt tcaatgcagc tggcgtgaaa
      841 gatgtgttat taaacttatt taaccgaatc attggtgctt aacagatatt tttcatgata
      901 aaataggaag tatatatttt gttatgaaag gatttatgaa tatgtttaca aatgatcaat
      961 ttaaatctct tttaattaaa acattagaaa gaaagagctc ttcaaattat tatgaaggag
     1021 gtaacgagat tgtttctaaa atgaaaatat cagaggtaac tttagatgag actgacgatt
     1081 ttacttatta taaaggatat aaaaagggct ggaatacgct ttgcacatat cttaaaataa
     1141 gagttccttt tgatgattta gacttttttg aaagtcataa agatttgatt acccagacag
     1201 caagctccat atatgataaa caaggtgata atgtattagt ggatactatt ttagtaccac
     1261 ttccagagaa ttacgaagta attaacttta gtcagttgaa aattagtgat gtggtttcac
     1321 aagctattga agacgctgag tcttttatga gtaatggtga atatcaacgt gcctttgaca
     1381 gagttcacac tgcttttcat ggatatttaa ttgaaatact aaaaaaatat gaaattacag
     1441 ttcctagaga cgaaaatcta tctaaattat atagtaggat acaacaatta attgaaaaag
     1501 aaattcaacc tactgaactt gctgatatag tgaagacaac gataaggtca tcaaatggaa
     1561 tgattagctc tcttaatgaa gcaagaaata ggcattcact tgcacaccct aatacaaata
     1621 ttattggaaa gagagaagca aaactaataa taggtatttc aagtacagtt acagattata
     1681 ttagcggata ccttgataag tagcgtcatt aactgattgc tacttttttt atgactattt
     1741 ttataaaaag gatgtgataa gaattggaaa tgaaagtcta tgtggcaaat ttaggacggt
     1801 ataatgaagg tgaattagtc ggagcatggt ttactccacc aattgatgag gaagaaatgg
     1861 cagaaagaat cggtttaaat gaggactacg aagaatatgc aatacatgat tttgagcttc
     1921 cttttgatgt ggacgaatac acgccgatta gtgaaatcaa ccgtctatgt gaagccattc
     1981 aagaaatcga aggtacacct atttataatg aactcaaaga aattcaaggc atgtggttta
     2041 gtagcttgga agaattactg gaaaacaaag aggatattca ttgttatagt gattgtgatt
     2101 cgatggaaga tgtcgcccgt tattacgtgg aagaaacagg acagcttggt gaagtccctt
     2161 ccaatttaca aaattatatt gactaccaat ctttgggacg tgatatggaa atagagggta
     2221 attacctagt tacctctcat ggggtatttg aatattgtca gtaagtttaa caatccgtgt
     2281 tataattaaa ttaataaata gcacggagag gtataaatta tggtaaatgt atttgaaaat
     2341 cttaaactat taaaactaga catgaaagat aaaggctggg tgatagattc cttttacttt
     2401 cgttacaaac agcaaaatta tattgtactt gttaagctat ttgaaaaaga ggaaaaagtt
     2461 ccagaatatg ctctgttaaa actagaattt ttaaaggaaa acgatttttc agatatgcta
     2521 gctgtctatg ctaattcggt aaaattatat acagacacga aaacaataag agaatatttt
     2581 ggtatcgaat acagtagtaa cttgggagat gttttatttc aatttagcca aactcttgct
     2641 cgatttattc cgacagaagt tagtgagaag aaaaatgaag accaaaaaga agccatgtgt
     2701 ttctcattaa gtcaaagtga ttcagaagat cccagaaaaa aatattgttt ttcagttaga
     2761 agaaatcctt taaagggaaa tggtgagctg ggaaaacgaa gtccttttaa tgacaataaa
     2821 acgagattgt atagacccag cttatatgaa cgattagggt cagataccaa tttaagtttc
     2881 agatattcaa tgaatccaga tgatgaagaa actgatgaag gtattattgc aaaatggaca
     2941 aaaaataaaa ttgaatagtt aaagcattga aagagcaatc acctattttg attgttcttt
     3001 ttctattttt acggaaagga cgtgaagaca tgaaaaaaat aagaagttac accagtattt
     3061 ggtcagtaga aaaagtgatt tatgcgatta atgatttaca actccctttc ccaattacct
     3121 ttacgcaaat gacatggttt atcgtttctt tgtttgtcgt catactcttt ggcagtttac
     3181 caccactttc tctgattgac ggagcactac tcaaatactt agggattccc attgttctca
     3241 ctgggttcat gtgccaaaag acctttgacg gaaagaaacc ctatggattt ctaaagtcta
     3301 tcattaccta tgctctacgc cctaaagtaa cctacgctgg caaacaaata cgattaaaaa
     3361 aagaaacaat gaatgaatcg attacgattg taaggagtga acaactagat ggcatatccg
     3421 attaaatata tggaagataa tctcgtattt aatcatgatg gcgaagtatt cgcttattat
     3481 gaattaattc cctataatta cagtttttta agttccgatg aaaaaattca agttcatgat
     3541 aatttctgtc aattgattgc ccaaaatcgt gatggcaaga ttcatgctct gcaactagca
     3601 accgaatcaa gtattcgagc cacacaggaa caatcgaaaa aagaaatcac tggacgatta
     3661 aaagagatag cttacgaaaa aatagatgaa cagaccgaag cgttaatttc tatgattggt
     3721 gacaatcaag tagactatcg cttttttatt ggttttaaac tctacctcaa tgaacaggaa
     3781 gtatccgtaa aaaatgttgg gaaagaaatt caaaaagcat ttgcggaatt ctttcatggt
     3841 gtgaatcaca aattaatggg cgattttgtt tctatgccta tggcggaaat cgaacgcttt
     3901 tctaagatgg aacagttgtt agaaagtaaa ttatccagac gctttcagat tcgtaaattg
     3961 gagaaagatg attttggtta tattattgaa catctttacg ggcaaacagg tgtccctttt
     4021 gatgactacg actaccattt accgaaacga tacgaagata aaaaagtgtt ggtaaagaaa
     4081 tacgatattc tcaaacccac tcgctgttta atcgaagaaa atcaacgcta tttacggatt
     4141 gaacaggaag ataccactac ttatgtggcg tattttacaa ttaatagtat tgttggtgaa
     4201 ttggatttcc ctaatagtga aattttttat taccagcaac agcagtttac tttccccgtt
     4261 gataccagta tgaatgttga aattgtcgcc aataaaaaag ccttatctac cgttcgcaat
     4321 aaaaagaaag aactgaaaga tttagatagc cacgcatggg aaagcgacaa cgaaaccagt
     4381 actaatgtgg tggacgcttt agaatctgtc gatgaattag aaagtaattt agaccaaacc
     4441 aaagaatcta tgtacaaact aagctatgtg gtacgggtat cagcaaagaa tttggacgag
     4501 ctgaaacaac gttgtgatga agtaaaagat ttttatgatg atctgtctat caaattagta
     4561 cgaccttttg gcgatatgct aggcttgcat aatgaattta ttccctcaag caagcgatat
     4621 atcaatgact atattcaata tgtcacgtcg gattttttgg ctggtcttgg tttcggtgct
     4681 acacaaatga ttggtgaaaa ttctggtatt tacgtcggtt acaatctcga tacaggcaga
     4741 aatatttatc tccaacctag ccttgcaagt caaggaatta aaggctctgt aacaaatgca
     4801 ttagcgtccg cttttattgg ttcattaggt ggcggaaaat cattctctaa caatatgctg
     4861 gtctattatt cagtactctt tggcggacag gcaaccattg ttgacccaaa agctgaacgt
     4921 ggtgaatgga aagaaacctt acctgatatt gcccatgaaa tcaatattgt taatctcacg
     4981 agtgagaacg acaataaagg gttacttgac ccttatgtga ttttaaaact cccgaaagat
     5041 tcagaaagcc ttgccattga tattctcacg ttcttaacag gtatttccag ccgagatagc
     5101 gagaagtttc ctgtcctgcg taaagctatt cggagtgtaa ctcaaagcaa acaacgaggg
     5161 ttgttattgg tgattgatga attgcgaaaa gaagatacgc ccatcagcaa tagtattgct
     5221 gaccatatcg aaagctttgt cgattatgat tttgcccatc tgttattcag tgatggcagt
     5281 gtcacccaat ccattagctt ggaaaaacaa ctcaatatta ttcaagtggc agatttggtc
     5341 ttacccgatt ccaatagctc ttttgaagaa tacactacca tggaattatt aagtgtggca
     5401 atgctgattg tcattagtac ctttgcttta gactttatcc actctgaccg ttccattttt
     5461 aaaattgttg accttgatga ggcttggagt tttttacaag tggcacaagg aaaagctcta
     5521 tctatgcgat tggttcgagc aggacgtgcc atgaatgcag gggtctattt cgttactcaa
     5581 aacgcggacg atttactaga tgaaaagatg aagaataata ttggcttgaa atttgccttt
     5641 cgttccactg acctagtgga aatcaaaaag accttagaat tttttggtgt ggacaaggaa
     5701 gatgaaaaca atcagaaacg cttacgtgaa ttagaaaatg gacaatgttt agtttcagac
     5761 atctatggtc gtgtaggggt tatgcagttt cacccgattt ttgaagagtt gcttcatgcc
     5821 tttgatacca gaccgcctgt gcgaaatgag gtggaagaat gacacaaaaa caaaagaaat
     5881 ttcttcacta tagttggata acgctccttt gtgtgggcgt tattttactt ttattaggca
     5941 cgctgggcaa tgcagtacaa gcgacaggct tagttgatga aaccattgat acaagcaatg
     6001 aatactccaa gtatggtttg aatcaatatc agcttgatta ttatgtggac aatagctggg
     6061 gctggcttcc atggaattgg agtgatggca taggacaatc ggtgatgtat ggattgtatg
     6121 ccattactaa ttttatttgg acaatttctc tttatctatc gaacgccaca ggttatttgg
     6181 tgaaagaagc ctatgcctta gatttcatta gtcaaacatc ccaagcaatt ggcgaaaaca
     6241 tgcagacgat tgcaggcatt acaccgaatg gcttttccag ttcagggttt tatgtcggtt
     6301 tcctccttct ttttattctc attgttggaa tctatgttgc ctatacagga ttggttaagc
     6361 gagaaacgac gaaagtgata cgagctgttt taaactttgt cgtggtgttt attctatcgg
     6421 cttcctttat tgcgtatgca cccgattata ttgcgaaaat caatgatttt tccaaagatg
     6481 tcagtaccgc cagtctgaat attgggacaa aaattgttct tcctaacacg gatagtcaag
     6541 ggaaagacag tgtggacatg atacgtgaca gcttattctc tgttcaagta aagcaaccat
     6601 ggctattgct tcaatatggc tccactgatg tagaagcgtt aggggaagaa cgtgtggaaa
     6661 gtttattgtc taccagtcca gatacaaaca atggtgaaga tagagaaaat gtagtgattg
     6721 aagaaattga agatcatgac aatacctatt taaccctacc gaagacgatt tccagattag
     6781 gaacagtctt ctttttgttt gtattcaata ttggtatctc gatttttgtt ttccttctaa
     6841 caggaatcat gattttttcg caagtgctat ttattatttt cgctatgttt ttgccaatca
     6901 gtttcctatt aagtatgttg ccgagttttg atggcatggg caggcatgcc attaccaagt
     6961 tatttaatgt gattatgaca agggctggta ttaccttgat tattaccgtt gcctttagta
     7021 tttccactat gctttattcg ctgtcagcaa gttctccctt tttcatgatt atgtttttac
     7081 aaattgtgac ctttgcaggg atttatttta aactgggcga tttaatgagc ctattcactt
     7141 tacaaagcaa cgactctcaa aacatgggca gacaagtgtt tcgcagacct cgtcaaatga
     7201 tgaatcgaca aaccagaaga tttcaacgca gtatgagtaa gatttttcgt ggaaaagcag
     7261 ggtcaacgaa gcaaaagaca atgacaacaa agcagaatca aaccaataaa gctaaccata
     7321 ctcgtcaaaa tgaacggact acaccaaaga aagaaacagc actttccaat gtgaaaaagc
     7381 aacaaggcgg tatcgggcaa cgattaggtg aaaagacagc ccatgtattg gacacgaaaa
     7441 atcgtctagt caataaaaga aagcaggcaa aacaacagat acaggcgaca ccaacccatg
     7501 tcaaatatgc ccttcatcaa ggaaaagaga atgtcactca taatgtaaca gactttaaac
     7561 aatctatggc agataccaaa gctcaacgca aacaacagcg tatgaaccag caggagcaaa
     7621 aacgaaagaa cattgctcaa aagcgtttgg agctggataa agcaaaagaa aaaagacgaa
     7681 aagaaaaccc atcatctcct gttcagaata accagcagca tgttcgcaag cgtcagcaaa
     7741 ccgtcacgcc tgttttgtca caaaacacta aacgtgaaaa tcatcagcga ccattgaaaa
     7801 aagagcaaaa acaaaagaca ccaccgaagc aattcaatca gaggaagcta aataaaagaa
     7861 cggataagcg aggacaacaa acatgaagct aaaaataaca gtgatttgta gtgtggtctt
     7921 ctctctcttc ttttttctct tattattcct agtcattttc tttgcagatg atacggacag
     7981 tggcgaaaac aacaaggatt cttctattcc acaaggcggt gtgaccgttt caccagaagt
     8041 gttggcacac cgtccgttga ttgaaaaata tggtaaggaa tatgggattg aagactatgt
     8101 ttcttatatc ttagccatta tgcaagtgga atcgggcggt acagcagaag atgttatgca
     8161 aagttccgaa tctttgggtt taccgcccaa cagtttgagt accgaagagt ccatcaaaca
     8221 aggggttaaa tatttcagtg aattattaac cagtgcagaa caacaaggag tggatataga
     8281 ttctgttatc caatcttata attatggcgg tggcttttta aattatgtga ggagtcatgg
     8341 gaaaaaatat acctatgaat tagccgaaca gttttctaaa gagaagtcgg gtggtcaaaa
     8401 agcagactat cccaatccta tagccatacc tgtgaatggt ggttggcgat ataactatgg
     8461 aaatcagttt tatgttcagt tggtttcaca atacctaacc gacacttccc caacagaatt
     8521 tgatggtgaa accgtgcaag tcattatgga cgaagccttg aaatacgaag ggtttcccta
     8581 tgtctttgga ggggcgtcgc ctaccacctc ttttgattgt agtggcttaa tacaatgggt
     8641 gtatgacaaa gctggcattt ctctccctcg tgtcgcccaa gatcagtatg acgctacgca
     8701 agaaatctct atggaagaag cccaagcagg cgaccttatc ttctttcatt caacctataa
     8761 tgcaggcaca tatgtgactc atgtggcgat atatttagag ggaaatcgct tttatcatgc
     8821 aggcgaccca atcggttatg gcgacttaag cagtcgttat tggcaagacc atctgattgg
     8881 ggctagacgt gttattcata actaagaaag gaatgaatca aatgattata aaaatcgagc
     8941 gaaaagaaaa gaaggagaaa ccaaagaaat ctaaaaaacc gagcaagcaa agaaaagttc
     9001 cgatgattaa agttggcacg cataagaaat taacctttgt tttatgggtt ttattgattg
     9061 gtagtgtcgg atttggaatc tataaaaatt ttactgccat tgacactcat actgtccgtg
     9121 aaacagaaat cattaaacag cagattgtcg ataccaacca agtagaaagt tttgtgaaat
     9181 cctttgcgaa agactacttt tcgtggcaac aatcccaaga agccattgat aaacgaaatg
     9241 aaaaattgac ccattatttg acggaagaat tgcaggtatt aaatgaagaa atgattcgta
     9301 aggatattcc tactagttct agtgtgaacg atatacaggt gtggcaggtt tctcaagtga
     9361 atgagaacac ctttgaggta ctgttttctg ttgaacaagt tattaccgaa gacaaagaca
     9421 aggaaactat atcgtctagc tttcatgtgg ttgtccatat agatgaatca gataatatgg
     9481 ttattatcaa aaatccgaca atgagtaaga aacctcaaaa atcggactac caacccaaac
     9541 agctagaaag tgaccataca gtagatacag aaacgatgga cgaaatcact agctttttag
     9601 aaacgttctt ccagctctat cctaccgcca cagaaaaaga attgacttac tatgtaagta
     9661 atcatgtgtt acctatgatt aacaaggaat atgttttcga ggaattggtg aatcctatct
     9721 ttaccagaaa agataaccaa gtaatcgtga atgtcgctgt gaaatattta gatcaagaaa
     9781 caaaggcaac ccaaatctcg caatttgagt tgatattaga aaaacaagat aattggaaga
     9841 ttgtaaaata acaaatattg gtacatgatt acagatactt tgtaatcatg tactcttttt
     9901 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtgacta
     9961 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    10021 cgggaacaaa taattagatg tcctttttta ggagggctta gttttttgta cccagtttaa
    10081 gaataccttt atcatgtgat tctaaagtat ccagagaata tctgtatgct ttgtatacct
    10141 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    10201 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    10261 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    10321 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    10381 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    10441 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    10501 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    10561 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    10621 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    10681 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    10741 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    10801 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    10861 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    10921 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    10981 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    11041 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    11101 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    11161 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    11221 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    11281 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    11341 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    11401 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    11461 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    11521 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    11581 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    11641 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
    11701 tggaatgtga cggattgtaa aatctgtttt aagtacggtt tatactatag ccctgttagt
    11761 actccagcag attttcggat gcttactcct attgtactgg agcaagcctt tagaaaagct
    11821 ggaacagaat tgttagagcc atatcttagt tttaaagttt atgcaccaca ggaatatctt
    11881 tcacgggcat ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac tcaactgaaa
    11941 aataatgagg tcattattat tggagaaatt cctgctcgat gtattcaaga ttatcgcaat
    12001 gatttaactt tttttacaaa tgggcttagt gtttgtttag cagagctaaa aggatatcag
    12061 gttaccactg gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat agataaagta
    12121 agatatatgt tcaataaaat aacttagtgc gttttatgtt gttatataaa tatggtttct
    12181 tattaaataa gatgaaatat tctttaatat agatttgaat taaagtggaa aggaggagat
    12241 tgttattata aactacaagt ggatattgtg tcctatttgt ggaaataaaa caagactacg
    12301 aatacgagtg gatactatac ttaaaaattt ccctttatac tgccccaaat gtaagaacga
    12361 aactttaatt aatgttcaaa aaatgaatat aataacaatc aaagagccag acgccaagac
    12421 gcagagccga taatttgaga aatgaaactc tcatcttatc ggctcttttt gtttatctga
    12481 attttactga ctagccttca atatttcttg cttcattgat accttttgct agtgcttcca
    12541 ttaaggatag ttctttgtct gtaaagctat ccatgtattt ttctacctgt aaccttcgag
    12601 tgcttttaat caaattatct gtaggtaaga aaaattcatc aatagataca tggagtaatg
    12661 atacaagatc atacagcact tgtgtacttg ggtgttgtcc tttgttttct atatttgtta
    12721 aataacgtgg gtcaatttca atcattgttc ccacttgttc acgagtgaga ccccgtttca
    12781 ttcgagcctc tttaatcgct aaaccaacgg ctctaaaatc gtatttatct tcttttttac
    12841 gcatactaaa tcacctctat acattttact gttcctatat aaccataaac aggtatagaa
    12901 aaacatgtta tatagtttat gagttcctat agaacactgt ggaatttatt aatgcgatga
    12961 atccaatttc aaatagaaag gtcgctgatt ttatttacct atacaacttt ataaaattta
    13021 gtcatactcc tagaaagtag ataaaattgg cgacttaatg gagggaaaag tcgccatgag
    13081 aaagataatc tgtcatctag ttctcttaat taagttctta tcaaaaaaac ttatccacct
    13141 gtactggata gatatgacca tcaatgtggt tttgcagatt acatagatag aaaaatcaaa
    13201 gcatatgaac agagaaaaca atctgtttat gtgctttttt ggtcacttaa taaatatctt
    13261 ttaggatttc tcttcattta tgcaacaaat ccccctttcg tgtcggggtt tagtatgaag
    13321 ggagataaag aagcacatgc cactcccttt cacgacagaa agggggtgaa aagcatgaaa
    13381 ccatcttctt ttcaagaaac catagaaaat cagtttgact atatctgcaa acaagttatt
    13441 gaagatgaac gaaaagatta cttcaaacat ctagacagac tatcagcaaa agaagtctcg
    13501 ttttctgata tagataacta ccttgtcaac tctttttcta ctgttgatac gtacgtcact
    13561 gatttccaac tatttaaatt atttggatta agaatcaatg ttgaaagtga tttattaagt
    13621 gaagccctgc gtcatctttc agagaaaaaa cggaacatta ttttacttca ttactttatg
    13681 gatatgagtg atgtggagat tgcggagtta ctaaatttga atcgttcgac tgtttatcgt
    13741 catcgaataa gtggactagc catgattaaa gaattcatga aggagtgtaa agaatgaaaa
    13801 cagcttatcc aagggttccc ttccccctca ttgtaaaggc gacagatggc gacgtggaag
    13861 caattaatca gattgttaaa cattatcgag ggtatacctc aaaacgttca ttacgtcgca
    13921 tgacagacga atatggaaat tctcatatgg ttattgatga aaccctacgt ggcagaatgg
    13981 aaacgaaact catctctaag attttatcgt tcgaaattaa atagtcccat tttttcgtgg
    14041 aagcgtgtcg aatcgtatcc acgcttcccg atttgggagg tttgttattc catcaaagca
    14101 tattgtgtgt tcagtaggtt ttgataggct aacaagccaa acgttctttg acaaccaaat
    14161 aaaggcaatc gaatacgttt cggtaagaaa agagctgatg aactaacacg ccatgaccta
    14221 caaaattagt gagcgattca tgttagtaat tcgaaagtaa tctttagcag gattgctgcc
    14281 atgactttct tatcgtgata atgatactcc cgtatagcca tagtccgagc gttagaagcg
    14341 tcacaggcaa tgggtacggc tacatgagaa ccatgcagaa gtgaaattcc tgtgagcttt
    14401 gctagagctg ttcgattgct aagatattct tctcttgtca gtgtattatt ataaaactat
    14461 ttgatataat tataggtgaa tattggagtt ttcctagcct ataattggga aaggagtaat
    14521 aatgataacg gtcacaaaaa aggatttagt tgagctagga tatggaactt ctttttcagc
    14581 agacatcata aaaaaagcaa aagaattgat gattacaaaa gggcatatct actatcaatc
    14641 tcgaaagcta gatagagtcc ctcgtgaagc tgttgaagaa attctaggaa ttcggttttc
    14701 aaatgaaaac aggtctgagt agtttttgca cagtaaggag taaaaactat ggttaaagac
    14761 ccaataaaaa aagcaaagaa tggaacctat tattttcgtg caaatctagg ttatgattct
    14821 aaagggaaaa aaattcaaaa atatcgtagt ggattcacta ctaaaaaaga agcaagagaa
    14881 gaatattcga aactgctatt gatgaaacca gaagaattaa gtgagaataa ggataaaatg
    14941 acgtttaaac attatatctt tgaaattttc cttccatggt ataagacaca ggtaaagctc
    15001 agaacatatg aaaatcgctt accaactatt aagaagcatt tctcttattt tgataaaatg
    15061 gctgtttccg atattgagcc tattgatgtg caaaattggc aactaaaatt atcaaagaag
    15121 tgtaagtctt cttatgtaag agctgttcaa ggactatttt cagttgctat ggatcgagca
    15181 attgtccttg ggataaccac cacaaatcca tctaagatta ttggcaatgt gaaaaagcaa
    15241 aagtctaaaa ttgaattttg gacaaaagaa gaatttgaaa aagtaatctc tctaatttac
    15301 aaagaagatt actatcaaca ctttttattt atctctctgt ggtttttatt tatgactgga
    15361 atgcgaattg gtgaagctac tgccattcaa tgggaagata ttgattttga ctcaggagta
    15421 ctaaccatca acaaaacact ctattacaag aatcaatcga attatagatt tgttgaacca
    15481 aaaacaaagg cgagcgttcg tcatattgta ttagataaat gcacattaac ttatttatcc
    15541 gaatggaagg aagtacaaca aaatctaatt cagacagact ttgtaatgag ctataacggt
    15601 attcctaccc aaaaacatac actatcatat gctattgaac gctatgctaa gctggctggt
    15661 atccatcgaa tcagaatcca tgggttacgg cattctcatg cttccttgtt aatcagtatg
    15721 ggagaaaatc cacttatcat taaagatcgt ctaggacacg aagatattga gacgacttta
    15781 ggaacgtatg gacatcttta tcctaacagt aatttcgagg tagctaacaa attaaatgga
    15841 gccatttcct ttaaagaagc aaaacaaaat tatgatactt caccaaagaa tcaatttact
    15901 gtagactact tgcgaaacaa agaaatgaaa aaagtgcaat aaaagtgcag taaaaagata
    15961 agaagagcca gaaacccttg gtataagagg atttctagct ctcctacaac tattcccact
    16021 ccactgttgc tggtggttta gaagtgatgt catacacgat acggttgacg tgggctactt
    16081 cgtttacgat acgtacagag attttttgca agacttccca tgggatacgg gcaaagtcag
    16141 cggtcattcc gtcgattgaa gttacagcac ggataccgac tgtgtagtcg taagtacgtc
    16201 cgtcacccat aacaccgaca gagcggatcc ctggaagaac agtgaaatat tgccagatat
    16261 cacggtcaag tccagcagct gcgatttctt cacgcaagat agcatcagat tcgcgtacga
    16321 tttctagctt gtcttcagtg atttcgccaa gaacacgaat accaagacca ggaccaggga
    16381 atggctgacg ccaaacgatt gaatcaggca tgcctaactg agtccctaat tcacggactt
    16441 cgtctttgaa taatgtgttt aatggttcga tcaattcaaa ttgcatatct tctggcagtc
    16501 cacctacatt atggtgagat ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt
    16561 cagtatacaa cgttccttgt gctaggaaag agacgccttc ttcaccagca agttttgttg
    16621 cttcgtcatc aaaaacatat acgaattcat tcccaatgat tttacgtttt tgttctgggt
    16681 cagatacacc tttcaacttg tctaagaagc gatctcttgc atcgactttg atgatgttca
    16741 agccgaattt tccgcctaga ctttccatta cttgttccgc ttcgcctttg cgtagcagac
    16801 cgtggtcaac aaagatagat gtcaattggt caccgatcgc tttttgtaaa agaaccccaa
    16861 cgacactaga atcaacgcca cctgaaagac caagcaagac ttttttgtca ccaacttgtt
    16921 cacggatttt ttctacttgc atatcgataa agttatccat tgtccagtta ccttcacagt
    16981 gacaaacatc aaaggcaaag tggcgcagca attcgattcc gtattcggaa tgacgtactt
    17041 ctgcatggaa ttggatacca taaaaattac gtgttgtgtc ttggatagaa gcaatcggac
    17101 aatctttgct tgttccgact gtttcaaagc cttcaggaac tttagttacc aagtcaccat
    17161 ggctcatcca tactgtttgt tgttttggtg tttctttaaa caagacagct tgatcgcttg
    17221 ttacttctaa caaggctttc ccatattcgc ggtttttagc aggctctacg gaaccgccaa
    17281 gtttgtacgt gatcaattgc ataccatagc aaatccctaa tacaggaata cctaaattga
    17341 agatctcagg atcgatccca aatgcattct catcatatac actgtttggt ccgccagaaa
    17401 gaatgattcc ctttggattc atcgctttta cttcttctgc agtaatgcgg tggctcaata
    17461 attcggaaaa gactccaaga tcacgaatac gtcttgtgat caattggtta tactgactac
    17521 cgtagtctaa tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta
    17581 ttacccctat ccctttaaat tttatctcaa aatagtcaaa ctattttaaa atgctaatat
    17641 tttcgcatga aaatcgaatc gatgatatga ttttcagttt tatggagaat tatatcggct
    17701 cttcctcgtg taggcaagat atattcctgt aaatttttca aattcacatt tttccataca
    17761 tcttttgcca ttttaaaggc ttcttcacga tctccgatag caaaacgata ataataattc
    17821 tctggatttt ggaaagccgt atccagtaac gcaccaaaac gatccaaata ccatttttcg
    17881 atcagcacgg gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt
    17941 tgctgattcg caggtaattg aagcgtattg atcccctcaa cgatcaagat atcaggctgc
    18001 tggatcaatt cgtacttgtc tggaatgata tcatacacat catgtgagta aacaggtact
    18061 tttatctctt cctgaccaga ttttacacgg ttcaagaaat cgatcagcat ctccatatca
    18121 taactttcag gaaatccttt acggtccatg attccacgct cttttaatat acgatttgga
    18181 taaagaaatc cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt acgagacaaa
    18241 atcgtctgca gcagtctggc tgttgtactt tttccaacgg ctacacttcc ggcgatcccg
    18301 ataataaaag gcggtacttc tacgtattta tgaagaaata aacctttact caagaacaaa
    18361 gattcaaatt ctttcatata aagataaatc agatgagcaa gaggtacata gatatctcga
    18421 acatcctgca tggaaatgcg gtcattcaaa cttttgatat tatctagttc atcttgtgtc
    18481 aaaggtgctt gcccattacg ataaaattct cgccattctt ctcttgccac acggtagtag
    18541 ttcattggat cattcatatt agcctcctgc gttctcccat caatggaact attttaacac
    18601 aaaataaacg caaaccccac cacttaagag agattgccga tctttttgta taatctttat
    18661 aatagaaaga aacggagtct tattcttgac ttgcgcacgg atacataaaa ggatagactg
    18721 ttgtcatgaa agaaatgaga aggatggaag acatgacaaa tcattactat tctgaacgcc
    18781 cagatacggc gcacgatttt gaacaatggg cttttgagtt aaaaggcaga acctttcaat
    18841 ttgtaacaga ttctggtgta ttttcacgtg aaacagtgga ttatggttca cgtgtcctga
    18901 ttgatgcttt cgattgggaa gagttacctg atgaaggaaa gatcttagat gtgggatgcg
    18961 ggtatggacc gattgggtta gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg
    19021 acattaatga acgagcggta agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac
    19081 atgttgatat ccatcaatcg aatatctatg aagcagttca tgaagaaacc tatgcagcta
    19141 tcgtaagcaa tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg
    19201 catatcctcg gttgaagaag ggcggtacac tgacaattgt gatccagaag aaacaaggag
    19261 cgccgagtgc gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc gtcacaaaag
    19321 ataaaggata ttatatcatc aaaagtgtga aagaataact agctgaaaaa tagtggagcg
    19381 acgaaagagt ggaactgggc gtgcatgtac gggtacgtat cagttctttt tttgttgaga
    19441 agtttttaaa aacaaagaac tattttttct tcaaaaagaa atagtaggaa cagagtcgtt
    19501 gttttttacg ataaaataaa taagatacag aacttgtata gaaggctgcc aagtcggtag
    19561 ataaaggagg tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg
    19621 ttttggtaca ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc
    19681 agagatatcg cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac
    19741 aaaggaagca gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat
    19801 cattataagc ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga
    19861 atgatcgtcc ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc
    19921 tatttgaatc ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat
    19981 gagatcaagt atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca
    20041 ccaaatattt tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc
    20101 attttaatcg atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat
    20161 gagggcgcct atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg
    20221 ctgaaggaaa ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg
    20281 ctgaaagatc atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc
    20341 caaggtggat atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt
    20401 tgcgggaatg atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa
    20461 atcccagaag atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt
    20521 ccgcaattga cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc
    20581 ctgatcgata cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa
    20641 ttaattattc gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt
    20701 atgtaagcat ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta
    20761 aagtggttta cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg
    20821 caatgcgaat ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg
    20881 aaatcgagta catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg
    20941 cattattgat ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat
    21001 tggcgattgc taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag
    21061 ataagcattc gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag
    21121 ctagtgtcgg agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta
    21181 cactagacaa gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg
    21241 tacgcattgt taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg
    21301 cggacaaaaa attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga
    21361 ttgccagttc gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg
    21421 tgaccactgg agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga
    21481 ccatgacgag cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt
    21541 cagaacctct tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat
    21601 tacaaggaaa tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg
    21661 ttgttcttgc tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc
    21721 tggaatcagg taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc
    21781 cagcgatcgt ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagct
//
LOCUS       DACEOE010000394         6747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.guided.142, whole genome shotgun sequence.
ACCESSION   DACEOE010000394 DACEOE010000000
VERSION     DACEOE010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.guided.142"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            109..192
                     /locus_tag="IW774_002974"
     CDS             109..192
                     /locus_tag="IW774_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1155267.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IW774_002975"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IW774_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1155268.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IW774_002976"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IW774_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IW774_002977"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IW774_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IW774_002978"
     CDS             2348..2593
                     /locus_tag="IW774_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1155269.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IW774_002979"
     CDS             2696..3565
                     /locus_tag="IW774_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1155270.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="IW774_002980"
     CDS             3546..4280
                     /locus_tag="IW774_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1155271.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW774_002981"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW774_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="IW774_002982"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="IW774_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1155272.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="IW774_002983"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="IW774_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1155273.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2365 a         1013 c         1407 g         1962 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgattagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctggctctt
     6721 tgtcaaatga tgttggtaaa gagaaat
//
LOCUS       DACEOE010000395         4897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.guided.170, whole genome shotgun sequence.
ACCESSION   DACEOE010000395 DACEOE010000000
VERSION     DACEOE010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.guided.170"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            112..840
                     /locus_tag="IW774_002984"
     CDS             112..840
                     /locus_tag="IW774_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1155274.1"
                     /translation="MNKGAVGADAARTMKRSKAIVNRMETQISEKEKLLKDIEYIDSL
                     TMNSQASHHKRLLSVEDLQLGYENLLFEPIHFTIEPHQRVAISGPNGAGKSSIIHYLL
                     GAFNGKVIGEKYQPKHLSISYASQNYEDNRGTLAEFAEKNQVDYQAFLNNLRKLGMER
                     DVFHNKIEQMSMGQRKKVELAKSLSQPAELYTWDEPLNYLDVFNQEQLEQLILNVKPA
                     MLLVEHDQTFLDKVSTEIISLERI"
     gene            907..1434
                     /locus_tag="IW774_002985"
     CDS             907..1434
                     /locus_tag="IW774_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1155275.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            1645..2706
                     /locus_tag="IW774_002986"
     CDS             1645..2706
                     /locus_tag="IW774_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1155276.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            2709..3605
                     /gene="murQ"
                     /locus_tag="IW774_002987"
     CDS             2709..3605
                     /gene="murQ"
                     /locus_tag="IW774_002987"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1155277.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            3619..>4897
                     /locus_tag="IW774_002988"
     CDS             3619..>4897
                     /locus_tag="IW774_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1155278.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGA"
BASE COUNT         1634 a          859 c         1130 g         1274 t
ORIGIN      
        1 gaagtcagta ggctaaagaa aacagcagct gaaaaagccg aatggtctcg ttcccgagaa
       61 ggagataaaa caaagaaaca agtcggattc atcgatactg aatctagacg agtgaataaa
      121 ggagcagtgg gtgctgatgc tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg
      181 atggagaccc agatcagcga gaaagaaaaa ctattaaaag atatcgaata tatcgattct
      241 ttgacgatga atagccaagc gtctcaccat aagcgacttt taagcgtaga agatcttcaa
      301 ttagggtatg aaaatctgtt attcgagcca attcatttta caatcgagcc tcatcagcgg
      361 gtggcgattt caggtcctaa cggtgcagga aagtcatcca ttatccatta tcttctgggg
      421 gcattcaacg gcaaggttat aggagaaaaa taccagccaa aacatctgag cattagttat
      481 gcaagccaaa attatgaaga caatcgagga acgttggcgg aatttgcaga gaaaaaccaa
      541 gtagactacc aagcattttt gaacaacctc cgaaagcttg ggatggaaag agatgttttt
      601 cataacaaga tcgagcagat gagtatgggc caacggaaaa aagtggaatt ggctaaatct
      661 ttatcacagc cagctgaact atatacatgg gatgaaccat tgaattattt ggatgtcttc
      721 aatcaagaac aattagaaca actgatcttg aacgtgaaac ctgccatgtt actagtggaa
      781 catgatcaaa ccttcctgga taaagtatct actgagatta tttctcttga gagaatctaa
      841 cccttgtaat ctcaagaaca acaaggtatg gttaagggga actttgccaa cgaaggagag
      901 aatcacatgc tttatgtaac gagacacgga gaaacaactt ggaacgcaca aggattagtt
      961 tgcggacggg cagatgtgcc gctgactgaa aaaggacaga tgcaggcaca gaaactagca
     1021 gaaaaggtag tagatttgcc tgttccaatc acgaaaatca tccattcccc tcttcaacga
     1081 gcaagagata cagcacaagc agttgctgat cgtctcagct tgccgctgac cgttgatgaa
     1141 cgattggtag aaatggattt tggggattat gatggactgc caagcaaaga tgagaacttt
     1201 caaaaagcac gtttagcgtt cgctgtacga ttcccaaatg gggagtcagt cttagatgtg
     1261 tatgcacgga tcgttccttt attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg
     1321 ttagtttgcc ataatgcctt gataagagtg atcaatgctt attttcatcc gatgcctaat
     1381 gaggggttct ttacattcat ggtagataat acagaattgg tttcttatga gtaattatgt
     1441 ttttgtgtta tttaaataaa atatttcaag gcactgcttt tgcttagcag tgcctttttt
     1501 aggttatgac aattcttttg ataatataaa ctcaaccttt agaaatgaaa tttaattatt
     1561 ttaataaaag tattgaaatt ttgtttcttt agatatatac tctagtcata gaaatgaaga
     1621 cggatacaga aaaagaggga taatatgttt ggcttttcaa tatttatgaa cgattctttg
     1681 tcagaagaga agaaacagta tatccgccaa atggcatcca atggctttgt cggtattttc
     1741 acttccatgc atataccaga agatgatgtt tcggcatatc aaaagcgatt gatcgaacta
     1801 ggaagcgaag cgaaaaagaa taaactagaa ttgatggtcg atatttcagg agaggcattg
     1861 gaacgagcgg gtttttcatt agaagatcta acgcctttga aagcaattgg tgtaacaggt
     1921 ttaagaatgg attatcatat ttccaatcag cagattgccg actggtcaca tcaattgaaa
     1981 atcagtttga atgctagcac gatcacgcct aaagatatcg atgaattgaa agaagcagaa
     2041 gctgatttct cacagattga agcttggcat aattattatc cacgtcccga aacagggctg
     2101 gacaaagaat ggtatcagaa aaaaaatcaa tggctcaaaa ttcaaggtct ccttgtccaa
     2161 ggttttgtac caggagatac agagttgaga ggcccactat atcaaggatt gccaactttg
     2221 gaagaacatc gagggatcca tcctcttgca ggcgctctag atctattagc aagtaacacg
     2281 gatattgttt acatcggaga tgcaggtctg tcagaaaacg ttcaggagca atttgcttct
     2341 ttccaaaaag aacagacagt cttgctccat acggaacctg tagatgaaga attttacgaa
     2401 tatatattag ggaagcatac gaatcggcaa gacgatgcaa gagatgtcat acgaagcgta
     2461 gatgcacgtt ttcgagagat tccgcctatc ccagcaagaa atacagctac gcgtatgaaa
     2521 gggtctatta ctttagataa cgaaaagtat ttgcgctata tgggtgagat ccaattaacc
     2581 aaatatgatt tgccagcgga tgaaaaagtg aatgtagtag ctaaagttat caaagaagag
     2641 ctaccattga tcaaccaaat caaagctggt atgaactatc aatttataag aaaagaagga
     2701 agataaaaat gagcaaaatc aatttagaga acctaacaac ggaacgcaga aaccaagaaa
     2761 cttttggatt agatgagatg agtatttctc aagccgttca actaatgaac aaagaagatc
     2821 agaaagtggc cgctgcggtt gaaaaagaac tgacaagtat cgaaccagtg attgctaaca
     2881 cgattgagtc ctttaaaaaa ggtggacgtc tgatttatat gggagccgga acaagtggaa
     2941 gactaggtgt gttagatgct gctgaatgcg taccgacatt cggtgtagaa ccggaaatgg
     3001 tcataggtct aattgctggt ggagaagaag caatgactgt agctgtcgaa ggggccgaag
     3061 attcttatga gctagggcaa gaagatctaa tcaagctgaa tttgacaaaa aatgatatag
     3121 ttattggtat cgcagctagc ggtcgtacac catatgtgat cggtggatta gattacgcaa
     3181 aatcgatcgg tgctgtcaca ggaacgattt cttgcaataa acaagccgaa atcagtaaac
     3241 acgcggattt cccaatcgaa gtcgatgcag gtccagaatt tttgacaggc tctactcgat
     3301 tgaagtcagg aacagcacaa aaacttatct tgaatatgat ttctactatc agtatgattg
     3361 gtataggtaa agtattcaat aacttgatgg tcgatgtcaa accaacaaat gaaaaactaa
     3421 tcgaacgctc aaaacgaatc atcatgcaag cgacagaagc tgattacgaa acagctgaaa
     3481 agtactttga agaagcagat caaaatgtta agctggctat cgtcatgatt ttgacaaata
     3541 gtgataaaga ggaagctcaa gcaaaattag ctgaagcaaa cggatttgta aaagggacct
     3601 tatgaggggg aaaagaccat ggcaaaaaac gaactgactt tggaagaact ggcaaaacaa
     3661 atttataaag gggcaggcgg catgggcaat gtagaaagca ttgtccactg catgactcgt
     3721 gtgcgtatga agatcatcaa tgatgatctt gtaaatgtct ctgaattaaa agcgattcca
     3781 ggagtgcttg gtgtagtaga agatgaacag cttcaagtca tcatcggacc tgggaaagtg
     3841 aacaaagtag cacagcaaat ggttgatttg gcaggcgtga aattaggaga aaaactttct
     3901 tctgtaagta cagctacagc aaattcatca gcatctggaa aagaccgtgt aaacgaacgg
     3961 gcacaagaaa tgaaagcggc acaaaaatcg aaacaaaaac catctaaaat caaatcggta
     4021 ttaaaagaca tttctaatat ctttgtgcca atgattcctg catttgtcgg tactgggatc
     4081 gtggcaggga ttgccgcagt tttagcaaac ttagtcacag ccggaacact tgatgcagca
     4141 acatggcaac aatatatcga tgtgatgaac atcttgaaaa acgggatgtt tagctaccta
     4201 gttatctata caggtatcaa tgctgcacaa gtttttggtg caacaccaac tcttggtgga
     4261 gttatcgggg ccgttgtgat gctgacaggg atgaatccgg aagcaccact gaaaaactta
     4321 tttactggtg aagctcttgc agctggacaa ggtgggatcc taggtgtcat cttcgctgtt
     4381 tggctattgt caatcgttga gaaaaaactg cataaagtag tacctgatgc ggtagatatc
     4441 atcgttacac caactctttc gttgattgcc atcggtctga ttgaaatttt cttcatcatg
     4501 cctttagcag gtttcatttc tgatggcatg gtcggtggga tcaactgggt acttggcgtt
     4561 ggtggagcat ttgccggctt cgttctaggc acattattct tgccgatggt tatgttcgga
     4621 ctgcatcaaa ttttgactcc aatccatgtg caaatgattg atgaaactgg tcgtacgcta
     4681 ttgcttccaa ttcttgcgat ggctggtgca ggacaagtgg gtgcagcatt ggcattatgg
     4741 attcgctgca aaaaagataa agaattaaca gaaatgatca aaggagcttt accagtcggg
     4801 atcctaggaa tcggcgaacc attgatttac ggagtaacat tacctctagg acgcccattt
     4861 attactgctt gtatcggtgg aggtatcggc ggggcag
//
LOCUS       DACEOE010000396         4707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.guided.174, whole genome shotgun sequence.
ACCESSION   DACEOE010000396 DACEOE010000000
VERSION     DACEOE010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.guided.174"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1367
                     /locus_tag="IW774_002989"
     CDS             <1..1367
                     /locus_tag="IW774_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1155279.1"
                     /translation="VLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            1379..2326
                     /locus_tag="IW774_002990"
     CDS             1379..2326
                     /locus_tag="IW774_002990"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1155280.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2346..2873
                     /locus_tag="IW774_002991"
     CDS             2346..2873
                     /locus_tag="IW774_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1155281.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(2936..3589)
                     /locus_tag="IW774_002992"
     CDS             complement(2936..3589)
                     /locus_tag="IW774_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1155282.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            3721..4563
                     /locus_tag="IW774_002993"
     CDS             3721..4563
                     /locus_tag="IW774_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1155283.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
BASE COUNT         1680 a          823 c          989 g         1215 t
ORIGIN      
        1 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
       61 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
      121 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
      181 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
      241 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
      301 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
      361 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
      421 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
      481 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
      541 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
      601 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
      661 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
      721 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
      781 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
      841 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
      901 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
      961 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     1021 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     1081 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     1141 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     1201 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     1261 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     1321 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     1381 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     1441 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     1501 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     1561 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     1621 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     1681 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     1741 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     1801 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     1861 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     1921 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     1981 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     2041 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     2101 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     2161 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     2221 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     2281 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     2341 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     2401 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     2461 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     2521 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     2581 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     2641 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     2701 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     2761 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     2821 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     2881 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     2941 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     3001 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     3061 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     3121 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     3181 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     3241 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     3301 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     3361 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     3421 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     3481 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     3541 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     3601 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     3661 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     3721 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     3781 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     3841 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     3901 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     3961 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     4021 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     4081 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     4141 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     4201 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     4261 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     4321 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     4381 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     4441 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     4501 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     4561 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     4621 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     4681 ttgataacat tagaaccaat cgaatac
//
LOCUS       DACEOE010000397         4569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.guided.182, whole genome shotgun sequence.
ACCESSION   DACEOE010000397 DACEOE010000000
VERSION     DACEOE010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.guided.182"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            67..978
                     /locus_tag="IW774_002994"
     CDS             67..978
                     /locus_tag="IW774_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1155284.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1131..1616
                     /gene="vanZ"
                     /locus_tag="IW774_002995"
     CDS             1131..1616
                     /gene="vanZ"
                     /locus_tag="IW774_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1155285.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1903..2232)
                     /locus_tag="IW774_002996"
     CDS             complement(1903..2232)
                     /locus_tag="IW774_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1155286.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2537..2866
                     /locus_tag="IW774_002997"
     CDS             2537..2866
                     /locus_tag="IW774_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1155287.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2856..3143
                     /locus_tag="IW774_002998"
     CDS             2856..3143
                     /locus_tag="IW774_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1155288.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3422..3985)
                     /locus_tag="IW774_002999"
     CDS             complement(3422..3985)
                     /locus_tag="IW774_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1155289.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1611 a          685 c          765 g         1508 t
ORIGIN      
        1 ctaagatttc tctttaccaa catcatttga caaagagcca ctatatagtt aaggaggaga
       61 ctgaaaatga agaagttgtt ttttttattg ttattgttat tcttaatata cttaggttat
      121 gactacgtta atgaagcact gttttctcag gaaaaagtcg aatttcaaaa ttatgatcaa
      181 aatcccaaag aacatttaga aaatagtggg acttctgaaa atacccaaga gaaaacaatt
      241 acagaagaac aggtttatca aggaaatctg ctattaatca atagtaaata tcctgttcgc
      301 caagaaagtg tgaagtcaga tatcgtgaat ttatctaaac atgacgaatt aataaatgga
      361 tacgggttgc ttgatagtaa tatttatatg tcaaaagaaa tagcacaaaa attttcagag
      421 atggtcaatg atgctgtaaa gggtggcgtt agtcatttta ttattaatag tggctatcga
      481 gactttgatg agcaaagtgt gctttaccaa gaaatggggg ctgagtatgc cttaccagca
      541 ggttatagtg agcataattc aggtttatca ctagatgtag gatcaagctt gacgaaaatg
      601 gaacgagccc ctgaaggaaa gtggatagaa gaaaatgctt ggaaatacgg gttcatttta
      661 cgttatccag aggacaaaac agagttaaca ggaattcaat atgaaccatg gcatattcgc
      721 tatgttggtt taccacatag tgcgattatg aaagaaaaga atttcgttct cgaggaatat
      781 atggattacc taaaagaaga aaaaaccatt tctgttagtg taaatgggga aaaatatgag
      841 atcttttatt atcctgttac taaaaatacc accattcatg tgccgactaa tcttcgttat
      901 gagatatcag gaaacaatat agacggtgta attgtgacag tgtttcccgg atcaacacat
      961 actaattcaa ggaggtaagg atggcggaat gaaaccaacg aaattaatga acagcattat
     1021 tgtactagca cttttggggt aacgttagct ttttaattta aaacccacgt taactaggac
     1081 attgctatac taatgataca acttaaacaa aagaattaga ggaaattata ttgggaaaaa
     1141 tattatctag aggattgcta gctttatatt tagtgacact aatctggtta gtgttattca
     1201 aattacaata caatatttta tcagtattta attatcatca aagaagtctt aacttgactc
     1261 catttactgc tactgggaat ttcagagaga tgatagataa tgttataatc tttattccat
     1321 ttggcttgct tttgaatgtc aattttaaag aaatcggatt tttacctaag tttgcttttg
     1381 tactggtttt aagtcttact tttgaaataa ttcaatttat cttcgctatt ggagcgacag
     1441 acataacaga tgtaattaca aatactgttg gaggctttct tggactgaaa ttatatggtt
     1501 taagcaataa gcatatgaat caaaaaaaat tagacagagt tattattttt gtaggtatac
     1561 ttttgctcgt attattgctc gtttaccgta cccatttaag aataaattac gtgtaagatg
     1621 tctaaatcaa gcaatctgat ctttcataca cataaagata ttgaatgaat tggattagat
     1681 ggaaaacggg atgtggggaa actcgcccgt aggtgtgaag tgaggggaaa accggtgata
     1741 aagtaaaaag cttacctaac actatagtaa caaagaaagc ccaattatca attttagtgc
     1801 tgaggaattg gtctctttaa taaatttcct taacgttgta aatccgcatt ttcctgacgg
     1861 tacccccaaa ctaagatttc atagatagat tttctgataa ctttaaaaaa cacgaatagc
     1921 ggaaaggatg tcttcattcg ctaaagagta ataaactact tttccatctt gacgtgattt
     1981 tgccattcca tgttttttta aaaaacgtaa atggtgtgac gttgtagcga cactagcagt
     2041 aagtatttct gctaaatcac agacgcacat ttccttatag gtttctaaag catagaaaat
     2101 tttaattcta gaaaaatcgc tgaaacattt ccctaacaca agtaagtttg aaaaatcttt
     2161 ttgatccaat ttatttttta ttccatttac tttttcttta tgtacgacag tcacttggca
     2221 aacttttttc attcatctca cctcacattc aaacgattat ttgaatgaag tatacctatt
     2281 tgatcactct tttgtcaatc taccttaatt ccaatacact attataagca atttcatgta
     2341 ttgaagtaaa cttacttgca agtgcgtttt tgattccaag ttaaaaacat attattattc
     2401 tacataattt cctaatcaaa taaaactcct ttgaaaattg ttctaaagga gttttattat
     2461 tttgtatttc tactaaaaat agcgtacaat gtaattacta agtaattacg aaatgtaaaa
     2521 taatggagga aataatatga ttacaaaaac aagaaaacaa ggaaattcta ttatgttaac
     2581 ggtccctaaa gaattcgatg tgcccaatgg tgttgaagta gaagctaagc ttgtagagaa
     2641 tggtatcctt tatgagtttg tagagcctaa aaaagaattt tttgatttta gtgaagatat
     2701 tttggctgat attctttcag aaggttatag caaacaagag atcttaaaag aatttagaaa
     2761 tagaaaaaat gcgttaactt ctgcatttaa atctattgct gaagatactg ttgcaaattc
     2821 taaaccgatg acaaaagagg agttggcagc agaaattggc ttatgagatt ctaccttcta
     2881 aacacgtaat aaagtatcta aaaaaattaa aagagaaaac attaaaagaa caatttttaa
     2941 cgataattta tgatgagata gcagttcgtc ctcattcggg agaacaaaaa acgggtgatt
     3001 tatcaggaat ttgggctatt ggattcaagt atgcaggtac gacatatcgc gttgcatatg
     3061 aaattaaaga taatgcagtg atacctatct tactatgtgg aacgcatgaa aatttttatg
     3121 aacaattaaa aaagattagg taagaaccca atgtttcaac ttgagaggat agacgacact
     3181 ttaaaagata atttttaaca gctgagagtt gtaataccct ataattttaa accactaacc
     3241 tatatattta tgaaaaatag aaaaaattaa aacgcatttt aagcctcttt caaattaaaa
     3301 gcttcatcct tatgcacaaa gagggcttaa aatgcgttat tttatattgt tttttagtaa
     3361 taataaaaaa atcactctaa ttctcccata aatattatag attcctaaag attaaaatag
     3421 attattccct attgtctttt aagtagcggt agagtgttgc cttgctaata ccagtcattt
     3481 ccgtaatttc ttttacgcta taaacctgac ttttatacaa ttttactgct cgatcaacat
     3541 ctttctggtt cactcgtgga cggccacctt tacgacctct cgcacgtgca cttttcaaac
     3601 cttctttggt acgttcaaca attaaatcac gttcgaactg actaaatgct tggaagacag
     3661 tcatcattaa gcgtccgtga ggcgtgctgg tatcgaagtt ttctttcaag ctcaccagct
     3721 taacatggtg ttcttcaaaa taggtaacaa ggtcaatcaa atcctttgta ctacgaccca
     3781 atcgagaaaa actttctaca attaccgtat ctcccggacg aattttatct tttaaacgat
     3841 ttagttgggg acgaaccgct ttggttcccg tcattttctc agttaggatt tcattacaat
     3901 tttgttcatt gagtaagtct aattgacgat ttagttcttg tgaacgtgta ctcactcggg
     3961 cataaccata aatgtatcca ctcatgcttt tcttcctttc gtactttaat tctggcttga
     4021 ttgtatcata aaagggagtt atagatacat agttaatgca acaagttatg atacaaaata
     4081 tgacctcttt tatttaataa aagaggtcat ttcattttgt ctcataatcg attgtttttg
     4141 atacgagtgt gtatacgaaa taaacaaaaa aatcttaagt aaatttctag atacctaatt
     4201 taatcctaac tagtgtatcc taaagttaat aacgagttta tcacaatgaa aattataaaa
     4261 tgctcccata ttagatataa catgcaatat ttgaattaga actacaaaat ttattcgttt
     4321 tataaagtaa ataatgttga gatactaagg aatagaatat tataaggaat tgagctgaat
     4381 gtattggata aaaaaagaat aactttatta gagatggact ataaaaatgc tcgtaatttt
     4441 cttctgaagt ctgaaagcta tttgaatgct cagctaccag aatatattag ttttgacgaa
     4501 actttaaaat ctgctaaaaa agttttaact tcgccgtttg tataattaag cgagacaaat
     4561 aaaaaagcc
//
LOCUS       DACEOE010000398         3297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.guided.220, whole genome shotgun sequence.
ACCESSION   DACEOE010000398 DACEOE010000000
VERSION     DACEOE010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.guided.220"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1213
                     /locus_tag="IW774_003000"
     CDS             245..1213
                     /locus_tag="IW774_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1155290.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IW774_003001"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IW774_003001"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1155291.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IW774_003002"
     CDS             2243..2851
                     /locus_tag="IW774_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1155292.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          980 a          632 c          795 g          890 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag gctctatact ttttgatgtt ggtggatgac accagca
//
LOCUS       DACEOE010000399         3253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.guided.223, whole genome shotgun sequence.
ACCESSION   DACEOE010000399 DACEOE010000000
VERSION     DACEOE010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.guided.223"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            157..1371
                     /locus_tag="IW774_003003"
     CDS             157..1371
                     /locus_tag="IW774_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1155293.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1402..1620
                     /locus_tag="IW774_003004"
                     /pseudo
     CDS             1402..1620
                     /locus_tag="IW774_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2039..2512
                     /gene="dfrF"
                     /locus_tag="IW774_003005"
     CDS             2039..2512
                     /gene="dfrF"
                     /locus_tag="IW774_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1155294.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2639..>3253
                     /locus_tag="IW774_003006"
     CDS             2639..>3253
                     /locus_tag="IW774_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1155295.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMH"
BASE COUNT         1058 a          482 c          700 g         1013 t
ORIGIN      
        1 gtctttattt gtgtgaaaaa cgaaaaagag cacaacacat caagctttta ttaattgtct
       61 gtcttttata aatgcaagta ttaattcaaa aaaacagaag aaaaaaacaa ctacaccttc
      121 ttttatactc gtaatcgtag aaaggaggcg aaaaaaatga atacattacc tatcaataca
      181 ggagatacag cattcatgat tttatgtaca gcaatggttt gcctgatgac tcctggtcta
      241 gctttttttt acggcggatt agcacggaag aataatatac taacgatcat gtctcaatct
      301 tttctctccg ttgggataac aacgattatg tgggtatttg gtggattcgg attggccttt
      361 ggacgggata tcggcggtgt tattggaaac ccggcagatt tctttttgat gagacatgtt
      421 acaagcttcc ccaacatgtt tcatggtgca acgattccat ttttaatgtt ctttctttac
      481 caactgatgt ttatcgtgat taccgttcct ttgatgacag gagcttttgc agggagattg
      541 aatttaaaag gctatattat acttgttatt ttttggaatc tgctgattta tttccctgtt
      601 tgccactgga tatggggtgg cggatttttg gatcagatgg gatttagaga tttcgctggt
      661 ggagcagtca tccacacgac agctggattc ggctctttag catgtatcct cacacttggt
      721 caaagaaaga ttcctttagg agaaagaagt cagcatagca gtcttatggc agctgctatc
      781 ggtacaggac tgctttggtt tggctggttc ggttttaatt ccggtggtgc acttcgagca
      841 gatgaacaag cagtcaatgc atttggttca acattcatcg ctcttgcatt tgcgatgatt
      901 acttggctga tcattgcaaa agtaaaagga aatggctttg attttgtcga cgtactaaca
      961 ggatccgttg ctggattggc agccattacg ccttgtgccg gttatgtaga agcaaaaagt
     1021 gccatgctga ttgggatcat tgcgggtatc gtctgtcatg ctgctgttga ttttcgtaaa
     1081 aagaaacaat gggatgatgc acttgatgtg tggggtgttc atggcatggg cggattcaca
     1141 ggtacgattc tgatagggat ctttgcatct aacagccatt tactaacgaa tccaagcagc
     1201 tggtacttct tagggattca agtgattggt gtcatcatta ctgccgtgta tgcctatgtg
     1261 ctgacaacag tgattttaaa atcggcgatg cactttacaa cgatcactac tacaaaagaa
     1321 gaacaagaag aaggattgga ctttttcttc tttggcaaca aactcaattg aaacaaattc
     1381 aaatagaaaa gaggaagatt catgcaacaa agcaaaacga aaattcctgc aaaaaaatta
     1441 acgttctttg gctttttggc tatgaccatc tcaatggtcg tttcattata tgaatatccc
     1501 acattcgcga cctcaggctt cagcttagta tttttcttgt tattaggcgg tttgctatgg
     1561 tttatccctg tggcattgtg cgcagcagag atggcaacgg tgaaaggatg ggaaaaaggt
     1621 gtttcccaaa caaaaagata ttaagaaaca gcccatgttt acctgcttgc aaacaaaaga
     1681 atattgctac tagactaaga gatactacat aagtgtgttg gtatctcttt tttgttgccg
     1741 tgaaacagca gactatttca tttctgcttg attaccttaa aacttattag tctatggttt
     1801 gtcaagggct tgttgcgtga gaaatgctga atatcaccct ttacaaaaca taggctgata
     1861 agtaactttt caaaagcaga aaagaaatgg caatgcttat aacaatatga tataatgcag
     1921 atgaagacaa aaaaggaatt tgtcgagctg gttagttcga gataattttg agtttgttaa
     1981 gatgtggtgc acttgatgga atcacaggta cgacatcata ttatggagga tgaaatagat
     2041 gataggtttg attgttgcga ggtcaaagaa taatgttata ggcaagaatg gtaatatacc
     2101 atggaaaata aagggagaac aaaagcaatt tagagagtta acaacgggta atgtggttat
     2161 tatggggcga aagtcttatg aagaaatcgg tcatccgttg cctaatagaa tgaatattgt
     2221 tgtttccacc acaacagagt atcaaggaga taatttagtt tcagttaaat cattagaaga
     2281 tgcattatta ttggctaaag gacgagatgt atacatatct ggtggatatg gactatttaa
     2341 ggaagctttg caaatagtag ataaaatgta tatcacagaa gtagatttaa atattgaaga
     2401 tggagataca ttctttccag aatttgatat caatgatttt gaagttttga taggggaaac
     2461 acttggtgag gaagtgaaat atacgagaac attttatgta aggaaaaaat gaattgagta
     2521 aattttggat ttagggtgtt ttcataaata tattgctttt tatgcatata taaattgttg
     2581 taagacaaat tccagtttgt atggggcgat aaaatcagaa tttgagagag gattgaatat
     2641 gaataaggaa tggtctgaac ttaataaaac aatgcaggca caaataaaaa agaaagatac
     2701 ttataagagg ggtattgata ctttacttac tttacgaagt cagttaatac aaaccttagt
     2761 atctttcaaa gaagaattat gcagagaaga ttttaactca atccccttca taaacgctga
     2821 tggttatcat agtaagacga ttgcttattc tatttggcat attttccgca ttgaagatat
     2881 agttgtgcat acagtgataa atgaagatga acaagtattt tttgcaggca attatcaaga
     2941 gcgtatcaat tcacctatca ttacaacagg aaatgaactc atgaaacagc agattgcaga
     3001 cttttcaaaa cagcttaatc tggaagaatt atatttatat attttcgaag tatgggaaag
     3061 cactgagaaa atgcttgaac ggttatctta tgatgaattg aaaagaaaaa taccgaaaga
     3121 aagaaaagga tacttagaat cgttgaatgt agtaaacgac aatgaaaaag caatttggct
     3181 gattgattat tggtgtaata aagatatttg tggactaatt caaatgccgt tttcaagaca
     3241 ttggattatg cac
//
LOCUS       DACEOE010000400         2915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.guided.236, whole genome shotgun sequence.
ACCESSION   DACEOE010000400 DACEOE010000000
VERSION     DACEOE010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.guided.236"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            75..788
                     /locus_tag="IW774_003007"
     CDS             75..788
                     /locus_tag="IW774_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014124381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1155296.1"
                     /translation="MQKNAKTLERRLQKIGETTKPQQMKQIRFPVPKSLELHSRYPIM
                     GQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGSGKTTLLEHIRKQGEGILLSPKVS
                     FQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSLLNHLGFAQETLTKPLCTLSGGEA
                     TRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEKLMQIYPGTILFTSHDSYFVERTA
                     DEVYEIKGQKIKKVLTRNF"
     gene            835..1281
                     /locus_tag="IW774_003008"
     CDS             835..1281
                     /locus_tag="IW774_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1155297.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(1480..1995)
                     /locus_tag="IW774_003009"
     CDS             complement(1480..1995)
                     /locus_tag="IW774_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1155298.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2112..>2915
                     /locus_tag="IW774_003010"
     CDS             2112..>2915
                     /locus_tag="IW774_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1155299.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASI"
BASE COUNT          845 a          498 c          631 g          941 t
ORIGIN      
        1 gtaaaaagaa aaagcaacaa atcagaccgg atcggttgtc ttcctctaaa caaaaagaca
       61 gtgtacaaaa agccatccaa aagaatgcga aaacattaga gagaagactc caaaaaatag
      121 gagaaacaac caagccgcag cagatgaaac aaatccgttt tccagtacca aaatctcttg
      181 aactccacag ccgctatccc atcatgggac aaaatgtcca attggaaaga agtgggagaa
      241 cattactggt aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg
      301 aaaatggttc aggtaagaca accttattag aacatatccg caaacaagga gaaggaatcc
      361 ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa atgacatctg
      421 aagaatccat cattcgtttt gtcatgagac aaacagagtt ttcagaatcg cttgtccgta
      481 gtttgctgaa tcacttaggg tttgctcagg aaactttgac gaaaccgtta tgtacattaa
      541 gtgggggaga agcgacccgt ctgacgattg ctttgctttt tactaagcca agtaatgtgt
      601 tgctgttaga tgaaccgact aattttattg atatggcaac gatcgaagct ttagagaagc
      661 taatgcaaat atatccggga acgattttgt ttacttctca tgattcttac tttgtcgagc
      721 gtacggctga tgaagtttat gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa
      781 atttttaaat gtgctacgat gattttcgtg acaaatttaa aggagtggta cagattgcct
      841 acaaataaaa aagaaggaat aatttttaca acaatgatgt gttttttgat ggtcttgggg
      901 atgagcgcgt ataatttatg gctgcatcaa gatcttcatt tatcaagttt gttgattggt
      961 ctagtgcctg gattcgtggt ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg
     1021 aaaaaaattg cctttaaact accagtaaat ccagaaagca aggtccaaat gatcttaact
     1081 atttcatgct tgatggtttt aggaatggtg acatgtatgt ctttattcgg tgtgatcatg
     1141 gaacaaggaa tacctgatca attattctct gcttatatga atgcttggaa aatgaatgtg
     1201 attgccgctt taccgttgca gttgatcatc gttggcccgc tttcaagaag tgtgctaaaa
     1261 gcaattcaaa gtaatgaatg atggaacact gttatttgaa aaaaggcctt ggcatgcttt
     1321 ttgtcatagt ctcttgaatg cagttctaga aaaatagtga tccgaacgat aggttttggc
     1381 ctgtcgttcg gatcgctttt tcttttccaa ctgtattgct cttttattga agataatttg
     1441 ctcttcaata gatatttcta gaaaggttct gtttatccct tactcaaaaa actgctgcat
     1501 ttgttctctt acttgctgac accattggag ttcctgctga caaagcataa ttctgcgctg
     1561 gatcacaaca taacgtccaa attcttgttt cttgtctagc gagaggtcat tcaatttttt
     1621 ttgatttttt tgcatgattt tgttcagctg ttgttctcta gtgaatagca gtccagtagc
     1681 tgttgtttta tcgagcgtcg aaaaagcata gatttttgct agaaattcat ctttttgcgt
     1741 aggagcggga gtttcctctt tgatccattc ctctacaaga gcacgtcctt catcagtcaa
     1801 gtggtagatc tttttttgag agtgctcgtc tttgtccaaa acggtcacat aaccctgctg
     1861 gtctaacttg gcaagcgttg tatagatctg actgtgatgg gcttcccaaa aaattgtgat
     1921 cagctgcttc aattcatacc cagaacgtgg tttggataaa agcacgcata ataatatata
     1981 ggctaaagta ttcataaaaa tcctacctct cgtcttattt tctttattat atcacagtaa
     2041 agtttccacg ttttacatgt gcatatttac atgtaaaata aatcatgtat gatgctaata
     2101 gagagaaagg gatggaaata ttggagaatc aaatagatat acaagaacaa acaaatgaag
     2161 aaacaaaggg gaaaagttca gcgctagttg tagcagctac ctgtatgatc gcttttatgg
     2221 gagtgggtct agtagaccca atcttaaaaa cgattgctgc acagttaaac gctacgccag
     2281 ctgaaacgac cctgttattt actagttaca tgttagtcac tggtgtggtg atgctattta
     2341 caggtttctt atctagtcgg atcggtttga aaaaaacatt gatgacagga ttgtttatca
     2401 tcgttgtatt cgctggtctt ggcggattat ctgggaatat cggaatgctg attgggttac
     2461 gtgccggctg gggtgtcggg aatgctttat ttgtctctac tgctttagca acgatcgtca
     2521 gtattttagt tggtaacaca gaaaaagcaa tcatgatgta tgaagcagca cttggctgcg
     2581 gaatggctgt tggccctcta gtcggtgggg tcttgggcag tggttcatgg cgttatccat
     2641 tttttggggt agcatttttg atgttttgcg gttttatagc cctagcggta cttttacctg
     2701 aaactgagaa accaaagaga aaagtaggcc tttgggaagg tgtcaaagcg ttaagcaatc
     2761 ataaacttcg ttcagttgga ctaatcgcat tgttatataa tttcggattc tttactttac
     2821 ttgcgtatgc gccattttta ttgattggct attctgaatt agaagtcggt tttgtatttt
     2881 ttggctgggg tgtgcttctg gctattgctt cgatt
//
LOCUS       DACEOE010000401         2242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E327
            SAMN07141420-rid9868513.guided.268, whole genome shotgun sequence.
ACCESSION   DACEOE010000401 DACEOE010000000
VERSION     DACEOE010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141420
            Sequence Read Archive: SRR5811401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740731.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,013
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 31 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 9 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141420-rid9868513.guided.268"
                     /isolate="E327"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..148
                     /locus_tag="IW774_003011"
     CDS             <1..148
                     /locus_tag="IW774_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1155300.1"
                     /translation="LIVDLKLCNRTNYAVKLYKEGNMTVNQICEITNVSRASLYRKLS
                     EVNN"
     gene            362..1057
                     /gene="vanR"
                     /locus_tag="IW774_003012"
     CDS             362..1057
                     /gene="vanR"
                     /locus_tag="IW774_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1155301.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1035..2189
                     /locus_tag="IW774_003013"
     CDS             1035..2189
                     /locus_tag="IW774_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1155302.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          757 a          409 c          475 g          601 t
ORIGIN      
        1 tttgatagta gatttaaaac tttgcaacag aaccaattat gcggtaaagc tatataaaga
       61 aggaaatatg actgtaaatc aaatttgtga aattactaat gtatctaggg cttcattata
      121 caggaaatta tcagaagtga ataattagcc attctgtatt ccgctaatgg gcaatatttt
      181 taaagaagaa aaggaaacta taaaatatta acagcctcct agcgatgccg aaaagccctt
      241 tgataaaaaa agaatcatca tcttaagaaa ttcttagtca tttattatgt aaatgcttat
      301 aaattcggcc ctataatctg ataaattatt aagggcaaac ttatgtgaaa gggtgataac
      361 tatgagcgat aaaatactta ttgtggatga tgaacatgaa attgccgatt tggttgaatt
      421 atacttaaaa aacgagaatt atacggtttt caaatactat accgccaaag aagcattgga
      481 atgtatagac aagtctgaga ttgaccttgc catattggac atcatgcttc ccggcacaag
      541 cggccttact atctgtcaaa aaataaggga caagcacacc tatccgatta tcatgctgac
      601 cgggaaagat acagaggtag ataaaattac agggttaaca atcggcgcgg atgattatat
      661 aacgaagccc tttcgcccac tggagttaat tgctcgggta aaggcccagt tgcgccgata
      721 caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt atcgtccact ccggccttgt
      781 cattaatgtt aacacccatg agtgttatct gaacgagaag cagttatccc ttactcccac
      841 cgagttttca atactgcgaa tcctctgtga aaacaagggg aatgtggtta gctccgagct
      901 gctatttcat gagatatggg gcgacgaata tttcagcaag agcaacaaca ccatcaccgt
      961 gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt gataatccga aatatataaa
     1021 aacggtatgg ggggttggtt ataaaattga aaaataaaaa aaacgactat tccaaactag
     1081 aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt
     1141 atattcgttc aatgatccga gggaaacttg gggattggat cttaagtatt ttggaaaaca
     1201 aatatgactt aaatcacctg gacgcgatga aattatatca atattccata cggaacaata
     1261 tagatatctt tatttatgtg gcgattgtca ttagtattct tattctatgt cgcgtcatgc
     1321 tttcaaaatt cgcaaaatac tttgacgaga taaataccgg cattgatgta cttattcaga
     1381 acgaagataa acaaattgag ctttctgcgg aaatggatgt tatggaacaa aagctcaaca
     1441 cattaaaacg gactctggaa aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa
     1501 atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc ccttacatcc attatcggtt
     1561 atttgagcct gcttgacgag gctccagaca tgccggtaga tcaaaaggca aagtatgtgc
     1621 atatcacgtt ggacaaagcg tatcgactcg aacagctaat cgacgagttt tttgagatta
     1681 cacggtataa cctacaaacg ataacgctaa caaaaacgca catagaccta tactatatgc
     1741 tggtgcagat gaccgatgaa ttttatcctc agctttccgc acatggaaaa caggcggtta
     1801 ttcacgcccc cgaggatctg accgtgtccg gcgaccctga taaactcgcg agagtcttta
     1861 acaacatttt gaaaaacgcc gctgcataca gtgaggataa cagcatcatt gacattaccg
     1921 cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa cactggaagc atcccaaaag
     1981 ataagctagc tgccatattt gaaaagttct ataggctgga caatgctcgt tcttccgata
     2041 cgggtggcgc gggacttgga ttggcgattg caaaagaaat tattgttcag catggagggc
     2101 agatttacgc ggaaagcaat gataactata cgacgtttag ggtagagctt ccagcgatgc
     2161 cagacttggt tgataaaagg aggtcctaag agatgtatat aattttggct ctttgtcaaa
     2221 tgatgttggt aaagagaaat ct
//
